LOCUS       DAZXUL010000001        74777 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZXUL010000001 DAZXUL010000000
VERSION     DAZXUL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74777
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.001"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..134)
                     /locus_tag="ACH3L1_000001"
     CDS             complement(<1..134)
                     /locus_tag="ACH3L1_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4074939.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSAS
                     G"
     gene            complement(131..1033)
                     /locus_tag="ACH3L1_000002"
     CDS             complement(131..1033)
                     /locus_tag="ACH3L1_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFT4074940.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEELPDDVRETITVHFTAKRGDRCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIIDNVAHYGG"
     gene            1452..1700
                     /locus_tag="ACH3L1_000003"
     CDS             1452..1700
                     /locus_tag="ACH3L1_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFT4074941.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            1697..2134
                     /gene="umuD"
                     /locus_tag="ACH3L1_000004"
     CDS             1697..2134
                     /gene="umuD"
                     /locus_tag="ACH3L1_000004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFT4074942.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            2134..3405
                     /locus_tag="ACH3L1_000005"
     CDS             2134..3405
                     /locus_tag="ACH3L1_000005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFT4074943.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEIFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSPAYTTRWTDLPVAQL"
     gene            3617..4243
                     /locus_tag="ACH3L1_000006"
     CDS             3617..4243
                     /locus_tag="ACH3L1_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HFT4074944.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
     gene            4240..4542
                     /locus_tag="ACH3L1_000007"
     CDS             4240..4542
                     /locus_tag="ACH3L1_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4074945.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            4933..5118
                     /locus_tag="ACH3L1_000008"
                     /pseudo
     CDS             4933..5118
                     /locus_tag="ACH3L1_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5138..5485)
                     /locus_tag="ACH3L1_000009"
     CDS             complement(5138..5485)
                     /locus_tag="ACH3L1_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFT4074946.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLCMASFHWW
                     RRKVNRLPPWDDV"
     gene            5614..5949
                     /locus_tag="ACH3L1_000010"
     CDS             5614..5949
                     /locus_tag="ACH3L1_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFT4074947.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSGFFADTPGKMGLLAVYTGTVFILSLPLSMIYTLS
                     VIIKRLSVR"
     gene            complement(5971..7851)
                     /locus_tag="ACH3L1_000011"
     CDS             complement(5971..7851)
                     /locus_tag="ACH3L1_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFT4074948.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAVREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNLSARTNEQKHANDALNALLKEKENIRNQLAGINQKIAEEKRKQDELKETK
                     DAINFTTEFLKSVSEKYGAKAEQLAREMAGQAKGKKIRNVEEALKTYEKYRADINKKI
                     NAKDRAAIAAALESVKLSDISSNLNRFSRGLGYAGKFTSLADWITEFGKAVRTENWRP
                     LFVKTEAIIAGNAATALVALVFSILTGSALGIIGYGLLMAVTGALIDESLVEKANKFW
                     GI"
     gene            complement(8130..9476)
                     /gene="yagA"
                     /locus_tag="ACH3L1_000012"
     CDS             complement(8130..9476)
                     /gene="yagA"
                     /locus_tag="ACH3L1_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFT4074949.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFKKHMYGVFMHLRY"
     gene            9830..10432
                     /locus_tag="ACH3L1_000013"
     CDS             9830..10432
                     /locus_tag="ACH3L1_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFT4074950.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSLPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAAARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            10488..10963
                     /locus_tag="ACH3L1_000014"
                     /pseudo
     CDS             10488..10963
                     /locus_tag="ACH3L1_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11123..12661)
                     /locus_tag="ACH3L1_000015"
     CDS             complement(11123..12661)
                     /locus_tag="ACH3L1_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HFT4074951.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPTVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQSEMYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQPHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
     gene            complement(12710..13057)
                     /gene="tnpB"
                     /locus_tag="ACH3L1_000016"
     CDS             complement(12710..13057)
                     /gene="tnpB"
                     /locus_tag="ACH3L1_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFT4074952.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEDIDW
                     RQPKRLLTSLTML"
     gene            complement(13054..13458)
                     /locus_tag="ACH3L1_000017"
     CDS             complement(13054..13458)
                     /locus_tag="ACH3L1_000017"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4074953.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLN
                     ISCEVTFRHGSLRLNGAISENILNLLIRELKR"
     gene            complement(13536..16514)
                     /locus_tag="ACH3L1_000018"
     CDS             complement(13536..16514)
                     /locus_tag="ACH3L1_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnEc1 family transposase"
                     /protein_id="HFT4074954.1"
                     /translation="MARRQILSQSERESLLALPDDELTLTRMAYFSEHDLALINAHRK
                     PASRFGFAVLLCYLKNVGFAPDKKTPPSDALLKPIANRLKLADGLWSSYINGRETTRR
                     EHLTELYRYLGVKAFTNKIQQSCITHLLPMATRTDKGILLAEEMLAYLRQNKVMFPAI
                     DVVERTCAEAMAGGDKIVFQTLNAPLTSAHREALDRLLESSDNQPSRLTWLLQPPGKI
                     NGKNVLQHIDRLNSIESLALPEGIDRTIHQNRLLKLAREGRKMSSRDLTRFSAARRHA
                     ILVCVLEDARATLTDEIIELHERILNSLFNKAKKTQAERLQQTGRLIQLKLKQYIDIG
                     QALSEAHDSGEDPWLAIEKILPWPEFIASLEETRHLARKNNFDPLHIITEKYSTLRKY
                     APRMLSTLQLVATPAAQALADALLVIKDMYKKQLRKVPATAPLEFVPESWRKVVITPT
                     GIDRQYYEFCALSELKGALRSGDIWVKGSRRYKNFDDYLIPEKDFDKLSPALPLPVSA
                     DYHEYITNRMILLQSKLEEVNKMAAHGELPDVEISDKGVKISPLDNCVPLQVSPLSEL
                     IYSMLPRPKITEILDEVNSWTAFTRHFSHIKNDMTRPDTRLLLTTILADGINLGLTKM
                     AEACPGCTKSSLEDIQAWYIRDETYSAALAELVNAQGKRPLAAFWGDGTTSSSDGQNF
                     RTGNSGRYAGQINPKYGQEPGCQFYTHISDQYSPFYTCIISRVRDSTHVLDGLLYHES
                     DLDIREHYTDTAGFTDHVFALMHLLGFAFCPRIRDLHDKKLFIKGKADQYPALHSLIS
                     PTRINLKEIEIHWREVLRLATSIKQGTVTASLMLRKLASYPKQNGLAKALREIGRIER
                     TLFMLDWFRDPALRRRVQAGLNKGEARNALARAVFMHRLGEIRDRKPENQSYRASGLT
                     LLTAAISLWNTVYMERAVDALKRKGMKINAQLLSHLSPLGWEHINLTGDYIWKNNRIP
                     TSGKFRRLRSVKIDKVKKQP"
     gene            16669..17310
                     /locus_tag="ACH3L1_000019"
     CDS             16669..17310
                     /locus_tag="ACH3L1_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFT4074955.1"
                     /translation="MFIRAYLRASTEDQFADRAKEMLEQFVQGRGHKIASYYRENISG
                     TKLDRPELGRLLMDSHRNDILLVEQIDRLTRLSNSDWITLKKQIEQHELRIVSLDVPT
                     SWQALSDKAPSQSDPITRAVITAINNMLIDLMAAMSHKDWLSRRQRQKQGIERAHTLG
                     KYRGKQADQERHQKVLYYRQVKKLSIRETADATGYSTSQVCRIQALYREPTTD"
     gene            17356..17550
                     /locus_tag="ACH3L1_000020"
                     /pseudo
     CDS             17356..17550
                     /locus_tag="ACH3L1_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17742..18023)
                     /locus_tag="ACH3L1_000021"
     CDS             complement(17742..18023)
                     /locus_tag="ACH3L1_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4074956.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKAVAEMFHNAAWGVQTLW
                     FELVTKIDMDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKSPE"
     gene            complement(18129..18404)
                     /locus_tag="ACH3L1_000022"
     CDS             complement(18129..18404)
                     /locus_tag="ACH3L1_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFT4074957.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(18404..18688)
                     /locus_tag="ACH3L1_000023"
     CDS             complement(18404..18688)
                     /locus_tag="ACH3L1_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFT4074958.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(19587..20465)
                     /gene="repA"
                     /locus_tag="ACH3L1_000024"
     CDS             complement(19587..20465)
                     /gene="repA"
                     /locus_tag="ACH3L1_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFT4074959.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENRLRKKQGMDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASPLV"
     gene            21168..21809
                     /locus_tag="ACH3L1_000025"
     CDS             21168..21809
                     /locus_tag="ACH3L1_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFT4074960.1"
                     /translation="MCLSELQARLNVGQPLFPTHALCIRRSQQHRSPPAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            21950..22612
                     /locus_tag="ACH3L1_000026"
     CDS             21950..22612
                     /locus_tag="ACH3L1_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFT4074961.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALATC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTGDTFSTTGRMIRPVAFSLIHP
                     ATINTSEVEEIFRDEGFMLAHGRECSLNGSGRNMLSRILHVGHSGLAEEEWGMDNSLL
                     LH"
     gene            22879..23499
                     /locus_tag="ACH3L1_000027"
     CDS             22879..23499
                     /locus_tag="ACH3L1_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFT4074962.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            23526..25220
                     /locus_tag="ACH3L1_000028"
     CDS             23526..25220
                     /locus_tag="ACH3L1_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFT4074963.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTARVHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            25301..25543
                     /locus_tag="ACH3L1_000029"
     CDS             25301..25543
                     /locus_tag="ACH3L1_000029"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFT4074964.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            25659..25994
                     /locus_tag="ACH3L1_000030"
     CDS             25659..25994
                     /locus_tag="ACH3L1_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4074965.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFTIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GHVSITGKK"
     gene            26221..27291
                     /locus_tag="ACH3L1_000031"
     CDS             26221..27291
                     /locus_tag="ACH3L1_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFT4074966.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREAHQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSAATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVPPQQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPAE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            27295..27732
                     /gene="pilM"
                     /locus_tag="ACH3L1_000032"
     CDS             27295..27732
                     /gene="pilM"
                     /locus_tag="ACH3L1_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFT4074967.1"
                     /translation="MGWVTGFIMVIMAIGNFWFHSHLSRTVHHQQTAEITQQAADFIR
                     YMNAINDYLYQHPERRAAGGQLTSAQLGLPATKNVSHLISQQRVFVWAKEKPGLMGAL
                     LEQSGDSALLARVENGRLLDTRGRRISITLPAVIPDQVIIWMN"
     gene            27764..29383
                     /locus_tag="ACH3L1_000033"
     CDS             27764..29383
                     /locus_tag="ACH3L1_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFT4074968.1"
                     /translation="MNKKLLAIMIAATLLQGCTSFRQAENTRKDAVAEQQRAREMIAE
                     LRNRTPVVKESPRQWINPRPVTDKNPGNAVPGCPIIINTRQSITLQQVAQRISQNCHI
                     PVRITPDVQAYLSVTTSGSTQQIQGTVPPPDTGGMVPLAAIGSSDTTLPRLQGNTASL
                     PELKASGLTSLLNAVASRLGISWRYDGSHITFYYLDTRTFPVTYMDSQVAYNATVVSG
                     TMSSNGSSGGTASDAISGDASNTQTTTVDMKSALYQDVKNAVSSMLTPGIGRMFMSTG
                     FITVTDTPQVLETVRTFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDTVTGGVSIVNGKLTGSKAFLKALSSQGDVSVVTRNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            29404..30699
                     /gene="pilO2"
                     /locus_tag="ACH3L1_000034"
     CDS             29404..30699
                     /gene="pilO2"
                     /locus_tag="ACH3L1_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFT4074969.1"
                     /translation="MTDISPTERQVFPLTAGRNVFLAGMDWKTLPASHKNPRTFARSL
                     GAVRYISCEYLSTEDTDRHIMVAAVSQNTLPKGSRRYFSLAMLILPLLEPGGYAIVEL
                     SQANDTELYGFVSAVDGILVSDLVGTREEIREAREIFLTINSAPEHGWTCYEPPSFNG
                     PDGRGPLPLETLTGAGKYPAEARLHPVSRGPQLIPVLLILTLLGTAWYGWQYYERLKA
                     EKAALAEAAQKAAEERITPPWLSMPETGDFIRGCSDVWSRLPVSVAGWRYSLAECAAT
                     GNSGNLRASYTNIAGTTVTAFSQRVREIFSIRPFMVMPDGNSGGFALPVTFSMPETPV
                     AVEALPENTLLQERLTTLARSMQLKMDWQEVSNSFTDEDGNTIQPPWKEYDLQILTTL
                     PAHQVAEHFSEPSVRFISVTRQLEEGRFRYQFTGKYYVQ"
     gene            30689..31147
                     /gene="pilP"
                     /locus_tag="ACH3L1_000035"
     CDS             30689..31147
                     /gene="pilP"
                     /locus_tag="ACH3L1_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFT4074970.1"
                     /translation="MSSKTKALLLSVLILPTTVAASTISNMQQNNRQLQVEQIQEETA
                     LYEAMLAREKVRHELRTLEQGEPAEPVHSQESSSAVTTDTPAPITGIPRLTEISGVHP
                     QITARIQLPGGQTADVKEGQHIPGTTFRVEKITTDSVVLEQNGIRTTLQP"
     gene            31251..32759
                     /locus_tag="ACH3L1_000036"
     CDS             31251..32759
                     /locus_tag="ACH3L1_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFT4074971.1"
                     /translation="MLAPADQPPDDILDYVVVNTDKTGQIHVRVVEGKKHLRAVQDYL
                     TRLCSRYPGKVSPFEFTTLDVIARLRQETVISGVENGINPVQQKMLGYISHAATLSAS
                     DLHITPGRDSTDFTYLEARVHGELELLDIVRKDEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAERFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQHIPTFSMLGYHPEQE
                     RAVRRMLQRPEGIVILSGPTGSGKSTTLRTASAAYLEQYGFNDTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSVKSALRLDPDAILNGEIRDHASAITAIKAAMTG
                     HLMLTTLHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQVICPHCRLPWHEV
                     ESSRTDEERRLVENFCQPDAVYLRNHNGCPHCWRGVNGRTVIAEVISPDAKFFQIYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            32761..33855
                     /locus_tag="ACH3L1_000037"
     CDS             32761..33855
                     /locus_tag="ACH3L1_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFT4074972.1"
                     /translation="MKILERLNNTELELSGLNRWLGKKTFRRTTFYESLAGMIRDGTP
                     VMRALEFICDVETDFGKKKGQSGLYFLATDCIASIRSSGQLSPALKDWVPKDEIALIR
                     NGEERGDIAEAMFQVVKTAKGRQEMISSLVSVCLYPLILLTLCVVNMYNVHTRVIPMI
                     SAFAPEERWSVQMVILAKSSGLVIGYGLWLLAGLLLLAILIRMSFSLYTGPRRRYLDR
                     IPPWSLYQTFHGVNYLFNISSMLQINIPLSHALTRIEKHAEHNRWLKSRISAIRKNVL
                     SGQSLALAMKNCGYDFPSKSCVNNLLLNSEREDSVASMALYADRWLEEAKKNVRKSGI
                     IITAISGLLVFLFIINMVSAIYGISDMLKQ"
     gene            33917..34453
                     /locus_tag="ACH3L1_000038"
     CDS             33917..34453
                     /locus_tag="ACH3L1_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFT4074973.1"
                     /translation="MSVITSSYSEHNIRHLQNDDKGMTLLEVLGVLVVAAIVIGAVMG
                     LMSDTLSSSDNQKELKNLQTIATKMKAQKFQGQYTGTDYVKILTESGGLPADMIAGGN
                     KAKNAWGGAVTIKVSSDKYSYVIESSNVPKKNCIDLVTSLRSSSMFTKINGNVTNKVD
                     PSTVCNADKTTIKLETNS"
     gene            34498..34983
                     /locus_tag="ACH3L1_000039"
     CDS             34498..34983
                     /locus_tag="ACH3L1_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFT4074974.1"
                     /translation="MNRLFILTVIFSLLCSLPSAQASPQDKQYDRCFITAGKRYSIDP
                     GLLKIIARRESSLNPRAINHNRNDRGKIISTDYGIMQINSTHIPGLKKMGIIRSEKDL
                     LNNVCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKSNTHRRMKYARQIYSAYMQG
                     R"
     gene            34999..35625
                     /locus_tag="ACH3L1_000040"
     CDS             34999..35625
                     /locus_tag="ACH3L1_000040"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFT4074975.1"
                     /translation="MLQIIPALLVAITVVIQCLAVQMSRYRLSRMNARPPHHGLALMS
                     GMLAAAAIILRLYQNDSAVGVLFMTIMTGCMTLMTLTDAASCRLPRPFTIMYLISGSA
                     FRLWQGDWLTGLCSAGFWFLLLLLFRQYTSYRRKTETIGLGDVFLIAGIAIWSPPGDI
                     PWIVATAAGGALLYLWCNRQRRITRELPFGPFLCASQYAFTFYPGGLW"
     gene            35643..37004
                     /gene="pilV"
                     /locus_tag="ACH3L1_000041"
     CDS             35643..37004
                     /gene="pilV"
                     /locus_tag="ACH3L1_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFT4074976.1"
                     /translation="MQKDNDKGFALLEILGGLVVISLLMPLFWSYIEDYLNEMRNQSA
                     AFHADAYNTAARTYIADNNARLHSGTLPATFTADELIRKGYLKGLNRSPFGQSYTTGI
                     RRNTSTGRLEALTCSTGGENIKDDALRSIASLLPGLGGFIGKNGTATGVFGGWTDKPG
                     DYGLSCNGGHIAIVMMGDDLQESDRLYRFQVPGRPELNQMNTAINMGGNNLNNAGNIN
                     GQSATLKGDVTSENGWLITKNDKGWKNITYGGGFTMTDSQWIRAVGGKGIITTGEIKG
                     GKVSGGTVRSDGRLSTGEYLQLDKTAVANTKCSPDGLVGRDSKGAILSCQSGVWVGFE
                     SYPVGSPIPWPSATPPQGYLLMNGQSFSCSRYPQLARAYPSCKLPDLRGVFIRGWDNG
                     KGLDGDRNRQLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYIV
                     KAS"
     gene            37118..37312
                     /locus_tag="ACH3L1_000042"
                     /pseudo
     CDS             37118..37312
                     /locus_tag="ACH3L1_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            37477..38298
                     /locus_tag="ACH3L1_000043"
     CDS             37477..38298
                     /locus_tag="ACH3L1_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFT4074977.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSHRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PATQEIFCRIISARENMDIILWHGFPFAGTHSGEYCGVLLRQKKEINNTIIYPVLSLY
                     PARMDIEKEGWFQMARKISPDIPVRAVTLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            38400..39602
                     /locus_tag="ACH3L1_000044"
     CDS             38400..39602
                     /locus_tag="ACH3L1_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFT4074978.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIADNADKISDDWKAFDRAVD
                     SHSGVPQTAARLKERLQDFRNTHASAQAGVSAVAALPGDTLAAALMLKTLGTVSVDGK
                     VSDADLNYLESIADSGSQDVDKNRLTSQAFARAALITDVGVALATELETAGQKWSLGF
                     TPKFQRVDLFNYNTLIKNYDSSAFKGDRYHNTQNGINADIGASMDLDDNWTLGLVAQN
                     LIPRSIETKEVHGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AVGAEFNAWKWAQLRTGYRQNLASNNGSAFTAGIGLSPFDVIHIDVAGLVGTDNNYGA
                     IAQLQFTF"
     gene            39706..40164
                     /locus_tag="ACH3L1_000045"
     CDS             39706..40164
                     /locus_tag="ACH3L1_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238171.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFT4074979.1"
                     /translation="MQKKRITLAGGVAMLCLSGCHLPPDNTAPARKYGAASEVAVTRH
                     AQYQLWEQGTYSAALPSRQPGGSLQAHSGRVSFDWEGDALELLSELARLRGQQFSYSG
                     VRLPLPVSLHVRDMTFADALRLTEAQTAWRAVLSQSAGRLHLSYMPTEKK"
     gene            40161..40997
                     /locus_tag="ACH3L1_000046"
     CDS             40161..40997
                     /locus_tag="ACH3L1_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFT4074980.1"
                     /translation="MKKQVCLLLSGLLLAGCAGQHHRPPAGMAGNLPPPDLRAWLTPT
                     DNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISR
                     EGWLPPVIDEAQDVAHIQPDQIRTASRVWTILRPERFVSNPPGWRDWLLRGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRDNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQRQTVTGNGRKLITGDHVRRLKTHAEFTLQKSRWRPVVSTEGAPDEI
                     TR"
     gene            40981..42129
                     /gene="traJ"
                     /locus_tag="ACH3L1_000047"
     CDS             40981..42129
                     /gene="traJ"
                     /locus_tag="ACH3L1_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFT4074981.1"
                     /translation="MKLPDEFGTFPFARFTAEEFRYFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSAHPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKVISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRYIQTHFPDRKTVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRDTLSRQS
                     HATWGHYIDEIIRKEKRRIRDQVLTMYQTGSIEASEAALFIPAGEFPK"
     gene            42126..42416
                     /gene="icmT"
                     /locus_tag="ACH3L1_000048"
     CDS             42126..42416
                     /gene="icmT"
                     /locus_tag="ACH3L1_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IcmT/TraK family protein"
                     /protein_id="HFT4074982.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFVLLGYSGWTVRTLCRRLYSLVQGKQASGRPWWYRHRTELPREWRGTE"
     gene            42480..46541
                     /locus_tag="ACH3L1_000049"
     CDS             42480..46541
                     /locus_tag="ACH3L1_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFT4074983.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYRAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQAGYARSYINGLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDASNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDNFQNAMNAQLKHIGIPTPNSDTADHREA
                     VVIDNLIFTPVKDEKPQMSPENRQSTAPETELATQDTPYDTHSDDTENQAYEDDYAAY
                     ASLQSVTAETLSPDKPLENIASAADTGTENTSVPTAGDQEDLNTDATGTDTAHNSDTA
                     ETGTLPEPAGMQSVQQATTEPTAEDKPHENVVSAADTREENASVATADTGEENASATT
                     ADNQSTQAEEHPRSEDTPETSLSDAAGTQHTTTTQSEEQVQVSMASADDWSAFAEDLN
                     ADTPGAPTVHNTDNAETNTVSEPAGMRPVQQVTTELTAEDKAQEKSVASAAETGAEHS
                     SDITDDNRNAPAEELNADATVAVHNTGTAETASPSEPAETQPVRQATAEPTAEDKLQE
                     ERLVSATETSTANEAARPETPSLFSRLRRFLTGVKSRTSEPSVIQEKKPTEAETATEE
                     VAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSV
                     DGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIV
                     EYDIKVRMKLPAQRAALEKLRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVS
                     PKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEHPAAPSSAPEKAPVSAQPQQPDT
                     PVEEPPGKLSPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVK
                     NHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFP
                     MATFSQVMLQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAP
                     NELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINAL
                     TDNGLQFAGYGEAVNHDENNHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSL
                     GFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGR"
     gene            46558..46908
                     /locus_tag="ACH3L1_000050"
     CDS             46558..46908
                     /locus_tag="ACH3L1_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFT4074984.1"
                     /translation="MNMKKTLLVILTGITLASPVAGAATCRAGAAAEAGSRAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDISITLPQFPSLDKLLRDLENQVCDAVVDRVND
                     HLPGNIDPWKDYNL"
     gene            46920..47615
                     /locus_tag="ACH3L1_000051"
     CDS             46920..47615
                     /locus_tag="ACH3L1_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFT4074985.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLYSGAALCISLVANGIQYWHATGVTREYFATDNGRLVRLAPTSQPAWSQSDVMNFGS
                     MTLSEAFNLDFVHYRKQITTQAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTTTIG
                     AGTLVNQGQMENGVWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAAAGI"
     gene            47626..48600
                     /locus_tag="ACH3L1_000052"
     CDS             47626..48600
                     /locus_tag="ACH3L1_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFT4074986.1"
                     /translation="MHIHHLIGALLIVCGTVTAADNPGWKNARSPQTGGKTAQATGNT
                     PAGGPPPAAQLPGNLPPPGAALVQEGASLDSPLNASEIRELRGIMADNERAISAPLTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDVNGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTTTLSQEIDSRLDLRIPR
                     QGPASPVVSIPSEKIALHDATLQAFLDGVPPDDPAIRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            48604..49941
                     /gene="traO"
                     /locus_tag="ACH3L1_000053"
     CDS             48604..49941
                     /gene="traO"
                     /locus_tag="ACH3L1_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFT4074987.1"
                     /translation="MSAEQEQDTAAKSGKKLSALLVMGGVIIIGGGYLLLTWLSPENA
                     ASLSSVNINSTTSGGGRNVMETQHYRDLLRADNERGAAEALRNNETFIASLPRGLDTD
                     TGNDQKRDNPPDDQSGTSGQNASPPQEDQTAREKRREALQKLLARINTQHQDARPPVV
                     AAAMWSTTGTPGTAPHASPPGTVTSQHPRLQETSLATPAARPGIQLIPALTRVPAYID
                     TEVDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGQGMDIHFDRMMWRGMFLKVNAYA
                     LNERTGMSTVASDVNHRWFKHIVLPSVLGGVGNVGSLYKDANTQILQGNYGSITGRVG
                     MPDGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDIST
                     PSSGTAPSESPSSGRPLTQAEAEHQASDRIRAAVLRKQNEMQQRYGIQQESYP"
     gene            49938..50651
                     /gene="traP"
                     /locus_tag="ACH3L1_000054"
     CDS             49938..50651
                     /gene="traP"
                     /locus_tag="ACH3L1_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFT4074988.1"
                     /translation="MKNEHEDPGSFGETTAPASGKSFLKRQYMGYSLPVWAGCALIIL
                     VAIWYKFLRTPSEPPADAGFAVTPPAAESWAEPMTMTESGSGNNMPAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKDSITTLSNAIQKDEAWAQETRQLLEETRKQVHTL
                     QMQGALPAPTGNNRQPAKTLPSPLQGMKIMSVEKGMAWIRWQGSAWAVREGDSLGNIT
                     ITRIDPSSRAVITSAGTLR"
     gene            50648..51178
                     /gene="traQ"
                     /locus_tag="ACH3L1_000055"
     CDS             50648..51178
                     /gene="traQ"
                     /locus_tag="ACH3L1_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFT4074989.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIFICGMLVSLPSMINAGGTQMGFRADSFAPIAYVQPGSFGAA
                     AGAVNAILSLAKLAGVGFVMNGISIWRKSLLDGHTALSASESISKGNVKFVAGVLLVF
                     NDRVIDATLASLGIAF"
     gene            51225..51623
                     /locus_tag="ACH3L1_000056"
     CDS             51225..51623
                     /locus_tag="ACH3L1_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFT4074990.1"
                     /translation="MSRLRAMAARLRAASVYVQLCLMACRDRLVRLLLLLPVMLLPHT
                     AMANTLFDMIKTVGDGARGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGVCLAVVPELMGSGQKTLGLSPVSIS"
     gene            51680..51931
                     /locus_tag="ACH3L1_000057"
     CDS             51680..51931
                     /locus_tag="ACH3L1_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFT4074991.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKAYEKDPATHRPGPGTMAFPAPATGHGIQR"
     gene            51846..52664
                     /locus_tag="ACH3L1_000058"
     CDS             51846..52664
                     /locus_tag="ACH3L1_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFT4074992.1"
                     /translation="MKKTPPLIAPVRERWLFPLPQPDTVFSGEALLPPPVLSTEGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTIVLLEKNLTRVMRLKKRPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESDLLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALQEIFSDNRQRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            52959..56003
                     /locus_tag="ACH3L1_000059"
     CDS             52959..56003
                     /locus_tag="ACH3L1_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFT4074993.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDQTAPGTLESLIAALAERMNTAW
                     KNSGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKNHQTRVRKLAEHAPPARFAQDPWRWTLSALKIRHDALLD
                     TLEQALTHDSDGLLIRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGGD
                     TSVFHAPSLNLQLFTTQPETHGSLIQAGELWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPDGMKALGVKKTLLTYSSFIPPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PDHTRNLFDVMYGARKPVTPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISSLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHIAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSEERLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQHYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYNL"
     gene            56003..56623
                     /locus_tag="ACH3L1_000060"
     CDS             56003..56623
                     /locus_tag="ACH3L1_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFT4074994.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESLSHRVPDILYPPKTEKKECKDNLQPHCLHSL"
     gene            56581..57786
                     /gene="traW"
                     /locus_tag="ACH3L1_000061"
     CDS             56581..57786
                     /gene="traW"
                     /locus_tag="ACH3L1_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFT4074995.1"
                     /translation="MQRQSATTLLALLVGCAVIPATHAYSVNVVSSQPVTRQIMPELA
                     TIQGIMEEMVRMQTATGTAITQNSEKLATVIAQDGQATRQQMIFSNETHRLEEARKSF
                     SVPDSICSESASGIAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPVREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGDLPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            57783..58352
                     /gene="traX"
                     /locus_tag="ACH3L1_000062"
     CDS             57783..58352
                     /gene="traX"
                     /locus_tag="ACH3L1_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFT4074996.1"
                     /translation="MKDDNSIDEKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     DQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVDELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPVITLIRILVCLLLMSTIAALCASKALIASYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            58427..60592
                     /locus_tag="ACH3L1_000063"
     CDS             58427..60592
                     /locus_tag="ACH3L1_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFT4074997.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIATMGIVWFLFIGIRHAVRTGHQGSVFNN
                     GRDVVGILSVVSGFLMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMELCKHAMNTGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNKQSSLAHFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPADNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLEDLANRAPTVSGVSSLGEAGDTEFLKEVKGAYQ
                     AQLQNSAYTPALGTATSASEYHASKTQDPQSAILKLIGFNLTRMTNWTATYLSGTGTT
                     TNQLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALYAAALM
                     NVQINSVTGVFSMAGFLMIYARACTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNMRRTPGVREEKLKNINRDDKDGIKQ"
     gene            60680..61333
                     /gene="excA"
                     /locus_tag="ACH3L1_000064"
     CDS             60680..61333
                     /gene="excA"
                     /locus_tag="ACH3L1_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFT4074998.1"
                     /translation="MNTTKSRYPSWWAYYYLIRTAYFIFGVPFLLLCSLGFTGMMLTE
                     PYIETDIQAYLYTYGSWLLLILPSLWLYMKTRAKKKKVQQVVQQIKESSPFAPTSDYE
                     QLSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVS
                     LPMIPLEISGISARTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            61607..63193
                     /locus_tag="ACH3L1_000065"
     CDS             61607..63193
                     /locus_tag="ACH3L1_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFT4074999.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNSIINNLISLSTAKTIAH
                     DKVEIQKNSFKQNTPLPLCQPNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMR
                     LGTDDSPHSLADIAVKLGISRERVRQLQERALGLLAAKSRYEGTPGACLKALINSIGN
                     SPYDIAVWIYRIVHRDFVTSSDLAAKFIIFTAGIPKARRDEIKSSLSLISQLQKQRLR
                     ERRAELAHARAQERARIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTD
                     ISGFFYSQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPD
                     LLIATVDGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEE
                     HAISTNTWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDNFYRIT
                     PKISNNTSNQRQPNESDCHN"
     gene            complement(63331..63483)
                     /locus_tag="ACH3L1_000066"
     CDS             complement(63331..63483)
                     /locus_tag="ACH3L1_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFT4075000.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRIEQGNTVLVATL
                     AYEVKR"
     gene            63813..64055
                     /locus_tag="ACH3L1_000067"
     CDS             63813..64055
                     /locus_tag="ACH3L1_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075001.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     MTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            64354..64722
                     /locus_tag="ACH3L1_000068"
     CDS             64354..64722
                     /locus_tag="ACH3L1_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075002.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQHSHEQHIEELRAALARMLHMHHMMLKTTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            64726..64941
                     /locus_tag="ACH3L1_000069"
     CDS             64726..64941
                     /locus_tag="ACH3L1_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075003.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(65139..65762)
                     /locus_tag="ACH3L1_000070"
     CDS             complement(65139..65762)
                     /locus_tag="ACH3L1_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4075004.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMRECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRVDSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRGVSGYSL"
     gene            65922..66389
                     /locus_tag="ACH3L1_000071"
     CDS             65922..66389
                     /locus_tag="ACH3L1_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFT4075005.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSASQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            66540..66716
                     /locus_tag="ACH3L1_000072"
     CDS             66540..66716
                     /locus_tag="ACH3L1_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075006.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYMSWGVPGKSRLMFDSR
                     EQAQEVQEGFRFLT"
     gene            complement(66776..66874)
                     /gene="dqlB"
                     /locus_tag="ACH3L1_000073"
     CDS             complement(66776..66874)
                     /gene="dqlB"
                     /locus_tag="ACH3L1_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFT4075007.1"
                     /translation="MINLLIIVLRAVVAVANAVIALLELIRGLVDL"
     gene            67671..69014
                     /locus_tag="ACH3L1_000074"
     CDS             67671..69014
                     /locus_tag="ACH3L1_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFT4075008.1"
                     /translation="MSYQHSPTTEDPMNIWYIVGTICLLFALLIWLFLPDIVFATCLI
                     LHTMWGMTDWGPFHHFAAPRYNLLANTANNAATITFSQWLDVMSQTVGILWLILLPMT
                     IGFLWMWFQHPAQPRFTRRPLNIHTLPHLFSSLSPAIAPVLADGDSNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVAATRQCFMAQLGQPLTTWKDMAPHEKALFAVFGLQ
                     FFLGDRKSAVSLMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEDAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTETTETTETTETTETTETTETTEKQHPADKEKEQ"
     gene            69011..70135
                     /locus_tag="ACH3L1_000075"
     CDS             69011..70135
                     /locus_tag="ACH3L1_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFT4075009.1"
                     /translation="MTIHYTATLNNKHLSLTALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHTGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            70116..72419
                     /gene="trbC"
                     /locus_tag="ACH3L1_000076"
     CDS             70116..72419
                     /gene="trbC"
                     /locus_tag="ACH3L1_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFT4075010.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            73283..74098
                     /gene="sul2"
                     /locus_tag="ACH3L1_000077"
     CDS             73283..74098
                     /gene="sul2"
                     /locus_tag="ACH3L1_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFT4075011.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            74185..74487
                     /locus_tag="ACH3L1_000078"
     CDS             74185..74487
                     /locus_tag="ACH3L1_000078"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075012.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
BASE COUNT        18731 a        20258 c        18690 g        17098 t
ORIGIN      
        1 ccggacgcgg acactgacca caggcagcgt ttatgccgcg ccgcgtcagc cggcgcagta
       61 aaaagaggag cacggcgaca tcctttaccg gaaaagaaac gggcggttgc ccgcccgtct
      121 ccgtcatact tcatccgcca taatgcgcca cattgtcgat tatccacgca ggtccggtga
      181 cttccggcag ctcatcatca tctgtcgggg aagaccattc cagttccccc cacagcacat
      241 caacgagctc tccgcgctca tacaactgcc agccacagaa attgcagccc tgttcggcgt
      301 accagtgctc cagcgtgcag ccgaaacggc ggcttaattc tgcaacaacg tcgctttccg
      361 gctgacacca cggcgtgtcg aaatctacct gaatgaagtt ttccccctga ctgctgatat
      421 tcaggtcata cccacaaggc cacttcacgc cataccgttc tgtataccag tgatatgcag
      481 aaggaacacc gttcagaaca ccgccgttaa agccattcac ttcaatatcc aggcgggtcg
      541 gcaaaaccgt cagcaggtca aacggcatgg ttttttccgg cagtacgttg tcacacagac
      601 ggttccacca caccgataca tcctcgttac cccagatgtc acacctgtca cctctttttg
      661 cggtgaaatg cacagtgata gtttccctga catcatccgg taactcttcc cagcgacgct
      721 gtccagtacc gctctgcagc cagagttcat gcagcatcag gcagttttgc tcatccagca
      781 gtacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc gatattttcc ggtgatacag
      841 caccacgtcc ggcagccgtc actccggggc actgctcaga gcggatattt tcagtgatct
      901 gcagaagtcc ggcacttccg gccagaaaca gctgaatacc ttcatttgtg gcgcggcgat
      961 aaagcggtgt aactgcaccg ctggccagtc gtttaatctc agcgatctgt gccggttcac
     1021 cagaaaaata catacgattc gagcaccagt ttggcatggg aattctcctc tcacatgcgg
     1081 cctccatcag gccgtgccgc acctgttacc gggcgaccgc atacgaatgc aggtgcggcc
     1141 ccgaagccgc agcgcgtgga ggaggaacga cgaaacgtga ccgggtgagg aggccggcga
     1201 cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc cggcacggcc tgggtaacca
     1261 ggtattttgt ccacagaacc gtgcacaaaa tgctgtggat aagctggaca cagcagccca
     1321 cagcaggcat acagccacac actcagagaa ttgagcgttt tttagtcaca acaatgacat
     1381 ttgactgtgt gcggattcag ttattagaat actgtataaa catacagcat aaagaaaagg
     1441 gagatgagaa catgatccgc attgaaattc ttttcgaccg ccagagcaca aaaaacctca
     1501 aatcaggcac acttcaggcg ctacagaatg aaatcgaaca acgtctgaaa ccacactacc
     1561 cggaaatctg gctgcgtata gaccagggca gcgccccgtc tgtatctgtc acaggagccc
     1621 gcaacgacaa ggataaagaa cgtatcctgt ctctgctgga agaaatctgg caggacgaca
     1681 gctggctgcc tgcagcatga gtaccgttta tcaccgtccg gctgaccctt caggcgatga
     1741 cagttacgtg cgaccgttgt ttgccgatcg ttgccaggcc ggttttcctt cgcctgccac
     1801 tgattatgct gagcaggaac tggatctgaa cagctattgc atcagcagac ctgcagccac
     1861 cttctttctg cgcgccagcg gtgaatcgat gaaccaggct ggcgtgcaga atggtgatct
     1921 gctggtagtg gacagggccg aaaaaccaca acacggggac atcgttatcg ctgagatcga
     1981 cggtgagttc accgtcaaac gactgctgtt gcgcccacgc ccggcactgg agccggtttc
     2041 agacagcccg gagttccgca cactgtatcc ggaaaacatc tgtatttttg gtgttgtcac
     2101 tcacgtgata cacaggacgc gggagttacg ctgatgtttg cactggctga tatcaacagt
     2161 ttctacgcct catgtgaaaa agttttccgc ccggaccttc gcaacgaacc ggtcatcgta
     2221 ctcagcaata acgatggctg tgtgatcgcg cgcagcccgg aggcaaaagc ccttggcatc
     2281 agaatggggc agccctggtt tcaggtgaga caaatgcgcc tggagaagaa aatacatgta
     2341 ttttccagca attatgcgct gtaccacagc atgagccaac gggttatggc tgttctggag
     2401 tcgctttctc ccgcagttga gccctactca attgatgaaa tgttcattga tttgcggggg
     2461 ataaatcatt gcatctctcc ggagattttt ggtcatcagc tcagggaaca ggtaaagagc
     2521 tggacaggac tcaccatggg ggtgggcatt gcgcctacaa aaacgctggc taaaagtgcc
     2581 cagtgggcaa caaagcaatg gccacagttt tccggtgtgg tcgcgctgac ggcagaaaac
     2641 cgtaatcgga tcttgaagct actggggttg cagccagttg gtgaggtctg gggagtagga
     2701 cgcagactga cggaaaagct gaatgcgctg ggtattaaca cagcactgca gctggcgcag
     2761 gctaacacgg cattcatccg gaaaaacttc agcgtcattc ttgagcgtac ggtacgcgaa
     2821 ctcaacggcg agtcctgcat atctctggaa gaagcaccgc cggcaaaaca gcagattgtc
     2881 tgtagtcgca gttttggtga acgaatcaca gacaaagatg ccatgcacca ggctgttgtt
     2941 cagtatgcag agcgggccgc agagaaacta cgtggggagc gtcagtattg ccggcaggtg
     3001 acgacatttg tacggacatc cccctttgca gtaaaagaac cctgttacag caatgccgct
     3061 gtggaaaagc ttccattgcc cacacaggac agccgggaca ttattgccgc agcatgcaga
     3121 gcgttaaacc atgtctggcg tgaagggtac cgctatatga aggcaggtgt catgctggct
     3181 gatttcacac catcgggtat agcgcagccg ggattatttg atgaaatcca gccccgtaaa
     3241 aacagtgaaa agttaatgaa aacactcgat gaactgaacc agtcgggaaa agggaaagtg
     3301 tggtttgcgg ggcgaggaac cgcccctgaa tggcaaatga aacgggaaat gctttctcct
     3361 gcgtatacca ccagatggac agatttgcca gtagcccagt tatgaatatg taatccaccc
     3421 ataatattgt tatcctgtta tcctgctatc ttgttatctt gttatcttgt tatcttgtta
     3481 tcttgttatc ttaattaatc tataaatcaa atacttaaca acttgctaag ttgttaacat
     3541 aacaaaaata attgcatggt tcgcgttcag atggcaatat taagttgtta acttaacagc
     3601 ttaatatgag gctataatga taatagtatt aggtagtcaa aagggtgggg taggtaaaag
     3661 tacactggcg gtatctatag ctgcgtatct gatgtcacta ggcaatagag tgattattgt
     3721 cgacgcagat gaccagaaat cagtattaac ctggtacaac aatcgccctg aagatctccc
     3781 tcatattccg gttacaggcg cgacaggtaa catcaaagcc atgttaaagg aacacgaaaa
     3841 atcctatgat tttgttattg cagactgtgc tggccgtgat agcgcagaaa tgcgtagtgg
     3901 attgatggcc gcagatgtat tcatttctcc tttgcgccca tctcagatgg acctggatgt
     3961 tgtgcctcat acatgctccg tttttacagc agcaaaagat tttaatgagg atgttcgggg
     4021 atatctcgtt ttaaacatga cgcccacaaa tatgtttgtt aatgaagcca atgaggcagc
     4081 tgaagtactt aaggattacc ctgaaatgca tctggcaaac tcccgggtat gtgatcgtaa
     4141 agcacatcga gatgcttggg ccgaatcaat gacaattttt gaaacgcaaa atgataaagc
     4201 ccagcaagaa atcgaagcgt tagtgaaaga ggttattctg tgagaaatag agcaaacact
     4261 tcacctcgtg acgaggcagc atttattaac ggtggcacgg ctggccttga taataccaaa
     4321 aagtcatcag ataaatcatc ggttgtaagc aaagcaaaac cagtgagtat ttcttttttt
     4381 gaagaaaacc taaaagacat tgatgccctt ataacggctg agatggtttc aggtaaaggc
     4441 agagtgaatc gttctgatgt tgtcagggcg gcggtagaag cacttaaaac actatcaaaa
     4501 acagaggtat cacgattgat agcagaagta aaacaaagat aacaccttgt tatcttgtta
     4561 tcttgttatc ttgttatctt gttatcttca atgtatttta ggatgaccta tgttgaatag
     4621 aacctaaaac aaaacaaata ttaatagggg tccgcttgga aaagggaaat tttcccacgc
     4681 taagagaatt gagttgaacg agtggttaca tccaggccag ctaactcact gaacgtaaag
     4741 gctgtacgaa atgttccggc cgtccgataa ccgtttggct tacttccgta cattcccttg
     4801 cgtagatgta cagaattgta cgatttcgga caccaaaacc gctgttgtcc cgccattctg
     4861 gcgattcctg aaagtccttt gactaaaaaa tagtcttaac gtggtttatt ttccgaattc
     4921 caagcggacc cctatcaggc ctgccgtggg gattttgtgg tgcgtctcga cggcagcacc
     4981 tgtctgcagt tatggaataa agaaggcagg gtggtacgcc gggagggcga tccgctggaa
     5041 gtggcgcagt ggctgctggc ctgtcatgac gcaggaatag aagttcgcgt acagataaat
     5101 gaaagtgccg ctccgtaaga acacgcctgt gcacggttta cacatcatcc catggtggca
     5161 gccggttaac tttcctgcgc caccagtgga aagaggccat gcacagtccg aacaaaaaac
     5221 cccccgtgat acttataata atggcctcac ctgccaccat tcctgacggc ccccagtaga
     5281 taaaccacat tgcacatccc caggaaatgc cccataagcc ccccgtcagc accgtgacct
     5341 gccagaacgg cataaagggt aatggcggaa gccggatacc cagccgccat agaatacgca
     5401 gaagaggagg ggcataattg ctccgccaca tctttttgct gtccatcagg gcaatggccc
     5461 gggctttttt ctgctcaaag gtcacaggag cacctccgtt ccatgatgta aacgtgacac
     5521 agcttattgt tattgattat tattatcatg ctgtggttat gttgtcatgc atcagtacac
     5581 tgacaaaagt gcaaaagaaa ggaaaacagg cagatgaaca gaaaatatta ttttaataat
     5641 atgtggtggg gatgggtgac ggggggatat atgctgtata tgtcatggga ttatgatttt
     5701 aaatacagat tactgttctg gtgtatttct ctctgcggaa tggttttgta tccagttgca
     5761 aaatggtata ttgaggatac agctctgaaa tttacccggc ctgatttctg gaacagcggt
     5821 ttttttgctg atacacctgg aaaaatggga ttgcttgctg tttatacggg tactgttttc
     5881 atattatctc ttccgttaag tatgatatat actctttctg ttattataaa aaggctgtct
     5941 gtaagataga cagcctgtct tatattttat ttaaataccc cagaacttat tcgctttttc
     6001 cacaagcgat tcatcaatca gcgcaccggt gacagccatc agtaaaccat acccgataat
     6061 gcctaaagcg cttccggtaa gaatactgaa gaccagtgca acaagagccg ttgcggcatt
     6121 gcctgctata atggcttctg ttttaacaaa aagaggacgc cagttctctg tccggacagc
     6181 cttaccaaac tcagtgatcc agtcagcaag acttgtaaat tttcctgcat atcccagtcc
     6241 ccgactgaat ctgttcagat tagacgatat atcagacagc ttcacagact caagggctgc
     6301 ggcaatcgct gcacgatctt ttgcattaat ttttttgtta atgtcagccc ggtacttttc
     6361 atacgttttt aatgcctctt caacattacg tattttcttc cctttagcct gcccggccat
     6421 ctctctggct aactgctcag cttttgcacc atatttttct gaaactgatt tcaggaactc
     6481 tgttgtgaaa ttaattgcgt ctttcgtttc cttcagttca tcctgttttc ttttctcttc
     6541 cgctatcttc tgattgatgc cggcaagctg gttacggata ttctcttttt ccttcaacag
     6601 ggcattaaga gcgtcatttg catgcttttg ctcatttgtt ctggcactga ggttatttct
     6661 cgccgaattt accgcagatt cagcagaggt gattttattt ctggcatcaa gcaatctttg
     6721 ccgtaactta tcccattcag caacttcagc attataaatt ttcttgtcac cttccacatc
     6781 ctctgtcacc accggattcc ggtcattcag aatattgcgt ttatagtcca gaccggaatg
     6841 ggtcagaaga taattcaggt ttgcacggtt atcgatgact gcgctggttg tgatttttat
     6901 cgtaccggca aatctcggat caccgctcac aacaatagaa tgatttgttg atgaacgccc
     6961 cgggaatttt tcaggggaaa ctatcgtttt gccgtcaggc gtgtttttct gttgcgtcag
     7021 ctgttgttca gctctggtta atgcattacg ggccgtgtta agcgattccg tcactgaact
     7081 gagtttattc cgggcctctg aaatggccct gtcattccgt gtctgctgcc cgagcagacg
     7141 ggcctgttgt gcatcaagaa cggcaagggc tgccccgcca tttttttcca gctctgacaa
     7201 ccgcgtgtcc agaatacctt ttctgcgctc gtattcagca agcatatcag cggccttctt
     7261 gtctgcgttc tgcgcatcct tctgccgttt ttcagcctgt tcgattaacg acctggcctc
     7321 ccggggagat atacgaagag aatcagcatc agcaaacaac atccggctct ctgtatgcag
     7381 ccggagggca tcatatccgg cgatttcagt acggaatccg tattctctca gctctgcctg
     7441 caggagtctg aactcctgtc gtgtaatatc agcggcatct gcgtttccgg cacggagtgt
     7501 tttcagtgtg ttttcatctt tttcccttac agcaatcgcc gcagagaggc gttttcctgc
     7561 ttcagtacgc ttattctcat tctctttctg ctgcgcgctg agcgtgtttt tgtacgctgt
     7621 gatttccttg tccctttttt cgatatcact tcgggttggg gaatcatcaa cccactcgtt
     7681 tccgccccag atggttttgt tcccgaagga actccatgca ggcggggtac catgaaagac
     7741 atcgacacgt ggagggttta cataaatatc aacggccata atttcatggc catctgaatc
     7801 ataccccagc gattctgcac cgggatttgt aatacgtaca gggtcagaca ttcggtatct
     7861 ccttcatcct ctgaggaaaa caaatgaatt aactgcttac gcatatggat acatatacag
     7921 tattaattta ttaaggagaa aatggcatgt caagatttaa tgatagtgat tatcattaac
     7981 atgaagtgat gttacaccgt tcagaaagcc tgctgacact gtggcagcat cacagtctgg
     8041 gtattctctg gtgcaataca aaaaccggac tttcccgtaa caacagacaa tgttacggga
     8101 aaagttacag aactgagcca caaaatatct cagtacctga gatgcataaa aacaccgtac
     8161 atatgtttct taaaaggggg atgatcgtta tccagccatt tcggtggatc ggtatgcaga
     8221 acaccaatct gcttcatgcc ggtatgctgc ctggattttc tcgcgcagta atcactgtct
     8281 tcatatccca tcgtttcgat gaccactctg gccgtctttc cgtcaggagc ccttgctgta
     8341 acaatgaaat ccggcagtac atatttcttt tcaccattaa cataaacttc tgtatcaaac
     8401 aggggccttt ccagacttaa ttccggcgta cctggctttc ttttaagcca gctggccaca
     8461 ttcagaagag cagtgagcgt gtttcgttcc agtttgctgt ccacaggcac cggacatgtc
     8521 agttgataaa gagcatgagc atacccttca ctgcagatga ttctgccatc ggcactccgg
     8581 caaaaagcaa gaataaccca gtaaggggga cgtattccgc cctgcgcttc accattaata
     8641 ctgatccccc gcacaggtct gatgacctga atattaccgt tcgcccagtg aatttccaca
     8701 gcatcacgac agatgtgctg cgccataaaa atctgaaaga acatatgctt tcttctggca
     8761 ggccagtgtc gttctgaatt ctccagcgtt tccattaacc gctcctgaga cctttcagac
     8821 attcctggcc ggaaatggat aatttcagat aaggctcttc cccgaatata ctcctgctgc
     8881 agtgtaacct gccgtatggc atcaagacac tcacgataat tagtccggat acggggatta
     8941 gccagggaat ccagtttatt cagccctgca tcttcaataa gcgacaggag taaacggcca
     9001 aggcgcggac gtttcttgcg aacggaggag cgctcaccat tatggtaagc agagcgggcc
     9061 tgcagtctga taacagaatt actgtcatca ggtggtaaaa agtttctgta atttatacgg
     9121 gtgctaacgg gatgccggct tgctccgaag gatgtttcct cttcatttcc ggataactga
     9181 cgatacatgg ggcattcagg tgcatgctca tgattaaaac tggacagata aagagtaccc
     9241 gtattgtttt taagagcagc aaaattcatg gccggcgaat ctcccggaat acagtcacac
     9301 tgtaaccatg catcatactc acgcatttcc cggagaatat cattggccag gttatgtagt
     9361 gccatggttg ctggcctttt cagtccacag accgtcttta ctgcagtctc ttcctcactg
     9421 ctaagctccc gaatcacttt gccccgggga ttcttcttta caagtatcat tggcataatg
     9481 aaagcactcc ttatgtacat gcttaaacag taagtaaaat atacaaagaa caccccatga
     9541 aacaaaggca gagatgagct gtgcgccccg atatcttcta ctgatatcct ttcaaatgaa
     9601 ttgtttttat ttatatcatg acaacattaa agtgagagaa tatatcaacc atgtttctgt
     9661 cagggttcag tagaatatca attcgactgt cacgccataa ctgttactac ctcgagtata
     9721 aatatccaga gaacggactt attagccatg aaaaatcacc tgatggaaaa tttactaatt
     9781 tatgaattgt gatattatta aagagtaaaa ccagccagcc gaggtattta tgggaaaaaa
     9841 tcaggaagaa aggaaaactc ccgtcattgt ggtgaaaaaa cgtcgtactt tttcgctacc
     9901 gtccttatcc gaaaaaacag atattatcgc acctgtattt actgaacaga cggcagaatc
     9961 agcccccgcc ggaataaatt catctgccgt cgaaacacat atccccgaag cggcagccag
    10021 gaagaagaaa aagaaaagac accgttttcc acgtccctcc cactggaccc gtgagtatac
    10081 tcacgagtgc gtggaaaaaa taaaggccct gtttcctcat cttcgtgctg aaggtggtgg
    10141 atttattccc ctgaaaattg gcataaacaa tgatatttcg gcatttctgg cagaacaccc
    10201 ggagacagaa ctgaccatgg acgaatggct ctgtgcagtg tcctgcatca catcccgccg
    10261 cgtatatctg cagcggacag ctgtcgccgg cgtcccacgt tatggcctgg atggtcaccc
    10321 caaagggcag gtctcagact ctgaagcaca gagtgccggt cgcagactgg cgacattaga
    10381 acaaaagtgg ctcaggatgc aggcacagca ggagaatatt tctgggcaat aaacactcct
    10441 gataacaggt ggacattacg tatcagaaaa aatgggaaga gaatgttatg tgtgattcag
    10501 aagcggagaa gattttattc atcctgaata aacatgaacg ggcatacaga tgccagtacc
    10561 ccgtatatat ttttttagct atagtattat ggattttacc tattctcctg attatatacc
    10621 tgcatttctg tataggaatc ggtttctgta ttgcatggac agcatactac tttctttcgc
    10681 ttttcgagta ttttgtaaaa tacttcgttc ctctcgatcg tgaagatttg cttaacctga
    10741 tggatctcgc caaagattct cctgatgcga aacaggagct tcttgagcgt cttttgtccg
    10801 gaaaaagaag accgggcggg atgaaatgga tatcaagcgc attctaaaaa caaaggaagc
    10861 acttgataac atcaggaatt ttattgataa acagcagtta acgaatagtg atactccgaa
    10921 gaaaagcacg gtcccaaatg aagataaaaa aggcataaga tgatgatcac aggaaatgga
    10981 ataaacactg taactgtcaa tggaaaagtg aaacacatca cagaactgga tgatataaca
    11041 ggggtcagtt tggatatcga aaattattgt acgttaaggt tgtttgtaag cgtgcagcaa
    11101 gaaccgtatt gacggggatg tgttattcag tcggcagtgc tacgcgccag gggagtagtt
    11161 cgccgacccg gtttatcggc cagtcggcta tgacgtcaag gacatagcgg aggtagcttt
    11221 ctggctccac tccgttcagt ttgcacgtcc cgatcaggct gtacagcagc gctccccgct
    11281 ctcctccatg atccgaaccg aagaacaggt agtttttgcg gcccagactg accatccgca
    11341 acgcattttc agcgatgtta ttgtccgcct cagcccagcc atcatctgca tagtacgtca
    11401 gcgccggcca ctggttcagg gcgtatgcga acgctttcgc cagttctgag tgtcgcgaca
    11461 gggttttcat cttttcacgc agccagcttt ccagggattt cagcagcggt ttcgttttca
    11521 actgacgttc ggcaaggcgc tgctccgccg tcattcccct tatctctgcc tcgatggcgt
    11581 acagttcgcc gatccgtttc agcgcttcct ccgtcagggc tgacggggtg cgaacgtgca
    11641 catcgtggat tttacggcgg gcgtgagccc aacaggcggc ttccgttatc cggccatccc
    11701 ggtacagctc gttgaacccg gcgtatgcat ccgcctgcag tacaccactg aaccccgcaa
    11761 gatggggctg cggatggatg ccttttctgt ccgggctgta agcgaaccac accgccggcg
    11821 ccagcgttga cccggcgtta cggtcgtcac gaacgtaggt ccataaccgc ccggtcttcg
    11881 ttttcttatt gcctggcaac agcaccggga caggcgtgtc atcagcatgg agcttaccgt
    11941 cagtcagcac atagtcctga agcgcttctt ccagcggtga cagtagccgg cagcatgcat
    12001 ccacccagcc cgacagcagt gaacgactca gctccacgcc ctggcggccg tacatttcag
    12061 actggcggta cagcggggtg tgctctgcat actttgagat cagcacgcgg gccagcagcc
    12121 ccggtcctgc gataccccgc tcgatgggcc gtgaaggcgc gggggcctgc acgatggcat
    12181 cgcactgagt acaggcatgc ttttcacgta cagtccggat aacccggaag gcgctgcgca
    12241 tcaactccag ctgttcggcg gcatcctcac ccagatagct cagcgagcct ccacattccg
    12301 ggcagcatga cgctgccggc agcagccgtt tttcatcgcg ggggagtgat tcggggaacg
    12361 gtttgcgggt gcgggtttga cgcagcgggc gctgcacggt cgggtcgtca acccgaccgg
    12421 taagggtatc actttctttc tgaagtgcct tcaggtcagc ttccatctgt gcgatacgac
    12481 gggagacttt ttcggagcgg ctgccgaagt tcatccggcg cagcttatcc agctgtgcct
    12541 gcagatggtc tatttcgcgt tcacgctcgt tcagcttttc cagcagggca cggttcagcg
    12601 cctcctgttc ggcaaggaga cgtttcagtg cattgatatc gtcaggaagt gagctgctca
    12661 taccgggtat attaccaggc tcattcagcg tcgaccagga taaagaggct tacaacatag
    12721 tcagggacgt aagcagtctt ttaggctgcc gccagtcgat atcttccagc agcatcgcca
    12781 gctgtgccgg tgtgaggaac actttgccat cccgggctga cggccaggcg aagcggccgc
    12841 gctccagccg tttggtcagc agacacagtc catcgccggt agaccagagg agctttacct
    12901 gactgccatt acgcccacgg aagataaaaa cgtgacctga catcggatcg tctttcagcg
    12961 tcgtctgcac ctttgccgcc aggccgttga agccgtttct catatcggtg atgccagcga
    13021 ccagccagat ctttgtccct gatggtaatg ggatcaacgt ttgagctccc gtatcagcag
    13081 gttcaggata ttttcgctga tggcaccatt cagacggagt gatccgtgcc ggaacgtcac
    13141 ttcacagctg atattgagtg attcaggtgt cgcagcagct acaggttgcg accgggggga
    13201 ggttgtaggg acaacatctt cggcatcaag tgttatcggg agcagctcag gcacgttgtt
    13261 ttctgttgtt gaaggaggac gtagttttcc ttcgcgccag tactggcgcc acttgaacag
    13321 caaattatcg ttgatcccat gctcacgagc gagttgcgct acggagatct ctggtcgatg
    13381 cgagagttca accattttga ttttgaactc aacgggataa ttagggcttt ttttacgcac
    13441 tgcggttaat gatttcatgg atagcgtcca ccatatttgg tgtccactat tctctcagga
    13501 atttcaggat ctgccagacg gggctgagac gacgcttacg gttgtttttt gaccttgtcg
    13561 atcttaactg atctcaaacg acgaaactta cctgaagtcg gtatccggtt gtttttccag
    13621 atataatcgc ctgtcagatt gatatgttcc caacctaatg gtgataaatg cgataatagt
    13681 tgagcattta ttttcatgcc cttacgcttc agagcatcaa ccgctctttc catgtaaact
    13741 gtgttccata aggaaatagc cgccgtcaat aaagtcaatc cactggcgcg ataactttga
    13801 ttttccggtt tccgatctct aatctccccc aatcgatgca tgaatacagc acgggccaga
    13861 gcattacggg cttctccttt gttcagcccg gcctggacac gtcgccgcaa tgccggatcc
    13921 cgaaaccagt ccagcataaa cagtgttcgt tctatacggc caatctctct cagagcttta
    13981 gccaacccat tttgtttggg gtaactggca agttttctca gcatcaggga tgctgtcact
    14041 gttccctgtt ttattgaggt cgccagacgt agcacttcac gccagtgaat ttctatttct
    14101 ttcagattaa tgcgggtcgg agatatcaga gaatgaagtg ccggatattg atccgcttta
    14161 cctttaatga aaagtttttt atcgtgaagg tcccgaatcc ggggacaaaa tgcaaaaccc
    14221 aacagatgca tcagagcaaa aacatgatcg gtgaaacccg ctgtatctgt gtaatgttcc
    14281 ctgatatcca ggtcgctttc gtggtacaac aatccatcaa gcacatgtgt tgaatctctg
    14341 acccgactga ttatgcaggt gtaaaacgga ctgtattgat ctgatatatg tgtataaaac
    14401 tggcatccag gctcttgccc atatttcggg ttaatctgcc cagcataacg accagaatta
    14461 ccagttctga agttctgccc atctgatgat gaagttgttc cgtctcccca gaatgctgcc
    14521 agaggtcttt ttccctgagc attcaccagc tctgcaaggg ctgcggaata agtttcatca
    14581 cggatatacc aggcctgaat atcttcgagt gatgatttag tacatccggg acaggcctct
    14641 gccattttcg tcaggccaag attgatgcca tcagcaagaa ttgtggtaag aagcaggcgt
    14701 gtatcgggac gagtcatatc attttttata tgtgaaaaat gtcgggtaaa cgctgtccag
    14761 ctgtttacct cgtcgagaat ttctgtgatt ttaggacgcg gtaacatact gtaaattaac
    14821 tctgaaagcg gtgagacctg caaaggaaca caattatcca gaggggagat ttttactcct
    14881 ttgtcagata tttccacatc cggcaattca ccgtgtgcgg ccattttgtt gacctcttcc
    14941 agttttgact gaagcagtat cattcgattt gtaatatatt catgataatc agcagaaaca
    15001 ggtagtggta atgcaggtga gagcttatca aagtctttct ctggaataag gtaatcgtcg
    15061 aaatttttgt aacgtcgtga gcctttaacc cagatatccc cggaacgtaa cgcccctttc
    15121 agctcactaa gtgcacaaaa ttcatagtat tgtcggtcaa taccagtggg agttatcacc
    15181 acttttcgcc agctttcagg aacaaactca agcggagctg tcgccgggac ttttcgtaac
    15241 tgtttcttgt acatatcttt gattacaagc aacgcatcag ccagcgcctg tgctgccggt
    15301 gtggcaacta attgcaaggt ggataacata cgcggagcat atttccgtaa tgtgctgtat
    15361 ttttcggtaa taatatgtag cgggtcaaaa ttattctttc tggcaagatg acgagtttct
    15421 tccaggctgg caataaattc aggccatggc aatattttct ctatagcaag ccatggatct
    15481 tcaccggaat catgggcctc agataaagcc tgtccgatat caatatactg ctttaatttt
    15541 aactggatta accttcctgt ttgttgaaga cgttcagcct gtgttttttt ggccttgttg
    15601 aacagactgt tcaatattcg ttcatgcaac tcaataattt catctgtcag cgtagctctg
    15661 gcatcttcca ggacacacac cagaattgca tgacgtcggg ctgctgaaaa tcgagtcagg
    15721 tccctactgc tcatctttcg cccttctcgc gcaagtttca gtaacctgtt ttgatgaatg
    15781 gtacgatcaa taccttccgg caatgccagt gattcaatac tgttcagccg atcaatgtgt
    15841 tgcagtacat tcttaccgtt gattttacct ggcggttgca gaagccaggt cagccgggaa
    15901 ggttgattgt ctgatgattc aagcaaacga tccaaagctt ccctgtgagc agaggttaag
    15961 ggcgcattta gcgtttgaaa gactatttta tcgccgccag ccatagcttc cgcacaggta
    16021 cgttccacaa catcaatcgc aggaaacata accttgtttt gccgaaggta ggccagcatt
    16081 tcttccgcca ggagaatgcc tttgtcagta cgagtcgcca ttggcaacag atgtgtaata
    16141 caactctgct gtattttgtt ggtgaatgct tttacaccaa gataacggta caactcggtt
    16201 aaatgctcgc gtcgagtagt ctccctgccg ttgatataag aggaccatag cccatcggca
    16261 agttttaacc tgttggctat gggttttaac aatgcatcag aggggggcgt tttcttatca
    16321 ggagcaaaac cgacattttt cagatagcaa agaagaactg caaatccaaa acgactggct
    16381 ggtttgcgat gagcattaat aagagccagg tcatgttcgc tgaaatatgc cattcgggtc
    16441 agggtcaatt catcatccgg caatgccagt agagattctc tttccgactg cgaaagaatc
    16501 tgcctccgtg ccatattcat acctcacata gtacttttaa cgaaacaggt tatcgcataa
    16561 gggtacactc tgatgcgaga tataaaatgt gacctcatac aattaatgac ataacctata
    16621 tgcgatagaa tatatgcaac aacctaaatg cgataatgcg gtgaaaaaat gttcatcaga
    16681 gcttatttaa gggcgtcaac agaagatcag tttgcagatc gggcaaaaga gatgctggaa
    16741 cagttcgtcc agggacgggg gcataaaatt gccagttatt atcgggagaa cataagcggt
    16801 acaaaacttg accgtccgga actggggcga ttactgatgg acagtcaccg taatgatatt
    16861 ttactggttg agcagataga ccgcctgacc cgtcttagca acagcgactg gataacgctg
    16921 aaaaagcaaa ttgaacaaca tgaattgcga attgtcagtc tggatgttcc aacatcatgg
    16981 caagcccttt cagataaagc tccttcacaa tcagatccga ttacccgcgc agtgataact
    17041 gcaataaata atatgcttat agacctgatg gccgcgatgt cgcataagga ctggctgagt
    17101 cgtcgccaac gccaaaaaca gggaattgaa cgagctcata cgttgggtaa ataccgggga
    17161 aaacaagccg atcaggaacg gcatcagaaa gtgttgtact acagacaagt aaaaaaactc
    17221 agcattagag aaacagcaga tgctacgggc tacagcactt cgcaggtttg ccgtattcag
    17281 gcactttatc gagaaccaac gactgattaa aaagtagcct taacgtacaa taattttcga
    17341 tatccaaact gacccctaac actttgttta gagtggacaa aactacgaga ggaaaacaat
    17401 cgtctttatg aaattaataa ccaggcaaac agaggatggc gtggtttcat cctaagattg
    17461 ataggggtta acctgccaga taaaaggact gagtttacac aacgtctctt attgacgcgc
    17521 aaaatttcgg gttcggtcat gaaaaagtaa catattctat gctggaacag accaattttg
    17581 cattcgccgt ccatgagttt gaggggaaag tagtcagtgt tttacgctca gtcagggaaa
    17641 gtgtgcaatg ataaatgaca cgaaaggaat cgtagtacat gggcaccgcg attcaatatt
    17701 caaggaaaac acaatacagc aatgtagttg ttctgttttt attattctgg cgatttcaat
    17761 aaaggcactt gctcgcgctc tgtcattttc tgaaactctt catgctgcat ttcgattttc
    17821 cttctaagat cataactcgc atacctgttc tttttatgta tatccatatc tatttttgtg
    17881 acgagttcaa accacaatgt ttgcactccc catgcagcgt tatgaaacat ttccgcaacg
    17941 gctttagagt ctgtattaac tgcttcagtc atgtagtcgc taatacgttc tgcggtaatt
    18001 ttcgttattt catcgtaatt cattttatct cctacaagat tatttaatgg aatgcaaaat
    18061 atctggcctg ataataatgt tccagaatta atacctgcag gctatgttaa gttgtcccca
    18121 cacacaatct acctgttttc tcgagtgtgc cacaggcgca atacataaat agtctggcca
    18181 ttaagttcgt aacggatttc gtactcgcca gcaaaaatcc gtctgacctc cctgggttca
    18241 aactgaaaca actgttctcc catacgtgga tgagttaaca gaatgaccgg tgcctgtgtc
    18301 agggactgta ccgttctggc ggcagcaggt ttactggcca gcaccagaaa atcatataac
    18361 cgcgccaaat cagaaagcgc cttactggtc cacttcagtt ccatcagcgt ggtaccggta
    18421 acggattgtc agtactgagg ctgtccgccc aggcctgtac agcctgatgg tcgataacct
    18481 gtccggatgt cacatcgtcc agggcttcca gcgtcaggcg attacgttcc tcctcctggg
    18541 caagccatgc agaaagcgcc tgtttgataa cccagccccg ggagcgttcc agcctggcgg
    18601 ccatctggtc gactttatca gccatgggta atggcacatg cgcggtaatt acttttgttg
    18661 cctgtgcggt attgtttttc atttgtatat ccgattagtt gttaatcata tttaatcata
    18721 gtgattactt tcagacgaaa caaggcaaat aatgcgcgtc agcgcatgta aggcggcata
    18781 cggtggttac atgtagcaca ggtgtgaaac cgccgaagcc cggcgtaagc cggtactgat
    18841 tgatagattt caccttaccc atccccagcc ctgctaagcc atacccgttt tcagccatga
    18901 cagagcttct gtgcgcggtc ggagtggtcc cgacgagggt ttacccgaag tcggggcgta
    18961 tctccgcgtt agcgggccgt cagggccgct tacgagcgtg tactgaggac ttccagccag
    19021 aagactgaca gcgatgagga ttcttagtta caacattcat aattaaaagc gatgttgttc
    19081 ccgcccttcg ggcgggggcg gcctcgcttt tcagttatga ggggtaggca cggtgctgcg
    19141 tttgtgtggt gtttttattc ctgatgtctt tttgttgtgg tttcagaacg gtgaggttcg
    19201 gggcccgcgc gtcagcgtgg gaggtgcata tttgtctgtc cacaggacag gcactgaata
    19261 ggtctttttt ttaaatggaa gtagttaagt agtttaaagg aaatacagta ctggggttta
    19321 aaagaaacat tgcaccctgt aacgcctgcg gctggcgctt tatagcatta acggttgtaa
    19381 ccttacggag aagtcccttg cagttaaatg tggataaaca gaattccccg tctgtgagac
    19441 gtgttttgcg tgaaaaacag ggggagcaag gcgagccgga aggcagatga tgaggctgtt
    19501 ttttgtgtgc tgtgctgctt ttttgtgcgt cgccgggctt cagacgtgcg gaagcctccg
    19561 gcgcaggccg gattattcag atgaggtcaa actaagggtg aggcagtggc cagccgtgtg
    19621 tagttgttgt tccgcgacat tatcatgcgt tccttcacgc gacggtcgat ttcgcgcttc
    19681 acggcctcca ggcttccccg gaaacgccct tccgcgattt cacgtgtcag ctgccgtttc
    19741 accagggtga caatgtcctg acgggtcctg cctgcatcgc ggcgcgcacg ggcacgtttc
    19801 atgccgtggg acttaagctc tgcctgataa ctgcggaaac gctcgcgcac aaaacgccag
    19861 gccctcgcta tcagttcatc catacccagc gcatccatcc cctgcttttt gcgcagcctg
    19921 ttttcccatt ccacgcggct gcggcgtgcg gaagccactg cctcttctga gatatcgagt
    19981 gagtcaaaca gtgccggcgt gaacgtgatg tcggtcggga tgttacagcc aattgtcggg
    20041 tcgtattccg tctggtaggt tatcagcccc agctcagaca ggaacttcag ggcacgggtg
    20101 gcacgggtga tggagagttt cccggcctca gactccgtcg ccagtccgca ctcaatggcc
    20161 agcgtggtga tggagcactg cacgcggttg gccagcgggt catagtggaa acacagcccc
    20221 tgcagcagcg catcaatcgc ccggcggcgc agcaccggcg gcatacgccg gcgcaggccg
    20281 cgcgaacggg catacgccac atgcgcggca aaatcaaaac gggaggtgaa gcccacggcc
    20341 ttttccatca gtttttcgca gaacttcagc gttccggcat cttcacgggg tgtgaacacc
    20401 ggattcgggt tctttacctg acggtatgtg ctttcagtaa atgaggtctg gttcagggcc
    20461 gccacaagct gtcgcttatg gcgggaacag attgccgtgc gttaatgcac tgcaaagggt
    20521 tgaatctctg acacggtttc atatatactt atcccgtatt ctgtgaggcc cccattatct
    20581 ttctctgatt ccgccaagtt tcgaggaaag atgatggggg ttttccttta tggtcttctg
    20641 aaactttttt cagccttccg gccccggcaa actgccgtcg tttctgccat acatcctgaa
    20701 tgccttatgg cgcattctgc cgttttctgg ctttccggac cgcgaggatc ccaaaagcca
    20761 ctgcataacg atccgcaata tacccctttt ccaccgcttt gaaaagccga cacgatttct
    20821 cagagttaca gttgccctct ttaaccccct aaaaagcgtc gtttttttga actctgtcaa
    20881 aaaaatgaac aatttcacaa acgatctttt atgctgcttt tttcttcttt tccagtagaa
    20941 gagatcgccg aattttttta aacgatctaa tatcccgcac acaacatcaa atttagaata
    21001 atattctgtc ttatcaaata taacgatcta ttcaactgct tatcctcgct cttgccacag
    21061 atacatcctt atgtaatata ttttccagcc attaatcctg tttgcaggag ttgtcggttt
    21121 tcgcccgcct cgaaaccgtc cacaaggctt acaagagtgc gtaaatcatg tgcctgtcag
    21181 aactgcaggc caggctgaac gtgggtcagc cgctgtttcc gacacatgct ttatgtattc
    21241 gccgttcaca acaacatcga agcccccctg cggggatcga ttttcgcgga gcgttccgga
    21301 ttttgaatat cagtgaatta catcaacgca actggtatct ggcacagtac atccctgccg
    21361 gtaaaaaccg cgaacatctc ttttcctggc tttccgaaca acacgtcctt ccctggacac
    21421 ccctgatact gaaaaaggtc cgccgcacgg ataaagtctg tggttaccgt cgacatatac
    21481 acgccgtgtt tcccggttac tttttcctga aagcagaccc cgaaagccac agcttcactc
    21541 accttcgccg tcacagtgcc tttctggact tcgtgaaaat ggccggtgaa atcaaaaccg
    21601 tcagggagga tatcgttcag tctttgatga aagtgtaccc ggatcctgcg ctcaatccgg
    21661 cagccaggga ggagctggat gctgcctcca ccctctggct gacaaaagcg cgttaccagt
    21721 acctcctccg gcttgatgcc cagcctctgc cggagtcgag gatcgccctg ctcctgcacc
    21781 tggtatctga tgacggagcg ttgacatgaa aattgctgtc ttaatttgac gacttttccc
    21841 ggcctttatg gccgggattt ttttcccttc tgtttccaca atgcccggca ccggtgacac
    21901 ctgagggtgt acggagagcg gctgtcgggc attttttcag gacgccttta tgaccattct
    21961 atcccaggaa aaccctgtca ttctgttact ggagaatatg ctcgaagcac tctccaccgc
    22021 cccggacaac ataaacaacg aacggcgtcg ccggcgttac ctgctgaact ggcttgacac
    22081 tgcccgtcag atgcgcgagt tcagaggaat ggctgaagag ttcactaccc tgcgaaaact
    22141 gctggccacc aattcagaca taccggtagc aaacaccctg aaatcactgc tggaaagtgg
    22201 caataacgca gcattatacg acctgtaccg cttccgctcg gctcttgcca cgtgcaggag
    22261 tctgggatgg cagattggca cgtgcgcatg gaatgaccag ttactgagtg agacactttc
    22321 ccgcgcacag accgggaaac gtcatatcct gcagctgaac agcaccgggg acaccttttc
    22381 caccacgggc agaatgatca gaccggtggc attttcatta atccatccgg ccaccatcaa
    22441 cacgtctgag gttgaagaga tattccggga tgaaggtttt atgctcgcac acgggcgcga
    22501 atgcagtctg aacggcagcg gcagaaatat gctgtcacgc attcttcatg tgggccattc
    22561 aggtttagcg gaagaggaat ggggcatgga caacagtctg cttttacact gaaataccgg
    22621 gacagccaca acggcagcca ctctccgctg actttttttc gcaaatcgcg gtttgctgat
    22681 gtggatgtcc cattttttat tatccgtcct ctccggttgt cataaagcgc attccggcgt
    22741 gaggcttctg ttgttatcat cccctcctgc agacatcccc ttgtcttctg atgactgacg
    22801 atatgcttct tctgacaatt aaagaagcgg aagcttcacg ccataatgtt gtttgactga
    22861 cctgccacag gaggaaaaat gacactgacg ttacttaccg cgtataacgc accctatctg
    22921 gcagccctga cttttgttct gcttactggt atcgctgaac ttatcgccct gctctgcgga
    22981 cactccctct cttcagctat ggatacacct gatttaccgg agggtctgac aggagaggcg
    23041 ctggactggc tgaacatcgg acggatcccc ctgctgatcg tcctctgtat gctggcaggt
    23101 ttttttggta tatccggcat tctcctgcag ggtcttatta ttcatctgct gcaggctcct
    23161 gctccgaaca ttctccttgc accactttgc ctgctgctga catgtcctct cgtacaccgc
    23221 accggcagac tgattgcccc ctggttaccc cgtgatgaaa caacggcgct caccgaggag
    23281 gcattcatcg gctgtatggc caccatcact ggttatcacg cagtggcggg ttccccctgt
    23341 gaaggtcgct tcacagaccg cttcgggcag gttcattacc tgttgctgga gccggaggcc
    23401 gggaaagaat tcagacgagg agacaaagtg attattgtct gccgtctttc cggttcccgc
    23461 tggctgggcg aactgaatcc ctggccggat gttctttaat cccgacatat acagaggttc
    23521 tcactatgtt taatcttccg ggatggttgt ttaccgttat agcgctggtc ctcaccctgt
    23581 tcgttatcgg gctgatattt gcccgcctgt accgccgggc atcggcagag caggcctttg
    23641 tccgcacagg tctgggaggc caaaaagtgg taatgagtgg cggtgcaatt gttatgccca
    23701 tttttcacga aacaattccc gtcaacatga acaccctgaa gctggaagtc agtcgtgcag
    23761 cggctgaaag tctcataacc cgcgaccgca tgcgtgttga tgttgccgtt gccttttttc
    23821 ttcgggtaaa accatcagcc gaaggaatat ccactgcagc ccagacgctg ggacaacgca
    23881 cgctcacacc tgaagatctg cgtagtctgg tcgaggataa atttgttgat gccctacggg
    23941 ccacggcggc cagaatgagc atgcaggatt tacaggatgc ccgcgagaac tttgttcagg
    24001 gtgtgcagaa caccgttgcg gaagacttat ccaaaaacgg tctggagctt gaaagtgtgt
    24061 cgctgacaag tttcaaccag actgcacggg tacattttaa tcctgacaat gcctttgatg
    24121 ctgaaggcct gacccttctg acccaggaaa cagaacgtcg tcgccgcgaa cgcaacgaag
    24181 ttgagcagga tgttgaagtg gccatcaggg aaaaaaaccg ggatgccctc tcccgacgtc
    24241 tggagattga acagcaggaa gccttcatga ctctggagca acaacaacgg gtaaaaaccc
    24301 gtacggcaga gcagagtgcc agtattgctg ctattgaagc tgaacgacgt cgtgaagcag
    24361 aatccgcgcg aattctggct gaacgtaaga tagaggaagc cgaaatcgaa cgacagcaga
    24421 tagtgcgtac ccgccaggtc gaagccgaac gggaagtggc catacgggaa attgagcaac
    24481 agcaggcaac cgaaatcgcc agccaggcca gagccattgc cgtggcggca aaatcggaag
    24541 agcagtcaca ggcagaagcc cgggcaagta aagcactggc tgaggccgta caggcccagc
    24601 aggatgtgga aaccacccgc cagactgcgg aggccgaccg taccaaacag gttgccctga
    24661 ttgctgctgc tcaggaagct gaaacgaaag cggttgaact gacggttcgc gcacaggctg
    24721 aaaaagaagc agccgaactc caggcagcag caattattga actggcggag gccacccgtc
    24781 agaaaggtct ggcagaagca gaagcacagc gcgcactgaa cgatgccatt aacgtgcttt
    24841 cggatgagca gaccagcctc cgcttccgtc ttgccctgct ggatgcatta cctgccgtca
    24901 tagagaagtc cacagaacca atgaaatcca ttgacggtat taaaatcatt caggtggatg
    24961 gtctgaatgg cggtgttacc cgcggtaaaa cagcaacaac cgacataagt ggcggaaatc
    25021 tcgctgaaca ggcagtaacc gccgctctgg cttaccgtac acaggctccg ctcatcgact
    25081 ccctgctgaa agaggtgggt ttatccggtg aaggacttac agccctgaca cgtccactgg
    25141 tcatgcctgc cccggaaaag gttccggatg tcagcgaacc ggtaaccgct ccggataact
    25201 cagaaccaca acaggtttaa tccccgtgcc ggaatttttc cggcatcttt ttcacaaaga
    25261 atgccctcac gggatattct ggtttaaaag aggattgaaa atgtccgcag aaaaattcag
    25321 agttgtcata tacaccaata catgtgaaaa gaaagtcagt tatattcctg caacagaaga
    25381 tataaacatc gctataatgc catttcagac ggcagacaat ggcttaatta ttgtgtgtca
    25441 gaactcccgt ataatacgac gcaaagagcg tattcccgga agcagctgtg aatggaaaga
    25501 tgtgacatta gcaccaaaat ccagaaaccg cgttcagtta tagcctgaca gcctgctttt
    25561 catcccccat aaaagggggt aaaaattaaa tatttcacaa gcgcgatatt tttcaggaat
    25621 gcagattatt ttctgttatg accaattgag aaacaggcat gagaacaatc acaacacgtg
    25681 aacaattatt aattaatggg agagtccacg aaagaatcgc cacacatata gttacaggag
    25741 ctcatgggta tgaaacgctt tgcacaaatg gctataacgt cagatacaac aaggcgcagc
    25801 aactggttga aagctgcgaa aaaatagctg aaggtaaact acctgttacc tgtcctgtct
    25861 gtttcaccat atggcaggat gtacacagat tcacccacgt tgactttgac acacaatcgg
    25921 ggaaaagtga ttttattgat acctgccatt cggagatcat aaccggacat gtttcaataa
    25981 caggcaagaa ataaaataat gttccagttc tgaaaaacaa tatcacttgt tttctggaaa
    26041 ctatcatttg cagcaggacg tttttctttt tatatttccg gaaccggaat attttagttt
    26101 tcataatcac cccgaacatg aatgttcatc atcaatatcc tgatgaaatt atatttatct
    26161 gaagacctgt taccagccac ctgtcttata ccgtaacccg atacataccg gatttattct
    26221 atgaaaaaga aatcacttac actccttctg ccgtcattac tcagcggctg tacggcattt
    26281 aatgccacgc ctgttcctga caccacgcct ccggctctgg tgttcgtcga cggacaaatt
    26341 cgcgaaagcg ctgccattat cgcccatacg caaaaacgtc tgcttccgcc accggtgacc
    26401 cgccctgtga cgccgccccg cccgacaaca acagcgacgc ccacaaccgc agtcagcagc
    26461 cagagtaccg cactgcctcc ccgtccgctc cttcaggcca cacagctgcc cgcactggtt
    26521 atgaccggta aacccggtca gtcatccgtc gtttatttac gtgaggcaca tcagcgctcc
    26581 gtcagtgact ggatacgcca gttgctacca cccggctggc agttccggca acagaacgct
    26641 gccataccgg cactgaacaa acgcctcccc gtctggtccg ttaatgacca gtggcaccgg
    26701 gcactgaatg cgctgctggc cggccagcag ctttacggac atatggactg gaacaggaaa
    26761 atactgactg tcacgacctc tgcaacaccg ccggtcgacg tcacggcacc gcagggttca
    26821 cagaaagctg ctgatacacc gcgtaatccg ttcaggggca gcgccgcaac gccggcaggc
    26881 ccgacaccag tgacacctgc cgtcagcgtt tcccgggctc agaccaccac cgggacatcc
    26941 ggaactccag agagcagaac aaccacgcca gccccggtgg ccacgaaacc ggtagtgcca
    27001 ccacagcaga aggtcatacc gccacggcag tggaaaatgg aaaaggggac caccctgaaa
    27061 gacggactgg cccgctggac atcatccgaa aaatgcccgc acggcacatg gacactgcgc
    27121 tgggaaacac ctgtcaacta cagcatcgat gcccctctgg ttttcaccgg cagtttccgc
    27181 caggcgctgg agcagctctt tgcactctat accccggctg aaaaaccgct gtatgcccgg
    27241 accagcactg cacaatgcct gctccgtgtg acagaccgcc cgcaggaata acctatggga
    27301 tgggttacgg gtttcattat ggtcattatg gccattggta atttctggtt ccacagtcac
    27361 ctcagccgta ctgtgcacca tcagcagacc gctgaaatca cacagcaggc tgcagacttt
    27421 attcgttata tgaatgccat caacgattac ctgtatcagc accctgaacg acgtgctgcc
    27481 ggtggtcagc tcacgtcagc acagctcggt ctgcccgcca cgaaaaacgt cagccacctc
    27541 atcagtcagc agcgcgtttt tgtctgggca aaagaaaaac ccggccttat gggtgccctc
    27601 ctggaacaaa gtggtgattc ggccctgctt gccagagtgg aaaacggtcg cctcctggac
    27661 acccgtgggc ggcgtatttc catcacgctc cctgctgtga tccccgatca ggtcatcatc
    27721 tggatgaatt aggccggact ctgaataact cacggattta tctatgaaca aaaaacttct
    27781 tgctattatg attgcagcca ctctgctgca gggctgtacc agtttccgac aggcagagaa
    27841 caccagaaaa gacgccgttg ctgaacaaca acgggcccgg gaaatgatcg ctgagctgcg
    27901 aaaccggaca ccggttgtaa aggaatcccc acgtcagtgg atcaatcccc gtccggtcac
    27961 ggataaaaac cccggtaatg cggtgcccgg ctgtccgatt atcattaaca cccggcagag
    28021 catcaccctt cagcaggtgg cccagcggat ctcgcaaaac tgtcatattc ctgtaaggat
    28081 cacccctgat gtacaggcgt atctgagcgt gaccacctcc ggcagtacgc agcaaatcca
    28141 gggcacagta ccaccgccgg ataccggcgg tatggttcca ctggccgcca taggcagttc
    28201 tgacacgaca cttccccgcc tgcagggaaa tacggcgtca cttcctgaac tgaaggcttc
    28261 aggactgaca tcactgctga atgcggtcgc ttcccgtctc gggatttcct ggcgttatga
    28321 tggttcacat atcacgttct attatcttga tacccggacc ttcccggtga cctatatgga
    28381 cagccaggtt gcctataatg ccaccgtggt cagcggtacc atgtcatcaa atggctcatc
    28441 aggtggaaca gcatcggatg ccatttccgg tgatgccagt aatacccaga ccaccaccgt
    28501 ggatatgaaa tctgcgctgt atcaggatgt gaaaaatgct gtctcatcaa tgctgacgcc
    28561 gggtattggt cgcatgttca tgtcgacggg ctttatcacc gtcacggaca ccccacaggt
    28621 actggaaacg gtccggacgt tcattgaaaa acgcaatgaa gaaatgaagc gccaggtggt
    28681 gctgaatgtg gaaattctga gtatccggaa aacccgtaat gagcaggcag gtatcgactg
    28741 gaacgcagtc ttcagcgaca gaacgctggg actgagcctc ggcagcacat tcaccagtgc
    28801 agccagcgat accgtcactg gtggtgtttc cattgtgaac gggaaactca ccggttcaaa
    28861 agccttcctg aaagcactct cttcccaggg agatgtcagt gtggtaaccc ggaattccgc
    28921 cgtgaccaaa aacctgaccc cggtaccgat gcagattgcc aatcagcaga gctacattga
    28981 gagtgtgacg acggatacca ccgccaatgt cggctcatcc acctccctga atgcggccac
    29041 catcaccacc ggttttaata tgacccttct gccgttcatt ctcccggact cacagacact
    29101 gcagctgctc tattccatga gtctctccga caaaccggtt attgagaatt acgagagtgg
    29161 tggaagcaaa gcccagctgc cgaatgtgga tctgaaaacc atcaaccaga cagtggacct
    29221 gaaatccggg cagacagtca ttatcagtgg cttccagcag tcagggcgtc gcagtggaaa
    29281 acagggtgtc ggcacaccgg gattcttcgg tcttggcggt gggatcaaca gtgaaaacga
    29341 cgacaccatt ctcgtggtgc tgatcacgcc caacatcatc tgatttgcag aaggaggact
    29401 atcgtgacag atatttcacc gacagagcga caggtgtttc cgctgacggc aggacggaat
    29461 gtatttcttg ccggaatgga ctggaaaaca ctgcccgcaa gccataaaaa cccccggacg
    29521 tttgcccgca gtctgggggc agtacgctat atttcctgtg agtatctcag tacggaagat
    29581 accgatcgtc acatcatggt tgcagccgtc agccagaata cactccccaa aggcagccgc
    29641 aggtatttct ctctcgccat gcttatcctg cctctgctcg agccaggagg atacgctatc
    29701 gttgaactgt cacaggcaaa cgatacagaa ctttatggtt ttgtttctgc tgttgacggc
    29761 atcctggtca gtgacctggt gggaacccgg gaggaaatca gggaggcccg ggaaatattc
    29821 ctcaccatta acagcgcgcc tgaacacggc tggacatgtt atgaaccgcc ctcgtttaac
    29881 gggcctgacg gcagaggtcc cctgccactg gaaaccctga caggtgcagg gaaatatccg
    29941 gcagaagcac gccttcaccc cgtatcccgt gggccacagc ttattccggt attactcata
    30001 ctcacccttc tgggtactgc atggtatggc tggcagtact atgaacgcct gaaggcagaa
    30061 aaagcagcac ttgctgaagc ggcacaaaaa gccgcagagg aacggatcac accaccgtgg
    30121 ctgagtatgc cggaaaccgg agattttatc cggggatgca gtgacgtatg gtcacgcctg
    30181 ccggtgtcgg tcgccggctg gcgatattca ctcgccgagt gtgctgccac cgggaacagc
    30241 ggcaatctgc gtgcatcata tacaaacatc gccgggacga cagtgaccgc attcagccag
    30301 cgggtcaggg aaattttcag catccggccg tttatggtta tgccggacgg gaatagcggt
    30361 ggttttgccc tgccggtcac cttcagtatg cctgaaactc ccgttgccgt tgaggcatta
    30421 ccggaaaaca ccctcctgca ggaacggctg accacactcg cccggtcgat gcagctgaaa
    30481 atggactggc aggaagtcag caacagcttc accgatgaag acggaaatac catccagccc
    30541 ccctggaagg agtatgacct gcagatactg acgacacttc cggcgcacca ggtggcagag
    30601 catttcagtg aaccctccgt ccgttttatc tccgtaacaa ggcaactgga agagggtcgt
    30661 ttccgttatc aattcacagg taaatattat gtccagtaaa acaaaagctc ttctgctttc
    30721 cgtcctgatc ctgccgacaa cggtcgcggc cagcaccatc agcaacatgc aacagaataa
    30781 ccggcaactc caggtggaac agattcagga agaaactgca ctgtatgaag ccatgctggc
    30841 aagagaaaag gtgcgacatg aactcagaac cctggagcag ggagaaccag ctgagcctgt
    30901 gcattcacag gaaagcagtt cagccgtcac gacagatacc cctgccccca ttaccggtat
    30961 tccccggctg acagagatat ccggagttca cccccagata accgccagga tacaactgcc
    31021 gggcggacaa actgctgatg taaaagaagg gcaacacatt cccggcacca cattccgcgt
    31081 ggaaaagatc accactgaca gtgttgtcct tgaacaaaat ggtatcagaa caacactaca
    31141 accctgactc agtgccgcca tttacagccg gctgtcggaa tagattattc ccccggccct
    31201 taatcacaaa aaaaatcgtt tccgggtacc gctgcccgta aggagatttt atgctggcac
    31261 ctgcagacca gccacctgat gacattctgg attacgtggt tgttaataca gataaaacag
    31321 gacaaattca tgtcagagtg gtggaaggaa aaaaacacct tcgtgccgtc caggattatc
    31381 tgacccgcct gtgctcccgc tatccgggta aagtatcccc gtttgagttc accacacttg
    31441 atgtgatcgc ccgactcagg caggagacgg tcatttccgg ggtggaaaac ggaattaacc
    31501 ccgtacagca aaaaatgctc gggtatatca gccatgcggc caccctcagc gcctcagatc
    31561 ttcacatcac gccggggcgt gacagtacgg atttcacgta tctggaggcc cgtgtccacg
    31621 gagagctgga gctgctcgat atcgtcagaa aagatgaggg gctggaatta ctgggggcca
    31681 cctattccgg aatgactgac gtcataaagg gaacacagtt tgatcccggt gtcccccagg
    31741 atgcccgtct ggctgaaaga ttcctgaaac tggccggact gtttggtgcg cgctactccc
    31801 actatccgtg tgtgggcgga ctctatgccg tactgcgtct gatcaaggat gacagtcagc
    31861 atataccgac attcagtatg ctggggtatc accctgagca ggagcgtgct gtccgaagga
    31921 tgctgcagcg accggagggc atcgtgatcc tctctggccc caccggttcc ggaaaatcca
    31981 ccacgctgag gacggcttct gccgcctatc tggaacagta tggcttcaac gataccggag
    32041 ggatcctgct cccccggcga cgtctgttca caatagaatc accaccagag gggcgtatcc
    32101 ccggtgcaat tcagacggcc gttatggata ccgctcaggg ctgggtcgat tctgttaagt
    32161 ccgccctgcg ccttgatcct gacgctatcc tgaacgggga gatacgtgac catgcctccg
    32221 ccatcacggc gataaaagcc gccatgaccg gtcacctgat gctgaccacc cttcatgcta
    32281 acgacccgat aaatattctt gagcgtctgg aaatggaggg cgttcaggca aggatgattg
    32341 cagatccgca gttatttatc ggcctgctca gccagcgtct ggttcaggtg atatgtcccc
    32401 actgccgcct cccctggcat gaggtggaga gctcccggac ggatgaagaa cgacgcctgg
    32461 tggaaaactt ctgccagccc gatgccgtgt atcttcgtaa ccataacggc tgcccacact
    32521 gctggcgtgg agtgaacggc cggaccgtca ttgcagaagt gatttccccg gatgcgaagt
    32581 ttttccagat ttaccgggaa aaaggccgga ttgaggcaaa aacctactgg caccgggagc
    32641 tcggcggtat gacacgaaat cagcacctgc tgggcaaaat caacagtggt caggtcgatc
    32701 cgctggctgc gcattacata tcccctgtag acgaggacag ctatacgctg ctgcactgat
    32761 atgaaaattc tggaaagact caacaatacg gaactggaac tgtccggcct taaccgctgg
    32821 ctgggcaaaa agacgttcag gcggactacg ttttacgagt cccttgcagg catgatccgg
    32881 gacggcacac cggttatgcg ggcactggag ttcatctgcg atgtggaaac ggattttggt
    32941 aaaaagaaag ggcagtccgg cctgtatttt ctggcgacag actgtatcgc cagcatacgc
    33001 agcagtggtc agctctctcc ggccctgaag gactgggtac ccaaagatga aattgccctg
    33061 atccgtaacg gtgaagaacg aggtgatatc gctgaagcca tgtttcaggt cgtcaaaacg
    33121 gcaaaaggac gacaggagat gattagcagt ctggtgagtg tctgcctcta tccgctcatc
    33181 ctgctgacac tctgtgtggt gaatatgtat aacgttcata cccgggttat tccgatgatc
    33241 agcgcctttg caccggaaga acgctggtca gtacagatgg tcattctggc caaatcctcc
    33301 ggactggtta tcggttatgg tttgtggctg ctggcaggac tgctgctgct ggcaattctc
    33361 atcaggatgt cattcagtct gtataccggt ccccgacgac ggtatcttga tcgcatcccg
    33421 ccatggtccc tgtatcagac tttccatggc gtgaattacc tttttaatat ttcatccatg
    33481 ctgcagataa atattccttt atctcatgcc cttaccagaa tagaaaaaca tgcagaacat
    33541 aaccgatggt taaaatcacg aataagtgct atcagaaaaa atgtcttatc cggtcagtca
    33601 ctggcactgg ccatgaaaaa ctgtggttat gactttccgt caaaatcctg tgtgaataat
    33661 cttcttttaa attcagaaag ggaggacagt gttgccagta tggcgcttta tgcagaccgc
    33721 tggctggaag aagcaaaaaa gaatgtcaga aaaagcggca ttattattac tgctatatca
    33781 ggtctgctgg tatttctctt catcatcaat atggtcagtg ccatttacgg catcagtgac
    33841 atgctgaagc agtaaacaca gcatccttta tattcccctc gatccaaatt ctgttttacc
    33901 aatctcagga gctaatatga gcgtcataac ctcttcgtat tcagaacata atattcgtca
    33961 ccttcagaac gatgataaag gcatgaccct gctggaagtt ctcggtgttc tggtcgtcgc
    34021 tgccattgtt attggtgccg tgatgggact gatgtccgat accctgtcct cttcagacaa
    34081 ccagaaggaa ctcaaaaacc tgcagacaat cgccaccaaa atgaaagccc agaaattcca
    34141 ggggcagtat acaggtactg actacgtcaa aatcctgacc gaaagtggtg gcctgcctgc
    34201 cgatatgatc gccggtggta ataaggcgaa aaatgcctgg ggtggtgcgg taaccatcaa
    34261 agtcagttca gataaatact cctacgttat tgaatccagc aatgtaccga agaaaaactg
    34321 tattgatctg gtcaccagcc tgcgcagttc atccatgttt acaaaaatca acggcaatgt
    34381 gacgaataaa gttgacccgt ctacagtatg taacgcagat aaaactacta tcaaactgga
    34441 aaccaacagt taatcattca tcagcgcccg cccgggcgct ttatttaagg tcactgtatg
    34501 aacaggttat ttatcctgac agtgattttt tcactgttat gctcacttcc ttccgcacag
    34561 gcatccccac aggacaaaca atatgatcgt tgttttatta ccgccggaaa acgttattcc
    34621 attgatccag gattactcaa aattattgcc cgcagggaaa gcagtctgaa tccccgggcc
    34681 attaatcata acaggaatga cagaggaaaa attatcagta ccgattacgg gataatgcag
    34741 attaactcca cacatattcc cgggctgaaa aaaatgggca ttatccggag tgagaaagat
    34801 ctgttgaata atgtctgcct gaatattcag accggagcat ggatcctcgc ccggcatttt
    34861 cagcgatgcg gcgtaaactg ggaatgcctc ggcagttata acgccggctt cagtaaaagt
    34921 aacactcacc gccggatgaa atacgcccgg cagatttaca gcgcttacat gcagggacgt
    34981 tagtccggga gaactgccat gctccagata atcccggcac tgctggtcgc cataaccgtg
    35041 gtaatacagt gtctggccgt tcagatgagt cgctatcgac tctcccggat gaatgcccgc
    35101 ccccctcacc atgggctggc gctgatgagc gggatgcttg cagctgccgc cattatcctg
    35161 cgtctttatc agaatgacag cgctgtcggg gtgctgttta tgacgataat gaccggatgt
    35221 atgacactga tgaccctgac ggatgctgca tcctgccgtc ttccccggcc cttcaccatc
    35281 atgtatctga taagcggcag cgcatttcgt ctctggcagg gagactggct gacaggcctg
    35341 tgcagtgcag ggttctggtt cctgctgttg cttctgttca ggcaatacac ctcttaccgc
    35401 aggaaaacag aaaccatagg cctgggcgat gtttttctga ttgccggtat tgccatctgg
    35461 tccccgccgg gagacatccc ctggattgtc gcaacggcag caggcggagc cttactttat
    35521 ctgtggtgta acagacaacg acgcattacc agagaacttc cctttgggcc gtttctctgc
    35581 gcgtcacagt acgcatttac tttttatccc ggtggccttt ggtaataccg gagggaacga
    35641 ctatgcaaaa agacaacgat aaaggttttg cacttcttga aattctggga ggactggttg
    35701 tcatcagcct gctcatgccg ctgttctggt cttacattga agactacctg aatgaaatgc
    35761 gcaaccagag cgccgcattt catgcagatg cctacaatac tgcagccagg acctacatcg
    35821 ccgataacaa tgcccgcctg catagcggaa cgcttcctgc caccttcacg gcggatgagt
    35881 taatccggaa aggatatctg aagggtctta accgctcgcc ctttgggcag agttatacca
    35941 ccggcatccg tcgtaacacg agtaccggtc gtctggaggc cctgacctgc tccaccggag
    36001 gggagaatat caaagacgat gccctgcgca gtatcgccag cctgctcccc ggtctgggcg
    36061 gatttatagg aaaaaatggc acagcaaccg gtgtttttgg tggatggaca gataaacccg
    36121 gcgactatgg actcagctgc aatggcgggc acatcgccat cgtaatgatg ggtgatgacc
    36181 tgcaggaaag cgatcgcctg tatcgctttc aggtaccggg acgaccggag ctgaaccaga
    36241 tgaacacggc catcaatatg ggagggaaca acctgaacaa tgcaggaaac atcaatggac
    36301 aaagtgcaac actgaaaggt gatgttacaa gcgaaaacgg ctggttaatc accaaaaatg
    36361 ataaaggctg gaagaacatc acctatggtg gcggttttac gatgacagac agccagtgga
    36421 tacgcgcagt cggtggtaaa ggaattataa ccacagggga aatcaaagga ggaaaagtca
    36481 gtggggggac agtccggtct gacggtcgcc tttccacagg tgagtattta cagctggata
    36541 aaacagccgt agcaaataca aagtgttcac cagatggact tgtaggccgc gacagcaaag
    36601 gggcaatact ttcgtgccaa tccggtgtgt gggttggctt tgaatcctac cctgtgggaa
    36661 gccctatccc gtggccatct gccactcctc cacaaggcta tctgttgatg aacggccagt
    36721 cgttttcctg ttcccggtat cctcagcttg cccgtgctta tcctagctgt aagttacccg
    36781 atctgcgcgg tgtttttatc cgtggctggg ataacgggaa gggacttgat ggtgatcgga
    36841 acagacagct cctgagttat caggccgatc aatcaggcgt ctatcacgaa cgtggcggat
    36901 ggctgaaagg gcatcatagt ggtatgccat actgggcaca ggggagtacc acagaaatgc
    36961 gtcccaaaaa cattgcattc aactacattg ttaaagccag ctagggactc acatctgata
    37021 ggcatacaca gagaaaacac cgttaccaca ggcgtaattt tgtgttggtc tggagatgat
    37081 ttgatatgac gctcccactg gcacggcaaa tgaaataaat ctccgggcat atcagctggt
    37141 cagcaaactc gatgccaggc gtaaacagac cagcaaaatt tccccttatt ttgttcccta
    37201 ccctgcaact gtcgggtgca gaaacgggct gtttgtcgtg acactgcacg atttcgacct
    37261 tgaaacccgt gcagaaaccc ggggactggc aatatctcat gccagcatgc tcagtcacct
    37321 cctgtccgaa tccttttcat tcgcccttaa aacagagttg caaacactct tgaaacacac
    37381 tcgatcataa cgatcgaaat aactcgcttc cgtttaaaaa acactctctt tagactcaat
    37441 ttatgaacaa aacacgctga cccgcatgga ggtgaaatga aaactgacag cacaggtatt
    37501 gccgcccgta tgatgctctc cctggacagg gagcgaattt gcgaatgcct gctcagtcat
    37561 cgccagctgc agtccacacc cctgcaggtt cgttatccgc agggagtcag ggatgccctt
    37621 ggtatcatga gcgaacagct ttctctttcc gtctctgacc tgacgcgtat cctggtcgaa
    37681 gacgcgttaa gtgaaatgtt tcttcctgca gataacatcg tccgccgtct tctcagcagg
    37741 atggagcaca tcatgcaggc acatgatatc agcgcaacaa cgatggctgc attactggcc
    37801 ccctggaata tccgccctgc cgtcttccgg gagccggaca gactcactga ctatctgacc
    37861 ggggagattc ttgcagcact ggctgactgg ttttatctca gtccggaatg gctgaacggc
    37921 agggtgcatt atccactgta tcgtccggga gactggccag ccacacagga gatattctgc
    37981 aggattattt cagcccgcga gaatatggac attattctct ggcatggttt tccgtttgcc
    38041 ggaacgcatt ccggagaata ttgcggcgtt ctgctcaggc agaaaaaaga aattaataac
    38101 accattattt atccggtact ttcgttatat ccggccagaa tggatataga aaaagaaggc
    38161 tggtttcaga tggcccgaaa aatatctccg gatatccctg ttcgcgccgt aacactcact
    38221 ccggcacagg cagaatatct gataacagga aaaatattac ccactgcgtt attcagggtt
    38281 ccgttatccc cctggtaata atactgagca ataaaccgga ataatacacc aactgttttt
    38341 aatggcacag ccgtgccctc tgttgttata cccaaaatac ctcaatacag gaaactgaaa
    38401 tgatgatgaa aaagcatatt acccgtactc tcatcgcgtc tgccgtttta ttttccttta
    38461 attcgactgc agcaacgtca tattttgaag cccgcaatga tgcgatggga ggaacggggg
    38521 ttgcctcctc tcactatggt gtggccccac tggcaaaccc ggcactgctg acaaaatccg
    38581 gtgcaaaaga tgacttcagt cttctgcttc cgtccgtggg tgcgcagctg tccgatccgg
    38641 gtaatatcgc cgacaacgcc gacaaaatca gcgatgactg gaaagccttc gaccgtgccg
    38701 tcgacagcca ctccggggta ccgcagacag cggcccgcct gaaagagaga ctgcaggact
    38761 tccggaacac ccatgccagc gcacaggccg gggtatctgc tgtggcggcc cttcccggtg
    38821 ataccctcgc tgctgcactg atgctgaaaa cgctcgggac tgtcagcgtc gacggtaaag
    38881 tcagcgatgc cgacctgaac tatctggaaa gcattgcaga cagtggcagc caggatgtgg
    38941 ataaaaacag actgacctca caggcttttg cccgtgccgc actgatcacc gatgtgggcg
    39001 ttgcgctggc cactgaactg gaaacggccg gacaaaaatg gtcactgggc ttcacaccaa
    39061 aattccagcg tgttgacctg tttaactaca atacgctgat caaaaactat gacagcagcg
    39121 cattcaaagg tgaccgttac cacaacaccc aaaacggtat taacgctgat atcggtgcca
    39181 gcatggacct ggatgacaac tggacgctgg gtctggtcgc acagaacctg atcccacgca
    39241 gcattgagac caaagaggta catggtgtca ctgaaacctt caggatccgt ccgcaggtaa
    39301 caaccggcgt atcatggcac aacgacatgt tcaccacggc actggatgtg gatctgaccc
    39361 cggccagcgg cttcacatca gacagcaaac gtcagtttgc cgccgtaggt gctgagttta
    39421 atgcctggaa atgggcacag ttacgtaccg gttaccgcca gaatctggcc agcaacaacg
    39481 gcagtgcatt cactgccggg attgggctct caccgtttga tgtgattcac attgatgtcg
    39541 ccggcctcgt gggcaccgac aacaactacg gtgccattgc tcagttacag ttcaccttct
    39601 gattatcacg ccccgggacg cccggggctt ctgacctgaa tctgctcgtt tccgctcttc
    39661 tggccactca ccgccagtcc tgttcattat tttccggata aaaccatgca aaaaaaacgt
    39721 atcacccttg cagggggagt tgccatgctc tgtctgagcg gctgtcatct tcccccggat
    39781 aataccgcac cggccaggaa gtacggtgca gcttctgaag tcgctgtcac acgacatgca
    39841 cagtatcagt tgtgggagca gggcacgtat tctgcagccc tgccttcccg ccagcccgga
    39901 ggcagcctgc aggctcacag cgggcgggtt tcctttgact gggaaggcga tgctctggag
    39961 ctcctgagcg agctggcccg tctgcgcgga caacagttca gttacagcgg cgtgcgtctt
    40021 cccctgcccg tctcactcca tgtacgcgat atgaccttcg ctgatgccct tcgtctcaca
    40081 gaggcccaga ctgcctggcg ggcagtcctc tcacagtcag ccgggcgttt acatctttcc
    40141 tatatgccaa cggagaaaaa atgaaaaaac aggtctgtct cctgctgtcc gggctcctgc
    40201 tggcaggctg tgccggacag caccatcgcc cccctgccgg tatggcaggc aatttaccac
    40261 cgccggatct ccgggcatgg ctgacgccca ctgacaacca ggatgacggc atttcagaca
    40321 cccgtcgcca gatgctcacg gacgccggac gaacgctggg attccgtggc ggaaaagcac
    40381 agcgctcatg ggaactgatt caggcactga atgcccggga aagtacactg aatgccctgt
    40441 atgacttccg tccccttatc agcagggaag gctggctacc ccctgttatt gatgaagcgc
    40501 aggatgttgc tcacattcag cctgaccaga taagaaccgc gtcccgtgtc tggaccatcc
    40561 tgcgccccga acgttttgtc agtaacccgc cgggctggcg tgactggctt ctgcgggggc
    40621 tttccactac cgccaccccg ggaactgagg gccgtgttgt accggaagac cgcgcacagc
    40681 gcagactctg ggaaaacgca ctccgccagg gctggcagga aggccgtgac aacgctgacc
    40741 tcaccctgga agccaaccag aaacgcctga cccgggatta ccggggaatg atgctttatg
    40801 ccctgctctg gcgtcagggc atgatcacac gcccggatgt gactgaacag cggcagaccg
    40861 tcaccggtaa cggcaggaaa ctcatcaccg gagaccacgt ccgccgtctg aagacacacg
    40921 cggaattcac cctgcagaaa tcacgctggc gtccggtcgt cagcacggaa ggagcccccg
    40981 atgaaattac ccgatgagtt cggcacattc cctttcgccc gtttcacggc cgaagagttt
    41041 cgctattttt ttgcctggtg tgcccgacac cgcgtcagtg atatcgatct gacaggcggc
    41101 tcaccggtat ctgtcagtcg ctttggacgg cgggtacgtt cgtccgcaca tcctctgccg
    41161 tcctccatca tgatggcgct gagtgatgaa ttgtttggac gggaagtcct tccccgggtg
    41221 ctgtccggtc atcccgtgga ccgtaccatt caggtcagcg gtgatgcctc cggtcgctac
    41281 ggtctgcaac gggggcagcg tgttcgcctg cgctgtcatc tgattcaggg aacatcagca
    41341 acagaagaaa aagtaatctc cgtcaccatg cgggtgatcc cgtccgatat tccggacatc
    41401 ctcaccatga atattgaacc ggacctgctg gatgccatgc tccggaaaac cggtctgggt
    41461 ttcatctgcg gagagaccgg ctccggtaaa tccacactgg cagcggcact gtaccgctac
    41521 attcagacgc atttccccga ccgcaaaacg gtgacctatg aagatccggt ggaatacatt
    41581 ctggggggag agaatgacct tctgccacca catcaggccg aaatcgggcg tgatgtggaa
    41641 agttttgcgg caggacttcg ctctgccgtt cgccgtaacc cggacatcat cggtatcggt
    41701 gaaatcaggg attatgaaac ggcagatgca gccgtccagg caggtaacac cggtcacttc
    41761 tgcatcggca ccatgcacac caaatcaccc ggtgaaacct ttgcccggct gctggggtta
    41821 tttccaccgg aaatcaggga ttcaatggca gccgccaccc tttccctggt gcagttcatt
    41881 ctggtgcagg tcctggtcag aacaaatgac ggtggcagac aggccgtccg ggaatacatc
    41941 gtcataactg acgaactgcg tgatacgctc tcccggcagt cccatgcgac atgggggcat
    42001 tacattgatg aaatcattcg caaggaaaaa cggcggatac gggatcaggt tctgaccatg
    42061 tatcagacgg gcagcattga ggcatcagaa gctgcgctct tcatcccggc cggagaattc
    42121 cccaaatgaa acgcgaaggc ttctggcggg atgtcactcc gccactgaat atttatggtg
    42181 tcccgttgcc gtttaccatg atttacctgc tgctgtttcc gtatccgtca aaaacaacat
    42241 tctgggtgtg cagcggcatt ctgctgtttt ttgtgctcct ggggtactca ggatggactg
    42301 tccggacgct ctgccggcgt ctgtacagcc tggtgcaggg taaacaggcg tcaggccgtc
    42361 cctggtggta ccggcatcgc acagaattac cccgtgaatg gcgcggaacg gaataaccct
    42421 cttttgttca tcatcccggc gtatcacaac gccctcatct ttaatccccg gagaacgaca
    42481 tgaagtccag atatcgaacc tggcttgctg tcccgcctga ggagacagaa gccgtaaaaa
    42541 atgcagttcc cccgttaaat ggccgcaaag ctgtcgcatg ggatccagaa aaaaagctct
    42601 ggtatgcccg ggcgggtacc gaactttcac tgctggagcg ctggttaccc cggccacagg
    42661 aactgtcgat ggatgccggc gatccggtca ctgaatttgc tcaggtcctg gaaaacgccg
    42721 ggctggttat tcagggattg ccacagatgg acggagccat acacagagtg gccacccggg
    42781 atgacaaaaa aggggccaaa agtggcgcat acagggccta tcttgatggt cgtcccgccg
    42841 gctggtaccg cgactaccgg agcgccgatg acagtcccac aaactgggta ttttccggcg
    42901 gagaacaaca cgatcctctg gccagactgc atctgcgggc attcgctcag caacagcgcg
    42961 atgataacgc tcgtaaactg cagcaacagt ataacaaaca ggccggctat gcccgctcct
    43021 atattaacgg gttgccacag gcaacagcac atgaatacct gacgcgcaag ggcatccggg
    43081 ccgcgcctgg cgtgcggctg aacaataaaa atgagctggt tattcccttc agcaacggcc
    43141 ggggagaaat acgttcgtac cagcgtattc cggtcaccgg cgggaaggat gcccgcattc
    43201 tgaaagactc cgagaaaacc ggcaactggt tcaccttcgg aaccccggaa aatggccgcc
    43261 ccctgttatt tgcggaaggt tacgctactg ccgcctccct gcacgaagca accggccttc
    43321 cggttctgat gacagttgat gccagcaata tgattgccgt ggcagaaaat gcccgccaaa
    43381 tctggactga cagccccttt gtcttctgtg ccgacaacga tcatcaacgg gaaattaaca
    43441 aaggggtttt tagtgcaaca aaggccgcag aagtcacgaa tggcgaagtc attattccgg
    43501 cctttacgga ggcggaaaaa gcacagggac tgaccgattt caatgacctg gacgccagcc
    43561 ggggacggga caactttcag aatgccatga atgcacagtt aaaacatatc ggtatcccca
    43621 cacccaacag tgacactgcc gaccatcgtg aggctgtcgt gatcgacaac ctgatattca
    43681 caccggtaaa agatgagaag ccacaaatgt cccctgaaaa cagacagagc acagccccgg
    43741 aaacggagct ggcgacacag gatacgcctt atgacaccca ctccgacgat acagaaaacc
    43801 aggcatatga agatgactat gccgcctatg catccctgca atcagtaact gcggagacgt
    43861 tatccccgga taaaccactg gagaatattg cgtctgccgc tgataccggc acagagaata
    43921 cctctgtgcc taccgctggc gaccaggaag acctcaatac agacgccaca ggtacagaca
    43981 ccgctcacaa cagcgacaca gcagaaacag gaacgcttcc tgaacctgcc gggatgcagt
    44041 ccgtgcagca ggcaaccact gaaccgaccg cagaagataa accacacgag aacgttgttt
    44101 ccgctgccga taccagagaa gaaaatgcct ctgtggctac cgctgatacc ggtgaagaaa
    44161 atgcctctgc caccaccgct gacaaccaga gcacgcaggc agaagaacat ccccggagtg
    44221 aagacacacc ggaaacctcc ctctccgacg cggccgggac acagcacacc accacaacgc
    44281 agtctgaaga acaagtgcag gtttccatgg ccagcgctga cgactggagc gcatttgcgg
    44341 aagatctcaa cgctgacact ccgggggccc ccactgttca caacaccgac aacgcggaga
    44401 caaacacagt ttctgaaccg gccgggatgc ggccagttca gcaggtgacc actgaactga
    44461 cggcagaaga taaagcacag gaaaagagcg ttgcgtctgc cgctgagacc ggcgcagagc
    44521 actcctctga catcaccgat gacaacagga acgcacccgc tgaggagctc aatgccgatg
    44581 ccacggttgc tgttcacaac accggcacag cagaaacagc ctcaccttct gaacctgcag
    44641 aaactcagcc agtccggcag gccaccgctg aaccgacggc tgaagataaa ctgcaggaag
    44701 agcgtcttgt gtccgccact gagaccagca cagcgaatga agcagcccga cctgaaacgc
    44761 catcgctttt cagccgtctg cgccgttttc tgaccggtgt gaaatcacgc acctcagaac
    44821 cttccgttat tcaggagaaa aagcccacgg aggctgaaac agcaacagag gaggtcgcac
    44881 caccatcacc accggaaacc agcccggaag acacatcgtc agcggaaccg gacacgatta
    44941 tctttgcacc ccgccgtccc gacagtccgc agcggcacaa cctggacgag atccttaaaa
    45001 atcttacaca caaagagttt cccgacagga ctgcgctcta cagcgttgac ggtgaacccg
    45061 ccttcctgga cagactctac tgtctcgaaa tggtggatgg cgccagcgcg gatgacaaaa
    45121 aggtcctggc tgctcttgct gtggccacca acttctatgg cggcgttatc gagctgaccg
    45181 gcagtgatgc ctttaagcaa aaagccatgc agctgattgt cgaatacgac atcaaggttc
    45241 gcatgaaact cccggcccag cgggcagcac tggaaaaact ccgcaaggaa aaagagatgg
    45301 cgcatgatgc cattgtcacc cacagaccca cgccggaact caaccgccag tcaccggaag
    45361 agacaacaat ggcccaaccg gctccccctg tgtcaccgaa ggcagaggct gaacaaacag
    45421 caggcagtac ggctgaaaca ccgcatccgg aagcgccggt cacggcaaaa gctgaagccc
    45481 cccgggccga cagaccggaa caccccgccg ccccgtcgtc tgcgccagaa aaagcccctg
    45541 tcagtgcaca gccgcagcaa cccgacacac cggtggaaga gccgcccggc aagctcagcc
    45601 ccggtgaaag tgtgactgcc gtcctgacaa actacggaga aaaagagtat gcgcccggaa
    45661 aagggcgctg cttttttgtg gagctcacca accgcagtgg caaacgggaa tactggggaa
    45721 acggcctgga agaactggtc aaaaaccatc agaagggtga ccccgtcaca ctgacactgc
    45781 aggcgagaga aacattccgg ctgtccggtg aaacgcggga tcgtatccgt aatatctggg
    45841 ccataacccc ggtcagtaac ggcattaccg ttaataatga ccaacctcat gcgggtcagc
    45901 ataccctgga gtttccgatg gcaacattca gccaggtgat gttacagatg caacaacact
    45961 ggccacagat aatgcaggaa gttcgctccc ccaaaaatcc tcccaataat ctgtacattg
    46021 gtgaagacag gcaacctgca tcagcacctc aggatcaatc aatgcgactc cccctcgctg
    46081 gtaatgcccc caatgaactg ataccagcca tcgcctctgc cgataaggac aatcgtcagt
    46141 tgaatcttct gctggtccac agcgcagatg atcatctgca gggcgtggtc cgcctgaacg
    46201 gcacccttta cccggcactg gccaccccgt cagctgacaa ccgtcagctg gttatcaatg
    46261 ccctgacgga taacggtctg cagtttgccg gttacggtga agctgttaac catgatgaga
    46321 ataatcatca gcgtccgtcc ccacaaataa tgcagtttca cctgaagcaa caggactccc
    46381 cgttgtttgc cgccatccac aaaccggaag agcaaccgga caagcttttc cgctctctgg
    46441 gttttgagca gacctggaaa gagtggtctg acagccaaaa ggcggaggac aggcaggaaa
    46501 aaactctgca acaggcacaa tcccacagcc ccggccgatg agccggtaag gacgaccatg
    46561 aacatgaaaa aaacgctgct ggtcatactg accggcatca ccctggcatc accggtggca
    46621 ggtgccgcca cctgccgggc gggggccgct gccgaagccg gcagcagagc cgcctgggag
    46681 cgggcgcgca cagccagcca ggcatggtca cagcgggaga atgacgtcgc ctcctccctg
    46741 cagtcctgcc tgtcccgcat caaggacatc agtatcactc ttccgcagtt ccccagcctg
    46801 gacaagctcc tccgcgacct ggaaaaccag gtctgtgatg ccgtcgtgga tcgggtgaat
    46861 gaccatcttc cggggaatat cgacccctgg aaagactaca acctctgaac aggagcacca
    46921 tgagcgaagc acaacccacc tcccctgtct caccggaagc ggataacagc ggagatattt
    46981 ataaccccgt gctcagaacc atcgcacagt cagaacaacg ggccgcgctg gtacccggtc
    47041 tggtcagggc gttgctgtac agcggcgcag cactctgcat cagcctggtg gctaatggca
    47101 tacagtactg gcatgccacc ggcgtgacgc gcgaatactt tgccactgac aatggccgac
    47161 tggtccgtct ggcaccaacc agccagccgg cctggagtca gagcgacgtc atgaactttg
    47221 gcagcatgac gctgagcgaa gccttcaatc tcgattttgt ccactaccga aaacaaatca
    47281 cgacacaggc cccccgcttt tccgaagccg gctttgccgg ctatgtgaat gcccttcagg
    47341 cctcgaacat tctcgacact attaaaaagg agaaatttaa cctcacaacc actatcgggg
    47401 caggtaccct ggtcaaccag ggacagatgg agaacggcgt ctggttctgg aaattccagt
    47461 atccggtcag gatgcgcctg gtcggtcaga ccagcacccg ccccgaacag gccttcacct
    47521 ttgaaatcac tgtccggcgc gttgaccccc gcatcaaacc cggcggcatg gaaatcagcc
    47581 agatggtttc ccgtaacgcc gcagcaggaa tctgagactc atatcatgca catacatcat
    47641 cttattggcg cactgcttat tgtgtgcggc actgtcacag ctgcagataa cccgggctgg
    47701 aaaaatgcac gaagcccgca gaccggcgga aagaccgcac aggcaaccgg gaacaccccg
    47761 gcaggcggtc ccccgccagc cgcacaactc ccgggaaacc tgccgcctcc cggcgctgct
    47821 cttgtacagg aaggcgcatc gctggattct cccctgaatg ccagtgaaat tcgtgaactc
    47881 cgtgggataa tggccgataa cgaacgggcc atcagtgccc cgctcaccag tgtggtgccc
    47941 cgtatcagtt ccctcacggt gaatctgtct ccgggggcca gcctgcccct tgtccgtacc
    48001 gctctgcata acgtcagcgt ggtgaccttc acggacgtca acggcagtcc gtggccacag
    48061 tcggatccgc cctataacgc tgcgcccggt cactttgacg tgcagttcaa cggcaatatg
    48121 gtgtccgtca cccccctgcg cgcctgggct gccgggaatg tgacggtcta tctcaaagga
    48181 ttgtctgtac cggtcatcct gaatatcacc agtggtgaaa cggacaccac taccctgagt
    48241 caggaaatcg acagtcgtct ggatctgcgc attccccggc agggacctgc cagcccggtg
    48301 gtgtccatac cgtcagaaaa aattgccctg catgacgcca ctctgcaggc ctttctggac
    48361 ggtgtgccac cggatgatcc cgccatccgc cgcctgaaat tctccggtaa cgtcccggac
    48421 accaccatct ggcaacacgg cgatgatctt ctcgtgcgct cacgggccat gctgcgggat
    48481 gaatttgaac agacgctggc atcggctgat ggtacgcggc tgtggaaact gccggtgacc
    48541 cccttactca ccttctccgt gaatggtcag tccgttcacg tcaccccgga actggagtaa
    48601 atcatgagcg cagaacaaga acaggacaca gccgcaaaat ccggcaaaaa gctgtcagcc
    48661 ctgctggtta tggggggcgt gattatcatc gggggcggat acctgctgct cacctggctg
    48721 tcccccgaaa acgctgcatc gctctcgtca gtcaatatca acagcacaac gtccggcggc
    48781 ggacggaacg tgatggagac acagcactac cgggatttac tgcgggctga caatgaacgt
    48841 ggtgctgcag aagcgttacg taataacgag acgtttatcg ccagtctgcc ccggggactg
    48901 gataccgata ccggaaacga tcagaaaagg gataatcccc ccgatgatca gtcaggcact
    48961 tccgggcaaa acgcatcccc ccctcaggaa gatcagactg cccgggaaaa acggcgtgag
    49021 gcattacaga aactgcttgc ccgtatcaat acacagcatc aggatgcccg gccaccggta
    49081 gtggcagcag ccatgtggag caccaccggc actcccggga cagccccaca tgcgtcaccg
    49141 cccggtaccg ttacatcaca acatccccgt ctgcaggaga cgagtctggc aaccccggca
    49201 gcacgtccgg ggatccagct gataccggca ctgacccgcg taccggccta cattgatacc
    49261 gaagtggact ctgacaatgc ggtctcccgt gtggtggcct ccgtacctgc cggcccatgg
    49321 gccggggccg tcctctacag ccaggacgcc agactcgcag gtcagggaat ggatatccac
    49381 tttgaccgga tgatgtggcg gggcatgttt ctgaaggtga atgcatatgc cctgaatgaa
    49441 cgaacaggaa tgtcaaccgt ggccagcgac gtgaaccacc gctggtttaa gcacatcgtt
    49501 ctgccgtccg tacttggcgg ggtgggcaat gtcggaagtc tttacaaaga cgccaacaca
    49561 cagatccttc agggaaacta cggcagtatt accggcaggg tgggaatgcc tgatggagag
    49621 gcgctggccg gcgtcattgc aggtggcatg gccgaacggg gcagccagct gctgacccgc
    49681 caggcagaga gcgaacctta ccgtcaggtg acagtatcac ggggcgaggt cgtctccatt
    49741 ctgtttgttg agccggtcat gagcaacgat atcagtacgc cgtcctccgg cactgcccct
    49801 tctgagtccc cctcctccgg acgtccgctc acccaggcgg aggcagagca ccaggcctct
    49861 gacaggatcc gggcggcggt cctccgtaaa cagaatgaaa tgcagcaacg ctacggcata
    49921 caacaggaaa gttatccatg aaaaacgaac atgaagatcc cggcagcttt ggcgaaacga
    49981 cggcaccggc atccggcaaa tccttcctga aacgccagta tatgggatac agcctgccgg
    50041 tctgggccgg ctgtgccctc atcatcctgg tcgccatctg gtacaagttt ctgcggacac
    50101 cctcagagcc ccctgcagat gcaggctttg ctgtcacacc gcccgctgct gagagctggg
    50161 cagagcccat gaccatgacg gagtccggga gcggaaacaa catgcccgca ccgccggtta
    50221 ccccacaggc agacatgcag caacttgccc gtgatctgaa agcagagctg gacagacggg
    50281 atgaggaaat ccggcgcacc ctgaccatgc tgaaggacag tatcaccacc ctcagtaacg
    50341 ccattcagaa ggatgaggcc tgggcacagg aaacccgtca gttgcttgag gagacccgta
    50401 agcaggtgca taccctgcag atgcagggag cactgccagc cccgaccggg aataacagac
    50461 agcctgcgaa aaccctgcct tcacctctgc agggcatgaa aatcatgtcg gtggagaaag
    50521 gcatggcctg gatccgctgg cagggcagtg cctgggcagt acgggaaggc gacagtctgg
    50581 gaaacatcac catcacccgg attgacccgt ccagccgggc agtcatcacc tctgccggta
    50641 cattacgatg atgagcattg atgccctgac agccatagag aactttgcca gcggtatttt
    50701 cagtgcaggc atggagtttc tctttacctg gggagagctg ctggggatta tcggccttat
    50761 cggtcatctg atgcgcgccc gggcagaagg acgtcacagt atgggaccgg gaaaatttat
    50821 tgccggtatc ttcatctgcg gcatgctggt cagtctgccc tcaatgatca atgccggtgg
    50881 tacccagatg ggcttcaggg cagattcttt cgcccccatc gcatacgtgc agccgggctc
    50941 ctttggtgcc gctgccggtg ccgtcaatgc cattctctca ctggcaaaac tggccggcgt
    51001 cggttttgtc atgaacggca tatccatctg gcgcaaatca ctgctggacg ggcacaccgc
    51061 cctgagcgcc agtgaatcca tcagtaaagg gaacgtcaag tttgtggccg gcgttctgct
    51121 ggttttcaac gaccgggtta tcgatgccac tctggcaagc ctcggtatcg ccttctgacc
    51181 cctgtatttt ttcagtaaat caatctgtta ataaaggaga cactatgtca cgacttcgtg
    51241 ccatggcggc gcgtctgcgc gctgcatctg tttatgttca gttatgcctg atggcctgcc
    51301 gtgaccggct ggtacgcctg ttactgctgc ttcccgtgat gctgctgccc cacaccgcaa
    51361 tggcaaatac cctcttcgat atgattaaaa ccgttggcga tggtgccaga ggctccagca
    51421 gttcgtttgt ggctatcggt caggttattg gcctggtgct ctttatcggc ggactgcttg
    51481 gcctcaaaca aatcggtaaa aaacccggtg cagggctggc ctgcctgctg tcaattctgg
    51541 tcggcgtctg ccttgccgtc gtaccggaac tgatgggcag cggacagaaa acgctcggtc
    51601 tttctccggt ttcaatcagt taatccgcaa aggcaccggg aaaccggtgc cgtcttcaca
    51661 caatgaggag aaccagacca tgtcctgtga gctaccccct ttcaccctgc tcagaacaga
    51721 gcacggcaac gtgattgccc gcgacaaccg ggataatcag gagtttgtgt ttgcagagtg
    51781 ttactccacc gctatagcag cactgattct ggctgccgtc accctgtata cagagagaaa
    51841 agcgtatgaa aaagaccccg ccactcatcg ccccggtccg ggaacgatgg cttttcccgc
    51901 tcccgcaacc ggacacggta ttcagcggtg aagccctgtt accaccgcca gtgctctcaa
    51961 cagagggacg ctgcccctgc tgtcgcagga ccgtcaccca ccgcttcatt ctggaagatt
    52021 catggccatt acagcagatg gctgacacct gccgggatac gatcgtcctg ctggaaaaaa
    52081 acctcacccg ggtgatgcgc ctgaaaaaac gtccggtacc ggaaaacgct gacgaaaaaa
    52141 agaaacacac ccgaaccctg caggatgcgg agcggtccct ggcacaggcc cgcctgtcgg
    52201 ccagacgact ggccctgcgt catgtggaaa agtcgcaaat cgtcacaacg gatgcactca
    52261 gcgaaaatga aagtgacctg ctgcagccgg aaggcccgcc ctttcatctc tgtgccttct
    52321 gccacgcctg gcactgtctg aacggctacg ctgccgcaca gggagtgatg gtctggctcc
    52381 ccgatttgca tccggcctcg gtggttgccc tcaatgcgcg ggcactgcag gagattttca
    52441 gcgataaccg tcagcgggtc aggcagggac gggcagtcct gaatgccctg gtacagaacc
    52501 gtctggccgt tgaggagaaa ttccgcacct ggcgtccggc ggattttgct gatgccctgc
    52561 gccgctggcc gccggcacag cgtaaaacgt tgagggagaa aatggacggg gtggcgctca
    52621 ttctgctgcc ggattcgttt cctgacaaaa agtacgtcat gtgacacaca ccggtgccgg
    52681 tgacgttttg ccggatatca gttctccgca gatgcattcc ggcactttat tatccggtcg
    52741 ctgactgacg atattccgcc tgacggagat gaaaaagaga ttgcccggtt cattcgccgc
    52801 cagcagttta tttatccggg tgtaatcaca cagaaatacg cccctgcccc tcaacatatt
    52861 atttatcttc tctgctgttt tttcatgcca catcccctgc tgtggcatca aaaaatataa
    52921 gaccgtgatg attcatcctt accggagtgc cccatgccat gaatatcaac cgcattattt
    52981 ttgccattga agactgtatc agtaccctgt cacggtattc cgtttccgca ttcttccccg
    53041 attactgtga tctgcacacc gtcatcggtc tggaccagca ggaccgcgaa cgccgcccct
    53101 ggctgaaagc cccctacatt gccaccacaa aacaggggta ttacctttcc gtctttgaaa
    53161 tcagcggtgc cctgaaagaa atggatgaaa acccggacca gacggccccc gggacactgg
    53221 agtcactgat tgcagcactg gctgagcgaa tgaacacggc ctggaaaaat tccggccaca
    53281 aaatcagttt tgttttcgaa cgcgatccgg agcgtggaag ggatgaaatc gaagccatgc
    53341 tcttacccca gcgaaaatcc atcgcacgca cggggatcct gcttcaggac gtcctggatg
    53401 aaaaagtcac cacactcacc ccctggcttg tccgggagcg ctgctggctg accgtctgga
    53461 gctctgaaga actgctgagc cggaccgatc gcaagaacca tcagacccgt gtaaggaaac
    53521 tcgctgaaca cgctccgcca gcccgttttg cgcaggatcc ctggcgctgg acactttccg
    53581 ccctcaaaat acggcatgac gccttactgg acacgctgga gcaggctctg acacatgatt
    53641 ctgatggcct gctgattcgc ctgatggata tccatgaagt gggacgggaa atccgccgcc
    53701 aactggagcg caacagtaca ccggcagtat ggcagcctca tctgcccgaa gatgcccgtc
    53761 ctgccggctg gcgacagggc ggggatacct ctgtattcca cgcaccgtcg ctgaatctgc
    53821 agttattcac cacacagcct gaaacacacg gcagcctgat tcaggccggt gagctctggc
    53881 acggtatggt cgccattacc ctgccccctc agaatctgcg gacattcaac cagctggtca
    53941 gggatgtgcc ccgtgcaatc ccctggcgta tccggatgga tctgatgccc gacggcatga
    54001 aggcgctggg ggtcaagaaa accctgctga cctacagcag ctttattcct cctctccggc
    54061 ccatgtatga ctcggtaatg atgctgaatg acatcaacaa gcatgatccg gtctgtatca
    54121 tgaccattgt tgccaccaca tggggaaaca gccgggaagt ctgtacccgt aaccaggctc
    54181 tcctgaaatc tgcgctggaa ggctgggggg tatgcggcac caccacaacc ttcggtgatc
    54241 cgagacgtgc ctgggtgaac acggttctgg cagcctccca cagctccggc ccgatccccc
    54301 tttacccgcc cctttcacac gccctttccc tgtttcccct gaacagggca ggctctgtct
    54361 ggcggggaca gggaaatctg atgatgcaca ccgaagacgg ctctgcatgg gaggtggcgc
    54421 tggcgtcatc acaacagaac aagcacacgg agctgacacc cggcgcaccc ggtctgggca
    54481 aatcggtact catcaacgca ctgtcggaga tccagatagc ctcagcccag aaaaatctcc
    54541 ctttcattgc ctatatcgac aagggattca gtgcacaggg actggtacag cttatccgtg
    54601 acagcctgcc tgagcagaga aaggacgaag ccgtggggat cattctgagt aatgacccgg
    54661 accacacccg caacctgttt gatgtgatgt acggggccag aaaacccgtc acaccggaaa
    54721 agaacttcat ggtctccgtc ctctgtgcat tgtgtgtgga taccggcacc ggtcagccct
    54781 gtaatcccgg cgacacccgg caaatcatca gcagcctggt ggacctggcc ttccgggaat
    54841 atggcgagaa taaccccaga ctttaccgcg caggtacgga acccctggtg gatctcgccc
    54901 tggaagagag cggcatcgct gaacaacatg atgccggatg gtggaatgcc gccacctggt
    54961 ttgaaatcag ggacatgctg catattgcag ggaatatccc cgccgcccag cgggcacact
    55021 accaggccat gcccctgctg gctgaaatgt ccgccctgct gggacagccg tccatccggg
    55081 atgtgttcgg caccgttcag cgtgataact cggaagagcg gttgctggac tacatccgcc
    55141 gtgccctcga tcagggacac agtgactacc cgatgatgtc cggctgtacc cggtttatgc
    55201 tcagccccga cacccgcgtg gtcgccgttg atctgaacaa tgtggccggg gacaagacac
    55261 ctgccggacg tctgcgcacc ggtatcatgt atctgctggc cgggcagatc gccggcgggg
    55321 attttgtcct gccgcagtat caggaggaaa tacggaaaaa tctcgaccca cgctaccatg
    55381 aggtgatttt ccgccgcatt gaacagcttg atcaggaggt caagaccaaa gtctatgacg
    55441 aactgcacaa tgcaaaaggg atcaacttca tctgggaagc cctggacacc caggagctgg
    55501 agcagcgtaa gttcgggata cgcacggtgc tgagcaccca gctgctgcag cattatcctc
    55561 cggccgtgct caaatccgcc aacaccctgt ggctgctgcg ttaccgccct gacgaaattc
    55621 cgttcctgcg ggataacttt ggcgtcccgg aagtcacact gcggcgcttc ctgaaaatgc
    55681 cggaaggggc cgcaccggac ggcagcggtg tcccggtact ggctgtgttc cgtgtaaaaa
    55741 acggcacgct ggcccggatc ctgaaattca ccctcggtcc actggagctg tgggcactga
    55801 actcctctcc taaagacagc gccctgcgac gtgcgctgac gcaggaggtg gggtcactgc
    55861 gggcacgcca gatcctggcc gaacactttc cccggggttc agccacctca ctgatagaac
    55921 accgcgcccg cacacatgac agtgaaaacg tcatccatga gctggcagca gaactcatcc
    55981 gtaagcaggg atataacctc tgatgtacag cattcatatc accccccgct gccggcgtcc
    56041 tgcccgggga caggtcttcc tgaaaacact gcgcgtggac ggtgacagtt tcagcttcac
    56101 cctgccgtcc gcctgcttca cactgcgacc gtcccccaat ccccgggtca tttttgccag
    56161 tgacagacgg gctgacgggg agatgagtgg tattcgcctg gccctgcccg gagagacagg
    56221 cacgctgata acaacggcca cctggcaggt taacgtgccg gagcgaccgc cttttgatat
    56281 acagcatgtc attcactggc ttctgcccgc accactcact gcgctgatgt ctgacaacac
    56341 ctggctgtgg ccggatgtcc ccggcacacc ggcactcaca gccggagact ggccggtcat
    56401 ggaaagcgac accccgacac tgaaacgcgt gaagcgtcgt accttcacgc tcatcgataa
    56461 ccgtaaccgt cagggatgga tcacgcgccg cttccagtgt ttcccgctgg ctccgctgcc
    56521 ggaatcactc agtcatcgcg taccagacat cctgtatccc ccgaaaacgg agaaaaaaga
    56581 atgcaaagac aatctgcaac cacactgctt gcactccttg taggctgtgc ggttatccct
    56641 gccacccatg cctactctgt gaatgttgtg tccagtcagc cggtcacccg acagatcatg
    56701 ccggagctgg ccaccataca gggcatcatg gaggaaatgg tccgtatgca gacggccacc
    56761 ggcacagcca tcacccagaa ctcagaaaaa ctggccaccg tcattgccca ggacgggcag
    56821 gccacccgtc agcagatgat attcagtaat gaaacacacc gcctcgagga ggccaggaaa
    56881 agcttcagcg tgccggactc catctgcagt gaatccgcgt ccggcattgc agcagaaagc
    56941 cgccgggcag cagcgtctgc agcggcacgt ctgagtcagg ggggaggggt cagcagtaaa
    57001 cccatccggg aacgtctctc ccgggctgcg gactccccgg tccgcgaggc gtatgacagt
    57061 gccggcatcc acgccggtta ctgcacggaa gcggagtatg cccgctttgg cggaactgat
    57121 gtctgccctg ccgtgggcga tctgccgggc ggagacagcc aggtacgatc gctctatcag
    57181 ggcgcaggca cagcagatac cccggccgct ctcacctggg atcagaaaca gattgatgcc
    57241 gcgacggctt atatgaaaaa caccgccaga ccgtcagcgg gtcgtgcccc ggggaaagga
    57301 gaagtgggca cacagaccgg gcgcacgtat gtgggcctgc agaatgaata caacggcatc
    57361 atcgatgccg cctctcatcc gcagctgtca ctgattgctg acagtacccc taatgaagcc
    57421 acccgtgggg cactgactga agccctgcag tcaccgtcag ctgcggcata ttttgaccgg
    57481 accgcatcat cagaagcccg cacccgtggc cacatgagcc agcgggagtt tgaggccttt
    57541 gaagccggca gacggtatgc caacaccgac tggcagcagg atctgcaggg catggaaggc
    57601 gataaccttc tccgtgagct gctgcgcacc accgccctgc tgaactggca gatgaacgat
    57661 ctgaaagagc agattcgtca gggtaacgtt atcgccgggc agcaacttgc gctggccgca
    57721 cggcagtatt acggacagcg ccttggcgaa ctgtcacagg ccatgagcca ggggagcgta
    57781 agatgaaaga cgacaacagc atcgacgaaa aaaaaccggc cccccgtctc cgccgcatcg
    57841 gtgatatagg gataaatctg ttcgcaccac tggccgaaac ccgctggatg ctgcgcagct
    57901 gccgccatat gctggaccag cataaaacgc gcctgaagcg cctcatgccc gaccggcaac
    57961 cggagagtcc ggtctgtatg acctgggagg aagcggtcag ggccagcgga ctgacggtcg
    58021 acgaactgga ccggcgtctg cgacgtcgca ggatgttctg gcggacagga ttctggctga
    58081 tgatgatacc ggtacttctg ctgtgtggta tggcccttgc ggccaccgca ctgccggtca
    58141 tcaccctgat acgtatcctg gtctgcctgt tactgatgag taccattgca gcactgtgtg
    58201 cctcaaaagc cctgattgcg tcataccgcc tctggcagct gaatgaacgt cgggtcagcg
    58261 aggacgaaca gggcacattc cccgattacc tgcaggcccg gcacggctgg aaacaggcca
    58321 catggctggc cattacccac cggcagtact gactgtccgg tctgcaattt cttttcctca
    58381 ttccgggggc tgatgcccgg gcatcccctt cggttccgga gttaccgtga aaatcatacc
    58441 cagagcactg agtgcaggtc tggcgctggc tgccgtacct gcacaggctt tcgtcaccta
    58501 tcaggacatt gtgagtgccg ccaccaatcc ggatgacctg tcgcgccagg cactggtcac
    58561 catttttggt gatgtggtca caaatcccct gacagccagc agcaacacca tcattggcaa
    58621 cctgttcatg gtgtttaacg caattatcgc cacgatgggg atagtctggt ttctgtttat
    58681 tggtatccgt catgctgtcc gcacgggaca ccagggcagt gtctttaaca acggacggga
    58741 cgtcgtcggc atactcagtg tcgtgagtgg atttttgatg attgtgccca ccgcttccgg
    58801 ctggagtctg gcgcaactgg tcatgctgtg gggcgcatcc atcatgggca tcggttccgc
    58861 caatatcatg gttcagacag cggcagaaaa cattgctgaa ggctattcaa tgaccgtaca
    58921 gccagtacat gtctccaccc gcacggcagc acggggaatt ctggagatgg aactgtgcaa
    58981 acacgccatg aatacgggcc tcgatgactt taaccgtacc gcacgttcat caacgccgcc
    59041 catgagcgaa tcctcacgga cagagaatgg cagatatacc gtcaccgtct caaatggcag
    59101 cgccacctgc gggagtgtga gtctctccgt gaaaggaaac ggcagtaaca agcagtcctc
    59161 gctggcccat tttttcaatc ccttcattga aacggaatat cagggggttc tgacggcaca
    59221 acgctccgcc atggatacca tgctccgtga cctggacagc gcggcgcagg aatttgtgtc
    59281 ggcataccgg acccggcgcg acacgggtaa cggcaacctg ccggatattg aaagccgtat
    59341 tcagaaggca gccaaccggt atgaagaaac ggtgcagaag gcgttgccgg cagataacgc
    59401 agaaactgag cgcagggagc agctgaaatc ctacctgacc acctacggct gggttgccct
    59461 gggtgcctgg taccagacct ttgccaccgc caaccagcga ctggaagacc tggccaaccg
    59521 cgctcctact gtgagtggcg tctcgtcact gggcgaagcc ggggatacgg aattcctgaa
    59581 ggaagtcaag ggggcttacc aggcacagct acagaactca gcgtatactc ctgcactggg
    59641 aacagccacc tctgccagtg aataccacgc atctaaaact caagaccctc aatcagcaat
    59701 cttaaaattg attgggttta acctaacaag aatgactaac tggacagcca cttatctgag
    59761 cggtacaggt accacaacta atcaactaaa tccgttgata aaaatgaaaa acattggcga
    59821 ttacaccatg gttacgaccg aaaccatctg ggcgggttat accaccgtca gggtactttc
    59881 cacggccggc agcaactccg tttttggtcg actggtcaat gccgtcagcg gattatctga
    59941 cgccgcaaag gcttttctgg aagcatccgc accaccactc tacttcctgc tgtttttgct
    60001 gttttgtgcc ggtttttcgt tatccattta cctgccgttt atcccgttta tcttctggat
    60061 gaccggcatc ggcaactgga tagtcagcgt actgattgga tgtgctgctg gccccctgtg
    60121 ggccgccacc catctgggaa caagtcagga ccggggaagc cgggccgctt acggttacat
    60181 ctacctgata gacagcatga tcagaccgct gctgatggtg ttcgggttct tttttgcatc
    60241 agtcgccatc gtggccggcg gcacaatcct gaatgcgctg tatgctgctg cactgatgaa
    60301 tgtccagata aactctgtga cgggcgtgtt cagcatggcg ggatttttga tgatttatgc
    60361 ccgcgcctgt accacactgg ttgcagccgt ttttgcgctt caggcttatc tgcctgacca
    60421 tgtaattaat tttctgggcg gacgtgacgg tgttaatatg ctggggaata tggtttcttc
    60481 agtcaaagat attattgccg gaggtaatac caatatgcgc agaacacctg gcgttcgtga
    60541 agaaaaactg aaaaatatta atagagatga taaagacgga ataaaacaat aaatgaaaat
    60601 atcgaaaaac cactaaaaca ccattgccat attcacctga agagattaaa atacattcag
    60661 gtgaatatgg aggtttatta tgaacacaac aaaaagcaga tatccttcct ggtgggcata
    60721 ttattacctt atccgcactg cttattttat atttggtgtt ccttttttat tactatgttc
    60781 tctaggattt accggtatga tgcttacaga accatatata gaaacagata ttcaagcata
    60841 tctatatact tatggcagtt ggctcctatt aatactccca tcactctggc tatatatgaa
    60901 aaccagagcg aagaaaaaga aagtccagca ggttgttcag cagataaaag aaagcagtcc
    60961 ctttgcgccg accagcgatt acgagcaact gtcattctca aagagttgtt atttcgggat
    61021 cgacattaaa aacggcacca tgctgtatgt gcgcatttat ccgaacaatg tgatggatgt
    61081 gattggtctg gatattcaca actttacccg cacggttgct gaggacggca agctggaaat
    61141 ccacaccacc tatgtgagtc tgccgatgat tcctcttgag atctccggca tctcagcaag
    61201 gacgctggcc aacaccatgc acaccatggc tgcacgtgga tatgagtaca aggaacgttt
    61261 cccacagatg atccggtatc gggttaaaga gtgggaaaaa gttgccggtg ttcctgttgc
    61321 agaagtattt tgaggtagct atcaactgtt agacgataat ccgtcagccg gttttctctg
    61381 aaccggcttt ttcttttcag gcagaatgcc tgtctgccgt ctatctcagg acgccgcatg
    61441 gagtctctat gttttcccca caaattctta gcactgtctt gcccactgta gacaaaatca
    61501 atacaattct gcatatcagt tatctataaa agataacaat atcattataa ataattatca
    61561 tatccacaca ctcaaggcag tacctagaaa acataaagat aaaatcatgt ctgaatcccg
    61621 cagcaatttt ccagcacgac atggtactcc atggaatact gaagagttac agcgtcttct
    61681 cgtatccata gcggagggcg tctcgctaaa aaaacttact caaacacatc aacgatcgtc
    61741 cgggggaata cgtggagcaa tcaaacgcct gcttccttct gagtttcttg agttcaacga
    61801 tataaacacg gtgaatgatc tcagcaatta cttcaacaag attcctcgcc aagaacacaa
    61861 tagtatcatc aataatttaa tatcattgag cactgcaaaa acaattgctc atgataaagt
    61921 tgaaatacaa aagaatagtt ttaaacaaaa taccccactc cccctctgcc aacccaatac
    61981 agattttaaa gattatatag ccgatgaaca tgatgtcatt atgctaattc attctgctat
    62041 cgaaagttta tctaataaac gacatcaata tattcttcta atgcgactcg gcacagatga
    62101 ttcacctcat tctctggcag atattgccgt caaattagga atttcccgtg aaagggtaag
    62161 acaacttcag gaacgagccc ttggcttact agctgctaaa tctcgctatg aagggacacc
    62221 tggagcttgc cttaaagccc ttattaactc aataggtaat tcaccatatg atatagcagt
    62281 atggatttat cgtattgttc atcgggattt cgttacatcc tctgacttag ccgccaaatt
    62341 cattattttt accgcaggca ttcctaaagc aagacgagat gaaataaaaa gttcgctctc
    62401 attaatatca caattacaaa agcagaggtt acgtgaacga agagcagaat tagcacacgc
    62461 tcgcgctcag gaaagagccc gaatcaagaa agaaagagtt gagttcatat tcagtaaatg
    62521 gctagacaat acacattggc ccaaatatat cgcacctcct ccccccgtag aacagctatg
    62581 ttcacagaga gcggttaatg atactgacat atctggattc ttttactctc aaaaattagg
    62641 tcgtacagtc cagtacgaat cagggcttga gtttaaaata atgaatctat tagaatgttg
    62701 cgatcaggtt cttttctatc aagaacaacc attttctatt ccatattgtt ttaaaaacaa
    62761 atcaaaaaaa tactatccag atttactcat tgcgacagtt gatggtcggt gcctacttat
    62821 ggaagtgaag ccaacagacc gaatgacctt gagtctcacg agaataaaaa gtaatgcagg
    62881 tcgcaaatgg gcacatactc gaggatgggg atggttggtc attagtgata ggttctcgtt
    62941 acgtcagctg gaagaacatg ccatctcaac aaacacatgg aaacttatag aggctgaact
    63001 gaaaacatca aggattttag catggcgtga aatgatggag ttacgtacac gatacgagat
    63061 tcatcgttta gatcttattg catatatcat tcagtctggt gctgaagtgg ataactttta
    63121 tcgaattacc ccgaaaatat ctaataatac atctaaccag agacaaccaa atgagagcga
    63181 ctgtcataat tgatgacgca caggacggaa aaaataatag ctgccggaca gcagcaacca
    63241 gcagtcgtcc ggtaaaaaag acaaacaagg cgcattgagg tcagccttcg caacaatccg
    63301 gcggcagatg tcctgccgcc gtgttgcccg ttaacgttta acttcgtagg ctaacgttgc
    63361 cacaagcacc gtatttccct gctcgatacg aaatccgcaa agcgaatccc tcaccatcca
    63421 gacaaaacac agtatcgtca cacagacgac gattaacatc attaaaaacg ttcgttgtgg
    63481 catcgcagcc cctccattgc ctttcggccg tcatcgggct actataatgt tgtctaggca
    63541 ttacagtggg ccctgggttg attgataaaa aaatcgcctg gggctttctg ctacatgccg
    63601 tccacaaaac gctttcggca tacagcctca agcgcccgcc gctattctaa tcgttttcct
    63661 gtccgccaca atccttttca tgccaccacg tattttgtgt cagggacaga aataaagccg
    63721 gaaagtgact acgtgtgcac acatctgttt tccgggctgc aggcatcgtg ttattttctg
    63781 aaatgcaaaa taataaaaaa caaggacaca tcatgaagga tgaaatacac agtaccagca
    63841 ttaacccgga tgttgttgag cagtttctgg cccttatgaa cacacattgt aaatcctcag
    63901 aagaggcact cgccatgctg agtacgcttt gttacaacct gatgatgaca tgcggtatcc
    63961 attccatgga cagccagatg ctcaatgggc atgggatatc catgcaactt caggggattg
    64021 atattgccga aaggccaggc aagaatattc actaacctga acagggtctg aaaacgctga
    64081 tttttcaggt ctctgcaggc taaaactgat gatgtaatga caacaaaatt tctgcgtttg
    64141 taccccaccg atgaaaaaga caaaaactca caccgggctt ctcatcatta aagataaaac
    64201 ccgacgtgtc agcctctacg aaacgccaac ggcatggtgt atcagggggc aggagtgcta
    64261 cagcaaaagc actggacgcc gctgtggcag ccacgacagt ctgagcagac tgcgcctgga
    64321 cagcattaag cctgttgaat gagtggtggc accatgaaag ataacaacac aaccgccacc
    64381 ccagaagcct ggaaaatgcg ttttctggcc aacgggacat ccggcacatg gttttacact
    64441 gacgacattg cagtcactga cgcgtggctt cgcgcgcacc cggatgatgt ggaggttatc
    64501 cctctctgcc ggatacagcc ccgccagcat tcccatgaac aacatattga agaactccgc
    64561 gcagccctgg cccggatgct ccatatgcat catatgatgc tgaaaaccac acattaccgg
    64621 gaaaatatca ccgatgcccg ttgtatccgt gaaatgcatg aagcgccatt acaggcgctc
    64681 aatattctgt ccagataccc gcataaaaag gacgaggtgt gacgcatgtg gctactgatt
    64741 ttctggcttt catcgccctt taatgtctct tccggtgatg cccgtcagcc cacactggcg
    64801 gtgcacagcc aggcctttca taccgaacgg gcctgccgcg atgcattgcg cgccattaaa
    64861 aaggagaatg ttggcgaatt ttctctgcgg ggcgtttgtg tttctgacgg aaatacgaag
    64921 gaggaggctg agagctcatg acggatatta ctgacagaca tacacttcgc cgtatgtcct
    64981 ggtcggaact catcacggca ggacaggaag cagaatttca gcgtgattac gagcgtgcca
    65041 gaatagtgtg gtcattcgcc ttacatgttg caacaaccac gattaacaaa aatctggcca
    65101 tagctcatat tcgacgctgc gatactttgt tgcataaatc aaaggctgta cccggaaaca
    65161 ccccgtgtgg ttccggtcag cgagtctgag acctcaacat cccgggtgat gattgtcatt
    65221 ccggactgac gcagccgctg cacatctgcg gcaatacgtt gaataccaag ccagaacaga
    65281 ccatccagcg cggtgacaat taacccgctt tccagcgcca gtttcagccg tgctgtcggg
    65341 gctttaacct gcccggctat atcagtgaaa caacgcgtcg tcatgccata gctgtccacc
    65401 cgccgtattc gcgtcgcaag actgtaactg aagctatccg gcacgccgga taagtcattc
    65461 tgcagactgt atacacatcc ccgctgccgc tcattgaata cgactttttt ctggctgagc
    65521 tgccattccc gccgcacgct gtcaatcatc tgcggtgtcc gggttaacag cagacgaaac
    65581 ggcagttcaa aactggtcac ataatcaaca ttaagtaagg caatccgcag ccgctccctg
    65641 gctcctgctc cggtatcacg gcattcacgc atgacatctt cccactggcg catcagtcgc
    65701 ggtgactcat tcctggccct gaactgatat ttctcaatct gataatcaat atgttcctgc
    65761 ataccgtcac cggatatctg ttgccgtatc atcacggata tcagcgtact ggaattcatg
    65821 ccctgcgtga agtatcacac tgccaaaaaa aacacacctg aacgttatta tttcgcaata
    65881 ttaattgcac ctgttttcat aaataaaaag gagacgatga aatgataaaa tatatctttt
    65941 cactcaccat tattttttcc gcttctctct gtgccgcaca gattcagggg cacgtcatcc
    66001 gcgttcttga tggtgatacg ctggaaatat tacaggacaa aaagcccgtc agggtccgtc
    66061 tggctaatat tgatgcaccg gaaaagaaac agccctttgg acgctggtca gcctctcagc
    66121 tgaaggacct ggtggccgga aagcccgtaa ccgtgcaata cgcgcagaca gacaggtatg
    66181 gccgtattct cggccgggtc tataccgctg acggcacaga agcaaatcgc tggcaggtcc
    66241 gtaacggcgc tgcctgggtg tacccggact acaataatga ctatactctc ccgccgctcc
    66301 agcgtgatgc ccgcgccctg aagcggggat tatgggcaga taaaaatccg gtaccaccat
    66361 ggacctggag aaaacagaat aagaaataat tatttgctga tatgaagaaa aaaaccattt
    66421 caaagaaaat aaatggcaaa aatacagcat ggaaaaaaat tgccattttt accgccgcgt
    66481 tatttttaat atttgctgtc tgctggtcgt aataagcaga ataacagaaa ggtgtaaaaa
    66541 tgaacctggt tgatgcattt gtcactaaag tgatatccgg accgtatgaa gaatacggaa
    66601 aatggtgggt ggatgtagaa tatatgtcct ggggagttcc gggtaaaagc cggctcatgt
    66661 ttgactcacg ggaacaggca caggaagtgc aggaaggttt tcgattttta acctgagcat
    66721 attttgttaa agggcagaag gcggtgcccc tctgccctta agtacgtttc cgggattata
    66781 agtcaaccag tccccggatc agttccagga gagctatcac tgcgttagcc acagcaacaa
    66841 ccgccctgag aacgatgatt aacaggttaa tcatccgctt ctccatcaat ggagaaagaa
    66901 caccgtggtt tgcccttaca ttgcctgtcg ccacgttttc tatagtgtca gttatagaca
    66961 gttcttctcc ggccagagca ccaaaaccag caccacctga tgttttggcc aggacttgca
    67021 acctgttcat cccggtgaac gatatgccgg aaagaacgtg cctcaaagct gacacaccga
    67081 gtatatactt atactgtgct tgcagaaata ttatttcagc cgtattattt catagtcagt
    67141 tataggcagt tatcttccgg tcagggctac caacttcgat ccgaaatcgc aaaaaaaccg
    67201 gcctcaccag tcggtttttt tgcgtctgta cctcctcccc ggacagcagt tctctcactc
    67261 ccgttgaata tttcctgttg aattttacga aaatacggca aatggattga tgcgaaggta
    67321 agtctccaga caaggctaac ttgctgatta tacttgtttc aggcgatatc gctactcccg
    67381 gaacaataga ctgtaacgaa cagattgcga tcaggtgaat ataacgatat tctggtaacg
    67441 ttagttgaaa agagacttcc tccgggccag agccacccac tctccctgga atcgcaaaaa
    67501 agccggcctc accagtcggc ttttttgtgc ctgtctctca ccaccttctg ctgttcctgt
    67561 gaaaatgccc tgctgaattc agccctgccc ggtcattacc ggcgattgtt cgcattaatt
    67621 ccactcagcc tcatcccgaa atttatctat ttatcgatgg aggaatcctc gtgtcttacc
    67681 aacactcccc cacaacagaa gacccgatga acatatggta tatcgttggc accatatgtc
    67741 tgctctttgc gcttctcatc tggctctttt tacccgacat cgtctttgcc acctgcctga
    67801 tcctgcacac aatgtggggg atgactgact gggggccttt tcaccatttt gccgcccccc
    67861 gttataacct gctggccaac accgccaaca acgctgcaac gatcactttc agccagtggc
    67921 tggatgtcat gtcacagacc gtggggatcc tctggctgat tctgcttccc atgacgatcg
    67981 gctttctgtg gatgtggttt cagcatccgg cccagccccg cttcacccgc agaccactga
    68041 atatccacac gctgccacat ctgttttcat cactgtcacc ggcgatcgcc cccgttctgg
    68101 ctgacggaga cagcaacaga ctcttccacg gacaaaaacg tcccgagcgc cgtgtcgctc
    68161 taacccccga agcctttgtg gagcagaaca accttatccg caacatgcag ctcgatgttg
    68221 cggccacgcg ccagtgcttt atggcgcaac tgggccagcc tctgaccacc tggaaagaca
    68281 tggctccaca tgaaaaagca ctctttgccg tctttggcct gcagttcttc cttggggacc
    68341 ggaaatcggc tgtttccctg atgaataacc tcaacctctc ctgcagactc aaaagcaagc
    68401 gggaccaggg gcgtttcagc acgccagtgt attcactggc cagaaacgcc tttatccggg
    68461 tcataaaaac agaagacgca caacagtggc tcaggcagca ccgttacgtt cgcagcggtc
    68521 ttgtctggct ttacgcacat gatttgcgcc tgacgccccc caactggctg tggctcaagg
    68581 gcgttgaccg gacgctcttc tatgcccttc accgggcaaa caccacaaaa ggcttcatcg
    68641 aaggggccgg cgtggtggcc gttgcccggg cagaaaatga agccagccgt ctcggcctcc
    68701 cctgcccgga accctgtgtg gaagaagcca ttgaagggct tcgacgggac atgctggggc
    68761 tgggcctgat atgggacgaa cctcagccag atcgcgatcg taaacgtcag atcaggaccc
    68821 gctggtcact cacggatgac gtgatacccc gccgccatga taacgacgaa gacactgaca
    68881 ctggtgaaac caccgaaacc accgaaacca ccgaaaccac cgaaaccacc gaaaccaccg
    68941 aaaccaccga aaccaccgaa accaccgaaa ccaccgaaaa acagcatccc gccgacaagg
    69001 aaaaagaaca atgaccatcc actacactgc aacactgaat aataaacacc tgtccctgac
    69061 cgccctgtac cctgacggta atcgggaggt gattgccatc ctgccagtca cagaactggg
    69121 cgcactgatt aaccgtaata acaggctgat gcacctgtcc ttcagttccc cttttcgcga
    69181 aataaccgaa acggtgcttg ttgataccgt tgatgcagaa cgtcccggac atcacaccgg
    69241 cagaatgctc tcagcgattg atgcctgcct ggatgccgaa tatgccggaa aaggaaaaca
    69301 tccctcccgg aaaaatggct tctggcggac actgatgtgt ggctttatcg ccggaattgc
    69361 tctgccggcg acagttgcag gttaccactg gatgaaggca gagccccctt ccacgctggc
    69421 ttaccctaca tctcaggaat ggagagctga accagtaacc gacacagaga caccacgccc
    69481 acatacggaa catcatgtta acagcatccc ggcccgcccg gctcccacac agcaggaacc
    69541 ttcttctgcc cccgctccga cggcagtgac ggaagaacaa cacgttcagg ccgctgaggc
    69601 ccgccgtaat ctggccacag tacttaaacg caacgcagac cgggggatgt tcaccattaa
    69661 cctgtccact ggtcatgaac gcacgctgta cgccttcctt gaccccgcat gcatcaactg
    69721 ccgtctgctg gaacccgcgc ttaagcaact ggcccgggaa ttcagcgtgg tgatttatcc
    69781 ggtctccgtg attggcgggg agtccagtac aaagcggact gctccgctgc tgtgtgaacc
    69841 ggactcacaa aaacgggcga ccggatggca tcagctgtat tccgctgaca gtggtatggc
    69901 ccccaccggc gatgcaccgg ccgcacagga tggacagtgt ctgaccgccg cacgtgctgc
    69961 catcgatgtc aataaccagg ctttccgcca gttcggtttt ggcggaaccc cctgggtgtt
    70021 atcagatacc ggctggcacc tgccgtccgg catcctgaaa gacgccactt ccgttcgcct
    70081 cttcctgaaa gcaacagata gcgaggagta tcacaatgag ccagcaccac gttaatcctg
    70141 acctcataca ccgcacggcc tggggaaacc cgttgtggaa tgcactgcat aacctgaaca
    70201 tcactggtct gtgtctggcc ggcagtatca ttacggcgct gatatggcca ctggcccttc
    70261 ctgtctgcct gctgttcacc ctggtcacct ccgtgatatt catgctgcaa cgctggcgat
    70321 gccccctgcg tatgcccatg acactcagcc ttgatgaccc ctcccaggac agaaaggtca
    70381 ggagaagcct cttcagcttc tggcccaccc tgtttcagta tgaagcggat gaaacattcc
    70441 ccgcccgggg gattttttat gtcggttacc gccgtatcaa cgatatcggt cgtgagctct
    70501 ggctgagcat ggatgacctc acccggcatg tcatgttttt tgccaccacc ggtggcggta
    70561 aaacagaaac cacctttgcc tggctgctca acccgctctg ctggggaaga ggctttacct
    70621 ttgttgacgg aaaggcacag aacgatacca cgaggacaat ctggtatctc tcccgacgtt
    70681 ttggcaggga ggatgatatc gaagtcatca acttcatgaa cggcggaaaa tcccgcagtg
    70741 aaatcattca gagcggcgaa aaatcccgcc cacagtcaaa cacctggaac ccgttcgcat
    70801 tcagcaccga agccttcacc gccgaaacca tgcagtccat gctgccccag aacgttcagg
    70861 gcggtgaatg gcagtcacgg gcgatagcca tgaacaaggc cctggtcttt ggcacaaaat
    70921 tctggtgcgt ccgggaaggt aaaaccatgt ccctgcagat gttacgggaa catatgacgc
    70981 tggaagggat ggcaaaactc tattgccgtg gccttgatga ccagtggccg gaagaagcta
    71041 tcgcaccact gcgaaactac ctgcaggatg tcccgggatt cgatatgtca ctggtgagaa
    71101 cgccgtcagc ctggactgag gagccccgta aacagcatgc ttatctctcc ggtcagttct
    71161 cggaaacgtt cacaaccttt gccgaaacct tcggggacgt ctttgcggca gatgccggcg
    71221 acatcgatat tcgcgacagt attcacagcg accgcattct gattgtgatg atccctgcac
    71281 tggacacatc ggctcatacc acgtcggcac tcggtcgtat gttcgtcacc cagcagagca
    71341 tgctcctcgc ccgtgacctg ggctaccggc tggaaggcac agatgcacag accctggaag
    71401 tcaaaaaata caaaggcagc ttcccctata tctgtatcct tgacgaagtc ggtgcctact
    71461 acacagaccg cattgctgtg gaggccaccc aggtacgttc actggagttt tccctgataa
    71521 tgacggcgca ggatcaggag cgaattgaag ggcagacctc cgccaccaac accgccacac
    71581 tgatgcagaa cgccggcacc aaattcgccg gacgcattgt cagtgacgac aaaacggcaa
    71641 gaacggtgaa aaatgccgcc ggtgaagagg cacgggccag aatgggcagc ctgcagcgcc
    71701 acgatggtgt gatgggtgag tcctgggtgg acggtaacca gatcaccatc cagatggaaa
    71761 gcaaaatcga cgtgcaggat ttgatcagac tgaatgctgg tgagttcttt accgttttcc
    71821 agggggatgt ggtgccgtca gcctctgtgt atattccgga cagcgagaaa agttgcgaca
    71881 gtgatccggt ggtcatcaac cgctatattt ccgttgaagc cccccggctg gagcgtctcc
    71941 gccgtctcgt ccccaggaca gtgcagcgca ggctccccac accggagcat gtcagcagca
    72001 tcatcggtgt actgacagca aaaccatccc gtaaacgtcg taaaaaccgt acagagcctt
    72061 accgtatcat tgatacattc cagcgccagt tggcaacagc gcagacatca ctggatctgc
    72121 ttccgcaata tgatacagat atcgaatccc gggcaaacga actgtggaaa aaggctgtgc
    72181 acactatcaa taacacaacc agggaagaac gccgggtatg ttatatcaca ctgaatcgtc
    72241 cggaagagga acactccggt cctgaagata taccttcagt gaaggcgatt ctgaatactc
    72301 tgctccccct ggagatgctg ttacctgtac cggatctaac tgcatccccg ccccataaaa
    72361 aaaatgtcgc tcagacacca tcaggtggaa aacaagagag tcgtaaaaaa agattctgat
    72421 aacacctgta tggctgattt taataacaac ctttacgcga ttattaatct cctccgctca
    72481 ccatagtaag cggaggattc aggatttcaa tccatcacca gaggtacaat aaatgtgtat
    72541 tagtagcccg gaaacaaact catggtctgt tatttatcgt aaaaattccg gagaagatat
    72601 caatatcact tccctgacgt ttaaaaatag tcttctggca gcccggacac tgatggtgcc
    72661 cgaaaactat atgatatgca tactcagaaa cggggagagg gtcagacgat gggacagaga
    72721 aatacttgca ggctcaaaca gatggtacaa gtgttctccc gataatttcg aaatactggg
    72781 taagctcccc ataataaata aagtcacaaa actgataaaa tcataatcta cggattttaa
    72841 tcagttaagc taaatcagga tgaacgcttt gattatgaat tatacatgaa aacaaatatg
    72901 ggcagcacaa aaaatggtgc cagggttccc cacgttcgta atagattcac ttctatggat
    72961 attggtatga attcccagat cccactctat tgtaaacaag acatttttat cttttatatt
    73021 caatggctta ttttcctgct aattggtaat accatgaaaa ataccatgct cagaaaaggc
    73081 ttaacaatat tttgaaaaat tgcctactga gcgctgccgc acagctccat aggccgcttt
    73141 cctggctttg cttccagatg tatgctattc tgctcctgca gctaatggat caccgcaaac
    73201 aggttactcg cctggggatt ccctttcgac ccgagcatcc gtatgagact catgctcgat
    73261 tattattatt atagaagccc ccatgaataa atcgctcatc attttcggca tcgtcaacat
    73321 aacctcggac agtttctccg atggaggccg gtatctggcg ccagacgcag ccattgcgca
    73381 ggcgcgtaag ctgatggccg agggggcaga tgtgatcgac ctcggtccgg catccagcaa
    73441 tcccgacgcc gcgcctgttt cgtccgacac agaaatcgcg cgtatcgcgc cggtgctgga
    73501 cgcgctcaag gcagatggca ttcccgtctc gctcgacagt tatcaacccg cgacgcaagc
    73561 ctatgccttg tcgcgtggtg tggcctatct caatgatatt cgcggttttc cagacgctgc
    73621 gttctatccg caattggcga aatcatctgc caaactcgtc gttatgcatt cggtgcaaga
    73681 cgggcaggca gatcggcgcg aggcacccgc tggcgacatc atggatcaca ttgcggcgtt
    73741 ctttgacgcg cgcatcgcgg cgctgacggg tgccggtatc aaacgcaacc gccttgtcct
    73801 tgatcccggc atggggtttt ttctgggggc tgctcccgaa acctcgctct cggtgctggc
    73861 gcggttcgat gaattgcggc tgcgcttcga tttgccggtg cttctgtctg tttcgcgcaa
    73921 atcctttctg cgcgcgctca caggccgtgg tccgggggat gtcggggccg cgacactcgc
    73981 tgcagagctt gccgccgccg caggtggagc tgacttcatc cgcacacacg agccgcgccc
    74041 cttgcgcgac gggctggcgg tattggcggc gctgaaagaa accgcaagaa ttcgttaact
    74101 gcacattcgg gatatttctc tatattcgcg gttcagcagg catgtcccct ttgagggcga
    74161 cccgacgaca ggataatcga ccttatggtg cgcaaatatt tcggcacaga cggtattcgt
    74221 ggcaaagcca acgaaggcgc gatgacggcg gaaaccgcct tgcgcgtcgg catggcggct
    74281 ggccgtgtct ttcgtcgcgg tgaccaccgc catcgtgtcg tgatcggcaa ggatacgcgc
    74341 ctgtcgggct atatgcttga acccgcgctc acagccggtt tcacctcgat gggcatggac
    74401 gtattccttt ttggcccgct gccgacaacg tataggaaga ataaacgccc ttttcaccca
    74461 agtccaacag ctttggaccg cagttgactc tttcgacacc cctgcgatgc aacccaatcc
    74521 ggctgacggg gagccagcaa cgctgaaaat ttaccctcct ctttcccact agcggctcct
    74581 tttccgacaa ccagcacggc ggatccctgc cgcggcgctg tgaacgcagc attttgattg
    74641 gtatcgttgg ccttcaggct cgtcagtcaa acagacccag gagcagctca gccggtggcg
    74701 cccggctttc gggtaacgcc ctggtcccgc tggtttcggc tttgtgcttc aggtgatcaa
    74761 ggatctgctt gatcact
//
LOCUS       DAZXUL010000002        70743 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZXUL010000002 DAZXUL010000000
VERSION     DAZXUL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70743
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.002"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..826
                     /locus_tag="ACH3L1_000079"
     CDS             <1..826
                     /locus_tag="ACH3L1_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFT4075013.1"
                     /translation="LSGDSGGQSSWLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGR
                     LSYSLEQSLDDDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGV
                     TLSQYLGNAFALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLP
                     DNVDLEQTTQFVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQ
                     SIVDEGGILYLSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            837..1928
                     /locus_tag="ACH3L1_000080"
     CDS             837..1928
                     /locus_tag="ACH3L1_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4075014.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1971..2831)
                     /gene="rsmI"
                     /locus_tag="ACH3L1_000081"
     CDS             complement(1971..2831)
                     /gene="rsmI"
                     /locus_tag="ACH3L1_000081"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFT4075015.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2896..4932
                     /gene="lpoA"
                     /locus_tag="ACH3L1_000082"
     CDS             2896..4932
                     /gene="lpoA"
                     /locus_tag="ACH3L1_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFT4075016.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4890..5285
                     /locus_tag="ACH3L1_000083"
     CDS             4890..5285
                     /locus_tag="ACH3L1_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFT4075017.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5305..5895
                     /gene="diaA"
                     /locus_tag="ACH3L1_000084"
     CDS             5305..5895
                     /gene="diaA"
                     /locus_tag="ACH3L1_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFT4075018.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5905..6480
                     /gene="dolP"
                     /locus_tag="ACH3L1_000085"
     CDS             5905..6480
                     /gene="dolP"
                     /locus_tag="ACH3L1_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFT4075019.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6594..7634)
                     /locus_tag="ACH3L1_000086"
     CDS             complement(6594..7634)
                     /locus_tag="ACH3L1_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFT4075020.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7707..8342)
                     /locus_tag="ACH3L1_000087"
     CDS             complement(7707..8342)
                     /locus_tag="ACH3L1_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFT4075021.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8470..8988
                     /gene="yhbO"
                     /locus_tag="ACH3L1_000088"
     CDS             8470..8988
                     /gene="yhbO"
                     /locus_tag="ACH3L1_000088"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFT4075022.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8968..9411)
                     /locus_tag="ACH3L1_000089"
     CDS             complement(8968..9411)
                     /locus_tag="ACH3L1_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFT4075023.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9462..9764
                     /gene="yhbQ"
                     /locus_tag="ACH3L1_000090"
     CDS             9462..9764
                     /gene="yhbQ"
                     /locus_tag="ACH3L1_000090"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFT4075024.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9751..10254)
                     /locus_tag="ACH3L1_000091"
     CDS             complement(9751..10254)
                     /locus_tag="ACH3L1_000091"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFT4075025.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10248..10772)
                     /locus_tag="ACH3L1_000092"
     CDS             complement(10248..10772)
                     /locus_tag="ACH3L1_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFT4075026.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10981..11976
                     /locus_tag="ACH3L1_000093"
     CDS             10981..11976
                     /locus_tag="ACH3L1_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFT4075027.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11985..12863
                     /locus_tag="ACH3L1_000094"
     CDS             11985..12863
                     /locus_tag="ACH3L1_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFT4075028.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12944..13951
                     /locus_tag="ACH3L1_000095"
     CDS             12944..13951
                     /locus_tag="ACH3L1_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFT4075029.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14069..15313)
                     /gene="mtr"
                     /locus_tag="ACH3L1_000096"
     CDS             complement(14069..15313)
                     /gene="mtr"
                     /locus_tag="ACH3L1_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFT4075030.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15467..17356)
                     /gene="deaD"
                     /locus_tag="ACH3L1_000097"
     CDS             complement(15467..17356)
                     /gene="deaD"
                     /locus_tag="ACH3L1_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFT4075031.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17349..17429)
                     /gene="yrbN"
                     /locus_tag="ACH3L1_000098"
     CDS             complement(17349..17429)
                     /gene="yrbN"
                     /locus_tag="ACH3L1_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFT4075032.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17536..18420)
                     /gene="nlpI"
                     /locus_tag="ACH3L1_000099"
     CDS             complement(17536..18420)
                     /gene="nlpI"
                     /locus_tag="ACH3L1_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFT4075033.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18529..20664)
                     /gene="pnp"
                     /locus_tag="ACH3L1_000100"
     CDS             complement(18529..20664)
                     /gene="pnp"
                     /locus_tag="ACH3L1_000100"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFT4075034.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20911..21180)
                     /gene="rpsO"
                     /locus_tag="ACH3L1_000101"
     CDS             complement(20911..21180)
                     /gene="rpsO"
                     /locus_tag="ACH3L1_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFT4075035.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21329..22273)
                     /gene="truB"
                     /locus_tag="ACH3L1_000102"
     CDS             complement(21329..22273)
                     /gene="truB"
                     /locus_tag="ACH3L1_000102"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFT4075036.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22273..22674)
                     /gene="rbfA"
                     /locus_tag="ACH3L1_000103"
     CDS             complement(22273..22674)
                     /gene="rbfA"
                     /locus_tag="ACH3L1_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFT4075037.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22838..25486)
                     /gene="infB"
                     /locus_tag="ACH3L1_000104"
     CDS             complement(22838..25486)
                     /gene="infB"
                     /locus_tag="ACH3L1_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFT4075038.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25511..26998)
                     /gene="nusA"
                     /locus_tag="ACH3L1_000105"
     CDS             complement(25511..26998)
                     /gene="nusA"
                     /locus_tag="ACH3L1_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFT4075039.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(27026..27478)
                     /gene="rimP"
                     /locus_tag="ACH3L1_000106"
     CDS             complement(27026..27478)
                     /gene="rimP"
                     /locus_tag="ACH3L1_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFT4075040.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27685..27761)
                     /locus_tag="ACH3L1_000107"
     tRNA            complement(27685..27761)
                     /locus_tag="ACH3L1_000107"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27725..27727),aa:Met,seq:cat)
     gene            28109..29452
                     /gene="argG"
                     /locus_tag="ACH3L1_000108"
     CDS             28109..29452
                     /gene="argG"
                     /locus_tag="ACH3L1_000108"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFT4075041.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSGDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29460..31086)
                     /locus_tag="ACH3L1_000109"
                     /pseudo
     CDS             complement(29460..31086)
                     /locus_tag="ACH3L1_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(31545..31631)
                     /locus_tag="ACH3L1_000110"
     tRNA            complement(31545..31631)
                     /locus_tag="ACH3L1_000110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31595..31597),aa:Leu,seq:gag)
     gene            complement(31646..31978)
                     /gene="secG"
                     /locus_tag="ACH3L1_000111"
     CDS             complement(31646..31978)
                     /gene="secG"
                     /locus_tag="ACH3L1_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFT4075042.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32206..33543)
                     /gene="glmM"
                     /locus_tag="ACH3L1_000112"
     CDS             complement(32206..33543)
                     /gene="glmM"
                     /locus_tag="ACH3L1_000112"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFT4075043.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33536..34384)
                     /gene="folP"
                     /locus_tag="ACH3L1_000113"
     CDS             complement(33536..34384)
                     /gene="folP"
                     /locus_tag="ACH3L1_000113"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFT4075044.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34474..36408)
                     /gene="ftsH"
                     /locus_tag="ACH3L1_000114"
     CDS             complement(34474..36408)
                     /gene="ftsH"
                     /locus_tag="ACH3L1_000114"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFT4075045.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36508..37137)
                     /gene="rlmE"
                     /locus_tag="ACH3L1_000115"
     CDS             complement(36508..37137)
                     /gene="rlmE"
                     /locus_tag="ACH3L1_000115"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFT4075046.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37263..37556
                     /gene="yhbY"
                     /locus_tag="ACH3L1_000116"
     CDS             37263..37556
                     /gene="yhbY"
                     /locus_tag="ACH3L1_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFT4075047.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37712..38188)
                     /gene="greA"
                     /locus_tag="ACH3L1_000117"
     CDS             complement(37712..38188)
                     /gene="greA"
                     /locus_tag="ACH3L1_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFT4075048.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38436..39869
                     /gene="dacB"
                     /locus_tag="ACH3L1_000118"
     CDS             38436..39869
                     /gene="dacB"
                     /locus_tag="ACH3L1_000118"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFT4075049.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            39933..40034
                     /locus_tag="ACH3L1_000119"
                     /pseudo
     CDS             39933..40034
                     /locus_tag="ACH3L1_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40055..41227)
                     /gene="cgtA"
                     /locus_tag="ACH3L1_000120"
     CDS             complement(40055..41227)
                     /gene="cgtA"
                     /locus_tag="ACH3L1_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFT4075050.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41243..42208)
                     /locus_tag="ACH3L1_000121"
     CDS             complement(41243..42208)
                     /locus_tag="ACH3L1_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFT4075051.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42335..42592)
                     /gene="rpmA"
                     /locus_tag="ACH3L1_000122"
     CDS             complement(42335..42592)
                     /gene="rpmA"
                     /locus_tag="ACH3L1_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFT4075052.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42613..42924)
                     /gene="rplU"
                     /locus_tag="ACH3L1_000123"
     CDS             complement(42613..42924)
                     /gene="rplU"
                     /locus_tag="ACH3L1_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFT4075053.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43182..44153
                     /gene="ispB"
                     /locus_tag="ACH3L1_000124"
     CDS             43182..44153
                     /gene="ispB"
                     /locus_tag="ACH3L1_000124"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFT4075054.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44380..44658
                     /gene="sfsB"
                     /locus_tag="ACH3L1_000125"
     CDS             44380..44658
                     /gene="sfsB"
                     /locus_tag="ACH3L1_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFT4075055.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44706..45965)
                     /gene="murA"
                     /locus_tag="ACH3L1_000126"
     CDS             complement(44706..45965)
                     /gene="murA"
                     /locus_tag="ACH3L1_000126"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFT4075056.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(46020..46274)
                     /gene="ibaG"
                     /locus_tag="ACH3L1_000127"
     CDS             complement(46020..46274)
                     /gene="ibaG"
                     /locus_tag="ACH3L1_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFT4075057.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46434..46727)
                     /gene="mlaB"
                     /locus_tag="ACH3L1_000128"
     CDS             complement(46434..46727)
                     /gene="mlaB"
                     /locus_tag="ACH3L1_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFT4075058.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46727..47362)
                     /gene="mlaC"
                     /locus_tag="ACH3L1_000129"
     CDS             complement(46727..47362)
                     /gene="mlaC"
                     /locus_tag="ACH3L1_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFT4075059.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47381..47932)
                     /gene="mlaD"
                     /locus_tag="ACH3L1_000130"
     CDS             complement(47381..47932)
                     /gene="mlaD"
                     /locus_tag="ACH3L1_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFT4075060.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47937..48719)
                     /gene="mlaE"
                     /locus_tag="ACH3L1_000131"
     CDS             complement(47937..48719)
                     /gene="mlaE"
                     /locus_tag="ACH3L1_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFT4075061.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48727..49536)
                     /gene="mlaF"
                     /locus_tag="ACH3L1_000132"
     CDS             complement(48727..49536)
                     /gene="mlaF"
                     /locus_tag="ACH3L1_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFT4075062.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49746..50711
                     /locus_tag="ACH3L1_000133"
     CDS             49746..50711
                     /locus_tag="ACH3L1_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFT4075063.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50725..51711
                     /gene="kdsD"
                     /locus_tag="ACH3L1_000134"
     CDS             50725..51711
                     /gene="kdsD"
                     /locus_tag="ACH3L1_000134"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFT4075064.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51732..52298
                     /gene="kdsC"
                     /locus_tag="ACH3L1_000135"
     CDS             51732..52298
                     /gene="kdsC"
                     /locus_tag="ACH3L1_000135"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFT4075065.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52295..52870
                     /gene="lptC"
                     /locus_tag="ACH3L1_000136"
     CDS             52295..52870
                     /gene="lptC"
                     /locus_tag="ACH3L1_000136"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFT4075066.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52839..53396
                     /gene="lptA"
                     /locus_tag="ACH3L1_000137"
     CDS             52839..53396
                     /gene="lptA"
                     /locus_tag="ACH3L1_000137"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFT4075067.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53403..54128
                     /gene="lptB"
                     /locus_tag="ACH3L1_000138"
     CDS             53403..54128
                     /gene="lptB"
                     /locus_tag="ACH3L1_000138"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFT4075068.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54176..55609
                     /gene="rpoN"
                     /locus_tag="ACH3L1_000139"
     CDS             54176..55609
                     /gene="rpoN"
                     /locus_tag="ACH3L1_000139"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFT4075069.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55632..55919
                     /gene="hpf"
                     /locus_tag="ACH3L1_000140"
     CDS             55632..55919
                     /gene="hpf"
                     /locus_tag="ACH3L1_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFT4075070.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56037..56528
                     /gene="ptsN"
                     /locus_tag="ACH3L1_000141"
     CDS             56037..56528
                     /gene="ptsN"
                     /locus_tag="ACH3L1_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFT4075071.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56574..57428
                     /gene="rapZ"
                     /locus_tag="ACH3L1_000142"
     CDS             56574..57428
                     /gene="rapZ"
                     /locus_tag="ACH3L1_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFT4075072.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57425..57697
                     /gene="npr"
                     /locus_tag="ACH3L1_000143"
     CDS             57425..57697
                     /gene="npr"
                     /locus_tag="ACH3L1_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFT4075073.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57910..58542
                     /gene="yrbL"
                     /locus_tag="ACH3L1_000144"
     CDS             57910..58542
                     /gene="yrbL"
                     /locus_tag="ACH3L1_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFT4075074.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58539..59266)
                     /gene="mtgA"
                     /locus_tag="ACH3L1_000145"
                     /pseudo
     CDS             complement(58539..59266)
                     /gene="mtgA"
                     /locus_tag="ACH3L1_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59263..59916)
                     /gene="elbB"
                     /locus_tag="ACH3L1_000146"
     CDS             complement(59263..59916)
                     /gene="elbB"
                     /locus_tag="ACH3L1_000146"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFT4075075.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60146..62482)
                     /gene="arcB"
                     /locus_tag="ACH3L1_000147"
     CDS             complement(60146..62482)
                     /gene="arcB"
                     /locus_tag="ACH3L1_000147"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFT4075076.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62578..63507)
                     /locus_tag="ACH3L1_000148"
     CDS             complement(62578..63507)
                     /locus_tag="ACH3L1_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFT4075077.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64182..68642
                     /gene="gltB"
                     /locus_tag="ACH3L1_000149"
     CDS             64182..68642
                     /gene="gltB"
                     /locus_tag="ACH3L1_000149"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFT4075078.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68655..70073
                     /gene="gltD"
                     /locus_tag="ACH3L1_000150"
     CDS             68655..70073
                     /gene="gltD"
                     /locus_tag="ACH3L1_000150"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFT4075079.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70257..70661
                     /locus_tag="ACH3L1_000151"
                     /pseudo
     CDS             70257..70661
                     /locus_tag="ACH3L1_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
BASE COUNT        17318 a        19182 c        17572 g        16671 t
ORIGIN      
        1 gttgtctgga gattcagggg gccagtctag ctggttaccg cgtagcctgg tttcctatca
       61 gatgaccagc cagaaagatc gcccaaccca acatgaaatg cgtcttgatg gctcactgct
      121 ggatgatggt cgcctgagct atagcctgga acaaagtctg gatgacgata acaaccataa
      181 cagtagcctg aacgccagtt accgttcacc ttatggaacc ttcagtgccg gatacagtta
      241 cggtaatgac agcagccaat acaattacgg cgttaccggc ggcgtggtta tccatcctca
      301 tggcgtgacg ctctcgcaat atctgggcaa cgcttttgcg cttatcgatg ctaacggggc
      361 ttctggcgtg aggatacaaa actatccggg gattgctact gatccctttg gctatgcagt
      421 ggttccttat ctcacgactt atcaggaaaa ccgtctctcg gtagatacta cgcagctgcc
      481 cgataacgtc gatcttgaac aaacaacaca gtttgtggtg cccaacagag gtgcaatggt
      541 agcggcgcgt ttcaacgcca atatcggtta tcgcgtactt gttacagtca gcgatcgcaa
      601 cggtaaaccg ttgccctttg gcgctcttgc cagcaacgat gatacggggc aacaaagtat
      661 cgtcgatgag ggcggcatac tatatctctc tgggatatcg agtaaatcac aaagctggac
      721 tgtacgctgg ggaaatcagg cagatcaaca atgtcagttt gcttttagta caccggattc
      781 agaaccaaca acctctgtat tacaaggcac agcgcagtgc cattaaggat aaaaaaatga
      841 aaagagcgcc tcttataaca ggacttttgt tgatatccac atcctgcgct tatgcctcct
      901 caggagggtg tggagccgac agcactagcg gtgcgacaaa ttacagcagt gtggttgatg
      961 atgttacggt gaaccagaca gataacgtga caggacggga gtttacctct gcaacgctaa
     1021 gtagcactaa ctggcaatac gcctgttcct gctctgcggg taaggcagtt aaacttgtct
     1081 atatggtcag ccccgtactt accaccactg gacatcagac aggatattac aaactcaatg
     1141 acagcctgga tattaaaacc acattacagg caaacgacat tccaggactc acaaccgacc
     1201 aggttgtctc tgttaacacc cgattcacac agataaaaag cagcaccgta tattctgctg
     1261 caacccaaac gggtgtttgc cagggtgaca cgtctcgtta tggacccgtt aatattggtg
     1321 caaataccac ctttaccctg tatgtcacca agccatttct cggctcgatg accattccga
     1381 aaacggatat tgccgtcatt aaaggcgcgt gggtcgatgg aatgggaagc ccgtctacag
     1441 gtgacttcca tgatttagtc aagttatcga ttcagggaaa tctcaccgcc ccacagtcgt
     1501 gcaaaattaa tcagggcgat gttattaaag ttaattttgg attcatcaat ggtcagaagt
     1561 ttaccacccg caatgccatg ccagacggtt ttactccagt agactttgat atcacttatg
     1621 actgtggtga tacttcaaag attaaaaact cgttgcaaat gcgcatcgac ggtacaactg
     1681 gggtagtaga ccagtacaac ctggtcgcca ggcgaagaag ttcagacaat gtgcccgatg
     1741 tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta
     1801 tccttcccgt tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc
     1861 tggtcggtgg tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatgg
     1921 tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc
     1981 tgctccagcg catacttata cagcgcattt ttcttcacgc catgaatttc tgcggccagc
     2041 gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg
     2101 gcgggtaagt cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg
     2161 cgacggtttt catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg
     2221 gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc
     2281 gcaacgatat cttccaggct atctaacaga cggtgggtag attcataaaa aatcagcgtg
     2341 cgcggctccg cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa
     2401 aagccttcgt aacagaaacg gtcagatggt aaacccgcgg cgcttaacgc agtgatagca
     2461 gcacacggtc cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga
     2521 tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc
     2581 ccctcttgca gctttgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc
     2641 gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aaccggtgtg acgagtatcc
     2701 tcggcggcaa tcagatcaac ggcctgtaat acctctaacg tacgctgggt gatatccgcc
     2761 agattgccga ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg
     2821 tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat
     2881 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     2941 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     3001 actgcttata tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag
     3061 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     3121 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     3181 tctcagcgtc gcgagaaaac actgctggcg gcagagatta aactggcgca gaaagatttt
     3241 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     3301 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     3361 cgcgcgttaa ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat
     3421 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     3481 gccgacgaaa atattctgca aggctggctg gatctgcagc gcgtctggtt tgataaccgt
     3541 aacgatcccg acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat
     3601 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     3661 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     3721 attcagcaag gctttgaagc cgcgaaaaat atcggcactc agccagtggc ggctcaggta
     3781 gctgccgcac ctgccgcaga cgtagcagaa caacctcagc cgcaaactgc ggatagcgtt
     3841 gccagcccgg cacaagcctc ggttagcgat ctgaccggtg atcagcctgc ggcccagccg
     3901 gtgcctgtaa gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc
     3961 gcagagctga aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt
     4021 cagcaggatg gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg
     4081 ctgaagagca acaccccgct gaacgtactg gcactgaacc agccggagaa tatcgaaaac
     4141 cgcgtcaata tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt
     4201 catattcgtg accagggtaa acaagcgccg ctggtgctga tcccacgcag tgcattgggc
     4261 gatcgcgtag ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg caccgttctg
     4321 caacaaaaat ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt
     4381 gctttaacgg gtagcccgat tactcccaga gcgacaaccg actccggcat gacgaccaac
     4441 aatccaacgc tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc
     4501 gatgcggtct acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc
     4561 atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca gttcccgcag cgcacaaggc
     4621 accgcaggcc cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg
     4681 ctggcaggcg gtaatctgcc gttaatgcag caggcgctca gcgcggtgaa caacgattat
     4741 tcgctggctc gcatgtatgc gatgggcgtt gatgcctggt cgttggcaaa tcatttctca
     4801 caaatgcgcc aggttcaggg ttttgaaatc aacggtaata ccggaagtct gacggctaac
     4861 ccggattgcg tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc
     4921 cccgccagtt aaccaccaaa cagaccggcg atgcgtggga agcacaagcg cgtcgctggc
     4981 tggaaggcaa aggactgcgg tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg
     5041 atctgataat gcgtgaaggc ctgaccaccg tttttgtcga ggtacgctat cgccgctctg
     5101 cgctttatgg cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga
     5161 ctgcccgctt gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg
     5221 atgtggtagc cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact
     5281 cataattaag gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc
     5341 attcaaactc aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg
     5401 acgctggttc agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc
     5461 gctgccaatg cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc
     5521 agcttacctg ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat
     5581 cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta
     5641 ttgttagcca tttccacccg tggcaacagc cgcgatattg ttaaagcagt tgaagccgcc
     5701 gttacgcgtg atatgaccat tgtggcattg accggctatg acggcggcga acttgcaggt
     5761 ttgttagggc cacaggatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag
     5821 gaaatgcata tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct
     5881 caccaggatg attaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc
     5941 gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc
     6001 aaagccgcaa ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg
     6061 cgcgtgaaca gcgcattgtc gaaagacgaa cagattaaga aagaaacccg tattaatgtc
     6121 acggcctatc agggcaaagt gctgctggtt gggcagtcac caaatgctga actttcggct
     6181 cgcgccaaac agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt
     6241 cagggccagc cgattggtct gggcgaagca tctaacgata cgtggatcac caccaaagtg
     6301 cgttcgcagc tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa
     6361 aacggtgaag tgttcctgat ggggctggtg actgaacgtg aagcgaaagc ggcggcagat
     6421 attgccagcc gggtgagcgg cgtaaagcgg gttaccacag cgtttacgtt tattaaatag
     6481 cagccccttg taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgatgca
     6541 aaccgtaggt cggataaggc gtttacgccg catccgacat gttttccctc aaatcagaac
     6601 aacagcgcca gcccgccgac aatcacacca gacactgcca ccatcgcccc tgtcagccat
     6661 aaggctttcg ccgggaacgc tttgcgcagc ataatcagtg acggcaaact cactgccggg
     6721 agcgtcatca acaatgccag cgccggagcg gttcccatac ctgccagcat catcgtttgt
     6781 acaatcggaa tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc
     6841 accacccaca tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcgt
     6901 gcggcaccca acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagtgtc
     6961 catagcgccc tgccccagcg gctaaaaaaa ccgctctgtg cttccggtat gtcaatttcg
     7021 accggtgcct gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc
     7081 aacaccatca ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaac
     7141 cccataaaca ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca
     7201 ccgcccatcg atacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta
     7261 cacatcatgc ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc
     7321 ccaagcgtac gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc
     7381 acccccagca ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc
     7441 gcttgccacg gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg
     7501 gctgcagtaa acgctttgcc gtagtaaggt tgccatttca cataccagag accggcaatg
     7561 acaacgagaa agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa
     7621 gactgaccag tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta
     7681 cgctcactat caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt
     7741 aaaatcgtca cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg
     7801 tctctcgcat caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc
     7861 gtttcgttca tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc
     7921 aatttcggcc agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag
     7981 aaaaacggcg agtgggcatt ggcacccata gcactgacca ccagcatatg ctgcgcgccc
     8041 agtcgccgcc cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc
     8101 tctttgctcc ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatgggt
     8161 tcggtgacct gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc
     8221 atatcgccca acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc
     8281 aacatccgca gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc
     8341 atcttcgttc ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca ccagtcttaa
     8401 tagaccatga ggtaattatc cccgattgtg gaaaattcgc ctcatccaat gcaacaacgc
     8461 ggaggaagca tgagtaagaa aattgccgtt ttaatcactg atgaatttga agattcagaa
     8521 tttacttcac ccgcagacga gttccgtaaa gccggacacg aagtgattac tattgaaaaa
     8581 caagcgggta aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc
     8641 atcgatgaag tgacgccagc ggagtttgat gccctgttgc taccgggtgg tcattcaccg
     8701 gattatctgc gtggcgacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc
     8761 aaacctgttt tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg
     8821 gggcgcaaac tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgtggaa
     8881 ttttacgatc aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac
     8941 gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca
     9001 aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat
     9061 ttcccacacc ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg
     9121 cgcgaggtcg ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg
     9181 aattaacgct accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc
     9241 actcatctgc gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc
     9301 atcaaaaaga taaaaggcat tagcgcacca gagttcccct tcctgctgca cacaccaggt
     9361 gacaacatgt tgttttgcca gccaacggct gatggcgatg agtgtttcca ttgctgttct
     9421 cttttatact gtgggcctga ccttaacatg cgatgcagac gatgacaccc tggtttcttt
     9481 acttgatccg taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc
     9541 gctatcagca gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga
     9601 cgctggcgtt ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg
     9661 ttaagcaact gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag
     9721 aactgttgag cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc
     9781 aaccagaccg gttacgccat tcagcgcgtc atccgctaaa cgatgtacca ggaaggcgct
     9841 ttcagtgccc ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg
     9901 actgtacagc gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga
     9961 atcgagtcct tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac
    10021 cgccaacggt gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa
    10081 tgccacatag ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa
    10141 gccatcttca cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg
    10201 cagcagggca tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca
    10261 cctaccgatg tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc
    10321 agttgcagca gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc
    10381 aggtttttca catacacccc cagctccgta tcgccttcaa tcaccagccg acgctggaag
    10441 aagagcgtat ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca
    10501 ctaaaactca catcagcttg cgtgttctgg ctgaccacca gtttgccatt caccaccgag
    10561 gtaaaccatt gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac
    10621 tccagctcgc catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc
    10681 tggcgtttta gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa
    10741 tgcacaatac gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat
    10801 cctattttgc catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat
    10861 tgctgctacc accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta
    10921 actaaaatcc cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt
    10981 atggagctgc tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc
    11041 gcagatgctg tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt
    11101 aactttaccg agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa
    11161 cttcacatcg cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc
    11221 gtggatatgg cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg
    11281 gagtacgccg ccgagcgtta cccgcatatt gaacgtcatg tgtcagtgca ggcttcggcg
    11341 accaatgaag aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg
    11401 ccgcgcgtgt tgtcgattca tcaggtgaaa cagctggcac gggttactcc tgtcccgctg
    11461 gaagtgtttg ctttcggcag cctgtgtatt atgtcggaag gccgttgcta tctgtcgtcg
    11521 tatctgacgg gtgagtcgcc caacactgtg ggtgcgtgtt ctccggcccg tttcgtgcgc
    11581 tggcaacaaa cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat
    11641 caggacggcg aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc
    11701 gagcgctatc acgcgctgga agaaccaacc agtctcaata ccctggaact gctgccggag
    11761 ttaatggctg cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat
    11821 gtcagccagg cggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa
    11881 aacttcgtac cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcaa
    11941 accacccttg gcgcgtatca ccgtaaatgg cagtgagaag agcaatgaaa tattccttag
    12001 ggccagtgct gtggtactgg ccaaaagaga cgctggaaga tttttatcag caagccgccg
    12061 ccagcagcgc cgacgtgatt tatcttggtg aagcggtatg cagcaagcgc cgggcaacca
    12121 aagttggcga ctgtctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtgc
    12181 tctccacgct ggcgctggtg caggcatcct ctgaactggg cgaactgaaa cgctatgttg
    12241 agaacggtga atttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac
    12301 gcaaactgcc gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa
    12361 tattgctcaa acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc
    12421 tggtgaatct gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag
    12481 tcctgagcta cggtcatcta ccgctggcct actccgcccg ctgctttacc gcgcgttcgg
    12541 aagaccgccc gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg
    12601 tgctgtcgca ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct
    12661 acgtttacaa cctcggtaac gagctggcat ccatgcaggg attggttgat gtagtccgcc
    12721 tgtcaccgca gggcactgac actttcgcga tgctcgacgc cttccgcgct aatgaaaatg
    12781 gcgctgtgcc actgccgttg acggcaaaca gcgactgtaa cggctactgg cggcggctgg
    12841 caggactgga gctgcaagcc taagtaaata gctcactttg ttaacaactt taactattct
    12901 ttaatgcagt attaaagatt aatcggtaac aaagtgagct gttatgactg ataaaaccat
    12961 tgcgttttcg ctactcgatc tggcccccat acccgaaggc tcttcagcgc gggaagcgtt
    13021 ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc ggctatcatc gctactggct
    13081 ggcagaacac cacaatatga ccggcattgc cagtgctgca acatcggtat tgattggcta
    13141 tctggcggcg aataccacca cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca
    13201 cttaccgttg gtcattgccg aacagttcgg cacactcaat acgctctatc cggggcgaat
    13261 cgatttgggg ctgggtcgtg ctccgggtag tgaccaacgg acaatgatgg cgctacgtcg
    13321 tcatatgagc ggcgatattg ataatttccc ccgcgatgtc gcggagctgg tggactggtt
    13381 tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca ggctatggcg agaaaatccc
    13441 cgtgtggttg ttaggctcca gcctttacag tgcacaactg gcggcgcagc ttggtctgcc
    13501 atttgcgttt gcctcacact tcgcgccgga tatgttgttc caggcgctgc atctttatcg
    13561 cagtaacttc aaaccgtcgg ctcggctgga aaaaccatac gcgatggtgt gcatcaatat
    13621 tatcgccgcc gacagcaacc gcgacgctga atttctgttt acctcaatgc agcaagcctt
    13681 tgtgaagctg cgccgtggcg aaaccgggca actgccgccg ccgattcaaa atatggatca
    13741 gttctggtca ccgtctgagc agtatgacgt gcagcaggcg ctgagtatgt cgttggtagg
    13801 cgataaagcg aaagtgcgtc atggtttgca gtcgatcctg cgcgaaaccg acgctgatga
    13861 gattatggtt aacgggcaga ttttcgacca ccaggcgcgc ctgcattcgt ttgagctggc
    13921 gatggatgtt aaggaagagt tgttgggata gtgtgtctta acgcgggaag ccttatccga
    13981 gctggcaacg ctgtcctacg taggcctgat aagcgaagcg catcaggcat tgtgtaggca
    14041 gcagaaatgt cggataaggc accgctgatt actgatacac cggcagtaaa ttaaagctcg
    14101 ataaaatatg caccagtgcg ttgccgacgc caaacaccag aatcagcgca atcatcggct
    14161 tgccacccca gacgcggaat ttcgggctgc caaagcgttt acgcgatgcg cgggccagca
    14221 gtgccggaac aattgccgcc cagatggtag ccgctaaacc agcataacca atggcataca
    14281 ggaatccgtt cgggaacagc agaccaccca caacaggcgg ggcaaaggtc agcaatgccg
    14341 ttttcaagcg gcccacagcc gagtcgtcga aaccaaacag atctgccaga tagtcaaaca
    14401 aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac acgaccagca
    14461 gaaggtccag gctacggctg ttcagcacgc cgcttaatgc ttgtaccaac acatcaatat
    14521 taccgccctt ctcggcgatg ccgataaact ccggacgagg gatgttgccc attgtcgcca
    14581 gcaaccagat ggtatacagc gccagtgcca tcagcgtacc gtacaccagg catttcacga
    14641 tggttttcgg atctttgccg taatacttca tcaggcttgg cacgttaccg tgataaccaa
    14701 acgacgccag gcagaacggc agggtcatca gcaaatacgg cgcataagat gcattgcttt
    14761 cggcaacgtt gaacaatgtc gcaggctgca catgccccag caggctaccg aaggtgagga
    14821 agaaggtaat gactttcgcc cccagcacaa tcgccgtcat gcggctgacg gctttagtac
    14881 tcaaccacac cacaaacgct accagcagtg caaaaccaaa acccgccgcc cgtgccggga
    14941 cgtttagcga catctctgcg aaggtgtgat gcagaatcga accactggca gaaatatagg
    15001 cataggtcag gatatagagc acaaaggcaa tggaaatgcc gttgaccacg ttccagcctt
    15061 tgcccagcaa atctttggtg atggtgtcaa aactcgaacc gattcggtaa ttcaggttag
    15121 cttccagaat catcaagccg gaatgcagca tacagaacca ggtaaagatc agcgctgcca
    15181 ttgaccagaa aaaccacgcc ccggacatga ccactggcag agaaaacatc cctgcgccaa
    15241 taatggtgcc gccgataatc accacgccgc caagcagcga cggtgacgtt tgggtggtgg
    15301 ttagtgttgc catgagggct tctctccagt gaaaaatagt gcgactgcgt tgttatgcat
    15361 tgcactgtac cagtacatga gtacaaaaga cagaaaaaaa gccccgatgg ttaaaatcgg
    15421 ggctgtatat attattttac agattgtgtt cgctgttcag cgatgattac gcatcaccac
    15481 cgaaacgacg acgaccggta gagtcatcac gacgcggtgc gcggccttca cgacgttcgc
    15541 cgctaaaacg acgaccatca ccacgaccac cttcacggcg ttcaccgctg aagttacgac
    15601 cgccttcacg acgctcgcca ccgaaaccac gaccaccgcc acgacgctca ccgccagtat
    15661 gcggctgtgc atcgcccagt aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg
    15721 taaagtgttg cagcacttca cccggcatac ctttcggcag ttcgatggtg gagtgagaag
    15781 caaacagctt gatgttaccg atgtaacggc tgctgatgtc gccttcgtta gcaatcgcgc
    15841 caacgatatg acgaacttca acaccatcat cgcggcccac ttcaatgcgg tacagctgca
    15901 tatcgccaac atcacgacgt tcacgacgcg gacgatcttc acggtcgcca cgcgggccac
    15961 ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg gaattcacgt ttcggacgca
    16021 tcggcgcatc tggcggaaca atcagagtac gttcaccctg tgccattttc agcagtgccg
    16081 cagccagagt ttcgagatcc agctcttcac cttctgcagt cggctgaatt ttgctcagca
    16141 gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt
    16201 tttccagacg gcgtttgccc agcagttctg cgttcggcag ttctacttcc ggaatagtca
    16261 gcttcatagt acgttcaatg ttgcgcagca gacgacgctc gcggttctca acgaacagca
    16321 gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat acggtgaacg taagactcag
    16381 aatccatcgg gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc aggccacggg
    16441 ccgcaacgtc ggtcgcaatc aggatgtcca gacgaccgtc tttaaggcgt tccagtgtct
    16501 gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc gctattgtag ccgttacgct
    16561 caagagcttc agccacttcc agagtcgcgt ttttggtacg aacgaagata atcgccgcat
    16621 caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt tttacgcata ccccacacag
    16681 tccagtagct ctggctgatg tcaggacggg tagtcacgct ggactgaata cgcacttcct
    16741 gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga
    16801 acagagcggt ctgatgacct tccgggatct gcgccataat ggtttcaacg tcttcgatga
    16861 agcccatgcg cagcatttcg tcagcttcat ccagaaccag accgctcagt ttagagaggt
    16921 ccagagtgcc acgtttcagg tggtccagca gacggcccgg agtaccgaca acgatctgcg
    16981 gcccctgacg cagggcgcgt aattgcacgt cataacgctg gccgccgtac agggcaacca
    17041 catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca
    17101 gttcgcgggt cggtgccagc accagaatct gtggtgcttt cagctcagga tcaagattct
    17161 gcagcagagg taaagagaat gctgcagttt ttccgctccc cgtctgggcc atacccagaa
    17221 cgtcgcggcc attcagcaga tgtggaatac actctgcctg aattggagat ggtttttcgt
    17281 aacccagatc gttaagggct tcaaggatag gagccttcag gcccagatct gcaaaagtgg
    17341 tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa tggcggccag tctacataac
    17401 tcatcatgaa attgatcagc aattttcatt gaaaagtgtg aaccggctca aagtaggtgt
    17461 attaacgaac aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa gattacgggc
    17521 tgatgtgtac gtcagctatt gctggtccga ttctgccagg tcatcttggt cctggcccag
    17581 gagcgataat tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac
    17641 cgccagtttg aacagtgccg tggcgctgtc caaatccccc agacttaggt agtacttacc
    17701 taaatagaag ttggtttcac tgagatgctc agcgagcgag gtgttatccg ttgcgtccgc
    17761 cttgagcctt tccattaacg tttgttcgct aatgttgccc aggtagaact cgacaatgtt
    17821 ccatccccac tgttccttat ccgatttttc gaagtgctgt ttcaatactt ctttagcctg
    17881 cttctcatcg agcttctgct cggcgagata aagccacaga ctacggaaag gatcattggg
    17941 atcgtcttga taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata
    18001 taatgcaatc ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct caagtacaga
    18061 atcaaacgct tcataggcag catcaaaatt gcctgcctgc gttaaatata tgcctaagta
    18121 attgaatact tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc
    18181 taatgccctc agaccgagac tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg
    18241 ttcgtcatcg gttaaagccc gactggcaag gatttgttcc atacgtgcca gaatcacttc
    18301 ctgctgtaaa gtcggttgca atggtaccgc gaggacttca cttttacgcc aggaagtatt
    18361 actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat
    18421 ttcccactcc cgaagaccac ggttgaatga acgtcctgtt cccggttgct aacaaggcgt
    18481 cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt actcgccctg
    18541 ttcagcagcc ggagcttccg gtgctgcagc aggttgagac tgctcagtcg cttctttaat
    18601 gctcagacgg atacggccct ggcgatcaac ttccagaact ttcaccggta cttcctgacc
    18661 catctgcagg taatcggtca ctttctcaac gcgtttgtca gcgatttgag agatgtgtac
    18721 cagaccttct ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga tacgggtcac
    18781 tttaccagtg tagacgcggc ccacttcgat ttctgcagtg atctcttcga tacgacgaat
    18841 agcatgtttc gctttctcgc cgtcggtcgc tgcaatcttc acagtaccgt catcttcgat
    18901 ttcgatggtg gtgccagttt cttcggtcag ggcacggata acagagccgc ctttaccgat
    18961 aacgtctttg atcttgtccg ggttgatctt gatggtatgg atacgtggtg cgaactcaga
    19021 gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg cccaggatat gcagacgcgc
    19081 acctttagcc tggttcagcg caacctgcat gatctctttg gtgatacctt caattttgat
    19141 atccatctgc aatgcagaga taccctcgcg ggaacccgca actttgaagt ccatatcgcc
    19201 caggtgatct tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc cttctttcac
    19261 cagacccatt gcgatacccg caacggcagc tttgatcggc acacctgcgt ccatcagcgc
    19321 cagagacgcg ccgcacacgg aagccataga agaggaaccg ttggattcag tgatttcaga
    19381 cacaacacgt acggtgtacg ggaatttgtc catatccggc atgactgcca gcacgccgcg
    19441 cttcgccaga cgaccgtgac caatttcacg acgcttcgga gaaccgacca tgccggtttc
    19501 gcctacggag tacggaggga agttgtagtg gaacaggaag gtatcggtac gttcgcccat
    19561 cagttcatca agaacctgcg cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg
    19621 cgtttcacca cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac
    19681 atccagacca cggatcatat ctttttcacg accgtcgata cgcggttcgc ctgccagtac
    19741 gcggctacga acaacgtttt tctcgatcgc gtgcagaatt tcacccagtt cgttttcgtc
    19801 cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca gatttgatga catcaacctg
    19861 cgcataacgc tcttgtttgt cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag
    19921 tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg
    19981 tttaccggct tctttcacca gttcattgat gttctgaata acaacctgct gttgttcatg
    20041 accgaacact actgcgccca gcatctggtc ttcgctcagc agttcagctt cagattcaac
    20101 catcagtaca gcggcttcag taccggcaac aaccagatcc agtttgctct ctttcaactc
    20161 gtcctgagtc gggttcagta cgtactggtc attgatgtaa cctacgcgag cagcaccaat
    20221 cggaccattg aacggaatac cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac
    20281 gatatccggg ttaacttgcg ggttaacaga aaccacggtg gcgataacct gaacttcgtt
    20341 gacgaagcct tccgggaaca gcggccgaat cgggcggtca atcagacgcg cgatcagggt
    20401 ttcgccttcg cttgggcggc cttcacgacg gaagaagcta cccgggatac gaccagcagc
    20461 gtaggtacgc tcctgatagt taacggtcag tgggaagaag tcctgacctg gtttggcttt
    20521 tttctggcca acaacggtaa caaataccgc ggtgtcatcc atgctaacca taacagcggc
    20581 agtagcctga cgagccatca tgccggtttc cagagtcacg gtgtgttggc cgtactggaa
    20641 tttacgaacg atcggattaa gcaatgtaat atcctttctc tttcttagac agtaccttac
    20701 ggcactggtg ttaatacccg atcttctgcg catcctcgcg actaatgaca accctaaccc
    20761 agctctatgt gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg aagatcattc
    20821 atagcaacaa tacattagtt tccagtgaat tgctgccgtc agcttgaaaa aaggggccac
    20881 tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag
    20941 ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg agcagtttac gacgctgaga
    21001 aaccatgcgc agcagaccac gacggctgtg gtgatctttt ttgtgctctg caaagtggcc
    21061 ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga acttcagtag aaccggtgtc
    21121 gtttgcgtca cgaccaaact cagaaacgat tttagctgtt gcttcagtac ttagagacat
    21181 tttaaaactc caaagtatat agaatgaaag gacgccgatc tctaattcag cgatcccagt
    21241 gtacgttacg caaagtgtta aacaatttac gcgacgttaa gcggcagtat tctactcgta
    21301 gcgacctgtt atcgcaagac ggttaacatt acgccgggta ttcaaccacc aggcgacgag
    21361 gcgcaacgcg gccttcatcg tcaatttcgc ccataccgat aaatttgccg ttctcacctt
    21421 ctgtaacgcg aaccagtcct tccagcggcg caccggatgt acgaactggg ttaccatttt
    21481 tgaagtaaac agaagacgtt aacggaagat tcaccaccgg gtagtccgaa gctggactgt
    21541 ccattggcat cagtaatgga tcaagtaact ccgcagccgg aatatcctgc tgttcggctt
    21601 gttcgaccag ttcacgcaga tgttccaggg tcacaatccg ttcaaccgga tatttactta
    21661 ccgccagacg acgcaggtaa ataacatgcg cgccacaacc gagtttttca cccaggtcat
    21721 caatgatggt gcggatataa gttccttttg agcagtgaat ttccagctcc agctcattgc
    21781 cttcgtggcg aataaacagc aattcataaa cggtaatcgg gcgcgcttca cgcggtactt
    21841 caatgccctg acgcgcatat tcgtacagtt ttttaccctg atatttcagt gctgaataca
    21901 tcgaagggat ctgttcgata tcgccacgga aagtatccag cgccgccgcc agttgctctg
    21961 cgctaaaggt taccggacgt tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac
    22021 gctgtccaag acgcgcaatg acccgatagc gtttgtcgga gtccagcaga tactgggaaa
    22081 acttcgtcgc ttccccgagg caaattggca acatgccagt tgccagcggg tccagcgcac
    22141 cggtatgccc ggcacggttg gcgttataaa tccgtttcac tttttgcagc gcatcattgc
    22201 tggacatacc ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac
    22261 gaggacgact cattagtcct ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg
    22321 tttgaccacg ctggtcacca ggtttgacat gcgcatccct tcaaccagag agttgtcgta
    22381 gaagaaggtc agttccggca cgatacgcag gcgcatcgct ttccccagca ggctgcggat
    22441 gaaaccagaa gcttcttgca acgctttgat gcccgcttta accgcgtctt catctttgtc
    22501 gttgaggaac gtcacatata ctttggcata cgccaggtcg cgagacattt cgacaccgga
    22561 aacggtggtc atcatgccca ggcgaggatc tttaatttca cgctgcagga tgagagcgat
    22621 ctctttttgc atttcctgcg ctacgcgctg cgggcgacca aattctttcg ccataataaa
    22681 ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag gtggaagggc
    22741 tgttcacgtt ggcctgataa gacgcgccag cgtcacatca ggcaatccat gccggatgcg
    22801 gcgtaaacgc cttatcccgc atggaaccct aaaaacctta agcgatggta cgttggatct
    22861 cgatgatttc gaatacttcg atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc
    22921 cgataccaca ttccataccg ttacggactt cgttaacgtc atctttgaag cggcgcaggg
    22981 actccagctc gccttcgtag ataaccacgt tgtcacgcag aacgcggatc gggttgtgac
    23041 gtttaaccac accttcggta accatacagc ctgcgatggc accaaatttc ggcgatttga
    23101 acacgtcacg aacttccgcc agaccgataa tctgctgttt cagttccgga gacagcatac
    23161 cgctcatcgc cgctttcact tcgtcaatca ggttatagat gacggagtag taacgcagat
    23221 ccaggctttc cgcttcaatc actttacgtg cagaggcatc agcacgtacg ttgaagccaa
    23281 ccaggatggc gttggacgcc gcagccaggg tggcgtcggt ttcggtgata ccacctacgc
    23341 cagaaccgat gatcttcact ttaacttcgt cagtagacag tttcagcaag gagtcggaga
    23401 tcgcttccac agaaccctgt acgtctgcct tcaggacgat gttcacttcg tgaacttcgc
    23461 cttcggtcat gttggcgaac atgttctcga gtttagattt ctgctgacgc gccagtttaa
    23521 cttcgcggaa tttaccctga cgatacagtg caacttcacg cgctttcttc tcgtcacgta
    23581 caacggtaac ttcatcaccc gcagccggta cgccggacag gccgaggatt tccaccggaa
    23641 tggacggacc cgcttccagc acttcctgac ccagttcgtt acgcatcgca cgaacacgac
    23701 cgtattcgaa gccacacaga acgatatcgc ccttgtgcag agtaccttca cgtaccagaa
    23761 cggtagcaac cggaccacga cctttatcga ggaaggattc gataaccgca ccgctcgcca
    23821 tacctttacg taccgctttc agctccagaa cttccgcctg cagcaggata gcgtccagca
    23881 gttcatcgat accagtaccc gctttcgcag acacatgtac gaactggctt tcaccgcccc
    23941 actcttccgg caggatgccg tactgggaga gttcgttctt aacgcgatcc ggatcggctt
    24001 ctggtttatc gatcttgttc actgcaacga ccaccggaac ctgcgccgct ttcgcgtgct
    24061 ggattgcttc gatcgtctgc ggcattacgc cgtcgtcggc agcaacaacc aggactacga
    24121 tgtccgttgc ctgcgcacca cgagcacgca ttgaagtaaa cgcggcgtgc cccggggtgt
    24181 ccaggaaggt gatcatgccg ttttcagttt caacgtggta tgcaccaatg tgctgggtaa
    24241 tgccgcccgc ttcgccagag gccactttcg ttgaacgaat gtagtccagc agagaggttt
    24301 taccgtggtc aacgtgaccc atgatggtca caaccggcgc gcgcggttca gccgcagcac
    24361 ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc aggataactt
    24421 tatggcccat ctcttcagca accagctgtg cggtttcctg atcgataacc tggttgatgg
    24481 ttgccattgc gcccagtttc atcatcgctt tgatgacctg agagccttta accgccatct
    24541 tgttcgccag ttcgccaacg gtgatagttt cgccgatcac aacgtcacgg ttaacggcct
    24601 gagcaggctt ctggaagcct tgctgcagcg aagaaccttt acgttttccg cctttaccgc
    24661 cacgtactgc tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt
    24721 tcttcggacg cgctgctttc gcgttacgac cacggccacg gccgccttcg acttcacgat
    24781 cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt gacgtgataa tcgctggaat
    24841 cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc cgccatacga cgtgcttctt
    24901 cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc ttcttcttca gctttacgct
    24961 tgagctctgc agcttcctgc tcacggcggg ctttttcagc ctgggcgttt ttagtcatat
    25021 cgtcttgttg attgctcact ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag
    25081 cttcacgctt agcttgttct gcggcctcac gttcagcttt ttgttgcgcc tcgcgtttag
    25141 ccgattcttc tgcctcacga cgggcttgct cttccgctgc aaggcgttca gcctcttgcg
    25201 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    25261 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    25321 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    25381 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    25441 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    25501 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    25561 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    25621 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    25681 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    25741 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    25801 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    25861 tcgagaaacc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    25921 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    25981 tcattacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    26041 ggccaatcgc ctgcgccagg ttaccagctt caacggcgat atccatggtg tgtttatctt
    26101 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    26161 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    26221 gaacacgcgc gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg
    26281 ttttcaccgc gattttcgca cgagaacccg ggtcgcgagc cgctgcttta atttcaatca
    26341 cttcttcgcc gatttccggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    26401 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaataa agcacgccac
    26461 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    26521 cttcagcgtt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    26581 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    26641 cacgcacttt ctgcacgata acctgttttg ccgtctgggt ggtgatacgg tcaaaggtaa
    26701 cagactcaat ctgatcttca acgtaatcgc ccaagttcag gctttcatct tcataacgtg
    26761 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    26821 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac acggacgtcg atctcttgct
    26881 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    26941 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    27001 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    27061 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    27121 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    27181 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    27241 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    27301 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    27361 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgcctacc agttcaaaac
    27421 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    27481 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    27541 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    27601 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    27661 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    27721 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc
    27781 tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    27841 aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat tgctaatcac
    27901 gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttccc ctgctctccc
    27961 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    28021 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    28081 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    28141 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    28201 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctgggccagc cagacgaaga
    28261 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    28321 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    28381 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    28441 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    28501 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    28561 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    28621 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    28681 ctccacagac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    28741 ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa
    28801 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggtgg cgctgaacgg
    28861 taaaaccttt agcggcgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    28921 cggcctgggc atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat
    28981 ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat
    29041 tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct
    29101 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    29161 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    29221 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    29281 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    29341 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tttcctcctc
    29401 tgccacttca ggcctgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    29461 caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    29521 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    29581 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    29641 ataaggtaat tattgtaagc tgtcgaaaag atattgtttt ccgttgtgcg atccacgcca
    29701 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctagcc
    29761 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    29821 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa
    29881 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    29941 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    30001 acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac
    30061 aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat actggtaact
    30121 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    30181 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacagaa
    30241 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct tattgcttga
    30301 ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga gcgtgtatat
    30361 ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca
    30421 atacttgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    30481 ctttgatgcg ctttcgctgc taaagcaaaa tagttgagat tgaaaaacgg cgtataggta
    30541 gcaaatcacg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    30601 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc
    30661 actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat
    30721 aaaaaagcaa aagcacatag ataagggata tacatcccca gcattttcac aacttcatcc
    30781 ggatcactct gcagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac
    30841 catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca
    30901 agcaggcgaa gcggaaggga tgatatacgt ttaacaacca ggatggtcag gacgaagaac
    30961 agcaatcgct gaaccatatg cgctccggaa gaggcgacta agttcgtaat accaaaaaca
    31021 ataacacaaa gatacaacaa ccaatgagat ttaaaatctt ctagcaaatt tgttgaatac
    31081 tgtcatccag aaatccaagt tcacaaccaa tataaacaat tatcaacaat gttcaatttt
    31141 atatcagaca tgtttttcaa ctctgatcca tatcagaccg aaacactgtt agttttttct
    31201 ttttaaatga tgcgaccatt gcgttctcag taattaaata acattatcaa tgcgtctcta
    31261 aacattacgc agaacgtagc catcagtagt tatactgaca ggagcaggat ggtttatcga
    31321 gaagtaaaca tatccgtatt cgtaccggaa ctggagttga cacaataaag tgccaattat
    31381 gtcagcagta gggaaaagtt tataactaag gcgtaccggc accatcgttt caaggtacca
    31441 gctacgagta aagcaactgg acgagataca gatacctgac aaccattcat cagaccagga
    31501 ccaaaacgaa aagagacgct tttcagcgtc tcttttctgg aatttggtac cgaggacggg
    31561 acttgaaccc gtaagcccta ttgggcacta ccacctcaag gtagcgtgtc taccaattcc
    31621 accacctcgg cacggatact actttttagt tcgggatatc gctggtcggc ttagccggag
    31681 cagctggctg agtttgttcg gttttcgccg gtgcactcag attttcccat tcgctacctc
    31741 tattggtttt gttgctattg atgttaccca gcaccagact gatgatgaag aataacgttg
    31801 ccagcagcgc cgtcatgcgg gtcatgaagt taccagaacc acttgaacca aacagcgtag
    31861 cggaagcgcc tgctccgaag gaggctccca tatcagcgcc tttaccttgc tgcagcatga
    31921 tcagaccaac aaggccaatt gccacaataa ggaaaactac taaaagagct tcgtacataa
    31981 tcaacctgtt ccttgcggag ttgccgcgta ccaatgcttc aaccaataaa gcgggagttt
    32041 ttaatctttc ccactgaagc gggtgtgaat actaaccaaa acgaatgacc ttcgcaaggg
    32101 caattttatc gcattgtatc aactgcggaa aaaaacagca aaaccgatgt gttcgccgag
    32161 aaaaaaggcg acaatcgccg cctttttagc cagttatcta acgctttaaa cggcttttac
    32221 tgcatcggcg atgcggtgtg caaattcagt cacctgcgct tcgtcttcgc cttccaccat
    32281 cacgcgaatt aacggttcgg tgccggattt acgcagcaac acgcgtccac ggttgcccag
    32341 cgcagcttca acctctgcgg tcacggcttt aactgactca tgctcaagcg gatcgccgct
    32401 acctgcggtg taacgtacgt taaccagaat ctgcgggaac attttcatgc cgctgcaaag
    32461 gtcgtgcagg ctcatatggt tacgtgccat cgccgccagc acctgcaagc cagcaacgat
    32521 gccgtcaccg gtagtggttt tatccagcag gatcacatga ccggaattct ctgcaccgat
    32581 acgccagcct ttctcctgca ttttttccag tacgtagcgg tcacccactt tcgcgcgcgc
    32641 aaatggaatt cctaactgtt tcagcgccag ttcaagcccc atgttgctca tcaatgtacc
    32701 cacagcacca ccacgcagct ggccctgacg aagaccttca cgcgcgatga tatacatgat
    32761 ctgatcgcca tcgactttat tgccttcatg gtcaaccata atcacgcgat cgccgtcgcc
    32821 gtcgaaggca ataccgagat ccgctttttc agccagcaca cgagcctgga gcgcgcgaac
    32881 gtcggtagcc cccacttcgg cattgatgtt tacaccgttt ggctcacaac cgatagcgat
    32941 aacgttcgcc cccagttcgc gcagcacgtt cggcgcgatg tgataagtcg caccgtttgc
    33001 acaatccacc acaatcttca gttcactgag gctaagttcg ttcgggaacg tggctttgca
    33061 aaactcgata tagcgacccg cggcatcaac gatacggctg gctttaccca gttcagccga
    33121 atcaacgcag ctgatctcct tttccatttc cgcttcgatg gcctcttcta ccgcatccgg
    33181 cagtttagtg ccgtcgatag agaagaattt aatcccatta tcgtagaacg ggttatgcga
    33241 tgcagatatc acaattccgg cctctgcgcg gaaggtacgc gtcagataag ccacggccgg
    33301 tgttggcatc gggccagtga agagtgcgga aaggcccgct gccgccagac ccgcttccag
    33361 tgctgactcc agcatatagc cagaaatacg cgtgtcttta ccaataataa tcttacggga
    33421 gccgtggcgc gccagcactt tacccgcggc ccaacccagc ttaagcacaa aatcaggtgt
    33481 gatcggcgca tcccctacac gaccacgaat cccatcggta ccgaaatatt tacgattact
    33541 catagcgttt gttttccttt gcagacagag tggcttccac cacccgcatc gcttctacgg
    33601 tttctttgac gtcatgaaca cgaatgatgt gcgcgccttg cattgcggca atgaccgcac
    33661 aggccagact accgctcagg cgctcggacg gccccacgtt cagcagctgc ccaatcatcg
    33721 attttcgtga catacccacc aacagcggca ggttgaaatg gtgaaattca gccaggcgcg
    33781 ccagtaatga atagttatgg gagagatttt taccgaaacc gaatccgggg tcgagcaaca
    33841 atttctcttt tgcgataccc gcctgctcgc aacgtgctat ttgctcaata aagtagcgat
    33901 tcacttctgc aaagacatcg tcatacttcg gagcttcctg catggttttt ggatttccct
    33961 gcatatgcat cagacaaacc ggtaaaccgg tttctgcagc cgcctccaga gcgccaggtt
    34021 cggaaaggga gcggatatca ttaataatgt gagcgccaac tttcgctgac tcacggatga
    34081 cttctggttt ggatgtatcg accgagatcc agacttcgaa gcgttgagca attgcctcaa
    34141 ccacaggaat aacacgttgc aactcttctt caacgctaac ttccgccgcc cctgggcgcg
    34201 tggactcgcc accaacgtca atgatcgtcg cgccagcatt gatcatcaga ttcgcatgtt
    34261 tcaccgcatc tatcagcgag ttatgcgtgc caccatccga aaaggaatca ggcgtgacgt
    34321 tgaggatccc cattacgtga ggatggctaa ggtccagtga agtaccctgg gcaaagagtt
    34381 tcattatgtt atccctggta tgaattgata agaaaaaacc ccggagcacg ccccggggtt
    34441 ttcggtacaa atacagtcat ctgatgcggg aacttacttg tcgcctaact gctctgacat
    34501 ggtgttaccc gggttcggcg tacgcggttc atcaaccgga cgaggagcct ttggactacc
    34561 attgtcgcca gaattgttag aagcgcctgg ttcttcccag cccgctggcg gacgtacatc
    34621 gcgacgtgcc atcaggtcat caatctgcgg tgcgtcgata gtctcatatt tcatgagagc
    34681 atctttcatc gcatgcagaa tatccatatt gtcggtcaga agctgacgcg cacgattata
    34741 gttacgctca atcagtgctt tcacttcctg gtcgatgata cgtgcagttt catcggacat
    34801 atgtttcgct ttcgctacgc tacggccgag gaatacttca ccttcttctt ccgcgtacag
    34861 cagtggaccc aatttctcag agaagcccca ctgagtcacc atgttacgtg ccaggttggt
    34921 cgcaacttta atatcgttgg acgcaccggt agaaacatgt tccggcccgt agatgatctc
    34981 ttctgccaga cgaccaccgt acagcgtaga aatctggctt tccagtttct gacggctggc
    35041 gctgattgcg tcgccctcag gcaagaagaa agtcacaccc agcgcacgac cgcgtgggat
    35101 aatcgtcact ttatgcaccg gatcgtgttc cggcaccagg cgaccgataa tcgcatgacc
    35161 cgcttcgtgg taagccgttg attctttctg cgcttccgtc atcaccatgg agcgacgttc
    35221 cgcacccatc atgattttgt ctttcgcttt ctcgaactca accatcgaca caacgcgttt
    35281 gttgccacga gcagcgaaca gtgccgcttc gttcaccagg ttcgccaggt cagcaccgga
    35341 gaaaccagga gtaccacgag caatgattgc cgcgtcgata tcgggtgcca atggtacgcg
    35401 acgcatgtga actttcagga tctgctcacg accgcgaaca tctggcaagc cgaccacaac
    35461 ctggcggtcg aaacggccag gacgcagcag ggccgggtcg agaacgtccg gacggttagt
    35521 cgcggcgata acgatgatac cttcgttacc ttcgaagcca tccatctcaa ccagcatctg
    35581 gttcagagtc tgttcacgtt catcgtgacc accgcccaga ccagcgccac gctggcggcc
    35641 tacggcgtcg atttcatcga taaagatgat gcacggtgcc gctttcttcg cctgttcgaa
    35701 catgtcacga acacgggatg cacccacacc gacgaacatt tctacgaagt cagaaccgga
    35761 gatagtaaag aacggaactt tcgcttcgcc tgcaatcgct ttcgccagca acgttttacc
    35821 ggtacccgga ggaccgacca tcaggacgcc tttcgggatc ttaccgccga gtttctggaa
    35881 gcggctcggc tcgcgcagat actctaccag ttcagcaact tcttcttttg cttcgtcgca
    35941 gcccgcaacg tcagcaaagg tcgttttgat ctgatcttcc gtcagcatgc gcgctttgct
    36001 cttaccaaac gacatggcac ctttgccacc gccgccctgc atttgacgca tgaagaagat
    36061 ccagacacca atcagcaaca gcatcgggaa ccaggagatg aagatagaag ccagcaggct
    36121 tggttcttca ggcggttcac cgacaacctt gacgttcttg gtcaacaggt tatccagtaa
    36181 tttcggatcc tgaaccggaa tgtaagtggt ataacggtta ctatctttct tggtaacgtt
    36241 gatttcacgt ccgttgatac gcgcttcacg aacctggtcg ttattgacct cttgtaggaa
    36301 ggtagagtaa tccaccttac ggccattaga ctcgctgggc ccaaagctct ggaatactga
    36361 catcagcaca acggcaatga ccagccagag tattaggttt ttcgccatgt cactcaaggg
    36421 attaacctct tattacaact gtgttaaaaa cagcgtcagg atactctata tccagcatct
    36481 ttcaaacttt cgtctgaaat ctcccggtta gggtttacgc ccggtcgcta caatatacac
    36541 ttctcgcgaa cgtgcacgag aagagtccgg cttacgaact ttgaccttcg taaacaggga
    36601 gcgaatttcc cttagatact catcgaaacc ttcgccctgg aacaccttca ctacaaaact
    36661 gccacctggc gctaatacat cacgacacat ttctagcgcc agttccacca gatacatggc
    36721 tcgggggata tccaccgccg gtgttccgct catgtttggt gccatatcgg acatgacaac
    36781 ctggactttg ctgtcgccaa cgcgctccag cagtgctttc atcaccagtt catcacgaaa
    36841 atcgccctga agaaagtcca caccaacgat aggatccata ggtaaaagat cgcaagcgat
    36901 gatgcggcct ttgccgccaa tttgggtgac cacatattgt gaccaaccac ccggagcagc
    36961 accaaggtcg acaaccgtca ttcccggttt aaagagtttg tcactttgct gtatttcatc
    37021 aagtttaaac caggcacggg aacgtaatcc ctttttctgt gcctgttgaa catatttatc
    37081 gctaaagtgt tcctgaagcc agcggctgga gctggcagaa cgcttcttac ctgtcattta
    37141 actttcccat ggggataact catcgtaacc aattgcgtaa atttttacgc gcctatttgg
    37201 tgatatatgg gagatggcgg tagaatgacc cgttttcaat cccaacgtaa gcaaaaaata
    37261 cgatgaatct gagtactaaa caaaaacagc acctgaaagg tctggcacat ccgctcaagc
    37321 cagttgttct gcttggcagt aatggtttga ccgaaggggt gctggccgag attgaacaag
    37381 cgttagagca ccatgaactc atcaaggtga aaatcgccac cgaagatcgc gaaacaaaaa
    37441 ccttgatcgt ggaagctatc gtgcgcgaaa ccggcgcctg taatgtacag gtcatcggta
    37501 aaacgctggt gctttatcgc ccaactaaag aacgtaaaat ctcgctgcca cgctaagatt
    37561 atcctaaagt tacacacatt tgctgtgtaa aacgaggggt tttccgcagg caggagagca
    37621 aaatgccacg ctctgttcgt tgataaaagg ccgcagagcg gcctttttcc tttctttaca
    37681 atacatcaac atcttgagta ttgggtaatt cttacaggta ttccacctta attacttcaa
    37741 attctacttc gccgcccggc gttttgatga ccacaacatc atcttcttct ttgccgatca
    37801 ggccacgagc aataggcgag tttacagaaa tcaggttttg tttaaagtca gcttcgtcat
    37861 cgccaacgat gcgataagtc tgttcttcgt cagaatccag attcagcacc gttacggtag
    37921 caccaaaaat aacgcgccca ttgttgggca ttttggtgac atcaatcacc tgcgcgtttg
    37981 acagcttggc ttcgatgtct ttgatacggc cttcgcagaa gccctgctgt tcacgagctg
    38041 cgtggtattc ggcgttttct ttcaggtcgc catgctcacg cgcttccgcg atagcagcaa
    38101 tgatttcagg acggcgcaca gatttcagaa aatccagctc ttcgcgtaat ttttcagcgc
    38161 cgcgtaaggt catcggaata gcttgcattt gttatacctc ttgaatattc ctgatggggc
    38221 aagtcttcac ccaccccggc tgttcggcct gcccggcata actcctcacc gggaccagaa
    38281 gcaaaaaaat accgacccgg gtacaagtcc caggtcagct acaattcaca ttttgatggt
    38341 cattttaccc tgaagttccc gaagggtcat cgtttacttt atagggcgtt gcgccgtagt
    38401 atgacggctc gattccaggt tgttagcgcg agattatgcg attttccaga tttatcatcg
    38461 gattgaccag ctgtatagcg ttcagtgttc aggccgcgaa tgttgatgag tacgttactc
    38521 aactccccgc aggtgccaac cttgccctga tggtgcaaaa agtcggtgcg tcggcccccg
    38581 ctattgatta ccacagtcag cagatggcgc tgcccgccag tacccagaaa gtgattactg
    38641 cgctggcggc gttgattcaa ctcggccccg attttcgttt caccacgacg cttgaaacca
    38701 aaggcaatgt ggaaaacggc gtacttaagg gtgacttagt ggcgcgattt ggtgccgatc
    38761 cgacgttaaa acgtcaggat attcgcaata tggtcgcgac tttgaaaaaa tctggcgtca
    38821 accaaatcga tggcaatgtg ttgatagata cctccatttt cgccagccac gataaagccc
    38881 ccggctggcc atggaatgac atgacacaat gctttagcgc tccgcctgct gccgccatag
    38941 ttgaccgcaa ctgtttctcc gtctcgctct acagtgcccc aaagcctggt gatatggctt
    39001 ttatacgcgt ggcatcttat taccccgtta cgatgttcag ccaggtacgc accctccccc
    39061 gtggttctgc cgaagcgcaa tactgcgaac tggatgtggt gccaagcgac ctgaaccgct
    39121 ttacgctgac gggatgcctg ccacaacgtt ctgagccgct cccgttggct tttgccgtgc
    39181 aggatggagc cagctatgcc ggtgcaattc tgaaagatga gttaaaacag gcgggtatca
    39241 cctggagcgg aacactgctg cgccagactc aggttaacga acctggaacg gtagttgcca
    39301 gtaaacagtc ggccccgctg cacgatctgc ttaagattat gctgaaaaag tcggacaaca
    39361 tgatcgccga tacggttttc cgcatgatcg gccatgcgcg cttcaatgtg cctggaacat
    39421 ggcgggctgg gtcggacgcc gtgcgtcaga tcctgcgcca gcaagccggt gtcgatattg
    39481 gtaacaccat tattgccgat ggttcagggc tttcgcggca taacctgatt gcccccgcca
    39541 ccatgatgca ggtgctgcaa tacattgccc aacacgacaa tgaacttaac tttatctcca
    39601 tgctgccgct tgcgggctat gacggctctt tgcagtaccg tgcaggtctg catcaggcgg
    39661 gcgtggatgg caaagtctca gcgaaaaccg gttcgttgca gggggtatat aacctggcgg
    39721 gattcattac cacagcgagc gggcaacgaa tggcatttgt acaatatctt tctggctatg
    39781 cagtaaaacc cgcggatcag cgtaatcgcc gtattccgtt agtgcgtttt gaaagccgtt
    39841 tgtacaaaga tatttatcag aacaattagt caaaaagaaa ccccggcgca tggccggggt
    39901 ttcagattat tgacaaagtg cgctttgttt atgccggatg cggcgtgaac gccttatccg
    39961 gcctacaaaa tcgtgcaaat tcaatatatt gcaatcatct tgtaggcctg ataagtgtag
    40021 cgcatcaggc ttatttggca ttggtcatta gtgattaacg cttgtaaatg aactcaacgc
    40081 cttcttcgtc gtcttcgtcc cagtcgtcat cccagtcttc atcatcctct tcagcaatct
    40141 cttcaagctg ctgacggtga taatcatccc acatgaattc gactttctct ggctgtttcg
    40201 cttcttcagc ctgcacgacc gggttttcaa tgataaaggt catcacatcc cagcagagat
    40261 ctttcacgcc cagtccgctc gccgcagaga tcagataata tttatcttcc cagcccagcg
    40321 cttcagcgat cgctttcgct ttctcttcgg cttctgcctt atccagcaga tcgatcttgt
    40381 tgaagactaa ccaacgcggt ttcgccgcca gatcctggct gtatttttcc agctcgctga
    40441 taataatacg cgcgttttca accggatcgg tgccgtcaat cggatcgata tcgatgaggt
    40501 gcaacaggac gcggcaacgt tccaggtgct tcaggaagcg aatgcccaga cctgcgcctt
    40561 ccgcagcgcc ttcaatcagt cctggaatat cggcaacaac gaagctcttt tcgttgtcca
    40621 ttcgtaccac acccagactt ggcaccagag tggtaaacgg ataatccgcc actttcggtt
    40681 tagccgccga taccgcacga ataaaggtcg atttacccgc gtttggcatc cccaacatac
    40741 cgacgtcagc cagcagcatc agctccagca gcagctcacg cttatcgccc ggcgtaccgt
    40801 tagttttctg ccgtggtgta cggttaactg acgatttgaa acgggtattg cccagaccgt
    40861 gccagccgcc cttagcaacc agcagacgct gaccgtgttt agtcatatca cccatggttt
    40921 caccagtacc ctggtcgatt acacgcgtac ctaccggcac tttaatcgtc acgtctttac
    40981 cgcgcttacc ggtacaatcg cggcttgcgc cgttctgacc gcgctctgca cggaaagatt
    41041 tttcaaaacg ataatcgata agcgtgttca ggttctcgtc ggcttccatc catacgtcac
    41101 caccatcacc gccgtcgccg ccatccgggc cgcctttcgg aatatacttt tcgcggcgga
    41161 agctcacgca accattaccg ccatcacctg caacgaccag aatcgatgct tcatcaacaa
    41221 acttcatttt attctccgta aatcattcgc ctgcgcgggg ttgcgatacc accgttgtat
    41281 gcttacgtaa tccgccccaa atacgatgac caatggcgga atacatcgcg cccgcaacca
    41341 cgacaaacgc accgagataa cctaaaaggt ttaacatcgg tctggcgaag aaatcgggcc
    41401 aggccagtga taaaagatct gaaaaaaaca gcgtaaacag tggggtgagc gtgatgatcg
    41461 cgctcacctg cgctgcctgc cagcgagcca tcgcttccgc caaggcgcca tatcctacca
    41521 aggtattcag tccgcaaaaa attaaacatg cgagctgcca gtggctaagc tgcgctatca
    41581 ctccaggctt tgccagaggg aagagcgcga ttgtacataa agtgtacagt aaaaacagga
    41641 tctgcggtga ggccagccga cgcaataaaa ccttttgcgc cacgccataa ctcacccaaa
    41701 ccgtcgccgc accgacccca aagataactc cccaggtgta atcggtgagc tttgtaaata
    41761 tctcgaccag actggtgtta aaaaacatca ccagaccgct caggagcatc aatgccccta
    41821 caacctgagt gctgcgcatt ttctctttca ggataaatac gctggcaacc atcatgccaa
    41881 ctggcgagag ttgcccaatc acctgcgaag cggtcggact caggtattgc aaggatgagc
    41941 tgaacaggat gaagttccca aacagcccgg cggtcgccac tgccaacaaa atcaaccagc
    42001 gtggcttacg aaacacgcgt aatggcggca acctcttctt caccgcaaga atggcaccaa
    42061 ggccaatact cgccatcaag aaacggtaaa acacgattgt cggaggttcc atcacctcca
    42121 gcacctgctt cattgcgatt ggcaacgccc cccagcaaat tgctgtggta agcgccaaaa
    42181 gaatgccaat gcctgcctgc tgcttcatac cgtattccct gcaaagcgca tttgcggatt
    42241 aacgtcgcac catgcgcatt tttaccggtt atcgaatgta aaaagccccg caacgtgttg
    42301 cggggctttc atccgttacc gggacgcgaa aaacttattc agcttcgatg ctgataaatt
    42361 tacggttttt cgggccttta acttcgaatt tcactttacc gtctgcttta gcaaacagag
    42421 tgtggtcacg accgcaacct acgttagcgc cagcgtggaa tttggtacca cgttgacgaa
    42481 cgatgatgct acccgccaga acggattcgc caccgaaacg cttaacgccc aagcgtttag
    42541 cttctgaatc gcgaccgtta cgtgtggagc cgccagcctt tttatgtgcc atttgaaatc
    42601 tctcctcagg tcttaggcgc tgatgccagt aattttcaca tcagtgaacc actgacgatg
    42661 gccctgctgc ttacgatagt gtttacgacg acgaaactta acgattttaa ctttctcgcc
    42721 acgaccgtga gcaacaactt cagctttgat tacgccgcca tcaacgaaag gaacgccgat
    42781 tttgacttct tcaccgtttg cgatcatcag cacttcagcg aactcaacag tttcgccagt
    42841 tgcgatgtcc agcttttcca ggcgaacggt ctgaccttcg cttactcggt gttgtttacc
    42901 accactttgg aaaaccgcgt acatataaaa ctccgcttcc gcgcacacct tttcaatgat
    42961 tcagagtgcg ctataaatat tcacaatagg gcgcgaatat tacgcaaaac gcacgccttt
    43021 gacaagtgct acagtcaata cacgaagaaa aaaacacaac ttgtacggta acgtttatct
    43081 gtgccatttt ttcagtacaa ttaccctata ttcctaacca taaaccctaa gttgcctttg
    43141 ttcacagtaa ggtaatcggg gcgaaaagcc cggcttttgc gatgaattta gaaaaaatca
    43201 atgagttaac cgcgcaagat atggcgggtg ttaatgcggc aatccttgag cagcttaatt
    43261 ccgacgtcca actgatcaat cagttaggct attacatcgt cagcggcggc ggtaaacgta
    43321 ttcgtccgat gattgctgta ctggctgcac gagctgttgg ctatgaggga aatgcgcatg
    43381 tcaccattgc tgccctgatc gagtttatcc acacggcgac tctgctacac gacgacgttg
    43441 tggatgaatc agatatgcgc aggggtaaag ctaccgccaa cgccgcattt ggcaatgccg
    43501 ccagcgtgct ggtaggcgat tttatttata cccgcgcgtt ccagatgatg accagcctcg
    43561 gttcactcaa agtgctggaa gtcatgtcag aagctgtaaa cgtcatcgca gaaggtgaag
    43621 ttctgcaact gatgaacgtt aacgatccgg acatcactga agaaaactac atgcgcgtta
    43681 tctatagcaa aaccgcgcgt ctgtttgagg ctgccgcgca gtgttccggg attctggctg
    43741 gctgtacgcc ggaggaggag aaaggcctgc aggattatgg gcgctatctc ggcactgctt
    43801 tccagttgat cgacgattta ctcgattaca atgccgatgg cgaacagtta ggtaaaaacg
    43861 tcggcgacga tctgaacgaa ggtaaaccga cgctgccgct gctgcatgcg atgcatcatg
    43921 gcacaccaga acaggcacag atgatccgta ccgccatcga acaaggtaac ggtcgccatc
    43981 ttctggaacc ggttctggaa gcaatgaacg cttgtggatc tcttgaatgg acgcgtcagc
    44041 gcgccgagga agaagcagac aaagccatcg cagcgttaca ggtgctcccg gacacccctt
    44101 ggcgagaagc actcatcggc ctcgcgcaca tcgctgttca acgcgatcgt taatcccctc
    44161 ccttcatccc gcgcagtgcg cgggatgatt ccagtaaatt ccataaaaac actaattcag
    44221 cgctataaca cctgaaaaaa gggcgatgaa tctttatata ttctgaatat tcacactctt
    44281 tacaggaact ttttagcgca atagaccatc aggagtatag tgatgcccga cagaagaagt
    44341 gttctgaatg aaagcgacta acttaaggag tgaggaaaga tggaaagtaa tttcattgac
    44401 tggcatcccg ctgacatcat tgcaggtttg cgcaagaaag gaacgtcaat ggcggcggaa
    44461 tctcgcagaa atggtttgag ttcctcaacg ctggcaaacg cattatcgcg cccatggccg
    44521 aaaggagaga tgattattgc gaaagccctg ggaactgacc cctgggttat ctggccatca
    44581 cgctaccatg atccgcagac ccatgagttt atcgacagaa cgcagttgat gcgtagctac
    44641 actaaaccga aaaaatgagt ggtctggcgg tagccccgcg aacggggctg ccagctctca
    44701 gacgattact cgcctttcac acgctcaata tttgcaccta aagcgcgcag tttgtcttca
    44761 atgcgttcgt agccacgatc gatgtgataa atacgatcaa ccaccgtcgt cccttccgca
    44821 atacagccag ccagcaccag gcttgctgat gcacgcagat cggttgccat aacctgtgca
    44881 ccagaaagtt tttcaacacc atgacaaata acggtattgc tttcgatttc ggcgtgcgcg
    44941 cccatacggc tcagctctgg cacatgcata aagcggtttt caaagaccgt ttcggtgatg
    45001 aacccggtcc cttcagccac caggttcaac agcgtgaact gggcctgcat atcggtcggg
    45061 aatgccggat gcggcgcggt acgtacgtta acagccttcg gacgtttgcc atgcatatcc
    45121 aggctaatcc agtcttcgcc gacttcgatg tccgctccag catcacgcag tttcgccagc
    45181 acggcgtcca gagtatctgg ctgcgcgtta cggcagataa ttttgccacg agagatcgcc
    45241 gccgccacca ggaaagtacc ggtttcgata cgatccggca gcacgcgata gacaccgccg
    45301 cctaaacgtt ccacaccttc gatgacgata cgatcggtgc cctgaccgct aattttcgca
    45361 cccagcgtaa tcaggaagtt cgcggtatcg acgatttccg gttcacgcgc tgcgttttca
    45421 ataatcgtgg tgccttccgc cagggttgca gcacacatga tggtcaccgt tgcgccaacg
    45481 ctgactttat ccatcacgat atgtgcgcct ttcagacgac catcgacgga agctttaacg
    45541 taaccttctt ccagtttgat ggtcgcgcct aattgttcga ggccagaaat gtgtagatca
    45601 accggacgcg caccgatcgt acaaccgcca ggtagtgaaa cttgcccctg accaaagcgc
    45661 gctaccagcg gccccagcgc ccagatagaa gcacgcatgg ttttaaccag atcgtaaggt
    45721 gcgcagaata cattaacgtc gcgggcatca atatgcacag aaccattacg ttctactttc
    45781 gcacccagct ggcttagcag tttcattgat gtatcgacgt ctttcagttt cgggacgttc
    45841 tggatctcta ccggttcttc cgccagtagt gcggcaaaaa gaataggcag agcagcattt
    45901 ttagcgccgg aaattgtgac ttcgccctgg agcttcgttg gcccctgaac acgaaattta
    45961 tccatttagt ttgttctcag ttaacaattc atatccgcta ccggcgaatc gcccatagct
    46021 caaaagccgt tcagtttgcg atcgcgcgcc cactccgcag gggtatacgc tttgatcgac
    46081 acagcatgaa tgcggttatc cgcaatatat tccatcagcg ggccatagac cgtctgctgt
    46141 tttttaaccc gactcatacc gtcaaacaac tcacccacgg caataacctg aaagtggctg
    46201 ccatcgccgg aaacgtggac ttcctggagg gagagagcgt tcatcaacac gctctgaatt
    46261 tcattatttt ccatgggatc ttcaatcatc agttaataaa ccagcgaaac atcttagagc
    46321 aaagttgcgc tggcataaat aagcaaaaag cctcgctgat aaatcagaca aggctcgact
    46381 tgcaggcagg tttgccggac aggcggttaa cgccatatcc ggcctgaaaa aatttaacga
    46441 ggcagaacat cagcaggcaa attatacaat ttcgcgaggg tatacacttt gtcgtttacc
    46501 ccctgaagcg tcacgttgtt gccctgcttt ttcgccagat cgataagatg gagcagcagt
    46561 gccagtcccc ccgtatccac gcgggacaca cggctaagat cgatgcaggt aatccccttc
    46621 accgcctcct cacgcatttc ccaaagcggt agcagaacgt cctgatccag ctctccggat
    46681 aacgccagcg tgtcacccgt ctgcatccag ctcagtgact cgctcattat tttttctctt
    46741 ccagagtgat tttctgttga gaaatcgatt tcagttgcgc agtcaggccg tcgatacctt
    46801 tggtacgcag cagcgttccc cactcgtttt gtttggtggt gatcatactg acgccttcag
    46861 caatcatgtc gtaagcttgc cagtttcccg tctgggagtt tttacgccac tggaagtcca
    46921 gacgcaccgg cggacggcca ttcgggtcaa taatggtaac gcgaataggc acaatggttt
    46981 tatcgcccag cggctgttct ggcgcaatct gataggtttg accgtgatac atcgccagcg
    47041 cctgaccgta agcctgcttc aggtactcac ggaaagcggc aaagtaggct tcacgttgag
    47101 caggggtcgc actcttgtaa tactggccca gcaccagcgc accggcgtat ttcacctgta
    47161 cgtatggcag cagttcctga tcaacaatgg tacgcagata atccgggttg gcccgaattt
    47221 gcggttgctc attcttcagg cgatcgaacg ttttctgcgc cgcctcgtcc atcagcttat
    47281 aaggattggt ctggtctgcc gcggttgccg cactcagagg tgcaatcacc agcaaagcga
    47341 ccatcattaa acgtttaaac atgcgtcggt tctcctgaaa ttatttcgtt gtacccacag
    47401 gttcagtggt ttcattatta cctggcgcag cagctggcgc atcgccacta ttcttattgt
    47461 catcgccttt actaccgtaa aggaactgac caatgagatc ttccagcacc atcgcagact
    47521 tggtgtcctg aattgtatcg ccatccttca ggatagcagt ccccagttcc gggtcttcaa
    47581 aaccgacgtt taatgccaga tattgttctc ccagcaggcc ggaagtacga atgctcagcg
    47641 aactggtatc aggaatgtgg ttataacgtt gttcaatttc cagcgttacg cgcggcagat
    47701 aggttttcgg gtccagcgta atatccgcca cccggcccac aacaacacca ccaatgctga
    47761 ccggagagcg cgctttcagg ccgccaatgt tatcgaacgt cgcataaagc gtgtaggtcg
    47821 attcagtacg tatggacgtc acgttcgccg ccttcaggca aacaaacagc gccgccagca
    47881 gtgctgctaa taaaaagata cccacccaaa tttcattttt tttcgtttgc atgaactcaa
    47941 ttcccaaaca tcaatgcggt cagcacaaaa tccagcccca gaacagccag agacgagtgg
    48001 acaacggtgc gagtggttgc ccggctaatc ccggcagacg tcgggatggc gtcgtagccg
    48061 ttaaacaacg aaatccacgt caccgtgatg gcgaacacca cgctcttaat cagacagttg
    48121 accagatcca tacgccagtc gacggcattt tgcattgccg accagaagaa cccgctatca
    48181 atgcctttcc aactgacgcc gaccagcgat ccgccccaga tccccacggc gacgaagata
    48241 accgtcaaca gtggtaatga aataacccca gcccagaaac ggggagaaat aacccgacgc
    48301 agcggatcca ccgccatcat ctccatactg gagagttgct ctgtagcgcg catcaggccg
    48361 atttctgcgg ttaacgccga accagcacgc ccggcaaaca acagcgcggc aaccaccggc
    48421 cccagttcac gcagtagcga taacgccacc agcataccca gactggtttc cgcgctataa
    48481 gtggtcagaa ccagataacc ttgcagcccc aacaccattc cgatgaacac gccagaaact
    48541 acaataatca gcatcgacag gacgccgaca ttatagagct ggcgcaccag cagcggcgca
    48601 tgtttgcgaa attccggttt gccgaccagc gcattgaata acattaaccc ggcccgcccg
    48661 aacgttctca gggttttaat ccctttatgt ccgagcgacg ccagcgcatt taacagcatg
    48721 agtggcttaa ctccctggta aaagatcagc gtgataatcg ccggcaggat agcggaacgg
    48781 aacaggcccg tcagctatcc cgtccagaaa ctgacgtacg cgcggatcag gattcgtctg
    48841 caacgcctgg gcactgccat gagcgacaat ttttttgtcc gccaggatcc aggcgtgatc
    48901 cgcaatactt aacacttccg gcacatcgtg agaaaccacc acacaagtca cgcccagcgc
    48961 gctgttcagc tcagaaatca gcttcaccag tacgcccatg gtgatgggat cttgcccaac
    49021 aaaaggttca tcaaacatga tgagatccgg ctccagcgca atcgcacgcg ccagcgctgc
    49081 acgccgcgcc atcccaccgg aaagttcaga aggcattagt ttagccgctc cacgcagccc
    49141 cacggcctcc agcttcatca tcaccgtact atgcaacaat ggcgcgggaa gttgggtatg
    49201 ttcgcgcagt ggataggcga cgttgtcaaa tacgttcata tcagtgaaca acgccccgga
    49261 ctgaaataac atgctcatcc gtttacgcac tgtatacagg cgcgaacgag acatcgccgg
    49321 aatattctca ccatcgaaaa ggatctcacc atgatctggt gcgatttgcc cgccaatcag
    49381 acggagtagc gtcgttttac cgatgcccga tggccccatg atcgccgtga tcttccctcg
    49441 cggcacggtc agggaaatat tatcgaagat gcagcgattg ccacgcgtaa aactgacatc
    49501 gcgcatatcg actaaattcg ccacagactg ctccataatt cacccttcgt cttgcgttga
    49561 ttttctaagc atggcgctca atttaacctt gaacccaaca tatttacaga atattacccg
    49621 ccgtggttag cgaaagctgg catttgtttt actttttagc cgcataaagt caaaattaag
    49681 aatccgttac ggctttctga aaatcttcag cggaccggcg agtatacctg aagaaaggac
    49741 gttagatgct tttagctacg gcactgttaa ttgttggttt acttttggtc gtttacagtg
    49801 ccgaccgcct ggtttttgcc gcgtctattc tttgccgaac ctttggcatc ccgccgctga
    49861 tcatcggcat gacggtggtc agtattggta catcgttacc agaaatcatc gtctcgcttg
    49921 ccgcgtctct gcacgaacaa cgcgatttag ccgttggtac agccctcggc tcaaacatta
    49981 tcaatatatt gctgatcctc ggtctggctg cgctggttcg tccttttacc gtccattccg
    50041 atgttctgcg ccgtgaatta cccttaatgt tgttggtcag cgtggtggcc ggttccgtac
    50101 tctatgacgg acaacttagt cgaagcgatg gtatctttct cctctttctg gctgtgctat
    50161 ggctgctgtt cattgttaaa cttgcacgtc aggggactga cagcctgacc agagagcagc
    50221 ttgcagagct gccgcgtgac ggcggattgc ccgtcgcgtt tttatggctc ggcattgcgc
    50281 ttatcatcat gccagtggcc acgcggatgg tggttgataa cgccacggtg ctggcgaatt
    50341 actttgccat cagcgagttg acgatggctc tgacggcaat tgctatcgga accagtctgc
    50401 cggaactggc aaccgcaata gcgggggttc gcaaaggtga aaacgacatt gctgtcggaa
    50461 atatcattgg cgcaaacatt tttaatattg tcatcgtgtt gggtttaccc gcgctgataa
    50521 cgccaggaga gattgatcca ctggcgtaca gtcgtgacta cagcgtgatg ttgctggtga
    50581 gcattatttt tgcgttgctg tgctggcggc gctccccgca accgggccgt ggtgtagggg
    50641 tattattaac tggcggattt atcgtatggc tggcgatgtt gtactggtta tcgccaatac
    50701 tcgttgaata actggaaacg cattatgtcg cacgtagagt tacaaccggg ttttgacttt
    50761 cagcaagcag gtaaagaagt cctggcgatt gaacgtgaat gcctggcgga gcttgatcaa
    50821 tacatcaatc agaatttcac gcttgcctgt gaaaagatgt tctggtgtaa agggaaagtt
    50881 gtcgtcatgg ggatggggaa atcggggcac atcgggcgca aaatggccgc aacgtttgcc
    50941 agcaccggta caccttcatt tttcgtccat cctagtgaag ccgcgcatgg tgatttaggc
    51001 atggtcaccc cacaggatgt ggtgattgct atctctaact caggtgaatc cagcgaaatc
    51061 acggccttaa ttccagtgct taagcgtctt cacgtaccgt taatctgcat caccggtcgc
    51121 ccggagagca gcatggcgcg cgccgcagat gtgcatctgt gtgttaaagt agcgaaagaa
    51181 gcctgtccgt tagggctggc accgaccagc agcaccaccg ccacgctggt tatgggcgat
    51241 gccctcgctg tcgcgctgtt aaaagcacgc ggctttactg ctgaagattt tgcgctctca
    51301 cacccaggcg gcgcactggg tcgtaaactt ctgctgcgcg taaacgatat tatgcatacg
    51361 ggcgatgaga tcccgcatgt taagaaaacg gccagtctgc gtgacgcatt gctggaagtt
    51421 acccgcaaaa atcttggtat gactgtcatt tgcgatgaca atatgatgat tgaaggcatc
    51481 tttaccgacg gtgatttacg ccgtgtcttc gatatgggcg tggatgttcg tcagttaagt
    51541 attgccgatg tgatgacgcc ggggggaata cgtgtgcgcc ctggcattct ggccgttgag
    51601 gcactgaact taatgcagtc ccgccatatc acctccgtga tggttgccga tggcgaccat
    51661 ttactcggtg tgttacatat gcatgattta ctgcgtgcag gcgtagtgta aagattcaag
    51721 gataaacaac aatgagcaaa gcaggtgcgt cgcttgcgac ctgttacggc cctgtcagcg
    51781 ccgacgttat ggcaaaagca gagaacattc gtctgctgat cctcgacgtt gatggcgtac
    51841 tgtcagatgg cctgatttat atgggcaata acggcgaaga gctgaaagcg ttcaatgttc
    51901 gtgacggtta tggcattcgt tgtgcgctca cctctgatat tgaagtcgct atcattaccg
    51961 ggcgaaaggc taaactggta gaagatcgtt gtgccacatt ggggatcact cacttgtatc
    52021 aggggcagtc aaacaaactg atcgccttta gcgatttgct ggaaaaattg gcgattgccc
    52081 cggaaaatgt ggcttatgtt ggcgatgatc tcatcgactg gccggtaatg gaaaaagtgg
    52141 gtttaagcgt cgccgtggcc gatgcgcatc cactgttgat cccgcgcgcc gattacgtga
    52201 cgcgcattgc tggcggtcgt ggcgcagtgc gcgaagtttg cgacttatta ctcctggcgc
    52261 agggcaaact ggatgaagcc aaagggcaat cgatatgagt aaagccagac gttgggttat
    52321 cattgtgcta tcactggcgg ttctggtgat gatcggcatt aatatggccg aaaaagacga
    52381 taccgcccag gtggtcgtca acaacaatga tcccacctat aaaagcgagc atacggacac
    52441 gctcgtctat aacccagaag gggcactaag ctatcgattg attgctcaac acgttgaata
    52501 ttattccgat caggccgttt cgtggtttac gcagccggta cttaccacgt ttgataagga
    52561 taaaatcccg acatggtccg taaaagcaga taaagccaag ctgaccaatg accggatgct
    52621 ctatttatat ggacacgttg aagtcaacgc acttgtgcca gactctcaac ttcgcagaat
    52681 cacgacggat aacgcgcaga tcaatctggt gacgcaggat gttacctctg aagacctcgt
    52741 cacgttatac ggaacaacat ttaactccag cggtctgaaa atgcgcggca acttacgcag
    52801 caagaacgcc gagctgattg aaaaggttag aacatcctat gaaattcaaa acaaacaaac
    52861 tcagccttaa tcttgtgctt gccagctcac ttctggccgc cagcattccg gcatttgccg
    52921 taaccggaga cactgatcag ccgatccaca ttgaatcgga ccagcaatct cttgatatgc
    52981 aaggcaacgt ggttaccttt accggtaatg tcatcgtcac ccagggcacc atcaaaatta
    53041 atgccgacaa agtggtcgtt acccgtccgg gcggcgaaca aggtaaagaa gtgattgacg
    53101 gatacggtaa accggcaacg ttctaccaga tgcaggacaa cggtaaaccc gttgaaggtc
    53161 acgcttccca gatgcactac gaactggcaa aagattttgt cgttctgacg ggtaatgctt
    53221 atctgcagca ggtcgatagc aacattaagg gcgataagat cacttacctg gtgaaagagc
    53281 agaaaatgca ggctttcagc gacaaaggca agcgcgtaac caccgttctg gtgccgtcgc
    53341 agctgcagga caaaaacaac aaaggccaga ccccggcaca gaagaagggt aattaattcg
    53401 ttatggcaac attaactgca aagaaccttg caaaagccta taaaggccgt cgcgtggtag
    53461 aagacgtcag cctgaccgtc aactccgggg aaattgtcgg tctgctgggg ccaaacggtg
    53521 ccggtaagac caccactttc tacatggttg taggcattgt gccgcgcgat gcgggcaaca
    53581 tcattattga tgatgacgat atcagtctgc tgcctctgca tgcacgcgcg cgccgcggta
    53641 tcggctatct gccacaggaa gcctccattt tccgtcgcct cagcgtttac gataacctga
    53701 tggcggtact gcaaattcgt gacgacttgt ctgctgaaca acgtgaagac cgcgcgaacg
    53761 agctgatgga agagtttcac attgagcacc tgcgtgacag catggggcag tcactctccg
    53821 ggggtgaacg tcgccgtgta gaaattgccc gcgcactggc tgcgaatccg aaatttattc
    53881 tgctcgacga accgtttgcc ggggttgacc cgatctcggt tatcgacatt aaacgcatca
    53941 ttgaacacct gcgcgacagc ggcctgggcg tgctgatcac tgaccacaac gtgcgtgaaa
    54001 cactggcggt ttgtgaacgc gcttatatcg tcagtcaggg gcatttgatc gcccacggca
    54061 cgcctacaga aatcttacaa gacgaacacg ttaagcgtgt ataccttggg gaagacttca
    54121 gactctgata gggtagaagt ttgcgacgtt ttagcaggag agtacgattc tgaacatgaa
    54181 gcaaggtttg caactcaggc ttagccaaca actggcgatg acgccacagc tccaacaggc
    54241 aattcgtctg ttgcagttgt cgacgctgga acttcagcag gagctacagc aggcgctgga
    54301 gagtaatccg ctgcttgagc aaatcgacac tcatgaagaa atcgacaccc gcgaaacgca
    54361 agacagtgaa acgctggaca ccgccgacgc gctcgaacaa aaagagatgc cggaagagct
    54421 gccgctcgat gccagttggg acaccattta caccgctggt acaccatccg gcaccagcgg
    54481 tgactacatt gacgacgagc tgccggtcta ccagggcgaa acgacgcaga ccttgcagga
    54541 ttacctgatg tggcaggtcg agctgacacc gttttccgac actgaccgcg ctattgctac
    54601 ctctatcgtc gatgccgttg atgaaaccgg ttatctgact gtcccgctgg aagatattct
    54661 cgaaagtatg ggcgatgaag agatcgacat cgacgaggtt gaagccgtcc ttaagcggat
    54721 ccaacggttt gacccggtcg gtgtagcggc aaaagatctg cgtgactgcc tgctaatcca
    54781 actctcccaa ttcgataaga ccacgccgtg gctggaagag gccagattga tcattagcga
    54841 tcatctcgat ctgttagcca atcacgactt ccgcacttta atgcgcgtca cgcgtctgaa
    54901 agaagatgtg ctgaaagaag ccgtcaatct gatccagtcg ctcgatccgc gccccgggca
    54961 gtcgatccag actggcgaac ctgagtatgt cattccagat gtgctggtgc gtaagcataa
    55021 cggtcactgg acggtagaac tcaacagtga cagcattccg cgtctgcaaa tcaaccagca
    55081 ctacgcctcg atgtgcaata acgcgcgcaa cgatggtgac agccagttta tccgcagcaa
    55141 tctgcaggat gccaaatggt tgatcaagag tctggaaagc cgtaacgata cgctactgcg
    55201 cgtgagtcgc tgtatcgttg aacagcagca agccttcttt gagcaaggtg aagaatatat
    55261 gaaaccgatg gtactggccg atatcgccca ggctgtcgaa atgcatgaat cgacgatatc
    55321 tcgcgtgacc acgcaaaaat acctgcatag tccacgaggc atttttgaac tgaagtattt
    55381 cttttccagt cacgtcaata ccgagggcgg cggcgaagct tcctccacgg cgattcgtgc
    55441 actggtgaag aaattaatcg cggcggaaaa cccagcgaaa ccgttgagcg acagcaagtt
    55501 aacctctttg ctgtcggaac aaggtatcat ggtggcacgc cgcactgttg cgaagtaccg
    55561 agagtcttta tccattccgc cgtcaaacca gcgtaaacag ctcgtttgac ccaaccgata
    55621 aggaagacac tatgcagctc aacattaccg gaaataacgt cgagatcacc gaggcactgc
    55681 gcgaatttgt tacagctaaa tttgccaaac ttgagcaata ttttgaccga atcaaccagg
    55741 tctatgttgt tctgaaagtg gagaaagtca cccacacctc agatgcaaca ctgcatgtaa
    55801 acggtggtga aattcatgcc agcgcggaag gtcaggacat gtacgctgcc attgatggct
    55861 taattgataa gctggcacgc cagttgacca aacataaaga taaactgaaa caacactaat
    55921 tgtccgggca attagcatgt gcatggcggc ctgttgtgcg gcacaacggg ccatttgtac
    55981 ggttaatgct ccgagcctgt tccactgttt gagtgggcag gttcttaggt gaaattatga
    56041 caaataatga tacaactcta cagcttagca gtgttcttaa cagggaatgt acgcgaagcc
    56101 gcgtccactg tcagagcaaa aaacgcgccc tggaaatcat cagcgagctg gcggcgaaac
    56161 aacttagcct gccgcctcag gtggtttttg aagctatcct gacgcgggaa aaaatgggca
    56221 gtaccggtat cggcaatggt attgccattc cgcatggcaa acttgaagaa gatactctgc
    56281 gtgccgttgg cgttttcgtt cagctcgaaa cgcctatagc tttcgatgcc atcgacaacc
    56341 aaccggtgga cttacttttt gccctgctgg tgccggcaga ccaaactaaa acgcacctgc
    56401 atactctgtc gctggtggcg aaacgtctgg cggacaaaac catctgccgc cgtttacgcg
    56461 cagcccagag cgatgaagag ctgtatcaaa tcattacgga taccgaaggt actccggatg
    56521 aagcgtagtt attcggtaat gtctctttta gacgttgtga ggagaaacag tacatggtac
    56581 tgatgatcgt cagcggacgt tcaggttcag gtaaatctgt cgccctgcgt gcgctggaag
    56641 atatgggttt ttactgcgtg gataaccttc ccgtagtgtt gttacccgat ctggctcgaa
    56701 ctctggccga tcgcgagatt tctgccgccg tcagcattga tgttcgtaat atgccggagt
    56761 caccagaaat attcgaacag gcgatgagta acctgcctga cgctttctca ccgcaactac
    56821 tgttcctgga tgccgaccgt aataccttaa ttcgtcgtta cagtgacacg cgccgactgc
    56881 atccgctttc cagcaaaaac ctgtcgctgg aaagtgctat cgacaaagaa agcgatttgc
    56941 tggagcctct gcgttcgcga gcggatctga ttgtcgatac ctcagaaatg tccgttcacg
    57001 agctggctga aatgctgcgt acccgtctgc tgggtaaacg tgaacgcgaa ctgaccatgg
    57061 tctttgagtc tttcggcttc aaacacggta tccctatcga tgcagattac gtctttgacg
    57121 tgcgcttctt gccgaacccg cactgggatc cgaaactgcg tccaatgaca ggtcttgata
    57181 aacctgtcgc cgcgttcctc gaccgccaca cagaagtaca caattttatc taccagacgc
    57241 gaagctatct tgagctatgg ttacctatgc tggaaaccaa caaccgtagc tacctgacgg
    57301 tcgccattgg ttgtaccggc gggaagcacc gttcggtgta tattgcagag caactggcag
    57361 actacttccg ctcgcgcggt aaaaacgtcc agtcacgcca tcgtacgctg gaaaaacgta
    57421 aaccatgacc gtcaagcaaa ctgttgaaat cacaaacaag ctgggcatgc atgcccggcc
    57481 tgcaatgaag ctgtttgaat taatgcaggg ttttgacgct gaagtgctgt tacgtaatga
    57541 cgaaggcacc gaggctgaag ccaacagcgt tattgcgctg ctgatgttgg attctgccaa
    57601 aggacggcag attgaagttg aagcgaccgg tccacaggaa gaggaagcac tggccgccgt
    57661 tatcgccctc tttaattctg gttttgatga agattaatct tcatcacttt gacatacaac
    57721 tcccttcaag ctcccccgac aataagaaaa tcacgtactg aaatcgttct caatcaacgt
    57781 catttgtaca ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac cttaagaggc
    57841 attgtttagg ttttgtttaa gttaatcgac catactggag atcgtcagaa aatatttcca
    57901 ggagatggca tgattcgctt atctgaacaa agtcccctgg gcaccggaag acatcggaaa
    57961 tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga ttgtctacca tcgtggcgat
    58021 ggtggcgata aagagatccg ccgtgagtta aagtactacg cgcatcttgg tcgccggtta
    58081 aaagactgga gtggaatacc gcgctatcac ggtaccgttg agacggattg cggaaccggt
    58141 tatgtctacg atgtgattgc cgattttgac ggcaagcctt ccattacgct gaccgaattt
    58201 gctgaacaat gccgttacga agaagacatt gcacaactgc gccagctact gaaacagctg
    58261 aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca gaatatcctc
    58321 tgtcaccgca tcagtgagtc agaggttatc ccggtcgtct gcgacaacat tggtgaaagc
    58381 acattgattc cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca agagcggtta
    58441 tggaaacgat ttatcgccca accggcgctg gctattgccc tgcagaaaga tctgcaacct
    58501 cgtgagagca aaacgctggc cctcacttcg cgcgaagctt aatccagctg gtgttgttgc
    58561 ataaacggct caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg acggctacgc
    58621 acgtagcccg atggcgagga gactttgaaa cgaagtggat taggtaatac agctgccagt
    58681 aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt ggaaataacg ttgtgccgca
    58741 gcttcgacgc caaacacacc gtcgccaaat tcggcgatat tcaggtaaac cgtcaggata
    58801 cgctttttgc tccagactgt ttctatcccc agcgttaatc cagcttccag cccttttcgt
    58861 acccagctac gcccatccca taaaaagaga ttttggccgt ctgctgagaa atcgttgaag
    58921 caccgcgaat acggttttca ttgcgctcgt tgtgcgccag ggctttctca atggaagcga
    58981 catcaaagcc ccagtgctca ggaaatttct gatcttctgc ggcaatcacc gccagtccca
    59041 tccacggcga gatttgatcc atactgaccc agtcagaatg tgccacgtaa cgaaaattgc
    59101 catgcagcca ggcgctgacc tgtcgctcga ccattaccgc tgagaaggga acaggcgcaa
    59161 cgctaaacaa cgcgatgccc ccgccccaga aaacggcgag gacaaccatt aaccgcaaaa
    59221 gaaaacggcg aacgaaacta aacaccgtta agcggctttt actcattcag ccagaaccag
    59281 cacgcgggaa accagcttat caatgccgct cgccgcttct gcaatgttct gcgccagcat
    59341 atatgctggg gtggtgacaa tcttattgtc ttcatcaacc acgatatcat cgacaggaca
    59401 cggcacatgc tccgcgccca tctcttccag cacttctgcg gtatcgatat cagtaccgat
    59461 ggtcaaacgc agcgggaaat cgaaaatttt cggcagcatc gccggggcaa tacacataaa
    59521 accaagcggt tttccggcct gatgcatcgc ttgtgccagc gcctttaatt cacggtcaac
    59581 ggtgcattcg ctaccaagac tggcaaaatt gcttaaattc ttcgccgcgc caaacccccc
    59641 cggcacaatc aacgcatcca gttcagcggc atcggcctgg gccagaggac ggatttcacc
    59701 gcgcgttatt cgtgccgctt caatcagcac attgcgcgtt tccgtcatcg cttcgccagt
    59761 taaatggttg ataacatcaa cctgctgctt atccggtgca aagcagaccg cctgcgcacc
    59821 gctgcgtgaa atagctaaca acgtcaacac cgcttcatga atttcagaac cgtcatagac
    59881 gccgcatccg ctcagaatta cgccaatttt cttcattgtg atcatccttt cgcaattgac
    59941 tgaaacacat taattttttt aataaaaatg gtacgcatca cacatttaac tgattcatgt
    60001 aacaaatcat ttaagttttg ctatcttaac tgcgtgcggc ctgaaaaaca gtgctgtgcc
    60061 cttgtaactc atcataataa tttacggcgc agccaagatt tccctggtgt tggcgcagta
    60121 ttcgcgcacc ccggtctagc cggggtcatt ttttagtggc ttttgccacc cacgctttca
    60181 gcacttctac gtcgtgacgc cactcttctt tcatctcttc aatccattca ccgacgttat
    60241 cttcccaggc gggaaggtca ggagactgaa tttgctgacc cagctgttgc agatggcgta
    60301 accccactga ccccgccgca cctttaattt tatgtccttc ctcaacaatg ccttttttat
    60361 cctgcgccgt cagattcgac tccagcacgc tgacatagcc cggcatcatc ttctcaaaca
    60421 ccgctaaccc gtcggtgatc agcttcggtc ctacaagttc gagatactgt tccagcatgg
    60481 gaatatcgag caatgcttct gatttactgt tctcttctgt cgtcaccgta ctctcctcat
    60541 catcctgggt atcccagaat ttcttgatca tcgcggttag cgccggaacc gaaagcggct
    60601 tactcagcac atcatccatt ccagcattga ggtactcttg tttgtctttc agcacgttag
    60661 cggttaaggc caccagcggc ggtaaatcct cgcgcggata acgcttcgtc agttcacgag
    60721 agatatccag cccggtcata tctggcaact gaatatccag caacaccagg tcgtattcgc
    60781 ccggtttaaa catctccagc gccgccttgc cggtcatggc gacatcaacg ctgttaccta
    60841 atttttccag cacagaacgc gcgacaatca cgttcagttc aatgtcttcc accagcagca
    60901 cattcagcgc cggtaaaggc atatcgtctt catcaaacgc atcatcgacc tcttctgcca
    60961 ccgacggtgc gtggatcgtc aacgtaaagg ttgaaccttt gccctgttca ctggtaaccg
    61021 taatatcgcc gcccatattt ttcgccagac gacgagaaac ggccagacca ataccggtgc
    61081 cggtggcagg tttaccgcca tgactgtctt tcacctggta atacatggcg aagattttat
    61141 ccagctcatc ctgcggaatg ccgataccag aatcttccac ttcaaaatgc agcatatcgc
    61201 cttcatcgta gcgcacgcgc acggtaacct ggccttgctg ggtgaatttg acggcgttac
    61261 tgatgaggtt ccacaggatc tgccgtaaac gcgtcccgtc ggtaatgacc tgatgcggta
    61321 atggcagcgt aggctccagg ttaaagcgca atcctttttg ttgcgcctgc aatgcggaga
    61381 gattttccag atcggcaagg aagctggtga aatcaaccgg ctgattatca agctggacct
    61441 tgcgccgttc catcttatcc atgtcgataa tatcgttaaa gatattcccc agcgtgacgg
    61501 ccgaaacatg gatagttttg agatattttt cctgctcggc ggtgagttcg gtatccagca
    61561 gaatgcggct caggccgacg ataccgttca gcggtgtacg caattcgtga ctgatggtgg
    61621 aaataaacgt cgttttgtcg cggctggcgc gttcaagcgc atcctgatac cgcttacgct
    61681 cggtaatgtc gcgaccaaag cccatcaaac cgtgacgttt acccacgcgg tcgtagtacg
    61741 gcactttacg gatttcaaag caggctttgc gcccgtccgg gtaatccagc cactgttcat
    61801 aggtcagtga cacattatga cggaacactt tttcatcggt ttcaatgact tttgcggcgg
    61861 cttccggtga gtaaacatca gcaggtttca ggtgaaccag ttgtttttcg ctttttccgg
    61921 tcagcagctc catcgcgcgg ttacagccgg aaaactcttt atcttcgtta cgataaaaaa
    61981 ccaagtcggg tgaagcatca aggaaggaac gtaagaatga ggattgctgc tcgagctgaa
    62041 tttgtgtctc ttcgcgctct ttgatttcaa ttttcagttg gccgaaggtt tcctgcagtt
    62101 ctgcttccgc tttttcacga acggcaattt cctgatttag ctgggcaata ttatctttta
    62161 actgaacgtt gaggctcaaa tcgcgctcgc gcatctcctc cagtttttgc accagccgtg
    62221 acagacgttg tcgtgactcc tccagttgct cgacgaccac cgatagaaag tagaccgccc
    62281 acggcgtaat cagcaaacca aagaagatag aacgaataac atcaatgctt tcgacctgac
    62341 catgcagcac catggttacc gccatttgta ccacaatggc aagaacgacg agggccagcg
    62401 ccagcaacat tgagaagcgc accagaccta acttcatcat caggtcaaca taatactgcg
    62461 ccagcagacg aatttgcttc attagggaat tccttcacga caacctgccc caataatacc
    62521 caattctgac agttacgtta taaattgtgt gagaaatgcg gaattcccct caacgaccta
    62581 ctccgttgga ggtagccacg gacgtcccag cgccgatccc tgtacgccat gttcattcag
    62641 atagcgatcc agctcgacca ttcccgtcca tcgattttcg caccacagcg gagcaagcag
    62701 cgtcgggcga cgggcgctgg cggaaatgcg gtggtagatc acttccggtg gcgtatggcg
    62761 aatcatttct ccggcagtga gcgtataatc ctccagttca ataccgttca aacgccccgc
    62821 ttcccaggct ttcgccataa tgctgccttt cacaatatgc agcggatgca gctttatgcc
    62881 gtccacgctg gtttcaacca ctcgctccag cgtttgcaaa cattcagcct gcccttcgcc
    62941 aggcaggccg acaattaagt gggaacacac cttcagcccg cgctgacgtg ccagctgggt
    63001 tgtacgctga taacaggcaa aatcatggcc gcggttgatg cgatgcagtg ttttgtcgtg
    63061 ggcggtttgt agccccagct ccagccacac ttcgtaaccc tggtccttat attcgcaaag
    63121 cagatccagc accgcatccg gcacgcagtc cgggcgggta ccaacacaca aaccgacaat
    63181 attggcctgg ctcaccgcct gctgatacat agaacgcagc acctgaactt ccgcaaaggt
    63241 gctggtatac gcctgaaagt aggccagata gcgtttagcg cggttaacta aattcgcctg
    63301 gtgcgccagt tgctcggcaa tggaacgatg ctgctgcgct tcatcggcaa acgaggcaac
    63361 attacagaat gtgcagccac cacgcccgat ggtaccgtca cggttagggc agctaaaacc
    63421 gccgtgcagc gtcagcttat gcaccttttg cccataacga cgggtgagat caccaccaaa
    63481 catattgact aatttctgta actgcataat ctgatagacc gcgccttgaa aagaggccaa
    63541 agcctgccat ttttagctca attcggcgat gacctggatc aatcgtcccg gcctgctttt
    63601 atcaactgca taatcaatca aaattaccga aatttcatgc acaatcacat aaatcacttt
    63661 tgcttatctt gtgtcagatt tttttatctc cttatggatt ttaggcaaaa acagtagcat
    63721 gaaacgtcat taccaattaa ggcagtataa aatgctggtt ttctcgtcag ttcaaggcag
    63781 gataagggtt aacacgcctt tatgacagtc aggaattgac tgtttctcta acgactctcc
    63841 ttttagcctt aaagataaaa tctattttaa tttcagctat ttaataaaga attttgcgct
    63901 aaagcacatt tctgtaccaa taagcttgcc atttgacctg tatcagcttt cccgataagt
    63961 tggaaatccg ctggaagctt tctggatgag cagcctgctc atcatattta tgcagtaatt
    64021 gagatcccct cttcaccgta ttaaccgatg cgaaaaggac aacaaggggg cgaatgcgag
    64081 gcgcgcgtat gacacgcaaa ccccgtcgcc acgctctttc tgtgcccgtg cgcagcggtt
    64141 cggaagtggg gttcccgcag agcctggggg aggttcacga tatgttgtac gataaatccc
    64201 ttgagaggga taactgtggt ttcggcctga tcgcccacat agaaggcgaa cctagccaca
    64261 aggtagtgcg tactgcaata cacgcactgg cccgcatgca gcaccgtggc gcgattctcg
    64321 ccgatggtaa aaccggcgac ggttgcggct tgctgttaca aaaaccggat cgcttttttc
    64381 gcatcgttgc gcaggagcgc ggctggcgtt tagcaaaaaa ctacgctgtc gggatgctct
    64441 tcctgaataa agatcctgaa ctcgccgctg ccgcacgccg catcgttgaa gaagaactgc
    64501 aacgcgaaac cttgtcgatt gtgggctggc gtgatgtccc cactaacgaa ggcgtgctgg
    64561 gtgaaatcgc cctctcctct ctgccacgca ttgagcaaat ttttgtgaac gccccggcag
    64621 gctggcgtcc acgcgatatg gagcgccgtc tgtttatcgc ccgccgccgc attgaaaagc
    64681 gtctcgaagc cgacaaagac ttctacgtct gtagcctgtc gaatctggtg aacatctata
    64741 aaggtctgtg tatgccggcg gatctgccgc gcttttatct ggatcttgcg gacctgcgtc
    64801 tggaatcggc catttgcctg ttccaccagc gcttctccac taacaccgta ccgcgctggc
    64861 cgctggcgca accgttccgc tatctggcgc ataacggtga aatcaacacc atcaccggta
    64921 accgccaatg ggcgcgtgcg cgtacctata aattccagac gccgctgatc cctgacctgc
    64981 acgacgccgc gccattcgtc aacgaaaccg gttctgactc cagttccatg gataacatgc
    65041 tggaactgct gctggcaggc gggatggata tcatccgcgc catgcgcctg ttagtgccac
    65101 ccgcctggca gaacaacccg gatatggacc cggaactgcg tgccttcttt gactttaact
    65161 ccatgcatat ggagccgtgg gatggtccgg cgggcatcgt gatgtccgat ggtcgcttcg
    65221 ctgcctgtaa cctcgaccgt aacggtctgc gtccggcacg ctacgtcatt accaaagaca
    65281 agctcatcac ctgcgcctct gaagtcggta tctgggatta ccagcctgac gaagtggtcg
    65341 aaaaaggccg cgtcgggcca ggcgaactga tggtgatcga cactcgcagt gggcgtattc
    65401 tgcactcggc agaaaccgat gacgatctga aaagccgcca cccttataaa gagtggatgg
    65461 agaaaaacgt ccgccgactg gtaccgtttg aagatctgcc cgatgaagaa gtgggtagcc
    65521 gcgaactgga cgacgacacg cttgccagct accagaaaca gtttaactac agcgcggaag
    65581 agctggactc cgtaattcgc gtactgggcg aaaacggtca ggaagcggtc ggttcgatgg
    65641 gcgatgatac cccattcgcc gtgctctcca gtcagccgcg cattatttac gactacttcc
    65701 gccagcagtt tgcccaggtg actaacccgc caatcgaccc gctgcgtgaa gcgcatgtta
    65761 tgtcgctcgc caccagtatc ggtcgtgaaa tgaacgtctt ttgcgaagca gagggccagg
    65821 cgcaccgttt aagctttaaa tcgccgattc tgctctactc cgatttcaaa cagctcacga
    65881 cgatgaaaga ggagcactac cgcgcagata cgctggatat cacctttgac gtcactaaaa
    65941 ccacgctcga agcgacagtc aaagagctat gcgacaaagc cgaaaaaatg gtacgtagcg
    66001 gcaccgtgtt gctggtgctc tccgaccgga atatcgctaa agatcgcctg ccggttccgg
    66061 ctccgatggc ggttggcgcg atccagaccc gtctggtcga tcaaagcctg cgttgcgatg
    66121 ccaacatcat cgtcgaaacc gccagcgccc gcgatccgca ccacttcgcc gtgttgctgg
    66181 gtttcggcgc gacggctatt tatccgtacc ttgcctatga aacgctgggc cgcctggtag
    66241 acacccacgc gattgccaaa gattatcgta ccgtgatgct caactaccgt aacggcatca
    66301 acaaaggctt gtacaaaatc atgtccaaaa tgggcatctc caccatcgcc tcttaccgct
    66361 gctcgaaact gtttgaagcg gtcggtctac acgatgatgt agtgggcctg tgcttccagg
    66421 gcgcggtcag ccgcattggt ggggcaagct ttgaagactt ccagcaggat ctgctgaatc
    66481 tgtcgaaacg tgcctggctg gcgcgcaagc cgattagcca gggcggcctg ctgaaatacg
    66541 tccacggcgg cgaataccac gcctacaacc cggacgtggt gcgcacgctg caacaggcgg
    66601 tacaaagcgg cgagtacagc gactatcagg aatacgcgaa gctggttaat gagcgtccgg
    66661 caaccacgct gcgcgatctg ctggcaatta cgccgggtga aaacgcggtc aacattgctg
    66721 atgttgaacc ggcaagcgaa ctgtttaaac gctttgatac cgccgcgatg tctatcggcg
    66781 cgttaagccc ggaagcccac gaggcgctgg cggaagcgat gaatagcatc ggcggtaact
    66841 cgaactccgg tgaaggcggc gaagacccgg cgcgctatgg cactaacaaa gtgtcgcgca
    66901 tcaagcaggt ggcttctggt cgcttcggcg ttactccggc gtatctggtc aatgccgacg
    66961 tcattcagat taaagtcgcc cagggcgcga agccgggcga aggtggtcag ttgccgggcg
    67021 acaaagtcac tccttacatc gccaaactgc gctattcggt gcccggagtg acgctgatct
    67081 ccccgccgcc gcaccacgat atctactcta tcgaagactt agcgcagctt attttcgacc
    67141 tcaagcaggt taacccgaaa gcgatgatct ccgttaagct ggtgtccgaa ccaggcgttg
    67201 gcaccatcgc gactggcgtg gcgaaagctt atgcggactt gatcaccatc gcgggctatg
    67261 acggcggcac cggcgcaagc ccgctgtcgt cagtgaaata tgcaggctgc ccgtgggaac
    67321 tcggtctggt ggaaacccaa caggcgctgg ttgccaacgg cttgcgtcat aaaattcgtt
    67381 tgcaggttga tggcggcctg aaaacgggtg tcgatatcat caaagcggcg attctcggcg
    67441 cagaaagctt cggcttcggc actggcccaa tggtggcgct cggctgtaaa tatctgcgta
    67501 tttgccacct gaacaactgc gccacgggtg tagcaactca ggatgacaaa ctgcgtaaga
    67561 atcactatca cggcctgccg ttcaaggtga ctaattactt tgagtttatc gcccgtgaaa
    67621 cccgcgagct gatggcacag ctgggagtaa cgcgtctggt ggatctgatt ggtcgcaccg
    67681 acctgctgaa agagctggac ggtttcaccg ccaaacagca gaaactggcg ctgtcgaagc
    67741 tgctggagac tgccgagcca catgcaggta agtcactcta ctgcactgaa aacaacccgc
    67801 cgtttgataa cggcctactg aacgcgcagt tgctgcaaca ggcgaaaccg tttgtcgatg
    67861 aacgccagag caaaaccttc tggttcgata tccgcaacac cgaccgttct gtcggcgcgt
    67921 cgctttcagg ctatatcgcc cagacgcacg gcgatcaggg gctggcagcc gatcctatca
    67981 aagcgtactt caacggcacc gcaggccaga gtttcggtgt gtggaacgcg ggcggcgtgg
    68041 aactgtacct gaccggggat gccaacgact atgtcggtaa aggcatggcg ggcggcttaa
    68101 tcgccattcg tcctccggtt ggttccgcct tccgcagcca tgaagcaagc attatcggca
    68161 acacctgcct gtatggcgcg accggtggtc gtctgtatgc cgcaggccgc gcgggtgaac
    68221 gtttcggcgt gcggaactcc ggtgctatca ccgtggtaga aggcattggc gacaacggct
    68281 gtgaatatat gacgggcggt attgtctgta ttctgggtaa aaccggcgtt aacttcggcg
    68341 cgggcatgac cggcggcttc gcttacgttc tcgatgaaag cggtgatttc cgcaaacgcg
    68401 ttaacccgga actggtcgaa gtcttaagcg tcgacgatct ggcgatccat gaagagcatc
    68461 tgcgcggtct tatcaccgag catgttcagc ataccggttc tcagcgcggt gaagagattc
    68521 tggcgaactg gtcaaccttc gccacgaaat tcgcgctggt taaaccgaag tccagtgatg
    68581 taaaagcact gctgggtcac cgtagtcgta gcgcagcaga gttgcgcgtg caggcgcagt
    68641 aaggggtagc aacaatgagt cagaatgttt atcaatttat cgacctgcag cgcgttgatc
    68701 cgccaaagaa accgctgaag atccgtaaaa ttgagtttgt tgaaatttac gagccgtttt
    68761 ccgaaggcca ggccaaagcg caggctgacc gctgtctgtc gtgcggcaac ccatactgcg
    68821 agtggaaatg cccggtacac aactacatcc caaactggct gaagctcgcc aacgaggggc
    68881 gtatttttga agcggcagaa ctgtcgcatc agaccaacac actgccggaa gtatgcggac
    68941 gtgtctgccc gcaagaccgt ctgtgcgaag gttcctgcac cctgaacgat gagttcggtg
    69001 cggtgaccat cggcaacatt gagcgttata tcaatgataa agcgttcgaa atgggctggc
    69061 gtccggatat gtccggcgtg aagcaaaccg gtaaaaaagt ggcgattatc ggtgcaggcc
    69121 cggcaggtct ggcgtgtgcg gatgtcctga cgcgtaacgg cgtaaaagcc gttgtcttcg
    69181 accgtcatcc agaaattggc gggctgctga ccttcggtat tccggccttc aagctggaaa
    69241 aagaggtaat gacgcgtcgc cgtgaaatct tcaccggcat gggtattgaa ttcaaactca
    69301 ataccgaagt gggccgcgac gtacagctgg acgatctgct gagtgattac gatgccgtgt
    69361 tccttggcgt tgggacttat cagtcaatgc gcggcgggct ggaaaacgaa gacgccgatg
    69421 gcgtgtacgc agcgctgccg ttccttatcg ccaacaccaa acagttaatg ggctttggtg
    69481 aaacccgcga cgaaccgttc gtcagcatgg aaggcaaacg cgtggtagta cttggcggtg
    69541 gcgacactgc gatggactgc gtgcgcacgt ctgtacgcca gggggcaaag cacgttacct
    69601 gtgcctatcg tcgtgatgaa gagaacatgc cgggttcccg ccgcgaagtg aaaaacgcgc
    69661 gggaagaagg cgtagagttc aaattcaacg tccagccact ggggattgaa gtgaacggta
    69721 acggcaaagt cagcggcgta aaaatggtac gtaccgaaat gggcgaaccg gatgccaaag
    69781 gccgtcgccg cgcggagatc gttgcaggtt ccgaacatat cgttccggca gatgcggtga
    69841 tcatggcgtt tggtttccgt ccacacaaca tggaatggct ggcaaaacac agcgtcgagc
    69901 tggattcaca aggccgcatc atcgctccgg aaggcaacga caacgctttc cagaccagca
    69961 acccgaaaat ctttgctggc ggcgatatcg tccgtggttc cgatctggtc gttaccgcca
    70021 tcgccgaagg tcgcaaagcg gcagacggca ttatgaactg gctggaagtt taagctctgt
    70081 cccagcgccg cctcctcaga aaagagtcgg cgctgaaatt ctcctttctt tctggaaatc
    70141 gcctcgcata atctgaagaa aaccaatgta atacgctcat ctggattgtt accggtagat
    70201 gcccggcgta tagctgattc cattattcaa cgcagcgcca tataaaggga cagataatgg
    70261 aatcgctctc agagagaacc tcaacaggct accagcaaat ccacaacggc attattcatc
    70321 tggtcgatag cgcccgaacg gaaacggtac gtagcgttaa cgcgctgatg accgcgacgt
    70381 actgggaaat tggccgacgg attgtcgaat ttgaacaagg tggtgaggcc agagcagctt
    70441 atggtgcaca gttgattgat cggctatcga aggatttaag tcgacggtat aaacgtgggg
    70501 tctctgcaaa aaacttacgc caaatgaggc ttttttacct cttttttcaa catgttgaaa
    70561 ttcgccagac agtgtctggc gaattaacac cattgggaat tccgcagaca ccatctgcag
    70621 aattcccatc ggcaaagatt tggcagacgc tgtctgccaa attgagaggc tgtttgatat
    70681 ttccacagct tttgtattgc atctcctatt ttcggtttgt atattcatgc aaataaacga
    70741 agg
//
LOCUS       DAZXUL010000003        67800 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZXUL010000003 DAZXUL010000000
VERSION     DAZXUL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67800
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.003"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..111
                     /locus_tag="ACH3L1_000152"
     CDS             <1..111
                     /locus_tag="ACH3L1_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFT4075080.1"
                     /translation="LRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            127..357
                     /locus_tag="ACH3L1_000153"
                     /pseudo
     CDS             127..357
                     /locus_tag="ACH3L1_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
     gene            752..1391
                     /locus_tag="ACH3L1_000154"
                     /pseudo
     CDS             752..1391
                     /locus_tag="ACH3L1_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1494..1817
                     /locus_tag="ACH3L1_000155"
     CDS             1494..1817
                     /locus_tag="ACH3L1_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HFT4075081.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1884..3341)
                     /gene="nuoN"
                     /locus_tag="ACH3L1_000156"
     CDS             complement(1884..3341)
                     /gene="nuoN"
                     /locus_tag="ACH3L1_000156"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFT4075082.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3348..4877)
                     /gene="nuoM"
                     /locus_tag="ACH3L1_000157"
     CDS             complement(3348..4877)
                     /gene="nuoM"
                     /locus_tag="ACH3L1_000157"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFT4075083.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5041..6882)
                     /gene="nuoL"
                     /locus_tag="ACH3L1_000158"
     CDS             complement(5041..6882)
                     /gene="nuoL"
                     /locus_tag="ACH3L1_000158"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFT4075084.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6879..7181)
                     /gene="nuoK"
                     /locus_tag="ACH3L1_000159"
     CDS             complement(6879..7181)
                     /gene="nuoK"
                     /locus_tag="ACH3L1_000159"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFT4075085.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7178..7732)
                     /gene="nuoJ"
                     /locus_tag="ACH3L1_000160"
     CDS             complement(7178..7732)
                     /gene="nuoJ"
                     /locus_tag="ACH3L1_000160"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFT4075086.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7744..8286)
                     /gene="nuoI"
                     /locus_tag="ACH3L1_000161"
     CDS             complement(7744..8286)
                     /gene="nuoI"
                     /locus_tag="ACH3L1_000161"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFT4075087.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8301..9278)
                     /gene="nuoH"
                     /locus_tag="ACH3L1_000162"
     CDS             complement(8301..9278)
                     /gene="nuoH"
                     /locus_tag="ACH3L1_000162"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFT4075088.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9275..12001)
                     /gene="nuoG"
                     /locus_tag="ACH3L1_000163"
     CDS             complement(9275..12001)
                     /gene="nuoG"
                     /locus_tag="ACH3L1_000163"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFT4075089.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCISCYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12054..13391)
                     /gene="nuoF"
                     /locus_tag="ACH3L1_000164"
     CDS             complement(12054..13391)
                     /gene="nuoF"
                     /locus_tag="ACH3L1_000164"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFT4075090.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13388..13888)
                     /gene="nuoE"
                     /locus_tag="ACH3L1_000165"
     CDS             complement(13388..13888)
                     /gene="nuoE"
                     /locus_tag="ACH3L1_000165"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFT4075091.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13891..15693)
                     /gene="nuoC"
                     /locus_tag="ACH3L1_000166"
     CDS             complement(13891..15693)
                     /gene="nuoC"
                     /locus_tag="ACH3L1_000166"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFT4075092.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15787..16449)
                     /gene="nuoB"
                     /locus_tag="ACH3L1_000167"
     CDS             complement(15787..16449)
                     /gene="nuoB"
                     /locus_tag="ACH3L1_000167"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFT4075093.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16465..16908)
                     /gene="nuoA"
                     /locus_tag="ACH3L1_000168"
     CDS             complement(16465..16908)
                     /gene="nuoA"
                     /locus_tag="ACH3L1_000168"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFT4075094.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17539..18477)
                     /gene="lrhA"
                     /locus_tag="ACH3L1_000169"
     CDS             complement(17539..18477)
                     /gene="lrhA"
                     /locus_tag="ACH3L1_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFT4075095.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19396..20613
                     /gene="alaA"
                     /locus_tag="ACH3L1_000170"
     CDS             19396..20613
                     /gene="alaA"
                     /locus_tag="ACH3L1_000170"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFT4075096.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20697..21296
                     /gene="yfbR"
                     /locus_tag="ACH3L1_000171"
     CDS             20697..21296
                     /gene="yfbR"
                     /locus_tag="ACH3L1_000171"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFT4075097.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRFQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21355..23187)
                     /locus_tag="ACH3L1_000172"
     CDS             complement(21355..23187)
                     /locus_tag="ACH3L1_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFT4075098.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23274..23924)
                     /gene="hxpA"
                     /locus_tag="ACH3L1_000173"
     CDS             complement(23274..23924)
                     /gene="hxpA"
                     /locus_tag="ACH3L1_000173"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFT4075099.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23935..24429)
                     /locus_tag="ACH3L1_000174"
     CDS             complement(23935..24429)
                     /locus_tag="ACH3L1_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFT4075100.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24512..24967)
                     /gene="yfbV"
                     /locus_tag="ACH3L1_000175"
     CDS             complement(24512..24967)
                     /gene="yfbV"
                     /locus_tag="ACH3L1_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFT4075101.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25305..26507
                     /gene="ackA"
                     /locus_tag="ACH3L1_000176"
     CDS             25305..26507
                     /gene="ackA"
                     /locus_tag="ACH3L1_000176"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFT4075102.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26582..28726
                     /gene="pta"
                     /locus_tag="ACH3L1_000177"
     CDS             26582..28726
                     /gene="pta"
                     /locus_tag="ACH3L1_000177"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFT4075103.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28916..30436
                     /gene="yfcC"
                     /locus_tag="ACH3L1_000178"
     CDS             28916..30436
                     /gene="yfcC"
                     /locus_tag="ACH3L1_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFT4075104.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30469..31011)
                     /gene="yfcD"
                     /locus_tag="ACH3L1_000179"
     CDS             complement(30469..31011)
                     /gene="yfcD"
                     /locus_tag="ACH3L1_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFT4075105.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31069..31620)
                     /gene="yfcE"
                     /locus_tag="ACH3L1_000180"
     CDS             complement(31069..31620)
                     /gene="yfcE"
                     /locus_tag="ACH3L1_000180"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFT4075106.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31676..32320)
                     /gene="yfcF"
                     /locus_tag="ACH3L1_000181"
     CDS             complement(31676..32320)
                     /gene="yfcF"
                     /locus_tag="ACH3L1_000181"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFT4075107.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32456..33103
                     /gene="yfcG"
                     /locus_tag="ACH3L1_000182"
     CDS             32456..33103
                     /gene="yfcG"
                     /locus_tag="ACH3L1_000182"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFT4075108.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33160..33522
                     /gene="folX"
                     /locus_tag="ACH3L1_000183"
     CDS             33160..33522
                     /gene="folX"
                     /locus_tag="ACH3L1_000183"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFT4075109.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33543..34436
                     /locus_tag="ACH3L1_000184"
     CDS             33543..34436
                     /locus_tag="ACH3L1_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFT4075110.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34484..35374)
                     /gene="rpnB"
                     /locus_tag="ACH3L1_000185"
     CDS             complement(34484..35374)
                     /gene="rpnB"
                     /locus_tag="ACH3L1_000185"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFT4075111.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35571..36344)
                     /gene="hisP"
                     /locus_tag="ACH3L1_000186"
     CDS             complement(35571..36344)
                     /gene="hisP"
                     /locus_tag="ACH3L1_000186"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFT4075112.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36352..37068)
                     /locus_tag="ACH3L1_000187"
     CDS             complement(36352..37068)
                     /locus_tag="ACH3L1_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4075113.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37065..37751)
                     /gene="hisQ"
                     /locus_tag="ACH3L1_000188"
     CDS             complement(37065..37751)
                     /gene="hisQ"
                     /locus_tag="ACH3L1_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFT4075114.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37841..38623)
                     /gene="hisJ"
                     /locus_tag="ACH3L1_000189"
     CDS             complement(37841..38623)
                     /gene="hisJ"
                     /locus_tag="ACH3L1_000189"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFT4075115.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38844..39626)
                     /gene="argT"
                     /locus_tag="ACH3L1_000190"
     CDS             complement(38844..39626)
                     /gene="argT"
                     /locus_tag="ACH3L1_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFT4075116.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39892..40461)
                     /gene="ubiX"
                     /locus_tag="ACH3L1_000191"
     CDS             complement(39892..40461)
                     /gene="ubiX"
                     /locus_tag="ACH3L1_000191"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFT4075117.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40556..42073)
                     /gene="purF"
                     /locus_tag="ACH3L1_000192"
     CDS             complement(40556..42073)
                     /gene="purF"
                     /locus_tag="ACH3L1_000192"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFT4075118.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(42110..42598)
                     /gene="cvpA"
                     /locus_tag="ACH3L1_000193"
     CDS             complement(42110..42598)
                     /gene="cvpA"
                     /locus_tag="ACH3L1_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFT4075119.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42857..43519)
                     /gene="dedD"
                     /locus_tag="ACH3L1_000194"
     CDS             complement(42857..43519)
                     /gene="dedD"
                     /locus_tag="ACH3L1_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFT4075120.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43509..44777)
                     /gene="folC"
                     /locus_tag="ACH3L1_000195"
     CDS             complement(43509..44777)
                     /gene="folC"
                     /locus_tag="ACH3L1_000195"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFT4075121.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44847..45761)
                     /gene="accD"
                     /locus_tag="ACH3L1_000196"
     CDS             complement(44847..45761)
                     /gene="accD"
                     /locus_tag="ACH3L1_000196"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFT4075122.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45917..46576)
                     /locus_tag="ACH3L1_000197"
     CDS             complement(45917..46576)
                     /locus_tag="ACH3L1_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFT4075123.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46659..47471)
                     /gene="truA"
                     /locus_tag="ACH3L1_000198"
     CDS             complement(46659..47471)
                     /gene="truA"
                     /locus_tag="ACH3L1_000198"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFT4075124.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47471..48484)
                     /locus_tag="ACH3L1_000199"
     CDS             complement(47471..48484)
                     /locus_tag="ACH3L1_000199"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFT4075125.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48550..49686)
                     /gene="pdxB"
                     /locus_tag="ACH3L1_000200"
     CDS             complement(48550..49686)
                     /gene="pdxB"
                     /locus_tag="ACH3L1_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFT4075126.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49785..50780
                     /gene="flk"
                     /locus_tag="ACH3L1_000201"
     CDS             49785..50780
                     /gene="flk"
                     /locus_tag="ACH3L1_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFT4075127.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50777..51955)
                     /locus_tag="ACH3L1_000202"
     CDS             complement(50777..51955)
                     /locus_tag="ACH3L1_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4075128.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52210..53451)
                     /gene="fabB"
                     /locus_tag="ACH3L1_000203"
     CDS             complement(52210..53451)
                     /gene="fabB"
                     /locus_tag="ACH3L1_000203"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFT4075129.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53610..55616
                     /gene="mnmC"
                     /locus_tag="ACH3L1_000204"
     CDS             53610..55616
                     /gene="mnmC"
                     /locus_tag="ACH3L1_000204"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFT4075130.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55737..56015)
                     /locus_tag="ACH3L1_000205"
     CDS             complement(55737..56015)
                     /locus_tag="ACH3L1_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFT4075131.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(56049..56597)
                     /gene="epmC"
                     /locus_tag="ACH3L1_000206"
     CDS             complement(56049..56597)
                     /gene="epmC"
                     /locus_tag="ACH3L1_000206"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFT4075132.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56597..57406)
                     /locus_tag="ACH3L1_000207"
     CDS             complement(56597..57406)
                     /locus_tag="ACH3L1_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFT4075133.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57406..58230)
                     /gene="mepA"
                     /locus_tag="ACH3L1_000208"
     CDS             complement(57406..58230)
                     /gene="mepA"
                     /locus_tag="ACH3L1_000208"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFT4075134.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58234..59319)
                     /gene="aroC"
                     /locus_tag="ACH3L1_000209"
     CDS             complement(58234..59319)
                     /gene="aroC"
                     /locus_tag="ACH3L1_000209"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFT4075135.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59354..60286)
                     /gene="prmB"
                     /locus_tag="ACH3L1_000210"
     CDS             complement(59354..60286)
                     /gene="prmB"
                     /locus_tag="ACH3L1_000210"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFT4075136.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60452..61003
                     /gene="smrB"
                     /locus_tag="ACH3L1_000211"
     CDS             60452..61003
                     /gene="smrB"
                     /locus_tag="ACH3L1_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFT4075137.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(61107..61997)
                     /locus_tag="ACH3L1_000212"
     CDS             complement(61107..61997)
                     /locus_tag="ACH3L1_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFT4075138.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61984..62508)
                     /locus_tag="ACH3L1_000213"
     CDS             complement(61984..62508)
                     /locus_tag="ACH3L1_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4075139.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62505..62975)
                     /locus_tag="ACH3L1_000214"
     CDS             complement(62505..62975)
                     /locus_tag="ACH3L1_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4075140.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62972..63520)
                     /locus_tag="ACH3L1_000215"
     CDS             complement(62972..63520)
                     /locus_tag="ACH3L1_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4075141.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63525..64247)
                     /locus_tag="ACH3L1_000216"
                     /pseudo
     CDS             complement(63525..64247)
                     /locus_tag="ACH3L1_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64267..66907)
                     /gene="yfcU"
                     /locus_tag="ACH3L1_000217"
                     /pseudo
     CDS             complement(64267..66907)
                     /gene="yfcU"
                     /locus_tag="ACH3L1_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66989..67552)
                     /locus_tag="ACH3L1_000218"
     CDS             complement(66989..67552)
                     /locus_tag="ACH3L1_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4075142.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16963 a        18308 c        16817 g        15712 t
ORIGIN      
        1 ctgagacaac atctggcaag gctgggacgg aagtcactgt cgttctcaaa atcggtggag
       61 ctgcatgaca aggtcatcgg gcattatctg aacataaaac actatcagta agttggagtc
      121 attaccaggg aaagcaattc tgacagcgcc ccctatacgt tattaaaaaa ctatgctgac
      181 agatttaaaa aatattcagc agaagagcag tacgaaattg attttcaaca tcgactggta
      241 aacagaaatt gttatttaga taaatatggc gatgcacgaa tcaatgcttc ttatacacaa
      301 ttagaaataa aacactcaca acccaaaaat agagcatccg gcaaaagagt gagttaacat
      361 cattgttaat caattcgacc agtcagtaaa tatgctgact ggtcgaacat aatttgctaa
      421 aaagtcatca ctgactgatg tcagtcccac catctgccag ttcaatctac gggtatttcc
      481 agcatatcgc ggcacgcgat tgctggtctg ttgatgtagt ggattgtagt tgctgacttt
      541 tcttaccctc agatttctgc cccacttgtc aattcaatct gccaataaat aacataatta
      601 tatttttatt cctcacagaa attccttttt aaaaatagcg aacccactgt aaaaaacact
      661 ttttgtatac ctctaccaag cgtaataaaa taaacactca taattcatta taaataataa
      721 tgaaatgacg ccttttattg ataatttaca tatgaaaaaa ttttcctttt gttatattat
      781 ttctgttttc actccctctg attattttta ccaaccctgg gtcactgcat tatcgccaac
      841 gccccgacac gtaagtcctg agcaattaga aaaaacagtt cgttatctta cacaaactgt
      901 gcatccacgt aatgccggca atattgataa tctgaatagg tccgcagagt atataaaaga
      961 gttctttgtc agtagcggtg ccagagttac cgcgcagggc gtccccgatt ggctattacg
     1021 actctgcgcc tggaagccag aattaccctt acccggcaat gtcctggctt tatcccgatc
     1081 ggggagactt tattgctgtg gtcggcagaa tgcaggatat caacgccgtt cgtcaggtaa
     1141 aagcggcatt gttgtcatcc cgggatttat ctgtttattc tatgaatgcc ccagggttta
     1201 ttcccggtat tgatttttct gatcacctca attattggca acacgatatt cccgccgtaa
     1261 tgattactga caccgctttt tatcgtaata aacaatacca cttgcccggt gataccgcag
     1321 acagattgaa ttatcagaaa atggctcagg tggtagatgg tgttataacc ttgttataca
     1381 acagtaaata ataaaaattt ctcgaggcgg gtccagaaac agcgacatgt acattttatt
     1441 ttccctcgtc tgttttaaga tgcgaaacat aaaattcaat ggaggaaaaa taaatgggaa
     1501 tgattggcta ttttgcagaa attgactcag aaaagattaa ccaactgctt gagtctactg
     1561 aaaaaccttt gatggataat atacatgaca cgctttcagg attacgccga ctggaatcac
     1621 tggatcgaag tgagctgcgt aaacagtttt ccattaagcg actcaatgaa atggagattt
     1681 atccgggagt gaccttttca gaagagttag aggggcaact ctttgcctcg atcatgcttg
     1741 atatggaaaa gctgattagc gcgtatcgac gtatgctgcg acaagggaat catgcgctga
     1801 cggttattgt tgggtgatgg tgtcagagcg ccaaaaaaaa ccgccgaaca tgtcggcggc
     1861 tttctgactt acaaactaac agtttacatc agcggcattg ctaaccgcac aatactaatc
     1921 agcggttgtg gccatacacc cagcaccagt accaacagtg cagaaatcag caccacgata
     1981 ccgcccgcgc tgtactgcca gtttgacggt gcatcgcgac ccggttgttc cggtgcgtgc
     2041 aggtacaggc tcaccgccac gcgcaggtag tagtagaggc cgattgccga accgacaacc
     2101 acggcaccca ccagccacca caagtgtgcc tggacaccga ccgccagcac gtagaactta
     2161 ccgataaagc ccagcgtcat cgggataccg gccagagaca gcatcatcac cgtcatcact
     2221 gccgcgagga tcggacgatg ccagaacaga ccgcggtaag agaacaggga atcagcatcc
     2281 gggccacgat acgggctgga catcaggctg accacgccga acgcgccgag gctgctgaac
     2341 agataaccgg ccaggtaaac ccctaccgct tccatcgaca tctcgccggt ttgcagcgca
     2401 atcagcgcca ccagcagata gccgaggtga gagatagatg agtaaccgag cagacgtttg
     2461 atgttggtct ggctcaacgc catcaggtta ccgaagatga tggaggcaaa ggcgataatc
     2521 gccagtacta cgcgaatcgc ttcgctgtca cccaccggtg cgtagaggaa cagacgcatc
     2581 accacaccga agatagcgat tttgctcgcc gtcgccagga aagtggaaac cggcgcaggc
     2641 gcgccctggt atacgtctgg cgtccacagg tggaacggca ccagagagag tttgaagccg
     2701 aggccaacaa tcatcatgcc gaaacctgcc agcaacagcg gctcgttgag cataccgtcg
     2761 ccgaggtttt tacccagcgc gacaaacgac aggtcgccag actgcgcata caccagcgcc
     2821 ataccgaaca gcaggaaaga agacgctgcg gcagaaagga tggtgtattt gatactggct
     2881 tccagcgaac gtttctggcg gaaagcgtaa ccgaccaggc caaacagcgg caaagagatc
     2941 agttcaatac cgaggaacag agacgccaga tggtttgcat tcgccagcag gatcccgccc
     3001 agcgcggcaa ttaacaccaa caggtagaac tcatccttgt tgtcgttata gccttcaagc
     3061 cacgggtagg cgaaagtaca ggtggcgagg ctcgccaaca ataccagccc ggtgtaaagc
     3121 atggcgaaac catcaacgcg catcagcggc gtaacgtcca tagcgcccgc ctggccaaca
     3181 aaccagagcg aaaccagcgc cgcgttaagc ccaataaccg agagcgtagc gttgaggaaa
     3241 tgattgcgtc gccacgcaat ggagagcatc acaaccacca ccgtcaagcc gacgatcagc
     3301 aacggtagca gtgcgatcag gttttgtgga gttattgtca tggcgattta cggccttgta
     3361 gtagtaacgg aattaacaaa ccactgctgg atattgccaa tcgcggagtg cgaggtatcc
     3421 agaatcggct gcggatagaa gcccagcagt accagcagca ccaccagcaa caggatcata
     3481 aacagctcac gcagcgacat ccctggcagt tcctggctgg caatctggct tttcgcttta
     3541 ccgaagtaag cgcgatgcaa catcgccagc gaataaacag atgcaaagac cagcccaaag
     3601 gtagagataa cggtaatcac cgggacaacc tggaagctgc cgaacagaat cataaattcg
     3661 ccgacgaagt taccggtgcc aggcatccca agcgttgcca ccgcaaagaa cagcgacagt
     3721 gctggcagcc atttcatctt gctccacaga ccgcccatca tgcgcatgtc gcgggtatgg
     3781 atacgttcat aaagctgacc gcagagaata aacagacccg ccgccgacaa gccgtgcgca
     3841 atcatctgga ttaccgcgcc ctggtaggcc aactggctgc cggtgtagat tgcaatcagc
     3901 acgaagccca tgtgggaaac cgaggtgtag gcgatcagac gtttgatatc ggtctgagcg
     3961 aaggtcatcc acgcaccgta gaagatgccg ataacaccca gccacatagc gattggcgcg
     4021 aactctgccg acgcgttcgg gaacagcggc agggagaaac gcaacaaacc gtaagcggcg
     4081 gttttcagca agatccccgc gaggtccacg gaaccggcgg tcggagcctg ggagtgcgca
     4141 tccggcagcc agccatgcag cggaaccacc ggcattttga ctgcgaaggc gatgaagaag
     4201 cccagcatca acagatattc cacaccactg gacattggcg tattcagcag ctcttcatag
     4261 ttgaaggtcc agacgccggt cgcattgtag tgaacaaaaa ccagcgccag gatggcgatc
     4321 aacatcacca gaccactcgc ctgggtgtaa atgaagaact tggttgccgc cgtgatacgc
     4381 gttttaccgt cagaggcttt atgcccccac agtgcgatca ggaagtacat cggcaccagc
     4441 atcatttccc agaagaagaa gaacaggaac atgtcgatgg caaggaacac gccgataacg
     4501 ccgcccagga tccacatcag gttgaggtgg aagaagccct gatatttttc gatctctttc
     4561 cacgaacata gtaccgccag cacaccgagc agaccggtca gcacgaccat cagcagcgac
     4621 agaccgtcaa tggcgagatg aatagagata ccaaaacgcg ggatccacgg catgtcgaat
     4681 tcagactgcc actgcggaat tccggcggat tgcgtcagtg aataaccgcc ctgcaaccac
     4741 agttgcagcg acagcgccag cgtcaatccc atggtgatca gcgcgatcca gcgtggcacc
     4801 ttgacgccaa agcgttcggt ctgccagcac aggaagccgc caataaaggg aattaatatt
     4861 agccagggta gtaacatggc gatctttatt ccttgtaaaa gtcccgtcag gaccggattt
     4921 tcaacgaatt ctcacgacag aaaattctct tcggactggg ggtagtgtcg gatgcgccgc
     4981 gaaggcagct tatccgacct acgttcttat atgcccaggg gcaatcccac aatccttaac
     5041 tcaacgcagt accatcaaca gtgccagcac cacgaccgca ccgatgctca tggatgccac
     5101 ataccagcgc agataaccgt tctcgcttaa cagcagacct ttacctgcaa agcgggaaag
     5161 aaccgccggg atgttcatca ttgagttcag cggatcgcgt ttcagcaacc aggcaatacc
     5221 caggaacggc ttgacgaaca ctttgtcgta cagccagtca aagccccaag cgttgtacca
     5281 ccaggtactc agcaggcggc ccggcgcact gttggcgatg gaggtcacca gagaacgttt
     5341 acccagccac agccaggctg ccagcagaat gccgaccacc gcgaccacgc cagaggtaat
     5401 ttccagggtc aacatgctgc cgtgcgccag ttccgtcgtt tgcggaagca cgccctgcag
     5461 cggcggtaca atcagtgcgc caacgaaggt ggaaaggatc agcagcacaa tcagcggcag
     5521 gctgtgagtt acccctttca cggcgtgagc gtgaatttgt tcttttccgt ggaagacgat
     5581 gaaaatcata cggaaggtgt agagcgaggt cataaacgca ccgaccagac ctgccaccat
     5641 cagattgata tgaccattcg ccatcgcacc cgcgaggatc tcatccttac tgaagaagcc
     5701 cgcagtgacc agcggcagtg ccgacagtgc tgcgccgccc accaggaagc agagataaac
     5761 cagcggaata gatttacgca gaccgcccat cttgaagatg ttctgttcgt gatggcaggc
     5821 cagaatgacg gaaccggatg ccaggaacag cagcgcttta aagaacgcgt gagtcatcaa
     5881 gtggaaaatc gccgcatccc atgcctgcac gccaagcgcg aggaacatgt agccaatctg
     5941 gctcatggta gagtaagcga gaacacgttt gatgtcggtc tgtaccagcg cggcaaaacc
     6001 ggccagcagc agcgtaaccg ccccgacaat acccaccaga tgcagaactt ccggcgtcat
     6061 caggaacagg ccgtgggtac gggcgatcag gtagacaccc gcggttacca tcgttgcggc
     6121 gtggatcagc gcggagacag gcgtcgggcc cgccatcgcg tcagcaagcc atgtctgcaa
     6181 cggcaactgc gcagatttac cgaccgcacc gcccagcagc atcagcgtcg cccacatcag
     6241 catgttattg ccgtcagcaa agtgcgctgg tgccagttcc accatatcgc ggaagttcag
     6301 ggtgcccagt tcgttgtaaa gaatgaacag tgcgaaagcg aggaaaacgt cacccacacg
     6361 ggtcacgacg aacgctttca ttgctgctgc gccattcttc ggatcggtgt aatagaaacc
     6421 gatcagcaga taggagcaca ggcccacgcc ttcccagccg aggtacatca gcagcaggtt
     6481 atcggcgaga accagaacta ccatgctggc gatgaacagg ttggtgtagg cgaagaagcg
     6541 agagtagccc tcttcaccgc gcatgtacca ggaggcgtac atgtgaataa ggaaacccac
     6601 accagtgacc accgagagca tggtcagcga caggccgtcc agcaccaggt taaaaccgat
     6661 gttaaagtcg cctaccgaca tccacgtcca cagcggctgg ctgtatgcct gctcgccgtt
     6721 agcgaagaaa tcaacgccga taaaggcggt taccagcgcc gccaggccca cagagccaac
     6781 gccgacgatc gccgaaacgt tttcagacca gcgcccacgg gagaacgcca gcaggacgaa
     6841 gccaatcaat ggcaaaataa tggttaaggc aagcatgttc atccgcgcat ctcacttact
     6901 gaatcgatgt tcaggttctg gcgacgacgg tgaagttgca gcagcagcgc aaggccgata
     6961 ctcgcttctg ccgccgcgag gctgatggcg agaatgtaca tcacctgacc gtcggtctga
     7021 ccccagtagc ttccggcgac cacaaaggcc agcgcggagg cgttaatcat gatttccaga
     7081 ccaatcagca taaacagcag attgcgacgg ataaccagac cggttaagcc aagaacgaat
     7141 aagattgccg cgaggatcag tccatgttgt aaggggatca tgcgtgctcc tccgtttttc
     7201 ttttcgcgct gtcgtcttta cgattgctca gcacttcacc cgcacgctct tcacgaccga
     7261 cgtggaaggc cacaaccaga cccgcgagca gcagcataga agccagttcc accgccagta
     7321 cgtaaggccc gaacagcgta atacccactg ctttagcact gattggcgta ccgtcgatac
     7381 cctgatcgtt aacaccgagg atggcgtaaa caatcaccac cagcatgatg gccgacaaaa
     7441 ttgccgggcc aatccacacc tgcggtttca gccactggcg ttcctgttcg atttctgaac
     7501 cgcccaggtt gagcatcatc accacgaaca cgaacagcac cataatggca cccgcgtaga
     7561 cgataatttc cagcgcaccg gcgaagtaag cgcctagtga gaagaacacc ccggaaatcg
     7621 ccagcagcga aataatcagg tacagcagtg cgtgtaccgg attggtatgg gtgatcactc
     7681 gcaaggtcgc aagtatggct atcaggccac agatataaaa agcgaactcc attgcccctc
     7741 tccttacggt aacaggctct tgacgtcgat aggcttggct tcgttctctg cttcgccctt
     7801 atctttgccg tcgattgcca tacctgccat ccggtagaag ttatattccg ggtatttgcc
     7861 cggaccggag atcagcagat cctctttctc gtaaaccaga tcctggcgct tgtattcccc
     7921 catttcgaaa tccggggtta actgaatcgc cgtggtcgga caggcttctt cgcacagacc
     7981 acagaaaatg cagcgtgaga agttaatgcg gaaaaattcc ggataccagc gaccgtcttt
     8041 ggtttctgct ttttgcagcg agatacagcc gaccgggcag gctaccgcac agaggttaca
     8101 ggctacgcaa cgctcttcgc cgtccgggtc gcgggtcaga acgatacgac cacgataacg
     8161 gggcggcaga tagaccggct cttccgggta cattcgcgtt tcgcgtttgg cgaacgcgtg
     8221 caggccgatc atccagatac tacgaacctg ggtgccgaaa cctactaaca attcttttaa
     8281 ggtcatggtc ttattgcccc ttattgcgcc tgccagagaa tgacagccgc cgttaccagc
     8341 aagttgatca gcgtcagcgg caggcagatt ttccagccga aggacattac ccggtcataa
     8401 cgcggacgcg gtaacgacgc acgaatcaaa atgaacatca tcataaagaa cgcggttttc
     8461 agcgcgaacc agatgaatgg cggtaacaac gggccttgcc agccaccgaa gaacagcgtc
     8521 accatcaatg cagagatggt cacaatcccg atgtactcac ccacgaagaa cagaccgaac
     8581 ttcataccgg aatattcaat gtggtaacca tccgccagtt cctgctcggc ttccggctgg
     8641 tcaaacgggt gacggtgaca caccgccacg cccgcgatgg caaaggtaat aaaaccaaag
     8701 aattgcggga tgacgttcca cacatgcgcc tggctgttga cgatgtcggt catgttgaat
     8761 gaaccggcct gcgccaccac gcccatcagg gaaagcccga ggaacacttc gtagctcagg
     8821 gtctgcgcag aagcacgcat cgcacccagc agcgagtatt tgttgttact tgaccagccc
     8881 gcaaacagca ccgcgtaaac cgccagacct gccatcatca ggaagaacaa aatcccgatg
     8941 ttcaggtcgg caaccaccca acccggactg actggcacaa ttgcaaaggc cagcagcagc
     9001 gaggtaaagg caatcatcgg tgccagggta aagatgacgc gatccgagaa tttcgggatc
     9061 cagtcttctt taaagaacat tttgatcatg tccgcaacca gctggagcga accgccccag
     9121 ccaacacggt taggtccata acggttctgg aacagaccca gcaggcgacg ttcgccaaag
     9181 ctcatgaatg ccccacaggt gacaaccacc agcaggatca ccaccgcttt gaggatggtc
     9241 agcaggatct caatcagttc cggtgatatc caactcattg ttgtgcctcc ttgagatcct
     9301 caagatgcgc gccagccagc accggagcaa tgccggacat acccatcggc aagcccacct
     9361 gccctgccgt cagtccttcg gcgatttcaa ccggcagcgt gaccgtgttg ccatcgtaac
     9421 taaaggagac gcgtgtacct gcgttcacac ccaacttcgc ggcatccgct gggttgagtt
     9481 tgatgtacgg ctgcggcata cggctctgga agaccggagc acgctgtgac aattcatcgc
     9541 tgccaaacag gtggtagtac ggcgcgatac gccatttccc gtcctgcggc tggaagcgtg
     9601 ccggtacgct ggtgaagtaa tccagaccgt tttcgctggt ttcaaacaga cgcacgcccg
     9661 gatcgccaaa gcgcagtttg ccgcccactt cgtcctggaa tttgttccac gcctgcgggg
     9721 agttccagcc cggcgcccag gcaaacggca cttgcgaacg gtgcgcggtc ggctggttgt
     9781 taccttccat cgagaaggtg aacatggtgt caatatcctg cggctgacgc ggctcatgaa
     9841 cgctgatatt ggcgcgcatg gcggtacgac cgctgtaacg gtgcggttca cgggccagtt
     9901 tctgcccacg aatacggaat gtcgcatccg gcgcagcatc tttgataccc gccagttctg
     9961 ggattttcgc cacaacagcg tcaattacat ggtcgagctg cgtccagtcc acttcacggc
    10021 tcagcaaggt gctgtgcagc gagtgcagcc agcgccagct ttccagcatg acagttttgc
    10081 tgtcgtaata agccggatcg taaacctgga agaaacgttg ggcgcgacct tcattattaa
    10141 ttaccgtacc gtcgctttca gcaaagctgg cagaggaaag caccagatgg gcgttttcca
    10201 tgatcgctgt gcgttgatga tcaaccacca tcaccagcgg cgctttagcc agcgcagcat
    10261 tcacgcgggt agcagaagcg taacgatgca gatcgttttc caacaccacc accgcgtcgg
    10321 cgcgtccggt ttccagttcg gttaacgctt cttcaagcga accgccaccc ataatgccca
    10381 gccccatgct gttgacggaa cgggcaatca tggtgatacc aacgtcagcg ccgcgacctt
    10441 tcagggcttt cgcgacgtta gccgcagcct gaatcacctc tatgctaccg gcgttcgtcc
    10501 cggagataat caacggtttc ttcgcacctg ccagtgcctg cacgatgacg tcgattttgt
    10561 tttgcagctc aggttcgata ccgtcaaccg ctggtgcgga gttatccagc gcatgggcga
    10621 tggcaaaacc taaacgcgcc tgatcttcaa ccggtgcgcg gtaagtccac gccgcgatat
    10681 catccagacg agtgtcatca acgttggtaa caaacagcgg atgcttcgca cgttgaccga
    10741 tgttgaggat tgccgcaatc tgccagtcag ccactttctg tgctgcagcc atttcgcgcg
    10801 ctttaccttt caccgcctga cgcactgcca gtgcgacgcg cgcgccggtc tgggtaacgt
    10861 cttcgcccag caccagtacc gcatcgtaag attcgatttc gcgcagagcc ggagtataaa
    10921 tgccgccttc acgcagcact ttcagcgcca gttgcagacg ttcctgctca ccgtgagcga
    10981 taccggtgta gaagttttct tcgcccacca gttcacgcag cgcaaagttg ctttccacgc
    11041 tggcacgcgg agaaccaata ccgatcactt tcttcgactg acgcagaata tctgccgcgc
    11101 cctgcattgc ctgttcggcg ttgagggtaa tgaaatcatc gccacgacgc tgtactggct
    11161 gacgcggacg atccttcagg ttgacgtaac cgtaaccgaa acgaccacgg tcgcagagga
    11221 agtagtggtt taccgtaccg ttgtaacggt tttcgatacg acgcagttcg ccgtaacgtt
    11281 caccggggct gatgttacag ccgatggaac attgctggca gatgctcggc gcaaactgca
    11341 tatcccattt acggttgtaa cgctcggagt gcgttttgtc ggtaaatacg ccggtcgggc
    11401 aaatttcgac caggttaccg gaaaattcgc tttccagcgt gccgtcttcc gggcgaccga
    11461 agtagacgtt gtcgtgcgcg ccataaacgc ccagatctgt accgtcagcg taatctttgt
    11521 agtaacgcac acagcggtag caggagatgc agcggttcat ttcgtgagag atgaatggcc
    11581 ccaaatcctg attacggtgg gtacgtttgg tgaaacggta gcgacggaag ctgtgtccgg
    11641 tcatcacggt catatcctga agatggcagt taccgccttc ttcacatacc ggacagtcgt
    11701 gcgggtggtt ggtcattaac cactcgacca cactttcacg gaactgtttc gcttcttcgt
    11761 cgtcaatgga aataaaggtg ccatcggaag ccggtgtcat acaggacatc accaggcgac
    11821 cacgcgtgtc ttccgcgttt tggtattgct tcaccgcaca ctggcggcaa gcaccgacgc
    11881 ttcccagcgc cggatgccag caaaagtaag gaatatcaag gcccagagac agacaagctt
    11941 ccagcaggtt gtccgctccg ttgacctcgt attctttgcc gtctacatga attgtagcca
    12001 ttagcatgct tccagttttc tcggtcaggg accgagcgtt aatcgaaatt cggttaccag
    12061 cgctctttca gcaggttcgg ctgaatccca ttaatcaaat gggtattgct gaacggctgt
    12121 ttgattcccg cctcaaattc ttcgcggaaa tatttgatgg cgctctgtaa cggctccact
    12181 gcaccaggtg cgtgggcaca gaaagtttta cccgggccta agaatcgaca cagttgctca
    12241 agtgtttcga tatcgcccgg ctggccttcg ccacgctcca gcgcacgcag aattttcacg
    12301 ctccacggca gaccgtcgcg gcacggcgta caccagccgc aggactcacg ggcgaaaaac
    12361 tcttccaggt tacgcaccag cgacaccatg ttgatctcat ggtcaaccgc catcgccagc
    12421 gccgtaccca gacggctgcc cgctttaccg atactttcga attccatcgg cagatcaagg
    12481 tgcgcttcgg tcaggaagtc agtccccgcg ccgcctggct gccaggcttt aaatttcaga
    12541 ccatcacgca taccaccggc gtaatcttcg aggatctcgc gtgcggtggt gccgaacggc
    12601 agttcccaca gtcccggatt tttcacccga ccggagaagc ccatcagctt ggtgccagca
    12661 tctttacttt tcgagatgtt ctgataccac tccacgccgt tagcgaggat cgccggaacg
    12721 ttacacaggg tttcgacgtt gttgacacag gtcggtttgc cccatacgcc ggaggttgcc
    12781 gggaaggggg gtttcgagcg tgggttagca cgacgccctt ccagggagtt gattaacgct
    12841 gtttcttccc cgcagatgta gcgccctgcc ccggtatgga cgaacagttc gaagtcgaaa
    12901 cctgttccca taatgttttt gccaagcaag cccgcttcag tggcttcggc aatggcgcgg
    12961 cgcagattta ctgccgcttc gatatattcg ccacgcagga agatgtagcc acggtaagct
    13021 ttcagcgcaa acgcggagat gagcatacct tccaccagca ggtgcggcag ttgctccatc
    13081 aacaggcggt ctttataggt gcccggctcc atttcatcgg cgttacacag caggtaacgg
    13141 atgttcatgg attcgtcttt cggcatcagg ctccacttca ggccagtcga aaagcccgcg
    13201 ccgccgcgcc ctttcagacc agcgtctttt acctgattaa cgatttcgtc cggagacagc
    13261 ccggtcagcg ccttacgcgc cccttcgtaa ccgtttttgc tgcggtattc gtccagccac
    13321 actggctgtt tgtcatcgcg cagacgccag gtcagcggat gcgtttcggg agtacggata
    13381 atgtttttca tttataccgc tccagcagtt cagggatcgc ttccggggtc agatgcgcgt
    13441 gagtgtcctc atcgatcatc atgtttggcc ctttatcaca gttccccagg cagcaagttg
    13501 gcagcagcgt aaagcggcca tcaaatgtcg tttgccctgg tttgatgttc agctttttct
    13561 cgagcgccgc ctgaataccc tgatacccgt tgatatgaca gaccacgctg tcgcaataac
    13621 ggatcacatg gcgaccaacc ggctggcgga agatctgact gtagaacgtt gccacacctt
    13681 cgacgtcgct tgccggaata cccagcacat cggcgatcgc gtggatcgca ccatccggca
    13741 cccagccacg ctgcttctga acgattttca gcgcttcaat ggacgccgca cgcgggtctt
    13801 cgtagtggtg catctcgtgc tcaatcgctt cacgctctgc cgcactcagc tcaaaagcct
    13861 cggtttgtgg ttgttgattc tcgtgcataa ttagcggtcc acatctgaca taacaaaatc
    13921 gatactgccc agataaacaa tcaggtcaga caccaggctg ccgcggatcg ccgccggaat
    13981 ttgctgcaaa tgcgcataac tcggcgtgcg gatacgagtg cgataactca tggtgctacc
    14041 gtcgctggtc aggtagtaac tgttgatccc cttggtcgcc tcaatcatct ggaaagattc
    14101 attggcaggc atcaccggcc cccacgacac ttgcaggaag tgggtgatca gggtttcgat
    14161 atgttgcagc gtgcgctctt tcggcggcgg cgtggtcagc gggtgatccg ctttgaacgg
    14221 gccttccggc atgttgttga ggcactgctc aagaatgcgc agactctggc gcagctcttc
    14281 cactttcagc attacgcggg tgtagcagtc agaaacacca ccaccaaccg ggatttcaaa
    14341 gtcgaagttt tcatagccag aataaggacg cgccttacgc acgtcgaagt cgatcccggt
    14401 agcacgcagg cccgcgccag tggtgcccca ctccagcgcc tctttcgcgc cataggcggc
    14461 aacgccctgg gaacgacctt tcagaatggt attttgcagc gccgctttct cgtaagacgc
    14521 cagacgtttc ggcatccagt cgaggaactc acgcagcaga cgatcccagc cgcgcggcag
    14581 gtcgtgcgct acgccgccaa tacggaacca cgccgggtgc atacggaaac ccgtgatcgc
    14641 ttccaccaga tcgtaaattt tctgacgatc ggtaaaggcg aagaacacgg gcgtcattgc
    14701 gccgacgtcc tgaataaagg tcgagatgta cagcaggtgg ctgttgatac ggaacagttc
    14761 ggagagcata acgcgaatga cgttaacgcg atccggcacg gtgatcccgg ccagtttctc
    14821 taccgccagc acgtaaggca tttcgttaac gcagccgccg aggtattcaa tacggtcggt
    14881 atacggaatg tagctgtgcc aggactggcg ctcgcccatt ttctccgcac cacggtggtg
    14941 gtagccgatg tctggtacgc agtcgacaat ctcttcgccg tcgagttgta aaacgatacg
    15001 gaaagccccg tgcgccgacg ggtggttcgg accgaggttg aggaacatga agtcctcatt
    15061 ttcggtgccg cgtttcatcc cccactcttc cggtttgaag gtcagggctt ccatctccag
    15121 atcctgtttg gctttggtca gctcaaacgg cgagaattcg gtagcgcgtg ccgggtaatc
    15181 tttacgcagc gggtgacctt tccaggtttg cggcatcatg atgcggcgca ggttcgggtg
    15241 accgtcgaaa gtaatgccaa acagatccca ggtttcacgc tcataccagt tagcgttcgg
    15301 gaacagtttg gtgaaggtcg gtacgtgcag gtcgttttct gccagcgcca ccttcagcat
    15361 gatgtcgcgg ttacgatcga tagaaatcag atggtagaaa acggaaaaat ccgcggcagg
    15421 taacccttcg cggtgtgtgc gcagacgttc gtccatgccg tgtaagtcaa acagcatgac
    15481 gtaaggtttc ggcagtttct ttaagaaatc gccaacttcc agtaattgtt cacgcttgat
    15541 ccacacaacg ggaaccccgg tgcgagtcgc ctgaacagta aaggcatccg gcccaaaacg
    15601 gttgcgcagt tcgccaatta ccggatcatc aagatgatcg cgggtctgcc aggcgggttc
    15661 ttgcgcggtt aagtcggtca tattgttcac cattgcaaat ggtccgtggt gactgtcggc
    15721 gcagtaagct tcgcgaaata gatttaggaa taagcgaaga aaaatccctt tgccgacagg
    15781 cgcaaattaa atctcgtcag gtgtacgcag gttggttacg gcaatgcgtt caccgcgctt
    15841 gcgttcgcgc tctgattgca tattggcgcg ataaacgccc tgatcgccaa ccacccagga
    15901 gagcggacga cgttctttgc cgatagattc ctgcaacagc atcagtgcct gcatgtacgc
    15961 ttcaggacgc ggcgggcagc ccgggatata cacatcaacc gggatgaatt tatcgacgcc
    16021 ctgcacaacg gaataaatat cgtacatacc accagagttg gcacaggcac ccattgagat
    16081 aacccatttt ggttccagca tctggtcata cagacgctga ataaccggtg ccattttggt
    16141 aaagcaggtt cctgcaacca ccatcaggtc agcctgacgc ggcgaagcac gcaatacttc
    16201 tgcgccaaaa cgcgccacgt catgcaccgc ggtaaacgaa gtcaccatct caacgtaaca
    16261 gcaggaaaga ccgaagttat acggccaaat tgagttttta cgaccccagt taaccatgtc
    16321 attgagcttg cccataaaca cgtttttgtt aacttcttgc tccagagggt cggttacgat
    16381 ctcctgcttt tgcagggggt aacggtcgtt ctcaccgttg ggatctatgc gggtgagcgt
    16441 ataatccatc ttaatgcctc gcggttagcg ttgacgatta gcgatactgt tcgtttccgg
    16501 gttcatacgc tcgcggcgtg aacgcgcggg cgtccagtcc agcgcgccaa tacgcaccag
    16561 ataaaccaga cctgccagta acacaaaaat aaaaattgca gcttccacaa agcctaccca
    16621 gccgctttcg cggatagagg ttgaccatgc gaacagatac agcgcttcaa cgtcgaagat
    16681 aacgaagaac atggccacca gataaaactt ggcggacagg cgtaagcggg cggagccgac
    16741 cgagtcgata ccggattcaa acggcacgtt tttcgacctc gcgcgtgcgc gaccgcctaa
    16801 aaaccaaccg cctaccagca tcaggcaaca caggccaatg gcaacgataa gaaagatagc
    16861 gaatgcccag tgatgagcga tgacttcagt ggatgttgac atactcattg cttactcatc
    16921 aaaagtagcg ccagattcac tgctcttcac ggcagatgga cgccacatcg attcatgggg
    16981 aggaataaaa aaaaccttac aataactgta gaaattcttt tatacagcta attgatgtgg
    17041 tcttttactc ctttctataa ccttttgtca actttaacaa aagcttcttc acattagttt
    17101 acataatatc aacaccatta gcatttaatg ccctttcacc ccagatcctt gacgactcca
    17161 ggataattag atgttgttga atcgtgtccg ttgtgaagca atggaaaaaa tacgggtcta
    17221 ttttgacagg aatttgtgtc gattcctccc cccaaaagag agtattttct tgatctgtga
    17281 cacgcttttg ttattccata acaaaaacgc agcaacaaat ttacgtattt tttaacatca
    17341 ttgtagcagg tgattttttt caggcgatta tttgtgcatt cgggacgtga atctctggtg
    17401 attgaaaaat aaacagtttt gtacgttctg cactatgcga ggaaggattt ttactaaaaa
    17461 aaagccgctg gggtttaaaa cacccccagc ggctcgtttt ttacactatt gtctcaggaa
    17521 ttatctatcg tccgtcgatt actcgatatc cctttcaatc aacaatgaat catccccttc
    17581 cggagcagac attgggctgt attgccacgg attatggtag ctctccatcg cctgatagat
    17641 cacctgggcc agctcattat tactggacgg gtcatagcac agcaaatatt cggtatcagg
    17701 tagcggcggt aagccatcta ctccactcag cacacgcagg tccgggctca tcatctcaac
    17761 cggccttgcc gtcacgccaa gaccggcttt cactgccgca cgaacggccg gaagcgtcga
    17821 ggcgacataa gccagtcgcc atggaatatc tactttatta agcgtcgcca gcaccatatc
    17881 gcgaaacgga cttggatcat ccagcaatac aagagggatc ggctcgcctt tttgcaatac
    17941 gtattccgct gcgcagtacc agtgtgttgg cgaggtacgc aggttcaatg ccttaaacgc
    18001 tgaaggacga tgggtggtta ccatcaaatc cacttcctgg gattccagca tttctgccat
    18061 ataggcatta cgtttaacgc ggacatccag cgctaatttc ggataaaccg aactcacgcg
    18121 atttaacagg aaaggtaaga tcgtatcggc agattcatct gaagcaccga tagttaacac
    18181 gccctgaaga ttactgaaca ttaatgatga gcaggcctca tcattaaaac gcaggatttt
    18241 cctggcgtaa ccaagaagtt gaatgccatg ttcagttaac agtttgttgc gaccgtgacg
    18301 agcgaacagt tctttcccaa cgagttgttc cagacgctgc atttgctgac ttacggcgga
    18361 ctgagtacga cacacagcgg cagctgcggc agcaaaagtg ttcagatcgg caacagcaac
    18421 aaatgttctc agcagatcga ggtcgaggtt aattatcgga cgatttgcac ttatcatata
    18481 ttatcactta ctggcggctc atactgagct ggttaatgct gtgcacacac aaacaagcaa
    18541 ttccatttgt aatgtgcctc cctggcagtt tcatccggaa cccggacgaa agtaaaaatg
    18601 catatgagtt gcactaaaaa agcgactcac attgttccgt tataatgcct gaagtagatc
    18661 acagaatata tcttcaggga tcgcatatct attaagttac tcactctttt ctatttatga
    18721 catgcgcgtg tttgtataaa tgtaaatgtg agtccttgtt ccactctcgt gcagcatcgc
    18781 tggtcatacg cgaacacgta caaacagcaa tggtgaagcg gcatcaagag cagggatccg
    18841 tcatttatgc gagcatttta ccccaaaaac cttttattta taagggtcat tgcgaattat
    18901 ctgatgcaaa gttatgttat gttaggcaaa gtaatcttct attattaata agcacatcaa
    18961 aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt aattccgctt
    19021 aacaatagtt tcacacctta cccccattgc gacacctcgg aaatcatcac gctgttaaac
    19081 tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa atgaggcatt
    19141 ttcacacaat tatcttacag ataaaaaaac cagacttgca attaagaatt agaacaattc
    19201 actatataac attgcatgta aagcatataa gcctcattat ttgtcattat taagttatta
    19261 acagcacaat cgagccttcc cctctggcaa aatcttattc tgcagacctt caaaacaccg
    19321 tcctggggga gtacattgtt ctaagctgac ttccacggca gggagtggcg ataacagcaa
    19381 aaaaggtcaa gattcatgtc ccccattgaa aaatccagca aattagagaa tgtctgttat
    19441 gacatccgtg gtccggtgct gaaagaagca aaacgcctgg aagaagaagg taacaaggta
    19501 ctgaaactga acatcggcaa cccagcccca ttcggttttg acgcgccaga tgaaatcctc
    19561 gttgacgtga tacgcaacct gcctaccgct caagggtatt gcgattccaa agggctttac
    19621 tccgcgcgta aagccatcat gcagcactac caggctcgtg gcatgcgtga tgttaccgtg
    19681 gaagatattt acatcggcaa tggtgtatcg gagcttatcg ttcaggcaat gcaggcattg
    19741 ctgaacagcg gggacgaaat gttggttcct gcaccagatt acccactctg gaccgcggcg
    19801 gtttcgcttt ccagcggtaa agcggtgcat tatctttgcg atgaatcctc tgactggttc
    19861 ccggacctcg atgatattcg cgctaaaatt acgcctcgta cgcgtgggat tgttattatc
    19921 aacccaaata acccaaccgg cgcggtatat tccaaagagc ttttaatgga gattgtggag
    19981 attgcacgtc agcataatct cattatcttc gccgatgaaa tttatgacaa aattctctac
    20041 gacgatgctg agcatcactc aattgcgccg ctggcacctg acctgctgac cattaccttt
    20101 aacggactgt cgaaaacgta ccgcgttgca ggcttccgtc aggggtggat ggtgttgaac
    20161 gggccgaaaa aacacgccaa aggctacatc gaaggtctgg aaatgctggc ttcaatgcgc
    20221 ctgtgtgcta acgttcctgc gcaacacgcc attcagaccg cgctgggtgg ttatcagagc
    20281 atcagtgaat ttattacccc tggcggtcgt ctttatgagc agcgtaaccg cgcgtgggaa
    20341 ctgatcaacg atattccggg cgtttcctgc gtgaaacctc gtggtgcgct gtatatgttc
    20401 ccgaaaatcg acgccaaacg ctttaacatt catgacgatc agaaaatggt gctggatttc
    20461 ctgttgcagg aaaaagttct gttggtgcaa gggacggcat tcaactggcc gtggccggat
    20521 cacttccgca ttgtcacgct accgcgtgtc gatgatatcg agctgtcttt gagcaagttc
    20581 gcgcgtttcc tttctggtta tcatcagctg taatcttaat ttcactgccg gagattgcat
    20641 ccggcagtgt tatcccgcca caatgacctg atgatgtcat catacgtaag gtcactatga
    20701 aacagagcca tttttttgcc catctctccc gcctgaaact cattaaccgc tggccgctaa
    20761 tgcgcaacgt gcggacggaa aatgtgtccg aacacagttt gcaggtagcg atggtcgccc
    20821 atgcgctggc agctatcaaa aatcggaaat ttggcggtaa tgtcaacgcc gaacgtatcg
    20881 ctttactggc gatgtaccac gatgcctcag aagtgctcac cggcgatctc cctactccgg
    20941 tgaaatactt caatgcgcaa atcgctcagg agtacaaggc tattgaaaaa atcgctcagc
    21001 agaaactggt cgatatggtt ccggaagaac tgcgggatat ctttgcgccg ttaattgacg
    21061 agcatgcata tagcgatgaa gaaaaatcgc tggtgaaaca ggcagatgca ctgtgtgcat
    21121 atctgaaatg tctggaagaa ctcgcggccg gaaataatga attcttgctg gcaaaaacgc
    21181 gactggaagc gacgcttgaa gcgcgtcgct tccaggagat ggactacttc atggaagtat
    21241 ttgttcccag cttccatctt tcgctcgatg agattagcca ggattcaccg ctgtaagcag
    21301 ccggagtctg cgtcgcatca gtcaataagc gccggatgcg acatcaggct cttgtcaaaa
    21361 cggaaacagc atcgggatca tcaccacaca aaccgccatc acgataatgg tgaacggtac
    21421 ccccaacttc acaaagtcac tgaagctgta atttcccgga cctaaaacca gggtgttaac
    21481 aggtgaagac accggtgtca taaaggcggc ggatgctgcc atcgccacga ccatcgcaaa
    21541 tggataaggc gacactccca tcgttttggc agcagccagc gcaatcggag ccatcaacac
    21601 cgccgtcgcg gtattagaga taaacagccc aataaccgcc gacaagacaa acaaacaccc
    21661 cagcatcata tgtggcccat aaccgccgcc aatatccatc agccctttca ccgccagcgc
    21721 gacacctccc gttttctgta atgccacagc aaacggcatc atcccaacga tcaaaataat
    21781 gctcggccag tgaatggatt tataggcgct ttcagcatct atacagcgga atttccccat
    21841 cagcaggcag gcgatgatag cggcaacagg attaggaatt tcatttgtca gcattaacgc
    21901 caccattagc accagacaga aaatggcatg gggtgcctgg ctgtgcgcgg gcgatgcttc
    21961 actcacctct tccggtaagt tcagcgctac gaagtcgcgg ccctgtttgg ccagcatacc
    22021 gatcagtttc cagttaccca caaccaggat gatatcgccc agcagcagag gctcatccgc
    22081 cagcgaacct tccagcgcca cgccattgcg cttgagcccc accacattca gtccgtagcg
    22141 ggtacgaaaa ccaatttcgc gcaccgattt accaatcagt tctgactcag gaattaatga
    22201 aatctctgcc atgcccacat caagggcctg gtcagaaaaa tactcgccgc gcagtaccat
    22261 cggctccagc aattgctcac tacaaaattg ccggagatcg acatcagccg cagacatatc
    22321 aataagcaaa acgtcacgcg cgcgaaattc agaaacccca ttaacgttca cgataacgcg
    22381 acgaaaacgc cgccagcgtt caacaccgat gacgttagcg ccataacgct cacgtaattt
    22441 gagatcatcc agccgttgac ctatcattgg cgatccgggg cgaatagcca gacgtcgcgc
    22501 acgcccggtc agtcgatatt cacggataag atcgcgaaag gttcgacgcg tccagccttc
    22561 gcgctgcggg gtctgggtat cccctttcag catgaaacgc atcactaaca tatacaaaat
    22621 acccagcacc agcacgacca ggccaatagg tgttacgcta aagaaactga agccgtgata
    22681 gccttcacgc agcaattcac tgttgactac caggttcggc ggcgtcgcca ccagcgtcat
    22741 catgccgcta atcagcccgg caaaacttaa cggcatcatc agacgtgacg gcgaagtttg
    22801 catacgcatg gcaacgctta acaccacggg aataaagata gcgacaacgc tggttgaact
    22861 cataaacgcg ccaagccccg cgacggtcag catcaacaaa accaacattt tgatttcact
    22921 gttgcccgcc actttgacca gccatgttcc cattacggtg gcaacaccgg tacggaccaa
    22981 accatcgcca ataataaaca aggcggcaat caggacaacg ttaggatcag aaaagccgga
    23041 aaatacttcg gggaccgtca gcgttccgct taatgcaaac gcgacaataa caaacaaagc
    23101 gaccgcatcc atacgcactc tgcccgtcgc aaacaagacg atggcaacgg ccagtaatga
    23161 aagaacccaa atcaattcac cgttcacaac ttatccttgt taattgaggg ggatgacttg
    23221 attctgccat aaaaaagccc cgacgagacg gggctaaatc atgatcaggt gtttcactga
    23281 ataataacat cgccatttgg ctgtttggtc acagtaattt gctccagact gtggaggacc
    23341 aaatcgacct cattcaggcg cggggtatct gccggagcgt taaccgcaat gacatggcaa
    23401 cccgccgcca ggccagaaag cacgccagcg ggagcatctt ccaccaccac acactcctgc
    23461 ggcgcaagcc ccagcagctg cgcacctaac agatacgcat caggttctgg ttttccgcgc
    23521 ttcactcgct cagcggttac aaacacctct ggtgcgggaa gccccgctat tttatggcgc
    23581 gctcgcgcta ccggcatgga gccagaagtc acaatggccc acggaatacc tgctttattc
    23641 aaatgattga gtaaggcgat tgccccagga agcgcggtaa taccttcggt ttccgtggcc
    23701 tcgatttgct ccagacgcgt aaactcggcg gcaatatcag cctcggattt gcccgccata
    23761 aaatggcgca aagaggtgat cgcctgttta ccgtgaatga aagccagcac ctcttccggc
    23821 gctaacccat gacgtctggc ccagttgctc cacgcccgtt ctaccgcagg cagggaatcc
    23881 accagcgttc catcaagatc aaacagaaaa cctttgcacc gcacgcgggc ctcctcaggc
    23941 gttgataatt tgattaattt cgttggcgct caaatggtac tgacgcgggc aggcatgcca
    24001 cacattaagc atgcgctgat atttttccca cattggcgtc tgggcgttaa aaccatgagt
    24061 cccagcgtca aaatgggtat agcgcccttc cacattaacc ataaagcgga cataaccgag
    24121 gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg acacgacgtt catcgatgga
    24181 ttgctggtcc tgcaaattag accaagaaac atacaacgca tgatacatat ccataatgtc
    24241 gatgatagtg cggcaggttt cttctttcag ctcgccaaac tcgcgatcca attcacgcat
    24301 ctgtaatccg taaccacgct caataattgt ttgcaggcga cggtaacgtt cagcatttgc
    24361 cggatcgagc atagtcatca tcttgtactg attagacaaa ataagacgtt gcgcgttggt
    24421 catttccatt gttgactcct gtatcactct actacggtga aaaaaaagaa ggctgagtat
    24481 gccttctttt atatgcgtaa tcaggggtca attacaaatc atcaaggaaa gttttatcca
    24541 gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg gtaatcaggc ttgccttcaa
    24601 cgggtgccaa cacctgtcca gactcctgca atttaccgcg aacttcataa aaccagttaa
    24661 ggattgcagg gggtaatggc gttacagaac gcttgcccag ccaccacaat ccctgcatgg
    24721 gtaaacttaa ggcgaacagt gcagtggcaa ctgccggccc aagcagaccg cccagggcaa
    24781 tctgccagca gagagtaaat acggcgatcg gcggcataaa acggatcgca taacgcgtca
    24841 tcttgataac gcgattttcg acaaagaccg gagcaaggcg tttttccagt ggccacgtct
    24901 ttgagtaatg ctgtccgcgg cgaaacaagc taaaaaaatt aacagaacga ttatccggcg
    24961 ttgacatgct tcacctcaac ttcacatata aagattcaaa aatttgtgca aattcacaac
    25021 tcagcgggac aacgttcaaa acattttgtc ttccataccc actatcaggt atcctttagc
    25081 agcctgaagg cctaagtagt acatattcat tgagtcgtca aattcatata cattatgcca
    25141 ttggctgaaa attacgcaaa atggcataga ctcaagatat ttcttccatc atgcaaaaaa
    25201 aatttgcagt gcatgatgtt aatcataaat gtcggtgtca tcatgcgcta cgctctatgg
    25261 ctccctgacg tttttttagc cacgtatcaa ttataggtac ttccatgtcg agtaagttag
    25321 tactggttct gaactgcggt agttcttcac tgaaatttgc catcatcgat gcagtaaatg
    25381 gtgaagagta cctttctggt ttagccgaat gtttccacct gcccgaagca cgtatcaaat
    25441 ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc aggcgccgct cacagcgaag
    25501 cgctcaactt tatcgttaat actattctgg cacaaaaacc agaactgtct gcgcagctga
    25561 ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta taccagctcc gtagtgatcg
    25621 atgagtctgt tattcagggt atcaaagatg cagcttcttt tgcaccgctg cacaacccgg
    25681 ctcacctgat cggtatcgaa gaagctctga aatctttccc acagctgaaa gacaaaaacg
    25741 ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga agagtcttac ctctacgccc
    25801 tgccgtacaa cctgtacaaa gagcacggca tccgtcgtta cggcgcgcac ggcaccagcc
    25861 acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa accggtagaa gaactgaaca
    25921 tcatcacctg ccacctgggc aacggtggtt ccgtttctgc tatccgcaac ggtaaatgcg
    25981 ttgacacctc tatgggtctg accccgctgg aaggtctggt catgggtacc cgttctggtg
    26041 atatcgatcc ggcgatcatc ttccacctgc acgacaccct gggcatgagc gttgacgcaa
    26101 tcaacaaact gctgaccaaa gagtctggcc tgctgggtct gaccgaagtg accagcgact
    26161 gccgctatgt tgaagacaac tacgcgacga aagaagacgc gaagcgcgca atggacgttt
    26221 actgccaccg cctggcgaaa tacatcggtg cctacactgc gctgatggat ggtcgtctgg
    26281 acgctgttgc attcactggt ggtatcggtg aaaatgccgc gatggttcgt gaactgtctc
    26341 tgggcaaact gggcgtgctg ggctttgaag ttgatcatga acgcaacctg gctgcacgtt
    26401 tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc tgcggtggtt atcccaacca
    26461 acgaagaact ggttatcgcg caagacgcga gccgcctgac tgcctgattt cacaccgcca
    26521 gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc ggtaacgaaa gaggataaac
    26581 cgtgtcccgt attattatgc tgatccctac cggaaccagc gtcggtctga ccagcgtcag
    26641 ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt ctgagcgttt tcaaacctat
    26701 cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact acgactatcg tgcgtgcgaa
    26761 ctcttccacc acgacggccg ctgaaccgct gaaaatgagc tacgttgaag gtctgctttc
    26821 cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca aactaccacg ctaacaccaa
    26881 agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca cgtaagcacc agtttgccca
    26941 gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa atcgtcttcg ttatgtctca
    27001 gggcactgac accccggaac agctgaaaga gcgtatcgaa ctgacccgca acagcttcgg
    27061 tggtgccaaa aacaccaaca tcaccggcgt tatcgttaac aaactgaacg caccggttga
    27121 tgaacagggt cgtactcgcc cggatctgtc cgagattttc gacgactctt ccaaagctaa
    27181 agtaaacaat gttgatccgg cgaagctgca agaatctagc ccgctgccgg ttctcggcgc
    27241 tgtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc gatatggctc gccacctgaa
    27301 tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc gttaaatccg tcactttctg
    27361 cgcacgcagc attccgcaca tgctggagca cttccgtgcc ggttctctgc tggtgacttc
    27421 cgcagaccgt cctgacgtgc tggtggccgc ttgcctggca gccatgaacg gcgtagaaat
    27481 cggtgccctg ctgctgactg gcggctacga aatggacgcg cgcatttcta aactgtgcga
    27541 acgtgctttc gctaccggcc tgccggtatt tatggtgaac accaacacct ggcagacctc
    27601 tctgagcctg cagagcttca acctggaagt tccggttgac gatcacgaac gtatcgagaa
    27661 agttcaggaa tacgttgcta actacatcaa cgctgactgg atcgaatctc tgactgccac
    27721 ttctgagcgc agccgtcgtc tgtctccgcc agcgttccgt tatcagctga ctgaactggc
    27781 gcgcaaagcg ggcaaacgta tcgttctgcc ggaaggtgac gaaccgcgta ccgttaaagc
    27841 agccgctatc tgtgctgaac gtggtatcgc aacttgcgta ctgctgggta atccggcaga
    27901 gatcaaccgt gttgcagcgt ctcagggtgt agaactaggt gcaggcattg aaatcgttga
    27961 tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc gagctgcgta agaacaaagg
    28021 catgaccgaa accgttgccc gcgaacagct ggaagacaac gtggtgctcg gtacgctgat
    28081 gctggaacag gatgaagttg atggtctggt ttccggtgct gttcacacta ccgcaaacac
    28141 catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc agctccctgg tatcttccgt
    28201 gttcttcatg ctgctgccgg aacaggttta cgtttacggt gactgtgcga tcaacccgga
    28261 tccgaccgct gaacagctgg cagaaatcgc gattcagtcc gctgattccg ctgcggcctt
    28321 cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc ggtacttctg gtgcaggtag
    28381 cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag gaaaaacgtc ctgacctgat
    28441 gatcgacggt ccgctgcagt acgacgctgc ggtaatggct gacgttgcga aatccaaagc
    28501 gccgaactct ccggttgcag gtcgcgctac cgtgttcatc ttcccggatc tgaacaccgg
    28561 taacaccacc tacaaagcgg tacagcgttc tgccgacctg atctccatcg ggccgatgct
    28621 gcagggtatg cgcaaaccgg ttaacgacct gtcccgtggc gcactggttg acgatatcgt
    28681 ctacaccatc gcgctgactg cgattcagtc tgcacagcag cagtaatctc gtcatcatcc
    28741 acagctttgc gctgcggata tctgaaccgg aaataatcac tatttccggt tttttattct
    28801 cttaatttgc attaatcctt tctgattatc ttgcttaact gcgctgcatc aatgaattgc
    28861 gccatctcac tttgcatact taccactttg ttttgtgcaa gggaatattt gcgctatgtc
    28921 cgcaatcact gaatccaaac caacaagaag atgggcaatg cccgatacgt tggtgattat
    28981 cttttttgtt gctattttaa ccagtcttgc cacctgggta gttccggtgg ggatgtttga
    29041 cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa acacgcaaag tcgtagatcc
    29101 acactcattt cgcattctga ctaacgaagc aggcgaacct gagtatcacc gcgtacagct
    29161 gttcacgacg ggcgatgaac gcccgggcct gatgaacttc ccgtttgaag ggttaacctc
    29221 aggatcgaaa tacgggacag ccgttggcat catcatgttt atgctggtga ttggcggcgc
    29281 gtttggcatt gtgatgcgta caggaaccat tgataacggt atcctggcgc ttattcgcca
    29341 tacccgcggg aatgaaattc tctttattcc tgcgctgttt attctgtttt cacttggcgg
    29401 cgcggtattt ggtatgggag aagaggccgt cgcctttgcc attatcatcg caccgctaat
    29461 ggtccggctg ggctatgaca gtattaccac cgtcctggtg acttatattg ccacgcaaat
    29521 cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtt gttgctcagg ggattgccgg
    29581 cgttccggtg ctttctggct ccgggttgcg catcgtggtg tgggttatcg ccactctgat
    29641 tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa aagaatcctc ttctgtcacg
    29701 cgtgcatgag tccgaccgct tctttcgtga aaagcaggcg gatgttgaac aacgtccgtt
    29761 tacctttggt gactggctgg tattgattgt cctgacagcc gtaatggtct gggtgatttg
    29821 gggcgtgatc gttaatgcct ggtttattcc agaaattgcc agccagttct tcaccatggg
    29881 tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac ggcatgacgg ttaataccat
    29941 ggcttcatcc tttaccgaag gggcgcgaat gatgatcgcc cctgccctgc tggtgggttt
    30001 cgccaaaggg attttgctgc tggttggtaa tggtgaagca ggtaatgcca gcgtgttaaa
    30061 tactatcctc aacagcattg ccaatgccat tagcggtctg gacaacgcgg tcgcggcctg
    30121 gtttatgttg ctcttccagg ctgtatttaa tttcttcgtg acgtccggtt ctggtcaggc
    30181 ggcattaact atgccgttac tggcaccgct tggcgatctg gtcggtgtta accgtcaggt
    30241 taccgtgctg gcgttccagt ttggtgatgg cttcagccac atcatttacc caacctcagc
    30301 ttcgttaatg gcaacgctcg gtgtgtgccg ggtggacttc cgtaactggc tgaaggtggg
    30361 cgcgaccctg cttggactgc tgtttattat gtccagcgtc gtagtgatcg gcgctcagtt
    30421 gatgggctac cactaaaaat gttaagagcc gcaattgcgg ctcttttttc attctgccgt
    30481 ttcagtctct acggcttcat ttttggcatt gcgcttcatc cacaacgcca gcgctttcag
    30541 cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct tccggcgtca gccagcaaac
    30601 tccactgact tcatcttcct gtagggcgaa gggaccgtga gagacgcaac tgaacaacgc
    30661 gccccagaca cggcaatttt tatcttcgaa atagaactgc ccgtgctcgg caaaggggac
    30721 accggcaatg cccaactctt cttccgcttc gcgacgcgcg gattccagca gttgctcatc
    30781 ggcctgaact acaccgcctg cggtcgcatc taacatgccg ggtaaaaagt cttttgtctc
    30841 ggtacgacgc tggaccagaa ttttgcccat gccatcatgc acgacgatgt aagttgcacg
    30901 atgacgcaga cactgtgccc gcatttgttc ccggctggct tgtgcaatga cttcgttctc
    30961 ttcattgaca atatccaccc attcagtact tgccaaacga cgctgttcca ttatcaggaa
    31021 accttctttt tctggcgctc ttacggcgca ttttgagttg tgggtaaatt acggattaat
    31081 cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt acactaagta cgtcattatc
    31141 cagcatgcca taacccgccg gattaccgcc tttcggaata ctcaccgagc cggggttgaa
    31201 gtggaaaatc tccccccgct gttctgccac tggcagatgg gtatgaccgt acaccagcac
    31261 atcgttctgg tttaaagcag gtagattttc cgggccaaaa agatgaccat gcgtcaaaaa
    31321 cagacgttgt ttttccagta atacctgttg ccacggcgcg gttatcggga aatgcagcat
    31381 catttgatcc acttcgctgt cgcagttgcc acgcacagcg ataaccttat gtgccacttc
    31441 attaagccgt tcagcgactt tggctggcgc gtaaccctcc ggtaaagcat tacgcgggcc
    31501 gtgattcaac acgtcgccaa ggatcaccag ccactgggca ccgctttgga caaacaactc
    31561 cacaacacgt tccgtcgccg gtaacgaccc atgaatgtcc gatgcaaaca tcagtttcat
    31621 cactcactcc tcgtcgaaaa aacgtaagct atgatactgg attctgtcgt tgctatcagc
    31681 cagattgctt cgccgaaagt gcaataaaac gctggacaga cgctcgctgc cactggaatg
    31741 tcgcataatc caccaggcgt tctggcacct catcgccatg tagtaccagg cggttaatca
    31801 ttagcgccag atcagtatca gcaatgcacc attcaccaaa taaattcggc tgacccagtg
    31861 ctaacaaatg ttctgccatc gcgaacagtt tctctgcact ggcttttccc tcggccgtta
    31921 gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg acgctcttcg cggatgggca
    31981 tcagatcgct gcgcagccag gcctgaatct gtcgtgcacg cgcacgattt tctaaatcaa
    32041 gcggataaat acgttcccag gtcggtggcg caaatcgatc ttccagatac tccgcaatgg
    32101 cagaagattc actcaactca aaatcatcga tttgtaataa cggcacacgg cgtgtctgac
    32161 cgtaaccttg ccacgtcggc tgcaaatgtt caccgctgtc gaggtcgatg gtcttgatat
    32221 gaaacgacag gcctttttcc tgcaacgcca cccaggcgga taacacataa ggggagaaaa
    32281 agtgggcatc tgaccaaagc gtgatagcgg gtttactcat tatgtcctcg gcaataaagg
    32341 gggctggcta tcaaactata gagccttttt gtcactgtca cgtgacgaat actcacgtaa
    32401 gtcgccgcgc tttctatact tgtggtgttc agatgattaa acaaccggag ctgcaatgat
    32461 cgatctctat ttcgccccga cacccaatgg tcacaaaatt acgctgtttc tcgaagaagc
    32521 agagcttgat tatcgcttga ttaaggtaga cttggggaaa ggcggtcagt ttcgtccgga
    32581 atttttgcgc atttcgccca acaacaaaat tccggcaatt gttgatcatt ctcctgccga
    32641 tggcggcgaa ccgttaagcc tctttgaggc tgctgccatt ttgttgtatc tggctgagaa
    32701 aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc gccacattac agtggttatt
    32761 ctggcaggta ggcggactgg ggccgatgct tgggcaaaat catcatttta atcacgcagc
    32821 cccccaaacc attccttacg ctattgaacg ttatcaggtt gaaactcagc gtctttacca
    32881 tgtactaaac aagcggctgg aaaactcgcc ctggctggga ggcgagaact acagcattgc
    32941 ggatattgcc tgctggccgt gggttaatgc ctggactcgc ctgcgaattg acctcgcaat
    33001 gtatccggca gtcaagaact ggcatgagcg gatccgttcg cgccctgcca ccgggcaggc
    33061 actgctaaaa gcacaactcg gtgatgagcg ttcggatagt taacagaaac aggttctcgt
    33121 gtattatttc atcctaagta aaacaacgga gaacctgcaa tggcacaacc tgccgctatt
    33181 attcgtataa agaaccttcg tttgcgtacg tttatcggaa ttaaggaaga agaaattaac
    33241 aaccgtcagg atattgttat caatgtgacg atccactacc ccgccgataa agcgcgcact
    33301 agcgaagata tcaacgatgc gctgaattat cgcaccgtaa cgaaaaacat tattcagcat
    33361 gtagagaata accgtttctc tttgctggaa aaattaactc aggatgtgct cgatatcgca
    33421 cgtgaacatc actgggtgac gtatgctgaa gtggagatcg ataaactgca cgcgctgcgc
    33481 tacgccgatt cggtatccat gaccttaagc tggcagcgtt aatcgccata tcgggaggct
    33541 gcatgaacat agtgatcacc ggagggacgg gattaattgg tcgccatttg attccgcgtt
    33601 tgctggagct gggccatcaa attacggtag tgacgcgtaa cccgcagaaa gccagttccg
    33661 ttctcggccc tcgggtgaca ctatggcaag ggcttgccga tcaaagcaac ctcaacggcg
    33721 ttgatgcggt aatcaacctg gccggagaac cgattgctga taaacgctgg actcacaagc
    33781 aaaaagagcg tctctgccaa agccgctgga atatcacgca aaaactggtc gatttgatta
    33841 atgctagcga cacgccgccg tcggtactca tttccggctc ggcaacgggc tattatggcg
    33901 acttaggtga agtggtggtt accgaagagg aaccgccgca taacgaattt actcataaac
    33961 tctgcgcccg ctgggaagag attgcctgcc gggcgcaaag tgacaaaacg cgagtgtgcc
    34021 tgctgcgaac cggcgtggta ctggcaccgg acggcggtat tctcggtaaa atgctgccac
    34081 cgtttcgtct tggcctgggc gggccgattg gttccggtcg gcagtatctg gcctggattc
    34141 atattgatga tatggtcaac ggcattctct ggctgctgga taacgagctg cgcgggccat
    34201 ttaatatggt ttcgccctac ccggtacgca atgaacaatt tgcccatgcg ctcggtcatg
    34261 cgctgcatcg cccggctatt ttgcgcgtcc ccacaaccgc cattcggctg ttaatgggcg
    34321 aatcttcagt actggtatta ggtggacaac gcgcgctgcc taaacggctg gaagaagcgg
    34381 gttttgcgtt tcgctggtac gatttagaag aggcgctggc ggatgtcgtt cgctgatgtg
    34441 gtttacagca aacatccgcc agttaactcc ctgtgttaca ggattagtgg ctttgcgcga
    34501 taagatcgtc tggtgaaagt cgggtcacca tcataactaa ctctctgtct aaacctctat
    34561 ccagcatctc ctgagcaata cgcagggctt cttcgtgttt gccctgcatt gcgccttctt
    34621 cacgtaatct gtcagcaatg gtcatcagtt tctccttttc ttgtggtgcg cgttctgtta
    34681 tctcaccaat aaatgcacgg aaacgctggg catcccctgt ttgtaatacg taattaaaca
    34741 gggcttttag ctgtctgtca ttagtgttcc ctgtaactag cagcgaaaca atttggtcga
    34801 ctaatcccaa cagatcgcgc tgacgaatat gtttctgaat taactccaac agcgccattt
    34861 tgcggtgttg cataatctcg tcatccggca ccacggtaat atccaccaac ggaaacgctg
    34921 atgaatatat tttgcgggct atagcaggct cggcaaattc atcaagccag cagagtgaat
    34981 aaggataagg acttctgcaa ccatgataaa acagcatcgg gatcaccaat ggaagctctt
    35041 tatagcccgc atcaagatgg ttttgcattg ccgcaatgga ataacgcatc atgcgaaaag
    35101 ccattaattc ttccggctta ctttggtgct ctatcactac ataaatataa cccactccct
    35161 cctgcgtttt cacagaccac aagaggtcgg aataatattg ccgcaggtct tcatcaataa
    35221 aactgtttgg ttctagttta agcgtcgtta aatcacacag tttgcgcagc ggcgcgggaa
    35281 gatgaatatt aataaaattc cgcgcggtgt ctggatggcg taaaaaagat ttaaataccg
    35341 catcatgcgg cgtggaagtt gtcgatattg tcatggcgtt ccgtcacccc tcaaatttaa
    35401 gatgacgcga caataaccac gattaacacc caatgcagca ggcagttttt atctttggac
    35461 agcgaattca gaagtatttt tagcggactg caacttttac atttcagcgg aagctgactc
    35521 gcaaattccc cttgaaaata ctccggcagc gttagccacc ggagaggaaa ttatttcagc
    35581 gatcccttaa ggaactgttg cagacgaggg ctttgcgggt tgccaaataa ctgctccggc
    35641 gcgccctctt cttctatttt cccctgatgg aggaaaatga cgtgagtaga aacatggcga
    35701 gcaaagccca tttcgtgagt cactaccacc atggttttcc cctcttctgc cagttgctgc
    35761 ataatacgta acacttcgcc taccagttca ggatcgagcg ccgaggtagg ttcatcaaac
    35821 agtaaaactt ctggttccat cgccagcgcc cgcgcgatag aaacacgctg ttgctgaccg
    35881 ccggaaagat gcaccggata tttcccctgc gcacgttcgt ctatcccgac tttcgccaga
    35941 tacttcaccg cccgctcgcg cgcttcctgc ttgctcaggc ccaacacctg aatcggcgct
    36001 tccatgacgt tttccaacac cgtcatatgg ctccagagat tgaagtgctg gaataccatc
    36061 gtcaggcgtg tgcgcagtaa gcgcagttga tttttatcgg cgactttgag ttgaccgtct
    36121 ttgtcgcgca ccagattgat cgtctggcca ttgaccacga tcgatccttc actcggtttt
    36181 tcgaggaagt taatgcagcg cagaaaggta cttttccccg atcccgacga tccgatgatg
    36241 cttattacat ctccggcatt cgcttgcagt gagacccctt tcagcacttc gtgttcgcca
    36301 tagcgtttgt gcaaatcgat aacgtttaat ttattctcgg acatcgtatt ctcagtgcgt
    36361 tgaagaaggt ttcacatgct gcaaccagcg tttttccgct ctgcgaaaga ggctgatcag
    36421 gacataagag atgattaaat agagcaccgc ggcaatgccg aaggcggtaa aaggttgata
    36481 cgtggcggcg ttaatatcgc gggctatttt cagcagatcc ggcacagtgg ccgtaaacgc
    36541 cagcgcagta gagtgcagca tcaggatcac ttcgttgctg tatgccggta acgcaatacg
    36601 cagcgcagaa ggcaaaataa tgcagcgata cattttaaag gtcgagaagc cataggctct
    36661 ggcggcttca atttccccat gcggaaccga acggattgcc ccagcaaaaa tctcggtagt
    36721 gtaagcgcag gtgttaagcg tcagcgccag cacggtacag ttcaggccac tgcggaagaa
    36781 agcgttaagg aattcggttc ccttaacaat ctcaagcgtg tacatgccgg aatagaacac
    36841 cagcaactga acatacagcg gcgtaccgcg aaaaatatag gtaaataacc agattggaaa
    36901 ctggatgtat ttattactgg agacacgacc aatcgccaga aacagcgcca ggactccgcc
    36961 tatcactacc gacaaaataa gcagccacag agtgatcgcc acaccagtaa agcgataacc
    37021 gtcggtccac agcagcggtt tccagtattc atgtaagatt tcgatcacag gtcagccctc
    37081 ttcacaccca cggagtagcg gcgctcaagg aacagcagca caccattgga aacggtggtg
    37141 aaaaccaggt aaatcacgcc acagacgatg gcgaaataga acggttccca ggtacttttg
    37201 cctgccagct gcgtggcttt gaccacatct tccaggccga gtaacgaaac cagtgcggta
    37261 gatttgagga tcacctgcca gttgttgcca atgcctggca gggcgtaacg catcatcgcc
    37321 ggaaacatga tccgccgaaa cacttgcccg cgagtaaaac cgaacgccgt cgccgcctct
    37381 atatgccctt tcggcactgc cataaacgcg ccacggaacg tttcggtaaa ataagcaccg
    37441 tagataaaac cgagagtgat aataccagcg accatcggat cgatatcaat ctgcccgacg
    37501 cccatcgcct ccgtcaccat gtttagcgca atctgcaaac catagaaaat cagcagcatc
    37561 aacaccaagt ccggcacgcc tcgaatcagc gtggtgtacc cttcgaaaat cagccccgaa
    37621 agccgatttt gcgagagctt accgccagcg ccaattaaac cgatgattac agcgagcact
    37681 acagagctga tagccagctc cagcgtgacg agcgcaccct gtaaaataac acctgaaaac
    37741 ccatacaaca tgctgcctgt cctgtcgtgt gtggtgaatt accgcctctt gtctccctcc
    37801 ggttacccga agggagcggg ctttctttca ccgatggcga ttaaccacca taaacatcaa
    37861 aatcgaagta ctttttcgct aatttctcgt aagtaccgtc agcgcgcatt tcggcaaagg
    37921 ctttgttcag tgcctcgcgc agttcgttat cttctttacg caggcccatg ccggtcccta
    37981 cgccaaacag tttttcatct ttaacagacg gaccaccgaa tttgtaatct ttaccgacgg
    38041 gttgtttgag gaaaccttcg ctggcagcga cctcatcctg gaacgcggca tcaatacgtc
    38101 cggcagtcag gtcagaataa atgttgtcct gcccctgata cgagacgatt tcaatgcctt
    38161 ttggtgccca gtgttcatta ccgaacgtct cctgggtggt gccctgcaat acgcctaccc
    38221 gtttgccttt cagcgactcg actgtcggct gaatgtcaga atttttcgcc accaccagac
    38281 gagaatcggc agcgtacagt ttgtcggtga aggcaatttc ttgctgacgt ttttccgtaa
    38341 tggaaagcga tgacatgatg gcgtcaatct tcttcgcttt taaggacggg attaacgcat
    38401 ccagcgggtt ttcgacaaac gtacattgcg tattgatgcg cttgcacaac tccttcgcca
    38461 gctcgatatc gaaaccaacc agttcgcctt gtgaattttt tgattcaaat ggcgcatagg
    38521 tcgggtcggt accgatgcgg atgttttgcg gaattgcagc aagtgccgca gttgcgctgg
    38581 agaaggccag aaccagagag agcgatagca ccagtttttt cataatgtcc tcaactgaca
    38641 gtctttttat agggaatatt tacaggtttg ttacacttat cgtgccataa aattgaccat
    38701 caaggcaaaa tatcccgccc cgttagacgt attatttttc attaaaaatg cttaagtatg
    38761 catttcaatg caccacgata gtgcaacaaa cctggcgtgc accatgatga tcattccatc
    38821 aggtacagct tcccagcgac gtatcagtca ccgtagacat taaagtcgaa atactttttc
    38881 gccatcttgt cgtaggtgcc gtcctgacgc agctcgccaa gcgccttatt gaaggcagcc
    38941 gtcagttcag cataatcttt acgtagccct acaccggtgc catcaccgaa gtattttttg
    39001 tcttttactg atgagccagc aaaagcgaaa tctttaccag caggttgctt gaggaatcct
    39061 tcgctggcag caacttcatc ttgtaacgca gcatccagac gtcctgcagc cagatcggaa
    39121 tagaccaaat cctggttggc ataggccacc acatccacgc ctttactacg ccaggtctcg
    39181 ttagcgtaag cttcctgggt tgatccctgc agcacaccaa catgtttacc tttcagtgaa
    39241 tccagcgttg gctgaatcgg tgaacctttg gcagcaatca aacgagaatc tgcggcgtac
    39301 agcttgtcgg agaaggcaat ctcctgctga cgtttatcgg taatggaaag cgacgaaata
    39361 atagcgtcga tttttttcgc tttcagtgag gggatcagcg cgtcaaagtc actggcaacc
    39421 caggtacatt tcacctgcat ccgtttgcac atctcgttac cgagatcgat atcaaagcca
    39481 acaaaatcac ctttagcatc tttcgacgag aacagcgcat aggtggtatc ggttccgata
    39541 cgtaccgtct ccggtagcgc cgcatagctg gaagccgctg cggagagacc gactaataaa
    39601 gacagagcga gaatcgactt cttcatacat aaccctcaag tgaaaatggc ttttttatgt
    39661 tgtgtattgt gttgtgtgtt tgcaggctct ttcatgcagg tcttatgcca tcttgacgac
    39721 aacagtaaca ttcaacgtta aatatgttaa taagacattg cattattgtc ctgaagttga
    39781 agatagcagg tatagcggtt gaatcgcagc gtttcgattg aatggcagaa acaaaatgtc
    39841 gaggatttga tcgcggttgc aaaattgccc tgaaacaggg caacagcgga gttatgcgcc
    39901 ctgccagcgg gcaaagagat cttcaggaag ggttattgca aactggtcaa gaacacgatt
    39961 aaccgtctga tttatcacat catcaaggga ttgcgggcga tgataaaacg ccggaacggg
    40021 aggcataatc accgcaccga tttctgccgc ctgagtcatt aaacgcagat ggcctaagtg
    40081 caatggtgtt tcacgcacgc agagcaccaa cgggcgacgc tctttcagca ccacatctgc
    40141 cgcacgggtc agtaagccat cagtatagct atggacaatg ccggacaggg ttttgattga
    40201 acagggtaaa atcaccatcc ccagcgtctg gaaagaaccg gaagagatgc tggcggaaat
    40261 atcgcgcgca tcgagcgtga catcagctaa tgcctgcact tcgcgcaggg aaaaatccgt
    40321 ttcgagggat aaggtctggc gcgccgcctg gctcatcacc agatgcgttt cgatatctgt
    40381 gacatcgcgc agaacctgta ataagcgcac gccataaatc gcgccgctgg caccgctgat
    40441 gcctacaatg agtcgtttca taaatagttg ccctgtcaga cttgcaggca gactttgcag
    40501 tatttcggcg ggagttgcaa gtcagggtgc cagaccggca ccctcagcga aggcatcatc
    40561 cttcgttatg catttcgaga ttttccactt cgttctgacg ttgcactgct ttggcgtcat
    40621 cattacgtaa cgtatcgagg aaatcgaggt agccctgatc aacatctttg gtgacgtaga
    40681 cgccgttgaa caccgagcat tcaaactgct ggatatccgg attttcagcg cgaacggcgt
    40741 cgatcagatc gttcagatcc tggaaaatca acccgtcagc accgatgatc tggcgaattt
    40801 catcaacttc gcgaccgtga gcgatcagtt ccgtggcgct cggcatatca ataccataaa
    40861 cgttcgggaa gcgaatttcc ggtgccgcag aagcgaggta cactttcttc gctccggctt
    40921 cgcgtgccat ctcgataatc tgctcagaag tggtgccacg gacgatggag tcgtcgacca
    40981 gcaggacgtt tttatcgcgg aactcggcgc ggttggcatt cagtttacgg cgcacggact
    41041 tacgacgcag ctgctggccc ggcatgataa aggtgcggcc aacatagcgg tttttaacga
    41101 agccctggcg gtacggtttg cccagaatac gagcaatttc cagcgcgata tcacacgagg
    41161 tttctgggat cgggatcacc acgtcgatat ccagatcttc ccattcgcgg gcaattttct
    41221 cgcccagttt cgtgcccata ttcacacgcg cgctgtaaac ggaaattttg tcgataaacg
    41281 agtccgggcg ggcaaagtat acatactcaa acaggcacgg attgctgacc ggattgtcag
    41341 cacattgacg ggtaaacaac tgcccttctt cagtgatgta aatcgcttcg cccggcgcga
    41401 cgtcacgcag gaaatcaaag cccagcgtat cgagcgctac gctttcggaa gcgaccatat
    41461 attctgtacg gttctcgtca atatcacgtt ttcccagtac cagcggacga atcccgtttg
    41521 gatcgcggaa agcaaccata ccgtggccga taatcatcgc cacacaggca tacgcgccgc
    41581 ggattaagcg gtttgtggca gcaatggcag cgaaaatatt gtcggcttcc agcgggtagt
    41641 ggcggaagtt gtccagctcg ctggcgaaga tattaagcag aatttccgag tcggaagtgg
    41701 tgttgatgtg gcggcgtttt tcttcaaaca gttttttacg caactcgtga gcgttggtca
    41761 gattgccgtt gtgggcaagc gtaatgccat acggggagtt aacgtaaaac ggctgcgctt
    41821 cagaggcgct ggagctgcca gccgtagggt aacgcacatg accaatgccc atattgccct
    41881 gcaaacgctg catatggcga gcttcaaata catcgctcac cagcccgttc gctttacgca
    41941 aacggaagca gttattggca tctatggtga tgatgccggc ggcatcctga ccgcgatgct
    42001 gaagcaccgt taaggcatca taaatcgact ggttaaccgg cataacaccg gcgataccga
    42061 caataccgca catacgtctt tttcctcgtt aagccacatc tcagagcact tacgctctgg
    42121 gcaagaaact tgacgagctt tgcagataat caaaaaacca tctgatgata aaactgaatt
    42181 gcgggatcag ctgtgatttg ctccagtctt cacttttcga caccccggta aaggagtcga
    42241 gaaagaagag aatggcggca acaatcaaca caccgcgcaa cgcaccgaaa cagacgccca
    42301 gcacccgatc ggtgcctgac aaccccgttt tctccaccaa ctggcctatc acgaagttca
    42361 cgatagcacc aacgatcagg gtagcgataa acagtaccgc gatggcaatc ccatttcgaa
    42421 ccagttcgtc ttcaaagccc gtaaaccaga ctgacaggta agtgtagtaa tgactggcaa
    42481 caaagaaagc acaaccccat gtcaccagcg ataacgcttc acgaacaaag ccgtggatca
    42541 ggctaaccag agaggaaaaa gcaatcaccg cgattatggc gtaatcaatc cagaccatat
    42601 gtgtcccacg attttacgcc ctgtcatcct gttcggggcg cattctaaca gaaaaagaaa
    42661 acgtttgcgt agggatttcc ttcccgcgca tcaataaaaa tggcgctgaa aaaatattca
    42721 acgccatcga ctttttatgc ctttgcggca tcgtgcaatg cgtgtcggat gcggcgtaaa
    42781 cgccttatcc gacctacggt tctaacactg cccaagcctg ataagacgcg ccagcgtcgc
    42841 atcaggcaag actgtattaa ttcggcgtat agcccattac cacgccactt aagccagaaa
    42901 gttgcttcaa ctcacccagc gaacctttca gcttatcttt cgaggcatcc ggcccaacca
    42961 gaatacgggt aattttaccc tgcactggcg tggatggcga cgtataaacc cgataaccgg
    43021 caccgcgcag cttaccgaca atctcattca ctttatcggc atttttcagc gcacccagtt
    43081 gcacaacata ggctttaccc gttggcgctg ctttttcttc cacgaccggc ttcggttctg
    43141 gcttcggcgc aggtggtact tcaacctttg gtttaggcgg ctccaccggt ttcggcttcg
    43201 gtggggcgac cggtgcaggt tccggttcaa actcggtgtt attggctgca atagtggccg
    43261 gatcgagcga cggtgctgcg gcatcacctg cccgcacctc ttccgctgcg ccttccggcg
    43321 gctgcgtcgg caacgcctgg gtggcggctg gcatcatatc aggctcatca cgatcgcccg
    43381 ctttcggcac cagcgggata gccgcgaact catcctgata atgttttttc tgcccgtcca
    43441 gcagccctgg aagtacaatc acccccagcg ccaccagcac gatcgtgccc actaaccgat
    43501 tctgaaactt acttgccacc gcttctcctc gcgtcaatca cttccatgac atgtgcgacc
    43561 gtgtggaaag aaccacacac cagcacggtg tcttccgctt tagcgtccgc cattgcggca
    43621 tcccatgcct gcgcaacgct atcaaatgat ttgccgttac ccaaatgctc aagcagttgt
    43681 tctgccgtgg caccgcgcgg cccttccagt ggcgcacaat accagtcatc aaccacgctt
    43741 ttcaaccagg ccagagttcc ggcaatatct ttatcatgta gcataccgat aaccgccagc
    43801 acgcgcccgt ttttcggtag cgctttcata cgcccggtga gatattccgc cgcatgtgga
    43861 ttatgcgcga catcaaaaat aacgcgtggc gactcgctca caatctggaa acgtcccggc
    43921 aaaattgcgc tggcaatccc gtcgcgaatg gcattttcac tgacttccag cccgctggca
    43981 cgcagtgccg ccagcgccgt tgcggcattc ggttgcggga caagcggcaa cggcagattt
    44041 tccagcgtgc cgtgagcatc gctaaacgcc cagtcatgat cggtgacgga atagttccac
    44101 tcaacgcccc gacgttgtaa cagtgcacct ttttcctgcg ccacatcagc aatggtagaa
    44161 ggcatttccg gctcaccgac aattgccggt ttttcgctgc ggaagatgcc tgctttctcg
    44221 agaccaatac tttcgcgatc tggacccagc cagtcggtat gatccagcgc aatactggtt
    44281 actaccgcga catcggcatc gacaatattg gttgcgtcca gacgaccgcc cagccctact
    44341 tccagaatca ccacgtcaag ttgtgcctgc ttgaacagcc acaacgccga cagcgtaccg
    44401 tactcgaaat aggtcaggga aatatcaccg cgtgccgatt caatctccgc aaaagaggcg
    44461 gtgtgggccg attccggcaa ttcctggccc tgcacacgta cgcgctcggt ataacgcacc
    44521 agatgaggcg aactgtagac gcccactttg taccctgccg ccatcagaat cgactccagc
    44581 gtacggcagg tggtgccttt gccattcgta cccgcaacgg taaacacaaa tggcgctggt
    44641 ttcaggacgc caagacgcgc cgcgacctgg ctcacgcgct caaggccgag atcgatagtt
    44701 ttactgtgca ggttttccag ataagaaagc cacgaagcca gaggcgacgc ggcttgagga
    44761 gtgcgtttga taatcatggt atccgctgat tcgttacggt gagaatagca aaagggcaga
    44821 gccagtggcc ctgcccttat cagttatcag gcctcaggtt cctgatccgg taccgggggt
    44881 accactacgc cttcacgcgg cgcttcagga ttcggcgctg gcagattcat caacttcgcc
    44941 agaatgctcg ccagtttcag gcgcatttcc ggacgacgga cgatcatgtc gatcgcgcct
    45001 ttctcgatca ggaattcact gcgctggaat ctaggcggca gtttttcgcg aacggtctgt
    45061 tcgataacac gcggaccggc aaagccgatt aacgctttag gttcagcgat gttgagatcg
    45121 cccagcatgg cgaaacttgc agaaacaccg cccatcgtcg ggtcggtcag cacggagatg
    45181 tacggcaagc cgcgctcctg catttttgcc agtgccgcag aggttttcgc catctgcatc
    45241 agcgacatca gtgcttcctg catacgtgcg ccaccagagg cggagaagca gatcagcggg
    45301 cagttatctt cctgcgcctg ctcaacggca cgcacgaaac gtgcacccac aacagacccc
    45361 attgaaccgc ccataaaggc gaactcgaat gccgcagcga caaccggcat tccatacaga
    45421 gtgcctttca tcaccaccag cgcatctttt tcgccggttt ctttctgcgc agatgccaga
    45481 cggtctttat acttcttgga gtcacgaaac ttcagctcat ctttcggctc aagctcgcta
    45541 cccagctcca caaggcttcc ttcatctaac aggctatgca ggcgattacg cgctgtcata
    45601 cgcatgtgat ggtcacactt cggacagacc tcaagattac gttccagctc agcgcggtat
    45661 aaaacctgac cgcagctatc acacttagtc cacacccctt caggaatgct cgccttgcgg
    45721 gtgggagtaa tgttgctttt aattcgttca atccagctca ttagggacct ttctgtctga
    45781 acctggttcg atgccagttt tatctttggg gacgcataat gccatttttg cccccaacag
    45841 accatgaatg ttgcacatta aaacataaca gcccgaaact ttggataaaa aagtggtcga
    45901 accgcggagt tactttttat tttgcggcgc gtgccgcagc gcgtttgtga cggattattt
    45961 cgatgacgcc cggcaaaata gaaaccacaa taatcccgac gatcagcagc ttaaggttat
    46021 cctgaaccat cgggattgta ccgaagaaat agcctgcgta ggtaaaaagc agtacccaca
    46081 acagtgcgcc gatcacgtta taagcagcga aatgacggta cgacatgtgg cccattcccg
    46141 caacaaacgg ggcgaacgtt ctgacgatcg gcacaaaacg ggcgagaata atcgttttgc
    46201 cgccatgttt ctcataaaac tgatgggttt tgtcgagata actgcgacgg aaaattttcg
    46261 aattgggatt actgaataac ttttcaccga acaaccgccc gatagtgtaa ttgaccgcgt
    46321 cacccacaat cgcggcgatc agcatcagca ctaccatcat atggacgttg agatcgttgg
    46381 tttccagtga tgccaacgct ccggcaacaa acagcaatga atcacccggt aaaaacggca
    46441 ttaccaccag accggtttca cagaacaaaa ttaagaacaa aatggcataa acccagacgc
    46501 cgtactccgc gactagctct gccagatgca cgtcaatgtg caggataaaa tcgatgagga
    46561 aataaatcag gtccatattt gcctatgcct tgtactcgtc atctttcagg ctgtaactac
    46621 gttggctaca gcctgaaatg ttccgagcat tattctcgtt agtccgccag aaatagcggg
    46681 cccattggcg gttttggaag atcataccgg tcagggtaat ccaccgcgac cagatacagc
    46741 ccttccgctt ttgccgttgc tgccgccagc gttctgtcct ttgccgccag caactctgct
    46801 atccagctct ccggctggtt gtgggcaccg acttccatca ggctgccgac aatattcctg
    46861 accatatgat gtacaaaggc attcgctttg atatcaacca ccacataagg accgtgacgc
    46921 gtgacattaa tatgcataac gttgcgccac ggggttcggg actggcactg caccgcacgg
    46981 aacgaggtga aatcattctc gcccagcaag cattgcgcag cccgatgcat ccgttcagcg
    47041 tccagcggtt cgtaaaaatg ggttacccct ttactcagta ccgccgggcg cagccgatga
    47101 ttgtagatga tgtagcgata acggcgagcc gtggcgctaa atcgggcatg aaaatcatca
    47161 ggtacagctt taacccaacg cacggcgatg tcaccaggta aattcgcatt tacgcccaag
    47221 gtccacgccg cgtctttgcg ctgcgcggtt gtttcgaaat gcaccacttg cccggtaccg
    47281 tgtacccctg cgtcagtacg cccggcgcag aagacggtga tgggttcgtt cgccacctgg
    47341 gagagcgcct tttccagctt ctcctgcaca ctgcggactt cattctgccg ttgccagccg
    47401 taatacttac tgccgtcgta ctcaatgccc agcgcaattt tataaactgg cagttgttgc
    47461 tggtcggaca ttagtacaga tactcctgca ccagtttctc ggcgattttt actgccatca
    47521 gcgcgccgcc aaagcgaacg ttatcggcca ccgaccagaa ctggacttgc tccggcatac
    47581 cgtagtcatt acgcacgcag ccaacagaaa gatgcggcgt acccgaagca tcccctacct
    47641 gagtcgggaa ttcgttctct tcagagagca caatatcttc gccctgagca aacgcatcac
    47701 gcgcttcttc tgccgccagc ggacgcagtg cttcaaagtt gaccatctgg gcatgaccgt
    47761 agaataccgg tgcctggacg acgcttgccg aaatcatcag cccttcgtcc tgcaggattt
    47821 tgcgtacttc gtcaacgata cgacgttctt cacgcacgct accttcgcta tccggcaaca
    47881 acggcagcat attgaacgcc agttgacgac cgaagaaatc ttcttcgtca atcggaatgc
    47941 cgttgagcaa tttcgcactc tgccccgcta acgcatcgac cgcttttttg ccctgggcgg
    48001 aggctgaaat caggctggta acactgatac gcgacaaacc gccctgatcg attaacggtt
    48061 tcagtgccgc cagcagctgg ctggtcagac tgtctggtac ggcgatgaca ttccggttgc
    48121 ggtaatcggt cagtacaaac gggtttactt ccggcaccac cagcggtacg tcgggttcga
    48181 gagcaaacag tccgctgctg tcgatcacca ggcaacctga gttggtcgct tcttcaaccc
    48241 aggcagcggt agcttctttg cctgcgacaa aaaatgccag ctgcgcctgc gtccagtcga
    48301 attcagcggc atcctgcacg gtgattgttt taccaccaaa gcgcagttgt tcgcctgcgc
    48361 tttcgttacg tgccagtgca taaatttccc caaccgggaa ctgacgttca gccagcgttt
    48421 caagcagggc ttcgcccata gcgccagttg cgcccaggac ggcaatgttc cagccttcag
    48481 acatggtggt ttactccaga aatagcaaag ctccctgcca atgttacagc agagagcatg
    48541 aagaagagat taacgtgccg gatgatgaac ggcgttaaaa cccagtttac acagcaatga
    48601 tgccgcactg gcgtcatcac aaattacata cagagatgac cattcacggc gctcaagata
    48661 gtttttgcgc agtttatcga actcacccgg tatcccggcg actttacgca gcggtgcgtc
    48721 atcgcggcgc acatcataca ccaaatgcac cagccttttc agcgtcggtt gatcgagcgg
    48781 accatgcagc gtaatgcggc caaactcagg cgcgggcagc aacgtatcca gcgcaacgtg
    48841 ttgttcatgc ccaataaact tgctataggc ttcaaacact tgcgtcgtgc cacgcgcttt
    48901 accctccagg gtatagcctg cgatatgcgg cgtgccgata tccacttttg tcagcaattc
    48961 cacattaagt tccggctcgc cttcccagac atccagcacc acgcttaact tctggccttc
    49021 attcaggcag gtcagcagcg cggtattatc gacgactgcg ccacggcagg cgttaatcag
    49081 aatcgctccg ggcttcaggc tacggatcag tttttcatcc gccagatgta gcgttttgta
    49141 cggaccatct ttaaagagtg gcgtatggaa agtcagaata tccgcgcgct ggactaactc
    49201 atccagcgag cggaaatcac cctcatcccc acggtcggcg cgaggcggat cgcaaagtaa
    49261 ggttttaatc cctaacgctt ccagtcgcgc ctgcaatcga cgtccaacgt taccaacgcc
    49321 cacgatcccc accgtacggt cgtgcagtga aaatccatcg cgttcggcaa gcatcagcag
    49381 ggaggagaaa acatattcca ccaccgcaat cgcattacag ccaggtgcag cggaaaaacc
    49441 aattcccgcc tgcttcaacc atgcttcatc gacatggtcg gtccccgcag tggcagttcc
    49501 aacaaattta atgggttttc ctgccagcaa agattcattc actttcgtga ccgaacgcac
    49561 catcagcgca tccgcgtctg ccagttgagc gacggggatt ggacgcccgg gaaccgcgat
    49621 cacctcaccc aaacggctaa ataagtcgcg ggcataaggc atattttcat caacaaggat
    49681 tttcacgttt gtgttacctg tatgagacga gagttaaccg gacaagtatg ccataatctc
    49741 gcggccaggc atacttgcga agatttcagg tataaggata cgtaatgata caacctattt
    49801 ccggccctcc tcctgggcag ccaccaggtc agggagataa cctgccgtct ggcgcgggca
    49861 atcagccttt atccagtcag caacgtactt cgctggaaag cttaatgacg aaagtgacct
    49921 cactgacgca acaacaaaga gcagaactgt gggcgggtat caggcacgat attggtctgt
    49981 cgggagattc accgctgctt tcgcgtcact tccctgccgc tgagcataat ctggcgcaac
    50041 gtctgctggc cgcgcaaaaa agccattctg cccgccagct tttagcgcaa ttaggggagt
    50101 atttacgtct ggggaatcat cgtcaggcgg tcacggatta tatccgtcat aactttggtc
    50161 agacgccgct gaatcagctc tcaccggagc aattaaaaac cattctcacc ctgttgcagg
    50221 aagggaagat ggttattccg caaccacagc agcgcgaggc gaccgaccgt cctttattac
    50281 cggcggagca caatgcgcta aaacagctgg tgaccaaact tgcggcggca acgggggaac
    50341 ccagcaaaca gatctggcaa tcgatgctgg aactttccgg ggtgaaagat ggcgagttaa
    50401 ttccagcgaa actgtttaac catctggtga cctggctaca ggcgcgtcag acgctaagcc
    50461 agcaaaatac gccgacgctg gaatcactac agatggcgct aaaacaacct ttagatgcca
    50521 gtgaactggc ggcgttatcg gcatatatcc agcaaaaata tggcctttct gcgcaatcat
    50581 cgctttcttc tgcccaggcc gaggatattc ttaatcagct ttatcaacgg cgggttaaag
    50641 ggattgatcc gcgtgatatg caaccgctgc ttaatccttt tccaccgatg atggacacgt
    50701 tgcaaaatat ggcaacgcgt cccgcgctgt ggatactgtt agtcgcgatt atcctgatgc
    50761 tggtctggct ggttcgttaa ccccgacgaa atgacagtat cgtgacaata atacccagca
    50821 ccgcagagat cgccccggca agaaataccg aagagtaacc aaacgtggtc gccagcattc
    50881 ccgccagcgg cccggagacg ccgagggcga tatcctgaaa cgcggcgtaa ccgcccagtg
    50941 cggtgccgcg aacttgtgag gggacgcgtt taaccacctc cacgcccagc gcaggaaaga
    51001 taagcgaaca tccggctccg gttaacgccg cgcccgctaa tgcgacccat gcacctgggg
    51061 cttgccagag cagcaacaag cccaccgttt ctacaagcag agagacaatc gccactttca
    51121 cgccgccaaa acggtccggc atccagccaa acatgacgcg catcacgaca aatgcgccgc
    51181 caaacgcggt aagagtaaag cccgccatcg cccatccttt gctggcaaag tagagcgaaa
    51241 cgaaagtccc gataaccgca aaaccaacgc cttgtagtgc cagccctaac cctggtttcc
    51301 agataagccc gacaacgctc cacagcgatg gacgttctcc cgccagggcc ggtactttgc
    51361 gcactgtgcc gttacaggcc cacgccagta agggtaatac cattgtggtg atcgccagtg
    51421 cggcaaaacc gtaatggcta tgaatcaaca ggccaagcgg cgcaccaaca gcgagggcac
    51481 cgtaaatcgc cattccgttc catgacatca ctttgccaga gtgttttggc cctacgatgc
    51541 ctaaccccca ggtcagagcg cctgtcagta actggctttc accaaaccca agaatcaaac
    51601 gcccgatgac caacagggca aatttgaacg gtgcggagac aggcaaaatc gccgccagca
    51661 gcaacgcgcc gccagccaga ccacaagcta acatcccctg aagcgccgaa cgttttgcgc
    51721 catattgatc ggccagtcgc ccggcgtaac cacgcgtcag caccgtagcc agaaactgaa
    51781 tcccgacggc aatgccgacc atggtattgc catagcccag atcatgatga acaaacagcg
    51841 ggataaccgg caacggcaac cctacggtca tgtaggtgag aaaaaccgca aaagagatgc
    51901 ggaagagcga aaaactggca gaagatcgtg tttcggtttg gcttacagca gtcatgcatt
    51961 actccagaat gcagcgcaag gcgaggagta tccccgtctc atctctctgg tttcagggtt
    52021 acggtgcgtt ggcaggattt aacgcgtacg tcttttcaga aggaaatcga caaagcggga
    52081 agtttgccta gaactggtgg cgattgtcaa tgatgtgaaa aagggaacca tcaggttccc
    52141 ttttgcgtta gtgccggagg ccgcattggc gcgtaacgtc ggatgcgacg ctggcgcgtc
    52201 ttatccgacc tactccggcc tactgcgaat taatcttttc agcttgcgca ttaccagcgt
    52261 ggcgttggtg ccgccgaagc cgaagctgtt agacataaca gtggtcagtt cgcgatcggt
    52321 cgtttcggtc acgatgttca gacccgcagc ctgctcgtcc agctcttcaa tgttgatgct
    52381 cggggcgata aagccgtgtt ccagcatcag cagagagtag atagcttcct gcacacccgc
    52441 agcgcccagt gagtgaccgg tcatggcttt ggttgcagaa atcgccgggc tcttatcgcc
    52501 gaacacttca cggatagctg ccagctcttt cacgtcgcca accggagtcg aagtaccgtg
    52561 agagttcagg taatcgattg gggtatcaac gccatgcatc gccatctgca tacaacgtac
    52621 tgcgccttcg ccagacggag caaccatgtc tgcaccatca gaggttgcgc cgtagccaac
    52681 gatttcagca tagatgtaag caccacgcgc cagcgcgtgt tccagctctt caaccactac
    52741 cataccgccg ccgccagcga taacgaaacc gtcacggtga gcgtcgtaag tacgggaggc
    52801 tttttccggg gtgtcgttgt atttagtaga tagcgcaccc atcgcgtcga attcgcaggc
    52861 catttcccag cacagctctt cgccgccgcc agcaaacacg atgtcctgtt tgcccagttg
    52921 gatctgctct actgcgttac cgatacagtg tgcggaagtc gcacacgcgg agctgatgga
    52981 gtagttaacg ccatgaattt taaacggcgt ggcgagacag gcagaaacgc cggatgccat
    53041 cgctttagtg accacatacg ggccaaccgc tttcaggccg cgcgggccgc gcattgcgtc
    53101 agcgccgaac acctggaaac gcggggaacc gccgccggaa cctgcaatca ggccaacgcg
    53161 cgggttattc tggtaagctt ccggagagag gcccgcatca gcgattgcct gctccataga
    53221 aaggaatgca taaatggatg cgtcgctcat aaagcgcaca actttgcggt caatgaggcc
    53281 agtggtatcc agttttatgt tgccccagac gtggctacgc atgccggaat ccttcagctc
    53341 ctgagagaaa gtgatccctg aacgtccttc acgcagagat gccaggactt cctgctggtt
    53401 attaccgatg ctggaaacaa tgcccaggcc agtaatcact gcacgtttca ttcaatacct
    53461 ctgtaagtcg cacatagagt aagtttcgaa tgcacaatag cgtacacttg tacgccgaac
    53521 aagtccgatc agccatttaa tagagatatt tgcgcagcct tacacacatc gctaagatcg
    53581 agccaccgcc tgtaagacga gtaacttacg tgaaacacta ctccatacaa cctgccaacc
    53641 tcgaatttaa tgctgagggt acacctgttt cccgagattt tgacgatgtc tatttttcca
    53701 acgataacgg gctggaagag acgcgttatg tttttctggg aggtaaccaa ttagaggtac
    53761 gctttcctga gcatccacat cctctgtttg tgatagcaga gagcggcttc ggcaccggat
    53821 taaacttcct gacgctatgg aaggcatttg atcagtttcg cgaagcgcat ccgcaagcgc
    53881 aattacaacg cttacatttc attagttttg agaaatttcc cctcacccgt gcggatttag
    53941 ccttagcgca tcaacactgg ccggaactgg ctccgtgggc agaacaactt caggcgcagt
    54001 ggccaatgcc cttgcccggt tgccatcgtt tattgctcga tgaaagccgc gtgacgctgg
    54061 atttatggtt tggcgatatt aacgaactga ccagccaact ggacgattcg ctaaatcaaa
    54121 aagtagatgc ctggtttctg gacggctttg cgccagctaa aaacccggat atgtggacgc
    54181 aaaatctgtt taacgccatg gcaaggttgg cgcgtccagg cggcacgctg gcgacattta
    54241 cgtctgccgg ttttgtccgc cgcggtttgc aggaagccgg attcacgatg caaaaacgta
    54301 agggctttgg gcgcaaacgg gaaatgcttt gtggggtgat ggaacagaca ttaccactcc
    54361 cctgctccac gccgtggttt aatcgtacgg gcagcagcaa acgggaagta gcgattatcg
    54421 gcggtggtat tgccagcgcg ttgttgtcgc tggcgttatt acggcgcggc tggcaggtag
    54481 cgctttattg cgcggatgaa gcccccgcgc tgggtgcttc cggcaatcgc cagggggcgc
    54541 tgtatccgtt attaagcaaa cacgatgagg cgctaaaccg ctttttctct aatgcgttta
    54601 cttttgctcg tcggttttac gaccaattac ccgttaaatt tgatcatgac tggtgcggcg
    54661 tcacgcagtt aggctgggat gagaaaagcc agcataaaat cgcacagatg ttgtcaatgg
    54721 atttacccgc agaactggct gtagccgttg aggcaaatgc ggttgaacaa attacgggcg
    54781 ttgcgacaaa ttgcagcggc attacttatc cgcaaggtgg ttggctgtgc ccagcagaac
    54841 tgacccgtaa tgtgctggaa ctggcgcaac agcagggttt gcagatttat tatcaatatc
    54901 agttacagaa tttatcccgt aaggatgact gttggttgtt gaattttgca ggagatcagc
    54961 aagcaacaca cagcgtagtg gtactggcga acgggcatca aatcagccgt ttcagccaaa
    55021 cgtcgactct cccggtgtat tcggttgccg ggcaggtcag ccatattccg acaacgccgg
    55081 aactggcaga gctgaagcag gtgctgtgct atgacggtta tctcacgcca caaaatccgg
    55141 cgaatcaaca tcattgtatt ggtgccagtt atcatcgcgg cagcgaagat acggcgtaca
    55201 gtgaggacga tcagcagcag aatcgccagc ggttgattga ttgtttcccg caggcacagt
    55261 gggcaaaaga ggttgatgtc agtgataaag aggcgcgctg cggtgtgcgt tgtgccaccc
    55321 gcgatcatct gccaatggta ggcaatgttc ccgattatga ggcaacactc gtggaatatg
    55381 cgtcgttggc ggagcaaaaa gatgaggcgg taagcgcgcc ggtttttgac gatctcttta
    55441 tgtttgcggc tttaggttct cgcggtttgt gttctgcccc gctgtgtgcc gagattctgg
    55501 cggcgcagat gagcgacgaa ccgattccga tggatgccag tacgctggcg gcgttaaacc
    55561 cgaatcggtt atgggtgcgg aaattgttga agggtaaagc ggttaaggcg gggtaatctg
    55621 ctctcgcagt gtttgccgga tgcggcgtga acgccttatc caacctacgt gtgacgtgta
    55681 ggcctgataa gacgcgataa gcgtcgcatc aggcatggtg ctccagacgc cgcaacttac
    55741 agctgtgacg cctgctgaaa caggttttcc cacatatcgg tcaccagcgc ctggtcacgc
    55801 ggcgacaatt caccggcacc aatggctttt tccagactct ggctaacggt cgtatgcacc
    55861 gcctgagcgg agtggtcatc accactttcc agttctgcga tggctaatgt caggtggcca
    55921 cgcaaatacc cactggcaaa caactcatca tcactggcat ggtcaaccat gccgtcgatt
    55981 aatgccagaa tgcgtgattc aaactccgcg atcatcttct ttcctcattg taaaacgtgg
    56041 cgctggcttc agttgagcgc ttccggccac gggaactgtt ccgccgtaag ttccggcgtg
    56101 tgataataat tttgtagtgc tttaatgaag cgtgccgggc gttccggaat gccatttgtc
    56161 agataatcca tcacctgcgc atgtacccgc cgttgaaaca ccacacgatc cggttcgaaa
    56221 tccccttcca gattgtcgca actgacgtta aacggatatc cagccgccac gcagaataac
    56281 cagtcgaacg cctgcggctt cacttcaaca tcttcaaact ggctttgcgt ctgggcatcg
    56341 cgtccgtccg ggcaatacca gtagccgaaa tcaaccagct cacggcgcgc ttttccggca
    56401 atacaccagt gcgaaatctc atgaattgcg ctggcataaa agccatgagc aaagacgatt
    56461 cggttatacg gtacttccgc atcagcagga agatagatcg gttcgtcgtc gcctttaatc
    56521 agacgggtat taaattcatc ggcaaagcag ctattaaaaa tttcaatcaa ctgctcgtag
    56581 tggtgtgtac tgttcattag ttcatcccca accagtggag gatctcctgt ccgtggctat
    56641 cgtaaagtaa tttggcactc atcaccgccg agacgataac aatcatcggg cggatcagct
    56701 tttgtccttt gctcaacacc aaccgtgaac ccatgcgcgc gccgaggaac tgccccacca
    56761 gcatgacaaa gcccgtcgcc caaatcactt tgccgccgag aataaacagc agcagaccgc
    56821 caatgtttga cgttgcgttg agtaatttgg cgtgagccgt ggctttggcg aggttgaatc
    56881 cgcacagcat aacgaaggcc agcgcgtaaa acgatccggc agccgggcca aagaatccgt
    56941 cataaaaacc gacgcagcca ccggcaatta acgcgaaggg taaaccgtac atccggcgct
    57001 ggcgatcttc ttcacccagc tttggcatca gcaagaaata gaggccgata caaatcacca
    57061 gaatgggcaa aatctgccgt aagacatcag cctgaacgta ctgtaccaac agtgcgccgc
    57121 tcattgagcc gacaaaggtc atggcaatat tgagtttctg atcgcttaaa ctaaccactt
    57181 tgcggcgaat aaagtagata gtggcggaaa tagagccgcc gcaggcttgc agtttattgg
    57241 ttgccagcgc gttagcggga gacattcccg cagccatcaa tgccggaatg gtgagtaacc
    57301 caccgccacc ggcaatcgag tcgataaatc ccgccagcat ggcgacaaaa aagagaactc
    57361 ccagcaacag cggagaaacc ataaacaggc tattaaacgt ttccattaga tcacgtgctc
    57421 atccagtagc gcctggcagg aaggcggcaa cggaggcggt gtcttcttct caggctttgt
    57481 tgttcccggt tttggaggtt caaaccagct ttgcagttct gccccgcaac catcgcctga
    57541 tggcggtaaa ggttgatctt cacactccag actatcggca gggcaacgta atcgtacatg
    57601 catatgcgcg cgatgctgga accagggtcg cactttgcgc aaccagtcgc gatcggtgcc
    57661 cgcatcaagg caaagttgtt gtttaatcgc cggattaaca aaaatgcgcg tgacgtcttt
    57721 gtcctgggcg gcgagtttga tcaagctgaa aatttctggc ttccacagcg tggagacaac
    57781 gtgtttaccg tcgcgggaaa ctaagtccag tgcttgcggg cgcaagagct gcgcggaggt
    57841 ccagcgagtt ttcggcagtt gcagaaagat atcgacatcc agtccggtct ggtggctggc
    57901 atgaccgccg ttgaaacgcc caccagcggg catccccata tcgccaatca gcaccgtacc
    57961 catgcccaga ttgctcacct ggctactcag acgctggata aacatcacca gatccgggtg
    58021 accgaaatag cgacgctgat cggtacgcat gacctgataa tgttcggact gtatcggcaa
    58081 cgtgtcagcg ccgacaatac agccattaga aaaactgcct atcgattgtg cgctacccgg
    58141 cacaggttgg gttatttttt gccacggcgt cgctgccagg ctagcactac tggcaagcag
    58201 agccagcagc gcaatcgcgg ttttgttcat tttttaccag cgtggaatat cagtcttcac
    58261 atcggcattt tgcgcccgct gccgtaacag gtgatccatt aaaacgatcg ccagcatcgc
    58321 ttcagcgatc ggcactgcac ggatcccgac acagggatcg tgacggcctt tggtgatcat
    58381 ctcaacttct tcgccaaagc ggttaatagt gcgccccggc acggtaatgc tggaggtcgg
    58441 tttcagcgcc atatgagcaa tgatttgctg cccgctgctg ataccgccga aaatgccgcc
    58501 cgcatggttg ctctggaaac cgtctttggt gatttcgtcg cggttctggc tgccgcgcag
    58561 cgccactacg tcaaaaccgt cgccaatttc cacgcctttc accgcgttga tgctcatcag
    58621 cgcatgggcg atgtcggcat ccaggcggtc aaagaccggc tcgccaagtc cggcggggac
    58681 accactggca acaacggtga ctttcgcgcc gatggagtcg ccctcttttt tcagcgcgcg
    58741 catcagttca tctaacgcgt cgattttgtc ggggtccggg cagaagaacg gattttgttc
    58801 aaccagcgac cagtctttga tttccagcgg aatgtcgccc atctgggtca ggcagccgcg
    58861 gatttcaata ccaaatttct cggcgagata ttttttggca atcgcccctg ccgccacgcg
    58921 catggcggtt tcgcgggcgg aagaacgtcc accgccgcga taatcgcgca gaccgtattt
    58981 ttgttcgtag gtgtaatcag catggcctgg acggaagacg tctttaattg cgctgtaatc
    59041 ctgagaacgc tggtcggtat tttcgatcaa caagccaatg ctggtgccag tagtaacgcc
    59101 ttcaaaaaca ccggagagta ttttgacctg atccggctcg cggcgctggg tggtatagcg
    59161 cgatgtccca gggcgacgac gatcaaggtc atgttgcagg tccgcttccg tcagcggaat
    59221 gcctggcgga acaccatcga cgatgcagcc gagcgccagc ccgtgcgatt cgccgaaggt
    59281 ggttacgcga aagagttgtc caattgtgtt tccagccatc acggctccgt tattgttgtg
    59341 tttgcgtgtt tacttaatct ttataaatcg cgaaatgttc tcgtgcggca ataagctgct
    59401 ctttagtgag cataaacaca ccatcgccgc cgttatcaaa ctccagccag gtgaacggaa
    59461 catcgggata ttgttccata agatgtacca tgctgttgcc cacttcacaa atcaacacgc
    59521 catcatcagc aaggtaatct gccgcgttac cgagaatgcg acgcgtcagt ttcaggccgt
    59581 cggtgccaga tgccaggccc agttccggct cgtgacggta ttcgtttggc aggtcggaca
    59641 tatcttccgc gtcgacatac ggcgggttag tgacaatcag atcgtattgc actttcggca
    59701 aatcacggaa cagatcggaa cgaatcggaa tgacgttgtg gatcagaccg tgttcttcga
    59761 tgttctgttc ggcaaccgcc agcgcgtcgg gagagatatc taccgcgtcg acttccgctt
    59821 ccgggaaggc ataagcacag gcaatggcga tgcagccgct accggtacac atatctaaaa
    59881 tatgctgcgg ttgcttgctg atgagtccgg caaatttatt gttgatcagt tcaccaatcg
    59941 gcgagcgcgg caccagcacg cgttcatcga cgtaaaattc atgaccgcag aaccaggctt
    60001 tgttggtcag gtaagccacc ggaatgcgct cgttgacgcg gcggatcacg cgttcaacaa
    60061 tacggtgttt ttcgctggag gtcagacgcg cggtgcgcat atcttccggg atatccagcg
    60121 gcaggtagag cgatggtaac accagctgta cggcttcatc ccacgggtta tcggtgccgt
    60181 gaccgtacca gatatttgcc gcgctgaagc ggcttaccga ccagcgcaat atgtcctgaa
    60241 tggtttgcag ctcatttact gcttcatcaa cgaaaatttt atccacgtat tcctccaggg
    60301 catgatcgca ataatttcgg cggctagttt gccatgaaga tgacgataaa tcagcattca
    60361 cgcgcggtga gtgaggaaaa atacgtttaa aacgatcgat tgtgctacga gtcgggtaaa
    60421 ctgtaggaaa attagaaata gagacagata aatgaaaaag aaaacaacac ttagcgagga
    60481 ggaccaggct ctgtttcgcc agttgatggc ggggactcgc aagattaagc aggacacgat
    60541 tgtccaccga ccgcagcgta aaaaaatcag cgaagtgccg gtgaaacgct tgatccagga
    60601 gcaggctgat gccagccatt atttctccga tgagtttcag ccgttattaa ataccgaagg
    60661 tccggtgaaa tatgttcgcc cggatgtcag ccattttgag gcgaagaaac tgcgccgcgg
    60721 cgattattca ccggaattgt ttttggattt acacggtctg acgcagctgc aggccaagca
    60781 ggaactgggc gcgttgattg ccgcctgtcg ccgcgaacat gtgttttgtg cctgcgtgat
    60841 gcatgggcac gggaagcata ttttgaagca acaaacaccg ctgtggctgg cacaacatcc
    60901 gcatgtgatg gcatttcacc aggcaccgaa agagtacggc ggtgatgcgg ctttgttggt
    60961 gttgattgaa gtggaagagt ggttgccgcc ggagttgccc tgaatgctgg cagcaaatgc
    61021 cggagcagcg taaacgcctt atccggccta caaatatgtg ataattcaat aaattgcaat
    61081 tcacttcgta ggcctgataa gacgcatcag agcaatacta cgaacttcag ttcattcaat
    61141 acatactgaa aacgacacgt cgaatcaatc ggttgcccac tactggtatt cacattcacc
    61201 acaatatcgt gtagtacttt atccgttacc ggatgacgag ttggcgtaac cgttaccgtt
    61261 gatttatcac aaccgtaaga ggtaaaggtt aacaattctg ataccgcgcg gcagtatgct
    61321 cctctgccat cattatcaga aaaattactg ccatcacaga gtctgccgcc ttccatcagg
    61381 taggtcgaaa gcgtggtggt cactgttgac cccgtggact gaattcgtaa cgccttatta
    61441 tggctgacat aattttcact ccaggagata tcgtatgaag cgaccggtat cgttgggcat
    61501 attgtgttat gcactgtagg ggccgtatag cttccaaatc cgggtaaatc tgtataggaa
    61561 taaaaggatg cgatccaagg gcaaccataa tcatcagatg caacggtaat tttaaacgtc
    61621 atatatgcac aacgaaaaat tttcgtatct atgccagggc aatttgagtt attaattgta
    61681 tcaaagtcag tcagtaccaa agttggagct gaacccgacc cagaccagga aataacccgc
    61741 aacgtatcgc ctttatacgc aaaagaatca tatttcccat atgcaacctc ctttggcgtt
    61801 atcaccgtaa cattgaaaac actgctcgct acagcaccgg ttaccttgtc catggcataa
    61861 taaactctca cactgtcaaa actcgcactc ataagtttat ctccagcata actcttagat
    61921 gcgccgacaa cactcagaac gagcagcatt aggctcgcga aaattatggt ccattttttc
    61981 atttcagaaa tactcaaagg ttgccgtcac tgtggcacta aagtctccgg acgttacatc
    62041 tcgcccgctc tttgcctgta accaggtatt aaaatttagg ctgttatttc cctgattcag
    62101 cgggagcgtg acgcctgttg tattgttgat tggcaccgat gtgcctgccg cagtttcaat
    62161 accaatccca gcgccggatg ctgatgacga ggagtcaaag gccagaaagc cgggtagagc
    62221 actatcttct gttcctgtca acgttacttt gacattaaac aatgtagaac tatgacaatc
    62281 tttcaattga aacaccaatg gcactcgctc tgactgccca aggttcatta aatcactggc
    62341 ggcaatggca ggaaaatgta cttctgccaa ctggctacca ttaatcactg gcgtacacga
    62401 tttactgatt aaattgccgg taaagtgcaa attatctcct gctattgtgg ttccactgcc
    62461 cagcaacatc aggctactaa taaaaaacaa cgtacgcttt ttcttcattg gtaatctacc
    62521 tgtaacgtgg cccaggcctc aaagtccgcc tctggcagaa tgacaccact cttttttact
    62581 ggcacagccg ttaacaccgg taaatcggtt tcattcacca caagtggcgt attgatcgta
    62641 aaactctgcc ctgcctgttt gagctgaatg cctaaaccag tgatattgct gcttactgca
    62701 gcattatcaa aatcgcttgg tgtgccactc cagctcaagg tcatttccat taatgggtcg
    62761 cgaaccgccg tatcgcaggt aatggaatac ggcacggttt gctcataatt gctgccatta
    62821 atgtcatcaa taagcacatc ctgaaaagtt atttcaatgg tttgagcgtt gctaatcgtg
    62881 cagttaggcg gctgcactaa ataaccgtga aaagagacgt ttgagagtgc catcgagcaa
    62941 aagccccaca gcaaaataag cgagatccgt ttcattggta atccaccaca attgtggcac
    63001 tggcgttaaa ttcagctgcc gctaacgttg taccactctg tttcaccggc acagcttcca
    63061 gaacgggaac tcccgaggaa aaattaaatg gcaaccatga gccagaggtt aagtcgagta
    63121 ttgtgtgatc actacttttc tgaacccgaa taccaaaccc aggaataccc gtgctgataa
    63181 ctgtttcacc gttaatcacg accgttgtcg cctgcatctg tagacgcagg tcatccattg
    63241 ccagattgtt acaaacaagc gagtagtcaa tgggacgttt ataatcaacc ctattgatct
    63301 tattaatgaa tacattacca aactcaactg ccgcaccatt taccgtacaa ggaggagggg
    63361 catcgacaag cacccgtaaa ttcatactta ctgacttact cgctgcaact gcaaagccgc
    63421 taccaacaca acatatcagt aacccagtta ccgcccagac agaacaagat cctttcatac
    63481 tggctctccc ggcctcagcc tttgcggctc tgctcttcat cgacctagca actgttaccg
    63541 ttgcaagtga aaaagagcgg catccgagcg ccatagtcgt tgacataggt cagtaccggc
    63601 acgctaccca tcttcacatt cagcggtgca gaggttttcg gcgacatcac tattggcgag
    63661 aaaccggctg ccggattgcc atttttctgt gtgctggcat tactgaaaat gacgtaatac
    63721 gacgtcgggt tattgacggt aaattcctga ccgttgcggg ttaaagtaat cttatattgc
    63781 taggggtttt tcatgctgac gttctccagc gccttcggtc gccagaacaa cttaatacgc
    63841 gtttgtagtg ctatttgcag cgtgttggct ttgtttgatt ttggcgggat ctcacgcacg
    63901 ttgaagtaga acaggctttc acgatcggca ggcaatttgt tgatatccgg cattccctgc
    63961 acttttacct gcccgttcat cattgaatcg atgcgttgca ccggtggcag aacagttaac
    64021 ggtgaactga tcttgttacc cttttcatct tcgatccagc tttgggccag ataaggcagt
    64081 ttcggatcgt tattgcgcaa cgtcacgctg attgatttgt cgctttcatt aaatatcaaa
    64141 cgggtacgat ctggcgttac agaagcctgg gcagacaaac tggcagcgct aagcagtaac
    64201 gctagcgtcg tagcacccaa tttggcagag caaagtaaat cagacatgga tcattcctta
    64261 atcacgttat tggttagctg aaacagaaac agggggattg gtgggtgtca cctgttgcgt
    64321 ctgctcctga atgacaggct ttatgtcatc aggaacaacc ggcgcgacgt tgcctttatg
    64381 ctggcatggc agtaacaagc cgttaaacag atcggcaggc agcgggtccg gcaggttgat
    64441 atcgcaatgc gcaacaccac tccagaacac actcatgtgt tcgccaggtt tcactccagc
    64501 cagataaaca ttgccgtcat catcgacaag gcccactgtc tgctcgttat cattttttac
    64561 ttctgcgcca aacggaggat ggctgccgtc ttgtaaacgc agcaccgcca tcgctttttg
    64621 accactgatg acggcaaatt tgcggtagcc aatcgcccct tcagttagcg tggcttgtac
    64681 caccgactgg gttgcttcgg cgttttccgg cagtttgttg aggtcgatat acgcctgatt
    64741 gcggtaatag ttattgacgt cagaaacgac ggctttacca aacatattgg tataaacagc
    64801 agcaccgtta ccttctaccg gaacatcggc aacgccatcg gcatcaatca acaagcgtgt
    64861 cccgcccata ttctgggtac ggtgaagtgc gccaccgtgg gcagtaagcg ttgcgccgcc
    64921 ctgtaacgac aagcccgcag aggtgtattg cccttcatgg tagttcgcac tgaggtcaac
    64981 ctgcgccagc gaaccatcat ggctgtaata gccgtcaacg ctggtgtgtt tgtcactggt
    65041 gccgacgttc aactgatagt gagtcgcgtc atcgacacgg ctgaaataac cgacctgact
    65101 gctgtccgtc ccactgccat agtaccgtta tagctaacgg tactgttgtc gccccacggc
    65161 atactgagcg aaatgtacat gcctttgtcg gcctggttgt catactcgta acggtagcca
    65221 gtcatcgaga tgctgacatt acgaatactg cccatattga aatagtgcga gagcatgatg
    65281 ttgtagtttg tctgctcctc gcgatcccag taggtatggc gggtatagtt gagataaacc
    65341 gaaacacccg catcgcggaa gttctggtta taagtggcgg tgtacatctc tttgtcgttg
    65401 cccgtgcgta ccattccgct gtcactggca tccagatact cgctcatggt cataaagttc
    65461 tcttccgaga agcgatatcc agcaaaagta acgcggctgt tgagctggtc aaagtctttg
    65521 gaataactca cacggaagga gttaccgtcc agcgaacctt tgccataagc ggtgtcttta
    65581 tccagtttgg tgtgcgagtg agtaacatca aacgcgaccg cgccgaatgt agacaaatcg
    65641 cgaccgacac caagcgccgc agactgatag ttttcatctc ccagtgcgcc gccatataac
    65701 gaccagccgt tagcgatccc ccaggaagct tcagcaccag aaaaaaattc accctcgaca
    65761 tggtgtcccc actcttgcgg acggcccatc atgatcttat agcgaacctg acctggacga
    65821 gtgaggtatg gcatcgaggc ggtgctgatg tcatattcct gcacctggcc gttctgttct
    65881 tcaatgcgaa tatgcaacgt accggagaca gaatcaccaa gatcctgaat acgaaacggt
    65941 ccagccggaa cctgcgtttc gtaaatcaca cgccccatct ggctgacggt cacttttgct
    66001 gtggtgtgtg ccacgccgga aatgtctggc gcgtagccgc gcagattggg aggcaacatt
    66061 tgatcgtcag tactgacact gccaccaaca tagttaaaac catcaaaaat atcggatctg
    66121 aggtaatcct cgcccagcgc cagtttggct tttaatgatg gtaacgcccg cctggcatag
    66181 tagcgactcc actcccattt ttttgagttt catcgccgct gaattcatcg tcatcattac
    66241 tgcgagtatg ttgatagtta gtctgccagt cagcacgcat acgccacggc cctaggttaa
    66301 ccccaaccgt cccgttgccg ctgatctcgt tactatcatc accgccattt tcttcgtgcc
    66361 gggtttgtgc gttgatgctg tagtccgcga cgatcccgga gatgccgtca tcccaacgtg
    66421 aaggcggatc cctgtcggga taagtatatt cgaggtaagc ctgtggcagt gaaatgacta
    66481 atgcggactg gcttaaatca gccttaattt ccatgccttc cagttgaccg gatttcaggc
    66541 atttagcatc gtggctccat tgcagatttt tcgccacgtc ttcttttaaa ccaaactgcg
    66601 ctaccagttc cggtgtcaga caagcatagc ttttgctcgc gtcatcttca ccagcatacc
    66661 agtaaatatc gtactcttcc gccaatggct gtttatttag ttgaacctgt aaattgtatt
    66721 ttccgggctc aacatatccc tggctggaaa aacgcttcat atcaattttt gtgtcgcctt
    66781 ttaattccag aaaacgggaa tcaaactgaa tgtcgtcttc agcccagaca ctgctataac
    66841 tccctgacat tgccagcgca atgcaccacg gaagaatccg taatcgaaaa agagaatggt
    66901 caggcataca cgtttctatc cgtttttatt cttgttcggt caaaatacat ttaaatcccc
    66961 catagcagtg tcgctatggg ggtatgcctt acaggtaagt aatctggaag gtggtatttg
    67021 cttcaaaatt acccagatct ggcgcatcag ctgcgccgac cagccaggcg ttaaagttca
    67081 gtgtctgctt cgctttgcct ttgttggtag aagtgtcttt cactattttc tggtcaatac
    67141 ccatgccgct tttgtaagag gtacccagag agtcaccgat cgccaggcta acattgttaa
    67201 atgccgcacc ggtatcggta ttgaaaatgg tgtaataatt gccgctattt gcagaagaaa
    67261 cggtaccagt aaaggtagtg gtcattgttt cctgagtatc aaatacgcaa tcttgcagac
    67321 gaatctggaa acttttcggc gtggtggtgc cgttattttt cagtttttcg gcaccgatat
    67381 cacccatatc aacttccagt ttgtgatcgt ccggaacgat agaacacact gagtcttcaa
    67441 tggtgccgta aaaacgtgca gtaccgttgt tacccgcagc gattgttgcc gtagaggtga
    67501 acacgcccat caccattgcc gcagcaatag ctgttttaac aaacttactc attatttgtc
    67561 cttaaactat aaataattaa aaatataaac attgcaatac attgattcag tcaatagcca
    67621 atgtttatac ccagccattg aatacggcat gcgacccaaa aagaacaggt cgtagaaaat
    67681 tgtattcatt ggtgtggggc cgcatagtga tgtttccaca cccctgatcc aatttaattt
    67741 tactaaagag taaatgtaga tttaattaat atattgacag aagaaaggtt atcttaagga
//
LOCUS       DAZXUL010000004        65862 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZXUL010000004 DAZXUL010000000
VERSION     DAZXUL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65862
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.004"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..437)
                     /gene="folK"
                     /locus_tag="ACH3L1_000219"
     CDS             complement(<1..437)
                     /gene="folK"
                     /locus_tag="ACH3L1_000219"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFT4075143.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLR"
     gene            complement(434..1831)
                     /gene="pcnB"
                     /locus_tag="ACH3L1_000220"
     CDS             complement(434..1831)
                     /gene="pcnB"
                     /locus_tag="ACH3L1_000220"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFT4075144.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(1891..2787)
                     /gene="gluQRS"
                     /locus_tag="ACH3L1_000221"
     CDS             complement(1891..2787)
                     /gene="gluQRS"
                     /locus_tag="ACH3L1_000221"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFT4075145.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(2854..3309)
                     /gene="dksA"
                     /locus_tag="ACH3L1_000222"
     CDS             complement(2854..3309)
                     /gene="dksA"
                     /locus_tag="ACH3L1_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFT4075146.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3487..4191)
                     /gene="sfsA"
                     /locus_tag="ACH3L1_000223"
     CDS             complement(3487..4191)
                     /gene="sfsA"
                     /locus_tag="ACH3L1_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFT4075147.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4206..4736)
                     /gene="thpR"
                     /locus_tag="ACH3L1_000224"
     CDS             complement(4206..4736)
                     /gene="thpR"
                     /locus_tag="ACH3L1_000224"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFT4075148.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4810..7239
                     /gene="hrpB"
                     /locus_tag="ACH3L1_000225"
     CDS             4810..7239
                     /gene="hrpB"
                     /locus_tag="ACH3L1_000225"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFT4075149.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7435..9969
                     /gene="mrcB"
                     /locus_tag="ACH3L1_000226"
     CDS             7435..9969
                     /gene="mrcB"
                     /locus_tag="ACH3L1_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFT4075150.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQVAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10189..12432
                     /gene="fhuA"
                     /locus_tag="ACH3L1_000227"
     CDS             10189..12432
                     /gene="fhuA"
                     /locus_tag="ACH3L1_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFT4075151.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12483..13280
                     /gene="fhuC"
                     /locus_tag="ACH3L1_000228"
     CDS             12483..13280
                     /gene="fhuC"
                     /locus_tag="ACH3L1_000228"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFT4075152.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13280..14170
                     /gene="fhuD"
                     /locus_tag="ACH3L1_000229"
     CDS             13280..14170
                     /gene="fhuD"
                     /locus_tag="ACH3L1_000229"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFT4075153.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14167..16149
                     /gene="fhuB"
                     /locus_tag="ACH3L1_000230"
     CDS             14167..16149
                     /gene="fhuB"
                     /locus_tag="ACH3L1_000230"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFT4075154.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16184..17464)
                     /gene="hemL"
                     /locus_tag="ACH3L1_000231"
     CDS             complement(16184..17464)
                     /gene="hemL"
                     /locus_tag="ACH3L1_000231"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFT4075155.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17630..17677
                     /gene="yadX"
                     /locus_tag="ACH3L1_000232"
     CDS             17630..17677
                     /gene="yadX"
                     /locus_tag="ACH3L1_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFT4075156.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17689..19110
                     /gene="clcA"
                     /locus_tag="ACH3L1_000233"
     CDS             17689..19110
                     /gene="clcA"
                     /locus_tag="ACH3L1_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFT4075157.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19134..19199
                     /gene="yadW"
                     /locus_tag="ACH3L1_000234"
     CDS             19134..19199
                     /gene="yadW"
                     /locus_tag="ACH3L1_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFT4075158.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19192..19536
                     /gene="erpA"
                     /locus_tag="ACH3L1_000235"
     CDS             19192..19536
                     /gene="erpA"
                     /locus_tag="ACH3L1_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFT4075159.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19583..20206)
                     /locus_tag="ACH3L1_000236"
     CDS             complement(19583..20206)
                     /locus_tag="ACH3L1_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFT4075160.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20244..21044)
                     /gene="btuF"
                     /locus_tag="ACH3L1_000237"
     CDS             complement(20244..21044)
                     /gene="btuF"
                     /locus_tag="ACH3L1_000237"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFT4075161.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21037..21735)
                     /gene="mtnN"
                     /locus_tag="ACH3L1_000238"
     CDS             complement(21037..21735)
                     /gene="mtnN"
                     /locus_tag="ACH3L1_000238"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFT4075162.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21819..23336
                     /gene="dgt"
                     /locus_tag="ACH3L1_000239"
     CDS             21819..23336
                     /gene="dgt"
                     /locus_tag="ACH3L1_000239"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFT4075163.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23466..24890
                     /gene="degP"
                     /locus_tag="ACH3L1_000240"
     CDS             23466..24890
                     /gene="degP"
                     /locus_tag="ACH3L1_000240"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFT4075164.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25045..26201
                     /gene="cdaR"
                     /locus_tag="ACH3L1_000241"
                     /pseudo
     CDS             25045..26201
                     /gene="cdaR"
                     /locus_tag="ACH3L1_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26290..26676)
                     /locus_tag="ACH3L1_000242"
     CDS             complement(26290..26676)
                     /locus_tag="ACH3L1_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFT4075165.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26990..27814)
                     /gene="dapD"
                     /locus_tag="ACH3L1_000243"
     CDS             complement(26990..27814)
                     /gene="dapD"
                     /locus_tag="ACH3L1_000243"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFT4075166.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27845..30517)
                     /gene="glnD"
                     /locus_tag="ACH3L1_000244"
     CDS             complement(27845..30517)
                     /gene="glnD"
                     /locus_tag="ACH3L1_000244"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFT4075167.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30579..31373)
                     /gene="map"
                     /locus_tag="ACH3L1_000245"
     CDS             complement(30579..31373)
                     /gene="map"
                     /locus_tag="ACH3L1_000245"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFT4075168.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31741..32466
                     /gene="rpsB"
                     /locus_tag="ACH3L1_000246"
     CDS             31741..32466
                     /gene="rpsB"
                     /locus_tag="ACH3L1_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFT4075169.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32724..33575
                     /gene="tsf"
                     /locus_tag="ACH3L1_000247"
     CDS             32724..33575
                     /gene="tsf"
                     /locus_tag="ACH3L1_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFT4075170.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33722..34447
                     /gene="pyrH"
                     /locus_tag="ACH3L1_000248"
     CDS             33722..34447
                     /gene="pyrH"
                     /locus_tag="ACH3L1_000248"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFT4075171.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34739..35296
                     /gene="frr"
                     /locus_tag="ACH3L1_000249"
     CDS             34739..35296
                     /gene="frr"
                     /locus_tag="ACH3L1_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFT4075172.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35388..36584
                     /gene="ispC"
                     /locus_tag="ACH3L1_000250"
     CDS             35388..36584
                     /gene="ispC"
                     /locus_tag="ACH3L1_000250"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFT4075173.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36773..37531
                     /gene="ispU"
                     /locus_tag="ACH3L1_000251"
                     /gene_synonym="uppS"
     CDS             36773..37531
                     /gene="ispU"
                     /locus_tag="ACH3L1_000251"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFT4075174.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37544..38401
                     /gene="cdsA"
                     /locus_tag="ACH3L1_000252"
     CDS             37544..38401
                     /gene="cdsA"
                     /locus_tag="ACH3L1_000252"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFT4075175.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38413..39765
                     /gene="rseP"
                     /locus_tag="ACH3L1_000253"
     CDS             38413..39765
                     /gene="rseP"
                     /locus_tag="ACH3L1_000253"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFT4075176.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39795..42227
                     /gene="bamA"
                     /locus_tag="ACH3L1_000254"
     CDS             39795..42227
                     /gene="bamA"
                     /locus_tag="ACH3L1_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFT4075177.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42349..42834
                     /gene="skp"
                     /locus_tag="ACH3L1_000255"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42349..42834
                     /gene="skp"
                     /locus_tag="ACH3L1_000255"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFT4075178.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42838..43863
                     /gene="lpxD"
                     /locus_tag="ACH3L1_000256"
     CDS             42838..43863
                     /gene="lpxD"
                     /locus_tag="ACH3L1_000256"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFT4075179.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43968..44423
                     /gene="fabZ"
                     /locus_tag="ACH3L1_000257"
     CDS             43968..44423
                     /gene="fabZ"
                     /locus_tag="ACH3L1_000257"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFT4075180.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44427..45215
                     /gene="lpxA"
                     /locus_tag="ACH3L1_000258"
     CDS             44427..45215
                     /gene="lpxA"
                     /locus_tag="ACH3L1_000258"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFT4075181.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45215..46363
                     /gene="lpxB"
                     /locus_tag="ACH3L1_000259"
     CDS             45215..46363
                     /gene="lpxB"
                     /locus_tag="ACH3L1_000259"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFT4075182.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46360..46956
                     /gene="rnhB"
                     /locus_tag="ACH3L1_000260"
     CDS             46360..46956
                     /gene="rnhB"
                     /locus_tag="ACH3L1_000260"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFT4075183.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            46993..50475
                     /gene="dnaE"
                     /locus_tag="ACH3L1_000261"
     CDS             46993..50475
                     /gene="dnaE"
                     /locus_tag="ACH3L1_000261"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFT4075184.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50488..51447
                     /gene="accA"
                     /locus_tag="ACH3L1_000262"
     CDS             50488..51447
                     /gene="accA"
                     /locus_tag="ACH3L1_000262"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFT4075185.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51546..53687
                     /gene="ldcC"
                     /locus_tag="ACH3L1_000263"
     CDS             51546..53687
                     /gene="ldcC"
                     /locus_tag="ACH3L1_000263"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFT4075186.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53744..54133
                     /locus_tag="ACH3L1_000264"
     CDS             53744..54133
                     /locus_tag="ACH3L1_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFT4075187.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54198..55496
                     /gene="tilS"
                     /locus_tag="ACH3L1_000265"
     CDS             54198..55496
                     /gene="tilS"
                     /locus_tag="ACH3L1_000265"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFT4075188.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55545..55805)
                     /gene="rof"
                     /locus_tag="ACH3L1_000266"
     CDS             complement(55545..55805)
                     /gene="rof"
                     /locus_tag="ACH3L1_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFT4075189.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55792..55992)
                     /locus_tag="ACH3L1_000267"
     CDS             complement(55792..55992)
                     /locus_tag="ACH3L1_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFT4075190.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56158..56703
                     /locus_tag="ACH3L1_000268"
     CDS             56158..56703
                     /locus_tag="ACH3L1_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFT4075191.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56700..57122
                     /gene="arfB"
                     /locus_tag="ACH3L1_000269"
     CDS             56700..57122
                     /gene="arfB"
                     /locus_tag="ACH3L1_000269"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFT4075192.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57136..57846
                     /gene="nlpE"
                     /locus_tag="ACH3L1_000270"
                     /gene_synonym="cutF"
     CDS             57136..57846
                     /gene="nlpE"
                     /locus_tag="ACH3L1_000270"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFT4075193.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58001..58825)
                     /locus_tag="ACH3L1_000271"
     CDS             complement(58001..58825)
                     /locus_tag="ACH3L1_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFT4075194.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58878..60596)
                     /gene="proS"
                     /locus_tag="ACH3L1_000272"
     CDS             complement(58878..60596)
                     /gene="proS"
                     /locus_tag="ACH3L1_000272"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFT4075195.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60707..61414)
                     /gene="tsaA"
                     /locus_tag="ACH3L1_000273"
     CDS             complement(60707..61414)
                     /gene="tsaA"
                     /locus_tag="ACH3L1_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFT4075196.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61411..61815)
                     /gene="rcsF"
                     /locus_tag="ACH3L1_000274"
     CDS             complement(61411..61815)
                     /gene="rcsF"
                     /locus_tag="ACH3L1_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFT4075197.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61933..62748)
                     /gene="metQ"
                     /locus_tag="ACH3L1_000275"
     CDS             complement(61933..62748)
                     /gene="metQ"
                     /locus_tag="ACH3L1_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFT4075198.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62788..63441)
                     /locus_tag="ACH3L1_000276"
     CDS             complement(62788..63441)
                     /locus_tag="ACH3L1_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFT4075199.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63434..64465)
                     /gene="metN"
                     /locus_tag="ACH3L1_000277"
     CDS             complement(63434..64465)
                     /gene="metN"
                     /locus_tag="ACH3L1_000277"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFT4075200.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64653..65228
                     /gene="gmhB"
                     /locus_tag="ACH3L1_000278"
     CDS             64653..65228
                     /gene="gmhB"
                     /locus_tag="ACH3L1_000278"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFT4075201.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65592..>65862
                     /locus_tag="ACH3L1_000279"
     rRNA            65592..>65862
                     /locus_tag="ACH3L1_000279"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15568 a        16331 c        18169 g        15794 t
ORIGIN      
        1 tcacgcaacg tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac
       61 agcataaatc cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc
      121 acttcattac caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct
      181 ttacgaacac gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg
      241 gcgggcgaag tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc
      301 agcggtgggg tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct
      361 aatgctttca gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca
      421 atatacgcca ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag
      481 tacgacgacg cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg
      541 gtgcggaaac ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt
      601 caacttcagc tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca
      661 gtttccatgc gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc
      721 gggttaacgt cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt
      781 cgttcatcgc cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct
      841 gtgccgtctc cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc
      901 gcatatcgtt atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca
      961 tcgggctgtc gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca
     1021 gatgatattc acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa
     1081 gcgattcttc aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga
     1141 tcggttctgc ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc
     1201 gcagcatacg taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa
     1261 cgccgtcctt cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc
     1321 tgtaatacag gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga
     1381 aaatgttgtc gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga
     1441 cgttaccttc gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta
     1501 catgagccag acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct
     1561 gctcaggcgt ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt
     1621 cgcgcacgcc gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta
     1681 ccttcagggc attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca
     1741 ccgtcacctg tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct
     1801 tgcggcaaaa attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt
     1861 caggacaaaa aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt
     1921 tacaattgcc gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat
     1981 ttgctcgacg ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc
     2041 cgcgattagt accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga
     2101 aagtttagcg ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca
     2161 gccaaaaagc tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg
     2221 cactatttct gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa
     2281 caacccatca cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat
     2341 aatgcctcgc agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc
     2401 ggcgttgtct ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct
     2461 ttgaatacgc gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc
     2521 gagtgcttca cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg
     2581 cagaccgtaa tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg
     2641 cgggtcgata tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata
     2701 gctgccgagc gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa
     2761 gcggccaata tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc
     2821 ccgcctgtgg taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat
     2881 ttcagccagc gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg
     2941 aataccaatt tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt
     3001 cagcgtcttc tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag
     3061 gctgaactct tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc
     3121 ctgcatatgt gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag
     3181 aatacgacgg aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg
     3241 atatggttcc accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc
     3301 ttcttgcatg ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg
     3361 aggccgctat aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac
     3421 aactgtgtga ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt
     3481 actttactac aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc
     3541 cgctttgtaa gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta
     3601 tttctcatcg atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa
     3661 aaaaataacc gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg
     3721 acctcgttca gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga
     3781 tttcacttca atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct
     3841 gcgttcggag ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat
     3901 tgattcatta aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat
     3961 aaatgcgccg ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt
     4021 gtctgaagtc gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt
     4081 attcgggcag tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg
     4141 tttgtaacgc tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat
     4201 ccttgttatt gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca
     4261 aacgaggagg cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc
     4321 gggaatgtca ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga
     4381 ttgctttgaa aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt
     4441 aagccgcgtg gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg
     4501 gcgtcatcaa gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc
     4561 gccttctctt tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg
     4621 gcggcgaccg gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt
     4681 tcgcggattt ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga
     4741 ggcactcggt tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg
     4801 gagcgttttg tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc
     4861 gattgtgcgc cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg
     4921 ccgctgcaac tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt
     4981 cgtctggcgg cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc
     5041 gataccgttg gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa
     5101 gtggttaccg aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt
     5161 ggattggtga tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg
     5221 ctgttactcg atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct
     5281 acgctggaca atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa
     5341 gggcgctcgt ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac
     5401 gaagccgttg cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta
     5461 tttttacctg gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc
     5521 agtgatgtgg tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg
     5581 atcctcccgg caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc
     5641 agtttaacca tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc
     5701 tttgatccgc gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg
     5761 acgcagcgtg ccgggcgagc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc
     5821 aaagaacaag cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt
     5881 tccggtttgc tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg
     5941 ctggatcaac cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg
     6001 gcgctggagg gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat
     6061 ccgcgtttag cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca
     6121 aaaattgccg ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg
     6181 ttttcgcgca atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac
     6241 gtacgcggcg gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc
     6301 gatcgcattg ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga
     6361 gcgatgctcg atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta
     6421 ttgcagggca gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat
     6481 gaattagtac aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac
     6541 gcgcaaggta cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg
     6601 cagccgctgg cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt
     6661 gataaaggtt taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg
     6721 ttatgcgccg caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg
     6781 ttggcgacgc tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc
     6841 ctgaaatcac tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa
     6901 cgtctggata gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt
     6961 cgttatcatg aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag
     7021 gccactaatc cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct
     7081 gcccaaaggc cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt
     7141 gaggtgcaaa aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca
     7201 aacacggcac cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc
     7261 tgtgggagag ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct
     7321 ccctcaaggg agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg
     7381 taacagaaga acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc
     7441 gggaatgacc gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag
     7501 gtaagccgtc gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat
     7561 gaagaaccga tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa
     7621 cgcggctggc tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt
     7681 tacggcgttt atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg
     7741 cccgcggcag tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac
     7801 gaaatggtga agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct
     7861 ggcgaattta ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg
     7921 gacagtaaag aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc
     7981 atcgtcaata tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc
     8041 accatgatct cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct
     8101 gatttgctgg tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga
     8161 atcagtctct actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg
     8221 cagggtgcga gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt
     8281 tcttactggc gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc
     8341 aaagaccgta ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac
     8401 gaaatccgcg gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta
     8461 agcctcgacc agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg
     8521 tggcgtaacc cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag
     8581 caacagatta ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag
     8641 ccgcgtggtg gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg
     8701 caggcaaaac tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt
     8761 gactcggtgg cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag
     8821 aaacagcgta agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt
     8881 gaagttcgtg cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg
     8941 caggcgcgtc gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc
     9001 cagccgaaaa tctatcgcct gaacacatgg attgccgatg cgccaatcgc gctgcgtcag
     9061 ccgaatggcc aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga
     9121 gtgatgctgg tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg
     9181 gcgctggggc tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag
     9241 ttgcatccgg ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg
     9301 caggcattcc agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg
     9361 gtaatcgcgg aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt
     9421 ccggcgcagg tggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg
     9481 ggtcgtcagc ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc
     9541 aacaataacg tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg
     9601 gtaggccgtg ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt
     9661 tatcagcgtt atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat
     9721 attgcagata tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc
     9781 ttgccggtct ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag
     9841 ccgtcaggca atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct
     9901 gctcagcaag agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt
     9961 agtaattaac atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat
    10021 cccatctaag atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc
    10081 tcagattgcg taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat
    10141 aatcattctc gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc
    10201 aaaactgctc agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt
    10261 agcggcatgt ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt
    10321 accgctgcac ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg
    10381 cagtcagcta ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct
    10441 gttgtgaccg ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc
    10501 tacacgccgg gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc
    10561 attcgcggtt ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg
    10621 cagggcaact tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt
    10681 atgcgtggcc cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg
    10741 gtcagcaagc gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac
    10801 agcctgttcc agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct
    10861 tatcgcctga ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag
    10921 cgttatgcta ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc
    10981 ctttcttact tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga
    11041 accgttgagc cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag
    11101 aacaacacct attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac
    11161 gacaccttta ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc
    11221 gtttatggtt acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca
    11281 ttagcgccag cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag
    11341 ctgcaaaact tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac
    11401 cacaccctgc tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt
    11461 ggttacgacg actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac
    11521 ttcaatgcca aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa
    11581 acgggcgttt atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt
    11641 cgttatgact gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt
    11701 gatgacaaac agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca
    11761 ccttacttca gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat
    11821 attttcgcac cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat
    11881 cgtccgattg tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg
    11941 gaccctgagg gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa
    12001 atcgaagcga aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc
    12061 gatgcggaat acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa
    12121 cacatggctt cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg
    12181 ctgggcaccg gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa
    12241 gtgggaagtt atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg
    12301 gcgggctcca acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc
    12361 tgctttaaca cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc
    12421 tttcgtttct aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg
    12481 ttatgcagga atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc
    12541 gtgtgcccgg gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga
    12601 ccggtctgat tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc
    12661 atcagcctcc gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca
    12721 gcaaagcgtt tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga
    12781 tgaccgtgcg tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct
    12841 ttggtgcggc agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc
    12901 tggcgcatcg gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca
    12961 tgctggtggc gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata
    13021 tcgcccacca ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga
    13081 cggtcattgc cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg
    13141 ccctgcgcag tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa
    13201 ccctcgaaat gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac
    13261 ctgtgagttt tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac
    13321 ggcgatggcg ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga
    13381 ccccaatcgt attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat
    13441 cgtgccttac ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt
    13501 gccggactca gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga
    13561 aatgaaaccg tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc
    13621 acgtattgcg ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc
    13681 gcgtaaatcg ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca
    13741 tttagcgcaa tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc
    13801 acgcccgtta ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa
    13861 cagcttgttc caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac
    13921 caacttctgg ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga
    13981 tgtgctctgt tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct
    14041 gtggcaggcc atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt
    14101 ttatggtgca acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg
    14161 taaagcgtga gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt
    14221 gtcgctacgg cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg
    14281 caggccgcct ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg
    14341 ttgccgcgtc tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg
    14401 tttcagcaag tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc
    14461 gcgcaactgg ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag
    14521 tttgctgcgc tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg
    14581 aaacggctgt cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc
    14641 gcaatcaatc agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg
    14701 agcactggaa cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg
    14761 ctgggcggtg tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat
    14821 gatggcgtgg cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg
    14881 ctggcgattg tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg
    14941 ttgttcgcgc cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg
    15001 ctggcgtcgt tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg
    15061 actcgcgtgt ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg
    15121 ctactgtggc tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat
    15181 cgtgtcgcga ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg
    15241 ttgatggctg tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg
    15301 agcggggcgt tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg
    15361 tttgcgggcg tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg
    15421 atggcaagcc cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg
    15481 ctgtttctgg taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg
    15541 gcggtgacgc tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc
    15601 atgttactgg cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag
    15661 gcaagtggtg acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac
    15721 gcgaccgatg ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact
    15781 ccgctgtgcc gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta
    15841 gggatggcgc tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg
    15901 accgcgacga tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg
    15961 cggatgatgg gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt
    16021 ttactgctgg tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg
    16081 gcggggctgc tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag
    16141 agccgttaat agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca
    16201 cccgacgtgc agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa
    16261 agcctgcttc aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga
    16321 aacgcttaaa acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg
    16381 cgtcggtaaa gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg
    16441 cttcttctgc cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt
    16501 cgtgaatgcc cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg
    16561 ggttaccgga aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta
    16621 catcacgacg accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc
    16681 aggttaaatc tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac
    16741 cggtcatcac ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac
    16801 ctggcaggaa ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga
    16861 taatacaggc aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt
    16921 tataagtaca ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct
    16981 ggcctaacgt gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt
    17041 aacaaccttc aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg
    17101 cgctcatcgt cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca
    17161 gttcggtcac cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac
    17221 cacgctcggc ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca
    17281 tcggccccca ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag
    17341 cgccgtccgc tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag
    17401 gggagttcac accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac
    17461 tcatggaggg ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa
    17521 caagttaaat actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga
    17581 gtataaaagt tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc
    17641 gagtaaaatg ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat
    17701 actccctctt tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa
    17761 cttcttgagc gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg
    17821 cttgtcgggc tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt
    17881 atgggggcgc tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc
    17941 tgttcggcgg tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca
    18001 gggggatcgg ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg
    18061 tggcgtgtat tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg
    18121 ctagggcgcg aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat
    18181 attttccgcc tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg
    18241 gggctggctg cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg
    18301 cgtccgcagt ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg
    18361 tcgaccatta tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt
    18421 tctgatgcgc cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt
    18481 ttcggcccta tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac
    18541 ggcggcaata ttaccaaatg ggtactgatg gggggggcga ttggcggtct gtgtggattg
    18601 ctggggtttg tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc
    18661 gcggggaatt tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc
    18721 ttactctgct tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt
    18781 actgtgctgg gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg
    18841 gaggcgggga cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg
    18901 ccgttaacgg ggatcattct ggttctggag atgaccgata actaccagct cattttgcca
    18961 atgattatta ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta
    19021 tactcggcga ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc
    19081 aaggccgcat cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga
    19141 ttattattgg gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat
    19201 gacgtagcac tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc
    19261 gctgacgaag ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc
    19321 ggcttccagt atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa
    19381 aaacagggcg ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc
    19441 gttgactaca ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa
    19501 agcacctgcg gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa
    19561 ctatccggca catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct
    19621 taagatgcca acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac
    19681 tggctgtttc cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga
    19741 taatacaggc agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta
    19801 catcacgaat gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg
    19861 gaccggcttc cgcattaaag gctttattca cgctaatgcc gacaaacacc gccagaccaa
    19921 cggcgtccag caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga
    19981 tggtcagcat gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata
    20041 ccgggccgtg atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc
    20101 ccagtaccag aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga
    20161 tggcaaatac cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac
    20221 tgagcacctg aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt
    20281 gctgcgagga taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg
    20341 ggaattttga gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct
    20401 gtaatgacaa tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag
    20461 ggaacccggc tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga
    20521 atcgactctt ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt
    20581 ttaggtttat cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg
    20641 gcttgttcgg ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca
    20701 atttgttcaa tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg
    20761 tcaacctgcc gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg
    20821 acaatgcgtt ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt
    20881 ggaggatagt cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt
    20941 tcagtgttgg cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg
    21001 agaaaagaca gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac
    21061 cagtgactca accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct
    21121 aagatgagac tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt
    21181 gaaattgtgg cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg
    21241 gaagttgtgg cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac
    21301 aatcaggcca cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat
    21361 cagtttatcg tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc
    21421 aaatgccgtg acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac
    21481 tttcagcgtt ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca
    21541 gtgttccaac agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag
    21601 aagcgcaacc tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat
    21661 agtttgacgg ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat
    21721 gatgccgatt ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg
    21781 cgcttacggg gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa
    21841 aaaaaataaa ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga
    21901 tcctgcggat cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc
    21961 tgcaacaaaa gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta
    22021 cccactcgat ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc
    22081 tgaaagagct taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa
    22141 gcattgttga gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg
    22201 gcgaagcagc gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc
    22261 agcctctgac tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac
    22321 cgcttaacga gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac
    22381 aaggcattcg tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg
    22441 gtattttaaa atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact
    22501 atttaatgaa aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta
    22561 aagaacttaa tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg
    22621 acgacatctc ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg
    22681 ttgagcagct ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct
    22741 tttcgctggt ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta
    22801 cggaagatca gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg
    22861 cggcacaacg atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat
    22921 tattggaaga tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa
    22981 aacatgtgtt tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta
    23041 gcggattatt agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac
    23101 tggtagaaaa agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct
    23161 cgacgcgcca tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg
    23221 agtttccgct atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta
    23281 tgaccgacct ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag
    23341 tcttttgtaa agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc
    23401 gggctataaa acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga
    23461 aatacatgaa aaaaaacaca ttagcactga gtgcactggc tctgagttta ggtttggcgt
    23521 tatctccgct ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc
    23581 caagccttgc accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag
    23641 gtagcacaac cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg
    23701 attctccgtt ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc
    23761 agggcggtaa tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca
    23821 ttgatgccga taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga
    23881 ttaaagtcca gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc
    23941 gctctgatat cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg
    24001 cggattctga tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc
    24061 tgggcgagac ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg
    24121 aaaactacga aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg
    24181 cgctggttaa cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg
    24241 gcggcaacat cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc
    24301 agatggtgga atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga
    24361 attccgaact ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg
    24421 ttctgcctaa ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac
    24481 tgaacggtaa gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg
    24541 tgggcagcaa actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg
    24601 aactgcagca gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag
    24661 gcgctgagat gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg
    24721 gcactccggc tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc
    24781 aggcagtgaa aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg
    24841 cactgaacat tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa
    24901 ccccttcctg aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca
    24961 tacatcttca tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc
    25021 taagccgtgc ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag
    25081 gatatcgtgg cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc
    25141 gggcgaatta tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg
    25201 ctggtacttt cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac
    25261 ggtgtgcggc aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt
    25321 ggcctgacag gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct
    25381 gaaatgatgc tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg
    25441 gaagaactgg tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg
    25501 cgcaacggct ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca
    25561 gcggtcagct tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga
    25621 ctacgcccga gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga
    25681 aaccggcgtt gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac
    25741 aactgattac ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact
    25801 attttaccgg tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg
    25861 tgggtaaaca gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg
    25921 tgttactcga cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc
    25981 ggctgaaagc gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc
    26041 acaatgtgca accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt
    26101 atcggcttaa tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt
    26161 tgctgctgta tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag
    26221 gcggcgctgg ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc
    26281 aattcgggat tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt
    26341 ggttacgact gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc
    26401 gctgcggtca cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct
    26461 gatttcctgg acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg
    26521 acgcggatat ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata
    26581 gattttcaga atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg
    26641 attggtaata cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt
    26701 aaattgtgct tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag
    26761 cctttttctt ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg
    26821 gcgatattcc atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc
    26881 cgaaacaaga tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt
    26941 ctgagccaga aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg
    27001 tacgcagcag ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga
    27061 taaccgcaca gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca
    27121 cagaccccgc cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg
    27181 tgctctgacc aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt
    27241 caaccacttc agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct
    27301 gcagcggctc cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa
    27361 tctgcgcaca agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg
    27421 cgccgatgtt aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac
    27481 gtaccgccgc tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt
    27541 cggcgaattt catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat
    27601 tatcattaat acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact
    27661 gaccgtcaat tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat
    27721 ttaccgcttc gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa
    27781 aagcggtttc aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa
    27841 cacatcaccc tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct
    27901 gcaactcgtt attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc
    27961 gctcgccaat ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt
    28021 tcccgactcg cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc
    28081 ggtcggtatg ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg
    28141 cgggttggcg acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac
    28201 caaaccgaat aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga
    28261 taaaggtatc catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta
    28321 aattgcggcg gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc
    28381 agataaaaat ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc
    28441 ttaaatcatg ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga
    28501 caaaatagtt agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat
    28561 ccatgcgcag tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg
    28621 gcgtgttttg cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca
    28681 gactttgctt ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca
    28741 ggcataccag atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga
    28801 cttcagggtc ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa
    28861 ccagccaggc aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca
    28921 ctacatcctg agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa
    28981 acagcgcggc gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca
    29041 acggatggcg ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga
    29101 tagtatgttc atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat
    29161 gcgaccattg cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc
    29221 gccgcaccgc tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat
    29281 tacacagcgg ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt
    29341 aaatgccggt gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg
    29401 gctggcgcat aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact
    29461 catcgtcgag tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca
    29521 gttgcagcag catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca
    29581 tcatccgctc gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa
    29641 gttggcgatc gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc
    29701 gaatacgcca cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga
    29761 agccaaaccc gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt
    29821 gcagagtgtg aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt
    29881 agctggtgcc atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga
    29941 acttgtcgga aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg
    30001 caacatcgcc aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata
    30061 acccttccaa catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat
    30121 cccagagcag cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac
    30181 ggcttaaaat cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac
    30241 cgacggcaac caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt
    30301 gcaggagctg gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat
    30361 tgtcaaaggc atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga
    30421 cggttaattc atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga
    30481 gagcggtgtt tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa
    30541 ttatcgcatt aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc
    30601 gccgggatgg tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca
    30661 atagtatgct catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct
    30721 ttcatggtgc ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc
    30781 cccggtttca gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca
    30841 tggaagccgc gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct
    30901 tcgacaaatt tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg
    30961 cgtagcgcca ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc
    31021 ggcttaccga cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg
    31081 acatcaatgt taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg
    31141 cacaccactt cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag
    31201 gcagaaaccg cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg
    31261 gtgctgacgc ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg
    31321 ccagcgacgc gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct
    31381 gtccatcagc gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc
    31441 cgggcaaatt gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat
    31501 agaaaattcg acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat
    31561 ttgtggtata aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc
    31621 gacaatctca ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg
    31681 gggtcggtaa tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc
    31741 atggcaactg tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc
    31801 cgttactgga acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc
    31861 atcaaccttg agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt
    31921 gtttctcgca aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg
    31981 aaagacgctg ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg
    32041 ctgactaact ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag
    32101 tctcaggacg gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag
    32161 ctggagaaac tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct
    32221 ctgtttgtaa tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt
    32281 attccggtat ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc
    32341 ccgggtaacg acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc
    32401 gtacgtgaag gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct
    32461 gagtaataag gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc
    32521 gagaattccc gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat
    32581 ctgcggcgcg aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta
    32641 gggggcctgc atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag
    32701 atcacatctc cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc
    32761 gtgagcgtac tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg
    32821 acatcgagct ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag
    32881 caggcaacgt tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca
    32941 ttctggaagt taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg
    33001 cagacaaagt tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag
    33061 cacagttcga agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc
    33121 gccgcgttgc tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg
    33181 gcgttctggt tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg
    33241 ttgctgcaag caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa
    33301 aagaatacca ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag
    33361 agaaaatggt tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc
    33421 cgttcgttat ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag
    33481 tgactggctt catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg
    33541 cagcagaagt tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg
    33601 cggcttcttt ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa
    33661 gcgacgtaca atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa
    33721 catggctacc aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc
    33781 tctgcagggc actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga
    33841 aatcaaagaa ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct
    33901 gttccgtggc gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg
    33961 gatgctggcg accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt
    34021 gaacgctcgt ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc
    34081 agaagctatc agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa
    34141 cccgttcttt accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt
    34201 ggtgctgaaa gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac
    34261 cgcaaccatg tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat
    34321 ggacctggcg gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat
    34381 gaacaaaccg ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac
    34441 ggaataattc ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt
    34501 aagtctggtt ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac
    34561 cggagcgtac aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa
    34621 aatagcctaa taatccagac gattacccgt aatatgttta atcagggcta tacttagcac
    34681 acttccactg tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt
    34741 gattagcgat atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa
    34801 aacccaaatc agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt
    34861 cgtggaatat tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga
    34921 ttcccgtact ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc
    34981 gattatggcc tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc
    35041 gctgccgccg ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc
    35101 agaacaagcg cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc
    35161 actgttgaaa gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca
    35221 gaaactgact gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga
    35281 actgatgcag ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc
    35341 ggctggcggc gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca
    35401 ccattctggg ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc
    35461 ccgaacactt ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac
    35521 agtgcctgga attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc
    35581 ttaaaacgat gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg
    35641 cttgcgatat ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg
    35701 ctgggctgtt acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata
    35761 aagaatcact ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc
    35821 aattgttacc ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc
    35881 agcataatct gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg
    35941 ggtctggtgg ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc
    36001 aagcttgccg tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca
    36061 tgatgaacaa aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc
    36121 agatggaagt gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg
    36181 gcagtgttct ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg
    36241 catggccgaa tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt
    36301 tgacatttgc cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt
    36361 tcgaacaagg ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg
    36421 cttttcttgc gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg
    36481 aaaaaatgga tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg
    36541 cgagtgaagt cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca
    36601 gagagtcgcg ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt
    36661 aagtagttgg ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag
    36721 gcttcagtat tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc
    36781 tgctactcaa ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat
    36841 tatggacggc aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa
    36901 agccggggca aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc
    36961 gttaacgctg tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc
    37021 gttaatggaa ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa
    37081 cgtgcgtctg cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat
    37141 tcgtaaatct gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa
    37201 ctacggtgga cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca
    37261 aggaaacctg caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca
    37321 tgaactggcc cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt
    37381 tttgctttgg caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt
    37441 cgatgaacaa gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg
    37501 cggcaccgag cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct
    37561 gatatctgct tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt
    37621 ggggttcgcc attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct
    37681 tagcggtttt accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt
    37741 ggcgctgatg ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga
    37801 aatctcactt tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta
    37861 cccaggttcc gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct
    37921 aaccattgtt cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa
    37981 tcattacagt ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc
    38041 cggcgcatat atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc
    38101 gggtaaaacc tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg
    38161 ttatggcatg tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt
    38221 cgcagcgtta gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg
    38281 aattaaggac agcggtcatt taattccagg acacggtggt attttagatc gtattgatag
    38341 cctgacggct gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta
    38401 acggaaggta atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt
    38461 gtacttatca ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc
    38521 gttgagcgtt tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc
    38581 accgaatatg ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc
    38641 gcagaaccgg tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa
    38701 cgagcggcga ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac
    38761 tggctggttt ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc
    38821 aattcgatag ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt
    38881 atcgaaacgc ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa
    38941 agcaccacca ttacagtagc gccatttgac agcgaccaac ggcgggatgt aaagctcgat
    39001 ttacgtcact gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt
    39061 cctcgtgggc cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc
    39121 aaggcaggtt tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag
    39181 tgggtgacct ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc
    39241 gaaaggcagg ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt
    39301 aaagcgattg gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa
    39361 gttgtacgcc agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag
    39421 ctgatgaagc tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac
    39481 aacctcagtg ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta
    39541 gtttattacc tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt
    39601 ccgttgcccg tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc
    39661 ggaccggtat ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg
    39721 ctgttaatgg ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag
    39781 aacgcataat aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg
    39841 ccaccgtata cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc
    39901 gtgtcgccgt tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg
    39961 atgaagatat cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc
    40021 gcgtccttcg tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca
    40081 gcattacttt ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg
    40141 cttctggtgt gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag
    40201 gtctggaaga cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga
    40261 ccccgctgcc gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg
    40321 aaatccagca aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc
    40381 atttccaact gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga
    40441 aacagaaact ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg
    40501 cccgtttcaa catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg
    40561 tcacggtgaa catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca
    40621 accttgccgg gcactccgct gaaattgagc aactgactaa gatcgagccg ggcgagctgt
    40681 ataacggcac taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg
    40741 gttatgccta tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta
    40801 aattacgcgt gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag
    40861 gtaacgatac ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat
    40921 ggttggggag cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg
    40981 aaactgtcga taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct
    41041 acaaggtgaa agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa
    41101 gtggcgtgag cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg
    41161 ttggtatcaa cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc
    41221 cgtacttcac cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg
    41281 cagatgacgc cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg
    41341 gcttcccgat taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc
    41401 tgtccaacat gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc
    41461 cgagcacctc tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt
    41521 ggacctataa caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga
    41581 ccggtaaagt gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg
    41641 cgacttatgt gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg
    41701 gttatggtga tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg
    41761 gttccagcac cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc
    41821 cgcatcaggc cagtaattat gatccggact atgattacga atgtgcgact caggacggcg
    41881 cgaaagacct gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc
    41941 tcgagttcat caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt
    42001 cattcttctg ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg
    42061 gatatccgga ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat
    42121 ggatgtcccc attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg
    42181 gagacaaggc agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa
    42241 aggaatgtag tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac
    42301 gcaaagaact gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg
    42361 ttattagctg caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt
    42421 gcaatcgtca atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac
    42481 acgctggaaa atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg
    42541 caggctaaaa tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa
    42601 aaagacgtga tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat
    42661 cgcgcacgtc gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg
    42721 aaatccgttg ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac
    42781 aacagcagcg atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg
    42841 ccttcaattc gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc
    42901 gatatcgtca tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc
    42961 atggttaacc caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg
    43021 acccaggacg atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg
    43081 acttacgcgc gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca
    43141 cccagtgcgg tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac
    43201 gcggtgattg agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc
    43261 gtaggtaaaa acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac
    43321 catgagatcc agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac
    43381 ggctttggtt atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta
    43441 attattggcg atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat
    43501 gacactgtta ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc
    43561 gtgattggcg acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt
    43621 ggtcgttact gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac
    43681 aaagtgacgg ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat
    43741 tcctcaggca ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg
    43801 aacattgatg acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac
    43861 taacgttcca tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca
    43921 ggccgtgtta ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca
    43981 ctcatactct gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac
    44041 tggtggatcg cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct
    44101 ctgtcaatga gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc
    44161 tgattctgga agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac
    44221 tggaaccggg tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg
    44281 tcgtgcctgg cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga
    44341 cccgttttaa aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt
    44401 gtgctcgtag ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg
    44461 ccattgtgga agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg
    44521 ttggacccca tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg
    44581 gtcatactaa aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta
    44641 accaggattt gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca
    44701 ttcgcgaaag cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg
    44761 gcagcgacaa cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc
    44821 gctgtattct cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga
    44881 tcatcggcgg catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg
    44941 gcggctgctc cggtgtggcg caggacgtcc ctccttatgt tattgcgcag ggtaaccacg
    45001 caacgccgtt cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga
    45061 ttaccgctat ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag
    45121 tgaaaccgga aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt
    45181 tctttgcacg ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat
    45241 tgccctggtc gccggagaaa cctccggcga tatcctaggg gccggtttaa tccgcgctct
    45301 gaaagaacgt gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga
    45361 aggctgcgaa gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct
    45421 cggtcgtctg cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact
    45481 gaagccagat gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa
    45541 cctcaaaaag cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg
    45601 acagaaacgc gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt
    45661 cgaaaaagcg ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc
    45721 tgatgccatg ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta
    45781 cgatgcccac tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag
    45841 tgccgatttc ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt
    45901 ggtgccgctg gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc
    45961 gccagacctg tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga
    46021 tgcggcacta ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat
    46081 ggtggtggga tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac
    46141 tgattatgtc tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca
    46201 ggaagagtgt gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa
    46261 aaccagccac gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc
    46321 cgatgagcag gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg
    46381 cacacgcagc tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc
    46441 gtcaccgctg cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa
    46501 aagctgagcg aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc
    46561 tggagtctgg gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc
    46621 atgctggcga tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt
    46681 gatggtaacc gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc
    46741 cgcgtaccag aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg
    46801 gcggcgctgg atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc
    46861 gcttttcatc tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt
    46921 gggcctgtca aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac
    46981 cggaatctga agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg
    47041 atgatcgatg gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg
    47101 ccagcactgg cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg
    47161 ggacatggcg cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg
    47221 ctgggtgatg agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat
    47281 ctggcgttgc tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc
    47341 gatcgcgact ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg
    47401 ggcgacgtcg gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg
    47461 ttttatgaag aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg
    47521 gatgaagaaa gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc
    47581 gtggcgacta acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc
    47641 gtcgcgatcc acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg
    47701 cagcaatata tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc
    47761 cttgccaaca ccgttgagat cgccaaacgc tgtaacgtaa ctgtgcgtct tggtgaatac
    47821 ttcctgccgc agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca
    47881 aaagaaggcc tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa
    47941 cgccgcccgg aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc
    48001 ttcccgggct acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta
    48061 ccggtagggc caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc
    48121 accgacctcg atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt
    48181 gtctccatgc ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa
    48241 cacgtggcgg acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg
    48301 gcggcgaaag cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc
    48361 gaccgtatct cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa
    48421 gccgagccgc agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac
    48481 atggcgcgca aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt
    48541 atcgcgccga ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat
    48601 ccggtcaccc agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc
    48661 cttggtttgc gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg
    48721 gcgaagaatg gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc
    48781 ttcgacatgc tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg
    48841 aaggacctga tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca
    48901 ctgttccgcc ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat
    48961 ggtcgtgaag agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta
    49021 ctggagccaa cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg
    49081 ctttctggtt ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag
    49141 ccggaagaga tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc
    49201 aacgccgaac tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt
    49261 aacaaatcgc actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg
    49321 cactatccgg cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag
    49381 gtggtgggcc tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata
    49441 aactccggtc tttaccattt ccacgtcaac gacgatggcg aaatcgtgta tggtattggc
    49501 gccatcaaag gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc
    49561 ggctacttcc gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg
    49621 cgggtgctgg aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg
    49681 gcgctgatga actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa
    49741 gctatcggtc aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa
    49801 tcctacgcca gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg
    49861 ttaggcctgt acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat
    49921 gtcggaggcg taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct
    49981 gcggggctcg ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc
    50041 tgcacgctgg atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat
    50101 aaataccagc aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt
    50161 gatgacttca gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc
    50221 cgggaaaaat atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag
    50281 cttttaaacc gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat
    50341 ctctactatc agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc
    50401 tctccgagcg atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa
    50461 ctggagtttg actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg
    50521 attgcagagc tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa
    50581 ctggatatta acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg
    50641 cgtaaaatct tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag
    50701 cgtccttata ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc
    50761 gaccgtgcgt atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt
    50821 ccggtgatga tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac
    50881 tttggtatgc cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa
    50941 cgctttaaga tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc
    51001 gcagaagagc gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt
    51061 ggcgtaccgg tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt
    51121 ggcgtgggcg ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa
    51181 ggttgtgcgt ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg
    51241 ggtatcatcg ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg
    51301 ctgggcggcg ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg
    51361 gcggatctgg ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag
    51421 cgcctgatga gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt
    51481 ggcccttttt tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg
    51541 aacacatgaa catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca
    51601 tcaaagaact ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca
    51661 gcgttgattt gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact
    51721 gggatgagta cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc
    51781 tttatgcctt catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg
    51841 cgctctggtt ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc
    51901 agtacaccaa cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct
    51961 acgtcaaaga gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc
    52021 aaaaaagccc ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg
    52081 atgtctctat ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacacctgg
    52141 aagcggaaga gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg
    52201 gaatatcgac gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt
    52261 tgatcgaccg caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc
    52321 cagtctggct gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg
    52381 aatttactcg cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg
    52441 ttcatgcggt gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca
    52501 aacagacgct ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt
    52561 ttcatccaat ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt
    52621 tcttcgaaac gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc
    52681 acattaaagg cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca
    52741 cctcgcccag ttatcccatt gttgcttcgg ttaagacggc ggcggcgatg ctgcgtggta
    52801 atccgggcaa acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg
    52861 tccagcggct gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg
    52921 tggatgaagc cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg
    52981 cggatgccga tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg
    53041 acgaacaggg caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg
    53101 acgaacgtgg gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta
    53161 ttggcatcga taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct
    53221 cttacgatct caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg
    53281 atttctaccg caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta
    53341 aacacgatct ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga
    53401 cgccacatca ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac
    53461 aactggtcgg tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt
    53521 tgatgccagg agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc
    53581 tttgttccgt agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg
    53641 acgaagacgg cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg
    53701 cttccgggcg agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca
    53761 ggttcaccat attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga
    53821 tattcttggt ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg
    53881 ggatttggcg cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga
    53941 gcgccccagc cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat
    54001 cgatgcggca gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga
    54061 tccatacacg caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact
    54121 gtatgagcag taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac
    54181 tgtaaaatcg caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga
    54241 ttctggtggc ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt
    54301 ggcggacgga aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg
    54361 ccaatgccga tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc
    54421 tggtggtcga acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc
    54481 aggcacgtta tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc
    54541 aacatctcga cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg
    54601 ccgggctttc ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt
    54661 tgcttgcccg cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga
    54721 ttgaagacga aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag
    54781 tgccgttatt gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac
    54841 tttgtgctga acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc
    54901 aaacgtcgca ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg
    54961 cggcgattat tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc
    55021 tggtgcgcat ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt
    55081 tgggcgcctt tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg
    55141 ggcaaagcga aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg
    55201 ggctgggaac cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag
    55261 cggtcagcgt gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac
    55321 gtaagctgaa gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc
    55381 cactgctgtt ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag
    55441 gtgtggctga aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga
    55501 aagccggata agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc
    55561 accaccgtac cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg
    55621 gtttcgccag cggcctcgac gaccaggtac tccacatttt tgcgggagac taaatcactg
    55681 gcttttgcct gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg
    55741 caggcgagct caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt
    55801 gacataatcg ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc
    55861 agaaagggcg tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc
    55921 cggaacgtat cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca
    55981 atatttttcc acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc
    56041 ctacgaagat aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag
    56101 cgccgcaact gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg
    56161 gcgcttaaag cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag
    56221 tttctcgatg cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg
    56281 ctgcgcttgc tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg
    56341 tgtgccgatg atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg
    56401 attgagctgg ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa
    56461 gtggcgctgt ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc
    56521 aaatgtgtgc agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa
    56581 gtaagcgcct ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc
    56641 tggttatcgg atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca
    56701 tgattgtgat ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc
    56761 gtgcgcaggg cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt
    56821 ttgacattcg ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc
    56881 atcatttgat cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg
    56941 aactgaaccg cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag
    57001 aacaaaaagc ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat
    57061 cgaaagcaca aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat
    57121 aaaaagaagg aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc
    57181 tttactctga tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct
    57241 gccgaactga aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa
    57301 ggaatcgaaa tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat
    57361 ctcggtgctc gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct
    57421 gacaagctgg tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat
    57481 gcgctggaga tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg
    57541 gaaccggcac aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat
    57601 atggctgatg cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat
    57661 aacgcagagc tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta
    57721 ctgtcagtag aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa
    57781 gtattagcgc ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg
    57841 cagtaaacct gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag
    57901 gcctacagca tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt
    57961 ttacgccgca tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa
    58021 agcgacccgc cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt
    58081 ctggcttaaa cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc
    58141 agcccgattg cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt
    58201 ccgaacaaaa ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca
    58261 ggtcgctgac gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc
    58321 gagtcaccat aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga
    58381 ttttattggc aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta
    58441 agacgaaaag cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag
    58501 cgcctgtcgc ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg
    58561 aagtgctgaa gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat
    58621 cgccgggttt gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt
    58681 gatgctggaa tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt
    58741 ctggctggct gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc
    58801 ggcagtacgc ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt
    58861 caggaccctt tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca
    58921 gtcttaatta actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg
    58981 aggttacggt cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac
    59041 atcacgcccg gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt
    59101 tcgctgtaca gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc
    59161 agaatcgcca cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag
    59221 ttctgctcaa tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc
    59281 aggatttggt tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg
    59341 gtacccagct ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg
    59401 ccatccgggc ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg
    59461 acatcgcgat cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg
    59521 aaatcactca tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga
    59581 cccagtgaac ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc
    59641 agcgggcttg caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg
    59701 cgcaccagca gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc
    59761 ttaaccgttt tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg
    59821 ttcggcgtat caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg
    59881 atagcttctg ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg
    59941 tcttcaccgc tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg
    60001 gtgtcggcct gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc
    60061 gcatacattg catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca
    60121 tctttcatca ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg
    60181 aacttggtct ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta
    60241 cgaatcaggt cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca
    60301 cggtcaacaa aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac
    60361 aaatcggctg gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct
    60421 tcacgcacga tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat
    60481 aacccggagg ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc
    60541 tcggcgtcgg caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg
    60601 ttacggttcc agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat
    60661 ttagtttacc agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca
    60721 gcgcaaagac ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc
    60781 agacggcata ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg
    60841 ccagtacttc gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct
    60901 gcttttcgac ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat
    60961 agctggcact ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta
    61021 ccggcgtacc atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc
    61081 aaacaacctc tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc
    61141 gtgttgcgaa aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac
    61201 gccagccgcc ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt
    61261 ccaggccgcg aacggcgtcg gcctggttgt aggaagctat gagatgcagt tcgccgttgg
    61321 cgctttttac cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa
    61381 taacgcctat ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc
    61441 gatacataca gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag
    61501 taatacagca ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg
    61561 aatgctcggc ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag
    61621 atcgcggaac ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc
    61681 gcgcggcgct ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg
    61741 ttcgacaggg gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat
    61801 cggtaaagca cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga
    61861 gcttgtaaaa atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa
    61921 acagcctaaa aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc
    61981 gtaaacttca tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt
    62041 atcttcacgc gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc
    62101 gtctttcgcc ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc
    62161 gatttgcgcg tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag
    62221 attttttggg ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag
    62281 tttgatcaag cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt
    62341 tggcacggca acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta
    62401 accagcaatc ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt
    62461 cagttgctga tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct
    62521 caatgcttcg tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata
    62581 tttgtctttc gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac
    62641 gccgacttta atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga
    62701 tccaatcagg gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt
    62761 cttcaattat ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc
    62821 gcctgcaaac tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac
    62881 cgtcgcgttg tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc
    62941 gactgcacca cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt
    63001 caccagaccc ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc
    63061 acccattgcg cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac
    63121 catacgggca ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc
    63181 aatcgatgta ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa
    63241 cgggatggaa cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc
    63301 aataatttgc cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa
    63361 cccaaaaaat ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag
    63421 ccacatcatc ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc
    63481 aggcaatggc ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga
    63541 atttaacgcc accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac
    63601 gacgcgcggt ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca
    63661 gcatcggcac gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga
    63721 tatccagatg cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga
    63781 acacttcact taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat
    63841 cacaaatacg cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac
    63901 ggcggttaat gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc
    63961 tggtggcttc atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca
    64021 cacgttgttt ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac
    64081 caaccaatga cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca
    64141 gctccagcgg cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct
    64201 ggaaaatcat accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca
    64261 attcctggcc atcgaccagc acgctaccct cggttgggcg ctccagcaag tttacacaac
    64321 gtataagcgt actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg
    64381 gcacatgcag gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt
    64441 tggtgatatt cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc
    64501 atctcgaatg cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg
    64561 tctaaatcaa tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta
    64621 gcgtcccatg ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc
    64681 ttgaccgtga tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg
    64741 aatttatcga cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg
    64801 tggtagtaac caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa
    64861 cgctgaccga gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct
    64921 attattgccc gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc
    64981 gcaaaccaca tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg
    65041 cttcttatat ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg
    65101 gaacaaaagt gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag
    65161 attgggtgtt aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg
    65221 cgcaatgatt aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga
    65281 gccgactccc tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca
    65341 gccggttcgg ttgaagagaa aaaatcctga aattcagggt tgactctgaa ataggaaagt
    65401 gtgatatacg ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa
    65461 tttatcagac aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacgata
    65521 aatgaatacc aagtctcaag agtgaacacg taattcatta cgaagtttaa ttctttgagc
    65581 atcaaacttt taaattgaag agtttgatca tggctcagat tgaacgctgg cggcaggcct
    65641 aacacatgca agtcgaacgg taacaggaag cgcttgctgc ttcgctgacg agtggcggac
    65701 gggtgagtaa tgtctgggaa actgcctgat ggagggggat aactactgga aacggtagct
    65761 aataccgcat aacgtcgcaa gaccaaagag ggggaccttc gggcctcttg ccatcggatg
    65821 tgcccagatg ggattagctt gttggtgggg taacggctca cc
//
LOCUS       DAZXUL010000005        60419 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZXUL010000005 DAZXUL010000000
VERSION     DAZXUL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.005"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..322
                     /locus_tag="ACH3L1_000280"
     CDS             <1..322
                     /locus_tag="ACH3L1_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4075202.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="ACH3L1_000281"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="ACH3L1_000281"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFT4075203.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="ACH3L1_000282"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="ACH3L1_000282"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFT4075204.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACH3L1_000283"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACH3L1_000283"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFT4075205.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="ACH3L1_000284"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="ACH3L1_000284"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFT4075206.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="ACH3L1_000285"
     CDS             complement(3726..4955)
                     /locus_tag="ACH3L1_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFT4075207.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRLPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="ACH3L1_000286"
     CDS             complement(5019..5459)
                     /locus_tag="ACH3L1_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFT4075208.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="ACH3L1_000287"
     CDS             complement(5564..6334)
                     /locus_tag="ACH3L1_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4075209.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="ACH3L1_000288"
     CDS             complement(6331..7257)
                     /locus_tag="ACH3L1_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFT4075210.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="ACH3L1_000289"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="ACH3L1_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFT4075211.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACH3L1_000290"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACH3L1_000290"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFT4075212.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="ACH3L1_000291"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="ACH3L1_000291"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFT4075213.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACH3L1_000292"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACH3L1_000292"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFT4075214.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMEHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="ACH3L1_000293"
     CDS             12964..13311
                     /locus_tag="ACH3L1_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFT4075215.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="ACH3L1_000294"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="ACH3L1_000294"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFT4075216.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="ACH3L1_000295"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="ACH3L1_000295"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFT4075217.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACH3L1_000296"
     CDS             complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACH3L1_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFT4075218.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACH3L1_000297"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACH3L1_000297"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFT4075219.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="ACH3L1_000298"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="ACH3L1_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACH3L1_000299"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACH3L1_000299"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFT4075220.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACH3L1_000300"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACH3L1_000300"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFT4075221.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACH3L1_000301"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACH3L1_000301"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFT4075222.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACH3L1_000302"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACH3L1_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFT4075223.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="ACH3L1_000303"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="ACH3L1_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFT4075224.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACH3L1_000304"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACH3L1_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFT4075225.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACH3L1_000305"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACH3L1_000305"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFT4075226.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="ACH3L1_000306"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="ACH3L1_000306"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFT4075227.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="ACH3L1_000307"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="ACH3L1_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFT4075228.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /locus_tag="ACH3L1_000308"
                     /pseudo
     CDS             32769..33916
                     /locus_tag="ACH3L1_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="ACH3L1_000309"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="ACH3L1_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFT4075229.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACH3L1_000310"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACH3L1_000310"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFT4075230.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="ACH3L1_000311"
                     /pseudo
     CDS             complement(36348..36386)
                     /locus_tag="ACH3L1_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="ACH3L1_000312"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="ACH3L1_000312"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFT4075231.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="ACH3L1_000313"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="ACH3L1_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFT4075232.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="ACH3L1_000314"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="ACH3L1_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFT4075233.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACH3L1_000315"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACH3L1_000315"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFT4075234.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACH3L1_000316"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACH3L1_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFT4075235.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACH3L1_000317"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACH3L1_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFT4075236.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACH3L1_000318"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACH3L1_000318"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFT4075237.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACH3L1_000319"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACH3L1_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFT4075238.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACH3L1_000320"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACH3L1_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFT4075239.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACH3L1_000321"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACH3L1_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFT4075240.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACH3L1_000322"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACH3L1_000322"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFT4075241.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACH3L1_000323"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACH3L1_000323"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFT4075242.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACH3L1_000324"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACH3L1_000324"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFT4075243.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACH3L1_000325"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACH3L1_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFT4075244.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACH3L1_000326"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACH3L1_000326"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFT4075245.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACH3L1_000327"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACH3L1_000327"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFT4075246.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACH3L1_000328"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACH3L1_000328"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFT4075247.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACH3L1_000329"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACH3L1_000329"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFT4075248.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACH3L1_000330"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACH3L1_000330"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFT4075249.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACH3L1_000331"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACH3L1_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFT4075250.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACH3L1_000332"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACH3L1_000332"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFT4075251.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACH3L1_000333"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACH3L1_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFT4075252.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14472 a        16731 c        14849 g        14367 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtagg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgct ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgtctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcaac aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gccccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattctag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAZXUL010000006        55800 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZXUL010000006 DAZXUL010000000
VERSION     DAZXUL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55800
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.006"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..846
                     /gene="ldtC"
                     /locus_tag="ACH3L1_000334"
     CDS             <1..846
                     /gene="ldtC"
                     /locus_tag="ACH3L1_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFT4075253.1"
                     /translation="LVGENKFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPR
                     AGSVLTIPLQTLLPDAPREGIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPT
                     MVTTVSDKRANPTWTPTANIRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYL
                     LHGTNADFGIGMRVSSGCIRLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLV
                     EVHQPLSEKIDDDPQLLPITLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQV
                     SPQTL"
     gene            complement(929..1141)
                     /locus_tag="ACH3L1_000335"
     CDS             complement(929..1141)
                     /locus_tag="ACH3L1_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFT4075254.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            1426..2058
                     /gene="comR"
                     /locus_tag="ACH3L1_000336"
     CDS             1426..2058
                     /gene="comR"
                     /locus_tag="ACH3L1_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFT4075255.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(2120..2659)
                     /locus_tag="ACH3L1_000337"
     CDS             complement(2120..2659)
                     /locus_tag="ACH3L1_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFT4075256.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(2886..4190)
                     /gene="ndh"
                     /locus_tag="ACH3L1_000338"
     CDS             complement(2886..4190)
                     /gene="ndh"
                     /locus_tag="ACH3L1_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFT4075257.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(4598..5129)
                     /gene="ycfP"
                     /locus_tag="ACH3L1_000339"
                     /pseudo
     CDS             complement(4598..5129)
                     /gene="ycfP"
                     /locus_tag="ACH3L1_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
     gene            complement(5152..6177)
                     /gene="nagZ"
                     /locus_tag="ACH3L1_000340"
     CDS             complement(5152..6177)
                     /gene="nagZ"
                     /locus_tag="ACH3L1_000340"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFT4075258.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(6188..7012)
                     /gene="thiK"
                     /locus_tag="ACH3L1_000341"
     CDS             complement(6188..7012)
                     /gene="thiK"
                     /locus_tag="ACH3L1_000341"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFT4075259.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(6993..7634)
                     /gene="lpoB"
                     /locus_tag="ACH3L1_000342"
     CDS             complement(6993..7634)
                     /gene="lpoB"
                     /locus_tag="ACH3L1_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFT4075260.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(7649..8026)
                     /locus_tag="ACH3L1_000343"
     CDS             complement(7649..8026)
                     /locus_tag="ACH3L1_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFT4075261.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(8029..8388)
                     /gene="hinT"
                     /locus_tag="ACH3L1_000344"
     CDS             complement(8029..8388)
                     /gene="hinT"
                     /locus_tag="ACH3L1_000344"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFT4075262.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            8721..10906
                     /gene="fhuE"
                     /locus_tag="ACH3L1_000345"
                     /pseudo
     CDS             8721..10906
                     /gene="fhuE"
                     /locus_tag="ACH3L1_000345"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(10966..12399)
                     /gene="ptsG"
                     /locus_tag="ACH3L1_000346"
     CDS             complement(10966..12399)
                     /gene="ptsG"
                     /locus_tag="ACH3L1_000346"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFT4075263.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(12694..13491)
                     /locus_tag="ACH3L1_000347"
     CDS             complement(12694..13491)
                     /locus_tag="ACH3L1_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFT4075264.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(13502..14506)
                     /gene="holB"
                     /locus_tag="ACH3L1_000348"
     CDS             complement(13502..14506)
                     /gene="holB"
                     /locus_tag="ACH3L1_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFT4075265.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(14503..15144)
                     /gene="tmk"
                     /locus_tag="ACH3L1_000349"
     CDS             complement(14503..15144)
                     /gene="tmk"
                     /locus_tag="ACH3L1_000349"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFT4075266.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(15134..16156)
                     /gene="yceG"
                     /locus_tag="ACH3L1_000350"
     CDS             complement(15134..16156)
                     /gene="yceG"
                     /locus_tag="ACH3L1_000350"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFT4075267.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(16159..16968)
                     /gene="pabC"
                     /locus_tag="ACH3L1_000351"
     CDS             complement(16159..16968)
                     /gene="pabC"
                     /locus_tag="ACH3L1_000351"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFT4075268.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(17088..18329)
                     /gene="fabF"
                     /locus_tag="ACH3L1_000352"
     CDS             complement(17088..18329)
                     /gene="fabF"
                     /locus_tag="ACH3L1_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFT4075269.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(18416..18652)
                     /gene="acpP"
                     /locus_tag="ACH3L1_000353"
     CDS             complement(18416..18652)
                     /gene="acpP"
                     /locus_tag="ACH3L1_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFT4075270.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(18863..19597)
                     /gene="fabG"
                     /locus_tag="ACH3L1_000354"
     CDS             complement(18863..19597)
                     /gene="fabG"
                     /locus_tag="ACH3L1_000354"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFT4075271.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(19610..20539)
                     /gene="fabD"
                     /locus_tag="ACH3L1_000355"
     CDS             complement(19610..20539)
                     /gene="fabD"
                     /locus_tag="ACH3L1_000355"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFT4075272.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(20555..21508)
                     /gene="fabH"
                     /locus_tag="ACH3L1_000356"
     CDS             complement(20555..21508)
                     /gene="fabH"
                     /locus_tag="ACH3L1_000356"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFT4075273.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(21576..22646)
                     /gene="plsX"
                     /locus_tag="ACH3L1_000357"
     CDS             complement(21576..22646)
                     /gene="plsX"
                     /locus_tag="ACH3L1_000357"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFT4075274.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(22727..22900)
                     /gene="rpmF"
                     /locus_tag="ACH3L1_000358"
     CDS             complement(22727..22900)
                     /gene="rpmF"
                     /locus_tag="ACH3L1_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFT4075275.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(22952..23473)
                     /gene="yceD"
                     /locus_tag="ACH3L1_000359"
     CDS             complement(22952..23473)
                     /gene="yceD"
                     /locus_tag="ACH3L1_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFT4075276.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            23672..24256
                     /gene="yceF"
                     /locus_tag="ACH3L1_000360"
     CDS             23672..24256
                     /gene="yceF"
                     /locus_tag="ACH3L1_000360"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFT4075277.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(24368..25327)
                     /gene="rluC"
                     /locus_tag="ACH3L1_000361"
     CDS             complement(24368..25327)
                     /gene="rluC"
                     /locus_tag="ACH3L1_000361"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFT4075278.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            25900..29091
                     /gene="rne"
                     /locus_tag="ACH3L1_000362"
     CDS             25900..29091
                     /gene="rne"
                     /locus_tag="ACH3L1_000362"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFT4075279.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(29288..30230)
                     /gene="flgL"
                     /locus_tag="ACH3L1_000363"
                     /pseudo
     CDS             complement(29288..30230)
                     /gene="flgL"
                     /locus_tag="ACH3L1_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(30242..31884)
                     /gene="flgK"
                     /locus_tag="ACH3L1_000364"
                     /pseudo
     CDS             complement(30242..31884)
                     /gene="flgK"
                     /locus_tag="ACH3L1_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(31950..32891)
                     /gene="flgJ"
                     /locus_tag="ACH3L1_000365"
     CDS             complement(31950..32891)
                     /gene="flgJ"
                     /locus_tag="ACH3L1_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFT4075280.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(32891..33988)
                     /gene="flgI"
                     /locus_tag="ACH3L1_000366"
     CDS             complement(32891..33988)
                     /gene="flgI"
                     /locus_tag="ACH3L1_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFT4075281.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(34000..34698)
                     /gene="flgH"
                     /locus_tag="ACH3L1_000367"
     CDS             complement(34000..34698)
                     /gene="flgH"
                     /locus_tag="ACH3L1_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFT4075282.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(34751..35533)
                     /gene="flgG"
                     /locus_tag="ACH3L1_000368"
     CDS             complement(34751..35533)
                     /gene="flgG"
                     /locus_tag="ACH3L1_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFT4075283.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSVLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(35671..36258)
                     /gene="flgF"
                     /locus_tag="ACH3L1_000369"
                     /pseudo
     CDS             complement(35671..36258)
                     /gene="flgF"
                     /locus_tag="ACH3L1_000369"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(36360..36422)
                     /locus_tag="ACH3L1_000370"
                     /pseudo
     CDS             complement(36360..36422)
                     /locus_tag="ACH3L1_000370"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36442..37581)
                     /locus_tag="ACH3L1_000371"
     CDS             complement(36442..37581)
                     /locus_tag="ACH3L1_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFT4075284.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(37606..38301)
                     /gene="flgD"
                     /locus_tag="ACH3L1_000372"
     CDS             complement(37606..38301)
                     /gene="flgD"
                     /locus_tag="ACH3L1_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFT4075285.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTILAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(38313..38716)
                     /gene="flgC"
                     /locus_tag="ACH3L1_000373"
                     /pseudo
     CDS             complement(38313..38716)
                     /gene="flgC"
                     /locus_tag="ACH3L1_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(38720..39136)
                     /gene="flgB"
                     /locus_tag="ACH3L1_000374"
     CDS             complement(38720..39136)
                     /gene="flgB"
                     /locus_tag="ACH3L1_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFT4075286.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            39291..39950
                     /gene="flgA"
                     /locus_tag="ACH3L1_000375"
     CDS             39291..39950
                     /gene="flgA"
                     /locus_tag="ACH3L1_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFT4075287.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            40026..40319
                     /gene="flgM"
                     /locus_tag="ACH3L1_000376"
     CDS             40026..40319
                     /gene="flgM"
                     /locus_tag="ACH3L1_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFT4075288.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            40324..40740
                     /gene="flgN"
                     /locus_tag="ACH3L1_000377"
     CDS             40324..40740
                     /gene="flgN"
                     /locus_tag="ACH3L1_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFT4075289.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(40780..42315)
                     /gene="murJ"
                     /locus_tag="ACH3L1_000378"
     CDS             complement(40780..42315)
                     /gene="murJ"
                     /locus_tag="ACH3L1_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFT4075290.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(42482..43348)
                     /locus_tag="ACH3L1_000379"
                     /pseudo
     CDS             complement(42482..43348)
                     /locus_tag="ACH3L1_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(43350..43997)
                     /locus_tag="ACH3L1_000380"
     CDS             complement(43350..43997)
                     /locus_tag="ACH3L1_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HFT4075291.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(44008..44592)
                     /gene="rimJ"
                     /locus_tag="ACH3L1_000381"
     CDS             complement(44008..44592)
                     /gene="rimJ"
                     /locus_tag="ACH3L1_000381"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFT4075292.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            44828..46036
                     /gene="mdtH"
                     /locus_tag="ACH3L1_000382"
     CDS             44828..46036
                     /gene="mdtH"
                     /locus_tag="ACH3L1_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFT4075293.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            46100..46747
                     /gene="grxB"
                     /locus_tag="ACH3L1_000383"
     CDS             46100..46747
                     /gene="grxB"
                     /locus_tag="ACH3L1_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFT4075294.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            46881..47441
                     /locus_tag="ACH3L1_000384"
     CDS             46881..47441
                     /locus_tag="ACH3L1_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFT4075295.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            47547..48593
                     /gene="pyrC"
                     /locus_tag="ACH3L1_000385"
     CDS             47547..48593
                     /gene="pyrC"
                     /locus_tag="ACH3L1_000385"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFT4075296.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            48667..48912
                     /gene="dinI"
                     /locus_tag="ACH3L1_000386"
     CDS             48667..48912
                     /gene="dinI"
                     /locus_tag="ACH3L1_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFT4075297.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            49202..49456
                     /gene="bssS"
                     /locus_tag="ACH3L1_000387"
     CDS             49202..49456
                     /gene="bssS"
                     /locus_tag="ACH3L1_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFT4075298.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            49571..50689
                     /gene="solA"
                     /locus_tag="ACH3L1_000388"
     CDS             49571..50689
                     /gene="solA"
                     /locus_tag="ACH3L1_000388"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFT4075299.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            50737..50850
                     /locus_tag="ACH3L1_000389"
     CDS             50737..50850
                     /locus_tag="ACH3L1_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFT4075300.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            51111..51677
                     /locus_tag="ACH3L1_000390"
     CDS             51111..51677
                     /locus_tag="ACH3L1_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFT4075301.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            51681..52256
                     /locus_tag="ACH3L1_000391"
     CDS             51681..52256
                     /locus_tag="ACH3L1_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFT4075302.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(52298..53350)
                     /locus_tag="ACH3L1_000392"
     CDS             complement(52298..53350)
                     /locus_tag="ACH3L1_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFT4075303.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            53575..54495
                     /gene="lpxL"
                     /locus_tag="ACH3L1_000393"
     CDS             53575..54495
                     /gene="lpxL"
                     /locus_tag="ACH3L1_000393"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFT4075304.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            54667..54852
                     /locus_tag="ACH3L1_000394"
                     /pseudo
     CDS             54667..54852
                     /locus_tag="ACH3L1_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            54888..>55800
                     /locus_tag="ACH3L1_000395"
     CDS             54888..>55800
                     /locus_tag="ACH3L1_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFT4075305.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIK"
BASE COUNT        13670 a        14809 c        13756 g        13565 t
ORIGIN      
        1 ctggttggcg aaaacaaatt tcatgtggtg gaaaatgacg gtggttctct ggaagccatc
       61 gccaaaaaat ataacgtcgg ctttctcgct ctgttacaag ctaaccccgg cgttgatcct
      121 tacgtaccgc gcgcgggcag cgtgttaacg atcccgttgc aaaccctact tccagacgcg
      181 ccgcgcgaag gcattgtgat caacattgcg gagctgcgtc tctattacta cccgccgggt
      241 aaaaattcgg taaccgtgta tccaataggt attggtcagt taggtggtga cacgctgaca
      301 ccgacaatgg tgaccaccgt ttcagacaaa cgtgcaaacc caacctggac gccaacggca
      361 aacatccgcg cccgttataa agcacaggga attgagttgc ctgcggtagt gccggctgga
      421 ccggataacc caatgggcca tcatgcgatt cgtctggcgg cctatggcgg cgtttatttg
      481 cttcacggta cgaacgccga tttcggcatt ggcatgcggg taagttctgg ctgtattcgt
      541 ctgcgggatg acgatatcaa aacactcttt agccaggtca ccccaggcac caaagtgaat
      601 atcatcaaca ctccgataaa agtctctgcc gaaccaaacg gtgcgcgtct ggttgaagta
      661 catcagccgc tgtcagagaa gattgatgac gatccgcagc tgctgccaat tacgctgaat
      721 agcgcaatgc aatcatttaa agatgcagca caaactgacg ctgaagtgat gcaacatgtg
      781 atggatgtcc gttccgggat gccggtggat gtccgccgtc atcaagtgag cccacaaact
      841 ctgtaaatcc tggataaaaa aaatccccgc gacacggcat gtcgcgggga aaaaatcagc
      901 agtagcaggc attaatgagg gttaatgctt atttataaat tactgctgtt ccatggaggg
      961 tattcggacc ggttacagaa gtaatacgga aagattttgc gcccatctca tccgcttttt
     1021 gcgccagctg ctcttccagc gatcccagat ttgtccccgc gttagcactg atagtaccga
     1081 ctttttgttg gccttctggc gttgactgaa cttcgacagc cgcaaagttg gcaaatgaca
     1141 tggagcttaa aatcgccgca gcgatgaggt ttttacgttt ttcataatag tggccttatg
     1201 cagatgaatg acgtcgcaag aaattactta acgatcgata actaaatgat agatgtgatc
     1261 tggatcacat acaagatatt ttttataaca atcattaatt aatttaaaaa tctttaaatt
     1321 tcagtcaaat agaaagcaat tatttttatg ggatctggac tggtgaacgg aggtgattat
     1381 ttttataata acctttcatt cacagtcacc aggtacaacg gcatcatggc aactgactca
     1441 acacaatgtg taaaaaaaag ccgtggccgc ccaaaagtgt tcgacaggga tgccgcgctt
     1501 gataaggcca tgaaattgtt ctggcaacac ggttatgaag cgacttctct tgcggacctc
     1561 gtcgaagcaa ccggagccaa agcgcccacg ctatacgcgg aatttaccaa caaagagggg
     1621 ttatttcgtg ccgttctcga ccgctatatc gatcgttttg ccgctaagca tgaagcacag
     1681 ctgttttgtg aagagaaaag cgtggagtct gcgctggctg actattttgc tgccatcgcc
     1741 aactgcttta ccagcaaaga caccccggca ggctgcttca tgatcaacaa ctgcaccacc
     1801 ctctccccag attcaggaga tatagccaat acgttgaaat cacgccatgc gatgcaagag
     1861 cgcactttgc agcagttttt atgtcaacga caagcgcgcg gggaaatccc gacccactgt
     1921 gacgtgacac atctggcaga attccttaat tgtattattc aggggatgtc gatcagcgca
     1981 cgcgaaggtg catcgctgga aaaactgatg cagattgccg gaacgacttt gcgtttatgg
     2041 cccgaactgg tgaaataagt cgcaaaaaag gcccctcagc ctgagcgaat gaggggccaa
     2101 attgcagagt acagccttgt tatactttgt tattcaaaat cagttgcccg ttactatcta
     2161 gcgggatctg cgtacccgga tcgcggtcca tgcggatttt gccctgctga tcgccaatct
     2221 tataggtcac atcataaccg agcatttttt ctgacttgtc atacaccgtt ttacaacgct
     2281 gttgcgtagt cgtgtaagta tcgctttcct ggagagagcc ctggatctgg ttaccggcat
     2341 atccaccacc cagcgccccc acaacagtgg cgacatcttt accgcgacca ccaccaaact
     2401 gatgccctat cacgccacca gcaacagcgc cgagcaccga cccggtaatg cgattttcat
     2461 cctgcaccgg tcgacgatgg gtcactgtga cgttgcgaca ctcctgacgc ggtgttttaa
     2521 ccgtttcctt gattggggtt gcagaaacaa cctgagcgta ttgcgggccc cgttcaaaca
     2581 cgttcagact ggccactgcc gctacgccca acgcagctgc gacaccaatc ccgatacccg
     2641 ccaacattga tttattcacg ggaacacctc cttctttgca gttatgccaa tcatgcaagc
     2701 gaaggagaaa agcagctatc agacagaaga tgaaaagcgc gggaatgact ggaaatactt
     2761 aaggattcgg aggagtctga aggaatgcct gatgcgacgc ttaacgcgtc ttatcaggcc
     2821 taccgctcgt accatttgtc cagggcggat aaggcgttta taccacatcc gccagtgtac
     2881 gtcgattaat gcaacttcaa acgcggacgg ataacgcggt taatactccc caccagcatc
     2941 attaatccgg ttttaaagta accatgcagc gcaatctgat gcattcggta tagcgagata
     3001 tatacaaagc gcgcaattcg tccttcaatc atcattgagc cgcgcgtcag gttacccatc
     3061 aggctaccaa cggtggagaa gttcgacagc gataccagcg aaccatgatc tttatactga
     3121 taatttttca gcggcttacc gttcatctgc gccagaatgt tgttcattgc gcaagtcgcc
     3181 atctggtgtg cagcctgagc acgcggcgga acaaagcccc cttccggacg cgggcatgac
     3241 gcgcagtcgc caatagcgta aatgtctgga tcgcgagtgg tttgcagcgt cggttccacc
     3301 accagctggt tgatacggtt agtttcaaga ccaccgatat ctttcaggaa gtctggcgct
     3361 ttgatcccgg ctgcccatac catcagatca gcctcaatat attcgccatc tttagtgtgc
     3421 aggccgcctt catcagcact ggtgaccatg gtttgcgtca gcacgcgaac gccaagtttc
     3481 gttagctcgt tgtgggccgc agcagagata cgtggcggta atgcaggcaa aatacgttct
     3541 cccgcttcta ccagcgttac gttcagggct tcgttggtca ggcctttgta accgtagctg
     3601 tgcagttgct tgatcgcgtt gtgcaattca gcggagagtt ctacacccgt cgcgccgccg
     3661 ccgacaatcg caatgttcac tttgccattt gcgcccaggt tggcagagta tttcaggaac
     3721 agattcagca tctcctggtg gaagcgacgc gcctggtgcg ggttatcgag gaaaatgcag
     3781 ttctctttga cgcctggcgt attgaaatcg ttagaggtgc tacccagcgc catgaccagg
     3841 gtgtcatagg cgattttacg ttccggaacc aacagttcac ctttctcgtc gcgtagttct
     3901 gcaatagtga ttgttttcgc ttcacgatca atatcaatga cggaacccaa ctggaactgg
     3961 aaaccatgat tgcgcgcatg ggccagatag ctcaacgcat cgacgccttc atcaagcgag
     4021 ccagtcgcca cttcgtgcag cagtggtttc cacaggtggc tgtggttacg atcgaccagc
     4081 gtaattttgg cttttttctt acgtcccagc ttatgcccca gctgtgttgc catttccagc
     4141 ccaccagcac cgccgccgac aatcacaatc tttttcaatg gcgtagtcaa cgtgcccccc
     4201 ttaaatttat taaccaattg ttaattaatg gttattaaca tagctcttaa ttaacaacag
     4261 gttacagcaa tgaaaatgtt cttcgctatt gagaataaca tgaatggtgc attggtcata
     4321 ccaaaattga tgttaatcaa gttttgttgc ttaaaagata ggcaacttta ccgggggaga
     4381 tcaaaaaata gagtttattg tttattattt gagcaaattc ggaagggtaa tatagggtga
     4441 agcgtatatc gagggcgttt ggtcgtcctg ataagatgcg cagcatcgca tcaggcattg
     4501 atgctcgact gtcggatgcg gcgtggacgc cttatccgac ttacagaatt cggagcgcat
     4561 caggtaccgg caaggttacc tgatgcgacg agggcattta cccgagggtt ttgaacgctt
     4621 taatgcgctg taaatgcggg gagatattct tgaatttgtg cgtctgctct tcgtcccaga
     4681 caatctcgta ataatgatgc aactcttcag atgtccgctg gctgttaagt gcttcatcat
     4741 tacgcgacaa aataaccaga caacgatcgc gattcttctc gcggaagttg gtcacacact
     4801 tagtggcaat atcggcatac tcttccgggc gatcaatctt gccttccatg ttctcataag
     4861 ggaacaaatt agggttgaag atcacctggc ggatatcgca aagaaaacca atccgttccg
     4921 cccaggccaa cgccgcaaat tagcggacgc tcgtcaacgt tcagttgcaa cattttgtcc
     4981 acttctttaa gcagatgctg catatcatgt ttcggatgcc gcgtactgta gcttatcaag
     5041 cgtacatccg ggtcaataaa ctgcaattgt aagactttct cgtggttacc cggactgtta
     5101 gagtcaaaac cgtgtaaata gataatcatc gcttcctcac ataagccagg gttagtgacc
     5161 tactttctct tcctgccagc gttcatgtaa ctgattcaga cgggtgctga tcgctttcca
     5221 gcgagccgag tccatcagtt cctgtcgcga aaatgaacct ttatgataca aacgtgtaac
     5281 acgttctgcc ttgatcggtg acagattatc taacacgctg acggcccctt tacgattatt
     5341 gcagaccagg atcatatcgc aacccgcatc cagtgatgcc tgcccgcgtt cggcataact
     5401 gcccataatc gcggcacctt ccatcgataa atcgtcagag aaaatcacgc cgtcaaaccc
     5461 cagttcctga cgcaaaacgg ttttcagcca gtagggagaa ccgctcgccg gacgcggatc
     5521 aacatcactg tagatcacat gcgcaggcat aatggcgtcg agtttatttt cgcggattaa
     5581 ggaactgaag accgacatat ctttagcgcg aatctccgct tgtggacgtg gatcgcacgg
     5641 tgtttctttg tgtgagtctg ccgttactgc accgtgtcct gggaagtgtt tcccggtcgt
     5701 tttcattccg gcttcatgca taccatcaat aaaccggctg gcaattgcca gggctttttg
     5761 tggatcggca tgataagaac gctcgccaat cgccgcgctg atatgcccga catccagcac
     5821 tggcgcaaag ctgatatcaa tatccatagc gatcatttcg ctggccatca gccaacccgc
     5881 ctcttgcgcc agtttgccgc cctcttccat tcccaacagc gcagcaaagg attgtgctgc
     5941 cggtaagcgg gtaaaacctt cgcgaaaacg ctgcacgcgt ccaccttcct gatctaccgc
     6001 caccaccaga tgattgcgcg atgctgcgcg gatctggcgc accagttcac gtaactgggc
     6061 aggatcatga tagttacgcg taaagagaat cagccctccc accagcggat gcgccagtat
     6121 ttcacgctct tccgcgtcca gttcgtaacc ttcgacatcc aacattactg gacccacact
     6181 gctctcctta ttgttttatt aatagctgcc gccaggtgtc atcggccagc ctgataaatt
     6241 gttgatcgcc ggtttgtcgc cagcggtact caaaccaccc tcctttgagc atcagcagcc
     6301 agggaaacca tcgcctgacc tgacgccata attgcgccgg ataaatcttc gcgcgagtgg
     6361 catagtcatt gaccaattgc cggtgctgtt cagtattttc cacccacacc gccgccagtt
     6421 ccagcgcgat atcaccatct ccagcatact cccagtcgat gagttttaac cctgatgcgc
     6481 tatgcactaa atttccggcg tggacatcca tatgcaatgg acttaagcgt aaaggtcgtg
     6541 gttcccgcgc tttgcgcaga cgttttaaca ttcgcagcca acccacagtc cgccgcgccg
     6601 gatcgctttg ctgccagtac agttccagta agggcaacag cgttattcgc cagccaaaac
     6661 gtggttgttg atgtagataa tacagcaagc ctgccagttc gttggtatcc ggcaaatacg
     6721 tttttaccgc accgggcaga tagtcgacta ccatccagtc acggagatat aaatgcggct
     6781 tcggtgcaat gcttgcgggt agttgtgata aagcccgata ctggcgcaag aacgcggact
     6841 gcggcgcatc aggatcgtgc ggctgacgca caacaaaacg ctggccctga tgttcaatga
     6901 gaaaactacc gccactaagc ccactattaa acgtcgtgac gggatgaaac tgcgggaaaa
     6961 agcgcgacag caattcgtcg cgcgtgatgg gattattgct gcgaaacggc acctttacct
     7021 gaccagataa tttcgcccgt ctgcaccagc atcagctgca tttgtagggt cggagcgtta
     7081 acgttgccag aggcgcagga gtacagcacg taatgagcgc cgacattgcg ggcaatgcct
     7141 atggctttac tacgggtgcc taaactgtcc tgcggcgaca aacctaactg ttgcttagcc
     7201 atcgacagtt gctgggcgga aaccagggta aatttcccgt tattagccag cgcatttcgc
     7261 agcgtttcgg tcgcttctgc ggcattcagc gaaccgttag tacggttgtt aacgctatca
     7321 accagcagga cgctacccgc agtcaccccg tcagccccaa gcatcttact gaccatcggc
     7381 tgcattgcgc cattccagtc ataatggcga atatgcggcg caggcggtgc cgttcgatct
     7441 tcgtgctcaa ttgggcctgg ctgctgcggg atcgtcggca ccgagggcac tgtggggaca
     7501 ggctgttgtg gctcggctgg ttgttccggc gctggtttca cttcttctac cggtgcaggt
     7561 tcacgttgcc ccacacaccc ggcgagaaac atcgccagcg cggtaatcaa ggcgtagcga
     7621 ctcatttttg tcatcaagat tcaccccttt acaaatatag ataaagtctg actttgtgcg
     7681 cccccagaaa attggcgctg ccgtacagcg ttaccgccga atgtgcggga atggtaacgc
     7741 tgcgtggcct ttccagagga tgcatctcca gccctctggc gtcataccag taaaaacgat
     7801 aatgaacggt aacgggttct tgcctttcgt tataaatcgt tgaggatgct gaaggttgaa
     7861 tatcagacgt cgaaaggacg ggcttttctg cactgatgcc cgcagccaat aaagatgact
     7921 ccatcaccag cgattgctca tcattcaccg gaatttccgg atgtgaacga cagcccacca
     7981 gcagtaacaa caccagagac accagcccaa agcatccttt tctcatcgtt acagaccttt
     8041 atgcgccagc attggtccca gcggacggcc acccaacaag tgcatatgga tgtggtaaac
     8101 ctcttgtccg ccatggcggt tggtattcat gatcagacga tagccatctt cggcaatacc
     8161 ttcttgctca gcaatttttg ccgctacggt gatcatgcgt cccagcgcct gctcatgctc
     8221 agctgacacg tcgttcacag tcggaatgag aatattcgga atgatcagaa tatgcgttgg
     8281 cgcttgcggc gaaatatcgc gaaacgccgt taccagatca tcctggtaga cgatatcgga
     8341 ggggatctca cgacgaataa ttttgctgaa tatagtttct tctgccacga cattttcctt
     8401 tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca cactgcgagt atagagtatg
     8461 gtcgagttaa acctgctctt tcaatcttaa ctcacgattt cttaagcaaa aaatcgtttt
     8521 gctgccgtat atatcgccat tattcccatt tctgcctgcg aatgcgctga aaagacattc
     8581 tctgtttggt ttacacgaca aatatgaatg cgtatatttc tcatttgcat ttgcagcaaa
     8641 attattcgca cactaaatat aaccgctcat caccctcctc cggcatgagc ctgacgacaa
     8701 cataaaccaa gagatttcaa atgctttcaa cacaatttaa cagggataat caatatcaag
     8761 tcatcaccaa accgtcacta cttgccggtt gcatagcact ggcactatta ccttctgccg
     8821 cttttgctgc accagccact gaagaaacgg tgattgttga gggttcagcc acagctccag
     8881 atgatggcga aaatgattac agcgtaacgt ctacctctgc gggtaccaaa atgcagatga
     8941 ctcaacatga tattcctcag tcggtcacta ttgttagcca gcagcggatg gaagatcagc
     9001 agttacaaac gctgggcgaa gtgatggaaa acacgctggg gatcagcaaa agtcaggcgg
     9061 attccgatcg tgctctttat tattcccgcg gattccagat cgataactat atggttgatg
     9121 gtatccccac ctattttgaa tcgcgctgga atctgggcga cgcactttct gatatggcac
     9181 tgtttgaacg cgtagaagta gtgcgtggcg cgacaggact catgaccggg acgggtaatc
     9241 catctgcggc aattaatatg gttcgaaaac acgcgacctg tcgtgaattt aaaggcgatg
     9301 tctcggcgga atacggtagc tggaacaaag aacggtatgt ggcggattta caaagcccac
     9361 tcaccgaaga cggtaaaatc cgcgcgcgaa ttgtcggcgg ctaccagaat aacgactcat
     9421 ggctggaccg ctacaatagt gaaaagacct tcttctcggg cattgtcgat gctgatttag
     9481 gcgatcttac gacgctttca gccggttacg aatatcagcg cattgatgtt aatagcccaa
     9541 cctggggcgg tttaccgcgc tggaatactg atggcagcag caacagttac gatcgcgcac
     9601 gcagtaccgc acctgactgg gcgtacaacg ataaagagat caacaaggtc tttatgaccc
     9661 tgaagcagcg gtttgctgat acctggcaag cgacactgaa tgccacccac tctgaagtcg
     9721 aatttgacag caaaatgatg tatgtcgatg cctatgtaaa caaagcggat ggtatgctgg
     9781 ttgggccata cagtaattat ggacctggct ttgatcatgt cggcggcacc ggttggaaca
     9841 gtggcaaacg taaagttgat gcgctggatt tgttcgctga cggtagttat gaattgtttg
     9901 gtcgtcagca caatctaatg tttggtggca gttacagcaa acaaaacaat cgttcagttc
     9961 atgggccaac atcttcccgg atgaaattgg cagtttctac aactttaatg gcaatttccc
    10021 acaagccgac tggtcatcac agagcctggc gcaggacgat accacacata tgaaatcgtt
    10081 atatgctgcc actcgtgtca cccttgccga tccgctgcat ctgatcctcg gcgcacgtta
    10141 taccaactgg cgggttgata cgctgactta cagcatggag aaaaaccaca ccacgcctta
    10201 cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc tacgccagct atacctctat
    10261 tttccagccg caaaatgatc gtgacagttc aggcaaatat ctggctccaa tcaccggtaa
    10321 caactacgag ctgggtctga aatcggactg gatgaatagc cgtctgacca ccacgttagc
    10381 catcttccgt attgagcagg ataatgtcgc tcagtccacc ggtacaccta tccccggcag
    10441 caacggcgaa accgcctata aagcggtgga tgggacagtc agtaaagggg tggaatttga
    10501 actcaacggc gcaattaccg acaactggca gctgacattt ggcgcaacgc gctatcttgc
    10561 agaggataac gaaggaaacg ccgttaatcc taatctgcca cgcaccacgg ttaaaatgtt
    10621 caccagctat cggttgcctg tcatgccaga gttgacagtc ggcggtggtg ttaactggca
    10681 aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc ttccgcgccg agcaaggtag
    10741 ctacgcgctg gtggatctct tcacccgcta ccaggtgacg aaaaacttct cgttacaggg
    10801 gaacgtcaat aacctgttcg acaaaaccta cgataccaac atggaaggtt ctatcgtcta
    10861 cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa ttctgaataa cacctgtaaa
    10921 aaaggcagcc atctggctgc cttagtctcc ccaacgtctt acggattagt ggttacggat
    10981 gtactcatcc atctcggttt tcaggttatc ggatttagta ccgaaaatag cctgaacacc
    11041 agaaccagca accactacgc ccgctgcgcc cagtttcttc aggccagcct gatccacttt
    11101 agacacatca gcaacgctga cgcgcagacg ggtaatacat gcgtcgaggt tagtaatgtt
    11161 ttctttacca ccaaatgcag caaccagagc cggtgccatt tcgctggtac ctgttgcttt
    11221 tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga tccagtgctt taatcagcac
    11281 gcggaagatg gtgtagtaaa caatcgcata accgataccg acgatcggta acagccacag
    11341 tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga ccgtgcgaga acgacgtacc
    11401 gtcacgcatc cccagaagaa tacagattgg gaatgccagg cctgccagaa tcgcgtggat
    11461 gatgtacagg atcggcgcaa cgaacatgaa gaagaactcg atcggctcgg tgataccggt
    11521 caggaacgag gtcagcgccg cggagatcat aataccgccc actttcgcgc ggttttctgg
    11581 tttagcagag tgccagatag caattgcggc agctggcaga ccgtacattt tgaacaggaa
    11641 gccaccagac agtttacccg cagtcgggtc acccgccata taacgcggaa tgtcgccgtg
    11701 gaaaacctga cctgctgcgt tggtgtattc accaatctgc atctggaaag gtacgttcca
    11761 gatgtggtgc agaccaaacg gtaccaggca acgttcgatg aaaccgtaaa tgccaaacgc
    11821 aactaccggg ttctggtaag cagcccactg agagaaggtc tggattgcag aaccaatcgg
    11881 cggccaaatg aaggacagca caacgccagt aaagatggca gccaggccag aaatgatcgg
    11941 cacaaagcgt ttaccggcaa agaagccaag atactcaggc agcttaatac ggtagaaacg
    12001 gttaaacatg tacgctgcaa tcgcaccgga gataatccct ccgagtacgc cagtatccgc
    12061 caagtgttta gaggcgattt cttcagcagg taaatgcagt accagtggcg caaccacggc
    12121 catggtttta accatgatgc cataggcaac aactgcggcc agcgcggata cgccatcgtt
    12181 attggtaaag ccgagggcga caccgatcgc aaaaatcagt ggcatgtttg caaagacgga
    12241 accgcctgct tctgccataa catgcgatac aacggcgggc agccagctga aattcgcgga
    12301 accgacgccc agcagaatac ctgcgatagg tagtacggat accggcagca tcagcgattt
    12361 accgaccttt tgcaggttag caaatgcatt cttaaacata attgagagtg ctcctgagta
    12421 tgggtgcttt ttttacgttc tcacgcgtgg caagggggga gagcctcgcc gtgtacaggg
    12481 catctaagcg ccctttattt attacacaga gtaaaataat tcagtgccaa tttgtttgac
    12541 ggctatcacg tttcaacttc agatcattgg agaagttgca cattttgaca aaacatatgt
    12601 cacaatattt caaacgcggt tattcaccgc ccaattttcg gcggtgaact ttactcgttt
    12661 tagccattaa ttacgagctt taaaaaaact cattcaacgg atggattgaa ggcgggaagc
    12721 gtcgatgtga aacagacggg cgaagttatc ggtggttacc tgcgccagtt cttcaacggc
    12781 aacacctttc aacacagcca tgtattctgc aacgtcacga accatcgcag gttgattctc
    12841 ttttcctcga tgcggtaccg gcgcaaggta aggtgagtca gtttccacca gtaatcgatc
    12901 cagggggaca taacgcgcag catcgcgcag ttgctccgca ttacggaagg tcacaatgcc
    12961 ggaaaaggag atgtaaaatc cgagatccag taatttaccc gccgtttctc tgtcctctgt
    13021 aaaacagtgt agtacgccac cgcaatccgt cactttttct tcgcgcagaa ttgccagcgt
    13081 atcggcacgg gcgtcacggg tatggacgat aaccggcttg ttcagttcac gaccaatctg
    13141 gatatgatgg atgaaggact cttgctgacg tactttagtt tccggtgtgt aataataatc
    13201 cagcccggtt tcacccagcg ctacaacacc ctcttctgcc gccagacggc gtaaatcttc
    13261 tacatcgtag ggatcattct ggttgagtgg atgcacgcca caagaaaata cgacgttgtc
    13321 acgttcgcct accagatccc gcatatgtaa ataacccggt aatgttgtgg cgactgccag
    13381 acaaaatttc acatcgcgtg cggcggcttt cgccagaacg tcatccacgt ccttatgcaa
    13441 agattcataa tccagaccat cgagatggca gtgtgagtcg actaaaaaca tgatgtctct
    13501 cttaaagatg aggaaccggt aacacaacgc ccggttgcag gtaatgctca atacgcagta
    13561 aaagatcggt gatgagaagc tcgcggttga tgcctgtgac agacattaac tgttcacgaa
    13621 tgtggcaaac atcccccagt atagcctgca ggcacgaggg agaaagatgg tttgccagtt
    13681 cggcgaccag gcccggcaca tcaacattgg tcacctgcgc agcgccatga tggcgtttta
    13741 gcgcatccat cagcaacgtt gccagccagt gtaaacgcgc cggagcttgt tcatgattaa
    13801 gggccgccag cagcgaatac cagtcgcccg atggcacgct atatgccaac gcctgacaca
    13861 atgtttcacg agcctgccag ttatctccct gaaacaacgc cagtgccgcg ccaggcgaac
    13921 cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga cattgtcact tcgcgtgaaa
    13981 gccaagtcac ggcgtactgt tctggaggcg gcgcaaggta atgtaaccga caacgactac
    14041 gtaatgttgc cagtaaacgt tcaggctcgc gggtagccag gaaaaaccag gtttctgctg
    14101 gtggctcttc aagcgttttc agcaatgcgt tagccgcggc gtcggtcagt aaggcagcat
    14161 cggttaccca gacaactttc gcaccaccta ggcgtgcgtg ctcattcagc ttttcggtga
    14221 cctcacgtac cgcatcaatg cccagcgtat ttttcccttt ttcgggagcc agggtgtagt
    14281 aatcgggatg cgtgccagcc tgcatcaact gacatccacg acagtgaccg caacttttgt
    14341 ggccctgcgg ttgttggcag agtaaataac ggctcagggc gtagattaaa gcatcatcgc
    14401 ccatgcccgg taacgcctga atgagtagcg catggtgacc tcttccggcc tgatagctgg
    14461 ctaccagttt ttcgaaatca ggtcgtaacc atggatacca tctcatgcgt ccaactcctt
    14521 cacccagtgg gtcacggtag tgcggattgc atccatcacg gcctccagcg gctgggtggc
    14581 atcaatggta tgaatacttt tatcttgtgc agccagttcc agatagcggg cgcgggtgcg
    14641 attaaagaaa tcgaaagact cttgctcaat acgatccagc tcgccgcgcg cacgcgcgcg
    14701 ttttaagcca acttccgggg taacatcgag atagagcgtt aagtctgggc gaaaatcccc
    14761 gagaacagca tcacgcagtg ttgccagcat atgttggtca ataccacgtc ctccgccctg
    14821 atacgcctga gtggaaagat cgtggcgatc gccaatcacc caggtgccgt tagccagcgc
    14881 aggtttgatg accgtttcta ccagttgaac gcgcgcggca taaaacatca gaacttcggc
    14941 tttatcggta atgacttcat cgcctaccga tttgatatcc agcaccaggc ttcttaactt
    15001 ttcggcaagt tgcgtaccgc caggttcccg agtgaaaacc atgtcgcgga tacccagttg
    15061 ctcgagcgtc tcaaccacca cattacgcgc ggtagttttg cctgcgcctt ccagcccctc
    15121 aatgacgata tacttactgc gcattttttt ccttaagcac tttcagataa tcctgcacag
    15181 acttgttatg actggcaaga ttggtattaa acgtgtgacc acctttacca tcggccacaa
    15241 aatagagata cggcgttttt gccggatgcg cagcagcctt cagcgaatcc gcccccggcg
    15301 tagctatcgc acccggcggc aaaccggtaa tggtataggt gttatacgct gtcggcgttt
    15361 ccaggtctgc acgagaaagt ttgccattat aacgctctcc catcccgtaa atcacggttg
    15421 ggtcggtctg caggcgcata ccaatgcgta aacggttgat aaataccgag gcaacctgat
    15481 cgcgttcact ggcaacggcg gtttcttttt cgataattga tgccatcgtc accagctgat
    15541 ttttatcttt ataaggcaga ccgtccgcac gcccttccca ggcgctatcg accgctttca
    15601 ccattttctt gtgaactcgc ttgagtaacg cgacatcggt ggtattggcg gtatacatca
    15661 aggtgtctgg ccagaaccaa ccttcaatcc actccgggtt ttccagttca agtgcctgcg
    15721 ctacggtggc gtacttatcg tcactcagcg tgtgcttgat atacggagcc tcacgcaatt
    15781 gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg cagcgggaac tgtgcttctt
    15841 taccgctttc caacaatttc agcatctcgc gcacggtcat ctgcggtgta aagcggtaag
    15901 tcccggcttt aaagtgagaa agatccggtt cgatacgcag cagccattga aaaacccgtg
    15961 ggcgattgat gatcttatca gcataaagct gttcaccgag cgccagacgt ccggtccctg
    16021 gcttcagggt aaatatcgtc tcttctttga taagcaattt gctgtcggca agatggcgaa
    16081 ccttccagac gcccacacca gcggcgatac ccagtaccac cagcaataac aagataatca
    16141 ataacacttt tttcatgact aattcgggcg ctcacaaagt ggggctaaat attcatataa
    16201 cgttgctgac gaaaaggtga catcgccaca ggcacgtacg ggcatcactg gcattaacgc
    16261 attacaaata accatctcat ctgcctgcaa cgcctcctcc agcgaggctt gcacttcgac
    16321 aagctgataa gaggattgtg ccagtaaacg gatacagaat tgtcgcataa tgccgttaac
    16381 acctgcctga tccagtcgcg gcgtataaac tacattgccc ttccgccaga acaaattagc
    16441 cgcacagcat tccgtaaccc acccttcgct gtcaaggacc agcgcctcat cagcgtttgt
    16501 ctgctcaaga tgagagcgaa tcaatacttg ctcaagccga ttaagatgtt taatacctgc
    16561 aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc aacgtcatcc cttcgttacg
    16621 caaacggtcg taatgtgcag gataagccgt aacggagaga atccgcgttg ctggtccgct
    16681 gttcaatgtg ctgtaccctc gcccgccact accgcgactg atcacgactt tcagcacacc
    16741 attttgctgt tctgctgcca gcgttttcat ctcttgttca agctgaggcc agaagtcaca
    16801 ggaaatcatc aaccgctgac aagcatcctg tagtcgcagg atatgcgccg ataacaaact
    16861 gactttaccg tcgataactc tggcggtggt aaaacaacca tcaccaaact gcgttgcccg
    16921 atcgcttact gccagcgatt cctgcttata accgttaatt aagaacatac cggctcctta
    16981 tcagagaatt gttagtgtgg cagcatgttc actacggaac aagtcggaat aaaagctaag
    17041 aaaaaaggcc cgcaagcgga ccttttatat gggtggaaaa tgacaactta gatcttttta
    17101 aagatcaaag aaccattagt gccaccgaag ccgaaggagt tacacagcgt gtattccatt
    17161 ccgctgacct gacgtgcttc atgcggtacg aaatccagat cgcaaccttc atccgggtta
    17221 tccaggttga tggttggcgg aacagcctga tcgcgcagcg ccaggatgga gtagatagat
    17281 tctactgcac ccgccgcacc taacaggtga ccggtcatag atttcgtgga gcttaccaac
    17341 acacggcttg cagcttcacc gaagatggtt ttcaccgcct gcgcttcagc tttatcgcca
    17401 gccggcgtag aagtaccgtg cgcgttaacg tagccaatct gactcgcttc aatgcctgca
    17461 tcacgcagag catttgccat tgccagagct gcgcctgcgc cattttctgg cggtgacgtc
    17521 atatgataag catcgctgct cataccaaag ccgacgagtt cagcgtaaat tttcgcaccg
    17581 cgttttttcg cgtgttcgta ctcttcaagt accagcatac cggcaccatc gcccagtacg
    17641 aagccatcac gctctttatc ccacgggcgg ctcgccgctt gcgggttatc attgcgggta
    17701 gataatgcac gtgccgcgcc aaaaccacca acgcccagcg gcgtactggc tttctctgcg
    17761 ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa tacgcgcagc atggccaatg
    17821 ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc ttgggccacg caggccatac
    17881 atgatagtca gatgacctgc caccatgttc acaatcgttg acggaacgaa gaatgggctg
    17941 atcttacgtg gaccaccgtt catcagagat gtgtggtttt cttcgatcag tccgaggccg
    18001 ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt tctcttccgt tatttcaagg
    18061 ccagaatcct gcatggcctg aacgccagcg acaattccat attgaatgaa ggcatccatc
    18121 ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa aatcctttac taagccagca
    18181 aatttcgttg cataggcgct agtatcgaaa tggtcgatta ggctgatgcc actctgaccg
    18241 gcaagcagag ctttccaggt agactctacg gtattgccga caggagacaa catgcccagt
    18301 ccggtcacaa ctacacgacg cttagacacg tttgtcctcc aaggagggaa aaatgattct
    18361 agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg cctggagatg ttcacttacg
    18421 cctggtggcc gttgatgtaa tcaatggcag cctgaacggt ggtgattttc tcagcttctt
    18481 cgtccggaat ctcagtatca aactcttctt ccagagccat taccagctca acggtgtcaa
    18541 gagaatccgc gcccaggtct tcaacgaaag aagcattgtt ggtaacttct tcctgcttaa
    18601 cgcccagctg ttcgccgata attttcttaa cgcgttcttc gatagtgctc atactcttaa
    18661 atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt gtataaaatg ttgaaaaatt
    18721 tgcaactaaa tcccggcagg tcttaccacg attttacgtt attttgaggc cttacccccc
    18781 aataacgcaa atcattttgc actaattatt actcataacc acgcagactg cgcgcaacat
    18841 gagcattttg tgcaaatcgc ggtcagacca tgtacatccc gccgttcaca tgcaaagttt
    18901 cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc aaccgcgttg gcgatttcct
    18961 gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat acccgcacgc tggtcatcgc
    19021 tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc aacaacgttt acagtaatac
    19081 cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc gatcaagccc gctttcgccg
    19141 cagcgtagtt ggcctgaccg ccatttccca tggtaccaac cacagaaccg atagtgataa
    19201 tacgaccatg acgctttttc atcatagcgc gcattaccgc ttttgacaga cggaaaacag
    19261 atgaaaggtt agtttcgata atatcgttcc actcttcatc tttcattcgc attaacaggt
    19321 tatcacgagt gataccggca ttattgacca ggatatccac ttcaccaaat tctgcgcgaa
    19381 ttttttccag aacagattcg atagatgccg ggtcggtcac attcaacatc agacctttgc
    19441 cgttggcacc taaataatca ctgatcgcct gagcgccatt ttcactggtc gcagtgccaa
    19501 taactttcgc gccacgggct gcgagcgttt cagcaattgc gcggccaatt ccgcggcttg
    19561 caccggttac cagtgcgatt tttccttcaa aattcatgat tttcctcttt taaagctcga
    19621 gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc ggtcagggtg tcgacaatgc
    19681 gtttcgtcag gccagtaagc accttgcccg ggccgacttc atagagatgt tctacgcctt
    19741 gcgctgccat gtactcaaca gacttcgtcc actgaaccgg gttatacaac tgacgtacca
    19801 gtgcgtcacg gatggcatca ccattggttt cgcatttcac atcaacgtta ttcacaacag
    19861 aaactgttgg tgcgttaaag gtgattttcg ctaattctac tgccagtttg tcggctgctg
    19921 gtttcatcag cgcacagtga gacggtacgc tcactggtaa cggcagcgcg cgttttgcgc
    19981 cagcagcttt acaggcagcg ccagcacgct caaccgcttc tttatgaccg gcaataacca
    20041 cctgtcccgg agagttaaag tttaccggag aaacgacctg accttctgca gcttcttcac
    20101 acgctttcgc aataggcgca tcatccagac cgatgattgc cgccatagcg cccgtgcctt
    20161 ccggtacggc ttcttgcatg aacttaccgc gcatctcaac cagacgcacc gcatcagcga
    20221 aatcaatcac gccagcgcaa accagcgcgg agtattcccc caggctgtga ccggccatca
    20281 ttgccggtgc tttaccgccc tgctgctgcc atacgcgata cagcgcaaca gatgcagtca
    20341 acagcgctgg ctgggtttgc caggttttat tcagttcttc agctgacccc tgctgggtca
    20401 gcgcccacag gtcgtagccc agcgccgcag aagcttcagc aaacgtttct tcgacaattg
    20461 gatagctcgc cgccatatca gccagcattc caacggtttg agaaccctgt ccagggaaca
    20521 caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa acgaaccagc gcagagcccc
    20581 aagtgaatcc accgccaaag gcttcaagca gaaccaactg ccccggctta atgcgcccgt
    20641 cgcgtacagc ttcatccagc gcgcacggga cagaggccgc agaggtatta ccgtggcgat
    20701 ccagcgtcac cacgacattg tccatcgaca taccgagttt tttcgccgtt gcactgataa
    20761 tacgcaggtt agcctgatgc ggaaccagcc agtccagttg agaacggtca agattattcg
    20821 ccgccagcgt ctcatcaacg atgtgcgcca gttccgttac cgcaaccttg aagacttcgt
    20881 tgcccgccat cgtcagatga attgaattct ctggattcac gcggtcggcg tttggcagcg
    20941 tcagcaattc accataacta ccgtcggcat gcagatgggt ggaaatgatt cccggctctt
    21001 cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa aataataata gtcccacgat
    21061 ctgttggatc gcaggtgcgc gccagtacat cggaaccgac gaccagagca tacttcaccg
    21121 ccccagattt cacgtattga tcggctacgc ttaatgcata ggtgaaacct gcacaggctg
    21181 ctgcaacgtc aaatgccggg caacctttaa tgtccaacat gctttgaatc tgacaagctg
    21241 cgctcgggaa agcgtgcgta gcagaagtcg ttgccacaac gatcaggcca atctggtctt
    21301 tctcaatgcc cgccatctca attgcgcgtg tcgccgcttc aaagcccatg gttgaaacgg
    21361 tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt acgagtgaca atccactcgt
    21421 cagaggtgtc caccattttt tccaaatcgg cgtttgtccg cacttgttcg ggcagatagc
    21481 tgccagtacc aataatcttc gtatacatgt acgctcagtc acttttcggt tatataccgt
    21541 cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc gcagagttcc gcttttgcca
    21601 ccgtccagca gctcaaaacc agctgggtat acagattcca ggcgagcggc aattcgctga
    21661 ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg cgaccgcaaa agctcgctga
    21721 ttggctgcac catgactttt tatcaccgtg ccgcgcaatc ctaacagaca ggcgccgtta
    21781 tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc tcttttgtag ccaacgcttt
    21841 aataacagta gccaccacga ccgttttttc ccttcaccct gagatttcag cagagaaagg
    21901 aacatcctga caacaccttc catcgtcttt aacgtgacat ttcctgtaaa gccgtcacaa
    21961 accagcacat ctgtcttgcc agttaacaac tcattggctt caagatagcc gatataattg
    22021 atagaaggga ttgttttaag caccgctgag gcatcccgaa tactgtcaag accctttact
    22081 tcttcttcac caatattgag caacgccacg cgaggattgg gaatttccac cacctcttca
    22141 gccagaactg agcccataat ggcaaattgc accaacattg tgctgtcaca atcgacgttg
    22201 gcccctaaat cgaggaccac cgttttgccc ttttgctgat gtggtaatac cgtcaccagc
    22261 gccggacgct caatcccctc caggggcttg agtaataatt ttgccagccc catcagtgcc
    22321 ccggtattac cggcactgac acaggcttgc gctcgacctt ctttcaccag ctccagggcc
    22381 atacgcattg aactcccacg gctggcgcgg atagcttgcg aaggccgggc atcactggcg
    22441 ataactgact gcgcaggaat aatctgcaga cgcgaacgtt gttcaaagtc agctttagca
    22501 agtaatggcg tgatggcgtc gggattgccg actaaaagaa gagtgagttg cgaattagaa
    22561 ttcagtgcct gcaatgctgc aggcactgtc acggaagggc caaaatcccc tcccatgaca
    22621 tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc ccggtttggt ctttccccag
    22681 ttgcctgggg aaaatcctca ctaagcttca tcacgcagac gcgtgattac ttagcgatga
    22741 ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg acgcaggtgt ttttcaccag
    22801 aagttttgtc tacagacagg ctggtgactg cggtcagcgc gtcatgggaa cgacgcatgc
    22861 cacgtttgga acgggttggt ttattctgtt gtacggccat ggaccttact cctcaattac
    22921 ggtttatccc cgatccaacg gggagcacca attacttacg ctttaagctg gctaatacgg
    22981 caaatgggtt tggcttttgc acttcttcag gcagttcacc aaagaccatg tccgcttcgg
    23041 acacttcaca gtgttcagaa tcatgcaccg gaactaccgg caaggcgagg atgatttcat
    23101 cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt aacctcaatc ggttcatacg
    23161 cttccggcag tgcttcagcc tgttcgtctg aacgcacagg actaaaacaa tacgttgtgt
    23221 agacctgatg agtaaacggc ttcccgcaac gctgacactc gagcgttacc gtcaccttcg
    23281 catcgccgtt taacactgcg agacgttggt tatcgatagc gaacgacatg gagcattcca
    23341 catcactgtc cacactgact acggattcgg cgacgcgctc aacctgatca ggggtataga
    23401 taccctggta atcaaggcgt ttttgagccg tacgaaccgg atcgagagtc aggggtaatt
    23461 ttaccttttg catagggcgc gcatattaac tttgtaacgt catagagtca aagaaaaagg
    23521 cagcctgtgg ttgccttttg ccaataattc gcacacattg cgggttactg ctttattttc
    23581 gttcagtgct gataacgaac atcaacctgc aaaacgaagc gcatagttta aaatggctat
    23641 catcaatacg ctatatctga tggaaaaaaa tatgcctaaa cttattttag cctccacctc
    23701 gccatggcgt cgcgctcttc tggaaaaact gcaaatctct tttgaatgtg cagcacctga
    23761 ggtcgacgaa accccacgca gcgacgaatc accgcgacag ttggtgcttc gactggcaca
    23821 agaaaaatcg caatctctgg cgtcacgtta tccggatcat ttaattattg gttcagacca
    23881 ggtatgtgtt cttgatggcg aaatcaccgg taaaccgtta acggaagaaa atgcccgtct
    23941 gcaattacgc aaagccagcg gcaatatcgt caccttctat actggactgg cgttgtttaa
    24001 ttcggcgaat gggcatctac aaacagaagt ggagcctttt gacgtccatt tccgtcacct
    24061 aagcgaggcg gagattgata attacgtgcg taaggagcat cccctgcact gcgcgggtag
    24121 ctttaagagt gaaggatttg gcattacgct gtttgagcgt ttagagggac gtgaccctaa
    24181 cacgctggtt ggtttaccgc taatcgcgct gtgtcagatg ttacgtcggg aagggaaaaa
    24241 cccgctgatg ggatagttgt ttgcgccgga tgcggttacg tcgcatccgg caatcggtgc
    24301 aggcggcctg atgcggcgta aacgccttat caggccaacc gcccattatc aggcttatat
    24361 gcttgtatta gcgcgcgtta cgcagctttt gcaaacaacg cttcaaacct tcatccatcg
    24421 gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt aaacttcaac gctgcggcgt
    24481 gcaggaacag acgatttaat cccgtgcctg cttcagtgag ctgtctgtca aattcacggt
    24541 caccgtagcg atcgtcaaag gcaatcggat gacccgcata ctgtgtatgc acacggatct
    24601 gatgagtacg tccggttacc gggctacaac gcaccagggt ggcaaatgca tagcgttctt
    24661 ccactttaaa gcgtgtttcc gacggtttgc cttcctgact gacacgcacg atacgttcgc
    24721 cgctttgcag aatatttttc aataacggcg cttgaacgct cttcacatgc gactgccact
    24781 gaccgcgcac cagcgccagg taatcttttt gcattccttt ttcacgtaat tgctcatgca
    24841 gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac acctgaagtg tcccggtcga
    24901 gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa ccttcgataa
    24961 cgccgaagct taaaccactg ccgccatgta ccgccgtacc ggaaggttta ttcagcacca
    25021 ggatatgatc atcttcatat aagatgacgt ccgccagcgc cgccaccttt tgcagatgtg
    25081 gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg tgggatacgc acctcatcac
    25141 ccgcttcgag tttatattca ggcttaatac gttttttgtt cacccgcact tcacctttac
    25201 gcaaaatacg gtaaatcata ctttttggta cgcctttcaa ttgggtacgc aaaaagttat
    25261 cgatacgttg ccccgcttcg tcagcggtga tagcaacaat ttttacggat ggagtctctg
    25321 ttttcatggt gggcgattct aattagccaa caggattcgc gccactcatt tttctatgct
    25381 tatttttagt tttgcacctt attacttcac tacgtgatca ctttattgat ggttattaaa
    25441 ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg gtaaaagtca
    25501 tcttgctata acaaggcttg cagtggaata atgaggccgt ttccgtgtcc atccttgtta
    25561 aaacaagaaa ttttacggaa taacccattt tgcccgaccg atcatccacg cagcaatggc
    25621 gtaagacgta ttgatctttc aggcagttag cgggctgcgg gttgcagtcc ttaccggtag
    25681 atggaaatat ttctggagag taatacccag tctgtttctt tgataattgc gctgtttttc
    25741 cgcatgaaaa acgggcaacc gacactctgc gcctctttga gctgacgata accgtgaggt
    25801 tggcgacgcg actagacacg aggccatcgg ttcacacccg gaaaggcgtt actttgcccg
    25861 cagcttagtc gtcaatgtaa gaataatgag taagttacga tgaaaagaat gttaatcaac
    25921 gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg ggcagcgtct gtatgacctg
    25981 gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca tctacaaagg taaaatcacc
    26041 cgcattgaac cgagtctgga agctgctttt gttgattacg gcgctgaacg tcacggtttc
    26101 ctcccactaa aagaaattgc ccgcgaatat ttccctgcta actacagtgc tcatggtcgt
    26161 cccaacatta aagatgtgtt gcgtgaaggt caggaagtca ttgttcagat cgataaagaa
    26221 gagcgcggca acaaaggcgc ggcattaacc acctttatca gtctggcggg tagctatctg
    26281 gttctgatgc caaataaccc gcgcgcaggt ggcatttctc gccgtatcga aggcgatgac
    26341 cgtaccgaat taaaagaagc actggcaagc cttgaactgc cggaaggcat ggggcttatc
    26401 gtgcgcaccg ctggcgtagg caaatctgct gaggcgctgc aatgggattt aagcttccgc
    26461 ctgaaacact gggaagccat caaaaaagcc gctgaaagcc gcccggctcc gttcctgatt
    26521 catcaggaga gcaacgtaat cgttcgcgcc ttccgcgatt atttacgtca ggatatcggc
    26581 gaaatcctta tcgataaccc gaaagtcctc gaactggcac gtcagcatat cgctgcatta
    26641 ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg gcgagatccc gctgttcagc
    26701 cactaccaga tcgagtcgca gatcgagtcc gccttccagc gtgaagtccg tctgccatcc
    26761 ggtggttcca ttgttatcga cagcaccgaa gcgttaacag ccatcgacat caactccgca
    26821 cgcgcgaccc gcggcggcga tatcgaagaa actgcgttta acaccaacct cgaagccgcc
    26881 gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg gcctgattgt tatcgacttc
    26941 atcgacatga cgccagtacg ccaccagcgt gcggtagaaa accgtctgcg tgaagcggtg
    27001 cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc gctttggcct gctggaaatg
    27061 tcccgccagc gcctgagccc atcactgggt gaatccagcc atcacgtctg cccgcgctgc
    27121 tccggtaccg gtaccgtgcg tgacaacgaa tcgctgtcgc tctctattct gcgtttgatc
    27181 gaagaagaag cgctgaaaga gaacacccag gaagttcacg ccattgttcc tgtgccaatc
    27241 gcttcttatc tgctgaatga aaaacgttct gcggtgaatg cgattgaaac gcgtcaggac
    27301 ggtgttcgct gcgtgattgt gccaaacgat cagatggaaa ccccgcacta ccacgtgttg
    27361 cgcgtgcgca aaggggaaga aacgccgacc ttaagctaca tgctgccgaa gctgcatgaa
    27421 gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac gtaagcgtcc tgaacaacct
    27481 gcactggcaa cctttgccat gccggatgta ccgccagcgc caaccccagc tgaacctgcc
    27541 gcgactgtcg tagccccagc accgaaagct gcaacggcaa caccagcagc tcctgcacaa
    27601 cctgggctgt taagccgctt cttcggcgca ctgaaagcgc tgttcagcgg tggtgaagaa
    27661 gccaaaccga ccgagcaacc aacaccgaaa gcagaagcga aaccggaacg tcaacaggat
    27721 cgtcgcaaac ctcgtcagag caaccgccgt gaccgtaatg agcgccgcga cacccgtagt
    27781 gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc gtaatcgtcg ccaggcacag
    27841 cagcaaactg ccgagacgcg tgagagccgt cagcaggctg aggtaacgga aaaagcacgt
    27901 accaccgacg agcagcaagt gccgcgtcgt gaacgtagcc gccgccgtaa cgatgataaa
    27961 cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag agcaatctgt tcaggaaacc
    28021 gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta aacagcgtca gctcaatcag
    28081 aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag tcgcaccggt ggttgaagaa
    28141 actgccgctg ccgaaccaat tgttcaggaa gctccagctc cacgcacaga actggtgaaa
    28201 gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc aagaagagaa caatgctgat
    28261 aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct cgcctcgtca cctgcgcgtg
    28321 agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc caacccagtc gccaatgccg
    28381 ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca aagtctggat ccgctatcca
    28441 attgtacgtc cgcaagatgt acaagttgaa gagcagcgcg aacaggaaga agtacaagtg
    28501 cagccgatgg tgactgaggt ccctgtcgcc gccgctgtcg aacctgttgt tagcgcgcca
    28561 gttgttgaag aaatggctga agtcgtagaa gcccccgtac cggttgccga accgcaaccg
    28621 gaagtggttg aaacgacgca tcctgaagtg attgccgccg cggtaactga acagccgcag
    28681 gtgattaccg agtctgatgt tgccgtagcc caggaagtcg cagaacacgc agaaccgatg
    28741 gttgaaccgc aggaagagac ggcagatgca gatattgaag aagttgccga aactgctgag
    28801 gttgtggttg ctgaacctga agttgttgct caacctgccg cgccagtcgt cgctgaagtc
    28861 gcagcagaag ttgaaacggt ggccgcggtt gaaccagaga tcaccgttga gcataaccac
    28921 gctaccgcgc caatgacgcg cgctccggca ccggaatatg ttccggaggc accgcgtcac
    28981 agtgactggc agcgccctac ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg
    29041 gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta ataattagct
    29101 caaagtaatc aagccctggt aactgccagg gcttttttat ttcatgtttg aatcaacata
    29161 ttaaaattca tcaaatggca tccttgctaa ccaacaatgc aaaataggca aaaaaaatgc
    29221 ccgccccggc gatcccagag cgggcagaca aaacataccc agtttcatga tatgttttaa
    29281 agcgagctta tttgttgagc tggaagagcg acaatccctg catatcggta aatgctttat
    29341 acgatgcctg caatgccgtt tgctgcatga tgtaagatga aatagttgca ttccagtcca
    29401 catcaaccag atcgctcatc tgctgcgttt gccctaaagc gcgatcgcta cctaaggaat
    29461 ccagcgactc cagtttgttc agctgcgtgc ctaattccgc gcgaacggta aggacattat
    29521 tcagcgagtt tttcagtcca cggttggttt tatccaatgc cgcagcggcg atttctttat
    29581 ccgcttcgct atccgcgacc ggcgttttca gggctgcgat ggcgctatcc agcatggcaa
    29641 aaagattggt ttcagaagcg ctaccgtctg gttccgctac cgcgttactg gtaatactgt
    29701 cgaaaatttt gtcgcccgta tgtcctatca ccatcgaacg cgaagcatcg acctgttgct
    29761 taatactttc tgttccgcca acataatcac cgttagcctc actaaacggc gcagtctctg
    29821 ttttatatgt agcgcccatt accgtcagtg gtgttcgcca gattcagcaa ctggtcacga
    29881 agcccctgaa tatccgtagc cagcgaggcc cggtcatcgt cactcaaggt gccattgctg
    29941 gcgtagacaa ttttttcctg agcattctgg atagcagtgg tgacctggct aagtacactc
    30001 tcttccagtg acactttttg agtggcgaaa gtacgtgcca gcgtgtactg gctgttttgc
    30061 gcctgtgcct gggagagaac aacagcttgt gatgcagcaa tgggatcgtc tgaagggtta
    30121 acgactcgct tacccgtcga catctgctcg ccgtacttca tccattctgc ctgagaattg
    30181 gtgatgccgc gcatgttttg ctggtacatc atctgggtac tgaaacgcat tttatctccc
    30241 cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga acctgcgcat
    30301 tcgccaggta atactgctga aaacgttgca gatttccgta ctcctcatcg agattgacac
    30361 cggaaatcga ctgctgctga ttggaaagct gcgtcaccac attaccttgc gtggtgctac
    30421 tggttttcaa cgtcgcggtt ttattaccga tatcactcac taacgaggca taagcgtcgt
    30481 taaaggattt cgcaccgccc accgttttac tgttgctttg cagatccagc agggcctgac
    30541 cgttgcggtt gtcgctatca cccgcatctt cttcgctcgc catcgcgatt ttcgcttcgt
    30601 cggtgattaa tacatccatg ttgacgatgg cgtcacttac tggtttcagc gtgaagctgt
    30661 cgttaacggc aggcgttcct gtaaacgtca actccagacc atcaaatgcc actttaccgt
    30721 tggcatctgg tgtcgccgta aaagtggtat tgctggcaag gcgggtgacc tgccactgat
    30781 tattatcgaa cgagattttg taatctgtcg ccagtaccac ggaggcatca gttaccgtgg
    30841 caccgatcgc aacgtcacct ttgtttttcg tgttttgcag aaccgcgggc ttaccgatag
    30901 taaagaaatc ttcaccagca tcgccgttag catcaaatcc ggctttgtgt tggctgttga
    30961 aagcctcggc aaatgccagc gccagttgtc caagcgtatt acgcgtctgg tccagatcct
    31021 gagaacggaa tgtcagaatg ccgcccagcg acccggtatt cagtaatttc tccgggatct
    31081 caatattgcc tgccgtccca tcaacgtaag cgacagtcgt acgagaaggg tcggcgctgg
    31141 aaggaactgc cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa ccattggcat
    31201 cgtgatgtta taagtaccgc catcctgaac gctgacttct acgccaacaa tctggtttaa
    31261 ttcgctcacc agttgatcgc gttgatccag cagattgtta ggtgacgccc ctgcccccac
    31321 gcctgtcagg cgcgagattt gatcgttcag gctggcaatt tgtttagcgt aattgttgat
    31381 ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc gcagatattg
    31441 atcggtggtt ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc
    31501 cgccgggtct tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat cctgcatctg
    31561 tgttgccagc gaagaggtac tggtggagag catattgtcg attttcgaca tctgctcata
    31621 gcgggcggtc aggccgctac tttgcgtctg cgcagcacgt aactggttgg taataaacgt
    31681 atcatactca cgctgcacac cagaaacgta gacgccattg ccaacccagc cgccagcgcc
    31741 caacgtgcta ttggcctgcg ccataatagt ggtttggcgg gtatatccgg caacgttata
    31801 gctggagata ttattacttg ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat
    31861 ggcgttattg atcaagctgg acatggaggt tccttataag ccttcaatta cagagtatta
    31921 tcggcagcga cccgccggac ttgagttatt cagaacagat tatcaatgtt catgctgtag
    31981 gttttgctca ccttgtcgct tatcgatttc atctgctgga tcatgttggt gagtttacgg
    32041 gcatagtgag gatcggtagc atagcccgcg tcctgtagga cctgcgcccc ctgttccgca
    32101 ctcgcggcgg tcgtcacggc ggcgtagcgc gggttacgtg ttaacagccc aacgtaatcc
    32161 gacaatgctt ccagatacga gctgtagacc cgaaacttcg cttttacttt cttcgcttcg
    32221 ccgttttcat attcagtcgt ggtgatttca gtgacttgcc ctttccagtt gccagaggct
    32281 ttgacaccaa acaggttata gcttggctcg ccgttttcgc ggcggatttg ccgttgcccc
    32341 caaccagatt caagcgcagc ctgagcgagg atcaaatgat gtggcacacc gctttgctgg
    32401 cttgccagtt gggcgggcag cgagagttgc gcgaggaatg ctctactgtc acccggcagc
    32461 gaatcatcgt agttacgtgg tacggccttt tgcaccagct gcgaaagcgt ctggttttga
    32521 taacgcacca cggtttcgag cgggaatttc atcggtgctg ctggcgtgga ctcctctggc
    32581 aatggttgtt ctggcgtcat ctgtttaacc atcatctctg caagccccag acctttgccc
    32641 gccgtcatct gttgggcaat ttgctggtca tacatactgg tatacagacg agtgtgctcg
    32701 ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat catctgcacg
    32761 aacatccctt ccacctgacg ggccaccgga cggatatttg ccgccggatc ttcgctcgct
    32821 ttcgccttta gttcgttgag tgattgcgca tcccaggccg cacttgccag tagtttgctg
    32881 tcgctgatca tcagatgatt tccagttttg cccgcagaca tcccgcactt tgcattgatt
    32941 gcaaaataga catcagatcc atcggcgtag cgcccagcgc attgagcgca cgcaccacgt
    33001 tattgaggct ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg cgtaaatcga
    33061 tctgcgtttg tggagtaaca acagtctgtc caccaccaaa cggtgtatct ggctggctga
    33121 cattggcctg acgattaact gttactgaga gatttccctg cgctaccgcg cagctgtcga
    33181 gggtcacttc gcgattcatc accaccgaac cggtgcgcga gttaatcact actttagcgt
    33241 cctgcggggt gacattaacc tgcatattct ggatatcggc aaggaaacgg acctgggaac
    33301 tgttgccact tggtacgcgc acctgaatag tccgcgcatc taacgcggtg gcgctgccat
    33361 atccacgcac gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa tcttcgtcgt
    33421 taagttgcaa attaagggta ttcccgacgc caaactggct gggcaattca cgttcaataa
    33481 ccgcaccatt ggtgatccgt ccaccgttca gttggttaac ctgaacactg ctaccgcctg
    33541 cggaggctcc tgcgccgcca accagaatat tgccctgcgc cagcgcatac acctgactgt
    33601 caacgccctt aagcggagtc atcaacaacg tacctccacg caagcttttg gcattaccca
    33661 tggaagaaac caccacatcg atggtttgcc cctgacgtcc aaacggagga agtgacgctg
    33721 tcaccattac cgcagcgacg ttttttagct gcatattggt gcccgtcgga acggtaattc
    33781 ccagctgtga gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg gttgtctggt
    33841 caccagtgcc atccagcccc actaccaagc catagccaat cagtgagttt tgccttaccc
    33901 cctgaacact ggtgagatcg cgaatacgct cagcctgagc cgccgtcgta accagtagaa
    33961 gaattaatgc agagagaaat ttaatcacca cagcctcact tacattggcg acaggttaag
    34021 gaagaaacgc tgcaaccagc ccatattttg cgcttcgtta atgtagccat tgcctacgta
    34081 ttcaatacgc gcatccgcca cctgagtaga cggtacggta ttgctgccgc tgatagtgcg
    34141 tggattaacc acgcccgaga agcgaataaa ttcggtaccc tgattaatgg caatctgttt
    34201 ttcacccacc acatgcaggt tgccgttgac cagtacctgg tcaaccgtca ccgtcaacgt
    34261 gccgctaaag gtattgctgg cattggcccc gccctttccg ttgaacgtgt taccaccgga
    34321 ggcttcgaca tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg gcacagtatc
    34381 aaagccaaaa ttagttttac cgtcacggct ggcattcgca gaggagcttt tgctggcgct
    34441 gacgttctcc tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc gacgatcttc
    34501 aaacagcggt tgatagccat agttaatcgg ctgagcagac tggaaaatag aaccgttggc
    34561 gacgggcgtc ggaccgggaa ccggttgtgc actggtcgcc ccctgcacca gcggcgtgga
    34621 gggtatccag gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg cataagtatg
    34681 cgcagcgttt ttttgcatgg ctatcatctt caaaaatcag taaaccggtg aacctgccac
    34741 cggttatgcc ttagagttgc gtcagttttt gcagcatctg atcggtggtg gacaccgctt
    34801 tactgttgat ttcgtaagcg cgttgcacct gaatcatatt gaccagttct tccgccacgt
    34861 tgacgttaga cgtttcaaca tacccttgat acagcagtcc cgcgccgttc aggcccggcg
    34921 tgctttcgtt cggtgcaccg gaggattgcg tttcggtgta gaggttttcg ccaatgctct
    34981 ccagtccggt atcattcata aaggtggtga gattgagctg cccaacctga accggagctg
    35041 cctggccttg ttgggttacg ctgaccacgc catcacgacc gatggtgata cttaacgcat
    35101 tcgccggaat ggtgatcgct ggctgcacct gaaaaccacc agccgtcacc agctgcccgt
    35161 tctgatccac ctggaaagag ccgtcacgga tataggctga tgagccatct ggcaacatta
    35221 cctggaaaaa gccctgccct ttaatcgcga catctttgct gttattggtc tgcgacagat
    35281 tgccctggct gtgtaagcgt tcagtggcga ccgggcgtac ccccgtgccg atttgtaata
    35341 cggagggtaa ggtggtttgt tcggaagact gtgcccccgg ctggcgaatg gtttgataaa
    35401 gcagatcttc aaacaccgcg cgctgacgct taaaaccgtt agtactgacg tttgccaggt
    35461 tgttggcaat gacgtccata ttggtttgct gggcgtcaag gcccgttttg gcgatccata
    35521 atgaactgat catataatgt cctgtagtgg attgctgaca agcagagcag aagcacgcga
    35581 cagtgtgaat ttcccctcac cctaaccctc tccccaaagg ggcgagggaa tagtccgcgc
    35641 gcttttattg caatgtcatc aatcgttcaa ttaactcatc gacagcagtt ggttggcacg
    35701 gcctgcgtta tcatcgacgc tgctgatcac cttcatctgc atttcaaaac gccgcgcgct
    35761 ggcaatcatg tcgctcattg ccgcaacggc attgacgtta ctgccttcca gaacccccga
    35821 catcacacgc aaggttggat ctgcctgcag taccggccct cgcgtggcct gggtttctgc
    35881 gcttaaacga aaaatgccgt cgtcaccgcg ctgcacttcg ctgcccgtgg ctttcaccag
    35941 tttcagacgc cctactggcg caactgtatt tgccggatcg cccggattga gcgccgagat
    36001 tgtgccatcg gcagcaatcg tgatttccgc cccttccggc acagcaattg gccccgcctc
    36061 gcctatcacc ggatgcccct gaattgtcag ttgcccggtg ggatcaacct gaatgctgcc
    36121 attacgcgta tacccttcgc tgccgtcagc ggtctgcacg gccagccatc ctgctgcaac
    36181 gcaacgtcca gcgggcgaga ggtgtaatcc atcttgccgg gcgtcatgtc tgcacccggt
    36241 gttgacgccg ttaccagcgt gcgcgtgggc agtgaaagcc cttccaccgg cactgcgcgc
    36301 aaggcgttta actgcgcgcg aaaaccgggc gtagaggcat tggccagatt actggcggtt
    36361 accgcctgtt gattcagcgt ctggctggct gctcccatcg cagtataaat tgcgtgatcc
    36421 attgagctat cccgtcagcg attagcgtaa gttaacccgc gtgttgagga tctggtcctg
    36481 ggttttgatg gtctgggcgt tagacttata gttacgctgg gcaacgatca tattgaccag
    36541 ttctttactg agatcgacgt tggacgcttc cagcgcaccg ttggtcagag tgccaaagtt
    36601 tcccgtcccg gctgtcccca acagcgccac gccagaagat tgcgtcgcag accagacgtt
    36661 gtcgccttcg gatgccagac cttcgttgtt ggcaaagttc gccagtacaa tctgccccag
    36721 cagttgggtt tgttcgttgg aattgttgcc gacaaccgta ccgtcatcat tgatttgata
    36781 actcaccaga tcgcccggtt tgtagccatt ctgggtggtt gccacaatgt tgttagcgcc
    36841 ggttgcgata ttattcgcca tcgcaccatc cactaatgtg ccattagcat taaattccag
    36901 tgttgtcgct gtcttcgcaa tgctgtttgg atcactgcta tcctgggtgt agacctgcca
    36961 gttattatcc ccggtcttca caaaatagac gctcatgtca tgagcattac cctgactgtc
    37021 gaaaacagtc accgaacctt ttttgttata gctatccgca ttgctggcgc tgaatggcgt
    37081 aacagtagga agcggatcac tggaattcag gttgatctgc atcgacgccg tggtggtagt
    37141 tttcgctgcc atcagggtat tcgggatcga aatattggtc ggattcgccc cttgctgaat
    37201 agtcggcggc gtaccggtta ccgggtaacc cgtcagctgt aaaccttgcg tattcaacag
    37261 gttacggttt tcatccagct taaattgtcc gttacggctg tagaacaccg aaccgttgct
    37321 gtctaccaga cggaaaaaac cgttctggct gatagcaacg tccagacctc gcccggtgtt
    37381 ggtggtcgtg ccatcggtaa agtcctgagt gataccggca acttttaccc ccagtcccac
    37441 tttcgaaccg gcaaacatat cggcaaaaga ggccgtgcct gatttaaagc cgtaggtggc
    37501 ggagttggcg atattgttgc caataacatc gaggttggtg gcggcagcgt ttaatccgct
    37561 aaccgcttga gaaaaggcca tgactgactc ctgaaatgtg aaggcttaaa ttatctgccg
    37621 tacttcgtcg agggtggtgg tgccgtaagt gccgagatcc agcgtattac cgctgttgcc
    37681 gcggatcaca ccctgcacca gagcaaactg cagcggctgg gcaaccagtt gtgtaccacc
    37741 gttactggcg ctaatcgcta cattgtaaga accgttcggc gcagtgctgc catcagtcaa
    37801 cgaaccgtcc caggtgaaac tgtgaactcc ggcggtcagt tcaccaatat caatggtgcg
    37861 cacaaccgcg ccatttttat cggtgatggt ggccgtaact ttgtctgccg cctgttgcag
    37921 ctcaacacca aacggcgtgg tcgtggtcac agccccttct tcactgccgg ttcccgcaag
    37981 aatagttgtg ccggggatca tcacgccgtg accgatcagg ttactggcct gtaacgactg
    38041 gctgttatca atctgtccgg aaatagatcc gagcgtggta ttgagttttt caatcccgct
    38101 gaccgtgctg atttgtgcca attgcgacgt cagctcgttg ttttccattg gattggtcgg
    38161 gtcctggttt ttcagctgcg ccaccagcaa agtcagaaaa ctgctttgta aatctgcggc
    38221 gttgctgccc gtgagcgaac tactgctggt ggtactgacg ccggtatttg tcggatcggt
    38281 ggtggttacc gcaatggaca tagctttctc ctttattgac cgagcgtaag ggttttcagc
    38341 atcatgcttt tcaccgtgtt gagcacttca acattggcct gatagctgcg tgacgccgac
    38401 atggtgttaa ccatctcccc gacgacatca acgttcggca tttttatgta gccctttgca
    38461 tctgccagcg gattacctgg ttcataaacc agtttgtccg gggcctgact ttcttaacat
    38521 cggcaacctt tacgccgcct gtcgcagcac ctggtgcagc gttaacctgg aataccacct
    38581 gttttgcccg atatggctgt ccatcaggac cggtcacgct atcagcattt gccagattac
    38641 tggccgccac gttcaggcgc tgggactggg cagttaacgc cgacccggcg atatcaaaaa
    38701 tattcagcag tgccatccgt taatttccgc tctgtaaaac gttcatcatg cctttgattt
    38761 gcccgctcaa cgcgctaagg ctcatctggt attgcaggct gttatcggca aactgggtgc
    38821 gttcgcgatc catatcgacg gtattaccgt caagcgaagg ctggtccgga atacggtatt
    38881 gcagttctgc ggtaggaggc gtcagcgcct gcgccgggat gtgctgcgtt gaggtcatcg
    38941 tcagtgcaac cacactggtt gcatcccgtc cacgttgcat gactttttta agttcactgg
    39001 caaaatcgat atcgcgcgcc tgataaccag gggtatcggc attggcgatg tttgctgcca
    39061 gcacttcctg acgctgggcg cgcagattga gcgcctcttg ttgaaaacgt aaggcggcgt
    39121 cgagcttatc gagcatatct cctccgcagg tatcaaaatt ctgccatcag cttaaatgcc
    39181 tttacgcgcg cgttatcggc ggaataaacg caaaatgggt cgctatttat gccgttgatg
    39241 gtcattgcgg acaggtacaa ttcacgttgt agaaatggct gggggcgaaa atgctgacaa
    39301 taaaacgtag cgtggcgatc atcgcgatac tgttcagtcc gttaagtacg gcgagcaatc
    39361 tcacatcgca attgcacaac ttttttagcg cccaactcgc gggggtaagt gatgaggttc
    39421 gtgtttctat tcgtacagcg cccaatctac taccgccatg cgagcagcca ttgctttcga
    39481 tgagcaataa ttcccgcctg tggggcaatg tgaatgtgtt ggcacgctgc ggtaacgaca
    39541 aacgatattt acaggttaat gtgcaggcca caggaaatta tgtggttgcc gcgatgccta
    39601 ttgtgcgggg aggaaagctg gaagctggca atgtcaaact gaaacgtgga cgcctggata
    39661 ccctgccacc gcgtacggtg ctggatatca atcaacttgt tgatgccgtt agcctgcgcg
    39721 atctatcacc cgatcaacct atccagttaa cccagtttcg ccaggcatgg cgggtaaaag
    39781 cgggacaacg cgtcaatgtg atcgccagcg gtgatgggtt tagcgccaac gcagaaggtc
    39841 aggcgctgaa caatgcagcc gtcgcacaga atgcgcgggt gcgcatggta tcggaacagg
    39901 tagtcagcgg cgttgttgat gcagatggga atattcttat aaacctgtaa gctgttaaag
    39961 attacccgtc ccttgccgat aaataagcaa cacatgataa aagcgccctc aatgaggaat
    40021 aaaccatgag tattgatcgc acttcgcctc tgaagcctgt aagcaccgtt caaccgcgcg
    40081 aaaccaccga cgcgccggta acgaacagcc gggcggcaaa aacaaccgcc tccaccagca
    40141 ccagtgtgac gttaagcgac gcgcaagcaa aactgatgca acccggcagc agtgatatca
    40201 atcttgaacg tgtcgaagcg ttaaaactgg cgattcgtaa cggtgaacta aaaatggaca
    40261 ccggcaaaat tgccgatgcg ctgatcaacg aagcgcagca agacttgcag agtaactgat
    40321 cgtatgacac gtcttgcaga gatcctcgac cagatgtccg ctgtgcttaa cgatctcaaa
    40381 acggtaatgg atcaagagca gcaacatctc tctatggggc agatcaacgg cagccagttg
    40441 caatggatta cagaacaaaa aagctcactg ctggcgacgc tggattacct cgaacagtta
    40501 cgcaagaaag aacccaatac agcaaatagc gttgatatta gtcaacgctg gcaggaaatt
    40561 actgtgaaaa cgcagcaact acgccaaatg aatcaacata acggctggtt actggaagga
    40621 cagattgagc gcaatcaaca ggcgctggaa atgttgaaac cgcatcagga accgacgcta
    40681 tatggggcga acggtcagac ctcaacaccc catcgcggca gtaaaaagat ttcgatctga
    40741 atattatctc cggcctgcac tgcaggccgg aatgcattgt tacaccgtcc ggcgggcaaa
    40801 ttctttaact ttgaagccca gtaccgccag tgcagcgaag tacgcggcaa tccccgccag
    40861 cacgaccgcc attaaacgca gtaagcgcca gggcatggta cccaatgacc actccggcat
    40921 gatatgtaac atacctaaaa gcacgccaga catcaccagt accgccacca ccagacgcaa
    40981 cagaaacgcc atccagccgg gttgcggagt aaagattttc tgcttacgca actgccagta
    41041 aagcagcgaa gcattcagac acgccgccag accaatagaa agtgacagcc cggcatgttt
    41101 caacggacca ataaacgcca ggttcatcaa ttgcgttaaa atcagcgtaa cgatggcaat
    41161 tttcactggc gttttaatgt cctggcggga ataaaagcca ggagccaaca ctttcactac
    41221 aatcaggcca atcaaaccca ccgagtaggc aattaacgcc cgctgggtca tcagcgcatc
    41281 aaacgcggta aatttaccgt actggaacag cgaaacggtt aacggacctg aaagaatgcc
    41341 caacgcaacc gcactcggca gtgccaacag gaaacaaaga cgcaaccccc agtccatcaa
    41401 acggttgtat tcatcatgat tgccactggc aaaacttttc gacagcgacg gcagcaaaat
    41461 ggtgccaagc gccacgccca gcacaccgga gggaaactcc attaagcggt cggcgtaata
    41521 catccaagac accgaaccgg aagcaagaaa cgaggcaaaa atggtgttga tgattaagga
    41581 gatctggctc acagagacgc caaggatcgc cggtcccatc tgtttcacca cgcgcattgc
    41641 tccagcatcg tggaagttaa tgcgcggcag gaccagcatg ccgatcttct tcaggtgcgg
    41701 tagctgatac accagctgca gaatgccgcc gaccgtaaca gcccacgcca gcgccagcac
    41761 cggtgggtta aagtacggtg cggcaaacag cgcgaaacca atcatgctga tgttaagcag
    41821 tgttggcgca aacgccggaa tcgagaagcg gttccacgta ttcagaatcg ctcccaccag
    41881 cgacgccagg gagatcagca agatataggg aaaggtaatc tttagtagct ggctggtcag
    41941 ggcaaatttg tcagctgtgt cagcgaagcc tggcgcggtc accatgatca cccacggtgc
    42001 ggcgagcatg ccagcgaccg tcacaaccgc cagcgcaagt gtcagcaggc cagaaacata
    42061 agagacaaag acccgcgtgg cgtcttcacc ctgcttactt ttatattccg ccagaatcgg
    42121 tacaaatgcc tgggaaaatg ccccttcggc aaagatacgg cgtaacaagt taggaagttt
    42181 aaaagcgacg aaaaaggcgt cggttgccat ccctgcgcca aagattctgg cgacaattgc
    42241 gtctcgtgcg aagccaagca cacgcgaaaa catggtcatc gagctgacgg cggccagcga
    42301 ttttaataaa ttcatcggtg ttctaatccg gacccacggg caaaacgcct gcaaggcagg
    42361 cgctgaaaga ggcgaatagt ctacctagat tatggtgaat tgctaccgcc agatgttaca
    42421 gggtttattc actcatcgca tcgcgccaga tcttgtcaac gatacgttgc gccagcactg
    42481 cctattcgcc ggcggtttgc ggaactgtct gattttacac gcattcaatg aagtgccgcg
    42541 cacagccgac aaatccacgt tgctcaagag tgctctgcca accaggaatc ggtttattca
    42601 ctacaccctg cccgcgctcc tcacgccatt cgcgcatatc cgtaatgtcg atgagcgcac
    42661 cgtcagtcac ggcctgcacg gtttcacgct gacttccggc acggcgatgc atacaagtgg
    42721 tgatctgcaa aggaccggcc gaaaaatggt gctcggcaaa cagcatttcg ccagcgtcgt
    42781 tagtcagtag cgtaccgcca tccagagagg ctttgccgcc cgacaaccac agcgcggtat
    42841 ccaccacatg cagataatca tccagcaacg tgaaataaag atcgtgtggc ccgacgctat
    42901 tgctacgatg tttatccatt cttagcgagg ctgcggtggc gagttgcgtt tttaactcac
    42961 cgtagagtgg tgcgaaacga cggttaaaac cgaccatcaa cgtcagtttt ttccgcgccg
    43021 ccagttccac cagcctttca gcatcgcgca gattttctgc cagcggttta tcgacacaga
    43081 catgtacccc cgcattgagt aacgtactga ccacgtcaaa gtggctggcg gtgctggagt
    43141 gcacaaaaac cgcatcacaa ctggcggcaa ggctggataa cgaatcggca taaggaatgc
    43201 gccagctgtc acaaattggc agggctttgg cgcgcgtagg cgaccaggct ccttgtaacg
    43261 tccagtcaga cgctgccgcc agcaccggta accacgcttt ttgcgcaatg ccgcctaatc
    43321 ccactacgcc gatacgtaat tttttcactt taatctccca gatgggccag caacgaatca
    43381 agacgttgtt tcagttctgc cacttcgatt tccagggctt cgacgcgggt ctgtaaatca
    43441 ccgtcaacca tgttcgacat agccgttacc gccggctgat cttcaacctc accactgaaa
    43501 agatgcatgt agcggctttc gcgtttaccc ggttcgcggg ccagacgcac cacaaaagga
    43561 ccatcttcgc gattcgccag ttgttccagc gtcgactcca cttccgccat atcgctgaat
    43621 tcatacattc gcgcggcgcg gctgcgcagt tcacccggcg tctgggcacc acgcaataac
    43681 aacgtggtga ttaacgccac ttctgcggcg ctcagtttca gatcgccaaa ttctgaatta
    43741 caaaaacgtt gctcatattt ggtgacccga ttaccaaaac cgctcactgt gcgtagataa
    43801 tgacgtttga ccagattatc cagctgttcc tgcacttcgg attcactcag attcatgacc
    43861 ggttcacggt tcgttttctg attacaggcc gtgactacac cattgactga gagcgggtat
    43921 tgctccggcg tggtcacctg cttttccagc aagcaaccaa ttacgcgagc ttccagggca
    43981 gtaagttgat atttcatgag attctcctta gcggccgggc gtccagtctg gggtagttaa
    44041 tgccgtcagt acgtgatcgc gccattgtcc atcaatcaac agatagtctt tcgcatagcc
    44101 ttctttttca aaacccagtc gcgccagtaa atcaccgctg cgtttattgt gcggcatata
    44161 attagccata atgcgatgaa tatgttgggt gcgctgcata taacgaatgg ctgcggtcag
    44221 ggcttcaaac atgagtcctt tgccctgcca tttttgccca atcgaataac cgagatagca
    44281 ggcatgaaaa gagccgcgaa caacattgga aaaattggca acgccaataa tctctttttc
    44341 gtccgggtcg aataagccaa agtagaaagc tgaaccttgt ttatgaaatt cgttaatcat
    44401 ccctagcctg gcctgccagc ctgatggata acagtggctt tcgtcgcgca ctggctccca
    44461 gggcttgagg aaatggcgat tctctgcgta ataatccgcc agacgccagg catcacgatc
    44521 atgcaccaga cgcacgacca gtcggtctgt ggttaagcgc acttttggca cgttactgcg
    44581 atagccaaac attctatacc tactccttcc cgtaacgtct ttaatacgct gtaagtatta
    44641 ctatacctgc gcaaccagac cctgtgaaaa gtccaaaatg ccattttctg cattattcac
    44701 atatttgata aaaactctct atcaaagcga cgttttgata aaaaaatact gtccccgaag
    44761 tgatgggaaa agtcttagcg gcaatgcaga atagaaagtg ctcatctttt atttcccggg
    44821 aggggaaatg tcccgcgtat cgcaggcgag gaacctgggt aaatatttcc tgctcatcga
    44881 taatatgctg gtcgtgctgg ggttctttgt tgtcttcccg ctgatctcta tccgcttcgt
    44941 tgatcaaatg ggctgggccg ccgtcatggt cggtattgct ctcggtctac gccagtttat
    45001 tcagcaaggt ctgggtattt tcggcggcgc aattgccgac cgctttggtg ccaaaccgat
    45061 gattgttacc ggtatgctga tgcgcgccgc cggattcgcc acaatgggta tcgcccacga
    45121 accgtggcta ttgtggtttt catgcctgct ctcgggactc ggcggcacgt tgtttgatcc
    45181 gccgcgttcg gcgctggtgg tgaaactaat ccgtccacaa caacgtggtc gttttttctc
    45241 gctgttgatg atgcaggaca gtgccagtgc ggtcattggc gcattgttgg ggagctggct
    45301 gttgcaatac gactttcgcc tggtctgcgc cacaggggcc gttttgtttg tgctatgtgc
    45361 ggcgttcaat gcgtggttgt taccggcatg gaaactctcc accgtacgca cgcccgttcg
    45421 cgaaggcatg acccgcgtga tgcgtgacaa gcgttttgtc acctatgtcc tgacgctggc
    45481 gggttactac atgctggctg tacaagtgat gctgatgctg ccaattatgg tcaacgacgt
    45541 ggctggcgcg ccctctgccg ttaaatggat gtatgccatt gaagcgtgtc tgtcgttaac
    45601 gttgctctac cctatcgccc gctggagtga aaagcatttt cgtctggaac accggttgat
    45661 ggctgggctg ttgataatgt cattaagcat gatgccggtg ggcatggtca gcggcctaca
    45721 acaacttttc accctgattt gtctgtttta tatcgggtcg atcattgccg agcctgcgcg
    45781 tgaaacctta agtgcttcgc tggcggacgc aagagctcgc ggcagctata tggggtttag
    45841 ccgtctgggt ctggcgattg gcggcgctat tggttatatc ggtggtggct ggctgtttga
    45901 cctgggcaaa tcggcgcacc agccagagct tccgtggatg atgctgggaa ttattggcat
    45961 cttcactttc cttgcgctgg gttggcagtt tagtcagaaa cgcgccgcgc gtcgtttgct
    46021 ggaacgcgac gcctgatttg atactccccc ctgactcctc catactgaaa gtgaactgac
    46081 agccaaatgg aggagtcacg tgaagctata catttacgat cactgccctt actgcctcaa
    46141 agcccgcatg attttcggcc tgaaaaatat ccccgtcgaa ttacatgttc tgctcaacga
    46201 cgacgcagaa acacccaccc ggatggtcgg tcaaaaacag gttcccattc tgcaaaaaga
    46261 tgacagccgc tatatgccag aaagcatgga tatcgttcac tatgtcgata aactcgacgg
    46321 caaaccgtta ctgaccggca aacgttcccc tgccattgaa gagtggctgc gcaaggtcaa
    46381 tggctacgtc aacaaactgc tgttgccgcg ttttgccaaa tcggcatttg atgagttttc
    46441 tactcccgcc gcgcgcaaat atttcgtcga caagaaagag gccagcgcgg gtaattttgc
    46501 cgacctgctg gcccactctg acggtctgat taagaatatc agcgatgatt tgcgtgcgct
    46561 ggacaaactg atcgtcaaac cgaacgccgt gaatggcgaa ctttcggaag atgatattca
    46621 gctatttccg ctactgcgta atctgacgct ggtagccgga atcaactggc caagccgcgt
    46681 tgctaattac cgcgataata tggcgaaaca gacacaaatc aatttgttat catcaatggc
    46741 gatttaatcc tgacccgccg cggcagcctg tattcgcggg ttgccgccgt tttcagtttt
    46801 ttctgctggc gagtttatcg ctggtgacac atgctgcgtt gtgtcaggat attgacgcag
    46861 atttcatgtc gaggactacc atgaacaagt ttttatttgc cgctgcattg atcgtcagtg
    46921 gcctgctcgt tggctgtaat caactcaccc aatacaccat caccgaacaa gaaattaacc
    46981 agtcgcttgc gaaacataat aatttctcaa aagatatcgg tttacccggc gtggctgacg
    47041 cccatattgt tctgacaaac ttgaccagcc aaattggtcg cgaagagccg aataaggtta
    47101 ccctaaccgg agacgccaat ctggacatga actccctgtt cggtagtcag aaagcgacca
    47161 tgaaactgaa gctgaaagcg ctgcccgtgt ttgataaaga aaaaggtgcg atcttcctga
    47221 aagagatgga agtggtcgat gcgacggtac aaccggaaaa aatgcaaacg gtgatgcaaa
    47281 cgttgcttcc ctatttgaac caggcattac gcaattactt taaccagcaa cctgcttacg
    47341 tcctgcgcga agatggcagt cagggcgaag caatggcgaa aaaactggcg aaaggcattg
    47401 aagtgaagcc aggcgaaatt gtcattccgt ttacggatta atgacgaggg cgcattcgcg
    47461 ccctttattt ttcgtgcaaa ggaaaacgtt tccgcttatc ctttgtgtcc ggcaaaaaca
    47521 tcccttcagc cggagcatag agattaatga ctgcaccatc ccaggtatta aagatccgcc
    47581 gcccagacga ctggcacctt cacctccgcg atggcgacat gttaaaaact gtcgtgccgt
    47641 ataccagcga aatttatgga cgggctatcg taatgcccaa tctggctccg cccgtgacca
    47701 cagttgaggc tgccgtggcg tatcgccaac gtattcttga cgccgtacct gccgggcacg
    47761 atttcacccc attgatgacc tgttatttaa cagattcgct ggatcctaat gagctggagc
    47821 gcggatttaa tgaagccgtg ttcaccgctg caaaacttta cccggcaaac gcaaccacta
    47881 actccagcca cggcgtgacg tcaattgacg caatcatgcc ggtacttgag cgcatggaaa
    47941 aaatcggtat gccgctactg gtgcatggtg aagtgacaca tgcagatatc gacatttttg
    48001 atcgtgaagc gcgctttata gaaagcgtga tggaacctct gcgccagcgc ctgactgcgc
    48061 tgaaagtcgt ttttgagcac atcaccacca aagatgctgc cgactatgtc cgtgacggaa
    48121 atgaacggct ggctgccacc atcactccgc agcatctgat gtttaaccgc aaccatatgc
    48181 tggttggtgg cgtgcgtccg cacctgtatt gtttacccat cctcaaacgc aatattcacc
    48241 aacaggcatt gcgtgaactg gtcgccagcg gttttaatcg agtattcctc ggtacggatt
    48301 ctgcgccaca tgcacgtcat cgcaaagaga gcagttgcgg ctgcgcgggc tgcttcaacg
    48361 ccccaaccgc gctgggcagt tacgctaccg tctttgaaga gatgaatgct ctgcagcact
    48421 ttgaagcatt ctgttctgta aacggcccgc agttctatgg cttgccggtc aacgacacat
    48481 tcatcgaact ggtacgtgaa gagcaacagg ttgctgaaag catcgcactg actgatgaca
    48541 ccctggtgcc attcctcgcc ggggaaacgg tacgctggtc cgttaaacaa taaaaatcgt
    48601 agcttcctgt tgtcattagg ttattttacc tgtataaata accagtatat tcaacagggg
    48661 gctattatgc gaattgaagt caccatagcg aaaacttctc cattgccagc tggggctatt
    48721 gacgccctgg ctggcgaact ttcccgccgt attcagtatg cgtttcctga taatgaaggc
    48781 cacgtatcgg tacgttatgc cgcagcgaat aatttatcgg ttattggcgc aacaaaagaa
    48841 gataaacagc gcattagcga aattctccag gaaacgtggg aaagcgccga tgactggttt
    48901 gtcagcgaat aatatgcagt gatttttgtt tttgccggat cgccccggct tttttatttt
    48961 acttacgaat tttgagtaca aaatttctac acttcctcaa aataattgca caatatgcta
    49021 aaaaaatatg aatgacatgc tgttttattg ttcatataaa tcttaaaagc agaaatttat
    49081 ctgttccccc ctttaaaaat tcggtgaata cccttactta ttggtatact gaagttgctt
    49141 cagataaaaa cgcattgaac ctcgaataac gttgtctagt aacacgaatt agggggccat
    49201 gatggaaaaa aataatgaag tcattcagac tcatccgctc gtagggtggg acatcagcac
    49261 cgttgatagc tatgatgcgc tgatgttgcg tttgcactac cagaccccaa ataagtccga
    49321 gcaggaaggg actgaagttg gtcagacgct ctggttaacc actgatgttg ccagacaatt
    49381 tatttcgata ttagaagcag gaatcgccaa aattgaatcc ggtgatttcc cggtaaacga
    49441 gtatcggcgt cattaacatg acatgaccat ccacccaaaa ggcacctcac cggtgccttt
    49501 accatttctg ggttgtatat ctgcattcag ttaattactc tgcttaaacc atgagagcag
    49561 agagaaaaaa atgaaatacg atctcatcat tattggcagc ggttccgtag gcgctgccgc
    49621 cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg accgacgccc atatgccacc
    49681 gcatcaacac ggcagccacc acggcgatac gcgattaatt cgccatgctt atggtgaagg
    49741 cgaaaagtat gtcccgttgg tcctccgcgc gcaaacgctg tgggatgaac tttcccgcca
    49801 caacgaagat gatcccattt ttgtacgctc tggtgtcatt aacctcggcc cggctgactc
    49861 cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg caactcaacg ttgaaaagct
    49921 cgatgcgcaa gggattatgg cccgctggcc agaaatacgc gtcccggaca actacatcgg
    49981 cttatttgag actgattccg gttttttgcg cagcgaactg gcgattaaaa cctggatcca
    50041 actggcgaag gaagcgggct gtgcgcaact gttcaactgc ccggtcaccg caattcgtca
    50101 tgacgatgat ggcgtaacta ttgaaacggt tgacggtgag tatcaggcga aaaaagcgat
    50161 tgtctgcgcg ggaacatggg taaaagacct gctcccggag ctgcctgtcc agcccgtacg
    50221 taaagtattt gcctggtatc aggccgatgg ccgctatagc gtgaagaata aattcccggc
    50281 gtttaccggt gaactgccca atggcgatca atattatggt tttccggcag aaaacgacgc
    50341 gttgaagatt ggcaaacata acggaggcca ggttatccat tcagcggatg aacgtgttcc
    50401 gtttgcggaa gtggccagtg atgggtcaga agccttcccg ttcttgcgca atgtattgcc
    50461 gggtatcggt tgctgcctgt acggcgctgc ctgcacctat gataattcgc ctgacgaaga
    50521 ttttattatc gataccctac ccggccacga taatacactg ctcattaccg gcctgagtgg
    50581 gcacggtttt aaatttgcgt cagttttagg ggaaatagct gccgattttg cgcaagacca
    50641 aaaaagcgat tttgatttga cgccattcag gctttcccgc ttccaataat catcattacg
    50701 gcctccagaa tgaggccgtt tttacaggag tatttaatgc gtcggctgtt gcactatctc
    50761 atcaataata ttcgcgagca tctgatgctt tatctttttc tctggggatt gttggcaatt
    50821 atggatctga tctatgtgtt ttatttttag ttttccgcac tgacataaac tgacaaatat
    50881 ttaatctccc tttcatttag cgtgaaaaat tgaatgcgat tattcctgct tctacagcac
    50941 tcaccagaca tctctgatgc tgaacccact aaacttatga agattctttc attattaatt
    51001 caaaattatt gaaataaaaa taaatatcat tcaatttgtt atttttgtgt tgcaattcat
    51061 tctccgacac gccattttct ttctctcgca aattcgtatt ggatgcactt atgtcattca
    51121 caaatacccc tgaacgctat ggtgttatat ctgcggcctt ccattggcta agcgcaatta
    51181 ttgtctacgg catgtttgct ttaggtctgt ggatggtcac gctcagttat tacgatggct
    51241 ggtatcacaa agcacccgaa ctgcataaaa gcatcggtat tttgttaatg atggggctgg
    51301 ttattcgcgt tctgtggcgt gtcatatctc caccgccggg tccgctgccg agttattcgc
    51361 ccatgactcg tcttgccgca aaagctggac atctcgcatt gtgcctattg ctgttcgcca
    51421 tcggcatcag cggctatctg atctcaactg ccgatggtaa accgatcagc gtttttggct
    51481 ggtttgacgt ccccgcgacc cttgccgacg ctggcgcaca ggcagacttt gccggtgccc
    51541 tgcatttttg gctcgcgtgg agcgtcgtag tactgtctgt tatgcacgga tttatggccc
    51601 tgaagcatca tttcatcgat aaagacgaca ctctgaagcg catgctggga aagtcgtcat
    51661 ctgactatgg agtataaaaa atgaaaaaaa gcctgcttgg tttaaccttc gcgtccctga
    51721 tgttctctgc cggttcagcg gttgccgccg attacaaaat tgacaaagaa ggtcagcacg
    51781 cctttgttaa cttccgcata cagcatcttg gctatagctg gttatatggc acctttaaag
    51841 atttcgacgg tacttttacc tttgatgaaa aaaatccggc tgccgataaa gtgaatgtga
    51901 caattaacac caccagcgtc gatactaacc acgccgaacg cgataaacat cttcgcagtg
    51961 cggattttct caataccaca aaatatccac aggcaacatt cacctccacc agcgtgaaga
    52021 aagacggtga cgaactggat attaccggcg atctgacgct gaatggcgta accaaacccg
    52081 tcacgctgga agcgaaatta attggtcagg gcgacgaccc atggggcggt aaacgtgcag
    52141 gctttgaggc cgaaggcaaa attaagctca aagacttcaa tatcaagaca gatttaggtc
    52201 cagcttctca ggaagtggat ctgattattt cagtggaagg cgtacagcag aagtaagagt
    52261 gaaatgttgc cggatggcac gcatccggca atgatattta ttctgttgga tcaggaatac
    52321 ccagtgttgt attcagacgt ccacgagact tattaaagat cttattgcca ttttcacgtc
    52381 ctgcccgacg gcgtcgctgt tcttctggcg gtaacgcgct ttcttcgcag caaatctcac
    52441 tacaacaacc tttgtatttt tccgcgcata ctggacactg aataaacagc aggtggcagc
    52501 catcattttt acagttggta tggctgtcgc acggcgcgcc gcactggtgg caatgcgcga
    52561 taatctcatc cgatatacgt tcgcccatcc gctcgtcaaa aacaaaattt ttgccaataa
    52621 aacgcaccgg caagccctgc tcgcgcgcct tacgggcgta ttcaataatt ccgccctcga
    52681 tatgccagac tttgttgaat ccgttatgtt tcatccaggc actggccttt tcacaacgaa
    52741 tgccgccggt gcagtacatg acgatttttt tatctttatg tgcctgcatc atctcgactg
    52801 cttttggcag ctgctcacgg aaggtatctg ccggaatttc gagcgcgttt tcaaagtgcc
    52861 ccacttcata ctcatagtgg ttacgcatgt cgataaatag tgcatcggga tcgtcaagca
    52921 tggcgttcac ttccgccgct tgcagatact caccaacatt gctggcatca aagtgaggat
    52981 cgtcaatacc gtcggcaaca atgcgatcgc gtaccttcat gcgcagtacc cagaaggatt
    53041 tcccgtcatc atccaacgcg atattcaggc gtaaaccctc taaagccggg tcgaaggcat
    53101 agagctgcgc gcgaaatgtt tcaacattgc tcgcaggtac gctgatttgc gcgttaatgc
    53161 cctcatgcgc cagatacact cgcccaaaaa cattcagcgc ggtaaacagc tgatataaag
    53221 cgtcacgggt cgccttagga tcggcgatgt ggaaatactt gtaaaacgaa atggtggttc
    53281 gcggttcgct ctcagccaac attttggctt ttaacgcgtc gttggaaatg cggttgtgta
    53341 acactggcat ggtgtacggt tcctgcgaga tgggaaagta aaaatccgcg gcatgatata
    53401 gcaattatcg ataattaaca tccacacatt ttacgctaca tttgcgcatt aaaaattatt
    53461 tgttatttac aagcgcggca atttcgccca gtcttcagcc acaaattttg gttgcgggcg
    53521 aaaaaatgcg acaatacata caattgcccg tataggttga aaaacaggat tgatatgacg
    53581 aatctaccca agttctccac cgcactgctt catccgcgtt attggttaac ctggttgggt
    53641 attggcgtac tttggttggt cgtgcaattg ccctacccga ttatctaccg cctcggttgt
    53701 ggattaggaa aactggcgtt acgttttatg aaacgacgcg caaaaattgt gcatcgcaac
    53761 ctggaactgt gcttcccgga aatgagcgaa caagaacgcc gtaaaatggt ggtgaagaat
    53821 ttcgaatccg ttggcatggg cctgatggaa accggtatgg cgtggttctg gccggaccgc
    53881 cgaatcgccc gctggacgga agtgatcggc atggaacaca ttcgtgacgt gcaggcgcaa
    53941 aaacgcggca tcctgttagt tggcatccat tttctgacac tggagctggg cgcgcggcag
    54001 tttggtatgc aggaaccggg gattggcgtt taccgcccga acgataatcc actgattgac
    54061 tggctacaaa cctggggccg tttgcgctca aataaatcga tgctcgaccg caaagattta
    54121 aaaggcatga ttaaagccct gaaaaaaggc gaagtggtct ggtacgcacc agatcatgat
    54181 tacggcccgc gctcaagcgt tttcgtcccg ttatttgccg ttgagcaggc tgcgaccacg
    54241 accggaacct ggatgctggc acggatgtcc ggcgcatgtc tggtgccctt cgttccacgc
    54301 cgtaagccag atggcaaagg gtatcaattg attatgctgc caccagagtg ttctccgcca
    54361 ctggatgatg ccgaaactac cgccgcgtgg atgaacaaag tggtcgaaaa atgcatcatg
    54421 atggcaccag agcagtatat gtggttacac cgtcgcttta aaacacgccc ggaaggcgtt
    54481 ccttcacgct attaaatctc ccatgccgga tgcttcagaa tggcatccgg cattaccaca
    54541 gcaaatcccc ctgatttagc gataaaagct ctctggattg cgccccctgg aagtcgggcg
    54601 cataattagt gtgtttatct tttcttctta tgttcaccgc gcctggcgca ccaacagcgg
    54661 attgctatgt caccctgtga aaatgacacc cctataaact ggaaacgaaa cctgatcgtc
    54721 gcctggctag gctgttttct taccggggcc gccttcagtc tggtaatgcc cttcttaccc
    54781 ctctacgttg agcagcttgg tgttaccggc cactccgccc tgaatatgtg gtccggtatt
    54841 gtcttcagta taatgggttc ctaaacacat cattttaggt attgaatatg agacgggcaa
    54901 tcagctacat cagattttca agtgaacgac aactgaaagg ggactctgta cgccgacaat
    54961 ctaagctggt tactgactgg cttgataaga accctgagtt ttacttggat tcatccttga
    55021 gctttaaaga tttggggaaa agtgctttta gtggtaagca cctgaaaggt ggtctaggtg
    55081 actttttaac cgctatcgag aaagggcttg ttaaagctgg tgatacgcta ctaatcgaga
    55141 gtttagaccg tctgtcacgt caggatattg atattgcttc tgagctatta cgccgtatct
    55201 taagggctgg tgtagatgta gtcacactga gcgatggaga acattacaca cgagaaagtt
    55261 taaaagaccc gttagccctg attaaaagca ttctgataat gcagcgggcg catgaggaaa
    55321 gtttgagaaa gtctgagcgt gtccaagctg catggaaccg taaaaaagaa ctcatctccg
    55381 aaggtatcaa ggtatctagg cgttgtcctg catggttaag gctgaatgat gaccgtcgaa
    55441 cattcacaat cattcctgat aaagtggaag ttgttaaacg agcatttgac ctgagattac
    55501 agggtttaag cttttgggct atcaccagaa ctttaaacga tgaaggtcac ttgtcattga
    55561 atcaatacac acccaaacaa aaggggtggt ctgatactgc tgtaaagaaa ctgctcagaa
    55621 atcgtgctgt gattggatgt ttcacacctg ctggacgtga agaagttcag ggttactacc
    55681 ccgcaataat cagtgagtca ctattctatc gagtacagca actgaacaca gggcaatatg
    55741 gtagggcttc tgtatcaagt aatccgctat cagtcaactt gttcaggggt atcatcaaat
//
LOCUS       DAZXUL010000007        55436 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZXUL010000007 DAZXUL010000000
VERSION     DAZXUL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55436
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.007"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            154..1640
                     /locus_tag="ACH3L1_000396"
                     /pseudo
     CDS             154..1640
                     /locus_tag="ACH3L1_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            1655..2389
                     /gene="ypdB"
                     /locus_tag="ACH3L1_000397"
     CDS             1655..2389
                     /gene="ypdB"
                     /locus_tag="ACH3L1_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFT4075306.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2402..3259
                     /locus_tag="ACH3L1_000398"
                     /pseudo
     CDS             2402..3259
                     /locus_tag="ACH3L1_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3262..5757)
                     /gene="ptsP"
                     /locus_tag="ACH3L1_000399"
     CDS             complement(3262..5757)
                     /gene="ptsP"
                     /locus_tag="ACH3L1_000399"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFT4075307.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5782..6819)
                     /gene="ypdE"
                     /locus_tag="ACH3L1_000400"
     CDS             complement(5782..6819)
                     /gene="ypdE"
                     /locus_tag="ACH3L1_000400"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFT4075308.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6819..7235)
                     /locus_tag="ACH3L1_000401"
     CDS             complement(6819..7235)
                     /locus_tag="ACH3L1_000401"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFT4075309.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(7232..7339)
                     /locus_tag="ACH3L1_000402"
     CDS             complement(7232..7339)
                     /locus_tag="ACH3L1_000402"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075310.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            complement(7300..7902)
                     /gene="ypdF"
                     /locus_tag="ACH3L1_000403"
                     /pseudo
     CDS             complement(7300..7902)
                     /gene="ypdF"
                     /locus_tag="ACH3L1_000403"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7917..9164)
                     /locus_tag="ACH3L1_000404"
     CDS             complement(7917..9164)
                     /locus_tag="ACH3L1_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFT4075311.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9186..9512)
                     /locus_tag="ACH3L1_000405"
     CDS             complement(9186..9512)
                     /locus_tag="ACH3L1_000405"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFT4075312.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9731..10696)
                     /gene="glk"
                     /locus_tag="ACH3L1_000406"
     CDS             complement(9731..10696)
                     /gene="glk"
                     /locus_tag="ACH3L1_000406"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFT4075313.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            10900..12156
                     /locus_tag="ACH3L1_000407"
     CDS             10900..12156
                     /locus_tag="ACH3L1_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFT4075314.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12271..12597
                     /gene="ypeC"
                     /locus_tag="ACH3L1_000408"
     CDS             12271..12597
                     /gene="ypeC"
                     /locus_tag="ACH3L1_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFT4075315.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12738..13976)
                     /locus_tag="ACH3L1_000409"
     CDS             complement(12738..13976)
                     /locus_tag="ACH3L1_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFT4075316.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14312..15514
                     /gene="nupC"
                     /locus_tag="ACH3L1_000410"
     CDS             14312..15514
                     /gene="nupC"
                     /locus_tag="ACH3L1_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFT4075317.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15564..17676)
                     /locus_tag="ACH3L1_000411"
                     /pseudo
     CDS             complement(15564..17676)
                     /locus_tag="ACH3L1_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(17884..17959)
                     /locus_tag="ACH3L1_000412"
     tRNA            complement(17884..17959)
                     /locus_tag="ACH3L1_000412"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17924..17926),aa:Ala,seq:ggc)
     gene            complement(17999..18074)
                     /locus_tag="ACH3L1_000413"
     tRNA            complement(17999..18074)
                     /locus_tag="ACH3L1_000413"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18039..18041),aa:Ala,seq:ggc)
     gene            18295..18654
                     /locus_tag="ACH3L1_000414"
     CDS             18295..18654
                     /locus_tag="ACH3L1_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFT4075318.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18656..19048
                     /locus_tag="ACH3L1_000415"
     CDS             18656..19048
                     /locus_tag="ACH3L1_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFT4075319.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19100..20515)
                     /gene="gltX"
                     /locus_tag="ACH3L1_000416"
     CDS             complement(19100..20515)
                     /gene="gltX"
                     /locus_tag="ACH3L1_000416"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFT4075320.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20774..20849
                     /locus_tag="ACH3L1_000417"
     tRNA            20774..20849
                     /locus_tag="ACH3L1_000417"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20807..20809,aa:Val,seq:tac)
     gene            20854..20929
                     /locus_tag="ACH3L1_000418"
     tRNA            20854..20929
                     /locus_tag="ACH3L1_000418"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20887..20889,aa:Lys,seq:ttt)
     gene            20960..21085
                     /locus_tag="ACH3L1_000419"
     ncRNA           20960..21085
                     /ncRNA_class="other"
                     /locus_tag="ACH3L1_000419"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21205..21546
                     /locus_tag="ACH3L1_000420"
     CDS             21205..21546
                     /locus_tag="ACH3L1_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFT4075321.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(21537..22463)
                     /locus_tag="ACH3L1_000421"
     CDS             complement(21537..22463)
                     /locus_tag="ACH3L1_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFT4075322.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            22553..23551
                     /locus_tag="ACH3L1_000422"
     CDS             22553..23551
                     /locus_tag="ACH3L1_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFT4075323.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23548..23766)
                     /locus_tag="ACH3L1_000423"
     CDS             complement(23548..23766)
                     /locus_tag="ACH3L1_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFT4075324.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23768..25783)
                     /gene="ligA"
                     /locus_tag="ACH3L1_000424"
     CDS             complement(23768..25783)
                     /gene="ligA"
                     /locus_tag="ACH3L1_000424"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFT4075325.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25854..26840)
                     /gene="zipA"
                     /locus_tag="ACH3L1_000425"
     CDS             complement(25854..26840)
                     /gene="zipA"
                     /locus_tag="ACH3L1_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFT4075326.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            27070..27831
                     /gene="cysZ"
                     /locus_tag="ACH3L1_000426"
     CDS             27070..27831
                     /gene="cysZ"
                     /locus_tag="ACH3L1_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFT4075327.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            28016..28987
                     /gene="cysK"
                     /locus_tag="ACH3L1_000427"
     CDS             28016..28987
                     /gene="cysK"
                     /locus_tag="ACH3L1_000427"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFT4075328.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKDIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29371..29628
                     /gene="ptsH"
                     /locus_tag="ACH3L1_000428"
     CDS             29371..29628
                     /gene="ptsH"
                     /locus_tag="ACH3L1_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFT4075329.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29673..31400
                     /gene="ptsI"
                     /locus_tag="ACH3L1_000429"
     CDS             29673..31400
                     /gene="ptsI"
                     /locus_tag="ACH3L1_000429"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFT4075330.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31441..31950
                     /gene="crr"
                     /locus_tag="ACH3L1_000430"
     CDS             31441..31950
                     /gene="crr"
                     /locus_tag="ACH3L1_000430"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFT4075331.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(31992..32843)
                     /gene="pdxK"
                     /locus_tag="ACH3L1_000431"
                     /gene_synonym="thiJ"
     CDS             complement(31992..32843)
                     /gene="pdxK"
                     /locus_tag="ACH3L1_000431"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFT4075332.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            32948..33322
                     /locus_tag="ACH3L1_000432"
     CDS             32948..33322
                     /locus_tag="ACH3L1_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFT4075333.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            33355..34078
                     /locus_tag="ACH3L1_000433"
                     /pseudo
     CDS             33355..34078
                     /locus_tag="ACH3L1_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(34282..35193)
                     /gene="cysM"
                     /locus_tag="ACH3L1_000434"
     CDS             complement(34282..35193)
                     /gene="cysM"
                     /locus_tag="ACH3L1_000434"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFT4075334.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(35327..36424)
                     /gene="cysA"
                     /locus_tag="ACH3L1_000435"
     CDS             complement(35327..36424)
                     /gene="cysA"
                     /locus_tag="ACH3L1_000435"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFT4075335.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(36414..37289)
                     /gene="cysW"
                     /locus_tag="ACH3L1_000436"
     CDS             complement(36414..37289)
                     /gene="cysW"
                     /locus_tag="ACH3L1_000436"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFT4075336.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(37289..38122)
                     /gene="cysT"
                     /locus_tag="ACH3L1_000437"
     CDS             complement(37289..38122)
                     /gene="cysT"
                     /locus_tag="ACH3L1_000437"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFT4075337.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(38122..39138)
                     /gene="cysP"
                     /locus_tag="ACH3L1_000438"
     CDS             complement(38122..39138)
                     /gene="cysP"
                     /locus_tag="ACH3L1_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFT4075338.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(39442..40233)
                     /gene="ucpA"
                     /locus_tag="ACH3L1_000439"
     CDS             complement(39442..40233)
                     /gene="ucpA"
                     /locus_tag="ACH3L1_000439"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFT4075339.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(40362..41220)
                     /gene="murR"
                     /locus_tag="ACH3L1_000440"
                     /pseudo
     CDS             complement(40362..41220)
                     /gene="murR"
                     /locus_tag="ACH3L1_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            41384..42280
                     /gene="murQ"
                     /locus_tag="ACH3L1_000441"
     CDS             41384..42280
                     /gene="murQ"
                     /locus_tag="ACH3L1_000441"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFT4075340.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            42284..43708
                     /gene="murP"
                     /locus_tag="ACH3L1_000442"
     CDS             42284..43708
                     /gene="murP"
                     /locus_tag="ACH3L1_000442"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFT4075341.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            43713..43799
                     /locus_tag="ACH3L1_000443"
                     /pseudo
     CDS             43713..43799
                     /locus_tag="ACH3L1_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(43888..44787)
                     /gene="yfeX"
                     /locus_tag="ACH3L1_000444"
     CDS             complement(43888..44787)
                     /gene="yfeX"
                     /locus_tag="ACH3L1_000444"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFT4075342.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(44883..45458)
                     /locus_tag="ACH3L1_000445"
     CDS             complement(44883..45458)
                     /locus_tag="ACH3L1_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFT4075343.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(45519..45968)
                     /locus_tag="ACH3L1_000446"
     CDS             complement(45519..45968)
                     /locus_tag="ACH3L1_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFT4075344.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(45955..46380)
                     /locus_tag="ACH3L1_000447"
     CDS             complement(45955..46380)
                     /locus_tag="ACH3L1_000447"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFT4075345.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            46594..47463
                     /gene="amiA"
                     /locus_tag="ACH3L1_000448"
     CDS             46594..47463
                     /gene="amiA"
                     /locus_tag="ACH3L1_000448"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFT4075346.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            47467..48366
                     /gene="hemF"
                     /locus_tag="ACH3L1_000449"
     CDS             47467..48366
                     /gene="hemF"
                     /locus_tag="ACH3L1_000449"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFT4075347.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(48372..49424)
                     /gene="eutR"
                     /locus_tag="ACH3L1_000450"
                     /pseudo
     CDS             complement(48372..49424)
                     /gene="eutR"
                     /locus_tag="ACH3L1_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(49470..49970)
                     /gene="eutK"
                     /locus_tag="ACH3L1_000451"
     CDS             complement(49470..49970)
                     /gene="eutK"
                     /locus_tag="ACH3L1_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFT4075348.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(49983..50642)
                     /gene="eutL"
                     /locus_tag="ACH3L1_000452"
     CDS             complement(49983..50642)
                     /gene="eutL"
                     /locus_tag="ACH3L1_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFT4075349.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(50652..50720)
                     /locus_tag="ACH3L1_000453"
                     /pseudo
     CDS             complement(50652..50720)
                     /locus_tag="ACH3L1_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            51824..52615
                     /locus_tag="ACH3L1_000454"
     CDS             51824..52615
                     /locus_tag="ACH3L1_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075350.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            53335..53730
                     /locus_tag="ACH3L1_000455"
     CDS             53335..53730
                     /locus_tag="ACH3L1_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFT4075351.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            53748..54011
                     /locus_tag="ACH3L1_000456"
     CDS             53748..54011
                     /locus_tag="ACH3L1_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075352.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            54034..54576
                     /locus_tag="ACH3L1_000457"
     CDS             54034..54576
                     /locus_tag="ACH3L1_000457"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075353.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            54715..55002
                     /locus_tag="ACH3L1_000458"
     CDS             54715..55002
                     /locus_tag="ACH3L1_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075354.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            55041..55247
                     /locus_tag="ACH3L1_000459"
     CDS             55041..55247
                     /locus_tag="ACH3L1_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075355.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
BASE COUNT        13839 a        14704 c        13700 g        13193 t
ORIGIN      
        1 agccagaagt gcattatcaa gagatacatt gctaatacac catttatctt ttaatcccct
       61 tttaaattta tggtttttcc tcaaaaacgg cctgctggtc atcagccttc aggttaccta
      121 tcatagaggt ttaatcctta ttcagagtca cccgtgcacg aaatattcaa catgctgctg
      181 gcggtcttcg atcgggcagc gttaatgctt atctgcctgt tctttctcat ccgtatccgc
      241 ctgtttcgcg aactgttgca caagtcggcg cactccccaa aagaattgct cgccgttacc
      301 gccattttct cgctgttcgc cctgttcagc acctggtccg gcgttcccgt agaaggctcg
      361 ctggtgaacg tacgtattat cgcggtgatg tccggcggga ttctgtttgg cccgtgggta
      421 ggcatcatta ccggcgtgat tgcgggtatt caccggtatt taattgatat cggcggcgtg
      481 acggcgatcc cctgctttat caccagcatt ctggcgggtt gtatatcagg ctggataaac
      541 ctgaaaatcc ccaaagcaca gcgctggcgc tggatatcgc caacaaaacg ctaccgctgt
      601 ttcgccatgt caatagcgag tcattacgca aggtctgcga aattatccgc gatgacattc
      661 acgccgatgc ggtggcgatt actaataccg atcatgtgct ggcctatgtt ggcgtgggtg
      721 aacataacta tcagaatggc gatgacttca ttagcccgac tacccgtcag gcgatgaatt
      781 acggaaaaat catcattaaa aacaatgatg aagcccaccg cacaccagag attcattcca
      841 tgctggtgat cccattgtgg gagaaagggg tcgtgaccgg aacgctgaaa atttactact
      901 gccacgcgca tcagatcacc tcgtcattac aggaaatggc ggtcggtctg tcgcaaatca
      961 tctccacgca actggaggtt tcacgcgccg agcagctacg tgaaatggca aataaggcag
     1021 agattcgcgc cctgcaaagc aaaattaatc cccattttct gtttaacgct ctgaacgcta
     1081 tttcatcgtc aatccgtctg aatccggata ccgctcgcca gttgatcttt aatctgtcgc
     1141 gttatctgcg ctataacatt gaattaaaag acgatgagca aatcgatatc aaaaaagagc
     1201 tgtatcaaat taaagactat attgccattg agcaggcccg ctttggcgac aagctgacgg
     1261 ttatctatga tattgatgaa gaggtgaatt gctgcattcc cagcctgctg atccagccgt
     1321 tggtggagaa cgccattgtc cacggtattc agccttgcaa aggtaaaggc gttgtcacca
     1381 tcagcgttgc agagtgcgga aatcgggtac gcattgcggt gcgagatacc gggcacggca
     1441 tcgttccaaa ggtgattgag cgggtcgaag cgaatgaaat gccgggcaat aaaattggcc
     1501 tgctgaatgt ccatcatcgc gtgaagttat tgtatggcga ggggctgcat atccgccgcc
     1561 tggagccggg gacggaaatt gcgttttaca ttcctaacca acgcacccca gtcgcctcac
     1621 aggctacgtt attgctttga gccggagtga tattgtgaaa gtcatcattg ttgaagacga
     1681 attcctggca caacaggaac tgagctggct aattaaagag cacagccaga tggagattgt
     1741 cggcaccttt gacgacggtc tggacgtgtt gaagtttttg cagcataacc gcgtcgacgc
     1801 catttttctg gatatcaata ttccgtcgct ggatggcgtg ttgctggcgc aaaacatcag
     1861 ccagttcgcc cataaaccgt ttattgtgtt catcaccgcg tggaaagaac atgcggtaga
     1921 agcgtttgaa ctggaggcgt ttgactacat tctcaaaccg tatcaggagt cacgtattac
     1981 cgggatgctg caaaaactgg aagcggcctg gcaacaacag cagaccagca gtacgcctgc
     2041 cgcgacggta acgcgtgaga atgacaccat taatctggtg aaagatgagc gaataatcgt
     2101 cacgccaatt aacgatatct attacgccga agcgcacgag aaaatgacct ttgtctatac
     2161 gcggcgtgaa tcctacgtaa tgccgatgaa cattaccgaa ttttgcagca aactgccgcc
     2221 gtcgcatttt ttccgctgcc atcgctcatt ttgtgtcaat ctgaacaaaa tacgcgaaat
     2281 cgaaccgtgg tttaataaca cctacattct gcgactgaaa gatctggatt ttgaagtgcc
     2341 ggtcagccgc agcaaagtga aagaatttcg ccagttaatg catctttaat cgggaacttt
     2401 catgaaagca ccaggcttgc ccgccgatca acaatttttc gccgatctgt tcagcggcct
     2461 agtgcttaac ccgcaactac tggggcgcgt ctggtttgcc agccagcctg cctcgttacc
     2521 ggtgggcagt ttatgtattg attttccccg tctggatatc gtgctgcgcg gcgaatacgg
     2581 caacctgctg gaagcaaagc agcagcgaat ggtggaagga gaaatgctgt ttattccggc
     2641 acgcgcggct aatttaccga tcaacaacaa accggtgatg ctgttaagcc tggtgttcgc
     2701 accgacctgg cttgggttat cgttttacga tagccgcacc acgtcgttgt tgcatcctgc
     2761 tcgccagacc cagcttccca gcctgcaacg cggtgaaggt gaagcgatgc ttaccgccct
     2821 cacccatctt agccgttcgc cgctggagca gaatatcatt cagccactgg tgttaagttt
     2881 gctgcatctt tgccgcaacg tggtgaatat gctgccgggt aattcacaac cgcgcggcga
     2941 ttttctctat cacagcattt gtaactgggt ttaggataat tatgcccagc cgctcacccg
     3001 cgagagcgtg gcgcagtttt ttaatatcac gcccaatcat ctgtcaaaac tgtttgctca
     3061 gcatggaacg atgcgtttta tcgagtatgt gcgttgggtg cgaatggcga aggcgaggat
     3121 gattttgcag aaatatcatc tgtcaattca tgaagtggca cagcgttgcg gttttccgga
     3181 tagcgactat ttttgtcgcg ttttccggcg tcagtttagt ctgacgccgg gagagtacag
     3241 cgcccgtttt cagggctaac gtcagaaggt taattctgtt tccagcagcg tcaggatact
     3301 ttgtgcatct tgcgcggcaa acaacgactg gcggaagttt ttattcacca gtttacgcgc
     3361 cagctgcgag aagactttca catgattcat cccttcgtta gcacccagcg tcagcatgat
     3421 caccagttcg acttcgccca tttctgactg ccagtcaacc ggtttcgcca gccgggcaat
     3481 gctgatactg gaatgacgga tccactgaga tttggtgtgc gggatcgcta cgccaaaacc
     3541 aacgccggtg gtaacaatct cttcccgctg ccagacatct tcttccagct caaacggatg
     3601 ttcagtgcgc ccgttaacgc cgaggttgcc gcacaggaac tggatcgcct gctctttatt
     3661 gctaaaatcc tgatcaacaa agatattctc cagcgccagc agtgggcgaa cgtcttcttc
     3721 cggcgtaaag gcggtgagta acgcttcaat ttcctgcgca ctgcggcatt cacatgcctg
     3781 acgcgccagt tcccgacacg cctcgctatc cagttgacga agctggcttt tcaccgccgg
     3841 aatacgcggg ctactcatac tcagctcatc caggcccagc ccaagcagta gcggcagata
     3901 acggctttca ccgcccagtt caccgcaaat gcctacccat ttgccccgct gatgcgcagt
     3961 ggtaactatt tgctgcaaca tgcgcaggaa cgatggcgta atcgggttat atagcggcga
     4021 tacgcgcggg ttattacgat cgaccgcata cagatactgg gtcatatcgt tggagccgat
     4081 actgaagaaa tcgacctcat cgcagaagtg gtcgatgatg tagcacaccg acggcacttc
     4141 caccatgatc ccaagcgtaa tcgtctctgc atgacgcagg ccatcgcgct taagctcaac
     4201 gatcgctttt tggatctcgc ctttcaccca taagatctga tcgaggctgt gaaccatcgg
     4261 gatcatcaac tgggcgttgc cgaaactggc ggcgcgcaaa atggcccgca gttgagtgcg
     4321 gaacaggcca gcaaattccg ggtaaatacg taccgcgcga tagccgagga acgggttctc
     4381 ttcctgggga atattcagat aaggaatgct tttatcgccg ccgatgtcca tcgtgcggaa
     4441 gataatcggc ttgtcgcccg ccgccagtag cacctgctgg taggcttcaa attgctcctg
     4501 ctcgtccggc gcgctgtcgc gatccatata gagcatttcg gtacggaaca gcccgacacc
     4561 ttccgcgccg ttggcaaacg cgcctggcgc ttccagagcg gtgccgatat tcgccgcaat
     4621 atcaatacgc ttgttatcac gggaataggc cagctgcgcg gcagcctgcg cctgttgttt
     4681 ttggcgttta tccgccagcg tctgcgcgac ctgataataa ccgctcaccg cgtcattcgg
     4741 gttaatcgcc agcacgccgc actgggcgtc aagcactgca ggttgcccgg cataacgggc
     4801 aatcgcatcc agcggcaagc cactcagtac cgggatcgcc gaggcacggg ccagaatcag
     4861 tgtatgcgag gtgcgcccgg ttttctccag aatcataccc gcaagatttt tcaaatcaag
     4921 gctcaaaaac tggcttgggg ttaaatcttc agccaccaga atggtcggtt tttccagcac
     4981 cagattgttg cgcggcttca gttccggcca ggtgatatgc agcaactgtt cgctgatatc
     5041 gcgaatgtcg ctgacccgct cgcgcagata atcgctggca gaagcagata gtttggcgca
     5101 aacctgctcc atattgctga tgatcgccgc ccccagtccc tgatgctgtt ctgtcatcag
     5161 gcgacggata ttgcctgcaa attcatcatc ctgaatcagc gacaaatgag cgctgaggat
     5221 agttttgctt tcgccgtcac gctcacgcaa ttgctgattc agttgctcgg caagcgttgc
     5281 caggctgtgc tccagccggg tggaatcttg cgcactggcg gggattgccc gataactgtc
     5341 gaggctgtcg ctttgtaaca gggtcagcgt acccacgccg acgccgcttg ccagcacatt
     5401 gccgtacagt aaatccgggt taaggcggct taatgaacgc ggcagcggat gcgccgtcag
     5461 ttctgcctgc gtcggctgaa cgctgtcgct gtcgataaag cgcaccagga tgtactcttc
     5521 cagcacgcgc cgcgcctgct cttcatcgct gccgctaatg ttcaggctgc aactgtcatt
     5581 aaataaggtg ccagttccaa tcaacgccag cgagctttta gcatcagcct ttgcgttctg
     5641 gcgatggttg ataaaggtga tttcgctttg ccactggctg cactgttctt taagttccca
     5701 cgccggacga gcatgtagac cgttaggcaa aggacagaga aattgaatcg ttaacataac
     5761 cgctccgggt taatcaggag atcatctgaa atccgtcagt tgaacaaccg tctcacgcgt
     5821 aagacgttga ataagggcag ataaaagttg ctccatctgc aaaatgtcgc ggcaatcggc
     5881 aatcgatgcc gcgcaatgtc cgtggcgggt ggcaggcccc attaccaatg tgggtacgcc
     5941 agtaccggtt aagtgcaccg ctccaccgtc cgtgccgccg ttactgaaca tatccgcctg
     6001 caacggaacg ccaatttctg ccgccacggt ttcaatccac gcggtaagtt ttggcggcgc
     6061 aatcagcgat ttatcgctta acaccagcat cggcccgtta ccaatctgac gatggttagc
     6121 cgcgccataa tcaaagtttt tcgcccagca ggcggtatca agcacaatgg cgacgtccgg
     6181 cgacaccgcg cgggtagcag tttgcccgcc gcgtaatccc acctcttcgc tggaacttgc
     6241 caccagccac acttccgcag gcagttcggc gtcgtgcagc tcacgtagta acgtcaccag
     6301 cagatagcaa ctgaggcgat catcaaaggc tttgcccatc acccgctggt gagggaggac
     6361 ctgaaaagtg gtatcgaagg tgacgcgatc gcctggacga attcccgcct gcatcacttc
     6421 gtcataggtg cgcgcgccaa tgtccacgcg catggcgcta acgtcattcc cctgccggtc
     6481 gccgtcaagc aggcctggaa ttttgcactc ttcacgggtg gtgatgcgca ccggctgcag
     6541 ctggcgggca gccatgcgca cgttgccaac cggcagcacg tcaatagcgc cttcgcggga
     6601 gatgctgcgc accataaatc ccacttcgtc catatgggcg cagatcatca ccttcggacc
     6661 tgtcgattca ttgaggcgga tcagcaccga tcccagacca tcaaatcgca cttctttttg
     6721 caggcgagcc gcttcttcca gcaggatctg ccgcacttcc tgttccgagg aggcgatcgc
     6781 atctgcctcg ctcaacgctt ttaatagcga taaatccatt atgcctctcc cgtgagcaac
     6841 actgttttcg gcatggcgta gagcacttct gcgccttgcg gggtaaccag cacaacatct
     6901 tcgatgcgca cgcccccttg ccctagcaaa taaatccccg gctccacggt cagcaacatg
     6961 cctggctgta gcgtcgtggt gtcccgcggt gaaaaacgcg gatcttcatg gacttcaatg
     7021 ccgatagcgt gaccagtgtt atgaccgaaa tagtcgccaa atccagcctc ggtaatgacc
     7081 cggcgcgcgg cgtcgtcaat ctgctggcag cgcacgccgg ggcgaattgc agagattgcc
     7141 gcgagctgtg cctgcaggac aatctgatag acgtcaaaca gcgggtgaga ttcggcgctc
     7201 accccttcgc cattcaccag caaggtgcgc gtcatatcag cagtagccct gatacagcgc
     7261 accaaaatcg agagtgacaa actcgcccgc tgcaacaatc ttgtcgctgg ctttgccgtg
     7321 cggcagcgcc ccgcgccagc cgctggcgac aatggtgtca aaagaggctt tttctgcgcc
     7381 ctgctggcgc ataaaccact ccagttcagc ggctatctcg cgctcgctca tccccgcctg
     7441 aataaagcgg cgaatatgct ccgctccgcg atcggcaatc ccacaggcga ggcggattat
     7501 ctccacctcc tctggcgttt tgatttgccg cagcacatcc ggcgtggcgc taaccagttt
     7561 cgcattgagt tcagacttcc agcggtgcgc ggtttcccaa ctcacctgct ggccttcaaa
     7621 accgagcgtt tgcaactgct catcggcaat gatttgattg acgatagtgg taagcgtgtt
     7681 cgttgcgtca agcaaatgca gctggtagcc ttgcgcacgg gcttccacct caacgaaata
     7741 gcgcgaatct accagaatgt gcgcactttc acggctaata accacatagc ctgatccggt
     7801 ggagatcccc agatgcggct gtttgttctg ccgtgaggag agaagcaccg catccagttg
     7861 ttgcacctta agccagttgc gcagcgaagc gagtaatgtc atctggattt tccttcttac
     7921 aggctatcga ttaacaattt gcctttgcgg aacatcatca gacgcaggaa caccaccatc
     7981 agcgcagtaa tgattgcccc cagcgcgatc cccgccatat aaacgcccaa gttagtgacc
     8041 agcggccatg cccagatagc ggattccggg aaccattgca ctgcgcccag ccagacagct
     8101 gcggttgagc cgacaatcgc gccgaccata tatgacggaa tagcggtgat ggggctttcc
     8161 agcgcaaacg gaatcgcacc ctcactaatc cccataaagg ccaggaacat cgcggtctta
     8221 ccctgcggat aaagttgagc gttgaacagg cgtttgccgg ttaaacgacg gtcaataatg
     8281 gtcgccagac ccagaccaat cggcggaata acgatagcga tagaacgagc ggtgaccggt
     8341 aatacgtggt cagtggtaaa gctgaaggca acgaaacctg cggctttgtt gatcgggcca
     8401 ccaaggtcga ttgctgtcgc cgcggcgatc cccattgcgt acatcagcgc gcccttctca
     8461 cctgcggcag tcagcacagt acggataccg ccgttgatcc agccaccaaa gggagtgatg
     8521 acgtagtaca tcgccagcat gacaaaaatg gcggaaagaa tcggtagcaa aaatgtggtt
     8581 ttgaacgcta acaggaaatc cggtaactgg attttctggt tcatccattt cacaaggtaa
     8641 ccggcaacga tagaaataat cagcgcacca atgaaggtgg acggcaccgg cgaagaggtc
     8701 gcccattgca tagtgctggg atcgaagttc agcagttgcg tcggctgggt agacatcaac
     8761 ccgccgataa aaccagccgg gaacgccagt ttgccaccga tagagttcgc cacaaatgca
     8821 gcaaacatcg gaatggcaaa accaaacaac acgccgccga aggactgcga cagccaggca
     8881 aatttaagta aggagaggtc gaaaccggag aatttcccgc tattaagagc atccatgatg
     8941 ccgatctcag caggaatttt aagccagcta taagcaatca gctggctgaa agcgaggatc
     9001 acaccgccca taattaaggt cggcaccatg cgtgaaatcc ctgacatcac atgctgcggg
     9061 agttcacccc agaagctgct tttagaggcc tgcggattct taactgccgc tgccgcaccg
     9121 gatgcgccag gcacaacggt tgcactgcgt tttttaatgg ccataacaat tatccctgtc
     9181 gaatattact gttgttcaga ggcaatcatc tcttcgattt ctttgatgat gcccgcagcg
     9241 tttttaattg cgtcctgcaa agtgatttca taaacgtcgc gtgattcgaa acgttcgtta
     9301 tcttccgggg taactgccac ggaatggatg atgatggtcg cttcggcgat atcctgcgcc
     9361 gtcaggcgat tctggatgcc gtccgcgccc tgggtttcaa ttttcacttc ataaccggct
     9421 tctaccgccg cttcttccag cacctgagcg gccataaaag tgtgagccag gcccattggg
     9481 caggcacata aggcaatcag tttcttactc atcgttaatt cctcgttcag tgatagttga
     9541 ctagtgaaat tatgaacgtg caaaacaaat cgccgttacc agacaaataa tgcatttact
     9601 ggagaattaa tcccgctgta cggaagtgtg acgcaggtcg gccttgtgag gggacacaaa
     9661 gaaaaattga attaactgta tgatttaaaa gattatcggg agagttacct cccgatataa
     9721 caggaaggat ttacagaatg tgacctaagg tctggcgtaa atgtgcaccg gaaccgagaa
     9781 gacccggata gtcatggacg atgagataca ccggaatatc atggacatat tctttaaagc
     9841 gccctttatc ttcaaatgcg gcacggaaac cggaggcttt gaagaactca aggaagcgcg
     9901 gcacgatacc gcccgcaata aacacgccgc caaatgtccc gagattgagc gccagattgc
     9961 cgccaaaacg gcccataatg acgcaaaaca gcgacaatgc gcggcggcaa tcggtgcagc
    10021 tgtcagccag cgcgcgttcg gtaatatctt ttggcttgag attttctggc aggcggttgt
    10081 cagctttcac aattgcgcga tacaaattca ccagcccagg gccagaaagc acgcgctccg
    10141 ccgaaacatg accaatttcc gcacgcaata tttcgaggat aatggcctct tcttcactat
    10201 tcggcgcaaa atcaacgtga ccgccttcgc ccggcaagct tacccaacgc ttatcgacat
    10261 ggaccagatg cgcaacccca agccccgttc cggcaccgta aaccgcaata ggcttacctt
    10321 cgaccggttc tgcgccacca aactgaatca gatgctcttt tttcagcatc gggatcgcca
    10381 tcgatacagc ggtaaaatcg ttaataattt ccagatggct aaaaccgaga ttctttttca
    10441 tttcggcaat tgagaacgcc caggtatggt tggtcattgc cacccagtca ccggtaattg
    10501 ggcaagcgat ggcaatacag ccgtctttca cctcgacctt atgttcttca agataaacgc
    10561 gaatgaccgc ttcgaggctg gggtaatcaa gccctgaata ggtcttagcc tgcgagattt
    10621 caccactggc aatatcacac agagcaagac gtgcgttggt gccgcccaca tcaccgacta
    10681 atgcatactt tgtcattctt caactgctcc gctaaagtca aaataattct ttctcacact
    10741 gtaaatacct gggggcataa caacaacgcc ggaaaggcag gctccctgta aatatcgatc
    10801 tgggtcacac aattacttta tcgtttcagc accaattgca gcgatgcctt tttgcaagct
    10861 gggcaaacta agtatctgac cccgcataag gaatagaaca tgctccatcc gcgagccaga
    10921 accatgttgt tattatcgct ccccgccgtg gcaattggga ttgcgtccag tcttattctg
    10981 attgtggtga tgaaaatcgc ctcggtatta cagaatttgc tctggcaacg actgccggga
    11041 actctgggga tagcccagga ttcacccctc tggatcatcg gtgtattaac gctaacgggt
    11101 attgcggtgg ggttggttat ccgtttcagc cagggtcatg ccggaccaga ccccgcctgt
    11161 gaaccgctga tcggcgcacc ggttccgccc tctgcgctac ctagacttat cgtagcatta
    11221 attctcggtc ttgctggcgg cgtcagcctg gggccggaac atccgatcat gaccgtcaat
    11281 atcgcccttg ccgtcgccat tggcgctcgt ctgttaccgc gcgtcaaccg aatggagtgg
    11341 actattttag cctctgccgg aaccatcggc gcgctgtttg gtacacctgt tgcggcggcg
    11401 ttgatatttt cgcaaacctt aaatggcagt agtgaagttc cgctatggga tcgtcttttt
    11461 gcgccgttaa tggcggcagc agctggtgca cttactaccg gattattttt ccatccccat
    11521 ttttcactgc ccattgcgca ttacggacag atggaaatga ccgatattct cagcggtgca
    11581 attgtcgcgg cgattgccat cgcagcaggg atggtcgccg tatggtgctt accacggttg
    11641 cacgcgatga tgaatcaaat gaaaaatccg gtgctcgtgc tgggtattgg cggatttatt
    11701 ctcggaattc tgggggttat tggtggacca gtttcgctgt ttaaagggct ggatgagatg
    11761 cagcagatgg tggcaaatca ggctttcagc accagcgatt actttttgct ggcggtaatt
    11821 aaacttgccg ccctggtcgt tgctgccgcc agtggctttc gcggtgggcg aatcttcccg
    11881 gcagtgtttg tcggcgtggc attagggttg atgctgcatg agcacgttcc cgccgttcca
    11941 gcggcaataa ccgtttcctg cgctattctc ggcatcgtgc tggtggtaac acgcgatggc
    12001 tggttaagtc tttttatggc ggcagtcgtt gtacccaata ccacattgct accgctgctc
    12061 tgtatcgtca tgcttccggc atggctgtta ttagcaggta agccgatgat gatggtcaat
    12121 cgtccgaagc aacagccacc ccacgataat gtttagcaaa aaatgctctc ctttatgatt
    12181 taagagttat ggcgatgata cggggcttta cctccccgta atattgcgtt aacaggccgc
    12241 tgacggcttc gatcgtgaag gagaataaca atgttcaggt cactgtttct ggcggccgcc
    12301 ctgatggcat ttaccccgct tgcagcaaat gcaggtgaaa tcaccctact gccatcgatc
    12361 aaattacaaa ttggcgatcg cgatcattac ggtaattact gggacggtgg tcactggcga
    12421 gatcgtgatt actggcatcg caattatgaa tggcgcaaaa accgctggtg gcgtcatgat
    12481 aatggctatc atcgtggctg ggataagcgt aaagcatatg agcgtggcta tcgtgaaggc
    12541 tggcgcgatc gtgacgatca tcgcggaaaa ggccgcggac atgggcaccg ccattaaacg
    12601 cgtcctccaa tggagcacaa tgcctgatgc gacgctagcg cgtcttatca ggcctacaaa
    12661 ccggctcttt aacgcgtagg gcggataagg cgtttacgcc gcatccgcca atagtgccag
    12721 atgcgacgct cattcaacta caatcccagc gccgtcccca ccaacaacca gatattcagc
    12781 gccacgacca gcactacaat cacccagcct gtctgtttta cgcgtttgct gttcaccaga
    12841 tcgcccatca acttgctgtc actggtgaaa atcagcagtg gaaccagcgc cagggcgata
    12901 ccaaaactta acagcacctg actcataacc agaatccgtg tcggatctaa tcccatcaga
    12961 atgacaataa atgacggcaa catggtgact gtacgacgca cccacagcgg gatatggaag
    13021 cgaatgaatc cctgcatcac cacctgcccc gccagtgtcc ccaccaccgt tgaggacagt
    13081 ccggcagcaa ccagacttaa cccaaagacc gttgccgcag catggcttaa cagcggttgc
    13141 agcgtcagat aagcctcatc aagatcggca acaccagtat gaccagaaaa gtggaacgcc
    13201 gccgcagctg tagccatcat caccagattg acaaaaccgg caatcgtcat ggcgatagcc
    13261 acatcccatt tggtggcgga ataacgttgt tgacgcgaac cgccatgtaa atgctgagtg
    13321 agcgaagagt gcaaataaat cacatgcggc ataatcgtcg cccctaacac gcctgctgcc
    13381 aggaagaccg cctccgaagt aggtaaactc gggatcacca ttcctttacc cagctgcgcc
    13441 aggttaggct gggagaaaat caactcgaca atgtaagccg cggcaacaaa caacagtaac
    13501 ccgccaatca ctttctccag cggtttttgc ccgcgacgtt gcagcattaa aatcaggaaa
    13561 gtcgcgatcc ccgtcagcac cgcgccctgc aacaacgaaa caccaagaat gagtttaaaa
    13621 ccgatcgccg caccaataaa ttccgccagg tcggttgcca tcgcaataat ttctgcctga
    13681 acccaataga accacactac gggacgcgga tagtgatcac gaatctgctc cgccagattt
    13741 ttaccggtgg caatccctag tttggcagag aggatctgaa tcagcatcgc catcaggttg
    13801 gcccaaacga caacccacag tagctgatag ccgaagctag cacccgcctg aatattggtc
    13861 gcaaagttac cgggatcgat ataaccaatc gccgcaatga acgcaggtcc cattaatgcg
    13921 agcctcatct tacgcgccgc ccgtccgctg ctactctcaa cgcgatagtt cgtcatcttg
    13981 tgcctctaaa acatagcctt tgctatgttt catgctatgc taaacgagaa tgattatcaa
    14041 attcatttaa atggattgtg gtgatttctc tgatagacca agattatgat tacgaaaaga
    14101 ttgatgactt gaatgttatg gttatgttta atgttagcac atttacataa ctttcagctt
    14161 ccatacacaa catagcagaa atgtatgaca gatcactatt tttgaagcct gtcacaggac
    14221 gtcattatag tgtgtgtcag atctcgtttt ccttaaccat gttacataga atgtgcacgg
    14281 aaatttaacc tgcctcatat ttggagcaaa tatggaccgc gtccttcatt ttgtactggc
    14341 acttgccgtt gttgtgattc tcgcactgct ggtaagcagc gaccgcaaaa aaattcgtat
    14401 ccgttatgtt attcaactgc ttgttatcga agtgttactg gcgtggttct tcctgaactc
    14461 cgacgttggt ttaggcttcg tgaaaggctt ctccgaaatg ttcgaaaaac tgctcggatt
    14521 tgccaacgaa gggactaact tcgtctttgg tagcatgaat gatcaaggcc tggcattctt
    14581 cttcctgaaa gtgctgtgcc caatcgtctt tatctctgca ctgatcggta ttctccagca
    14641 cattcgcgtg ttgccggtga ttatccgcgc aattggtttc ctgctctcca aagtcaacgg
    14701 tatgggcaaa ctggaatcct ttaacgccgt cagctccctg attctgggtc agtcagaaaa
    14761 ctttattgcc tataaagata tcctcggcaa aatctcccgt aatcgtatgt acaccatggc
    14821 tgccacggca atgtccaccg tgtcgatgtc catcgttggt gcatacatga ccatgctgga
    14881 accgaaatac gtcgttgctg cgctggtact gaacatgttc agcaccttta tcgtgctgtc
    14941 gctgatcaat ccttaccgtg ttgatgccag tgaagaaaac atccagatgt ccaacctgca
    15001 cgaaggtcag agcttcttcg aaatgctggg tgaatacatt ctggcaggtt tcaaagttgc
    15061 cattatcgtt gccgcgatgc tgatcggctt tatcgccctg atcgccgcgc tgaacgcact
    15121 gtttgccacc gttactggct ggtttggcta cagcatctcc ttccagggca tcctgggcta
    15181 catcttctat ccgattgcat gggtgatggg tgttccttcc agtgaagcac tgcaagtggg
    15241 cagtatcatg gcgaccaaac tggtttccaa cgagttcgtt gcgatgatgg atctgcagaa
    15301 aattgcttcc acgctctctc cgcgtgctga aggcatcatc tctgtgttcc tagtttcctt
    15361 cgctaacttc tcttcaatcg ggattatcgc aggtgcagtt aaaggcctga atgaagagca
    15421 aggtaacgtg gtttctcgct tcggtctgaa actggtttac ggctctaccc tggtgagtgt
    15481 gctgtctgcg tcaatcgcag cactggtgct gtaaaaccat acataaaaaa gccggggatc
    15541 attccccggc ttttttacgc ctgttaatca gctaatggct gcgggcgacc aatcaaatac
    15601 ccttgcagat attgcacccc gagcttatgc aatagcgcct gctgctgttg cgtctcgaca
    15661 aactccgcga ccacactcaa tgacttcgct ttcgccagat cggtaattga tctcacaatc
    15721 atcgcatcca gcgtattcgt gacaatatct ttcacaaaga cgccatcaat tttgatgata
    15781 tcagcctgca aacgctttaa ccgttcgtag ttggcatatc cggtgccaaa gtcatcaatc
    15841 gcaatccgga aaccaaactt atgcagctgc tcgatgttgt acatgctgct ttctgcgtta
    15901 gaaaacgcct gctcctcggt gatctcaaga atgaccgcct gcggggagat gtgataacgc
    15961 ttaaacagac gaataatccg cccggcaata ttcttttgca gcagcgtgag cggcattaaa
    16021 ttgactgaaa agcgcggtcc ttttttgtcg caagggtgtg ttgccagcca cttcaacaag
    16081 gattccagca cttgcaaatc aaaacgcgcg ctaaggttaa actgagcaat aaggggcaga
    16141 aacttatccg gggtcataat gccgccgtca tatttcagtc gcgcgaggat ctcatcataa
    16201 ccttcaccct ctttgttgcg aattggctgg gcgtagagca gcaaatcacc ctgatctaac
    16261 gccgtgcgaa tggtattcag caatagcacc tgtttagtgg tctgcccgga aaccatctcc
    16321 tctctgctat ccagcgccag cacatgatga tgtgcgcagg attgctccgc cagccagctt
    16381 aactgcccca acaagggttg cagggtttcc tgatttccat caaaacgccc ccaggcagca
    16441 ccatagccca tatccagccc ggtattgttc cagtgaattt gccgactatt caggatgtta
    16501 accatatgct ggagtcgccc ttccgtttcc ggcccgctca gcactaacag cagttcacta
    16561 cccggcaatt gatacaactt ttcgttttcc tgcatcaacg gcagcagcgt acggcaaatt
    16621 gagcggatac agtgaacgcg catcattaag ccgtaatgac gactcataaa ctcaagatta
    16681 tcaatgcgca ggcagcaaaa actcttgccc gcctcttgct ccggcgcttg ttccaacgca
    16741 cgaaagttgg gtaggagcgt taacggatct gttagcgcct gcaaatgcca gcggcgatta
    16801 agccattcac tgcgatgata aatgcgcacc atatagagca ggcaaacgct aaaggaaatc
    16861 agcaccgcga gaataaatgc cagcgaatat tcggtttcca ccccttgcaa aaagttctga
    16921 ttgtaattca gaaggcaaag cgttgaaaca gcccaggtga gatttaaaaa cggatagcga
    16981 agattaccga ccccgagggt aaagatgatg aagaaaacgg gtaccaggta accggcaata
    17041 aagtcgtttt cataaggtgt gcacaacaga agtagcagca cgcttaacgc tgccagccag
    17101 cttaaggtaa atgcgagtga gatagtagaa aagcatgttg taaatcagca ctgcggtgaa
    17161 aaggcttagt aaatcaacga ccgtgaaaat ggcatccgca tcgccgaaaa aggtggatat
    17221 ctttagcggg aaatcaaaga agctccccac aagatacatg ctgcatttga tgccgatcgg
    17281 cgtcaccaaa ccaagccaga acagacgttg ccagacatat cggctggtca atccataacg
    17341 ccaacgcgtc cccagctgcc agcgtaatat tgcgcaggca cacaacacga caaacgtctg
    17401 gctgccgagc atgaccgcag tctgtaaaaa agagagatga aagttccaca gattggtgca
    17461 aaacatcccg cataaaacgg gaacgacacc gcgccagcca aagataaaca agattgacag
    17521 cattacgcac agtggcatcc acgccagaaa gatataactg gaatggataa tggcaagtgg
    17581 cgaaataaaa cgggatagct gaataagtac cacggtgagc gtaaacgcta gtgtgaatat
    17641 cttgatattt tttatcaggt tatgctccac aaacatgagt acactactct cactgatcgg
    17701 ggttttcagg cacacaaata ataagtttat tcgcatcgcg aagcaaacag gggccagcct
    17761 gttgccgatt tttcatccaa tgatgctttc accaggcggc gctaacccgg acgtagactg
    17821 tattacaaaa gcggcaaaaa gcagagacaa aaaacccccg ctttgcagcg agggttggaa
    17881 atttggtgga gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc
    17941 ccagctgagc tatagcccca cgatgcgttt acgtaccaaa tttgctgggt gcaaaatttg
    18001 gtggagctaa gcgggatcga accgctgacc tcttgcatgc catgcaagcg ctctcccagc
    18061 tgagctatag ccccgtcacg taaagcttgt cgagttgacg ggcggcatca tatgaattcc
    18121 gcccgaatgt gtcaacggca aattgcaacg tgtagtttca atcgctgaaa aatcaggcaa
    18181 atgaacaatt ttggaaaacc gctcgcattc agtagttatt catgtcacgg tttcctgtaa
    18241 agtggtgtta taaaatgaac tactaataga cccacataca ttcagggaat tgttatgttc
    18301 aaggagagga tgacaccaga tgaacttgcc agactgaccg gctatagccg ccagaccatt
    18361 aataaatggg tacgcaagga aggctggaca acgtcaccaa aacctggcgt ccagggtggc
    18421 aaagcccgac tggttcacgt caatgaacag gttcgtgaat atattcgcaa tgctgaacgt
    18481 ccagaaggtc agggagaagc gcctgccctt tctggtgatg cgccgcttga agtattgctg
    18541 gtgacactgg cgaaagagat gacgccagtt gaacaaaaac agtttacatc cttgcttctg
    18601 cgggaaggga ttatcggatt gttacaacgc ttagggattc gcgatagcaa ataatatgaa
    18661 aagattacgc aataaaatga ccaccgaaga actggctgaa tgcctcggtg tggctaaaca
    18721 aactgttaac cgttggacca gagaaaaagg ctggaaaacg gaaaaatttc ccggcgtgaa
    18781 aggcggtcgt gccagactta ttctggtcga tacgcaagtt tgcgagttta ttcagaacac
    18841 gccagccttc cataacacac caatgttgat ggaagctgaa gagagaattg ctgaatacgc
    18901 tcctggagca cgcgccccgg cgtatcgaca aatcatcaac gcgatagata atatgacaga
    18961 cattgagcag gaaaaagtcg cgcaattttt gtcacgtgaa ggaattcgca atttcctcgc
    19021 ccgtctcgat attgacgaat cagcataaat aaaaaacggc aggatattat ctcctgccgt
    19081 ttatcttttt acacgctaat tactgctgat tttcgcgttc agcaataaaa tccagcgctt
    19141 tgttgatacg ctcgatactg cgggtcttac caattgcgtg aacggtaaca tccagtgctg
    19201 gagactgccc cgcaccggtt acggcgacac gcagcggcat accaacttta cccataccca
    19261 cttccagctc atccgccgtc gcctgaatag cgtgatgaac gttttcagcg gtccagtcag
    19321 taatcgcggc cagtttgtca cgaaccactt ccagcggctg acgcgctacc ggacgcagat
    19381 gttttttcgc cgcatcggca tcgaactcag caaaatcttc gtagaaataa cggcagctct
    19441 gtgccatctc tttcagagtc ttgcagcgtt cgcccagcag tttcaccaga tcagccagct
    19501 gcgggccgtt acgggtatcg atattttcct gctcaatgtg ccactgtaag tgagtagcaa
    19561 catactccgg cggcagcgcg ttaatgtagt gatggttcag ccacagcagc ttgtcggtgt
    19621 tgaacgcact ggcagatttg ctgacggcat tcaaagtgaa gtatttgatc atctcttcac
    19681 gagtgaagat ttcctgatcg ccgtgggacc agcccagacg caccagatag ttcagcagtg
    19741 cttctggcaa ataaccgtca tcacgatact gcattacgct gactgccccg tgacgtttgg
    19801 acagtttttt accgtcatcg ccattgatca tagaaacgtg cgcgtaaacc ggcaccggcg
    19861 ctttcagggc cttaagaatg ttgatctggc gcggcgtgtt gttgatatgg tcttcgccac
    19921 ggataacgtg ggtgatttcc atatcccagt catcgacaac cacacagaag ttataggttg
    19981 gggaaccatc ggtacggcgg ataataagat cgtccagttc ctggttgctg aactcgatcg
    20041 gaccacggat ctgatcgtca aaaacaacag aaccttcctg cgggttagca aaacgtacaa
    20101 cacacggttc atcatcagca tgatgctcat ggctgtggcg gcagcgaccg tcataacgcg
    20161 gcttctcacc tttcgccatt tgctcttcgc gcagcgcttc caggcgctct ttagagcaat
    20221 agcatttata agcagtgccc tcttccagca tctgatcgat caccgcgttg tagcgatcaa
    20281 aacgtttggt ctggtagtac ggaccttcat cccactccag gctcagccag ttcatgccat
    20341 ccataatggc ttcgatagct tccggcgtgg aacgctcaag atcggtgtct tcaatacgca
    20401 gcacgaactc accgccgtgg ttacgtgcaa aaagccagga gtaaagagca gtacgcgcgc
    20461 cgccaacgtg cagatagcct gttgggcttg gcgcgaagcg agttttgatt ttcatgaaat
    20521 ggccttacgt ttagaaagat gccgacaacc ggcaaatcct ggaaaaattg agtgggcgat
    20581 attctatcac tcccgcctga ttcctcaatg tagttcgggt ttctacctta cgcattattg
    20641 ttttcgttga caaattgcgc aacacggtct gttttgcgat catcttgctt aaatttacga
    20701 cgaacgaaca atttctttag aaaatgcgtt gactcatttt gaactctccc tataatgcga
    20761 ctccacacag cgggggtgat tagctcagct gggagagcac ctcccttaca aggagggggt
    20821 cggcggttcg atcccgtcat cacccaccac ttcgggtcgt tagctcagtt ggtagagcag
    20881 ttgactttta atcaattggt cgcaggttcg aatcctgcac gacccaccaa tgtaaaaaag
    20941 cgccctaaag gcgctttttt gctatctgcg atactcaaag attcgaacct gcagcaggtt
    21001 tgagttgagc gcagcgaaac aacggagccg ctcgcggcga cggcccgaag ggcgagcgaa
    21061 gcgagtcatc ctgcacgacc caccaatgca aaaaagcgcc ctaaaggcgc ttttttgcta
    21121 tctgcgattt gtaagattga tttttcttct caagttcctt gttcttctac cgatattacc
    21181 tcgtacagtg acgaggactg aacaatggcc actatctctt caacttcaat tccttccata
    21241 caaacacaga gtagtaacag ggcatcacaa ggtagcgatg ttgcttcaca aatcgcgcgg
    21301 ataagtcagc aaatcatcaa actcactcaa caaattaaag agattgtaga cactagcggc
    21361 agtgctgaag acaagcaaaa acaggccgaa ttgattcaac agcaaatcac cctactggaa
    21421 acacaactgg cgcagttgca gaaacagcag gcagaaaagg cgcaggagaa agagcagcgt
    21481 ttgtctctga atgtatccct tctaaatcct gttgaaaaaa cgactcatat cgatatctat
    21541 atctgataca acggatcccc ttcccgacca gccattagcg cattgccctg gtctcgtacc
    21601 acgtcccata acgcttcagc ggcggtggaa agtgaacggt tcttcctgcg caccagcatc
    21661 aactggcgtt caaccaccgg cgtaatgcgc ttaaccacca gtgggctacc ttcaggcaat
    21721 ggtagcgcca gtgcaggaag aatactgatg ccgatgcctg cggctaccat cgggaacagc
    21781 gtcgccggat gtccaatctc ctgcacaata ttcgcctgaa taccattgcg cgccagcgct
    21841 gcgtcaatca gcggtcggct gcctgacgcg taatcctgca acaccaattt cgctccctgt
    21901 aatgcctgcc agggtacata atcttccacg gccaaagcgc tatcgcgatg gcaaagcaga
    21961 aagaaaggct cggaaagaat cgcttcacat tgcagatcgc ccactgggcc tggatcgatg
    22021 acgatgccaa agtcgacatc tccctggcga atactttcca tcacccactg ctgcggacga
    22081 tcatgcagga caaactgaat atctggatag cggcgatggc tttcagcaat gcattgcgga
    22141 ataagatgcg cggaaatggt ctggctggca gcgacccgaa cttttccgct cagttgttgc
    22201 cccatacgcc cggtatcgcg caacgtgctg ttaagttcat ccagcagtcg ctcaagacgc
    22261 aaagccaact gctgccctgc atctgtaagc accacttcac gcgtggttct gtccagcagg
    22321 cgaacaccag tatgattttc cagctccttc acactgtgac ttactgccga ctggctcaag
    22381 ccgatacgct ctcctgcacg actaaaactt ttctcctgcg ctactgtgac gaaaaccttt
    22441 aattgtttta aagaataatt catctgtttt cttcatgaat ggatgcaata aatcaatttt
    22501 atttctcaaa ctggaagaag cacaatagaa ccatcgatca tctggagtct ttatgaaact
    22561 ttttcgtatc ctcgatcctt tcaccttaac cctgatcacg gtggtgttgc tggcctcttt
    22621 ctttccggcc agaggcgatt tcgtcccctt ctttgaaaat ctgaccaccg cagctattgc
    22681 cctgctgttc tttatgcacg gcgcgaagtt gtcgcgtgag gcgattattg ctggcggtgg
    22741 tcactggcga ctgcatttgt gggtaatgtg cagcaccttc gtgctgtttc cgattctggg
    22801 tgtactgttt gcctggtgga aaccggtaaa tgtcgacccg atgctctact ccggttttct
    22861 ctacttgtgc attctcccgg ctaccgtgca gtctgcaatc gccttcacgt caatggcggg
    22921 cggtaacgtc gcggcggcgg tttgttctgc gtcagcatcc agcctgctgg ggattttcct
    22981 ttcaccattg ctggttggtc tggtgatgaa tgttcacggt gcaaggggca gccttgagca
    23041 ggtcggtaaa attatgctgc aactgctgct gccgtttgtg ttggggcatc tttcccggcc
    23101 gtggattggt gactgggtgt cgcgcaataa aaaatggatt gcgaaaactg accagacgtc
    23161 cattctgttg gtggtttata cagcgttcag cgaagccgtc gttaatggta tctggcataa
    23221 agttggctgg ggatcattgc tgtttatcgt ggtggtcagc tgcgttcttc tggctatcgt
    23281 gattgtagtt aacgtcttta tggcacgccg actgagcttc aataaggcag atgaaattac
    23341 tatcgtcttt tgtggttcga aaaagagtct ggcaaatggc atcccgatgg caaacattct
    23401 gttccccaca tcggtgatcg gtatgatggt gctgcccctg atgattttcc atcagatcca
    23461 attgatggtc tgtgcggtgc tggcgcgtcg atacaaacgc cagaccgaac agttacaggc
    23521 gcagcaggaa agcagcgccg ataaagctta aagcggacgc ttcaggggct ggaccagttg
    23581 cgtcagcccc tcggttttaa tcagcagcgt gatttgcatt agctcaccga gcttccctgc
    23641 cgggaactca tctttgcggg caaaccacag cagatactcc tccggcaggt caattaagcg
    23701 acgccctttg tatttgccaa acggcattat cgtattggct atttcaatca gctgctcttt
    23761 ttccatctca gctacccagc aaacgcagca tttccgcttc gtcgatgact tcaatgccca
    23821 gttcctgcgc cttcgccagt ttagatcctg cagcttcacc cgctatcacc agatcggttt
    23881 tcttcgacac gctgcccgcg actttcgcgc ccagttcgac cagtcgagct ttagcgtcat
    23941 cacgcgacat ctggcttaag ctgcccgtaa gcaccacggt tttaccagca aacgggctgt
    24001 caatctcttc cgcgttgata acgatcggcg caggccagtg aacaccttcc gccaacagct
    24061 cgctgatgac attgcggttg ctttcttcgg caaagaagtt gtgaacgtgg gatgcaacga
    24121 caatgccaac atcaggcacc ttttgcagct cttcaatcga agcggcttcc agcgcttcca
    24181 gcgtgccgaa atatgccgcc agacctgctg cggtggcctc gccgacttca cggatgccaa
    24241 gtgcatagag gaagcgagca aaggtggttt ctttcgcttt ttccagcgcg ttaaccacgt
    24301 tttgtgccaa ttttggcccc atacgctcca gtccggtcag tttgcctgcg gtgagtttga
    24361 acagatctgc cggagtatgg acatattctt tttcaaccag ctgatcgatg attttgtcgc
    24421 ccattccgtc aacatccatc gcacggcggg aaacaaagtg tttcagcgac tctttacgct
    24481 gcgcaccgca aatcaggcca ccggtacagc gggcaaccgc ttcaccttcc acacgctcaa
    24541 cgtcagaacc acataccgga caatgcgtcg ggaatacaac ctcacgggta tcttccgggc
    24601 gttcagaaag cacgacgtta accacctgcg ggatcacgtc gccagcgcgg cgaatcacca
    24661 ctttatcgcc aatgcgtaaa ccaagacgtt cgatttcatc cgcattgtgt aaggttgcgt
    24721 tactcaccag cacgccagca acatggacag gttccagacg cgcaacaggc gtaatcgcgc
    24781 cagtacgccc aacctgaaac tcgacgtcac gcacaaaggt catctgctcc tgcgccggga
    24841 atttaaacgc aaccgcccag cgcggggcac gcgcgacaaa gccaagctgc tcctgctgtt
    24901 ccagtgagtt gaccttaatc accacgccgt cgatatcaaa gcccagcgtc gggcggtctt
    24961 cttccacttt gtgatagaac gccagcactt cttccgccga ttcacaaagc gttacccgat
    25021 cgctgaccgg caccccccac tttttaaatt gcagtaaacg gccaagatga gtatccggca
    25081 gctcgccacc ttccagaaca ccaacgccat agcagaaaaa agtgagcggt cgcttcgctg
    25141 taatacgcgg atcaagctga cgcagtgaac cagctgccgc attacgtggg ttagcaaaaa
    25201 ctttcccgcc cgtgcgtcgc gcatcttcgt taatcttttc gaaccccgcc tgcggcagga
    25261 acacttcacc acgcacttcc agacgcgccg ggatattctc tccgtgcagc ttcagcggaa
    25321 tggcgcgaat agtacgcaca ttagacgtga tatcttcccc ggtggtgcca tcgccacggg
    25381 tcgcggcact gactaaaacg ccattttcat acagaatact gacggcaagg ccatccagct
    25441 tcagctcaca gcaccaggtg actttctcgt tgtttttcag acggtcctgc acacgtttgt
    25501 tgaaagcaag aaagctttct tcatcaaaaa cgttatccag tgacagcatt ggtacttcat
    25561 ggcgtatctg gctgaaagct gccagcggcg cagcacctac acgttgggta ggcgaatcag
    25621 gcgtaatcag ttctggatgt ttggtttcca gctcgcgcag ttcgcgcatc agcctgtcgt
    25681 attcagcgtc gggaatttcc ggcgcatcca tcacatgata aagatattca tgatggcaaa
    25741 gcgtcgttcg cagttctgtc agttgttgtt cgattgattc catatcgcac catcaatgct
    25801 aaaaaccccc gacaagcggg ggttcgaaga ggagttaatt tgccttaagt gtatcaggcg
    25861 ttggcgtctt tgacttcgcg gatgatgtcc tggtactcgc gcaatttctg cggagtcatc
    25921 atacggcgct gatcgtcaag cacgacaccg cccacttcat cggcaatatg ctgcgcagat
    25981 tgcagcatca gcttgaagtt ctgcagctcg tcaccgtaag acggtacctg cataaagata
    26041 gtgacacccg gagtagtgaa atccttcatt tcaggatcaa aggttcccgg tttcaccata
    26101 ttcgccaggc tgaataacgc cgggccgctg ccatccgggc taagatgacg atggtaaata
    26161 ttcatatcgc caaaaatgaa gcccgcttgt tgaatgctgt taagaagcag ttcaccgttt
    26221 agctcgctac cgtgatgcgc cgcgacgttc atgataatca ccgcttcttt gcgcttcggt
    26281 ttatccataa ctggagccgg ttccgctaca ggctcaggct gtggtgccgc tacgggttct
    26341 gcaggctgga aagcctgttg tgccggttgc ggtgctgaat gcacaggctg cggcgctggc
    26401 gcgacctgtg gcgaaactgg ctgctgcaac ggctgttcag gctgcggctg ataggcaggc
    26461 tgctgcaccg gctgcgctgg acgcggagca tgttgcggcg gtacctgcgc ttcaggcggc
    26521 tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg gtaggtactg gtgttgcggc
    26581 gacggacgag cagcctcatg ctcctgagcg ttagccgggg catgattcac gcggtgaaca
    26641 cgaacctcac caacgccctc atcatcttcg acatcctcgt cataagaatc gtcgtcacgt
    26701 tttgacttca ttcgttttaa tggccgatcg cggaacatag aagatcgttc tttacggctg
    26761 gtccagaaac catgtaccag taaagcgatt atggcgatcg cgccaacaat gattaatatc
    26821 agacgcaaat cctgcatcat tatattctct gttgttctaa caccttgcca ccacggcaaa
    26881 catttactca ctaagagtat ttgccgatta cctcaagtgc aagtgcacta ttaactttca
    26941 cagcacaaag atagatgaaa tcgtgctttt tgctgttttt tcgaacatat cctaactgtc
    27001 cattgcgcaa ttacccggta aaatacgcag aattttcctg ggattggtca aaaggagctc
    27061 atcctgatta tggtttcatc attcacatct gccccacgca gcggttttta ctattttgcg
    27121 caaggctgga agctcgtctc gcaacctggg attcggcgtt tcgttatttt accgctgctg
    27181 gtcaatattt tgttgatggg gggcgcattc tggtggctct ttacacagct cgatgtctgg
    27241 atcccgactt tcatgagtta cgttccggac tggctacaat ggctgagtta tctgttgtgg
    27301 cctctggctg tcatctctgt gctgttagtg tttggctatt tcttctccac gattgctaac
    27361 tggattgccg ctccgtttaa cggtttattg gctgaacaac tggaagcacg attgactggc
    27421 gctacaccgc cagataccgg gattttcggt atcatgaaag atgtgccgcg aatcatgaaa
    27481 cgcgaatggc aaaaatttgt ctggtatctg ccgcgcgcaa ttgtattact aattctttac
    27541 ttcatccctg gtattgggca aaccgtcgcg ccggtactgt ggttcctgtt tagcgcctgg
    27601 atgttagcca tccagtattg cgattacccc ttcgataacc acaaagtgcc gtttaaagag
    27661 atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc agtttggtgc tttaaccagc
    27721 ctgtttacga tgatcccgct gcttaatctg ttcatcatgc ccgttgccgt ttgtggcgcg
    27781 acggcgatgt gggtcgattg ctatcgcgat aaacacgcga tgtggcggta acaatctacc
    27841 ggttattttg taaaccgttt gtgtgaaaca ggggtggctt atgccgcccc ttattccatc
    27901 ttgcatgtca ttatttccct tctgtatata gatatgctaa atccttactt ccgcatattc
    27961 tctgagcggg tatgctacct gttgtatccc aatttcatac agttaaggac aggccatgag
    28021 taagattttt gaagataact cgctgactat cggtcacacg ccgctggttc gcctgaatcg
    28081 catcggtaac ggacgcattc tggcgaaggt ggaatctcgt aaccccagct tcagcgttaa
    28141 gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag cgcggcgtgc tgaaaccagg
    28201 cgttgaactg gttgaaccga ccagcggtaa taccgggatt gcactggcct atgtagctgc
    28261 cgctcgcggt tacaaactca ccctgaccat gccagaaacc atgagtattg aacgccgcaa
    28321 gctgctgaaa gcgttaggtg caaacctggt gctgacggaa ggtgctaaag gcatgaaagg
    28381 cgcaatccaa aaagcagaag aaattgtcgc cagcaatcca gagaaatacc tgctgctgca
    28441 acaattcagc aatccggcaa accctgaaat tcacgaaaaa accaccggtc cggagatctg
    28501 ggaagatacc gacggtcagg ttgatgtatt tattgctggc gttgggactg gcggtacgct
    28561 gactggcgtc agccgctaca ttaaaggcac caaaggcaag accgatctta tctctgtcgc
    28621 cgttgagcca accgattctc cagttatcgc ccaggtgctg gcaggtgaag agattaaacc
    28681 tggcccgcat aaaattcagg gtattggcgc tggttttatc ccggctaacc tcgatctcaa
    28741 gctggtcgat aaagtcattg gcatcaccaa tgaagaagcc atttctaccg cccgtcgtct
    28801 aatggaagaa gaaggtattc ttgcaggtat ctcttctgga gcagctgttg ccgcagcgtt
    28861 gaaactacaa gaagatgaaa gctttaccaa caaggatatt gtggttattc taccatcatc
    28921 gggtgagcgt tatttaagca ccgcattgtt tgccgatctc ttcactgaga aagaattgca
    28981 acagtaatgc cagcttgtta aaaatgcgta aaaaagcacc tttttaggtg cttttttgtg
    29041 gcctgcttca aactttcgcc cctcctggca ttgattcagc ctgtcggaac tggtatttaa
    29101 ccagactaat tattttgatg cgcgaaatta atcgttacag gaaaagccaa agctgaatcg
    29161 attttatgat ttggttcaat tcttccttta gcggcataat gtttaatgac gtacgaaacg
    29221 tcagcggtca acacccgcca gcaatggact gtattgccct cttcgtgcgt cgcgtctgtt
    29281 aaaaactggc gctaacaata caggctaaag tcgaaccgcc aggctagact ttagttccac
    29341 aacactaaac ctataagttg gggaaataca atgttccagc aagaagttac cattaccgct
    29401 ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa gggcttcact
    29461 tctgaaatta ctgtgacttc caacggcaaa agcgccagcg cgaaaagcct gtttaaactg
    29521 cagactctgg gcctgactca aggtaccgtt gtgactatct ccgcagaagg cgaagacgag
    29581 cagaaagcgg ttgaacatct ggttaaactg atggcggaac tcgagtaatt tcccgggttc
    29641 ttttaaaaat cagtcacaag taaggtaggg ttatgatttc aggcatttta gcatccccgg
    29701 gtatcgcttt cggtaaagct ctgcttctga aagaagacga aattgtcatt gaccggaaaa
    29761 aaatttctgc cgaccaggtt gatcaggaag ttgaacgttt tctgagcggt cgtgccaagg
    29821 catcagccca gctggaaacg atcaaaacga aagctggtga aacgttcggt gaagaaaaag
    29881 aagccatctt tgaagggcat attatgctgc tcgaagatga ggagctggag caggaaatca
    29941 tagccctgat taaagataaa cacatgacag ctgacgcagc tgctcatgaa gttatcgaag
    30001 gtcaggcttc tgccctggaa gagctggatg atgaatacct gaaagaacgt gcggctgacg
    30061 tacgtgatat cggtaagcgc ctgctgcgca acatcctggg cctgaagatt atcgacctga
    30121 gcgccattca ggatgaagtc attctggttg ccgctgacct gacgccgtcc gaaaccgcac
    30181 agctgaacct gaagaaggtg ctgggtttca tcaccgacgc gggtggccgt acttcccaca
    30241 cctctatcat ggcgcgttct ctggaactgc ctgctatcgt gggtaccggt agcgtcacct
    30301 ctcaggtgaa aaatgacgac tatctgattc tggatgccgt aaataatcag gtttacgtca
    30361 atccaaccaa cgaagttatt gataaaatgc gcgctgttca ggagcaagtg gcttctgaaa
    30421 aagcagagct tgctaaactg aaagatctgc cagctattac gctggacggt caccaggtag
    30481 aagtatgcgc taacattggt acggttcgtg acgttgaagg tgcagagcgt aacggcgctg
    30541 aaggcgttgg tctgtatcgt actgagttcc tgttcatgga ccgcgacgcg ctgcccactg
    30601 aagaagaaca gtttgctgct tacaaagcag tggctgaagc gtgtggctcg caggcggtta
    30661 tcgttcgtac catggacatc ggcggcgaca aagagctgcc atacatgaac ttcccgaaag
    30721 aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc gatggatcgt aaagagatcc
    30781 tgcgcgatca gctccgcgct atcctgcgtg cctcggcttt cggtaaattg cgcattatgt
    30841 tcccgatgat catctctgtt gaagaagtgc gtgcactgcg caaagagatc gaaatctaca
    30901 aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc aattgaaatc ggcgtaatgg
    30961 tggaaacacc ggctgccgca acaattgcac gtcatttagc caaagaagtt gatttcttta
    31021 gtatcggcac caatgattta acgcagtaca ctctggcagt tgaccgtggt aatgatatga
    31081 tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa cttgatcaag caagttattg
    31141 atgcttctca tgctgaaggc aaatggactg gcatgtgtgg tgagcttgct ggcgatgaac
    31201 gtgctacact tctgttgctg gggatgggtc tggacgaatt ctctatgagc gccatttcta
    31261 tcccgcgcat taagaagatt atccgtaaca cgaacttcga agatgcgaag gtgttagcag
    31321 agcaggctct tgctcaaccg acaacggacg agttaatgac gctggttaac aagttcattg
    31381 aagaaaaaac aatctgctaa tccacgagat gcggcccaat ttactgctta ggagaagatc
    31441 atgggtttgt tcgataaact gaaatctctg gtttccgacg acaaaaagga taccggaact
    31501 attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt gccggatgtc
    31561 gtttttgcgg aaaaaatcgt tggtgatggt attgctatca aaccaacggg taacaaaatg
    31621 gtcgcgcctg tagacggtac cattggtaaa atctttgaaa ccaatcacgc gttctctatc
    31681 gaatctgata gcggcgttga actgttcgtc cacttcggta tcgacaccgt tgaactgaaa
    31741 ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg tgaaagttgg cgatactgtc
    31801 attgaatttg atctgccgct gctggaagag aaagccaagt ctaccctgac tccggttgtt
    31861 atctccaaca tggacgaaat caaagaactg atcaaactgt ccggtagcgt aaccgtgggt
    31921 gaaaccccgg ttatccgcat caagaagtaa ttcttgccgc agtgaaaaat ggcgcccatc
    31981 ggcgccattt tttatgcttc cgccagcggc ggcaaaatca attcatcgct ctcatgctgc
    32041 tgggtgtagc gcatcacttc cagtacgcgc aaccccgcac ggtgcaccgc atcgtttaac
    32101 gccttccctt tcagcaaacc actgataagc tgagcacaaa acaggtcgcc agtccctttc
    32161 aggtcggttt ttacccgtga atgggaaatg acattcacgc tgtcggcact gaccaccacc
    32221 acctgcatct cctgattttc ttcattaccg gaggcgctgg taatcaccac ccattttaat
    32281 gtgtctgaaa gcagactttt tgcggcagca atggcactgt cgagatcgcg gcaattttta
    32341 ccggtcagga tttccagctc aaagatattg ggggtaattc cctgcgccag cggcagtaaa
    32401 tattgtcgat acgcctcggg aaggtcaggt ttgacataaa ttccgctatc aatatcgcca
    32461 atcaccggat cgaccatgat caataggtca ggatggtctt tgcgtagcgc agtcagccac
    32521 tcggcaagga ttttgatttg cgatgccgtt cccatatagc ccgtggttac agcacgaagt
    32581 tggcgcagcg catcacgctc ctgaagcgca cgcaaatagc cgctaaacca ttcgtccgga
    32641 atcgcaccac cgtagaaagt gtcataatgc ggcgtattgc tcagcaatac cgtcggcacg
    32701 gcaaagacat tcaagccgtt ctgttttata gcaggcaccg caatgctgtt gcctacgctt
    32761 ccgtacacta cctgcgactg cacggcgacg atatccgcct gcagcgccct actcttatcg
    32821 ttaaacaaca acaaactact catttaattt tttctccttg ccgatgatcc tcatcgtatt
    32881 ccaaccgaaa ctttacctga ttctggcagt caaatcggct attacaaaac aaagataagg
    32941 taattcagtg aagaaaatca tttgtctggc cattacgctg ctaatgacac tccccgctta
    33001 cgcgaagtta actgttcatg aagaagcccg catcaacgcc atgctggagg gattagcaca
    33061 gaaaaaggat ttgatatttg tgcgcaacgg tgatgaacat acctgcgatg aagcggtttc
    33121 ccacctgcgt ctgaagctcg gcaatacccg taaccgcatt gacactgccg agcagtttat
    33181 tgataaggta gcttcgtcgt catcgattac cgggaagccg tatatcgtga agatccccgg
    33241 taagagcgat gagaacgcac agcctttttt acatgcgtta attgcgcaga cggataaaac
    33301 ggtgcctgcg gaaggaaatt aatccgcttt ggtaaggcat ttacaggagg taacatgaaa
    33361 aaatgcttta tttatcacga tgaaaaatcg aataaatttt ggtggataga ttacgaaggg
    33421 gatagtttag ctgtcaacta tggcaaggta ggtagtattg gtaaattcca tacaaaagaa
    33481 ttcgataatg aagaacagtg tctgaaagaa gccagtaaat tgattgccgc aaaaatgaaa
    33541 aaaggatatc aagaaactcc agactttaac ttcatggatc tctactattt tgatgatgaa
    33601 gagattgggt tacatcttaa aacgtcacac ccaaacttcc agtgccattt tactgatcca
    33661 ctttatatgt gttgctggga tgaagaatct ccttttggta gcgatgaagg tgctgatgct
    33721 ctaaacgaac ttgaaaatag cctccgttct ggactgtgct gatttccctc aaatgttaat
    33781 tgaaactatg tggggtatga aatacatcgc tatggacagt attcttgaag aggatgtgcg
    33841 tgcgcaatta ctagccgatg aaatgagctc tatccagagc aatatgatta cctacgcgac
    33901 tgcattcggt cagatcaaag tcatgggtaa aatctcccat aaacttaaaa agatgggact
    33961 caatgcacta gcgcgtcatc agcttaccgc aaaaattctt caatggggtg acggtcagga
    34021 ctcaccaata cttcaaaaaa tgattgatga ccttactgct tttccacacg aaaattaaaa
    34081 actgcatttg tcggcagcaa caactgccga caaatcagat tattgacaaa gtgcactttg
    34141 tttatgccgg atgcggcgta aacgcctgat ccggcataaa aatattgcta attcaatata
    34201 ttgcagaaac attgtaggcc tgataagcgg agcgcatcag gcagttttgc gtttgtcatc
    34261 agtctccaat gctattaatc cttaaatccc tgccccctga ctaaaatgct cttccccaaa
    34321 cactccggtg gagaggtagc gatcgccacg atcgcagatg atcgctacca ccaccgcacc
    34381 agggttagct tttgccaccc gcagtgctcc agcaaccgcg ccgcctgagc tgacgccaca
    34441 gaatattcct tcccgcaccg ccagttcgcg catggtgttt tccgcatcgc gctgatgaat
    34501 atccagcacc tcatccacca gcgcagcgtt gaaaatcccc ggcagatatt ccgcaggcca
    34561 gcggcgaatg cccggaatac tgctgccttc ttccggttgc aggccaacaa tggtcaccgg
    34621 tttggattgt tcgcgcataa agcatgaaac gccggtgata gtgccggtcg tccccatgct
    34681 ggagacaaaa tgagtgatgc gcccgccggt ttgctgccag atttccggcc cggtggtggt
    34741 gtaatgcgca taagggttat cgggattatt gaactgatcg agcagctttc cttcgccacg
    34801 attcgccatc tccagcgcca gatcgcgcgc accttccatg ccctgctctt tggtgacaag
    34861 aatcagttcc gcaccataag cacgcatcgc cgcacggcgt tcctggctca tgttgtcggg
    34921 catcagcaat ttcatgcgat agcctttcag cgcggcaatc attgccagcg caatgccggt
    34981 gttaccactg gtggcttcga ttaagacatc acccggttta atttccccgc gcttttccgc
    35041 ctcgacgatc atcgaaagtg ccgcacgatc tttcaccgaa cctgccgggt tattgccttc
    35101 cagttttaac cacacttcac tgccgttatc cggccccatt cgctgcaact tcaccagagg
    35161 cgtattgcct attgtttgtt ctaatgtact cacaatctct atccatacgt ggtgttgcct
    35221 gatgcgacgc ttgcgcgtct tatcaggcct acaggttaca aaccttgcca ttaaaaagcc
    35281 cggatcgcgg gaagcgcctc cgggcgttta acattcactc aacctatcag gcgctttgtg
    35341 cgagagcaag ttcctcatcg cgggtttcaa tacgctcgtc gccgttatac agccgcgcat
    35401 gttgcagacc aacgaataaa cgctcgccat gctgcggggc atcgtcgcca tgcatcacga
    35461 ccgtcagcgg ttcgttgtac caccccagcg gctgcaccac taattgggtg tagtgacctt
    35521 tcgggctggc ttccagtacc tgtaccggca gcggcgaatc gagactggta cggcggctga
    35581 tatccacttc ccacgggcgc aggaagagat ccaccggccc ctgatacgca ggtgtgtagc
    35641 ccagcggcca gcgatgcgcg ccaacatgga actgcccgcc gcgaatggtt ccctgcaggc
    35701 ggttcacttc gcccataaat tcgagtacaa aacgggtcgc tggttcgcgc catacctgat
    35761 tcggcgcatc agcctgttca atattcccct gactcatcac cactacacga tcggctactt
    35821 cggtcgcctc ttcctggtcg tgggtcacaa acacgctggt gaatttcagc tcttcatgca
    35881 gttgacgcag ccagcgacgc agctctttac gcacctgcgc atccagcgcg ccgaacggtt
    35941 catcaagcag cagaatttgc ggttctacag ccagcgcgcg cgccagcgcc acacgctgtt
    36001 tctggccgcc ggaaagctgc gccggataac gatccgccag atgcgcaagc tggaccattt
    36061 ccagcaattt tgtcactttc gctttgatgg ctgcggcatt cgggcgctcg cgacgcggca
    36121 gcaccgtcag gccaaaagtg atattgtcga ataccgtcat atggcggaac agcgcgtaat
    36181 gctggaacac gaaaccgact ttacgatcgc gcgcgtgcag gcgactcacg tcggtgccgt
    36241 ggaagcgaat atgcccgctg gtttgatgct ccagcccggc gataatccgc agcagcgtgg
    36301 ttttcccgga accggacggc cccagcaacg cgaccatctg accagaagga atatccagtg
    36361 agatatcgtt cagcacctgg gtgcgaccaa aagacttctt gatattggca atctcaatgc
    36421 tcatgatgct cctcctgctg cgcgcgtttt tcctgattct ccaggcgcca ctgcaacata
    36481 ctctttaaaa acagggtgat aatcgccatc aacgtcaaca gcgcggcggc ggtaaaggag
    36541 ccgacggtgt tgtagtcctg ctccaacaat tcaatctgta acggcagcga aagtgtttcg
    36601 ccgcgaatcg agccggaaac caccgacact gcaccaaact cgccaatcgc gcgggcgttg
    36661 gtcagcacca cgccgtaaag cagcgcccag cgaatattcg gcaatgtgac gcgacggaac
    36721 atctgccagc cggacgcgcc aagcaaaatc gccgcttcgt cttcctggct gccctggctt
    36781 aacatcaccg gcaccagctc gcgcaccaca aacggacacg tcacaaagat ggtgaccagc
    36841 accattccgg gccaggagaa cataatttgc agattatgtt catccagcca gccgccgagc
    36901 gggccgttgg agccgtagaa cagcagatac accagaccgg caaccaccgg cgatacagca
    36961 aacggaatgt ccagtagcgt cagcagtaac tggcgtcccg ggaagttaaa gcgcgtcacc
    37021 agccaggcca gcagaatgcc gaacaccagg tttaccggta cggcaatcag cgcgatcatc
    37081 accgtcagcc agatggcgtg cagcatgtcc ggatcggcca gattctgtaa aaccggcatc
    37141 agccccttgc tgaatgcctg cacgaagatg taaatcatcg gcaccagcag gatgaacgcc
    37201 gaaaccagca tcccgatgcc aatcagaaac catttgcccc agttaatcgg gcgcgcgtca
    37261 taacgcttca attgggtaac ttccgccatt aatgacctac cacacgccga ccaaagcgac
    37321 tttgcagagt gttaattgag aacagcagca gcagagatgc cgcgaggatc accgaagcaa
    37381 tcgcgctcgc tgccgggtaa tcaaactcct gtaagcgcac aaaaatcatc agcgacgtca
    37441 cttccgtctt ccacgcgata tttccggcga taaaaatcac cgcgccaaat tcaccaagac
    37501 tacgggtaaa cgacagcgcc acgcccgcca ccagcgccgg agaaagctcc ggcagcacca
    37561 ctttgcagaa actctgccag cgcgttgcac caagcgtttc cgccgcttct tcatattccg
    37621 ggcctaactc ttccagcacc ggctgcacgg tacgcaccac aaacggaatg ctggtaaagg
    37681 ccatcgccac cgcaatacca agccaggtat aggtgacttt gatatcaaac ttcgccagcc
    37741 attcaccgta aaaaccgttt acggaaaaga gcgaggccag cgttaaaccg gcgactgccg
    37801 ttggcagcgc aaagggtaaa tccatcagcg catcaagcag cgtgcggccc ggaaagcgat
    37861 aacgtgttaa aatccacgcc atcagcagac cgaaaacgcc gttaaaaatc gatgccacaa
    37921 acgccgacag cagcgtcact ttgtaggccg cgaccacctg cgggttggtg atcacctccc
    37981 agtactgcgc ccagctcatc tcagaaagct gcatcaccag cgcagagagc ggcagcagca
    38041 atatcaggca cacaaacagc agactggtgc caaggcttaa agtaaagccc ggcagcacgc
    38101 gtcttgaaga aactgcaaac attacttacg ccccgccgct aacagcttgt ctaactcgcc
    38161 gccgctggtg aagtgggttt tcatcacttc cggccatgag ccaaatttgt cttccacgcg
    38221 gaacagttcg gtctgcggga atttatcttt cagtttgtcc atgacttccg ggttattcac
    38281 acggtagtaa tagtcggtga tgatggtctg cgcctgtggg ctgtagagcc agttcaggta
    38341 ggcttttgcg gctttttccg taccgttggc ctgcacattt ttatcgaccc acgccaccgg
    38401 gaattccgcc agaatattgg ttttcgggat caccacttca aagccctgtg cttcatactg
    38461 tttacggatg ttgttcactt ccgattcaaa gctaatcagc acatcgccca agccacgctc
    38521 ggcaaaagtg gtggttgcgc cacgaccgcc ggtatcgaac acttcaacgt ttttcaggaa
    38581 ctgggtcata aactgttcgg ttttggcttt atcgccaccg tcagctttat ccgctgcgcc
    38641 ccatgccgcc agataggtat aacgcgcgtt acccgacgtt ttcgggttcg ggaaaatcag
    38701 cttcacgtcg gagcgcacca ggtcgttcca gtcgtggata ttcttcgggt tgcccttacg
    38761 caccaggaag cccatggtgg agtagaacgg cgagctatta ttcggcaggc gcgactgcca
    38821 gtcagccggg atcagcttgc ctttgtcgtg caggatttgt acgtcggtca cctggttata
    38881 agtgacaacg tctgctttta agccctgcaa aatcgccagc gcctgttttg atgacccggc
    38941 atgagattgt tttatcgtca gtttgtcgcc gccgttatct tttgcccatt gctgttcaaa
    39001 cggcggattc agggcggcaa acagctcgcg ggagacgtca taagaactgt tcagcagttc
    39061 cgttgcctgt acatggcccg ccagcagcag agaagcgacc agcgcgagtg agttcttttt
    39121 cagtaagtta acggccattg cgcaccctta taaatttaat gactttctaa tagccatcat
    39181 atttataacg gacgtgaaag gagtaacggt tttatatacc gtttggtgat ttggaagtta
    39241 aaaagggaat aagaacttgg ttcaacaggc gtaattgttg caggcagttt ggacacggac
    39301 agcgcggaga aaccggagcg tacacatagt acgtgaggat ttcgagcact gcccggggcc
    39361 aaaatgacaa atgaaatagc ctggtgaact tagttcaaga cgaaatcctc cccacacatg
    39421 cagggaggaa acagaggtga atcagatacc gacgctaacc gtctccggca gtgtgctgcc
    39481 gccatcaatc acattctgtg taccggttaa atagctggat tcatccgatg cgaggaaggc
    39541 cgccagttcg ccgacttcca gcggatcggc gaggcgacac agcgggattg cttttgccat
    39601 ttcagtcagc accgattctg gatcttccgg gttcgactgg cgggcaatgc tttccgccat
    39661 tggcgtgcgg acgtatcccg ggcagatggc gttaacgcga ataccggatt gcgcgtactc
    39721 caccgccagc gatttagtca ggccaacaat cgccgctttc gttaaggcat acgccgtttc
    39781 gccaggatcg gccaccatat caccagtgac tgaagacatc atcacaatgc gaccatcttt
    39841 gcgcgcaatc atctccggca gcaccgcctt cgtgacgttc catacgcctt taatattgat
    39901 atcaatatgg aaatcgcgat cttcatcgct catatcgagg aaactgccca gacgacaaac
    39961 gcctgcgtta ttcaccagga tatcaatgcg cccttctttt tccttcgcgc gtttgatagc
    40021 tgcggctacc gacgccgggt cacgcacatc ggcgacaacc gccgtacagc gatgaccacg
    40081 accacacagt tcgtccgcta gcttttcgat ctcaggggag atatccagca agattaggtt
    40141 cgcgccatga cgtgcaaaag ttctggcaat tccttcgcca attccctgca atgcgcccgt
    40201 aatcagtgct gtcttgcccg tgagtttacc cattttaatg ctctccttgt taatacagag
    40261 cgttacactc cattccctct gaaaatacag gataaacaaa atattgctgc ggatcagctc
    40321 actaattcag gaaaatctca atccagtcgc tgctttatgc atcatttcag gcgttgtgtt
    40381 agctcactgc tgcgctgaat cattttcaat gacgccacat cattgatgtt gcacaagacc
    40441 aacaaacagt aaatcggtaa cagaattttg cgccgtgcgg gtagacatcg acgagctacg
    40501 ccattcggtt tcaccggaaa cggtatcgag cgtgaaatgc gccagccgcc gcagcggaga
    40561 gtcagccaga gaagtaatgg caatcaccgt tgccccctgc tttcgtgccg cttctgcgca
    40621 caagacaatc tcttttttac tgccgctgta agagatagcg atttgtacat cgcctttttt
    40681 caacgcctgg gaaacggtgg cttgtacgtg cgtgtcggct tcgcaggcca cgcgatagcc
    40741 aattttcatc agtttgaacg acagatcgcg ccccaccagc gccgatccgc ccaggcccgt
    40801 tatctggata aacggtgctt tactgatgac ctcaatgatt ttttgcagcc gcgcgtagtc
    40861 gaacaacgca caggtttgct ccagcgccag ctctttttcg cgattcagtt tgcgggcaat
    40921 cacttccagc gaatcatcac tggtaatcga actgtgcagg tgcagcgccg tggcattcgt
    40981 tttctcccgg ctggcactgt attcgccaat taacgccata cgcaattcag taaatccttg
    41041 cgcgccgagc ttctgggcaa atttcacaat gctcgactgg ctgataccga gctgtttcgc
    41101 catctgccgt gaagagacgg atttcagctc gctcactctg gcccgcagaa agtcagcaat
    41161 tttttgctcg ttcccggtaa attcactttc cgcattgcgt attttcgtca ggtacaacat
    41221 cttccactct cctgggctgg cagcaaaaat tccgcttaag ttcttatgac gctcttcaca
    41281 ctctgcgagt gtagactcaa tgattccttt aagtctgtca attcgggata tatgattcca
    41341 tacatctgca attatttgat cgtaaatagt aaggtcacca ccgatgcaac tagaaaagat
    41401 gattactgaa ggctcgaaca ccgcctcggc tgaaattgac cgcgtatcga cgctggaaat
    41461 gtgccggatt atcaacgatg aagataaaac cgtaccgcct gccgttgatc gcgtcctgcc
    41521 ggatatcgcc gcggcgatcg atgttatcca cgcccaggtc agcggcggcg gtcgtctgat
    41581 ttatctcggt gcgggaacat ccggtcgtct ggggattctg gatgccagcg aatgtccgcc
    41641 cacctacggc gtgaaaccgg gtctggtggt tggtttgatt gctggaggcg aatatgccat
    41701 tcagcacgcg gtggaaggag ctgaagatag ccgggaaggt ggtgttaacg atctgaaaaa
    41761 tattaattta acggcacagg atgtggtggt tggcattgct gccagcggtc gcacgccgta
    41821 tgtgattgcc ggactggaat acgcacgcca gctcggctgc cgcacagtgg gaatttcctg
    41881 taatccgggg agcgccgttt caaccaccgc tgagtttgcc attacaccga ttgtaggtgc
    41941 cgaagttgtt accggttctt cgcggatgaa agcaggtaca gcgcagaaac tggtgctcaa
    42001 tatgctttcc accgggctga tgattaaatc cggcaaagtg ttcggcaacc tgatggtcga
    42061 tgtggtcgcc accaacgaaa aactgcatgt gcgccaggtc aatatcgtta aaaacgccac
    42121 cggatgtaat gccgaacaag cggaagcggc gttaattgcc tgcgagcgca actgtaaaac
    42181 ggccattgtg atggtgctga aaaatctcga tgccgcagaa gctaaaaaac gcctggatca
    42241 acacggcggc tttattcgtc aggttttaga caaggaataa cccatggcca aagagatcag
    42301 cagtgaactt ctgaacacca ttcttacccg tgtcggcgga ccgggaaata tcgccagttg
    42361 tggtaactgt atgacgcgcc tgcgtctggg tgtacatgac agttcactgg ttgatcccaa
    42421 tatcaaaacg ctggaaggcg tgaagggcgt cattttgacc agcgaccagg tgcaggtcgt
    42481 ttttgggcct ggtaaagcgc atcgtgccgc aaaagcgatg agcgagctgc tgggcgatgc
    42541 accggtacag gatgccgcag aaatcgccgc ccagaacaaa cgtcagttaa aagccaaaca
    42601 aacctccggc gtgcaacaat ttctcgccaa attcgccacc atcttcacgc cgctgatccc
    42661 cggttttatt gccgccggtc tgctgctggg gatagcgacg ttaattgcca cggtgatgca
    42721 cgttccggca gatgctcagg gcacgctacc cgacgcgctg aattttatga aggtgttcag
    42781 caaaggtttg ttcaccttcc tagtgattct ggtgggctat aacgccgccc aggcatttgg
    42841 cggcacgggc gtcaatggcg caattattgc cgcactcttt ttgctcggtt ataaccccgc
    42901 tgcaaccacc ggttactacg ccggttttca cgatttcttt ggtctgccca tcgatccgcg
    42961 cggcaatatt atcggcgtgc tgattgccgc gtgggcctgc gcccgcattg aaggcatggt
    43021 gcgccgcttt atgccggacg atctcgacat gctgctgacc tcgttaatca ccctgctgat
    43081 caccgctacg cttgcgtacc tgatcattat gccgctgggt ggctggttat tcgaaggtat
    43141 gtcatggctg tttatgcacc tgaacagtaa tccgttcggt tgtgcggttt tagccgggct
    43201 gttcctgatc gccgtggtat ttggcgtgca tcagggcttt attcctgttt acctcgcgtt
    43261 aatggacagc cagggattca acagcttatt tcccatcctt tcaatggcag gcgcgggcca
    43321 ggtgggcgcg gcgctggcac tctactggcg ggcgcaaccg cacagtgcgc tacgcagtca
    43381 ggtacgcggg gcgattattc ccggcctgct gggcgttggt gaaccgctga tttacggtgt
    43441 caccctgccc cgcatgaagc cgtttgttac cgcctgttta ggcggcgcgg cgggcggttt
    43501 gtttatcgga ttgatagcct ggtggggtct gccgatgggc ttaaacagcg cctttgggcc
    43561 gtctggtctg gtggcgctgc cgctgatgac ttccgcacaa ggcatccttc cggcaatggc
    43621 ggtttatgct ggcgggattc tggtggcatg ggtttgcggg tttattttca ccacgctctt
    43681 tggctgccgt aacgtcaatc tggactgata ttatgaaacg gacaatgctc tatctttctc
    43741 tgctggctgt tagttgtagc gtcagcgccg caaaataccc tgttctgaca gaaggtagtt
    43801 gatcaagtat atgcggcgtt aaagcagtaa taaaaaggcc agtcagtaat gttactgcct
    43861 ggcctttaat caatgaatca gaaacgctta cagcgccatc aacttgtcca gcgacggtgc
    43921 gaaataatag ccgccggtta ccggtttggt gaaacgcaac atcgcatcac gtttgccatc
    43981 ggtatcgcca aacatgctca gcagttgctg ctcaatgtta tgcagacgcg cgcagtaggc
    44041 gcagaagtac agaccgtgag tgccactggc ggtgccgtac ggcaggctct ggcgaacaat
    44101 cttcagccct ttgccatctt ctttcagatc aacgcgggtg aggtgagagg tttccggacg
    44161 ttcgtcgccg tcaatctctt cgttggcctc tttggtgcgc ccgatcatca tctcctgatc
    44221 gtgaacgctc atccggttga gctgcttcag gttgtgctcc caacgctgca caaacacata
    44281 gctgccgccg gaatccacgc cgtctttgat aaccgccact tcgcggcgcg tctcttcacc
    44341 cgccgggttt tctgtaccgt caacaaagcc gctcaggtca cgctcttcca cccaacggaa
    44401 gccgtggatc tcttctttca cttcaatgca gtcaccaaag gcttccatcg ccgcctgagc
    44461 gacagagaag tttacgtcgt gacgcagaga aagaatgtgg atcaacacat cgaactgggt
    44521 cgtcggcgca aggcctttac cgtagcccgg aaaatctttc agctcttctg ccccaacgcc
    44581 gccgctcaga gcgcgccagg tgttgttacc aaaggcaacc accgcaccaa gatgcgcgtc
    44641 cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat gttttactgg ccgcacgcag
    44701 ggcgtcaact tcccctttca cgttggcttc gatccaaatc gccgcgcggc aatgttctgg
    44761 caaaatgcca ctctgaacct gagacattgt tcctcctgaa aataagaatg ccacgcaatc
    44821 cgtggcattg ttgagcgcta ttttacgtgt tttttcgcga cggcggtttg ttcagacgca
    44881 aattaacgcc gccagataat tttactgact ttccagtttt tcagggtatc gtccgaaggc
    44941 attaaccctt caggaccacg ccattccccg ctgaactggt agctaatatg ttgactgcct
    45001 tcggctttac attcgacggc acgattatca tcaccatcag ctttttggca attgccaaat
    45061 gctttgctgt aaaggtcgct aaacggtgta ccgattttaa caccggtgtc agcaggaata
    45121 tcgctatcca gcacgtcaat gcgactgatc gtgccttgat cgccgttaat caccatcgcc
    45181 acgttgtcgc ctttcatcac ttcaaaaaag cgcaccacgt tgccgttcgt ggttttcatt
    45241 ccgctgcgca ggcgataatc gccatcaagc gcatcagcaa tggcttgttc ttgcagtggt
    45301 gtggacgccg ttaattcacc cacgccctgc tcgctcactt tggtggacga tccaaaccag
    45361 ttccacgggt tagcggcaga ccagttaact gacgacatcg tggaacagcc ggtcagcatc
    45421 aacggcatag cgcataacat taaacgcagc gatttcatgt cacttccttt ggttattcaa
    45481 taacgttgct tggagtgcaa attcaccaaa aagtgccgtt attctttttc ttcattaaaa
    45541 caagcgcgta aacgtcgatt ggtcagcagc cagattaacg ccacaatatc cgccactacc
    45601 agcgccagac cgataccgct aacggattca ccgttcagcc acagccacgg ttgccagcaa
    45661 agtaaaacca cctgcgccaa cagcaacaga aaatagagca catgccaggt gcgaggaaac
    45721 gaagcccgcc gaccgctcaa caaaaacgcc agcaccgccg gaataccggg aatcagcccc
    45781 agccagaaat tatcgtgatc gggataaaac agatttagca gcgcagtacc ctgctcacgc
    45841 gacgcaccgg caatgacaaa cagcacccag gttcgcgcct gaagcaatag cacaagccag
    45901 aagagcaagg gtaaacgcag gcgtccgtgc gcatcataat ggacaggatg gaactcagta
    45961 ctcttcatct tcaatcaaac gcttacccag actcagcacg tcggcgtgtt catatcccag
    46021 gcgttcatac attccgagca ccatgtcgtt atcttccggc acgttgatct gaattttcgg
    46081 gcagccgcga gcaatcagct ttttctccag ccggttaagc aacgcattgg caattccacg
    46141 cccacgaaac tctggatgca cgccaagata ataagcagac ccgcgatgcc cgtcataacc
    46201 gcccatcacc gttccgacca cttcaccgtt tacctcagcg accaaaaaca aactgacgtc
    46261 atggttcatc ttacgctcga tgtccatttc cggatcgttc cacggacgca gcaagtcgca
    46321 acgctcccaa agggtgatga cctcttcgaa atcttcctgg cgaaatacgc gtatctccat
    46381 ggtattcgtt acctttttgc gggttaaaag gctgattatg gcgtgaacgg tcgaattagc
    46441 caatatctga cgaaaatcgg ttgaaaaagt ggcataatgg ggagttgtca actattgaaa
    46501 tgaaaagtaa aacaattctc aacagcaaac cgtcgtaacg gattacgcga tacgatataa
    46561 catctggaac tttattatta caactcaggc cgtatgagca cttttaaacc actaaaaaca
    46621 ctcacttcgc gccgccaggt gctgaaagcc ggtttagctg ccctgacgtt gtcaggaatg
    46681 tcgcaagcca ttgccaaaga cgaaccttta aaaaccagca acggacacag caagccgaaa
    46741 gccaaaaaat ctggcggcaa acgtgtcgtt gttctcgatc caggccacgg cggaattgat
    46801 accggagcga tcggacgcaa cggttcgaaa gaaaaacatg tggtgctggc gattgctaaa
    46861 aacgtccgtt ccattttgcg taatcatggg attgatgcgc gtttaacgcg ttctggcgat
    46921 acgtttatcc cactttacga tcgcgttgaa atcgcccata aacatggcgc agatctgttt
    46981 atgtcaattc atgccgatgg ctttaccaac ccgaaagctg ccggtgcttc ggtatttgcc
    47041 ctctctaacc gtggggcaag tagcgcaatg gcgaaatacc tgtctgaacg cgaaaaccgc
    47101 gccgatgaag ttgccggtaa aaaggcgact gacaaggatc acctattgca gcaagtactg
    47161 tttgatctgg tgcaaaccga tactattaaa aacagcctga cgcttggctc gcatattctg
    47221 aaaaagatta agccggtgca taaactgcac agccgcaaca ccgaacaagc cgcgtttgtg
    47281 gtgttgaaat cgccgtcggt tccttcggtg ctggtggaaa cctcgtttat caccaacccg
    47341 gaagaagagc ggctgttagg cacggcggcg tttcgtcaga aaatcgccac agcgattgct
    47401 gaaggcgtga tcagttattt ccactggttc gacaaccaga aagcacattc gaaaaagcga
    47461 taagttatga aacccgacgc acaccaggtt aaacagtttc tgctcaacct tcaggatacg
    47521 atttgtcagc agctgaccgc cgtcgatggc gcagaatttg tcgaagatag ttggcagcgc
    47581 gaagctggcg gcggcgggcg cagtcgggtg ttgcgtaatg gtggtgtttt cgaacaggcg
    47641 ggcgtcaact tttctcacgt ccacggtgag gcgatgcctg cttccgccac cgctcatcgc
    47701 ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt cactggtagt gcatccgcat
    47761 aacccgtatg ttcccaccag ccacgcgaat gtgcgttttt ttattgccga aaaaccgggt
    47821 gcagagcccg tttggtggtt tggcggcggc ttcgatttaa cccctttcta tggttttgaa
    47881 gaagacgcca ttcactggca ccgcaccgcc cgtgacctgt gccagccatt tggtgaagac
    47941 gtttatcccc gttacaaaaa gtggtgcgac gattacttct acctcaaaca tcgcaacgaa
    48001 cagcgcggca ttggcgggct gttctttgat gatctgaaca cgccagattt cgaccactgt
    48061 tttgccttta tgcaggcggt aggcaaaggc tacaccgacg cttatttacc aattgtagag
    48121 cgacgtaaag cgatggccta cggcgagcgc gagcgcaatt ttcagctcta ccgtcgcggt
    48181 cgttatgtcg agttcaatct ggtctgggat cgcggcacgc tgtttggcct gcaaactggc
    48241 gggcgcaccg agtcgatcct gatgtcaatg ccgccactgg tacgctggga gtatgattat
    48301 cagccaaaag atggcagccc agaagcggcg ttaagtgagt ttattaaggt cagggattgg
    48361 gtgtaactcc ctcaccccca ttcccgcatc cgctgatgca acgtcaacga cggcttctcg
    48421 gcaaacagct gctggtaatc cgtggcaaat tgccccagat gccagaatcc ccactgcatg
    48481 gcggcgtctt ttaccgttgt gctttgcgac cacgggctta tcagttcgcg gcgtacgacg
    48541 ttcaggcgaa tgcgtttcag ccacgcgttt gggccaatgc ctaaaatagc gtgaaacgcg
    48601 ttttgtagcg tgcggcgact gacatgcagt tgattacaca aatccagcac cgtcaccggt
    48661 tcggacatgt tttccagcac atattcacgg gcgcgggaaa gcaaccgacg gtaactctga
    48721 tgactgatgc tttccgccgt caccattggt tgcgcttctt ccagcatcgc ccccatcgcc
    48781 attagcaaat tatcccccag cacttttcgc actgcaggct gatggagatt ttccgggttc
    48841 tcgcaaaacg tcgccagcgc ctgttggaca aagccccaca gcgcggcttt atgctgctct
    48901 ttcacttcca gcgccgactg gctacgcagc atatgtaata cccgatccgg attgtgcaaa
    48961 aagttagcct gacaggtgat gacatcttca gacagcacta cgcccaggat cgtataatcg
    49021 tccggcgtac ttaattcaaa ctcggttcca ccggggcgca tcgctatttc tgcgcttccc
    49081 agacattgcg aaccgataaa tccctgctca ccgcgcgtcg ccggaatgcc aaaccagaac
    49141 gagttcggcc agaccaggca cgactgacgc agcgccagac cggtgtattc acgaaaaacc
    49201 tgaatatcat cgagtagaat ttccgtaaat tcaccatgaa acttgcccgg atgcagctga
    49261 tcgtaaatct gctgctaggc ggtaatcgtc aaagcatgtt catagacatc cgttgtctgt
    49321 cgttgatgaa cattatccac ttcgaccttc ggcgtgagct tcaggttttc gggtaagggt
    49381 tcatgataaa gatggtgcaa attggctgta cgggtctttt tcatgatgtt aatgccgggt
    49441 gttgtaggac acccggcacc tccgacaggt taatggggct tgagacgata acgactactg
    49501 cgtttacgta acgtcccggc agaaaagagc tgttcgagcg catttctggc tttttccagc
    49561 ggccagccaa agtgggcggc cacttctcct gccgtcattc cctgacatac tgatgttaac
    49621 agcgccaaca actcgtcagc agattccgcg acgataactg gcgcgtcggg ttcctttacg
    49681 ggttgcttcg gctggcggtt aaagccagta accagccact gggtgtcatc gtctggccga
    49741 ccgatctcct tacggctgat gacacgcccg gtacgcatcg cggcagcaca acctgcgtcc
    49801 agcgccgcac gacacgccgc cagatcgcct tccaccacca gcgttaagcg accagggtca
    49861 agcacttcgt gactgagcag acgaacgtta gctgctttga gcatggcatc cgctgcatct
    49921 atcgcggcga ccatgccgtc cacttccagc aatcccagtg cattgatcat cggcaacctc
    49981 cgttacgcac gctggattgg attacgcgcg atttccagca ctgcatcggt aaaggcgtta
    50041 caggctgctt tacacgcggc ctggctaccg gttaaaaatg ctgccgagta gttggtttca
    50101 gacggtggcg ggacgtaggt caccagctgt acgtcggcag atttcaacgc ggcatcaatg
    50161 ccgtaggtcg cttccagcgg cggtgccacc agatacgcca tcggatcgcc aagcgtgatc
    50221 ccggcggttg atgaaagata agaaccggta cgcgaaacca catgcgccag gaaggcggtg
    50281 ttttctgcgt cattggccca ctggaaagct gcgccatttt caatatgtgc aaccatcgca
    50341 tccagaccgg cacgcacttc cgccgggttt ggcccaccaa gcataatcag cacttcaccg
    50401 gcagtcggtg acgggccgtg tgccgctccc gcatacagcg agcgaccata taccacttcg
    50461 accatcgctt gtttggtcgc ttcgtcagcc gcaatgtagg ttacgtcgtc agaatcggca
    50521 gaaatcagcc cgaggctacg aatatgcggc ggcaatttta gttcacgcgc aaattcagcg
    50581 ttaacggagg caatcacccg catggcggtt accgacggtc gaatcaaatc taaagctggc
    50641 atgatgcctc cttatcgggt catgttgatg ccggatgctt tctgctccag catacgtttc
    50701 gccaaatcca caatgacggc acagcagcac gaaagagaag caaaagtcag ctacctggta
    50761 cactaggcag cgggtcagca aaggggggca aatgcccctt ttttcatgtc agataatcag
    50821 atcattacct caataaacta ataatcacat cgcggttaca accagtttga tagtgatagc
    50881 tcaaacccag acgtggcgct ggctgcaagc aaaataagcc gcaaaaatgc gaccccatgt
    50941 ttactagccg caaaaatgcg gtcctatttg ccagaacctt ttattattca attggttagt
    51001 gcaagaaaaa gcgtgattac tgttcaagaa gtatccagaa aggtcatgat tttgacgctg
    51061 aaaaaaaata ggtgggcatt tttgcccact cactagccgc aaatttgccc actcactagc
    51121 cgcatttttg cggcccaaag taataaatac agagaaataa taaatacaga tcagcgccaa
    51181 gatttttcgt cttttcggcg gaatcatgag actagtgctt acgtcctgat aacctccaga
    51241 gcagacctta acttctgtgc agcaggacta cgctcattgc cgctcggcta ggtcaaatcc
    51301 tcacgataaa gctgttcgga ttgtgaccat aatgttaaag gactctttaa atcattcctt
    51361 gacgaattaa ctagctggct taagcggaat agcatgcagg ataaaaacaa aaccagctta
    51421 gcagtgggga gagcttcgct tcgctcgtct cctggggcca atgctcaatg aaacgacgta
    51481 aggcacggag cgctcaccat aggtagacac ataaacatct gagagcgcac cttctatcgc
    51541 gctacgcgct caataaaaac caacgacacc gccacactgc aagaccaatg gtttttatcg
    51601 cgtatacaga ccagacagga aacagcagca aagcagcgta tagccaggtg cagacagcac
    51661 actcacacga cgcacagcag gcttaagtga tcactcagga aactttatcc ccgccgccga
    51721 tcaccgcgaa ctatcatgac cgcgccaaat tcgaaaggaa tggcccatag aacgcgatga
    51781 acacacataa gcgctacact tacttaacgt cgccacagag cgattgagag cgcttaaaat
    51841 cgatgcgatt tcattaaatt tttctcactc gcctgaatgg tggcgatcag gactgatcgc
    51901 aacattgccc cgcctcgata ttgagcgcat aatagataca tacattaagc ggaaaagaga
    51961 taagtttatt gtgaaaatca gggggttaca tatcatgcgc atatcagaac aacaacggca
    52021 gatcctcgac tatctggcaa aaaacgaatc aatcaccgca cgacaagccg cagatctcgt
    52081 ttatggtggc aatgttaccc gcgtccaggt tgacgccgcc cgccgctcac tcatgacgat
    52141 ggttagtaat gggctaatca ggaaacaagg ccgcgctttc gtagctgaaa gacatatcaa
    52201 tcacgcttat agccaggacg ttgaagaatt gcgccgccag atcctttggg tattcagcaa
    52261 acagggtcag gagtatttcc tttctgaaca cccaaatttc gccgccaaag agttaacagt
    52321 aagcggattg tcgtgcgcac tggattatct tgtcggtgaa ggttgcgtta cttacaagca
    52381 gctatggcac gccctgaata cgcttgtaga agatggttta ctcattgcca cgcgtgagaa
    52441 catcgaatac tggataatgc cgcgttcagg tcgccgcaat catgcacggt atatttcagg
    52501 caacagatac agaacgccgg actttaatcc gacacaggaa gatcgggacc gtgccgaaat
    52561 gatgtgcagg gaacaggaga aaagaattat ggataagtta ttttcgatgc agtgaacaca
    52621 agcgggcgca atgcccgttt ttattgcctt attccacgcc tgacgaatca atttgatcac
    52681 acctgagaaa atgcatagcc agaagttaag gccgttatat tgcgattggg agcgttttaa
    52741 acggtatcaa tttaattaat tctgatgtgg tgatttgcag gcaaaaaaaa cccgccggag
    52801 aagcgggaaa tagagacata tcgttgtagt tattaacaga tcactttgag gtcgagggcg
    52861 gctaaccctc gctaatatta tcccgctatt accttgcggg tgcaaataat cttttattgc
    52921 tttgatgatt taataaatag cactttttgc taacaaaaac cgctgtcaat atatggccaa
    52981 aaatgtcaac agaaaaaata aggtttataa tattaagtat cacatgggta atgggttcag
    53041 ggaatcggaa tccaccgcct cacggaaatt caaaatttgc ttcttctttc ctccgtgggc
    53101 ggcttctttt tcgtatcgag gtatttatga aagcaataac attagaacag ttcgcagcaa
    53161 tggcgaatgt atcgaccaga acgctgaggc gctggctaaa aaaaccatca accggagtcc
    53221 ctttgccatt taccccacgc ggtactcgcc aattattctg ggagcacgaa tgcaaagaat
    53281 ggttgtacag aacagaacga taataatcac gcatcacatt ttgaggtaaa catcatgacc
    53341 aatatcgaaa gattagaacg ctgtgccgat attatgcgac gcagatggat ttatgaccct
    53401 gaagcgggtt tattgtttag ccgtgaaaca aaagacgtta tcaaaggcag catgaccaat
    53461 aaagggcatt tggttgtaac ggtacatgaa ggtaaattca aagtgacact cacctatcag
    53521 aaagcctgct atgtatacac atacggcgct tatgacgaga cattatatga agtgcaccac
    53581 gttaatttga acaaacagga taaccgcatc aagaatattc gcctgatgcc gaaagaaaag
    53641 caccgccgga tacataaaaa cgtccgcaaa cttattcggc tgggccacac acaatggctt
    53701 caaccgcaat taattgaaca atcactataa acacaggagt aaataccatg cattcattag
    53761 acagttattt tcagcgcacg acagccccta aatcagccgc ccaggaacgc cgtgaggaat
    53821 tccatgagaa agtgatgcgt tctgctgatt atattgcgga caagttcgtg gaaacggttc
    53881 gtccacttgt tgatgaagta gctgataaac ttcagtctga aatgccggaa gatatggaag
    53941 gcactgcaaa gcggcgactc atttgcgaat taagtcgtcg ttttggcgtg tcaataagcg
    54001 cttttaaata atcgaaaaaa ggggggaaag ataatggacg catttgaggt actggcaaat
    54061 aaaggcgctg aactggtagc acagcgtgat aaaaccgcca acgaagggga acgcgcactg
    54121 ctaaataaac agattaaagc gatccgtatg gcccagttta aattaatcag taatgaagtt
    54181 atcgaaacaa ttaaaccgca gatcccacaa atcgttgctg acgctattaa agccgccgga
    54241 ctggagaaac gcattgccgg gctaaaaacg aacgcagaga aaatggagat gtacaaacag
    54301 agcggtgctc cgaatcccga tgagtacttc tcaccggatg ttgagtttat ggcgcaggtt
    54361 gaggaacgtc tgggtagctg tctcaccgag gaacagcgtc gctattttga tggagtggat
    54421 agttcggcag gtattgacct gaactcgtat ttcggtcgtg aaattgagca ttttgatgcc
    54481 gccccgtcta tgccggagcc agaaccagaa atggccaccg atcctgaagc tgagcaacgg
    54541 aaacgcgtat tcaatgcgat agggattcgg tactgatcgg ataaaagaaa gccgggaatt
    54601 accccggctg acttaacaat gtgaccaatg aacaaatcgg acaagacaaa caggataagc
    54661 agtctcgaaa caatgttcgg gaactatatt agcacaagtc cggagaaaat catcatgttg
    54721 gcactacgca tcatgcaggg catcgctaag accctcgcag aacatgtctt agacctgaaa
    54781 cattcgccac tgagcaaaca ggccatgaag cgccaaacgc ttcgtctgtg ggctgaatat
    54841 tcgcttggaa ctatcaataa aatcatcgac atgaaatcgg ggccgagcaa ccagagcgcg
    54901 gaggaaatgg agttcatcag gcgtttaatt ctcattcgca gagacattca tagccagtta
    54961 cacagcgtgg gaatcgatat taatgatggg acgggcgatt aattttcgga caagtactat
    55021 gcgcgcgcga ggagctgaac atgaagcaag atattgcaga caggctggag atactcgaag
    55081 gccagcgggc agaagcgaaa caactacgta agcaggccag aagagcgcac aggaacaacg
    55141 aagcagagtt actaaccaaa tacatcagtt tcaccaatta ctgcatctac gagtgttaca
    55201 aggaagatgc agaggactgg ctggattcat tgccagaaca gtactgagca gaataacacc
    55261 acgtcatgtt gttctgaggt gagcatatca cctctgttca ggtggccaaa tttagtgtgc
    55321 cacttcagca tcaccgttga aatcacaata acgatttttt tgtaaatatg ctctcacata
    55381 tgtaggaata gaccaggttg gcattgaaac acatctacca tgaccataat aaagta
//
LOCUS       DAZXUL010000008        55421 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZXUL010000008 DAZXUL010000000
VERSION     DAZXUL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55421
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.008"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(116..1489)
                     /gene="argH"
                     /locus_tag="ACH3L1_000460"
     CDS             complement(116..1489)
                     /gene="argH"
                     /locus_tag="ACH3L1_000460"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFT4075356.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1550..2326)
                     /gene="argB"
                     /locus_tag="ACH3L1_000461"
     CDS             complement(1550..2326)
                     /gene="argB"
                     /locus_tag="ACH3L1_000461"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFT4075357.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2334..3338)
                     /gene="argC"
                     /locus_tag="ACH3L1_000462"
     CDS             complement(2334..3338)
                     /gene="argC"
                     /locus_tag="ACH3L1_000462"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFT4075358.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3492..4643
                     /gene="argE"
                     /locus_tag="ACH3L1_000463"
     CDS             3492..4643
                     /gene="argE"
                     /locus_tag="ACH3L1_000463"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFT4075359.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSCDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4995..7646
                     /gene="ppc"
                     /locus_tag="ACH3L1_000464"
     CDS             4995..7646
                     /gene="ppc"
                     /locus_tag="ACH3L1_000464"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFT4075360.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7829..9562
                     /locus_tag="ACH3L1_000465"
     CDS             7829..9562
                     /locus_tag="ACH3L1_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFT4075361.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9711..10562
                     /locus_tag="ACH3L1_000466"
     CDS             9711..10562
                     /locus_tag="ACH3L1_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4075362.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10549..10890)
                     /locus_tag="ACH3L1_000467"
     CDS             complement(10549..10890)
                     /locus_tag="ACH3L1_000467"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFT4075363.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10892..11770)
                     /locus_tag="ACH3L1_000468"
     CDS             complement(10892..11770)
                     /locus_tag="ACH3L1_000468"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFT4075364.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11736..14033)
                     /locus_tag="ACH3L1_000469"
     CDS             complement(11736..14033)
                     /locus_tag="ACH3L1_000469"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFT4075365.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14084..14404)
                     /locus_tag="ACH3L1_000470"
     CDS             complement(14084..14404)
                     /locus_tag="ACH3L1_000470"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFT4075366.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14419..15498)
                     /locus_tag="ACH3L1_000471"
     CDS             complement(14419..15498)
                     /locus_tag="ACH3L1_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFT4075367.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15807..18308
                     /gene="ptsP"
                     /locus_tag="ACH3L1_000472"
                     /pseudo
     CDS             15807..18308
                     /gene="ptsP"
                     /locus_tag="ACH3L1_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            18320..18982
                     /gene="fsa"
                     /locus_tag="ACH3L1_000473"
                     /pseudo
     CDS             18320..18982
                     /gene="fsa"
                     /locus_tag="ACH3L1_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18993..20094
                     /gene="gldA"
                     /locus_tag="ACH3L1_000474"
                     /pseudo
     CDS             18993..20094
                     /gene="gldA"
                     /locus_tag="ACH3L1_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20369..20976
                     /locus_tag="ACH3L1_000475"
                     /pseudo
     CDS             20369..20976
                     /locus_tag="ACH3L1_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21003..21908)
                     /gene="yijE"
                     /locus_tag="ACH3L1_000476"
     CDS             complement(21003..21908)
                     /gene="yijE"
                     /locus_tag="ACH3L1_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFT4075368.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22002..24182)
                     /gene="katG"
                     /locus_tag="ACH3L1_000477"
     CDS             complement(22002..24182)
                     /gene="katG"
                     /locus_tag="ACH3L1_000477"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFT4075369.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24511..25401)
                     /gene="metF"
                     /locus_tag="ACH3L1_000478"
     CDS             complement(24511..25401)
                     /gene="metF"
                     /locus_tag="ACH3L1_000478"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFT4075370.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25477..25611
                     /locus_tag="ACH3L1_000479"
     CDS             25477..25611
                     /locus_tag="ACH3L1_000479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075371.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25750..28182)
                     /gene="metL"
                     /locus_tag="ACH3L1_000480"
     CDS             complement(25750..28182)
                     /gene="metL"
                     /locus_tag="ACH3L1_000480"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFT4075372.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28185..29345)
                     /gene="metB"
                     /locus_tag="ACH3L1_000481"
     CDS             complement(28185..29345)
                     /gene="metB"
                     /locus_tag="ACH3L1_000481"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFT4075373.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29622..29939
                     /gene="metJ"
                     /locus_tag="ACH3L1_000482"
     CDS             29622..29939
                     /gene="metJ"
                     /locus_tag="ACH3L1_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFT4075374.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30123..30731
                     /locus_tag="ACH3L1_000483"
     CDS             30123..30731
                     /locus_tag="ACH3L1_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFT4075375.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30792..31004)
                     /gene="rpmE"
                     /locus_tag="ACH3L1_000484"
     CDS             complement(30792..31004)
                     /gene="rpmE"
                     /locus_tag="ACH3L1_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFT4075376.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31207..33405
                     /gene="priA"
                     /locus_tag="ACH3L1_000485"
     CDS             31207..33405
                     /gene="priA"
                     /locus_tag="ACH3L1_000485"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFT4075377.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33561..34586
                     /gene="cytR"
                     /locus_tag="ACH3L1_000486"
     CDS             33561..34586
                     /gene="cytR"
                     /locus_tag="ACH3L1_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFT4075378.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34678..35637
                     /gene="ftsN"
                     /locus_tag="ACH3L1_000487"
     CDS             34678..35637
                     /gene="ftsN"
                     /locus_tag="ACH3L1_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFT4075379.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35730..36260
                     /gene="hslV"
                     /locus_tag="ACH3L1_000488"
     CDS             35730..36260
                     /gene="hslV"
                     /locus_tag="ACH3L1_000488"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFT4075380.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36270..37601
                     /gene="hslU"
                     /locus_tag="ACH3L1_000489"
     CDS             36270..37601
                     /gene="hslU"
                     /locus_tag="ACH3L1_000489"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFT4075381.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37668..38594
                     /gene="menA"
                     /locus_tag="ACH3L1_000490"
     CDS             37668..38594
                     /gene="menA"
                     /locus_tag="ACH3L1_000490"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFT4075382.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38687..39172
                     /gene="rraA"
                     /locus_tag="ACH3L1_000491"
     CDS             38687..39172
                     /gene="rraA"
                     /locus_tag="ACH3L1_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFT4075383.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39257..39502)
                     /gene="zapB"
                     /locus_tag="ACH3L1_000492"
     CDS             complement(39257..39502)
                     /gene="zapB"
                     /locus_tag="ACH3L1_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFT4075384.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39928..40773
                     /gene="glpF"
                     /locus_tag="ACH3L1_000493"
                     /pseudo
     CDS             39928..40773
                     /gene="glpF"
                     /locus_tag="ACH3L1_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40813..42303
                     /gene="glpK"
                     /locus_tag="ACH3L1_000494"
     CDS             40813..42303
                     /gene="glpK"
                     /locus_tag="ACH3L1_000494"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFT4075385.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42533..43543
                     /gene="glpX"
                     /locus_tag="ACH3L1_000495"
     CDS             42533..43543
                     /gene="glpX"
                     /locus_tag="ACH3L1_000495"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFT4075386.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43640..44386
                     /gene="fpr"
                     /locus_tag="ACH3L1_000496"
     CDS             43640..44386
                     /gene="fpr"
                     /locus_tag="ACH3L1_000496"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFT4075387.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44391..44819)
                     /gene="uspD"
                     /locus_tag="ACH3L1_000497"
     CDS             complement(44391..44819)
                     /gene="uspD"
                     /locus_tag="ACH3L1_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFT4075388.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44846..45145)
                     /locus_tag="ACH3L1_000498"
     CDS             complement(44846..45145)
                     /locus_tag="ACH3L1_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFT4075389.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45357..45797)
                     /locus_tag="ACH3L1_000499"
                     /pseudo
     CDS             complement(45357..45797)
                     /locus_tag="ACH3L1_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45898..46497
                     /locus_tag="ACH3L1_000500"
     CDS             45898..46497
                     /locus_tag="ACH3L1_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFT4075390.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46605..47372
                     /gene="tpiA"
                     /locus_tag="ACH3L1_000501"
     CDS             46605..47372
                     /gene="tpiA"
                     /locus_tag="ACH3L1_000501"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFT4075391.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47427..48182)
                     /gene="cdh"
                     /locus_tag="ACH3L1_000502"
     CDS             complement(47427..48182)
                     /gene="cdh"
                     /locus_tag="ACH3L1_000502"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFT4075392.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48289..49278)
                     /gene="sbp"
                     /locus_tag="ACH3L1_000503"
     CDS             complement(48289..49278)
                     /gene="sbp"
                     /locus_tag="ACH3L1_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFT4075393.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49598..50560)
                     /gene="pfkA"
                     /locus_tag="ACH3L1_000504"
     CDS             complement(49598..50560)
                     /gene="pfkA"
                     /locus_tag="ACH3L1_000504"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFT4075394.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50741..51643)
                     /gene="fieF"
                     /locus_tag="ACH3L1_000505"
     CDS             complement(50741..51643)
                     /gene="fieF"
                     /locus_tag="ACH3L1_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFT4075395.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51791..52291)
                     /gene="cpxP"
                     /locus_tag="ACH3L1_000506"
     CDS             complement(51791..52291)
                     /gene="cpxP"
                     /locus_tag="ACH3L1_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFT4075396.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52441..53139
                     /gene="cpxR"
                     /locus_tag="ACH3L1_000507"
     CDS             52441..53139
                     /gene="cpxR"
                     /locus_tag="ACH3L1_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFT4075397.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53136..54509
                     /gene="cpxA"
                     /locus_tag="ACH3L1_000508"
     CDS             53136..54509
                     /gene="cpxA"
                     /locus_tag="ACH3L1_000508"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFT4075398.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54615..55289)
                     /gene="yiiM"
                     /locus_tag="ACH3L1_000509"
     CDS             complement(54615..55289)
                     /gene="yiiM"
                     /locus_tag="ACH3L1_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFT4075399.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13493 a        15019 c        14164 g        12745 t
ORIGIN      
        1 ccagaatgct gacaaatttc tgcctgatgc ggcgtaaacg ccttatccgc ctacgaagtg
       61 tcgcactttg taggcgtggt aaactcccca ttttcagggg agcaatataa acaaattact
      121 ctaaccgagc ctgcgcaaaa gcaatcgcct gcgccacctg ctgcggtgag acgccgcctt
      181 ttgccgcacg cttttcgagg cacgattgta gcgacagaat cggatagaca tcttcgccaa
      241 tcacctgact gaatttctgc aactcactga gtggcagatc ttccagcggt ttgccctgac
      301 gaatggcttc caccaccgct tcacccacaa tatggtgcgc ctcgcggaaa ggtacgcctt
      361 tcgccaccag gtaatccgcc agttcagtgg cgttcgcata cccctgctgc gccgcttcct
      421 gacaacgcgg acgtttcacc tgaatgccgt ccagtaccag cgccgccata tgcaggcagt
      481 ccagccaggt atcgagcgcg tcgaacagac cttctttgtc ttcctgcata tctttgttgt
      541 aagccagcgg caaacctttc agcgtcatca tcatgccggt taacgcaccc tgcacccggc
      601 cgcatttacc gcgaatcagc tccagcgcat ccgggttttt cttctgcggc attaatgatg
      661 aaccggaagt cacgcggtcc gaaagctcca caaaccccgc ttcgccggtg ttaaagaaaa
      721 tcagatcttc agcaaaacgc gacagatgca ccatgccgat agcggcagca gaaagcagtt
      781 ccaacacatg gtcacggtca gaaacgctgt ccagactgtt acgggtcgcc gaagcaaagc
      841 ccagccagcc tgctaactgt tcacggtcga tttcataggc cgttcccgcc agcgcgccac
      901 agcctaatgg gctgacatcc agacgcttaa gcgcatcctg caaacggctt tcatcacgcg
      961 ccagcatctc aacataggcc aggcaccagt gcgcgaacgt caccggctgg gcgcgttgca
     1021 ggtgagtgta acctggcatt accgcgtcct gattgttttg tgcggtttcc accagcgccg
     1081 attgcagctg ccggttagcc gtcagtaact cgctaacggt atctttgcac cacagtttca
     1141 ggtcagtcgc tacctgatca ttacggctac gcccggtatg cagctttttg cctaactggc
     1201 ccactttgtc gatcagtttg ccttccaccc agctatggat atcttcggcg tcgctttcaa
     1261 ggatttgttg tggcctggcg cgaacatctt ccagcaacac gttcagcgcc tcttccagtt
     1321 gcgcctgctc ttctgcggtt aacacgccga ccgttaccaa ggctttggac caggccacag
     1381 agccaacaat atcctgctcc gccagacggt aatcaaagcg cagtgagtcg ttgaattgtt
     1441 tgaaccgttg atctgctgcc tgggtaaaac gcccgcccca aagtgccata actctgtttc
     1501 cttattttga aattcaatgc cggaaagctg cgcctccggc taacaaaact taagctaaaa
     1561 tccgcgtacc catcggcata ccgttaaaca gtgccggaag ctgctccgca taacgccaag
     1621 aagcgatatc taccggacgg cccagcgtgc gggccgcatc cagcgccgcg ttcactttca
     1681 ctatcatgcc gtcagtaata atgccctgct caatcagttg ttctgctttc gcggcggtca
     1741 tttcggcaat gcgttgccct ttgccgtcga gaatgccgct gacgtcggag agcaaaatca
     1801 gatccgcgcc cagcgttgcc gccagcgccg ttgccgcctg gtcggcattg acgttcatca
     1861 gttgcccttc gtctgttacg ccaatggagc tgaccaccgg cagataaccg ttctccagca
     1921 aggagttgat aagcttaggc gaacctggct gcgccagtcc aacatgacct aactcttcat
     1981 caagctgggt cactttgacg ctgtcgccgt caccgagaaa caaacctacg gccgcaatct
     2041 gatgtttctt cgcccatgcc aacagggttt tatttgccgt tcccgccagt gctccggtga
     2101 taatgtctat ctgatcagca ggcgtcaccc gcaggccgtt tttctttttc accggcagat
     2161 tcagcccttt catcagctca tccaccacgc aaccgccgcc gtgcacaatc accagcggac
     2221 gctgatgtga ctcacgataa ttcaccaatg cgctaaacag acgttccagc gcctcttcac
     2281 tatccagcag tacgccgccc agtttgataa ttaatggatt catcattgca cccttaaata
     2341 agagactgcg tttccgcata gccgaaacga atattggcgc actgtaccgc ttgtgccgcc
     2401 gcgcctttca gtaagttgtc ttcggtcgcc acaataatca gatgctcgcc ctgaacggca
     2461 aacccgatat cgcaaaatgg cagcccaacg acatttttca gcgccggaac gcctttgtca
     2521 tacagccgca ccagcggttt atgggcatac gcctgctgta acgcttgcgc gacttgcgcc
     2581 tgggtcacac ccgatttcag gcggcaggta atggtttcga gaatgccgcg cgggaaattg
     2641 cccagatgtg gggtgaagat aacgtcagca ccgaggtgtg tggcgatctc tggttgatgg
     2701 cgatgagtaa agacgccata cggttgcagg ctaacttcac aaaaactatt tgaaatcgct
     2761 gccttacgtc ctgcaccgct cacgccgctg gtggcgttga tcaccggcca ctgattgagg
     2821 tcaagaagat cggcatcaat caacggtttc agcgccagct gtgccgccgt cggataacag
     2881 cctggcaccg caatcaaatt cgcttctttt aatttattac cgcaccactc cgccagaccg
     2941 taggctgcct gttccagcag ttccgggtat tgatgggtaa agccgtaata tttttcatag
     3001 aaggtggcgt cgttaacacg aaacgcgccg gaaaggtcga acaccacgca gcccgcttca
     3061 agaaactgcg gcgctaaatc gtggctaact tcatgggcgg tggcgagaaa cactacgtcc
     3121 acccctgggc taaactcgct gatatccgac atcggctgca acggcagctc aacgatgcct
     3181 tttagctgcg gatgcaaatc ggagattaac tttcccgcat cattgctttg cgctgaaaca
     3241 gtcaaagcgg ttatgttcat atgcggatgg cgatttacat aggtcactag ctctgcgcca
     3301 gcgtagccgc tggcacccac aatcagcgta ttcaacatcg gggctattca ccttcttatg
     3361 tctggttgcc aggttaaacg taaaacattc accttacggc tggtgggttt tattacgctc
     3421 aacgttagtg tatttttatt cataaatact gcatgaatat tgatactatc atgaccagag
     3481 gtgtgtcaac aatgaaaaac aaattaccgc catttatcga gatttaccgc gctctgattg
     3541 ccacaccttc aataagcgct acggaagagg cactcgatca aagcaatgca gatttaatca
     3601 ctctgctggc ggactggttt aaagatttgg gcttcaatgt ggaagtgcag cctgttccag
     3661 gaactcgcaa caaatttaat atgctggcca gttacggaca gggggctggc ggcttgttgc
     3721 tggcggggca taccgatacg gtgccatttg atgacggtcg ctggacgcgc gatccgttta
     3781 cactgacgga gcatgacggc aagctttacg gcttaggcac cgccgacatg aaaggctttt
     3841 ttgcgtttat ccttgatgcg ctacgcgatg tcgacgtcac gaaactgaaa aaaccgctct
     3901 acattctggc gactgctgat gaagaaacca gtatggccgg agcgcgttat tttgccgaaa
     3961 ctaccgccct gcgcccggat tgcgccatca ttggcgaacc gacgtcacta caaccggtac
     4021 gcgcacataa aggtcatatc tctaacgcca tccgtattca gggccagtcg gggcactcct
     4081 gcgatccagc acgcggaatt aacgctatcg aactaatgca cgacgccatc gggcatattt
     4141 tgcaattgcg cgataacctg aaagaacgtt atcactacga agcatttacc gtgccatacc
     4201 ctacgctcaa cctcgggcat attcacggtg gcgacgcttc taaccgtatt tgcgcttgct
     4261 gtgagttgca tatggatatt cgtccgcagc ctggcatgac actcaatgaa cttaatggtt
     4321 tgctcaacga tgcattggct ccggtgagcg aacgctggcc gggtcgtctg acggtcgacg
     4381 agctgcatcc gccgatccct ggctatgaat gcccaccgaa tcatcaactg gttgaagtgg
     4441 ttgagaaatt gctcggagca aaaaccgaag tggtgaatta ctgtaccgaa gcgccgttta
     4501 ttcaaacgtt atgcccgacg ctggtgctcg gtccgggttc catcaatcaa gctcatcagc
     4561 ctgatgaata tctggagacg cggtttatta aacccacccg tgagttgatt atccaggtaa
     4621 ttcaccattt ttgctggcat taaaacgtag gccggataag gcgctcgcgc cgcatccggc
     4681 accgttgcca aactccggtg ccgcaataat gtcggatgcg atgcttgcgc atcttatccg
     4741 acctacacct ttggtgttat gtatggcgat tttttaacat ttccataagt tacgcttatt
     4801 taaagcgtcg tgaatttaat ggcgtaaatt cctgctattt attcgtttgc tgaagcgatt
     4861 tcgcagcatt tgacgtcacc gcttttacgt ggctttataa aagacgacga aaagcaaagc
     4921 ccgagcatat tcgcgccaat gcgacgtgaa ggatacaggg ctatcaaacg ataagatggg
     4981 gtgtctgggg taatatgaac gaacaatatt ccgcattgcg tagtaatgtc agtatgctcg
     5041 gcaaagtgct gggagaaacc atcaaggatg cgttgggaga acacattctt gaacgcgtag
     5101 aaactatccg taagttgtcg aaatcttcac gcgctggcaa tgatgctaac cgccaggagt
     5161 tgctcaccac cttacaaaat ttgtcgaacg acgagctgct gcccgttgcg cgtgcgttta
     5221 gtcagttcct gaacctggcc aacaccgccg agcaatacca cagcatttcg ccgaaaggcg
     5281 aagctgccag caacccggaa gtgatcgccc gcaccctgcg taaactgaaa aaccagccgg
     5341 aactgagcga agacaccatc aaaaaagcag tggaatcgct gtcgctggaa ctggtcctca
     5401 cggctcaccc aaccgaaatt acccgtcgta cactgatcca caaaatggtg gaagtgaacg
     5461 cctgtttaaa acagctcgat aacaaagata tcgctgacta cgaacgcaac cagctgatgc
     5521 gtcgcctgcg ccagttgatc gcccagtcat ggcataccga tgaaatccgt aagctgcgtc
     5581 caagcccggt agatgaagcc aaatggggct ttgccgtagt agaaaacagc ctgtggcaag
     5641 gcgtaccgaa ttacctgcgc gaactgaacg aacaactgga agagaatctc ggctacaaac
     5701 tgcccgtcga atttgttccg gtacgtttta cctcgtggat gggcggtgac cgcgacggca
     5761 acccgaacgt cactgccgat atcacccgcc acgtcctgct gctcagccgc tggaaagcca
     5821 ccgatctgtt cctgaaagat attcaggttc tggtttctga actgtcgatg gttgaagcga
     5881 cccctgaact gctggcgctg gttggcgaag aaggtgccgc agaaccgtat cgctatctga
     5941 tgaaaaacct gcgttctcgc ctgatggcga cacaggcatg gctggaagcg cgcctgaaag
     6001 gcgaagaact gccaaaacca gaagggctgc tgacacaaaa cgaagaactg tgggaaccgc
     6061 tctacgcttg ctaccagtca cttcaggcgt gtggcatggg tattatcgcc aacggcgatc
     6121 tgctcgacac cctgcgccgc gtgaaatgtt tcggcgtacc gctggtccgt attgatatcc
     6181 gtcaggagag cacccgccat accgaagcgc tgggcgagct gacccgctac ctcggtatcg
     6241 gcgactacga aagctggtca gaggccgaca aacaggcgtt cctgatccgc gaactgaact
     6301 ccaaacgtcc gcttctgccg cgcaactggc aaccaagcgc cgaaacgcgc gaagtgctcg
     6361 atacctgcca ggtgattgcc gaagcgccgc aaggctccat tgccgcctac gtgatctcga
     6421 tggcgaaaac gccgtccgac gtactggctg tccacctgct gctgaaagaa gcgggtatcg
     6481 ggtttgcgat gccggttgct ccgctgtttg aaaccctcga tgacctgaac aacgccaacg
     6541 atgtcatgac ccagctgctc aatatcgact ggtatcgcgg cctgattcag ggcaaacaga
     6601 tggtgatgat tggctattcc gactcagcaa aagatgcggg cgtgatggca gcttcctggg
     6661 cgcaatatca ggcacaggat gcattaatca aaacctgcga aaaagcgggt attgagctga
     6721 cgttgttcca cggtcgcggc ggttccattg gtcgcggcgg cgcacctgcc catgcggcgc
     6781 tgctgtcaca accgccagga agcctgaaag gtggcctgcg cgtgactgaa cagggcgaga
     6841 tgatccgctt taaatatggt ctgccagaaa tcaccgtcag cagcctgtcg ctttacaccg
     6901 gggcgattct ggaagccaac ctgctgccac cacctgagcc gaaagagagc tggcgtcgca
     6961 ttatggatga actgtcagtc atctcctgcg atctctaccg tggctacgta cgtgaaaaca
     7021 aagattttgt gccttacttc cgctccgcta cgccggaaca agaattgggc aaactgccgt
     7081 tgggttcacg tccggcgaaa cgtcgcccaa ccggcggcgt cgagtcgctg cgcgccatac
     7141 cgtggatttt tgcctggacg caaaaccgcc tgatgctccc cgcctggctg ggtgcaggta
     7201 cggcgctgca aaaagtggtc gaagatggca aacagagcga gctggaagcc atgtgccgcg
     7261 attggccatt cttctcgacg cgtctcggca tgctggagat ggtcttcgcc aaagcagacc
     7321 tgtggttggc ggaatactat gatcaacgtc tggtagacaa agcactgtgg ccgttaggta
     7381 aagagttacg caacctgcaa gaagaagaca tcaaagtggt gctggcgatt gccaacgatt
     7441 cccatctgat ggccgatctg ccgtggattg cagagtctat tcagctacgg aatatttaca
     7501 ccgacccgct gaacgtattg caggccgagt tgctgcaccg ctcccgccag gcagaaaaag
     7561 aaggccagga accggatcct cgcgtcgaac aagcgttaat ggtcactatt gccgggattg
     7621 cggcaggtat gcgtaatacc ggctaatctt cctcttctgc aaaccctcgt gcttttgcgc
     7681 gagggttttc tgaaatactt ctgttctaac accctcgttt tcaatatatt tctgtctgca
     7741 ttttattcaa attctgaata taccttcaga tatccttaag gaattgtcgt tatattcggc
     7801 gatatttttt tcaagacagg ttcttactat gcattccaca gaagtccagg ctaaacccct
     7861 ttctagctgg aaagccctgg gttgggcact gctctacttt tggtttttct ctactctgct
     7921 acaggccatt atttacatca gtggttatag tggcaccaac ggcattcgcg actcgctgtt
     7981 attcagctcg ctgtggttga tcccggtatt cctctttccg aagcggatta aaattattgc
     8041 cgcagtgatc ggcgtggtgc tatggacggc ctctctggcg gcgctgtgct actacgtcat
     8101 ctacgggcag gagttctcgc agagcgttct gtttgtgatg ttcgaaacca acaccaacga
     8161 agccagcgag tatttaagcc agtatttcag cctgaaaatt gtgcttatcg cgctggccta
     8221 tacggcggtg gcagttctgc tgtggacacg cctgcgcccg gtctatattc caaagccgtg
     8281 gcgttatgtt gtctcttttg ccctgcttta tggcttgatt ctgcatccga tcgccatgaa
     8341 tacgtttatc aaaaacaagc cgtttgagaa aacgttggat aacctagcct cgcgtatgga
     8401 gcctgccgca ccgtggcaat tcctgaccgg ctattatcag tatcgtcagc aactaaactc
     8461 gctaacaaag ttactgaatg aaaataatgc cttgccgcca ctggctaatt tcaaagatga
     8521 atcgggtaac gaaccgcgca ctttagtgct ggtgattggc gagtcgaccc agcgcggacg
     8581 catgagtctg tacggttatc cgcgtgaaac cacgccggag ctggatgcgc tgcataaaac
     8641 cgatccgaat ctgaccgtgt ttaataacgt agttacgtct cgtccgtaca ccattgaaat
     8701 cctgcaacag gcgctgacct ttgccaatga aaagaacccg gatctgtatc tgacgcagcc
     8761 gtcgctgatg aacatgatga aacaggcggg ttataaaacc ttctggatca ccaaccagca
     8821 gacgatgacc gcccgcaata ccatgctgac ggtattttcg cgccagaccg acaagcagta
     8881 ctacatgaac cagcaacgta cgcagagtgc gcgtgaatac gacaccaacg tgctgaagcc
     8941 gttccaggat gtgctgaatg accctgcgcc gaagaaactg atcatcgttc atctgctggg
     9001 tacgcatatc aaatacaaat accgctaccc ggaaaatcag ggcaagtttg atggcaatac
     9061 cgatcatgtt ccgccaggat taagcgcaga agagctggaa tcatataacg attatgacaa
     9121 cgctaacctg tataacgatc atgtggttgc cagcctgatt aaagacttta aagcggcaga
     9181 cccgaacgga ttcctggttt acttctctga ccacggtgaa gaggtttacg acacgccgcc
     9241 gcacaaaact caggggcgta acgaagacaa cccgacgcgc cacatgtaca ccattccgtt
     9301 cctgctgtgg acgtcggaaa aatggcaagc gactcatccc cgtgatttct cacaggatgt
     9361 cgatcgtaaa tacagcctgg cggaactgat ccacacctgg tcagatttgg cgggcttatc
     9421 ttacgacggt tacgatccaa cccgttcagt ggtgaatccg cagttcaaag aaactacccg
     9481 ctggattggt aacccgtaca agaaaaacgc gctgatcgat tacgacactc tgccgtatgg
     9541 cgatcaggtg ggtaatcagt agatgacctt tgtatcgcgt ctggcgctat ttgccggatg
     9601 cgatactgcc gcaatgttta catcgaactc ccctcaggcc acacttttca ggtggccttt
     9661 tttaataatg ccagttactg ttttcccgtc tacataccta agggaataac atgtatcacg
     9721 acgtcagcta tctgctctcc cgcctgatca acggcccgct gtccctgcgc cagatttact
     9781 ttgccagcag taacggcccg gtgcccgatc ttgcgtatca ggtcgatttt ccacggctgg
     9841 aaattgtgct ggaaggtgag tttgttgata ccggcgctgg agcaacgtta gttcccggcg
     9901 atgtgctgta cgttgccgct ggtggctgga atttcccaca atggaaaaca cccgctacca
     9961 cctttagcgt gctgtttggc aaacagcaac tcggcttcag cgtcgtgcaa tgggatggca
    10021 aacaatatca aaatctggcg aagcaacacg tcgcccgacg cggcccacgc ataggttctt
    10081 ttctgctaca aacgctcaat gaaatgcaaa tgcagccgca ggagcagcaa acggcaaggc
    10141 ttatcgtagc cagcctgctt agccactgcc gcgatttgct cggcagccaa atccagaccg
    10201 cctcacgcag ccaggcacta tttgaagcta ttcgcgatta tatcgacgaa cgctatgcct
    10261 ccgcgcttac ccgcgaatct gtcgcacagg cattctatat ttcaccaaac tatctctcgc
    10321 acctgtttca aaaaacgggg gccattggtt ttaacgaata cctgaatcac acgcgactgg
    10381 agcacgctaa gacgttattg aaaggctatg acctgaaggt aaaagaagtg gcgcacgcct
    10441 gcggctttgt cgacagcaac tacttctgcc gtctgtttcg taaaaacacc gaacactcgc
    10501 cgtcggaata tcgccggcag taccacagcc aactgacaga aaaaccgact actccagaat
    10561 aagatgggtt tgctgcggcg cagaaagcac tttgcgcacc gcgctcatta cccgctgcgg
    10621 ctcacgcagg aatgcgtaga tgctgcattg cacatagcgg caatgttcaa atcgctcggc
    10681 accggcaagc tcgatatccg taatcaacag agcaacatcc gcccgacgga tatcctcgtc
    10741 cgctaaacga ttctccgttc ccagcgcgcc ctgagtttca atgctgacgc cccacttctc
    10801 taactggcac aacttttcca gccgttccgc cgccatataa gtatgcgcca cgccgcttac
    10861 gcaggcggtt actgccacca ggtatgccat tttaacctcc cacggtaacc tgaaatccgg
    10921 cccgttctgc catttcgcgc atcgttgtca catcggctga cgacggcgca ggcacctctt
    10981 tcatcgacca tgttttcccc agcaggcggt atttcggttc gccgtactga tgaaacggta
    11041 acagatggat ctgcctgatt ttcagcggga tcagtacatc cagcgcctgc tgcatattct
    11101 cccggctgag cgtgaaacca gggatcaacg gtaaacgcgg gatcacgttg acaccctcac
    11161 tcaccagcaa acgcagattc tccagcacgc gtggcaggtt catcttcacc acatcccgcg
    11221 cctgagtcgc gtccataatt tttaaatcga acagcacttc atcgcacaat ttcgccagcg
    11281 gtaacagctt gcttgctggc gcgtctccgg cagtttcaat ggcgcatgag acaccccaca
    11341 gccgcagtcg ctgtaaaaaa cgggtagcaa actccgcctg cattaacact tcgccgccag
    11401 aaagcgtgac gccgccgccg gacgtgcgaa agaaaatgtc atctttcatc acttcccgtt
    11461 ccagagcgtc aaggctgata tcgcgaccaa tccgttcaaa cgccccggag gggcattcat
    11521 ccgcatcacg caaacatttc gcacagtgca gacatttcgc ctctctgcgt accgtctgga
    11581 ttttgccgga gatcgactcc ggattagcac accacgggca aagatgcgga cagcctttaa
    11641 aaaagaccac cgtacgaatg ccctcgccgt cattcagtga ataacgctga atgttgaaaa
    11701 tgcgcgccac atcatcgcgg cgcgtttcca caacgttaca gctgatgcgc tgtccggcgg
    11761 atgatgtcat cctggatctc cttcgacagt tcgacaaaga aggcgctgta tccggcaacg
    11821 cgcaccacca gcccggcata atcttgtggg cgctgttgcg cttcccgcaa cgtgtcggcg
    11881 ttcaccacgt taaactgaat atgctgcaac ttaagctggg taaacacccg taagaagtcg
    11941 gccagtttgc gtaatcccgc ttcaccttcc agggtcgccg gagtgaattt cacgttcagc
    12001 aacgtaccgt tagacagcag cgtattatcg agcttactga ctgacttcag taccgccgtt
    12061 ggcccttgtg cgtcctgacc cagcataggt gacaagccgc cgtctgccag ttgttccccg
    12121 gcaaaacgac cgtctggcgt cgcgccaacc accgatccca acggaacgtg agcagaaacg
    12181 gtatacgatc ccggcgtgaa gtaaccgccg cgcgggttct ggtatttttc cacttctttg
    12241 cagtagtggc gcaacagttc ggcgctaatg ttatccacct cgtcgatatc gttaccgtat
    12301 ttctcaaagc ggttaattaa gcgagcgcgg actttttcgc cttctggcgt tgcaaagttg
    12361 gcttttaata ccgacagcaa ttcgtcaaaa cttaaacgct gttgctcaaa aaccatccct
    12421 ttcaacgcat ggagagaatc gctcaggttg gcgataccaa ttccctgtac gccggagaag
    12481 ttataacgcg cgccgccatc ggtaatatcg cgaccttttt ccagacaatc gctgataaaa
    12541 gacgagagca gcggtacagg tgcccagtcg cgatggccga tatcacaaat attgctgccc
    12601 tcaaccatca gggtgatgta gtggctgatc ttggcgcgga tctgctccag taaaccttca
    12661 taagtcagcg cagcattgcc ttcattttca tgcaggcaga tttccatcac tttcagcaga
    12721 ttaaacatcg cgatgtcatg caagccgtag gttctgccgg gaatagataa ttccacacag
    12781 cccactacgg aatagtcgcg cgcatcttcc agcgaaacgc cacggttgag gaacgctggc
    12841 accaccactt catcgttaaa gatttgcgga ataccggtgc cgaggcgaat ggtttcggcg
    12901 gttttcatca ggaacggcgt gtcgatcaag gcgttagtgc gcacgccgag gttcggttgc
    12961 ggtaattgca cgctttgata ggcgtcaagg caaaggaacg aaagcacgtt caccgcgcta
    13021 cgtccgttct cggttaaccc gccgagcagt gcggtatagc cggtcgggaa accggcgaaa
    13081 taacgcgcgc tactggtgga gcgcaacagc acgatgtcgt tgcatttcac ccataaagat
    13141 tcgagcagtt ctttcaggaa cgccggatct tcgccctggg ttaaagatgc ctgatagaac
    13201 ggcaacatat actggtcgaa gcgccctaaa gacagcgaac tggcgttgga ttcgtattgc
    13261 agaatgatgt tcatgtacca gaataactgg cacgcctgcc agaacgtctg cggcttatgt
    13321 tgcgcgttat ggcgggagat ctccgcaata gtcagcagct cttcgcgacg ctgggcatct
    13381 gtgcagtttg ccgccatcgt ttccgccagt tcggcgtaac gcaaaatgtg tttctgcgag
    13441 gcttccagca gtaacaacgc ggcctgataa aagtgattct ccggctgttg ctgacaatgt
    13501 tgctgcatct gtgccaccag ctcacccaga ccgtgattca acaggcgtgg gtaatcaata
    13561 ataatgtgcc cctggccttt atccgtctgg ttgatgctga aaatctgcgt gttggtcgcg
    13621 gcttttactt catctgtcat ctgcccgttg atgaaatctt tcatcgaacg tttttcccag
    13681 tacgggaaca actcttcacg gtagatacgt ttatcttctt cgctgatagc aaagcggtcc
    13741 tgcggacgcg tcgggaattg atccagctct ttcagcagcc agtaagggtc catttccggc
    13801 gacataatcc cggcgcgcgg ttttacggtg cggttaccgg caatcagttc ttcatcacga
    13861 atcgaaattt caacatgttc aaggatatac gctgttgctt tcgcccggcg caatatcacc
    13921 ggttcgcctt cggtttgccg atggctggcg gtataaagca gcgcccgctc cagcgagatt
    13981 tcacgggtat tggcaaacag tgcagttttg aggcgagaga tacgattcgt catgaggaac
    14041 tcctgtggga aagcgaaccc gcccttgcga gcgggagaag aacttaagcg gtttgcgcca
    14101 ggtgagcttc aattttgctc ataatggcgt cagcacgttt tactgcatcg ctgatgttga
    14161 cgcgcacgat ggtcttgcca gcaaaacgct cttcaaactt gatgccgata tctttggtca
    14221 gaatcaccat atcagcgctc gccacgtctt ccgcagtcag ttcattttcc agaccaattg
    14281 acccctgggt ttctactttc acttcccagc ctttcgcttt cgcggcactt tccagcgcct
    14341 ctgctgccat ataggtatgg gcaacacctg aaggacatgc ggttactgca ataattttcg
    14401 tcatggctcg ttcctcaatt aattaatttc aaaatccaaa tccaggtcgt cttctttttc
    14461 atcagtcgaa gacccatttt tacgcgccag acttttcagc acgttcacac aaacagccgt
    14521 caccactgct ccgacagcca ctgctgcgat ataacccaac ttaccttcaa ccaccggcag
    14581 cacaatcaga ccaccccagc ctgcatagca ctgcgcaccg accagcgccg cagttactgc
    14641 accacaaact gaaccgacca taatggaagg aatgacacgc agcggatcgg cagcggcgaa
    14701 aggaatcgcc ccttcagtaa cgccaacaca gcccatcacc agtgccgctt tgccggtttc
    14761 gcgctcttct gcggagaaat ttttacgacc aatcagcgtc gccagcccca tccccagcgg
    14821 cgggatacaa atacccacgg cagcaatggc caccacggta taaacaccct gagcaacgca
    14881 aatcagcatg aaggcatagg ccactttgtt aaccggaccg cccatatcga acgccagcat
    14941 cagccccatg atcaccgcca gcataacaat gctgccctgc tgcatcccct gaagccactg
    15001 agtcaggctg ttggtcaacg cccctacagg ctcgcccaag ccccacatca tgatgcctgc
    15061 ggtaatcagt gtgccgatga taggaataat gaagatgggc atcaccgagc gcagaacttt
    15121 atgcaccgga attttcttca ggtaatgcac cacgatgccg ccgataatcc cggcgatcag
    15181 cgcaccaaag aagcccgcac caaagctgtt accaacccag gcaccgatag cgcacggagc
    15241 cagcgcagaa cgctctgcaa tggagtaacc aatgtaagcg gcgaggaaag gcaccatcag
    15301 cgtcaaacct gcaacgccga tatcaaacag ttttttcaaa ttcggatcgg ctacggcatc
    15361 cggcactgcg cctttgccat acaacatgac ggaaaccgcc agcaaaatac cgcccgatac
    15421 cacgaaggga atcatgtgtg aaacgcccgt cattaaatgc tgacgggtat ttttcaggat
    15481 ctgcaccaac tcattcataa gctgctcccg ggcttgcaaa agcccatgtc catatttttg
    15541 atggaggaaa caataggcaa tcgcaggcgg agcaaacagt ggataaaaag gccatttact
    15601 ggacttttgt aatatcagta caaaaatgcg atccgcctca taacttgcga taatccggaa
    15661 gcatgccgca aaacggcgtc ttgtgagacg cgtcgcaaat tgacggttac attacatttg
    15721 tccagctttt ggcagatttg tcacatggca ggccgttacc gtgcgctctt atcctgtaaa
    15781 cagaaaaccg taccgggaga aaggcaatgg ccctgattgt ggaatttatt tgtgagctac
    15841 ctaacggcgt acatgcgcgt ccggcaagcc acgttgaaac gctgtgtaat actttttcat
    15901 ctcaaattga gtggcataac ctgcgtactg accgtaaagg caacgccaaa agcgctctgg
    15961 cgctgattgg caccgatacg ctggtgggtg ataactgcca gctactgatt tccggggccg
    16021 acgaacagga agggcaccag cgtttaagcc aatggctgcg cgatgaattc ccccactgcg
    16081 atgcgccgct ggcggaagtt aaatctgacg aactggaacc actgccggtt tcactgacca
    16141 atctgaatcc gcaaattatc cgcgcccgca ccgtgtgcag cggtagtgca ggcggcattc
    16201 tgacgccgat ctcttcttta gatctcaatg cgctgagtaa tcttcccgca gccaaagacg
    16261 ttgacgccga gcaatccgca ctggaaaacg gcctgacgct ggtactgaaa aacattgagt
    16321 ttcgtctgct ggatagcgac ggtgctacca gcgcgattct ggaagcgcac cgatccctgg
    16381 ctggcgatac ttccctgcac gaacatttac tggcaggcgt cagcgccgga ttaagctgcg
    16441 ccgaagcgat tgtcgccagt gcgaatcact tttgcgaaga gtttgcccgt tccagcagca
    16501 gatacctgca agaacgtgcc ctggacgtac gcgacgtctg cttccagtta ctccagcaaa
    16561 tctacggtga gcaacgcttc ccggcaccgg gcaaactgac gcagtccgcc atttgtatgg
    16621 ctgatgaact gacccccagc cagttcctcg aactggataa aaatcacctc aaaggattgt
    16681 tactgaaaag cggcggcacc acctcacata cggtgatcct tgcccgttcg ttcaacattc
    16741 cgacgctggt tggtgtggat attgatgccc tcactccgtg gcagcatcaa acgatttata
    16801 tcgacggcaa cgccggggcg attgtggttg agccaggaga agccgtagct cgttattatc
    16861 agcaagaagc ccgcgtacag gacgccctgc gtgagcaaca gcgtgtctag ctgacccaac
    16921 aagcccgtac cgctgacggt atccgcattg aaattgccgc taacatcgct cactccgtgg
    16981 aagcgcaggc cgcattcggc aatggtgcgg aaggcgttgg tttgttccgc actgaaatgc
    17041 tctatatgga tcgcaccagt gcaccgggcg aaagcgagct gtacaacatt ttttgtcagg
    17101 cgctggagtc tgccaacgga cgcagcatta ttgtgcgcac tatggacatt ggcggcgaca
    17161 aacccgttga ttatctaaac attcccgcag aggcaaaccc gttcctcggt tatcgcgccg
    17221 tgcgtattta tgaagaatac gcgtcgttat ttaccacaca actacgctcg atcctgcgcg
    17281 cctccgctca cggcagcctg aaaatcatga tcccgatgat ctcctcaatg gaagagatct
    17341 tatgggtgaa agaaaaactg gcagaagcca aacagcaact acgtaacgaa cacattccgt
    17401 ttgatgagaa gatccagctc ggcattatgc tggaagtgcc gtcggtgatg ttcatcatcg
    17461 atcaatgctg cgaagagatt gatttcttta gtattggtag taatgacctg acgcaatatc
    17521 tgctggcagt ggatcgcgat aacgctaagg ttactcgtca ctacaacagc ctgaatccgg
    17581 cattcttgcg ggcgctcgat tacgacgtgc aggcggtgca tcgccagggt aaatggattg
    17641 gtctgtgcgg tgagctggga gcgaaaggtt ccgtgctgcc gttgctggtc ggcttagggc
    17701 tggatgaact cagcatgagc gcaccatcaa ttccggcggc gaaagcgcgg atggcgcaac
    17761 ttgatagccg tgagtgccgc aagttgctca accaggcaat ggcctgccgt acttcgctgg
    17821 aagtggaaca cctgctggcg caattccgca tgacccaaca ggacgcaccg ctggtcaccg
    17881 ccgagtgcat cacgctggaa agtgactggc gcagcaaaga agaagtgctc aaaggcatga
    17941 ccgataacct gctgctggcg ggccgctgcc gctatccgcg taaactggaa gccgacttat
    18001 gggcgcgcga ggccgttttc tctaccggtc tgggctttag ttttgccatt ccacacagca
    18061 aatcagaaca cattgagcaa tccaccatca gcgtggcgcg tctgcaagcg ccggtgcgct
    18121 ggggcgatga tgaagcgcaa ttcatcatta tgttaaccct gaacaaacac gctgcgggcg
    18181 atcagcacat gcgcattttc tcgcgcctcg ctcgccgcat catgcacgaa gaattccgta
    18241 acgcgctggt taacgccgcc tctgccgacg ctatcgccag cctgctgcaa catgaactgg
    18301 aactgtaaaa ggaaacatca tggaactgta tctggacacc gctaacgtcg cagaagtcga
    18361 acgtctggca cgcatattcc cgattgccgg ggtgacaact aacccgagca ttatcgctgc
    18421 cagcaaggag tccatctggg aagtgctgcc gcgtctgcaa aaagcgattg gtgatgaggg
    18481 cattctgttt gctcagacca tgagccgcga cgcgcagggg atggtggaag aagcgaagcg
    18541 cctgcgcgac gctattccgg gtattgtggt gaaaatcccg gtgacttccg aaggtctggc
    18601 agcaattaaa atactgaaaa aatagggtat tactacactt ggcaccgctg tatatagcgc
    18661 cgcagaaggg ttattagccg cactggcagg ggcaaaatac gttgctccgt atgttaaccg
    18721 cgtagatgcc cagggcgggg acggcattcg tacggttcag gagctgcaaa cgctgttaga
    18781 aatgcacgcg ccagaaagca tggtgctggc agccagcttt aaaacgccgc gtcaggcgct
    18841 ggactgttta ctggcaggat gtgaatccat caccctgccc ttagatgtag cgcaacaaat
    18901 gctcaacacc cctgcggtag agtcagctat agagaagttc gaacacgact ggaatgccgc
    18961 atttggcact actcatctct aaaggagcaa ttatggaccg cattattcaa tcaccgggta
    19021 aatacatcca gggcgctgat gtgattaatc gtctgggcga atacctgaag ccgctggcag
    19081 aacgctggtt agtggtgggt gacaaatttg ttttagtttt tgctcaatcc actgtcgaga
    19141 aaagctttaa agatgctgga ctggtagtag aaattacgcc gtttggcggt gaatgttcgc
    19201 aaaatgagat cgaccgtctg cgtggcatcg cggagactgc gcagtgtggc gcaattctcg
    19261 gtatcggtgg cggaaaaacc ctcgatactg ccaaagcact ggcacatttc atgggtgttc
    19321 cggtagcgat cgcaccgact atcgcctcga ccgatgcacc gtgcagcgca ttgtctgtta
    19381 tctacaccga tgagggtgag tttgaccgct atctgctgtt gccaaataac ccgaatatgg
    19441 tcattgtcga caccaaaatc gtcgcgggcg cacctgcacg tctgttagca gcgggtatcg
    19501 gcgatgcgct ggcaacctgg tttgaagcgc gtgcttgctc tcgtagcggc gcgaccacca
    19561 tggcgggcgg caagtgcacc caggcggcgc tggcactggc tgaactgtgc tacaacaccc
    19621 tgctggaaga aggcgaaaaa gcgatgcttg ccgccgaaca gcatgtagta accccggcgc
    19681 tggagcgcgt gattgaagcg aacacctatt tgagcggcgt tggttttgaa agcggcggct
    19741 ggcggcggct cacgcggtgc ataacggcct gaccgctatc ccggacgcgc atcactatta
    19801 tcacggtgaa aagtggcatt tggtacgctg acgcagctgg tactggaaaa cgcgccggtg
    19861 gaggaaatcg aaaccgtagc tgcacttagc catgcggtag gtttgccaat aactctcgct
    19921 caactggata ttaaagaaga tgtcccggcg aaaatgcgca ttgttgcaaa agcatcttgt
    19981 gcagaaggtg aaaccatcca caacatgcct ggcggcgcga cgccagatca ggtttacgcc
    20041 gcactgctgg tagctgacca gtacggtcag cgtttcctgc aagagtggga ataacctact
    20101 ccaaactccc ggcttgtccg ggagtttgaa cgcaaaattg cctgatgcgc tacgcttatc
    20161 aggcctacgc aatctctgca atatattgaa tttgcgtgct tttgtaggcc ggataaggcg
    20221 ttcactccgc atccggcact ctgtgccagt aatctgaaac tcccacgttg ttcaggagtt
    20281 ttttatccgc ttctggacat tttctccaca gaacaatcgt tagcccggaa ggtcgaatct
    20341 gtgactctta ttttcacgga cgaaccagat gaaagcttca ctggcgctgc tcagcctgct
    20401 aacggcattt accagccact ccttaaaatc tcccgccgtt ccaccaaccg tggtacaaat
    20461 ccaggccaat accaaccttg ccatcgcaga cggtgccaga cagcaaattg gcagcacgct
    20521 attttacgat cgcagcttac ctatcctggc ggtgatgttc cgcaagaacg cggtgtatgt
    20581 tccgatatgg tgatccgcgc attgcgcagc cagaaggtcg atttgcagaa actggttcac
    20641 gaagatatgg cgaagaattt tgctgagtac ccgcaaaaat ggaagttaaa gcgcccggac
    20701 agcaacatcg atcaccgtcg ggtgcctaat ctggaaacct ggtttagccg ccacaacaaa
    20761 acacgcccca tcagtaaaaa tgccggtgac tatcaagcgg gcgatattgt ctcctggcga
    20821 ttagataacg ggctggcgca tattggggtg gcgtcagatg gcttcgcccg cgacggtacg
    20881 ccgctggtga tacataacat tggcgcaggc gcgcaggaag aagatgtgct gtttagctgg
    20941 cggatggtgg ggcattatcg ttattttgtt aaataagaga tcgccccaca tgaagaaaaa
    21001 attcagatgc tttttacact gacagcttct tttttcttac ggctcaccag cgccagtgcc
    21061 agcacaatca gcacaatacc gctaccttca acggcccccg gattctcacc gagcagccac
    21121 caggaaaaga gtacgccaca aacgggaacg gccagtgtgc ttaagctggc aatactggca
    21181 ggcaagtttt tcagcacaaa cagccataag ctccacgcta gcgccgtcgc cagaatcgca
    21241 ctgtaggcca gcgcccagaa cacggtgggc tgccagtcaa tttcacgttg cggcaccagt
    21301 aaagcgacca cactcatcac cagcgccgca tacagcatct gccaggatgt taacgataat
    21361 aaatccacgc gcggatgacg ggcgtatagg cgtttagcaa caatcgcgct cgccccccaa
    21421 ctaacaccgg agaggattgc cagcatggca cttttcatcg aagagaaatc gagttgccac
    21481 ggctgcaaca ccaaaaataa gccgaaagcg gcaatcagaa tcgcgaaata ttgcccacgt
    21541 cgcaggcgtt caccgagaaa caacgcggcg aaaatcacca cccagaacgg catggtatag
    21601 ctcaggatcg ccaccttccc cgcacctccg ctgaccaacg cccactgcgc cagaccaacc
    21661 atcccgcagg tttgtaacag ggcaatggct aaggtgtatt taaacggcgt cgggcgcatt
    21721 ccgcgaccac gtaataaaag gacgatgaat aaaacgagag cgccgaaaat gcagcgtaag
    21781 gcggtaaagt cgaaggcacc gatgtaactg gtgacttgct tcatgaaaat ccagctataa
    21841 ctccagataa gtgtgagcac aaccaagcca ctgattgcca gtgggttgct ctttcctgcg
    21901 gcagacatga gagatccagt gtgtagtttt cctgtggaca ctatacgccg gtaaagaaac
    21961 ttcagcgact gcctgaaaga cagtcgctga agaaggtcag attacagcag gtcgaaacgg
    22021 tcgaggttca tcactttcac ccatgccgct acgaagtctt taacaaactt ctcgtgggca
    22081 tcgctgctgg cgtaaacttc cgctactgca cgcaggacag agttagaacc aaataccaga
    22141 tccgcacggc tggctgtgta tttcacttcg ccggtttcgc ggtcacgtcc ttcgaacagc
    22201 tctttcgatt cgtcagtcgc tttccactcg taacgcatat ccagcaagtt cacgaagaag
    22261 tcattgctca atacgccaac gcggtcagtg aagacgccgt ttttgctgcc atcgaagttg
    22321 gcacccagta cacgcatccc gcccaccagc gcagtcattt ccggcgcggt cagcgtcagt
    22381 tgctgtgctt tgtcgatcag cagtgactcg gtggtggaaa cgtccagacg agcgcgatag
    22441 ttacggaaac cgtcagcaat tggctccagc agctcaaaca tctcaatgtc agtctgatcc
    22501 tgacgcgcat caacgcgacc cggcgcaaac ggtacatgaa tgctcaaacc tgcggcgctt
    22561 gcggctttct caacaccaac cacaccagcc agcacgatga tatccgccag cgaggcttta
    22621 ccagactctt tctggatttt ctccagaaca ggcagagcac gaacggctgc ggcgttcaca
    22681 tcccagtcgc gctgcggcat taatgccaga cgcgcaccgt tggcaccacc gcgtttgtcg
    22741 ccaccacgga aggtagaagc agatgcccag gccaccgata ccagctcact aacagacaga
    22801 ccagaatccg caatcgcgaa tttcagatcg ataatgtcct gctcggtcgg gttgtagatc
    22861 ggctgcggca gcggatcttg ccagatcaga tcttctttcg gcacttccgg cccgatgtag
    22921 cgagatttcg gccccatatc cctgtgcgtc agtttgaacc aggcacgggc aaaggcttcg
    22981 ttgaacgcct gcggatcgtt gaggaaacga cgagagatct tctcgaactc aggatcaaaa
    23041 cgcagcgtca ggtcggtcac cagcattgtc ggtttacgtt tcttcgacgg atcgaacgga
    23101 tccgggataa tttccggtgc gtctaccgct tcgaactgga ttgctccagc cggactgcgg
    23161 gtctgtaccc actcatactt gaacagattc tcgaagaaat agttgctcca ctggatcggc
    23221 gtctgggtcc agactacttc cagaccagag gtaatggcat ctgcgccaac gccgctgccg
    23281 taagtgctcg cccagcctaa accttgttct tcaatcggtg cagcttctgg atcaggacct
    23341 acgtttgatg tcggaccggc accgtgggtt ttacccagcg tatgaccacc cgcaatcagc
    23401 gccacggttt cttcgtcgtt catgcccatg ttgccgaagg tcgcgcggat agctgctgcc
    23461 gcagaaagcg gttcgccgct gtgatccggg ccttccgggt taacgtaaat caggcccatc
    23521 tcggttgcac ccagcggtgc tttcgccagc gcttccggat gacggtgagt cagccaggct
    23581 ttttcatcac cccagttaac atccagatcc ggttcccaga cgtcttcacg accggcacca
    23641 aaaccgaagg tacggaagcc ggagttttcc agcgccacgt tacctgcgag gataaacagg
    23701 tcggcccagg agattttctg accatatttc tgtttgattg gccacaacag gcgacgcgct
    23761 ttatcgaggc ttacgttatc cggccaggag ttcagcggtg caaaacgttg ctgaccacga
    23821 cccgcgccgc cgcgtccatc gattgaacgg taagtacctg cgccgtgcca agccatacga
    23881 ataaacagac cggcgtagct gccccagtcg gctggccacc acggctgaga ttctgtcagc
    23941 agggctttca gatctttttt caggccgtag taatctaatt tgctgaattc tttgcggtag
    24001 tcaaagtcct cacccagcgg gttagaacga ttagaatgtt ggtttaacag gtcaacacgg
    24061 agttgatttg gccaccagtc gcgagtggtt gtgcccgccc ccgcgctctg gtcgtgaccg
    24121 ccctgatgga acgggcattt gccagtggct gtggtgttat ggatatcgtc tgacgtgctc
    24181 atcaatgtgc tcccctctac agtgttaccg ttacgataca cagcgttaga gagaagttat
    24241 aattgaatta atccacggat gcgatagttg agatcttaca tattgttggt taaagagatg
    24301 tagatcaaat tgatcgtaat taagcttttt tgattatgtc cgaattcgga caaatgcttt
    24361 ataaaaaagg gttttagctt catatccttc aagtaatgag ggagaaaaca aggctgacaa
    24421 ccttattttc ccgccctcat ttcgaggcag cattttgtgc tctgtttaaa atttgtgatc
    24481 actgtgtgat tttcacaaaa gccacactat ttataaacca ggtcgaacac ccagcgtatg
    24541 gcaaatcgcg taactcattt cagcgcggtt aagcgtatag aagtggaaat ctttcactcc
    24601 ttcacggctt aaaatcttca ccatatccat ggcaatattc gcgcccacca gtttgcgggt
    24661 ttcggcatcg tcatccagcc cgtcaaacat ttgcgccatc cacgccggaa tacgcacgtt
    24721 ggtcatgtcg gcaaatttct tcgcctgttt aaagttagat accggcaaaa tgcccgggat
    24781 gatttccaca tcaatgcccg ccgatacaca gcggtcacga aaacgcaggt agctttcgac
    24841 atcgaagaag aactgagtaa tcgcgcggtt ggctccggca tccactttgc gtttcagatt
    24901 aagcaaatcc gcctgagcgc tttttgcttc cgggtgaact tccggatacg ccgccacgga
    24961 tatatcgaaa tctgccactt cttttaacag cgtcaccagg tcagaagcat acatttctgg
    25021 cttaccactt cccggcggca gatcgccacg cagtgccacg atatgacgaa taccgttatt
    25081 ccagtagtcg cgtgcaatgg tgcgcagctc gtcgggcgtc gcatcaatgc aagtaagatg
    25141 cggtgccgct tccagaccag tgcgatcttt aatgccttta ataatgctgt gcgtacggtc
    25201 gcgctcgccg gagttcgcgc cataggtcac cgatacaaac ttcggtttca ggctgctaag
    25261 gcgatcgatg gagttccaca gggtctgctc catttcactg gtacgcggcg ggaaaaactc
    25321 gaacgaaacg ttaatctgcc cctggacttc tgccaggctc tgattcaggg catcccgctg
    25381 gctggcgtga aaaaagctca taccttacct catcaatcgc ttgtagttat atgttgtgtt
    25441 gtgcacatct atccatttag acgtccagat gtaaagatga aggaaaagcc gaagggcgtc
    25501 aaccgaaaaa tgaccaatga tggtgaggaa aaatcaggga agatgaaaaa acttcaggat
    25561 ggtaagaaaa agaaaatccg atatgcaaag caaaccggac tgcaaatata aactaaccat
    25621 gtacttattg cttcttgccg gattcgtcgt aaacgcttca tccggcctaa ttctatgata
    25681 attcaatata tttcattaga aaccatagac ccgaaaacat gagtaccggg cattattaaa
    25741 tttctgaaat tacaacaact gtgccagccg gttgatatcc gactgaatcg ccccggcggt
    25801 gacgtcgcgc ccagcgccag gcccacggat caccagcgga ttatcgcggt accagcggct
    25861 ttcgatggca aagacgttat cgcatggcag cagtgatgcc agcggatagt cttcacgcac
    25921 cgcctccacg cccacacgcg ctttgccgtt ggcatcgaaa cgcgccacgt agcgcagcac
    25981 cagccccatt tcgcgggccg cttccagccg ttgcaccatc tgctcgttaa gttcatcgcc
    26041 attttcaaag aaatggtcga tgctgccgcc ttcgcaatga gcaggcacca gcgattccac
    26101 acgtacctgg tccggttcaa tgttgtaacc cgcttcacgc gccagaatca ccagcttgcg
    26161 cattacgtct ttgccggaga ggtcgtcacg cggatcaggc tcggttaagc cctgctgcca
    26221 cgcctgatcc accagctcgg taaacggcac gctaccgtcg aattgcagga acagccagga
    26281 gagcgtgccg gagaagatcc cactgatcga caaaatggta tcgccgctgt cgatcagatc
    26341 gcgcacggta tggttgatcg gcaagcccgc accaacggtg gcgttgtata gccagtgacg
    26401 cccggttttt tcgaaggcgt cgtggatctg gcgatattta ttgctgtcgc ttgctcccgc
    26461 cagtttgttg gcactgatga cgtggaaacc gtggctggcg aaatcgagat actgatcagc
    26521 aagctgctgg ctggcggtaa cgtccaacac cactaaatca tcatacggat gggcgcgcat
    26581 ccacaggaaa agcgactctt catcctgctc gaccgcttca tcgttgaaga aggctaacgc
    26641 gcggctggcg tccagcccgt cataactcaa cagactgcgg cggctgtcca ccacgcctgc
    26701 cagcacaaat tcaaagccgg tacgcgccga aagcgtgctc tgctcgcggg cgaacagttc
    26761 cagccagcga gagccgatat tgcctttgcc gaacaatacc aggccgatgc gtttttctgc
    26821 gcggaagacg gactgatgca gcccctgaat caggctttcg gtcgggccgg tgcgcagtac
    26881 tgccaccaga ctgatgccgt catcggactg ccacgtaaac tcgaccggct ggcctttcag
    26941 ttgctgccag aagcggtggc aatgcagcgg gttacgggtg acacccgccc cgaccatcgc
    27001 caccagcgcc agcccctgac gcaggcgcag ttctccaggt aatcccgctt cgtcgaggat
    27061 tttcagcgca ctgtcggcca cttctgaggt gtagcaaaat tgcaacaact ggcgatcgtt
    27121 atgcacgcca accgccagtg gacgcacctg ggcacgtttc aggatctggt cgatctcttt
    27181 atgcgccagt ttgaaatcct gactggcggg cacctgaaac tcaatcaaac agacatcatc
    27241 gtggctggtg acaatacgcg caccagtacc ggaggccagc acgcgttcaa tgcgcgtgga
    27301 accttgatcc ggcgtgtagc tacagcgcag ctgcaggtcg atttcgctgc cagaaactgg
    27361 ctgtaaagta cgggcgtgaa gaacgggagc cgccagacgc gccagttcgc tggcctcatc
    27421 cagacgcagc aacggcagca ggcaggcatc tttcacttta cgcgggtcgg cactgtatac
    27481 cccggcgacg tcgctccaga tggttacacg agaaacaccc gccagcgcac cgatttgtgt
    27541 cgcggaatag tcggaaccgt tacgccccag caacaccgtt tcaccggcgt tgttacggct
    27601 gataaacccg gtcaccacca gacgtttgct cggatgttgt accagtagct gttgcagcaa
    27661 cgggtaagaa agcccttcat caacctgcgg ttgcgcagcg cgttcagcgc gtaaaaactc
    27721 acgggcatca agccaggccg ctggcagccc ttgttgatta agtaccgcag acatcaggcg
    27781 cgccgaccac acttcaccgt ggcccaccac ttccgcatac accgcatcgt taataccact
    27841 gtcgagcaac gccgccaggc gctcaagatc gctgacaaaa gcgctaatga ggctatcggc
    27901 ttcttcagcg ggtagcagac cgctaatcag atcgcactga tagcgacgca gcgtttgttg
    27961 aacctgatgc gcagagagac gatcggtctg gcttagtttc aaccagttaa tcaactggtt
    28021 agtggtgcta ccggcggcgg aaaccaccat catatcgtca ggctgagaga actccgccat
    28081 aatgcccgcg acacgcaaat aacacttcac atcagccaga ctactgccac caaatttatg
    28141 cagctgacga cctttcgccc ctgcctgcgc aatcacactc atttttaccc cttgtttgca
    28201 gcccggaagc cattttccag gtcggcaatt aaatcttcgc catcttcaat accggtggag
    28261 atacgcagca gcgtctcgga gatcccggcg gcagcacgcg cttctggtgc catgcctgca
    28321 tgtgtcatgg ttgcggcgtg agagattaaa ctttccactc cccctaatga ttccgccagc
    28381 gtaaacaacg acagcccgcc caggaaacga cacagcgtct gctcatcgcc atccagttca
    28441 aaactcaaca ttgcgccaaa gcctttttgc tgacgcgcgg caatttcatg cccctgattt
    28501 tccggcagcg acgggtgata cagttttttc accaacggct gggtttgcag atatttcaca
    28561 atcgcctgcg cgttgcgctg cgccagctcc atacgcggca ccagcgttcg caacccgcgt
    28621 agcagcagat agctgtcaaa cgcgccgccc gtcacgccaa tattgtttgc ccaccaggca
    28681 agttcagtga caacgtccgg gtctttagca atcaccacgc cagccaccac gtcagagtga
    28741 ccgttcagat atttcgtaca tgaatgcaac accagatcgg cacccaatgc cagcggattt
    28801 tgtaatgccg ggcttaagaa ggtgttatcc accacgctca ccgccccgac ttcccttgcc
    28861 agatggcaga ttttcgcaat atccacgacg cgtaacaatg gattacttgg gctttctacc
    28921 agtaccagtt tgggtttttc tgccagcgct gcccgtaatg cctgttcatc gccttgatca
    28981 acaaacaaca cgcgatagca accgcgtttc gccagactgt cgaacaggcg atagctaccg
    29041 ccgtagcagt cgtgcggcgc aaccagcaga tcgccaggtt tcaaaaagac ggtcgttacc
    29101 aggtgaatcg cggacatgcc ggtattagta agtactgcgc cagcaccgcc ttccagttct
    29161 gccagcgcac gctgaaccac atcgcgcgtt gggttgccgc gacgcgagta atcatgcgcg
    29221 cgcggttcat taaatccggt aaagttatag gtgctggaaa gatggatggg agggacaacg
    29281 caaccatact gttcgtcgtc atttaacccg ctacgcactg cgatggtggc ctgtttacgc
    29341 gtcatgtgat gaagtttcct gggctttgtc ggtgaaatgt caggcaccag agtaaacatt
    29401 gtgttaatgg acgtcaatac atctggacat ctaaacttct ttgcgtatag attgagcaaa
    29461 tcccaaatag ccgttaaaat tatatgcatt atcacgccga caggtgcatt acacgatgtc
    29521 acggtaacgc ctgtacggta aactatgcgg gtttacggtc agtacccaca taaattgtgg
    29581 ggtctggtct caatttattg acgaagagga ttaagtatct catggctgaa tggagcggcg
    29641 aatatatcag cccatacgct gagcacggca agaagagtga acaagtcaaa aagattacgg
    29701 tttccattcc tcttaaggtg ttaaaaatcc tcaccgatga acgcacgcgt cgtcaggtga
    29761 acaacctgcg tcacgctacc aacagcgagc tgctgtgcga agcgtttctg catgccttta
    29821 ccgggcaacc tttgccggat gatgccgatc tgcgtaaaga gcgcagcgac gaaatcccgg
    29881 aagcggcaaa agagatcatg cgtgagatgg ggattaaccc ggagacgtgg gaatactaaa
    29941 gcggaaaaga gcggcgcgga gtggaatcgc ctgatgcact acgcttatca ggcctacgtc
    30001 atattgcaat ttattgaatt tgcacgaact tgtaggccgg ataaggcgtt cacgccgcat
    30061 ccggcataaa caacgagcac gttgtctgcg acccaccgct ttttatacat ggacgtttaa
    30121 ctatgaaaaa caggctgctg atcctcagcc tgctggtttc tgtacctgcc tttgcctggc
    30181 agccgcaaac tggcgacatc atctttcaaa tctctcgctc atcgcaaagt aaagcgatcc
    30241 aactggcaac ccattccgat tacagccaca ccggtatgct ggtgatgcgt aacaaaaagc
    30301 cctacgtttt tgaagcggtc ggcccggtga aatacacccc gctcaagcag tggattgccc
    30361 atggtgaaaa gggcaactac gttgttcgcc gcgttgaagg cggactgagt gttgaacaac
    30421 agcaaaaact gacgcaaacg gcaaaacgtt atctcggtaa accgtatgat ttcagcttct
    30481 catggagcga cgatcgccag tactgttcgg aagtggtgtg gaaggtttac cagaacgcgc
    30541 tgggaatgcg cgtgggcgaa cagcagaagt tgaaagagtt tgatctcagc aacccgctgg
    30601 tccaggcgaa gctcaaagaa cgctacggta aaaatattcc gcttgaggaa acggtagttt
    30661 caccacaagc cgttttcgac gcgccacaac tcaccacggt tgccaaagag tggccgctgt
    30721 tttcgtggtg atacgaccaa aattcaggca caaaaaaagc gccgtgcggc gcttttttcg
    30781 gaaatccggt cttatttgct gcccgggatg ttgaaacgct tgttgaagcg gtcaacacgg
    30841 ccaccggtag caacatcacg ctgtttgcca gtgaagaacg ggtggcactt gctgcacacg
    30901 tcgaggttca ggtcatgacc aacggtggag cggattttta ttacgttacc gcaagagcag
    30961 ctagcagtaa tttcttcgta tttcgggtga atatcttttt tcatgggaaa acctcggttt
    31021 aaggccgcgt cgctcttcca gccctaacgc cagacaccac gcgatgttaa aagtatagct
    31081 tcaatacgat catttcgtac gaaggcgcga aatcatacag aaattaacca gcgtatgcaa
    31141 actgatccgc actcttctac ggcaatgtgt atactaaccc accgaatttc aagtcaggat
    31201 gatgctatgc ccgttgccca cgttgccttg cccgttccgc ttcctcgtac ctttgactat
    31261 ctgctgccag aaggcatgac ggttaaagct gggtgtcgcg tgcgcgtgcc gtttggcaaa
    31321 cagcaggagc gcatcgggat tgtggtatca gttagcgatg ccagcgaact gccgctcact
    31381 gagctaaaag cggtagtcga agtgctggat ggtgagccgg tgtttactca ctccgtctgg
    31441 cgattgctgc tatgggcggc agattactat catcatccga ttggcgatgt gctgtttcat
    31501 gccttgccga ttttactacg ccaggggcgg cctgcggcga acgcgccgat gtggtactgg
    31561 tttgccactg aacaaggtca ggcggtggat ctgaacagcc tgaaacgctc acccaagcaa
    31621 caacaggcgc tggcagcgtt acggcaaggc aaaatctggc gcgaccaggt tgccacgctc
    31681 gaatttaatg atgccgcgtt gcaggcgctg cgcaaaaaag gtctgtgtga tttagcaagt
    31741 gaaacaccag agtttagcga ctggcgaacg aactatgccg tttctggtga gcggttgcga
    31801 ttgaataccg aacaggccac cgccgttggc gcaattcata gcgcggcaga tactttttct
    31861 gcctggctgc tggcgggcgt taccggttcc ggtaaaacgg aggtttatct cagtgtactg
    31921 gaaaacgtgc tcgctcaggg caaacaggcg ctggtgatgg tgccggaaat cggcctgaca
    31981 ccgcaaacta tcgcccgttt tcgtgaacgg tttaatgcgc ccgtggaagt tctgcattcc
    32041 ggtctgaacg acagcgagcg tctttcggcg tggctgaaag cgaaaaatgg cgaggcggcg
    32101 attgtgatcg gcacccgctc cgcgctgttt acgccgttta aaaatctcgg cgtgattgtc
    32161 attgacgaag agcacgacag ttcctacaag cagcaggaag gctggcgcta tcatgcccgc
    32221 gacctggcgg tgtatcgtgc gcatagcgag caaatcccga ttattcttgg ctccgcaacg
    32281 cccgcgctgg aaacgttgtg caacgtccag cagaaaaaat accgcctgct gcgcctgacg
    32341 cgccgggcgg ggaatgcgcg tccggcaatt caacacgtgc tggatttaaa aggtcagaag
    32401 gtgcaggcag gtctggctcc ggcgttaatc acccgtatgc gccagcattt acaggccaac
    32461 aatcaggtta tcctctttct taaccgccgt ggctttgcgc ctgcgctact gtgccacgac
    32521 tgcggctgga ttgccgaatg cccacgttgc gatcactact acacgctgca tcaggcgcaa
    32581 cagcatttgc gctgtcacca ctgtgacagt cagcgtccgg tgccgcgcca gtgcccttcc
    32641 tgcggttcca cgcacctggt acccgtgggg ctgggcaccg aacagcttga acagacgctt
    32701 gcgccgctgt tccctgatgt gcccatttct cgtatcgacc gcgataccac cagccgcaaa
    32761 ggggcgctgg aacagcaact ggcggaagta catcgcggcg gtgcgaggat tttgatcggc
    32821 acacaaatgc tggcgaaagg tcaccacttt ccggatgtga cgctggtggc gttactggat
    32881 gtggacggcg cgctgttttc tgccgatttt cgctcggcag agcgtttcgc tcagctttac
    32941 acccaggtcg ctggtcgtgc cgggcgtgcg ggtaaacaag gcgaagtggt gctgcaaacg
    33001 caccatccgg aacatcctct gttgcaaacg ttgctctata aaggctacga tgcctttgcc
    33061 gaacaggcgc tggctgagcg acggatgatg caactgccgc catggaccag ccatgtgatt
    33121 gtgcgtgcgg aagatcataa caatcagcat gcgccgctgt tcctgcaaca actgcgtaat
    33181 ctgatcctct ccagcccgct ggcagacgac aaactgtggg ttctcggtcc ggttccggca
    33241 ctggcaccta aacgtggcgg tcgctggcgc tggcagatat tgttgcagca cccttcccgc
    33301 gtgcgcttgc aacacatcat tagtggtacg ctggcgctca tcaatacaat accggattcc
    33361 cgcaaggtga aatgggtact ggatgttgat ccgattgagg gttaaaccgc ttacgatgcg
    33421 aggcggatcg aaaaattcaa tattcatcac acttttcatg aaaattctgt aaccgttttc
    33481 acgcgctatc tgctaaaaat gttgccgatg tgaagtaaac atggatgtag tacgcctgac
    33541 gtgccaggcg aggagtgagt gtgaaagcga agaagcagga aactgccgcg accatgaaag
    33601 acgttgccct caaggcaaaa gtctctacag cgactgtctc ccgagcatta atgaatcccg
    33661 ataaagtctc ccaggccacc cgtaatcggg ttgaaaaagc ggcccgggaa gtgggttatt
    33721 taccgcagcc tatggggcgc aacgtcaagc gtaatgaatc ccgcaccatt ctggtgattg
    33781 tcccggatat ctgcgatccc ttctttagcg aaattattcg cggtatcgaa gttacggcgg
    33841 caaatcacgg atatctggtg ctgattggcg actgtgcgca tcaaaatcag caggaaaaaa
    33901 cctttatcga tttgatcatc accaagcaaa ttgatggcat gttgctgctg ggttcaaggc
    33961 tgccgtttga tgccagcatt gaggaacagc gtaatctgcc gccgatggtg atggcgaacg
    34021 aatttgcacc ggaactggag ctgcctaccg ttcatatcga caatctgacc gccgcatttg
    34081 atgcagtaaa ttatttatat gagcaagggc ataaacggat tggctgtata gccggtcccg
    34141 aagagatgcc actgtgtcac taccgcctgc aaggctatgt tcaggcgctg cgtcgctgcg
    34201 gaattatggt tgatccacaa tacatcgccc gtggcgactt caccttcgaa gccggaagca
    34261 aagcaatgca acagctgctt gatcttccac aaccgcctac tgcagtcttc tgccatagcg
    34321 atgtgatggc gctcggcgca ctttctcagg caaaacgcca ggggctgaaa gtcccggaag
    34381 acctttccat aatcggtttt gataacatcg acctgacgca attttgtgat ccgccgctga
    34441 caaccatcgc gcagccgcgt tacgaaatcg gtcgggaagc tatgctgtta ttgcttgatc
    34501 aaatgcaggg gcaacacgtt ggcagtggct ctcgtttaat ggactgcgaa cttatcatcc
    34561 ggggatcaac acgcgcgtta ccttaaagta aacctttaag actgccgtga ctggtcaaag
    34621 gcccgccgct taagtaacat ggcgggctga cgaacgaata aatacagcga aacgatagtg
    34681 gcacaacgag attatgtacg ccgcagccaa ccggcacctt cgcggcgaaa aaagagcacc
    34741 tcacggaaaa agcaacgaaa tctgcctgcg gtttctcccg ctatggtcgc tattgctgcc
    34801 gccgttcttg tgacctttat cggtggtctg tacttcatta cgcatcacaa gaaagaagag
    34861 tccgagacgc tgcaaagcca gaaagtgacc ggaaacggac taccaccaaa accagaagaa
    34921 cgctggcgct acattaaaga gctggaaagt cgccagccgg gtgtgcgtgc gcccacagaa
    34981 ccttctgccg gtggtgaagt gaaaacgccg gagcaactga caccagaaca acgtcagctt
    35041 cttgaacaaa tgcaggctga tatgcgccag cagccaacgc agctggttga ggttccgtgg
    35101 aatgaacaga cgccggaaca gcgtcagcaa acgctacaac gccagcgtca ggcgcagcaa
    35161 ttagcggagc agcaacgcct ggctcagcag tcgcgtacga ctgaacaaag ctggcagcag
    35221 cagacgcgta cgtcgcaagc cgcgccggta caggcgcaac cgcgccagtc taaaccagct
    35281 tctactcagc agccgtacca ggatctgctg caaacccctg cgcacacgac cgcgcagtcg
    35341 aagccgcagc aagctgcgcc ggttgctcgt gccgctgacg cgccaaaacc gacggcggag
    35401 aaaaaagacg aacgccgctg gatggtgcag tgcggttcgt tcagaggcgc ggaacaggca
    35461 gagacggtac gtgctcagct ggcgttcgaa ggatttgact cgaaaatcac caccaacaat
    35521 ggctggaatc gtgtggtcat tggcccggtg aaaggcaaag agaacgcaga cagcaccctc
    35581 aatcggttga agatggcggg tcatacaaac tgcattcggc tcgccgccgg gggttgaaac
    35641 cctcaaaatc ccccccatct ataattgcat tatgccccgt acttttgtac ggggtttgta
    35701 ctctgtattc gtaaccaagg ggtcagctcg tgacaactat agtaagcgta cgccgtaacg
    35761 gccatgtggt catcgctggt gatggtcagg ccacgttggg caataccgta atgaaaggca
    35821 acgtgaaaaa ggtccgccgt ctgtacaacg acaaagtcat cgcgggcttt gcgggcggta
    35881 ctgcggatgc ttttacgctg ttcgaactgt ttgaacgtaa actggaaatg catcagggcc
    35941 atctggttaa agccgccgtt gagctggcaa aagactggcg taccgatcgc atgctgcgca
    36001 aacttgaagc actgctggca gtcgcggatg aaactgcatc gcttatcatc accggtaacg
    36061 gtgacgtggt gcagccagaa aacgatctta ttgctatcgg ctccggcggc ccttacgccc
    36121 aggctgcggc gcgcgcgctg ttagaaaaca ctgaacttag cgcccgtgaa attgctgaaa
    36181 aggcgttgga tattgcaggc gacatttgca tctataccaa ccatttccac accatcgaag
    36241 aattaagcta caaagcgtaa ggatctccca tgtctgaaat gaccccacgc gaaatcgtca
    36301 gcgaactgga taagcacatc atcggccagg acaacgccaa gcgttctgtg gcgattgctc
    36361 tgcgtaaccg ctggcgtcgc atgcagctca acgaagagct gcgccatgaa gtgaccccga
    36421 aaaatatcct gatgatcggc ccgaccggtg tcggtaaaac tgaaatcgcc cgtcgtctgg
    36481 ctaagctggc gaatgcgccg ttcatcaaag ttgaagcgac caaatttacc gaagtgggct
    36541 acgtcggtaa ggaagttgat tctattattc gcgatctgac cgatgccgcc gtgaaaatgg
    36601 tacgcgtcca ggctatcgag aaaaaccgtt atcgcgctga agaactggca gaagaacgta
    36661 ttctcgacgt gctgatccca cctgctaaaa acaactgggg acagactgaa cagcagcagg
    36721 aaccttccgc tgctcgtcag gcattccgca aaaaactgcg tgaaggccag cttgatgaca
    36781 aagaaatcga gatcgatctt gccgcagcac cgatgggcgt tgaaattatg gctcctccgg
    36841 gcatggaaga gatgaccagc cagctgcagt ccatgttcca gaacctgggc ggccagaagc
    36901 aaaaagcgcg taagctgaaa atcaaagacg ccatgaagct gctgattgaa gaagaagcgg
    36961 cgaaactggt gaacccggaa gagctgaagc aagacgctat cgacgctgtt gagcagcacg
    37021 ggatcgtgtt tatcgacgaa atcgacaaaa tctgtaagcg cggcgagtct tccggtccgg
    37081 atgtttctcg tgaaggcgtt cagcgtgacc tgctgccgct ggtagaaggt tgcaccgttt
    37141 ccaccaaaca cgggatggtc aaaactgacc acattctgtt tatcgcttct ggcgcgttcc
    37201 agattgcgaa accgtctgac ctgatcccgg aactgcaagg tcggctgcca atccgcgttg
    37261 aactgcaggc actgaccacc agcgacttcg agcgtattct gaccgagccg aatgcctcta
    37321 tcaccgtgca gtacaaagca ctgatggcga ctgaaggcgt aaatatcgag tttaccgact
    37381 ccggtattaa acgcatcgcg gaagcggcat ggcaggtgaa cgaatctacc gaaaacatcg
    37441 gtgctcgtcg tttacacact gttctggagc gtttaatgga agagatttcc tacgacgcca
    37501 gcgatttaag cggtcaaaat atcactattg acgcagatta tgtgagcaaa catctggatg
    37561 cgttggtggc agatgaagat ctgagccgtt ttatcctata atcgcgttca atcattttca
    37621 tcattgtttg atggggctga aaggccccat ttttattggc gcgtattatg actgaacaac
    37681 aaattagccg aactcaggcg tggctggaaa gtttacgacc taaaaccctc cccctcgcct
    37741 ttgctgcaat tatcgtcggg acggcgctgg catggtggca aggtcacttc gatccgctgg
    37801 tcgccctgct ggcactaatt accgccgggc tattacagat cctttctaac ctcgccaatg
    37861 attacggcga tgcggtaaaa ggcagcgata aacctgaccg cattgggccg ctacgcggca
    37921 tgcaaaaagg ggtcattacc cagcaaaaga tgaaacgggc gctcattatt accgtcgtgc
    37981 tcatctgtct ctccgggctg gcactggttg cagtggcatg ccatacgctg gccgattttg
    38041 tcggtttcct gattcttggc gggttgtcga tcattgccgc tatcacctac accgtgggca
    38101 atcgtcctta tggttatatc ggtctgggtg atatttccgt actggttttc tttggctggt
    38161 tgagtgtcat ggggagctgg tatttacagg ctcatacatt gattccggca ctgatccttc
    38221 cggcgaccgc atgcggcctg ctggcaacgg cagtactgaa tattaataac ctgcgtgata
    38281 tcaatagcga ccgcgaaaat ggcaaaaaca cgctggtggt gcgcttaggt gaagtgaacg
    38341 cgcgtcgtta tcatgcctgc ctgctgatgg gctcgctggt gtgtctggcg ctgtttaatc
    38401 tcttttcgct gcatagcctg tggggctggc tgttcctgct ggcggcacca ttactggtga
    38461 agcaagcccg ttatgtgatg cgggaaatgg acccggtggc gatgcgacca atgctggaac
    38521 gtactgtcaa gggagcgtta ctgactaacc tgctgtttgt tttagggata ttcctaagcc
    38581 agtgggcagc ataactgaca aatatcaatt aacaattgat gattttgcca acagcccaca
    38641 tagcgcgata tactgaaaat tctcgcagca actgaatgtt aagcctatga aatacgatac
    38701 ttccgagctt tgtgacatct atcaagaaga tgttaacgtc gtggaaccgc tgttctccaa
    38761 ctttggcgga cgggcgtcgt ttggcggaca aataatcacg gtaaaatgtt tcgaggacaa
    38821 cgggttgctg tacgatctgc tcgaacagaa tggccgtggt cgtgttcttg tcgtcgatgg
    38881 cggtggttct gttcgtcgcg cactggtcga tgctgaactg gcgcgtctgg cagtacaaaa
    38941 tgaatgggaa ggtctggtca tttacggcgc ggtgcgtcag gtagatgacc tggaagagtt
    39001 ggatatcggc atccaggcga tggcggcaat tccggttggt gccgctggcg aaggcattgg
    39061 cgaaagcgat gtccgcgtca attttggcgg tgtcaccttc ttctccggcg accatcttta
    39121 tgccgacaat accgggatta ttctttcaga agatccgctg gatattgaat gataagaaag
    39181 gcaccgcaag gtgccttttt tctgcgttac ctgttggcct acacagtaaa gaaattacgc
    39241 gaaagatgaa gcgtaatcag acctcttcca tgcgacccag cagggcctgc agacgttcct
    39301 gccagccgtt ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat
    39361 gctgggcatt ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt
    39421 ccatctgcaa cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa
    39481 acacttctaa tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaaat
    39541 cctcgtcccg attaccggtg acgccttaat aaatacgagc gcactttagt tagctccgat
    39601 tgtatgaagc cgcgccatcg ctgtccagcg gcacgccttg cagattacgg tttgccacac
    39661 ttttcatcct tctcctagtg acataatcca catcaatcga aaatgttaat aaatttgttg
    39721 cgcgaatgat ctaacaaaca tgcatcatgt acaatcagat ggaataaatg gcgcgataac
    39781 gctcatttta tgacgaagca cacacatttt aagttcgata tttctcgttt ttgctcgtta
    39841 acgataagtt aacagcatgc ctacaagcat cgtggaggtc cgtgactttc acgcatacaa
    39901 caaacattaa ctcttcagga tccgattatg agtcaaacat caaccttgaa aggccagtgc
    39961 attgctgaat tcctcggtac cgggttgttg atttttttcg gtgtgggttg cgttgcagca
    40021 ctaaaagtcg ctggtgcgtc ttttggtcag tgggaaatca gtgtcatttg gggactgggg
    40081 gtggcaatgg ccatctacct gaccgcaggg gtttccggcg cgcatcttaa tcccgctgtt
    40141 accattgcat tgtggctgtt tgcctgtttc gacaagcgca aagttattcc ttttatcgtt
    40201 tcacaagttg ccggcgcttt ctgcgctgcg gctttagttt acgggcttta ctacaattta
    40261 tttttcgact tcgagcagac tcatcacatt gttcgcggca gcgttgaaag tgttgatctg
    40321 gctggcactt tctctactta ccctaatcct catatcaatt ttgtgcaggc tttcgcagtt
    40381 gagatggtga ttaccgctat tctgatgggg ctgatcctgg cgttaacgga cgatggcaac
    40441 ggtgtaccac gcggcccttt ggctcccttg ctgattggtc tactgattgc ggtcattggc
    40501 gcatctatgg gcccattgac aggttttgcc atgaacccag cgcgtgactt cggtccgaaa
    40561 gtctttgcct agctggcggg ctggggcaat gtcgccttta ccggcggcag agacattcct
    40621 tacttcctgg tgccgctttt cagccctatc gttggcgcga ttgtaggtgc atttgcctac
    40681 cgcaaactga ttggtcgcca tttgccttgc gatatctgtg ttgtggaaga aaaggaaacc
    40741 acaactcctt cagaacaaaa agcttcgctg taatatgact acgggacaat taaacatgac
    40801 tgaaaaaaat atatcgttgc gctcgaccag ggcaccacca gctcccgcgc ggtcgtaatg
    40861 gatcacgatg ccaatatcat tagcgtgtcg cagcgcgaat ttgagcaaat ctacccaaaa
    40921 ccaggttggg tagaacacga cccaatggaa atctgggcca cccaaagctc cacgctggta
    40981 gaagtgctgg cgaaagccga tatcagttcc gatcaaattg cagctatcgg tattacgaac
    41041 cagcgtgaaa ccactattgt ctgggaaaaa gaaaccggca agcctatcta taacgccatt
    41101 gtctggcagt gccgtcgtac cgcagaaatc tgcgagcatt taaaacgtga cggtttagaa
    41161 gattatatcc gcagcaatac cggtctggtg attgacccgt acttttctgg caccaaagtg
    41221 aagtggatcc tcgaccatgt ggaaggctct cacgagcgtg cacgtcgtgg tgaattgctg
    41281 tttggtacgg ttgatacgtg gcttatctgg aaaatgactc agggccgtgt ccatgtgacc
    41341 gattacacca acgcctctcg taccatgttg ttcaacatcc ataccctgga ctgggacgac
    41401 aaaatgctgg aagtgctgga tattccgcgc gagatgctgc cagaagtgcg tcgttcttcc
    41461 gaagtatacg gtcagactaa cattggcggc aaaggcggca cgcgtattcc aatctccggg
    41521 atcgccggtg accagcaggc cgcgctgttt ggtcagttgt gcgtgaaaga agggatggcg
    41581 aagaacacct atggcactgg ctgctttatg ctgatgaaca ctggcgagaa agcggtgaaa
    41641 tcagaaaacg gcctgctgac caccatcgcc tgcggcccga ctggcgaagt gaactatgcg
    41701 ttggaaggtg cggtgtttat ggcaggcgca tccattcagt ggctgcgcga tgagatgaag
    41761 ttgattaacg acgcctacga ttccgaatat ttcgccacca aagtgcaaaa caccaatggt
    41821 gtgtatgtgg ttccggcatt taccgggctg ggtgcgccgt actgggaccc gtatgcgcgc
    41881 ggggcgattt tcggtctgac tcgtggggtg aacgctaacc acattatacg cgcgacgctg
    41941 gagtctattg cttatcagac gcgtgacgtg ctggaagcga tgcaggccga ctctggtatc
    42001 cgtctgcacg ccctgcgcgt ggatggtggc gcagtagcaa acaatttcct gatgcagttc
    42061 cagtccgata ttctcggcac ccgcgttgag cgcccggaag tgcgcgaagt caccgcattg
    42121 ggtgcggcct atctcgcagg cctggcggtt ggcttctggc agaacctcga cgagctgcaa
    42181 gagaaagcgg tgattgagcg cgagttccgt ccaggcatcg aaaccactga gcgtaattac
    42241 cgttacgcag gctggaaaaa agcggtcaaa cgcgcgatgg cgtgggaaga acacgacgag
    42301 taatgtcagg cacctgaatc gattgcctga tgctgatcgc ctgatgcgac gcttacgcgt
    42361 cttatcaggc ctacgagcca atgccatttt gtgtaggcgg gataaggcgt tcacgccgca
    42421 tccggcatca cgcagactct gaaaccgtgc gggggcaacc ccgcacacat caataatccc
    42481 acccttcccc tgtgctacac ttcgcgccat tccttactgc ttagagtttg ctatgagacg
    42541 agaacttgcc atcgaatttt cccgcgtcac cgaatctgcg gcgctggctg gctacaaatg
    42601 gttaggacgc ggcgataaaa acaccgcgga cggcgcggcg gtaaacgcca tgcgtattat
    42661 gctcaaccag gtcaacattg acggcaccat cgtcattggt gaaggtgaaa tcgacgaagc
    42721 accgatgctc tacattggtg aaaaagtcgg tactggtcgc ggcgacgcgg tagatattgc
    42781 tgttgatccg attgaaggca cgcgcatgac ggcgatgggc caggctaacg cgctggcggt
    42841 gctggcagta ggcgataaag gctgcttcct caatgcgccg gatatgtata tggagaagct
    42901 gattgtcggg ccgggagcca aaggcaccat tgatctgaac ctgccgctgg cggataacct
    42961 gcgcaatgta gcggcggcgc tcggcaaacc gttgagcgaa ctgacggtaa cgattctggc
    43021 taaaccacgc cacgatgccg ttatcgctga aatgcagcaa ctcggcgtac gcgtatttgc
    43081 tattccggac ggcgacgttg cggcctcaat tctcacctgt atgccagaca gcgaagttga
    43141 cgtgctgtac ggtattggtg gcgcgccgga aggcgtggtt tctgcggcgg tgatccgcgc
    43201 attagatggc gacatgaacg gtcgtctgct ggcgcgtcat gacgtcaaag gcgacaacga
    43261 agagaatcgt cgcattggcg agcaggagct ggcacgctgc aaagcgatgg gcatcgaagc
    43321 cggtaaagta ttgcgtctgg gcgatatggc gcgcagcgat aacgtcatct tctctgccac
    43381 cggtattacc aaaggcgatc tgctggaagg tattagccgc aaaggcaata tcgcgactac
    43441 cgaaacgctg ctgatccgcg gcaagtcacg caccattcgc cgcattcagt ccatccacta
    43501 tctggatcgc aaagacccgg aaatgcaggt gcacattctc tgagtgattt gatcgattga
    43561 gccttccagt ccttcgggac tggaattttt ttgttcggag aacgaagata aggcaagtca
    43621 atcaaaacag gagaaaaaca tggctgattg ggtaacaggc aaagtcacta aagtgcagaa
    43681 ctggaccgac gccctgttta gtctcaccgt tcacgccccc gtgcttccgt ttaccgccgg
    43741 gcaatttacc aagcttggcc ttgaaatcga cggcgaacgc gtccagcgcg cctactccta
    43801 tgttaactcg cccgataatc ccgatctgga gttttacctg gtcaccgtcc ccgatggcaa
    43861 attaagccca cgattggcgg cactgaaacc gggcgatgaa gtgcaggtgg ttagcgaagc
    43921 agctggcttc tttgttctgg atgaagtacc ggactgcgaa acgctatgga tgctggcaac
    43981 cggtacagcg attggccctt atttatcgat tctgcaacta ggcaaagatt tagatcgctt
    44041 caaaaatctg gtcctggttc acgccgcacg ttatgccgcc gacttaagct atttgccact
    44101 gatgcaagaa ctggaaaaac gctacgaagg caaactgcgc attcagacgg tggtcagtcg
    44161 ggaaacggca gcggggtcgc tcaccggacg gataccggca ttaattgaaa gtggggaact
    44221 ggaaagcgcg attggcctgc cgatgaataa agaaaccagc catgtgatgc tgtgcggcaa
    44281 tccacagatg gtgcgcgata cacaacagtt gctgaaagag acccggcaga tgacgaaaca
    44341 tttacgtcgc cgaccgggcc atatgacagc ggagcattac tggtaagcgg ttacttatcg
    44401 ataaacggca cgatgagcaa atccgcactc atcttattga tcatcccgcg atatgccggc
    44461 atcaaacggt tgataaatga gtgatgatga ccacagacaa ggaggtcgca ctgctctttt
    44521 tgcataattt ccagcagtgt ttccggcatt tctccgcgtt caatacgcag ttttgtcttc
    44581 ggccattgaa tatttttcgt cagtttatac agcttgttat ccgacttatt cttcaacaat
    44641 tgaagaatat cttctgttgc agggaagtag atacccggat acaactcgct taagccatca
    44701 tcaatatgaa ttaacgtcag gtgagcgtca ttatgtctgg cgagctccag ggctttattc
    44761 accagtaagg catcttcttc attcccggaa attgccacgc caatgtgttt ataagccatg
    44821 tttaactcct tctaaagcaa ctccatcaag ctaacgaacg cagtgataac tcaaaaataa
    44881 tcatctcggc ctggcatgag aaagtgaagg ccgcatcaag ctcaacttgc ccctccagat
    44941 ccttaatctt ataggccacg ctggcccctt catctgcaga ggatgcagcg gcggcaagct
    45001 ctttcagttt ggtcataaaa gactctgctt cttcgcgggt ggcaaaaaag cgcgaaaact
    45061 tactggtaca gttgtcgtta tcaatcaccg taccgatatc aatcgcacat cctttagtac
    45121 tgcatttatc tacgacatct ttcatagggc acctctgtat attcgcctct ctgtcgatta
    45181 cgacttaaga ataatgcgcc gttaagggaa gaacaaacaa gtaatgatct tatggattac
    45241 catttttttt gatattgctt aaggtgaaga gttcagaagg attttccggg tattgtcatt
    45301 tgcatcgtgg aaataacaat cccggaaaaa aatagcagta agagatatat taaaggttaa
    45361 ttacttgatt tattgtcggc tttatacttc acatcctgag tatctttacc atatttattt
    45421 tcgccttgtg tgccaacaaa cgcaccaaga tcgataagca tcatcaccag aatcaacgtc
    45481 gggacaaaac gccccaccgc ccattgccag acacccggta aaatcgctca gttacccgcc
    45541 agcagcatcc acgccacaat catcagaaat gcccatgcgc cggaacgccc gcgatcatgc
    45601 aagcgcttaa cagttactgc cgctgttggc cagagcaagc acacaaggca aaacgccgcg
    45661 gtctgaatat cgagtagatt cttacccgcc agtgagaaaa gcaccagcat gcctgcgaac
    45721 cacaggccta tccaaatcca gaaatcacgg cgtccaatac gccctttaaa tgagaataac
    45781 cattgctgta tggtcatgta agttccttga tggttgtctt ttccaggatt ctaccctttt
    45841 gacaagggcg acagatatcg ttttaatcgg agccagtcat aacaaaaggt actgtcaatg
    45901 aagccagggt gtacgctgtt ttttctctta tgttctgcat taaccgttac aacaacggcg
    45961 catgcacaaa caccagatac ggcaacgacc gcgccttatc tgctggctgg agcccctact
    46021 ttcgatctct ccatcagcca gtttcgagaa gactttaaca gccagaatcc cagcctgcca
    46081 ctgaacgaat ttcgtgccat cgacagcagt cccgacaaag ccaatctcac tcgtgctgca
    46141 agtaaaatta acgagaactt gtatgcttct acagcgctgg agcgcggtac cttaaaaatc
    46201 aaaagcattc aaatgacctg gctacccatc caggggccag agcaaaaagc agcgaaagcg
    46261 aaagctcagg aatacatggc agcagtgatc cgcacactca ccccattaat gaccaaaaca
    46321 caaagccaga aaaaactgca gtcgctacta acggcgggga aaaacaaacg ttattacacc
    46381 gagacagaag gtgcactgcg ttatgttgtc gcggacaacg gcgaaaaggg gctgaccttc
    46441 gctgttgaac cgattaagct ggcgctatct gaatcgcttg aaggtttgaa taaatgacaa
    46501 aaagcaaagc ctttgtgccg atgaatctct atactgtttc acagacctgc tgccctgcgg
    46561 ggcggccatc ttcctttatt cgcttataag cgtggagaat taaaatgcga catcctttag
    46621 tgatgggtaa ctggaaactg aacggcagcc gccacatggt tcacgagctg gtttctaacc
    46681 tgcgtaaaga gctggcaggt gttgctggct gtgcggttgc aatcgcacca ccggaaatgt
    46741 acatcgatat ggcgaagcgc gaagctgaag gcagccacat catgctgggt gcgcaaaacg
    46801 tggacctgaa cctgtccggc gcattcaccg gtgaaacttc tgccgctatg ctgaaagaca
    46861 tcggcgctca gtacatcatc atcggccact ctgaacgtcg tacttaccac aaagagtctg
    46921 acgaactgat tgcgaaaaaa ttcgcggtgc tgaaagagca gggtctgact ccggttctgt
    46981 gcatcggtga aaccgaagct gaaaacgaag cgggaaaaac tgaagaagtt tgcgcacgtc
    47041 agatcgacgc ggtactgaaa actcagggtg ctgcggcatt cgaaggtgcg gttatcgctt
    47101 acgaacctgt atgggcaatc ggtactggca aatctgcaac tccggctcag gcacaggctg
    47161 ttcacaaatt catccgtgac cacatcgcta aagttgacgc taacatcgct gaacaagtga
    47221 tcattcagta cggcggctct gtaaacgcgt ctaacgctgc agaactgttt gctcagccag
    47281 acatcgacgg cgcgctggtt ggcggtgctt ctctgaaagc tgacgctttc gcagtgatcg
    47341 ttaaagctgc agaagcggct aaacaggctt aagtctgaca ggtgccggat ttcatatccg
    47401 gcacttactt tccttaactc ctcgccttaa cgcaaaatct cacactcatg atcctggatt
    47461 tcctcggctg aagcacggtt cagcgtcagt agatttcgtt gtgtcgccag caacacaaac
    47521 gaattatcac tctgccgtac catcgccagc ccgtagcttc ccatatgttc ccgcgcctca
    47581 ggcacttctt ctgcaagcat cataaatggg ctgcgttgta ccagttcgct ttccgttacc
    47641 cgacgcgcca ggtattcatg cccgcgcaaa ccacctggca gtggcaacca gcggctgctg
    47701 atgttcgcca gatttttatc cagttgttcg cgcacatcag gacgaataca agagatatga
    47761 atatgaaaat ggttttgcgt atgcccggtg cgggagttga tcgccaaaga aaccgcgcga
    47821 tcgggaaccg gctggccgta ttttttgctc ataaaatcac gcgcctgcca ggccaaccaa
    47881 aagaagttcg gcgttgaagg atcggtcaac aaaggacttt cagtaccgtt aatacgatac
    47941 gttggcatca acagatattg cagtggacca ttaagatctt ttaaaaccac gtatccggca
    48001 ttgggtttga cttccgcaca tggcgaagga ttttgatttt gctgctgatt gggcaaacat
    48061 tcctcaagga caatcttacg taatgtatcc gactcttcac cggttaattt ccagtaacca
    48121 ataccggcag ccacaacggc gataactatc atcaccaaaa aaagaagacc cgcttttttc
    48181 atcttttttt ccctgtacct caaagagatg caagggtaac gcaaaatcgt gacaaataaa
    48241 aaacccggtc gcgagaaacc accgggtttg ataattatcc tagggaaatc agcgtttgct
    48301 gatctgatcg aacgtaccgc cgttagcaaa atgctctttt tgcgctttcg tccagccgcc
    48361 gaactcttca tcaatggtga ataacttcag ctttggaaac gcattttcgt actttttcgc
    48421 tacttcagcg tcgcgcggac ggtagtagtt tttcgcggca atttcctgac cttctggcga
    48481 gtagagatat ttcaggtagg cttccgccac ctctttggtg ccttttttct cgaccacttt
    48541 atcgaccacc gacaccgttg gctctgcgag gatagactca ctcggcgtga cgatttcgaa
    48601 tttatctttc cccagttcat tcgctgccag cagagcttcg ttttcccagg cgatcagtac
    48661 atcgccaatc ccgcgctcga caaaggtgtt agtggagccg cgcgcgccag aatccagaac
    48721 ttcgacgttt ttatacagtg cccgaacaaa atcctgtgct tttgcctgat cgttgttgtt
    48781 gtgatgcagc gcgtagcccc aggcagccag gtagttccag cgtgcgccac cagagctttt
    48841 cggattaggc gtgatcaccg aaacacccgg tttaatcaga tcgttccagt catggatctg
    48901 cttcggattg cccttacgca ccaggaaaac aatggtggaa gtgtacggtg cggagttatc
    48961 cggcagacgt ttgatccact ctttatcaat ccgcccgcgt tccgcaatcg cgtccacgtc
    49021 ataggccaga gccagcgtga caacatcagc ttcaataccg ttgattaccg acgtcgcttg
    49081 tttacctgaa ccaccgtgtg actgacgaat caccacgtta tcaccagttt gctgtttcca
    49141 gtgggcgctg aatgccttgt tgtactgttc gtacaattcg cgcgttggat catatgaaac
    49201 gttaagaagc tgaatatcct ttgccataac gctggttgcc gccagcaaaa atgttaaccc
    49261 tacgccccac ttgttcatcg cccgactctc ttatgttgtg ttgtgatgag caaagcgtgc
    49321 cagaaggtta accaaacatt aaagaataaa aaaagattgg ctataacttg cgggtatatg
    49381 ttgagggatt aaaaaggcgg aagaaatccg cctcatattg ctgacaaagt gcgctttgtc
    49441 catgccggat gcggcgcgaa cgccttatcc ggcctacaaa agtttgcaaa ttcaataaat
    49501 tgcagaattc atgtaggcct gataagcgaa gcgcatcagg catttttgct tctgtcatcg
    49561 gtttcaggct aaaggaatct gcctttttcc gaaatcatta atacagtttt ttcgcgcagt
    49621 ccagccagtc acctttgaac ggacgcttca tgttttcgat agcgtcgatg atgtcgtggt
    49681 gaaccagctg ttcgttctgg atacctacac aacgaccgcc gtaacctgcc agcagcagat
    49741 cgatagcgta agcgcccata cgggaagcca gaatacggtc gtaaggcacc ggagaaccac
    49801 cgcgctggat gtggcccagc acagttgcgc gggtttcacg accggtttct ttctcgatga
    49861 aatgcgccag ttcgtcaaca tcacacatat gttcggtaat cgccacgatc gcgtgttttt
    49921 tacctttcgc gatacccgct ttgatttcgt ttaccaggtc ttcacggctg aattcaactt
    49981 ccggaactac aacgaattca cagccaccgg caatggccgc agccagggtc agatcgccac
    50041 aataacggcc catcacttcc accacggaaa tacgctggtg agaagaagag gtgtcacgca
    50101 gacggtcgat cgcttctaca acggtgctca gcgcagtgaa gaaaccgata gtgtagtcag
    50161 tgcctttgat gtcgttgtcg atagtgcccg gcagaccgat gcacgggaag cccatttcgg
    50221 tcagacgcat tgcacccatg taggaaccgt caccgccgat aaccaccagc gcgtcgatac
    50281 cacgtttttt caggttttcg atagccacgg cgcggatgtt ctcgtcgcgg aattccggga
    50341 aacgcgcaga accgaggaac gtaccgccac ggttgatcat gtcagacacg ctgtaacggt
    50401 ctagctgtac catacggtct tcatacagac ccagatagcc gtcataaata cccattactt
    50461 ccagaccttc tgtcagcgca gaacgaacaa ccccgcgaat tgcggcgttc atgcctggcg
    50521 catcaccgcc gcttgtcaac acacctattt tcttaatcat gactacctct gaactttgga
    50581 atgcaaaatg aaatctgttg ccggaagtct tcttgcacat cgaagtgatc caacgaatgt
    50641 gcaaatagta taacaatcac ttcctgctga attgattcag gtcaggccaa atggcggtat
    50701 tttatacaca aaatgcgggt ctggctctct tttatactga ttatgaaagc atagaccgtt
    50761 taccctccct gggtacgacg gaacaggggt cctgatggat aatcacatcc gatcccggaa
    50821 aacgccgtaa aatagcctgc tctacctgat ccgccaccat atgtgcctga accaaaggca
    50881 gagagtcttc catttccaaa tgaatctgaa taaagcgggt cggccctgac tgccgcgtgc
    50941 gaagatcgtg agcgccgcta acacccggcc aggaagtcac gatatcaata atttcttgcc
    51001 gttcttcatc aggcaatgcg cgatccagta atgactgtac cgcctcatat cccatgcgta
    51061 acgcgctata taaaatatag atgccgattc ccaatgcaaa cagagcatcg gcgcgatgcc
    51121 agccgtacca agacaacccc agcgccagca gaattgcgcc gttcatcata acatcagact
    51181 ggtaatgtag catatcagcc cgcaccgcct ggctttgcgt ccggcgcacc acccaccgct
    51241 gaaacgagac aaggataatc gtacaaatta gcgccacaat tgtcacgata accccgacgc
    51301 ctggatctgt catcggtgtt ggagatacca gatgttgaat acccgtcaaa aacaggaata
    51361 gtgccgaacc ggagataaac atactttgcg ccagcgccgc gagggactca gctttaccgt
    51421 gaccaaacga gtgattatcg tcggcaggtt gcagggaata tcgcaccacc agtaaattcg
    51481 tcaacgacgc gccgatatcc accagcgaat ccaccagcgc ggcgagaata ctcaccgacc
    51541 cggtatacca ccatgcaaaa attttaatca gcaatagcaa cgaagccatc gccgtcgcag
    51601 caatcgccgc ccgactgacc agccgtccat aagattgatt cataaatact cccgctatca
    51661 actgacgcta gtataacgga agcaaatcat ctgcaatgca ttaagcagca ggcaaattga
    51721 ggataaaaaa aaccccacat catgtggggg aagacaggga tggtgtctat ggcaaggaaa
    51781 acagggttta ctactgggaa cgtgagttgc tactactcaa tagcttcaac gatgaacttt
    51841 tttgccattg cgtcacgtca cgcaactgct ccattcgttg ttgatgtttc tcgtttaaaa
    51901 ccgcttgctg ctccggcgtt aacaggcgat acatttggtt gcggactttt gccatctcaa
    51961 cctgacgagc aatttgctca ttcgccattt tttctgcctg tgcgcgcaca gcgttttcat
    52021 caaaattttc tgcggtgaca aggcgatgca ttgtctccag ttcgctaaca ttaacaggag
    52081 gctgttcgtg ccgggcctgt tgcataagat ctcgcatctg ctgacgctga tgttcggtta
    52141 aacttatgcc gtcgaacata tggctctgcg tactgcgctg cgtaagttct tcacccggat
    52201 gccagttatc gcctgaaccg acttcagcag cgtggcttaa tgaactgact gccagcgttg
    52261 aggccatgac ggcagcggta actatgcgca tcatttgctc ccaaaatctt tctgtcgcga
    52321 ttcaacgata gagagtttac gattcaggct gcaaacatgc gtcagggggt gtaaaacaac
    52381 gtaaagtcat ggattagcga cgtctgatga cgtaatttct gcctcggagg tatttaaaca
    52441 atgaataaaa tcctgttagt tgatgatgac cgagagctga cttccctatt aaaggagctg
    52501 ctcgagatgg aaggcttcaa cgtgattgtt gcccacgatg gggaacaggc gcttgatctt
    52561 ctggacgaca gcattgattt acttttgctt gacgtaatga tgccgaagaa aaatggtatc
    52621 gacacattaa aagcacttcg ccagacacac cagacgcctg tcattatgtt gacggcgcgc
    52681 ggcagtgaac ttgatcgcgt tctcggcctt gagctgggcg cagatgacta tctcccgaaa
    52741 ccgtttaatg atcgtgagct ggtggcacgt attcgcgcaa tcctgcgccg ttcgcactgg
    52801 agcgagcaac agcaaaacaa cgacaacggt tcaccgacac tggaagttga tgccttagtg
    52861 ctgaatccag gccgtcagga agccagcttc gacgggcaaa cgctggagtt aaccggtact
    52921 gagtttaccc tgctctattt gctggcacag catctgggtc aggtggtttc ccgtgaacat
    52981 ttaagccagg aagtgttggg caaacgcctg acgcctttcg accgcgctat tgatatgcac
    53041 atttccaacc tgcgtcgtaa actgccggat cgtaaagatg gtcacccgtg gtttaaaacc
    53101 ttgcgtggtc gcggctatct gatggtttct gcttcatgat aggcagctta accgcgcgca
    53161 tcttcgccat cttctggctg acgctggtgc tggtgttgat gttggttttg atgttaccca
    53221 agctcgattc acgccagatg accgagcttc tggatagcga acagcgtcag gggctgatga
    53281 ttgagcagca tgtcgaagcg gagctggcga acgatccgcc caacgattta atgtggtggc
    53341 ggcgtctgtt ccgggcgatt gataagtggg caccgccagg acagcgtttg ttattggtga
    53401 ccaccgaagg ccgcgtgatc ggcgctgaac gcagcgaaat gcagatcatt cgtaacttta
    53461 ttggtcaggc cgataacgcc gatcatccgc agaagaaaaa gtatggccgc gtggaactgg
    53521 tcggtccgtt ctccgtgcgt gatggcgaag ataattacca actttatctg attcgtccgg
    53581 ccagtagttc tcaatccgat ttcattaact tattgtttga ccgcccgcta ttactgctga
    53641 ttgtcaccat gttggtcagt acgccgctgc tgttgtggtt ggcctggagt ctggcaaaac
    53701 cggcgcgtaa gctgaaaaac gctgccgatg aagttgccca gggaaactta cgccagcacc
    53761 cggaactgga agcggggcca caggaattcc ttgccgcagg tgccagtttt aaccagatgg
    53821 tcaccgcgct ggagcgcatg atgacctctc agcagcgtct gctttctgat atctctcacg
    53881 agctgcgcac cccgctgacg cgtctgcaac tgggtacggc gttactgcgc cgtcgtagcg
    53941 gtgaaagcaa ggaactcgag cgtattgaaa ccgaagcgca acgtctggac agcatgatca
    54001 acgatctgtt ggtgatgtca cgtaatcagc aaaaaaacgc gctggttagc gaaaccatca
    54061 aagccaacca gttgtggagt gaagtgctgg ataacgcggc gttcgaagcc gagcaaatgg
    54121 gcaagtcgtt gacagttaac ttcccgcctg ggccgtggcc gctgtacggc aatccgaacg
    54181 ccctggaaag tgcgctggaa aacattgttc gtaatgctct gcgttattcc catacgaaga
    54241 ttgaagtggg ctttgcggta gataaagacg gtatcaccat tacggtggac gacgatggtc
    54301 ctggcgttag cccggaagat cgcgaacaga ttttccgtcc gttctatcgg accgatgaag
    54361 cacgcgatcg tgaatctggc ggtacaggat tggggctggc gattgttgaa accgccattc
    54421 agcagcatcg tggctgggtg aaggcagaag acagcccgct gggcggttta cggctggtga
    54481 tttggttgcc gctgtataag cggagttaaa ctccgcattt gtaggcagga taaggcgttt
    54541 acgccgcatc cggcatttga gcaggatgcc tgatgcgacg ctgatagcgt cttatcaggc
    54601 ctacactccg ggggttattt tccccacaac cggcgggaaa aatcttcgat cttgccgctc
    54661 agtcggcgct tttgcatcgt ccgcgtccag cttttcgata acccggcggc ggagagtaaa
    54721 cggtgatact gatcgtcatc aaacggcata tgccatgcga tggcggcagc ctcctgcacg
    54781 gtcacatcgc tgacacggga aaccaactcc agcggcgcgt ccgcagacac tttccctggc
    54841 gctatcacgc tatacaacca ccccacctta cctgtgtttt gcatcaactg cgctatatcg
    54901 ctgatatcaa aatggtaatt gagtttatag caaggcgagc gcggctggct gacctgaatt
    54961 aatgcctctc cccagcggaa aatatcgccc atatgaacat tactttccgt gagaccgtcg
    55021 gttgagagat tttcgccaaa cgcaggcgca acaaacaact ccgcctgttc cggaaattcc
    55081 cgcacccagt agagatagtg ctcacgagga taatgacaca gcgctctgtc tggcccgccg
    55141 tgaacctttt tctccgcctg ctcgtcacct tccagcccca gctccgtcag catcaactca
    55201 ccatcaacct ggattttggc aattgcgctg ggtttgcctt cgggataagc ctgaatcttg
    55261 cctgtgtata catcaaccgg atatcgcatc tgttccctct ccacgagaaa tttccccatt
    55321 agaccatatt tattttatgt cagccgtgct gttttcgcgg taagtatcca ggcaggaact
    55381 caatagcaaa ccggacgaac gttagttcat ccggctgtgg g
//
LOCUS       DAZXUL010000009        54585 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZXUL010000009 DAZXUL010000000
VERSION     DAZXUL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54585
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.009"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="ACH3L1_000510"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="ACH3L1_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFT4075400.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="ACH3L1_000511"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="ACH3L1_000511"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFT4075401.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="ACH3L1_000512"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="ACH3L1_000512"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFT4075402.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACH3L1_000513"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACH3L1_000513"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFT4075403.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACH3L1_000514"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACH3L1_000514"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFT4075404.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="ACH3L1_000515"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="ACH3L1_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFT4075405.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="ACH3L1_000516"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="ACH3L1_000516"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFT4075406.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="ACH3L1_000517"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="ACH3L1_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="ACH3L1_000518"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="ACH3L1_000518"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFT4075407.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="ACH3L1_000519"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="ACH3L1_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFT4075408.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACH3L1_000520"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACH3L1_000520"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFT4075409.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="ACH3L1_000521"
     CDS             complement(13538..13933)
                     /locus_tag="ACH3L1_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFT4075410.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="ACH3L1_000522"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="ACH3L1_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFT4075411.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="ACH3L1_000523"
     CDS             complement(15220..15447)
                     /locus_tag="ACH3L1_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFT4075412.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="ACH3L1_000524"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="ACH3L1_000524"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFT4075413.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="ACH3L1_000525"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="ACH3L1_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFT4075414.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="ACH3L1_000526"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="ACH3L1_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFT4075415.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="ACH3L1_000527"
     CDS             complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="ACH3L1_000527"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFT4075416.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19688..19764
                     /locus_tag="ACH3L1_000528"
     tRNA            19688..19764
                     /locus_tag="ACH3L1_000528"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19722..19724,aa:Met,seq:cat)
     gene            19798..19874
                     /locus_tag="ACH3L1_000529"
     tRNA            19798..19874
                     /locus_tag="ACH3L1_000529"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19832..19834,aa:Met,seq:cat)
     gene            complement(19949..21202)
                     /gene="amiC"
                     /locus_tag="ACH3L1_000530"
     CDS             complement(19949..21202)
                     /gene="amiC"
                     /locus_tag="ACH3L1_000530"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFT4075417.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21434..22765
                     /gene="argA"
                     /locus_tag="ACH3L1_000531"
     CDS             21434..22765
                     /gene="argA"
                     /locus_tag="ACH3L1_000531"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFT4075418.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22827..24653)
                     /gene="recD"
                     /locus_tag="ACH3L1_000532"
     CDS             complement(22827..24653)
                     /gene="recD"
                     /locus_tag="ACH3L1_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFT4075419.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24653..28195)
                     /gene="recB"
                     /locus_tag="ACH3L1_000533"
     CDS             complement(24653..28195)
                     /gene="recB"
                     /locus_tag="ACH3L1_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFT4075420.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28188..31076)
                     /gene="ptrA"
                     /locus_tag="ACH3L1_000534"
     CDS             complement(28188..31076)
                     /gene="ptrA"
                     /locus_tag="ACH3L1_000534"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFT4075421.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31252..34620)
                     /gene="recC"
                     /locus_tag="ACH3L1_000535"
     CDS             complement(31252..34620)
                     /gene="recC"
                     /locus_tag="ACH3L1_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFT4075422.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34633..34983)
                     /locus_tag="ACH3L1_000536"
     CDS             complement(34633..34983)
                     /locus_tag="ACH3L1_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFT4075423.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34968..35375)
                     /locus_tag="ACH3L1_000537"
     CDS             complement(34968..35375)
                     /locus_tag="ACH3L1_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFT4075424.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35372..35935)
                     /locus_tag="ACH3L1_000538"
     CDS             complement(35372..35935)
                     /locus_tag="ACH3L1_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFT4075425.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35926..36396)
                     /locus_tag="ACH3L1_000539"
                     /pseudo
     CDS             complement(35926..36396)
                     /locus_tag="ACH3L1_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36580..37374)
                     /gene="thyA"
                     /locus_tag="ACH3L1_000540"
     CDS             complement(36580..37374)
                     /gene="thyA"
                     /locus_tag="ACH3L1_000540"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFT4075426.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37381..38256)
                     /gene="lgt"
                     /locus_tag="ACH3L1_000541"
     CDS             complement(37381..38256)
                     /gene="lgt"
                     /locus_tag="ACH3L1_000541"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFT4075427.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38407..40653)
                     /gene="ptsP"
                     /locus_tag="ACH3L1_000542"
     CDS             complement(38407..40653)
                     /gene="ptsP"
                     /locus_tag="ACH3L1_000542"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFT4075428.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40666..41196)
                     /gene="rppH"
                     /locus_tag="ACH3L1_000543"
     CDS             complement(40666..41196)
                     /gene="rppH"
                     /locus_tag="ACH3L1_000543"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFT4075429.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(41549..41695)
                     /gene="ygdT"
                     /locus_tag="ACH3L1_000544"
     CDS             complement(41549..41695)
                     /gene="ygdT"
                     /locus_tag="ACH3L1_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFT4075430.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     HGIG"
     gene            41881..42570
                     /gene="mutH"
                     /locus_tag="ACH3L1_000545"
     CDS             41881..42570
                     /gene="mutH"
                     /locus_tag="ACH3L1_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFT4075431.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42639..43352
                     /locus_tag="ACH3L1_000546"
     CDS             42639..43352
                     /locus_tag="ACH3L1_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFT4075432.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43490..43708
                     /gene="ygdR"
                     /locus_tag="ACH3L1_000547"
     CDS             43490..43708
                     /gene="ygdR"
                     /locus_tag="ACH3L1_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFT4075433.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43816..44856
                     /locus_tag="ACH3L1_000548"
     CDS             43816..44856
                     /locus_tag="ACH3L1_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFT4075434.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44889..46079)
                     /gene="lplT"
                     /locus_tag="ACH3L1_000549"
     CDS             complement(44889..46079)
                     /gene="lplT"
                     /locus_tag="ACH3L1_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFT4075435.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46072..48231)
                     /gene="aas"
                     /locus_tag="ACH3L1_000550"
     CDS             complement(46072..48231)
                     /gene="aas"
                     /locus_tag="ACH3L1_000550"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFT4075436.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFMPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48817..49848
                     /gene="galR"
                     /locus_tag="ACH3L1_000551"
     CDS             48817..49848
                     /gene="galR"
                     /locus_tag="ACH3L1_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFT4075437.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49855..51117)
                     /gene="lysA"
                     /locus_tag="ACH3L1_000552"
     CDS             complement(49855..51117)
                     /gene="lysA"
                     /locus_tag="ACH3L1_000552"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFT4075438.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51239..52174
                     /gene="lysR"
                     /locus_tag="ACH3L1_000553"
     CDS             51239..52174
                     /gene="lysR"
                     /locus_tag="ACH3L1_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFT4075439.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52161..52853)
                     /gene="ygeA"
                     /locus_tag="ACH3L1_000554"
     CDS             complement(52161..52853)
                     /gene="ygeA"
                     /locus_tag="ACH3L1_000554"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFT4075440.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52982..54400)
                     /gene="araE"
                     /locus_tag="ACH3L1_000555"
     CDS             complement(52982..54400)
                     /gene="araE"
                     /locus_tag="ACH3L1_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFT4075441.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        13350 a        14764 c        13458 g        13013 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 cgggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttctaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggct gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgccgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcaggggac ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcccctgtca tatccgtaaa
    18361 aacggcatac agaatatcac atcagccgct aaagacgtta cctgcgcccg gagccgtttt
    18421 cagcacccaa acccgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc
    18481 gatcccttga tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac
    18541 catcagacgc aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc
    18601 tgccagcaag gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc
    18661 aaccagcggc accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac
    18721 gaaagacggg ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca
    18781 atgacgaatc gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac
    18841 cttaccaatg ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg
    18901 actgccatca ccaaagtcga tatacccact accctgcaca tccataatga agttatccat
    18961 cagagagttg ctgtaagcga ggatgtattt atcgcccaat gccccggcgt agatctccgc
    19021 acgagacggc agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc
    19081 ctggcgggta tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc
    19141 atagttgtcg gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt
    19201 atcaccgcct gcgcgcagcc actcttgcac cgcgttataa acattacttt ggttgccata
    19261 cagacgcggt gacgaattac ggatatggtt aatttgctcg gcaaaatcac cggcgttaat
    19321 cggcgcgcca acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc
    19381 tttatattgc tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac
    19441 cgtgcccata agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa
    19501 aaataaggcg caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct
    19561 gacgcaaatt ttgcacaaaa aataggcttt agtggtttgt ttttgttcaa aatcatgcta
    19621 aatccgtgat cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat
    19681 ccacggacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg
    19741 gttcaaatcc ggcccccgca accaatcaaa tttgatgaag taaaagcagt acggtgacgc
    19801 ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc
    19861 ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct gatctcccat
    19921 aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtt gccccatcgg
    19981 caaaatacgc tttaatcccc gcaagaatag actccgcaac ttcctgctgg aaagtcgccg
    20041 ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc agaatggagg
    20101 gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt ttatgcagct
    20161 tgttgatttt tccgagctta ttcagtaccg ctttaccaaa cttaaggctg tcagcaatag
    20221 tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg cttttgctca
    20281 cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc gcagtactgg
    20341 ttgcgccttt ggttgagagc gcaaacaccg aggaaccgct cggctgacga ctggtaaagg
    20401 cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt gctacgcgca
    20461 cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata ttgccctctt
    20521 cctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt tcgcgcgttt
    20581 tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc ataatgacaa
    20641 tcggacgatc gcgccctgct ttacccggtt gtggaccact ttgcgctggc ggcacctgct
    20701 tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc tgcatatcct
    20761 gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg accggcgcaa
    20821 gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt acggtttgcg
    20881 ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga atttgtgccg
    20941 ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc acgcgttcag
    21001 ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc gtcacgcggg
    21061 tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca gccaggctga
    21121 cctgacttac gctcaatagc cacatagcgc ccgcgccttg cagtaaacga cgacggctga
    21181 ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc tgtgtaacaa
    21241 gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc atctaataaa
    21301 cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc tcgcataaaa
    21361 tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcaa ataatcatgc
    21421 aaaggggtgt gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat tccgccattc
    21481 ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg gcggtgaagc
    21541 cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc acagcctcgg
    21601 catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc tggctgcgca
    21661 tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa cactggaact
    21721 ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt caatgagtct
    21781 caataacacg ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt ttattattgc
    21841 ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta tccggcggat
    21901 tgatgaagac gcgctccatc gtcaactgga aagcggtgca atagtgctaa tggggccggt
    21961 cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg ccactcaact
    22021 agccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccagg gcgttactaa
    22081 tgacgacggt gatattgtct ccgaactttt ccctaacgaa gcgcaagcac gggtagaagc
    22141 ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg gcgcagtgaa
    22201 agcctgccgc agcggcgtgc gccgctgtca tttaatcagt tatcaggaag atggcgcgct
    22261 gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg aaagcgccga
    22321 gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga ttcgcccgct
    22381 ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg aaatcgacaa
    22441 attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct atccgttccc
    22501 ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc gcagttcatc
    22561 acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg gcttaagcaa
    22621 attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat ttaccccggt
    22681 ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc gtaaatccaa
    22741 agtgttgatg gcggatttag ggtaagcgat gaaaatcacc ggatgcggca ggcgtaacac
    22801 tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac taaacaacgc cgccagacca
    22861 ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc ggcatacagc
    22921 gacagacgac gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt tactaccggc
    22981 gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg cgatttatgt
    23041 accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac agacttaata
    23101 ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag cgcaataccg
    23161 atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcatcac cggtcggcct
    23221 tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat aaactgttca
    23281 attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca caaaagctgg
    23341 tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag cagatccaga
    23401 taacgtccgt aacccgcaag agcttcctca agcatcgcaa tataatcttc gccgctctgt
    23461 aaaagtcgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac tgccgtttta
    23521 tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcgctgcc gaaacgatag
    23581 cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc ggttcctgcc
    23641 ggaacgtgag tccccgtcag gcggcttagc tgcctggcac gctcggcggt aaagcccgcg
    23701 ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc cagttgatca
    23761 cgatcgccga gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag tctcgacatc
    23821 ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg cagcgggtta
    23881 ccggcatgat gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg gtgcaaagtg
    23941 ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg tcgcaaagcc
    24001 ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc cagacggata
    24061 cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gcaacttcgc cacggtggtc
    24121 gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag cgccactgcc
    24181 gctgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc cagggtttgc
    24241 gccagtagag cttcatcaac ctcaatggta tgattcacct cattgaagaa gcgcgctacc
    24301 gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag gatcatcggc
    24361 gtgggttcat ctcccctgct gaccgcttga gaagccagca agcattcttc ccaattttgt
    24421 agctcaccga tttcactgac acaggtcgcc aacagcggat tcgacgcctc gttattttcc
    24481 agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa cagtgccgcc
    24541 gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg cacatccagc
    24601 gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt cattacgcct
    24661 cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg gcgttgggtc
    24721 gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg aacagataga
    24781 taacgccgcc aaagtgaaga tcatagtcgt aatcagcaat gcgatggcgc agataacgat
    24841 gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc atcgctgctg
    24901 ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat ttatagtcga
    24961 gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt aacatgccgc
    25021 gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac tggcgaatta
    25081 gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac tccatctcca
    25141 cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg agaggtgcct
    25201 ggaggacagc cgtgatccac tcggtcaaca ccggttccca ctgcgattca aagccgccga
    25261 gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg aggtcttcaa
    25321 acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc ggtgttaacg
    25381 tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt ggcatcagat
    25441 cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca cgccagttat
    25501 cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc agttaaagca tcattaacct
    25561 gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca tcgcataacg
    25621 cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc agcaaacgcc
    25681 cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg cgcaccagcg
    25741 gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa agcaaacgca
    25801 gatcttccgc cagacgttcg acctccgcga ggtcgacgct ttctggcgca gcattaagat
    25861 ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg acgcggaaat
    25921 tggtaataaa tggcaaccag accagcggat attccagccc tttcgattta tggatcgtga
    25981 caatctgcac cagatgttta tcactttcga ggcgcatttg ttggctggag gcattactgt
    26041 ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt tccagctgcg
    26101 ttccggcttc ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc cgctcaccgc
    26161 ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga catcagcgcc cgcagcatcg
    26221 gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg actaccgcat
    26281 cccacgcatg ttcgtcatta ttcagcgttt caatatccag cgcattcagc cccatcattg
    26341 acgttgccag cgcactgcgc agggtgttct cacgttcggg cgtcatcacc gcctgcaaca
    26401 accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc gaaaggtaaa
    26461 cggaagggat ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc tggcggctgc
    26521 gcaccagcac gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg ttcatcagca
    26581 acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca catacctgcg
    26641 ccatggtact ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc cacattttca
    26701 tcgcaggctg cgtttcacct ttaaatacaa aacgtaacgc ctgatttttc ccggctgatt
    26761 tcactggaat aaacggtatt tcgcgaaaca tgaacgtgtc atcagtctgg ctgaaaagct
    26821 tgttcacact gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa gtgtagtggg
    26881 catgaacttc actacgcgcc ttcatataag tgaagatatc cgcaccccgg aatgcatata
    26941 tggcctgctt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg tgccagatac
    27001 ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc attgccaccg
    27061 ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct ttcgctacgc agcgcggaat
    27121 cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt tcacgcgcta
    27181 ctgtttcgcg gatctcagcc aatgcgcggg tgatcaacag atcgcggatc gacaatggtt
    27241 ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc cccccggcct
    27301 tcgtgcgatc ttctaagaaa cgctgggaga atttttccag cgactccggc aactgataac
    27361 tgtttgtctc ttcttctgcc caggcgctga tcttttcgat ccatttagcc tgattgctac
    27421 ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt tcacccactg
    27481 cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg tggcgggaag
    27541 ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg cggttcgcct tgcagataac
    27601 gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg acctgggcta
    27661 tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc tggtagcgta
    27721 gcagagactc atcttcaatc agctgctgct caaacagcat gccggattca aaggcattca
    27781 ggttgagcat gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca tccatctgcc
    27841 gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct tccagcaggc
    27901 gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac tcgtggatat
    27961 tgctacggat acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg accaccagca
    28021 gttcttcaac gatcagcggg cggggaaagg cggcggaacc gcctagtcca agtaacaggc
    28081 gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct tcaatcaggc
    28141 gctcgccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca ctcattcttt
    28201 tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac tttccagcct
    28261 tcaggatgca catattcggc tttcccgttc tggctgccgg aaatctgcga cagaatagcc
    28321 atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg cggcgtcagc
    28381 agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc gcgatcgaaa
    28441 tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag catctgggta
    28501 attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa ttttgcctct
    28561 gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt atcattgctt
    28621 tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc aaacacggca
    28681 tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac gatctgcccc
    28741 aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac aaataccgct
    28801 gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg ttttttatcg
    28861 accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg tttttgcaca
    28921 tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat aaactctggt
    28981 cgagcccctg attttaaggc gtcgcgatag gccagcacct ctttcaacgt aatggagggc
    29041 aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat ctgcgcgggc
    29101 ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg gttataccag
    29161 gacttcgcct gctcaagctg ctcttccgta gcggtatagc taaagtaccc ctcgagcaat
    29221 gcctggaaca gctgcggcag acgctgggtg taaccattag cattaatcat aaggccgttg
    29281 ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa ctgatcaagc
    29341 gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc gctgtccatg
    29401 gctttcggat tacgcaaaat caggctgaca tcagctttgg gctcgctggc aaaataacgg
    29461 cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg atggtcgtat
    29521 ttcttctctg acttaatcag cgagaaatca tcaggaatat aagggttaag ctctggcaaa
    29581 gagagcgcaa tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc gctgatttta
    29641 tcgacctgat acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc tttcgggctg
    29701 atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt tactgcttta
    29761 gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaacagg aacgcgaatc
    29821 atggtatctg ccagccattc gacgtaatcc atatcacggg tgatcgacgg ataacggaag
    29881 tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcaatgcc tttttcacgt
    29941 aacagattga gatagctaaa aattgccgcc acaacctgat cgcgattagc caggccttta
    30001 tcagttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat aggatcggag
    30061 ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcagaaag tgtacctggg
    30121 ctgcgattgc caatcagata ggtaatcaat tcatcggttt tactacggaa cttcgctgag
    30181 ttgttatcga tgcgaaactc aacgcgcaac actttacgcg gcagcgcagg gacgtaatga
    30241 atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg ttttttgctc
    30301 tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc cggcagcggt
    30361 ttattactgt aaataaccgc cttcatcaaa ttggcggaat agtacttctc gtggaaatct
    30421 ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta aagtttcgag gttaccacca
    30481 gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg tgccatgcgc
    30541 atcccgtcac gcgtacgcgc catggttaat tcagcgttca ctgcattacg ctcacgttcg
    30601 gcgtatttct tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc taccgcaccg
    30661 ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc agtgctggca
    30721 ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg cgggtacttt
    30781 ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta cgcctcggga
    30841 tcttccagcg acccaacggg cactaccagc gccgagagcg atttaactgc ctgcggatca
    30901 gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg gcggttatct
    30961 ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc ctgacttaat
    31021 ggtgcccaga gggcaacaaa caacaataat gctttgaacc aggtgctgcg gggcattcac
    31081 ggacctcata atcaacttta ttttctggac agattcaaca ggttaacgca aatcatctgc
    31141 cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc agtccgtgac
    31201 tggtgcgcat cataaagtaa gcagatagat tgcgcaattt ttatacaaca ctcatgactg
    31261 attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg cctccattgt
    31321 ctctggtgtt aattgccgcc agagccgttg ataccagata tcatcacctt cgccacgcac
    31381 catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca acgtggaatc
    31441 gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg cgccgccact
    31501 ttcaggtaac accagcaatg gcgcggacat tccttcacga tacccctcaa tcagttgtga
    31561 gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcac cgtctttgcg
    31621 tagaaaaagg cgactttcgc cattaccacc gctggcacag tagacaaggt gttccagcca
    31681 aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca ggccatccgg
    31741 ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat caatttccat
    31801 actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca tctcctggca
    31861 ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg ccgcccggaa
    31921 gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata actgctgatt
    31981 gatttgataa cggctaagtc cttccagaat aaatggctcg gtgtctggga tttcgctgtc
    32041 ttcagtatgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg gatgtgccca
    32101 gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca gcggctgaac
    32161 aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac gagcatagct
    32221 ttgtcgttcg cctggctggt agttttgcgg atcaaacggc atccgggtat ggaggcaagt
    32281 aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat cgcccggtag
    32341 ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg ggaaacgttc
    32401 actgttatcc tgaatggaac gcccgatata gctgatatag agtttttgct gcgcggaaat
    32461 taacgcttcc aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc cacgcttcgg
    32521 tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa cgccgtcgtt
    32581 cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca gagtacaaat
    32641 gttaaccggt ccggcgagaa aacgctggct gatacgttct tgatccagac gctgtgccag
    32701 ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta aaccttcggc
    32761 gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt ccgcatccgg
    32821 caggaagaag gcgttgagca tatcgcgaca aaccggcaac cactcttcta gcggacgctc
    32881 ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agtgaagcca gatgccccac
    32941 cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc actcgccctg
    33001 cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc aggtgtgttg
    33061 tccggtggcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc aacgaatgcc
    33121 ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt caaaccgcgc
    33181 cgccagcacc ggcacatcca gcagcgccag cacatcctct gacacaaagc gactgtcagg
    33241 cagtgataac aggctgataa acgcttccag taccggatgt gactgccgcg cacgacggtc
    33301 ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacactg cctgaataaa
    33361 cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag taagtgtcgg
    33421 ggcttcctcc agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac gctgcgggct
    33481 atggcaaacg tggaatgtga tactgctatc cagtggatca agcgggcgtt tgttatcgct
    33541 acgggaaaac tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt ccagaatgtc
    33601 agactgaatg ttatgcagca ggttatctgg cgtcacatcg acaaaagcgt ccagctcctg
    33661 gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc cccatgaggc
    33721 cagcagcgag ttgccgacat cctgttcacc atcgctgtta aagagctgtc cggcattttc
    33781 gctatcgcga aatagcggca attcgcgatc ttcaaaactg tgtcgacgct gacgagtcag
    33841 cagtttcgcc agataagcag gatctttaat atcgccccag taataacggc aggggttggt
    33901 aaacaggaga tggatttcaa tatgtttacc cagcgcctgg agcgcctgga gataaacagg
    33961 cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg ggcaggtcgt
    34021 cgcggactcc agcgtttcga taaagcgctg atagagattc gcgcggtgcc agcgcggttg
    34081 tccgagttca tgggtatatt ccaccagcgc cttccacaac ggggcttgcc aggcctgtgc
    34141 ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt ccggacgata
    34201 gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca gttttcgctt
    34261 gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct ccagcaattg
    34321 cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc tctctttggg
    34381 gatttccggt aacacccgga cgaacatatc ccagataaag ctcgctggca gcggaaaatc
    34441 aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact gcgccatacc
    34501 ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt cgcgttcgac
    34561 aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga cccttaacat
    34621 agcggctcct gactactgac aattcgggca atgcaggcgg gtcatctcgc cttctctgcc
    34681 cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg tctgcattcg
    34741 gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct gccaaccatg
    34801 ccgccagagc ttttggtact ggtttctgct ggcgagactg ttcattgatg ttcgctggat
    34861 acccgataac gcagtgacaa tcatcaccat cagcaccatc agcaccatca gcaccatcag
    34921 caccatcgca accattacct ccggcaggct aaagccttgt tgattcttca gggaagctga
    34981 cataatgccc cctctttcag cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatt
    35041 tttccatcaa tgacttcgcc tgttcgccac aacgaaacgc cttcataacc ggcaatcaat
    35101 aaggcttcat tatctgccag taaacgcaaa caaacctggg catcggttcc agcgtactgc
    35161 gagcactgaa ctgctggctg cgtctgccag gagtgcattt ttccccacgc cagcgccgac
    35221 tgaacgatgg cctggcggcg caatgactga ctttccatgc tcacgcgaga ggcaaaactg
    35281 cgatcctgct gactcattcc ttgcaatagc aagctaccca aaaccagcag catcaggacc
    35341 agagccagtg acgaaacacc tttttcgcgg ttcacaggtt gaatcctgtc acgctatagc
    35401 tggcatccac cacggtttgc ggttcagact tactggcagc acgcatatta accgtcaaca
    35461 ccggcgagaa gccgctgaca tcctgacgta cgacctgaaa agtgtcgata atgatggcat
    35521 ccggattagt gactttatcc cagcccttac cttcacagga tgtcgcaccg cgtagcgttt
    35581 ccagcacatg ctccttcaga cgaaatccaa tctggtcgga ctcttttacc ggttcgcgat
    35641 cccagatacc gttactgttc gcatcccact gcacaatgat acagtcaccc tgtccgacaa
    35701 tttccagccc ttcgccggta cagctgccat gacaataacc cgccctctgg agatgcttcg
    35761 cgacggtaaa tacccgcagc cagatttcat cttccagtgc cagcttacga gtgctcgtta
    35821 aactttcacg ctgtaacgca ggcagaaagc gtgcagcccc cagcaacaat acgctactga
    35881 tcgccatagc aatcaacact tccagcagag aaaaaccttg ctcttttaca ggcatccttc
    35941 tgtttctcct tgctgataaa gccggagtct tccccacggc gaaaccacca gccaccactc
    36001 gcccgttgag tttttgaagc gaatatgcac ggcccatgcg gtattgcgca ggccaaagaa
    36061 agcaagcgaa ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca caaagaccaa
    36121 tggtgaactg ccatgacagg tattggcccc agcagcggaa ctcacaaggc accataacgt
    36181 cccctccctg ataacgctga tactgtggtc gcggttatgc cagttggcat cttcacgtaa
    36241 atagagcaaa tagtcccgcg cctagctggc ggtttgccaa agccgttgcg actgctgcca
    36301 gtattgccag ccatagagtc cacttgcgct tagcatgacc aaaatcagca tcgcgaccag
    36361 cgtttcaatc agcgtataac cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa
    36421 aaaaagacga gggccagttt ctatttcttc ggcgcatctt ccggactatt tacgccgttg
    36481 caggacgttg caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg taaaaaaacc
    36541 gacgcacact ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt
    36601 aacgcccgga tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt agtcgaagat
    36661 ggattcgggt ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg
    36721 cagatgagtt tgatccatat ggttgctgta caggtgcgta tcgccaccgg tccagacaaa
    36781 atcacccact tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc
    36841 aatgttgaac ggcaggccga ggaagacgtc acaggagcgc tgataaagct ggcaagagag
    36901 tttgccgtct gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc
    36961 cagttcgcct acgttccacg ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag
    37021 ctggttcagt accgtagtaa tctggtcaat atgacgacca tctggcgttg gccaggcgcg
    37081 ccactgttta ccatacactg gcccgaggtc gccattttca tcggcccatt cgtcccagat
    37141 ggtgacattg ttttcgtgta gataagcaat gttagtatcg ccctgcagga accacagcag
    37201 ttcgtggatg atggaacgca ggtggcaacg tttggttgtc accagcggga atccatcctg
    37261 caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt
    37321 tttctgtgtg ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc
    37381 tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc
    37441 gacaatcatc gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccggt
    37501 aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtagccaat
    37561 caggaacaag cctgagacag ctcccattgg gcgcggttta cgaatataca ggttgaggat
    37621 aataaacagc accacacctt ccagcagcag ttcgtaaagc tgtgatgggt ggcgcggcag
    37681 cacaccgtaa gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc
    37741 ttctgtacgg gagcctggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca
    37801 caattcaccg ttaataaagt tgcccagacg cccggcacca agaccaaacg gaatgagtgg
    37861 tgcgataaaa tcagagacct ggaagaagga acgtttagta cggcgggcga agataatcat
    37921 caccacgata acgccaatca ggccgccgtg gaaagacatg ccgccgtccc agacacggaa
    37981 cagatacagc ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg
    38041 tcccccgagg aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt
    38101 ccagccactg cccggacgat tcgcccgacg tgttgccagc cacattgcaa aaatgaaccc
    38161 caccagatac atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg
    38221 atcaaactcc ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt
    38281 tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc
    38341 gaataatagc acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga
    38401 tccgcgctat aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc
    38461 aacctgatga cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc
    38521 tgcttcggca aaatcaatgc gccgcagcag gtattttacc cgcgctacag aacgtccgtt
    38581 catagacaga tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc
    38641 catttcaccg cacaaacgaa gatcgatacc atgtatttcc gcttcccggg cgatcatcgc
    38701 cagagctcgt aacattgcag gatgaagact gtcgtaaatg ttcgccaccc gggtattgtt
    38761 gcgatcaacg gccagaatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac
    38821 cctctttgcc agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat
    38881 acgtggtttg ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat
    38941 caggcggcgt gcttcgtcaa cttcatcgag gctggtgacc atcggcaaca gaatattcag
    39001 gttgcccgta gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg
    39061 ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctgatcgg
    39121 catgtaaggc agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc
    39181 attgaacatt tgcagcatcc cctgatactg cgccacctgt tcttcttccg acgggaaacc
    39241 actttgcagc atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacagctgcc
    39301 cagtttttct tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc
    39361 gctttttaac tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc
    39421 ttcactaatt agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc
    39481 accgcgatag ccatcaacga tcagcgtcct acgatgcagc accgaaggct gaatatccgc
    39541 gcccatcacg gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctgc
    39601 gccatctcgc acgacaacac cgactaagcg atcctggggc agctcagcaa gagttgtcgc
    39661 tgacagttca tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc
    39721 gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt aaatcaccag cccgctcttt
    39781 gagatagtta tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac
    39841 cgcccactct gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt
    39901 gtccgaaagc aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgctccggc
    39961 ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag
    40021 tcgttcgcgt tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg
    40081 taacgtggca tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg
    40141 gattcgcgtc tggcgatatt gcccaaacag ggcagtcaac tgcgactgag aaagaatagc
    40201 tgccatctgg gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa
    40261 ctctcgttgc tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc
    40321 taaaaacgcg cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt
    40381 ttgcgcatct gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc
    40441 atcaaacgcg agcgttacag tgcgaccgcg tggttttttc agcccccggg tcgccatcag
    40501 gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc
    40561 aagacagatg tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac
    40621 cttttcgact atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg
    40681 tcgataagca gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact
    40741 cgcgaactct ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac
    40801 cggataccag taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat
    40861 attgatttct gcatcgccgc ttaccagctg caagagaaac catttttgtt tttggccgat
    40921 acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt
    40981 acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc
    41041 acggtacatc gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca
    41101 agagtgctga ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat
    41161 acctacgttt gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt
    41221 atatacgaat gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta
    41281 cacactaata acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg
    41341 tggatagccg tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac
    41401 tacgggaaaa cccggagcat tctgaataag cccgatatag acattttaaa atattcatta
    41461 ttagtcaatg cattacatgt aatttgagta atgaaaaaga gtaattcgtg acccaggtca
    41521 cacctctcat ttacgggttg gcgaaagatc aaccaatgcc gtgtttatcc acagaatgtg
    41581 ccactaagtt aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa
    41641 atgaaaacag tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt
    41701 aagatcctgt ttattttcag tgaccagatt tggaaaacct attgcagtgt tgcgcaactc
    41761 gattaccggc agcctaaaaa gcaatataaa tcagagaatt gaacaacaca tgtggaaaaa
    41821 gttacactgc gaatattcgg cacataattg ctgtttgttt tttaaccaag gtatcatgac
    41881 atgtcccaac ctcgcccact gctctctcct cccgaaactg aagaacagtt gttagcgcaa
    41941 gcacagcaac tttctggtta tacattggga gaactggcgg cacttgacgg gctggttacg
    42001 ccagagaatt taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt
    42061 gccagcgcag ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga acttaaaact
    42121 atccctgtgg atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgcccggtta
    42181 acgggtaata gcggggtgac ctgggaaacc agccatgtgc gccacaagct caaacgcgta
    42241 ctgtggatac cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg cgtaggatca
    42301 ccgttgctgt ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga ctgggaagaa
    42361 ttaatggata tgattgttct cggtcaggtt gagcggatca ccgctcgtca cggggaatat
    42421 ttacagataa gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat tggtgcccgg
    42481 ggcgaacgga ttctgacgct gccacgcggc ttttatttga agaagaattt caccagtgca
    42541 ctactggccc gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca
    42601 tataattacc gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg
    42661 atcctaacgc ttggcttgcg ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg
    42721 acaatattat tttcctttct ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg
    42781 cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatcaatct
    42841 cttgggttac gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa atttctgccc
    42901 gtgatttgat tctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc
    42961 acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg
    43021 ctatcgtgca gattatgctg ctggatatta tcttcagcct cgactcggtg attaccgctg
    43081 tgggtctgtc agatcacctg tttattatga tggcagccgt ggtgattgcc gtaggcgtga
    43141 tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg gtaaaaatgc
    43201 tggcgctctc tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc
    43261 acgtaccgaa aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca
    43321 acttgattcg caacaaaaag aatccgctct gatactccgt acgctctcct gcgggagggc
    43381 gtactcactc cttttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc
    43441 ggtttataaa cagactatta tcataggtag gccaaatgag gataaccgga tgaaaaaatg
    43501 ggcagtaata atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta
    43561 cgtcatggcg accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga
    43621 tgataccggg ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga
    43681 tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct ggccttaaga
    43741 tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat
    43801 aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg
    43861 ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa
    43921 ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta
    43981 cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg gctggcgaaa
    44041 catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat
    44101 gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat
    44161 gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca ctggccgcag
    44221 cgcccgacca actgcttcgg caaactcggt tatagctgga cggattctgc gcctgcggtt
    44281 tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa aattcgttat
    44341 atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa
    44401 cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa ccgcagtttt
    44461 gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc
    44521 ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt
    44581 aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg
    44641 gcgtatgttg atatcgccag acgtcatggc cttgatcctg ctcagatggc gctcgcgttt
    44701 gtacgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggatcagctg
    44761 aaaactaaca tcgaaagttt gcatctggag ttaagcgaag acgtattagc tgaaattgaa
    44821 gcggtacatc aggtttatac ttatcctgca ccataaagat taacgccggt taaaaccggc
    44881 gttaaatatt aatgacggcg ctgccagatc cacagcgccg ttattgccag cgcaaatagc
    44941 gcaccgaagc caatgccaat gggcacgacc gggatgccta ccattaccgc cagcgagtaa
    45001 atgcccagca tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc aatcgcattc
    45061 cccgccccga cgcttttttt accccgctcc tgtagcaacg cattgagcgg aacgacaaaa
    45121 aagcccccca gcacgccaat cagcatcaac aaggcatagg ctggcagcag ctcgtgttgc
    45181 agggagaaaa tcggtaccac cacgccaatc aaaatcccgg ctggcataca gcgtgacacg
    45241 gtttccagcg taaccagctt cgctgccgca cctgcgccaa ccacgatacc aatcgctacc
    45301 atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac cggtacccac
    45361 agcaccaaca ggaaacgcag cgtgacaccc gctccccaga ataaactggt gcccaccagc
    45421 gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg cattcaggaa actgcgggtc
    45481 atgttgatga gattccagga ctgccccgga cgcgccgcca gtttgggaat gtagatattg
    45541 gcaacgaccg caccaccgta ggccagtgcg catgcggcca gagcgaccag gacatgccag
    45601 tcagccagca caccaccggc tacggaaccg agtaaaatcg ccgctatggc agaagcttcc
    45661 attaaaccgt tagcttttac taacttacta cccgtggtta attcgccgag aataccgtat
    45721 ttcgccggtg aataggctgc agcaccaaca cccaccagcg tatagccgag aaacggattg
    45781 ataccaaagc agatactggc tgcgcccagc agcttcaggc cgttggcaaa catcatcacc
    45841 cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgccaaaag aatgtaagca
    45901 cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg cgctttcagt
    45961 aacgccagag tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa ctgcgccacg
    46021 ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga ctcactcatc
    46081 gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt tgccgctgcc
    46141 aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct ccggcacgcc
    46201 gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg tggtgaaaag
    46261 caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga ctttatctgg
    46321 cgaaacacca agtgccagtt gttccaccat ttccagcgac accatttcgc ctgcaatttt
    46381 ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa aacgcacaat
    46441 atcgccagtg tcataccagc cgcgctccat ttcgccgcga acattctcgg cggtgggcac
    46501 ttccagtaca cctggcttct ccacccgcag atagccgttc attatgttcg gccctttcag
    46561 ttgcaggcgt ccgccctctt cgataccagg gaccgacaac aggcgtgcat ccattcctgg
    46621 tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag aaacgacagg
    46681 cgcgcattcg gtcacaccgt agccttcaag gatgcgcagg ccaaatttat cctgccaaag
    46741 ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta gacgatagaa
    46801 gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac cgaacaacac
    46861 ggtgcaactg cggtcataca ccaagtccgg cacaatgcgg taatgcagcg ggcttggata
    46921 aaggaacact tctgcacctg taagcagtgg cataaacagg cctaccgtca gcccaaagga
    46981 gtgaaacagc ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg caatcgtttt
    47041 aatctgctcg acgttcgcca gaatgctttt atggctatgg acgacgcctt ttggatggcc
    47101 ttcagaaccg gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt ttacctgcgc
    47161 caggcgcggc atcagcaaat gagcgaagat ccatactttg tcggcagtgg tgacatctgc
    47221 ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca gatgccagag
    47281 tttgccttta tcgagaaact ggcgggaagt gaagatggtt ttgatttcag ccgccgtaat
    47341 agcactggtc agccctttta ccccggcagt gtagttcatc attgcgggaa tgcggcgacg
    47401 cgcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca ttaagccgat
    47461 gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg ttttcgtaag
    47521 caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct tcccggctcc
    47581 gaagcggtac attgcactca gtaaagattc gtacagagtt tcacgcgggc gcaccgccat
    47641 acgcgcttcc atcattattt gatgcagcat ttcgccagcg attttgcgac ggtcacgggc
    47701 acgcggcgca tccggcatcg ccacctgcgt tggaggcaaa atatgcagag taatttgcgg
    47761 gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt ccgccccttc
    47821 aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg cgccatcgta
    47881 gattttcatc agcgagcctg tagtggtgat gcgtccttca gggaaaatca ccactggtcg
    47941 gccctgttcc accagacgta ccagatgttt aatagccata ggttgcgtcg ggtcgagagg
    48001 aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta ttgaggtgta
    48061 aacggcaaac actggacgca caggtaaaaa cagtccaagc aaaatgccat caataaaaga
    48121 gacgtgatta ggcgtaatta gtacgcgctc gcccttcagt gcctgggtgt cacccgtaac
    48181 gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca ttccaactcc
    48241 ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa gaacgcgagc
    48301 gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg caagagacag
    48361 ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatct tgattgtggt
    48421 ctgcacgacg actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa tgcaacgagg
    48481 tgtgtaaatt gtcggttact gttacagatt gatgaccggc aaaaaaaaac ctgcgcatct
    48541 gcgcaggctg gtgtaattca tgtgctcaac acgaagttga cttcacctat caatacctct
    48601 gggatcacca ctttagcaac ctgaagccaa acgccaccag cggtcaatcg caacagcgtt
    48661 tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt cttacttatt tgcgtttggc
    48721 tcacattccc acgatgaaaa cacgccaccc cttgaaccaa cgggcgtttt ccgtaacact
    48781 gaaacaatgt aagcgtttac ccactaaggt attttcatgg cgaccataaa ggatgtagcc
    48841 cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc acccaaagcc
    48901 agcgaagctt cccggctggc tgtgcatagt gcaatggagt ctcttagcta tcacccgaac
    48961 gccaacgccc gtgcgctggc gcagcagacc actgaaacgg tcggtctgat cgttggtgat
    49021 gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc ttatcacacc
    49081 ggtaatttct tattgattgg caacggttac cacaacgaac aaaaagagcg tcaggctatt
    49141 gagcaactga tccgccatcg ctgtgctgcg ttggtcgtcc atgccaaaat gatcccagat
    49201 gccgatttag cctcattaat gaaacaaatg cccggtatgg tgctgatcaa ccgtatcctg
    49261 cctggctttg aaaaccgttg tattgctctt gacgatcgtt acggtgcctg gctggcaacg
    49321 cgtcatttaa ttcagcaagg tcatacccgc attggttatc tgtgctctaa ccactctatt
    49381 tctgacgccg aagatcgtct gcaagggtat tacgatgccc ttgctgaaag tggtattccg
    49441 gccaatgatc ggctggtgac atttggcgaa ccagacgaaa gcggcggcga acaggcaatg
    49501 accgagcttt tgggacgagg aagaaatttc actgcggtag cctgttataa cgattcaatg
    49561 gcggcgggcg cgatgggcgt tctcaatgat aatggtattg atgtaccggg tgagatttcg
    49621 ttaattggct ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct gaccaccgtg
    49681 cgttacccaa tcgtaacgat ggcgacccag gctgccgaac tggctttggc actggcggat
    49741 aatcgccctc tcccggaaat tactaatgta tttagtccga cgctggtacg tcgtcattca
    49801 gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca gcgactaacc gcagttaaag
    49861 caattccagc gccagtaatt cttcgatggt ctggcgacgg cgaatcaacc gcgcctgacc
    49921 attatcaaac agaacttctg gtaacagcgg acggctattg tagttggatg acattgatgc
    49981 gccatatgcc cctgtatcat gcagtaccag ataatcacct gccttcactt ccggcaaggc
    50041 gcgggtttca acatttcccc cttcctgctg ggtaaagaca tcgcccgatt cacataacgg
    50101 cccggcgacg acggtttcca ccgttggcgc gtgttccaga gaacgaccat cagctgccag
    50161 ggcactgata tggtggtaac taccgtacat tgccgggcgc atcagatcgt tgaacccggc
    50221 atcaaccagc acaaagtggc ggctacccat ttgtttgacg ctccgcacct gagtaattaa
    50281 tacgccagac tgcgctacca ggaagcgacc cggttcaatt tccagtttca cagggtggcc
    50341 caaatggcga gcgatttgct cacgcgcggc attccacaga ccataataat gttcggtatc
    50401 aaccgcctct tcaccctgtt gataaggaat agaaagccca ccgcccgcag aaatagcctg
    50461 taaatcctga ccgaattcga taacctgacg caccatagca ccacacacct gctccagatg
    50521 ggcataatca acgccagaac caatgtgcat gtgaatgccg actagctgca gatgatgacg
    50581 ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct tgctgttttc
    50641 gccaccggta ttggtttttt ggctatgtcc gtgaccaaac cccggattaa cgcgcagcca
    50701 tacccgatgc cctggcgaaa cctggcccag ttggtcgagc gtatcaacag aacccgcatt
    50761 caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa catctgccgt
    50821 aaaaacaata tcatcggggt gcgtttgcgg attgtaaccc gccgccaacg cacgctctat
    50881 ttcgcctaac gagacggaat ccactttcac gccctgctca cgcattaagc gcaaaatatg
    50941 aatattggaa caggctttct gtgcaaagcg caccacttca aactgtttca gcgctgcaat
    51001 ctgccgacga ataatttgcg catcgtagac ccacaccggg cagccaaatt cagcgggcaa
    51061 acgcagcaga ttttcggcgg tgagatcggt atcggtgctg aacagtgaat gtggcataac
    51121 aaactccaga tgagtgcttt tttatgatta cgccacatca taaaaagaat aaaaaatatc
    51181 gatttatgtc gagtctatgc aaaaatgata tggattaccg gattgcgaga gagcgctaat
    51241 ggccgccgtt aacttacgtc atattgaaat ttttcatgcg gtaatgaccg ccggaagcct
    51301 gactgaggca gcacacctgc tacacacctc acagccaacc gtcagccgcg aactggcgcg
    51361 ctttgagaag gtgatcgggc tgaaattgtt tgagcgcgta cgtggacgat tacatcctac
    51421 cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac tggatcgcat
    51481 tgtcagcgcc gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta ttgcctgcct
    51541 gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg cacgttatcc
    51601 cgatgtcagc ttaaatatcg tgccccagga atcaccgcta cttgaagagt ggctctcggc
    51661 ccagcgtcat gatttaggac tcactgagac actccatacg cctacgggaa cagaacgtac
    51721 cgaattactc tctttagatg aagtgtgtgt gttacctccg ggtcatccgc tggcggtaaa
    51781 aaaggtatta acgccggatg attttcaggg tgagaattac atcagccttt cccgtactga
    51841 cagctatcgc cagttgctgg atcagctatt tactgaacat caggttaaac gacgcatgat
    51901 cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta cgggcggggg taggtatttc
    51961 ggtggttaac ccgctcaccg cactagatta tgcggcaagc ggtttagtgg tgcggcggtt
    52021 cagtattgcg gttccgttca ccgtcagcct gatccgcccc ctgcaccgcc cgtcatcagc
    52081 gctggttcag gcgtttagtg ggcatttaca agcggggtta ccgaaactgg tcacttctct
    52141 tgacgctatt ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc ggcatggatc
    52201 gccgcggtat caaacacagg caaaacactg cgctcttctg gcaccagtaa accaatttct
    52261 gtgcagccaa aaatgacgcc ctgtgcgccc tgttctgcaa ggcgagcaat cacttgcgca
    52321 taataagcgc gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa aataatctga
    52381 ttaattttcg cccgttcatc cgcttcagga ataagacaat tgatggaaaa ttgttccgtc
    52441 agccgcccgc gataaaaatc ctgttccatg gtgtaacgcg tacccagcag cgccacacga
    52501 gtcattccgg ccccggtaat tgcacgtccg gtggcatccg caatgtgtaa gaaaggcaga
    52561 gtgcaacgtg actcaatggc atccgccact ttatgcatcg tattggtaca taacacaata
    52621 ccttctgcgc ccgcccgctg taagccaagc gccgcctcag ccagaatgtc cccggtttta
    52681 tcccattccc cgcgacgctg gcactcttct atttcatgaa aatcgacgct atgtagcagc
    52741 atttgcgcag agtgaagccc accaagccgc tgtttaatgc cttcatttat cagacgatag
    52801 taaggaatgg tggattccca gctcattcct cccagcaaac caattgtttt cattcctctc
    52861 tcctgatgtg tgttaccgca acaaggctga ataaggaacg agacaaacgc ctcaacggcc
    52921 aggtgcccaa tctctattaa cgaaaaaagg gccggatgta cagcacatcc ggcccgtgaa
    52981 atcagacgcc gatatttctc aacttctcgc ctgccatcag tttgcgttcg atatgttcca
    53041 gcgtgacatt tttggtttcc ggaatgagcc agaaagtaat acccacaaac gcaatgttca
    53101 gcgcagtgta gagccagaac gtaccggcag cgccaatgct atcaagcagt gtcaggaagg
    53161 ccgcgccgat aatcatattc gacacccagt ttgtcgtcgt cgaacaggta ataccgaaat
    53221 cgcggcattt cagcggctga atttcagagc acaggatcca caccactggc gcggcgctca
    53281 tcgcataacc ggcaatacac atcatcgtca tgccaacaga gagccaggac aagccactgg
    53341 aagccgtacc gttatcaaac tgcatcaggc aatagcccag caccagagtg cctaacgcca
    53401 tcacgctgaa accaattttc agagccggtt tacgacctgc tttatctacc gtaaagaccg
    53461 caatgaaggt cgcgaacata aaggtcagtc ccacgaccag agtcgcaatc atctgttgtt
    53521 ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc gtagtacatg atgatgttca
    53581 taccggtaaa ctgctgcatc gcctgcaaca acataccgag aaacacagca cgacggacgt
    53641 tacggttgat cttaaacagt gcccaaccgc cctgttttaa cttcaggctt tcacgaattt
    53701 cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag catacgcaac acttcttccg
    53761 cctcaatatg acgccccttt tccgccagcc agcgcgggct atttggcagg aagaccacca
    53821 gaataatcag cagaactgct ggtaaagcaa gaacccccaa cattgcgcgc cagttaccgc
    53881 tataactgaa cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg accatcaact
    53941 ggtacatact gatcatctta ccgcgaacgt tttcacttgc catttcagaa agatacagag
    54001 gagcggtgta agacgcgatc ccgacagcaa tgcccagcac cacacgagcg gcgattaaca
    54061 tctctacgct ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg atggcccccg
    54121 ccatcaggct gtatttacgc cccaggcgga acgacagcca accattaaac agcgcaccaa
    54181 ttgctgcgcc gagcatcatg ctactaacca cccattcctg caaacgactg gtcagcacaa
    54241 agtgatcggt aatgaacggc aacgctccgg cgattacgcc gatatcaaga ccaaataaca
    54301 atcctgcgac cgcagcagct accgaaacaa acatattcat acgccgcgta tcacgcaaag
    54361 aacgtggcgt taaagcagat tccgtattga tagtaaccat tttttcctgc cagcagagag
    54421 taagacattg tgaaaaaata cgtgaacaat tcacacaggt gtcaggtcgg aaacagcata
    54481 aatatggatt aaattgctgc gacatgccgt tatgtgatgg atattccaat tttaaaatta
    54541 agttgaatta ttgagattat tattaacctc ctaattttaa agcag
//
LOCUS       DAZXUL010000010        54418 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZXUL010000010 DAZXUL010000000
VERSION     DAZXUL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54418
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.010"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..911)
                     /gene="leuO"
                     /locus_tag="ACH3L1_000556"
     CDS             complement(<1..911)
                     /gene="leuO"
                     /locus_tag="ACH3L1_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFT4075442.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQW"
     gene            1570..1656
                     /gene="leuL"
                     /locus_tag="ACH3L1_000557"
     CDS             1570..1656
                     /gene="leuL"
                     /locus_tag="ACH3L1_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFT4075443.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            1749..3320
                     /gene="leuA"
                     /locus_tag="ACH3L1_000558"
     CDS             1749..3320
                     /gene="leuA"
                     /locus_tag="ACH3L1_000558"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFT4075444.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            3320..4411
                     /gene="leuB"
                     /locus_tag="ACH3L1_000559"
     CDS             3320..4411
                     /gene="leuB"
                     /locus_tag="ACH3L1_000559"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFT4075445.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            4414..5814
                     /gene="leuC"
                     /locus_tag="ACH3L1_000560"
     CDS             4414..5814
                     /gene="leuC"
                     /locus_tag="ACH3L1_000560"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFT4075446.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            5825..6430
                     /gene="leuD"
                     /locus_tag="ACH3L1_000561"
     CDS             5825..6430
                     /gene="leuD"
                     /locus_tag="ACH3L1_000561"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFT4075447.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(6479..7657)
                     /gene="setA"
                     /locus_tag="ACH3L1_000562"
     CDS             complement(6479..7657)
                     /gene="setA"
                     /locus_tag="ACH3L1_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFT4075448.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(7759..7890)
                     /gene="sgrT"
                     /locus_tag="ACH3L1_000563"
     CDS             complement(7759..7890)
                     /gene="sgrT"
                     /locus_tag="ACH3L1_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFT4075449.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            7979..9634
                     /gene="sgrR"
                     /locus_tag="ACH3L1_000564"
     CDS             7979..9634
                     /gene="sgrR"
                     /locus_tag="ACH3L1_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFT4075450.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      9668..9767
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9798..10781
                     /gene="thiB"
                     /locus_tag="ACH3L1_000565"
     CDS             9798..10781
                     /gene="thiB"
                     /locus_tag="ACH3L1_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFT4075451.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            10757..12367
                     /gene="thiP"
                     /locus_tag="ACH3L1_000566"
     CDS             10757..12367
                     /gene="thiP"
                     /locus_tag="ACH3L1_000566"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFT4075452.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            12351..13049
                     /gene="thiQ"
                     /locus_tag="ACH3L1_000567"
     CDS             12351..13049
                     /gene="thiQ"
                     /locus_tag="ACH3L1_000567"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFT4075453.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(13163..13927)
                     /locus_tag="ACH3L1_000568"
     CDS             complement(13163..13927)
                     /locus_tag="ACH3L1_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFT4075454.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(14013..14891)
                     /gene="araC"
                     /locus_tag="ACH3L1_000569"
     CDS             complement(14013..14891)
                     /gene="araC"
                     /locus_tag="ACH3L1_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFT4075455.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGKGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            15230..16930
                     /gene="araB"
                     /locus_tag="ACH3L1_000570"
     CDS             15230..16930
                     /gene="araB"
                     /locus_tag="ACH3L1_000570"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFT4075456.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            16941..18443
                     /gene="araA"
                     /locus_tag="ACH3L1_000571"
     CDS             16941..18443
                     /gene="araA"
                     /locus_tag="ACH3L1_000571"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFT4075457.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            18643..19338
                     /gene="araD"
                     /locus_tag="ACH3L1_000572"
     CDS             18643..19338
                     /gene="araD"
                     /locus_tag="ACH3L1_000572"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFT4075458.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            19413..21764
                     /gene="polB"
                     /locus_tag="ACH3L1_000573"
     CDS             19413..21764
                     /gene="polB"
                     /locus_tag="ACH3L1_000573"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFT4075459.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            21929..24835
                     /gene="rapA"
                     /locus_tag="ACH3L1_000574"
     CDS             21929..24835
                     /gene="rapA"
                     /locus_tag="ACH3L1_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFT4075460.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            24847..25506
                     /gene="rluA"
                     /locus_tag="ACH3L1_000575"
     CDS             24847..25506
                     /gene="rluA"
                     /locus_tag="ACH3L1_000575"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFT4075461.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(25623..26438)
                     /gene="djlA"
                     /locus_tag="ACH3L1_000576"
     CDS             complement(25623..26438)
                     /gene="djlA"
                     /locus_tag="ACH3L1_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFT4075462.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            26693..29047
                     /gene="lptD"
                     /locus_tag="ACH3L1_000577"
     CDS             26693..29047
                     /gene="lptD"
                     /locus_tag="ACH3L1_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFT4075463.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            29100..30386
                     /gene="surA"
                     /locus_tag="ACH3L1_000578"
     CDS             29100..30386
                     /gene="surA"
                     /locus_tag="ACH3L1_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFT4075464.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            30386..31375
                     /gene="pdxA"
                     /locus_tag="ACH3L1_000579"
     CDS             30386..31375
                     /gene="pdxA"
                     /locus_tag="ACH3L1_000579"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFT4075465.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            31372..32193
                     /gene="rsmA"
                     /locus_tag="ACH3L1_000580"
     CDS             31372..32193
                     /gene="rsmA"
                     /locus_tag="ACH3L1_000580"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFT4075466.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            32196..32573
                     /gene="apaG"
                     /locus_tag="ACH3L1_000581"
     CDS             32196..32573
                     /gene="apaG"
                     /locus_tag="ACH3L1_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFT4075467.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            32580..33422
                     /gene="apaH"
                     /locus_tag="ACH3L1_000582"
     CDS             32580..33422
                     /gene="apaH"
                     /locus_tag="ACH3L1_000582"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFT4075468.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(33500..33979)
                     /gene="folA"
                     /locus_tag="ACH3L1_000583"
     CDS             complement(33500..33979)
                     /gene="folA"
                     /locus_tag="ACH3L1_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFT4075469.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34170..36032)
                     /gene="kefC"
                     /locus_tag="ACH3L1_000584"
     CDS             complement(34170..36032)
                     /gene="kefC"
                     /locus_tag="ACH3L1_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFT4075470.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36025..36555)
                     /gene="kefF"
                     /locus_tag="ACH3L1_000585"
     CDS             complement(36025..36555)
                     /gene="kefF"
                     /locus_tag="ACH3L1_000585"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFT4075471.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36603..37997)
                     /locus_tag="ACH3L1_000586"
     CDS             complement(36603..37997)
                     /locus_tag="ACH3L1_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4075472.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(38056..38343)
                     /gene="fixX"
                     /locus_tag="ACH3L1_000587"
     CDS             complement(38056..38343)
                     /gene="fixX"
                     /locus_tag="ACH3L1_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFT4075473.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38340..39626)
                     /locus_tag="ACH3L1_000588"
     CDS             complement(38340..39626)
                     /locus_tag="ACH3L1_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFT4075474.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39677..40618)
                     /locus_tag="ACH3L1_000589"
                     /pseudo
     CDS             complement(39677..40618)
                     /locus_tag="ACH3L1_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(40633..41403)
                     /locus_tag="ACH3L1_000590"
     CDS             complement(40633..41403)
                     /locus_tag="ACH3L1_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFT4075475.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            41759..43273
                     /gene="caiT"
                     /locus_tag="ACH3L1_000591"
     CDS             41759..43273
                     /gene="caiT"
                     /locus_tag="ACH3L1_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFT4075476.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43304..44446
                     /gene="caiA"
                     /locus_tag="ACH3L1_000592"
     CDS             43304..44446
                     /gene="caiA"
                     /locus_tag="ACH3L1_000592"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFT4075477.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            44575..45792
                     /gene="caiB"
                     /locus_tag="ACH3L1_000593"
     CDS             44575..45792
                     /gene="caiB"
                     /locus_tag="ACH3L1_000593"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFT4075478.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            45866..47419
                     /gene="caiC"
                     /locus_tag="ACH3L1_000594"
     CDS             45866..47419
                     /gene="caiC"
                     /locus_tag="ACH3L1_000594"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFT4075479.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            47528..48313
                     /gene="caiD"
                     /locus_tag="ACH3L1_000595"
     CDS             47528..48313
                     /gene="caiD"
                     /locus_tag="ACH3L1_000595"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFT4075480.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            48319..48909
                     /gene="caiE"
                     /locus_tag="ACH3L1_000596"
     CDS             48319..48909
                     /gene="caiE"
                     /locus_tag="ACH3L1_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFT4075481.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(48995..49390)
                     /gene="caiF"
                     /locus_tag="ACH3L1_000597"
     CDS             complement(48995..49390)
                     /gene="caiF"
                     /locus_tag="ACH3L1_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFT4075482.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(49652..52873)
                     /gene="carB"
                     /locus_tag="ACH3L1_000598"
     CDS             complement(49652..52873)
                     /gene="carB"
                     /locus_tag="ACH3L1_000598"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFT4075483.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(52891..54039)
                     /gene="carA"
                     /locus_tag="ACH3L1_000599"
     CDS             complement(52891..54039)
                     /gene="carA"
                     /locus_tag="ACH3L1_000599"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFT4075484.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
BASE COUNT        13173 a        14724 c        14181 g        12340 t
ORIGIN      
        1 atccactgat ggcctttatc gcgcccggca gcttcatgcc aagagagata acaggttctg
       61 ctgttttgtt ttaacggcag cggtaatacc tgtaattcta aggattcagc gaactcttca
      121 gccagccaac gcggcgcaat agcgaccaaa tgcgtttgcg acaccacgct aagtacgctc
      181 atcattgcca tgccctgata cgcgatactg gcttgcttat ctaccgtgtc ataccaaggt
      241 tgactaaatg acgcgaaacg atcgagcgaa accgccgcat gttgttcgtt ataaacatca
      301 tgtttcagta acgggccttt aattgttgga tgatttttgc tggctaccag caccatttca
      361 tctttaaata atggcacgct ggtaaattca ggacgatgga agtcttcata actaatcaca
      421 aactccgttt cctgataacg cagctgatgt tcagtgttct gatttaatga agacttgaac
      481 ataacatgta tatttggcgc aatctgctca atgtgattat aaatctgcga ggtcagaatg
      541 ctgtctaacg ggctgcaaac acaaagatga aatacacgtt cactgctcgc gggttcaaaa
      601 cctgaaccag gcaattcatt ttgtactagt tgcaatgcct ggcgaactga accaaaaagt
      661 tgaaatgcgc gagcagtcgg ttgaatacca cggccataac gaacaaaaag ctcgtcatta
      721 aacatcacct tcaggcgtgc aacagcgtta ctgaccgcag gttgcgacat acccagaaca
      781 tgagcggcac gggtaatgtt ttgctcctgc atcacggcat cgaaaacggt taataagttg
      841 agatcgacca tgcgtagctg tggcttgctt aactccgccg tctctgaatg atctgtttgt
      901 acctctggca tacttaactc cactgtcaca cttaactccc tttcccttat tggaatgcag
      961 agaaataatc ctgaagaata tgatttatca tgcatataaa ataagaaaaa gcaaaatgag
     1021 taaaattcgg aaaacataaa gatacgggtt ctgataaaaa cagaatccat tgccgtggaa
     1081 tgagtcattt accacagcat aataatccat aatgttttgt tgcgaaaaca atctaatcat
     1141 aactaccgcg aatactcaat catctacaaa atggattaaa tgtgcttatt taataattaa
     1201 tttatatatt taatgcatta attcttaaca ttaattgttc aataatattc accaaatcaa
     1261 tatcaaaaaa aatcgcaaaa catataattc aatacaaatc atcaggatag gttttgcaac
     1321 gcgtgcattt tgtccccttt ttcctcgttg attagatgca aaaatttatg ctgaaatatg
     1381 tcaaccgatg aaaagcgtcg gtagttaagc agaaattaat atcgcttact ttaaccaccg
     1441 tagcacaatt agctaatttt acggatgcag aactcacgct ggcgggacgt ttttattgcg
     1501 tcagggttga catccgtttt tgtatccagt aactctaaaa gcatatcgca ttcatctgga
     1561 gctgatttaa tgactcacat cgttcgcttt atcggtctac tactactaaa cgcatcttct
     1621 ttgcgcggta gacgagtgag cggcatccag cattaagcca gcacgcagtc aaacaaaaaa
     1681 cccgcgccat tgcgcgggtt tttttatgcc cgaagcgagg cgctctaaaa gagacaagga
     1741 cccaaaccat gagccagcaa gtcattattt tcgataccac attgcgcgac ggtgaacagg
     1801 cgttacaggc aagcttgagt gtgaaagaaa aactgcaaat tgcgctggcc cttgagcgta
     1861 tgggtgttga cgtgatggaa gtcggtttcc ccgtctcttc gccgggcgat tttgaatcgg
     1921 tgcaaaccat cgcccgccag gttaaaaaca gccgcgtatg tgcgttagct cgctgcgtgg
     1981 aaaaagatat cgacgtggcg gccgaatccc tgaaagtcgc cgaagccttc cgtattcata
     2041 cctttattgc tacctcccca atgcacatcg ccaccaagct gcgcagcacc ctagacgagg
     2101 tgatcgaacg cgctatctat atggtgaaac gcgcccgtaa ttacaccgat gatgttgaat
     2161 tttcttgcga agatgccggg cgtacaccca ttgccgatct ggcgcgagtg gtcgaagcgg
     2221 cgattaatgc cggtgccacc accatcaaca ttccggacac cgtgggctac accatgccgt
     2281 ttgagttcgc cggaatcatc agcggcctgt atgaacgcgt acctaacatc gacaaagcca
     2341 ttatttccgt acatacccac gacgatttag gcctggcggt cggcaactca ctggcggcgg
     2401 tacatgccgg tgcacgccag gtggaaggtg caatgaacgg gatcggcgag cgtgccggta
     2461 actgttcgct ggaagaagtc atcatggcga ttaaagttcg taaggatatt ctcaacgtcc
     2521 acaccgccat taatcaccaa gagatatggc gcaccagcca gttagttagc cagatttgta
     2581 atatgccgat cccggcaaac aaagccattg ttggcagcgg cgcattcgct cactcctccg
     2641 gtatccacca ggatggcgtg ctgaaaaacc gcgaaaacta cgaaatcatg acaccagaat
     2701 ctattggtct gaaccaaatc cagctgaatc tgacctctcg ttcggggcgt gcggcggtga
     2761 aacatcgcat ggatgagatg gggtataaag aaagtgaata taatttagac aatttgtacg
     2821 atgctttcct gaagctggcg gacaaaaaag gtcaggtgtt tgattacgat ctggaggcgc
     2881 tggccttcat cggtaagcag caagaagagc cggagcattt ccgtctggat tacttcagcg
     2941 tgcagtctgg ctctaacgat atcgccaccg ccgccgtcaa actggcctgt ggcgaagaag
     3001 tcaaagcaga agccgccaac ggtaacggtc cggtcgatgc cgtctatcag gcaattaacc
     3061 gcatcactga ctataacgtc gaactggtga aatacagcct gaccgccaaa ggtcacggta
     3121 aagatgcgct gggtcaggtg gatattgtca ccaactacaa cggtcgccgc ttccacggcg
     3181 tcggcctggc caccgatatt gtcgagtcct ccgccaaagc catggtgcac gtactgaaca
     3241 atatctggcg tgccgcagaa gtcgaaaaag agttgcaacg caaaactcaa cacaacgaaa
     3301 acaacaagga aaccgtgtga tgtcgaagaa ttaccatatt gccgtattgc cgggagacgg
     3361 tattggtccg gaagtgatga cccaggcgct gaaagtgctg aatgccgtgc gcaaccgctt
     3421 tgcgatgcgc atcaccacca gccattacga tgtaggcggc gcagccattg ataaccacgg
     3481 gcaaccactg ccgcctgcga cggttgaagg ttgtgagcaa gccgatgccg tgctgtttgg
     3541 ctcggtaggc ggcccgaagt gggaacattt accaccagac cagcaaccag aacgcggcgc
     3601 gctgctgccg ctgcgtaagc acttcaaatt attcagcaac ctgcgcccgg caaaactgta
     3661 tcaggggctg gaagcattct gtccgctgcg tgcagacatt gccgctaacg gcttcgacat
     3721 ccagtgcgtg cgcgaactga ccggcggcat ctatttcggt cagccaaaag gccgcgaagg
     3781 tagcggacaa tatgaaaaag cgtttgatac cgaggtgtat caccgttttg agatcgagcg
     3841 tattgcccgc atcgcgtttg aatctgctcg taaacgtcgc cacaaagtca cctcaatcga
     3901 caaagctaac gtgctgcaat cctctatttt atggcgtgaa atcgtcaacg agatcgccac
     3961 ggaatacccg gatgtcgaac tggcgcatat gtacatcgac aacgccacca tgcagctgat
     4021 taaagatcca tcacagtttg acgttctgct gtgctccaac ctgtttggcg acattctgtc
     4081 tgacgagtgc gcaatgatca ctggctcgat gggcatgttg ccttccgcca gcctgaacga
     4141 gcaaggtttt ggactgtatg aaccggcggg cggctcggca ccagatatcg caggcaaaaa
     4201 catcgccaac ccgattgcac aaatcctgtc gctggcactg ctgctgcgtt acagcctgga
     4261 tgccgatgat gcggcttccg ccattgaacg cgccattaac cgtgcattag aagaaggcat
     4321 tcgcaccggg gatttagccc gtggcgctgc cgccgttagt accgatgaaa tgggcgatat
     4381 cattgcccgc tatgtggcag aaggggtgta atcatggcta aaacgttata cgaaaaatta
     4441 ttcgacgctc acgtagtgta cgaagccgaa aacgaaaccc cgctgttata tatcgaccgc
     4501 cacctggtgc atgaagtgac ctcaccgcag gcgttcgatg gtctgcgcgc ccacggtcgc
     4561 ccggtacgtc agccgggcaa aaccttcgcc actatggatc acaacgtctc tacccagacc
     4621 aaagacatta atgcctgtgg tgaaatggcg cgcatccaga tgcaggagct gattaaaaac
     4681 tgcaaagaat ttggcgtcga gctgtatgac ctgaatcacc cgtatcaggg gatcgtccac
     4741 gtaatggggc cggaacaggg cgtcaccttg ccggggatga ccattgtctg cggcgactcg
     4801 cataccgcca cccacggcgc gtttggcgca ctggcctttg gtatcggcac ttccgaagtt
     4861 gaacacgtac tggcaacaca aaccctgaaa cagggccgtg cgaagaccat gaaaattgaa
     4921 gtccagggca aagccgcgcc gggcattacc gcaaaagata tcgtgctggc aattatcggt
     4981 aaaactggca gcgcaggcgg caccgggcat gtggtggagt tttgcggcga agcaattcgt
     5041 gatttaagca tggaaggtcg tatgaccctg tgcaatatgg cgatcgaaat gggcgcaaaa
     5101 gccgctctgg ttgctcctga cgaaaccacc tttaactatg tcaaaggccg tctgcatgcg
     5161 ccgaaaggca aagatttcga cgacgccgtt gcctactgga aaaccctgca aaccgacgaa
     5221 ggcgcaactt tcgataccgt tgtcactctg caagcagaag aaatttcacc gcaggtcacc
     5281 tggggcacca atcccggcca ggtgatttct gtgaacgaca atattcccga tccggcttcg
     5341 tttgccgatc cggttgaacg tgcgtcggca gaaaaagcgc tggcctatat ggggctgaaa
     5401 ccgggtattc cgctgaccga agtggctatc gacaaagtgt ttatcggttc ctgtaccaac
     5461 tctcgcattg aagatttacg cgcggcagcg gagatcgcca aagggcgaaa agtcgcgcca
     5521 ggcgtgcagg cactggtggt tcccggctct ggcccggtaa aagcccaggc ggaagcggaa
     5581 ggtctggata aaatatttat tgaagccggt tttgaatggc gcttgcctgg ctgctcaatg
     5641 tgtctggcga tgaacaacga ccgtctgaat ccgggcgaac gttgtgcatc caccagcaac
     5701 cgtaactttg aaggccgcca ggggcgcggc gggcgcacgc atctggtcag cccggcaatg
     5761 gctgccgctg ctgctgtgac cggacatttc gccgacattc gcaacattaa ataaggagca
     5821 caccatggca gagaaattta tcaaacacac aggcctggtg gttccgctgg atgccgccaa
     5881 tgtcgatacc gatgcaatca tcccgaaaca gtttttgcag aaagtgaccc gtacgggttt
     5941 tggcgcgcat ctgtttaacg actggcgttt tctcgatgaa aaaggccaac agccaaaccc
     6001 ggacttcgtg ctgaacttcc cgcagtatca gggcgcttcc attttgctgg cacgagaaaa
     6061 cttcggctgt ggctcctcgc gtgagcacgc gccctgggca ttgaccgact acggttttaa
     6121 agtggtgatt gcgccgagtt ttgctgacat cttctacggc aatagcttta acaaccagct
     6181 gctgccggtg aaattaagcg atgcagaagt ggacgaactg tttgcgctgg tgaaagccaa
     6241 tccggggatc catttcgacg tggatctgga agcgcaagag gtgaaagcgg gagagaaaac
     6301 ctatcgcttt accatcgatg ccttccgccg ccactgcatg atgaatggtc tggacagcat
     6361 cggcctgacc ctgcagcacg acgacgctat tgcgtcttat gaagcaaaac aacctgcgtt
     6421 tatgaattag accccttgcc cggtcaaatg accgggcttt ccgctatcgt ccacgtcatc
     6481 aaacgtcttt aacctttgcg gttaaaaata atgcgacaac agaaataatc gcaattaccc
     6541 agtagacagc aaagtgcccc caactttgtg caattgctcc ctgaataaca ccagccagaa
     6601 ttaccccggt agagatagtg ttggtaaata aggtggtagc tgcccccgct cttccaggca
     6661 ttaaatcctg aaaccatagc atcccaatac ccgcaacaat gccgataaat acagcgttaa
     6721 aaagttgcag cgtcatcaac gccatacggc tatgaaagaa aatcaatcca gtgtaaaaca
     6781 gcactcctgc caccactgca atgaccatca ttcgccgctt accataacgt ttgacatagt
     6841 agccagccag aatcattgct ggtatttcca gtccagctgc cgtccccatc aggaaacccg
     6901 ccagtttgtc tggcaatcct aaatcgctac tgatccacaa tggcatatca ataatgtaca
     6961 tagtgttgca ggtccacatt aacgtcgagg cgacaaataa catccgtacg ttactatcct
     7021 gccagtcacc ttgcattgat aaagcatttt ccgacggcag ttctacccgc gccacagaca
     7081 gaagcataaa tgcaatcaat accagactga gtgtgaatat cccggcggca atcgaaaaca
     7141 tcactgtaaa gccgtaattc aacgccagca taaaggccag cggtggaccg attacccatg
     7201 ccagagaaag ctgcgcacgc atcaccgagc taaacatcac cacttctcgc gccgagttat
     7261 ccgcgtattc ccgcgccaga gcaaataact gtggcattgc cgtattggcc agagatgcca
     7321 gaagcacacc acaggtgata agcgtcagat aatgacgatt aaatgcaaac agtagcgcat
     7381 tgccaatagc catcaaacag caaaatataa tcagttttcg ccgatcgccc tgactgtcag
     7441 aacgttttgc caaccagagg cttacgccga tcccagcaat agcattcacc gtataaaaga
     7501 gtccgatcca gaaaggttgc gcgccaacct cacgactcag aaataagctc aatgtaggag
     7561 cctgtagcgc cccggccacc cccatcataa aagcgaccag cataaatgcc gcgtaaacac
     7621 cgttcatacg gcgagccatc gtcattatcc agatcatacg ttcccttttt agcgcggcga
     7681 gaataaaaaa aaccagcagg tataatctgc tggcgggtga ttttacacca atactcagtc
     7741 acacatgatg caggcaagtc aactttcaga attgcggtca tcccactgca tcagtccttc
     7801 aagcatggtt aatcgttgtg ggacgcttaa ccaacgcaac cagcacaact tcgctgtcgc
     7861 ggtaaaatag tgctgataaa actgacgcat ggggcacctc cttgcttcat cgttaggaat
     7921 tattgactta atatagggaa aataaaattg ctgtcttttg cgcaggagtt ccccttttat
     7981 gccatctgct cgtctgcaac aacagttcat ccgcctgtgg caatgctgcg agggtaaatc
     8041 gcaggacaca acgctcaacg aactggcagc gttattgagc tgctcgcgtc gtcatatgcg
     8101 taccctgctc aacaccatgc aggatcgcgg ctggctgacg tgggaagcgg aagtcgggcg
     8161 cggtaaacgc tcgcgtctga cattcctcta taccgggctg gcgcttcagc aacagcgggc
     8221 ggaagacctg ctggagcagg atcgtatcga ccaactagtg cagttggttg gcgacaaagc
     8281 gactgtgcgg caaatgctgg tttctcatct gggccgcagc ttccgccagg ggcggcacat
     8341 cctgcgcgtg ctctactatc gtccgttgcg taatctgcta cctggcagcg cattgcgccg
     8401 ttccgaaacc catattgccc ggcaaatctt cagttcgcta acgcgcataa atgaggaaaa
     8461 tggggaactg gaagcagaca tcgcccacca ctggcagcaa atatcaccgc ttcactggcg
     8521 tttctttttg cgtccaggag tccattttca tcatggtcgt gaactggaaa tggacgacgt
     8581 gatcgcctct ttaaaacgaa tcaatacgct gccgctctat tcgcatatta cggacattgt
     8641 gtcgccgacg ccctggacgc tggatatcca tctcacgcag ccggaccgct ggttgccgtt
     8701 actgctgggg caagttccgg cgatgatcct gccgtgcgaa tgggaaaccc tcagtaactt
     8761 tgccagccat cctattggca ccggtccgta tgcggtgatt cgcaacagca ccaatcaact
     8821 gaaaattcag gcattcgatg acttcttcgg ttaccgggca ttaatcgacg aagttaacgt
     8881 ctgggttctg ccggaaattg ccgacgagcc agccggtggt ctgatgctaa aaggtccaca
     8941 gggcgagaaa aaagagattg aaagccgcct ggaggaaggt tgctactatt tactgttcga
     9001 cagccgcacc catcgcgggg cgaatcagca agtcagggac tgggtaagct atgtgctttc
     9061 tccaactaat ctggtctatt tcgctgagga acagtaccag caactgtggt tcccggctta
     9121 tggactgctc ccccgttggc accatgcccg caccataaag agcgaaaaac cggctggcct
     9181 ggaaagcctc accctgacct tttatcagga tcacagtgag catcgggtga ttgccgggat
     9241 catgcagcag attctggcaa gtcaccaggt cacgctggaa atcaaagaga tcagctacga
     9301 tcagtggcat gaaggagaga tcgagagcga tatctggctt aatagcgcca actttacgct
     9361 gccgctggat ttttcgctgt tcgcgcactt gtgcgaggtg ccgctgctcc aacactgtat
     9421 tccgatcgac tggcaagccg acgccgcccg ctggcgcaat ggcgaaatga acctggcgaa
     9481 ctggtgccag caactggtcg ccagtaaagc aatggtgccg cttatccacc actggctgat
     9541 cattcaggga caacgcagta tgcgcggcct gcgtatgaat accctcggct ggttcgattt
     9601 taaatcagcg tggtttgcgc caccggatcc atgattgctg gtagataaca aaatcactac
     9661 actaacgccg ttctcaacgg ggtgccacgc aaacgcgtgc gctgagaaaa tacccgtcga
     9721 acctgatccg gataacgccg gcgaagggat ttgaggctcc ttctcaagtc ctttgccact
     9781 cttttttgag gtgcaaagtg ttaaaaaaat gtcttcccct gctgttgctg tgcacagcgc
     9841 ccgttttcgc taaacccgtt ctgactgttt atacctacga ttccttcgcc gccgactggg
     9901 ggcctggtcc aaagatcaaa aaagcttttg aagccaactg taactgcgaa ctgaaactgg
     9961 tggcgctgga agatggcgtt tcgcttctca accgtctacg gatggaaggc aaaaatagta
    10021 aagccgatgt ggtgctgggg ctggataaca atctgttaga cgccgccagt aaaaccggac
    10081 tatttgccaa aagcggtgtg gcagcggatg ccgttaacgt tcccggcggc tggaataatg
    10141 acactttcgt cccgtttgat tacggctatt tcgccttcgt ctatgacaag aacaaactga
    10201 aaaacccgcc gcaaagcctg aaagaattgg ttgagagcga tcagaactgg cgggtgattt
    10261 atcaggatcc gcgtaccagc acgccggggc tgggtctgct gttgtggatg caaaaagtct
    10321 atggcgatga cgccccacaa gcctggcaaa aactggcgaa gaaaaccgtc acggtcacca
    10381 aaggctggag cgaagcctac ggcctgtttt taaaaggtga aagcgatctg gtactgagtt
    10441 acaccacctc tccggcttat cacattctcg aagagaaaaa agataactac gccgccgcga
    10501 actttagcga aggtcactat ctgcaggtgg aagtcgccgc ccgcaccgct gccagcaagc
    10561 agccggagct ggcgcaaaaa ttcctccagt ttatggtttc tccggctttt cagaatgcga
    10621 tcccaaccgg taactggatg tatccggtgg caaatgtcac gctgcctgcc gggtttgaac
    10681 aattgaccaa accggccacc acgctggagt tcacaccagc tgaagtggcg gcacaacgtc
    10741 aggcatggat tagcgaatgg caacgcgccg tcagccgtta attcccggct ggttaattcc
    10801 aggtgtaagc gccgccacgc tggtggtagc ggttgcgctc gcggcgtttc tcgccctgtg
    10861 gtggaacgcg ccgcagggta actgggtggc agtctggcag gacagctacc tgtggcatgt
    10921 ggtgcgcttc tccttctggc aggcgtttct ctcggcgcta ctctctgtcg tacccgcgat
    10981 attcctcgcc cgcgccctct atcgcaggcg ctttccgggt cggctggcgc tgttgcgtct
    11041 gtgcgcaatg accttgatcc tcccggtgct agttgctgtt ttcggcattc ttagcgtcta
    11101 tggtcgtcag ggctggctgg catcgctctg ccaatcgctc ggtctggagt ggaccttttc
    11161 gccctacggc ctgcaaggca ttttgctggc gcacgtattt tttaatctgc cgatggcgag
    11221 ccgcttatta ctccaggcac tggaaaacat tcccggcgaa caacgtcagc ttgccgccca
    11281 gcttgggatg cgcggctggc attttttccg cttcgtcgaa tggccgtggc tacggcgaca
    11341 aatcccgccg gttgccgcgc ttatctttat gctctgtttc gccagcttcg ccaccgtgct
    11401 atcgctgggc ggcggtccgc aggcaaccac tatcgagctg gctatttatc aggcgctgag
    11461 ttacgactac gatcctgccc gcgcggcgat gctggcgctg ctccagatgg tgtgttgcct
    11521 tgggctggtg ctgttgagtc agcgactaag taaggccatt gcgcccggca ccacgctgct
    11581 gcaaggctgg cgcgatccgg acgatcgtct gcatagccgc atttgcgaca cggtgttaat
    11641 agtgctggcg ctgctgctgt tgctgccacc gttgctggcg gtgatcgtcg atggggtaaa
    11701 tcgccagttg ccggaagtgc tggcacaacc ggtgctgtgg caggcgctgt ggacctcgtt
    11761 gcgtattgcg ctggcggcag gtgtattgtg cgtagtgctg accatgatgc tgctatggag
    11821 cagtcgcgaa ctgcgggcgc ggcagaaaat gctggcgggt caggcgctgg agatgagcgg
    11881 catgttgatc ctcgccatgc cggggattgt gctggctacc ggcttctttt tactgctcaa
    11941 caacactatc ggcctgccac aatctgctga cggcattgtg attttcacca atgcgttaat
    12001 ggcgatccct tatgcgctga aagtactgga aaacccgatg cgcgatatca ccgcccgcta
    12061 cagtatgttg tgtcagtcgc tgggcattga aggctggtcg cgcttaaaag tggtcgagct
    12121 gcgcgccctg aaacgtccac tggcgcaggc gctggctttt gcctgcgtgc tgtcgattgg
    12181 tgattttggc gtagtggcgt tgttcggtaa cgatgatttc cgtaccctgc cgttttatct
    12241 ctaccagcaa attggctcct atcgcagcca ggacggcgcg gtcaccgcgt taattctgct
    12301 actactctgt tttctgctgt ttaccgtgat tgaaaaacta ccggggcgaa atgttaaaac
    12361 tgactgatat cacctggctt tatcaccatt tgccgatgcg ttttagctta acggtagaac
    12421 gcggcgagca ggtggcaatc ctcgggccaa gcggcgcggg taaaagtacc ctgctgaatt
    12481 tgattgccgg ttttctgacg ccagccagcg gttcgctgac tatcgatggt gttgatcaca
    12541 ccacgacgcc gccgtcacgc cgtccggtgt cgatgctgtt tcaggagaac aacctgttca
    12601 gccatctgac ggtcgcgcag aacatcgggc tggggctaaa tccggggttg aaactgaacg
    12661 cggcacagca ggagaaaatg cacgcgattg cccgtcagat ggggattgat aatttaatgg
    12721 cgcggttacc gggcgagctt tccggcggtc agcgacagcg cgtggcgtta gcgcgttgtc
    12781 tggtgcgcga acagccgatt ttattgctcg atgaaccgtt ctctgcgctc gatccggcgt
    12841 tacgtcagga gatgctgacg ctggtgagca cgagctgcca gcagcaaaaa atgacgctat
    12901 tgatggtgtc gcacagtgtg gaagatgcgg cgcggatcgc cacacgctcg gtggtggtcg
    12961 ccgacgggcg catcgcctgg cagggtaaga ctgaagaatt gttgagcggt aaggcgagtg
    13021 cttcggcaat attggggatt acgggttagt atgcactgtc agatgcggcg tgaacacctt
    13081 atccgaccta cggacagtgc gttttgtagg cctgataaga cgcggtcagc gtcgcatgag
    13141 gcaacccgca caagacaatc ccctaaaccc caaccacttt acgcaaaata tcgatataca
    13201 ccggcatcaa cgggtggcga attaacacca ccagcgccac cacgccgatg gcagaaatca
    13261 acggcgtcag ccacaacaaa cggccgcgcg acaaataatg acttaaacga tcggttgctt
    13321 taccgctgcg ccataaccgc cagcacagcc agccaccaac ccacaaaaat accgccgtcg
    13381 ccagcagcaa ccatttaaac tcgccgctct gcattccggc tggaatatcg atcgccgcgc
    13441 ccgccagaat cccaggcagg aagtaaaacg gtggccacag caggcagccg ataatattcg
    13501 gcgtaatgaa ttttgccacc ggcagatcca gcattcccgc caccattggc accagcggac
    13561 gcgtcgggcc aacaaagcga ccgaccagaa tggtgaacat gctgtgttga tgcaacgcat
    13621 gttcagtttt atcgagtaat gctttgtttt tcttcagaaa tgaccagcga tgcaacggct
    13681 ttttaaaacg ccagcccagc cagaaggaga tccagtcgcc cagcagacaa ccgacaatcc
    13741 ctgccagcca agcgtgccag aaacttaact cgccgctgcc aatcagcgct cccagccccg
    13801 ccatcagcac cgtaccgggt agaatcaaac cgaccagcgc cagcgactcc agaaaggcca
    13861 ccaacaccac cgccatcaat gaatacacgg tagattgggt aataaagtgt tccagcaatg
    13921 cttgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct atgctactcc
    13981 gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc
    14041 actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat
    14101 acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc
    14161 atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt
    14221 aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa
    14281 acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac
    14341 tgacaagcct cgcgtacacg attatccatc ggtggatgga gcgactcgtt aatcgcttcc
    14401 atgcgccgca gtaacaattg ctcaagcaga ttcatcgcca gcagctccga atagcgccct
    14461 ttcccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatacgg ctggtgcgct
    14521 tcatccgggc gaaagaaacc cgtattggca aatattgacg gccagttaag ccattcatgc
    14581 cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga
    14641 caaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcagaca
    14701 aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa
    14761 cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc
    14821 gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc
    14881 gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc
    14941 atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccca accggtaacc
    15001 ccgcttatta aaagcattct gtaacaaagc aggaccaaag ccatgacaaa aacgcgtaac
    15061 aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt
    15121 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg
    15181 caactctcta ctgtttctcc atacccgttt ttttggatgg agtgaaacga tggcgattgc
    15241 aattggcctc gattttggca gtgattctgt gcgagctttg gcggtggact gcgccagcgg
    15301 tgaagagatc gccaccagcg tagagtggta tccccgttgg caaaaagggc aattttgtga
    15361 tgccccgaat aaccagttcc gtcatcatcc gcgtgactac attgagtcaa tggaagcggc
    15421 actgaaaacc gtgcttgcag agcttagcgt cgaacagcgc gcagctgtgg tcgggattgg
    15481 cgttgacagt accggctcga cgcccgcacc gattgatgcc gacggtaacg tgctggcgct
    15541 gcgcccggag tttgccgaaa acccgaacgc gatgttcgta ttgtggaaag accacactgc
    15601 ggttgaagaa gcggaagaga ttacccgttt gtgccacgcg ccgggcaacg ttgactactc
    15661 ccgctatatt ggcggtattt attccagcga atggttctgg gcaaaaatcc tgcatgtgac
    15721 tcgccaggac agcgccgtgg cgcaatctgc cgcatcgtgg attgagctgt gcgactgggt
    15781 gccagctctg ctttccggta ccacccgccc gcaggatatt cgtcgcggac gttgcagcgc
    15841 cgggcataaa tctctgtggc acgaaagctg gggcggcttg ccgccagcca gtttctttga
    15901 tgagctggac ccgatcctca atcgccattt gccttccccg ctgttcactg acacctggac
    15961 tgccgatatt ccggtgggca ccttatgccc ggaatgggcg cagcgtctcg gcctgcctga
    16021 aagcgtggtg atttccggcg gcgcgtttga ctgccatatg ggcgcagttg gcgcaggcgc
    16081 acagcctaac gcactggtaa aagttatcgg tacttccacc tgcgacattc tgattgccga
    16141 caaacagagc gttggcgagc gggcagttaa aggtatttgc ggtcaggttg atggcagcgt
    16201 ggtgcctgga tttatcggtc tggaagcagg ccaatcggcg tttggtgata tctacgcctg
    16261 gtttggtcgc gtactcggct ggccgctgga acagcttgcc gcccagcatc cggaactgaa
    16321 agcgcaaatc aacgccagcc agaaacaact gcttccggcg ctgaccgaag catgggccaa
    16381 aaatccgtct ctggatcacc tgccggtggt gctcgactgg tttaacggtc gtcgcacgcc
    16441 aaacgctaac caacgcctga aaggggtgat taccgatctt aacctcgcta ccgacactcc
    16501 gctgctgttc ggcggtttga ttgctgccac cgcctttggc gcacgcgcaa tcatggagtg
    16561 ctttaccgat caggggatcg ccgtcaataa cgtgatggcg ctgggcggca tcgcgcggaa
    16621 aaaccaggtc attatgcagg cctgctgcga cgtgctgaat cgcccgctgc aaattgttgc
    16681 ctctgaccag tgctgtgcgc tcggtgcggc gatttttgct gccgtcgccg cgaaagtgca
    16741 cgcagacatc ccatcagccc agcaaaaaat ggccagtgcg gtagagaaaa ccctgcaacc
    16801 gcgcagcgaa caggcacaac gctttgaaca gctttatcgc cgctatcagc aatgggcgat
    16861 gagcgccgaa caacactatc ttccaacttc cgccccggca caggctgccc aggccgttgc
    16921 gactctataa ggacacgata atggcgattt ttgataatta tgaagtgtgg tttgtcattg
    16981 gcagccagca tctgtatggc ccggaaaccc tgcgtcaggt cacccaacat gccgagcacg
    17041 ttgttaatgc gctgaatacg gaagcgaaac tgccctacaa actggtgttg aaaccgctgg
    17101 gcaccacgcc ggatgaaatc accgctattt gccgcgacgc gaattacgac gatcgttgcg
    17161 ctggtctggt ggtgtggctg cacaccttct ccccggccaa aatgtggatc aacggcctga
    17221 ccatgctcaa caaaccgttg ctgcaattcc acacccagtt caacgcggcg ctgccgtggg
    17281 acagtatcga tatggacttt atgaacctga accagactgc acatggcggt cgcgagttcg
    17341 gcttcattgg cgcgcgtatg cgtcagcaac atgccgtcgt taccggtcac tggcaggata
    17401 aacaagccca tgagcgtatc ggctcctgga tgcgtcaggc ggtctctaaa caggataccc
    17461 gtcatctgaa agtctgccga tttggcgata acatgcgtga agtggcggtc accgatggcg
    17521 ataaagttgc cgcacagatc aagttcggtt tctccgtcaa tacctgggcg gttggctatc
    17581 tggtgcaggt ggtgaactcc atcagcgacg gcgatgttaa cgcgctggtc gatgagtacg
    17641 aaagctgcta caccatgacg cctgccacac aaatccacgg cgaaaaacga cagaacgtgc
    17701 tggaagcggc gcgtattgag ctggggatga agcgtttcct ggaacaaggt ggcttccacg
    17761 cgttcaccac cacctttgaa gatttgcacg gtctgaaaca gcttccaggt ctgcccgtac
    17821 agcgtctgat gcagcagggt tacggctttg cgggcgaagg cgactggaaa accgccgccc
    17881 tgcttcgcat catgaaggtg atgtcaaccg gtctgcaggg cggcacctcc tttatggagg
    17941 actacaccta tcacttcgag aaaggtaatg acctggtgct cggctcccat atgctggaag
    18001 tctgcccgtc gattgccgta gaagagaaac cgatcctcga cgttcagcat ctcggtattg
    18061 gtggtaagga cgatcctgcc cgactgatct tcaataccca aaccggtcca gcgattgtcg
    18121 ccagcctgat tgatctcggc gatcgttacc gtctgctggt taactgtatc gacacggtga
    18181 aaacaccgca ctccctgccg aaactgccgg tggcgaatgc actgtggaaa gcgcaaccgg
    18241 atctgccaac tgcttccgaa gcgtggatcc tcgctggtgg cgcgcaccat accgtcttca
    18301 gccatgcgct gaacctcaac gatatgcgcc agttcgccga gatgcacgac attgaaatca
    18361 cggtgattga taacgacacc cgcctgccag cgtttaaaga cgcactgcgc tggaacgaag
    18421 tgtattacgg atttcgtcgc taagtagcca catcaggtat gtaacgcctg atgcgacgct
    18481 gacgcgtctt atctggccta cacgctgcga ttttgtaggc cggataagca aagcgcatcc
    18541 ggcattcaac gcctgatgcg acgctggcgc gtcttatcag gcctacgcgc tgcgattttg
    18601 tagaccggat aagcgaagcg catccggcac gaaggagtca acatgttaaa agatctcaaa
    18661 cgccaggtat tagaagccaa cctggcgctg ccaaaacaca acctggtcac gctcacatgg
    18721 ggcaacgtca gcgccgttga tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc
    18781 gattacagcg tcatgaccgc tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg
    18841 gttgaaggta cgaaaaagcc ctcctccgac acgccaactc accggctgct ctatcaggca
    18901 ttcccctcca ttggcggcat tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag
    18961 gcgggtcagt cgattccagc aaccggcacc acccacgccg actatttcta cggcaccatt
    19021 ccctgcaccc gcaaaatgac cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt
    19081 aacgtcatcg tagaaacctt tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt
    19141 ctggtccatt cccacggccc gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac
    19201 gccatcgtgc tggaagaggt cgcttatatg gggatattct gccgtcagtt agcgccgcag
    19261 ttaccggata tgcagcaaac gctgctggat aaacactatc tgcgtaagca tggcgcgaag
    19321 gcatattacg ggcagtaatg actgtataaa accacagcca atcaaacgaa accaggctat
    19381 actcaagcct ggttttttga tggattttca gcgtggcgca ggcaggtttt atcttaaccc
    19441 gacactggcg ggacaccccg caagggacag aagtctcctt ctggctggcg acggacaacg
    19501 ggccgttgca ggttacgctt gcaccgcaag agtccgtggc gtttattcac gccgatcagg
    19561 ttccccgcgc tcagcatatt ttgcagggtg aacaaggctt tcgtctgaca ccgctggcgt
    19621 taaaggattt tcaccgccag ccggtgtatg gcctttactg tcgcgcccat cgccaattga
    19681 tgaattacga aaagcgtctg cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc
    19741 cgccagaacg ctatctgatg gagcggttta tcacctcacc ggtgtgggtc gagggtgata
    19801 tgcacaatgg cactatcgtt aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc
    19861 tcaagtgggt ttctatagat attgaaacca cccgccacgg tgagctgtac tgcatcggcc
    19921 tggaaggctg cgggcagcgc atcgtttata tgctggggcc ggagaatggc gacgcctcct
    19981 cgcttgattt cgaactggaa tacgtcgcca gccgcccgct gctgctggaa aaactcaacg
    20041 cctggtttgc aaaccacgat cccgatgtga tcatcggctg gaacgtggtg cagttcgatc
    20101 tgcgaatgct gcaaaaacat gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata
    20161 atagcgagct ggagtggcgc gagcacggct ttaaaaacgg cgtctttttt gcccaggcta
    20221 aaggtcggct aattatcgac ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt
    20281 cattctcgct ggaaactgtc gctcaggagc tattaggcga aggaaaatct atcgataacc
    20341 cgtgggatcg aatggacgaa attgaccgcc gtttcgccga agataaacct gcgctggcaa
    20401 cttataacct gaaagattgc gagctggtga cgcagatctt ccacaaaact gaaatcatgc
    20461 catttttact cgaacgagca acggtgaacg gcctgccggt ggatcggcac ggcggttcgg
    20521 tggcagcgtt tggtcatctc tattttccgc gaatgcatcg cgctggttat gtcgcgccta
    20581 atctcggcga agtgccgccg cacgccagcc ctggcggcta cgtgatggat tcacggccag
    20641 ggctttatga ttcagtgctg gtgctggact ataaaagcct gtacccgtcg atcatccgca
    20701 cctttctgat tgatcccgtc gggctggttg aaggcatggc gcaacctgat ccagagcaca
    20761 gtaccgaagg ttttctcgat gcctggttct cacgggagaa acattgcctg ccggagattg
    20821 tgactaacat ctggcacggg cgcgataaag ccaaacgtca gggtaataag ccgctttcgc
    20881 aggcgctgaa gatcattatg aatgcctttt atggcgtgct cggcaccacc gcctgccgct
    20941 tcttcgatcc gcgcctggca tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaga
    21001 ccaaagcatt gattgaagcg cagggctacg acattatcta cggagatacc gactcgactt
    21061 ttgtctggct aaaaggtgca cattcggaag aagaggcggc gaaaatcggt cgtgcactgg
    21121 tgcagcacgt taacgcctgg tgggcggaaa cgctgcaaaa acaacggctg accagcgcat
    21181 tagaactgga gtatgaaact catttctgcc gttttcttat gccaaccatt cgcggagctg
    21241 ataccggcag taaaaagcgc tacgccgggc tgattcagga gggcgacaag cagcggatgg
    21301 tgtttaaagg gctggaaacc gtgcgcaccg actggacgcc gctggcccag cagtttcagc
    21361 aggagctgta cctgcgtatc ttccgcaacg agccatatca ggagtacgtc cgccaaacca
    21421 tcgacaaact gatggcgggt gaactggatg cgcggctggt ttaccgtaaa cgccttcgcc
    21481 gtccgctgag cgaatatcag cgtaatgttc cgcctcatgt acgcgccgct cgccttgccg
    21541 atgaagaaaa tcaaaagcgc ggtcgcccct tgcaatatca gaatcgcggc accattaagt
    21601 acgtatggac caccaacggc ccggagccgc tggactacca acgttcacca ctggattacg
    21661 aacactatct gacccgccag ctacaacccg tggcggaggg aatactccct tttattgagg
    21721 ataattttgc tacacttatg accgggcaac ttgggctatt ttgagcaaaa aaaagagttc
    21781 gccagatacc attttgatgc gtgacgaatg ctttgccatc cagtaccata gcgccctttc
    21841 cattcctgga cctgaataac accactacct cataagcacg gtagcgggtg gttattgcct
    21901 gcaattaaag atatagagcc gaacacatat gccttttaca cttggtcaac gctggatcag
    21961 cgatacagaa agcgaattgg gacttggaac cgttgtcgcg gtggatgcgc gaactgtcac
    22021 tttacttttc ccatctactg gtgaaaaccg tctgtacgca cgcagtgatt cccccgtgac
    22081 ccgcgtgatg ttcaaccctg gtgataccat taccagccat gacggctggc agatgcaagt
    22141 cgaagaagta aaagaagaaa atggcttgct gacctatatc ggtactcgcc tggatactga
    22201 agagtccggc gtagccctgc gtgaagtttt ccttgatagc aaactggtgt tcagcaaacc
    22261 gcaggaccgt ctgtttgccg ggcagattga ccgtatggac cgctttgcgc tgcgttatcg
    22321 cgcgcgtaag tattccagcg aacagttccg tatgccgtac agcggcctgc gcggtcagcg
    22381 taccagcctg atcccgcatc agctcaacat cgctcatgat gttggtcgcc gccacgcgcc
    22441 gcgcgtcctg ctggctgacg aagtgggttt agggaaaacc attgaagccg ggatgatcct
    22501 gcatcagcaa ctgctctctg gcgctgctga acgtgtgctg attatcgtcc cggaaacctt
    22561 acagcatcag tggctggtag aaatgctgcg ccgtttcaac ctgcgctttg ctctgtttga
    22621 tgatgagcgt tatgccgaag ctcagcacga tgcttacaac ccgtttgaca ccgaacagct
    22681 ggtgatttgc tcgctggatt ttgcccgtcg tagcaaacag cgcttggaac atctctgtga
    22741 agccgaatgg gacctgctgg tggtcgatga agcgcatcac ctggtgtgga gcgaagatgc
    22801 gccaagccgt gaatatcagg ccattgaaca actggcagag cacgtgccgg gcgttctgct
    22861 gctgaccgcg accccggaac agctggggat ggaaagccac ttcgcccgtc tgcgtctgct
    22921 ggacccgaac cgtttccacg attttgcgca gttcgttgaa gagcagaaaa attatcgtcc
    22981 ggttgcggac gccgttgcca tgctgctggc aggtaacaaa ctgagcaatg acgaactgaa
    23041 catgctcggc gagatgatcg gcgagcagga tatcgagccg ctgttgcagg cagcaaacag
    23101 cgacagcgaa gatgcccaga gcgcccgtca ggagctggtt tcgatgctga tggatcgcca
    23161 cggcaccagc cgcgtgctgt tccgtaacac gcgtaacggt gtgaaaggat tcccgaaacg
    23221 cgagctgcac accattaagc tgccgctacc gacgcagtat cagacggcta ttaaagtctc
    23281 cggcattatg ggcgcacgta aaagtgcgga agatcgtgct cgcgatatgc tctacccgga
    23341 gcgtatttat caggaatttg aaggtgataa cgccacctgg tggaacttcg atccgcgcgt
    23401 tgagtggctg atgggctacc tgaccagcca tcgctctcag aaagtgctgg tgatctgcgc
    23461 caaagctgcc actgcgctgc aactggagca ggtactgcgc gaacgtgaag gtattcgcgc
    23521 tgcggtgttc cacgaaggta tgtcgattat cgaacgtgac cgcgctgccg cctggtttgc
    23581 cgaagaagac accggcgcac aggtactgct gtgctcagaa atcggttctg aaggacgtaa
    23641 ctttcagttc gccagtcaca tggtgatgtt tgacctgcca ttcaacccgg atctactgga
    23701 gcagcgtatt ggtcgtctgg atcgtatcgg ccaggcgcac gatattcaga tccatgtgcc
    23761 ttatctggag aaaaccgctc agtcggtgct ggtgcgctgg tatcacgaag gtctggatgc
    23821 atttgagcac acctgcccga ccggacgcac tatttacgat agcgtataca acgatctgat
    23881 taactatctg gcttcaccgg atcaaaccga aggctttgac gatctgatca aaagctgccg
    23941 cgagcaacat gaagcgctga aagcacagct ggaacagggt cgtgaccgcc tgctggaaat
    24001 ccactccaac ggtggcgaaa aagcccaggc actggcagaa agcattgaag agcaggatga
    24061 cgataccaac ctgatcgcct tcgccatgaa cctgttcgat attatcggta tcaatcagga
    24121 cgatcgcggc gacaacatga tcgtgctgac gccgtccgat catatgctgg tgccggactt
    24181 ccctggcctg tcggaagatg gcatcaccat cacctttgat cgtgaagtgg cgctggcgcg
    24241 tgaagatgca cagtttatta cctgggagca tccgctgatc cgcaacggtc tggatctgat
    24301 cctttctggc gataccggta gcagcacgat ttcactgtta aaaaacaaag cgttgccggt
    24361 aggtacgctg ttggtggaac tgatttatgt ggttgaagcc caggctccga agcagttgca
    24421 gctcaaccgc ttcctgccac cgacgccggt acgtatgctg ctggataaaa acggcaacaa
    24481 cctggcggcg caggtagagt ttgaaacctt taaccgccag cttaacgcgg ttaaccgtca
    24541 caccggcagc aaactggtta acgccgtgca gcaggatgtt cacgctatcc ttcaactggg
    24601 tgaagcgcag atcgagaaat ctgcccgtgc attgattgat gcagcgcgta acgaagccga
    24661 cgaaaaactg tctgccgagc tgtctcgtct ggaagctctg cgtgcagtga acccgaacat
    24721 tcgtgacgac gaactgaccg ccattgagag caaccgtcag caggtaatgg aaagcctgga
    24781 tcaggcaggt tggcgtctgg atgccctgcg tttgatcgtt gtaacgcatc agtaacggag
    24841 ccgaaaatgg ggatggaaaa ctacaatcca ccgcaggaac cctggttggt tatcctgtat
    24901 caggatgacc atattatggt ggtcaacaag ccgagcggtt tgttgtcagt gccgggtcgt
    24961 ctggaagagc acaaagacag cgtgatgacg cgcattcagc gtgattatcc gcaggcagaa
    25021 tcggtgcatc gtctggatat ggctaccagc ggcgtgattg tagtggcgct gaccaaagcc
    25081 gcggagcggg agttaaaacg ccagttccgc gagcgcgagc cgaaaaagca gtatgtggcc
    25141 cgcgtctggg ggcatccatc ccccgcagaa ggtctggtgg atctgccgct gatttgcgac
    25201 tggccaaacc gcccgaaaca gaaagtctgt tacgaaacgg gtaaacctgc gcagacggaa
    25261 tatgaagtgg tggagtatgc ggcggataac acggcaagag tggtgttaaa accgattacc
    25321 gggcgttcgc atcaattgcg tgtgcatatg ctggcgctgg ggcatccgat tctcggcgat
    25381 cgtttttatg catcaccaga agcgagagcg atggcaccgc gtttgttgct acatgcagag
    25441 atgttgacga ttacccatcc ggcgtatggc aatagtatga cgtttaaagc gccagcggat
    25501 ttttaatcgt cacgctggcg cagttcggta ggcggataag gcgttcacgc cgcatccggc
    25561 aattgtgcgc cgattgcctg atgcgacgcg ggccgcgtct tatcaggcct acagtttaaa
    25621 acttatttaa accctttctg ctgctttatc agctcatatg cctgctgaat ttcctgcgct
    25681 ttctgcttcg ccatctccat catctcaggc ggcaaacctt tcgccaccag cttatcggga
    25741 tggtgttcac tcatcagctt acggtaggca cgtttgatgg tggtcgcatc atccgtcggc
    25801 ttcacgccca gcacattaca ggcatcttcc agcgtcgggc cacgctgcgc ttgctgccag
    25861 ttaccaccgc cagattgctg ctgataaccg ccgccaaact gtgcaccgcc ctgcatcatg
    25921 cgcaaaaact ggtcaaactg ggcgcgggag atccctaatt cttcagcaat gacatacagc
    25981 accgcccgtt cattcgggtg cagtgaacca tcagcaaacg ccgcctgaat ctggatctcc
    26041 agaaacatac gaattaagtc aaaacgacca aagcagacac tgcgaaactg gcgcatcttt
    26101 tcgcgcagcg ggtaattatc tgattttccc acccggaacg cgttttgcgc cgcagtacgg
    26161 gaagcgccat gaaggttcat tcggtccatc aactggctgg cgatatgaat atcagcctcc
    26221 gtgacgcgac ctttggattt ggttaaatgc cccatcactt caaaagtggt ggcaaaaaac
    26281 agcgcctgac gctcacgctg gttggcgaac cacgccattt tacggctacg ggctttatca
    26341 aacatatggc caattaacag gcctaacact acgccccaaa agccgccgcc catcagtaag
    26401 gccacggcca cgccaatgat ttttccccaa tactgcatat attccccaaa tcgacacacg
    26461 gatatcaggg ctatctccca caatataaag gtgcttttac cgttttccgg ctgcggtcaa
    26521 ttgtgggaca tcgcctataa tttgcattat catacctgtc attcaatgcc gtgcctaaca
    26581 ccactgacgc tattcggaca ggattaacac tagcgtagag atgacgagta cgttagtctc
    26641 tgaccgtttg tcacgcgcaa cgttaccgat gatggaacaa taaaatcaac gtatgaaaaa
    26701 acgtatcccc actctcctgg ccaccatgat tgccaccgcc ctttatagtc aacagggact
    26761 ggcagccgac ctcgcctcac agtgcatgtt gggcgtgcca agctatgacc gtcctctggt
    26821 acagggcgat accaatgact tacccgtgac tatcaatgct gaccacgcga aaggggacta
    26881 cccggatgac gccgtgttta ctggcagcgt ggatatcatg cagggtaaca gccgtctgca
    26941 ggccgacgaa gtgcagctcc atcaaaaaga ggcaccagga caaccggagc cggtacgtac
    27001 cgttgatgcg ctcggtaatg tccattacga cgataaccag gtgatcctca aagggccgaa
    27061 aggctgggcg aatctgaaca ccaaagatac caacgtctgg gaaggtgatt accagatggt
    27121 gggtcgccag ggtcgcggta aagcggacct gatgaaacaa cgtggcgaaa accgctatac
    27181 cattctggat aacggtagct ttacctcctg tctgccgggt tctgacacct ggagcgtggt
    27241 aggtagcgaa attattcatg accgcgaaga acaagttgct gagatctgga acgcccgctt
    27301 taaggtgggt ccggtaccga tcttttatag cccctatttg cagttgccgg tgggtgacaa
    27361 acgtcgctct ggtttcttga tcccgaacgc caagtacacc accaccaact actttgagtt
    27421 ctacctgcca tattactgga acatcgcgcc aaatatggat gccaccatca cgccgcatta
    27481 tatgcatcgt cgtggcaaca tcatgtggga gaacgaattc cgctacctct cccaggcggg
    27541 cgctggcttg atggaactgg actatctgcc ttcagataaa gtctatgaag atgaacaccc
    27601 gaacgatgac agttcacgtc gttggttatt ctactggaac cactccgggg tcatggatca
    27661 ggtgtggcgt ttcaacgtcg actacaccaa ggtcagcgat cctagctact tcaatgattt
    27721 cgataacaag tacggttcca gtactgacgg ctacgcaacg caaaaattca gcgttggcta
    27781 tgcggtgcaa aacttcaatg ccaccgtttc aaccaagcag ttccaggttt tcagcgaaca
    27841 gaacaccagt agctactcgg cagagccgca gttagacgtt aattactacc agaatgatgt
    27901 tggtccgttt gatacgcgta tttacggcca ggcagtgcac tttgttaaca ccagagacga
    27961 catgcctgaa gcaacccgtg ttcacctgga accgaccatc aatttgccgc tctctaataa
    28021 ctggggcagc atcaataccg aagcgaagtt tctggcaacc cattatcagc aaaccaatct
    28081 tgactggtat aactccagaa acacgaccaa gctggacgaa tccgttaacc gcgtaatgcc
    28141 gcaattcaaa gttgacggca aaatggtctt tgaacgcgat atggaaatgc tggctccggg
    28201 ttatacccaa acgctggaac cgcgcgcgca gtatttgtac gtgccgtatc gcgatcagag
    28261 cgacatctat aactacgact cgtctctgct gcaatctgac tactctggcc tgttccggga
    28321 ccggacttac ggcggtcttg accgtattgc ctccgctaac caagtgacga ccggtgtcac
    28381 atctcgcata tatgatgatg ctgccgttga acgttttaat atttccgttg gtcaaatcta
    28441 ctatttcacg gagtctcgca ctggcgatga caacataaca tgggagaatg acgacaaaac
    28501 gggctcactg gtgtgggcag gcgacaccta ctggcgtatc tccgagcgtt ggggattgcg
    28561 tggcgggatt cagtacgata cacgtctgga taacgtagcg accagtaact ccagcattga
    28621 ataccgtcgg gatgaagacc gtctggtaca gctgaattac cattacgcca gcccggaata
    28681 tattcaggct acgctgccta agtactattc cactgctgag caatataaga atggtatttc
    28741 gcaggtaggt gctgtcgcca gcaggccaat tgccgatcgt tggtccattg ttggggccta
    28801 ctactacgac accaatgcga acaagcaagc cgactctatg ttaggtgtgc aatacagctc
    28861 ctgctgctat gcaattcgcg tcggttacga gcggaagctg aacggttggg ataacgataa
    28921 acaacatgcg gtatatgaca acgcaatcgg ctttaacatc gaacttcgcg gcctgagctc
    28981 caactacggt ctgggtacgc aagagatgct gcgttcgaac attctgccgt atcaaaacac
    29041 tttgtgattt gttgatttac cacgtaatcc gcagtgcggt taattgaaat ggaaaaagta
    29101 tgaagaactg gaaaacgctg cttctcggta tcgccatgat cgcgaatacc agtttcgctg
    29161 ccccccaggt agtcgataaa gtcgcagccg tcgtcaataa cggcgtcgtg ctggaaagcg
    29221 acgttgatgg attaatgcag tcggtaaaac tgaacgctgc gcaggcaagg cagcagcttc
    29281 ctgatgacgc gacgctgcgc caccaaatca tggaacgttt gatcatggat caaatcatcc
    29341 tgcagatggg gcagaaaatg ggagtgaaaa tctccgatga gcagctggat caggcgattg
    29401 ctaacatcgc gaaacagaac aacatgacgc tggatcagat gcgcagccgt ctggcttacg
    29461 atggtctgaa ctacaacact tatcgtaacc agatccgcaa agagatgatt atctctgaag
    29521 tgcgtaacaa cgaggtgcgt cgtcgcatca ccatcctgcc acaggaagtc gaatccctgg
    29581 cgcagcaggt gggtaaccaa aacgacgcca gcactgagct gaacctgagc cacatcctga
    29641 tcccgctgcc ggaaaacccg acctctgatc aggtgaacga agcggaaagc caggcgcgcg
    29701 ccattgtcga tcaggcgcgt aacggcgctg atttcggtaa gctggcgatt gctcattctg
    29761 ccgaccagca ggcgctgaac ggcggccaga tggggtgggg ccgtattcag gagttgccgg
    29821 ggatcttcgc ccaggcatta agcaccgcga agaaaggcga cattgttggc ccgattcgtt
    29881 ccggcgttgg cttccatatt ctgaaagtta acgacctgcg cggcgaaagc aaaaatatct
    29941 cggtgaccga agttcatgct cgccatattc tgctgaaacc gtcgccgatc atgactgacg
    30001 aacaggcccg cgtgaaactg gagcaaattg ctgctgatat caagagtggt aaaacgactt
    30061 ttgctgccgc agcgaaagag ttctcgcagg atccaggttc tgctaaccag ggcggtgatc
    30121 tcggctgggc tacaccagat attttcgatc cagccttccg tgacgccctg acccgcctga
    30181 acaaaggtca aatgagtgca ccggttcact cttcattcgg ctggcattta atcgaactgc
    30241 tggatacccg taatgtcgat aaaaccgacg ctgcgcagaa agatcgtgca taccgcatgc
    30301 tgatgaaccg taagttctcg gaagaagcag caagctggat gcaggaacaa cgtgccagcg
    30361 cctacgttaa aatcctgagc aactaatggt taaaacccaa cgtgttgtga tcactcccgg
    30421 cgagcccgcc gggattggcc cggacttagt tgtccagctt gcacagcgtg agtggccggt
    30481 cgaactggtt gtttgtgccg atgccactct ccttaccgac cgggcagcga tgctcggttt
    30541 gccgctcacc ctccgccctt attcccccaa ctcccctgca caaccgcaaa ctacgggcac
    30601 attaacgcta cttcctgtcg cgctacgtga atctgtcact gcggggcagt tagcgattga
    30661 aaatgggcat tacgtggtgg agacgctggc gcgagcgtgt gatggctgtc tgaacggtga
    30721 atttgctgcg ctgatcacag gccccgtgca taaaggcgtc attaacgacg caagcattcc
    30781 gtttaccggt cataccgagt ttttcgaaga gcgttcgcag gcgaaaaaag tggtgatgat
    30841 gctggcgacc gaagaacttc gcgtggcgct ggcaacgacg catttaccgc tgcgcgatat
    30901 cgcagatgct atcacccctg cacttttgca cgaagtgatt gctattttgc atcacgattt
    30961 gcgtacaaaa tttggtattg ccgaaccgcg cattctggtc tgcgggctga atccgcacgc
    31021 gggcgaaggc ggtcatatgg gtacggaaga gatagacacc attattccgg tgctcgacga
    31081 gctgcgggcg caggggatga aactcaacgg gccgctgcct gccgataccc tgtttcagcc
    31141 gaaatatctc gataacgccg acgccgtgct ggcgatgtac cacgatcagg gtcttcccgt
    31201 gctaaaatac cagggcttcg ggcgcggtgt gaacattacg ctgggcctgc cctttattcg
    31261 cacatcagtg gaccacggca ccgcgcttga actggcggga cgtggcgaag ccgatgtcgg
    31321 cagttttatt acggcgctta atctcgccat caaaatgatt gttaacaccc aatgaataat
    31381 cgagtccacc agggccactt agcccgtaaa cgcttcgggc aaaactttct caacgatcag
    31441 ttcgtgatcg acagcattgt ttctgccatt aacccgcaaa agggccaggc aatggtcgaa
    31501 atcggccccg gtctggcggc attgaccgag ccggtcggcg aacgtctgga ccagctgacg
    31561 gtcatcgaac ttgaccgcga tctggcggca cgtctgcaaa cgcatccatt cttaggcccg
    31621 aaactgacga tttatcagca ggatgcgatg acctttaact ttggcgaact ggccgagaaa
    31681 atgggtcagc cgctgcgtgt tttcggcaac ctgccttata acatctccac gccgttgatg
    31741 ttccatctgt ttagctatac tgatgccatt gccgacatgc actttatgtt gcaaaaagag
    31801 gtggtgaatc gtctggttgc aggaccgaac agcaaagcgt atggtcgatt aagcgtcatg
    31861 gcgcaatact attgcaacgt gatcccggta ctggaagttc cgccgtcagc ctttacacca
    31921 ccacccaaag tggattccgc cgtcgtgcgc ctggtccctc atgcaacgat gcctcacccg
    31981 gttaaagata ttcgcgtgtt gagccgcatc accaccgaag cctttaacca gcgtcgtaaa
    32041 accattcgta acagcctcgg caacctgttt agcgtcgagg tgttaaccgg aatggggatc
    32101 gacccggcga tgcgagcgga aaatatctct gtcgcgcaat attgccagat ggcgaactat
    32161 ctggcggaga acgcgccttt gcaggagagt taacgatgat caattcgccc cgagtgtgta
    32221 ttcaggttca aagcgtctac attgaggcac aatcttcacc tgataatgaa cgttacgttt
    32281 ttgcttatac cgtaaccata cgcaatctgg ggcgagcgcc agtgcagttg ttggggcgtt
    32341 actggctgat caccaatggc aatggccgtg aaaccgaagt ccagggcgaa ggagtggttg
    32401 gcgtccagcc acttatcgcg cctggcgaag agtaccagta caccagcggt gcaatcattg
    32461 aaaccccgct gggcaccatg cagggtcact acgaaatgat cgatgaaaat ggcgtccctt
    32521 tcagcatcga cattcccgta ttccgactcg ccgttcccac actcattcat taaaagaata
    32581 tggcgacata ccttattggc gacgttcatg gttgttacga tgaactgatc gcattgctgc
    32641 ataaagtaga atttaccccc gggaaagata ccctctggct gacgggcgat ctggtcgcgc
    32701 gcggcccggg ttcgctggat gttctgcgct atgtgaaatc cttaggcgac agcgtacgtc
    32761 tggtgctggg caatcacgat ctgcatctgc tggcggtatt tgccgggatc agccgcaata
    32821 aaccgaaaga tcgcctgaca ccgctgctgg aagcgccgga tgccgacgag ctgcttaact
    32881 ggctgcgtcg ccagcctctg ctgcaaatcg acgaagagaa aaagctggtg atggcccacg
    32941 cagggatcac gccgcagtgg gatctgcaga ccgccaaaga gtgcgcgcgc gatgtagaag
    33001 cggtgctgtc gagtgactcc tatcccttct ttcttgacgc tatgtacggc gatatgccaa
    33061 ataactggtc accggaattg cgggggctgg gaagactgcg ttttatcacc aacgccttta
    33121 cccgtatgcg tttttgcttc ccgaacggtc aactggatat gtacagcaaa gaatcgccgg
    33181 aagaagcccc tgccccactg aaaccgtggt ttgcgattcc tggccctgtc gctgaagaat
    33241 acagcatcgc ctttggtcac tgggcatcgc tggaaggcaa aggtacgccg gaaggtattt
    33301 acgcgctgga taccggctgc tgctggggtg ggtcattaac ctgcctgcgc tgggaagata
    33361 aacagtattt tgtccagccg tcgaaccggc ataaggattt gggcgaagcg gcggcgtctt
    33421 aaacacagcc tgatacagga aggccggata agacgcgacc ggcgtcgcat ccggcactag
    33481 ccgtaaattc tatacaaact taccgccgct ccagaatctc aaagcaatag ctgtgagagt
    33541 tctgcgcatc ggcgtcgtgg aattcgctga ataccgattc ccagtcatcc ggctcgtaat
    33601 ccgggaaatg ggtgtcgcct tccacttctg cgtcgatatg cgtcagatac agtttttgcg
    33661 cttttggcag gaactgctca taaacgcgac cgccgccaat caccatgatt tctggtacgt
    33721 caccacacgc cgcaatggct tcatccaccg atttcaccca cgttacgcga tcgtccgtac
    33781 ccggttgact gctgaggata atatttttgc gtcctggcaa cggacgaccg attgattccc
    33841 aagtatggcg gcccataatc acgggtttat ttaaggtgtt gcgtttaaac caggcgagat
    33901 cggcaggcag gttccacggc atggcgtttt ccatgccgat aacgcgatct accgctaacg
    33961 ccgcaatcag actgatcatt gagatttccc gataaaaaaa ttgtcgccac tatacgtaaa
    34021 gcgtaaaccg tcgtcgactg gtgcgaggat gatgttgagg aaaattttat attctgctgg
    34081 cgagtccacg ctctccccct ggactcgccg cattacaatg aaacaaaaac aaacagttag
    34141 ctgtaaagtg tgatttacgt cactctttat taagatgagg gtttcgtttc cggttcatcc
    34201 gccatgttgc cggtatgttt accttcttct gttccctgcc agccatgtcg ttgaattaat
    34261 gacagatgtt cgcggtcctc ggtgatgatc tcacttaaca tcgcgctggt gcgtttatag
    34321 accgccgcgc gggctttggt gtcattctca accattgcca tctcttccac catctgaata
    34381 ttaaagcggc ggaacacatc ggcacgttct cgcgcttcat acggccccag acctaaactt
    34441 tccagcgcca gacgcccggt tttcagcgca ccttcaaagg tttcacgctc cggcttttca
    34501 acgcctgcct gacgcaaacg aatgtagtga tcgacatcgc gggcgcgggc aataatctgc
    34561 aaatgcggga aatgttcttt caccatctcc gtcagttgca ggttggtttg cggatcgtca
    34621 atagcgttaa tcagcacttc cgctttcgcc gctccggcag attccagtaa atccatccgc
    34681 gtggcatcgc cataaaacac tttcatacca aatttacgca gcgtttcgat atggtccgga
    34741 tcgtgatcga gcaccaccat tttcaccccg ctggagagca gtaaacgtcc ggtaatctgt
    34801 ccaaaacgac cgaatccggc gataatcacg cgcggctgtt cttcatcgat ctcatcggct
    34861 tcacgcgcct cctccgtaga agattgctca aggcgattga ggatcaccag cagaatcggc
    34921 gttgctgcca tcgacagcgc caccgccagg gtcagcgatt tcgcccactc cggctccagc
    34981 acattcgcca tctgcgccgc accaaatacc acaaaggcaa actcactgcc ctgccctaac
    35041 aacaccgcaa accagcgacg ctgtttattt ggcacctgca acggtcgggc aatcagccac
    35101 agcatggcga ttttgatgat gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt
    35161 tcaatcagcg tgccaaagtc tatcgacatg ccaacaccga tgaaaaacag ccccaacagc
    35221 aaacctttaa acggttcgat atcgctttcc aacgcatgac ggtattccga gcttgccagc
    35281 agtacgcccg ccagaaacgc gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa
    35341 ccaaagccaa acacaaggaa taacgccacg gcactaaaca cttcccgcaa gccagagcgg
    35401 gcaacaaaac gcagcgccgg acgcgtaaca tagcgcccca gcaataccac cagtaccagc
    35461 gcacccgcca cttttaacgc cgagagagca aatgcgccca tcgtcgtcga ggcactgctc
    35521 gccgccagta gcgggatcat cgccaccagc gggattgccg cgatatcctg gaacagcagc
    35581 actgcaaagg cactgcggcc catttgcgtc accatcagat tgcgttcatt catcgcctgc
    35641 atggcaatcg ccgttgatga gagcgccagc gtcataccga tcaattccgc gacctgccag
    35701 cgcagcccaa gtaacataca gaacagcccc agcaggccac cgcaaatcac catctgcaat
    35761 gcgccaccgc cgaacactgc cgcacgcagc ttccacagcc tttgtggatc gagttcgagg
    35821 ccgataataa acagcatcag caccacccca atctcggcaa agtgcagaat agattcggca
    35881 tcggtcacca gtcgcagccc ccacgggcca ataatgcagc cggcgatcag gtagccaagt
    35941 accgatccca gaccaagacg taccgcaatg ggtacaatca gcgccgccga tccgaggtag
    36001 atcagcgcct gaaccagcgt atgactatcc atgatgggcc tcctgccatt ccagcagacg
    36061 ttgcttatag tggcgcgcct gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg
    36121 catggcaaac ggtggcagcc agttcagccc gcagtagatt gccgtcgcct gtagcggctg
    36181 cgacagcaca tcaaagcccg gatgcgcacc aatttcaaaa tggctttccc cgccgccggt
    36241 cgtcaccgcc cacagcaaat gtttgccatg cagcgccgtg ccgccgtgac cgtaagccca
    36301 gccgtgcgac aaaactttat cgatccaaag tttaagaagc ggaggaatgc tgtaccactg
    36361 catcgggtgc tgccagacga tcagatcggc gcgagagagc gcctcctgct cggcggcaat
    36421 atcgatattg aagtcaggat agagttgata aagagagcga atttcgacgc cttccagcgt
    36481 ccttgcctgt tcaagcatcc gtttattcgc atgggaatga tgcggatacg gatgcgcata
    36541 aatgatgagg atcatagtta ttagcccgcg tgtttacgcc tttgttttcc cacaaagtgt
    36601 agtcatatat tcacgcggct tatagtcaat aatattgatt cagaaaagag agaaatctga
    36661 acaaataatc ccattctctg cccgcggatc gtcatattgc tggtctgcgc cagtgacatt
    36721 cctcgagtct ccggggcaaa cgctacggaa atcaacaagc caaacagcga gatacccgcc
    36781 cccattagca tcgtgttact gataccgtaa ttattgataa atatcggcag tgcccaggtc
    36841 gaaacaatgg tgccaatacg acttaaggac ataatcacgc ccacggcaga ggcgcggata
    36901 tccgtcggga agagttcatt aggatagagc cactgcaaat tacccggccc gccagagaaa
    36961 aaggcataca ccgcaaaggc catcactacc agccagatcc ccatatccgg gattagcccc
    37021 aaaaccgcca acgccagcgt catcatggca aagctgccaa tcaacaatgg acgccgtccg
    37081 gcagtgttta gccacagcat cggcggaata cagccgagca taaagaacag gctaatcacc
    37141 acattcccta gtgccgcgtt tttgccaacc cccaatccca acgaaccaac gatttgcggg
    37201 ccaaaggtgt aaatggcgaa cattgggatc acctggcagg tccagatggc ggcaacaaac
    37261 agaacaaaag gaaaatggcg gcgattaaac agatcgcgaa aacgggtttg ctgcggctgc
    37321 tcttcatcga aagccaccgg ttcgccaaac agttttatca tcatctcttc gcactctttt
    37381 actcgccctt tgcgtaataa ccagcgggga gattctggca gttcgaatcg accaatcaaa
    37441 atcaacaaac aagggatcgc cgcgctaccc agcatccagc gccaaccacc ttccacatca
    37501 taaagccagt agccgaccag atcggcgcag gtcgcgccga cataccacat cgcggcaata
    37561 aagctgatgg aaaacgcccg ctgacgggta ctggagaact cggtgatcat cgaagtggcg
    37621 atgggataat ctgcaccgat gacaatgccg atgagtaccc gcatcaccaa cagttcgacg
    37681 ggggatgaaa cacacatcgt cgccaccgat atcacgccga tggcgatgat atcaatgagg
    37741 aacattttgc gccgtccgac tttatcggag atatagccaa acagcgatgt gccaacgaac
    37801 agcccggcga gcgttcccgc gcccagcaag ccaatccagt cagcgtccag tttcagcgcc
    37861 ggagtcagtt gctccagcgc cacgccaatc attaccagta cataaccatc cagaaacgga
    37921 ccaccgcttc cccacagcaa aatgcggcgg tgaatagagg agaatttgag atcgtcaaag
    37981 tttctggacg gttgcatggg ttattcctgt ttttttgcaa tgagagtcag tacggcgtta
    38041 cgtatcaaac caacatcagc cgtaacggaa ctccacacca aaggtgccgc gcgggtattc
    38101 ccactgttcc agcgccgacc ccagccccag aatgcgacag gtgccgcact ccagacatcc
    38161 ggcgtaatcg aagcgcacac tgccgtcatc ctgcttcttg tacagacctg cggggcacgc
    38221 tttcaccagc agctccagca cctgtttatc agcatcggcc ttcacaacaa tgtgcggatg
    38281 ctcttcatcg acattgaatt tattgacgcc cagtttgacg tccacattga cgggagaagt
    38341 cataaaacgg tcactccttt catgccatcc ttgatcagat tgatgaagcc cactttcttg
    38401 ccgtggcgga ggattttctt gcgcatcagt tccggcgcac tgccgtcaat ggtgaacagg
    38461 tcacgcgcca cgcccaccgc cagttccggg ttgccgctaa acatgcgtgg gttatcaagg
    38521 aacgccggta gtttctggta catgcgcata tcgcgcagcg ggccactctc cagatgctga
    38581 cgatattccg cgagtttttg cttactgaaa tcgtcgcttt tcatcgctga aagcacggtt
    38641 tttgctgcag cttccccggc ggcaatcgcc agatccatac cgcgaatggt aaaaccgagg
    38701 ttcatacaca ttccggcggc atcaccggca atcaatacgc cgtcaccgac cagctccggc
    38761 agcatgttga tgcctgcttc cggcactacg tgagcggaat attccaccag cttgccgccc
    38821 gcgatcagcg gtgcaacggc cggatgctgt ttgaaatctt ccagcatttg cggcaccgat
    38881 tttttcgcgt catgcagatg atgcagacca caaaccagcc ccagcgacag ggtattttca
    38941 ttggtataaa ggaagccgcc gcccatcagg ccatcggtgg gtgatcccgc aaacagacaa
    39001 gccgcgccct gattaccctg caactgaaaa cggtcttcga ttaccgactt cggtaactcg
    39061 atcagttcct tcacgccaac cgccacatcc gtcggtttaa cgcgttttgc catccccaac
    39121 ttttcggcaa ggatggagtt caccccatca gcaaggagca ccgttttcgc ttcaatcaca
    39181 tcaccatcgg cttctacacc gacgactttg ccatcgcgct gtacgaggtt atcgacgcgg
    39241 atcccggtaa ttaactgcgc gcccgcttct tcggcctgct ccatcaacca ggcatcaaat
    39301 ttactgcgca aaacggagta agaacgctgg gatggcgagg tttcgtcacc gttgcagtag
    39361 tccatagtca tcgctgactt ttccgtcata aacgcgagtt tttcatgggt gatcaggcgt
    39421 tctacggggg cggaatcggc gaaacctgga ataatgtgtt ccagactgtg ggcatagaga
    39481 cgcccgccag taacgttctt ggcacctgcg gaattgccac gctcgataac taacacttgc
    39541 gccccttcgc gggcgagcac cagtgcggca accgaaccgg caagccctgc accgacgatg
    39601 atggcgtcaa agatatcttc ggacataaga gctccctgtc agcggcagag ccgtaatgca
    39661 gccctgccag agtggatcaa cgcgctaaag ctgcggtcag tgccggaagg atcttcacgg
    39721 catcgccaac aatgccgtaa tccgcgtact ggaagatcgg cgcattttta tctttattga
    39781 tggcgaaaat ggtttgcgac gcgttagcgc caaccatgtg ctggatctac ccggagatcc
    39841 ccaccgccag gtacagttca ggtttcagca tcaggttgga gataccgaca tagcgttcgt
    39901 gctccatcca tttttcgttt tctgccaccg gacgagaaca ggccaactcc gcacctatcg
    39961 ccttgcaaag ctgttctgcc agcgcaatgt tctctttgct gccaatgccg cgaccgacgc
    40021 tgaccaccag acgggctttg tcgagatcga cgctgttgct ctggcgcgcc tgggttgccg
    40081 tgcgggtaat cgccacagcc ggagcctgcc actccacggt gtgcgtttcg ccagtgcgtg
    40141 acgcgtctgg ctgagccgca tcgaacgtgc cgctgctgat ggtcagtacc gcatacggcg
    40201 tggcaatgcg ttcttcgcca atcgccagac cgccgtaaac catgtgtttc actgttgctt
    40261 taccgtcctg tacgctgacg gtgctggcat cgttagacac cgccgcttta aggcgataac
    40321 ccagttttgc cgccagtaat ttgccgcgac gggtgtttgg caacagcacc agaccgtcag
    40381 cgccgtgctg gcgaatagtg tcagccatga caccggcgta atcttcgatc atccgatcgt
    40441 ccggtttgcc gtttaatttc cagacatgat tagcgccgag ctggattgcc tgtgcgccgt
    40501 cggcatcatt gaggacaaag gcattgattt gattagctaa agcctgcgca ccgttcatca
    40561 gttccggcag acgagagggg gtatcgctga atacccagac ttgagaaaac gtgttcatag
    40621 catcccctgt aattaaatga ctttgcgaag attttcagca aatgcggcga tctgttcttc
    40681 gccgtcgcct tcaatcacga tgcgctgacg ctcgcgctgt ttcggcgcgg caacctgttg
    40741 ttcagaccag gcgtccacgg cgttaaaacc aatatccgcc gccgaccata cctggactgg
    40801 ctttttcgcc gcgccgagaa tggctttcat cgaaggaatt tgtggggagt tgatatcagt
    40861 ggaaacagca acaaccgcag gcagcggaat gcttaaggtt tcggtttcat cttccagttc
    40921 gcgctcaacg gtgagggtat ctgccgtcag ggagataatt ttgctgacgc cgttaactgc
    40981 cggaatattg aggatttcgc ccaccagcag accaacctgc tgggcataaa ggtcggaaga
    41041 accatcgcca cagaggatca gatcaaagcc tgctttctgg gcggctgcag ccagtgcgct
    41101 cgccgtttgt tgcggcagtg cctgctcgaa ctggtcatca atcaccacaa tcagttcatc
    41161 cgggccgcgc gatagcacat ctttacgccc tttggcgttg gtcagggctt taccgcccac
    41221 acttaaggct gtcacctgcg cctctgctgc ctgttgcttt agctggcaag ccgcttcaat
    41281 agcgttgaga tcgtattggc ttattttggc atcggctttg ctgaagtcta atgaaccatc
    41341 agcattattg accgcaatat cctgttcatc aggcacgcac ttatagcaag taataatctt
    41401 cattgcatct ccagaaatca tgaaggaaac gtcgttcata tcggtggtgt taattacaga
    41461 tatcacgccc tttaatttgg aaataaaact ttaatcacca atattgaaag tcagatgcta
    41521 tttttttacc cacaaaagaa tataacaaac tgattaataa ccaaaattaa cgaaaaacgc
    41581 cctctgttca ctcgaaacaa aaatgtgata ccaatcacag aatacagctt attgaatacc
    41641 cattatgagt tagccattaa cgcgtccacg atgttaataa taattatatt aaatgttaac
    41701 aaaaataaaa caaacgggaa cgcaaaataa atattcgttt tcacagtgga attaactcat
    41761 gaagaatgaa aagagaaaaa cgggaataga accgaaggtt ttctttccgc cattaataat
    41821 cgtcggcata ctttgttggc ttacagtcag agatctggat gcggcgaatg tcgttattaa
    41881 tgctgtattc agttacgtca ccaatgtatg gggatgggca tttgaatggt atatggtggt
    41941 gatgcttttc ggttggttct ggctggtgtt tggcccgtat gccaaaaagc gtttaggtaa
    42001 cgaaccgcca gaatttagca ccgccagttg gatctttatg atgttcgcct cctgtacgtc
    42061 tgctgccgta ctgttctggg gatcgattga gatctactac tacatctcca ccccgccgtt
    42121 tggcttagaa ccgaactcga caggagcgaa agagttgggg ctggcttaca gcttgttcca
    42181 ctggggacct ctgccgtggg ccacttacag cttcctttca gtcgccttcg cttacttctt
    42241 ctttgtccgc aaaatggaag tgattcgccc cagctcgaca ctggtgccgc tggcaggtga
    42301 aaaacacgcc aaagggttgt tcggtaccat cgtcgacaac ttctatctcg tcgccttgat
    42361 cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg gtgaccgagt gtatgcaatg
    42421 gttgtttggc attccgcata ccctgcaact ggacgctatc atcattacct gctggattat
    42481 cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg ccagtgacgt
    42541 gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc atcgtcagcg gtgccagctt
    42601 catcatgaac tacttcaccg attcggtggg gatgttgctg atgtatctgc cgcgcatgtt
    42661 gttctatacc gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg tgttctactg
    42721 ggcatggtgg gtgatttacg ccatccagat gagtatcttc ctcgcccgca tctcccgtgg
    42781 tcgtactgtg cgtgaactgt gcttcggcat ggtgatgggg ctgacagcat ccacctggat
    42841 cctgtggacc gtgctcggta gtaacactct gctgttgatg gataaaaaca tcatcaacat
    42901 tccaaatctg atcgaacagt acggtgtggc gcgcgccatc atcgaaacct gggccgctct
    42961 gccactcagc accgccacca tgtggggctt cttcatcctc tgctttattg ccaccgttac
    43021 gctggttaac gcctgctctt ataccctggc gatgtctact tgccgcgaag tacgcgatgg
    43081 tgaagaacca ccgctgctgg tgcgtatcgg ttggtcaatt ctggttggca ttatcggtat
    43141 tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc gccattatcg ccggaggatg
    43201 cccgctgttc ttcgtcaaca ttatggtgac gctctccttt attaaagacg cgaaacagaa
    43261 ctggaaagat taattaaccc ccaaaatatc aagaggttga aagatggatt ttaatttaaa
    43321 tgatgagcag gaactgtttg tcgccggtat ccgcgaactg atggccagcg aaaactggga
    43381 ggcctatttt gccgagtgcg accgtgacag cgtctacccg gaacgttttg tcaaagcact
    43441 ggcggatatg ggtatcgaca gtctgctgat ccctgaagag cacggtggtc tggacgcggg
    43501 gtttgttact ctcgccgccg tgtggatgga gctgggacgt ctgggggcac caacctatgt
    43561 actgtaccag ctgccgggcg ggttcaatac cttcctgcgc gaaggcacac aagagcagat
    43621 cgacaagatt atggctttcc gcggcaccgg taagcagatg tggaactcag cgattaccga
    43681 accgggcgcg ggctccgacg tgggtagcct gaaaacgact tatacccgta gaaatggtaa
    43741 gatttatctt aatggtagta agtgttttat taccagtagc gcctacaccc cgtacatcgt
    43801 ggtgatggcg cgcgacgggg cttctccgga caaacctgtc tacaccgaat ggtttgttga
    43861 tatgagcaaa ccgggcatca aagtgaccaa acttgagaag ctcggtctgc gtatggatag
    43921 ctgctgtgaa atcacctttg acgatgtgga actggacgag aaagacatgt tcggtcggga
    43981 aggtaacggc tttaaccgcg tcaaagaaga gttcgaccat gaacgtttcc tggtagccct
    44041 gaccaactac ggtacggcga tgtgcgcctt tgaagatgct gcgcgctacg ccaaccagcg
    44101 cgtgcagttt ggcgaggcta ttggtcgttt ccagttgatt caggaaaaat tcgcccacat
    44161 ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga aagcagacaa
    44221 cggcaccatc acctctggcg atgcagcgat gtgcaaatac ttctgcgcca atgcggcatt
    44281 tgaagtggtg gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg gcaaccaccg
    44341 catcagccgc ttctggcgtg acctgcgtgt agaccgcgtt tccggaggat ctgacgaaat
    44401 gcagatcctg acgctgggtc gtgcggtgct gaagcaatac cgctaagtcc gtgcgctgcc
    44461 tgatgcgacg ctgacgcgtc ttatcaggcc tacgggagca cgaatgtaag ccggataagg
    44521 cgtttacgcc gcatccggca gtcatgcgct acataaattt ttcaggagct aataatggat
    44581 catctaccca tgccgaaatt cggaccgctg gccggattgc gcgttgtctt ctccggtatc
    44641 gaaatcgccg gaccgtttgc cggacaaatg ttcgcagaat ggggcgcgga agttatttgg
    44701 atcgagaacg tcgcctgggc cgacaccatt cgcgttcaac cgaactaccc gcagctctcc
    44761 cgccgcaatt tgcacgcgct gtcgttaaat attttcaaag atgaaggccg cgaagcgttt
    44821 ctgaaattaa tggaaaccac cgatatcttc atcgaagcca gtaaaggtcc ggcctttgcc
    44881 cgtcgtggca ttaccgatga agtactgtgg cagcacaacc cgaaactggt tatcgctcac
    44941 ctgtccggtt ttggtcagta cggcaccgag gagtacacca atcttccggc ctataacacc
    45001 atcgcccagg ccttcagtgg ttacctgatt cagaacggtg atgttgacca gccaatgcct
    45061 gccttcccgt ataccgccga ttacttttct ggcctgaccg ccaccacggc ggcgctggca
    45121 gcactgcata aagtgcgtga aaccggtaaa ggcgaaagta tcgacatcgc catgtatgaa
    45181 gtgatgctgc gtatgggcca gtacttcatg atggattact tcaacggcgg cgaaatgtgc
    45241 ccgcgcatga gcaaaggtaa agatccctac tacgccggtt gcggtctgta taaatgtgcc
    45301 gacggctaca tcgtgatgga actggtgggc attacccaaa ttgaagagtg ctttaaagat
    45361 attggcctcg cacatctact cggtacgccg gaaatcccgg aaggcactca gcttatccac
    45421 cgtatcgaat gcccttacgg cccactggta gaagaaaaac tcgatgcctg gctggcggca
    45481 cataccatcg cagaagttaa agaacgcttt gccgaactga atatcgcctg tgccaaagta
    45541 ctgaccgtac cggaactgga aagcaatcca cagtatgttg cccgtgaatc aatcactcag
    45601 tggcaaacga tggatggtcg cacctgcaaa gggccgaaca tcatgccgaa attcaaaaat
    45661 aaccccggac aaatctggcg cggaatgccc tcacatggca tggacacggc tgccattttg
    45721 aaaaatatcg gctacagcga aaacgacatt caggagttgg tcagcaaagg tctggccaaa
    45781 gttgaggact aaacgcgtgg cgtcagtcgg cttgcctgac tagcgcccgc ccaataacaa
    45841 acgaacaaaa atggatagag gtgcaatgga tatcattggc ggacaacatc tacgtcaaat
    45901 gtgggacgat ctggcggacg tttacggtca taaaacggcg ctgatttgtg aatccagcgg
    45961 cggagtcgtt aaccggtata gttatcttga gttaaatcag gagattaacc gcacggcaaa
    46021 cctgttttat acgctgggga ttcgcaaagg caacaaggtt gcactacatc tcgacaactg
    46081 cccggaattt atcttttgct ggttcgggct ggcaaaaatt ggcgcgatta tggtgccgat
    46141 taacgcccgc ctgttacgcg aggaaagcga gtggatcttg caaaatagcc aggcgtgcct
    46201 gctggtgacc agtgcgcaat tttatcctat gtatcaacag attcagcagg aagatgccac
    46261 tcaattgcgg cacatttgcc tgacagatgt ggcacttccc gctgatgatg gagtgagttc
    46321 atttactcaa ctgaaaaatc aacaacctgc caccttgtgc tatgcaccgc cgctatcgac
    46381 tgacgatacg gcggaaattc tcttcacctc cggcaccacc tcccgaccga aaggtgtggt
    46441 gattacccat tacaacctgc gcttcgctgg atattactcc gcctggcagt gtgcactgcg
    46501 tgacgatgac gtctacatga cggtaatgcc tgcgtttcat atcgattgcc agtgtactgc
    46561 ggcgatggcg gcgttttctg ccggggccac ctttgtgctg gtcgagaaat acagcgcccg
    46621 cgccttctgg ggacaggcgc agaagtaccg cgccaccatt accgaatgta ttccgatgat
    46681 gatccgtacg ttgatggtgc agccgccttc agcgaacgat cggcaacacc gcctgcggga
    46741 agtgatgttt tatctcaact tgtcggagca ggaaaaagac gcattttgtg aacgcttcgg
    46801 cgttcgcttg ctgacgtctt atgggatgac ggaaaccatt gtgggcatta tcggcgatcg
    46861 ccctagcgat aaacgacgct ggccgtcgat tggtcgggcg gggttttgct acgaagcgga
    46921 gatccgcgac gatcacaatc gcccgctccc ggcaggtgag atcggtgaaa tctgtattaa
    46981 aggcgtacca gggaaaacca tcttcaaaga gtattttctc aacccgaaag ccactgcgaa
    47041 agtgctggaa gccgacggct ggctacatac cggcgatacc ggataccgcg acgaagaagg
    47101 ctttttttat ttcgtcgatc gccgctgcaa tatgatcaaa cgcggtggcg agaatgtctc
    47161 ctgcgtggag ctggaaaata tcatcgccac ccatccgaaa attcaggaca tcgtggttgt
    47221 gggtattaaa gattcgattc gcgatgaagc catcaaagca tttgtggtgc tgaatgaagg
    47281 tgaaacattg agcgaagagg aattttttcg cttctgcgaa caaaatatgg cgaaatttaa
    47341 agtgccctct tatctggaga tcagaaaaga tctgccacgt aattgctcgg ggaaaataat
    47401 tagaaagaat ctgaaataaa tgaaacgtca gggaactacc ctgccagcta ataatcacgc
    47461 cctaaaacaa tatgcatttt ttgcagggat gttgtcgtcc ctaaaaaagc aaaaatggag
    47521 aaaaggaatg agtgaatcat tacatctgac ccgcaatgga tcaattctgg aaattaccct
    47581 tgatcgccca aaagcgaatg ctattgatgc aaaaaccagc tttgaaatgg gcgaagtatt
    47641 tctaaatttc cgtgacgatc cgcaattacg tgtcgccatt attaccggtg ccggagagaa
    47701 attcttttcc gcgggctggg atttaaaagc ggcagcagaa ggcgaagcac cggatgctga
    47761 ctttggcccg ggtggttttg cgggattaac cgaaattttc aatctcgaca aaccagttat
    47821 cgcagctgtg aacggctacg cctttggcgg cggtttcgaa ctggcgctgg cggcagattt
    47881 tattgtttgt gccgataacg ccagcttcgc cctgccggaa gccaaactgg gcatcgttcc
    47941 tgacagcggc ggtgtgctgc gtctgccgaa gatcctgccg cctaccatcg tcaatgaaat
    48001 ggtgatgacc ggcagacgaa tgggcgcaga agaggcgctg cgttggggga tagtcaaccg
    48061 cgtggttagc caggcggaac tgatggataa cgcccgcgaa ctggctcagc agctggttaa
    48121 cagcgccccg ctggcgattg cggcgctgaa agagatctac cgcaccacca gcgaaatgcc
    48181 ggtagaagaa gcgtatcgct atattcgcag cggcgtgttg aaacactatc catcggttct
    48241 gcattcggaa gatgccattg aagggccgct ggcgtttgcc gagaagcgcg atccggtgtg
    48301 gaaaggacgt taacaaccgt gagctattac gcctttgagg ggttaattcc ggtggttcac
    48361 ccgacggcgt ttgtccatcc cagtgccgtc ttgattggcg atgtgattgt gggagccggt
    48421 gtctacatcg gcccactcgc ctcactgcgt ggtgactacg ggcggttgat cgtgcaagcg
    48481 ggagccaata ttcaggatgg ctgcattatg catggctact gcgacactga caccatcgtt
    48541 ggggaaaacg gccatatcgg gcatggagcg atcctgcatg gttgtgtgat tggtcgcgat
    48601 gcattggtcg ggatgaacag cgtaattatg gatggcgcgg tcattggcga agagagcatt
    48661 gttgccgcaa tgagctttgt caaagcaggc tttagtggcg agaaacgcca gttgttgatg
    48721 ggtacgccag cccgcgctgt acgcagtgtt agtgacgacg agttacactg gaaacggttg
    48781 aataccaaag agtatcagga tcttgtcggg cgctgtcatg catcgttaca tgaaacgcag
    48841 ccactgaggc aaatggagga aaatcgcccc cgtttgcagg ggacgacgga tgtgacgcct
    48901 aaacggtgat cttgccggat gcgacctggt tgctggataa gatgctacaa catcgtatct
    48961 ggcaagcagg acgcccggta tcaacgagat aaaattaacg acgcatactc tttgacaaca
    49021 tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg aacggcagga atacgcggtg
    49081 tctttcttac gccgactaat ttttcccgac tgttattgcg caggcgcgcg taaatttgct
    49141 catcgatatc gaccacttta accagtcgct gacactggca tccccgccct tccagcttat
    49201 tagggatcat cttaacttcg cagcttattt ctgtgacttc cgacagaata taagtcaggg
    49261 tattaaccgc cttgctgtgt tcaatatcga aatgaataga gatctctctt gctatcaccc
    49321 accgattttc agccatcatc cattcggcga ttaacaagta gagtggtttt tcaacatatc
    49381 cttcacacat acgaactcca gtttcacaaa ttaaaagtct agctatgact cgttttgaag
    49441 caattaaaca aggccatatt aaaattgtgg atatatcgat agtatgcaac ttcaccgtcg
    49501 ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca aaagatttac
    49561 ttaatgggca aaaaaaaacg gtcctcatca gagaaccgta ttagttattc gatcaaatta
    49621 gcggatgaaa aatatccgcc acgacacgct attatttgat ctgtgcgtgc atttcctgca
    49681 ccgaaattac tttttcagtc gcatcggcat tcagcgccat cgcggtagcg aaaccaccgt
    49741 tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc acgcgggagt
    49801 cttcaatcgc acgacggcct gaggtggtgt tgatgatgta ggtatattcg ccattcttga
    49861 tacggtcctg aatgtgcgga cggccttcat gcaccttgtt taccagacgc ggattgatac
    49921 ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag ccctgtttca
    49981 gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg gaaagcagcg
    50041 cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggcg aacgcttcag
    50101 cgaaggtgcg gcccacgccc atgacttccc cggtagagcg catttctggc cctaacagcg
    50161 ggtcaacgcc tgggaattta ttgaacggca gcaccacttc tttcaccgag tagtacggcg
    50221 gaataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc acacgcgccg
    50281 ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacagta cgcgccgcac
    50341 gcgggtttac ttcaatcagg taaacttcgt tgtttttcac cgcaaactgc acgttcatca
    50401 gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc acatcctgaa
    50461 tttcctgact taaggtgtac gctggcagcg aacatgcgga gtcaccggag tgcacacccg
    50521 cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg cagatggcat
    50581 ccacgtcaac ttctactgcg tcatcaagga aatgatccag caacactggc gcatcgttag
    50641 acacgctaac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag acgatttcca
    50701 tcgcccgacc gccgagaacg taagacggac gcaccaccag cgggtagcca atctcttttg
    50761 ccttctcaac tgccatttca atagcagtaa cggtggcgtt cgccggttgt ttcagtttca
    50821 gacggtcaac cgcatgctgg aagcgttcac ggtcttccgc acggtcgata gcatccgggc
    50881 tggtgccgat aaccggtacg ccagcggctt ccagtgcacg cgccagtttc agcggggtct
    50941 gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt tccagcacat
    51001 cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg gtagagacgg
    51061 tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcacgcagc gccagcgagg
    51121 cgtgtacgca gcagtagtcg aattcgatac cctgaccgat acggtttgga ccgccgccaa
    51181 gcaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct tcataagtgg
    51241 agtacatgta agcggtgtcg gtggcaaact ctgccgcaca ggtatccacg cgcttgtaga
    51301 ccgggtgcag gtcatattgg tcacgcagct tacggatttc cgcttcgcgt acgcccgcca
    51361 gttttgccaa gcgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg aattcagcgt
    51421 tcaggccagt gatgcccact tccgccactt tctcttccag acgcaccagt tcttcaatct
    51481 gtaccaggaa ccagcggtca atgttagtca ggttgaagac gccgtccaca gacaggcccg
    51541 cgcggaaagc atcggcgatg taccagatac gctctgcgcc tgcgtctttc agttcgcgac
    51601 ggattttggt taacgcttcc gggtcatcca ggctcacttt cgggtcgaag ccagttgcgc
    51661 ccacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga ccaatcgcca
    51721 tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcaccg gcgaattttt
    51781 cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg aaggaggccg
    51841 gagtacgtcc gccagtgatg tcgttcatca gttcgtctag ggtgtaaccc accgccagtt
    51901 tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc gaagaacggg
    51961 acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc accgcaaact
    52021 ggacattgga accaccggtt tcaacgccga tttcacgcag caccgccatc gaggcgttac
    52081 gcatgatttg atattctttg tcggtcagcg tttgggctgg cgcgacagtg atggagtcac
    52141 cggtgtggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg cagttgtcgt
    52201 ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac tcatcaatca
    52261 gcaactcttt ggtcggagag agatccagac cgcgggcgca aatttcttca aactcttcac
    52321 ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga ataatgcacg
    52381 ggaagcccac gtcagcggca acagccagcg cttcttccat cgtgtgtgcg ataccggaac
    52441 gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg tcttctgctt
    52501 tatcaatcgc atcggcagtg gcaccaatca tggtgacacc gaactcttcc aacacgccct
    52561 gacgttccag ctccagcgcg cagttcagcg ccgtctgacc gcccatggtt ggcagcaccg
    52621 cgtccgggcg ctctttttca ataatcttgc gtaccacttc ccagtgaatc ggctcgatgt
    52681 aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag ttcaccagaa
    52741 tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag tagtcaaact
    52801 cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt tttatatctg
    52861 tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt actgctcaat
    52921 taacttgata aagtggtcga acaacggcgc ggcgtcgtgt ggaccagggc tggcttcagg
    52981 gtgaccctgg aagctgaacg ccggcttatc gatgcgatga atgccctgta acgtaccgtc
    53041 gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat ccaccgcaaa
    53101 accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa ccggatggtt
    53161 gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca gcgccagcag
    53221 ctgatgaccg agacagatgc cgaataccgg aatatcggtt tcgaggaact tctggatggc
    53281 ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga agatgccgtc
    53341 tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg tcaggcgaca
    53401 gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat aagccacgac
    53461 gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccgccggtca acgtccagct
    53521 cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat ccatgccgtt
    53581 cagacctggg aacgcgcggg ctttttctaa cgccagcgcc gcatccgggt tatcgcctgc
    53641 gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct tacgggtatc
    53701 gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt cttcggtatt
    53761 acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg catgtacctg
    53821 agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat aagtaagagt
    53881 aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg tcattgaagt
    53941 attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac cgtgaaactg
    54001 ggttccgtct tccagaacca atagcgctga cttaatcaaa acaccctcca gagaatactc
    54061 actcacttta tttgcatatt aattcacagt gatgacgtaa atcaatgcaa atctgcttac
    54121 cgacgaattt ctggcaaacg gcggcattct ggagatatgg gcgctaaaag tcaacttaat
    54181 ggcaggattt tttatattat tttgtggctc atgcatgttt tacaggcatt aagcgacaaa
    54241 aacatcatct aacctgttct ggaaaacgct tgcgcagcaa gaagattgca tgaaataaaa
    54301 acgagcagga aaaagtggac caaatggtca aaacttacat taaaacaaca aaaatagaag
    54361 acgaaacatg taatttttgg cagttataga aaatttaaaa gcataacacc aaaaataa
//
LOCUS       DAZXUL010000011        53315 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZXUL010000011 DAZXUL010000000
VERSION     DAZXUL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53315
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.011"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(11..322)
                     /locus_tag="ACH3L1_000600"
     CDS             complement(11..322)
                     /locus_tag="ACH3L1_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFT4075485.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="ACH3L1_000601"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACH3L1_000601"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="ACH3L1_000602"
     CDS             701..1054
                     /locus_tag="ACH3L1_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFT4075486.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACH3L1_000603"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACH3L1_000603"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFT4075487.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="ACH3L1_000604"
     CDS             complement(2810..3280)
                     /locus_tag="ACH3L1_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFT4075488.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACH3L1_000605"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACH3L1_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /locus_tag="ACH3L1_000606"
     CDS             complement(4119..4337)
                     /locus_tag="ACH3L1_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFT4075489.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACH3L1_000607"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACH3L1_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFT4075490.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /gene="acrB"
                     /locus_tag="ACH3L1_000608"
     CDS             complement(5283..8432)
                     /gene="acrB"
                     /locus_tag="ACH3L1_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFT4075491.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="ACH3L1_000609"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="ACH3L1_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFT4075492.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="ACH3L1_000610"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="ACH3L1_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFT4075493.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="ACH3L1_000611"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="ACH3L1_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFT4075494.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="ACH3L1_000612"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="ACH3L1_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFT4075495.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="ACH3L1_000613"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="ACH3L1_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFT4075496.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="ACH3L1_000614"
     CDS             14911..15288
                     /locus_tag="ACH3L1_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFT4075497.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="ACH3L1_000615"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="ACH3L1_000615"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFT4075498.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="ACH3L1_000616"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="ACH3L1_000616"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFT4075499.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="ACH3L1_000616"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="ACH3L1_000617"
     CDS             18099..18428
                     /locus_tag="ACH3L1_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFT4075500.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="ACH3L1_000618"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="ACH3L1_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFT4075501.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="ACH3L1_000619"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="ACH3L1_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFT4075502.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="ACH3L1_000620"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="ACH3L1_000620"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFT4075503.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="ACH3L1_000621"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="ACH3L1_000621"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFT4075504.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="ACH3L1_000622"
                     /pseudo
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="ACH3L1_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="ACH3L1_000623"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="ACH3L1_000623"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFT4075505.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /gene="ybaL"
                     /locus_tag="ACH3L1_000624"
     CDS             complement(25481..27157)
                     /gene="ybaL"
                     /locus_tag="ACH3L1_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFT4075506.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="ACH3L1_000625"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="ACH3L1_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFT4075507.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="ACH3L1_000626"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="ACH3L1_000626"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFT4075508.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="ACH3L1_000627"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="ACH3L1_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFT4075509.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="ACH3L1_000628"
     CDS             complement(31205..31999)
                     /locus_tag="ACH3L1_000628"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFT4075510.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="ACH3L1_000629"
     CDS             32137..32478
                     /locus_tag="ACH3L1_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFT4075511.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="ACH3L1_000630"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="ACH3L1_000630"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFT4075512.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="ACH3L1_000631"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="ACH3L1_000631"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFT4075513.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="ACH3L1_000632"
     CDS             36394..37686
                     /locus_tag="ACH3L1_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFT4075514.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37717..37814
                     /locus_tag="ACH3L1_000633"
                     /pseudo
     CDS             37717..37814
                     /locus_tag="ACH3L1_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37811..38218
                     /gene="cueR"
                     /locus_tag="ACH3L1_000634"
     CDS             37811..38218
                     /gene="cueR"
                     /locus_tag="ACH3L1_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFT4075515.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="ACH3L1_000635"
     CDS             complement(38219..38677)
                     /locus_tag="ACH3L1_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFT4075516.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="ACH3L1_000636"
     CDS             complement(38674..39591)
                     /locus_tag="ACH3L1_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFT4075517.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="ACH3L1_000637"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="ACH3L1_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFT4075518.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="ACH3L1_000638"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="ACH3L1_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFT4075519.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="ACH3L1_000639"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="ACH3L1_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFT4075520.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="ACH3L1_000640"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="ACH3L1_000640"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFT4075521.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="ACH3L1_000641"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="ACH3L1_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /gene="ybbA"
                     /locus_tag="ACH3L1_000642"
     CDS             43550..44236
                     /gene="ybbA"
                     /locus_tag="ACH3L1_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFT4075522.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /gene="ybbP"
                     /locus_tag="ACH3L1_000643"
                     /pseudo
     CDS             44233..46648
                     /gene="ybbP"
                     /locus_tag="ACH3L1_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(46866..47021)
                     /gene="dinQ"
                     /locus_tag="ACH3L1_000644"
     CDS             complement(46866..47021)
                     /gene="dinQ"
                     /locus_tag="ACH3L1_000644"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFT4075523.1"
                     /translation="MKKIIVIGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="ACH3L1_000645"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="ACH3L1_000645"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFT4075524.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="ACH3L1_000646"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="ACH3L1_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFT4075525.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="ACH3L1_000647"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="ACH3L1_000647"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFT4075526.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="ACH3L1_000648"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="ACH3L1_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFT4075527.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="ACH3L1_000649"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="ACH3L1_000649"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFT4075528.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..53217
                     /locus_tag="ACH3L1_000650"
                     /pseudo
     CDS             52939..53217
                     /locus_tag="ACH3L1_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
     gene            complement(53231..>53315)
                     /locus_tag="ACH3L1_000651"
     CDS             complement(53231..>53315)
                     /locus_tag="ACH3L1_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFT4075529.1"
                     /translation="RKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT        12856 a        13713 c        14038 g        12708 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcacagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacctgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctagtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggctcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgttgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tatggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgactat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct atggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcggtctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg tttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata cagccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggagg cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 cggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattaatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc attcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt
    51121 ccaactaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct ggggggttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat tcgttggtaa
    53221 tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc ttgtcatgca
    53281 gctccaccga ttttgagaac gacagtgact tccgt
//
LOCUS       DAZXUL010000012        51474 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZXUL010000012 DAZXUL010000000
VERSION     DAZXUL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51474
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.012"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACH3L1_000652"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACH3L1_000652"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFT4075530.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACH3L1_000653"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACH3L1_000653"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFT4075531.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACH3L1_000654"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACH3L1_000654"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFT4075532.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACH3L1_000655"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACH3L1_000655"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFT4075533.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACH3L1_000656"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACH3L1_000656"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFT4075534.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACH3L1_000657"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACH3L1_000657"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFT4075535.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="ACH3L1_000658"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="ACH3L1_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFT4075536.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACH3L1_000659"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACH3L1_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFT4075537.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACH3L1_000660"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACH3L1_000660"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFT4075538.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACH3L1_000661"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACH3L1_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFT4075539.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACH3L1_000662"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACH3L1_000662"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFT4075540.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="ACH3L1_000663"
     CDS             complement(15665..16702)
                     /locus_tag="ACH3L1_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFT4075541.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="ACH3L1_000664"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="ACH3L1_000664"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFT4075542.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACH3L1_000665"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACH3L1_000665"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFT4075543.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="ACH3L1_000666"
     CDS             19444..19884
                     /locus_tag="ACH3L1_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFT4075544.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACH3L1_000667"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACH3L1_000667"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFT4075545.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21544)
                     /locus_tag="ACH3L1_000668"
                     /pseudo
     CDS             complement(20611..21544)
                     /locus_tag="ACH3L1_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACH3L1_000669"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACH3L1_000669"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFT4075546.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACH3L1_000670"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACH3L1_000670"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFT4075547.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACH3L1_000671"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACH3L1_000671"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFT4075548.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACH3L1_000672"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACH3L1_000672"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFT4075549.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACH3L1_000673"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACH3L1_000673"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFT4075550.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACH3L1_000674"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACH3L1_000674"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFT4075551.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACH3L1_000675"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACH3L1_000675"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFT4075552.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACH3L1_000676"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACH3L1_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFT4075553.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="ACH3L1_000677"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="ACH3L1_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFT4075554.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="ACH3L1_000678"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="ACH3L1_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFT4075555.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="ACH3L1_000679"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="ACH3L1_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFT4075556.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="ACH3L1_000680"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="ACH3L1_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFT4075557.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="ACH3L1_000681"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="ACH3L1_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFT4075558.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="ACH3L1_000682"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="ACH3L1_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFT4075559.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="ACH3L1_000683"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="ACH3L1_000683"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFT4075560.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="ACH3L1_000684"
     CDS             37412..37681
                     /locus_tag="ACH3L1_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFT4075561.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="ACH3L1_000685"
     CDS             complement(37684..38043)
                     /locus_tag="ACH3L1_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFT4075562.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="ACH3L1_000686"
     CDS             38184..38810
                     /locus_tag="ACH3L1_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFT4075563.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="ACH3L1_000687"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="ACH3L1_000687"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFT4075564.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="ACH3L1_000688"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="ACH3L1_000688"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFT4075565.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="ACH3L1_000689"
     CDS             41650..42315
                     /locus_tag="ACH3L1_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFT4075566.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="ACH3L1_000690"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="ACH3L1_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFT4075567.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="ACH3L1_000691"
     CDS             complement(42949..44166)
                     /locus_tag="ACH3L1_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4075568.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="ACH3L1_000692"
     CDS             44300..45349
                     /locus_tag="ACH3L1_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFT4075569.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="ACH3L1_000693"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="ACH3L1_000693"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFT4075570.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="ACH3L1_000694"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="ACH3L1_000694"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFT4075571.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="ACH3L1_000695"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="ACH3L1_000695"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFT4075572.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="ACH3L1_000696"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="ACH3L1_000696"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFT4075573.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="ACH3L1_000697"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="ACH3L1_000697"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFT4075574.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="ACH3L1_000698"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="ACH3L1_000698"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFT4075575.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="ACH3L1_000699"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="ACH3L1_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFT4075576.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
BASE COUNT        12063 a        14288 c        13202 g        11921 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacag cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcagcacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg aagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaaaatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcggg aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag cccttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat cagattaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aacagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctgacgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtaca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgtc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctaaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc acgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatca cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tata
//
LOCUS       DAZXUL010000013        50162 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZXUL010000013 DAZXUL010000000
VERSION     DAZXUL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50162
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.013"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            275..778
                     /locus_tag="ACH3L1_000700"
     CDS             275..778
                     /locus_tag="ACH3L1_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFT4075577.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(858..1109)
                     /gene="yecJ"
                     /locus_tag="ACH3L1_000701"
     CDS             complement(858..1109)
                     /gene="yecJ"
                     /locus_tag="ACH3L1_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFT4075578.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1224..1310)
                     /gene="azuC"
                     /locus_tag="ACH3L1_000702"
     CDS             complement(1224..1310)
                     /gene="azuC"
                     /locus_tag="ACH3L1_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFT4075579.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1573..1896
                     /locus_tag="ACH3L1_000703"
     CDS             1573..1896
                     /locus_tag="ACH3L1_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFT4075580.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            2067..2564
                     /gene="ftnA"
                     /locus_tag="ACH3L1_000704"
     CDS             2067..2564
                     /gene="ftnA"
                     /locus_tag="ACH3L1_000704"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFT4075581.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2601..2840)
                     /locus_tag="ACH3L1_000705"
     CDS             complement(2601..2840)
                     /locus_tag="ACH3L1_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFT4075582.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            3030..4241
                     /gene="tyrP"
                     /locus_tag="ACH3L1_000706"
     CDS             3030..4241
                     /gene="tyrP"
                     /locus_tag="ACH3L1_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFT4075583.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4303..4968)
                     /gene="yecA"
                     /locus_tag="ACH3L1_000707"
     CDS             complement(4303..4968)
                     /gene="yecA"
                     /locus_tag="ACH3L1_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFT4075584.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5164..5250)
                     /locus_tag="ACH3L1_000708"
     tRNA            complement(5164..5250)
                     /locus_tag="ACH3L1_000708"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5214..5216),aa:Leu,seq:taa)
     gene            complement(5263..5336)
                     /locus_tag="ACH3L1_000709"
     tRNA            complement(5263..5336)
                     /locus_tag="ACH3L1_000709"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5302..5304),aa:Cys,seq:gca)
     gene            complement(5391..5466)
                     /locus_tag="ACH3L1_000710"
     tRNA            complement(5391..5466)
                     /locus_tag="ACH3L1_000710"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5431..5433),aa:Gly,seq:gcc)
     gene            complement(5618..6166)
                     /gene="pgsA"
                     /locus_tag="ACH3L1_000711"
     CDS             complement(5618..6166)
                     /gene="pgsA"
                     /locus_tag="ACH3L1_000711"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFT4075585.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6223..8055)
                     /gene="uvrC"
                     /locus_tag="ACH3L1_000712"
     CDS             complement(6223..8055)
                     /gene="uvrC"
                     /locus_tag="ACH3L1_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFT4075586.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(8052..8708)
                     /gene="uvrY"
                     /locus_tag="ACH3L1_000713"
     CDS             complement(8052..8708)
                     /gene="uvrY"
                     /locus_tag="ACH3L1_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFT4075587.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8850..8921
                     /locus_tag="ACH3L1_000714"
                     /pseudo
     CDS             8850..8921
                     /locus_tag="ACH3L1_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            9004..9180
                     /gene="yecU"
                     /locus_tag="ACH3L1_000715"
     CDS             9004..9180
                     /gene="yecU"
                     /locus_tag="ACH3L1_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFT4075588.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            9167..9391
                     /gene="yecF"
                     /locus_tag="ACH3L1_000716"
     CDS             9167..9391
                     /gene="yecF"
                     /locus_tag="ACH3L1_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFT4075589.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9424..10044)
                     /gene="sdiA"
                     /locus_tag="ACH3L1_000717"
                     /pseudo
     CDS             complement(9424..10044)
                     /gene="sdiA"
                     /locus_tag="ACH3L1_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(10274..11026)
                     /gene="tcyN"
                     /locus_tag="ACH3L1_000718"
     CDS             complement(10274..11026)
                     /gene="tcyN"
                     /locus_tag="ACH3L1_000718"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFT4075590.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(11023..11691)
                     /gene="tcyL"
                     /locus_tag="ACH3L1_000719"
     CDS             complement(11023..11691)
                     /gene="tcyL"
                     /locus_tag="ACH3L1_000719"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFT4075591.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(11706..12692)
                     /gene="dcyD"
                     /locus_tag="ACH3L1_000720"
     CDS             complement(11706..12692)
                     /gene="dcyD"
                     /locus_tag="ACH3L1_000720"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFT4075592.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(12797..13597)
                     /gene="tcyJ"
                     /locus_tag="ACH3L1_000721"
     CDS             complement(12797..13597)
                     /gene="tcyJ"
                     /locus_tag="ACH3L1_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFT4075593.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(13685..14236)
                     /gene="fliZ"
                     /locus_tag="ACH3L1_000722"
     CDS             complement(13685..14236)
                     /gene="fliZ"
                     /locus_tag="ACH3L1_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFT4075594.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(14282..15001)
                     /gene="fliA"
                     /locus_tag="ACH3L1_000723"
                     /pseudo
     CDS             complement(14282..15001)
                     /gene="fliA"
                     /locus_tag="ACH3L1_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(15166..16818)
                     /gene="fliC"
                     /locus_tag="ACH3L1_000724"
     CDS             complement(15166..16818)
                     /gene="fliC"
                     /locus_tag="ACH3L1_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFT4075595.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            17084..18490
                     /gene="fliD"
                     /locus_tag="ACH3L1_000725"
     CDS             17084..18490
                     /gene="fliD"
                     /locus_tag="ACH3L1_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFT4075596.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            18515..18925
                     /gene="fliS"
                     /locus_tag="ACH3L1_000726"
     CDS             18515..18925
                     /gene="fliS"
                     /locus_tag="ACH3L1_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFT4075597.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            18925..19290
                     /gene="fliT"
                     /locus_tag="ACH3L1_000727"
     CDS             18925..19290
                     /gene="fliT"
                     /locus_tag="ACH3L1_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFT4075598.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            19368..20855
                     /gene="amyA"
                     /locus_tag="ACH3L1_000728"
     CDS             19368..20855
                     /gene="amyA"
                     /locus_tag="ACH3L1_000728"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFT4075599.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(20889..21302)
                     /gene="yedD"
                     /locus_tag="ACH3L1_000729"
     CDS             complement(20889..21302)
                     /gene="yedD"
                     /locus_tag="ACH3L1_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFT4075600.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPNWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            21489..22694
                     /gene="yedE"
                     /locus_tag="ACH3L1_000730"
     CDS             21489..22694
                     /gene="yedE"
                     /locus_tag="ACH3L1_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFT4075601.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            22691..22924
                     /gene="yedF"
                     /locus_tag="ACH3L1_000731"
     CDS             22691..22924
                     /gene="yedF"
                     /locus_tag="ACH3L1_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFT4075602.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            23031..23701
                     /locus_tag="ACH3L1_000732"
                     /pseudo
     CDS             23031..23701
                     /locus_tag="ACH3L1_000732"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(23741..24949)
                     /locus_tag="ACH3L1_000733"
                     /pseudo
     CDS             complement(23741..24949)
                     /locus_tag="ACH3L1_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            24957..25383
                     /gene="tnpA"
                     /locus_tag="ACH3L1_000734"
                     /pseudo
     CDS             24957..25383
                     /gene="tnpA"
                     /locus_tag="ACH3L1_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            25960..27042
                     /locus_tag="ACH3L1_000735"
     CDS             25960..27042
                     /locus_tag="ACH3L1_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFT4075603.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(27531..28329)
                     /locus_tag="ACH3L1_000736"
                     /pseudo
     CDS             complement(27531..28329)
                     /locus_tag="ACH3L1_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(28339..28890)
                     /gene="ybcL"
                     /locus_tag="ACH3L1_000737"
     CDS             complement(28339..28890)
                     /gene="ybcL"
                     /locus_tag="ACH3L1_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFT4075604.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(29059..29391)
                     /gene="emrE"
                     /locus_tag="ACH3L1_000738"
     CDS             complement(29059..29391)
                     /gene="emrE"
                     /locus_tag="ACH3L1_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFT4075605.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(29736..30050)
                     /gene="fliE"
                     /locus_tag="ACH3L1_000739"
     CDS             complement(29736..30050)
                     /gene="fliE"
                     /locus_tag="ACH3L1_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFT4075606.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            30264..31923
                     /gene="fliF"
                     /locus_tag="ACH3L1_000740"
                     /pseudo
     CDS             30264..31923
                     /gene="fliF"
                     /locus_tag="ACH3L1_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            31916..32911
                     /gene="fliG"
                     /locus_tag="ACH3L1_000741"
     CDS             31916..32911
                     /gene="fliG"
                     /locus_tag="ACH3L1_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFT4075607.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            32904..33590
                     /gene="fliH"
                     /locus_tag="ACH3L1_000742"
     CDS             32904..33590
                     /gene="fliH"
                     /locus_tag="ACH3L1_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFT4075608.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            33590..34963
                     /gene="fliI"
                     /locus_tag="ACH3L1_000743"
     CDS             33590..34963
                     /gene="fliI"
                     /locus_tag="ACH3L1_000743"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFT4075609.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            34985..35326
                     /gene="fliJ"
                     /locus_tag="ACH3L1_000744"
                     /pseudo
     CDS             34985..35326
                     /gene="fliJ"
                     /locus_tag="ACH3L1_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            35323..36450
                     /locus_tag="ACH3L1_000745"
     CDS             35323..36450
                     /locus_tag="ACH3L1_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFT4075610.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDLFA"
     gene            36555..37019
                     /gene="fliL"
                     /locus_tag="ACH3L1_000746"
     CDS             36555..37019
                     /gene="fliL"
                     /locus_tag="ACH3L1_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFT4075611.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            37024..38028
                     /gene="fliM"
                     /locus_tag="ACH3L1_000747"
     CDS             37024..38028
                     /gene="fliM"
                     /locus_tag="ACH3L1_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFT4075612.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            38025..38438
                     /gene="fliN"
                     /locus_tag="ACH3L1_000748"
     CDS             38025..38438
                     /gene="fliN"
                     /locus_tag="ACH3L1_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFT4075613.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            38441..38806
                     /gene="fliO"
                     /locus_tag="ACH3L1_000749"
     CDS             38441..38806
                     /gene="fliO"
                     /locus_tag="ACH3L1_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFT4075614.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            38806..39571
                     /gene="fliP"
                     /locus_tag="ACH3L1_000750"
                     /pseudo
     CDS             38806..39571
                     /gene="fliP"
                     /locus_tag="ACH3L1_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            39581..39850
                     /gene="fliQ"
                     /locus_tag="ACH3L1_000751"
     CDS             39581..39850
                     /gene="fliQ"
                     /locus_tag="ACH3L1_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFT4075615.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            39859..40644
                     /gene="fliR"
                     /locus_tag="ACH3L1_000752"
     CDS             39859..40644
                     /gene="fliR"
                     /locus_tag="ACH3L1_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFT4075616.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            40929..41552
                     /gene="rcsA"
                     /locus_tag="ACH3L1_000753"
     CDS             40929..41552
                     /gene="rcsA"
                     /locus_tag="ACH3L1_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFT4075617.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(41596..41784)
                     /gene="dsrB"
                     /locus_tag="ACH3L1_000754"
     CDS             complement(41596..41784)
                     /gene="dsrB"
                     /locus_tag="ACH3L1_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFT4075618.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            41947..42174
                     /gene="yodD"
                     /locus_tag="ACH3L1_000755"
     CDS             41947..42174
                     /gene="yodD"
                     /locus_tag="ACH3L1_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFT4075619.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            42472..43287
                     /locus_tag="ACH3L1_000756"
                     /pseudo
     CDS             42472..43287
                     /locus_tag="ACH3L1_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(43284..44978)
                     /gene="dgcQ"
                     /locus_tag="ACH3L1_000757"
                     /pseudo
     CDS             complement(43284..44978)
                     /gene="dgcQ"
                     /locus_tag="ACH3L1_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(45149..45331)
                     /locus_tag="ACH3L1_000758"
     CDS             complement(45149..45331)
                     /locus_tag="ACH3L1_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFT4075620.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(45410..46327)
                     /locus_tag="ACH3L1_000759"
     CDS             complement(45410..46327)
                     /locus_tag="ACH3L1_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFT4075621.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            46500..47420
                     /gene="yedA"
                     /locus_tag="ACH3L1_000760"
     CDS             46500..47420
                     /gene="yedA"
                     /locus_tag="ACH3L1_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFT4075622.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(47409..47879)
                     /gene="vsr"
                     /locus_tag="ACH3L1_000761"
     CDS             complement(47409..47879)
                     /gene="vsr"
                     /locus_tag="ACH3L1_000761"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFT4075623.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(47860..49278)
                     /gene="dcm"
                     /locus_tag="ACH3L1_000762"
     CDS             complement(47860..49278)
                     /gene="dcm"
                     /locus_tag="ACH3L1_000762"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFT4075624.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(49345..49846)
                     /locus_tag="ACH3L1_000763"
                     /pseudo
     CDS             complement(49345..49846)
                     /locus_tag="ACH3L1_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(49925..>50162)
                     /gene="drpB"
                     /locus_tag="ACH3L1_000764"
     CDS             complement(49925..>50162)
                     /gene="drpB"
                     /locus_tag="ACH3L1_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFT4075625.1"
                     /translation="WAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWL
                     GALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT        12519 a        12856 c        12195 g        12592 t
ORIGIN      
        1 ttcatcgatt aatatcaaca agatacaaaa agcactatca ttaaaattca ttgcatttac
       61 attgatttca ccaatgaaat gtaaaaatat ataaacttga tgatttaagc attttcttat
      121 aaccgttcag acgttattct tatttcagat catcgtcaga attgactcca cgatcacatt
      181 tcggaccggc agaaaggaat tattctgcaa acagtaatta tggtgttttg atttatcttg
      241 cacctctcca cttctggata taaggatatt aggtatggca accgctggaa tgcttctcaa
      301 actcaactct caaatgaacc gcgagtttta cgcatccaat ctctaccttc acctgagtaa
      361 ctggtgttct gaacagagtc tgaacggcac cgccactttc cttcgcgccc aggcacagag
      421 taatgtgacc caaatgatgc gcatgtttaa ctttatgaag agtgtcggcg ctacccccat
      481 cgttaaagcc attgatgttc caggtgaaaa actgaactct ctggaagaac tgttccaaaa
      541 aacgatggaa gaatacgagc aacgttcaag cactctggcg cagttagccg atgaagcgaa
      601 agaactgaat gatgattcaa ccgtcaattt cctgcgcgat ctggaaaaag aacagcagca
      661 tgatggcctg ttgctgcaaa ccattcttga tgaagtgcgc agtgcgaaac ttgcgggtat
      721 gtgccctgtg cagaccgacc aacatgttct gaatgtcgtg tcacaccagc tgcattgatc
      781 atacatcggc gctaatacat tgcgccgatg aagtttttga gaaaccgctg cctcatctgt
      841 ttgaagcagc ggttttttta atgggattca ccctgtgggg taaacttgag ttcaatcagc
      901 gcgatggctt tttggattgc ccgcatggtg accgggtctg cggcagcggg atgatgagta
      961 aagtcgatat tcttcagctg actggacatt ttttcacgaa cttcaacggg cgcgattaca
     1021 tcgagaacat ccagaatttg tttgataacc aactggcaag ctaccacatc agaaaccagt
     1081 tcctgatcgg cattcagcgg ctgggacatc gtaaactcct gatagcattt tgaaagccgt
     1141 tatagtagcg gcttcacatc ttcagcgata gtcacatcca ccgtcatcag gacacaaaaa
     1201 aacctgccgg agcaggtttt ttgttatcgg aacatattgc ctggcggtac gtctttgaac
     1261 gtcttgcaat agttattgaa catacttttc aggattttgc gcagtttcat cgcggcactc
     1321 cgaccatttg ttatacaggt gttattgtct ttgcgcttat aatatgacaa ccatcacaaa
     1381 aatcaatctt tatgtgatac aaatcacata aatacccctt taatgttata aaaatgataa
     1441 tcaaaaaaca gcccccctat ttctgacacc tacagatggc aagaaatagc gcctgccagg
     1501 cgtcttttcc ggccattgtc gcagcgctgt aacgcgtaaa atagtgcttt ctcttactct
     1561 tctggctgga ccatgagact tctgattctg actctttcat taataacgct ggcggggtgt
     1621 acggttactc gccaggccca tgtgagcgaa gttgacgcgg caacaggtat tgtacggttg
     1681 gtttatgacc aggctttttt gcagcatgcc cataccgatc gttatgtcag tcgcgggatc
     1741 gccgatcgcg catgccagca ggaaggctat acccacgcga tcccctttgg tcagccagta
     1801 ggcaactgta gcctttttgc gggttcctta tgtttgaata cggaattcac tttgtcatat
     1861 cagtgccatc attccgcctt ccctgtcttt ctctaaatat tttaatcagc gaggggatct
     1921 tcgctgatta aagaaatagc ggtaatgcgt tttattccca ttcgtatttt taataattga
     1981 agtttatatt ttaccttttg caaataataa aataacaaat tatagtgacg ccacagcaac
     2041 aaatataacc tttgtggagc actatcatgc tgaaaccaga aatgattgaa aaacttaatg
     2101 agcagatgaa cctggaactg tactcttcac tgctttatca gcaaatgagc gcctggtgca
     2161 gctatcatac cttcgaaggt gctgccgcgt tcctgcgccg tcacgcccag gaagagatga
     2221 cgcatatgca gcgtctgttt gattacctga ctgataccgg caatttaccg cgtattaata
     2281 ccgttgaatc tccgtttgct gaatattcct cacttgatga attattccag gaaacctata
     2341 aacacgaaca attaatcacc cagaaaatta acgaactggc tcatgctgca atgaccaatc
     2401 aggactaccc aacatttaat ttcctgcagt ggtatgtttc tgagcagcat gaagaagaga
     2461 aactgttcaa atcgattatt gataaattaa gcctggcagg caaaagcggc gaaggtctgt
     2521 attttatcga caaagaactc tctaccctcg acacacaaaa ctaatgctct ccggcggcag
     2581 gtttctgccg ccgtttcaaa tcagtgacgg caaatcttac tctgatcggt cgaaaaacct
     2641 tcttctgaag gtataaattt gccttttgct gccagaaacg ctaccagctc tcccgctgtc
     2701 accccttctg ccgagcacgt gtgaaaacgt gcctgttcac caaaacgcgc tttaatcgca
     2761 gcttccagac tggcatgcgt atattgctcg cctgattcaa tcatcatatt taacacttca
     2821 tgaccgtgaa tagagtccat cgttcctcct caaaaaaagc ctagcgtagc gattaccgct
     2881 tatgaagact ttgcgccagc gcaggactga atgcttttat tgtacattta tatttacaca
     2941 atatgtaacg tcggtttgac gaagcagccg ttatgcctta acctgcgccg cagatatcac
     3001 tcataaagat cgtcaggaca gaagaaagcg tgaaaaacag aaccctggga agtgttttta
     3061 tcgtggcggg aaccacaatt ggcgcaggca tgctggcaat gccgctggct gcggccggtg
     3121 ttggttttag cgttacgtta atcttgttga ttgggctttg ggcgttgatg tgctacacgg
     3181 cgctattact gctggaggtg taccagcatg ttccggcaga taccggtctg ggcacgctgg
     3241 caaaacgcta tctgggacgc tacggtcaat ggctgacggg cttcagtatg atgttcttaa
     3301 tgtatgctct gactgcagca tacatcagcg gtgccggtga attgttggcc tccagcatca
     3361 gcgactggac aggtatttca atgtcggcaa ccgctggcgt gctgttgttc acttttgttg
     3421 ccggtggcgt ggtttgtgtt ggaacatcgc tggtcgattt atttaaccgt tttctgttca
     3481 gcgccaaaat tatttttctg gtggtgatgc tggtattgct gctaccgcat attcacaaag
     3541 tgaatctttt aaccctgccg ttgcaacagg ggctggcact gtctgcaatc ccggtgattt
     3601 ttacgtcgtt tggttttcac ggtagcgtgc cgagtattgt cagctatatg gatggcaaca
     3661 ttcgtaagct acgctgggtg tttataatcg gtagtgccat ccccctggtg gcatatattt
     3721 tctggcaggt ggcgacgctt ggcagcattg attcaacaac ctttatggga ttgctggcta
     3781 atcatgctgg attaaacggg ctgttacagg cgttacgcga aatggtggcc tctccgcatg
     3841 ttgagctggc ggtgcattta tttgctgatt tagccctcgc cacgtcattt ctcggcgttg
     3901 cgttaggctt atttgattat ctggctgatt tgtttcagcg ttcaaatacc gttggtggac
     3961 gattgcaaac tggtgcaatt acctttctgc cgccgttggc gtttgcactg ttttatccac
     4021 gaggatttgt gatggcgctg ggttacgccg gtgtggcgct ggcggtactg gcattgatta
     4081 tcccttcact attgacctgg caaagcagaa agcacaatcc tcaggcgggt taccgggtca
     4141 aaggtggtcg tccggcgctg gtggtggtgt ttctctgtgg tattgctgtg attggcgtgc
     4201 aatttttgat tgcggcaggg ttgttaccag aagtggggtg atcagatagc ctcaaattcc
     4261 ttattgggtg ccagaattaa cgctgacacc caatgtgacc tcttaatgca ggcagcactg
     4321 cttaaatttc ttaccactgc cgcacggaca aggatcgtta cgccccggtt tctcttctgc
     4381 tttgatcggt tgctgaacag ctttttcctg cggatgcgcc atccagtatg catgtaaatc
     4441 aagcgccgcc agtcgaatgg catctacgct ctcttcaaac gcttctggcg acattttctc
     4501 tacccgctcg aagttttcct cagtaccgtg cagcgcaatc gcctccagcg ctggttttaa
     4561 agaatcgggc aacgttgacc agtcagaaag tgccacgccc cgcatatagc caaagcacca
     4621 ctcctcaaca atcgtcagct cgctgccatc aacttctcgc aagccgaata acggctcaaa
     4681 ctgctccggg aattcgttca gacgctctgc ggtatcggcc atatgttgaa aagccaggtt
     4741 cataaagcgc gtcatctctt tctctgacgc ccaacgcggc acatagtcag ccccacccca
     4801 cacggcaacc agccactgtt ctggttcaat ctcttgcgga gaactcaaca ccgccgtcaa
     4861 taaaccgtcc agctccgcca catcaaggat ggcgtggtca gtgttgtatt tggtcagaat
     4921 atcgtccagc cattccaact cactttcgtt taacggtccc gttttcatac gcttttcctt
     4981 gtggatctca actcgccagc acctatctta catgccggtc cgtatcagag atactttttg
     5041 agtggctttg ccggtgatta aaaattaagg agggtgtaac gacaagttgc aggcacaaaa
     5101 aaaccacccg aaggtggttt cacgacactg cttattactt tgattttatt cttatctttc
     5161 ccatggtacc cggagcggga cttgaacccg cacagcgcga acgccgaggg attttaaatc
     5221 ccttgtgtct accgattcca ccatccgggc tcgggaagaa agtggaggcg cgttccggag
     5281 tcgaaccgga ctagacggat ttgcaatccg ctacataacc gctttgttaa cgcgccaaat
     5341 tcttcaggcc tttcagccag acatccgctt gacgccgatg tcttttaaac tggagcggga
     5401 aacgagactc gaactcgaga cctcgacctt ggcaaggtcg tgctctacca actgagctat
     5461 tcccgcattc atcaagcaat cagttaatca cttgatttta ttatcgtctg gcaatcagtg
     5521 ccgccgttcg atgcgttgca ttctacttac ctggcgcgat gagtcaacga tatttttcac
     5581 cacttttgat cgtttgctga aaattacgcc gaaacgatca ctgatcaagc aaatctgcac
     5641 gcgcagcgct caaatattgc aacattgacc acagagtcag taccgcagcc acaaagaaaa
     5701 gtgcaatacc gacgtactca acccaaatgt tcggacgcca cagcagccag gccaacgcca
     5761 ccatctgggc agtggttttc actttcccaa tccaggagac ggccacgcta ctgcgtttac
     5821 ccaactccgc catccattcg cgtagcgcag aaataataat ttcacgggcg atcatcgttg
     5881 ccgccggtaa tgtcacccac cagctgtgat aatgctcggt taccagcacc atggcgatag
     5941 ccacgagaac tttatctgcc acagggtcaa ggaaagcacc aaaccgggta ctctggttcc
     6001 agcggcgtgc cagaaaacca tcgaaccagt cagtcaccgc cgcgacgcag aaaatgagcg
     6061 cggcggcaaa cggcgaccag gtgacaggca gataaaagac caatacaaag aatgggataa
     6121 ggatgacacg gaacagtgta agcaacgtag ggatattaaa ttgcataatg acgggtaact
     6181 atctgttgtc agtaagatta cccctatgtt gctacagaga catcaatgtt tcaacgacca
     6241 gaagatcttt tctgccagac cttgcgaaat acccggcact tttgcaattt cctcgacgct
     6301 ggcgttacgt aaaccttgca aaccgcccat atatttcaac aacatttgcc gacgttttgg
     6361 cccgacgcct tcaatggttt ccagggaact ggtatttttg accttcgccc gttttttacg
     6421 gtgcccgcca atcgcgtgat catgtgattc atcgcgaata tgctggataa catgcagcgc
     6481 gggtgaatct ggcggcaaac taaatccctc accttccggc tcaaagaaca gcgtttccag
     6541 tccagcctta cgatctgctc ctttggcaac gccaagtagc agcggatgat ttttatccca
     6601 tgagacatcc agttcggcga agacattttt cgcctgcgca agctggcctt tgccgccgtc
     6661 gataaggatc acatccggga tcttactgtc gtcaatggct ttaccataac gccgacgcag
     6721 cacctgattc atcgccgcat aatcatcgcc cggcgtgatg cctgtaatgt tatagcgccg
     6781 atactccgca cgcagcgggc cgttagcatc aaacaccaca caggaagcga cggtttgttc
     6841 gcccatggta tggctgatgt caaagcactc catccgcttc acttccggca atttcaacac
     6901 gctggcaagc gcggtcagtc gctggtgaac ggtagattgc tgcgaaagtt tgctggttaa
     6961 ggccgtcgcc gcattggtgc gcgcgagttt cagataacgc gccctatcgc cgcgaggttt
     7021 ggtttgaaca ttaatcttgc gtcccgccag ttctgaaagg gaatcggcga gcagcgtttt
     7081 atcgctaaga ttaaaatcga gcaggatctc acccggtaag gtgcgcatct ggctgccttg
     7141 taaatagaac tgacctacga aggtttctac cacctcgctc agttccgtac cgccaggcac
     7201 tttcgggaaa tagctgcggc tgccgagcac tttgccctga cgaatgaaca atacgtggac
     7261 acaagccatg cccgcatcga acgccacacc aataacgtca aggtcgtcgc cggtattgga
     7321 aacaaactgt ttttcggtga cgcgtcgcac cgcctgaatt tggtcgcgaa tacatgccgc
     7381 ttcttcaaac tccagattct ggctggcagt ttccatacgg ctaatgagtt gcgtaagcac
     7441 ctgatcatct ttgccagaca aaaacaggcg cacatactcg acctgctgag cgtattcttc
     7501 ttcactcacc agtccttcaa cgcacggtcc cagacagcgc cctatctggt attgcagaca
     7561 cggacgcgag cgattgcgat aaacactatt ttcgcactgg cgaatgggga aaatcttttg
     7621 cagtagcgcc agtgtttcac gtacggcata gccattcggg aacgggccga aatattcacc
     7681 tttggcatgc tttgcaccac gatgcatcgc cagacgcggg tgggtatcgc cactcaggaa
     7741 gataaaagga taagatttat catcgcgtag caaaacgttg taacgcggct gatagagttt
     7801 gatgtagttg tgttccagca acagcgcttc ggtttctgta tgcgtaaccg ttacatcaat
     7861 ttgctggatc tgggcgacca gcgcttcggt tttgcgcgaa gcgaggttgc tgcggaaata
     7921 gctggaaagc cgttttttca ggtctttcgc tttgccgaca tagataaccg taccaccagc
     7981 atcgtacatg cgataaacgc ctggctggct ggttacggtt tttaaaaacg cttttgcgtc
     8041 aaactgatca ctcactgact tgataatgtc tccgcattac acagaccatg gcgaattgcc
     8101 aggtgagtca gctcaacatc gccatgaatg tttagtttac tgaacatacg atagcggtag
     8161 ctgttcaccg ttttcggact gagattgagc tgttctgaga tctcattgac cttctggccc
     8221 ttagtgatca tcagcataat ctgcaattca cgttcagaca aactggcaaa tgggctttct
     8281 gttttttctg gttcgatctg gcttaacgcc atttgttgag cgatatcaga agcaatgtaa
     8341 cgctgccctg aatagacaga acgaatcgca ctcacgactt cctgcggagc cgcgcctttg
     8401 ctgaggtagc cagcagcacc tgcctgcatg actttcgctg gtaaagggtt ttctgtatgg
     8461 accgtaagca tgatgatttt aacatcagct gtggaacgcg cgattttacg cgtcgcctca
     8521 agaccgccaa tgcccggcat actcatgtcc attagcacca cgtcaacggc atttgtccgg
     8581 caccacttaa cggcgtcttc accgcacgat gcctctccga cgacttttat acccttaata
     8641 tcttccagaa tgcgtcgtat ccctgcgcgc accagttcgt ggtcatcaac aagtagaacg
     8701 ttgatcaaag gaatatctcc agaaataggg ataacgctac tgatagttag tcattcgtat
     8761 attagcggtt tttattgcaa ctttgaaacg ttaaaaatgg tgggtattcg atttttctct
     8821 cgtttttgtc aattcgattg tccacagtta tggtcacgcc cgtcaccaac ggcttaccgg
     8881 atcgtttcac agtaagccct acgatgaaat gtgacaaaaa ttgactttat tcagcaaaaa
     8941 tgaaaatcag cccgctgaat atgtaacatt aattaacccg aacaatacgg gcaaaaaaca
     9001 ttcatgataa aaatatttat tggtcattat attaacgtat tttatagcac tgccgatatc
     9061 acgctcaaaa aacaaccact gctattttta gcaaagctta tggtatactc cgccgcctta
     9121 acatttttca ccacaaattt tcattgcaac atgacgagga aaataaatga gtacgcctga
     9181 tttttctact gccgagaata atcaagaact ggcaaatgaa gtctcctgcc tgaaagcgat
     9241 gctgacgctg atgctgcagg cgatgggaca agctgacgcg ggccgcgtga tgttaaagat
     9301 ggaaaaacag cttgcgctga tcgaagacga aacccaggct gcagtatttt ccaaaacggt
     9361 taagcaaatt aaacaagcct accgtcagta atataaaacc ggctgagagc gtgcctttca
     9421 gccggttatt gacaaaatca tcgctatctc tgctgatgtc ttcccttccg ccgtccattt
     9481 cagaatttct ttttcgcgct tgctgaaatt catctctggc gtcatcacta tttcatcatt
     9541 taaacgcatc agagccatca gactttcgcg caccagtaac tgcattttta attgcagttc
     9601 atcactaaga atgggtattt cgcgcgtgct gcaacgggaa aaggacaaaa agcccagcgc
     9661 ccgatttggc agcattaaat actgagtgac accgcggcgt aaaccatgtg cgtgcgcggc
     9721 ttcccataat ggctgtgctt cgctgaataa gtcatcattc cacattaaat ggccctgact
     9781 aaagttttca gggttcagca caggatcaat tgcgagaaaa ttttttgcct gataataact
     9841 aacccacgac tcagggtaat tggtgtagaa agccacttta ggtcgcgtga atggtaccgg
     9901 gtggcgaaca cataacgaat agtaatcgta ctccagctgc tgagcctgaa gctctatttc
     9961 atggtaggcc tcttctgcgg cctccatcct ctgaaaacgc aacagcatcg tgcgacgcca
    10021 gctgaaaaaa tccgtatcct gcatagtaaa ccgcaacgcc cctgagagtg agtatcattt
    10081 ataatgataa ttcaaactat cacataaata agatttatat ataatttata ttattcaggc
    10141 aatgaattac ttttgcaagc catcgcattc tcttatgtta ttaatgagtt atgctgattt
    10201 gttaagcagt tttatcacgc ttgaaatggc gtcccgcccc gacaggtgaa tcgtcggggc
    10261 tgattttttc ttattattgc agcagaagct tctcgaggaa ctggcgggtg cgaggctgct
    10321 ggggatcggc aaataacgct tttgctgccc cctgctcgac tatccgcccc tggtccataa
    10381 agatcgcccg gtccgcaaca tcccgggcaa agctcatttc gtgtgtcaca atcaccatcg
    10441 tgcgcttttc ctgcgccagc tggcggatgg tgttcaggac ttcgcccacc agctctggat
    10501 ccagcgccga cgttggctcg tcaaacaaaa tcacctcagg acgcattgcc agtgcacgcg
    10561 caatcgcaac acgctgctgt tgaccgccag acaaacgacg tggatagctg gtttctttac
    10621 cggccagccc aactttcgcc agcagctcgc gagcgcgcgc cgtggcctct tctttcggtt
    10681 cgcctttgac gatcaccggc ccttcaataa tgttttccag caccgtacga tgcggaaaca
    10741 aattaaagtt ctggaagaca aacccgacgt gctgacgcaa ctggcgaatc agcgattttt
    10801 gctgacttaa tgaacgtgca gtatcaatag tgatatcgcc aacggtgatc gttcccgctt
    10861 ccggttgttc cagcagattt atgctacgta gcaatgtggt tttgccggaa ccactcggac
    10921 cgataattgc caccacttcg ccaggcttta cctcgaggtc gataccgtgc agcaccgtct
    10981 gaccgtggaa ttttttcacc aggttcttaa cttcaatggc actcattttg gttctctctc
    11041 ctggcgatta agttgattct caaaatggtt ctgcaacgtc gataacacag tcgccatgat
    11101 ccagtagatg agcgaagccg ccaggtacat ggtgaacacc tccagcgtgc gcgaggtaat
    11161 caactgcgcc tgacggaaca gctccggcac ctggattgtc gcggccagcg acgtatcttt
    11221 taccagactg ataaagctgt tcgacagcgg cggcaacgcc acccgcgccg cctgtggcaa
    11281 aatcgcgcga cgcatggtct gccacggcgt cataccaata ctggcagcgg cttcccactg
    11341 acctttatca atagaagaaa tggcggcgcg cagcgtttcg gcggcatagg cggcagtgtt
    11401 cagcgacaga ccaatcattg ctgacggaat gggatctaat tcgataccaa actgcggcag
    11461 gccgtaatag atcataaaca gttgtgcgat gagcggcgta ccacgaaaaa tagagatata
    11521 aaaacgcgcc agccagcgca cgggccagat tggcgacagg cgcatcagcg cgagaataaa
    11581 cccgagtagt aagccaaaaa acatgccgcc gatactgagt tgcagggtat acccagcccc
    11641 tttcaacagg aacggcaagg aatcaataac cagttgtata ctttcttgca tgagcgttcc
    11701 cggagctaaa cgtggggatg ataggcgaac agcgcaggcg cgccgccggt atgaataaac
    11761 agaatcggcc cttcatcttt gaagcgtttc tgactgatac cgtcaatcag ccccgccatc
    11821 gcttttccgg tatacacagg atcaagcaga atgccttcca gacgcgccag caatttcact
    11881 gcttccatgc cttcgtcgtt cggcacgccg tagccaggtg caaaatagtc atcccagagt
    11941 aaaatttccg ctgatgcggt cagctccagt tctttcgcaa tcgcctgttg taggttaacc
    12001 actttcggca attgatcggc aacggaacgc gacacggtca cgccaatcag ttcgctttca
    12061 ggcatcaggt gttccagccc aacagccagt ccggcgtgag ttccggcact gcccgatgcg
    12121 actaccaccg acgaaatatt aaccgcccct tcacactgtt gcgcgatttc cagcgcactc
    12181 tccacataac ctagcgcgcc aagagcatta gaaccgccaa ccggaatgac atacgggcga
    12241 aagccttgtg cttcgactcg cgtcgccagt tcttccagtt gggcattggg atcggtcagt
    12301 gcgtcgcaca tttcaatctg ggtattgaac agatccagca acaaacgatt gccgttggtt
    12361 aaatagtttt ctgcggttgt gccaatagga ttttccagca gcgccacgca gtgcagaccg
    12421 agtttcgccg cgactgcggc agtctggcgc acatggttag actggatcgc gccggcagta
    12481 atcagcgtat cggcaccttc gcgcagggca tctgccgcga gaaattccag cttacgtaat
    12541 ttattgccgc ccattgccat gggggtgacc tcatcccgtt tgatgaaaat ttcccgtcca
    12601 agataatcag aaaagcgcgg cagatattcg agcggcgttg gcgcgccgat aaactccaac
    12661 cgtggaaaac gggttaaatt atgcagtggc ataacagcct ccgatgtgtg ttgttgtgat
    12721 tttcttatta tgcacgctga aaacgcgtaa ataaaaaagg cgctagtgaa agcgcccttt
    12781 tttgtcatta tgctgattat ttggtcacat cagcaccaaa ccatttttcg gaaagagctt
    12841 gcagagtgcc gtctttttgc atttccgcaa ttgcatcatt caccgctttc agcaggtctt
    12901 catttccttt acgcagcgcc acgccagact cctgacggga gaatgcttca ccggttactg
    12961 ccagcgtatc gttggttttc ttcaccagat ccagcgccgc cagacgatca acgaggatcg
    13021 catcgatacg ccctacgcgc agatcctgat atttggtcgg gtcatcatca taggtacgca
    13081 catcgacacc ctgaacattc tgccgcagcc actcttcata gttggtgccc agaccgacac
    13141 ccactttttt gcctttcaga tcagcagccg ttttaatggt gccttcgtta ccttttttca
    13201 ccagcgcctg aataccggaa atggtgtaag gggtagagaa atcgtatttt ttcttgcgct
    13261 cgtcagaaat ggtgacctga ttaatcacca catcaatgcg tttagagtcc agcgacgcca
    13321 gcataccgtc ccatttggtc ggttttagtg acgcctcaac gccaagatgt tttgccagct
    13381 gttgggcaaa ttccacttca aaaccggtta atttgccgtc atccccctga aaactgaacg
    13441 gcggataagt gccttccagc cctaccagca gcgtgccgct ctctttgact ttattaagca
    13501 gaccttcatc tgcaaaactt ttaacgctca tgcccgcaac cagcgccacg gccatcacac
    13561 ccatcaatgc ctgacgtccc agatgtgcta atttcatatt caccccgaat gttgttatgt
    13621 ctgtttgcag tgtagagcca ttgtttgtaa acacaaaaac aactccgcta catcttattc
    13681 ttatttaata tatatcagaa gcaggcaggc tggaggattt ctgcacaagt ccggtacagg
    13741 tttggcgctg atagtgttga tacttccgta atgcgatgcg gtaattgttg gtgctggtgg
    13801 tgggcagcca cggggcgagg atttcatcaa ggaaaccgtc ctgcagctga tcgagggaaa
    13861 tattttgctc atgtagatga ttacccaaac ggcgcagacg aacgacatat tcacgcaccg
    13921 tacctgggct catttcagtt tgctcaaaaa gaaatttctt aaagccgata atatcgaaaa
    13981 aatcactctg cgttttgcaa tgtaaatcac cgcaaaatcg gcacaatgcc gcccatgatt
    14041 gttgttccgt ttgccagcct ttttcatcaa gcagcgtgtc caggcgggaa atctcgattt
    14101 tattcactat tttgccgtcg cgaactaagg taatgcgatc gagtaattta cggcaatgcg
    14161 cgcaatgggt ctggctgtgt ttaaagtctt taagatagcg gcttaatggc cgtcttttca
    14221 ggtgctgcac catcattaag aactcctggt agtcaaagtt aaagtgcggc atttactgac
    14281 gttataactt acccagttta gtgcgtaacc gtttaatgtc ctggctgtgt aactgactga
    14341 cccgcgattc cccgacctcc agcaccgcgc caatctcttt gagattcagc tcttcctggt
    14401 aatagagggt taataccagt ttttcgcgct ccggcaacgt ttcgatggct tccatcaccc
    14461 gctggcgcag attactgtcc agcagttgtt gtagcgggtt ttctcgctga tgatcatcag
    14521 taaccagttc gatgctatcg ccgtgctctt cacgccactc atcgtaggag aagagctggc
    14581 tgttattggt gtcgagcagc atttgacgat aatcggcaat atcgatccct aaacgttccg
    14641 ctacctcagt ttccgtggcg ttgcggccaa gttcctgctc cagttgccct attgcctgtg
    14701 ccacttcacg cgcgttgcgt cgcacgctgc gcggcaccca gtcacggctg cgaaggtcat
    14761 ccagcatagc gccacggata cgctacactg cgtaagttgt aaatgccgtt ccttgtaggg
    14821 cgtcatagcg ttcgacagca ttaagtaacc caatgccgcc cgcctgtagc agatcgtcaa
    14881 gttccacgct cgcgggcagt cgaacctgca ggcgcaatgc ttcgtgacgc accagcggga
    14941 cataacgctg ccacagcgag tgtttatcca ttacaccttc agcggtatag agtgaattca
    15001 cgataaacag ccctgcgtta tatgagttat cggcatgatt atccgtttct gcagggtttt
    15061 taatcggacg attagtgggt gaaatgaggg gttatttggg ggttacgcaa aaagccgaat
    15121 gtaaaaaacc ccgccggtgg cggggtttga gaggcattca gacgattaac cctgcagcag
    15181 agacagaacc tgctgcggta cctggttggc ttttgccagc acggagttac cggcctgctg
    15241 gataatctgc gctttcgaca tgttggacac ttcggtcgca tagtcggcgt cctgaatacg
    15301 ggactgcgct tcagacaggt tggtagtggt gttgttcagg ttggtgactg cggaatccaa
    15361 acggttttgc accgcaccca gggaggagcg gaatttgtcg atggagctga tggcttcatc
    15421 cagagctttc aggggatcgg ctgtcgtagc tgcttttgtt tcagcatctg tcgtcaattt
    15481 accatcagca tctttataaa ctgccttacc gctgccgtta gtcactgaac catcatcctg
    15541 taggtaaaaa tctacagtat cagatgtggt caacgcctcg ttgttatcaa cataaaccga
    15601 atcaacacct tttgttacag cgccagttgt gccgttgacg ctataacctg cagtagtgaa
    15661 atcaacattg tttcccgagg ttgatacagc tttatcgaaa gctgcctgat caacttttgc
    15721 acctgttact gaataagtaa tagtgtccgg cgtagtacta ttaccggtaa aggagataga
    15781 ggtaccgcca atgtcaatgg ttgtcgcttt ggtattattg gctgcaagtg tggacagact
    15841 agcctcagtc aaagaagcac cagagccgtt tttcgttaag cgcccagttg tatcaatgta
    15901 gagtttatct ccattgcttg ccgtaatttt accatcactg ctgatctgaa catcctgtgc
    15961 agaaccatcg atagtaatag tgcccttagc ggtatcacca actcccgggg tcaaatattt
    16021 ctgaacatcg gcagctgaag ccgttgtggt atcaaaagag tatgacttac ttgcactgtc
    16081 atacttgtaa ttcgttgcac tagccgccgt tccgaagccg ttattaatgg ttgcctgaac
    16141 agtatcacca tcactaactc cagccagcaa atcagacgct ttagcagcat cgtaacccgc
    16201 actcacagta tatttgttgc cgaccactgt tgcattagca gctaccaagt cagctttaga
    16261 tgcagcagta ttagccacag ccccgccacc attcacatta aacccattca gccccagcgt
    16321 atcagagtca attttcttca gatcaatagt gatagtctgg ccgtcattcg caccaacctg
    16381 aattttcatc gaaccgtctt ttgccagtac gttcacgccg ttgaactggg tctgaccgga
    16441 tacgcggtca atttcgtcga gacgggattt gatttcgtcc tgaatggagt ccagatccga
    16501 atcagagtta gtcccggtag aagcctgaac cgtcagctca cggatacgct gtaagttgtt
    16561 gttgatttcg gacagcgcgc cttcagtggt ctgtgcaaca gaaataccgt cgttggcgtt
    16621 acgtgcagcc tgagtcaggc ctttaatgtt agaagtaaaa cggttagcaa tcgcctgacc
    16681 cgcggcgtca tccttcgcgc tgttaatacg caagccagaa gacagacgct cgatagaact
    16741 cgacagcgca gactggttct tgttgatatt attttgagtg atcagcgaga ggctgttggt
    16801 attaatgact tgtgccatga ttcgttatcc tatattgcaa gtcgttgatt acgttttggg
    16861 tttccacccg tcggctcaat cgccgtcaac cctgttatcg tctgtcgtaa aacaaccttt
    16921 agaatttttt tcacaaacag ccattttttg ttagtcgacg aaatactctt ttctctgccc
    16981 cttattcccg ctattaaaaa aaacaattaa acgtaaactt tgcgcaattc aggccgataa
    17041 ccccggtatt cgttttacgt gtcgaaagat aaaaggaaat cgcatggcaa gtatttcatc
    17101 gctgggagtc gggtcaggtc tggatttaag ttcaatcctt gatagcctca ccgccgcgca
    17161 aaaagcgacg ctaaccccta tttcaaatca gcaatcgtcg tttaccgcta aacttagcgc
    17221 ctacggtacg ctgaaaagcg cgctgacgac tttccagacc gccaatactg cattgtctaa
    17281 agccgatctt ttttccgcca ccagcaccac cagcagcacc accgcgttca gtgccaccac
    17341 cgcgggtaac gccatcgccg ggaaatacac catcagcgtc acccatctgg cgcaggcgca
    17401 aaccctgacc acgcgcacca ccagagatga tacgaaaacg gcgatcgcca ccagcgacag
    17461 caaactcacc attcaacaag gcgacgacaa agatccgatt accattgata tcagcgcggc
    17521 taactcatcg ttaagcggga tccgtgatgc catcaacaac gcaaaagcgg gcgtaagtgc
    17581 gagcatcatt aacgtgggta acggtgaata tcgtctgtca gtcacatcaa atgacaccgg
    17641 ccttgataat gcgatgacac tctcggtcag cggtgatgat gcgctacaaa gttttatggg
    17701 ctatgacgcc agtgccagca gcaacggtat ggaggtctcg gtcgccgccc agaatgcgca
    17761 gctgacggtc aacaacgtcg ccatcgagaa cagcagcaac actatcagcg acgcgctgga
    17821 aaacatcact ctgaacctga acgatgtcac cacgggcaac cagacgctaa ccatcactca
    17881 ggacacctcc aaagtgcaaa cggcgattaa agactgggtg aatgcctata actcgctaat
    17941 agataccttc agcagcctga ccaaatacac cgccgtagat gcgggagctg atagccagag
    18001 ttctagcaat ggcgcactgc tcggcgactc cacgctgcgg acgattcaga cgcagttgaa
    18061 gtcgatgctg agtaataccg tcagttcttc cagctataaa acgctggcgc agattggtat
    18121 cacgaccgat cccagcgatg gcaaactgga actggatgcc gacaaactca ccgctgcact
    18181 gaaaaaagat gccagcggcg taggtgcatt gattgttggc gatggtaaaa aaaccggcat
    18241 cacaaccacc atcggcagca acctgaccag ttggctttcg acaacgggca ttattaaagc
    18301 cgctaccgat ggcgttagta aaaccctgaa taagttaact aaagactaca acgccgccag
    18361 cgatcgcatt gatgcgcagg tcgcgcgcta caaagaacaa tttacccaac tggacgtttt
    18421 aatgacctcg ttaaacagca ccagcagcta cttaacgcag cagttcgaaa acaacagtaa
    18481 ttccaagtaa tcattattca tcgggagaca ggtcatgtac gcggcaaaag gcacccaggc
    18541 ctatgcacaa attggcgtcg aaagcaccgt aatgagcgcc agccaacagc agttggtcac
    18601 catgctattt gatggagtgc tgagcgcact ggttagagcg cgactgttta tgcaggacaa
    18661 caatcagcaa ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg agaacggact
    18721 gcgggtgagt cttgatgaag agagcaaaga cgaattaacc caaaacttaa ttgctcttta
    18781 tagctatatg gtcaggcgtt tgctgcaagc caatttacgc aacgatgtct cagcagtcga
    18841 agaggtggaa gcattaatgc gtaatattgc cgatgcctgg aaagagtctt tactctcccc
    18901 ttcttcgatt caggacccag tctgatgaac aatgcaccgc atttgtattt cgcctggcaa
    18961 caactcgtcg aaaaaagcca gctcatgtta cgcctggcaa cggaagaaca atgggacgaa
    19021 ctcatcgcta gcgaaatggc gtatgtgaat gcggtgcagg agatcgcaca cctgactgaa
    19081 gaggttgccc cgtccactac gatgcaggag cagctccgcc cgatgctgca cctgattctc
    19141 gacaacgaaa gcaaggtaaa gcagttatta cagattcgga tggatgaact ggcgaaactg
    19201 gtcggtcagt catcggtgca aaaatcggtg ttaagtgcct atggcgatca gggcggcttt
    19261 gtgctggctc cgcaggataa cctcttttga atcttaatga gccgatggct cgcgaataat
    19321 ccgattacgg ctacgcttct aatattcccc ttgaatggag tcgaagaatg cgtaatccca
    19381 cgctgttaca atgttttcac tggtattacc cggaaggcgg taagctctgg cctgaactgg
    19441 ccgagcgcgc agacggtttt aatgatattg gtatcaatat ggtctggttg ccgcccgcct
    19501 ataaaggcgc atcgggcggc tattcggtcg gctacgactc ctatgattta tttgatctag
    19561 gtgagtttga tcagaaaggc agcatcccta ctaaatatgg tgataaagca caactgctgg
    19621 ccgccattga cgccctgaaa cgtaatgaca ttgcggtgct gctggacgtg gtagtcaacc
    19681 acaaaatggg cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat gctgatgacc
    19741 gtacgcaaat tgacgaagaa atcattgagt gtgaaggctg gacgcgttac accttccccg
    19801 ctcgtgccgg gcaatactcg cagtttatct gggatttcaa atgttttagc ggtatcgacc
    19861 atatcgaaca ccctgatgaa gatggcattt ttaaaattgt taacgactat accggagaag
    19921 gctggaacga tcaggttgat gatgaactcg gtaatttcga ttatctgatg ggtgagaata
    19981 tcgattttcg caatcatgcc gtgacggaag agattaaata ctgggcgcgc tgggtgatgg
    20041 aacaaacgca atgcgacggc tttcgtcttg atgcggtcaa acatattccg gcatggtttt
    20101 ataaagagtg gatcgaacac gtacaggaag ttgcgccaaa gccgctgttt attgtggctg
    20161 aatgctggtc gcatgaagtc gataaactgc aaacgtatat tgatcaggtg gaaggcaaaa
    20221 ccatgctgtt tgatgcgccg ctgcagatga aattccatga agcatcgcgc atgggtcgcg
    20281 actacgacat gacgcagatc ttcactggga cgctggtgga agccgatcct tttcacgccg
    20341 taaccctcgt tgccaaccac gacacccaac cgttgcaagc cctcgaagca ccggtcgaac
    20401 cgtggtttaa accgctggcg tatgccttaa ttttgttgcg ggaaaatggc gttccttcgg
    20461 tgttctatcc ggacctctac ggtgcacatt acgaagatgt cggtggtgac gggcaaacct
    20521 atccgataga tatgccaata atcgaacagc ttgatgagtt aattctcgcc cgtcagcgtt
    20581 tcgcccacgg tgtacagaca ttatttttcg accatccgaa ctgcattgcc tttagccgca
    20641 gtggcaccga cgaatatccc ggctgcgtgg tggtcatgtc gaacggagat gatggcgaaa
    20701 aaaccattca tctgggagag aattacggca ataaaacctg gcgtgatttt ctggggaacc
    20761 ggcaagagag cgtagtgaca gacgaaaacg gcgaagcgac cttcttttgc aacggcggca
    20821 gcgtcagcgt gtgggttatc gaagaggtga tttaaatcca tccccggcgg caagccgggg
    20881 agatttcatt acggcagtgg cgtcggtaac ggcgctttat ccagcgcggc ggcacactct
    20941 gccgtcgggc gatccacacg ctccatcgtc ataccatcgt actcaatggt gttaccgtca
    21001 cgctcaacct cgtacagctc acgttttacg gtaacgttag tgagatcatc agacatcaat
    21061 gtcaacttac ccggcacggc aatcccccgc tgccactgac ggcaatccaa ggtatcgccc
    21121 tctttagtga caatcaggct gcctatcgct tccggactga ccagcgcacg ttgcggccct
    21181 ttggtctgcc agtagcccgc cagccagttc ggcgcggggg ttttcacgac attgttatag
    21241 ttttcgactt cggcacagcc cgccagcaac atgagcgcgc ctgcaattgc gagttttttc
    21301 atcattcgtc ctgcatgaga agaaaagagg attgtggcat taaagctctg atgtcgccag
    21361 cctttccagg ttcagagaga aatttgatct aactattatt tttgtgaatt attaccccga
    21421 aaataaattt gcatggtgta tgattcgcgg ctcttgtttc aaaccttctg agttcagagg
    21481 ctacattcat gtcatggcag caattcaaac acgcctggtt gattaaattc tgggcaccca
    21541 tccctgcggt catcgcggcg ggtattctct ccacttacta ttttggcatt actggcacct
    21601 tttgggctgt cacgggtgaa tttacccgtt ggggcggtca gctcctgcag ctgtttggcg
    21661 tccatgctga agagtggggt tattttaaaa ttatccatct ggaaggatcg ccattaaccc
    21721 ggatcgacgg catgatgatc ctcggtatgt ttggcggctg ctttgctgct gcgctgtggg
    21781 ccaacaatgt caaactacgc atgccacgca gccgtatccg cattatgcag gccatcattg
    21841 gcggcattat cgccggtttt ggcgcgcgtc tggcaatggg ctgtaacctg gcggcgttct
    21901 ttaccggcat tcctcagttc tcgctgcatg cctggttctt tgccatcgcc actgccattg
    21961 gttcatggtt tggcgcgcgc tttaccctgc tgcccatctt ccgtattccc gtgaaaatgc
    22021 agaaagtttc tgccgcctca ccgctgacgc aaaaaccgga tcaggcgcgg cgtcgttttc
    22081 gtctcgggat gctggtcttt ttcggcatgc tgggctgggc gctgctcacg gcgatgaacc
    22141 aaccaaaact ggggctggca atgctgtttg gcgtcggctt tggtttactg attgaacgtg
    22201 cgcaaatctg ctttacttcg gcgttccgcg atatgtggat caccggacgt acccatatgg
    22261 cgaaagcaat cattatcggt atggcggtga gtgccatcgg gatcttcagt tacgtacagt
    22321 taggcgttga acctaaaatc atgtgggcgg gaccaaatgc ggtaattggt ggtttacttt
    22381 ttggttttgg catcgtgctg gcaggcggct gcgaaaccgg ctggatgtac cgcgcggtag
    22441 aaggccaggt gcactactgg tgggtcggtc tgggcaacgt gatcggctca acgattctgg
    22501 cgtattactg ggatgatttc gctccggcgc tggccaccga ctgggacaaa atcaacctgc
    22561 tgaaaacttt tggtccgatg ggaggcctgc tggtgacata tttgctgctg tttgctgcgc
    22621 tgatgttgat tatcggctgg gaaaaacgct tcttccgccg tgaggcaccg cagactgcta
    22681 aggagatcgc atgaaaaata tcgttcctga ttaccgcctt gatatggtgg gtgaaccctg
    22741 cccttatccg gcagtcgcaa cccttgaggc gatgccgcaa ttaaaaaaag gtgagatcct
    22801 ggaagtggtg agcgactgtc cgcagtcgat caataatatt ccactggatg cgcgtaatca
    22861 cggctacacg gtgctggata ttcagcaaga cgggccgacc attcgttatt taattcagaa
    22921 gtaatccctc attcccgctg tggcccacag cgggaacatc gttacctctg acacatagat
    22981 tttacccgcc ccctctccta ttcttttcag tatgatctca ggaggttgct atgtgtggac
    23041 gctttgccca atcccaaacg cgtgaggatt accttgcgct tctcgcggaa gatattgaac
    23101 gcgatattcc ctacgatccc gaacccattg gcagatacaa tgtcgcgccg ggaaccaaag
    23161 ttcttctgct cagtgaacgt gatgaacacc ttcatctgga tccggttttc tggggatatg
    23221 ctcccggatg gtgggataaa ccgccgctga ttaacgcccg cgtagaaact gcctcaacca
    23281 gtcgcatgtt taaaccgctc tggcaacatg gtcgggcaat ctgttttgcc gatggctggt
    23341 ttgagtggaa aaaagaaggc gacaaaaaaa gccttatttt atctatcgcg ctgacggaca
    23401 acctgttttt atggccgcga taggtagcat accgtttgag cgtggtgacg aagccgaagg
    23461 atttttgatc gtcactgcgg cggcagatca aggtctggta gatattcatg accgccgccc
    23521 gctggttctg tcgccagaag ctgcacggga atggatgcgg caagacattg gtggtaaaga
    23581 agcctcagaa atcgctacca gaagctgtgt gccagcaaac cagttcacct ggcaccctgt
    23641 gtcgcgcacg gtgggtaatg tcaaaaacca gggcgcggag ttaattcaac ctgtttgttg
    23701 acatcctcca cgccctgaat gacgaggacc cctgctacgt tcaggctgtc gcctgaatca
    23761 tttcggtggg ttcctgcttc aacgagcggc ctgactgcac catctctcca caggcaagaa
    23821 cggcgtgccc cgccgctaaa atgttacgag ctccatttac atcggcgttc gctgtatatc
    23881 cacatacctg gcatctgaat ttactttgtg acaggcgatt ttctttcgct gtatgaccac
    23941 agcacgcgca acgctggctt gtgtacgctg gcggaacagc aagcacctga ccgccacgcc
    24001 agagctgctt gtactcaagc tggcggcgca tttcatacca gccctgatcc agtatcgaac
    24061 ggtttaaacc tgattttgcc cggacattgc gccccggctg gctgaccgta cccgctgctg
    24121 actttgacat gttgctgacc ttcaaatcct caatgactat cattacgtgg tttttgctga
    24181 cggtcgttgt gactttgtga aggtagtccc tgcggatatt tgcgatacag gaatgcagtc
    24241 gctgtatttt gcgtttctgc ttctgccagt tgttgctgaa tttgaccttg cggcttaact
    24301 gtcgctgaag tctcgccagc gtcttctggt ttttctgaaa actgtttaca ggctcaaaga
    24361 ctgtgccatc tgacagcgag gcaagtttag ccacgccagc atccagcccg accattgatg
    24421 ctgaagggtg aaccggagtt gatacttcac tttctgcctg aatactgatg taccacttac
    24481 cgcaggactg gctgacagtg acatttttca caacacccgt gacctgacgg ctgttccggt
    24541 agcgcatcca gccaagtttt ggcagaaaaa tacggctgtt ttcctgagcg agcttaacgc
    24601 cctgcggata gcggaatgca tcattctgtc accgcttttt gaatcagggg aacgccgagc
    24661 gcttctggaa aaagtttttg caggcctgct cagggtcttt cagtgactgt tgcaatggct
    24721 gtgacggagc atctttaatc cattgcgttt cagtggcatt tttccactca accaatcagg
    24781 aagccatttt cgcgtaaggg atgtctttgt taccggcctt atgattctca ttctgacgtg
    24841 ccagagtacg actgaaaacg aaacgacatg cgcctgcgaa gcgcctcatt tcacgcgcct
    24901 gttgaccacc gggtcttaac cggaatttga atacctgtag tcgtttcaca gggcaaatta
    24961 tactttggtc tgtgaaaaaa actgatatcc gacgcggaag gcactgtgtt ttcctgatgc
    25021 atgctcaccg ggtctttgtt accaagtacc gacgccagat ttttgaccat gacgcgacag
    25081 aaaaactacg cacttacttt tcaaatgtat gtgcttattt tgaagctgaa ctggttgaaa
    25141 tggatggcga accagatcac gtccatctgt taattaacta tccccccaaa ctggcgatat
    25201 ccagtctggt aaacaaacag cctcaaaggc gtatcgggta ggttactgcg acgggatcga
    25261 ccagatattg ctgccaggta ttaccgcaaa gatgttttgt ggcgtcccgg ctattttgcc
    25321 agtagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca acagcaaaca
    25381 cctggtcaga tgaaaaaacg agccttatat ccccgccctg aaggacgggc ttttacggtg
    25441 cactggataa gcctggagat tatctacacc tgcatggaca tcattgtact atgccccgta
    25501 catgctccat cacatcctca acatcaaagt attagagcct tgcaaaccat tatgcgtctc
    25561 agtttgtctc atctatctta cgcaaaaaaa atagaactaa tatcaagcgg cgctatattg
    25621 cgtattaaat aaaaccacct caattacatt aataaatcta aataaatatc atgcaaataa
    25681 tttccatggt tatttttagg ctatttaaat taaaaaaata aattatgagt caatcataaa
    25741 taacaacctt taatataatt tgactaccaa atttatattc gttcacaaaa tagtcatgca
    25801 ttgtgtgcaa tagaaacagt tcagataaaa atagggatct acttcacaat ttaaatgaga
    25861 aactaaaact tacatcttga aataatctca ttgattggat gaatatttgt agtgcagtga
    25921 catcattttt aaataatagt tcaaataaaa gggctcacga tgaaaaaatt aacagtggca
    25981 atttctgctg tagctgcatc agtactgatg gcgatgtctg ctcaggcagc agagatttac
    26041 aataaagaca gtaacaagct ggatctgtac ggaaaagtta atgctaagca ctacttctct
    26101 tctaacgatg cagatgatgg cgacacaact tatgttcgtc tgggcttcaa aggcgaaact
    26161 caaatcaacg atcaactgac tggtttcggt cagtgggaat atgaattcaa aggcaaccgt
    26221 gctgaatctc aaggttcttc caaagataaa acccgtcttg ccttcgctgg cctgaaattc
    26281 ggtgactacg gctccatcga ttatggccgt aactacggtg tagcatacga tatcggtgca
    26341 tggaccgacg ttctgccaga attcggtggc gatacctgga cccaaacaga tgtgttcatg
    26401 actggtcgca ccacaggtgt tgcaacctat cgtaacaacg acttctttgg tctggttgat
    26461 ggtctgaact ttgctgctca gtatcagggt aaaaatgacc gcactgacgt aactgaagct
    26521 aacggtgatg gtttcggttt ctccactact tatgagtatg aaggattcgg tgtaggtgca
    26581 acctatgcta aatctgaccg cactaatgat caggttatct acggtaacaa cagcctgaat
    26641 gcatctggtc aaaatgctga agtatgggca gctggtctga aatatgatgc gaacaacatc
    26701 tatctggcta ccacctattc tgaaacccag aacatgactg tttttggtaa taaccatatt
    26761 gccaacaaag cacaaaactt cgaagtagtt gcacaatatc agttcgactt cggtctgcgt
    26821 ccgtccgttg cttacctgca atctaaagga aaagacttgg gtgcgtgggg tgatcaggac
    26881 ctgattgaat atattgatgt aggtgcaacc tattacttca acaaaaatat gtccactttt
    26941 gttgattaca aaatcaacct gattgataag agcgatttca cgaaagcatc tggcgttgct
    27001 accgatgata tcgttgctgt aggattggtt taccagttct aatctgatta ctcaaagata
    27061 tgttgtggga ggctttgcct ccccaacata taagtggcgc tctcaagcca ctttccttag
    27121 aagcactatc ttgcttctga ctatataaat taccctttat tttctgggac gtttcaacgc
    27181 cccattttta ataatactca gtgaacaatt aacgtatcaa ttagatttaa taacaacgat
    27241 atccgtacct aacctgatat ctaatgtcat aagcatccct ttaattatcc cctcaacctc
    27301 ttgtaaattt cccactcttg agctccacat agcccaaacg ccggatgcaa aaaaaatcat
    27361 acgtgcatct ctgaaattgt gatggaggaa gtgagtatca attatatgtg cttataaacg
    27421 ttcccgattg gcacagaagt caccggattg ttcaggctcc aataggttga gtatgtcgta
    27481 tttattttcc aaaataaaaa tcgatgctgt tgctccttaa taaaatactt ttactctctg
    27541 gacgcttttc ttagctctct tggtgttata ttaaaacgat tatggaacct ttcagtaaaa
    27601 cgagaaggac acttgtaacc attttccctg gcaatctcgc tgatgggttt tactgtcgtt
    27661 tgtatagcag gacaacgcat tatttaacct cacgtcgtcc aatatacttt gaaaacttat
    27721 tccctcgctt gccagacggc gatgcaatgt ggaaacagaa atgtagagat atctcgctac
    27781 cttgtttgct gtccattttg ttctaggttc ggatagcatt aggttataac aacgacttat
    27841 taatgattgt ttactatctg ataaaagtaa acaattaaca tgatgtgctc ctaatgaaag
    27901 tagaacgccc attgctaagt gttcctgaat ttgcgttgag aagcctcggg aaatagatgt
    27961 ttttagttgc tcccaacaat atattaactc ggggttctga ggtaagcaga aacctgtcgt
    28021 gttacgaatt tgatcagtta tcgcataaaa tttttgaaac ttttcaatta gatcaatggg
    28081 ataataaagc atttctgcaa gataaagacc tgcttctgga taattcacaa tataaaattc
    28141 atatccacag ggaaataata ttatctgatt attatcaaca gttagactat gcgtctccca
    28201 attgataact ttttttccct gacggatacg acataaagct ggcataagag gcttaaccct
    28261 gtgaatctca tgatgtttat gcatccgtat ttcttcaatc tttaggttag acttatctct
    28321 tgccagcata ctcttaccct attttatctc ataaactggt gttatctcag cggttgcaat
    28381 tttattggca ttaagcatat aaccaactaa cgctccgctg gaattagaat ccacaggaat
    28441 cttatcagtt tttagagccc atactttaaa ctggtaatga tggggtttat ctcctttggg
    28501 aggacatgct ccaccaaatc ctgcataacc aaaatcattt cggccttgaa cagcaccagt
    28561 cggaagtttt gtaccatcac gtcttcctgc atcagtgggc aaatatgtta cagttgctgg
    28621 aatattagca acagtccagt gccaccatcc actgcctgta ggagcatctg gatcatacac
    28681 agttacggca aagcttttgg tgccttcagg agcaccagac caggttaatg agggcgatgt
    28741 attaccacct tcacacccaa atccagaaaa tacatgagac gttgtaagtt gctctcctgt
    28801 ttttatttca ttactagtga cctgaaatgc tgcggcctgc gcagaaaatg ttatgaatgc
    28861 caatacactt gaaacgataa gttttttcat aaaaacctca ttgttgtgac ctatcgttat
    28921 tttatttgat gtgccttgat ctcattatgc ataaaggcgc aatgatcgcg caaatgcaat
    28981 cataattata aaggtgcccc gaacattagc cataatatgc acaaatcaga ttcctgttat
    29041 ctcaaggaga attatttttt aatgcggtgc acttcgtgac aacaagttaa tgactaacac
    29101 accggcacaa atcaacatca tgcctatgat agctggcagg tccagtcgtt gaccaaaaaa
    29161 tccccaggaa agcaaactaa tcagaacgat accgactcct gaccagatag cataagcaat
    29221 ccctgtaggt atataagcca gcgtctgagc taataaccag aatgatgcac aatagcaaat
    29281 aattgtgcca acagagggcc ataaccgtgt aaacccctct gaaaacttca ttaaggttgt
    29341 accaatgacc tctgcaagta ttgcgccacc aagataaata taagggttca tagcatattc
    29401 tttcctgttc atactggaga gaattgtact acagtttgaa cacaactcac cgttttcatc
    29461 ctggtgtatg tgtatccaag gggctctggc gtggcaagag ataaaggcag gaacaataat
    29521 gatcagaagg aaccccaggc taacgaatga gttcactctt cctgggagca aagcgaagac
    29581 aaaacatgtt atcgcgttta cggatcgtgg atggcagaaa acaaccagga tcaggtatcc
    29641 attttaaacc agaaattgag tgagtttgcc ccatccatgc cccacgctgt gggatcggat
    29701 ataattaaac aagcatagtc aacaggttac acaacctaca cctgcatgga catcacttcc
    29761 tgatacgccg ccaccagctt attacgcacc tgaatcccca tttgcataga aactgaggct
    29821 ttttgcatat cggtcatcac atcgtttagc gccacgccgg gttcaccgag ggtgaatttt
    29881 tctgcctgcg tgcgggcgac tgtttgtgta tcgcttatgc gatcgagcgc ggcgtgcagc
    29941 tgcccggcaa aactaatggt cggttgcggc agtgattcct gcgcacgcgc actcatcgcc
    30001 gtagcctgta actggctgat aaccccttca atcccctgta tcgctgacat tttcgtctcc
    30061 cggataattt ctggtaacaa agcctaccag taagtcaata agacaaaggc gctaaatagc
    30121 aacaaaaaaa ctggtttatt ggcggataga aaaaacgaaa gcacaaataa tgggagcgtc
    30181 aatttttcga gtgtgctgac ccgagagtga gtcttgttcc actttgccaa taacgccgtc
    30241 cataatcagc cacgaagtgc gcgatgaatg cgactgcagc ccagacaaaa tctcttgagt
    30301 ggcttaatcg cctgcgtgcg aatccgaaaa ttccattgat tgttaccggt tccgcggcag
    30361 tggcggtcat ggtcgcactg atcctgtggg cgaaagcccc cgactaccgc acattattca
    30421 gcaatctttc cgatcaggat ggtggcgcaa ttgtcagcca actgacgcaa atgaatattc
    30481 cttaccgctt cagcgaagcc agcggcgcta ttgaggttcc ggcagataaa gttcacgaac
    30541 tgcgcctggc gcaacaaggt ttgcccaaag gcggcgcggt cggtttcgaa ctgctcgatc
    30601 aggaaaagtt tggtatcagc cagttcagcg aacaggtgaa ttatcagcgg gcgctggaag
    30661 gcgagctttc tcgtaccatc gaaactatcg gcccggtaaa aggggcgcgc gtacatctgg
    30721 caatgccgaa accgtcttta ttcgtccgtg aacaaaaatc cccttctgca tcggtgacgg
    30781 taaatctgtt acccggccgc gcactcgatg aagggcaaat tagcgctatt gtacatctgg
    30841 tttccagcgc ggttgctggt ctgccgcctg gaaacgtcac gctggtggat cagggcgggc
    30901 atctgttaac ccagtccaat accagcgggc gcgatcttaa tgacgctcag ttgaaatatg
    30961 ccagcgatgt cgaaggccgt attcagcagc gtattgaagc gatcctgtca cctattgttg
    31021 gtaacggtaa tattcacgcc taggtcacgg cgcaactgga cttcgccagt aaagaacaaa
    31081 cggaagaaca gtatcgccct aacggtgatg aatctcatgc ggcgcttcgt tcacgccagc
    31141 ttaatgagag cgagcaaagc ggttccggtt atccgggcgg cgtaccgggg gcgttgtcga
    31201 atcaaccggc acctgcgaat aacgcgccaa tcagcacgcc tccgacaaat caaaataacc
    31261 gccagcagca ggcgagcacc accagcaata gtgggccgcg tagcacacag cggaatgaaa
    31321 ccagtaacta cgaagtcgat cgcaccattc gtcataccaa aatgaacgtg ggcgatgtgc
    31381 aacgtctgtc agtcgcggta gtggtgaatt acaaaacctt gccagacggt aaaccattgc
    31441 ctctcagcaa cgaacagatg aagcaaattg aagctctgac ccgcgaggcg atgggctttt
    31501 ctgaaaaacg cggtgattcg ctcaatgttg ttaactcgcc gttcaatagc agtgacgaaa
    31561 gcggcggagc actgccgttt tggcaacagc aagtgtttat cgatcagttg ctggctgccg
    31621 gtcgctggtt gctggttgct ggtactgctg gtggcgtggt tgctgtggcg gaaagcggta
    31681 cgcccgcagc taacacgtcg cgccgaggcg gtgaagactg tgcagcaaca ggcgcaggcc
    31741 cgcgaggaag tggaagatgc ggtggaagtc cgtctgagca aagacgaaca actccaacaa
    31801 cggcgcgcta accaacgact gggggcagaa gtcatgagcc agcgtatccg tgaaatgtct
    31861 gataacgatc cgcgcgtggt ggcgctggtc attcgccagt ggataaataa cgatcatgag
    31921 taacctgaca ggcaccgata aaagcgtcat cctgctgatg accattggcg aagaccgggc
    31981 ggcagaggtg ttcaagcacc tctcccagcg cgaagtgcaa accctgagcg ctgcaatggc
    32041 gaacgtcacg cagatctcca acaagcagct aaccgatgtg ctggcggagt ttgagcaaga
    32101 agctgaacag tttgctgcac tgaatatcaa cgccaacgat tatctgcgtt cggtattggt
    32161 caaagctctg ggtgaagaac gtgccgccag cctgctggaa gatattctcg aaacccgcga
    32221 taccgccagc ggtattgaaa cgctcaactt tatggagccg cagagcgccg ccgatctgat
    32281 tcgcgatgag catccgcaaa ttatcgccac cattctggtg catctgaggc gcgcccaggc
    32341 cgccgatatt ctggcgttgt tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc
    32401 cacctttggc ggcgtgcagc cagccgcgct ggccgaactg accgaagtac tgaatggctt
    32461 gctcgacggt cagaatctca agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat
    32521 tatcaacctg atgaaaacgc agcaggaaga agccgttatt accgccgtgc gtgaattcga
    32581 cggcgagctg gcgcagaaaa tcatcgacga gatgttcctg ttcgagaatc tggtggatgt
    32641 cgatgatcgc agcattcagc gtctgttgca ggaagtggat tccgaatcgc tgctgatcgc
    32701 gctgaaaggg gccgagcagc cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc
    32761 cgccgatatt ctgcgcgacg atctcgccaa gcgtggtccg gtgcgtctgt cgcaggtcga
    32821 aaacgaacag aaagcgattc tgctgattgt gcgccgtctt gccgaaactg gcgagatggt
    32881 gattggcagc ggcgaggata cctatgtctg ataatctgcc gtggaaaacc tggacgccgg
    32941 acgatctcgc gccaccaccg gcagagtttg tgccaatggt cgagtcggaa gaaaccatta
    33001 ttgaagaggc cgaacccagc cttgagcagc aactggcgca actgcaaatg caggcccacg
    33061 agcagggtta tcaggcgggg attgccgaag gtcgccagca aggtcacgaa cagggctatc
    33121 aggaaggact ggcccagggg ctggaacaag gtctggcaga ggcgaaggct caacaagcgc
    33181 caattcatgc ccggatgcag caactggtca gcgaatttca aactaccctt gatgcgcttg
    33241 atagtgtgat agcttcgcgc ctgatgcaga tggcgctgga ggcagcacgt caggtgatcg
    33301 gtcagacacc aacggtggat aactcggcac tgatcaaaca gatccaacag ttgttgcagc
    33361 aagaaccgtt attcagcggg aaaccacagc tgcgcgtaca cccggatgat ctgcaacgtg
    33421 tggatgatat gctcggcgca actttaagtt tgcatggctg gcgcttgcgg ggcgatccca
    33481 ccctccaccc tggcggatgt aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg
    33541 ccactcgctg gcaagaactc tgccgtctgg cagcaccagg agtggtgtaa tgaccacgcg
    33601 cctgactcgc tggctaacca cgctggataa ctttgaagcc aaaatggcgc agttgcctgc
    33661 ggtacgtcgc tacgggcgat taacccgcgc taccgggctg gtgctggaag ccaccggatt
    33721 acaattgccg ctcggcgcaa cctgtgtcat tgagcgccag aacggcagcg aaacgcacga
    33781 agtagaaagc gaagtcgttg gctttaacgg tcaacggctg tttttaatgc cgctggagga
    33841 agtggaaggt gtcctgcccg gcgcgcgtgt atatgccaaa aacatttcgg cagaagggct
    33901 gcaaagcggc aagcagttgc cgctcggtcc ggcgttatta ggtcgtgttc tggacggtag
    33961 cggtaaaccg ctcgatggcc tgcccttccc cgatacgacg gaaaccggtg cgctgattac
    34021 cccgccattt aacccgttgc aacgtacacc gattgaacat gtgctggaca ccggcgtgcg
    34081 cccaatcaat gccctgctta ccgtcggacg cgggcagcgt atggggctgt ttgccgggtc
    34141 gggcgttggt aaaagtgtgc tgctggggat gatggcacgc tacacccgcg ccgatgtcat
    34201 tgtcgtgggt ttgattggtg aacgtgggcg cgaagtaaaa gattttattg agaatatcct
    34261 cggtgccgaa ggacgtgcac gctcagtggt gattgccgct ccggcggatg tttctccgct
    34321 cctgcgaatg caaggtgccg cctatgccac gcgcattgcc gaagattttc gcgatcgtgg
    34381 tcagcatgtg ttgctgatta tggactccct cacccgctac gcgatggccc agcgtgaaat
    34441 tgcgctggcg attggcgaac cacctgccac taaaggttat ccaccgtcgg tgtttgccaa
    34501 attaccggca ctggtcgagc gtgccggtaa tggcattagc ggcggcggct cgattaccgc
    34561 gttttatacc gtgctcaccg aaggcgatga ccagcaggac cccattgctg actccgcgcg
    34621 ggcaatcctc gacggtcaca ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc
    34681 ggctatcgat attgaagcgt cgatcagtcg cgcaatgacg gcgttgatca gtgagcaaca
    34741 ttacgcgcga gtgcgcacct tcaaacagct gttgtcgagt tttcagcgta accgcgatct
    34801 ggttagcgtc ggcgcgtatg ccaaaggcag cgatccgatg ctcgataaag ccatcgccct
    34861 gtggccgcag ctggagggct atttgcaaca aggcattttt gaacgcgcgg actgggaagc
    34921 gtctctccag gggctggagc gtattttccc gacagtgtca taacccagga gataacggca
    34981 gatggcggaa gaacagctca aaatgttgat cgattatcag aatgaatatc gcaataacct
    35041 caatagcgat atgagtgccg ggatgaccag caaccgctgg atcaactatc agcagtttat
    35101 ccagacgctg gaaaaagcca ttactcagca tcgccagcaa cttaatcagt ggacgcagaa
    35161 agttgacatt gccctgaaca gctggcgaga aaagaaacaa cgtttgcagg cctggcagac
    35221 actgcaggaa cggcaatcca cggcggcact gcttgcagaa aaccgcctcg atcagaaaaa
    35281 gatggatgag ttcgcccagc gcgccgccat gaggaaacct gaatgattcg cttagcgccc
    35341 ttgattaccg ccgacgttga caccaccaca ttgcctggcg gcaaagccag cgatgctgca
    35401 caagattttc tcacgttgtt gagcgaagca ttagcaggcg agacaacaac tgacaaagcg
    35461 gccccccagt tgctggtagc aacagataag cccacgacaa aaggcgagcc gctggtcagc
    35521 gatattcttg ccgatgcgca acaagcggat ttactgatcc ctgtggatga aacacttcct
    35581 gtcatcaacg acgaacaatc cacatcaaca ccgttaacca ccgctcagac gatgacgttg
    35641 gctgcggtgg ctgacaaaaa tacgacaaaa gacgaaaaag cggatgatct taatgaagac
    35701 gtcaccgcaa gtctgagcgc cctttttgcg atgttgccgg gttttgacaa tacgcccaaa
    35761 gtgactgatg caccgtcaac cgtgttaccg gcagagaaac caacgctctt cacaaaactg
    35821 acttctgcgc aactcactac agcacagcct gatgacgccc ccggcacacc agctcagcca
    35881 ttaacaccgc tggtagcaga agcccagagt aaagcggaag tcatcagcac gccttcgccg
    35941 gtgaccgctg acgccagccc gctaatcact ccacaccaga cacagccact gcccaccgtc
    36001 gccgcgcctg ttttgagtgc accgctgggt tctcacgaat ggcaacaatc attgagccag
    36061 catatttcgc tgttcacccg ccaggggcaa caaagtgcag agttgcgtct gcacccacag
    36121 gatttaggtg aagtgcaaat ttccctcaaa gtggatgata accaggcgca gatccagatg
    36181 atttcaccgc atcagcacgt acgcgccgcc ctggaagcag cgctgccggt actgcgtacg
    36241 cagctggccg aaagtggcat tcagttaggg caaagcaaca ttagtggcga aagctttagt
    36301 ggtcagcagc aggccgcttc ccagcaacag caaagccaac gcacagcaaa ccatgaacct
    36361 ctggcggggg aagacgacga tacgcttccg gttcccgtct ctttacaagg gcgtgtaaca
    36421 ggtaacagcg gcgttgatct tttcgcctaa cgtcagaggt agcaccgtaa tccgcgtctt
    36481 ttccccgctt tgttgcgctc aagacgcagg ataattagcc gataagcagt agcgacacag
    36541 gaagaccgca acacatgact gattacgcga taagcaagaa aagcaagcga tcgctttgga
    36601 tcccgattct ggtattcatt accctcgcgg cctgtgccag cgcaggttac agctactggc
    36661 attcgcatca ggttgccgct gacgacaaag cgcagcaacg cgtcgtgccc tccccggtct
    36721 tctatgcgct ggataccttt acggtcaatt tgggcgatgc ggatcgcgtg ctttatatcg
    36781 gcataaccct gcgactgaaa gatgaagcaa cccgctcgcg gctgagtgag tatttgccgg
    36841 aagtccgtag tcgcttgctg ttactgtttt cacgtcagga tgctgccgta ctggcgacag
    36901 aagaaggcaa gaaaaacctg attgccgaga ttaaaaccac actttccacc ccgcttgttg
    36961 ccgggcaacc gaaacaggat gtcaccgacg tgctgtatac cgcttttatt ctgcgataac
    37021 gacatgggcg atagtattct ttctcaagct gaaattgatg cgctgttgaa tggtgacagc
    37081 gaagtcaaag acgaaccgac agccagtatt agcggcgaaa gtgacattcg tccgtacgat
    37141 ccgaataccc aacgacgggt tgtgcgcgaa cgtttgcagg cgctggaaat cattaatgag
    37201 cgctttgccc gccattttcg tatggggctg ttcaacctgc tgcgccgtag cccggacatt
    37261 accgtcgggg ccatccgcat tcagccgtac catgaatttg cccgcaacct gccggtgccg
    37321 accaacctga acctaatcca cctgaaaccg ctacgcggca cagggctggt agtgttctcg
    37381 ccgagtctgg tgtttatcgc cgtggataac ctgtttggcg gcgatggacg cttcccgacc
    37441 aaagtggaag gtcgcgagtt tacccatact gaacagcgcg tcatcaaccg tatgttgaag
    37501 ctggcgctgg aaggctatag cgacgcctgg aaagcgatta atccgctgga agttgagtac
    37561 gtgcgttcgg aaatgcaggt ggaatttacc aatatcacca cctcgccgaa cgacattgtg
    37621 gttaacacgc cgttccatgt ggagattggc aacctgaccg gcgaatttaa tatctgcctg
    37681 ccattcagca tgatcgagcc gctacgggaa ttgttggtta acccgccgct ggaaaactcg
    37741 cgtaatgaag atcagaactg gcgcgataac ctggtgcgcc aggtgcagca ttcacagctg
    37801 gaactggtgg ccaactttgc cgatatctcg ctacgcctgt cgcagatttt aaaactgaaa
    37861 cccggcgacg tcctgccgat agaaaaaccc gatcgcatca tcgcccatgt tgacggcgtc
    37921 ccggttctga ccagtcagta cggtactctc aacggtcagt atgcgttacg gatagaacat
    37981 ttgattaacc cgattttaaa ttctctgaac gaggaacagc ccaaatgagt gacatgaata
    38041 atccggccga tgacaacaac ggcgcaatgg acgatctgtg ggctgaagcg ttgagcgagc
    38101 aaaaatcaac cagcgaaaaa agcgctgccg acgcggtgtt ccagcaattt ggcggtggtg
    38161 atgtcagcgg aacgttgcag gatatcgacc tgattatgga tattccggtc aagctgaccg
    38221 tcgagctggg ccgtacgcgg atgaccatca aagagctgtt gcgtctgacg caagggtcag
    38281 tcgtggcgct ggacggtctg gcgggcgaac cgctggatat tctgatcaac ggttatttaa
    38341 tcgcccaggg cgaagtggtg gttgttgccg ataaatatgg cgtgcggatc accgatatca
    38401 ttaccccgtc cgagcggatg cgccgcctga gccgttagtg atgaataacc atgctactgt
    38461 gcaatcttcc gcgccggttt ctgctgagcc actgctgcag gtgagcggcg cactcatcgc
    38521 cattattgcc ctgatcctcg ctgctgcctg gctggtaaaa cggctgggat ttgcccctaa
    38581 acgcactggc gttaacggtc tgaaaattag cgccagtgct tcgctgggcg cgcgtgaacg
    38641 ggtcgtggtg gtcgatgtgg aagatgcacg gctggtgctc ggcgttaccg cagggcaaat
    38701 caatctgctg cataaacttc ccccttcagc accaacggaa gagataccgc agaccgattt
    38761 tcagtcggtc atgaaaaatt tgcttaagcg tggcgggaga tcctgatgcg tcgtttgttt
    38821 tctgtcgcac cggtccttct ctggctgatt acgcccctcg ccttcgcgca actgccgggt
    38881 atcaccagcc agccgctacc tggcgggtat caccagccag ccgctacctg gcggtggaca
    38941 aagctggtcg ctcccggtgc agacgctggt gttcatcacc tcgttgacgt tcattccagc
    39001 gattttactg atgatgacca gtttcacccg catcattatt gtttttggtt tattgcgtaa
    39061 cgcgctggga acgccctccg cgccacctaa ccaggtattg ctggggctgg cactgttttt
    39121 gacctttttt attatgtcgc cagtgatcga caaaatttat gtcgatgcgt accagccatt
    39181 cagcgaagag aaaatctcaa tgcaggaggc gctggaaaaa ggggcgcagc cgctgcgtga
    39241 gtttatgctg cgtcagaccc gcgaggcaga tttagggttg tttgccagac tggcgaatac
    39301 cggcccgttg caggggcctg aagctgtgcc gatgcgcatt ttgctcccgg cctacgtgac
    39361 cagcgagttg aaaaccgcat ttcagatagg cttcacgatt ttcatccctt ttttgattat
    39421 cgacctggtg attgccagcg tgttgatggc attggggatg atgatggttc cccccgccac
    39481 cattgctctg ccctttaaac tgatgctgtt tgtactggtg gatggctggc aattgctggt
    39541 cggttcgctg gcgcagagct tttacagcta gagaggcaaa atgacacctg aatcggtcat
    39601 gatgatggga actgaagcga tgaaagtcgc gctggcactg gcagccccgc tgttgctggt
    39661 agcgctggtc acgggcctta tcatcagtat tttgcaggca gccacgcaga ttaacgaaat
    39721 gacgctgtcg tttattccga aaatcatcgc cgtatttatc gccatcatta ttgccggacc
    39781 gtggatgctc aatctgttgc tggattacgt ccgcaccttg ttcactaacc tgccgtatat
    39841 catcgggtag cccgtacaat gatgcaggag acaagcgatc aatggctttc ctggttaagc
    39901 ctgtacttct ggccgttact gcgcgtgctg gcgctcattt ctaccgcgcc aattctgagc
    39961 gaacgcagcg tgccgaaacg ggtaaaactg ggtctggcaa tgatgatcac gttcgccatt
    40021 gccccatcat tacctgccaa cgatgttcct gttttttcgt tctttgctct gtggctggcc
    40081 gtacagcaga tcctgatcgg cattgcgctt ggttttacca tgcaatttgc ctttgccgct
    40141 gtgcgaaccg ctggcgaaat tatcggtctg caaatggggc tgtcatttgc gacatttgtc
    40201 gatcctggca gccatctcaa tatgcccgtt ttagcgcgca tcatggatat gctggcatta
    40261 ctgctgttcc tgacatttaa cggtcattta tggttgattt cactgctggt cgataccttt
    40321 cacaccctgc cgattggtag cgaaccgttg aacagcaatg cgtttctggc acccaccaaa
    40381 gcagggagtt tgattttcct taacgggctg atgctggcgt taccgctcat tactctgttg
    40441 ctgaccctga atctggcatt aggtttactg aatcgtatgg ccccgcaatt atccattttt
    40501 gttattggat ttccattaac tctgactgtc ggcatctctt taatggcggc attaatgccg
    40561 ttaattgcgc ctttttgcga acatttattc agtgaaattt ttaatttgct ggctgatatt
    40621 attagtgaat taccattaat ataattccgt aacgtttatc atgctaccct aaggattatc
    40681 cgaaaaataa tatctgcgaa catcttccag gatactcctg tagcgaaata tttgttttaa
    40741 gctcactcac atatcgcaac atttacttta agacaattcc aggcaaatta tacaacactt
    40801 tacgggatag taagtccgcc tgaaaaattg cgagagtggc gcattaggtg acccatgttg
    40861 ttccgtttag tcatgatgaa atattcaggt aaggggaatt atcgttacgc attgagtgag
    40921 ggtatgccat gtcaacgatt attatggatt tatgtagtta cacccgacta ggtttaaccg
    40981 ggtatctgtt gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa accgttgatg
    41041 accttgccat agcttgtgat tcacagcgtc cttcagtggt gtttattaat gaggactgtt
    41101 tcatccacga tgcttctaac agtcagcgca tcaagcacat cattaatcaa catcccaata
    41161 cgttatttat cgtttttatg gcaattgcca atgttcattt tgatgaatat ctattggtca
    41221 gaaaaaattt attgatcagt tctaaatcga ttaaaccgga atctctcgac gatatccttg
    41281 gcgatattct gaaaaaagag acaacgataa cctcgttttt aaatatgccg acgttatcat
    41341 tgagccgaac cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga accattcaaa
    41401 tctccgacca aatgaatatc aaagccaaga ccgtttcatc gcataaaggt aatattaaac
    41461 gtaagatcaa aacgcataat aaacaggtta tctaccatgt cgtccgactg acggataatg
    41521 tgactaatgg tatttttgtc aacatgcgct aacacattct gactggtggt ttcccaccag
    41581 tcaggctgaa taagattact ctgctttctc aacaaagata ccgtcctgat gccctgcttc
    41641 attaaagaac cagatgccga gcgggtagtc ttccagcgca accaggtaca ttgtgccttc
    41701 actaaactcc tcaactgcca gtaccactcc aggacgacgc ggaccgccat ccgttttgac
    41761 tgttacccga tcattcacct tcatcgtttt cctcctgtgg ctttgtgcca gtgtagaaca
    41821 atttcgtttt tctggcagcg ccaagcgcgc gcgagtgctg attttctcga cggtctatac
    41881 ttaagagatg ccaggcggac ttaacgactg gcggcaacaa cagagtaacg gttgcgagga
    41941 aagatgatga aaaccgcaaa agtgtacagc gataccgcaa aacgtgaggt cagcgtcgat
    42001 gtcgatgccc tgctggcggc gatcaatgaa attagcgaaa gcgaagttca tcgcagccag
    42061 aacgattctg aacacgttag cgtcgatgga cgtgaatatc atacatggcg tgaattggcg
    42121 gatgccttcg aactggatat tcatgacttc agcgtctctg aagtgaatcg ttgaatgcac
    42181 aataaaaaaa tcccgaccct gagggtgtcg ggatgaaact tgcttaagca agaagcactt
    42241 aaaaaattcg ttacaccagg aaatctgatg tgttcatcac cttatctgca atttttttcg
    42301 ctgacaagaa aatattcgcg ctatgaatga ttaacgctac ttatgaatag aaatgtgacg
    42361 cgcgtcaccc cttggtggct ctggtgtcaa cgggatcgct cccgaaaaaa tcatctttga
    42421 ttatttgtgc tgttttgaga atcatcttgg taaggggagt actgcgcgac catgttttca
    42481 attcaacaac cactactggt ttttagcgat cttgatggca ccctgctgga cagtcatagt
    42541 tatgactggc aaccggcagc cccctggctc agccgtttac acgaagccaa tattcccgtc
    42601 attctctgta gcagtaaaac tttagcggaa atgctgtact tgcaaaaaat gttggggcta
    42661 caaggcttac cgctgattgc agagaatggc gcagtgatcc agcttgctga acaatggcag
    42721 gatatagacg gttttccacg catcatctca ggtattagcc atggcgaaat ctgccaggtt
    42781 ttaaatacgc tacgcgagaa agaacatttt aaattcacga cttttgatga tgtcgacgat
    42841 gcaaccattg ccgaatggac gggattaagc cgtagctagg cggcgctgac gcagctgcat
    42901 gaggcgtcgg taacgctaat ctggcgcgac agtgacgagc gtatggcaca atttatcgct
    42961 cgtctgaacg aactgggctt gcagtttatg cagggtgcgc gcttctggca cgtactggat
    43021 gcctctgccg gaaaagatca ggctgccaac tggattatcg cgacctatca acaattgtca
    43081 ggcagacgcc caaccacact tggcctgggc gatgggccaa acgatgcgcc tttactggag
    43141 gtaatggatt acgcggtgat tgtgaaaggg ctaaaccgtg aaggggtaca tctgcatgat
    43201 gaggatccgg cccgcgtctg gcgaacgcag cgtgaaggac cggaaggctg gcgtgaaggg
    43261 ctggaccatt ttttctccgc ccgttaagcg ttatcgctcg cgaatacccg attgcgcccg
    43321 gcctgttttg cgaggtaaag ccgacggtcg gccaatgact ggagttgttc aaaatcataa
    43381 tcaccggttt cctcgctgct acttaccccc agcgaggcac tgatgcgtat cgttgtactc
    43441 ttggcgatca gcatctcttt ttcatttaac ttcaggcgaa tacgttctgc gacctccgca
    43501 gcctgcgtca gattcgcgcc tggcagaatc acacaaaact cctcaccacc gacccgcccg
    43561 gcaacgtcct gcgcacgcaa ggaactgcta attaatccgg cagcatgaga aagaacacgg
    43621 tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa aatggtcaag atcgacctga
    43681 atgacagaaa aaggatgttg gtgcgtctga cacaatttag cgagcggacg ggctttttcg
    43741 aacagtgcgc cacggttata taaacgcgtt aaggtgtcgt gccacgcctg ccactgcaac
    43801 gagctttgca gaacatacat gttgctgacc atccggcgaa tcacatacca ggagaggagt
    43861 aacatggtgg taaagagcgc ccacagcagg gttaatgcaa tgctgatact gccgaaatcg
    43921 ccgcgcacgc cttcgcttag cgtatggacg cgcgccagca caccgtcgaa atggtccaga
    43981 cgttcccagc taacatagcg actgtccata cgaatgccgc cccgcgtgtc atgttccatt
    44041 gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat tccctgttgg atggtcagga
    44101 ttggaagagg tcaaaaatct cagcttactg tcatagagct gatactcacc atcgaggttt
    44161 ttatcgatgg cgtttcttaa aaattgctgc atagtacgca cgggaatact catccccagc
    44221 acgccatacc agtagttatt actgtctacc ggaacactga cggtaacctg cggttcagta
    44281 ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg attttctcgt
    44341 tgcgaatggc cgataaacca gggttgggtg acatagccgt aataacgcgt tggtacattg
    44401 cgcgtaaaca aggtcggctg cgtcgaaacg taaaatccag cacgcgagac atacatcgcc
    44461 tgttcaacca tcgacgagga gttgtgcgcc agtcgcagca agtaaccaac ttccagtgca
    44521 gcggtaattt cattgtcgag gctttcattt tcgcgagaca ggaaattccc ctcgctgact
    44581 aatgcatccg atacgccatt gactggcagg gtgcgccgtc ggttgagttc gatttgccag
    44641 gcgtgctcgt cgcgatgctg ttcgaactcg gttacggcat tacgcagaga ggtgaaatcc
    44701 agtggcgcca cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt gtcgacgtta
    44761 tactgcaaat gcttatcgag cgcgttggct acgttttcca gatgattacg ctagctggag
    44821 atataggcat cttccagcac caccacttca cgccaggtga gcaaggtgga aaaaagcaat
    44881 accacgataa agcagagatt aacgacatga ccgggcccca ggcggcgtgc gagttttttc
    44941 aaccagctct ggttttccat ttttgtctcg tgctgcaccc tgacgactcc caacctgctt
    45001 ttttatgatt ctggcataac ccggcaatag cgtaccgcaa aacagcgata attttataat
    45061 ggtggcatga taagacgcga ctatcattca ttgcaggcat tgggacacca ggccggatgt
    45121 gatccggcct ggtgttaatg ataccctttc aaacttcttc cgcagaacga ctcccctccc
    45181 ccggaacaag atcggtttca tgaaaagctt cccgcttgac cccgtaaccg tcataccaac
    45241 gacactcaac cataccgctg gagtatccag tgacaatcat ccgcgggccg ccctctttaa
    45301 ccgtaacttc ctcactaacc ataaagctca tactcgcctc cttttttcga gtgaaacttg
    45361 ttcaccttag ttgaagatgg cgaattttgc ataatagcca gtgcgagtat taatgtgcct
    45421 gaccgcgcat tttcgctact gctttcactc ccagcaccac gatgccgccg atgataaatc
    45481 caagaatcag atttaaaaca gtcggtaata tcatcgccac cactgcactt tgctgcccgg
    45541 cgatatgttc aatagcgtga tgcagcggcg caataccatg taccacaatc ccgccgccga
    45601 cgaggaacat cgccagcgtg ccgacaatcg ataatgcttt catcagccaa ggcgcgataa
    45661 tcaataaccc tttacctaat gcctgcatca gcgcgctaga tttttccgcc agccaatacc
    45721 ctaggtcatc aatcttaacg ataaccccta ccagaccgta gacgcccacg gtcactacca
    45781 gcgcgatgcc tgaaagcacc agcacctgat taagcaacgg cgcctctgcc acaatcccca
    45841 gcgtgatggc gacgatttcc gcagacaaaa taaaatcggt acgaatcgcc cctttaattt
    45901 tgtccttttc aaacttcagc ggatcctgcg ccgccagctt ctccagacgc tgctggctct
    45961 gcgctggatc ttctttatgt ttacgcgcct ccagcatatg cagcactttc tctacccctt
    46021 caaagcagag aaacgcgcca ccaatcatca acagtggcgt aatcgcccac gggataaacg
    46081 cactgatgat cagcgccagc ggcaccagaa tcactttatt gatcagcgat cctttcgcca
    46141 cgccccagac cacgggaagt tcccggttgg cccgcacgcc tgaaacttgt tgcgcattga
    46201 gcgataaatc atcccctaat acaccggcgg ttttcttcgc cgccagtttg cccatcacag
    46261 agatatcgtc cagcagtgtg gcgatatcat cgagcaacgt cagtaaacta ctcccggcca
    46321 aaagcattcc ctctcatttt tattgttgaa gggtgtaagt atgaagcaaa aacggcagct
    46381 ctgatacttg cttcacaggt caacatttat ttaacaatca agtgcaatta tatctctcgc
    46441 cagcagaatg attttcacgt ttactatgaa ccaccttttt atttccgccg taagggatta
    46501 tgcgtttccg ccagttgtta ccgctttttg gcgcgctgtt tgcgttgtat atcatttggg
    46561 gctcaaccta ttttgtcatt cggattggcg tgggaagctg gcctccgtta atgatggcgg
    46621 gcgttcgatt ccaggcagcc ggtattttat tgctggcatt tttgctactg cgcggacaca
    46681 aacttccccc gctacgtccg ctgctcaatg ccgcgctgat tggcctgtta ttgctggctg
    46741 tcggtaatgg catggtgacg gttgccgaac atcaaaatgt tccttccggc atcgccgccg
    46801 tagtgattgc aaccgtgccc ctctttaccc tgtgcttcag tcgcctgttt ggcattaaaa
    46861 cgcgcaaact ggaatgggtg ggtattgcca ttgggcttgc cggaatcatc atgctcaata
    46921 gcggtggaaa tttaagcggc aatccgtggg gcgcgattct gattttaatc ggctcgatta
    46981 gctgggcgtt tggctcagtt tatggctcgc gcattacctt acctgtaggg atgatggcgg
    47041 gtgcgattga gatgctggcg gcaggcgtgg tgttaatgat cgcatcgatg attgcgggtg
    47101 aaaaactgac ggcgctccct tccctgtcag gcttccttgc ggtcggctat ctggcgctgt
    47161 ttggttcgat tatcgccatt aacgcttata tgtatttaat ccgtaatgtc agtccggctc
    47221 tcgccaccag ctacgcctac gttaacccgg tggtcgcggt cttgctgggt acgggactgg
    47281 gtggcgaaac gctgtcgaag attgaatggc tggcgctcgg cgtaattgtt ttcgcggtgg
    47341 tactggtcac gttgggaaaa tatctcttcc cggccaaacc cgttgtcgcg ccagttattc
    47401 aggatgcatc aagcgagtaa atgaatcccc tgcgtgtcga tctgcgcgct ggcaccttcg
    47461 ccgcagatcc actcttccag acgctcggta agcgccgcat ccgtcagctt ctcgcgccta
    47521 cgtaacgcac actcccagac aatcagtacg cgccagccga gctcctgcaa gcgactgata
    47581 tcgcggcgat cgcgttcaac atttttacct atcttctcca gccagaactc agttcgagtc
    47641 gcaggcactt taaacagata gcagtgatga tgatgccaga agcagccgtg ggtaaatatc
    47701 acgcagcgat attcatcaac gacaaaatcc gggcgtccgg gcagactggc gtcctgaacg
    47761 cgaaacgcaa ggccctgccc ggttaacaaa ctggcgaggc gcttctcaat cgccgtatca
    47821 cgcgtggcaa tcgcgcgcat atttttgctg cgggtggcct tatcgtgaac gtcgaccatg
    47881 ttgtgcctct tgctgacgca acgccaccgc ctgtttgatt tttggctcaa gcagttttgc
    47941 caccgcggca aagaccggca cgaccaccga gttgccgaac tggcgatagg cctgagtgtc
    48001 cgaaaccgga atgcggaatt tcgcttctcc cggcgcttca aaacccatta agcgcgcgca
    48061 ttcacgaggc gttaaccgac gtggacgatg ttgctgattc agcggatcgt caaagtcttt
    48121 ctcacccgtg gccatatccc aaccgcgatc gattaaaatt tccgcgccat ctttgtaata
    48181 acgagcagac agcgtacggg tgacgctttg cggattgttc ggataaacca ttccataacc
    48241 gaagccgtta ccgcgcgcct gatgtttttt cgcatatcga tagaggtact tccacagcac
    48301 cggcgtcagg atatatttcg cctcaaccat cgggtccagc agctgcgcca gcgtcactcg
    48361 ctgtgcaggg aaacattcgc tgatatcacg cagagtaaaa tcggctttca gattcagatc
    48421 gcgacgaaaa cccaccagca cgatgcgttc acggtgctgc ggcaggaaat gtttaccatc
    48481 gatgattttc ggatcgtccg gcccgttatc ttctgcatcg gcaacgtcat agcccagttc
    48541 gtccagcgtc tgcatgatga tgcggaacgt ttttccctta tcgtgactct tcaggttttt
    48601 gacgttttcg agcacaaaca tcgccggacg acgcgcgtcg ataatgcgca ccacatcaaa
    48661 gaacagcgtg ccctgggtat cgcaggcaaa accgtgcgcc cgcccgagcg agttcttttt
    48721 cgatacgcca gccagcgaaa atggctgaca agggaaaccg gccagtaaaa catcgtgttc
    48781 aggaatgtgt tgacgaatat gttccgccgc cgcctcatca ctcacgcctt cttgatgact
    48841 gagggtgatg tcgcggatat cttcattaaa atgatgcgtc gccgggtcgc aataatggtt
    48901 ggctttataa gtgcgtaccg catgtttgtt ccattcgctg gtaaacacgc actgtccacc
    48961 aatcgattca aaaccacgac ggatgccgcc aattccggca aatagatcga taaagcgaaa
    49021 cgcataatgc ggatgatgtt ccggtggttt gggtaacaac gtttgcagat gggcaaactc
    49081 tttctcactt aaacggtgcc atgccgagtc attcgccagc gcacgtttta aaattgccgc
    49141 gctccagtga ttctcaccca caccattaag ctgcgccacc agcattttaa catcataaat
    49201 ttgcagcagt ttctccagca ttgcctgtgc ggcattcccg gtgctgtatg aatcggttac
    49261 tgatatattt tcctgcatag atttaaccgg ccatctgaga tggccgacag gttaacataa
    49321 ttactgccgt ttagggccac gcgctcagcc agcgggtgaa aacgcatcta tcaccttgtg
    49381 atcgatacct tcattctccc ccgccagttc ggcaccgagc ttcgccatca aacagcgcca
    49441 agctcaatgc ccctgaaacg gcaaatacgc gcgccaggcc aatcgccccc agggcttcca
    49501 gccgatccgc atcctggacg attttagctt ccgttgttaa gggggcgatt tgcgcgctga
    49561 aactgtgagc ggcaatggca tgacaaacgg cctcaatttt ctccgccgga aattgcacga
    49621 actcttcacg gagcaggcga cgcgtctctt ctgccgccag gattgaagaa cgctgccgtt
    49681 gcggatgatt tttcgccagg ctcacgatat cgtgaaaata acaggcggtt aaaatcacca
    49741 gcatatcgac atcgtcatct gccgccagtt tctgtgccgt agcccagaca cggcgaaaat
    49801 gacacaggtc atgcgccgcg tcctgatgtt gatgatggtt tttcaaccag ttctcgaact
    49861 atgcctgcca gtgttgtaac tccatacgcg ctccgttgcc aaagagtctc aaccttagca
    49921 gtttttattc atagcgtctg caacgtgcgg aacgaggtcg ggtgtaccat gcaatgccgc
    49981 ctaacaaggc accaaccagg gccataaaca gaaatccgac caatgctcct aaccacttcc
    50041 ccgcggtaga tcctaaatca ctttcaaatc ccgacacggg ggcatcgggg atccggctca
    50101 tcacccagat gtatcgcgcc attgcccaga cgaaatagcc acaccatgta tagaaagccc
    50161 ac
//
LOCUS       DAZXUL010000014        49670 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZXUL010000014 DAZXUL010000000
VERSION     DAZXUL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49670
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.014"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..116
                     /locus_tag="ACH3L1_000765"
     CDS             <1..116
                     /locus_tag="ACH3L1_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFT4075626.1"
                     /translation="NLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(110..1288)
                     /gene="setB"
                     /locus_tag="ACH3L1_000766"
     CDS             complement(110..1288)
                     /gene="setB"
                     /locus_tag="ACH3L1_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFT4075627.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVMTPTY
                     "
     gene            1656..2786
                     /gene="fruB"
                     /locus_tag="ACH3L1_000767"
     CDS             1656..2786
                     /gene="fruB"
                     /locus_tag="ACH3L1_000767"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFT4075628.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2786..3724
                     /gene="fruK"
                     /locus_tag="ACH3L1_000768"
     CDS             2786..3724
                     /gene="fruK"
                     /locus_tag="ACH3L1_000768"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFT4075629.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3741..5432
                     /gene="fruA"
                     /locus_tag="ACH3L1_000769"
     CDS             3741..5432
                     /gene="fruA"
                     /locus_tag="ACH3L1_000769"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFT4075630.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5856..6797
                     /locus_tag="ACH3L1_000770"
     CDS             5856..6797
                     /locus_tag="ACH3L1_000770"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFT4075631.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6785..7723
                     /gene="psuG"
                     /locus_tag="ACH3L1_000771"
     CDS             6785..7723
                     /gene="psuG"
                     /locus_tag="ACH3L1_000771"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFT4075632.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7817..9067
                     /locus_tag="ACH3L1_000772"
     CDS             7817..9067
                     /locus_tag="ACH3L1_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFT4075633.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9138..9832)
                     /locus_tag="ACH3L1_000773"
                     /pseudo
     CDS             complement(9138..9832)
                     /locus_tag="ACH3L1_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9963..10904
                     /gene="rihB"
                     /locus_tag="ACH3L1_000774"
     CDS             9963..10904
                     /gene="rihB"
                     /locus_tag="ACH3L1_000774"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFT4075634.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            11004..12254
                     /locus_tag="ACH3L1_000775"
     CDS             11004..12254
                     /locus_tag="ACH3L1_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFT4075635.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12310..13398)
                     /locus_tag="ACH3L1_000776"
     CDS             complement(12310..13398)
                     /locus_tag="ACH3L1_000776"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFT4075636.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13401..14258)
                     /gene="nfo"
                     /locus_tag="ACH3L1_000777"
     CDS             complement(13401..14258)
                     /gene="nfo"
                     /locus_tag="ACH3L1_000777"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFT4075637.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14332..15381)
                     /locus_tag="ACH3L1_000778"
     CDS             complement(14332..15381)
                     /locus_tag="ACH3L1_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFT4075638.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15480..16361
                     /gene="yieE"
                     /locus_tag="ACH3L1_000779"
     CDS             15480..16361
                     /gene="yieE"
                     /locus_tag="ACH3L1_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFT4075639.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16567..18036
                     /gene="lysP"
                     /locus_tag="ACH3L1_000780"
     CDS             16567..18036
                     /gene="lysP"
                     /locus_tag="ACH3L1_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFT4075640.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18328..20319
                     /gene="cirA"
                     /locus_tag="ACH3L1_000781"
                     /pseudo
     CDS             18328..20319
                     /gene="cirA"
                     /locus_tag="ACH3L1_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20351..21186)
                     /gene="yeiG"
                     /locus_tag="ACH3L1_000782"
                     /pseudo
     CDS             complement(20351..21186)
                     /gene="yeiG"
                     /locus_tag="ACH3L1_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21444..22112
                     /gene="folE"
                     /locus_tag="ACH3L1_000783"
     CDS             21444..22112
                     /gene="folE"
                     /locus_tag="ACH3L1_000783"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFT4075641.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22129..23205
                     /gene="yeiB"
                     /locus_tag="ACH3L1_000784"
     CDS             22129..23205
                     /gene="yeiB"
                     /locus_tag="ACH3L1_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFT4075642.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23347..24387
                     /gene="galS"
                     /locus_tag="ACH3L1_000785"
     CDS             23347..24387
                     /gene="galS"
                     /locus_tag="ACH3L1_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFT4075643.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24667..25665
                     /gene="mglB"
                     /locus_tag="ACH3L1_000786"
     CDS             24667..25665
                     /gene="mglB"
                     /locus_tag="ACH3L1_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFT4075644.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25726..27246
                     /gene="mglA"
                     /locus_tag="ACH3L1_000787"
     CDS             25726..27246
                     /gene="mglA"
                     /locus_tag="ACH3L1_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFT4075645.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27262..28272
                     /gene="mglC"
                     /locus_tag="ACH3L1_000788"
     CDS             27262..28272
                     /gene="mglC"
                     /locus_tag="ACH3L1_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFT4075646.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28308..29521)
                     /gene="preA"
                     /locus_tag="ACH3L1_000789"
                     /pseudo
     CDS             complement(28308..29521)
                     /gene="preA"
                     /locus_tag="ACH3L1_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29515..30753)
                     /gene="preT"
                     /locus_tag="ACH3L1_000790"
     CDS             complement(29515..30753)
                     /gene="preT"
                     /locus_tag="ACH3L1_000790"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFT4075647.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30947..31186)
                     /locus_tag="ACH3L1_000791"
     CDS             complement(30947..31186)
                     /locus_tag="ACH3L1_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFT4075648.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31189..31908)
                     /gene="sanA"
                     /locus_tag="ACH3L1_000792"
     CDS             complement(31189..31908)
                     /gene="sanA"
                     /locus_tag="ACH3L1_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFT4075649.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32058..32942)
                     /gene="cdd"
                     /locus_tag="ACH3L1_000793"
     CDS             complement(32058..32942)
                     /gene="cdd"
                     /locus_tag="ACH3L1_000793"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFT4075650.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33072..33767)
                     /locus_tag="ACH3L1_000794"
     CDS             complement(33072..33767)
                     /locus_tag="ACH3L1_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFT4075651.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33764..34162)
                     /locus_tag="ACH3L1_000795"
     CDS             complement(33764..34162)
                     /locus_tag="ACH3L1_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFT4075652.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34401..35348
                     /gene="dusC"
                     /locus_tag="ACH3L1_000796"
     CDS             34401..35348
                     /gene="dusC"
                     /locus_tag="ACH3L1_000796"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFT4075653.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35719..35802)
                     /gene="yohP"
                     /locus_tag="ACH3L1_000797"
     CDS             complement(35719..35802)
                     /gene="yohP"
                     /locus_tag="ACH3L1_000797"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFT4075654.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            36026..37462
                     /gene="mdtQ"
                     /locus_tag="ACH3L1_000798"
                     /pseudo
     CDS             36026..37462
                     /gene="mdtQ"
                     /locus_tag="ACH3L1_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37530..38276
                     /locus_tag="ACH3L1_000799"
                     /pseudo
     CDS             37530..38276
                     /locus_tag="ACH3L1_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38393..38971)
                     /locus_tag="ACH3L1_000800"
     CDS             complement(38393..38971)
                     /locus_tag="ACH3L1_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFT4075655.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39141..39728
                     /locus_tag="ACH3L1_000801"
     CDS             39141..39728
                     /locus_tag="ACH3L1_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFT4075656.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39902..40834
                     /gene="pbpG"
                     /locus_tag="ACH3L1_000802"
     CDS             39902..40834
                     /gene="pbpG"
                     /locus_tag="ACH3L1_000802"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFT4075657.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40872..42587)
                     /gene="dld"
                     /locus_tag="ACH3L1_000803"
     CDS             complement(40872..42587)
                     /gene="dld"
                     /locus_tag="ACH3L1_000803"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFT4075658.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42783..45080
                     /gene="bglX"
                     /locus_tag="ACH3L1_000804"
     CDS             42783..45080
                     /gene="bglX"
                     /locus_tag="ACH3L1_000804"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFT4075659.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45332..46249
                     /gene="osmF"
                     /locus_tag="ACH3L1_000805"
     CDS             45332..46249
                     /gene="osmF"
                     /locus_tag="ACH3L1_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFT4075660.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46256..47413
                     /gene="yehY"
                     /locus_tag="ACH3L1_000806"
     CDS             46256..47413
                     /gene="yehY"
                     /locus_tag="ACH3L1_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFT4075661.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47406..48332
                     /gene="yehX"
                     /locus_tag="ACH3L1_000807"
     CDS             47406..48332
                     /gene="yehX"
                     /locus_tag="ACH3L1_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFT4075662.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48337..49068
                     /gene="yehW"
                     /locus_tag="ACH3L1_000808"
     CDS             48337..49068
                     /gene="yehW"
                     /locus_tag="ACH3L1_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFT4075663.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(49049..49156)
                     /locus_tag="ACH3L1_000809"
     CDS             complement(49049..49156)
                     /locus_tag="ACH3L1_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFT4075664.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49216..49670)
                     /locus_tag="ACH3L1_000810"
                     /pseudo
     CDS             complement(49216..49670)
                     /locus_tag="ACH3L1_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        12000 a        12234 c        13345 g        12091 t
ORIGIN      
        1 tgaatctgag acaacatctg gcaaggctgg gacggaagtc actgtcgttc tcaaaatcgg
       61 tggagctgca tgacaaggtc atcgggcatt atctgaacat aaaacactat cagtaagttg
      121 gagtcattac ccaaaacaga gtggcgataa tcatcaccat cgcaaaccag aacacagcgt
      181 gataattcca gatctcggcg acgatgcccg ccactgatcc tgcgatgatc cagcccacgc
      241 gcgacgtgtt ggtatagagc gtggtggctg aacctgcctg accgggcatc aaatcctgaa
      301 aatagagcat cccgatgccg cccagaatgc caataaaaat agcatttagc agctgcaagc
      361 ccaacagaat ggcaggtgaa tgcgccatca gcattcctgc gtaaaaacag acgccaccca
      421 cggcagcaac gcgcattaag aaacgcttac ccagacgttt ggcgaaatat ccggcaatca
      481 acatcgtcgg gatttccagc ccggcggcgg tccccatcat cacaccggcc agtttctcgg
      541 gaagatgcag ttcgttgata ataaatagcg gcatgttgat gatgtacagg ctgttcgagc
      601 cccacatcaa tgtacaaatg acaaacagca gtagcgtatc gcgacggtta cggcgcggcg
      661 cttcaaccgt gccggtcgcc agcggaagct ctttttgcat cgacggtaaa aacagccaca
      721 ccatcacgcc gcaaacaata aacgctaccg ctgcgctcag atacattacc gtaaagctga
      781 aacccatcgc taacgcataa gccagcggtg ggccaatgac ccatgccagt gaaacctgag
      841 cgcgtaaaaa agagctgaac atcaccgcct cacgtccggt tttgtcggca tgttcacggg
      901 caagggcaaa catttgcggg ttagcggtcg agccaaagct gctaagaaag acgccaacga
      961 atagcaaaac aaagtagttg cgattccaag caaaaagggt gcaggccagc acgcctaaca
     1021 ggcagcaaaa gacaatcagc gatttgcgat cgccgcgctt atcagagcgc ccagcgagaa
     1081 actgacttac cagaatccca atgacagcgc tgccggtaaa gaagaatccc accatcgccg
     1141 gacgggcatg tacttcatcg gtaaggaaaa tactgagtgt cggggtttgc agagcacccg
     1201 caataccggt gagaaaggca acgattaaaa acgccgtcga ggtcaggtca aacgatttcg
     1261 cgctggagac tgcgggggag ttatgcatgc tggtcgatca ctggaagagg aagacgcgga
     1321 gtttacgctg cttgtcagaa aataaacaga cagcgaaatc aaaagcgcca cgaataatca
     1381 aaatgccatt tcactgttgc tgattggctg ctgacgctgc tgaaatgaaa ttgctgataa
     1441 cggattttcc catcagcaat taggaaaaat gacaaaaaat tgtgcagcac atcaaacttt
     1501 tgctcataac tttacggctt tccttgcgtg ctgaaacaga agtattatgc tttcttgaaa
     1561 cgtttcagcg cgatcttgtc tttaacccta agctaggttg gcgctttttt tctcatagag
     1621 gctgaatcgt ttcaattcag caagagagga gaactatgtt ccagttatcc gtacaggaca
     1681 tccatccggg cgaaaaggcc ggagacaaag aagaggcgat tcgccaggtc gctgcggcgc
     1741 tggtgcaggc cggtaatgta gcagaaggct acgtcaatgg catgctggcg cgcgaacagc
     1801 aaacctcaac gttcctcggc aatggtattg ctattccaca cggcactacc gacacccgcg
     1861 atcaggtgct gaaaaccggc gttcaggtat ttcagttccc ggaaggcgtc acctggggtg
     1921 acggtcaggt agcgtacgtg gcaatcggta ttgctgccag ctcggataaa catctgggcc
     1981 tgctacgcca gctgacccac gtactgagcg atgattccgt tgctgaacaa ctgaagtcag
     2041 caacaacagc agaagaactt cgcgcattac tgatgggcga aaagcagagt gagcagctga
     2101 agctcgacaa cgaaatgctg acgctggata tcgtcgccag cgatctgctg actcttcagg
     2161 cgctgaacgc tgcgcgtctg aaagaggcga gggcagttga cgccactttc gtcaccaaag
     2221 ccatcaatga acaaccgctg aatctcggac agggtatctg gctgagcgat agcgccgaag
     2281 gcaatctgcg tagtgcgatt gcggtaagcc gtgcggcaaa tgcttttgat gtggacggcg
     2341 aaacggcagc catgctggtg agtgtggcga tgaatgacga tcagcccatc gcggttctta
     2401 agcgtctcgc tgatttgttg ctcgacaata aagctgaccg cttgctgaaa gcggatgcgg
     2461 caacgttgct ggcgctgctg accagcgatg atgcgccgac cgacgacgtg ttaagcgcgg
     2521 agtttgtggt gcgcaatgaa cacggcctgc atgctcgtcc aggtaccatg ctggtcaata
     2581 ccattaaaca atttaacagt gatattaccg tgacaaacct tgatggcacc ggcaaaccgg
     2641 caaacggacg tagtctgatg aaagttgtgg cacttggcgt taagaaaggt catcgcctac
     2701 gctttaccgc ccagggtgca gatgctgaac aggcgctgaa agccattggc gacgctatcg
     2761 ctgctggtct tggggagggc gcataatgag cagacgtgtt gctacgatca cccttaatcc
     2821 ggcttatgac cttgttggtt tctgcccgga aattgaacgc ggcgaagtga acctggtgaa
     2881 aaccaccggt ctgcatgcgg cgggtaaagg catcaacgtg gccaaagtat taaaagacct
     2941 gggaattgat gtcaccgttg gcggcttcct gggtaaagac aatcaggatg gttttcagca
     3001 actgttcagc gagctgggca ttgccaaccg tttccaggtt gtacaggggc gcacccgaat
     3061 taacgttaag ctgacggaaa aagacggcga agtgaccgac ttcaacttct cgggtttcga
     3121 agtcaccccc gccgactggg aacgctttgt gactgattct ctgagctggc tcggtcagtt
     3181 cgatatggtc tgtgtcagcg gaagcttacc gtcaggcgtc agcccggaag cgttcaccga
     3241 ctggatgact cgcctgcgta gtcagtgtcc ttgcattatc tttgatagta gccgtgaagc
     3301 gttagtagca ggtttgaaag cggcaccgtg gctggtgaaa cctaaccgcc gcgagttgga
     3361 aatctgggca ggccgtaaac tgcctgaaat gaaagatgtg attgaagctg cacatgcgct
     3421 acgtgaacaa ggtattgcgc atgttgttat ttcactgggt gccgaaggcg cgctttgggt
     3481 taatgcctcc ggcgaatgga tcgctaaacc accgtcagtc gatgtcgtaa gcaccgttgg
     3541 cgcaggggat tctatggttg gtggcctgat ttatggcttg ctgatgcgtg aatccagtga
     3601 acacacactg cgtctggcga cagctgttgc agccctggcg gtaagtcaaa gcaatgtggg
     3661 tattaccgat cgtccgcagt tggccgcaat gatggcgcgc gtcgacttac aaccttttaa
     3721 ctgacagcag gagaggcata atgaaaacgc tgctgattat tgacgctaat ctcggtcagg
     3781 cacgcgccta tatggcgaag accctgctgg gcgcggcggc gcgaaaagca aaactggaaa
     3841 tcatcgacaa tccgaacgac gcagaaatgg cgattgttct cggtgattcc atcccgaatg
     3901 acagcgcgct gaacggtaaa aatgtctggc tgggtgatat ttcccgggca gttgcgcacc
     3961 ctgagctgtt cctgagtgaa gccaaaggcc atgcgaaacc ttacactgcg ccggtcactg
     4021 cgacagcacc ggttgccgcc agcggtccga aacgcgtagt tgcggtgact gcttgcccga
     4081 ctggcgtagc acacaccttt atggcggctg aagccattga aaccgaagcg aaaaaacgtg
     4141 gctggtgggt gaaagttgaa acccgtggtt ctgttggcgc gggtaatgca atcactcccg
     4201 aggaagtagc cgcagcggat ctggtgattg tggcggcaga tatcgaagtg gatctggcga
     4261 aatttgctgg taaaccgatg tatcgtacct ctaccggtct ggcgctgaag aaaacctcgc
     4321 aggaactgga taaagcggtt gctgaagcaa cgccgtatga accggcgggc aaagctcaaa
     4381 cggcgaccac tgaaggtaag aaagagagtg caggcgctta tcgtcacttg ctaacgggcg
     4441 tctcttacat gctgccgatg gtcgttgctg gtggtctgtg tatcgcgctt tcttttgctt
     4501 ttggtatcga agcgtttaaa gagccgggta cgttggcagc ggcgctgatg cagattggtg
     4561 gtggttcagc ctttgcgctg atggtgccgg tactggcagg ttatattgcc ttctccattg
     4621 ccgatcgtcc gggcctcacg ccgggtctga ttggcggtat gctggcggtc agcaccggtt
     4681 ctggcttcat tggcggtatt attgcgggct tcctggctgg ttacattgcg aagttaatca
     4741 gtacgcaact gaaactgccg cagagtatgg aggcgctgaa accgatcctg atcattccgc
     4801 taatttccag tctggtggtc ggtctggcga tgatctacct gatcggtaaa ccagttgctg
     4861 gcattctcga agggctgact cactggctgc agaccatggg gactgcgaat gcggttctgc
     4921 tgggggcgat cctcggtggc atgatgtgta ctgacatggg cggtccggta aacaaagcag
     4981 cgtacgcatt cggtgtgggt ctgctgagta ctcaaaccta tggcccgatg gcggcgatta
     5041 tggcggcagg tatggtgcca ccgctggcaa tgggcctggc aacaatggtg gcgcgtcgca
     5101 aattcgacaa agcgcagcag gaaggtggca aagccgctct ggtattggga ctgtgcttca
     5161 tttcggaagg tgcaattccg tttgctgctc gtgatccgat gcgtgtgctg ccgtgctgta
     5221 tcgtgggtgg ggcgctgact ggcgcaatct caatggcgat tggtgcgaaa ctgatggcac
     5281 cgcacggtgg tctgtttgtt ctgctgatcc ctggcgctat tacgccggta ttgggttacc
     5341 tggtagcaat tattgccggt acgctggtgg cgggtttggc ctatgccttc ctgaaacgtc
     5401 cggaagtgga cgcagtagcg aaagcagcgt aataaaaggt gtgttacagg gcagaaattt
     5461 cctgccctgt aacatttgat gagcgcaaag agaatgacca gcttgtgaat gtaattttat
     5521 gaatttaaat ggttttatct ttcagtagaa acttcaaacg ttcatcaaac actaaggtta
     5581 tttcctcaag ggcagattat tttctgccct tttttagtgt tcctgttctg tgcatcccgt
     5641 cacaaattcc tcgcttttgt aattcaggca tttaatcgca ttttgtgagc ctgctcaaat
     5701 ctgcccattt tgcctctctg gcggtaattt actttacgta aaacatgcat actatgagca
     5761 catgtttaaa tatgaacaca tgtttaaacc gtctttgcag tatctatatt cttgtggatt
     5821 gacagggttg gtaatttttt accagaggac gtgctatgcg cgaaaaggat tatgtcgtaa
     5881 ttataggttc ggcgaatatt gatgtcgccg gatattcaca tgaatcatta aattatgcgg
     5941 attcaaatcc aggtaaaata aaatttacgc ctggtggagt agggcgcaat attgcacaaa
     6001 acctggcgtt gctgggtaac aaagcctggc tactgagcgc cgtaggcagt gatttttatg
     6061 gtcaatcgct gctaacgcaa accaatcaat ctggtgttta tgtcgataaa tgcctgattg
     6121 tgccgggaga aaatacgtcg agttatttat cattactcga taataccggt gaaatgctgg
     6181 ttgctataaa tgacatgaat attagcaacg ctattacagc tgaatatctc gcacagcacc
     6241 gtgaatttat tcagagggca aaggtcattg tcgcggactg taatatcagt gaagaggcac
     6301 tggcatggat tctggataat gccgccaacg tacccgtatt tgtcgatccg gtttcagcgt
     6361 ggaaatgtgt caaagtgcgc gaccgtctaa atcagaacca cactctgaag ccaaaccgcc
     6421 ttgaagcgga aaccctgagt gggattgcgc tgtcagggcg tgacgatgtg gcaaaagttg
     6481 ctgcctggtt ccatcaacat ggcctgaacc gactggtatt gagcatgggc ggcgacggcg
     6541 tttattacag cgatatcagc ggtgaaaatg gctggtctgc gccgatcaaa accaatgtta
     6601 ttaatgttac cggagcgggc gatgccatga tggcgggact tgcttcgtgt tgggtagacg
     6661 gaatgccgtt tgccgaatct gttcgtttcg cacagggatg ttcgtcaatg gcgctctcct
     6721 gtgaatacac caataacccc gatttatcga ttgccaacgt tatatcgtta gtggagaacg
     6781 cagaatgtct gaattaaaaa tttcccctga attattacaa atttctccgg aagtgcagga
     6841 cgctttaaaa aacaaaaaac cggttgtggc gctggaatcg accattattt ctcacgggat
     6901 gccgttccca caaaatgccc agaccgcaat tgaagtagaa gaaactattc gtaaacaggg
     6961 cgcagtacct gccactatcg ccattattgg cggcgtgatg aaagtgggtt taagcaaaga
     7021 aaaaattgaa ttactgggtc gtgaagggca taacgtgacc aaagttagtc gtcgcgattt
     7081 accttttgtt gttgccgccg gaaaaaatgg cgcaaccact gtggcttcaa cgatgattat
     7141 tgcggcgctt gccggaatta aagtatttgc caccggggga attggtggtg tgcatcgcgg
     7201 ggcggaacat accttcgata tttctgccga tttgcaagaa ctggcaaata ctaatgtcac
     7261 cgttgtttgt gccggggcga aatctattct cgatttagga ttaaccactg agtatttaga
     7321 aaccttcggt gtgccgttaa ttggctatca gactaaagcg ctgcctgcgt ttttctgccg
     7381 taccagctcg tttgacgtca gcattcgtct cgacagcgcc agtgaaattg cccgtgcaat
     7441 ggcggtgaaa tggcaaagcg ggctgaacgg tggcctcgtg gtagcgaacc cgatcccgga
     7501 acagtttgcg atgccggagg aatctatcaa tgcagccata gatcaagccg tcgccgaagc
     7561 cgaagagcag ggcgttattg gtaaagaaag tacaccgttc ctgctggcgc gcgttgctga
     7621 gctgaccagc ggtgacagcc tgaaatccaa tatccagctg gtgttcaaca acgccattct
     7681 ggcgagtgaa attgccaaag aataccagcg tctcgcgggt taatttctga cgggcagaaa
     7741 gccgtcgccc gtactcatca agcataccaa aacctggcgc tgacgccggg tttactggca
     7801 tgaagggaaa atcattatgg atataatgag aagtgttgtg gggatggtgg tgttactggc
     7861 aatagcattt ctgttgtcag tgaataaaaa gagcatcagt ttgcgcacgg ttggagccgc
     7921 actgctgctg caaatcgcta ttggtggcat catgctctac ttcccaccgg gaaaatgggc
     7981 agtagaacag gcggcattag gcgttcataa agtgatgtct tacagtgatg ccggtagcgc
     8041 cttcattttt ggttcgctgg ttgggccgaa aatggatgtc ctgtttgacg gtgcgggttt
     8101 tatcttcgcc tttcgcgtac ttccggcgat tattttcgtt actgcgctca tcagtctgct
     8161 gtactacatt ggcgtgatgg ggctgctgat tcgcatcctt ggcagcattt tccagaaagc
     8221 cctcaacatc agcaaaatcg aatcttttgt tgcggttacc actattttcc tcgggcaaaa
     8281 tgagatcccg gcgatcgtta aaccgtttat cgatcgcgtg aatcgcaacg agttgtttac
     8341 cgcaatttgt agcgggatgg cgtccattgc tggttcggtg atgattggtt atgccggaat
     8401 gggcgtacca attgactacc tgttagcggc atcgctgatg gcgatccccg gcggtatttt
     8461 gtttgcacgt attcttagcc cggcaaccga gccttcgcag gtcacatttg aaaatctgtc
     8521 gttcagcgaa acgccgccaa aaagctttat cgaagcggcg gcgagcggtg cgatgaccgg
     8581 gctgaaaatc gccgctggtg tagcgacggt ggtaatggcg tttgtcgcaa ttattgcgct
     8641 gatcaacggc attatcggcg gaattggcgg ctggtttggt ttcgccaatg cctctctgga
     8701 aagtattttt ggctatgtgc tggcaccgct ggcgtggatc atgggtgtgg actggagtga
     8761 tgccaacctt gctggtagcc tgattgggca gaaactggcg attaacgaat tcgtcgctta
     8821 cctgagtttc tccccatacc tgcaaacggg cggcacgctg gaagtgaaaa ccgtggcgat
     8881 tatatccttt gcgctttgtg gttttgctaa ctttggttct atcggtgttg tcgttggcgc
     8941 attttcggct atttcgccaa aacgtgctcc agaaatcgcc cagcttggtt tacgggcgct
     9001 ggcagcagca acgctttcca acctgatgag tgcgactatt gccgggttct ttattggtct
     9061 ggcgtaatgc tcgatttgta gaccggataa aacgtattaa gcgttgcatc cggcaatcgc
     9121 gctattgcaa ataattttta ctgcatcatc ccggagaatt tattctccgg gtccatctcc
     9181 agcgccagtc ctgacaactg ctttctgttt ttaattagat atcctttctt ttaattaata
     9241 aaccgtcgtg aatgaattgc gcgagaacat ataaaagatg tcgataagaa acgcctaaat
     9301 actctgccgc ttgcgtatgc ttttcgtgat aaagatcacc ttcttgtgag agtaatataa
     9361 atgctgccag gcgattcact aacggaaatg attgattctg agttaaagaa acaatattac
     9421 gataattttt atgacttaag gtgacgcaaa gttttcgtaa aaatagggtg tcgtttaata
     9481 acagcggacg gtaatgtttc atagggagcg caaggcacca acactcttca atagcctgca
     9541 ccgcacgcgg ttcatggtct ttgtcgatta actcaatctc gccaataaaa cagggagcgg
     9601 caaagaaatc gatcagcgac acgcgaccat tggcaagcgt ggcgtaaagc ctggcgcggc
     9661 ctcgcgtcag ataaaacagc catgacggct gttgcccttc ctgcacaatg tagtcacgcg
     9721 ctaaaaaatg aaacagccgc gtatcggctg aaacatccgt tgaaaagcaa tccttaaacg
     9781 cagattcact catcaattgc tgcttaaggt cattattgtg gatttctttc acgatgttta
     9841 tccgctatga gatttctcat atttagcata cgtacgctgt tgttaagatt caatcattgg
     9901 cccggtcgaa ttttctttgc tgtagaaaaa aataacgcaa ctggaaacag aggaaataaa
     9961 caatggaaaa gagaaaaatt attctggatt gtgagccggg tcatgatgat gctattgcaa
    10021 tgatgatggc agcgaaacat ccggcaatag atttattagg catcactatt gtagcgggta
    10081 atcagacgct tgataaaaca ttaattaatg gcctgaatgt ttgccagaaa ctggagatta
    10141 atgttccggt ttatgcgggg atgccgcaac ccattatgcg taaacaaatc gttgccgata
    10201 atattcacgg tgaaaccgga ctggatggcc cggtattcga gccgctgacc cgccaggcag
    10261 aaagtaccca tgcggtgaaa tatatcatcg ataccctgat ggcaagcgat ggcgatatca
    10321 ctctggtgcc ggttggtccg ctttcaaata tcgcggtggc aatgcgtatg caacccgcaa
    10381 tcctgcccaa aatccgtgaa attgtgctaa tgggcggcgc ttacggtaca ggcaacttca
    10441 ccccatctgc cgagttcaat atctttgccg acccggaagc cgcccgcgtg gtgttcacct
    10501 ccggcgttcc attagtgatg atgggcctcg atcttaccaa ccagaccgtt tgcaccccgg
    10561 atgtaattgc tcggatggaa agggtaggcg gccctgccgg agagctgttc agcgacatca
    10621 tgaacttcac tctcaaaacg cagttcgaat actacggcct ggctggcggc ccggtgcatg
    10681 acgccacctg cattggttat ctgatcaacc ctgatggcat taaaacccag gatatgtacg
    10741 tcgaagtgga cgtcaacagc ggcccttgct acgggcgtac ggtctgcgac gaactgggcg
    10801 ttcttggcaa gcccgctaat accaaagtcg gcatcactat tgataccgac tggttctggg
    10861 gcttagtcga agagtgcgtg cgcggctaca tcaaaaccca ttaagtgttg ataacaagcc
    10921 gggacagtat cgcaagatgc aggcccggct atttctgatg ggcagaaagc cgtcgcccaa
    10981 aatgacatga agagataatc actatggatg tcatgagaag tgttctggga atggtggtac
    11041 tgctggcaat tgcgttttta ctgtcagtaa acaagaagaa gatcagcctg cgtaccgttg
    11101 gcgcggcgtt agtgttacag gtcgtgattg gcgggattat gctttggtta ccgccagggc
    11161 gttgggtcgc agaaaaagtc gcttttggcg tccacaaagt gatggcgtac agcgacgcag
    11221 gtagcgcatt tatcttcggt tctctggtcg gaccgaaaat ggatacctta tttgatggcg
    11281 caggatttat ctttggtttc agggtattac cggcaattat cttcgtcact gcactggtga
    11341 gtattctcta ctacatcggt gtgatgggga tcttaattcg cattctcggc ggtatcttcc
    11401 agaaagcttt aaatatcagc aagatcgagt cattcgtcgc ggtcaccacc attttcctcg
    11461 ggcaaaacga aattccggcg atcgtcaaac cctttatcga tcgtctgaat cgcaatgaat
    11521 tatttacagc gatttgtagt ggcatggcct cgattgctgg ttcgacaatg attggttacg
    11581 ccgcactggg cgtacctgtg gaatatttgc tggcggcatc gttaatggcg atccctggcg
    11641 ggatcttgtt tgcccgcctg ttaagcccgg ctacggaatc ttcgcaggtt tcttttaata
    11701 acctctcttt caccgaaaca ccgccaaaaa gcattattga agccgatgcg acaggggcaa
    11761 tgaccgggct gaaaatcgcc gccggtgtag cgacagttgt tatggcattt gtcgccatca
    11821 ttgcgttaat taacggtatt atcggcggcg ttggcggctg gtttggtttt gaacatgcct
    11881 cgctggagtc cattttaggt tacctgctgg ccccacttgc gtgggtaatg ggtgttgact
    11941 ggagtgatgc aaatcttgcc gggagtttga ttggacagaa actggcaata aatgaatttg
    12001 tcgcttatct caatttctca ccctatctgc aaacggctgg cactctggat gctaaaaccg
    12061 tggcgattat ttccttcgcg ttgtgcggtt tcgctaactt tggttctatc ggggtggtgg
    12121 tgggggcgtt ttctgcggtt gcgccacacc gtgcgccgga aatcgcccag cttggtttac
    12181 gcgcgctggc ggcggcgaca ctttctaatc tgatgagtgc caccattgcc gggttcttta
    12241 ttggtttagc gtagtgtgca atttatgagc cggattagat gcggtcaagc gtcgaatccg
    12301 gcaacattgt tacaccattg gcactaatga cagcgcgtta tcggcagaca gggtagggtt
    12361 gtttaagctg ccgctggcgc gtgaaattgc cgcgcaggcc atcgcaaaac gggcgctgtc
    12421 acggaaattg tttccctcca gaaagctata taccagaccc gccataaaac catcgtcagc
    12481 accaaaactg tctaccgtcg tatgcgctgg cacagtcagc aaaaattgtt caccatcctt
    12541 ttcgctgcaa taaactgact catcgggcaa ataaacaaac agttgctgaa caccttgctg
    12601 atgcaatgca ttcactgcgg cattacggtc agcatcgctg gtgatcgcct gtccccataa
    12661 aatttccagc tccggtaaag tgggtttcag ggtgtgaata tgcgccagcc agtgtttgat
    12721 tttgcccgct ttgaattctg aaacggtatc gacaaacacc gggatctcat cagcgagggt
    12781 aaagacccat tccagcgcct cggctgtcag gttgcaatca gccagtacca cgcccgcatg
    12841 acgaagtaaa tcgcgcgacc cgttcagtag ttgcggtgtc aactgttcca gcagatgggt
    12901 atcgttaatt gccagcacgg tttgatcgtc tcgattggca attgccagat acgtcgatgt
    12961 gctttgacca tgcaaacgaa cgcagccgga gacattcacg ccggcacggc gcgtttcttc
    13021 caggagcatt tcgccataaa agtcatcgcc aatcactgaa agcaaatgga cgtcacggcc
    13081 taacagcgcc agattgtggg cgatattgcg tccaacgccg cctgccgaac aatgaattgt
    13141 accgggatga gaagccgctt gcgggtaatg gatatccgcc atcccgcgaa tatccatatt
    13201 gattgtcccc accactacgc agtattcctg ctcggtgaga atgtaacctt tgtctttaat
    13261 ccggcctttg cgcattaaat ccataatatg cgccgcaacg cgcgaacgac tgatttgcag
    13321 catgtccgca atttcgttct gctgaatcag cgggttacgc cgtaaaattg caaggatctc
    13381 cttttcccgg ttatttatct tcaggctacc gctttttcag tttgttgcgc tttcagccag
    13441 gcgatctctt ctgcccagat atccgggttg atggtttcaa ggatcagcgg aatgccgtcg
    13501 aaacggtcgt tctgcataat ccagcggaac gcatcatgac cgatattgcc ttcaccgagg
    13561 ctatgatggc ggtcaacgcg gctgccaaag gtacttttcg catcgttaag gtgcatcccg
    13621 cgcaaatact taaagccgac aatacgggca aaatcagcga acgttttttc gcattcggct
    13681 ggagtacgca aatcataccc ggcagcgaaa gcatggcagg tatcaatgca gacgccgacg
    13741 cgggatttat cttccacgcc gtcgataatc gccgcgagat gttcgaattt aaaccctaag
    13801 ttactgccct gaccggcggt gttttcaatc accgctgtta caccttgagt tttatccagc
    13861 gcaatgttga tggattcggc aatacgcgca aggcaatcct cttctgaaat ctgcatcaga
    13921 tggctgccag ggtggaagtt gagcaaagaa agccccagtt gttcgcaacg ctgcatttca
    13981 tctataaagg catcgcgcga tttttccaga gcttcagtga ccggatgtcc gaggttgatc
    14041 agataactgt cgtggggaag aatttgcgcc gatgtgtagt gatatttttc acaggcggct
    14101 ttgaattcat cgatggtttg cgtcgtgagc ggtgcggcac gccactgacg ttggtttttg
    14161 gtgaacaagg caaacgcggt tgcgtcgatt tcggcggcgc gaattgcggc atttgccaga
    14221 ccgccagcag cactaacgtg cgctccaatg tatttcatgc gaggactcct gttaaacccg
    14281 ctggaggaaa acggtaatga tagcgggtta acaggagcaa gaagtagtgg tttatgcgat
    14341 gacgctttga atcacatagt taatcgcacc accaccaaca atcagccagg caaacagtac
    14401 cagtgccatc agcagaggtt tcgccccagc ttttttcagc gcgctgacgt gagtggtcag
    14461 acccagcgcc gccatcgcca ttgccagcag gaaggtatcc agcgttacca gcatgttcac
    14521 cacgctctgc ggtaacaggt ggaacgagtt aaagatggca actacgatga acaagatggc
    14581 aaaccacgga atagtgattt tgcttttctc gccgctgttc gccccagaca gctgtttaac
    14641 acgcgccgcc agcaggatga ggaacggagc cagcatcatc acgcgcagca ttttggaaat
    14701 aactgctgcg ttttctgcat ccgggctgat ggcatgacct gccgccacca cctgcgccac
    14761 ttcgtgcaca gtagaaccaa tgtagatacc gaaagtttcc ggactaaacc attgagacat
    14821 cagcggatat atcgccgggt agaggaaaat cgcgacggtc ccgaagataa caacggttgc
    14881 aacagccacg gttactttac tggcttccgc tttcactacc ggctcagtcg ccagtaccgc
    14941 ggcagcacca cagatactgc taccggcacc gatcaaccaa ctggtgtgct tatccagacc
    15001 aaacactttc tgccccagga agcaagccag caggaaggta ctggacagcg tcagcacgtc
    15061 aatgatgatc ccactgatac cgacatcggc aatttgcgag aacgtcagac ggaagccata
    15121 aagaatgata cccagacgta ataaatattg cttggcaaac agcacaccac cgtcacagct
    15181 tttccagatg tgcggataga tggtgttgcc taaaaccatc cccaacaaga ttgcgagggt
    15241 gagggcacta aacccggcac ccgcaaccgc gggaatggaa ccaccccaca gggcgacccc
    15301 ggtgataact gcactcaggg ctaaccccgg aataaaatgc cacagtgtac gatgttgttt
    15361 ctgtaaggtg atattcgtca taaccctctc ctttaaccgg gcataaggtt acgactaact
    15421 ggtttaaaaa taaaattgat tatatattta taattaatct ttataagtgg taagcgacta
    15481 tgcacatcac cctccggcag ttggaagttt ttgcagaagt attgaaaagt ggatcaacca
    15541 cccaggcgtc ggtgatgctg gcgttgtcgc aatcagcagt gagcgcagcc ttgactgacc
    15601 tggaagggca gcttggcgtt caactgtttg atcgcgtggg gaaaagactg gttgttaatg
    15661 aacacgggcg gctgctctat ccgcgagcgt tggcactgct tgaacaggcg gttgaaatcg
    15721 aacaactgtt tcgcgaagac aacggcgcga ttcgtatcta tgccagtagt accatcggta
    15781 actacattct gcctgcagtt atcgcccgtt atcgccatga ttatccgcag ttgccgattg
    15841 aacttagcgt tgggaacagc caggacgtga tgcaagcggt gctggatttc cgcgttgata
    15901 ttggctttat tgaaggaccg tgccacagca ctgaaatcat ttctgaaccg tggctggaag
    15961 acgagctggt ggttttcgcc gcgccgactt cgccgttggc tcgtggtccg gtcaccttag
    16021 aacagctggc cgctgcgccg tggatcctgc gtgaacgcgg ttccggcacg cgggatattg
    16081 tcgattatct gttgctgtca catttaccga agtttgagat ggcgatggaa ttaggtaact
    16141 ccgaggcaat caaacatgcg gtgcgtcatg ggttgggaat tagttgcctg tcgcgacgtg
    16201 tgattgaaga tcaattgcag gcaggcacat taagtgaagt tgcggtccct ctgccgcgcc
    16261 tgatgcgtac gttgtggcgt atacatcatc ggcaaaaaca cctttccaac gcgctacggc
    16321 gctttctgga ctattgcgat cccgcaaatg tgccgcgtta agttgctgta caagaacatg
    16381 ctggtgctgt gtcgatttcg tgacgcagcg ccttcagcat gcattcgcca gaaaagagat
    16441 tggctgcttt acttataatc cctgggcgat catgaaggtg tcttataacc gtgtatttct
    16501 gccggaagga ttgccaatcg tctgctacaa tcgcgccatc atttttaaga tggatagcat
    16561 ttttgtatgg gttccgaaac taaaactaca gaagcgccgg gcttacgccg tgaattaaag
    16621 gcgcgtcacc tgacgatgat tgccattggc ggttccatcg gtacaggtct ttttgttgcc
    16681 tctggcgcaa cgatttctca ggcaggtccg ggcggggcat tgctctcgta tatgctgatt
    16741 ggcctgatgg tttacttcct gatgaccagt ctcggtgaat tggctgcata tatgccggtt
    16801 tccggttcgt ttgccactta cggtcagaac tatgttgaag aaggctttgg cttcgcgctg
    16861 ggctggaact actggtacaa ctgggcggtg actattgccg ttgacctggt tgcagcacag
    16921 ctggtcatga gctggtggtt cccggataca ccgggctgga tctggagtgc gttgttcctc
    16981 ggcgttatct tcctgttgaa ctacatctct gttcgcggct ttggtgaagc ggaatactgg
    17041 ttctcactga tcaaagtcac gacagttatt gtctttatca tcgttggtgt gctgatgatt
    17101 atcggtatct tcaaaggcgc gcagcctgcg ggctggagca actggacaat cggcgaagcg
    17161 ccgtttgctg gtggttttgc ggcgatgatc ggcgtagcta tgattgtcgg cttctctttc
    17221 cagggaaccg agctgatcgg tattgctgca ggcgagtccg aagatccggc gaaaaacatt
    17281 ccacgcgcgg tacgtcaggt attctggcga atcctgttgt tctatgtgtt cgcgatcctg
    17341 attatcagcc tgattattcc gtataccgat ccgagcctgc tgcgtaacga tgttaaagac
    17401 atcagcgtca gtccgttcac cctggtgttc cagcacgcgg gtctgctctc tgcggcggcg
    17461 gtgatgaacg cagttattct gacggcggtg ctgtcagcag gtaactccgg tatgtatgcg
    17521 tctactcgta tgctgtacac cctggcgtgt gacggtaaag cgccgcgcat tttcgctaaa
    17581 ttgtcgcgtg gtggcgtgcc gcgtaatgcg ctgtatgcga cgacggtgat tgccggtttg
    17641 tgcttcctga cctccatgtt tggcaaccag acggtatacc tgtggctgct gaacacctcc
    17701 gggatgacgg gttttatcgc ctggctgggg attgccatta gccactatcg cttccgtcgc
    17761 ggttacgtat tgcagggaca cgacattaac gatctgccgt accgttcagg tttcttccca
    17821 ctggggccga tcttcgcatt cattctgtgt ctgattatca ctttgggcca gaactacgaa
    17881 gcgttcctga aagataccat tgactggggc ggcgtagcgg caacgtatat tggtatcccg
    17941 ctgttcctga ttatttggtt cggctacaag ctgattaaag gaactcactt cgtacgctac
    18001 agcgaaatga agttcccgca gaacgataag aaataagttt cctcccttcc ttgctaagcc
    18061 ctctcaaccg agagggcttt ttcaattcca tttccctgac aaatcatgcg gatataaaat
    18121 ttaacatttg gattgataat tgttatcgtt tgcattatcg ttacgacgca atcaaaaaag
    18181 gctgacaaat cagaggctgt tccggctttc tgggatgatc acctgcatac aaataagtcc
    18241 accgcgatgc tgccgtacgc aaggggacgt aaagaagatg tgagcgatac ccattttatt
    18301 ttcgtagtta cctcatggag atatggaatg tttaggttga accctttcgt acgggtcggg
    18361 ctgtgtttgt ccgctatttc ttgtgcatgg cctgtgttag cggtcgatga tgatggcgaa
    18421 acgatggttg tcactgcatc ttccgtggaa caaaatctta aagatgcacc cgccagtatc
    18481 agcgtcatta cccaggaaga cctgcagcga aaaccggtac agaatctgaa ggatgtcctc
    18541 aaagaagtgc ctggcgtaca actgacgaac gaaggggata accgtaaggg cgttagtatt
    18601 cgtggtctgg acagcagcta caccctgatt cttgtcgacg gtaaacgcgt taactcccgc
    18661 aatgccgtct tccgccacaa tgatttcgat ctgaactgga tcccggtcga ttccatcgaa
    18721 cgtattgaag tggttcgtgg cccgatgtcg tcgctatacg gctccgatgc gctcggcggt
    18781 gtagtgaata tcatcaccaa aaaaatcggt cagaaatggt cgggtaccgt taccgtcgat
    18841 accaccattc aggaacatcg cgatcgcggt gatacctata acggtcagtt ctttaccagt
    18901 ggaccattaa ttgatggtgt gctgggaatg aaagcttacg gcagcctggc aaaacgtgaa
    18961 aaggatgacc cgcaaaactc aacgaccacc gataccggag aaacgccgcg tattgaagga
    19021 ttctccagcc gcgacggcaa tgtcgaattt gcctagacac cgaatcaaaa tcacgatttt
    19081 actgccggat acggtttcga ccgtcaggat cgtgattccg attcgctgga caaaaaccgc
    19141 ctggaacgcc agaactactg cgtcagccat aatgggcgtt gggattacgg caccagcgaa
    19201 ctgaaatcct acggtgagaa agtcgagaac aaaaaccctg gcaacagcag cccgataact
    19261 tccgaaagca atacggtcga cggcaaatac acgttgccgc tgacggcgat taatcagttt
    19321 ctcacggttg gcggtgaatg gcgtcacgac aaacttagcg atgcggtgaa cctgaccggg
    19381 ggaaccagct ccaaaacgtc tgccagccag tacgcgctgt ttgtggaaga tgaatggcgg
    19441 atcttcgagc cgctggcgct gacgaccggc gtgcgtatgg acgatcacga aacctacggt
    19501 gaacactgga gtccgcgtgc ctacatggtt tataacgcca ccgacaccgt aacggtgaaa
    19561 gggggctggg cgacggcatt taaagcacct tctctgttgc aacttagccc tgactggacg
    19621 agcaattcct gccgtggcgc atgtaagatt gtgggtagcc cggatctgaa accagaaacc
    19681 agcgaaagtt gggagctggg gctttactac atgggtgaag aaggctggct ggaagggggt
    19741 gaatccagcg ttatcgtttt ccgtaacgat gtgaaagatc gtatcagcat cagccgtacg
    19801 tctgacgtca acgctgcacc gggctaccaa aactttgttg gttttgagac gggcgctaac
    19861 ggacggcgca taccggtatt tagctactac agcgttaaca aagctcgtat tcagggcgtg
    19921 gaaaccgaac tgaaaattcc gttcaacgat gaatggaaac tgtcgatcaa ctacacctac
    19981 aacgatggtc gtgatgtcag caacggcgaa aacaaaccgc tatccgatct gccgttccat
    20041 actgctaacg gtacgctgga ctggaaaccg ctggcgctgg aagactggtc attctatgtt
    20101 tctggtcact ataccgggca gaaacgcgcc gacagcgcga cggctaaaac accgggcggt
    20161 tataccatct ggaataccgg cgcggcctgg caggtgacta aagacgtcaa actgcgcgca
    20221 ggcgtgctga accttggcga caaggatctc agtcgtgacg actacagcta taacgaagac
    20281 ggacgtcgtt actttatggc agtggattat cgcttctgat gagaagatgc ccggcgaacc
    20341 gggcggactt tcacttcagt aaatactgcg catggaagcg caggtgatcc tctataaaag
    20401 aggcgatgaa gtagtaactg tgatcatatc ccggctgaat acgcagcgtc atcggccacg
    20461 ctttctggcg cgcggcttct gccaataccg caggttgcaa ctggtcggca agaaactgat
    20521 cattatcgcc ctgatcgata agcgtcggga tcgcatcctg cgcgttactg gcatacatca
    20581 gcgcgcaact atcccattcc agcatgcatt tttgtcctca cctaaatagg tgctaaacgc
    20641 tttgattccc cacgggacgc tgcacggatt cacaatgggc gcaaaggccg aaacgctggt
    20701 gtatttaccc ggatttttca gcgccataat cagcgcaccg tgaccaccca ttgagtgacc
    20761 gctaatggcg cagcggtcgc tgacattaaa ttgcgactga accagcgccg gtaattcatc
    20821 gcgcagataa tcatacatcc ggtaatgcgt cgcccacggc ggttgcgtgg cattaagata
    20881 aaagcctgcg ccctggccta aatcgtagcc atcgtcgttg gcaacctttt cgccgcgcgg
    20941 gctggtgtct ggcatcacca gtacaatccc cagttccgcc gctacccgct gggcacccgc
    21001 cttggtggtg aagttctcgt cattgcaggt taatccggaa agccagtaca gcactggtgg
    21061 cggggtgtga tcacgagggg gggggagaaa gatactgaac gtcatcgggc agtttaaggt
    21121 actggagtcg tgtcgccagc gttgctgcca gccttcaaaa cagcggtgct cttcgagcat
    21181 ttccatgcgt ggctccttgt tgtgttgttt gcaatttctt catcataccg tttattcaat
    21241 gtgctgtgag taactttcac ttccgtattt gcataacgat gttttaacat ctgctgatga
    21301 aaggcagcgg caattacaat aattatcgct gtgaatactg gattatgtgc gccgcctcac
    21361 gcacaataat caagctgtaa atcagcttaa taactttgcc cccacgcagg gtggaggcgt
    21421 cacacctgca ggagaaatca taaatgccat cactcagtaa agaagcggcc ctggttcatg
    21481 aagcgttagt tgcgagagga ctggaaacac cgctgcgccc gcccgtgcat gaaatggata
    21541 acgaaacgcg caaaagcctt attgctggtc atatgaccga aatcatgcag ctgctgaatc
    21601 tcgacctggc tgatgacagt ttgatggaaa cgccgcatcg catcgctaaa atgtatgtcg
    21661 atgaaatttt ctccggtctg gattacgcca atttcccgaa aatcaccctc attgaaaaca
    21721 aaatgaaggt cgatgaaatg gtcaccgtgc gcgatatcac tctgaccagt acctgtgaac
    21781 accattttgt taccatcgat ggcaaagcga cggtggctta tatcccgaaa gattcggtga
    21841 tcggtctgtc aaaaattaac cgtatcgtgc agttctttgc ccagcgtccg caggtgcagg
    21901 aacgtctgac gcagcaaatt cttattgcgc tacaaacgct gctgggcacc aataacgtgg
    21961 ctgtctcgat cgacgcggtg cattactgcg tgaaggcgcg tggcatccgc gatgcaacca
    22021 gtgccacgac aacgacctct cttggtggat tgttcaaatc cagtcagaat acgcgccacg
    22081 agtttctgcg cgctgtgcgc catcacaact gattaaaagg caggaaccat ggagcgcaac
    22141 gtcacgctcg attttgttcg cggcgtcgcc attctgggga tcctgctatt aaacatcagc
    22201 gcctttgggc taccaaaggc ggcttatctc aatcccgcct ggtacgatgc tattacgccg
    22261 caggatgcat ggacctgggc atttctcgat ctcatcggcc aggtgaaatt cctcacgctt
    22321 tttgcgctgc tgtttggtgc tggcctgcaa atgttgctgc cccgtggcag acgctggatc
    22381 cagtcgcggt taacgctgtt agtcttgttg ggctttattc acggtttatt gttctgggac
    22441 ggcgatattc tgctggctta cgggctggtg gggcttggcg ttttgctgtt attggggctg
    22501 atttccgaca gccagaccag ccgcgcctgg acgccggatg catcggctat tttgtatgaa
    22561 aaatactgga agcttcacgg cggcgttgaa gcgatcagta atcgtgccga tggtgttggc
    22621 aacagtttac tggcactggg cgcacagtat ggctggcaac tggcagggat gatgctcatt
    22681 ggtgcggcgc tgatgcgcag cggctggctg aaagggcagt tcagcttacg tcactatcgt
    22741 cgtactggtt ttgtgctggt ggcgattggg gtgaccatta accttcctgc catcgccctg
    22801 caatggcagc tggactgggc gtatcgctgg tgtgccttct tacttcaaat gccgcgggaa
    22861 ctgagtgcgc cgtttcaggc gattggctat gcgtcgctgt tttatggctt ctggccgcaa
    22921 ttgagccgct ttaagctggt gcttgcgatc gcctgcgtcg gacggatggc gctgaccaac
    22981 tatctattgc aaacgctgat ttgtaccacg cttttttacc acctcggttt gtttatgcat
    23041 tttgaccgcc tggagctgct ggcgtttgtt attccggtat ggctggcgaa tatcctcttc
    23101 tctgttatct ggctgcgtta cttccgccag gggccggtgg aatggctctg gcgtcagtta
    23161 actttgcgtg ctgccggacc ggcaatatct aaaacatcaa gataacgatc tggattacat
    23221 tcgttaacaa aacggctgta accgtttcca ttgctgtgac tcgattcacg aagtcctgta
    23281 ttcagtgctg acaaaatagc cgccagcaag cagtcattta ctgcaatctc ataacaggta
    23341 gtgaatatga tcaccattcg tgatgtagcg cgtcaggctg gcgtctctgt ggcaacggtt
    23401 tcccgggtgc tcaataacag cacgctggtc agtgccgaca cgcgtgaagc agtaatgaaa
    23461 gccgtgagtg agctggatta tcggccaaac gccaatgccc aggcgctggc aactcaggtt
    23521 agcgacacca ttggcgtggt ggtgatggac gtttctgatg cgtttttcgg cgcgctggta
    23581 aaagcggtgg atctggtcgc ccagcagcat cagaaatacg tgctaatcgg caatagctat
    23641 catgaagcgg aaaaagagcg tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgcg
    23701 ttgattgttc actcaaaagc attgagtgac gatgaactgg cgcaatttat ggataacatt
    23761 cccggtatgg tgttaatcaa ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg
    23821 gataatctca gcggtgcccg aatggcgacg cgcatgttgc tgaataacgg tcatcaacgt
    23881 attggttatc tttcttccag ccacggcatt gaagatgacg ccatgcgtaa agcaggctgg
    23941 atgagtgcgt tgaaagagca ggatattatt ccgccggaaa gctggattgg cactggtacg
    24001 ccggacatgc cgggcggtga ggcggcgatg gttgaactgc tggggcgcaa tctacaactt
    24061 accgctgtat ttgcttataa cgacaatatg gccgctggcg cactgacagc attaaaagat
    24121 aatggcattg cgattccgtt acatctctca atcatcggtt tcgatgatat tcccatcgcc
    24181 cgttacaccg acccgcaatt aacgaccgtg cgttatccca ttgcttcaat ggctaaatta
    24241 gccaccgaac tggccttgca gggggcagca ggcaatattg atcctcgtgc cagccactgt
    24301 tttatgccga cgttagtgcg tcgccattct gtcgcaacgc gccagaatgc ggcggcgatc
    24361 actaactcaa caaatcaggc gatgtaaccg ctttcaatct gtgagtgatt tcacagtatc
    24421 ttaacaatgt gatagctatg attgcaccgt tttaatgttg taacccgtat gtaacagtga
    24481 ataatcactt tcaccgaggt aatagcgtca taacaacaat taaagccgtt ttctggagcg
    24541 ttaccgggca tggaagaacg aattttaaaa agtgagcttc ggcgttcagt aacacttcat
    24601 taactctact gccccgccga gcatttatct caagcactac cctgcataag aaaaaccgga
    24661 gataccatga ataagaaggt gttaaccctg tctgctgtga tggccagcat gttattcggt
    24721 gccgctgcac acgctgctga tactcgcatt ggtgtaacaa tctataagta cgacgataac
    24781 tttatgtctg tagtgcgcaa ggctattgag caagatgcga aagccgcgcc agatgttcag
    24841 ctgctgatga atgattctca gaatgaccag tccaagcaga acgatcagat cgacgtattg
    24901 ctggcgaaag gggtgaaggc actggcaatc aacctggttg acccggcagc tgcgggtacg
    24961 gtgattgaga aagcgcgtgg gcaaaacgtg ccggtggttt tcttcaacaa agaaccgtct
    25021 cgtaaggcgc tggatagcta cgacaaagcc tactacgttg gcactgactc caaagagtcc
    25081 ggcattattc agggcgattt gattgctaaa cactgggcgg cgaatcaggg ttgggatctg
    25141 aacaaagacg gtcagattca gttcgtactg ctgaaaggtg aaccgggcca tccggatgca
    25201 gaagcacgta ccacttacgt gattaaagaa ttgaacgata aaggcatcaa aactgaacag
    25261 ttacagttag ataccgcaat gtgggacacc gctcaggcga aagataagat ggacgcctgg
    25321 ctgtctggcc cgaacgccaa caaaatcgaa gtggttatcg ccaacaacga tgcgatggca
    25381 atgggcgcgg tagaagcact gaaagcacac aacaagtcca gcattccggt gtttggcgtc
    25441 gatgcgctgc cagaagcgct ggcgctggtg aaatccggtg cactggcggg cacagtactg
    25501 aacgatgcta acaaccaggc gaaagcgacc tttgatctgg cgaaaaacct ggccgatggc
    25561 aaaggtgcgg ctgatggcac caactggaaa atcgacaaca aagtggtccg cgtaccttat
    25621 gttggcgtag ataaagacaa cttggctgaa ttcagcaaga aataagactg attgggcgca
    25681 gtcgtttact gcgcccttat tgtatggcca acaaggtaaa attatatggt cagctcaacg
    25741 actccgtctt ccggggaata cttgttggaa atgagcggta tcaacaagtc ctttcctggt
    25801 gttaaggcac ttgataacgt taatttaaaa gtccggccac attctatcca tgcattaatg
    25861 ggggaaaacg gtgcaggaaa atcgacatta ttaaaatgcc tgtttggtat ttatcaaaaa
    25921 gactccggca ccattttatt ccagggtaaa gagatcgatt tccattccgc caaagaggca
    25981 ctggaaaatg gtatttcgat ggtacaccag gagttaaacc tggtattaca acgttcggtg
    26041 atggataaca tgtggctggg gcgatatccc accaaaggca tgtttgtcga tcaggacaaa
    26101 atgtaccgcg aaaccaaagc gatttttgat gaactggata ttgatatcga tccgcgtgcg
    26161 cgcgtcggca cattatccgt ttcgcaaatg cagatgatcg aaatcgccaa agcgttttcc
    26221 tataacgcga aaattgtgat tatggatgaa ccgacttctt cgttaaccga aaaagaggtc
    26281 aatcatctgt tcactattat tcgtaaatta aaagagcgcg gctgcggtat tgtttatatc
    26341 tcgcataaaa tggaagaaat cttccagtta tgtgatgaag ttaccgtatt gcgcgacggt
    26401 cagtggatcg ccaccgaacc gctggcagga ctgacgatgg acaagatcat cgccatgatg
    26461 gttgggcgtt ctcttaatca gcgttttcct gacaaagaaa acaagccggg cgaagtcatc
    26521 ctcgaggtgc gtaacctaac gtcactgcgc cagccgtcga ttcgcgatgt ctcgtttgat
    26581 ctgcataaag gggagatcct cggtattgcc gggctggtgg gggcgaaacg taccgatatt
    26641 gttgagacgt tatttggtat tcgcgagaaa tcggctggca ccattacgtt gcacggcaaa
    26701 cagatcaata accataatgc caacgaagcc ataaaccacg gatttgcact ggtaactgag
    26761 gagcgccgct caacgggaat ttatgcctat ctggatattg gttttaactc gttaatttcc
    26821 aatattcgca actacaaaaa taaagttggt ttactggata actcgcggat gaaaagcgat
    26881 acccagtggg tgattgattc gatgcgggta aaaacgccgg gtcatcggac gcaaattggt
    26941 tcgctctccg gtggtaatca gcaaaaggtg attattggtc gctggctact aacgcaacca
    27001 gaaatattaa tgctcgatga accgacgcgc ggtattgatg tcggggcgaa gtttgaaatt
    27061 tatcagttaa ttgccgaact ggcgaagaaa ggcaagggga ttattattat ctcctctgaa
    27121 atgcctgagt tgttagggat aacagaccgt attctggtca tgagcaatgg tctcgtttcc
    27181 ggaattgtcg atacaaaaac aacaacgcaa aacgaaattc tgcgtcttgc gtctttgcac
    27241 ctttaagatt aggggcttcc catgagtgcg ttaaataaga aaagttttct tacttacctg
    27301 aaagagggcg gtatttacgt cgttctttta gttttgctgg cgattattat tttccaggac
    27361 ccaacatttt taagtctgtt gaacttaagt aatattctca cccagtcatc ggtgcgtatt
    27421 attatcgcgc tcggtgtggc agggttaatt gtcacccagg ggaccgatct ttctgctggt
    27481 cgtcaggtag ggctggcggc agtggtggct gcgacattat tgcagtccat ggataacgcc
    27541 aacaaagtgt tcccggaaat ggcgacgatg ccgattgcgc tggttattct gattgtctgt
    27601 gccattggtg cggtgatcgg tttgatcaac ggtctgatta tcgcttatct caacgtgacg
    27661 ccgttcatta ccacgctcgg cacgatgatc atcgtctatg gcatcaactc gctctattac
    27721 gactttgtcg gggcgtcgcc aatttctggt tttgacagtg gcttctctac ctttgctcag
    27781 ggctttgtcg cgctggggag tttccgtctc tcttacatca ccttctacgc gttgattgcg
    27841 gtggcgttcg tctgggtgtt gtggaacaaa acccgcttcg gtaagaacat ttttgccatt
    27901 ggcggtaacc cggaagcggc aaaaatatct ggtgtcaacg tcggcctgaa cctgctgatg
    27961 atctacgcgt tgtctggcgt gttctatgcc tttggcggga tgttagaagc cggacgtatc
    28021 ggctctgcca ccaacaacct cggctttatg tatgagctgg atgctatcgc ggcgtgcgtg
    28081 gtaggcggtg tatcgttcag cggcggtgtg gggacggtga ttggcgtggt gaccggggta
    28141 attattttta ccgtcatcaa ctatggcctg acgtatatcg gcgtaaaccc atactggcag
    28201 tacatcatca aaggggcgat tattatcttc gccgtagcgc tggattcact gaaatacgcg
    28261 cgtaagaagt gatgtgattg cggatattgc cggatgcgtc tctggtttta caacgttacc
    28321 gggtgttctt tctcgccttt cttaaacttc acttccccga gctcaataca acccaccggg
    28381 cagacgtgac cacaaagcag acaacccaca catttctcgg tattacaatg cggtgtgcgg
    28441 gttttctcgc tccattccat cgcctggtga ccgccgtcgt aacaggaaat ataacagcgt
    28501 ccacagccaa cacatttgtc aagattgata cggggataga caatataact gcggtctaaa
    28561 tcttccgccg ggacaatatt gttattcgcc agacctacca tctcctgcaa cgaatcaaat
    28621 ccctgatcgg cgagataatg cgataacccg ctcgccatat cttctacgat gcgatagccg
    28681 tactgcataa tgccggtggt gacctgtaac gttgctgcgc cgagcaataa aaactccgcc
    28741 gcatcttccc aggtttcaat gccgccgata ccgctgattg ggaaatcgcg cagttctgga
    28801 tgggtgcgca tttgctgaat gaagcgcagg gcgatcggtt ttaccgcttt accggaatat
    28861 ccggaaatac tcgatttacc gttaacgatc ggcataccga ttttctgatt aagatcgata
    28921 ttggtgatgg atttaacggt gttaattgcc gcaatgccat ctgcgccgcc gcgcttcgcc
    28981 gccagcgcca cttcgcacat atcaccgata ttcggcgtca tcttcgccag cattggcagc
    29041 gtggaacccc gtttcactgc ccgacaatat ttttctacca gctccgggct ttgcccgaca
    29101 tcgctaccca tcgcatgaga agtcatttgc ggacaggaga agttacactc gatcatatcc
    29161 gcgccagctg ctcccattgc tgctcatttt cccccatgat cgaagcgatc aatactttgt
    29221 ccgggtaatc ttccttcagc cgacgcaggg cggtcagatt ctcttccaac ggatgttcag
    29281 caatctgctc catatttttg aagccgataa aaccggtatc ttctttcacc agatgatcaa
    29341 aacgcggcga gacttcgttg gcgataaaaa agccgatcgt tttaaacacc acaccgcccc
    29401 aacctgtgtc gtaggctttg gcacacatct catagcagtt gcctaccggc gaagaagaga
    29461 ggcagaacgg gttgggaaag ttcacgccgc aaaaagtaat cgaaagatct ttcgttaaca
    29521 tgagcaagct ccctctaaat agtgatgaat cgcctcggcg gcttctttcc cggttttcac
    29581 ggcatagacc acggttttgt caccctcaac aatatcgcca gcagcaaaga cttgcggatc
    29641 gcggatctga taattttgtg ttttgatggt gttacgctgc tgctctaact ccgcaaaggc
    29701 atccagtctg gcatgctgac cgacggcgag aataatttta tctgccgcca tcgtcagttc
    29761 gcctgataac cgtacatgct taaacgtgac tttattgcct tccacggcta ctggcgtgaa
    29821 tccatcgatg atcgaaacgc ccagctcccg ggcgctggca aactcttttt cgcttgccgg
    29881 aaactcatct aactcttcac gtgctacgca agttaccgcc tgacagccga gaactttcag
    29941 cgtgctggct acgtccatcg cgacatcacc gccgccgata attaatgcgc tttgtggaat
    30001 gctgatatcg ccttgcgcct gtcgtacacg ttgcaggaag tcgacggcaa tctcaacgtc
    30061 gctatgctca aacagcggta gcccggaacc gcttgataac cccacggtga ccagtaccgc
    30121 gcggttttcc gctttcagtt gctccagggt gagtgtttta ccgatttcgt tattgcactt
    30181 aatggtcacg cccatttttt caatacgggc gatctctgca tccagcactg attgcggtaa
    30241 ccggaattgc ggaataccgt tacgcaacca gccaccgggg tgcgcttctt tctcataaat
    30301 cgtgacgtca taaccctggt ttgtcagtgt cacactggcc tgcaatccgg caggacctgc
    30361 gccaataatc gcgactttgc cgagcgtttt agtaccgggc tgataaattt ccatcccggt
    30421 ttgttgttca aaatcagtaa caaaacgctg taagcggccg atatcaatgg gagcatcaac
    30481 accggcacgg gtacaaccgc tttggcataa tttctccgtc gggcacactc tggcacaaac
    30541 ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg cctttaaaat tacgaaagta
    30601 gattgagcga ataaatttcc ccggatcggt ctgtgccggg caagcctgac tacagggagc
    30661 gtcgtgacat aataaacagc gagaggcttc tttaatcgcc agtaacggcg taaaagccgg
    30721 agtgagttca tccagataat tttgttgcgg catgtgatct tccttaatgc tatgagtaat
    30781 tatggcgaac gctaaataaa tattgcgtgc tgatattaag gctatgcgcg tggggaaatt
    30841 taagctgtga aaggattcac aaatagctgc ggttcagaat gttatatccg cagcttattg
    30901 agtgaagtaa taattattct caataattaa cagaaaagag aaaatattaa cgatagaaaa
    30961 taagataaac aatcatgccg atagataaaa ttccgaagcc aatatatgcc accatataag
    31021 cgaagaagag tccgggattt tttgctcgcc agacatacac cggttccggt gcatttttaa
    31081 cccgtttatc aatcgcgcct gcgcgccagc ctttccatgc ttcgcggaaa caaaatatcg
    31141 ggataaggaa aaaaacggca acgacaaaaa actgctgtac atccataact actttccttg
    31201 tttcttttgt aattcaagta actgttcggg tgtaacggcg ggatacccct gcgcatcttc
    31261 cggcacctgg tgcatagccg gaatagggac cagcggccct aaaaaacgcg gttcacgttt
    31321 aaaaatataa aggtcagcca gcgcaccgaa acgggcggca aattcgcgaa tacgtactga
    31381 cagcatatct ttcggtgacg gtacggcata acactgagcc tgaatcccca tatgcagcgc
    31441 aataaataat gctcgctcac agtggaaacg ttgggtgata ataatgaaat cattagtatc
    31501 gaaaactttg cgtgtacgca cgatggaatc cagcgtacga aagcctgcgt aatcgagaac
    31561 aatatctgat gggtcgacac cagcagcgat taaatctttg cgcatggtca tcggctcgtt
    31621 ataactttgc aatgcgttat cgccgctcag taatagataa tttaccttac cgctgttata
    31681 ggcattaatc gctccttgaa tgcggtagcg ataatactga ttaataacgc cggtacgata
    31741 atattttgct gtgccgagca ccacgccgac ctggcggtag gggagatcct gcaattcgtc
    31801 gtagatataa ggcgcggttt tccagctcat ccagcgatcg aggccgagca cagtcaacag
    31861 cagcaagccg atcaggacta acaggctgag gaacacgcgc tttaacatgc aggagactcg
    31921 agctaaggaa aatttccatc aggctacttt acccgctggt tttgcgcaag aaacggcaaa
    31981 ctgtttgccg ggaatttcga caccaggaga gggctggcaa gccggacaag gcgttcacgc
    32041 cgcatccggc accaggctta agcgagaagc acgcggtcga tactgtgaca gccgagagct
    32101 ttcagcgttg cggaggtggc atcccactga atcaacggcg catcggcttt ttctgccaga
    32161 accgcgcgct ggatatccgg gtaatcataa cccttgagat tcaacagaat taacgccccc
    32221 tgcaacggcg gcagagtcgg gttgaatgcg gcgttttcag cgtagctgcc gctgaaaata
    32281 cgaccgtctt tacattccag cgcgacaccg cttggtgact tactgtaagg catgtgcgaa
    32341 cggtttgccg ctgcaatcgc cgcctgagaa agcgcatcac cagtcagcgc atagccgtga
    32401 tcctgctcgt ccatcagcag cgttttaatc tccagatctt tcggcccaaa ggcatccggc
    32461 agatagtcac gcagcgcgtg tgcctcgcgg cccggcaaat gaatacgcag atccagaccg
    32521 ctgttcagtt cattcataaa ctgacggcag tgaccacaag gcgtatagtt aacggtgatg
    32581 gctgcaagcg ctttttcacc actcaaccaa gcgtggctga tcgcgctttg ttcggcatga
    32641 acggtttgct gcattgttgc accaataaat tccatattgg caccaaaata ccaggttccg
    32701 ctcacaccgc gcgcaattgc gccaacatta aaattcgaca atggcgtacg cgcacaggcc
    32761 gccgccagcg gaagtagtgc gaatgccagc gcgtcttcgt ccagtcccgt tgcgctcttc
    32821 agcgatgaga cttgctcccc ggtcaacaaa gcggggaagt acttgtctgc cagaataggt
    32881 tccagtgcag attgcaagtt atccgcaagt tgggcaaaag cggtttgaaa acgtggatgc
    32941 atggcgttgc ctcataatca tgtaatggga taacagttta cgaacccgtt atggctttat
    33001 atgtgatctg aatctcatta attatgcaac acgtgaaact tacatcaaaa atgcgcgacg
    33061 tatcgcaaat tttagcccat tactgccaga ataatcggga aaaggaacgg tgcgatcagc
    33121 gaagtaatta tcccgcataa caccagcgct agcgaactaa atgcaccttc ctgataatcc
    33181 agctcggcgc agcgcgccgt accgagggcg tgcgaggcag tccccattgc cagaccgcgc
    33241 gcagctttgg tacgaatacg catcgcatta agcaatgtat ggccaaatac cgcgccgagg
    33301 atgccgacga aaatcacgca aactgcgctg attgccggaa taccgccaat actgccgcca
    33361 accgccattg caataggcgt ggtgactgat ttcggcagga ttgacgcggc gatttccggt
    33421 gaagcgccca tcaacaatgc cacggaagtc cccgtcacca tcgcaaccac gctgccgata
    33481 aaacagatgg tgatgatcga tttccagcgc gcgcggatct ggtgtagctg ctcatataaa
    33541 ggataggcca gcgcgacgac cgccggttgc agcagatcgt ttagcacttc gctaccttta
    33601 aagtaactgt cgtaagagat gccggtcagc attaaaaaag gaatgatcac caccattgct
    33661 accagcaacg ggttaagcag gggaaactta taccgtgccg ccagtttgcg ggcggcaaaa
    33721 aagacaatca acgtcagcgg taatgaccac cagatattcg ccatcattct tctgatcctt
    33781 tctgacctac gactttacgt tcaccgtgta ccagttgtga actccagctc accaccagaa
    33841 aaaccaccag cgtactgaca gcacaggaaa ccactaccgg gccaaactgc gcgcggagca
    33901 aatcaaaata ttgcatgacg cctacgccaa tcggcacaaa caatagcgcc atatagcgaa
    33961 tcagtacgta gcaccccgga ttgacccatt tcgccggaag aatttgcaag gccagcagga
    34021 caaacaggat cagcatcccg atgatgctgc ccggaatggt taccggtagc agggaagcaa
    34081 tgaaaatgcc tgcatacagg caggcataaa tcaggacgaa agcgcgtaaa tattgccaga
    34141 taatgttcag tgtcttactc ataatcaaca ccccagatga gatggattca tcatacaatt
    34201 aattcgtaaa acgtgctacc gatcacatta agaaatcagc gcttattttt tcgctacccg
    34261 gtattacgca atgagcagcg aactcaggca tacatcaagc atagagaggg attgcagttt
    34321 gcagcaatcc ctctttgcag acctttaatc cgctgaccat aggcgctaac atagcgcctc
    34381 atttttttgc gggtgatgat atgcgtgtgt tactggcacc gatggaggga gtgcttgact
    34441 ctctggtgcg tgaattgctg accgaagtta acgactacga tctgtgcatc accgagtttg
    34501 tccgcgtggt ggatcaactg ctgccggtaa aagtctttca tcgcatttgc cctgagctac
    34561 aaaacgccag ccggacacca tctggtacgc tggtgcgcgt gcagttgtta ggtcagttcc
    34621 cacaatggct ggcagagaac gccgcccgtg cggtggagtt aggtttctgg ggcgtggatc
    34681 tcaattgcgg ctgcccgtcg aaaacggtta acggtagcgg cggcggggcg acgttactca
    34741 aagatcctga actcatctac cagggggcaa aagcgatgcg tgaagctgta ccggcgcatt
    34801 tgcctgtcag cgtgaaagtg cgtctgggct gggacagcgg tgagaagaaa tttgaaatcg
    34861 ccgatgcggt tcaacaggct ggcgctacgg agctggtggt gcatgggcgg acgaaagagc
    34921 agggttaccg cgcggagcat attgactggc aggcgattgg cgagattcgc cagcggctga
    34981 atattccggt gattgccaac ggtgaaatct gggactggca gagcgcgcaa caatgcatgg
    35041 cgatcagcgg ctgcgacgca gtgatgattg gtcgcggggc gctcaatatt cccaacctga
    35101 gccgggtggt aaaatataac gaaccgcgaa tgccgtggcc ggaggtggtt gctttgctgc
    35161 aaaaatatac ccgtctggaa aagcagggcg ataccgggtt atatcacgtt gcgcggatta
    35221 aacagtggtt gagttatttg cgtaaagaat acgatgaagc aacggaatta tttcagcatg
    35281 ttcgggtgtt gaataactcc cctgatattg caagggctat tcaggcaatt gatatcgaga
    35341 aactctaaaa accatagtga tgtttttgac aatgtgttag ctctttatca ataggccgca
    35401 cggttgtttg gtggcgtcaa aatcgccgag gcgttccctg aaggccgggg cagcccacat
    35461 ggatgtgggc tgagggcgcg ttttacaggg atgttacctc gcgcccgacc cggtagccgt
    35521 aagggataag ccgagggcac cgcgcagcgg cgattttgtt cgccagagcc cgggggggca
    35581 gggggcggcg gcgattggtc gccccctgcg cgctccttgc gccagtgatt atatgaagca
    35641 atgcacatga caggagcgca acaggatgag agactgaaat attctcgtta ttttcggatc
    35701 tgatgacatt aattaatttt aaaatatcat cttaaatacg ccagtcacca ccagtagccc
    35761 aatcaggaaa ataatcaata cagcccagag tataattttc attggcgttc ctttatttgt
    35821 gagtgctgaa taagtatagg aaaaaacgaa gccctttgcc gttatccata aacgattaag
    35881 ataaatcgga agatatcatt tacttagtct atatttttct gggtatgtcg ttttattgct
    35941 actgatgtat tgccacgcga aactaaaaaa tatatcgcaa cacacggtaa tctccgcgca
    36001 ccgtttctta cctgattatt cccccatgaa tcgtgattcc ttttatccag ccatcgcctg
    36061 ttttccgctg ttactgatgc tggccgggtg tgcgcctatg catgaaaccc gccaggcgtt
    36121 aagccagcaa acgcccgctg cacaagttga caccgcatta cccacggcgc tgaaaaatgg
    36181 ttggccagac agccaatggt ggctggagta tcacgataat cagctcactt cattaattaa
    36241 caatgcacta cagaacgcac cggatatgca ggtggctgaa cagcgcattt agctagctga
    36301 agcccaggcg aaggcagttg ccactcagga tggtccgcag atcgactttt cggcggatat
    36361 ggagcggcaa aaaatgtcgg cagaagggct aatggggccg tttgctctga acgatccggc
    36421 cgcaggtacg accggtccgt ggtacaccaa cggaactttt ggcttaacgg cgggctggca
    36481 tctcgatatc tggggaaaga atcgggcgga ggttactgcc cgcctgggta cggttaaagc
    36541 acgggcggcg gaacgcgagc aaacccgcca attgctggct ggcagcgtag cccgcctgta
    36601 ctgggagtgg caaacccagg cggcgttaaa cacggtcttg cagcaaatag aaaaagagca
    36661 gaacaccatt atcgcgaccg atcgccagct atatcagaac gggattactt cttcagttga
    36721 cggtgtggaa accgatatta atgccagcaa aacccggcag cagctcaacg atgtcgcggg
    36781 gaaaatgaaa attattgagg cacggttaaa tgcactcaca aatcaccaga ctaagtcatt
    36841 gaagcttaaa ccggtcgcgt tgccgaaagt ggcaagccag cttcctgatg aactggggta
    36901 ctccttactg gcccggcggg cagatttgca ggcggcgcac tggtacgttg agtcatcgtt
    36961 aagcactatt gatgcggcaa aagcggcatt ttatcctgac atcaacctga tggccttcct
    37021 gcaacaggat gcgttgcact taagcgatct gttccgtcat tccgcgcagc aaatgggcgt
    37081 tacggcaggc ctgacgctac ctattttcga tagtggtcgt cttaacgcca atctcgatat
    37141 cgcaaaagcc gaaagcaact tgtctatcgc cagctacaac aaagcggtgg ttgaagcagt
    37201 gaatgacgtg gcgcgggcag ccagtcaggt tcagacactg gcggagaaaa accagcatca
    37261 ggcgcaaatt gagcgcgatg ccttgcgtgt ggtaggtctt gcgcaggccc gctttaacgc
    37321 gggcatcatt gctggttccc gcgtcagcga agccagaatc ccggcgctgc gtgagcgggc
    37381 caatggcctg ttattgcaag ggcagtggct ggatgcctcc attcgactca ccggtgcgct
    37441 gggcgggggt tacaaacgct gatgatatgc gccttctata cttaacgttt attcagcgtt
    37501 aagtggagaa ctcgatggca taggtcgcga taattaccgc ttccgattcg gggatcggca
    37561 aagagtgcgc gttattactg gcgcagcagg ggtttgatat tggcattacc tggcactcag
    37621 atgaagaagg ggcaaaagat accgcgcgtg aggtggttac ccacggcgta cgtgcggaga
    37681 tcgtgcaact ggatctcggc aatctgccag aaggtgcgca ggcgctggag acactcatcc
    37741 agcgatttgg gcgtattgat gtgctggtga ataatgcggg tgcaatgacc aaagcgccgt
    37801 ttcttgatat ggcttttgat gagtggcgca agatttttac cgttgatgtt gatggtgcat
    37861 ttttatgctc gcaaattgcg gctcgtcaga tggtgaaata agggcagggc ggtcgcatca
    37921 tcaacattac gtcggtacat gaacatacgc cgctgccgga tgccagcgcc tacacagccg
    37981 ctaaacatgc gctcggcggg ttaaccaaaa cgatggcgct ggagttggtc aggcataaga
    38041 ttttggtgaa cgcagtcgcg cctggggcga tagccacgcc aatgaacagc atggatgata
    38101 gcgacgtgaa gcccgacgcg gagccttcga ttcctctacg gcgttttggc gcaacgcatg
    38161 agattgccag cctggtggtg tggctttgtt cggagggcgc aaattacacg acagggcagt
    38221 cgttgatagt ggatggcggc tttatgttgg cgaatccgca gttcaaccca gaatagcgca
    38281 gtgaggtgcg tggatatttc aatgggttag gccagataag acaccagcgt cgcatcaggc
    38341 atggaacacc cggtgccgga tgtggagtga atgcttatct gtggcaactg ctttacgcct
    38401 gattatccgg ctttttcttc ccgcgccgtt tcagccacca gcgcacgccc accaccagaa
    38461 ccacgaccag aatcaaccag acccagtgct tcaaatgctg gtcgagattg tgcaaccacg
    38521 gcgcaatcac ctgaccacca gcgtaaccaa tagtggtaaa aatcaacgcc caggcaaatg
    38581 cgccgagaat attcagcggc agaaagattt tcggcggcag ctggctggca ccaatcagcg
    38641 tcgggccaat cacccgaaag ccatacataa agcgcgtacc aatgacaaac agatacggat
    38701 ggcgttggat aagtttctgt gcccgctcaa ttttatcctg atgtttcgag aaacggcgta
    38761 acagcttgcc gccaaaccgc cgcccgcaca gatagagcac ctggtcgcca atcatgccac
    38821 caagcgccac agaaagtacc accagtggga actttaatag cccctgatgt gccgcaacgc
    38881 ctcccagcaa agtcacggtt tcaccttccg ccaggctacc gataaccagc gcggcataac
    38941 catattgtga gatgagtgta ttgagatcca taggccagtt tgtctccctt tggttgcctg
    39001 taagcataca ccctgtgaat cattcacgct gaaaaaggac atacatcaga cggtgcaaat
    39061 tacatggatg aacaaaaaat aatcacaagc tggcttatac ttgaactgtc aggttcatga
    39121 actcacaaca agggggcggt atgagccatg tctggggact gttttcccat cccgatcgtg
    39181 aaatgcaggt gattaatcgc gaaaacgaaa cgatttctca ccattacacc caccacgttt
    39241 tgctgatggc ggcgattccg gtgatttgcg ccttcattgg cactacacag attggctgga
    39301 attttggcga tggcactatc ctgaagttat cctggtttac cggactggcg ctggctgtct
    39361 tgttttacgg cgttatgctg gctggggttg cggtgatggg gcgggtcatc tggtggatgg
    39421 cgcgcaatta cccgcagcgt ccgtctcttg cgcactgcat ggtcttcgcg ggctacgttg
    39481 caactccgct gtttttaagc ggtctggtgg cgctttatcc actggtctgg ttgtgtgcgc
    39541 tggtcggcac ggtggcgctg ttttacaccg gctacctgct ttaccttggt attccgagct
    39601 tcctgaatat caacaaagag gaaggcctta gcttctccag ttcgacgctg gcgattggcg
    39661 tgctggtact ggaagtgctg ctcaccctaa ccgttatact atggggctac ggttatcgtt
    39721 tgttctagaa agttctttga cgttgcattg ctggcgtaaa tgtcagcaat gcagcattta
    39781 ctcacgattc tcctttgacg atcgcacttt ttgctcgtta tgatggcgtc gctagcctca
    39841 gtaaatcctt actccggcgg tgcgcaaccc gtgcgcgtga accactatct gaatgctcat
    39901 catgccgaaa tttcgagttt ctttatttag cttggccctg atgctggctg tgccttttgc
    39961 accgcaggcc gttgcgaaaa cggcagccgc taccaccgct tcacaaccgg aaattgcctc
    40021 cggtagcgcg atgattgttg atctgaatac caacaaagtg atctattcga accacccgga
    40081 tctggtgcgt ccgattgcgt ctatcagcaa attaatgacc gcgatggttg tgctggatgc
    40141 acgactgccg ctggatgaaa aactaaaagt ggatatcagc cagacgccgg agatgaaagg
    40201 ggtctattcg cgcgtacgac tgaatagcga aatcagccgt aaagatatgc tgttgctggc
    40261 gctgatgtct tcagaaaacc gcgcggcggc aagccttgcg caccattatc ccggtggtta
    40321 caaagccttt attaaggcaa tgaatgcgaa agcgaaatcg ctcggaatga acaacacgcg
    40381 ctttgttgaa cctaccggat tgtcggtgca taacgtttca actgcccgtg acttaaccaa
    40441 actgctcatt gccagcaaac aatatccgtt gatcgggcag ttaagtacta cccgggaaga
    40501 tatggcaacc ttctctaacc cgacgtatac gctgccgttc cgcaatacta atcatctggt
    40561 gtatcgcgat aactggaata ttcagttaac caaaaccggc tttaccaatg cggcgggcca
    40621 ttgtctggtg atgcgtacgg ttatcaataa taaaccggtg gcgctggtag tgatggacgc
    40681 gtttggcaaa tatacccatt ttgccgatgc cagccgcctg cgtacctgga ttgaaaccgg
    40741 taaagtgatg cctgtgccgg cagcagcgtt gagctataaa aaacaaaaag ccgcccaaat
    40801 ggcggcggcg gggcagacgg cacagaacga ttaagtgaat tcggatggcg atactctgcc
    40861 atccgtaatt tttactccac ttcctgccag tttttccgtt tactggtttt accgatcccc
    40921 ggattcatgc tattggtcgg atcattctcg cgatagaact tctgcaacgt ctccggtgct
    40981 ttatacaaat gaccgacgtt atgctcggca gggtactgcg cgccgcgctg ctgtagcagt
    41041 tccagcatct gttcttttaa cgcatgcaca tccacgcctt ttttcactat gtaatcctga
    41101 tggaagacat agcacataaa atggccgtaa tagagcttgt gcaccagctg gctgtcgatc
    41161 tccggcggta aatgctcata ccactcggtg tcgttacgcc gcagagcgat atccagtgcc
    41221 agaatgtctt cgacttcatc ggaatgcacc gcctgataac gaattgctgc gcccgcagcg
    41281 gcgaaacggt gtaaaaacgc tttgctacct tcctccggcg tacagacaaa gaaatcgccc
    41341 tcggcctgtt tgaaataatc caccagccac gatttggctt caccgacgcc atcgcctgcc
    41401 atttttaaca gcagatgatg ctcgtattta tcgcgccagt ttttcatgcg cggcggtaaa
    41461 tggctgggga acaggtgacc gaatttttgc attgcacggt cagtaaaatg cggacggaag
    41521 aatttcactt tctccagcat cgcatcggtg cgtcccttga gattaaagaa gaacggcatc
    41581 ttgtcggtgc ctaacttatc aatcatcagg aaggtgtctt tgccgtattt ttccgcaata
    41641 tcgtagatat cccggtgcat atattccccg gcaaccggca gattttcgaa gttagccaga
    41701 atatgacggc ggatttcggt cagcacttcc ggctggttgg tgccgatata aaacacctgc
    41761 tgattttttt ccgcttcgaa ggtatcaaga cgtaccgcaa agacggccag cttcccggcg
    41821 caaccagaag attcaaataa ccgatccgga tcggcgttat aacgtgcggg cgtgtcggct
    41881 tcaatatcac gaacgcggtg gacatagtca taatcgtggg cgtgacgacc atcgtgacgc
    41941 acatcgtcat ctttgatgcg atcgtcatcc agcttgctaa ggatctgctc cggcgtttcg
    42001 cccagatcaa tccccagatg gttcaccagc gtcagtttgc catcttcatc tatacgcgcg
    42061 aataacgaca tttcggtata cgccgggcca cgttgcacca gcgagccgcc ggagttgtta
    42121 caaataccgc cgatgaccga tgcgcctata cacgatgatc caatcaccga atgcggttcg
    42181 cgtcccagcg gtttgagggc tttttccagc gaatagagcg tggtgcccgg ataggccagc
    42241 acctgttcgc ccttgccgag aacgtgcagc ttgtcgagac gcagggtgct gatgatcacg
    42301 atatcgcgat catagtcgtt accgtttggc gtcgatcctt cggtcaggcc tgtattggca
    42361 gcctgcatca gaataatttt gtcggcggtg acgcaggctt tcagcacccg ccacaattcc
    42421 agtagtgagc cagggaaaac gacagccagc gcgtcgccct gaccagaacg gaagcccttg
    42481 cgatagcggg ccgtttttgc gggatcggtg agcaggtgtg aatgaccgac cagacgggca
    42541 agttcattca aaaaggcttt attatcagtt gttgtcatgg aagacatttt ccactccttg
    42601 tggtggcgaa aaaatatcac aaactagaat agcgccttgt atgacaattc agcgagtgaa
    42661 tattttgaaa attcagacaa taaactgagc attctttttc tctctccatc atgcttatgg
    42721 caaactacgg tttttgcacc attttcgctg ccacgtcggg caacaaagga agaaaaatcc
    42781 atatgaaatg gctatgttca gtaggaatcg cggtgagtct ggtcctgcag ccagcactgg
    42841 cggatgattt attcggtaac catccattaa cgcctgaagc gcgggatgcg ttcgtcaccg
    42901 atctgctcaa gaaaatgacg gttgatgaga aaattggtca gctgcgctta atcagcgtag
    42961 gcccggataa tccgaaagag gcgatccgcg agatgatcaa agacggtcag gttggggcga
    43021 ttttcaacac cgtaacccgt caggatatcc gcgccatgca ggatcaggtg atggaattaa
    43081 gccgcctgaa aattcctctt ttctttgctt acgacgtgct gcacggtcag cgcacggtgt
    43141 tcccgattag cctcggtctg gcctcgtctt ttaacctcga tgcggtgaaa acggtcgggc
    43201 gtgtctctgc ttatgaagcg gcagatgatg gcctgaatat gacctgggca ccgatggtcg
    43261 atgtctcgcg cgatccgcgc tggggacgtg cttccgaagg ttttggcgaa gatacgtatc
    43321 tcacctcaat aatgggtaaa accatggtgg aagcgatgca gggtaaaagc ccggcagatc
    43381 gttactcggt gatgaccagc gtcaaacact ttgccgcata cggcgcggta gaaggcggta
    43441 aagagtacaa caccgtcgat atgagtccgc agcgcctgtt taatgattat atgccgccgt
    43501 acaaagcggg gctggacgca ggcagcggcg cggtgatggt ggcgctgaac tcgctgaacg
    43561 gcacgccagc cacctccgat tcctggctgc tgaaagatgt tctgcgcgac cagtggggct
    43621 ttaaaggcat caccgtttcc gatcacggtg caatcaaaga gctgattaaa catggcacgg
    43681 cggcagaccc ggaagatgcg gtgcgcgtgg cgctgaaatc cggaatcaac atgagtatga
    43741 gcgacgagta ctactcgaag tatctgcctg ggttgattaa atccggcaaa gtgaccatgg
    43801 cagagctgga cgatgctgcc cgccatgtac tgaacgttaa atatgatatg gggttgttta
    43861 acgacccata cagccatttg gggccgaaag agtctgaccc ggtggatacc aatgccgaaa
    43921 gccgcctgca ccgtaaagaa gcgcgtgaag tggcgcgcga aagcctggtg ttgctgaaaa
    43981 accgtctcga aacgttaccg ctgaaaaaat cggccaccat tgcggtggtt gggccactgg
    44041 cggacagtaa acgtgacgtg atgggcagct ggtccgcagc cggtgttgcc gatcaatccg
    44101 tgaccgtact gaccgggatt aaaaattccg tcggtgaaaa cggtaaagtg ctgtatgcca
    44161 aaggggcgaa cgttaccagt gacaaaggca ttatcgattt cctgaatcag tatgaagaag
    44221 cggtcaaagt cgatccgcgt tcgccgcaag agatgattga tgaagcggtg cagacggcga
    44281 aacaatctga tgtggtggtg gctgtagtcg gtgaagcaca ggggatggcg cacgaagcct
    44341 ccagccggac cgatatcact attccgcaag gccaacgtga cttgattgcg gcgctgaaag
    44401 ccaccggtaa accgctggtg ctggtgctga tgaacgggcg tccgctggcg ctggtgaaag
    44461 aagatcagca ggctgatgcg attctggaaa cctggtttgc ggggactgaa ggcggtaatg
    44521 caattgccga tgtattgttt ggcgattaca acccgtccgg caagctgcca atttccttcc
    44581 cgcgttctgt cgggcagatc ccggtgtact acagccatct gaacaccggt cgtccgtata
    44641 atgccgacaa gccgaacaaa tatacttcgc gttattttga tgaagctaac ggagcgcttt
    44701 atccgttcgg ctatggtctg agctatacca ctttcaccgt ctctgatgtg aaactttctg
    44761 cgccgaccat gaagcgtgac ggcaaagtga cggccagcgt gcaggtgacg aacaccggta
    44821 agcgcgaggg ggccacggta gtgcagatgt acttgcagga tgtgacggct tccatgagtc
    44881 gccctgtgaa acagctgaaa ggctttgaga aaatcaccct gaaaccgggc gaaactcaga
    44941 ctgtcagctt cccgatcgat attgaggcgc tgaagttctg gaatcaacag atgaaatatg
    45001 acgccgagcc tggcaagttc aatgtattta tcggcactga ttccgcacgc gttaagaaag
    45061 gcgagtttga gttgctgtaa tgctttagta agggcgcgac gtttgcgccc tttgcctgat
    45121 gttcatatct taacttgctg aattttcgcc ctttgtaggc cggataaggc gctcgcgccg
    45181 catccggcag gaaacgccgg accgaatatg cctcttaagt atttatctga ttctgcaact
    45241 gaatccttcc gctcaggcta actctgtcat tatcaactat gcttttctct taattcgctg
    45301 aaaacaacag taaaagaatg aggaaagcat catgccactc ttaaagctct gggcaggttc
    45361 actggttatg ttggcagccg tgagcctgcc gctacaggcg gcttcccccg ttaaagtcgg
    45421 ttcaaaaatc gataccgaag gcgcgctgct cggcaacatc atcttgcagg tactcgaaag
    45481 ccacggcgta ccgacggtga ataaagtgca actcggaacg actcctgtgg ggcgcggggc
    45541 gattacttcc gatgaactgg atatctatcc ggaatatacc ggcaatggcg ctttcttctt
    45601 taaagatgaa aacgatgcag cgtggaaaaa tgccgggcag ggctacgaga aagtcaaaaa
    45661 gctcgatgcg gagcacaaca agttaatctg gctgacgccc gcgcctgcaa ataacacctg
    45721 gactatcgcc gtgcgtcagg atgtggcaga gaaaaacaaa ctcacttcgc ttgccgacct
    45781 gagtcgttat ctgcaagagg gcggcacctt caaactggca gcctcggcag agtttatcga
    45841 acgcgccgat gcgttaccca cgtttgaaaa agcctatggc tttaagctcg gtcaggatca
    45901 gttgctgtca ctggctggtg gcgacacggc ggtgacgatt aaagccgctg cccagcaaac
    45961 ctctggcgtt aatgccgcaa tggcttacgg cactgacggt ccggtcgcgg cgctggggct
    46021 gcaaacctta agcgatccgc aaggcgtgca acctatctac gcgcctgcac cagtggtgcg
    46081 tgaatcggtg ttgaaggagt atccgcaaat ggcacagtgg ctacagccag tcttcgccag
    46141 cctcgatgca aaaacattgc agcaactgaa tgccagcatt gcagtggaag gactggatgc
    46201 caaaaaagtg gctgccgact acctgaaaca aaaagggtgg gcgaagtaat ttcccgtgac
    46261 ttatctacgt attaatcctg ttctggcgct gctgttgttg ctgacggcaa tcgcagcggc
    46321 gctgccgttt atcagttacg cgcctaatcg tttagtctcg ggtgaagggc ttcatctctg
    46381 gcaactgtgg ccgcaaacga tctggatgct ggtgggcgtt ggttgcgcct ggctgacagc
    46441 ctgttttgtc cccgctaaaa aaggcagcat ttttgcactc attctggcgc aattcgtctt
    46501 cgtattgttg gtgtggggag ctggaaaggc ggcgacccaa ctggcgcaaa atggcagtgc
    46561 gctggcgcgt accagcctcg gcagaggttt ctggctggct gcggcgctgg cattgctggc
    46621 ctgtagcgat gccatccgcc gaatctccac gcatccgctg tggcgctggt tgttgcatat
    46681 gcagattgcc attattccgc tgtggttgct gtactccggc acgcttaacg atctctcact
    46741 aatgaaagaa tacgccaacc gtcaggatgt gtttgacgac gcgctggcac aacatctgac
    46801 gttgctgttt ggtgcggtgc tgcctgcgtt agtgattggt gtgccgttgg gcatctggtg
    46861 ctacttttcc actgcgcggc agggggcaat tttttctctg ctcaatgtca ttcagaccgt
    46921 gccttcggtg gcgctctttg gcctgttgat tgcgccgctt gccgcgctgg ttacggcctt
    46981 tccgtggctg gggaagctcg gcatagcagg aaccggaatg acacccgcac tgattgcgct
    47041 ggtgctctat gccttgctgc cgctggtgcg aggcgtggta gtcggtttga accagatccc
    47101 gcgcgatgtg ctggagagcg ccagagcgat ggggatgagc ggggcgcagc gtttcctgca
    47161 tgttcagtta ccgctggcgt taccggtatt tctgcgcagc ctgcgggtgg tgatggtgca
    47221 aactgtaggt atggcggtga ttgcggcgtt aatcggcgca ggcggttttg gtgcgctggt
    47281 tttccagggg ctgctaagca gcgccattga tttagtgttg ctgggggtga tcccggtaat
    47341 tgttctggcg gtgcttaccg acgcgctgtt cgatttgctt atcgcactgc tgaaggtgaa
    47401 acgtaatgat tgaatttagc catgtcagca aactgttcgg cgcacaaaaa gccgttaacg
    47461 atctcaatct caattttcag gaagggagtt tttcggtgct gattggcaca tctggctccg
    47521 gcaaatccac caccctgaaa atgattaacc gcctggtgga gcatgacagc ggagtgatcc
    47581 gctttgccgg agaagaaatt cgctcgctgc cagtactgga gttgcgccgc cggatgggct
    47641 atgccattca atctattggc ctgttccccc actggagcgt ggcgcaaaac atcgctaccg
    47701 tgccgcaatt acaaaaatgg tcgcgggcgc ggattgacga tcgtatcgac gaattaatgg
    47761 cgctactggg gctggagtca aatttgcgtg agcgttatcc gcatcagctt tccggtggtc
    47821 agcagcaacg tgtgggagtg gcgcgtgcac tggctgccga tccgcaagtc ttactgatgg
    47881 atgaaccttt tggcgcactg gacccggtaa cgcgcggcgc gttgcaacaa gagatgacgc
    47941 gcattcaccg tttgctgggg cgcactattg tgctggtcac tcatgatatt gatgaggcgc
    48001 tacggctggc agaacatctg gtattgatgg atcacggtga agtggtgcag caggggaatc
    48061 cgctgacgat gctgactcgt ccggcgaatg attttgtccg ccagtttttt ggacgtagtg
    48121 aactgggggt gcacctgctt tcgttacgta gcgtggcgga ttacgtgcgt cgcgaagaac
    48181 gggcagaagg tgaggcactg gcagaagaga tgacgctacg cgatgcgctc tccctgtttg
    48241 tcgcgcgggg atgcgaggtg ctgccggtgg tgaacacgca gggccagcct tgcggcacgc
    48301 tgcattttca ggatctgctg gtggaggcgt aagcgtatga agatgttgcg cgatccgctg
    48361 ttctggctca ttgctctttt tgtggcgctg attttctggc tgccttacag ccagccgctg
    48421 tttgctgcct tgttcccaca actgccacga cccgtttatc agcaagaaag ttttgcagct
    48481 ctggcactgg ctcatttctg gctggtggga atttcgagtt tgtttgcggt gatcattggc
    48541 acaggtgccg gaattgctgt tactcgcccg tggggcgcgg aatttcgccc actggtggaa
    48601 actattgccg ccgttggaca gacttttccg cctgtcgcag tgctggcgat tgccgttccg
    48661 gtgatcggct ttggtctgca accagcgatt atcgccttga tcctttacgg tgtgctgccc
    48721 atcctgcagg tgacacttgc cgggctggga gcgattgatc ccagcgtgac agaagttgcg
    48781 aaaggtatgg gaatgagtcg aggtcagcga ctgcgtaagg tcgagctacc gctggcggct
    48841 ccggtgattc tggcgggcgt gcgaacttcg gtgattatca acattggtac ggcgacgatc
    48901 gcctcaacgg taggggccag tacgctgggt acgcccatca tcatcgggct tagcggattt
    48961 aataccgcgt atgtgatcca gggggcgtta ctggtggcac tggcggcgat catcgcagac
    49021 cgcctgtttg aaaggctggt gcaggcgctt agccagcacg caaaataaag gtataacctg
    49081 cgagcatgac gccaccaatt ccgcctaacg ccataaacag gaacagggcg atgaccccaa
    49141 ttttagctat gcgcataatg cactccttat gttaacgaaa ggattgtaca gtaaagcgca
    49201 tttgttaacg aatcattaaa taccgagtgg gaaaatatca tggccttgtt cttgccaact
    49261 ggtgagttgc tgctgttggg cggaggttcg attttcaccg caccacacca gcaatgtacg
    49321 gccttcgaat agttcagggc gtagttgatt gagcgagtgg gcgagcgagg acatcaatgc
    49381 gccatccttg ttgactggca atccagccct ccagccacag acgggtggta tcctgaatat
    49441 tccagccaac caccagcgca tctttaccct gttttttacg tgccgaagcc agacaaatgg
    49501 cgatgtagtt gatcagtacg ccgtcgagga tcgccagcag cgcctggaga gtcggttgtt
    49561 ggcactgaag ccgtcggcgc agaggaataa acagatgtgt ggtgagtgtc tgggcggggt
    49621 aatcctgacc gcgctctttg atccacgttc gcaggctatg tagattgccg
//
LOCUS       DAZXUL010000015        47956 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZXUL010000015 DAZXUL010000000
VERSION     DAZXUL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47956
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.015"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..79
                     /locus_tag="ACH3L1_000811"
     CDS             <1..79
                     /locus_tag="ACH3L1_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075665.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACH3L1_000812"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACH3L1_000812"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFT4075666.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="ACH3L1_000813"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="ACH3L1_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFT4075667.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACH3L1_000814"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACH3L1_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFT4075668.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACH3L1_000815"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACH3L1_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFT4075669.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACH3L1_000816"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACH3L1_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFT4075670.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="ACH3L1_000817"
     CDS             5377..5835
                     /locus_tag="ACH3L1_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFT4075671.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="ACH3L1_000818"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="ACH3L1_000818"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFT4075672.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="ACH3L1_000819"
     tRNA            6887..6971
                     /locus_tag="ACH3L1_000819"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="ACH3L1_000820"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="ACH3L1_000820"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACH3L1_000821"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACH3L1_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFT4075673.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACH3L1_000822"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACH3L1_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFT4075674.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACH3L1_000823"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACH3L1_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFT4075675.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="ACH3L1_000824"
     CDS             complement(10252..13995)
                     /locus_tag="ACH3L1_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFT4075676.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="ACH3L1_000825"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="ACH3L1_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFT4075677.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="ACH3L1_000826"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="ACH3L1_000826"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFT4075678.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="ACH3L1_000827"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="ACH3L1_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFT4075679.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="ACH3L1_000828"
                     /gene_synonym="iatA"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="ACH3L1_000828"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFT4075680.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="ACH3L1_000829"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="ACH3L1_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFT4075681.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="ACH3L1_000830"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="ACH3L1_000830"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFT4075682.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="ACH3L1_000831"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="ACH3L1_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFT4075683.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="ACH3L1_000832"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="ACH3L1_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFT4075684.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="ACH3L1_000833"
     CDS             23392..23499
                     /locus_tag="ACH3L1_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFT4075685.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="ACH3L1_000834"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="ACH3L1_000834"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFT4075686.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /gene="sirB1"
                     /locus_tag="ACH3L1_000835"
     CDS             complement(24538..25347)
                     /gene="sirB1"
                     /locus_tag="ACH3L1_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFT4075687.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /gene="sirB2"
                     /locus_tag="ACH3L1_000836"
     CDS             complement(25351..25743)
                     /gene="sirB2"
                     /locus_tag="ACH3L1_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFT4075688.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="ACH3L1_000837"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="ACH3L1_000837"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFT4075689.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="ACH3L1_000838"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="ACH3L1_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFT4075690.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="ACH3L1_000839"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="ACH3L1_000839"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFT4075691.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="ACH3L1_000840"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="ACH3L1_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFT4075692.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="ACH3L1_000841"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="ACH3L1_000841"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFT4075693.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="ACH3L1_000842"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="ACH3L1_000842"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFT4075694.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="ACH3L1_000843"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="ACH3L1_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFT4075695.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="ACH3L1_000844"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="ACH3L1_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFT4075696.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="ACH3L1_000845"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="ACH3L1_000845"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFT4075697.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="ACH3L1_000846"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="ACH3L1_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFT4075698.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="ACH3L1_000847"
     CDS             36790..39657
                     /locus_tag="ACH3L1_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFT4075699.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="ACH3L1_000848"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="ACH3L1_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFT4075700.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="ACH3L1_000849"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="ACH3L1_000849"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFT4075701.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="ACH3L1_000850"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="ACH3L1_000850"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFT4075702.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATLARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="ACH3L1_000851"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="ACH3L1_000851"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFT4075703.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="ACH3L1_000852"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="ACH3L1_000852"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFT4075704.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /gene="ycgY"
                     /locus_tag="ACH3L1_000853"
                     /pseudo
     CDS             complement(47142..47581)
                     /gene="ycgY"
                     /locus_tag="ACH3L1_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
BASE COUNT        12041 a        12343 c        11742 g        11830 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 gaatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc tggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccga
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgcattaat aaaatg
//
LOCUS       DAZXUL010000016        47663 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZXUL010000016 DAZXUL010000000
VERSION     DAZXUL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47663
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.016"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="ACH3L1_000854"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="ACH3L1_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFT4075705.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACH3L1_000855"
     CDS             complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACH3L1_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HFT4075706.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACH3L1_000856"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACH3L1_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFT4075707.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="ACH3L1_000857"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="ACH3L1_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="ACH3L1_000858"
     CDS             1631..2341
                     /locus_tag="ACH3L1_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFT4075708.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACH3L1_000859"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACH3L1_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFT4075709.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4129)
                     /locus_tag="ACH3L1_000860"
                     /pseudo
     CDS             complement(3469..4129)
                     /locus_tag="ACH3L1_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            4282..6480
                     /locus_tag="ACH3L1_000861"
     CDS             4282..6480
                     /locus_tag="ACH3L1_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFT4075710.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDLMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="ACH3L1_000862"
     CDS             complement(6449..6889)
                     /locus_tag="ACH3L1_000862"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFT4075711.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACH3L1_000863"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACH3L1_000863"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFT4075712.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="ACH3L1_000864"
     CDS             7607..8305
                     /locus_tag="ACH3L1_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFT4075713.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="ACH3L1_000865"
     CDS             8534..9742
                     /locus_tag="ACH3L1_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4075714.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="ACH3L1_000866"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="ACH3L1_000866"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFT4075715.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="ACH3L1_000867"
     tRNA            11849..11925
                     /locus_tag="ACH3L1_000867"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="ACH3L1_000868"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="ACH3L1_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFT4075716.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="ACH3L1_000869"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="ACH3L1_000869"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFT4075717.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="ACH3L1_000870"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="ACH3L1_000870"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFT4075718.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="ACH3L1_000871"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="ACH3L1_000871"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFT4075719.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="ACH3L1_000872"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="ACH3L1_000872"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFT4075720.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="ACH3L1_000873"
     CDS             complement(18540..19811)
                     /locus_tag="ACH3L1_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFT4075721.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20287..20394
                     /locus_tag="ACH3L1_000874"
     CDS             20287..20394
                     /locus_tag="ACH3L1_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFT4075722.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20770..20877
                     /gene="ldrD"
                     /locus_tag="ACH3L1_000875"
     CDS             20770..20877
                     /gene="ldrD"
                     /locus_tag="ACH3L1_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HFT4075723.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21253..21360
                     /locus_tag="ACH3L1_000876"
     CDS             21253..21360
                     /locus_tag="ACH3L1_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFT4075724.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21736..21843
                     /locus_tag="ACH3L1_000877"
     CDS             21736..21843
                     /locus_tag="ACH3L1_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFT4075725.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(21930..23609)
                     /gene="bcsG"
                     /locus_tag="ACH3L1_000878"
     CDS             complement(21930..23609)
                     /gene="bcsG"
                     /locus_tag="ACH3L1_000878"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFT4075726.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(23606..23797)
                     /gene="bcsF"
                     /locus_tag="ACH3L1_000879"
     CDS             complement(23606..23797)
                     /gene="bcsF"
                     /locus_tag="ACH3L1_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFT4075727.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(23794..25365)
                     /gene="bcsE"
                     /locus_tag="ACH3L1_000880"
     CDS             complement(23794..25365)
                     /gene="bcsE"
                     /locus_tag="ACH3L1_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFT4075728.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            25638..25826
                     /gene="bcsR"
                     /locus_tag="ACH3L1_000881"
     CDS             25638..25826
                     /gene="bcsR"
                     /locus_tag="ACH3L1_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFT4075729.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            25838..26590
                     /gene="bcsQ"
                     /locus_tag="ACH3L1_000882"
     CDS             25838..26590
                     /gene="bcsQ"
                     /locus_tag="ACH3L1_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFT4075730.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            26587..29204
                     /gene="bcsA"
                     /locus_tag="ACH3L1_000883"
                     /pseudo
     CDS             26587..29204
                     /gene="bcsA"
                     /locus_tag="ACH3L1_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            29215..31554
                     /gene="bcsB"
                     /locus_tag="ACH3L1_000884"
     CDS             29215..31554
                     /gene="bcsB"
                     /locus_tag="ACH3L1_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFT4075731.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            31561..32667
                     /gene="bcsZ"
                     /locus_tag="ACH3L1_000885"
     CDS             31561..32667
                     /gene="bcsZ"
                     /locus_tag="ACH3L1_000885"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFT4075732.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            32862..33251
                     /locus_tag="ACH3L1_000886"
     CDS             32862..33251
                     /locus_tag="ACH3L1_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HFT4075733.1"
                     /translation="MISNAACKTTVWHSRQRYWKTRANGRRRQHFSGNDWRWAPAAYG
                     LLTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQVKDYAPSHFL
                     LYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            33333..35321
                     /gene="hmsP"
                     /locus_tag="ACH3L1_000887"
     CDS             33333..35321
                     /gene="hmsP"
                     /locus_tag="ACH3L1_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFT4075734.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            35509..36795
                     /gene="dctA"
                     /locus_tag="ACH3L1_000888"
     CDS             35509..36795
                     /gene="dctA"
                     /locus_tag="ACH3L1_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFT4075735.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            37016..38512
                     /locus_tag="ACH3L1_000889"
     CDS             37016..38512
                     /locus_tag="ACH3L1_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFT4075736.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(38608..39537)
                     /gene="kdgK"
                     /locus_tag="ACH3L1_000890"
     CDS             complement(38608..39537)
                     /gene="kdgK"
                     /locus_tag="ACH3L1_000890"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFT4075737.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            39769..40536
                     /gene="pdeH"
                     /locus_tag="ACH3L1_000891"
     CDS             39769..40536
                     /gene="pdeH"
                     /locus_tag="ACH3L1_000891"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFT4075738.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            40606..42666
                     /locus_tag="ACH3L1_000892"
     CDS             40606..42666
                     /locus_tag="ACH3L1_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFT4075739.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(42848..44170)
                     /locus_tag="ACH3L1_000893"
     CDS             complement(42848..44170)
                     /locus_tag="ACH3L1_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4075740.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(44581..45594)
                     /gene="yhjD"
                     /locus_tag="ACH3L1_000894"
     CDS             complement(44581..45594)
                     /gene="yhjD"
                     /locus_tag="ACH3L1_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFT4075741.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(45643..46542)
                     /locus_tag="ACH3L1_000895"
     CDS             complement(45643..46542)
                     /locus_tag="ACH3L1_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFT4075742.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            47062..>47663
                     /locus_tag="ACH3L1_000896"
     CDS             47062..>47663
                     /locus_tag="ACH3L1_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFT4075743.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT        11413 a        11875 c        12828 g        11547 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atctaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtgac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagtg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 gccacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta gaatcagcgt
    20101 caagccggaa ggattccggt gaggcgcaat gttgcggggg cttaatccct ggtggcattg
    20161 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    20221 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    20281 ggctatatga cgctcgcaga gctgggcatg gccttctggc atgatttagc ggctccggtc
    20341 attgctggca ttcttgccag tatgatcgtg aactggctga acaagcggaa gtagcgtgtc
    20401 atgtgggcgt caggctgccg taatggcaat ttgcgcccgg accaggccgc aggggtgaaa
    20461 ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa gcggataata ttctgcggaa
    20521 tgcgcagcac agagtgaaga tgagacggat tccggaacag aaaaacagtc gtagaatcag
    20581 cgtcaagccg gaaggattcc ggtgaggcgc aatgttgcgg gggcttaatc cctggtggca
    20641 ttggttgctg gagagagaaa acccccgcac gttgcaggta tgcacctgac aacaccacgg
    20701 gggccattct tgcatccaaa caatacaaga atagccgcga aacgttgtca ttacaataca
    20761 ggcggctata tgacgttcgc agaactgggc atggccttct ggcatgattt agcggctccg
    20821 gtcattgctg gtattcttgc cagtatgatc gtgaactggc tgaacaagcg gaagtaacgt
    20881 gccatgcggg cgtcaggctg ccgtaatggc aatttgcgcc cggaccaggc cgcaggggtg
    20941 aaactctgcg gcctttttcg taagaaaaat gcaacggttg caagcggata atattctgcg
    21001 gaatgcgcag cacagagtga agacgtgacg gattccggaa cagaaaaaca gtcgtagaat
    21061 cagcgtcaag ccggaagggt tccggtgagg cgcaatgttg cgggggcttt atccctggtg
    21121 gcattggttg ctggagagag aaaacccccg cacgttgcag gtatgcacct gacaacatca
    21181 cgggggccat tcttgaacct gaacaactca agaatagccg cgaaacgttg tcattacaac
    21241 acaggcggct atatgacgct cgcagaactg ggcatggcct tctggcatga tttagcggct
    21301 ccggtcattg ctggcattct tgccagtatg atcgtgaact ggctgaacaa gcggaagtaa
    21361 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg
    21421 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct
    21481 gcggaatgcg cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag
    21541 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
    21601 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
    21661 tcacgggggc taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttggcattac
    21721 aacacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
    21781 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag
    21841 taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
    21901 gaggttaaac tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg
    21961 ttcaggcgaa cgtacggttt atcttggtat tgaataacta ctgcatttga gttctcggag
    22021 accggtgctg tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg
    22081 aaaatcttgc catcaagaac gcgaaccacc agatcggaga tagccaggaa gctgctcggt
    22141 tggtcaatga caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca
    22201 acggggacgt ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg
    22261 tcgcccttca gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat
    22321 ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg
    22381 taatccgctg ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg
    22441 tagaacgtgg cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc
    22501 acggcggtat cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt
    22561 gtttgatcca tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg
    22621 ccgaactggc cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgaaaggtta
    22681 tcaaacagat agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg
    22741 cagctggcgc gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag
    22801 ttcttgaaat caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct
    22861 atatccgacc aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca
    22921 gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc
    22981 agccaggcgt taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact
    23041 accggtgcgc cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca
    23101 gtggtcgtcg gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg
    23161 ttcagccata gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat
    23221 aaccaggcca ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg
    23281 acaaggtcga ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg
    23341 ctttccgggc caggcaacca gatgtcatgc cagaacaaag caaagccgat cggcagggca
    23401 atccagtggc gcaagcgatg caggctgtaa cgcggaatgg gcatcagcag aaacgcggca
    23461 aacaccaaat tgaggagcgg gtggaagtta agatatcccg cccacaacag gccaaactta
    23521 accagaaaat agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc
    23581 atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc
    23641 gtaacgtccc ggccggttta acataacgag gtttagcaaa gaacaaacgt aaggtgtcgc
    23701 gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa
    23761 cgataataat ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc
    23821 taacagtcgc atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat
    23881 gaccggttta gaactttgcg ttaaaggcag cggcattccc cattgttctg gggcaagcag
    23941 gcgcatctgc accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga
    24001 gaaaatgtcg ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat
    24061 ccgacagaat gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt
    24121 acggttaggg cgacatagcg tcagggcttg ctcaacgcgg atacccggta ccggacgtag
    24181 ggcaaccaga acgcctttac cgtgggcagg caagagaggg ttattcatca tgttgttgac
    24241 ggcattacag aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga
    24301 cagcaaggta gtgatatctt ccggtacata gcgactaaac ttctgccctt gcacgctttc
    24361 gatcatcgtc agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca
    24421 ggccaataac aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat
    24481 tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat
    24541 ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt
    24601 gaacaggact tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc
    24661 cagtacagca acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc
    24721 ttcgctttga actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc
    24781 gctgacccct ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa
    24841 acgcaaactg gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt
    24901 atcgttatta tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt
    24961 attcatttta tccaaccatg agcgaagccg ctcggcagga atattttgcc atgcgttatt
    25021 tgcgcaaaca agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc
    25081 caaatagtat agaccttttt catgatttag cattgaaaat aattttattt tttccggccc
    25141 ttgagaatca tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac
    25201 gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg
    25261 gcgatcgacg ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa
    25321 tgatgagata ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct
    25381 gattatcagt ttactagcga aagctaagaa ataaacctac cattgaaatt aaaacttatc
    25441 agatttagca tgtgaatcaa tttagaaggt cacaagacgt gtgattttag tcttttcgtt
    25501 tttaactttt tctgttgcgt aacggaaagt caaaaagtga gcaaattccc gtctcgccgt
    25561 aaacaataaa ccggcgaaat gctcacggtt agaattgtct catgaatggt acggttattt
    25621 catagggatc aagcaaaatg aataacaatg aaccagatac tctgcctgat cccgcgatag
    25681 gctatatctt ccagaatgat attgtggcgt taaagcaggc attttcactg cctgatattg
    25741 attatgccga tatttcccaa cgcgaacagt tggccgcggc attaaaacgc tggccgttgc
    25801 tggcagagtt tgcgcaacaa aagtagggga ttggtgaatg gccgtactgg gattgcaggg
    25861 ggtgcgggga ggcgtgggga caacaaccat caccgccgca ttagcctggt cattacaaat
    25921 gttgggagaa aatgtcctgg tggtcgatgc ctgcccggac aacttgttgc gcctgtcatt
    25981 taatgttgat tttacccacc gtcagggctg ggccagagcg atgctggatg gccaggactg
    26041 gcgtgacgct gggttgcgct acacctcgca gctcgatttg ctgccttttg gtcagttatc
    26101 cattgaagaa caagaaaatc cacagcactg gcaaacccgg ctgagcgata tttgctccgg
    26161 cttacagcaa ctaaaagcca gcgggcgtta ccagtggatt ttaatcgact taccgcgtga
    26221 tgcctcgcag ataacccacc agctgctgag tttgtgcgag cactcgctgg caatcgtcaa
    26281 tgtggatgcc aactgccata tccgactgca tcagcaagcg ctgccggatg gcgcacatat
    26341 tttgattaat gacttccgta ttggcagtca ggttcaggac gatatttacc agctttggtt
    26401 gcaaagccag cgccgattac tgccgatgct cattcatcgt gatgaagcga tggctgaatg
    26461 cctggcggct aagcaaccag taggtgaata tcgcagtgat gcgctggcgg ctgaagagat
    26521 actgacgctg gcgaactggt gcctgttgaa ctactccggg ctgaaaacgc cagtcgggag
    26581 tgcatcatga gtatcctgac ccggtggttg cttatcccgc cggtcaacgc gcggcttatc
    26641 gggcgttatc gcgattatcg tcgtcacggt gcgtcggctt tcagcgcgac gctcggctgt
    26701 ttctggatga tcctggcctg gatttttatt ccgctggagc acccgcgctg gcagcgtatt
    26761 cgcgcagaac ataaaaacct gtatacgcat atcaacgcct cgcgtccgcg tccgctggac
    26821 ccggtccgtt atctcattca aacatgctgg ttattgatcg gtgcatcgcg caaagaaacg
    26881 ccgaaaccgc gcagcgggca ttttcaggtc tgcaaaatat tcgtggacgt taccatcaat
    26941 ggatgaacga gctgcctgag cgcgttagcc ataaaacaca gcatctggat gaaaaaaaag
    27001 agctcggtca tttgagtgcc ggggcgcggc ggttgatcct cggtatcatc gtcaccttct
    27061 cgctgattct ggcgttaatc tgcgttactc agccgtttaa cccgctggcg cagtttatct
    27121 tcctgatgct gctgtggggg gtagcgctga tcgtacggcg gatgccgggg cgcttctcgg
    27181 cgctaatgtt gatagtgctg tcgctgaccg tttcttgccg ttatatctgg tggcgttaca
    27241 cctctacgct gaactgggac gatccggtca gcctggtgtg cgggcttatt ctgctcttcg
    27301 ctgaaacgta cgcgtggatt gtgctggtgc tcggctactt ccaggtagta tggccgctga
    27361 atcgtcagcc ggtgccattg ccgaaggata tgtcgctgtg gccgtcagtg gatatctttg
    27421 tcccgactta caacgaagat ctcaatgtgg tgaaaaatac catttacgcc tcgctgggta
    27481 tcgactggcc gaaagacaaa ctgaacatct ggatcctcga tgacggcggc agggaagagt
    27541 ttcgccagtt tgcgcaaaac gtgggggtga aatatatcgc ccgcaccact catgaacatg
    27601 cgaaagcggg caacatcaac aatgcgctga aatatgccaa aggcgagttc gtgtcgattt
    27661 tcgactgcga ccacgtacca acgcgatcgt tcctgcaaat gaccatgggc tggttcctga
    27721 aagagaaaca gctggcgatg atgcagactc cacaccactt cttctcgccg gacccgtttg
    27781 aacgcaacct gggacgtttt cgtaaaacgc cgaacgaagg cacgctgttc tatggtctgg
    27841 tgcaggatgg caacgatatg tgggacgcca ctttcttctg cggttcctgt gcggtgattc
    27901 gtcgtaagcc gctggatgaa attggcggca ttgctgtcga aaccgtgact gaagatgcgc
    27961 atacttctct gcgtttgcac cgtcgtggct atacctccgc gtatatgcgt attccgcagg
    28021 cggcggggct ggcgaccgaa agtctgtcgg cgcatatcgg tcagcgtatt cgctgggcgc
    28081 gcgggatggt ataaatcttc cgtctcgata acccgctcac cggtaaagga ctgaagtttg
    28141 ctcagcggct gtgctacgtc aacgccatgt tccacttctt gtcgggcatt ccacggctga
    28201 tcttcctgac tgcgccgctg gcgttcctgc tgcttcatgc ctacatcatc tatgcgccag
    28261 cgttgatgat cgccctattc gtgctgccgc atatgatcca tgccagcctg accaactcga
    28321 agatccaggg caaatatcgc cactctttct ggagtgaaat ctacgaaacg gtgctggcgt
    28381 ggtatatcgc accaccgacg ctggtggcgc tgattaaccc gcacaaaggc aaatttaacg
    28441 tcaccgccaa aggtggactg gtggaagaag agtacgtcga ctgggtgatc tcgcggccct
    28501 acatcttcct tgttctactc aacctggtgg gcgttgcggt aggcatctgg cgctacttct
    28561 atggcccgcc aaccgagatg ctcaccgtgg tcgtcagtat ggtgtgggtg ttctacaacc
    28621 tgattgttct tggcggcgca gttgcggtat cggtagaaag caaacaggta cgccgatcga
    28681 accgcgtgga gatgacgatg cccgcggcaa ttgcccgcga agatggtcac ctcttctcgt
    28741 gtaccgttca ggatttctcc gacggtggtt tggggatcaa gatcaacggt caggcgcaga
    28801 ttctggaagg gcagaaagtg aatctgttgc ttaaacgcgg tcagcaggaa tacgtcttcc
    28861 cgacccaggt ggcgcgcgtg atgggtaatg aagttgggct gaaattaatg ccgctcacca
    28921 cccagcaaca tatcgatttt gtgcagtgta cgtttgcccg tgcggataca tgggcgctct
    28981 ggcaggacag ctacccggaa gataagccgc tggaaagtct gctggatatt ctgaagctcg
    29041 gcttccgtgg ctaccgccat ctggcggagt ttgcgccttc ttcggtgaag ggcatattcc
    29101 gtgtgctgac ttctctggtt tcctgggttg tatcgtttat tccgcgccgc ccggagcgga
    29161 gcgaaacggc acaaccatcg gatcaggctt tggctcaaca atgatgataa cgcgatgaaa
    29221 agaaaactat tctggatttg tgcagtggct atggggatga gtgcgttccc ctctttcatg
    29281 acgcaggcga cgccagcaac gcaaccactg atcaatgctg agccagctgt agccgcccag
    29341 acggaacaaa atccgcaggt ggggcaagtg atgtcaggcg agcagggagc tgatgccccc
    29401 atcgtggcgc agaacggtcc ttcgcgtgat gtgaagctga cctttgcgca aatcgcgccg
    29461 ccgccgggca gcatggtgct acgtggtatt aacccgaacg gcagcattga gtttggtatg
    29521 cgcagcgatg aagtggtgac gaaggcgatg ctcaacttcg aatacacgcc atcgccatcg
    29581 ttactgcctg tccagtcgca gttaaaggtt tatctcaatg atgagctgat gggcgtgctg
    29641 ccagtgacca aagaacagtt gggtaaaaaa acgctggcgc aaatgcccat taatccactg
    29701 tttattaccg acttcaaccg tgtgcggctg gagtttgtcg gccattatca ggacgtgtgc
    29761 gaaaacccgg ccagcaccac gctttggctg gatgttgggc ggagcagtgg actggatctg
    29821 acctatcaga ccctgaatgt gaagaatgac ctgtcacact tcccggtgcc attctttgac
    29881 ccgcgcgata accgtactaa caccttgccg atggtctttg cgggtgcgcc ggatgttggg
    29941 ctgcaacaag catctgccat tgtcgcctcg tggtttggtt cgcgttctgg ctggcgtggg
    30001 cagaacttcc cggtgctcta taaccaattg ccggatcgca atgccattgt ctttgcaacc
    30061 aacgacaaac ggccggactt cctgcgcgat catccggcgg taaaagcccc ggtgattgag
    30121 atgattaacc atccgcagaa tccttacgtc aaactgctgg tggtgtttgg tcgtgacgac
    30181 aaagacctgt tgcaggcagc gaaaggtatc gctcagggta acattctgtt ccgtggtgaa
    30241 agcgtggtag tgaatgaagt gaaaccgctg ctaccgcgta agccgtacga tgcgccgaac
    30301 tgggtacgta ccgatcgtcc ggtcacattt ggcgaactga aaacctatga agaacagtta
    30361 caatccagcg gccttgagcc agcagcgatt aacgtttcgc taaacctgcc gccggatctc
    30421 tacctgatgc gcagtaccgg cattgatatg gatattaatt accgctacac catgccgccg
    30481 gtgaaagaca gttcgcggat ggatatcagc ctgaataacc agttcctgca atccttcaac
    30541 ctgagcagca aacaggaggc gaaccgtctg ctgctgcgta ttccggtatt acaaggtttg
    30601 ctggatggca aaacagatgt ctctattccg gcgctgaaac tgggcgcgac caaccagctg
    30661 cgcttcgact ttgagtatat gaacccgatg ccaggcggtt cggtagataa ctgtattacc
    30721 ttccagccgg tgcagaatca tgtggtgatt ggtgacgatt ccaccatcga cttctcgaag
    30781 tattaccact tcatcccgat gccggatcta cgcgcctttg ctaacgcggg cttcccattc
    30841 agccggatgg cggatctgtc gcaaaccatc accgtgatgc cgaaagcgcc taacgaagca
    30901 cagatggaaa cgttgctgaa tactgttggt tttatcggcg cacagacggg cttcccggcg
    30961 attaatctga cggtgaccga tgatggcagc accattcagg gcaaagatgc cgacatcatg
    31021 atcatcggtg gtatcccgga caaactgaaa gacgataagc agatcgacct attggtgcag
    31081 gcgaccgaaa gctgggtgaa aacaccgatg cgccagaccc cgttccccgg cattgtgccg
    31141 gacgagagcg atcgcgcggc agaaacccag tcaacgctga cctcttccgg tgcgatggcg
    31201 gcggtgattg gcttccagtc gccgtataac gaccagcgca gcgtgattgc gctgttggca
    31261 gatagcccac gcggttatga aatgcttaac gatgcggtga acgatagcgg caaacgcgcc
    31321 accatgttcg gttcggtcgc ggtgatccgc gagtccggta tcaacagcct acgtgttggc
    31381 gacgtttatt acgtaggtca tctgccgtgg ttcgagcgct tgtggtatgc gctggcaaac
    31441 catccgattc tgctggcggt gctggcgact atcagtgtga tattgctggc atgggtactg
    31501 tggcgtctgc tgcgaattat tagtcgtcgt cgtcttaacc cggataacga gtaattgaag
    31561 atgaatgtgt tgcgtagtgg aatcgtgacg atgctgttgt tggctgcctt tagtgttcag
    31621 gcagcctgta cctggcctgc ctgggagcag tttaaaaagg attacatcag tcaggaaggg
    31681 cgcgtcatcg accccagcga cgcgcgcaaa atcaccacct ccgaagggca aagttacggt
    31741 atgttctttg ccctggcggc taacgaccgt gcagctttcg ataatattct cgactggacg
    31801 cagaacaatc tcgctcaggg ttctttaaaa gaacgtttgc ccgcctggct gtggggcaag
    31861 aaagagaaca gtaagtggga agtgctggac agcaattcgg cctccgatgg tgatgtctgg
    31921 atggcctggt cgttgctgga ggcggggcgt ttgtggaaag agcagcgtta taccgacatc
    31981 ggcagcgcgt tgctaaaacg tatcgcgcgg gaggaagtgg tgacggtgcc tgggctgggt
    32041 tccatgttgt taccgggcaa agtgggtttt gctgaggata acagctggcg ttttaacccc
    32101 agctacatgc cgccgacgct ggcgcagtat ttcacccgct ttggcgcgcc gtggaccacg
    32161 ctgcgcgaaa ccaatcaaca tttattgctg gaaaccgccc cgaaaggctt ttcgccagac
    32221 tgggtgcgct atgagaaaga caaaggctgg cagctaaaag ccgaaaaaac attgatcagc
    32281 agctacgacg ctatccgcgt ttacatgtgg gtaggcatga tgcctgacag cgatccgcaa
    32341 aaagcgcgga tgctcaaccg gtttaaaccg atggcgacat tcactgagaa aaacggttat
    32401 ccgccggaaa aagtggatgt ggctacgggg aaagcgcagg gtaaaggacc ggtcggtttt
    32461 tctgccgcca tgctgccttt tttacaaaac cgcgatgcgc aggcagttca gcgccagcgc
    32521 gtggccgata actttcccgg cagcgatacc tattacaact atgtgctgac cctgtttgga
    32581 caaggctggg atcaacaccg tttccgcttc tcgacaaaag gtgagttatt acctgactgg
    32641 ggccaggaat gcgcaaattc acactaaaca tattcacgct ttccctcggt ctggccgtca
    32701 tgccgatggt cgaggcagca ccaaccatca gcagaccctg cggatggaca gcggcaacac
    32761 taatgccgtg cgcgggctgg cgaatattta ccgccagcag tcgccagaaa aagctgaagc
    32821 gtttatcgcc tcgctctctg ccagccagcg gcgcagcatt gatgatatcg aacgcagcct
    32881 gcaaaacgac cgtctggcac agcaggcaga ggtactggaa aaccagggca aatgggcgca
    32941 ggcggcagca cttcagcggc aacgactggc gctgggcccc ggcagcgtat ggattactta
    33001 ccgactggca ggaagaagct gcgcggcaat ccaacgatgg cggcagcagt cagggcttcg
    33061 gctacacggc gcgggcatta cttgaacgac gtgttacttc caactggttt gttggcacgg
    33121 caattgatat ccagcaggtg aaagattacg cacccagcca tttcctgctc tacgtacgtt
    33181 attccgccgc cggatggcag ggtgacatgg atttaccgcc gcagccgctg ataccttacg
    33241 ccgactggta agttttcaga tagcgcctct cttaatgccg ctgcgatcgg gtatactcgg
    33301 gcggcaatct gggatttccg gggggagaca atttgcgcgt aagtcgctcg ttaacaatca
    33361 agcagatggc aatggtggca gccgttgtcc tggtgttcgt ttttattttt tgcaccgttt
    33421 tgctgttcca tctggtccag cagaatcgct ataacacggc tacgcaactg gaaagcattg
    33481 ctcgctctgt ccgcgaaccc ttatcttcag ctattttgaa aggcgatatt cccgaagcgg
    33541 aagctattct tgccagcatt aaaccggcag gcgtggtcag ccgtgccgat gtagtgctgc
    33601 ctaaccagtt ccaggcgctg cgtaaaagtt ttattccaga gcgtccggtg ccggtaatgg
    33661 ttactcgcct gtttgagcta ccggttcaaa tctcgctggg cgtctactcg ctcgaacgtc
    33721 cggcaaatcc gcagccaata gcctatctgg tgctacaggc ggattccttc cgtatgtata
    33781 agttcgtgat gagcactctc tcaacgttag tgaccattta cttacttttg tcgcttatcc
    33841 tgaccgtcgc catcagctgg tgcattaacc gcctgatttt gcatccgtta cgcaatattg
    33901 ctcgcgaact taacgccatc ccagcccagg agcttgttgg tcaccaactg gcattaccgc
    33961 gtctgcatca ggacgatgaa atcggtatgt tggtgcgcag ttacaacctc aaccagcaat
    34021 tgctgcagcg ccattatgaa gaacagaacg aaaatgcgat gcgcttcccg gtgtcggatt
    34081 tgccgaacaa agccttgctg atggagatgc tggagcaggt tgtcgcgcgt aaacaaacca
    34141 ccgcgctgat gatcatcacc tgtgaaaccc tgcgtgatac tgcgggcgtg ctgaaagagg
    34201 cgcaacgaga aattctgctg ctgacgctgg tggaaaaact caaatcggta ctgtcgccac
    34261 gtatgatcct cgcgcagatt agcggttatg actttgctgt cattgccaac ggtgtacagg
    34321 aaccgtggca cgcaatcact ttgggtcagc aagtactcac tatcatgagc gagcgcctgc
    34381 cgattgaacg tattcaactc cgtccgcact gtagcattgg cgtggcgatg ttctacggcg
    34441 atctcaccgc cgaacagctt tacagtcgcg ctatttctgc ggcatttacc gctcgccata
    34501 aaggcaagaa tcagattcag ttctttgatc cgcagcagat ggaagcagcc cagaagcggt
    34561 tgacggaaga gagcgatatc cttaatgcac tggaaaatca tcagtttgca atttggttac
    34621 agccacaggt cgagatgacc agcggtaaac tggtcagtgc ggaagtgtta ctgcgtatcc
    34681 agcaaccgga tggcagttgg gacctgccgg atggcttaat cgatcgcatt gagtgctgtg
    34741 ggctgatggt taccgtcggt cactgggtgc tggaagagtc ctgtcgattg cttgcagcct
    34801 ggcaagagcg cggcattatg ctgcccttgt cggtaaacct ctctgcgctg caactgatgc
    34861 acccgaatat ggtggcggat atgctggaac tgttaacccg ctatcgcatt cagccgggaa
    34921 cactgattct ggaagtgaca gaaagccgac gtattgacga ccctcatgct gcggtggcaa
    34981 tcctccgtcc gctgcgcaat gccggagttc gggtggcgct ggatgatttc ggcatgggct
    35041 acgcagggct gcgtcagctg cagcatatga aatcgttgcc aatcgacgta ctgaaaatcg
    35101 acaaaatgtt tgttgaaggc ttgccggaag atagcagcat gattgctgca attatcatgc
    35161 tggcgcagag cctgaactta caaatgattg ccgaaggcgt ggagactgaa gcacaacgag
    35221 actggctggc aaaagcgggc gttggtattg cccagggctt cctttttgct cgcccactcc
    35281 ctattgaaat cttcgaagag agttacctgg aagaaaagta gctaccccaa actgattaca
    35341 aaactttaaa aagtgctggt ttgtgcgagc cagctcaaac tttttaacct ttttgtttca
    35401 attatgatcc aggtacattt ctgtgatgtt gtctgggtgt tattttaagg ccgcaggtac
    35461 ccccccccat aaccttacaa gacctgtggt tttactaaag gacaccctat gaaaacctct
    35521 ctgtttaaaa gcctttactt tcaggtcctg acagcgatag ccattggtat tctccttggc
    35581 catttctatc ctgaaatagg cgagcaaatg aaaccgcttg gcgacggctt cgttaagctc
    35641 attaagatga tcatcgctcc tgtcatcttt tgtaccgtcg taacgggcat tgcgggcatg
    35701 gaaagcatga aggcggtcgg tcgtaccggc gcagtcgcac tgctttactt tgaaattgtc
    35761 agtaccatcg cgctgattat tggtcttatc atcgttaacg tcgtgcagcc tggtgccgga
    35821 atgaacgtcg atccggcaac gcttgatgcg aaagcggtag cggtttacgc cgatcaggcg
    35881 aaagaccagg gcattgtcgc cttcattatg gatgtcatcc cggcgagcgt cattggcgca
    35941 tttgccagcg gtaacattct gcaggtgctg ctgtttgccg tactgtttgg ttttgcgctc
    36001 caccgtctgg gcagcaaagg ccaactgatt tttaacgtga tcgaaagttt ctcgcaggtc
    36061 atcttcggca tcatcaatat gatcatgcgt ctggcaccta ttggtgcgct cggggcaatg
    36121 gcgtttacca tcggtaaata cggcgtcggc acactggtgc aactggggca gctgattatc
    36181 tgtttctaca ttacctgtat cctgtttgtg gtgctggtat tgggttcaat cgctaaagcg
    36241 actggtttca gtatcttcaa atttatccgc tacatccgtg aagaactgct gattgtactg
    36301 gggacttcat cttccgagtc ggcgctgccg cgtatgctcg acaagatgga gaaactcggc
    36361 tgccgtaaat cggtggtggg gctggtcatc ccgacaggct actcgtttaa ccttgatggc
    36421 acatcgatat acctgacaat ggcggcggtg tttatcgccc aggccactaa cagtcagatg
    36481 gatatcgtcc accaaatcac gctgttaatc gtgttgctgc tttcttctaa aggggcggca
    36541 ggggtaacgg gtagtggctt tatcgtgctg gcggcgacgc tctctgcggt gggccatttg
    36601 ccggtagcgg gtctggcgct gatcctcggt atcgaccgct ttatgtcaga agctcgtgcg
    36661 ctaactaacc tggtcggtaa cggcgtagcg accattgtcg ttgctaagtg ggtgaaagaa
    36721 ctggaccaca aaaaactgga cgatgtgctg aataatcgtg cgccggatgg caaaacgcac
    36781 gaattatcct cttaatctca ccacttatgc ccgcactccc ttccccgagt gcgggtgttt
    36841 gcgcataatt gacccctgtg cctgaactta ccacctgggt gagttcaggt ttatttcact
    36901 tcttccgtga cattttcatg ttcttgcggt ctaacacgaa gtgtttttaa cgtcatttag
    36961 actgaccgac aacgggcagt ttttttatta ccaggatagt tgatcagggg ttcacatgca
    37021 gggcataaaa attcgacttt tagcgggcgg tttgctgatg atggccactg ctggctatgt
    37081 gcaggcagat gcgctccagc ctgatccagc atggcaacag gggacgcttt ccaacggttt
    37141 acagtggcaa gtgctgacca ccccccagcg tcccagcgat cgtgttgaaa ttcgcctgct
    37201 ggttaatacc ggttcgctcg ccgaaagtac acaacagagc ggttacagtc acgccatccc
    37261 tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagca caggcgcgtt cattgtggca
    37321 gcaggggatc gaccctaaac gcccgatgcc gccggtaatt gtctcttatg acaccacgct
    37381 gtttaatctg agtttgccca ataaccgtaa cgatttgctg aaagaagcgc tctcttatct
    37441 ggcaaatgcc actggcaaat tgaccatcac accagaaacc atcaaccacg cgctgcaaag
    37501 tcaggacatg gtggcaacct ggcctgccga tactaaagag ggctggtggc gctatcgtct
    37561 gaaagggtca accttgttag gtcacgatcc tgccgatccg ctgaaacaac ccgttgaagc
    37621 ggaaaaaatt aaagatttct atcagaaatg gtacaccccg gatgcaatga cgctactggt
    37681 ggtgggaaac gtggatgcgc gctcggttgt cgaccaaatc aacaaaacgt ttggcgaact
    37741 gaaaggcaaa cgtgaaacgc cagctccggt gccgacgctt tctccgctgc gtgcggaagc
    37801 ggtgagtatt atgactgacg cggtgcgtca ggaccggtta tctatcatgt gggatacgcc
    37861 gtggcagccg attcgtgaat cagccgcact gctgcgctac tggcgtgcgg acctggcccg
    37921 cgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc agtgacagca aagacatcgg
    37981 tcttggattt gactgccgtg tgctgtatct gcgtgcgcag tgtgccatca acatcgaatc
    38041 accaaacgac aagctgaaca gcaaccttaa tctggtggcg cgtgaactgg cgaaggttcg
    38101 cgataaaggt ctgccggaag aagagttcaa tgcgttagtg gcgcaaaaga aactggagct
    38161 gcagaaactg tttgccgcct atgcacgggc tgataccgat attctgatgg gccagcggat
    38221 gcgttcgttg caaaatcagg tcgtcgatat cgcgccggag cagtatcaga aactgcggca
    38281 ggatttcctt aacagcctga ctgtggagat gttaaatcag gatctgcgtc agcagttgtc
    38341 gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag ccggaattta acatgaaagc
    38401 gttgcaggcg gcctgggatc aaatcatggc cccatctacc gccgctgcga ccacctctgt
    38461 cgccacggat gacgtacatc ctgaagtgac ggatattcca cctgcacagt aatgcctgat
    38521 ggtgatgagt tagcacaacc atcaggtatt tatggatgag ctggatagag gggtaacact
    38581 ttatccagcc ttttgcatat gctgcgttta cgctggcatc gcctcacgcg ggataatcgc
    38641 gccgcgatac tgaataacgg tacttgcggt caggtgcccg cgtttcgccg cgttttccgc
    38701 actgccgccc gtcaaacgta ccgccagata accggcgctg aaagagtcgc cagctgcggt
    38761 ggtatcgatc actttttctt tcggcagttt caccgccgga acatcaacta acgcttcacc
    38821 agcaatggac accaggcaag aatccgcccc gcgtttcacc accacttctt tcacgcccgc
    38881 gttatgggtg cgcgcaatga cgtcttccac cggctgttga ccccacagcg cgtcttcgtc
    38941 gtccagcgtc aggaaggcga tatccgtgca ttccagcatt tgctggtaca cctgctgtgt
    39001 ctcttctttg ctggcccaca ggcgcggacg atagttattg tcgaaaatca cttttccgcc
    39061 tttggcgcgg cattcgcgca gcagggaaag cagcttttcg cggctggtcg ggcttaagat
    39121 cgccaggcta atcccgctca ggtagagata atcgaaattc gccagctctt cgcaaatcgc
    39181 cgcagactgc tcgctcgcca gccagaattt ggcggcggct tcgttacgcc agtagtagaa
    39241 cgtacgttcg ccggtgctgt cggtttcaat gtagtaaagg cccggaagac ggttttccat
    39301 ccgttgggtc agggaagtat caacgttctc gccgtgccag gcgtccagca tctgctggct
    39361 aaaactgtcc gttcccagcg ccgttacgta atgaacggtt aatgccgcag gatcgacctg
    39421 acgggcgata tagacggaag tgttcagggt atcgccgccg aaaccgcgct taacgtccgc
    39481 gcctttctcg gaaagctcaa tcatgcattc gccaatcacg gcaatctttt tggacatagt
    39541 cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct gtggtggttt gctggtcaac
    39601 catattaaaa cagtgttcca ttattttttt gagccagaac gtattcctga aagattccgt
    39661 tgtggagtga ggaaatttac cccggccagg cggtaaagtt ctgcccttac gcgccgataa
    39721 tctttgtcga gtccgggcag catcactttt aaacacagga catctttgat gataaggcag
    39781 gttatccagc gaataagcaa ccctgaagca agcatcgaga gcttgcagga acggcgtttt
    39841 tggttgcagt gtgagcgtgc ttacacctgg cagccgatct atcaaacatg cgggcggtta
    39901 atggccgtgg agctattaac ggtggtcacg catcccttga acccttcgca acgcctgccg
    39961 ccggatcgct attttactga aatcaccgtc agccatcgga tggaggttgt gaaagagcag
    40021 attgatttgc tggcgcaaaa agccgacttc tttgtagagc acggcctgct ggcatcggtc
    40081 aatattgatg gccctacgct catcgccctg cgtcagcaac caaaaatcct gcgccagatt
    40141 gagcgtcttc cctggctgcg tttcgaactg gtggagcata tccgtctgcc gaaagattca
    40201 acctttgcct cgatgtgtga atttggcccg ctgtggctgg atgattttgg taccgggatg
    40261 gcaaatttct ctgcactaag tgaagtgcgt tatgactaca tcaaaatcgc gcgagaactg
    40321 tttgtgatgc tgcgtcagtc gccggaaggc cgcacactct tttctcagct tttacatcta
    40381 atgaatcgct attgtcgcgg ggtgattgtc gagggcgtag aaacaccgga agagtggcgt
    40441 gatgttcaga actcgcccgc attcgccgca caaggctggt ttctttcacg cccggcaccg
    40501 atggaaacgc tgaatacggc ggttctggcg ctataagctg cctcattttc cgccgggctg
    40561 gactatcttt aggactggca caggaagaat atgaggaagc gaacgatgag caaggcaggc
    40621 aaaataaccg ctgcgatttc aggggctttc ttgttgttga ttgtcgtggc gatcattttg
    40681 attgcaactt ttgactggaa tcgactcaaa ccgaccatca accagaaagt ctctgcggag
    40741 ttgaatcgtc cgttcgctat ccgtggcaat ctgggcgtgg tgtgggagcg gcaaaaacaa
    40801 gaaactggct ggcgcagctg ggtgccgtgg cctcatgtac atgcagaaga catcattctt
    40861 ggtaatccac cggatattcc cgaagtcacg atggtgcatt tgccgcgagt agaggcaacg
    40921 ctggcaccgc tggcgctgct gaccaaaacg gtctggctgc cgtggatcaa gctcgaaaag
    40981 cccgacgcgc gcctgattcg cctctctgaa aagaacaata actggacgtt taatcttgcc
    41041 aacgatgaca acaaagacgc gaatgcaaag ccgtcggcat ggtcgtttcg gctggataat
    41101 attcttttcg atcaagggcg gatcgccatt gatgacaaag taagcaaagc ggatctggaa
    41161 atttttgttg atccgttagg caagccgctg ccattcagcg aagttactgg atcgaaaggt
    41221 aaagcggata aagaaaaggt gggcgattac atttttggcc tgaaggcgca gggacgttat
    41281 aacggtgaac cgctcacggg tacgggaaaa ataggcggta tgctggcgct gcgtggcgaa
    41341 gggacgccgt ttccggtaca ggctgatttc cgttcaggta atacccgtgt tgcttttgat
    41401 ggcgtcgtga atgacccaat gaagatgggc ggtgtcgatt tacggcttaa attttctggc
    41461 gattcactgg gtgatctcta tgaactgacg ggcgttctgc tgcccgatac cccgccgttt
    41521 gaaacggatg gtcggctggt agcgaaaatc gacactgaaa aatcgtcggt ctttgattat
    41581 cgcggtttta atgggcgaat tggcgatagc gatatccacg gttctctggt ctacaccacc
    41641 ggaaagccac gaccaaaact ggaaggtgat gtcgagtcgc ggcaattgcg gctggcggac
    41701 ctgggaccgt tgattggcgt tgattccggg aaaggggcag aaaagtcgaa acggtctgaa
    41761 cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag tgctgcctta tgaccgcttc
    41821 gaaaccgata aatgggacgt tatggatgcc gatgttcgct tcaaagggcg gcgcattgag
    41881 catggcagta gcctgccgat tagcgatctt tctactcata tcatcctcaa aaatgctgac
    41941 ctgcgcctgc aaccgctgaa atttggcatg gcgggcggca gcattgcggc gaatattcat
    42001 ctggaaggcg ataaaaagcc gatgcagggg cgggcagata ttcaggctcg tcgactgaaa
    42061 ctgaaagaac tgatgcccga tgtggaactg atgcagaaga cgctggggga aatgaacggt
    42121 gacgcggaac tacgcggtag cggtaactcg gtggcggcgc ttttaggcaa cagtaacggc
    42181 aacctgaaac tgttgatgaa tgacgggctg gtgagccgca acctgatgga gattgttggg
    42241 ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg acgatgaggt gcgggtgaac
    42301 tgcgcggcgg cgaatctgaa tattgccaac ggcgtggcgc gcccgcagat ttttgctttc
    42361 gatactgaga acgcgttgat taatgttacc ggcacggcaa gttttgcttc ggaacagctg
    42421 gatttgacta ttgatccgga gagtaaaggg attcggatta tcacactgcg ttcgccgctg
    42481 tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga aagccggacc gctgattgcc
    42541 cgtggtgctg ttgctgcggc actggcaacg ctggtaacac cggcggcggc gttactggca
    42601 ctgatctcac cttccgaagg ggaggctaat cagtgtcgga cgatattgtc gcagatgaag
    42661 aagtgatgat aattgccgga tgtctgacat ccggcaatta aacagattta gatttttggc
    42721 aaagtgtgct ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaatcctt
    42781 gtgaattcaa tatattgtat ggaaattcag tcctgataag cgtagcgcat caggcttttc
    42841 acacttatta caacgactga tgtcgcgtct catgggtcag cagcagggcg attaacgtca
    42901 agccagccat cgccgccaga tataacccca ccgcacctaa cccgtagtta gcctgcaacc
    42961 aggctgcgat atacggcgca acggaagccc cgagaatcga cgatacgttg taagagaacg
    43021 atgctccggt gtaacgcact tctgtcggaa acagctctgg taacagcgca cccatcggcc
    43081 cgaaggtcag ccccatcaga cttaacccca gcagcaggaa ggcaaaaacc agaatcgggt
    43141 tgccagaacc gagcagcggg ttaaaggcga acagcgcgaa caggatgatc agcgtggtga
    43201 tgatcaccat gcttttacgg cgaccaaagg catcagccag taatccggcg actggcacca
    43261 tcacgccaaa accaataact gccatcatca gcatccacaa cacttcgtta cgcggcaggc
    43321 caagcccaac tggcgcggcg gcggtactga aggtcataga gtagaccgtc atgatgtaaa
    43381 acagcgtata ggttgccagc ataatgaacg tacccagtac ggttacgcga acatgtttgg
    43441 tcagcagcgt acccagcggg atcttcacct gttttttcgc tttagcgact ttctcaaaca
    43501 ccggcgactc atgcagcgac acgcgaacat acaggccgat aatgaccagc accgccgaga
    43561 agataaatgg cacacgccag ccccagctca taaactgctc atcggtcagt agccaggaaa
    43621 gcagcaagaa agtgccgttg gcaaagaaga agccaatcgg tgcgcctagc tgtgggaagg
    43681 agccatacag tgcacgtttg cgcggtgggg cgttttcagt cgccagcagc gccgcgccgc
    43741 cccattcacc gcctaagccc agaccctgac caaatcgagc cagcgccagc agcagtgggg
    43801 cgaaaatacc aatcgtggca tagcccggca gcagaccaat caccacggtc gaaatcccca
    43861 tcgttagcaa cgaggcgacc agcgtcgctt tacgcccaac gcgatcgcca aaatgaccaa
    43921 aaacggcaga gccaatgggg cgcgcgacga aggcgatggc gaaggtggcg agcgactgta
    43981 gcgttgctgc cgttggatcg ccctgcggga agaagatatg cggaaacaca ataacggccg
    44041 cagtggcgta aatgtaaaag tcgaagaact caatggccgt gccaatgaga gaggcgacaa
    44101 ggactttatt acgcgagttg atcggcgtgt attcttgttc gtggtcgagt gttgtggctg
    44161 ttgcttgcat aatcttttct tattttttgg ctaacgaata gccatattac gcacagcaaa
    44221 agtgatattt caatgtgtaa caaatcgcgg aaatggtcaa aaaccaggca aaaaggggat
    44281 aatgtttagg ccagtgattt ttcttctatg aaatgcttca cataatttta taaatagtgg
    44341 ataaaactgg tttcaggtta aataaaagtt accaactgga tttatatgct ggggttatga
    44401 gttggagtga aattgtttgc tcccaaatag atggctgaag aggctctggg gaagagtttc
    44461 aagaggttat gtgcgcatta tagccggatg cgatgctggc gcatcttatc cggcctacgg
    44521 ggagccaggc cgaataaaat cgtatgccgg ataaggcgtt tacgccgcat ccggcctatt
    44581 ttaaggctgc gttttccctg gcattcgcgg gtcgtcttta tattcggcgg tggcaatcca
    44641 cgccgcgcaa aacagcgtca aacgggcgaa gaagtagaaa aatgccatca gccccagcac
    44701 ggaaccaaat gctgcgccag agggggattt catcagcgat ggcagggtgt aggtcatcac
    44761 gattttaatc acttcaaaac caatagcagc cagaaatgtt ccgcgaatca gggctttttt
    44821 gcgcggacgg tggcgcggca accgccagaa gatccagaag aaaagcagat agttggcgaa
    44881 gatggaaatc gccaggccaa tcaatcgcca cgtcggtttc agccactcaa tgctgttcag
    44941 gtgcagggcg ctaataatca tttgctgcgc cgaaccggca accgaggtga tcgaaagcgt
    45001 cacaatcagc gcaatcaaca aaccaatcag tgaaataaaa tcacgcagat attttaccca
    45061 gaacttctcc tgatcttgcg gcgagcgttc ccagacatcg cgcgactggg cacgaatcgc
    45121 ttcacgcagg ttacccatcc agttgatgcc ggaataaagt gccaccgcca ggccgacaag
    45181 ccctacagtc gtacgctgct gaacggcggt gttgatggtg tttttcaacg tggcggctag
    45241 tgtcggatcg ctgatgtttt gcagaatttt gtcgaagata tcctgtagca acatcggatg
    45301 ggaggccagc acaaagcccc ctgcggcaaa cgacaccatc aaaatcggga tcatcgacaa
    45361 aaatgagaaa taggtgatag ccgcaccaaa ctggttaccc agccgatcgt taaagcgttc
    45421 tgtcgcgcga ataaggtgcg caataaccgg ttggcgttgg actttttcgg cagtggtggt
    45481 gacggtttcc agcgcctggc taactttgcc gcctttctcg ctattatcca actgcttaat
    45541 cggctcgtgc tccagatcct gggtgggacg tttgatctcg ttttcctgcg tcatccgctc
    45601 ttttgtcctt ctgtcgtggt cattcttaaa aagtatagtc ggtcagtcca ggtgctcttt
    45661 cattacgcca gccagccact gcataaacag gtttacacgc cgggaaagct cccgacgctg
    45721 cgggtaaacc agcgaaaggg agagcggctc ggcacggtag cctggcaaca cttcaataag
    45781 ccgcccggca cgcagggctt cgcgcacggc aatgcgcggg atctgaataa tccccagacc
    45841 ggcaagaccg gcggcgagat agttttcgct actgtttacc gtcaacatgc cgccagactt
    45901 aaaccactgg acgccattaa cgctggcaac ctcaaaacct aacggatgta cacccaggtg
    45961 cggtgtataa cgcactattg cgtgtgaagt cagatcatcg gggctttgcg gataaccaaa
    46021 gcgcgtcaga tagtgcggac tggcacagtt gaccatggtt agtttgccga ggggacgggc
    46081 gataacgcca tcttccggta acgcgccagt gcgtatcacg caatcaaaac catcatgaag
    46141 aatatctacc ggacggttat ggctactcag ttccagctca attcccggat gctgatagag
    46201 aaattccgac agacgcggca gtaacaggtt tttcgcgatt cctgacggga tgtcgatgcg
    46261 taatttaccg ctgatactgg tggcatcctg ttgaaacaga ccgtccagtt cgctgagatt
    46321 actcaacaca tcttttgccc gttgataata ggtcatgcct tctggcgtga gtttgacccg
    46381 tcgcgtagtg cgctggagaa gctgggtgcc aagttgatgt tccagtgcct gtatctggcg
    46441 cgaaacactt ccctttggca aagcaaagaa atccgccgcg cgggaaaaac tttccagctc
    46501 cgcgactttg atgaacaact gcattgcgtg aattttatcc atttctttga ccgattgttg
    46561 ttcacagcga aacagtaacg cgcatatcgc attgtttatt gttttttcta acatctaatg
    46621 ccgggtttca caatgttgtc atgacgaatg tggcgatttc tcagcaggaa aagggcggaa
    46681 tagctttatg agaaaggtga atccccttaa acagagcgta tttttcagtg attcccttta
    46741 aagtgaatag aggacgtatt ggctatttgt gcttattttt gtctcattgc catttgttat
    46801 tttggcttgt agtcgcggaa tttcattttt aattgcatgc gtactaacca ccaatataag
    46861 gaaatcctca aaattgtttt aaatagtgcc ctcgacatgt aatttcaggc gtttagctgt
    46921 aactatttat atcggtaact attttctacg cgtcatctct gtagaatggc caccgccaaa
    46981 tggcgaattc ttattttttc tcgcgggtaa tacccttcca ctacttaagt ctttttaacg
    47041 tattcaggtg gagattcagt tatgcaaata gtcatgtttg acaggcagtc aatatttatt
    47101 catggaataa aaatcagttt acagcagcgt attccaggag tgagtattca gggggtcagt
    47161 caggcagacg agttatggca aaagctggaa agttaccctg aagccttagt tatgctcgat
    47221 ggcgatcagg atggtgagtt ttgctactgg ttgctgcaaa aaaccgtggt gcagtttcct
    47281 gaggttaagg tgttaatcac ggcgacggat tgcaataaac ggtggttaca ggaagttatc
    47341 cattttaatg tgctggccat tgtgcctcgc gattcaaccg tcgagacatt tgcgttggcg
    47401 gtaaatagtg cggcgatggg gatgatgttt ctgcctggag actggcggac gacgccggaa
    47461 aaggacatta aagatctcaa atcgttaagc gcccgccagc gggagatttt aaccatgtta
    47521 gcggcgggcg aatcaaataa ggagatcggc agagcgctga atatcagtac cggaacggta
    47581 aaagcgcatc tggaatccct gtatcgccgt ctggaagtga aaaatcgcac ccaggcggca
    47641 atgatgttaa atatctcctc ctg
//
LOCUS       DAZXUL010000017        46496 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZXUL010000017 DAZXUL010000000
VERSION     DAZXUL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46496
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.017"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(114..1973)
                     /gene="gss"
                     /locus_tag="ACH3L1_000897"
     CDS             complement(114..1973)
                     /gene="gss"
                     /locus_tag="ACH3L1_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFT4075744.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2178..3044
                     /gene="yghU"
                     /locus_tag="ACH3L1_000898"
     CDS             2178..3044
                     /gene="yghU"
                     /locus_tag="ACH3L1_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFT4075745.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3073..3154)
                     /locus_tag="ACH3L1_000899"
                     /pseudo
     CDS             complement(3073..3154)
                     /locus_tag="ACH3L1_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3167..3415)
                     /gene="hybG"
                     /locus_tag="ACH3L1_000900"
     CDS             complement(3167..3415)
                     /gene="hybG"
                     /locus_tag="ACH3L1_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFT4075746.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3549..3890)
                     /gene="hypA"
                     /locus_tag="ACH3L1_000901"
     CDS             complement(3549..3890)
                     /gene="hypA"
                     /locus_tag="ACH3L1_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFT4075747.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3883..4371)
                     /gene="hybE"
                     /locus_tag="ACH3L1_000902"
     CDS             complement(3883..4371)
                     /gene="hybE"
                     /locus_tag="ACH3L1_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFT4075748.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(4364..4858)
                     /locus_tag="ACH3L1_000903"
     CDS             complement(4364..4858)
                     /locus_tag="ACH3L1_000903"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFT4075749.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4858..6561)
                     /gene="hybC"
                     /locus_tag="ACH3L1_000904"
     CDS             complement(4858..6561)
                     /gene="hybC"
                     /locus_tag="ACH3L1_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFT4075750.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6558..7736)
                     /gene="hybB"
                     /locus_tag="ACH3L1_000905"
     CDS             complement(6558..7736)
                     /gene="hybB"
                     /locus_tag="ACH3L1_000905"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFT4075751.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7726..8712)
                     /gene="hybA"
                     /locus_tag="ACH3L1_000906"
     CDS             complement(7726..8712)
                     /gene="hybA"
                     /locus_tag="ACH3L1_000906"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFT4075752.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8715..9833)
                     /gene="hybO"
                     /locus_tag="ACH3L1_000907"
     CDS             complement(8715..9833)
                     /gene="hybO"
                     /locus_tag="ACH3L1_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFT4075753.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(10022..10309)
                     /gene="yghW"
                     /locus_tag="ACH3L1_000908"
     CDS             complement(10022..10309)
                     /gene="yghW"
                     /locus_tag="ACH3L1_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFT4075754.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10428..11315)
                     /gene="yghX"
                     /locus_tag="ACH3L1_000909"
     CDS             complement(10428..11315)
                     /gene="yghX"
                     /locus_tag="ACH3L1_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFT4075755.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11472..12512
                     /gene="gpr"
                     /locus_tag="ACH3L1_000910"
     CDS             11472..12512
                     /gene="gpr"
                     /locus_tag="ACH3L1_000910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFT4075756.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12552..13046)
                     /locus_tag="ACH3L1_000911"
     CDS             complement(12552..13046)
                     /locus_tag="ACH3L1_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFT4075757.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13237..14121
                     /gene="yghA"
                     /locus_tag="ACH3L1_000912"
     CDS             13237..14121
                     /gene="yghA"
                     /locus_tag="ACH3L1_000912"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFT4075758.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14393..14818)
                     /gene="exbD"
                     /locus_tag="ACH3L1_000913"
     CDS             complement(14393..14818)
                     /gene="exbD"
                     /locus_tag="ACH3L1_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFT4075759.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14825..15559)
                     /gene="exbB"
                     /locus_tag="ACH3L1_000914"
     CDS             complement(14825..15559)
                     /gene="exbB"
                     /locus_tag="ACH3L1_000914"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFT4075760.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15811..16998
                     /gene="metC"
                     /locus_tag="ACH3L1_000915"
     CDS             15811..16998
                     /gene="metC"
                     /locus_tag="ACH3L1_000915"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFT4075761.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17138..17797
                     /gene="yghB"
                     /locus_tag="ACH3L1_000916"
     CDS             17138..17797
                     /gene="yghB"
                     /locus_tag="ACH3L1_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFT4075762.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17837..18736)
                     /gene="yqhC"
                     /locus_tag="ACH3L1_000917"
     CDS             complement(17837..18736)
                     /gene="yqhC"
                     /locus_tag="ACH3L1_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFT4075763.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18930..20093
                     /gene="yqhD"
                     /locus_tag="ACH3L1_000918"
     CDS             18930..20093
                     /gene="yqhD"
                     /locus_tag="ACH3L1_000918"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFT4075764.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20198..21025
                     /gene="dkgA"
                     /locus_tag="ACH3L1_000919"
     CDS             20198..21025
                     /gene="dkgA"
                     /locus_tag="ACH3L1_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFT4075765.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            21225..22151
                     /locus_tag="ACH3L1_000920"
     CDS             21225..22151
                     /locus_tag="ACH3L1_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFT4075766.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22202..22459
                     /gene="yqhH"
                     /locus_tag="ACH3L1_000921"
     CDS             22202..22459
                     /gene="yqhH"
                     /locus_tag="ACH3L1_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFT4075767.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22502..24721)
                     /locus_tag="ACH3L1_000922"
     CDS             complement(22502..24721)
                     /locus_tag="ACH3L1_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFT4075768.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24832..26244)
                     /gene="ftsP"
                     /locus_tag="ACH3L1_000923"
     CDS             complement(24832..26244)
                     /gene="ftsP"
                     /locus_tag="ACH3L1_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFT4075769.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26319..27056)
                     /gene="plsC"
                     /locus_tag="ACH3L1_000924"
                     /gene_synonym="parF"
     CDS             complement(26319..27056)
                     /gene="plsC"
                     /locus_tag="ACH3L1_000924"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFT4075770.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27290..29548)
                     /gene="parC"
                     /locus_tag="ACH3L1_000925"
     CDS             complement(27290..29548)
                     /gene="parC"
                     /locus_tag="ACH3L1_000925"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFT4075771.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(30094..30576)
                     /locus_tag="ACH3L1_000926"
     CDS             complement(30094..30576)
                     /locus_tag="ACH3L1_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFT4075772.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30629..31021)
                     /gene="ygiW"
                     /locus_tag="ACH3L1_000927"
     CDS             complement(30629..31021)
                     /gene="ygiW"
                     /locus_tag="ACH3L1_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFT4075773.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            31173..31832
                     /gene="qseB"
                     /locus_tag="ACH3L1_000928"
     CDS             31173..31832
                     /gene="qseB"
                     /locus_tag="ACH3L1_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFT4075774.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31829..33103
                     /gene="qseC"
                     /locus_tag="ACH3L1_000929"
     CDS             31829..33103
                     /gene="qseC"
                     /locus_tag="ACH3L1_000929"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFT4075775.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(33149..33481)
                     /locus_tag="ACH3L1_000930"
     CDS             complement(33149..33481)
                     /locus_tag="ACH3L1_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFT4075776.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            33800..34381
                     /gene="mdaB"
                     /locus_tag="ACH3L1_000931"
     CDS             33800..34381
                     /gene="mdaB"
                     /locus_tag="ACH3L1_000931"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFT4075777.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34412..34726
                     /locus_tag="ACH3L1_000932"
     CDS             34412..34726
                     /locus_tag="ACH3L1_000932"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFT4075778.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(34774..36666)
                     /gene="parE"
                     /locus_tag="ACH3L1_000933"
     CDS             complement(34774..36666)
                     /gene="parE"
                     /locus_tag="ACH3L1_000933"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFT4075779.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(36695..37276)
                     /gene="yqiA"
                     /locus_tag="ACH3L1_000934"
     CDS             complement(36695..37276)
                     /gene="yqiA"
                     /locus_tag="ACH3L1_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFT4075780.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(37276..38103)
                     /gene="cpdA"
                     /locus_tag="ACH3L1_000935"
     CDS             complement(37276..38103)
                     /gene="cpdA"
                     /locus_tag="ACH3L1_000935"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFT4075781.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(38128..38550)
                     /locus_tag="ACH3L1_000936"
     CDS             complement(38128..38550)
                     /locus_tag="ACH3L1_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFT4075782.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(38551..39180)
                     /gene="nudF"
                     /locus_tag="ACH3L1_000937"
     CDS             complement(38551..39180)
                     /gene="nudF"
                     /locus_tag="ACH3L1_000937"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFT4075783.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            39420..40901
                     /gene="tolC"
                     /locus_tag="ACH3L1_000938"
     CDS             39420..40901
                     /gene="tolC"
                     /locus_tag="ACH3L1_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFT4075784.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            41049..41720
                     /locus_tag="ACH3L1_000939"
     CDS             41049..41720
                     /locus_tag="ACH3L1_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFT4075785.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            41726..42886
                     /locus_tag="ACH3L1_000940"
     CDS             41726..42886
                     /locus_tag="ACH3L1_000940"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFT4075786.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(42924..43712)
                     /gene="ygiD"
                     /locus_tag="ACH3L1_000941"
     CDS             complement(42924..43712)
                     /gene="ygiD"
                     /locus_tag="ACH3L1_000941"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFT4075787.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43855..44628
                     /gene="zupT"
                     /locus_tag="ACH3L1_000942"
     CDS             43855..44628
                     /gene="zupT"
                     /locus_tag="ACH3L1_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFT4075788.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(44686..44856)
                     /gene="yqiD"
                     /locus_tag="ACH3L1_000943"
     CDS             complement(44686..44856)
                     /gene="yqiD"
                     /locus_tag="ACH3L1_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFT4075789.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(45117..45770)
                     /gene="ribB"
                     /locus_tag="ACH3L1_000944"
     CDS             complement(45117..45770)
                     /gene="ribB"
                     /locus_tag="ACH3L1_000944"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFT4075790.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45874..46022)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            46144..46434
                     /gene="ubiK"
                     /locus_tag="ACH3L1_000945"
     CDS             46144..46434
                     /gene="ubiK"
                     /locus_tag="ACH3L1_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFT4075791.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11561 a        12419 c        11585 g        10931 t
ORIGIN      
        1 aatatattca catatgaaat gaataaatat tggaatatat aaatattgaa tattttgatt
       61 aatccccgta ctaattattc ttcataatca gtacggggtt gctacaacat gtattacttt
      121 ttcaccacaa ttaacggttc aatatcactc tcttttttga tgaccagtga ttcatcaccg
      181 cgcaaacacg tcccaccgta gttgccgcca acggtgaagg tacatacctg aatgtattta
      241 ccgtccactt tcggcaaaca ccacagttgc tgatagatgt ttttctgctc ggcaaattta
      301 ccgctggttt tgtccagcac ctcttcatga tggctgacca ggtcgatatt gctaccacag
      361 cgaccggcga tcggtttcac tgcgtaacct gttttcacca attcatcatt aacagtgaaa
      421 tcggtatcca gcaggtaacg atggtgcggg aacagcgacc agaggatcgg cagaatcgct
      481 ttgttgccgg ggatcaccgt ccacagcggc tcaaagacca gcacttccgg gcgcagcaat
      541 acgtcgataa gacgcacttc gttttgcgga tgaccggtac ggattggcac cgcagcaaac
      601 tcacggtcgc taacttcacg aatctgatca aacgcggttt cccacgccca ggttttccac
      661 acgcagttaa ccagtcgccc ttccccatca atcagttgcc cggcagcatc ccagcccagt
      721 tcatccagcc cgcgcaagat acgcgtttca aagcccgcct ggtgcagcgc ctgctccata
      781 aactgcgcgt gatagttttc ctcgatatct ttgtcctgca tgatatggac aaacggacgt
      841 gcacgactgt gtttccaggc accagccagt tcgttaatca gcccttccgc cggattgaag
      901 ccgttgcctt tatagccctg ctccgcccaa cgttcgagga tcaagcccgc ttcggtatga
      961 caggaggcgg agtcggcgtt gtattcgtaa accttcaggc cacgctcatc catgcagaaa
     1021 tccatacgac cagtgatcat atggtgacgg cgacgctgcc aggagagacg caaacgtggc
     1081 cagaggattt tcggaatgtc gaacagcgcc agcaggttgt catctttcag caccttgtcg
     1141 gttgcgtgaa gatacatcag atgcagctcg ttggtggctt taattagctc ctgctcggca
     1201 ctctcggtaa tggtgtagta atgatacgga tcctgattga tcacctgacc gttggcctgc
     1261 acataggcgt tttgtagcgg atctttttca tccagccatt taccgtcaaa ctggcctttg
     1321 ttttccagac gcgctccact gattttcagc agctcgcctg caatttccgg ctgcggtaag
     1381 ctgtattcag tatcttccgt ctggatcatc cagcccagaa tggtggtgtc atcaaaagtg
     1441 tctttaaggg tatagcagcc gttttcgacc accatctcca gctcgcgcgt ccactgttgc
     1501 ccttgcggca acggggaatg aatcacgttc tgttccgcaa tacggacttt gttgccatgc
     1561 aattgggtaa tgatggcgac atggccagtg tctttaaatt caccgccttt atcccagata
     1621 agaagcgcac ccgcgaccgg cgcacgcggc gagccgttag gaaatgcctg caatggcagg
     1681 atgttgtcat taaccacttc tcgcaggaag cgcagcgaga aaatctccca cgccataccc
     1741 acgtcagtaa agaccacacc gtaattcaga aagagaaaac ggcgagcaaa ttcaacgcat
     1801 tgccacttgt ggcccatata ttcgtcgtcg atatagctac ggaatacggc gtcatcttcg
     1861 tattcctgcg gatcgagaga actgtaatct gaagagtaga ttgctacccc acctggggcg
     1921 tagcccaata atgtcccgaa cggggcatcc tggctggtcg ttcctttgct catcacttta
     1981 cctttaacaa tacaacctaa gcagttggcg taattgttgt gctctgatta cctgctcagc
     2041 gacattaacc ggacagaggt cagactaatc atacacctca tcgcagcggc tggcgcaaag
     2101 ggttaaaaaa ttcacattct gtacagcaag tgacctgcta cactgcttca acactaccac
     2161 tcagaaggca actcactatg acagacaata cttatcagcc cgcgaaagtc tggacgtggg
     2221 ataaatccgc tggcggcgcg ttcgccaata tcaatcgccc gttttctggt ccgacgcatg
     2281 aaaaaacgct gcccgttggc aaacatccat tgcaacttta ttcgctggga acgccgaacg
     2341 gtcagaaagt aacgattatg cttgaggagc tactggcgct gggcgttact ggtgcagagt
     2401 acgacgcctg gctgattcgt attggcgatg gcgatcaatt ctccagcggc tttgtcgaag
     2461 tgaacccaaa ctcgaagatc ccggcgctgc gcgatcatac gcataatccg ccgatccgcg
     2521 tgtttgaatc tggttcgatc ctgctttatc tggcggagaa atttggctac ttcctgccgc
     2581 aggatctggc aaaacggact gaaacgttga actggctgtt ctggttacag ggtgccgcgc
     2641 cgttcctcgg cggtggtttt ggtcactttt accattacgc gccggtgaaa attgagtacg
     2701 ccatcaaccg ctttaccatg gaagccaagc gcctgctcga cgtgctggat aagcaactgg
     2761 cgcagcataa gtttgttgcg ggcgatgagt acaccattgc ggatatggcg atttggccgt
     2821 ggtttggcaa cgtggtgctc ggcggcgtgt atgatgccgc agagtttctg gatgcgggaa
     2881 gttataaaca tgtgcaacgc tgggcgaaag aagtgggcga acgtccggca gtgaaacgtg
     2941 ggcgtattgt taaccgcacc aacgggccgc tgaacgagca gttgcatgag cgccatgacg
     3001 ccagtgattt tgagacaaat acagaagata agcgtcaggg gtaagggttg gtgttcgtcg
     3061 cagcaagcca tccaggccgg ataaggcgtt cacgccgcat ccggcaatcg tgcacaatgc
     3121 ctgatgcgac gctgccgcgt cttatcaggc ctacaaaagc taatcatcac gcgctggtaa
     3181 tgtcgtaatc catttggcgc agtgcgtcta atgtggcttt ggcttcatct tcgtcgatga
     3241 tgctcatggc aaatccgacg tgtaccagca cccactggcc cagcagatcg gcagggttac
     3301 cttcgcaaat cagggcgata ttcacgtcgc gcttgatacc acatacttca acctgcgcaa
     3361 gctggtgaat atcttcaccg acagccagca cctggcctgg aacgccaata cacattgtta
     3421 actccggtta tttcgattgc cggacaaacg cgaaaaagaa cctttagcgg gtgttccctc
     3481 tcaccctaac cctctccccg gaggggcgag gggaccgtcc gtgcgctttt taggcgttgt
     3541 cctcagtctt attcaacttc aatacttttg acgatcagcg aatcgccggt atcgacacgc
     3601 aaccgctcgc cgtgacagag cggacactgc gcatcgtgct gatgaatctc caccacctgg
     3661 ctgcaatccc agcaccaggc ctgggcgggt ttatagacga tatgtaaatc gcacccttgc
     3721 gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga cggcgctctc ctcaacgcag
     3781 gagagcgcgc caatttccag ccacacggcg gtgacgcgct taacatcgtg ctgctccgcc
     3841 tgccgttgga taatttcaac ggcgctctgg caaagcgaca actcatgcat tttcgcccct
     3901 cctgcggcca aacagcaggg cgcgacgccc tgcgtgtggt acatccggat tggtgactgg
     3961 caacgaaagg atcattcgtg cgcagtcatc cgtcaggcgt tggccttctt caatcgacat
     4021 gctgtgcgaa agcggcgaca tcagcgaaca ggagaggtat tgcgaaacac cgtccagttc
     4081 gccaacggta aaggtcatgg tgccatacgg cagttgcaga ccaatttttt cactcacttt
     4141 gcgcagcggc cagagttgat ccgggccggg gaagataact gcactcagca tccacggggt
     4201 gatcacacac cccgtccact gaccttcgaa cagcgtaaaa tcagaaacat acaccggcat
     4261 tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg gcaatttctt caaacgctgc
     4321 ctgtacttgc gccttcgggg aggtctggaa acctgctatc tcttcagtca tgaatcgcct
     4381 cccgtgggat ggcttccacg cctgattcgc gcagcgcagc cagaacctgc tcaagcgcag
     4441 gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg tggctccagc gattccggga
     4501 tcacgccgac cagggtcagc tttttcggaa actcaccggt gaagcgcagg gccgacaata
     4561 cgtcggccag gccaagctga tgcggagaga ttttgttggt aaacaacgcc ggaacttctt
     4621 catcccgcag gatcatcatc gttcccggag tgttcttttt cgacacaatg gcatccgcaa
     4681 taatcagatg atcgcgattt gccatgtcgc caagcagctc cattcccgcc gtgccgccat
     4741 cgaggatctc aacataatcc ggcagaatgt atcgttgctc taacgcttcg acaatccgca
     4801 cgccgatggc ttcatcggtc agcaaaatat tgccgacccc taagactaaa atacgcatta
     4861 cagaaccttc actgaaacca cttcgttgcc gtcggcatcc actacgtgta ccgcacaggc
     4921 catgcacggg tcgaaggagt gaatggtacg caccacttcc agcggtttat tcggatcggc
     4981 aaccggtgta cccaccagcg actgctcgta aggaccgacg tcatcattga agttacgcgg
     5041 accagagttc caggttgatg gaacaaccgc ctggtagttg ctgatgatac cgtctttaat
     5101 aaccatccag tgagagagca taccgcgcgg cgcttcgagg aagccaacac ctttgaactc
     5161 acccgttgcc ggaatgttcg gcttcacaaa ggtggtgtga tcgcctttgc cgatattggt
     5221 gatcagtgca ctgtattggt tttgcaggat atcctgcaat tcacagcagt gaacggtacg
     5281 accaataata cggcccagcg tggagtgcag ctgtgccact tccagcgtgt tgccagtcag
     5341 tttctgataa atcgcaacga tttcattcag tttgttctgg gtagattcgc gacctgccgc
     5401 cagtttcacc agcatattag ccagcggacc cacttctacc gttttgccgt agaaagtcgg
     5461 tgatttcacc caggaatatt tcccgtcgtc agaccaacca tcataagccg gaatggtggt
     5521 accttcccac ggcgcctgtg gcgcttcgtc tttataccag gagtgcttcg cgctttcctg
     5581 aatgcctttg atcaggtatt catcggaatg agaagtgatc ggacgatacg aggacagatc
     5641 cgcattctca atgtagcctc ccgggaacag gaagctgccg tttttgctgt cggtcgggaa
     5701 ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc gtcagccatt ccgggtagaa
     5761 cgcggcgata accgcggtat caaccttata aacctgctca acaaagtcgc tcagtttgtc
     5821 gatgaaagac ttgatgtaca tcaggcgctc aaggttcaac acgcccaacc cgtcgaggtt
     5881 gattgggttc gcgacaccac ccacagccag gttctgaatg tgcggcgttt taccgcccag
     5941 cagcgccacg acgcggttag cgtcacgctg gcactccagc gcctgcaggt agtgcgctac
     6001 cgcaatcagg ttcacttccg gcggcagttt catcgccggg tgaccccagt agccattggc
     6061 gaaaataccc aactgaccgc tggcaaccag atctttgatc ttgttctgaa ctttagtgaa
     6121 ctcttccgga ctgttcagat gccaggtcga aacgcctttc agcatttcgg aggctttggt
     6181 tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc agcgccgaaa gctgatagaa
     6241 atgaacgata tggtcatgcg tggtgtgcgc agccagaatg atgttacgga tgtattgcgc
     6301 gttaaccgga acgtcgatat tcagcgcact ttctgccgca cgaacggaag acagcgcgtg
     6361 agtggtagta catacgccac agatacgttg cacaatcatc catgcatcgc gcggatcgcg
     6421 gtttttcacg atctcttcca tgccgcgcca catggtaccg gaagcccatg ctttcgaaac
     6481 gacgccattt tcgatttcgc aatcgatgcg taaatgcccc tcaatacggg ttaccggatc
     6541 aatagtaatt ctctggctca tgctttgctc gcctcatgac gattatgatc gttttgtttt
     6601 aaaggaggaa gtatcggcag tagacgaatg agtacgatgt aagcgcaaat ctcaatagcc
     6661 acaaaaccaa tagaaatcaa cagttcttcc caggtcggga agtaggcgta accgccgccc
     6721 gggttgaatg ccaccaacga ataggtcaga cgccaggttg cacaacctaa cagtgcgctc
     6781 agtgctgaca ggaacagcat acgggaatca ttacgcagct tcgccacacg cagaacgacc
     6841 agcgggaaga gcatcagcag gacttcaatc cagaacatca cggagtagaa gtcaccggca
     6901 aacgctaacg acagcttgtc gcgatagata agctcgccaa agcgcagccc gatgaaaatc
     6961 gccagcaaca cactgatggt gttggtcagc ttgacgaaca ggctcttttc atccggaccg
     7021 ttgccacgca gacccgcctg caccagtgaa ccttcaaaga tgacaatcga gaagcccatg
     7081 atgaacgccg tcagcagcga gaacagcggc aacatttcat agctctgcca gagcggatgc
     7141 accttgtagc ccgccgagat catcagcgac cccattgaag actggtgcat ggtcggcagc
     7201 agcgcaccga gcgcgatgat gaagaacatc accttgttca gacgttgtag cgacaccttc
     7261 cagcccagac gttcaaacag tgccggagca aactccagag ccatcacgcc gatgtagatg
     7321 gtcatacaga ccgccgtctc gaacagtacc gagttcacgt tgaagtgacc cggaatgtag
     7381 aagtacggca ggttccagta gcgacccacg tcgatagtga tcgacaagcc acccagtgag
     7441 taaccaaaca gactcgccaa cagcgccgga cgcaccagcg gatggtattg cccacggtta
     7501 aagacgtata ccgcccacgc cagcgcccag ccgccacagg caaagccggt gccaatcagc
     7561 aggtcaaacg cgatccacac gccccacggg aagccgccgt tcaggtcaga gacagagccc
     7621 agaccgaaca ccagacgctt cacaatcagg agcatacaga tgacgattaa cggtccaaaa
     7681 atcatgaccg gtttactgat gattttgccg cccagcggtt gtggatcatg actcatgatc
     7741 gtctcctccg tcgtgatggt cgtttttggt gttgcgacga accagcacgg ttaagcccgc
     7801 cagcacagcc agtggtagca tcatgccttt atacagggtg tgttgaatat tttcggaacg
     7861 cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga ttttcataag gcacacccgt
     7921 cagtaccaga acctgagtac cgccgccctc tttctcgccg tacagatgcg gataatattt
     7981 cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc tgacgcggat agtggtattc
     8041 gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc atcagctctt cacgcgtacc
     8101 gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca ggcagaccgc ctttatcgag
     8161 acgttccaca cctttctggt tgcacagctc gcacttatgc agcgcaccaa acgggttgtt
     8221 gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg cagtaacggc aaccggtgca
     8281 cacgtctttg tcgtaatgga caatgccggt tttcggatct tttttcagtg cagagaccgg
     8341 gcacacagag acacagttcg gatcgacgca gtgcatacac tgtttcttaa tgtacgcgta
     8401 gccgttctcc tcctggtctt tgttgacccc tgtgccgctg gtccacacct gaatgatgtt
     8461 attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc tgttcccctt gcgggttacg
     8521 ttcagggaag ttgatatcct gacacttggt gacgcaagcc tggcagccta cgcacaaggt
     8581 cgagtcgtac aacatcccca gcgatcccgg aattggcggg cggttttcag cagccgcatg
     8641 actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag gaggctgctt taataaaatt
     8701 acgtctgttc acggttattc tccccgtgag tcagcgttat ctttcttttg ctgacgaccc
     8761 agttcacgca ccgccatcac gctgacaccg gcaaccagcc caaccacacc gccgagcaaa
     8821 ccaatagcgc ctgcagagac gttgccgccc tctttagcgt taacatccgg tttctgtgaa
     8881 cgcggagtct gattttcgac gttggcaagc tgatggatgc ctttatggaa gccgatacct
     8941 ttttcgttac agccatagca cgggtgacca atcgccaccg gccacacacc gccaacatcg
     9001 cagaattgca gcgttgagca gttgccgtaa gtttctggcc ctttacagcc gaggtggtac
     9061 aagcaccagc cttcgcggtg gccttcatca ccgaactctt tggcaaaacg accagcatcg
     9121 aagtgcgggc gacgttcgca gtgttcgtga atcagacggc cataggcgaa ggtcggacgg
     9181 tttttgtcat ccagtttcgg cggtttgccg taagtgatga tgtgcgcaac ggtcgcgagg
     9241 aagttgtgcg ggttcggcgg gcagcccgga atattgataa cggttttgcc tggcagaact
     9301 tcttgcaagc tgactgcgcc agttgggtta actccagctg cggcaacacc gccccacgcg
     9361 gagcaggaac caatagcgat aatggctgct gagccttccg ccgctttgcg gatgtgatcc
     9421 acaatcggct caccggcaac catgcaataa ataccgttat cttttaatgg gatggaacca
     9481 tccaccacta acacatactg ccctttgtac ttctcgagag cgttatgttt gttctcttcg
     9541 acctgatgac cgaaggcggc ggaaagcact tcgtgatatt ccagagagat agtctccagc
     9601 acgaggtttt ctaccgttgg atgcgttgca cgaagcagag attccgtaca accggtgcac
     9661 tcctgcgcgc caatccagat aactggcgga cgctgcgggt tagtaaccga ttcggccatc
     9721 tctgcagcgg ctttgctact taaccccatg gtggcggcta atgctgcaca aagcttcatg
     9781 aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt tatctccagt catttatagt
     9841 tattccgttg cgagaacctg gcatatattt tgcctcaatc gcaaaatcaa taatgcgatc
     9901 gatgcgccat ttaccacaca tttattatgg ttatcgatat catgatactg cggcggggcg
     9961 ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag caagataata cgtatgtttg
    10021 atcaattttc gaggcgataa ccggccataa aaaagcgcaa cgtagaacag gaattattct
    10081 cacgaaagaa atccattacc atctctttat ccagtccata gcggcgcgtc agaatacccg
    10141 cttcccaggc gctaagctgg cgatctccgg tcttttcgta catcctgtgt gagtagccta
    10201 atacaaaacc gcgtttataa tcggcacaga attttgtcac attaaccgca ctgtcggcat
    10261 gcgttgcttt taatcccgcc attaagcctt taccaaaatg gttattcatc gttcacctca
    10321 ttcattgtat atcgccatat ataacgatat tttaggcaaa gaacgacttt tgcgcacgct
    10381 attttttcat acttgtttaa gacgtaatcc cgtaagagat aaacctatca ggagagatat
    10441 ttatcgaacc atttcagtgt cctttgccag gcaagatcgg cggcagattt gtcataacgg
    10501 ggcgtggaat cattatggaa tccgtgatta acccccggat agatataagc ctcataaacc
    10561 ttattattgt ctttcaacgc cgcctcgtaa ggaggccagc cctcgttgat tcgggtgtcc
    10621 agttccgcga agtggagtag taaaggcgct tcaatcttcg ccacatcggc agtgggtgcc
    10681 tgacgaccat aaaacggcac cgcgcaggcc agttccggat acgcgacagc cgccgcgttc
    10741 gatacgccac cgccatagca aaatccggta atacccactt tacctgtcgc ttgcggatag
    10801 cgttgcataa actcaattgc ggcaaagaaa tcattcatca gtttggttgg atcaacctgc
    10861 tgttgcagct cacgaccttt atcatcattt cccggataac ctccaacgga acttaatccg
    10921 tcaggtgcca gggcgatata ccccgccttc gccactcgcc gtgccacatc ttcgatatac
    10981 ggattcagtc cacgattctc atgcaccacc cccacggctg gcgttttgcc gctcattttt
    11041 gcgggtttca ccagataacc ccgtacctcg ccgtgaccat ttggcgaagg atacgtgatg
    11101 tactcagcaa caatctccgg gtcggtaaac tctacctgag tcgccagcgc ataatttggc
    11161 ttgagcaaat caaacaacgc taatgccgtc atcccgccca ccgcatactt cgccgcaagg
    11221 ttgaggaact cacgtttcga aattttcccg tgaacgtaat agtcgtagta atccaacaac
    11281 tcttgtggga aatctttggc ggttaaacgc ggcatcgttg cattcctcaa ctggtgtttt
    11341 tttaagcaaa gcataagcgc gtgtttttgc ccagtttttc gtcactctgt gagccagact
    11401 acgggatacg cgctggcgaa tcgctaaact agaaacattg tttcgaaatt gaacggtgga
    11461 aaggagaggt catggtctgg ttagcgaatc ccgaacgtta cgggcagatg cagtaccgct
    11521 attgcggaaa aagtggttta cgcctgcccg cattatcgct cggtttatgg cacaatttcg
    11581 gtcacgttaa cgcgctggaa tcacagcgtg caatcctgcg taaagcgttt gatttaggca
    11641 ttacgcactt tgatttagcc aacaattacg ggccgcctcc aggaagcgca gaagagaact
    11701 ttggtcgcct gctgcgcgag gattttgctg cttatcgcga tgaactgatt atctctacca
    11761 aggctggcta cgatatgtgg cccggcccgt acggctctgg cggttcacgt aaatacctgc
    11821 tcgccagcct cgaccaaagc ctgaagcgta tggggcttga gtatgtcgat atcttttact
    11881 ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc ctctgcgctg gctcatgcgg
    11941 tacaaagcgg taaggcgctg tatgtcggga tctcctctta ctcgccagag cggacgcaaa
    12001 aaatggtcga gttgctgcac gagtggaaaa ttccgctttt aattcatcaa ccttcgtaca
    12061 atttactgaa ccgctgggtg gataaaagcg gcctgctgga taccctgcaa aataacggcg
    12121 tgggctgtat tgcctttact cctctggctc agggattgct gaccggaaaa tatctcaacg
    12181 gcattccgca agattcacgg atgcatcgtg aagggaataa agttcgtggt ctgacgccga
    12241 aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt gaatgaaatg gcacagcagc
    12301 gtggacaatc aatggcgcaa atggcgttaa gctggttgct gaaagatgat cgcgtgacgt
    12361 cggtattgat tggtgccagc cgcgcggagc aactagagga gaacgtgcag gcgctgaata
    12421 atctgacatt tagcaccgag gagctggcgc agattgatca gcatatcgcc gatggcgagc
    12481 tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa taacgggcgc gcgaaccgcg
    12541 cccgaatcag atcagtgatt atgacgagtc agtcggtcaa gatagcccat cacaaatgca
    12601 gaaagcacaa acgtcagatg gataatgacg taccacatca gtttgttatc cgggacattt
    12661 ttcgcatcca taaagacgcg cagtaagtga atggaagaaa ttgccacaat cgacgctgct
    12721 actttgtttt tcagcgacgt tgcgtccatt ttccccagcc acttcagctt ctctttgttc
    12781 tcggagatat ccagctgtga gacgaaattc tcataaccgg aaaacatcac catcaccagc
    12841 aaaccgccaa ccagcgtcat atccaccagc gacagcaaca cgaggatcaa atctgattcc
    12901 gccatcgaga agatattcgg cagtacgtga ataatctcct ggaagaactt cagcgccagg
    12961 gcaactaacg ccagcgaaag gccaaagtac acgggggcaa gcagccagcg agaagcatac
    13021 attgcatttt caagaaaacg ttccatacat tcctgtcgtg tagttaaaac gggcaacagt
    13081 atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac atatacgtta aacctttgtc
    13141 gcataattct gaggtttagg gatcttcctg gttcccttat caacgcatcg agagcgtcta
    13201 cccttaaagg acgttgaacc tgaagggaga aaaacgatgt ctcatttaaa agacccgacc
    13261 acgcagtatt acactggtga atatcccaaa cagaaacaac cgacgccagg catccaggcg
    13321 aagatgacac cgataccgga ttgcggcgag aaaacctatg ttggtagcgg tcgcctgaaa
    13381 gatcgtaaag cactggtgac agggggcgat tccggaatag gtcgcgctgc cgccatcgct
    13441 tacgcgcgtg aaggggctga cgtggcgatc agttatcttc ccgtggaaga agaagacgct
    13501 caggatgtga aaaagatcat tgaagaatgc ggacgcaaag ccgttctgct gccaggcgat
    13561 ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag cgcacaaggc gttaggcggg
    13621 ctggatatta tggcgctggt cgccgggaaa caggttgcca ttccggatat tgcagacctc
    13681 accagcgaac agtttcaaaa gacctttgcc attaacgttt tcgcgctgtt ctggctaacc
    13741 caggaagcga tccccctgct accgaaaggt gcaagtgtca tcaccacttc gtcaatccag
    13801 gcataccagc caagtccgca tttactggac tatgcggcta cgaaggcggc gattctgaac
    13861 tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta ttcgggtgaa tattgtcgcg
    13921 ccaggcccta tctggacggc actgcaaatt tccggcggac aaacgcagga taagatcccg
    13981 cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac cggcggaact ggcccctgta
    14041 tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg cagaagtgca cggcgtgtgc
    14101 ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa aagcaaccgg gcatcattgt
    14161 gaattcactc ttacctgatg tggcgaaaac gccttatccg cctacaaacg catgaagatt
    14221 caacatgttg caatagattt tgtaggcctg ataagcgtag cgcatcaggc attgagcacc
    14281 taatgccgga tgcgtcgtga acgccttatc cggcctacaa acgcacgaag attcaacatg
    14341 ttgcaataga ttttgtaggc ctgatgagcg tagcgcatca ggcaattcta ctttacttcg
    14401 ctttggcggt ttcttcgccg accagaccta tcttcaggta acccgcctga tgcagcgtat
    14461 ccattacctt catcaacgtc tcgtaatcga cggttttgtc cgctcggaag aagatggtgg
    14521 tgtctttctt gccttcggtt aacgcattca acgccgtaat cattgtttca tcggtgaccg
    14581 gatcgttacc gataaacatc gagttgtctg ccttcaccga cagataaacc ggtttttccg
    14641 ggcgcggctg cggcgtgctg gtagaagcag gcaagttcac cttcacatct accgtcgcta
    14701 acggtgccgc caccataaag ataatcagca gaaccaacat cacgtcgata aacggcgtca
    14761 cgttgatatc atgcatttcg ccgttatcgt cgaggttttc gttaagatgc attgccatcg
    14821 gacattatcc tgcgcgtaat ttttgtgcga cacgaaccgg atgcgcagcg gcgctggctt
    14881 ccagatctag gtcacggctt tgcagcaaca atacctgcgc tgcaacatca cccagcatcg
    14941 ctttaaagcc gccaatctgg cgtgcaaata cgttatagat aacgaccgca ggaatcgctg
    15001 ccacgaggcc gattgccgtt gctaacagag cttctgcgat acccggcgca acgactgcca
    15061 ggttagtggt ctgcgtttgc gcgataccaa taaagctgtt catgatgccc cagaccgtac
    15121 caaacagacc aacgaacgga gaaatcgcgc cgatggttgc cagatagccg ttaccgcggc
    15181 ccatttgacg acccactgcg gcgacccgac gctccagacg gaagctggta cgttctttaa
    15241 taccttcgtt atcgtcgctg ccttctgaca gttccagctc attctgcgct tcattgagca
    15301 aatgcaggct taagcttttg ctaccaaaat cagcggcgat atcgttggcc tggtttaagg
    15361 aacgtgcttc agccagcagt tgctgctcgc gcttaaggcg acgcttctga ttgaagaact
    15421 ctacgctctt actgaagaag attgcccagg tgactacaga ggccaaaata agcccaatca
    15481 tcacgcactt aacgacaata tcggcgtact gatacatacc ccagacggaa aggtccgtct
    15541 gcattaaatt attacccact gtgtatctcc aggacgcaag tcacaaaatc tgcgcataat
    15601 aatatcaaaa cgacgtcgaa ttgatagtcg ttctcattac tatttgcata ctgccgtacc
    15661 tttgctttct tttccttgcg tttacgcagt aaaaaagtca ccagcacgcc atttgcgaaa
    15721 attttctgct ttatgccaat tcttcaggat gcgcccgcga atattcatgc tagtttagac
    15781 atccagacgt ataaaaacag gaatcccgac atggcggaca aaaagcttga tactcaactg
    15841 gtgaatgcag gacgcagcaa aaaatacact cttggcgcgg taaatagcgt gattcagcgc
    15901 gcttcttcgc tggtatttga gagtatggaa gccaaaaagc acgcgacgcg caatcgcgcc
    15961 aatggcgagt tgttctatgg acggcgcgga acgttaaccc atttttcctt acaacaagcg
    16021 atgtgtgaac tggaaggtgg cgcaggctgc gcgctatttc cctgcggggc ggcagcggtt
    16081 gctaattcga ttcttgcttt tgtcgaacag ggcgatcatg tgttgatgac caacaccgcc
    16141 tatgaaccga gtcaggattt ctgtagcaaa atcctcagca aactgggcgt aacgacatcg
    16201 tggtttgatc cgctgattgg tgccgatatc gttaagcatc tgcagccaaa cactaaaatc
    16261 gtgtttctgg aatcgccggg ttccatcact atggaagtcc acgacgttcc ggcgattgtt
    16321 gccgccgtgc gcagtgtggt gccggatgcc atcattatga tcgacaacac ctgggcagcc
    16381 ggtgtgctgt ttaaggcgct ggattttggc atcgatgttt ctattcaggc cgccaccaaa
    16441 tatctggttg ggcattcaga tgcgatgatt ggcactgccg tgtgcaatgc ccgttgctgg
    16501 gagcagctac gggaaaatgc ctatctgatg ggccagatgg tcgatgccga taccgcctat
    16561 ataaccagcc gtggcctgcg cacattaggt gtgcgtttgc gtcaacatca tgaaagcagt
    16621 ctgaaagtgg ccgaatggct ggcagaacat ccgcaagttg cgcgagttaa ccaccctgcc
    16681 ctgcctggca gtaaaggaca cgaattctgg aaacgagact ttacaggcag cagcgggcta
    16741 ttttcctttg tgcttaagaa aaaactcaat gatgaagagc tggcgaacta tctggataac
    16801 ttcagtttat tcagcatggc ctactcgtgg ggcgggtatg aatcgttgat cctggcaaat
    16861 caaccagaac atatcgccgc cattcgccca caaggcgaga tcgattttag cgggaccttg
    16921 attcgcctgc atattggtct ggaagatgtc gacgatctga ttgccgatct ggacgccggt
    16981 tttgcacgga ttgtataaca ttgccacttt tggacaattt tgcagacatt ttattgtgaa
    17041 aagtcttaaa ttgttgcgtc cgggatcaag gcgtcccgga cgattcagga gtacaatagg
    17101 cagataaagg cttaaacgct gttccacagg aaagtccatg gctgttattc aagatatcat
    17161 cgctgcgctc tggcaacacg actttgccgc gctggcggat cctcatattg ttagcgttgt
    17221 ttactttgtc atgtttgcca cgctgttttt agaaaacggc ctgctgcccg cctcattttt
    17281 gccaggcgac agcttgttga tactggcagg cgcattgatt gcccaagggg ttatggattt
    17341 tctgcctacg attgcgattc tgaccgccgc agcaagtctg ggctgctggc taagttatat
    17401 tcaggggagc tggttaggga ataccaaaac ggtgaaaggc tggctggcac agcttcctgc
    17461 taaatatcac cagcgcgcca cctgcatgtt tgaccgccac ggtctgctgg cgctgctggc
    17521 tggacgtttt cttgcatttg tccgtacgct gctgccaacc atggcgggaa tttccggtct
    17581 gccaaaccgc cgcttccagt ttttcaactg gttaagtgga ttgctgtggg tcagcgtggt
    17641 aaccagtttt ggctatgcct taagtatgat tccgttcgtt aaacgccatg aagatcaggt
    17701 aatgacgttc ctgatgatcc tgccaattgc cttgttaacc gctggcttgt taggcacgct
    17761 gtttgtggtg attaaaaaaa aatactgtaa cgcctgacga ttttccccgt tcccggctgc
    17821 tgtaccggga acgtatttaa ttcccctgca tcgcccgcat tcttgccgca tcttcccccg
    17881 gcgtcacacc gaagtaacgt ttaaactcac ggctaaattg cgatgcgctt tcatagccga
    17941 cgcgcatcgc tgctgcgctg gccttcatgc cgtcatggat gatcatcatc cgcgccttat
    18001 gcagacggta attcttcaaa tactgcaacg gcgaggtgct ggtgacagac ttaaaattat
    18061 ggtggaacgc cgatacgctc atgttggctt ctgccgccag ttgctcgacg ctcaggtttt
    18121 cggtgtattt attctcaatt cgtttcagca cgcggctaat cagactgaag tgagtctggc
    18181 gactgaccag cgccagtaac gcgccgccgc aaggtccggt cagcacgtag tacagaattt
    18241 cgcggatgat ctgtttgccg agaatacgcg catccagtgg tcgctccatc acgtcgagta
    18301 accgctccgc cgcgcataaa atctcttctg ataacgtggc ggagttaatc ccgctggctg
    18361 ccatcgacgg ctggaaatgc tcatcttcgc caatgtccat caacagttcc tgtaactgca
    18421 aaatatcgac attgagacgc aaccctgcca gcggcacctc tgacgtggca taggtttcgc
    18481 actcaaacgg caacggcacc gtcagcagca ggtattcatt ggcatcataa cgaaacacgc
    18541 gttcattgat ataaccgatt ttatgcccgg aaaagagaat tatgatgcca ggctcgtaca
    18601 tcaccggtgt acgtgcgaaa ggcgtctcgc catacaacaa acgcacatcg ggcaacagtt
    18661 ctgacaaact attttcttta tttttcagtt tattaacttt atccgccagc aagcgacaaa
    18721 tctcttcacg tttcatatcg cgtaatttct taggaataat gcggcaattt gattgtgcgc
    18781 aattttgtag catttctcca gcactctgga gaaataggca agacattggc agaaatgagc
    18841 attgagagcc agggcgctgg cgatcacaat gaaaaacatc aggcagatcg ttctctgccc
    18901 tcatattggc ccagcaaagg gagcaagtaa tgaacaactt taatctgcac accccaaccc
    18961 gcattctgtt tggtaaaggc gcaatcgctg gtttacgcga acaaattcct cacgatgctc
    19021 gcgtattgat tacctacggt ggcggcagcg tgaaaaaaac cggcgttctc gatcaagttc
    19081 tggatgccct gaaaggcatg gacgtgctgg aatttggcgg tattgagcca aacccggctt
    19141 atgaaacgct gatgaacgcc gtgaaactgg ttcgcgaaca gaaagtgact ttcctgctgg
    19201 cggttggcgg cggttctgta ctggacggca ccaaatttat cgccgcagcg gctaactatc
    19261 cggaaaatat cgatccgtgg cacattctgc aaacgggcgg taaagagatt aaaagcgcca
    19321 tcccgatggg ctgtgtgctg acgctgccag caaccggttc agaatccaac gcaggcgcgg
    19381 tgatctcccg taaaaccaca ggcgacaagc aggcgttcca ttctgcacat gttcagccgg
    19441 tatttgccgt gctcgatccg gtttatacct acaccctgcc gccgcgtcag gtggctaacg
    19501 gcgtagtgga cgcctttgta cacaccgtgg aacagtatgt taccaaaccg gttgatgcca
    19561 aaattcagga ccgtttcgca gaaggcattt tgctgacgct aatcgaagat ggtccgaaag
    19621 ccctgaaaga gccagaaaac tacgatgtgc gcgccaacgt catgtgggcg gcgactcagg
    19681 cgctgaacgg tttgattggc actggcgtac cgcaggactg ggcaacgcat atgctgggcc
    19741 acgaactgac tgcgatgcac ggtctggatc acgcgcaaac actggctatc gtcctgcctg
    19801 cactgtggaa tgaaaaacgc gagaccaagc gcgctaagct gctgcaatat gctgaacgcg
    19861 tctggaacat cactgaaggt tccgacgatg agcgtattga cgccgcgatt gccgcaaccc
    19921 gcaatttctt tgagcaatta ggcgtgccga cccacctctc cgactacggt ctggacggca
    19981 gctccatccc ggctttgctg aaaaaactgg aagagcacgg catgacccaa ctgggcgaaa
    20041 atcatgacat tacgttggat gtcagccgcc gtatatacga agccgcccgc taagcttttt
    20101 acgcctcaaa ctttcgtttt cgggcatttc gtccagactt aagttcacaa cacctcaccg
    20161 gagcctgctc cggtgagttc atataaagga ggaacgtatg gctaatccaa ccgttattaa
    20221 gctacaggat ggcaatgtca tgccccagct gggactgggc gtctggcaag caagtaatga
    20281 ggaagtaatc accgccattc aaaaagcgtt agaagtgggt tatcgctcga ttgataccgc
    20341 cgcggcctac aagaacgaag aaggtgtcgg caaagccctg aaaaatgcct cagtcaacag
    20401 agaagaactg ttcatcacca ctaagctgtg gaacgacgac cacaagcgcc cccgcgaagc
    20461 cctgctcgac agcctgaaaa aactccagct tgattatatc gacctctact taatgcactg
    20521 gcccgttccc gctatcgacc attatgtcga agcatggaaa ggcatgatcg aattgcaaaa
    20581 agagggatta atcaaaagca tcggcgtgtg caacttccag atccatcacc tgcaacgcct
    20641 gattgatgaa actggcgtga cgcctgtgat aaaccagatc gaacttcatc cgctgatgca
    20701 acaacgccag ctacacgctt ggaacgcgac acacaaaatc cagaccgaat cctggagccc
    20761 attagcgcaa ggagggaaag gcgttttcga tcagaaagtc attcgcgatc tggcagataa
    20821 atacggcaaa accccggcgc agattgttat ccgctggcat ctggatagcg gcctggtggt
    20881 gatcccgaaa tcggtcacac cttcacgtat tgccgaaaac tttgatgtct gggatttccg
    20941 tctcgacaaa gacgaactcg gcaaaattgc aaaactcgat cagggcaagc gtctcggtcc
    21001 cgatcctgac cagttcggcg gctaacgtgc aaattctccc ggtggcggta atgttccgct
    21061 accggacttt tcagaaatca tttattcccc tcgcgtcccg cccgttgtta ctcttccttg
    21121 ttcaggaatg ccaaatataa ggacatcatc atgcagagcc ggaagctctt aaaagaacaa
    21181 ctcatctata tccgggataa acgcaacgga gaggtgaaaa acagatgaaa ataatacttc
    21241 tgtttttagc agccctggca agttttaccg tacacgcgca gcctccttca cagaccgtag
    21301 aacaaacagt ccggcagatt tatcagaact ataaatcaga tgccagtacg ccttatttcg
    21361 gtgaaaccgg agagcgggcg ataacttcag cgcgtattca acaggcgctt accctgaacg
    21421 acaatcttac actaccgggc aatattggct ggctggatta tgatccggtt tgtgattgtc
    21481 aggattttgg cgatctggtg ttagaaagcg tagcgataac ccaaactgac gtcgatcatg
    21541 ccgatgccgt tgtgcgcttt cgtatcttta aagatgataa agaaaagacc acacagacac
    21601 tgaaaatggt ggcggaaaat ggtcgttggg tcattgatga tattgtcagc aatcatggca
    21661 gcgtcttaca agcagttaat agcgagaatg aaaaaacgct ggccgcttta gcttcgttgc
    21721 agaaagaaca gccagaatcc tttgttgctg aactctttga acatattgct gattatagct
    21781 ggccgtggac gtgggtggtt tccgactctt accgccaggc ggttaatgcc ttctataaaa
    21841 ccaccttcaa gacggccaat aatcccgatg aagatatgca aatagaacgg caatttattt
    21901 acgacaatcc gatctgtttt ggcgaagagt cgctattttc acgcgttgat gaaattcgag
    21961 tcctggagaa aaccgccgat tccgcccgca ttcatgttcg ttttacgctg accaatggca
    22021 acaatgaaga gcaagagctg gttttacagc ggcgcgaagg taagtgggaa atcgctgatt
    22081 ttatccgccc gaacagcggc agcctactta agcagattga ggcaaaaacc gccgccagat
    22141 taaagcaatg agctgaatta aataacaatt agccggaaca ataaataaaa gggaacacta
    22201 tatgaaaacg attttcaccg tgggagctgt tgttctggca acctgcttgc tcagtggctg
    22261 cgtcaatgag caaaaggtca atcaactggc gagcaatgta caaacattaa atgccaaaat
    22321 cgcccggctt gagcaggata tgaaagcact acgcccacaa atctatgctg ccaaatccga
    22381 agctaacaga gccaatacgc gtcttgatgc acaggactat tttgattgcc tgcgctgctt
    22441 gcgtatgtac gcagaatgat aaaaaaatcc ccggcagcat gtcagttgcc ggggattttt
    22501 tttaacgtcc aaccgccgct ttagggcgtt tcttcgcacc agcattcacc ggacgagatt
    22561 gcgtagacga cgcttttttt gccgtagcag gcgtctgacg ctgagtcgcc atcggcgtat
    22621 gtttagtcaa cgccggacgg gtattgcggt tctggcgacg agcttcacgc atctcttcaa
    22681 tggttggcgc aggcactaag caatcgcgac ggctgccaat cagatgcttt ttgcccatcg
    22741 cttccagcgc ctggcggatt aacggccagt ttgccggatc gtggtaacgc aacaacgctt
    22801 tatgcaaacg acgctgtttg tcgcccttcg gtacgaagac gccttcgctc ttataaccaa
    22861 tcttcgccag cggttttttc ccggtgtaat acatggtggt tgagttcgcc agcggcgatg
    22921 ggtagaagtt ctgtacctgg tcgagacgga aacgatgctt tttcagccac agcgccaaat
    22981 tcaccatatc ttcatcacgc gtaccggggt gcgcagagat gaaatacggg atcagatact
    23041 gctctttacc tgcctgtttc gagtaggtat cgaacagctc tttaaagcgg tcatagctgc
    23101 ccatgcccgg cttcatcatc ttcgacaacg gcccttcttc ggtatgttcc ggagcaatct
    23161 tcagataacc gccgacgtga tgggtcgcca actctttgat atagcgcgga tcttccacgg
    23221 cgatgtcata acgtacgcct gaggcgataa ggattttttt aatgcctttc agatcacgcg
    23281 cacggcgata gaggttgatc gtcggttcgt ggttagtgtc catgtgcgga caaatatccg
    23341 gataaacgca cgacaaacgg cgacaagttt gttcagcgcg tggcgatttg cagcgcaaca
    23401 tatacatgtt ggcagttggc ccaccgagat cggaaatcac gcccgtaaaa cctggaacgg
    23461 tgtcgcggat cgcttcgatc tcattaatga tcgaatcttc cgaacggctc tgaataatgc
    23521 gcccttcgtg ctcggtaata gaacagaaag aacagccgcc aaagcagccg cgcataatgt
    23581 tgaccgaaaa acggatcatt tcgtaagccg gaatacgggc attgccatag gccggatgtg
    23641 gcacgcgctt gtacggcagc gcaaaaacgc tgtccatctc ttcggtagaa agcgggatag
    23701 caggcgggtt gatccacaca tagcggtcac cgtgtttttg catcaatgcg cgggcgcagc
    23761 ctgggttggt ttcgtggtgc agaatgcgcg aagcatgggc gtacagcact ttatcgccct
    23821 tcactttctc gaaagaaggc agcaatacgt aggttttttc ccacggtttc tggcgcggtg
    23881 gctgcacgat tacagctttc gcttcctgct ttttcggcgc aacaggtttg ttatccgcac
    23941 acggcaaatc ttcgccatac ggatgcggga ttgggtcgat tttccctagg gtatcaagac
    24001 gggtggaatc cacaccgctc cagccaggca gcgcctcttt tacgataatt gcggtattac
    24061 gcacatcgcg gatttcacta atcggctcgc ccatcgccag acgatgcgct acttccacca
    24121 gcggacgttc accgttacca aacatcagca tgtcggcttt cgaatctacc agaacggaac
    24181 ggcgcacggt atcggaccag taatcataat gcgcggtacg gcgcaggcta gcctcaatac
    24241 cgccgaggat caccggcaca tctttccacg cctctttaca acgctgggta taaaccagcg
    24301 tggcgcgatc cggtcgctta cccgcgacat tatctggcgt gtaggcatca tcatgacgta
    24361 aacggcgatc ggcggtatag cggttgatca tcgaatccat gttgccagca gtaacaccaa
    24421 aaaacagatt cggtttaccc agacgcataa agtcgtcttt gctgctccag tccggctggg
    24481 cgatgatccc gacgcgaaag ccctgcgctt ccagcatacg accgcaaatc gccatcccga
    24541 agcttgggtg atcgacatac gcgtcgccag taaccaaaat gatgtcgcag ctatcccagc
    24601 caagttgatc catctcttca cgagacatcg gcaaaaacgg tgccggtcca aaacaggcgg
    24661 cccagtactg cggccaggag aacaggtcgc gatccggttg gatcagggag atagagctca
    24721 ttttgcttcc agaaatgata aaaaaataat caaaggccgg ggattataag ccggaacgaa
    24781 agagaaatcg aaaggtattc catactcgcc ctcctcgggc gagtatgaag attacggtac
    24841 cggattgacc aacagttgcc caatcgagcc acggtccgcc atttccagcg tctgactgtt
    24901 gaagtagaac gggaagtgcg cccaggaagg ctgaccgaaa taaacaagca gctccacttg
    24961 tccgtcaacc caaacggtat ctttccagcc tctgtcttcc gggaacggca tcgcgccatt
    25021 cacgttacgg atctggaaca ttacgccttc aatatggaac gcttgcggct cgtccgcgcg
    25081 taccgtccag cgttcccacg ttccttgctg cgcggtgaca tcaatacggt tgacgtccca
    25141 cagctgtcca ttaatacccg ggtcatcacc cagactgata tcgcgactgc gaattggcga
    25201 accagccatg atttcagttg gcagcaagcg catcggaaga ctgtctgtga ccagcggcag
    25261 aaggccggtt gggcgcagcg ttagcaccaa ggtagaaacc agaatactgg atggctcaaa
    25321 gaagccgcga atacgatcaa caatgctcgc cgcttcacca caggtgatcg acacttcatc
    25381 gccgttgctc atatccacca gaatctcgcg gcgctcgccc ggtgccagcg aaagttgctt
    25441 caccgataca ggagcaggca ggaatccctg atcgccagaa atcacatgta acgggcgacc
    25501 atcgctcatc tgtagttgat agcgacgaga gttcgacgcg ttcagcagtc gcaagcgcac
    25561 ccagccacgc gagacttcaa cgtacgggct ttgtacaccg ttaaccagca gcgtatcacc
    25621 aacaaagccg ccgcttcccg gttcgttgta ttctggcgta ccaaagttat ccagccgttt
    25681 atcctggata atgaccggaa aatcatccac accataatgg ttggggatag gcagcgactt
    25741 gctgacttca tcttccacca gccacattcc ggcaaggccg ttatagacct gctgagccgt
    25801 gcggttggga gtattggcgt gataccacag agtagctgcg ttctggcgaa taggcagtac
    25861 gggtgcccag tcagcgtttg gcgacatcat ccgtgccgga ccgcccatca gcgggcctgg
    25921 tacctgtagc ccggccaccg tcattgagac attttctgtc aggcggttgc tgtaaataag
    25981 cttaacatcg tcgcccttcc agacgcggat agtcggcccc aggtaacgac cattgattcc
    26041 ccagaccgac gcgcgtgtcc ctggcgtaaa tgaccagtgc gcacgttgta cagtcataaa
    26101 cagcggttgc ccacggcgag attcaagtag cggcggaacg ggtagcggtt gctgttgccc
    26161 ggctgcgctg gccttcaggg gaacagcgcc tgcacaaagt gcaatccccg atgcctgaat
    26221 gaactgacgc cgactgagtg acatatttgc tccatgtaaa actggctaaa gtaataccgt
    26281 gcaggaaagt gttccccgca aaaatacaga ttgccgactt aaacttttcc ggcggcttcg
    26341 cgttctgcga cttctttatc gagctcggcg attttttgtt ccattatcga acgacaatgg
    26401 gcagccagct cacgaacctg atctttgcca tactgactga cgtcaattgg cggcagcatt
    26461 tcgacaatca ccagaccgtt gtgcagtcga ttaagattaa tcttattcga agttgtagag
    26521 acgcacacgg gaataatcgg gacgcccgcc gcaattgccg cgtgaaatgc tccagtcttg
    26581 aacggtagca ggccgcgacc acggctgcgg gttccttccg ggaacatcca gatggaaata
    26641 cggcgttttt tgaaatgatt cacgacttcc gcaatggtgc cgtgagcttt agtgcgattg
    26701 tttctgtcga tcaataagtt gccggttaac cagtacaact gcccgaagaa ggggatccac
    26761 agcaagctct ttttacctac cgtcaccgtc ggcggttgca caatgttcga tgctgtcacc
    26821 atgtcatagt tgttctggtg gttagcgata tagatagcat tgccgtagct ttcagcatct
    26881 gcaggtttgc ggcactcaac tttcaggccg aacagtggcg caagacggcc aaacatatgc
    26941 ccaaaggtgg ccacatgttt cgggttacgc gggctgaaaa ggcagtaaat ggagccgaat
    27001 acacagacta agatgctgta aatcacggta ataataagac gaaagatata tagcataaca
    27061 ccctctgaag gtctgagaga ctggcattgt acgtcacctg aaatcaggtt agggcattgt
    27121 taaaagccgc tctctggaaa agcgtattgt taatcgcaac gcatgaataa acaacggttt
    27181 caagggaatt tttatcgttt ccgggggttg aatacgctgc cggatgacga cttaacgttt
    27241 catccggcgt tccttgctag cgggaggaaa cagcgccctc cccggcatat tactcttcgc
    27301 tatcaccgct gctggcacgg cgaggagagt cgatctcaac acgatcgata cgctgcaaac
    27361 cgcgcatcaa cgtaccgcgg cgtccacgtt cgccagtgac tttctgtagc tcttccggac
    27421 gcagtttaat tttgcgtttc ccaacatgaa tggtcagcgt gctttgcggc ggcagaacgt
    27481 acagttgcgc cagaccatcc tcgccacgcg cggcttctgc cgatggaatg ttgataatct
    27541 tgttgccttt gcccttcgac agctgcggca gatcgcttac cgggaacatc aacatacggc
    27601 ctgcctgagt gattgccagc agcatatcgg aagcatcttc aatcaccacc ggcggcataa
    27661 catgggcatt ttccggtaag gtgatcaaag ccttacccgc acggttacgc gctaccagat
    27721 cgttaaaggt gcagacgaaa ccgtaacccg catcggaagc catcagcagt ttctgatcgt
    27781 cgctttccat cagcatatgg tcaacggtcg ccccaggcgg caacgttaat ttgccggtga
    27841 gtggttcgcc ctgaccacgc gccgacggca gcgtaatcgg gtcgatggca tagctacgac
    27901 cggtggaatc aacaaacact accggttggt tactcttacc tttcaccgcc gctttgaagc
    27961 tatcacccgc tttataattc aggcccggcg cgtcgatatc atggccttta gcgctgcgta
    28021 cccagcccat ctgcgacagc acaatggtga caggttcaga cggcagcatg tcgtgctcgc
    28081 tcatcgcttt cgcttcttcg cgttcctgca acggcgaacg acgatcgtca ccgtaggctt
    28141 gcgcgtctgc ctgcagttct ttcttcagca ggttattcat tttacgctcg gaagccaaaa
    28201 tgccctgcaa ctggtcgcgc tctttttcca gctcactctg ctcaccgcga atcttcatct
    28261 cttccagttt ggcaagatga cgcagtttca gttcgaggat cgcttccgcc tgggtttccg
    28321 taaggccaaa ccgcgacatc agcgccggtt tcggttcatc ttcgttacga atgatctcaa
    28381 tcacttcgtc gatgttgaga aacgccacca gcaaaccttc caggatatgc aggcgcttga
    28441 ggactttctc cagacgatag ttcagtcggc ggcgcacggt atcgcgacgg aacaccagcc
    28501 attcggagag gatttccagc aggtttttca ccgccggacg accatccaga ccgatcatat
    28561 tgaggttaat gcgatagctc ttttccagat cggtggtagc gaagaggtgg ttcatcacct
    28621 gatccatatc cacgcggttg gaacgcggca caatcaccag acgggtcggg ttctcgtggt
    28681 cagattcatc gcgcagatcg tcaaccatcg gcagcttttt gttgcgcatt tgcgcagcaa
    28741 tttgctccag tacgcgcgca cctgaaacct gatgcggtaa tgcgctgata accaccgcgc
    28801 catcttcttt cttccacacc gcgcgcatac gcactgaacc acgtccgttc tcgtagattt
    28861 tacgaatctc ggcgcgcgaa gtgataattt ccgcttcagt cggataatcc ggcccctgca
    28921 cgatatccag cagctgatcg agcgtagttt tcggctggtc gattaatgcg attgccgcct
    28981 gagctacttc acgcaggtta tgcggtggaa tatcggtcgc catgccgacg gcaataccgg
    29041 tggtgccgtt aagcaaaatg tttggcagac gggcaggtag cattttcggc tcctgcaaag
    29101 tgccgtcgaa gtttggcacc cagtcagccg tcccctgccc cagctcgctc aatagcagct
    29161 cggaatattt cgacaaccgg gattcggtgt aacgcattgc cgcgaacgat ttcggatcgt
    29221 ccggcgcgcc ccagttcccc tgaccatcaa ccagcggata acggtaagag aacggctgcg
    29281 ccatcaggac catcgcttca taacaggcgc tatcgccgtg cggatggtat ttacccagta
    29341 cgtcaccgac ggtacgggcc gattttttaa atttggcgct ggcattcagg cccagttcag
    29401 acatcgcata cacaatgcgg cgctgaacag gtttcagacc atcaccaata aacggcaacg
    29461 cacggtccat gatcacgtac atggagtagt ttaagtaggc gttttccgta aattcatgta
    29521 gcgcaaggcg ctctgccata tcgctcatta attctgattc ctcaacttat tcgccagcct
    29581 tcaaaggggc aatattgccg gagatactac attatctgcc gcgtcgagtc acaggagatc
    29641 agcgtgaata gcgccgccgg acgtgctccg acggcgataa aggcatcaat gtgccttgat
    29701 atacaactct ttgctgtaga cttgatcggc atagagggca gcacaaggtt gcgcccatgc
    29761 cacaccactc ggcatggggg tccgctccgg gcgattcgag aagttgaggt tatgcgagtc
    29821 cagggaagcc taaagcatca aagtagtcga ctgtaagtat acggttcagc ccagtttagc
    29881 tgtgtctcca ccagtgcccc gcgcctcctg ccataagtgc ggcttgctca tggatatccc
    29941 cgagacggct tacggcgcac cggataataa atttcgcgta tacatggtat tccttaccgg
    30001 agaaattata agcccaacaa caatacgcct gtgacattgt tcgctcaaac ttatcgcgag
    30061 tgatttggct cacactccgg taaagattcc ccatcacgcc aacggcacat aaacatccgt
    30121 ttccagtcgc tgctctgtca ccccttcggc aagattggtg tagtgaaaca gaatcggtgc
    30181 tttacgcatt ttctcgccgc ttgcaggcag ccagtggcga ataatgcccc atatcgtgtg
    30241 tgaaatatcg tccagctcgc caacgtggcg ggccacggca taacgtccac cggtaagttc
    30301 accattgctc acaccataac gattatcggg aatcggttcg ctaacgctgc cgcagatatc
    30361 aaagcgaaac gcttccggtg cggtggtggc aggggcgtcc caggcgacgc caaaagtctg
    30421 gctttggtta acaggagaaa gccccgtctc tttacgccac atgataaatt tcgccacact
    30481 gtagttgagc aactcagggc tacagcggtg cggcaacatc gccacaggga ttgatgggaa
    30541 atcgataatg tttacatcca gtgtcaggtt tgtcattttg cctccctgcg aaattattcc
    30601 cgggaacgcg tcccgggagc ggtaacaatt acggatttac tttgcggatc tgtttgacgt
    30661 caatttcaac agaattccag tctttatcga cttcaccctg aatctcaacc gtatctttcg
    30721 gcgtcaccgt cacaccgttc cagcgtttgt gatcgatatc aacattgata gtaccgctgg
    30781 catctttaaa cacgtagaga tcgtcagaga tgcgttcaac gatattgccg cgcagggtta
    30841 cccaggtgtc gtcacgcagg gattttgcgc tttctacagt cgttacgctg ccgttcggcc
    30901 cctgaaatcc tccggcctga ctttgcgtta ccgatgggcc agaaaaaccg ccctgctctg
    30961 ctgccatcac cggtgcactg cacagggcca ttactgcgat tactgctgcg aattttttca
    31021 tgtttattac tccctttaat gtctgtttcc gagcatttaa caagatagtc cttaacaact
    31081 tcttaaggga aaaaaataaa atttaatgct gtacagagcg cgttacaaca cggtttactg
    31141 gcagcaaata cggttatcgc agggatgaaa aaatgcgaat tttactgata gaagatgaca
    31201 tgctgattgg tgacggcatc aaaactggcc ttagtaaaat gggttttagt gtcgactggt
    31261 ttacacaagg tcgtcaggga aaagaggcgc tttatagcgc accttatgat gcggtgatcc
    31321 tggatttaac cttaccaggc atggatggtc gcgatatttt gcgcgaatgg cgagagaaag
    31381 gtcagcgtga gccggtactg aacctgaccg cgcgtgatgc gttagcggaa cgtgtagaag
    31441 ggctgcgtct gggagctgac gattatctgt gtaaaccttt tgcgttgata gaagtcgccg
    31501 ccaggctgga ggctctgatg cgccgaacca acggccaggc cagcaacgag ctgcgccacg
    31561 gtaacgtcat gctcgacccc ggcaaacgta tcgccacgct ggctggcgaa cccttaacac
    31621 tgaaaccaaa agaatttgcc ctgctggaat tactgatgcg taacgctggt cgggtactgc
    31681 cgcgcaaact gattgaagag aaactgtata cctgggacga agaggtcacc agtaatgccg
    31741 ttgaagtgca tgtgcatcat ctgcgacgca aactcggtag tgattttatt cgtaccgtgc
    31801 atggtattgg ctacacatta ggtgagaaat gaaatttacc caacgtctta gtctgcgcgt
    31861 caggctgacg ctaatctttt taattctggc ctcggtgacc tggctgcttt ccagctttgt
    31921 cgcctggaaa caaacaacgg ataacgtcga tgaattgttc gacacccaac tgatgctgtt
    31981 tgccaaacgg ttaagtacgc tcgatctcaa cgaaatcaac gcggcggatc gcatggcaca
    32041 gacgccaaat aaattaaaac acggtcatgt tgatgacgat gcgctgacct ttgccatctt
    32101 tacccacgac ggcagaatgg tccttaatga tggcgataac ggagaagata ttccctatag
    32161 ctatcaacgg gaaggttttg ctgacgggca actggtcggt gaagacgatc cttggcgttt
    32221 tgtctggatg acctcacctg atggcaaata tcgcatcgtt gttggccagg aatgggaata
    32281 ccgtgaagac atggcgctgg cgattgttgc cgggcaattg atcccgtggc tggtcgcact
    32341 gccgattatg ttaatcatca tgatggtact actgggtcgt gaactcgcgc cgctgaacaa
    32401 actggcgctg gcactacgta tgcgtgaccc tgactcggaa aaaccactaa acgcgactgg
    32461 cgtacccagc gaagtgcggc cactggttga gtcgctaaat caactgttcg cccgcacaca
    32521 tgcgatgatg gttcgagaac gacgctttac ctccgatgca gctcacgaac tacgtagccc
    32581 gttaacagcg ctgaaagtgc aaaccgaagt tgcgcagctc tctgacgatg atccgcaggc
    32641 gcggaaaaaa gcactgctcc aattacattc cgggatcgat cgcgctactc gtctggttga
    32701 tcaactgctc acgctatcgc ggctggactc actggataac cttcaggacg tcgcggagat
    32761 cccgcttgaa gatctcctac aatcgtcggt gatggatatt taccacacgg cgcagcaggc
    32821 gaaaattgac gtgcgactga cactcaatgc ccacggcatc aaacgcaccg gacaaccgct
    32881 attgctaagt ttgttggtgc gaaatttgct ggataacggc cccggtgtga caccagaggc
    32941 actggcgcga attggcgaac gcttctatcg cccacccgga caaaccgcta ccggcagtgg
    33001 acttgggcta tcgattgtcc agcgaatcgc caagctgcat gacatgaatg ttgagtttgg
    33061 gaatgcggaa caaggtggat ttgaggcgaa ggtaagctgg taacttctct gtctatgaga
    33121 gccgttaaaa cgactctcat agattttact aatagcaaaa tataaaccgt ccccaaaaaa
    33181 gccaccaacc acaaaccaga caaataacga gcgagaatac atatagccaa aactcgaaca
    33241 tttcccgcat aaagagtttt cttaagataa gaataataag tggcgtaaga agaaaaaatg
    33301 ctgcaatcat tgcttgcaat atacgatctt cgcctgaaac ggaaggtata ctgcaaatat
    33361 tgcctcccga ctcaagcatc cagtcatact tattgaaagc aaaaaaagaa tctattaacc
    33421 aataagtaag cattatatat ggcaatgctt caaatattgt tttaattttc tgctttaaca
    33481 tatatcagta cgctcataaa aatccattta taattatcaa catgacacgt tgatattttc
    33541 atcttttact tattcaaagc catattttca atccccaaaa aaaccgaacc aaacgtgtaa
    33601 attattccgg tgcgagtaag aattatgagg aatggctatc agtattgtca ttttcagaaa
    33661 atatttatcc tgcatcggtg agtcagagct agatcagact tttgctaaat tcgcaaaaga
    33721 ctttgcacat tttgctaatt tcacagtacc gctctgtgac gtactatagt cgacaaacgt
    33781 ctcaccttga gattaaaaaa tgagcaacat cctgattatc aacggcgcga aaaaattcgc
    33841 ccactccaat ggtcaactga acgacaccct gaccgaagtc gcggatggca cactgcgcga
    33901 cctcgggcat gatgtccgca tcgttcgcgc cgacagcgac tacaatgtca aagcggaagt
    33961 acaaaacttt ctctgggctg atgtggtgat ctggcagatg ccaggctggt ggatgggcgc
    34021 gccgtggaca gtgaaaaaat atattgatga tgtattcacc gaaggtcacg ggactctgta
    34081 tgccagcgat ggtcgtaccc gcaaagatcc gtcgaaaaaa tacggttccg gcagcctggt
    34141 acaggacaaa aaatatatgc tttctctaac ctggaacgca ccaatggaag ccttcaccga
    34201 aaaagatcag ttcttccacg gcgttggcgt tgacggtgtg tatctgccgt tccataaagc
    34261 aaaccaattc ctcggtatgg aaccgctgcc gacatttatc gctaatgacg tgataaaaat
    34321 gcctgatgtt ccccgctata ctgaagaata tcgcaagcat cttgtggaaa tttttggtta
    34381 actagagctc aggctttaga aggagttaac catgcttacc gtaatcgcag aaatccgtac
    34441 tcgtcctggt caacatcacc gtcaggcggt attggatcag tttgctaaaa tcgttccaac
    34501 cgtactgaaa gaagaaggtt gccacggcta tgcgccaatg gtggattgcg ctgctggcgt
    34561 gagtttccag tctatggcac cggattctat cgtgatgatt gagcagtggg aaagcatcgc
    34621 gcaccttgaa gcgcatctgc aaaccccgca catgaaggcg tacagcgaag ccgtaaaagg
    34681 cgacgtgctg gagatgaata tccgtattct gcagccaggg atttaatcct gccttgtttg
    34741 cccggccatc ctgaccgggc aatgttcttt cctttaaacc tcaatctccg ccatgtcgcc
    34801 tttctcttgc aaccagttgc ggcgatcttc cgagcgtttc ttcgccagca gcatatccat
    34861 catcgcgtca gtacgctgat cgtcttcatc atcgatagtc aactgcacca gacggcgagt
    34921 gttcggatca agcgtggttt cacgcaattg catcgggttc atttccccca gacctttaaa
    34981 acgctggacg ttcggcttgc ctttcttgcg ttttaattgc tcaagtacgc cctctttctc
    35041 ttcttccgtc agcgcgtaat aaacctcttt cccgagatca atacggtaga gcggtggcag
    35101 tgcgacgtaa acgtgaccgt gtttcaccaa cgcgcggaag tgttttacga acaaagcgca
    35161 gagcagcgtg gcaatgtgca gaccatcaga gtccgcatcc gcgaggatac agattttgcc
    35221 ataacgaagc tggctcagat cgtcgctgtc aggatcgata ccgatcgcta ccgaaatatc
    35281 atgcacttcc tgcgaggcca gcacttcgtc ggaagaaact tcccaggtgt taaggatctt
    35341 acctttcagc ggcatgatcg cctgatattc acgatcgcgc gcctgcttgg cagatccgcc
    35401 tgcggagtca ccttccacga ggaacagttc ggtacggtta aggccctgcg cggtacaatc
    35461 agccagtttg ccaggcagcg ccgggccgct ggtcagcttt ttacgcacca cttttttagc
    35521 cgcacgcata cgacgctggg cgctggaaat cgccatctct gccagcaact ccgccgcctg
    35581 aacgttctgg ttcagccaca ggatgaaggc atctttcacc acgcccgaaa cgaatgccgc
    35641 acactgacgc gaagagagac gctctttggt ctgcccggca aactgcggat cctgcatttt
    35701 aaccgacaga acataggcgc agcgatccca gatatcttcc gccgacagct tcacgccgcg
    35761 cggcagaatg ttgcggtatt cacagaactc acgcatcgcg tccaacaggc cctgacgcag
    35821 accattaaca tgggtaccgc cctgcatcgt tgggataagg ttgacgtagc tttcggtcag
    35881 cagttcaccg ccttccggca gccacagtag cgcccagtcc acagcttcag tatcgccagc
    35941 gaaattaccg ataaacggtt tttctggcag cgtcggtaaa ccgtttaccg cttccgccag
    36001 gtaatcattc agaccgtcct gatagcacca gcgctgttcg gtgttgttga tctcatcttt
    36061 aaaggtaatc tcaacgccgg ggcacagtac tgctttggcc ttcagcacat gcgtcaggcg
    36121 tgaaacagaa aaacgcgggc tgtcaaagaa ggtttcatcc ggccagaagt gcacgctggt
    36181 accggtattg cgtttaccgc aagtgccgac aacctgtaaa tcctgcacct tttcgccatt
    36241 ttcaaaggcg atgttataaa cctgaccatc gcggcgcacg ttaacttcta cgcgcttcga
    36301 caaggcgtta actaccgaaa tccccacgcc atgcaggccg ccagagaact ggtaattttt
    36361 gttagagaat ttaccgcctg catgcagacg gcaaagaatc agttcaaccg ccggtactcc
    36421 ttcttccggg tgaatatcca ccggcatccc gcgcccatcg tcaatcactt ctaacgactg
    36481 gtcagcatgt aagattacat ccacgcgttt tgcgtgaccc gccagtgctt catcgacact
    36541 gttatcaata acttcttgcc ccaaatggtt agggcgagtg gtatcggtat acatccccgg
    36601 acggcggcga accggctcaa gcccggtgag tacctcaatg gcatcagcgt tataagtttg
    36661 cgtcatggtt taagttagca attcgagttg atcgtcagag atggtgcaga ccaagaaaat
    36721 cgacgatcgg gttgaaataa tcttcgaagc ccgtgaatgc gtggttgccg ccttctatga
    36781 cagtctggcg gcaggaagca tagtacgcca ccgcctggcg gtaatccagc acttcatctc
    36841 ccgtctgttg cagcagccag atcaaatccg gcgcttccag cgggtcaatc tgcatgactt
    36901 tgagatcgta aatatggcgt gactctagca catattgctg cccggtgtag gggttctcgt
    36961 tctgaccgag atagtccgtc agcaattcaa acgggcgtac cgccgggttt accaccactg
    37021 cgggcagcat aaaacattgc gacaaccagg tggcgtaata tccccccagt gacgaaccga
    37081 caatacccag cgaatcgccg ccatgttcca ggacaatgga ttccagcagt tctgccgcgt
    37141 cggaaggata cggcggcaac tgcggaatga tcatctcaac gtcagggtga tgttccgcca
    37201 gccagttttt taacaagctc gcttttgcag agcgcggcga gctgttgaaa ccgtgtaaat
    37261 aaagaagcgt agacatcagt agccttctga agcggtatca ggttggaaac gtgtgtccgc
    37321 caggcgatgc acctcggtgg tcagcgtgcc atcagcatgt aactcgagag tacgccagcc
    37381 gggcgcgata gtatccagcg taaagttgga acagtgcggc ttaaactgca cacaggtcga
    37441 cggcgttgcc agcaggcggc gaccgttcca gtcgagatcc agctcctgat gaatatgacc
    37501 gcacagcaag tatttgacgt gcggaaactt cgccagcacg gtatccagtt cgcccgcgtt
    37561 acgcagactg tgttgatcga gccaactaca acccgcaggt agcggatgat gatgcagcag
    37621 cagcaacgta tggcgttctg gcgcatcggc cagtttacgt tccagccact caagctgaaa
    37681 ctcgctcagc tcaccgtgcg gcacgccaaa cacctggcta tccagcaaca ggatttgcca
    37741 ttgctcacca ataaacaccc gcttcgccgg ggagataccc gcatcctgta acgcgctgta
    37801 catcgcgggc tggaaatcgt ggttgcccgg cagccagacg cagggcgcac gaaaacttgc
    37861 gatgccttca gcgaaatgct gataggccgc agaggattga tcctgcgcta aatcacctgt
    37921 cgcgacaatc aggtcgaatt cgtgctggtg tggacgaatc gcctccagca ccgcctggta
    37981 gctttcccag gtgtttaccc ctaacagggc ttcgtgcttt tgtgcaaaca ggtgagtatc
    38041 ggtaatttgt aaaatccttc ctctggcctc accagccaga ggaagggtta acaggctttc
    38101 caaatggtgt ccttaggttt cacgacgcta gtaaaccgga atcgccatcg ctccatgtgc
    38161 taaacagtat cgcaaccagt ccgctaaaaa ctggttaatt tgatgctttt cgtcgcgttg
    38221 atgcaacttt ttattaggat aatcataccg cgctttgaag cgaaaaatct gctggcttga
    38281 acacacttca gccaccatcg cgtcatgata cagacgcacc gtcattgacg gaaggctcca
    38341 gtaactgatc gcgggcgcag tctgttctat tgtcaccagg gtagtgtatc gggtcgattc
    38401 cacaatcgtc agccgatatt gtgcgtttgc cacctgatag cttacagttt cgccgggtgc
    38461 gtcattgcgc ggtaacaaac ggcgcaattg tgaaaagttc atctcgcaca ggcgcatcat
    38521 ttcaggaaag tcaggtgtgt aacgcttcat ttatgcccac tcatttttta acgcttgatg
    38581 atgcagctgc agccattgca aagcgatgac cgacgctgcg ttgtcgattt tcccctcttc
    38641 tacccactgg tatgcctgtt cccggcttac cacatgaacg cgaatatctt cgttttcatc
    38701 agccagacca tgaataccgc ttgcggtcgt ggcgtccact tcgcccacca taattgacga
    38761 acgctcactg gtgccccccg ggcttgccag gaaacttaac accggtttgg tccgtttgac
    38821 tatcagtccc gcctcttcaa tcgcttcgcg acgggcaaca tcttccacac tttcaccctc
    38881 ttcaatcatc ccggcaacca tctccagtag ccagggggtt tcgctggtgt cgtacgcggc
    38941 aatccgaatc tgctcaatca gcacaacttc atcacgcact gggtcaaagg gtagcaagac
    39001 tgcggcgtga ccgcgctcaa aaatttcccg ccgtacctca tgactcattt gcccgttgaa
    39061 tagacgatga cgaaatctat aaagatctaa tgaaaaaaag ccgcgataaa gtgtttctcg
    39121 tgcaataatt tctacatcgt ttttgccaaa tgtaacgggc aggttgtctg gcttaagcat
    39181 tgttaatgtc ctagcactaa tagtgaatta aatgtgaatt tcagcgacgt ttgactgccg
    39241 tttgagcagt catatgttaa attgaggcac attaacgccc tatggcacat taacgcccta
    39301 tggcacgtaa cgccctatgg cacgtaacgc caaccttttg cggtagcggc ttctgctaga
    39361 atccgcaata attttacagt ttgatcgcgc taaatactgc ttcaccacaa ggaatgcaaa
    39421 tgaagaaatt gctccccatt cttatcggcc tgagcctttc tgggttcagt tcgttgagcc
    39481 aggccgagaa cctgatgcaa gtttatcagc aagcacgcct tagtaacccg gaattgcgta
    39541 agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc agtccattac
    39601 tgccacagct aggtttaggt gcagattaca cctatagcaa cggctaccgc gacgcgaacg
    39661 gcatcaactc gaacgcgacc agtgcgtccc tgcagttaac tcaatccatt tttgatatgt
    39721 cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg gattcaggac gtcacatatc
    39781 agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc ttatttcaac gtgttgaatg
    39841 ctattgacgt tctttcctat acacaggcac aaaaagaagc gatctaccgt caattagatc
    39901 aaaccaccca acgttttaac gtgggcctgg tagcgatcac cgacgtgcag aacgcccgcg
    39961 cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg taataacctt gataacgcgg
    40021 tagagcagct gcgccagatc accggtaact actatccgga actggcggcg ctgaatgtcg
    40081 aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct gctgaaagaa gccgaaaaac
    40141 gcaacctgtc gctgttacag gcacgcttga gccaggacct ggagcgcgag caaattcgcc
    40201 aggcgcagga tggtcactta ccgactctgg atttaacggc ttctaccggg atttctgaca
    40261 cctcttatag cggttcgaaa acccgtggtg ccgctggtac ccagtatgac gatagcaata
    40321 tgggccagaa caaagttggc ctgagcttct cgctgccgat ttatcagggc ggaatggtta
    40381 actcgcaggt gaaacaggca cagtacaact ttgttggtgc cagtgagcaa ctggaaagcg
    40441 cacatcgtag cgtcgtgcaa accgtacgtt cctccttcaa caacattaat gcttctatca
    40501 gtagtattaa cgcctacaaa caagccgtag tttccgcgca aagctcatta gacgcgatgg
    40561 aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt gttggatgcg accaccacgc
    40621 tgtacaacgc caagcaagag ctggcgaatg cgcgttataa ctacctgatt aatcagctga
    40681 atattaagtc agccctgggt acgttgaacg agcaggattt gctggcactg aacaatgcgc
    40741 tgagcaaacc ggtttccact aatccggaaa acgttgcccc gcaaacgccg gaacagaatg
    40801 ctattgctga tggttatgcg cctgatagcc cggcacccgt cgttcagcaa acatccgcac
    40861 gcactaccac cagtaacggt cataaccctt tccgtaactg atgacgacga cggggcttcg
    40921 gccccgtctg aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt cttccccctt
    40981 ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg tatttaccac tggtcccgga
    41041 agacaaaaat gaaacggaca aaatccatac gccacgcatc gttccgcaaa aactggagcg
    41101 cacgccatct gacaccagtc gctctcgcgg ttgccactgt ttttatgctg gctggctgtg
    41161 aaaagagtga tgaaacagtg tctctctatc aaaatgctga cgactgttca gctgcaaacc
    41221 caggcaaaag cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa gccgaacgta
    41281 ctgcgccgaa atacgccacc cgtgaagact gtgttgctga atttggtgaa ggtcagtgtc
    41341 agcaagcacc agcccaggct ggcatggcac cagaaaacca ggcgcaggct cagcaatcca
    41401 gcgggagttt ctggatgccg ctgatggccg gttacatgat ggggcgtctg atgggcggcg
    41461 gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa cccggccagt ccggcttacg
    41521 gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc ccagccaggc cgcaccatga
    41581 ccgtaccgaa gacggcaatg gcaccaaaac cggcgaccac cactaccgtt acccgtggcg
    41641 gttttggtga atctgttgcc aaacaaagca ctatgcagcg tagcgcaacc ggcacctctt
    41701 ctcgttcaat gggtggctga taccgatgga aagagtcagt attaccgagc gcccggactg
    41761 gcgtgagaaa gcccacgaat acggtttcaa ttttcacacc atgtacggcg agccgtactg
    41821 gtgtgaagat gcttactaca agttgaccct cgcccaggtt gaaaagctgg aagaagtcac
    41881 cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca gcgatgagct
    41941 gatgaccaaa ttccgcatac caaaacacac ctggagtttt gtgcgtcaat catggctgac
    42001 tcaccagcca tcgctttatt cgcgtcttga tctggcgtgg gatggcactg gcgaacctaa
    42061 acttctggaa aataacgccg atacgccaac gtcactatac gaggcggcgt tctttcagtg
    42121 gatctggctg gaagatcagc ttaacgccgg taacttgccg gagggcagcg accagtttaa
    42181 cagtctgcaa gaaaaactga tcgatcgctt cgttgagctg cgtgaacagt atggcttcca
    42241 gttgctgcat ctcacctgct gtcgcgacac ggtggaagat cgcggaacca ttcagtattt
    42301 gcaggactgc gcaaccgaag ctgaaatcgc caccgagttc ctctacatcg acgagatagg
    42361 tttaggcgaa aaaggccagt tcacggattt acaggatcag gtgatttcta acctgttcaa
    42421 gctttatccg tgggaattta tgctgcgcga aatgttctcc accaagctgg aagatgctgg
    42481 cgtacgctgg ctggaaccgg cgtggaagag cattatctcc aacaaggcac ttctaccgct
    42541 actgtgggag atgttcccga atcacccgaa cctgctgccc gcttattttg cggaagatga
    42601 tcatccgcaa atggaaaaat atgtggttaa accgatcttc tcccgtgaag gcgcaaacgt
    42661 gtcgatcatt gagaacggca aaaccattga agcagcggaa ggtccgtatg gcgaagaagg
    42721 gatgattgtt cagcaattcc acccgttacc gaaattcggc gacagctata tgctgattgg
    42781 tagctggctg gtgaacgatc aacctgccgg aattggcatt cgtgaagacc gtgcgttgat
    42841 cacccaggat atgtctcggt tttatccaca tatttttgtt gaataagcca cgataccgga
    42901 tggcactcgc catccggtaa ttgttagcct atctgcaccg acagcatact taaactgccc
    42961 atttctatac catcgaccgg aatggtaatt ggctcctgcc catcccacac acctaacaca
    43021 tacaacaacg gcagatagtg ctctggcgtt gggttcgata acgcgccacc ttcatggtcg
    43081 agataattca ccagaggatg ctgctctacc ggcccttgcc acgtcagatt cgctttcaca
    43141 tactcattaa acgacgtcgc ccacggatac ggtgaactat caccgtgcca cttcactgtg
    43201 cgcaggttat gcaccacgtt accgctggcg accaacatta ttccttcatc acgcagcgcc
    43261 gcgagtttgc gccccatttc gaaatgccag gcggcaggtt tgctactgtc gatactcaac
    43321 tgcaccatag ggatatcggc gtcaggatac atcttaatca gcacgcccca cgagccgtgg
    43381 tcaaagcccc aggcttcttt atccagcgtc accgggatcg gcgctaacag ctcaaccaga
    43441 cgctgtgcca gcgcaggcga acccggagca ggataatgcg tatcgtacag cgcctgcggg
    43501 aagccaccaa agtcatgaat cgtgggcggc gtctccatcg cggtcactcc tgttccacgg
    43561 gtaaaccagt gagccgaaac caccacaatc gcttgcgggc gtggcaatgt catccccaac
    43621 ttctgccagc tgcgggtata caaattatct tccagcacgt tcatcggact accgtgacct
    43681 aaaaacaatg ctggcatacg tgttgaagac atgatgatat ccttaactaa aggtgtcatt
    43741 ttgatatcct cacaatacgc ttgttcggcg gagtaagaac ccggataaca atgatgatga
    43801 tcatcagtta ttttgacgat ctgcctgaag gtgaagattt ataaggagtt gtcgatgtca
    43861 gtacctctca ttctgaccat actggcgggg gcagccacgt ttattggcgc gtttctcggc
    43921 gttctcggac aaaaaccctc gaaccgctta ctggcgtttt cgctaggttt tgcggcgggg
    43981 atcatgttgc tcatctcatt aatggaaatg cttcctgccg cactagcagc tgaaggaatg
    44041 tcgccggtgt tgggttatgg aatgtttatc ttcggtctgc ttggctactt tggcctggac
    44101 cgcatgttgc cgcatgcgca tccgcaggat ttaatgcaaa aatcggtgca gctgttgcca
    44161 aaatcgatca agcgcacagc cattctgctc actctcggca tcagtctgca taacttcccg
    44221 gaagggattg ccacctttgt cacggcgagc agcaacctgg agctgggatt tggcatcgca
    44281 ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag tggcaggccc ggtttatgcg
    44341 gcaacggggt ctaaacgcac cgcgattctg tgggcgggga tttctggact agcagaaatt
    44401 cttggtggtg tgctggcgtg gttaatcctc ggtagcatga tttccccggt ggtcatggcg
    44461 gcaatcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga attaatgccg
    44521 ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg tggaatgtca
    44581 gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa acaacatcag
    44641 aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt ctaccttttc
    44701 ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca gtgcgtctct
    44761 gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca ccacaaccag
    44821 agcaatcaat acaatccagt accaggcgat aaacatcctt ttacccttaa tatttaacaa
    44881 tactaaacat ttagcgcata aatttcacat atcctttttc ggatatattc atatggtcgc
    44941 ataataacgg aacaattata tgattttgat cataatgaaa aagaatgcct gatattaatg
    45001 aaattttctc atagaaagag aagcggaatt aacatcttgc atagcagggc aaaataaaaa
    45061 ccgaagccag ctacaacttc ggtttcttga ttaaggcagt aattaagcag cggttttcag
    45121 ctggctttac gctcatgtgc ctgacggtat gccaccaggt cttcaatagt cacgagcgcc
    45181 atattgtgtt tattggcaaa ctcaatgcac tctggtgcac gcgccatcgt gccatcgtca
    45241 ttagtcagct cacacagcac accagccggt ttaaagcctg ccagcgtcat caaatcaata
    45301 gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg cctgcgcgcg aagtgggaaa
    45361 acgtggcccg ggcgattcag atcagaaggt tttgcgccat cggcaatcgc tgcgcgaacg
    45421 gtcgtaatac gatcagcggc agaaacaccg gtagtcacac cttcagccgc ttcaatggtc
    45481 acggtaaaac cggtgccata ggcgctggtg ttattttcta ccatcattgg cagatcaagt
    45541 tgtttacggc gatcttcagt gatgcacagg caaacaatac cgctaccgtg gcgaatggtc
    45601 agcgccatct gctcaacggt catggtttct gccgggaaga tcatatcgcc ttcgttttca
    45661 cggtcttcat catcaagcac cattacaccg cgtccttcac gcagcgcagc cagtgcattt
    45721 tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg tctgattcat ggtaaaaaaa
    45781 cctcactaaa attatggtta ccagaatcag ggcagtctta ggagtggcgg catatagcca
    45841 aaataacgtg agcgggtcca tgcccgacag aatcgttact ctctcccatc cggactctaa
    45901 ccgtcggccc cggaattaca ccggatctgc tgtcctttga gttcgcaccc aaagcgctcg
    45961 cgggctttca actgagttga tttaccgccg gtggggaatt tcgccccgcc ctgagaataa
    46021 gcggattcac tataacgcta atgattagcg gcagcaacgc atagcttcac ataattctgg
    46081 tttatgactt acccttatcg cactacaatg gtactcaaca cctatcagta cagggaaact
    46141 acaatgattg acccgaaaaa aattgagcaa atcgctcgcc aggttcacga atcaatgcct
    46201 aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa tccgccaaac cctacaagct
    46261 cagctgacgc gcctcgacct tgtaagccgt gaagagttcg acgtccaaac gcaagtcctg
    46321 ttacggactc gtgagaaact ggcgctgctt gaacaaagaa tcagcgaact ggaaaatcgc
    46381 agtactgaga tcaaaaagca gccagatcca gagactctcc ccccaacgct gtaaacattt
    46441 gtctttaatg cactgtaaat tatgaattaa tcctctaatt tacagtgttt tctatt
//
LOCUS       DAZXUL010000018        46448 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZXUL010000018 DAZXUL010000000
VERSION     DAZXUL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46448
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.018"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            267..392
                     /gene="timP"
                     /locus_tag="ACH3L1_000946"
     CDS             267..392
                     /gene="timP"
                     /locus_tag="ACH3L1_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFT4075792.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(424..1200)
                     /gene="sseB"
                     /locus_tag="ACH3L1_000947"
     CDS             complement(424..1200)
                     /gene="sseB"
                     /locus_tag="ACH3L1_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFT4075793.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1342..2625)
                     /gene="pepB"
                     /locus_tag="ACH3L1_000948"
     CDS             complement(1342..2625)
                     /gene="pepB"
                     /locus_tag="ACH3L1_000948"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFT4075794.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2684..2884)
                     /gene="iscX"
                     /locus_tag="ACH3L1_000949"
     CDS             complement(2684..2884)
                     /gene="iscX"
                     /locus_tag="ACH3L1_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFT4075795.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(2896..3231)
                     /gene="fdx"
                     /locus_tag="ACH3L1_000950"
     CDS             complement(2896..3231)
                     /gene="fdx"
                     /locus_tag="ACH3L1_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFT4075796.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3233..5083)
                     /gene="hscA"
                     /locus_tag="ACH3L1_000951"
     CDS             complement(3233..5083)
                     /gene="hscA"
                     /locus_tag="ACH3L1_000951"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFT4075797.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5100..5615)
                     /gene="hscB"
                     /locus_tag="ACH3L1_000952"
     CDS             complement(5100..5615)
                     /gene="hscB"
                     /locus_tag="ACH3L1_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFT4075798.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5711..6034)
                     /gene="iscA"
                     /locus_tag="ACH3L1_000953"
     CDS             complement(5711..6034)
                     /gene="iscA"
                     /locus_tag="ACH3L1_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFT4075799.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6051..6437)
                     /gene="iscU"
                     /locus_tag="ACH3L1_000954"
     CDS             complement(6051..6437)
                     /gene="iscU"
                     /locus_tag="ACH3L1_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFT4075800.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6465..7679)
                     /gene="iscS"
                     /locus_tag="ACH3L1_000955"
     CDS             complement(6465..7679)
                     /gene="iscS"
                     /locus_tag="ACH3L1_000955"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFT4075801.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7791..8279)
                     /gene="iscR"
                     /locus_tag="ACH3L1_000956"
     CDS             complement(7791..8279)
                     /gene="iscR"
                     /locus_tag="ACH3L1_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFT4075802.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8549..9289)
                     /gene="trmJ"
                     /locus_tag="ACH3L1_000957"
     CDS             complement(8549..9289)
                     /gene="trmJ"
                     /locus_tag="ACH3L1_000957"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFT4075803.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9408..10211
                     /gene="suhB"
                     /locus_tag="ACH3L1_000958"
     CDS             9408..10211
                     /gene="suhB"
                     /locus_tag="ACH3L1_000958"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFT4075804.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            10356..11210
                     /locus_tag="ACH3L1_000959"
     CDS             10356..11210
                     /locus_tag="ACH3L1_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFT4075805.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            11401..12681
                     /gene="csiE"
                     /locus_tag="ACH3L1_000960"
     CDS             11401..12681
                     /gene="csiE"
                     /locus_tag="ACH3L1_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFT4075806.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12673..13812)
                     /locus_tag="ACH3L1_000961"
     CDS             complement(12673..13812)
                     /locus_tag="ACH3L1_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFT4075807.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(13972..14862)
                     /gene="hcaR"
                     /locus_tag="ACH3L1_000962"
     CDS             complement(13972..14862)
                     /gene="hcaR"
                     /locus_tag="ACH3L1_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFT4075808.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            14998..16359
                     /gene="hcaE"
                     /locus_tag="ACH3L1_000963"
     CDS             14998..16359
                     /gene="hcaE"
                     /locus_tag="ACH3L1_000963"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFT4075809.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16356..16874
                     /gene="hcaF"
                     /locus_tag="ACH3L1_000964"
     CDS             16356..16874
                     /gene="hcaF"
                     /locus_tag="ACH3L1_000964"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFT4075810.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            16874..17194
                     /locus_tag="ACH3L1_000965"
     CDS             16874..17194
                     /locus_tag="ACH3L1_000965"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFT4075811.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17191..18003
                     /gene="hcaB"
                     /locus_tag="ACH3L1_000966"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17191..18003
                     /gene="hcaB"
                     /locus_tag="ACH3L1_000966"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFT4075812.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18013..19245
                     /gene="hcaD"
                     /locus_tag="ACH3L1_000967"
     CDS             18013..19245
                     /gene="hcaD"
                     /locus_tag="ACH3L1_000967"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFT4075813.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNA
                     IEHVVDGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTC
                     DPAIFAGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQY
                     SDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSG
                     KTFDAKLLIDENIALKSL"
     gene            19342..19764
                     /locus_tag="ACH3L1_000968"
     CDS             19342..19764
                     /locus_tag="ACH3L1_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFT4075814.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19812..20684)
                     /locus_tag="ACH3L1_000969"
     CDS             complement(19812..20684)
                     /locus_tag="ACH3L1_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFT4075815.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20696..21790)
                     /locus_tag="ACH3L1_000970"
     CDS             complement(20696..21790)
                     /locus_tag="ACH3L1_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFT4075816.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(21823..22821)
                     /locus_tag="ACH3L1_000971"
     CDS             complement(21823..22821)
                     /locus_tag="ACH3L1_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4075817.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22846..24357)
                     /locus_tag="ACH3L1_000972"
     CDS             complement(22846..24357)
                     /locus_tag="ACH3L1_000972"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFT4075818.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24380..25362)
                     /locus_tag="ACH3L1_000973"
                     /pseudo
     CDS             complement(24380..25362)
                     /locus_tag="ACH3L1_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(25459..28740)
                     /locus_tag="ACH3L1_000974"
                     /pseudo
     CDS             complement(25459..28740)
                     /locus_tag="ACH3L1_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            28858..30051
                     /locus_tag="ACH3L1_000975"
     CDS             28858..30051
                     /locus_tag="ACH3L1_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFT4075819.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(30249..31502)
                     /gene="glyA"
                     /locus_tag="ACH3L1_000976"
     CDS             complement(30249..31502)
                     /gene="glyA"
                     /locus_tag="ACH3L1_000976"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFT4075820.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            31829..33019
                     /gene="hmpA"
                     /locus_tag="ACH3L1_000977"
     CDS             31829..33019
                     /gene="hmpA"
                     /locus_tag="ACH3L1_000977"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFT4075821.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33064..33402)
                     /gene="glnB"
                     /locus_tag="ACH3L1_000978"
     CDS             complement(33064..33402)
                     /gene="glnB"
                     /locus_tag="ACH3L1_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFT4075822.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33463..34797)
                     /gene="glrR"
                     /locus_tag="ACH3L1_000979"
     CDS             complement(33463..34797)
                     /gene="glrR"
                     /locus_tag="ACH3L1_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFT4075823.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(34787..35500)
                     /gene="qseG"
                     /locus_tag="ACH3L1_000980"
     CDS             complement(34787..35500)
                     /gene="qseG"
                     /locus_tag="ACH3L1_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFT4075824.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(35665..37092)
                     /gene="qseE"
                     /locus_tag="ACH3L1_000981"
     CDS             complement(35665..37092)
                     /gene="qseE"
                     /locus_tag="ACH3L1_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFT4075825.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(37650..41537)
                     /gene="purL"
                     /locus_tag="ACH3L1_000982"
                     /gene_synonym="purI"
     CDS             complement(37650..41537)
                     /gene="purL"
                     /locus_tag="ACH3L1_000982"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFT4075826.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            41796..43352
                     /gene="mltF"
                     /locus_tag="ACH3L1_000983"
     CDS             41796..43352
                     /gene="mltF"
                     /locus_tag="ACH3L1_000983"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFT4075827.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(43349..43852)
                     /gene="tadA"
                     /locus_tag="ACH3L1_000984"
     CDS             complement(43349..43852)
                     /gene="tadA"
                     /locus_tag="ACH3L1_000984"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFT4075828.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(43910..44545)
                     /gene="yfhb"
                     /locus_tag="ACH3L1_000985"
     CDS             complement(43910..44545)
                     /gene="yfhb"
                     /locus_tag="ACH3L1_000985"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFT4075829.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            44754..45602
                     /locus_tag="ACH3L1_000986"
     CDS             44754..45602
                     /locus_tag="ACH3L1_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFT4075830.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(45941..46448)
                     /locus_tag="ACH3L1_000987"
                     /pseudo
     CDS             complement(45941..46448)
                     /locus_tag="ACH3L1_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11042 a        12779 c        11687 g        10940 t
ORIGIN      
        1 ccagcaggtt gtattaccgt agtaatgcaa gcgcgtctca gcggagacaa tactcgccag
       61 taactctctt tttgtcaagc aaaagagagt tattattgtt ctgttagtgt attatccact
      121 gcggcccttt ccgccgtctc gcaagcgggc gctggcttaa ggaaaggatg ttccgcgacc
      181 gttagagcaa gcgcgtctca gcgcttgcta tcgcggcaat atcgccagtg gtgctgtcgt
      241 gatgcggtct tcgcatggac cgcacaatga agatacggtg cttctgtatc gtacttattg
      301 tttctggtgc gctgttttcc caagtaaata ataaccggag tctctccggc gacaatttac
      361 tggtggttaa caaccttcag agcagcaagt aagcccgaat gccgcccttt agggcggcat
      421 attttagatt atccggtttt gtttaaagtc acgcaaaaaa ccaccccagc gacgttcata
      481 gaatggcgca atatgttcgg taataaagtg gctaattcct ttttcccctt ttttcacctg
      541 acaaatatcg attggttcat cgccaggtaa tgtatcggtc gctacacttc ccgtcgcctg
      601 aataatttct tcgatatcac catcggcttc aatgccaata agtaaattag gctgtgcctc
      661 ttcgttctct ttaattgaac aaataaaagc acgcttcacc ggcttaatgg ttttaaataa
      721 ggtggtgagt gaatcaatca tttgtgctgg cggctctgcg acttccgata atatcagcga
      781 ttcaccgcct tccaggattt cctggctgct cagcggattt ccctcttcac caatcaacaa
      841 actgatttca cgcggcataa attctttacc ggtcggcagt ttggcattaa ggaagagcgt
      901 ttcgccaagg gtcatctcaa acaatgtgcg aacgggcatt acgacaaatg cctgttcgtc
      961 ttcaaccgcc tgttgaagcg cttctaacga ggtgaaaaaa ggaatgacgc tggtgccgtc
     1021 ttctttttcc cagtgctgta aatcaagcgc actatcttca accacagcct cgccctgcgc
     1081 cgccgtacca ggcacccaga cggtggattc cagtagagta cggaaaaagg ccgggcggtg
     1141 cgccggttcg gttgctgctt tttccagcag gtcttcaagt tcgttttttg tttcggacat
     1201 aagaaccaca attcattcaa cgttcggggc gcaaatgcct gatgcgctac gcttatcagg
     1261 cctacaaggg attcgcaatt tgttgaattt gcagaatttg taggccggat aaggcgttta
     1321 cgccgcatcc ggcatattag tttacgccgt taacagatta gctatcgtgc gcacaccaag
     1381 tcccgtagcg cccgcagacc actgttcaac cggcgcttta cggtaagtcg ccgagcagtc
     1441 gatatgcagc cagccttgct gatagttctc aacaaagtgc gacaggaagc ccgccgccgt
     1501 gctcgcgcct gccggatacg ccgcgcttcc ggtattgttc agttcggcaa agttagacgg
     1561 cagctggctg cggtggaatt ccgccagcgg cagacgccag aatggttcgt tctcttgtga
     1621 ggcactcgcc agtaaacgac cggcaagcgc atcgtcaaaa ctgaacagcg cgtgataatc
     1681 attacccagc gcagttttcg ccgccccggt gagggtcgcc gcatcaatga tcatttccgg
     1741 tttctgcgca ctggcatcaa tcagaccatc ggcaagcacc agacgccctt cggcatcagt
     1801 gttcatcact tcaacttttt taccgttgcg ataggtgatg atatcgccca gcttgaacgc
     1861 attgccgcta atcaggttat ccgcacagca gaggaacagc ttcacgcgct tgttcagtcc
     1921 gcgcataatg gcaaatgcca gcgccccggt aaccgttgcc gcgccgccca tgtccgactt
     1981 catcgagtcc ataaacgcag tctgtttgat gctgtagccg ccggagtcaa aagtgatacc
     2041 tttacctacc aggcacgcgt acaccggtgc ttctttatcg ccagttgggt tgtaatccag
     2101 cgccagcaat accggagaac gttctgaacc gcgcccgaca gtgtgcagcc ccatataacc
     2161 ttgctcacgc agatcttcgc ctttggtgat ccgataagtc acacgatcgc ccgcgacgtt
     2221 gctgatcaga tcaacagcac gctgtgccag ttgcgatggt cccaattctt ctgccggtgc
     2281 gttgatggtg tcacgcaccc agtcgatgat catcaggcgg ttatccagtt cctggcgctg
     2341 ggcatcgtcc agatccggcc actccacttt acgcgtgcct ttcgggcctt tataaccttg
     2401 ccagaatgcc cagcagcgat ccgcatccca gccttcaccg cttaactgaa catgcttgat
     2461 gcccagaccg tcaatcttgc gggccgcacg ctggatcagc cccagatcgt ctgccccgtt
     2521 caaatgcagg gtaatgccgt cattattaat gctgtaagtt gctttttctc cccagcgcgc
     2581 gtcggcaggt tgggtagaga gggtaatctt catcgcttct gtcattttag ttatccttat
     2641 tcgtttaaca gcaaaacggg ctacccccag gcagcccgtt cattcactcg gcttcgtcca
     2701 gccagactaa caaaatcgct tcgaggattt tctcgttgga tgcctgcggg tcgtcgtcga
     2761 aatcttccag atcgcaaatc cactgatgca tatcggtgaa tcgaaccgtt ttcggatcaa
     2821 gatcgggata cgcatcgtac agtgcttcgc caatttcgcg gctatcggtc cacttaagtc
     2881 ccatactaac ctctgttaat gctcacgcgc atggttgata gtgtaacgcg ggatttcgac
     2941 cactaaatct tcgtcggtga cgcgcgcctg gcagcttaaa cggctttccg gctccagtcc
     3001 ccaggctttg tccagcatgt cgtcttcctg ctctgagctt tccggcagtg agtcaaaacc
     3061 ttcacgaacg atgcagtggc aggtggtgca agcacaggat ttttcacagg cgtgttcaat
     3121 ctcaataccg ttacgcagcg ctgcgtcgag aatggtttca ccgctattag cttccagaac
     3181 agcgccatca gggcagaggt cctgatgagg caaaataaca atctttggca tattaaacct
     3241 cgtccacgga atggcctttc agcgcacgac gaaccgactg gtccatgcgg cgagcggcga
     3301 aatcctgggt ttgtttgtct acgtttttaa tcgcttgttc gatggcgtca acatcatcgc
     3361 cctgcgccac ttcactcagg tgagcggcag cattgtcaat gacctgacgc tctgcggcgc
     3421 ttaacagcgc ggcatcagca gccagcgcgc cgtgcagact ttccagcaca cgcgccgctt
     3481 ctactttttg ttctgccagc atacgggctt ttacgtcctg ctcggcatag ctcattgagt
     3541 ctttgatcat cgaagcgatt tcgctatcgg tcagaccgta agacggtttg acctgaatag
     3601 acgcctcaac gccggtggat ttctccatcg ccgtcacgct caaaagaccg tcggcatcga
     3661 cctggaacgt cacgcgaata tgcgcaccgc cagccggtag cgccggaata ccacgcagcg
     3721 caaaacgcgc cagtgagcgg cagtcctgca ccagttcgcg ctcaccctgc attacatgga
     3781 tagacatcgc cgtctgacca tctttaaagg tggtgaaatc ctgagcgcgg gccaccggaa
     3841 tagtggtatt acgcggaatc actttctcca ccaggccgcc catcgtttcg aggcccagcg
     3901 acagtgggat cacatcaagc agcagcattt cgctgtctgg cttgttaccc accagaatat
     3961 ccgcctgaat cgccgcgcca atagcgacga ctttatccgg gtcgatggaa gtcagcggtg
     4021 gacgaccgaa aaattcgcct acccgttcac gcaccagcgg cacgcgagta gaaccgccca
     4081 ccatcaccac ttccagcact tcgtcagctt ctacacccgc gtctttcagc gcgcgacgac
     4141 aagccagtaa ggttcgtttt accagtggcg cgatcagttc attgaattgt tcacggctga
     4201 tttcgccctg ccagcccgca acgttaacgg tcacggagtc cgcatcgctc agcgcgattt
     4261 tggctgcaat ggtggcatcc agcagttcac gctgaacgcg gttatcgcta cgatcaggaa
     4321 tgtccgcctg ctcgcgaatg taatccgcca gcagatggtc gaaatcatcg ccgccgagcg
     4381 cggaatcacc gccggttgcc agcacttcaa acacgccgcg acttaagcgc agaatggaaa
     4441 tatcaaacgt cccgccaccg aggtcataaa cagcgatcac gccttcctga ccggaatcca
     4501 gcccgtaggc gatagccgca gcagtcggtt cgttaagtaa gcgcagaaca tgcaaccccg
     4561 ccagacgcgc cgcgtctttg gtgccctgac gctgggcatc gtcaaagtac gccggaacgg
     4621 tgataactac accatccagc tcgcctgcca gggcttcagt tgcccgcgcc gccagtgctt
     4681 tgaggatgtc cgcagaaacg cgcaccgggt tcagtagccc cgccgccgtt tcaatcatcg
     4741 gcaggccgtt ttcgctggcc tggaattgat aaggcagatg cggatagcgt tgctggatat
     4801 cagccagcga gcgccccatc aggcgtttaa cagaactaat ggtgttggcg gtgtcaagcg
     4861 ctgcgttagt acgcgcgtca taacccaccg aatgcccttg ctgttgatag tgaacaacag
     4921 atggcagcag gtgacggcct tcatggtcgg ctaacgtttc ggcctgaccg ctgcgcactg
     4981 tcgccaccag cgagttggtt gtgcccaggt caataccagc cgccagacga cgctgatgcg
     5041 gcgcagcact caaaccaggt tcactaattt gtaataaggc catgtttagc ttccagaaat
     5101 taaaaatcga gcagtttttc ttcgagttgt tcggcactgc tccgcagttt atcgagaaaa
     5161 cgcagcttac gcaccgtatc cgccgccgcg tcccacgcct cgttgtctaa ctgttcaacc
     5221 atcaactgat ggcgggtatc aaacatcttt ttcacacgtt tgataaagct ttccagccgc
     5281 gcttcatctt tcgcctgttc gatctcgtcc agctcttcgc gcagctccaa ctgttccatc
     5341 aggaacgcgg tgtcgcgcac agtatgctgc tcgctggcga gatcaaaacc gtgtaaagaa
     5401 agcaaatatt ccgcgcgcat taacggatga cgcagcgttt gccaggcctg gttaatggtt
     5461 gcagattgct gtacggcggc gagttgttcc gccaggcttc cgctggcgaa tttatcagga
     5521 tgatactgac gttgtagatc ctgaaaacgc aggctcagtg cctgggtatc gagttgataa
     5581 cgggcaggca agccaaagag ggtgaagtaa tccataacat tctcagggct gcgatccgcc
     5641 gcgataacgg cgaatcaggt aaaacaaacc ccacgcgcag gcgaccacgg tggggttatc
     5701 ggtatgcgca tcaaacgtgg aagctttcgc cgcaaccaca ctcatcttta acgttcgggt
     5761 tggtgaattt aaacccttca ttcaggcctt cttttacgaa gtccagctgc gtaccgtcca
     5821 gaaattgcag gcttttgcca tcgaccacga ctttcacgcc tttgtcttca aacacgatgt
     5881 cttccggcgt cggttcgtca acaaattcca gtacataagc catacctgaa catccggagg
     5941 ttctcacgcc cagacgcagg ccaaaccctt tgccgcggtt agccagaaag gtatttactc
     6001 gcgctgctgc actgtcgctc agtgtaatcg acataaccaa acctcaactc ttattttgct
     6061 tcacgtttgc ttttatagtc cgcaatggcg gctttgatcg cgtcttctgc cagaatagaa
     6121 cagtgaattt tcaccggcgg cagttcaagt tcttcagcaa tatcggtgtt tttgatcgcc
     6181 tgcgcttcgt cgagagactt ccctttcacc cattcggtga ccagggagct ggaagcgata
     6241 gcggaaccgc agccgtaagt tttaaaacgc gcgtcttcaa tgataccttc atcgttgact
     6301 ttaatctgca acttcattac gtcgccacag gccggtgcac ccaccatgcc gctgccgacg
     6361 ttctcgtcgt tgttatcaaa ggaacccacg ttacgcggat tctcgtaatg gtcgataact
     6421 ttttcgctgt aagccattat aaattctcct gattccgata ccgattaatg atgagcccat
     6481 tcgatgctgt tcagatccac gccctgcttg tacatttccc acagcggaga aaggtcacgc
     6541 agacgaccga tggatttacg aactaactcg atggtgtagt cgatctcttc ttcagtagta
     6601 aaacgaccta aagagaaacg gatagagcta tgtgccagct cgtcgttcag ccccagcgcg
     6661 cgcagcacgt aggacggttc gaggcttgct gacgtacagg cggaacctga agaaactgcg
     6721 aggtctttca gcgccataat cagcgactca ccttcaacgt agttgaagct gacgttgaga
     6781 atgttcggcg caccgtgttc caggtcaccg ttcaggtaaa cttcttcgat atctttgatg
     6841 ccgttccaca gacggttacg caggccgcgc agacgttcca tctcggtcgc catctcttct
     6901 tttgcgatgc gataggcttc gcccattccg acgatctggt gaacaggcag agtgccggaa
     6961 cgcataccgc gctcgtgacc gccgccgtgc atttgcgctt cgatgcgtac acgcggttta
     7021 cgacgcacat acagcgcacc gatacctttc gggccataga ttttgtgacc ggagaaagac
     7081 atcaggtcaa ctttcaactg gctcaggtcg attggcagtt tgcccacgct ctgggttgca
     7141 tcaacgtgat agataatgcc acgagcacgg cacatttcgc cgatagccgc gatatcctgc
     7201 accacgccga tttcgttatt tacgtgcatg atggaaacaa ggatggtgtc gtcacgcatc
     7261 gccgcttcga gttctttcag gtcgataata ccgttacgct gcggtgccag gtaggtgact
     7321 tcgaaacctt cgcgctccag ctgacggcaa gtatccagta ccgctttgtg ttcggttttg
     7381 ctggtgatga tgtgcttgcc ttttttctga taaaagttgg ctgcaccttt gatcgccagg
     7441 ttgtcagatt cggttgcacc agaggtaaag acgatttcac gcggatcagc gccgaccaga
     7501 tcggcaatct gattacgggc gatatctacc gcttcttcag cctgccagcc gaaacggtga
     7561 gaacgagagg ccgggttacc aaaggttccg tccatcgtca tgaactgcat cattttctcg
     7621 gcaacacgcg ggtccaccgg cgtggttgcg gagtagtcga gataaatcgg taatttcatt
     7681 gctctataaa ctccgtacat cactcaatgc aaggaatcag gctaccggct ggatgtacga
     7741 ccgtgtttac ggagtattta gcactccggc ctgattctga attcttttta ttaagcgcgt
     7801 aacttaacgt cgatcgcgtc ttgtgtgcgg gtgcgtggcg cgtcgtgagt atgctgacga
     7861 ccagacacat ccagcacttc ctggttatta accagttcgc ctaaagtaat gttgttgaga
     7921 aaaccggtga gacggtcgct caaatcacgc cacagcgcgt gggtcaggca tttatcgccg
     7981 ccctggcagc cgcctttacc ctgacaacgg gtggcatcta cagattcgtc aacggcgcta
     8041 atcacttcgc caacggcgat gctgctggca tctttgccta acagataacc accgcctggt
     8101 ccacgtacgc tggaaaccag accattttta cgcagacggg aaaacagttg ttccagataa
     8161 gaaagggaaa ttccctgacg ttcggaaata tcagccaacg gtaccgggcc cgcttcagag
     8221 ttgagcgcaa cgtcaagcat tgcggtcacg gcatagcgcc ctttagatgt cagtctcatg
     8281 tcttacttca cctcaaactc gcccctgccc ggggtttttt attgtaaaag tgggggtatt
     8341 gcatagcagg gtcaagtctg acattcccga gtaaattggt caactattta cttgactgat
     8401 ttagtcgggt atttaacctt cagtgccatt tttttatcgt ggcgtttgta cctgttgtcg
     8461 gatgcggtgt gaacacctta tccgacctac gattctgtac attgtaggcc tgataagacg
     8521 cgttaagcgt cgcatcaggc aaccactgtt attcggcctt gttaccttta ttctgctgct
     8581 caatagaagc cagaatcccg cgcaggatat tcaattcctg gctttccggg cgcgcacggg
     8641 taaacagacg gcgcaattta ttcatcacct gccccggatg gttttcacgg ataaaaccgg
     8701 ttgccagcag cgtttgttcc agatgaccat aaaaacgctc cagatcatcg accagcggat
     8761 acggcgtctc ttcatgctcg acctgctcgc cattttcctg agttgccagc caggccatac
     8821 gcacttcata ggcaataacc tgaaccgcca ttgccaggtt cagcgaactg tattccgggt
     8881 ttgccgcaat cgcgacatga taatggcatt tctgcaactc ttcattggtc aagccgacgc
     8941 gctcacgacc aaacaccagc gccaccgggg tatttgccgc ttcagcgacg cttttcagac
     9001 cgcattcgcg cgggtcgagc atcggccacg gcagcgtgcg ggagcgtgcg ctggtgccca
     9061 ccaccaggct acaaccggct aacgcttcgt cgagcgtatc gacgatgtga gcattaccaa
     9121 tcacatcgct ggcccctgca gccagggcaa tcgcctggga gtcgggtttc accagtggat
     9181 taaccagcca cagattggtt aatcccattg ttttcatggc acgggcaaca gaacccatat
     9241 tgccggtgtg tgacgtctcc accagcacaa ttcgaatatt ttgcagcatt gtctttcttc
     9301 atctaaagat tattcacgca tcttatcata aaacgaagac agatgccgat ctcgctgcta
     9361 tactctgcgc cgttttcccg ttctttaaca tccagtgaga gagaccgatg catccgatgc
     9421 tgaacatcgc cgtgcgcgca gcgcgcaagg cgggtaattt aattgccaaa aactatgaaa
     9481 ccccggacgc tgtagaagcg agccagaaag gcagtaacga tttcgtgacc aacgtagata
     9541 aagctgccga agcggtgatt atcgacacga ttcgtaaatc ttacccacag cacaccatca
     9601 tcaccgaaga aagcggtgaa cttgaaggta ctgatcagga tgttcaatgg gttatcgatc
     9661 cactggatgg tactaccaac tttatcaaac gtctgccgca cttcgcggta tctatcgccg
     9721 ttcgtatcaa aggccgcacc gaagttgctg tggtatacga tcctatgcgt aacgaactgt
     9781 tcaccgccac tcgcggtcaa ggcgcacagc tgaacggcta ccgtctgcgc ggtagcaccg
     9841 ctcgcgatct cgacggtacc attctggcga ccggcttccc gttcaaagca aaacagtacg
     9901 ccactaccta catcaacatc gtcggcaagc tgttcaacga atgtgcagac ttccgtcgta
     9961 ccggttctgc ggcgctggat ctggcttacg tcgctgcggg tcgtgttgac ggtttctttg
    10021 aaatcggtct gcgtccgtgg gatttcgcgg caggcgagct gctggttcgt gaagcgggcg
    10081 gcatcgtcag cgacttcacc ggtggtcata actatatgct gaccggtaac atcgttgctg
    10141 gtaacccgcg tgttgtgaaa gccatactgg cgaacatgcg tgacgagtta agcgacgctc
    10201 tgaagcatta atgactcagg cgggtgatat cactcacccg ccctcgcctt tcaggcgcta
    10261 ttccgaaata cttcctcacc gctttacttt ctttcgtcac tctcccacca tctttccccg
    10321 attaatggat gataaagaag tcgcaaggaa aggttatggc actgccagtg aacaaacgcg
    10381 ttcccaaaat tctgtttatt ctctttgttg ttgccttctg cgtttattta gtgccgcgcg
    10441 ttgccatcaa cttcttctat tatcctgacg ataaaattta cggtcccgac ccctggtcgg
    10501 cggaatccgt cgaatttacg gctaaggacg gtactcgtct gcaaggctgg tttattcctt
    10561 cttcgacggg ccctgctgac aacgccatcg caaccatcat tcatgctcac ggcaatgccg
    10621 gaaatatgtc cgcccactgg ccgctggtca gttggttacc tgagcgtaat ttcaacgttt
    10681 ttatgtttga ttatcgcggg tttggtaaat caaaaggcac tccgtcccag gccggattgc
    10741 tggacgatac gcaaagcgcc atcaatgtgg tgcgccatcg cagtgatgta aacccacaac
    10801 agctggtgct gttcgggcag agtattggcg gggcgaatat tcttgctgtt attggtcagg
    10861 gtgatcgtga aggcatacgt gcggtgatcc tcgactccac atttgcctct tatgcaacca
    10921 tcgccaacca gatgatcccc ggcagtggct acttacttga tgagagttac agcggcgaaa
    10981 attacatcgc cagcgtcagc ccgatcccgc ttttactcat tcacggtaaa gccgatcacg
    11041 ttatcccatg gcagcacagc gaaaagttgt atagcctggc aaaagagcca aaacggttga
    11101 tcctaatccc ggatggcgaa cacattgatg ctttttccga tcgtcacggc aatgtttatc
    11161 gcgaacagat ggtggacttt atccttagcg cgttgaatcc gcagaactaa cccatgatcg
    11221 ctagcacgat aatcattcac aaaaccacct taagacatgc taatccactg gtcagaacag
    11281 tttaaaatga gaaaaattct gtgacgcttg ccaacatttc tgatgattag cattcccttc
    11341 gccatttccc tgagcaaact ttagctattc ttatcaatta tgcttatggg agatctacag
    11401 atgatgccta cgcttgctcc accatctgtc ctttcggctc cccagcgccg ctgccagatc
    11461 ttgctgacgc tctttcagcc ggggttgacc gccaccatgg caaccttcag cgagcttaat
    11521 ggtgtggatg atgatattgc cagtcttgat atcagcgaaa caggacggga gatcctgcgc
    11581 tatcatcaac tcacactgac gactggttat gacggtagct accgggttga aggtacagtg
    11641 cttaaccaac gtttgtgttt atttcactgg ctacgacgtg gtttccgtct gtgtccgtca
    11701 tttattacca gccaattcac ccccgccctg aagagtgaac tgaagcggcg cggaattgcg
    11761 cgtaactttt acgacgatac caatctacaa gcgttagtga atctctgctc ccgacggctg
    11821 caaaaacgct ttgaatcgcg cgatattcat ttcctgtgtc tgtatctgca atattgtttg
    11881 ctgcagcacc acgctggaat tacgcctcag tttaatccgc tccaacgtcg ctgggcggaa
    11941 tcctgccttg aatttcaggt agcgcaggaa attggacgcc actggcagcg tcgggcgctc
    12001 cagcatgtac cgcctgatga gccactgttt atggcattac ttttttccat gttgcgggtt
    12061 cccgatccac tgcgggatgc gcatcagcgg gacagacaat tgcgtcagtc tatcaaacgt
    12121 ctggtaaacc attttcgtga gctgggaaat gttcgttttt atgatgaaca ggggttatgt
    12181 gatcagcttt atatccacct cgcccaggcg ttaaatcgca gtttgtttgc catcggtatt
    12241 gataataccc tgccggaaga gttcgccaga ctgtatccac gcctggtacg caccacccgc
    12301 gcggcgctgg ccggatttga aagtgaatac ggcgtccatc tttctgatga ggaaagtggt
    12361 ctggtcgcgg tgattttcgg tgcctggcta atgcaggaaa acgatctgca tgaaaaacag
    12421 attattctac tgaccgggaa tgatagcgag cgagaagcgc agattgagca gcagttacgc
    12481 gaactaacgt tactgccgct caacattaag catatgtcgg taaaggcatt tttgcagaca
    12541 ggcgctccgc gtggcgcggc actgattatt gcgccttata ccatgccgtt accgctcttt
    12601 tcaccaccgc tgatctatac ggacctgacg ctgacaacac atcaacagga gcagatccgc
    12661 aaaatgctcg aatcagcatg agggaacaac tttcgggcgc aaaaacattg ccggaagcgc
    12721 caccagcgcc attacccaga acacgccgtg gcccagatat tgatacagga aaccggcgaa
    12781 aacggtcatg atagcgatac tgccgcccat cgcgacggca gagtaaaccg cctgtaaacg
    12841 gatgacttcg ctaacctggc gagcagcaat ataacgcatg gcggccaggt ggcagaccgt
    12901 gaaggtgccg caatgcagaa tttgcaccac tatcaaccac ggcaacgccg tagttgctcc
    12961 cataatgccc cagcgcacta cgccgcaaat cgccgagatc aacagcatat cgcgagcact
    13021 acaacggcgg aaaagtttat tactcagcgc aaagataatg acttccgcca ccacgcccag
    13081 cgaccacaaa taccccaccg ccgaggccga gtagccagct gcctgccagt aaatggcgct
    13141 aaaaccgtaa taggccgcat gtgccccctg caataaacaa acgcaggcca gaaagcgcca
    13201 gttctggcga accagcgcca accacgcaga ccaaccggtg ctctcctgct ggcggcttgc
    13261 cccttgtggc tgaatcgtcg gacggatgag aaagccgagc agcatggatg ccacgcccaa
    13321 cgtcaacagc acgaggatca cccgataatc aaacatagtg accagtttgc ccgtcagcgc
    13381 cgagccaatg acaaacgcca ccgagcccca cagtcgcact ttgccgtaat caagcgggaa
    13441 ctgcttttgc cacgtattcg ccagtgcatc ggtcaacggt accagcggtg agaaaaagag
    13501 gttaaagcca atcatcacca gcatcagcca cgctacgtgc gcccccgccc agaaggcgac
    13561 agcaaagaga agtggcagca gtgccagcac gcgcaaggcg gaaatcaggc gggaaggatc
    13621 gctgacgcgg ggcgcgatga gcaaactccc gagaaaacgg gcaaccagac ctgcccccaa
    13681 taacaggccg atggtttctg gcgttaaacc aatcccttta agccagacgc tccagaaagg
    13741 taaaaaaatg ccgtaactaa aaaagtatgt gaaatagccg agcgccaacc agcgcgtgga
    13801 ttgcaaaacc atgagtccct cccgtcagga ggcgttagtc tggggttaat cgaatgttgg
    13861 tgcaagttga aaaataagaa tattcgagcc tgcttcccga tgtctgtaac gccagttcac
    13921 aagcactaaa aaactgcgat ccgcgacgca tgttgcaaaa ataatgcgta attatgccgt
    13981 tacgcttgcc aaacgttcct gcacaatggc gatgaaatcg cgtaatgcca gcttcatctc
    14041 ccctttcttc cacgccatca gtaaagcaat ggaaggcaca tttccggcaa tgggacgaaa
    14101 aacaacctgc ccggtattaa aattattcat ataaccgggt atcaaagtga cgcccagccc
    14161 catgcccacc agattcatgg tcaccagaat attcgttgcc acctggacga tatttggctg
    14221 gctgttttct tgcgcaaacc acgctttaac gatcggcgca agcgaaccgg aatacgccgg
    14281 atcggtactg acgaaattca cgccatccag ttgcgcggcg gtgatctctt tttcatgtgc
    14341 taaagggtga tcaaccggta acaccaccac taatggttcg tcaaaaagct ccagataatc
    14401 aatctccggg ctataaacag gatggcgcat taagccgacg tcgagttcac cacggcgaat
    14461 tttttcctcc tgttgcgtgg tgattaaact caccagctca atcaaggtgt ctggctgtct
    14521 gagacgaaac atcggtaata cttttggcaa taaattcact tccgccgatg gcacaaagcc
    14581 aatggttaat tgtctgtctt cctgaacaat tttccgcgcc cgtaatttgg cattttccgc
    14641 ttgttcgagg attgccagcg cgtcctggag aaaacattct cccgccgccg tcaacgcgac
    14701 tttgcgctta tccctcacca gtaacggaac accgacacag ttttcaagat cgcggatctg
    14761 gctgcttaac gaaggctgtg aggtatgcag tttttccgcc gcacgggtaa agttcagtgc
    14821 ctgcgccact gcgacgaaat agcgtaaatg ccgtagttcc atcaccttcc ccttgttatc
    14881 gaaaaaacgt ctcagctggt agaaattaac tatttcacat attagcaacc aaccagcaac
    14941 atccttatgg cacaaaaata gaaggtcaat acatcttatc tttcaggatt aaaaaatatg
    15001 accacaccct cagatttgaa catttaccaa ctgattgaca cccaaaacgg tcgggtcact
    15061 ccgcgtattt ataccgaccc ggacatttac caactggagc ttgagcgtat tttcggtcgt
    15121 tgctggctat ttctcgccca cgaaagccag atcccaaaac ccggtgattt ctttaacacc
    15181 tacatgggag aagatgcagt tgtcgtggtg cgccagaaag acggcagcat caaggcgttt
    15241 ctcaaccaat gccgccaccg ggccatgcgt gtgagttatg cagattgtgg caacagtcgc
    15301 gcctttacct gcccgtatca cggctggtct tatggcatta acggcgagtt gatcgatgta
    15361 ccgctggaac ctcgcgccta cccacaaggg ttgtgtaaat cccactgggg gctaaacgaa
    15421 gttccttgtg tggagagtta taaaggactg atttttggca actgggatac cagcgcaccg
    15481 ggcctgcatg attacctggg tgacattgcc tggtatctgg atggcatgct ggatcgtcgc
    15541 gaaggcggca ccgaaattgt cggcggcgta caaaagtggg tgatcaactg taactggaaa
    15601 ttcccggcag agcagttcgc cagtgaccag tatcatgctc tgttcagcca tgcttctgcc
    15661 gttcaggtat taggggcgaa agatgatggc agcgataagc gcctcggtga tggacaaacc
    15721 gcccgcccgg tgtgggaaac cgccaaagat gcgctgcaat ttggtcagga cggtcacggt
    15781 agcggtttct tctttactga aaaaccggat gctaatgtct gggtcgatgg cgcagtttca
    15841 agctattacc gcgaaaccta tgccgaagca gaacaacgtt taggtgaagt tcgcgccctg
    15901 cgcctggcgg gtcataacaa tatattcccc acgctttcat ggctaaacgg cactgccaca
    15961 ctccgcgtct ggcatccgcg cggccctgat caagttgaag tgtgggcgtt ctgtattact
    16021 gacaaagccg cctccgatga agttaaagcc gcttttgaaa acagcgccac tcgtgctttt
    16081 ggtcctgctg gttttctcga gcaggatgac tcggagaact ggtgtgaaat ccagaaattg
    16141 cttaaaggcc accgcgcccg caacagcaaa ctgtgtctgg aaatggggct tggtcaggaa
    16201 aagcgccgcg acgacggcat tcctggcatt actaactata tcttttcaga aactgccgct
    16261 cgtggaatgt accaacgctg ggccgatctt ctgagtagcg aaagctggca ggaagtgctc
    16321 gataaaaccg ccgcttacca gcaggaggtg atgaaatgag tgcgcaagtt tcactggagt
    16381 tacatcaccg cattagccag tttctctttc acgaagccag tctgctggat gactggaaat
    16441 ttcgtgactg gttggcacag ctcgacgaag agattcgtta caccatgcgc accacggtta
    16501 acgcgcaaac acgcgaccgt cgcaaaggcg tccagccgcc gacgacctgg attttcaatg
    16561 acaccaaaga ccagctggag cggcgaatcg cccgtctgga aacgggcatg gcctgggcag
    16621 aagagccgcc gtcacgcact cgtcacttaa tcagcaactg ccaggtaagc gaaaccgaca
    16681 tcccaaacgt atttgccgtg agggtgaatt atctacttta tcgggcacaa aaagagcgcg
    16741 atgaaacatt ctatgttgga acgcgtttcg acaaagttcg ccgtctggaa gatgacaact
    16801 ggcgcttgct ggaacgggat atcgtcctgg atcaagcggt gatcacttcc cataacctga
    16861 gtgtactgtt ctgatgaatc gaatttatgc gtgtcccgtt gcggatgtgc cggagggtga
    16921 ggctctccgg atagatacct cgcccgtcat cgccttgttc aacgtgggcg gcgagtttta
    16981 tgccattaac gatcgttgca gccatggtaa tgcgtcgatg tcagaagggt atctggaaga
    17041 tgacgccacg gtggagtgcc cgctacacgc cgccagtttt tgcctgaaaa cggggaaagc
    17101 gttatgcctg cccgccaccg atccgctcac cacttatcca gtacacgttg aaggtggtga
    17161 cattttcatc gacttaccgg aggcgcagcc atgagcgatc tgcataacga gtccattttt
    17221 attaccggcg gcggatcggg attagggctg gcgctggtcg agcgatttat cgaagaaggc
    17281 gcgcaagttg ccacgctgga actgtcggcg gcaaaagtcg ccagtctgcg tcagcgattt
    17341 ggcgaacata ttctggcggt ggaaggtaac gtgacctgtt atgccgatta tcaacgcgcg
    17401 gtcgatcaga tcctgactcg ttccggcaag ctggattgtt ttatcggcaa tgcaggcatc
    17461 tgggatcaca atgcctcact ggttaatact cccgcagaga cgctcgaaac cggcttccac
    17521 gagctgttta acgtcaacgt actcggttac ctgctgggcg caaaagcctg cgctccggcg
    17581 ttaatcgcca gtgaaggcag catgattttc acactgtcaa atgccgcctg gtatcctggc
    17641 ggcggtggcc cgctgtacac caccagtaaa catgccgcaa ccggacttat tcgccaactg
    17701 gcttatgaac tggcaccgaa agtgcgggtg aatggcgtcg gcccgtgtgg tatggccagc
    17761 gacctgcgcg gcccacaggc gctcgggcaa agtgaaacct cgataatgca gtctctgacg
    17821 ccggagaaaa ttgccgccat tttaccgctg caatttttcc cgcaaccggc ggattttacg
    17881 gggccgtatg tgatgttggc atcgcggcgc aataatcgcg cattaagcgg tgtgatgatc
    17941 aacgctgatg cgggtttagc gattcgcggc attcgccacg tagcggctgg gctggatctt
    18001 taaggaagca cgatgaaaga aaaaacgatc attattgtcg gtggcgggca agcggcggca
    18061 atggctgcgg cctcgctacg ccagcaaggg ttcaccggtg agctgcatct gttttccgat
    18121 gagcgacatc ttccttatga acgacctcca ctctcgaaat ccatgttgct ggaagattcc
    18181 ccgcagttac agcaggtgtt acccgctaac tggtggcagg aaaacaatgt tcatctgcat
    18241 tccggtgtaa ccatcaaaac gctgggtcgc gacacacgag agttagtgtt aaccaacggc
    18301 gaaagctggc actgggatca gctttttata gcaaccggcg cggcagctcg accgctgccg
    18361 ttgcttgatg cactgggaga acgctgcttt accctacacc atgccggtga tgccgccaga
    18421 ctgcgagaag ttctgcagcc cgaacggtca gtcgtgattg tcggtgccgg aactattggt
    18481 ctggaactgg ctgccagcgc cacgcagcgc agcgcagcgc agcgcagcgc agcgcagcgc
    18541 agatgtaagg tgacagtgat tgaactggcg gcaaccgtca tgggccgtaa tgcaccaccg
    18601 cccgtgcaac gctatctttt acagcgccat cagcaggctg gtgtgcgcat tctgctcaat
    18661 aatgccattg aacatgtggt cgatggtgaa aaagtagaac tgacgctgca aagtggggaa
    18721 acgcttcagg ctgatgtggt gatttacggt attggtatca gcgccaacga gcaactggct
    18781 cgcgaggcca accttgatac tgccaatggc attgtcattg atgaggcttg ccgcacctgc
    18841 gatcccgcga tctttgccgg tggcgatgtg gcaatcactc gtcttgataa tggtgcacta
    18901 caccgctgcg aaagctggga aaacgccaat aaccaggcgc aaattgccgc tgccgcaatg
    18961 ttagggctac cgctaccgct actgccgccg ccgtggttct ggagcgatca gtacagtgat
    19021 aacttacagt ttattggcga tatgcgtggc gatgactggc tttgtcgtgg caacccggaa
    19081 actcagaagg cgatttggtt taatctgcaa aacggcgtgc ttatcggtgc ggtcacgctg
    19141 aatcagggcc gtgagattcg cccaattcgc aaatggatcc agagcggcaa aacgtttgat
    19201 gcgaaactgc tgatagatga gaacatcgcg cttaaatcac tgtaatcagg ataattagcg
    19261 aatatctcaa tgcctggggc gtggcgaggt gcaagagtgt gtattacgtt taaatcacat
    19321 tatcttgcaa agggattggt tatgaacaca ctacgttatt ttgattttgg agctgcccgc
    19381 cccgttttgt tattaattgc ccgtatcgcc gtggtcttaa ttttcattat ttttggtttt
    19441 cccaaaatga tgggctttga cggtacggtc caatatatgg cctcgttggg cgcgccaatg
    19501 ccgatgctgg cagcgattat tgcggtagtt atggaagtgc ccgccgcgat attaatcgtg
    19561 cttggctttt tcacccgtcc gctggcggtg ctgtttattt tctacacgct gggtacggcg
    19621 gtgattggtc accattactg ggatatgacc ggcgatgcgg ttgggccaaa tatgattaat
    19681 ttctggaaga atgtcagtat cgctggcgcg ttcttgctat tggcaattac cggaccgggg
    19741 gcaatttctc tcgatcggcg ttaggaaaga tgccggatgc ggcgtgaacg ccttatccgg
    19801 cattttataa attacagcca ctctacccgc aacgacatct ctgaggttgt tgattcccct
    19861 ggtgctagcg caatgagatc acccccttcc gggcgatgat gctcatccgg cgcatggctc
    19921 atcggttcca gacagaagaa atcaaacgca tatcctttat caaacgcagg gtcggaaaca
    19981 aagatgaaat aacacggtgc aggtggcgtc gtttccatga tgatggcata accttcctgc
    20041 ggctgctcga tgcgggcctg accattccat ccggcaaaac cattgttcac ccactgacgc
    20101 ggcaacggcg cgagctggtt aaaatccagt tcctgcggta gctgctcgca aaactcaccc
    20161 gccagccact gctcccgttc cagccagtaa ccgctcgcct gcgcctgaat ccgcgtttgt
    20221 ggtgacaacg gaaaataagg atgccagccg gtaccaaatg gcagcgtctc tgccccttga
    20281 ttggtgacag agagcgtcac cgtcagcgta tccgccgtta aatgaaacgc ctgacttacc
    20341 cgatagtgat agacaccgct gcgatgttca tacaccagac acaaactatc atcgctgtgc
    20401 gagacacatt gccactcgcc cagccagcca tcgccgtgta gatagtgcgc atcccactca
    20461 acattcggtt gcagttgata ctcacgcccc tgccagacaa aacggttgcc gctcacccgg
    20521 tttgcaaatg gcaccagcgg aaaacatgag gcatcggttg ccacaccgct ttttttacca
    20581 ggacgtaaga gcggcgtcgt atcgcgccag aagccttcga ttacgccgcc ctggtcagaa
    20641 acgtccagct ttagcgaccc gtgggataag gtatagatgg tcatccgcta actccttaat
    20701 ccgggaagtt aatcacaact ttcccgcatt tgccgctcgc catcagcaca taggcatcac
    20761 ctgcctgttc tagcgagaag cgatgggtaa tggcgttacg cggccacagc ttccagtcag
    20821 tcaaatcgtg ggcgcatttt tccatatgga acagactggt cacccaggag ccgataatcc
    20881 gccgttgatg gtgcatcaga tcagcgctga cctcgaattc cacttttccg gtttcaccaa
    20941 tgtaaaccac ccgcccccag tcagcagtgg attgcagtgc cagcaagcga cctgcggcat
    21001 taccggaaca atcgagcgca acatccgcgc caccgtgggt aagttcagca attatctgcg
    21061 gcagaccttc ggtggttgct aaatagccgt gatccatccc ccctaactgt tttgccatcg
    21121 ccagacgttc cggcagcata tcaacgccga tgatccgttt tgcaccgcga cctttcgcca
    21181 gcatcatcgc catcatgccg accggcccca gaccgaccac cagcacgtta tcgctgccgg
    21241 aaacttcgcc gcgcaaaatc ccttcatagg ccgtgccaac gccgcaactg ataaacgcac
    21301 catcttcgta gctcagcgca tccggcagga ggatcagatc tttttcttcc gccagcagat
    21361 attcggcatg accgccgtca cgctgccagc cgtaagccgc ttttcctttg ccagtacaag
    21421 agataggaaa gccgcggcgg cagttcgggc aaaaaccaca gccagaaatg tgatacacca
    21481 gcacgcggtc gccctcttta aaatggcggc agccttgccc catcgccaca atctgcccgc
    21541 acggttcatg accgttgata aatccctggt ataacggttt atcgggtgcc gccgccgtgg
    21601 cacggtgttg atgatagata tagtggacat cgcttccgca aatcccggag gatttcattt
    21661 tgatcagtac ctggttaatc cccggcgtcg gcaccgcaac ttcccgcaga tcgacggttg
    21721 aatttcctag taaataagca gccagcatcg ttttcataaa tccctcatta acaatacgat
    21781 taattttcat ccctgcagca cccgcgccag gggcgttaag gtttagcgtt tcgctttact
    21841 gcttcgctgg gtcagcaaga tattcgccag caccgccacc acgatgatga cgccgcgtac
    21901 cacctgctgg aaaaaggagt taataccgag cagcaccaga ccgttaccga ttagcgtaat
    21961 caccagcaca ccaagcaatg taccgaacag ggaaccgcga ccgccggaaa gtgccgtacc
    22021 gccgaccacg accgcggcga tgacgtcaaa ctccagaccg tttgcggcac ctgcgttacc
    22081 ggaaccgagg cgtgccgcca acaaaatgcc ggtcaccgcc gctaataatc ccgaaagggt
    22141 aaagataaga atgcgcactc gacgaacgtt aataccgcac aactgcgccg ccgtggcatt
    22201 accgccaacg gcaaaaaccg agcgcccgaa ggcggttttt cggctaatga acacaaacag
    22261 cgcaaacaac accatcatga tcagcgcgga tactggcaca ccgagaaatt gtccgcccag
    22321 ccagtccagc acctcgtttt cgtcaatcgg cactggcagt gcgttggtca taaacagccc
    22381 cattccgcgc agagcgctcc acagccccag tgtggcaacg aaacttggta cgttaaacac
    22441 gccgcgcagc accccggcga gcgttcccat cagcgcgcct aacagcaaca ccagcagaca
    22501 agcaatcgcc aacggaactt caaattgcag caaaaatgcc aggcacaccg agacaaaagc
    22561 caccatcggc ccaacgctga catcaatttc accggagata ataatcagcg tcatcgccca
    22621 ggcggcaatc ccaatggtgg cggcatcgcg cagcacgttc atctggttat tcaatgagat
    22681 aaagccaggc gcgttcaggg agaagaccag ataaagaatg gcaatcacca ccagcaaacc
    22741 gatctcatta atatggcgac tgacaaattg tttgagcgag acgctcttgc cctgcggcaa
    22801 tggtaatgac gaagcataca tcttcaggtt cctcggtaaa attcatcagt gcacagacag
    22861 aatggcggac atcagctcat ccacattgac cggcgagtga aactcctgcg agaacgtgcc
    22921 gtgctgtaat aacaggatgc ggtcacacac cagcggcaat tcctccactt cactggagat
    22981 aaacaccacg ctttttcctt cggcagccag ctcacggaca atacggtaaa tctgctgttt
    23041 ggcttcgata tcgacgccgc gcgttggctc gtcgagcaac aaaatctggc tggcggcata
    23101 gacccaacga ccgatcacca ctttttgctg attgccacca gaaagcgtgc cgatgggtgt
    23161 ttcgctactg gcagccttga ccgtcatccg ctgcatcacc tcttcggtca ggcggcggat
    23221 ggtggaccat tgcagcacac cgttggtgct gattttttgc cgattggtca gcactgtatt
    23281 ttcgtcaacg cctagccagg gaatgatccc cgcttctttg cggttttctg gcgtatagcc
    23341 aatgccgcgt ttcagcatgt cgccgtaatc ggggcgcatg attttctcgc cgttgataac
    23401 aatttcgccc tgttcatacg tctccagccc aacaatcgcc ttcagcaatt cactgcgccc
    23461 tgcccccagc aggccagcaa tgccaagcac ttcgccacga cgtagcgtaa agctgatatc
    23521 ctccagcttg ggcttatggc gtaacgcacg gacttccagt acggcctgat ccacaatttc
    23581 ctgaggggca accggcgcaa tatcaacgtg atcacgccct agcattagcg acacaatatg
    23641 atgcgtggag gtgttttcga gcatcacatc gcccgccacc tgaccatcgc gcataacggt
    23701 ggcataggag gcaatgcggc ggatttcttc catccggtgg ctgacataaa tcaccgccac
    23761 gcccagtgcc gacatctttt tcaccgcact gatcaccagt tcaacttctg cactggcaag
    23821 cgagctggtg ggttcatcaa gaatgaccac gcgcggctcg cccttcatca cccgtgcaat
    23881 ttccaccagt tgcttttgcg ccgggcttag cgttgaaaca agttgttcag gactaacgtc
    23941 aacgcccagc gcctgtaagc aacgttgggc atcctgcgcc atttgcaggt aatcaatcat
    24001 gccgttgcgg cgaggccatt gaccaaggca gaggttttct gccaccgtca gcccttccac
    24061 cagacttaat tcctgataaa ccgcacgaac ccccagttca gcggcacggc gagtcaacgt
    24121 agcttcgtca ccttccagtc gcgtctcgcc aatccagata tcaccgctat ccgggcgttc
    24181 gctgccggta agcattcgaa tgagagtcga tttgcccgcg ccgtttttac ctaacagcgc
    24241 acgaacttcg cctttattga gcgtgaagtt aacgttatcc agcgcaacga cgccggggta
    24301 acgcttattt cctgccacca cttttgctac cgggactgcc tctgttgccg tgaacatagg
    24361 gacctctgcg aatcagcgat tagggcagac catcaacgtg cgttgccagc cactgtttac
    24421 catcttccgt tttggtatag agatcgattg gcacctgaat cactttttca ccgtcggctt
    24481 gtttattgat aaccttcaat gtttgcgcga aaacagcatt gcccattttc ttaccggaaa
    24541 tatccactac cgctttcagc acctgattgt tttccagctc ctgagcaatt tcggttgtca
    24601 tatccgaacc gaaaacagca atttttccgg cctgattttg attacgtacc gcttttaccg
    24661 cgccgagtgt cgcaccgccc gactccccca taatggcgtt gagatccggc gtggagataa
    24721 tcagtttttc accaacggaa atcgctttat ctaaaacagt cccttcctgg ttagcgacaa
    24781 tttgcgcgcc gggaacgcgg gattttaata cttcttcaaa tcctttacgt cgctgcacac
    24841 aaacttcaaa ggcttcgcaa ttgatgacgg caattttcgg ctggtcaatt ttattggcaa
    24901 taaaataatc ggcggcagcg ttacccagtt tttaccaaat tccagtggat cgccgaccag
    24961 atacgccgag acatatttat cgacaccctt ttgattaata caggtgttgt agcaaatcac
    25021 cggaataccc gcttcactgg cgcgacgaac ggtacggcta ctgccatttt cagacactgc
    25081 cgataaaata atggcatcga cattacgcgc cacgagggta tcaacaaagg tactttcttt
    25141 cgaaatatcc ccctgggcgt tagtttcaat taactgtacc tgtactgaag aatcttttgc
    25201 cgcatcctga acgccctgac gcactccagc gtaatatccc tgggtatcaa ggtatattgc
    25261 accaatggtc atttctttat ccgcagccct ggcaaatagt gcgctaccta atagcgtagc
    25321 cattaataat agattacggg ttgttctcat ttttttaggc atagtgcctc ctgtagggtt
    25381 tttattaaca acggcttatt ctaattattt tgtgatgagc ggcagcgcgt gccgctcaaa
    25441 tattacagtt aatagggttt agtgaacgta gttaaaaata aaaggcatca cagtggtgaa
    25501 taatgccgct ttcggccagg ccggattaat cgccgtcaat tcagcgcgag cagactcata
    25561 ttgcgcaacc tccccgagtc ccgcggacgc cagcatcgcc accatcaggc atttttcttt
    25621 atgctgctgt tgtaaatcgc catacagcga taacaggtca ggttgtgaaa cagcaaagaa
    25681 atccgcctca atactggttt tcgccatctc ttgcgcccac tgtttcattt cactaaacag
    25741 ttgctgtgcg gtttgctgtt cacccagcag tcgcagcgcc attccttgcc agaagagata
    25801 atcaaccggc tgatcgttgt aataactgtg gatgttaatg gtgcgatcgc cggtcgccgc
    25861 cagacgtaaa caacgcatcg cttcagtttc atcgccctgc gcgttggcgc atatcgcctg
    25921 ccagaaccag atgtcgttat cagtttgccc cggtaaacgg ccttcgctta aattctccgg
    25981 ataatacagc gcggcatgaa gcagttcgct ggcctgctgc ggctctctgg catcaagatg
    26041 ctgccaggcg cgtaataact ggttgaggat aaactgactg gtgaccttcc cttccccgcc
    26101 ttcccacggg tggaatttac gcgtggcgag aatgtccgcc gctttgtctg cctgacccgt
    26161 gagatgccac aaattgagca gttctgcggt catgtcgtcg cgtttcagcg caatttccag
    26221 attattttcc agacgcgcca gtcgtttctc cggtgtggct ccacttaact tatccagcaa
    26281 atcccgttcg aaaagcagac gtgcatcctg cggcgcaagc tgataagcat tatcaagata
    26341 acgcgcggcc agctcataat cgtgtttctt attccacgca tggatcgcta acccgcgcca
    26401 gccgtcggca aactccggcg acatctctac gcaacgttgc cataaggcaa tggctttgtt
    26461 gtagctacgt ttgttgtagt agaagcaggc cagtaaatgg cgagcaaacc aacactcttc
    26521 aatactctcc agcgccgcca cttcttccag cgtattcggg aaacggacaa actgcgggaa
    26581 gacatcaatg gcttttgcga ccagttcgcc acgttcggct ttcggcagca agctggcttg
    26641 caggtaaagc ggcagcgtgc gctggcagtc cagtgcgttc agcatctctg ccgccagggt
    26701 gggcattccc cagttaatca gttgcccggc ggtcatcaga gcgttaacgt cgcgtccctg
    26761 acacagaccg cgccactgcg cgagggctga ttcgctacga ccatcgaacc agttcagcca
    26821 ccacagagtg gcgttcagcg gataatcacg cagcagtttc tcgcgctgca aacgcgcgtt
    26881 gtcctgacga ccacttaaca ccagcagcag attatgcaag caaagcactt cctgattggt
    26941 tgggcaggca cgaagacttt gttggcaaaa atccagaccc gcgtcgaagt tatcattacg
    27001 cgccgccagt cgtgccagac cataatagcc gcctgcttta ctattgccgc tccagaccgc
    27061 acgccagaaa tcctcttcgg cttgttgata ttgtccctga cgttcgtaag cactggcgcg
    27121 aatcaaactt gcttgtccac actgcggttt tttgttcagc gcatgtgcac gtttcagcgc
    27181 ctgactggca tacgccaccg cttgcgggaa atctgcgcgg ttatattcca gcatcgccag
    27241 cgccaggtta cagcgataat ccagcgggtc cagcgccacg ccgcgcaggt agtaatcgaa
    27301 cggtgaacgg ctggcgtgat gatattgctc cagatgctga ccgataaacc aggcttcatc
    27361 tgtactggta atgtcttgtg ctgccagtgg cgctttggcg acgtccggca acggcaactc
    27421 ctgcggctga tgttcctgat aactcagtac aatattgccg tcggcatcgg agagttcaat
    27481 ggtcagtctg tcaggattga taccgtgcaa cacgccctgg atggcggtcg ctggtgtcag
    27541 ggccacggca tcatcgagca acgcgttgca tttgccgatt tcgcggatcg ccagacggta
    27601 tccgttcaac ggagagatgg catacagccc ccactcaatc ccccgctcac tacgctggag
    27661 ttttatcacc gcatcgcggg aggcattttg caccatgccc agagaatgat aaggcaggaa
    27721 atactgctcg aaacgcttct cttcgtaagc atcaagccag gtaaaatcag gctggttatc
    27781 ggcaaaaata ccggtcatca gttcgatata cgggccgtta ttgtcggtca gactcttatc
    27841 ccacgcctgg ccaaattcac tgtgtcccca actccactgt tttttacctg gcgcaatatg
    27901 gtggttggca acgtgcagca aaccgccatc ttcatcgtga caccacgcgc cgacaaaatc
    27961 gtactgcgat ttttcggcca tatatgaggt tggaacaggc acatttttat agcgagaaat
    28021 gtccactccg gcggagtagt ccactttgta gtaagtgccg gtggcgatgg ggaaagcgga
    28081 gacggcccgt ttgccgtgat caaacaccgc agtcacatcc ggtgggaaaa cgctctgatg
    28141 cccttcaccc cctttcactg ccgggttgac ccaccacaag aaatgacgcg gcgtggcgtt
    28201 gccgttatag acgcggctgg cgatttccag tgccgcccgg tcagggcgca gggtgaaacc
    28261 tgtcatcacc tgtaaaccat gcatcggctc cgtttcgccg acccacaccg tctgtgcacc
    28321 gtcttcatgg gcttcgaggg tgaaatcaac gggcataaag gtggtcgggc gatggtgttg
    28381 cggccagtta aactcaatcc cgccggagat ccacggtccc agcatcccca ccagcgcagg
    28441 tttaatgact tcattgtgat agacaaaatc gcgctgtttc actttatccc atgcgcgatg
    28501 cacccgaccg cccagttccg gcaggatcat cactttgatg tagtcgtttt ccagccacac
    28561 cgcctgctag gatttcaggg ttttctgctc gctcagcgta tcggtcacgc cgtagggata
    28621 aaccgcgccg gacgatccct gataaacgcg attttccagg aacatgggat gtatatcctg
    28681 cggcccggtt tcataggtcg ggatctcaac gcgctcttgc cacactttta ctggagtcat
    28741 ggtgccctca atattaacaa gacatactga attaaaagat ttgtggcagt gtattgaaca
    28801 atctggcaat gttttcgcgg aataatcacg caattaacta aacaaggttt agtgaagatg
    28861 agagcctgca ttaacaatca acagattcgc caccataaca aatgcgtgat tctggaactg
    28921 ctgtaccggc aaaagcgcgc caataaatca acgctggccc ggctggcgca aatttcgatt
    28981 ccggcagtca gtaatatttt gcaggaactg gaaagcgaaa aacgggtggt gaatattgac
    29041 gatgaaagcc agacgcgcgg gcatagtagc ggtacatggc tgattgcgcc ggaaggtgac
    29101 tggacgctgt gcctgaacgt gacgcccacc agtattgagt gtcaggttgc taatgcttgt
    29161 ttaagtccga aaggcgaatt tgagtattta cagattgatg taccgacgcc gcaggcgctg
    29221 ctgtccgaaa tcgaaaaatg ctggcatcgc caccgtaaat tgtggccgga ccgcaccatc
    29281 aatctggcgc tggcaatcca cgggcaggtt gatccggtga ccggcgtatc gcaaaccatg
    29341 ccgcaagcgc cgtggacaac gccagttgag gtgaagtatc tgctggaaga gaagctgggc
    29401 attcgggtgc tggtcgataa tgactgcgtg atgctggcgc tggcggagaa atggcaaaat
    29461 aattcgcagg aacgggattt ctgcgtgatc aacgttgatt acggcattgg ctcgtcgttc
    29521 gtgattaacg agcaaattta tcgcggcagt ttgtatggta gcggacagat tggtcacacc
    29581 atcgttaatc cggatggagt cgtctgcgac tgtggacgtt acggcagcct ggaaaccgtc
    29641 gcctcgttaa gtgcattaaa aaaacaggcg cgggtatggc taaaatcaca accggttaat
    29701 actcagcttg agcctgaaaa actgacaacc gcgcagttaa tcgctgcctg gcagagtgga
    29761 gaaccgtgga tcaccagctg ggttgatcgc tctgccaatg ccattggttt gagtctgtat
    29821 aacttcctca acatcctcaa tattaatcag atttggttgt acggtcgcag ttgtgccttt
    29881 ggtgagaact ggcttaatac tattattcgc cagacaggat ttaacccgtt cgaccgcgac
    29941 gaaggaccga gcgtgaaagc gacgcaaatt ggcttcgggc aattaagccg cgcacaacag
    30001 gtgctgggaa ttggctattt gtatgttgag gcgcagttac gacagatttg atggcgcgat
    30061 aacgtagaaa ggcttcccga aggaagcctt gatgatcata aacgaaaaat tgcctgatgc
    30121 gctacgctta tcaggcctac acggagattg caatatattg aatttgcaag attttgtagg
    30181 cctgagaagg tgttcatgcc gcatccaaca tgaacaacga gcacattgtc agcaaatcac
    30241 cgtttcgctt atgcgtaaac cgggtaacgt gcgcagatgt cgagaacttt acctttgatg
    30301 cgctcgataa cggcttcatc attgatgctg tccagcacgt cacacatcca gccagccagt
    30361 tctttcgctt cggcttcttt aaagccgcga cgggtaatcg ccggagtacc tacacgaata
    30421 ccggaggtca caaacgggct cttcggatcg ttcggtacgc tgtttttgtt gacggtgatg
    30481 ttagcacggc ccagagcggc gtctgcttct ttaccggtca ggtttttatc aaccagatca
    30541 accaggaaca ggtggttatc agtgccgccg gaaaccactt tgtagccgcg ctcgaggaac
    30601 acttctacca tcgctttagc gtttttcgcg acctgctgct ggtaagtttt gaactcaggc
    30661 tccatcgctt ctttcagagc aaccgcttta ccggcgatta cgtgcatcaa cggaccgccc
    30721 tgaccaccag ggaaaacggc agagttcagt tttttgtaca gctcttcgct accacctttc
    30781 gccaagatca ggccgccgcg cggacccgcc agggttttgt gagtggtggt agtaacaacg
    30841 tgagcatgag gaaccgggtt cgggtagacg ccagcagcaa ccaggcccgc aacgtgcgcc
    30901 atatcaacga acagataagc accgatgctg tcagcgattt cacgcatttt cgcccagtca
    30961 accacgccgg aatatgcaga gaaaccaccg ataatcattt tcggcttgtg ttctttggct
    31021 tgtttttcca gatcggcgta atcgatatga ccggtagcat cgataccgta aggaacgatg
    31081 ttgtacagtt taccggagaa gttaaccgga gaaccgtgag tcaggtgacc gccatgcgcc
    31141 aggttcatac ccagaacggt atcacctggt tccagcagcg cggtgtagac cgcaaagtta
    31201 gcctgggagc cggagtgcgg ctggacgtta gcgtagtcag cgccgaacag ttctttcgca
    31261 cgatcgatcg ccagttgttc aacgatatca acatactcgc aaccgccgta gtagcgtttg
    31321 cccggataac cttcagcata tttgttggtc agctgagaac cctgcgcctg cattacgcgc
    31381 gggctggtgt agttttcgga ggcgatcagt tcgatgtgct cttcctgacg tactttttcc
    31441 tgctccatag cctgccacag ttcggcatca taatcggcaa tgttcatttc acgctttaac
    31501 atccgcatct cctgactcag ctaacaataa aatttttggc ctttataggc ggtcctgttg
    31561 gacaacggcg aacagtataa ccgaatcatt gtgcgataac aggtcttgac aaaggaattt
    31621 acgcaaacga ttaccttcag gctacgcaag gctttggaga ataaagagct tgcaaccgga
    31681 aacggatttc ttttcaggtt tgtgatgcaa atttttcact tcatcacatt ctttctgaaa
    31741 aacaccaaag aaccatttac attgcagggc tattttttat aagatgcatt tgagatacat
    31801 caattaagat gcaaaaaagg aagaccatat gcttgacgct caaaccatcg ctacagtaaa
    31861 agccaccatc cctttactgg tggaaacggg gccaaagtta accgcccatt tctacgaccg
    31921 tatgtttact cataacccag aactcaaaga aatttttaac atgagtaacc agcgtaatgg
    31981 cgatcaacgt gaagccctgt ttaacgctat tgccgcctac gccagtaata ttgaaaacct
    32041 gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag cacaccagct tccagatcaa
    32101 accggaacag tacaacatcg tcggtgaaca cctgttggca acgctggacg aaatgttcag
    32161 cccggggcag gaagtgctgg acgcgtgggg taaagcctat ggtgtactgg ctaatgtatt
    32221 tatcaatcgc gaggcggaaa tctataacga aaacgccagc aaagccggtg gttgggaagg
    32281 tactcgcgat ttccgcattg tggctaaaac accgcgcagc gcgcttatca ccagcttcga
    32341 actggagccg gtcgacggtg gcgcagtggc agaataccgt ccggggcaat atctcggcgt
    32401 ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt cagtactctt tgactcgcaa
    32461 accggatggc aaaggctatc gtatcgcggt gaaacgcgaa gagggtgggc aggtatccaa
    32521 ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa ctggtcgctc cggcaggtga
    32581 tttctttatg gctgtcgcag atgacacacc agtgacgtta atctctaccg gtgttggtca
    32641 aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca ggccacacag cacaagtgaa
    32701 ctggttccat gcggcagaaa atggcgaagt tcacgccttt gctgatgaag ttaaggaact
    32761 ggggcagtca ctgccgcgct ttaccgcgca cacctggtat cgtcagccga gcgaagccga
    32821 tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat ttgagcaaac tggaaggtgc
    32881 gttcagcgat ccgacaatgc agttctatct ctgcggcccg gttggcttca tgcagtttac
    32941 cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac attcattacg aatgctttgg
    33001 cccgcataag gtgctgtaat ttgatgttgc cggatggaaa catccggcaa cccttgacgc
    33061 ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg cgaatgaccc gagccacgtc
    33121 aaagacgaag attttaccgt caccgatttt gccggtttgc gccgtgcgaa taatggtatc
    33181 gacacaggta tcgacaatgt cgtccggtac gacaatctca attttcactt tcggcagaaa
    33241 atccaccata tactccgcgc cgcggtacag ctcggtatgg cctttctggc gaccaaagcc
    33301 tttcacttcg gtcaccgtca tgccggtaat accgacttcg gccagtgctt cgcggacatc
    33361 gtccagcttg aagggtttta taatcgcatc aatctttttc atgctattcc ttgaaaaggt
    33421 tgcctgtctt ttgatctgct aaacgtaaca cataacgcca attcattcct tgaaatcgtt
    33481 tgcatccagc tcgtgtcggg aaagcagttt ataaaattct gtccggttgc gccccgccat
    33541 tctcgccgcg tgggtgacgt tgcctttggt gatttgcagc agcttacgca agtagttgag
    33601 ttcaaaatga ttacgtgcct caacaaaggt tggcagcgcc gtattttcac cctccagcgc
    33661 ctgctccacc agcgcatcac taatcaccgg agatgaggtc agcgccacgc actgttcaat
    33721 cacgttgacc aactggcgca cattacccgg ccagctcgcg gtcatcaggc gtttcatcgc
    33781 atcggtagag aacgcgcgga caaacggttt atgtcgctct gccgcctggc gcaacaggtg
    33841 atttgccagt agcggaatgt cttctgtgcg ctccgccagt gccggaattt tcaggctgac
    33901 aacgttgagg cggtaataga gatcttcgcg gaattccccg cgcgccatcg cttttgacag
    33961 atcacggtga gtggcagaaa taatccgcac attgatatca atatcgcggt tactgcccag
    34021 cgggcgcact ttacgctcct gcaacacgcg cagcagtttg acctgtaacg gcgcaggcat
    34081 atcgccaatt tcatcgagaa atagcgttcc gccttccgcc gcctggaata aaccttcgcg
    34141 attgctgaca gcgccagtaa acgcgccacg cgcatgacca aacagctccg actccagcaa
    34201 ttgctcgggc aatgcgccac agttaatagc aataaatggt ttgctgttgc gcgggctggc
    34261 gttgtggata gcctgggcga aaatctcttt cccggtgccg ctctgaccgt taatcaaaac
    34321 gctgacgtct gattgcgcca ccagccgcgc ctgttccagc aaacgcagca tcagcgggct
    34381 gcgggtgaca attgcctcgc gccagcgttc atcggtggct ggcgcggatt gctccagcgc
    34441 atcgtcaatt gcctgatata gcgcatcttt atcgacaggc ttggtgagga aactaaaaac
    34501 gccctgctgt gttgcagcaa cggcatcggg gatagaacca tgcgcagtaa gaataattac
    34561 tggcattccc ggctgcactt tctggatttc agcaaatagc tgcataccgt ccatttcatc
    34621 catccgcagg tcgctgataa ctaaatctac tttttcgcga ttcagtaccc gtaatccttc
    34681 agcgccactt tccgccgtga ccacactgta gccttcgctg gtcaggcgca ggccaagcag
    34741 tttcagcaat cccggatcgt catcgaccaa taataaatgc gcaggtttat ggctcatcag
    34801 gagtgacctc atgggtggat ggcgcgggct tttcactctc gtgcggcgta tccggcgaga
    34861 aatttccagc cggtttgcgg gtcgagagct ggcgttcaat atcggtcagg ttttccagct
    34921 tgcgggtggt gagttccagt tgctgctgta gaacgtgatg ttgctggcgc aatgtatcca
    34981 gctcgctgtc gctggactgc tggagtttac tgtaacgttg gcgctcttcc gccagttgca
    35041 gttgcagcgc ctgaccgtcg cgccagagtt gatacagtgg gcgaacctgt gccgggatct
    35101 cggtacttaa cgcttcaata cgcgcgacca gctggcggcg ctcatacggc gtaattttgg
    35161 cgtcggcgag caaaatccct tgtttaaagg tattttgcca gctgccgtcg tcatattggc
    35221 gagcttgctg acgcgactgc gcaggcatta aacgatcagc acaatccatc gcccgcagcc
    35281 agtaaagcgg attggtttcg gttgatttac cttgcagcgc ccagatgtcg ctacattcag
    35341 tagaaagata gtcagccagt tgataaaccg gaattttttc ttctgctggc gtatcaatgg
    35401 ctggcttatt gtgattctgc acgcaaccca gcaatgccag acatggcagc cctgccagcc
    35461 acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat attcaccaga cttaaagcct
    35521 atcccagtgg gcgtaattgt tgcagacagt ctggacatgg acagcgcgga gaaaccgggg
    35581 cgtacatatc gtacgtgaag atttcgagca ctacccgggg ccgaactgac aaataaaata
    35641 gcctgatagg ataggctgtt agcattattt cgtgtttttc gacgacggta attcaatgcg
    35701 gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt tccccttgca tacggcgaat
    35761 acaatccctg gcaatgctta atcccagacc gctgcccttc accgcccctt ttcgctggtg
    35821 gcttccctga aaaaagggtt cgaagatcat ggcgcgttcc tcttgcggaa tgggcgtgcc
    35881 tgtattgatg acatcaatat aaacccgcgc accatgtaaa ctgctgcgaa ggcaaatgtt
    35941 accggattca gccccgtagt gcaccgcatt ggagtaaaga ttatccagta cgctcatcag
    36001 cagcattggc tccgccaggc aagctgttgc tttgagatcg acgtcggtat gcatcatttt
    36061 agcccgtgcg ggcaggctat gagcagaaac cactgtctcc accagcggtg ctaattcaac
    36121 attctccagt tccaccgcac tgtccgcctg tttacggttg taatcaagca gttgttcgat
    36181 cagtttttgc aaattgcagc tgctgctatc aagaatgctc accacctctt tttgctctgg
    36241 cgtaagcggc ccgacaacct gatcagccag taattcagtg ccctcgcgca tactcgccag
    36301 tggcgttttt aattcatgag ataaatgtct taaaaattga tggcgttggg attccagcca
    36361 tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc gagcgtaact cgctcggtcc
    36421 acagaacgaa acgctattgc ccagagaacg cccttccccc agacggttga tcatgcgctc
    36481 gatatttttc accggcccga taatcatccg cgtgaaaagt agcaccatca ccagactcac
    36541 cagaaatagc accagcgatt gccaaccaaa atattgccca cgttcggcga tttcacgctg
    36601 aagttgctgc ccacgagaga acaccactgt gcgcgtggcc tgcaccattt cggtattggc
    36661 actggcaaag gcttcaagac gcgcggcggc ggcagcatcg ggaccactgt tgttacactg
    36721 aagttgggcc agattgttca aatcctgacg caatgcctgg taaagtttat cgtctggcag
    36781 tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg cgctggcttt gataaacctt
    36841 cgcaagcgta gggtcgtcca gcacgcaata ctgacggtag ctacgctcca tctccagcgc
    36901 cgcgttggtc atcgcttcac tacgccgggc atcaataagc gtagtgcggt taaccagcgc
    36961 cgcctgatcg ctaagcgcat tcaggctttg ccaggcttgc catgccagca ccaacagggg
    37021 tagtagaatt agcaaaaatg ccagcattac cagttgtcgt aatgagcggg gaaaaacggg
    37081 ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc ctgatgctaa ccgaggggaa
    37141 gttcagatac aacaaagccg ggaattaccc ggctttgtta tggaataagg cggtgcctaa
    37201 ctcgacgttt cgcccgatgg ttgatatagc tacgctgata tcagaagttg gacggcaggc
    37261 accttgttgt gcgtcattcg aattttatgt agcacgtccc gaaggggctg acataagtcg
    37321 gtgaatgagc cactgcttac tattatgcag taactgtgcc aataaagaaa atagtttggt
    37381 aacgtactga ttatacgttg ctttgaggga tttatggctt cttcgctatt tgaatgtaaa
    37441 tcagccactg tgtcgctaaa aagagacaac ttaagataaa cttattagat tgcaataaaa
    37501 tcaatcattt aagtgtcgcc agaaagcgac acggtaaacc acccattgtc gtgatttaca
    37561 gacacaaaaa agctcccgga gttgggagct tatgatagtg gttggtgctt aatgccgcat
    37621 ccgggctgca aaaccaatga actgatgact taccccaact gcttacgcgc attgcggaaa
    37681 atgcgcatcc atgggccatc ctcgccccag ttttccggat gccaggagtt gctgacagta
    37741 cggaaaacac gttccgggtg cggcatcata atggtgactc gaccactttc agtcgtaacc
    37801 gccgtaatac cgttcggtga accgttcggg ttagccgggt aggtttcagt gactttgccg
    37861 aagttatcga catagcgcag tgccaccagc cctttgcttt ccagtgccgc cagatgcgcc
    37921 gcatcacgca cttccacgcg cccttcacca tgagagacag caatcggcat ttgcgagccc
    37981 accatcccct gcaacagcag agacgggctt tgggttactt caaccaggct gaaacgcgct
    38041 tcaaagcgat cggaggtgtt acgcacaaaa cgtggccaca actcactccc cgggatcagt
    38101 tcacgcaggt tagacatcat ctggcaaccg ttacataccc ccagggccag cgtttgcgga
    38161 cgatggaaga aggttgcaaa ctcatcgcgt acacggtcat tgaacaggat agacttcgcc
    38221 caaccttcac cggcacccag cacatcaccg taggagaaac ctccgcacgc gaccagggcg
    38281 tgaaaatcct ccagtcccgt acgtccagtc agcaggtcgc tcatatgcac gtcgatagca
    38341 tcaaagcccc cacgttggaa agctgccgcc atttcaacat gcgagttcac gccctgctca
    38401 cgcagtacgg caactttcgg acgtgcgcca gtggcaatat acggtgctgc cacatcttcg
    38461 ttgatatcga acgacagttt tacattcagg cccggatcgg cgtcgttaga tttcgcctga
    38521 tgctcctgat cggcacactc cgggttgtca cgcaggcgct gcatctgcca ggtagtttct
    38581 gcccaccaga cacgcaacgt agtgcggctt tcgctgaata cagtctgccc gttggcggta
    38641 atcacaaaac ggtcaccgga aaccgcctgc ccaacataat ggacgcaatc cgcaagccca
    38701 tgctgtgcca gtacggactc gaccgcttca cggtcagcgg cacgaacctg aatcaccgca
    38761 cccagttctt cgttaaacaa cgccgccagg cgatcgtcac ccagagaggc gatatccgcg
    38821 tcaatgccac aatgacctgc aaaggccatt tccgccagcg ttaccagcag gcctccatca
    38881 gagcggtcgt gatacgccag cagcttacgc tgtgcaacca gcgcctgaat cgcgtcatag
    38941 aagcctttca gttggacaac atcgcgtaca tctgccggtt tgtcgccaag ctgacgataa
    39001 acctgcgcca gcgccgttgc gcccagcgcg ttattgcctt tgcccaaatc aatcagcagc
    39061 agtgcgttat cttcggtaca aagctgcggc gtgatggtgt gacgtacatc ttccacgcgg
    39121 gcaaatgcag aaatcaccag cgacagcggc gacgtcattt cgcgctcttc gttaccttcc
    39181 tgccagcggg ttttcatcga catagagtct ttacccaccg ggatcgtcag gcccagcgcc
    39241 ggacaaagtt cttcacccac ggctttaacg gcttcataca ggcccgcatc ttcaccaggg
    39301 tggcctgccg ccgccatcca gttggcggaa agtttaatgc gtttgatatc gccaatctgt
    39361 gttgcggcaa tattggttaa cgcttcaccg accgccagac gggcagaggc ggcgaaatcc
    39421 agcagcgcaa ccggcgcacg ctcgccaatc gccatcgctt caccgtagta gctgtcgagg
    39481 ctggcggtag tgaccgcgca gttagcgacc ggcacctgcc acggccccac catctgatcg
    39541 cgcgccacca tgccggttac gctgcggtca ccgatggtca ccaggaaagt tttctccgcc
    39601 acggtcggca gatgcagtac gcgtttcacc gcgtctgcaa tggtgatccc ttcacggacc
    39661 agcgcatcgc ccttcgcttt cagcgtttgt acatcgcggg tcatcttcgg cgttttacca
    39721 agcaggacgt ccagcggcag atcgatcggc tgattatcaa aatgacgatc gtgcagagaa
    39781 agatgcagtt cttcggtcgc ttcaccaatc accgcgtagg gtgcgcgctc acgcttacac
    39841 agttcgtcaa acagcggtaa ttgatcggca gcaaccgcca gcacgtagcg ttcctgggat
    39901 tcgttacacc agatttccag cgggctcatg cccggttcgt cgcttagaat atcgcgcagt
    39961 tcaaatttac cgccgcgtcc gccgtcgctc accagctccg gcatcgcgtt agaaagaccg
    40021 cccgcaccga cgtcgtggat aaacagaatt gggttagcat caccaagctg ccagcaacgg
    40081 tcgatcactt cctggcaacg acgctccatc tccgggttgt cgcgctgtac ggaagcaaag
    40141 tcgaggtcgg catcagactg accagacgcc atggaagacg ccgcgccgcc gccaagaccg
    40201 atgttcattg ccgggccgcc gagaacgacc agcttcgcac cgacgttgat ctcgcctttt
    40261 tgtacgtgat cggcgcgaat gttgccgatc ccgcccgcca gcatgatcgg tttgtgataa
    40321 ccgcgcagct cttcgccatt gtggctgttc actttttctt cataagtacg gaagtagccg
    40381 ttcagtgccg gacgaccaaa ttcgttgtta aacgccgcac cgcccagcgg gccttcggtc
    40441 atgatgtcca gcgcggtaac aatgcgctca ggcttaccga aatcttcttc ccacggctgt
    40501 tcgaagccag gaattcgcag gttggatacg gagaaaccaa ccaaaccagc tttcggcttc
    40561 gcgccgcgcc cggtggcacc ttcatcgcgg atttcaccgc cggaaccggt cgccgcgccc
    40621 ggccacggag aaatcgccgt cgggtggttg tgagtttcga ctttcatcag gatgtgcgcc
    40681 ggttcctgat ggaaattgta gcggcccgtt tcgtggtcag caaagtagcg gcccacttca
    40741 gaaccttcca ttacggcggc gttatcttta taagcagaga gaacgtgatc tggcgtggtt
    40801 tcgaaagtat ttttgatcat cctgaacagc gatttcggct gttgttcacc gtcgataatc
    40861 cagtcggcgt taaaaatttt gtggcggcag tgctcagagt tcgcctgagc gaacatatac
    40921 agctcgatgt cgttcgggtt acgaccaagc tttgtgaaag catcctgcag atagtcaatt
    40981 tcatcttccg ccagagccaa gccaagacgc aggttagcgt cgatcagcgc ctgacggccc
    41041 tgccccagca aatcaacgct tgtgaccgga gtcggttgat ggtgggcaaa caactgctct
    41101 gcatcatcta aatcaaaaaa gaccgtttcc atcatgcggt cgtgcagttc agcggtaacc
    41161 tgctgccatt gttcattggt cagcgtaccg gcttctatat agtaagcaac gccgcgctca
    41221 aggcggttta cctgttgtag cccgcagtta tgggcaatat cggtcgcttt cgaagaccag
    41281 ggagagatgg tgccaggacg cggggtcacc agcaggagtt tgccttgcgg ggcgtggctg
    41341 gcgagtgccg gaccatattt cagcaggcgt tcaagttgtg cgtgctcatc atcgtttaac
    41401 ggcgcattga ggtcagcaaa atggacatac tcggcgtaaa tattgtgaac cgggagcctg
    41461 gcagcctgaa aacgtgccag cagtttgttg attcggaatg ccgacagtgc aggcgaacca
    41521 cgcagaattt ccatcataag tctctcgtct tctaagcttt cggtgtaccc aagggggggg
    41581 aaacgggcgt cattataaag aatctgatgc gctgacgaaa ccgtttgcgt ggaaataaaa
    41641 tcaccatcgt gaattagcaa cgcgtgccgc caatggctgt aataagttgc catctggcgc
    41701 aggtttacgc aaaatgccgc tcatttatga gtaaaccttt cactattatt acgttttttc
    41761 aagctgggac gcgcacgaca cagagaatta actaattgaa aaaattaaag attaattatc
    41821 tgttcatcgg cattctggca ctgctgctcg cggtcgctct ctggccatcc attccctggt
    41881 ttggtaaagc cgacaaccgt atcgccgcca ttcaagcgcg gggagagttg cgtgtgagca
    41941 ccattcatac tcccctgact tataacgaaa tcaacgggaa accttttggc ctggattacg
    42001 aactggcgaa acagtttgcc gattacctcg gcgtaaaact gaaagtgacc gtgcggcaga
    42061 atatcagcca gctgtttgac gaccttgata atggtaacgc cgacctgctg gcggcaggac
    42121 tggtctataa cagtgagcgg gtaaaaaatt atcagcctgg ccctacctat tattccgtgt
    42181 cacaacaact ggtttataaa gtgggtcagt atcgcccacg tacgctgggc aacctgacgg
    42241 cggagcaact caccgttgca ccgggtcatg tggtagttaa cgatctccag accctgaaag
    42301 aaacaaaatt cccggaatta agctggaagg tagacgacaa aaaaggctct gcggaattaa
    42361 tggaagatgt catcgaagga aaactcgatt acaccattgc tgattctgtc gccatcagcc
    42421 tgtttcagcg cgttcacccg gagctcgccg tagcgctcga tatcaccgat gaacaaccgg
    42481 tgacttggtt tagcccgtta gatggcgata ataccctttc cgccgccctg ctcgacttct
    42541 tcaacgaaat gaatgaagac ggtacgctgg cacgcattga agagaaatac ctggggcatg
    42601 gcgatgattt tgattacgtc gatacgcgca catttttacg cgccgtcgat gcggtactgc
    42661 cgcagttaaa gcccctgttt gagaaatacg ccgaagaaat tgactggcgt ttgctggccg
    42721 ctattgctta tcaggaatcg cactgggatg cacaggccac ttcaccgacg ggtgtgcgcg
    42781 gcatgatgat gttaaccaaa aataccgcgc aaagcctcgg cattacggat cgtaccgatg
    42841 ccgaacagag catcagcggt ggcgtgcgtt atttgcagga tatgatgagt aaagtgccgg
    42901 aaagtgtgcc ggagaacgaa cggatctggt ttgccctcgc tgcgtacaat atgggctatg
    42961 cgcatatgct ggatgcccgc gccctgacgg caaaaaccaa agggaatcct gacagttggg
    43021 ctgacgtaaa acagcgtctg cctttactta gccagaaacc ctattacagc aagctgactt
    43081 acggctacgc tcgtgggcat gaagcctacg cttatgtcga aaatattcgt aagtatcaga
    43141 ttagcctggt gggttatctg caagagaaag agaagcaggc tacagaagcg gcgatgcaac
    43201 tggcgcagga ttatccggcg gtatcgccta cggagttggg caaagagaaa tttccttttc
    43261 tctcgtttct ttcccagtcg tcatcaaact atttgaccca ttctccctct ctgctgtttt
    43321 ccaggaaagg gagtgaagag aaacaaaatt aatccgtcga ggattgcgct tttttctgcg
    43381 ctttaatttc ctggcggcgc atgcgaaaga agtcactgag caacgccgcg cactcatccg
    43441 ccagtattcc ttccgtaatt tccactcggt gattcatacc cggatgatgc agcacatcca
    43501 ttaaagatcc cgcagcgcca gttttcgcgt cacgcgcacc aaagaccacg cgaccaatgc
    43561 gactgtggat catcgctccg gcacacatta cacatggttc aagcgtgaca tacaacgtgg
    43621 cgtcgatcag acgataattt tgcatcacca gaccaccctg ccgcagggcc atgatttctg
    43681 catgtgcggt gggatcatgg cgaccaatcg ggcggttcca gccttcgccg attacccgat
    43741 tgttatgcac taataccgcg ccgaccggca cttcccgctc atcccaggca cgtttcgcca
    43801 gcgtcagcgc gtgacgcatc caatattcgt ggctaaattc gacttcagac aaaaagaaaa
    43861 ctccggttat aaaagcgcgg cgcattatac acggacgcta tgctttactc tattccagtt
    43921 gctggagttc accgcgcggg gttactcgcc aacgatgctg acagaaataa agcagcggat
    43981 tgtcctgatt actgtcgcta tagccactgt acagccgcag cggcgtgccg attttgcgct
    44041 ccagttgtgc gaccttttca tgtcccagac aacgcatcgt caatacccaa ccaccatagc
    44101 cacgctgaat ttggctggcg ataagattaa cccgcggcag ccagggcgta tcgaaataaa
    44161 ccgcttcaac cagcggctgc ggagagccgg taatcaacca gatatcagca tcggaactta
    44221 acaggtaggt ggttaatcgc tcctgaacca gcggaaaggc ggtaacattg tcgcgaaacc
    44281 agcgcacgaa atcggcctgc aacgtctgta aacgtgcttc gctgtgacca aaagtgcacc
    44341 cccacagaag cagactcatc ggccagcgtg ccgcacgacc ttttatcaat aacgcaatgg
    44401 ctataatcgg taacaacggc aggacaagta acgcattcag cggttggcga cgcagtaaat
    44461 agcgcagaaa actgccgaac atatcctgct gatgcaatgt tccatctaag tcaaaaaaca
    44521 ccacacgacg ctcgtgagtt gccaaaccat actcctctgg actgaaacat cctgattaat
    44581 tttcgctact acatagccta acagatagat catcactttt ccggcaacaa tcgaccgcat
    44641 catggctaac tggaattttt aattcatggc aattagcggc aatggaatat aaaattcact
    44701 cgcgtgtgtc tcatattgcc tgttgtcgcc actatttcat cgcgaggaaa gatatgaacg
    44761 gcttacttcg tatccgtcag cgttaccaag ggcttgccca gagtgataaa aaactggcgg
    44821 attatctgct gctacaacct gatacggcgc gccatttaag ctctcagcaa ctggccaacg
    44881 aagccggagt cagtcagtcc agcgtcgtga agttcgcgca aaaactcggc tataaaggtt
    44941 ttccggcgct taagttggcg ttgagtgaag cgctggcaag ccagccggaa tcgccctccg
    45001 tgcccattca taaccaaatc cgcggtgatg atccgttacg gctggtcggc gaaaaactga
    45061 ttaaagaaaa taccgccgct atgtacgcaa cgctaaacgt taatagtgaa gagaaactgc
    45121 atgaatgcgt aacaatgttg cgctctgcgc ggcggataat tctgaccggt attggcgctt
    45181 cgggtctggt ggcgcaaaac tttgcctgga agctgatgaa gattggcttc aatgctgccg
    45241 cactgcgcga tatgcatgcg ctactcgcaa cagtacaggc gtcgtcccct gacgatctgt
    45301 tattagccat ttcctacacc ggtgtacgac gcgagttaaa cctggcggca gatgagatgc
    45361 tgcgagtggg cggaaaagtg ctggcgatta ccggctttac tccgaatgcc ctgcaacagc
    45421 gtgcttctca ttgcctgtat accattgccg aagaacaagc gacaaacagt gcttcaatct
    45481 ctgcttgtca cgctcaggga atgttaacgg atttgctgtt cattgcgctg attcagcagg
    45541 atctggaact ggcaccagaa cgtattcgtc atagtgaagc gctggtgaaa aaactggtct
    45601 gagtaaagaa tgcgcgtata atgcccgccc ggtttgtgtt gttttgagag tttccttatg
    45661 gcgctgttaa tcactaaaaa atgcatcaat tgtgatatgt gtgaaatcaa ataaaaacac
    45721 atttattttc atagtgatac agcaatgtgt tattgaatgt gttgttggat ttatggtgca
    45781 aaaaaatttt gcataagttc agcaacacaa agaacaccac tttctttaac gtcctccgcg
    45841 ccctaaagga cggggcttta cggcgcaaaa ggcctgtgac atacaacatt aacttttcag
    45901 tcaaccgtag cacgttcttg tatacgccgt gtctgcggtt ttatataacc ctggctggaa
    45961 acctcttata caaagtcgat tcgccaacat catagatgat cgccaccttc tggcgagaaa
    46021 ctcctgatgc aattagtcgc ccggcctgcg cccattattc tggtgaaagt ttgggacggc
    46081 gaccgccaat tcgtccctgt gcgcgagcag cttccagtcc tgcttttgtt cgttcaacaa
    46141 tcagttcacg ttccatttca gccagggcac ccatcacatg gaagaaaaag cgtcccattg
    46201 gtgtgctggt atcaattgaa tcagtcagac tacgaaagtt gatgcctcgt tcgcgcaacg
    46261 cctccaccag cacgacaaga tgccgcatac tgcgccccag ccgatccagc ttccagacaa
    46321 ccagtgtgtc gcctgccgat aatgtcttga gcagtttttt cagccccggt ctttcggact
    46381 ttgtaccact tatcttgtct tcaaaaatca gctcgcatcc tgcacagttc aacgcattac
    46441 gttgtaga
//
LOCUS       DAZXUL010000019        45383 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZXUL010000019 DAZXUL010000000
VERSION     DAZXUL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45383
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.019"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..574
                     /locus_tag="ACH3L1_000988"
     CDS             <1..574
                     /locus_tag="ACH3L1_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4075831.1"
                     /translation="AEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLCM
                     DLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLDN
                     LKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKG
                     SDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="ACH3L1_000989"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="ACH3L1_000989"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFT4075832.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="ACH3L1_000990"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="ACH3L1_000990"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFT4075833.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="ACH3L1_000991"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="ACH3L1_000991"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFT4075834.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="ACH3L1_000992"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="ACH3L1_000992"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFT4075835.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="ACH3L1_000993"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="ACH3L1_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFT4075836.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="ACH3L1_000994"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="ACH3L1_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFT4075837.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="ACH3L1_000995"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="ACH3L1_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFT4075838.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="ACH3L1_000996"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="ACH3L1_000996"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFT4075839.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="ACH3L1_000997"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="ACH3L1_000997"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFT4075840.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="ACH3L1_000998"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="ACH3L1_000998"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFT4075841.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="ACH3L1_000999"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="ACH3L1_000999"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFT4075842.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="ACH3L1_001000"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="ACH3L1_001000"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFT4075843.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="ACH3L1_001001"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="ACH3L1_001001"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFT4075844.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="ACH3L1_001002"
     CDS             13934..14314
                     /locus_tag="ACH3L1_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFT4075845.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="ACH3L1_001003"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="ACH3L1_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFT4075846.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="ACH3L1_001004"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="ACH3L1_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFT4075847.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="ACH3L1_001005"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="ACH3L1_001005"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFT4075848.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="ACH3L1_001006"
     tRNA            complement(17415..17490)
                     /locus_tag="ACH3L1_001006"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="ACH3L1_001007"
     tRNA            complement(17494..17569)
                     /locus_tag="ACH3L1_001007"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="ACH3L1_001008"
     tRNA            complement(17621..17696)
                     /locus_tag="ACH3L1_001008"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
     gene            complement(17699..17774)
                     /locus_tag="ACH3L1_001009"
     tRNA            complement(17699..17774)
                     /locus_tag="ACH3L1_001009"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17739..17741),aa:Val,seq:tac)
     gene            complement(17910..17985)
                     /locus_tag="ACH3L1_001010"
     tRNA            complement(17910..17985)
                     /locus_tag="ACH3L1_001010"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17950..17952),aa:Lys,seq:ttt)
     gene            complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="ACH3L1_001011"
     CDS             complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="ACH3L1_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFT4075849.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18951..19472)
                     /gene="pal"
                     /locus_tag="ACH3L1_001012"
     CDS             complement(18951..19472)
                     /gene="pal"
                     /locus_tag="ACH3L1_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFT4075850.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="ACH3L1_001013"
     CDS             complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="ACH3L1_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFT4075851.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYP"
     gene            complement(20932..22173)
                     /gene="tolA"
                     /locus_tag="ACH3L1_001014"
     CDS             complement(20932..22173)
                     /gene="tolA"
                     /locus_tag="ACH3L1_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFT4075852.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22238..22666)
                     /gene="tolR"
                     /locus_tag="ACH3L1_001015"
     CDS             complement(22238..22666)
                     /gene="tolR"
                     /locus_tag="ACH3L1_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFT4075853.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22670..23362)
                     /gene="tolQ"
                     /locus_tag="ACH3L1_001016"
     CDS             complement(22670..23362)
                     /gene="tolQ"
                     /locus_tag="ACH3L1_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFT4075854.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23359..23763)
                     /gene="ybgC"
                     /locus_tag="ACH3L1_001017"
     CDS             complement(23359..23763)
                     /gene="ybgC"
                     /locus_tag="ACH3L1_001017"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFT4075855.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23913..24206)
                     /gene="ybgE"
                     /locus_tag="ACH3L1_001018"
     CDS             complement(23913..24206)
                     /gene="ybgE"
                     /locus_tag="ACH3L1_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFT4075856.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24206..24319)
                     /gene="cydX"
                     /locus_tag="ACH3L1_001019"
     CDS             complement(24206..24319)
                     /gene="cydX"
                     /locus_tag="ACH3L1_001019"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFT4075857.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24334..25473)
                     /gene="cydB"
                     /locus_tag="ACH3L1_001020"
     CDS             complement(24334..25473)
                     /gene="cydB"
                     /locus_tag="ACH3L1_001020"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFT4075858.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25489..27057)
                     /gene="cydA"
                     /locus_tag="ACH3L1_001021"
     CDS             complement(25489..27057)
                     /gene="cydA"
                     /locus_tag="ACH3L1_001021"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFT4075859.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27906..30539)
                     /gene="mngB"
                     /locus_tag="ACH3L1_001022"
     CDS             complement(27906..30539)
                     /gene="mngB"
                     /locus_tag="ACH3L1_001022"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFT4075860.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30557..32473)
                     /gene="mngA"
                     /locus_tag="ACH3L1_001023"
     CDS             complement(30557..32473)
                     /gene="mngA"
                     /locus_tag="ACH3L1_001023"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFT4075861.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32643..33365
                     /gene="mngR"
                     /locus_tag="ACH3L1_001024"
     CDS             32643..33365
                     /gene="mngR"
                     /locus_tag="ACH3L1_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFT4075862.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33468..34337)
                     /gene="sucD"
                     /locus_tag="ACH3L1_001025"
     CDS             complement(33468..34337)
                     /gene="sucD"
                     /locus_tag="ACH3L1_001025"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFT4075863.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34337..35503)
                     /gene="sucC"
                     /locus_tag="ACH3L1_001026"
     CDS             complement(34337..35503)
                     /gene="sucC"
                     /locus_tag="ACH3L1_001026"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFT4075864.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35597..36814)
                     /gene="odhB"
                     /locus_tag="ACH3L1_001027"
     CDS             complement(35597..36814)
                     /gene="odhB"
                     /locus_tag="ACH3L1_001027"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFT4075865.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36829..39630)
                     /gene="sucA"
                     /locus_tag="ACH3L1_001028"
     CDS             complement(36829..39630)
                     /gene="sucA"
                     /locus_tag="ACH3L1_001028"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFT4075866.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39931..40647)
                     /gene="sdhB"
                     /locus_tag="ACH3L1_001029"
     CDS             complement(39931..40647)
                     /gene="sdhB"
                     /locus_tag="ACH3L1_001029"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFT4075867.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLSGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40663..42429)
                     /gene="sdhA"
                     /locus_tag="ACH3L1_001030"
     CDS             complement(40663..42429)
                     /gene="sdhA"
                     /locus_tag="ACH3L1_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFT4075868.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42429..42776)
                     /gene="sdhD"
                     /locus_tag="ACH3L1_001031"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42429..42776)
                     /gene="sdhD"
                     /locus_tag="ACH3L1_001031"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFT4075869.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42770..43174)
                     /gene="sdhC"
                     /locus_tag="ACH3L1_001032"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42770..43174)
                     /gene="sdhC"
                     /locus_tag="ACH3L1_001032"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFT4075870.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43555..43662
                     /locus_tag="ACH3L1_001033"
     CDS             43555..43662
                     /locus_tag="ACH3L1_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075871.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43868..45151
                     /gene="gltA"
                     /locus_tag="ACH3L1_001034"
     CDS             43868..45151
                     /gene="gltA"
                     /locus_tag="ACH3L1_001034"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFT4075872.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT        11205 a        12103 c        11143 g        10932 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
    17761 tgagctaatc acccgacact acgctggata ctacaactaa ctatgttagt gggggtcgtg
    17821 caggatgact cgacttcgtc tcgcccttcg gcgccgttgc tgatgcaacg ttatccttca
    17881 cgttttacta ccgcttccac cttagcgatt ggtgggtcgt gcaggattcg aacctgcgac
    17941 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt
    18001 tcggtttgtt tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg
    18061 caacaaaaat ttcgatgcag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa
    18121 taatgcgact tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt
    18181 ttagcgccat cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt
    18241 gcggtgtcac ctttgtcctg catgatgacg ccgactttaa acatcgcatc agcagccttt
    18301 ggtgacttcg gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta
    18361 cccttgttgt agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca
    18421 gggtaatttt tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc
    18481 tgcaccagcg caatagctgc attgtaatcc gtgtttgcat cgccgctttt caccggcgcg
    18541 ccagcattcg cagtaccagc atcagctgtc ggcgtcgttg atgccgtcgc accgctttga
    18601 tcgctgctgg ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg
    18661 atctgctttt gccgctccac gacctgattc agttgatact gattttcctg aatctgacca
    18721 cgcagggaat caatatcgga ttgattatca gaaagttgtt gctggagttg ggttaaaagc
    18781 tggctgtgag cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag
    18841 ctgacactac tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt
    18901 aacgacagac tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc
    18961 agtaccgcac gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt
    19021 tctttaccgt aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac
    19081 atcttaacgg cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt
    19141 tcgtccgcgt gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca
    19201 tgtgcatcca gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa
    19261 acgatgttgt tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg
    19321 ttgccgttgc cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg
    19381 tcattgctgg cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc
    19441 atcagccctt tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa
    19501 ttattatcac ggatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag
    19561 acgcgctttg aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga
    19621 agagctgtag attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt
    19681 ggacgacaga acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc
    19741 accattggag ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt
    19801 ctgcgaacct tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac
    19861 ctgcggacga ccggcctggt cagaagtaaa tgccaggttc tggctatccg ggaaccaggt
    19921 cggttcggta ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc
    19981 cattacgtac aggttcagac taccggtttt cgacaaggcg aatgccagtt tgctgccgtc
    20041 tggcgagaat gcaggtgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc
    20101 atttgccagc gtctgaataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag
    20161 tttagaaccg tctggtgacc acgccggaga catcagcggc tgcggtgaac ggtgaacgac
    20221 aaactggttg taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt
    20281 ggtctgaaca acgtaggcaa tacgggtacg gaacgcacct ttaatgccgg tcagtttttc
    20341 aaacacttca tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta
    20401 cgagttctga gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac
    20461 attgtaagaa ccatccggat tcggagtgac ttgaccgaca actacagcgt caatgcccag
    20521 tgcggaccat gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc
    20581 gcgatctaac ggattaaatt taccgctgtt acgcaagtca gcagcaacga tgccgccaat
    20641 atcttcaggt gccgcaccag gccccgccca ctggaaagga acaacaccaa taggacgacc
    20701 ggaatctaca ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag
    20761 tatgagaaaa ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc
    20821 gatggcccac gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac
    20881 cccgtggcaa ccggtgcctg atgttgaccg tccgaacagt caacatcgcg attacggttt
    20941 gaagtccaat ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat
    21001 cttcgcaagt ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg
    21061 tttgatatcc agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc
    21121 tgcataggac gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata
    21181 gttattgata tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga
    21241 agcattgttc cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc
    21301 accgaaaata tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc
    21361 ttttgccgct gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc
    21421 tgctgcctta tcagcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc
    21481 tgccgctttc ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt
    21541 ggctttttca gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc
    21601 cgcttccgct ttcttcttca gtgccgcagc ggctacctcg gcttttttct gcgcttcggc
    21661 tgcggctttg gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc
    21721 tgcttcttcc gcagctttag catctgccgc cgctttcgct gccgccactt cagcttgctt
    21781 ctgctttaac tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc
    21841 taaccgctct ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag
    21901 ttcttcagca gcctgctgtt ccttcatctt gcgctgttca tcagaacgct tcgcgcttga
    21961 ttcctggctt tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc
    22021 gtcgatggac gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact
    22081 ccagatcagc gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt
    22141 gagcttgtcg ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg
    22201 ttacccgctc tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac
    22261 caaccgattt cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag
    22321 gcacatcttt tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg
    22381 acacttccgc caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca
    22441 cggtgtactg accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca
    22501 ccgcctgtga ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg
    22561 ctgtcgccat aaagatcagc aacagcacca gcagtacgtc cagcaacgga acaatgttga
    22621 tttcggactt gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt
    22681 tgctctcgct aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt
    22741 tgtcgtaatt cagttccagt ttgtttacgc gctggttgag acggttataa gccattaccg
    22801 ccggaattgc ggcgaacaga ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg
    22861 caaccatttg cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc
    22921 cccagaccgt accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg
    22981 gaatgtgcgt ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg
    23041 ccccttccac tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt
    23101 tgaacccgct gtaaaagatt tgttccgaac cagtcagatt atcccgtttc ccctgactct
    23161 cttgatagag gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt
    23221 cacgcgccgc tgcgttaaga atacgggttc gctggataat aatggcccag gatgcgattg
    23281 aaaaaccaat caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat
    23341 caaggatatt catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg
    23401 aggcttcatt ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt
    23461 attctcggcg ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat
    23521 ttcagtctgt atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat
    23581 tttacgtacc acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg
    23641 acgcagcatc tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac
    23701 accaccggca tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt
    23761 cactttacat cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg
    23821 cgcgattgag gtttgggaat gagggcaagt taagggcgcg taaaaattta tgggcctaga
    23881 gcggcccata attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca
    23941 atgagaacaa tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga
    24001 cgaaagccca cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc
    24061 cagatttcca gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct
    24121 gctaacagaa gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca
    24181 tacaaggtcg cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga
    24241 cgtgttcaag cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa
    24301 tccatgcgaa ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc
    24361 aatatcttct ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag
    24421 aatgatcggt accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga
    24481 agttgcatcc cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg
    24541 gaacattgcg ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa
    24601 cgcccacgcg gctttatcca tacgtgcagt caggatggtc agcagcagca gaaccacacc
    24661 cagtgccgga atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc
    24721 ttcacgaacc acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt
    24781 cacgacataa ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag
    24841 tgtcaccagc gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt
    24901 acgcatttgc agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc
    24961 cagcaggccg aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata
    25021 ttcatcaacg ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag
    25081 cggcggaacg aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc
    25141 ttcaatcttg gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag
    25201 gatcatcgcc acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa
    25261 gagtgcgccg cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga
    25321 gttaatcata attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac
    25381 ccccatgtcg aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca
    25441 ccagataaaa cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg
    25501 ctgagtagtc gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc
    25561 gaggcgtgca aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc
    25621 gcaaatcagc accattgaga agatgagatc gcctgcggtc agtgacgaat tcgccacagc
    25681 tgtcggcagc acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc
    25741 cgcttctaca gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt
    25801 tttctcgcca atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagcag
    25861 caggaagcca cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat
    25921 ggagtctttg gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata
    25981 gcgtttcagc agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac
    26041 cgtctggtcg gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt
    26101 acgaatgcgt tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac
    26161 ggaacgcgtt gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc
    26221 ctcttcctga tcaggaatgc cgaacaaagt aaaggcagca ggcgcaggtt gcgtttccca
    26281 ctcggcttca atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc
    26341 atcacccaga acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa
    26401 ggagcgttta gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag
    26461 gatgaacatc gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc
    26521 aaccgggtta agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt
    26581 gaaatcggac gcgattgggt tttgcatcca gccgttcgca accagaatcc acagtgcgga
    26641 caggtttgaa ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg
    26701 atcccaaccg aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc
    26761 ttcgattgcc agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt
    26821 agtcccgaac tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc
    26881 aaacaacttg ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta
    26941 gaccgtttcc ataatggcca ggaggaacgc cataccgagc gtcagtggca caaaaaggaa
    27001 gtggtacatc gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat
    27061 gactccttgc tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc
    27121 atgccccaat agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac
    27181 ggcgtaaaga aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt
    27241 acgccgcaaa atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg
    27301 gtgatcaatt tatagctaaa ttgccgcctt tcagccaatt tgatcgagaa caatttatct
    27361 ctttttgatg cccatttcca agacttatac attgataaat atcaatttct acaaattatg
    27421 ttaatcatgt ttcaacaagg taactttagt gatatttatg tttaaaatgt gtgaattata
    27481 agactgaaat tagtttcggt tggcaattaa agataaaaag agacgaagca aaaacagcac
    27541 ggttgacctt aattcttttt ttggtcactg gctaatacga cgcagagttt caggattatt
    27601 aataaaggca taaatctccc tcccgccgat attaacattt aattaactta taattatatt
    27661 ataattaact cacccgtggt tattattttg ttaaaaataa agccacatat acgtagcttg
    27721 agagtgaaga taaacacaaa attacctgat gcgctacgct tgccagtgtc tacatcatct
    27781 ctgcaatata ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg
    27841 gcatgaacaa agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt
    27901 gctattcagg caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat
    27961 agatttgaac cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa
    28021 gaaatcacct cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta
    28081 aacagccgta aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggcat
    28141 cccactgggg gcattttcaa caaactataa ctttccggca cgttgaatcc ggctttgttg
    28201 agcttcatcg catcccatgg gattttgttg taacactgta ctggagtcag ccatgctcgc
    28261 gcctgctgag ctacaccagc ggcggttggc gtaccggtat aactcaataa acttaggcga
    28321 caagaaagca gaccacgtag ttgtgagtcc gggactggca ttttaattcc cgaaggccgc
    28381 ctaggcctta aaagcagatc ttctttcccc agtaagccca cgccgcgcag caacgtaatg
    28441 gcaaaggttt tcttctcttc accgatgact tcaaattcac gtagcccttc gctaaagaca
    28501 gccatgccgt tacgcccttc ctgtaaggct gcatagttga gcatattcca taccggaacc
    28561 ggcgcttctt tccagctttc ttgctgccag ttgttcattg cactgtcgtt cacggggcgc
    28621 gttagcgaac caaactgggt atctgccaga acactatcgg tgttaaaaga tgtagggatc
    28681 aggacgcgaa ggcgatgatc gtcagcctgg ttatcaaggt tgatatccac atcaatgcgc
    28741 ctgttgttat gactaagagt gacaaccaac tcgacatcta ctctgccagt ggattgtctt
    28801 gcgctgcgtt gttgcagatt acgcggcacc gccatttcat agcggataac agccctgctc
    28861 tgccaggcct catgaataat ctcgcattgc ggtttcgcat tcgccgaagt aattacccac
    28921 tcttcttttg cgggtgaata gtcatattca tcaccatcat cagagctttc ttcaatttgc
    28981 aacacccggt catagcgcac accgctatct ttatctacca gttgtagcgt tccatcctca
    29041 ttgagcgcaa tttgccagaa agcattttcc agtatccctt cagcgtcact ttttgccgca
    29101 atcacgttgc caggctgatt cgcttcgata taaagcgtgc gatagcccat agaagggaca
    29161 atctggttga tctgtatatc aaactccata aagggatcgt aattaccgta atgaactatt
    29221 tgccgatcga ttaggcctgg atcgatctca cgggcatggc gaataaaata cggtacaggc
    29281 tgaccgcgat cgtctcgtag attaaactgg ctggcgcgca gccgcacagt ggtgttgatc
    29341 acttcttccc gcggccaggg catcaggtta aacaggacga gtttgtcggc gtcgctctgc
    29401 ggcatgttgt cggcgatttt acgcatatag aaacgcagca gattatccgc catgtcttca
    29461 gccagttcga agcgggcgac gatttcgcga tgaactttgt cacgacagca gcagccgatg
    29521 ctgtcgtggg catgattttt taagatctct ttccacattt tctccagcaa gccgtggtgg
    29581 tatccaaaac ccaacgtcct ggccagcgtt gccagcggtt ccagcagatt aacaatctta
    29641 ttttcaatac gtgcgtgggc aactttgata tccatgcgcg tggaaccgat ggtgcgatgc
    29701 acgcgcatat atttgccatc aataaattcc cctttcaggg ttgccagatt atctcgctgc
    29761 gcttcgatct tctcaaatac ctcttcaaag cggctcatca caaactttcg ttgaggataa
    29821 atttcgcgca gtttctccat cacttcgaag atattttgct gcaatggcat ctgatcatgc
    29881 ccattcggca gcaaaatctc tttggttaca gacgcttttt ccagcacgtc aaaataactg
    29941 tcgaggcgtt tgcgtaatcc gttttcgtcc gcaggtaagt acttaccgat ggcataaccc
    30001 aacggcatca cctgcgccgt cacttcgcta ccgtcactgc tttgccacaa aaactcggtt
    30061 ttatccgtac cgtggcgctc agaacatcca cgccagaaca tggtgcaggt aatgccaaat
    30121 ccattgtaga tatgcggcag ttgcccggac atgccaaagg aatcgggtaa ataacctatt
    30181 ttcatcggct cgccaaacgc gaggcagtca cgcattccgt acatcagatt acggacgatg
    30241 gattccgcag aaacaatcgt ggtatcggtc tgggtatacc aggggccgat aatcaacttg
    30301 ccggcttcta ccagtttctt caagcggtct ttgttttccg gtttcaccgc gaaataatct
    30361 tcgaggatcg ccgtttgccc gtcgagtacg taatatttgt attcgttgtc ctgttccagt
    30421 cggcacagga tctcttccat attattgacc agcagaatgc gtgactcttc ggtggtgaaa
    30481 taccactccc gatcccagtg catatgcggg gtgatgtgaa cgcgagatac cgctttcatc
    30541 gtcgtttcct ttttgtttat ggcattacgc catcagtcag atagttgcca tgcttaaccg
    30601 cgtgacgccg ccagatcagg agaattgcag tcgagattgc agcccccacc aatgccgcac
    30661 caaaccagcc aattgccgcc ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg
    30721 agaaaatgcc agcaccgggt gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg
    30781 ttaccataga gcccagcaca aacgaaccaa taacccgcag cggatcttca atcgccatcg
    30841 ggatcgcccc ttcggtaata cctgccagcc ctaacagcca ggtggatttc ccggtctcaa
    30901 tttcaaactc tttaaacagg cgcggtgcga gcatcgtgga agcggttacg gtaaatgccg
    30961 aaaccatttt gacggaggcg aaaatggcat acgggccgta aacgccgttc gccattgcgc
    31021 ccaggcagaa tgcataagcg gctttattca ctggccctcc aaggtcaaag gaacacataa
    31081 aaccgagaat ggcacccagc aacagcgcgt tacttcctga cagaccgttc agccaggcgg
    31141 taagcgagtt attgatccag gcgacaggtt cccccaccac aaacagcatc agactgcccg
    31201 ctcccaatgt accgagcacc gggtagagat aaaaagtcag gaagccattg aatttactgc
    31261 taagacgcaa gtgatttttc atccagcgta tcaagtaacc ggctatcaat ccgccaacga
    31321 ccgcgccgag aaacccggag ccgatcatgt tggcggcaag tccagccgca aagcctggcg
    31381 ctaacgccgg tttatctgcc agcgaatagg cggtataggc tgccagcacc ggtaccatca
    31441 aaattccgag cagcccgccg cccagtttgc ggtacatcca cagccaggag ttttcttcat
    31501 taaacagatc ttgtagcccg aagatttgcg acagtaatac cgcgaccgcc agcaccgtcc
    31561 cccccgcgac aatcaacggt acggcaaaag agatcccgct caacagtgcc tgtttcagct
    31621 ccgttttgac gcttttcacc ggttgcgtat cttgctgtaa ggtacgcgtc tcatcgccac
    31681 gctcgagggt aagcgattgt tggatcaacg cttctgcatg gcgaatcggc tcggcaacag
    31741 gcacagaaag cgcaggaatg ccgttaaaac gctcactctc cttgatggcg acttcagccg
    31801 caaaaataca ggcagttgca ctattgagtt gatccgccgt taaacgccct tcaataccgt
    31861 tagcaccttg cttttcaacg tagacattca cgccgagttt gcgtccggct ttttccagat
    31921 actccgcagc catgtaggtg tgagcaatac ccgccgggca ggccgttacg caaacgatag
    31981 ttggcgcgtt ggaaaaagag gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga
    32041 gcaattcatc aggcgtcgtc gccgactgta tacgcgcccg aatctcatca tccgccaggc
    32101 gcgtggtcag cgctgtcagc agttgcatgt gcgtagtacc cgcttcattg aggggaatcg
    32161 ccagcaacac cactaaatca accgcttccg ggccatccac gccttcccac tgaagcggct
    32221 cgctgagtgt ggcgaccgca aacgccgctt cttttaccgc agcagttttg ccatgcggca
    32281 cagccaaccc ttcacttaag gcggtcgggc caaggctttc acggcgatac acttctttca
    32341 gaaattgctc agtactggaa attttcccca gagcagcaag acgttgagtc aacgcgtgaa
    32401 tggcctcttc acggctggta aagcgcgcat tcagacacaa cgcatcgcgg tgggtcagag
    32461 tcgtcaggtt catcatgatc ctcacttgat cgtttttata gtcgcgccag tgtgcccgac
    32521 aaaacaatac cattgcgtaa tcaatttcac ttgattgaaa ttatttgcat tttatttgta
    32581 ttgtttacag gatgagttta tcgggcataa tggctaaatt cgtattaatg agatgctaaa
    32641 taatgggaca caaaccctta taccgacaga ttgctgatcg cattcgtgag caaattgcgc
    32701 gtggcgagct taaacctggc gatgcgttac ctaccgaatc ggcattacaa acagagtttg
    32761 gcgtcagccg ggttacggtg cgtcaggctt tgcgccagtt agtcgagcaa cagatcctcg
    32821 aaagcattca gggcagcggg acttacgtca aagaagagcg ggtcaattac gatatttttc
    32881 agttaaccag ttttgatgaa aaactgtcgg accgtcacgt cgatacacac agtgaagttc
    32941 tgatatttga agtgattccg gctgacgatt ttcttcagca acagctacaa atcaccgtgc
    33001 aggatcgtgt ttggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg
    33061 aagaaacctg gatgccgctt gcgttgttcc cggatctcac ctggcaggtc atggaaaatt
    33121 cgaaatatca ctttattgaa gaagtgaaga agatggttat cgatcgtagc gaacaggaaa
    33181 tcattcctct gatgccaacc gaagagatga gccgtctgct taatatcagc cagacaaagc
    33241 cgattcttga aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt gaatatagcc
    33301 gcaacgcttt taataccgat gactataaat ttaccctgat agcgcagcga aaatcatcgc
    33361 gataagcaca aaaaggccat catacgatgg ccaaccatgt cgaaaccgac agcccttcgc
    33421 cattcctgta gagggaagcg gcgagggcta tttcttatta cagatattta tttcagaaca
    33481 gttttcagtg cttcaccgat atccgccagg ctgcgaacgg ttttcacgcc tgcggcttcc
    33541 agagcagcga atttctcatc cgcagtccct ttcccaccgg caatgatcgc acccgcgtgg
    33601 cccatacgtt tgcctttcgg cgcagtcaca ccagcgatgt aacccacaac tggcttggta
    33661 acatgctctt tgatgtacgc tgccgcttct tcttcagcgc taccgccgat ctcaccgatc
    33721 atcacgatcg cttcggtctg cggatctttt tcgaacattt cgagaatgtc gataaagtta
    33781 gagccaggga tcgggtcacc gccgataccg acacaggtcg actgaccgaa accgtaatcc
    33841 gtggtctgtt taaccgcttc ataggtcagt gtaccggaac gggaaacgat acccacttta
    33901 cccggtttgt gaatgtgacc cggctggata ccaattttgc attcacccgg ggtgataacg
    33961 cctgggcagt tcgggccgat catacgaacg cctgcttcgt ccagtttcac tttcacggtc
    34021 agcatatcca gcgtcgggat gccttcagtg atggtgataa tcagtttgat gcctgcatcg
    34081 atagcttcca gaatggagtc tttgcagaac ggtgctggta cgtaaataac agacgcggaa
    34141 gcgccagtag cagcaacagc ttcacgcacg gtgttgaaca ccggcaggcc gaggtgggta
    34201 gtgccgcctt tacctggagt tacgccacca accattttag tgccgtatgc aatggcctgt
    34261 tctgagtgga aagtcccctg gctaccggta aagccctggc agataacctt ggtgttttta
    34321 tcaattaaaa tggacattat ttcccctcca ctgcggcaac aacctgctga gctgcatccg
    34381 tcagaccttt tgctgcaata atattcaggc cgctgtcagc cagtttcttc gcgccgagtt
    34441 cggcgttgtt accttccaga cgtaccacga ccggtacgtt aacacccact tccgctaccg
    34501 cgccgatgat accgtcagcg atcaggtcgc aacgaacgat accgccgaag atgttaacca
    34561 gaacggcttt cactttgtcg tcagagagga tgattttgaa cgcttcggtt acacgttctt
    34621 tggttgcgcc gccgccaacg tccaggaagt tagccggttc gccgccgtgc agtttaacta
    34681 tgtccatcgt acccatcgcc agacctgcgc cgttaaccat acaaccgatg ttaccgtcca
    34741 gcgcaacgta gttcagttcc cactgtgcag cctgtgcttc acgcggatct tcctgcgact
    34801 ggtcacgcat ttcgcgcaga tcaggctggc ggaacagggc gttgccatca gcgcccagtt
    34861 tgccgtcgag gcaaatcaga tcgccctgtt tggtgatgac cagcgggttg atttcgatca
    34921 gcgccaggtc gcgctccagg aaaatggtcg ccaggcccat gaagattttg gtgaactgct
    34981 gaaccagttt accttccaga cccagtttga acgccagctc gcgtccctga tacggcatcg
    35041 ggccagtcag cggatcaagc gcaactttat ggatcaggtg cggagtttct tccgccactt
    35101 tttcgatttc cacgccgcct tcggtggagg ccataaagac cacgcgacgg gaactacggt
    35161 ctacaacggc accgagatac agctctttag cgatatcggt cgccgcttca accagaatct
    35221 ggttaaccgg ttggccattg gcatctgttt gatacgttac cagacgcttg cccagccagt
    35281 tttctgcaaa agcacggatg tcttctttgc tgtttacaac tttcacaccg cccgctttac
    35341 cgcggccacc agcgtgaacc tgacatttca ctacccacgg accggcaccg atttttgaag
    35401 cggcttcttc tgcttcgcgc ggagtagtac aggcataacc caccggtgct ggtaagccat
    35461 agcgggcaaa cagttgtttt gcctgatatt catgtaagtt catgtgttct gtccatcctt
    35521 cagtaatcgt tatcttttaa accgtagacc gacaccctat ccacgtcggt ctacagtgca
    35581 ggtgaagctt aaactactac acgtccagca gcagacgcgt cggctcttcc agtaactctt
    35641 tgatcgttac caggaagccc acggattcgc gaccatcgat cagacggtga tcgtaggaca
    35701 gcgccaggta catcatcggc aggatctcaa cctgaccatt caccgccatc ggacgatctt
    35761 tgatagcgtg catacccaga attgcgctct gcggcgggtt gatgatcggc gtagacatca
    35821 gggaaccgaa cacaccaccg ttggtgatgg tgaagttacc accggtcaga tcttcaaccg
    35881 tcagcttgcc atcacggcct ttgactgcca gctctttgat tttcttctcg atgtctgcca
    35941 tgccgagggt atcgacatca cgcagaaccg gcgtcaccag gccgcgcggc gtagaaaccg
    36001 ccatgctgac gtcgaaatag ttgtggtaaa ccacgtcatc gccgtcgata gacgcgttca
    36061 cttccgggta acgtttcagg gcttcaacca ccgctttcac gtagaaggac ataaagccca
    36121 gacggatgcc gtggcgtttt tcaaacgctt caccgtactg cttacgcaga tccataatcg
    36181 gcttcatgtt gacttcgttg aacgtggtca gcatggcggt ggagtttttc gcttccagca
    36241 gacgctctgc cacacgctta cgcaggcgag tcatcgggac acgtttttca ctacgtgcag
    36301 ccagagccgg ttgcgccgcc ggagcagccg ctgccggagc agactctttc gccggggctt
    36361 tcgccagatg tttttccaca tcttcacgag tcagacgacc acccacaccg gtgcctttaa
    36421 tggcgctggc gtcgagattg tgttcagcca gcaggcgacg gatcgccggg cttaacgcat
    36481 cgttgttttg ctcttccaga gacgcctgct ggcgttgcgc cggagtggac gctttctctt
    36541 cagatttggc gctggtttct ttaccggcgc tgttgccttc acgcaggcga ccaaggatct
    36601 gacgagacgt taccgttgta ccttcatctt ccagaaccgc atccagaatg ccgtctgctg
    36661 atgccggtac ttccagtacc actttgtcag tttcgatttc taccagcact tcatcacgta
    36721 cgactgcgtc gccgggtttt ttatgccagg ttgcgacggt ggcatcggct acggattcag
    36781 gcaggtcagg gaccagaata tctacgctac tcattgtgta tcctttattt attcgacgtt
    36841 cagcgcgtca ttaaccagat cttgttgctg tttctggtga acggacatat accctaccgc
    36901 cggagaggcg gaggccgggc ggcctgcata acgcagagaa gccccaaacg gaatcacttc
    36961 acggaaatga tgctggctgc agtaccatgc gccctggttg agcggctctt cctggcacca
    37021 gacaaaatcc ttgacgtgag caaactgctg caacacttcc tgcatcgctt tatgcgggaa
    37081 cgggtagagt tgctcgatac gcacaatggc gacatcgtgt tgattgttct tacgacgctg
    37141 ttccagcagg tcgtaataaa ccttaccaga acacatcact acgcgcttca cgcccttcgg
    37201 atcaagctcg tcgatttcac cgatggctgg caggaaggtg ccgttcgcca gttcttcgag
    37261 gctggaaacc gccagcggat gacgcagcag ggatttcggc gacatcacga ccagcggacg
    37321 acgcatcccg cgcagcgcct gacgacgcag catgtggtaa acctgtgccg gggtagacgg
    37381 tacgcaaacc tgcatgtttt gctcagcaca aagttgcaga taacgttcca gacgcgcgga
    37441 ggagtgctcc ggcccctgcc cttcgtaacc gtgcggcagc aacatcacca gaccacacat
    37501 ccggccccat ttctgctcgc cagaggagat gaactggtcg ataaccacct gcgcaccgtt
    37561 ggcgaagtca ccgaactgcg cttcccagat ggtcagggtg cgtggttctg cggtggcata
    37621 accatattca aacgccagca cggcttcttc tgacagtacg gagtcccaga cacggaacgc
    37681 gccctgaccg ttatggatgt gttgcagcgg cgtgtaagtg gaaccgttag actggttgtg
    37741 gatcaccgcg tggcggtgga agaaggtacc gcgaccggag tcttcacccg acaggcgaac
    37801 cggaatgcct tcatcaacca gcgtggcgta agcgaggttt tccgcaccgc cccagtcgaa
    37861 cagtttctca ccggctgcca tcgcctggcg atcgccataa atcttggcaa cgcgagactg
    37921 catttcaact gcttctggta ccgtactgat acgtttagcc agttcctgca ggcgcttcat
    37981 ctcaactttg ttcgggtact cttcgtccca ttcgtggttg aggtacggcg accaggtgaa
    38041 agagtgcatg ttcatcggac gccactctgc cactacgcaa tcgccagcat ccagcgcatc
    38101 gcggtacagg ttaaccatct cggtggcatc ttccagcgtc gccacttttt cctgctccag
    38161 cttgtcagcg tagattttgc gcggtgtcgg atgttttttg attttctgat acatcagtgg
    38221 ctgggttgcg ctcggctcgt cggcttcgtt gtggccgtga cggcggtagc ataccaggtc
    38281 gatgaagaca tcacgtttaa aggtgttacg gaaatcgagc gccagacggg tcacaaaggc
    38341 aacggcttcc ggatcatccg cgttaacgtg gaaaatcggt gcctgaacca tcttaccgat
    38401 atcagtacag tacggtgtag aacgggcatc cagcgggtta gaggtggtga aaccaacctg
    38461 gttgttgata acgatacgta ccgtaccgcc aacttcataa ccacgcgctt tcgacatgtt
    38521 cagagtttcc tgaaccacgc cctgcccggt cactgcggcg tcaccgtgga tggtgattgg
    38581 cagcactttg ttgctgttcg gctcatcaag tctgtccaga cgggcacgaa cagagccgat
    38641 aacgaccggg cttacaatct caaggtgaga cgggttaaac gccagcgcca ggtgcaccag
    38701 gccgccatcg gtctggaagt cagacgagaa gcccatgtgg tatttcacgt cgcctgtgcc
    38761 gaggtgttct ttatgtttac cggcgaactc gtcgaacaag tcttgcggtt ttttacccag
    38821 cacgttcacc agcacgttca gacgaccacg gtgcgccatc ccgagaacca cttcgcgggt
    38881 gccgctgttg ccagcgtggc ggatcatctc tttaagcatc gggattaacg cgtcaccgcc
    38941 ttccagcgag aagcgttttg cgccagggaa ttttgcgccg aggtaacgct caaggccttc
    39001 agcggcggtc agttcgctta agaagcgttt tttctcttcg ctattgaaag tcgcgcgacc
    39061 agactcgata cgctgttgga tccagcgttt ttcttcggtg ctggtgatgt gcatatactc
    39121 ggcaccaatc gggccgcagt aggtttgctt gagggcggaa ataagctcgc cgagtttcat
    39181 ggtttctttg ccgctggcaa atgaaccgac gttgaaggtt tcctggaagt ctgcttcggt
    39241 cagatcgtgg aaagacggat ccagatcggc cactttatct tgctgccaca gtcccagcgg
    39301 atctagattc gcatgctggt gaccacggaa gcggtatgcg ttaatgagct gcaggacttt
    39361 aacctgcttc acattggtgt cagggtcgga gatcgttgaa gagtaacgtg aagcgtcttt
    39421 cgccaggcgg cggaaatatt cacgcgtttg agagtggaat tgatccggtt tgactcccgt
    39481 accaggtaac tgctggaacg tcgaacgcca gttagcgtca accgagtcag gatcggttaa
    39541 gaagtcttca tagagctgtt ctatccagct ctggtttgcg ccagagaggt aagaagagtc
    39601 caaccaggct ttcaaagcgc tgttctgcat cgtgatccct taagcatctt ttttatgctt
    39661 acttcgccgt ggatactacc acgcacagtg atgtgcgtgc cggggttcac ttgcgagctc
    39721 ttgtctacgt agtggctcgc gaaggaacct ttaaaaactg tcttagtgtc aggggcagtt
    39781 tttaaagatt tcctgcattt ctttgttacg cctgatgcgc tgcgcttatc aggcctgcgg
    39841 gtaatgactt gtaggccgga taaggcgttc acgccgcatc cggcactggt tgcctgatgc
    39901 aacgcttgcg cgtcttatca ggcctacagt ttacgcatta cgttgcaaca acatcgactt
    39961 gatatggccg atggcgcgcg tcgggttcag ccccttcgga catacactga cgcagttcat
    40021 gatgctgtga cagcggaata cgctgaatgc atcgctcaaa ccgtcgaggc ggctgtcagt
    40081 ctcggtatca cggctatcga tcaggaaacg atatgccgct aacaagcctg ccgggccgat
    40141 aaacttatcg ggattccacc agaaagacgg acaagaggtt gaacaacatg cgcagagaat
    40201 acattcatac agcccgtcga gtttttcgcg ctgctctggc atctgtaaat gctcgcgagc
    40261 tggcggattt tgtccattat tcaacaggta aggcttaatt ttctcatatt gcgcatagaa
    40321 ttgtcccatg tctaccacca aatcgcggat caccggtaaa cctgacagcg ggcgaatcac
    40381 aatcttcttg cccggctggt tgagtgccga aatcggggta atacaggcca gaccattctt
    40441 gccgttcatg ttcagaccgt cggaaccgca cacaccttca cggcaggagc ggcggaacga
    40501 caggctggga tctttctctt tcagctggat aagcgcatcc agcagcatca tgtcgcgacc
    40561 ttcttccgct tccagggtgt aatcctgcat acgcggagca tcatcaacat ccgggttata
    40621 gcgataaatt gaaaactcga gtctcatttt cctgtctccg cattagtaag tacgaatctt
    40681 cggcgggaat gccgggcgca gtttcggttc catgttgacg cttcggcgcg tcatggattc
    40741 cgactcaggc agatacaggg agtggcacag ccagttttca tcatcacgat ccgggaagtc
    40801 gaagcggcta tgtgcgccac ggctttcggt acggaagttg gcagaaacag ccgttgcata
    40861 cgccgtttcc atcaggttat ccagttccag gcactcaacg cgctgggtgt tgaactcgct
    40921 ggaagtgtca tccagacggg catttttcag acgctcgcgg atcactttca actgctcaag
    40981 ccctttcgcc atcgcatcac cttcacggaa gaccgagaag ttatgctgca tacattcttg
    41041 cagcgcttta cggatcgcca ccggatcttc accgttacga ttattgttcc accggttcag
    41101 gcgatccaga gacgcttcaa catcagactc gctggcatcg tgcagtgcgc cctgctcggc
    41161 gatagactct tgcagatgca gacctgccgc gcgaccaaag accaccaggt ccagcagcga
    41221 gttgccgccc agacggttag cgccgtgtac cgatacacaa gcgatttcac caacggcaaa
    41281 cagtcccgga acaaccacat cttcgccttt ctcattcaca gtcagcgcct gaccggtaac
    41341 tttggtcgga ataccgccca tcatgtagtg acaggttggg ataaccggaa tcggctcttt
    41401 caccggatcg acgtgagcga aggtacggga aagctccagg atacccggca gacgggattc
    41461 gagaacttct ttacccaggt gatcgagttt cagtttcgcg tgtggccccc acggaccatc
    41521 acagccgcga ccttcacgga tttcaatcat gatggaacgc gccaccacat cacggcccgc
    41581 caggtctttg gcgttcggcg cataacgctc cataaagcgt tcgccatgtt tgttcagcag
    41641 ataaccgcct tcaccacggc aaccttcggt gaccagtacg cccgcaccgg caatgccggt
    41701 cgggtggaat tgccacattt ccatatcctg caccggtacg cctgcgcgga tagccatgcc
    41761 gacaccgtcg ccagtgttaa tgtgggcgtt agtggtggac tgataaatac gccctgctcc
    41821 gccagtcgcc agcacggtag cgcgggcttt gaaataaacc acttcaccgg tttcgatgca
    41881 cagtgcggta caacccacca ccgcgccatc ctggtttttc accagatcca gcgcatacca
    41941 ctcggagaaa atggtggtgt ggtttttcag gttctgctga taaagcgtgt gcaacagtgc
    42001 gtgaccggta cggtcagcag ccgccgcagt gcgtgccgcc tgctcgccgc cgaagttttt
    42061 cgactgaccg ccaaacggac gttgatagat acgaccatca tcgagacgcg agaacggcag
    42121 gcccatgtgt tcgagttcca gaatcgcttc cggcccggtt ttacacatat attcaatcgc
    42181 gtcctggtca ccgatatagt ccgacccttt tacggtgtcg tacatatgcc attcccagtt
    42241 atcttcatgg gtattaccca gcgcaacggt aatgccgcct tgcgcagaaa cggtatggga
    42301 acgggtcggg aagactttag agagcagggc acaggtctgg ccgctctggg aaatttgcag
    42361 cgccgcgcgc atacctgcgc caccggcacc aatcacaact gcatcaaatt ctctgactgg
    42421 caatttcatc acacacccca caccacaacg aatccataaa tcacgtaaac caccagtgca
    42481 acgacaatca ccagttgcag catcaggcgc aaggccagcg gtttaacgta gtcggtcaac
    42541 acctgccaca tgccgatcca ggcatgaatc aagatagaaa acagcgccag cagggtgaac
    42601 actttggtga acgcagaggc gaagaaaccg atccagactt catatgtcag ttcgccactg
    42661 gtggcgaaaa aaccgaccat ataaatgatg tagagcgtca ggacgatagc ggtagcgcga
    42721 acgaggatga aatcatgtac gccattgcgt cctaatgcgg aggcgttgct taccatacga
    42781 ggactcctgc gagaagtgaa agcacgacag taataacaaa ggagattttg gcggagcgtt
    42841 tacccgcttc gaatgtttct tccagatagc caaaatccat catcatgtgg cgaataccta
    42901 cgacgacgtg atacgccaga gcggtaagga tgccccacat gataaatttg acgaagaagc
    42961 tgcccataat cgcggaagct tgctcgaaac cttcagggga agagaggctg gtacccagaa
    43021 gccactgcag gatgcccact gcaacaaagg tgatcacacc ggaaacgcga tggagaatgg
    43081 acgctatcgc cgtgatgggg aaccggatgg tctgtaggtc cagattaaca ggtctttgtt
    43141 ttttcacatt tcttatcatg aataacgccc acatgctgtt cttattattc cctggggact
    43201 acgggcacag aggttaactt tctgttacct ggagacgtcg ggatttcctt cctccggtct
    43261 gcttgcgggt cagacagcgt cctttccata actgcgcgtc atgcaaaaca ctgcttccag
    43321 atgcgaaaac gacacgttac aacgctgggt ggctcgggat tgcagggtgt tccggagacc
    43381 tggcggcagt ataggctgtt cacaaaatca ttacaattaa cctacatata gtttgtcggg
    43441 ttttatcctg aacagtgatc caggtcacga taacaacatt tatttaattt ttaatcatct
    43501 aatttgacaa tcattcaaca aagttgttac aaacattacc aggaaaagca tataatgcgt
    43561 aaaagttatg aagtcggtat ttcacctaag attaacttat gtaacagtgt ggaagtattg
    43621 accaattcat tcgggacagt tattagtggt agacaagttt aataattcgg attgctaagt
    43681 acttgattcg ccatttattc gtcatcaatg gattctttac ctgcaagcgc ccagagctct
    43741 gtacccaggt tttcccctct ttcacagagc ggcgagccaa ataacaaacg ggtaaagcca
    43801 ggttgatgtg cgaaggcaaa tttaagttcc ggcagtctta cgcaataagg cgctaaggag
    43861 accttaaatg gctgatacaa aagcaaaact caacctcaac ggggacacag ctgttgaact
    43921 ggatgtgctg aaaggcacgc tgggtcaaga tgttattgat atccgtactc tcggttcaaa
    43981 aggtgtgttc acctttgacc caggcttcac ttcaaccgca tcctgcgaat ctaaaattac
    44041 ttttattgat ggtgatgaag gtattttgct gcaccgcggt ttcccgatcg atcagctggc
    44101 gaccgattct aactacctag aagtttgtta catcctgctg aatggtgaaa aaccgactca
    44161 ggaacagtat gacgaattta aaactacggt gacccgtcat accatgatcc acgagcagat
    44221 tacccgtctg ttccacgctt tccgtcgcga ctcacatcca atggcagtca tgtgtggtat
    44281 taccggcgcg ctggcggcgt tctatcacga ctcgctggat gttaacaatc ctcgtcatcg
    44341 tgaaattgcc gcgttccgcc tgctgtcgaa aatgccgacc atggccgcga tgtgttacaa
    44401 gtattccatt ggtcagccat ttgtttatcc gcgcaacgat ctctcctatg ccggtaactt
    44461 cctgaatatg atgttctcca cgccgtgcga accgtatgaa gttaatccga ttctggaacg
    44521 tgctatggac cgtattctga tcctgcacgc tgaccatgaa cagaacgcct ctacctccac
    44581 cgtgcgtacc gctggctctt cgggggcgaa cccgtttgcc tgtatcgcag caggtattgc
    44641 ttcactgtgg ggacctgcgc acggtggtgc taacgaagcg gcgctgaaaa tgctggaaga
    44701 aattagctcc gttaaacaca ttccggaatt tgttcgtcgt gcgaaagata aaaatgattc
    44761 tttccgcctg atgggcttcg gtcaccgcgt gtacaaaaat tacgacccgc gcgccaccgt
    44821 aatgcgtgaa acctgccatg aagttctgaa agagctgggc accaaagatg acctgctgga
    44881 agtggctatg gagctggaaa acatcgcgct gaacgacccg tactttatcg agaagaaact
    44941 gtacccgaac gtcgatttct actctggtat catcctgaaa gcgatgggta ttccgtcttc
    45001 catgttcacc gtcattttcg caatggcacg taccgttggc tggatcgccc actggagcga
    45061 aatgcacagt tacggtatga agattgcccg tccgcgtcag ctgtatacag gatatgaaaa
    45121 acgcgacttt aaaagcgata tcaagcgtta atggttgatt gctaagttgt aaatatttta
    45181 acccgccgtt caaatggcgg gttaattttt atatgcctaa acacaaaaaa ttgtaaaaat
    45241 aaaatccact atcggacatt catatattct aaaaaaaaat acatcggtat aaattataaa
    45301 caaatagatg atttttaatc atctatcttc ctctcagtta catgaataaa ccaccaaata
    45361 tagcgcaccc ctataattcc caa
//
LOCUS       DAZXUL010000020        45091 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZXUL010000020 DAZXUL010000000
VERSION     DAZXUL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45091
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.020"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(131..1795)
                     /gene="glnS"
                     /locus_tag="ACH3L1_001035"
     CDS             complement(131..1795)
                     /gene="glnS"
                     /locus_tag="ACH3L1_001035"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFT4075873.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1998..3944)
                     /gene="nagE"
                     /locus_tag="ACH3L1_001036"
     CDS             complement(1998..3944)
                     /gene="nagE"
                     /locus_tag="ACH3L1_001036"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFT4075874.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4277..5077
                     /gene="nagB"
                     /locus_tag="ACH3L1_001037"
     CDS             4277..5077
                     /gene="nagB"
                     /locus_tag="ACH3L1_001037"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFT4075875.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5137..6285
                     /gene="nagA"
                     /locus_tag="ACH3L1_001038"
     CDS             5137..6285
                     /gene="nagA"
                     /locus_tag="ACH3L1_001038"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFT4075876.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6294..7514
                     /gene="nagC"
                     /locus_tag="ACH3L1_001039"
                     /gene_synonym="nagR"
     CDS             6294..7514
                     /gene="nagC"
                     /locus_tag="ACH3L1_001039"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFT4075877.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7562..8314
                     /gene="nagD"
                     /locus_tag="ACH3L1_001040"
                     /pseudo
     CDS             7562..8314
                     /gene="nagD"
                     /locus_tag="ACH3L1_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            complement(8365..8463)
                     /locus_tag="ACH3L1_001041"
                     /pseudo
     CDS             complement(8365..8463)
                     /locus_tag="ACH3L1_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8711..10375
                     /gene="asnB"
                     /locus_tag="ACH3L1_001042"
     CDS             8711..10375
                     /gene="asnB"
                     /locus_tag="ACH3L1_001042"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFT4075878.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10754..10830
                     /locus_tag="ACH3L1_001043"
     tRNA            10754..10830
                     /locus_tag="ACH3L1_001043"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10788..10790,aa:Met,seq:cat)
     gene            10839..10923
                     /locus_tag="ACH3L1_001044"
     tRNA            10839..10923
                     /locus_tag="ACH3L1_001044"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10873..10875,aa:Leu,seq:tag)
     gene            10947..11021
                     /locus_tag="ACH3L1_001045"
     tRNA            10947..11021
                     /locus_tag="ACH3L1_001045"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10979..10981,aa:Gln,seq:ttg)
     gene            11056..11130
                     /locus_tag="ACH3L1_001046"
     tRNA            11056..11130
                     /locus_tag="ACH3L1_001046"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11088..11090,aa:Gln,seq:ttg)
     gene            11146..11222
                     /locus_tag="ACH3L1_001047"
     tRNA            11146..11222
                     /locus_tag="ACH3L1_001047"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11180..11182,aa:Met,seq:cat)
     gene            11271..11345
                     /locus_tag="ACH3L1_001048"
     tRNA            11271..11345
                     /locus_tag="ACH3L1_001048"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11303..11305,aa:Gln,seq:ctg)
     gene            complement(11499..12674)
                     /gene="ubiF"
                     /locus_tag="ACH3L1_001049"
     CDS             complement(11499..12674)
                     /gene="ubiF"
                     /locus_tag="ACH3L1_001049"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFT4075879.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12820..14244
                     /gene="miaB"
                     /locus_tag="ACH3L1_001050"
     CDS             12820..14244
                     /gene="miaB"
                     /locus_tag="ACH3L1_001050"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFT4075880.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14397..15437
                     /locus_tag="ACH3L1_001051"
     CDS             14397..15437
                     /locus_tag="ACH3L1_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFT4075881.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15434..15901
                     /gene="ybeY"
                     /locus_tag="ACH3L1_001052"
     CDS             15434..15901
                     /gene="ybeY"
                     /locus_tag="ACH3L1_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFT4075882.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15991..16869
                     /gene="corC"
                     /locus_tag="ACH3L1_001053"
     CDS             15991..16869
                     /gene="corC"
                     /locus_tag="ACH3L1_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFT4075883.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16894..18432
                     /gene="lnt"
                     /locus_tag="ACH3L1_001054"
     CDS             16894..18432
                     /gene="lnt"
                     /locus_tag="ACH3L1_001054"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFT4075884.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18829..19737
                     /gene="gltI"
                     /locus_tag="ACH3L1_001055"
     CDS             18829..19737
                     /gene="gltI"
                     /locus_tag="ACH3L1_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFT4075885.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19907..20647
                     /gene="gltJ"
                     /locus_tag="ACH3L1_001056"
     CDS             19907..20647
                     /gene="gltJ"
                     /locus_tag="ACH3L1_001056"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFT4075886.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20647..21321
                     /gene="gltK"
                     /locus_tag="ACH3L1_001057"
     CDS             20647..21321
                     /gene="gltK"
                     /locus_tag="ACH3L1_001057"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFT4075887.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21321..22046
                     /gene="gltL"
                     /locus_tag="ACH3L1_001058"
     CDS             21321..22046
                     /gene="gltL"
                     /locus_tag="ACH3L1_001058"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFT4075888.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22164..23099
                     /gene="rihA"
                     /locus_tag="ACH3L1_001059"
     CDS             22164..23099
                     /gene="rihA"
                     /locus_tag="ACH3L1_001059"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFT4075889.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            23183..24853
                     /gene="hscC"
                     /locus_tag="ACH3L1_001060"
                     /pseudo
     CDS             23183..24853
                     /gene="hscC"
                     /locus_tag="ACH3L1_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(24913..26364)
                     /gene="djlC"
                     /locus_tag="ACH3L1_001061"
     CDS             complement(24913..26364)
                     /gene="djlC"
                     /locus_tag="ACH3L1_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFT4075890.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(26361..27068)
                     /locus_tag="ACH3L1_001062"
     CDS             complement(26361..27068)
                     /locus_tag="ACH3L1_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFT4075891.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            27170..27724
                     /locus_tag="ACH3L1_001063"
     CDS             27170..27724
                     /locus_tag="ACH3L1_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFT4075892.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(27734..29161)
                     /locus_tag="ACH3L1_001064"
     CDS             complement(27734..29161)
                     /locus_tag="ACH3L1_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFT4075893.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(29158..29865)
                     /locus_tag="ACH3L1_001065"
     CDS             complement(29158..29865)
                     /locus_tag="ACH3L1_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFT4075894.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            30029..31006
                     /gene="ybeQ"
                     /locus_tag="ACH3L1_001066"
     CDS             30029..31006
                     /gene="ybeQ"
                     /locus_tag="ACH3L1_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFT4075895.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(31076..31558)
                     /locus_tag="ACH3L1_001067"
     CDS             complement(31076..31558)
                     /locus_tag="ACH3L1_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFT4075896.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            31793..34375
                     /gene="leuS"
                     /locus_tag="ACH3L1_001068"
     CDS             31793..34375
                     /gene="leuS"
                     /locus_tag="ACH3L1_001068"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFT4075897.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            34390..34971
                     /gene="lptE"
                     /locus_tag="ACH3L1_001069"
     CDS             34390..34971
                     /gene="lptE"
                     /locus_tag="ACH3L1_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFT4075898.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            34971..36002
                     /gene="holA"
                     /locus_tag="ACH3L1_001070"
     CDS             34971..36002
                     /gene="holA"
                     /locus_tag="ACH3L1_001070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFT4075899.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            36004..36645
                     /gene="nadD"
                     /locus_tag="ACH3L1_001071"
     CDS             36004..36645
                     /gene="nadD"
                     /locus_tag="ACH3L1_001071"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFT4075900.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            36669..37280
                     /locus_tag="ACH3L1_001072"
     CDS             36669..37280
                     /locus_tag="ACH3L1_001072"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFT4075901.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            37540..37857
                     /gene="rsfS"
                     /locus_tag="ACH3L1_001073"
     CDS             37540..37857
                     /gene="rsfS"
                     /locus_tag="ACH3L1_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFT4075902.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            37861..38328
                     /gene="rlmH"
                     /locus_tag="ACH3L1_001074"
     CDS             37861..38328
                     /gene="rlmH"
                     /locus_tag="ACH3L1_001074"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFT4075903.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            38359..40260
                     /gene="mrdA"
                     /locus_tag="ACH3L1_001075"
     CDS             38359..40260
                     /gene="mrdA"
                     /locus_tag="ACH3L1_001075"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFT4075904.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            40263..41375
                     /gene="mrdB"
                     /locus_tag="ACH3L1_001076"
                     /gene_synonym="rodA"
     CDS             40263..41375
                     /gene="mrdB"
                     /locus_tag="ACH3L1_001076"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFT4075905.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            41386..42474
                     /gene="rlpA"
                     /locus_tag="ACH3L1_001077"
     CDS             41386..42474
                     /gene="rlpA"
                     /locus_tag="ACH3L1_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFT4075906.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            42613..43824
                     /gene="dacA"
                     /locus_tag="ACH3L1_001078"
     CDS             42613..43824
                     /gene="dacA"
                     /locus_tag="ACH3L1_001078"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFT4075907.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            43935..44198
                     /gene="ybeD"
                     /locus_tag="ACH3L1_001079"
     CDS             43935..44198
                     /gene="ybeD"
                     /locus_tag="ACH3L1_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFT4075908.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            44299..44940
                     /gene="lipB"
                     /locus_tag="ACH3L1_001080"
     CDS             44299..44940
                     /gene="lipB"
                     /locus_tag="ACH3L1_001080"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFT4075909.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT        11218 a        11528 c        11454 g        10891 t
ORIGIN      
        1 ttcaatatat tgcagagatc atgtaggcct gataagcgaa gcgcatcagg caatttagcg
       61 tttgaaattt attgtctatt tattgccgga tgcgacgtaa atgcctcacc cggcttggta
      121 gaacttaaaa ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa
      181 ataccggttt ttcggccgtg gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa
      241 actggaatgc tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga
      301 tcaccagcga ttccgggtta atcaccgaca ggaaatcatc cgcagcacct gggttaggca
      361 cgctgaacag acgatcatac aaacggattt caaccggcag cgcatgtgcc gcgctcaccc
      421 agtgaataac acctttgact ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt
      481 cataagtaca gaagatggtg gtgatattac cttcggcatc tttctccacg cgttcagcct
      541 taatgacgta agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct
      601 tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat ttcaccgcta aacggcacct
      661 gacggctccc catttccggt ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc
      721 cctgatagtt ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggcgcat
      781 tttcgttgag atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct
      841 gcttggtcac gccgatgcgt ttgcagaact cacgaataga agccgcagtg taaccacgac
      901 gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg
      961 tcaccagcag gttcagctta cgcttggaca tcacagtgta ttccagattc aggcgcgaga
     1021 attcgtactg gcgcggatga acaggaatgg tgatgttatc cagtacccag tcgtacagac
     1081 gacggttgtc ctggaactca agcgtacaca gagagtgcgt aataccttcc agcgcatcgc
     1141 tgatgcaatg ggtgaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt
     1201 gttcagcaaa tttaatgcgg tacagcaccg gatcgcgcat tacgataaat ggcgacgcca
     1261 tgtcgatttt cgcacgcagg caggctttac cttcttcaaa accaccggca cgcatttttt
     1321 cgaacagcgc caagttctct tcaacgctgc ggtcgcggta cgggctgttt ttacccggct
     1381 gcgtcagggt accgcggtat tcgcggatct gttccggtgt cagttcatca acgtacgcca
     1441 ggcctttatt gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt
     1501 aacggacgtt accagaccag tgaaaaccta accactctac gtcgtttttg atcgaatcga
     1561 catactcgat atcttctttt accgggttag tgtcgtcgaa acgcaggttg cactggcctt
     1621 tatagtcctg ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat
     1681 agccattcgg ctccggcggg aaacgggtgt gtactgtgat gtgcttacca ctggccagat
     1741 cttcatcgat gatctgacgg ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg
     1801 attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac
     1861 aactgttagt tttttaaaat tgataatcta acaaggatta tggggatgat tgatgaaaaa
     1921 aaaagcggca gagaagatct ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc
     1981 atcaggcata aagcagatta ctttttgatt tcatacagcg gtgtttgacc cgccacaaca
     2041 tggccctgag ctttaatgag caagccactg aaatcgtcga tattgctgca aaccaccggg
     2101 ctaatcatcg agcgggcgtt agcgttcagg taatccagat ccatttccag aatcggttgc
     2161 cctgcgctta cctgcgcacc ctcttccacc agacgtttaa agcctttacc ttccagcgct
     2221 acggtgtcga tacccatatg gacgacgatc tccgcgcctt tttcggtttc caggcagaac
     2281 gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg ctggtgatac gacgatttta
     2341 tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc ttcgtcagga
     2401 acctgatcca gtgccacgac atcaccggta atcggcgata ccagctccgc gatagatacc
     2461 gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag ttgccggagt tgcttcagct
     2521 gacgcagcgg ctaccggacc acgggcaacg actttcttca tcgcatcgcc gatggattct
     2581 gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gtttcactac accagaagca
     2641 cccagacgtt tacacatcgt atcgttgacg cgggcagagt cagccacggt caggcgcaga
     2701 cgggtaatac aggcatcaat cgctttcagg ttgtcagtac cgccaaccgc agcaatatag
     2761 ttggttgcca gttgagtcag accttcttca gtattgctgt tggcttcttc agtaacgatc
     2821 tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat aaccaaactg
     2881 aacaccacga agtagatagc gaagaagata acgcccatca ccagcagcat ccagacgttc
     2941 tggctggcgg caggcaggtt atacatcaac gcgtagtcga tagcacccgc agagaaagag
     3001 aagccggcat ggatacccag cagcgttgcc acaaacaggc tgataccggt cagcagtgcg
     3061 tgcaggaggt acagcagcgg cgcaaggaac atgaacagga attccagcgg ctcagtcaca
     3121 ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat cggacgacgc
     3181 tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcagaccgaa catcatgatc
     3241 gggaagaagc cggacatgaa catccccgcg gtgccgtcac cagcgtagaa acggttgatg
     3301 tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac caatctggaa ccaggcgatg
     3361 gtgttcagca cctgatgcag accggttggg atcagcagac ggttgatgaa accaaagata
     3421 ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat agcgtgctgt
     3481 accggcggcc agacgtaacc aaaaatggcc gccagcacca gacagaagaa gccggtggcg
     3541 atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt aatatcggac
     3601 caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag tacacccatg
     3661 ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc taccgcaccc
     3721 gccagcgccg ccgcacctgc gctgtctttc gaccagctgg atgccacacc gatggcgaag
     3781 attaatgcga ggttatcaaa aattgcaccg cccgcctggg caataaacgc aacgttaagt
     3841 aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc gatagggagc
     3901 tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt ccccctacga
     3961 gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattt tactgccgat
     4021 gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt aattcgcatc
     4081 gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc atcactccct
     4141 tttactggct aaaccagaaa acttatttta tcattcaaaa aatcaagtcg gattgacgcc
     4201 tgtctgcgca aatccaggtt acgcttaaag atgtctaatc cgccaacggc ttacatttta
     4261 cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg tcggcaaatg
     4321 ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt
     4381 tgtactgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat
     4441 gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg acgaatatgt
     4501 cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta atttcttcga
     4561 tcacgttgat attccagcag aaaacatcaa ccttctcaac ggcaatgccc cggatatcga
     4621 tgccgagtgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc atctgtttat
     4681 gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt ctctggcttc
     4741 tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc gtttctttga
     4801 taacgatgtc aatcaggtgc caaaatatgc cctgactgtc ggtgttggta cactgctgga
     4861 tgccgaagaa gtgatgatta tggtgctggg tagccagaaa gcactggcgc tgcaggccgc
     4921 cgttgaaggt tgcgtgaacc atatgtggac catcagctgt ctgcaactgc atccgaaagc
     4981 gatcatggtg tgcgatgaac cttccaccat ggagctgaaa gttaagactt taagatattt
     5041 caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc cctggttcct
     5101 tgctcagggc aatatttttt aaaatcgggg ggcagaatgt atgcattaac ccagggccgg
     5161 atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga tggcctgatt
     5221 aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc actgaacggg
     5281 gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt
     5341 aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa agccaatgag
     5401 aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga gctgatgaaa
     5461 cagggcgtgc gcgttatgcg tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg
     5521 catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc gaattttgtg
     5581 cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa
     5641 gtgaccctgg caccggaaat ggttcctgcg gaagtcatca gcaaactggc aaatgccggg
     5701 attgtggttt ctgccggtca ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc
     5761 gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa
     5821 ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat tatcgctgat
     5881 ggcctgcatg ttgattacgc caacattcgt aacgctaaac gccttaaagg cgacaaactg
     5941 tgtctggtta ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt catttttgcg
     6001 ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac gttaagcggt
     6061 tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg
     6121 gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt
     6181 ctcggcacgc tcgccgcagg taaagtagcc aacctgaccg cattcacacc tgattttaaa
     6241 atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa agtatgacac
     6301 caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg
     6361 tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc gagcaaagcc
     6421 agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc gggctgatca
     6481 aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa
     6541 cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca tgacgccacc atcactctgt
     6601 ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa cgtacccagc
     6661 agacgctgga acatgccctg ctgaatgcca ttgctcagtt tattgatagc taccagcgca
     6721 agctgcgcga gctaatcgcc atttcggtga tcctgccagg gcttgttgac ccggacagcg
     6781 gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggta gaagcactgg
     6841 aagaacgttt taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg
     6901 agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt gtccatcgcg
     6961 gaaccggggc cgggattatc tctaacgggc gcatttttat tggccgcaac ggtaacgtcg
     7021 gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg
     7081 gctgcctgga aactatcgcc gccaacgctg ccattgaaca acgggtgttg aatctgttaa
     7141 agcagggcta ccagagccgt gtgccactgg acgactgcac catcaaaact atctgcaaag
     7201 ccgcgaacaa aggcgatagc ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg
     7261 gtaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt attgctggtg
     7321 aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt aatacccaag
     7381 cgctgaaggc gtttcgtact aatctgccgg tggtacgttc tgagctggat caccgctcgg
     7441 caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt
     7501 tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc
     7561 catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc
     7621 cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc cgctggtgtt
     7681 gctgaccaac tatccttcgc agactgggca agatctggcg aaccgctttg ccactgccgg
     7741 tgtcgatgtg ccggatagcg tgttttatac ctctgcgatg gcgaccgccg atttcctgcg
     7801 tcgctaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc atgaactgta
     7861 caaagccggt ttcactatta ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg
     7921 ttcctacaac tgggacatga ttcataaagc agcctatttc gtcgctaacg gtgcacgctt
     7981 tatcgccacc aacccggaca cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg
     8041 tgcagggatt gagaaaatct ccgggcgcaa accgttctat gttggtaagc ccagcccgtg
     8101 gatcatccgc gcggcattaa acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg
     8161 cgataacctg cgtaccgata ttctggcggg cttccaggcc ggtctggaga cgattctggt
     8221 gctttctggt gtttcatcgc tcgacgatat cgacagtatg ccgttccgcc caagctggat
     8281 ttacccgtca gtcgctgaaa tcgacgttat ctgaatacca cccacgtctg cggacgtggt
     8341 tggctctgat gacaaacgcc aaactgcctg atgcgctacg cttatcaggc ctacgcagct
     8401 cctgcaatat attgaatttt catgcttttg taggccggat aaggcgttca cgccgcatcc
     8461 ggcatgaaca aatcgcactt tatcagcaat ttggcccccg taaaatataa ccattcaata
     8521 aaacccgcta aaaattatcg attcatcaat aaatacatcc tcacaccaga ccctttttca
     8581 ccaatcgaca atcaccatta cgttttttat ttttgcactg ccaaatagct tgcgcacagt
     8641 gctgctttgc ggcatttttt taaacaagca aacacaacaa gcaacaaata ccaggttaac
     8701 ggagaaggtt atgtgttcaa tttttggcgt attcgatatc aaaacagacg cagttgagct
     8761 gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg
     8821 tatttatgcc agcgataacg ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa
     8881 cgcgggggcg caacctctct acaaccaaca aaaaaaccac gtactggcgg taaacggtga
     8941 aatctacaac caccaggcat tgcgcgctga atatggcgat cgttatcagt tccagacagg
     9001 gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga
     9061 cttgcagggc atgtttgcct ttgccctgta cgacagcgaa aaagatgcct acctgattgg
     9121 tcgcgaccat ctggggatca tcccactgta tatggggtat gacgaacacg gtcagctgta
     9181 tgtggcctca gaaatgaaat cgctggtgcc agtttgccgc acgattaaag agttcccggc
     9241 ggggagctat ttgtggagtc aggacggcga aatccgttct tattatcatc gcgactggtt
     9301 cgactttgat gcagtgaaag ataacgtgac cgacaaaaac gagctgcgtc aggcactgga
     9361 agattccgtt aaaagccatc tgatgtctga tgtgccttac ggtgtgctac tttctggtgg
     9421 tctggattcc tcaattattt ccgctatcac caagaaatac gcagcccgtc gcgtggaaga
     9481 tcaggaacgc tctgaagcct ggtggccgca gttacactcc tttgctgtag gtctgccggg
     9541 ttcaccggat cttaaagcag cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga
     9601 aattcacttc actgtacagg aaggtctgga tgccatccgc gacgtgattt accacatcga
     9661 aacttatgat gtgaccacta ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat
     9721 caaggcgatg ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg
     9781 ttatctttac ttccataaag cgcccaacgt taaagaactg catgaagaga cggtgcgtaa
     9841 actgctggcc ttgcatatgt atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg
     9901 cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa
     9961 cccgcaggat aaaatgtgcg gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt
    10021 tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt
    10081 cggttacagt tggatcgaca ccctgaaaga agtggcggcg cagcaggttt ctgatcagca
    10141 actggaaact gcccgcttcc gcttcccgta caacacgccg acctcaaaag aagcgtatct
    10201 gtaccgcgag atctttgaag aactgttccc acttccgagt gccgctgagt gcgtgccggg
    10261 cggtccttcc gtcgcgtgtt cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa
    10321 aatggacgat ccgtctggtc gtgcggttgg cgttcaccag tcggcgtata agtaagaaat
    10381 gcggtactgc cccggagaaa tccggggctg ttttagttat tgcgctgctt gttgattctg
    10441 atatgcagta aggaaatgtt ccgggtttgc cacatcactg tttgacaact catcactgta
    10501 ggccagtatt tcatagatta atttcccacc actattgttg aattccgaat ttcatcctgt
    10561 acgacaaaag aatggctgta gggtggggaa aaacacaaag taaacaataa ttgacgaata
    10621 tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa
    10681 agaggttgac gctgcaaggc tctatacgca taatgcgccc cgcaacgccg ataaggtatc
    10741 gcgaaaaaaa gatggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
    10801 tcacaggttc gaatcccgtc gtagccacca tcttttttgc gggagtggcg aaattggtag
    10861 acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca
    10921 ccattcacca gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt
    10981 gataccggca ttccctggtt cgaatccagg taccccagcc atcttcttcg agtaagcggt
    11041 tcaccgcccg gttattgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat
    11101 tccctggttc gaatccaggt accccagcca tcgaagaaat aatctggcta cgtagctcag
    11161 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
    11221 caaattctga atgtgtcgaa tacgttcggc aaattcaaaa accaatttgt tggggtatcg
    11281 ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc
    11341 agccacatta aaaaagctcg cttcggcgag ctttttgctt ttctgcgttc attcaatgtc
    11401 gaatgcgatg ttgacacgtc ttatccttca atgtcggatg cgacgctgcc gcgtcttatc
    11461 cgacctacgg ctggcacgca tccggcaatg ttgtaaggct acaaccctaa cgcatatttc
    11521 agcgcctgac gtttcaacac gccagcacgc tctgccgcca ttaacccgag attacgcaca
    11581 aaacgcagcg gcggcagatt attactgaat ccggcataaa acagatccat accactttgc
    11641 ataatgaagt tatccgccat gcgccgcatc tggtaacgct tgaggacagg ataactggcc
    11701 cacgcttcac cgtagctgcg ggcattcacc agaacatcaa tcagggcatc gacatcacga
    11761 taaccaagat tcaccccctg ccccgccagc ggatggatgg tatgcgcggc atcgcccacc
    11821 agcgcaagcc ccggctgcac atactgcaac gcatgtcggc gcgtcagcgg aaacgcacca
    11881 gcggcaagcg gtgtaacgta acccagacgc gacgggaaat gcttcgcgat ttccgcctgg
    11941 agttgcgcca tattcatatt ctgcaactgg cgaatacgcg tcggagagtc ataccacacc
    12001 agcgatgccc agttatcaaa caacggcaga aacgcacgcg gtccgtccgg ggtaaattgc
    12061 tgccaggtgc tgtcgccggg atcgttctca cactgtacgc taatcaacat gcacgactgc
    12121 gcatactgcc aggcatgaac gccaattccc gccatctgcc gcacctgaga atttgcgccg
    12181 tcggcaccaa tcaccagttt cgcgcggatc gtttcaccgc ccttcagttc cagctcctga
    12241 agatcattat ctcgatgtaa tgcaatcagc gaggttggca cacgtaacgt tactttcgga
    12301 tgcgcttcca gcgcctgcca caacgcctgt tgcaggactg tgttttccac catgtagcca
    12361 agcaacggca actttaattc ggctgcgtca aataccacat gtgccgtttc ccactcccag
    12421 gtttccagtc tgcggtaagg atggcaacgc atagcctgta ctgcatccca gacccctaac
    12481 cctttaagca atgataccga agccgcgctg atcgccgaga tccgcacgtc cggttggctg
    12541 tcagcgacaa acggtgcagg ttctgcgtgc tcgatcaccg ttaccgaaaa tccgtgctgt
    12601 gccagcccca gcgccagtgc gccgccgacc attcctccgc cgacaatggc aatttccgtt
    12661 ggttgatttg tcatggtcaa tcatcctgtt agcaatatgc tttaagttta caggattttt
    12721 acccccaagg gctgataacg ctcatactgg tcacaacggc agcaaagcat tacactatgc
    12781 gccctgcatt cctggctact atttcgcaag agcaagtcga tgaccaaaaa actccatatt
    12841 aaaacctggg gctgtcagat gaacgagtac gattcatcga agatggccga tctgctggat
    12901 gccacccacg gctatcaact gaccgacgtg gcggaagaag cggatgtgct actgctgaac
    12961 acctgctcaa tccgcgagaa ggctcaggaa aaagtcttcc atcagttggg tcgctggaaa
    13021 ctgttaaaag agaagaatcc agacctgatt atcggcgtcg gtggctgcgt ggcatcgcaa
    13081 gaaggcgagc acattcgcca gcgcgcccac tatgtcgata ttatttttgg gccgcaaacg
    13141 ctgcaccgtc tgccggagat gatcaactcc gtgcgcggcg accgcagccc ggttgtagat
    13201 atcagcttcc cggaaatcga gaagtttgac cgtctgccgg aaccgcgcgc cgaagggccg
    13261 accgcgtttg tctccatcat ggaaggctgc aataaatatt gcacctactg cgtggtgcct
    13321 tacacccgtg gtgaagaggt aagccgtccg tctgacgata ttctgtttga gattgcccag
    13381 cttgcggctc agggcgtgcg tgaagtcaac ctgctcggtc agaatgtgaa tgcctggcgt
    13441 ggtgagaact acgacggcac caccggatcg tttgccgatc tgttgcgtct ggttgctgcg
    13501 atcgacggga tcgatcgtat tcgctttacc accagccatc cgatcgaatt caccgacgat
    13561 atcatcgaag tgtaccgcga cacgcctgag ctggtgagct tcctgcatct gccggtacag
    13621 agcggttccg atcgcattct gaacctgatg ggacgtaccc atacggcgct ggagtacaaa
    13681 gcgatcatcc gtaaactgcg tgcggcgcgt ccggatattc agatcagttc tgacttcatt
    13741 gttggcttcc ctggcgaaac caccgaagac ttcgagaaaa cgatgaagct gattgccgac
    13801 gtcaatttcg acatgagcta cagctttatc ttctctgcac gtccaggaac gcctgccgcc
    13861 gatatggttg atgatgttcc ggaagaagag aagaagcagc gtctgtatat tctgcaagaa
    13921 cgtatcaatc agcaagcgat ggcatggagc cgtcggatgc tcggcaccac ccagcgtatt
    13981 ctggtagaag ggacttcgcg taagagcatc atggagcttt ccgggcgtac cgaaaataac
    14041 cgcgtggtca acttcgaggg cacaccggat atgatcggta aattcgtcga tgtagaaatt
    14101 accgacgtct acccgaactc tctgcgcggt aaagtggtgc gaactgaaga tgaaatgggt
    14161 ctgcgcatgg cagaaacacc ggagtcggtg attgcccgca cccgcaaaga aaacgacctt
    14221 ggcgtgggtt attatcagcc gtaattctca ggcctgccgt tccggtgggc cttgtttttt
    14281 ctctctttat ccccatcttt atcgcaatgc ttgcgccttt ttcctgtggc gtgaataatt
    14341 tccttatagg ccggtgaatg ttcagcttcc ggcggcatac agataagagg aacggtttga
    14401 acatagacac tcgcgaaatc accctggagc cagcagacaa tgcgcgtctg ttgagcctgt
    14461 gcggcccgtt tgatgacaac atcaagcagc tcgaacgccg tctcggcatc gagatcaatc
    14521 gccgcgataa ccactttaaa ctgaccggcc gtccgatttg cgtcaccgct gcggcagaca
    14581 ttctgcgtag cctgtatgtc gatactgccc cgatgcgcgg tcagattcag gatatcgaac
    14641 cggaacagat ccaccttgcg attaaagaag cgcgggtact ggagcaaagc gcggagagcg
    14701 tgccggagta cggcaaagcg gtcaatatca aaactaaacg cggcgtaatt aagccgcgca
    14761 cgccaaacca ggcgcagtac atcgccaata ttctcgacca tgacattacc ttcggcgttg
    14821 gcccggcggg tacgggtaaa acctacctgg cagtggctgc ggcagttgat gccctggagc
    14881 gtcaggaaat tcgccgtatt ctgctgactc gtccggcggt cgaagccggt gagaaactgg
    14941 gcttcctgcc tggcgattta agccagaaag tagacccgta tctgcgccca ctgtacgacg
    15001 cgctgtttga aatgctgggc tttgagaaag tcgagaaact gattgagcgc aacgttattg
    15061 aagtcgcgcc gctggcctat atgcgtggtc gtacgctgaa cgacgcgttt atcattctcg
    15121 atgagagcca gaacactacc atcgaacaga tgaagatgtt cctgacccgt atcggtttta
    15181 actcaaaagc ggttatcacc ggcgacgtca cacagatcga cctgccgcgt aatactaaat
    15241 caggcttacg tcatgccatt gaagtgctgg ccgatgtcga agagatcagc tttaacttct
    15301 tccacagcga agacgtggtt cgtcacccgg tggtggcgcg tatcgttaac gcttatgaag
    15361 cctgggaaga agcagaacaa aaacgtaaag cggcgctggc agcagaacgc aagcgcgaag
    15421 aacaggaaca aaaatgagtc aggtgatcct cgatttacaa ctggcatgtg aagataattc
    15481 cgggttaccg gaagagagcc agtttcaaac atggctgaat gcggtgatcc cgcagtttca
    15541 ggaagagtcg gaagtgacta ttcgcgtggt cgataccgcc gaaagccaca gtctgaatct
    15601 gacctatcgc ggtaaggata agccgaccaa cgtgctctcc ttcccgtttg aagtgccgcc
    15661 tggcatggag atgtcgctac tgggcgatct ggttatctgc cgtcaggtgg ttgagaagga
    15721 agctcaggag caaggcaaac cactggaggc gcactgggcg catatggtgg tgcacggcag
    15781 tctgcatttg ttaggttacg atcacattga agatgacgaa gcagaagaaa tggaagccct
    15841 cgaaacagag attatgcttg ctctgggcta tgaggatccg tacattgccg agaaagaata
    15901 atttgccagg catttgactg gcagacgtcc ccgccgttgg tgattaacga cggcgttttg
    15961 attaactaac atgagaaccc atacgacgcc atgagcgacg acaattcaca cagtagtgac
    16021 acgataagca acaagaaggg atttttctcc ctgttactca gccaactttt ccacggtgaa
    16081 ccgaaaaacc gtgacgaact gctggcgctg atccgtgatt ccgggcagaa cgaccttatc
    16141 gacgaagata cgcgcgatat gctcgaaggg gtgatggaca tcgcagacca acgcgttcgc
    16201 gacatcatga tcccccgctc ccagatgatt accctgaaac gcaaccagac gctggacgaa
    16261 tgccttgatg tcatcatcga gtccgcccac tcacgtttcc cggtgattag cgaagacaaa
    16321 gatcacattg aagggattct gatggcgaaa gacttgctgc cgtttatgcg cagcgatgct
    16381 gaagccttca gcatggacaa agtgttacgt caggcggttg tcgttcctga aagtaagcgc
    16441 gtagaccgga tgctgaaaga gtttcgctct cagcgttacc acatggcgat cgttattgac
    16501 gaattcggtg gggtttccgg tctggtgacc attgaagaca tcctggaact gattgttggt
    16561 gagattgaag acgagtatga cgaagaagat gatatcgact tccgtcagct gagtcgtcat
    16621 acctggaccg tgcgcgcact ggcttccatt gaagacttca acgaagcgtt cggcacccac
    16681 tttagcgatg aagaagtcga cactatcggt ggtctggtga tgcaggcgtt tgggcatctt
    16741 ccggcgcgtg gcgaaactat cgacatcgac ggttaccagt tcaaagtggc gatggccgat
    16801 agtcggcgta ttattcaggt tcatgtcaaa atcccggatg actcacccca gccaaagctg
    16861 gatgaataaa accgaaactg gatagataac tacatggatt ttgcctcatt aattgaacgc
    16921 cagcgcattc gcctgctgct ggcgttatta ttcggtgcct gcggaacgct ggccttctct
    16981 ccttacgacg tctggcctgc ggcgattatt tcgctgatgg ggcttcaggc gttgaccttt
    17041 aaccgtcgcc cactccagtc tgccgctatt ggcttttgct ggggatttgg cctctttggc
    17101 agcggtatta actgggtcta tgtcagcatc gcgacctttg gcgggatgcc tggcccggtt
    17161 aacatcttcc tggtggtgct gctggcggcg tatttgtcgc tgtataccgg actgtttgct
    17221 ggcgtgctgt cgcgtctgtg gccgaaaacc acctggctgc gcgtggcgat tgccacccct
    17281 gctctctggc aagtgaccga gtttctgcgc ggttgggtgc tgactggctt cccgtggtta
    17341 cagttcggct atagccagat tgatggcccg ttaaaagggc tggcaccgct aatgggcgtg
    17401 gaagccatta acttcctgct gatgatggtt agtggcctgc tggcactggc gttggtcaaa
    17461 cgcaactggc gtccgctggt ggtggccgtc gtgctgtttg ccctaccctt cccgctgcgt
    17521 tacatccaat ggtttacccc gcaaccggag aaaaccattc aggtttcgat ggttcagggc
    17581 gatattccgc aatcgctgaa atgggacgaa ggccagcttc ttaatacgct aaagatttac
    17641 tacaacgcaa cggcaccgct gatgggcaaa tcatcgttga ttatctggcc ggagtcggcg
    17701 ataaccgatc tggaaattaa tcagcaaccg ttcctcaaag cactggacgg tgagttgcgt
    17761 gataaaggta gctcgctggt aaccgggatt gtcgacgcgc gtctcaataa gcagaaccgc
    17821 tacgatacct acaacaccat catcacgctg ggtaaaggtg caccgtacag ctacgaatca
    17881 gccgatcgct ataacaaaaa ccatctggtg ccgtttggcg agtttgtccc gctggagtcg
    17941 attctgcgtc cgttagcacc gttctttgat ctgccgatgt cgtcgttcag ccgtgggcca
    18001 tatatccagc cgccgctgtc ggcaaatggt attgagctta ctgcggctat ttgctacgag
    18061 attattctcg ccgagcaagt gcgcgataac ttccgcccgg ataccgacta cctgctgact
    18121 atctccaacg atgcgtggtt tggtaaatct attggtccat ggcaacactt ccagatggcg
    18181 cgaatgcgtg cgctggagct ggcgcgccca ctgttgcgca gcaccaacaa cggcattacg
    18241 gcggtgattg gcccgcaggg tgagattcag gcgatgatcc cgcagttcgc ccgcgaggtg
    18301 ttaaccacta acgtgacgcc gaccaccgga ctcacaccat acgcacgtac cggcaactgg
    18361 ccgctgtggg tgctgacggc actgtttggt tttgctgctg tgttgatgag tctgcgtcag
    18421 cgacgtaaat aatcccttct tatcgtgccg gaatacatcc catatttcgg cacgctcacc
    18481 ggatgcggca tcagtgcatt atccggataa caatattctc ctatccaact ttggcacatc
    18541 tattgctttg ttatacaagg caaagcttga accagcatca gtacaacccg gttgcaccac
    18601 agcaatccac cggtagcacc aaaagagtgc attttaagaa ttttgcttca gaatggtgcg
    18661 gtgcacctcg caaaaacaaa caataaaaca tcaatatctt tacatttagc caaactaaat
    18721 gttacctaac aggcacaaca ctgcacaata aagttgcaga cgataacaac acaaacactc
    18781 acaacgggta tccatgcgtt cttaacgcag aagataaagg tgttggatat gcaattacgt
    18841 aaacctgcca cagcaatcct cgccctggcg ctttccgcag gactggcaca ggcagatgac
    18901 gccgccccgg cagcaggcag tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc
    18961 ggtcaccgtg aatcttcagt acctttctct tattacgaca atcagcaaaa agtggtgggt
    19021 tactcgcagg attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg
    19081 gacttgcagg taaaactgat tccgattacc tcacaaaatc gtattccact gctgcaaaac
    19141 ggcactttcg attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg
    19201 gctttctctg acactatttt cgtggtcggt acgcgcctgt tgaccaaaaa gggcggcgat
    19261 atcaaagatt ttgccgacct gaaaggcaaa gccgtagtcg tcacttccgg cactacctct
    19321 gaagttttgc tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc
    19381 aaagatcacg gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg
    19441 gatgacgctc tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggatatc
    19501 gtcggcaagc cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga cgatccgcag
    19561 ttcaaaaagc tgatggatga cactatcgct caggtgcaga cctccggtga agcggaaaaa
    19621 tggtttgata aatggttcaa aaatccaatt ccgccgaaaa acctgaacat gaatttcgaa
    19681 ctgtcagacg aaatgaaagc actgttcaaa gaaccgaatg acaaggcact gaactaatta
    19741 caagaaccag gggcggaaat tccagccctc tcgattgtta cgtagcacgg acagactata
    19801 cgcctgatgg tcgttcccca tcgggcctga aaaccgcaat acgctgggta acaatcttcg
    19861 agggtagcag ttaacgctgc taccctcttt tttctggagt agatttatgt ctatagactg
    19921 gaactggggt atttttttac aacaagcccc gttcggcaat accacctatc ttggttgggt
    19981 ctggagtggt tttcaggtga cgatcgcttt gtcgatctgc gcctggatta tcgctttcct
    20041 cgtcggttca tttttcggca ttttacgtac cgttccaaac cgttttctct ccggtctggg
    20101 tacgttgtat gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct ttacctggta
    20161 tctggtgatc ccggagctgc tgccggagaa aatcggcatg tggtttaagg ccgagctgga
    20221 tcccaatatt cagtttttcc tttcatccat gctctgcctg gggctgttta ccgccgcccg
    20281 tgtttgcgaa caggtacgcg ccgctattca gtcgctaccg cgtgggcaga agaatgccgc
    20341 gctggcgatg ggcctgacgc taccgcagac ttatcgatac gtactgctgc ctaatgctta
    20401 tcgcgttatc gtcccgccga tgacttccga gatgatgaac ctggtgaaaa actccgccat
    20461 cgcctcgacc atcggtctgg tggatatggc ggcgcaggcg ggtaaattgc tcgactactc
    20521 ggctcacgcc tgggagtcgt ttaccgccat cacgcttgcg tatgtgttga ttaacgcttt
    20581 tatcatgctg gtgatgacgc tggttgaacg taaagttcgc ctgcccggca acatgggggg
    20641 caaataatgt acgagtttga ctggagttcc attgttcctt ccctgccata tctgctcgac
    20701 gggctggtga tcaccctgaa aatcaccgtc acggcagtcg tgattggtat tttgtggggt
    20761 accatgctgg cggtgatgcg cttgtccagc ttcgcgcctg tttcgtggtt tgccaaagcc
    20821 tacgttaacg tattccgctc gattccttta gtcatggttt tgctgtggtt ttacttgatt
    20881 gtgccgggat ttctgcaaaa tgtgctggga ttatcgccaa aaaatgatat tcgtttaatt
    20941 tcggcgatgg tggcgttttc gatgtttgaa gcggcctact attcagagat tatccgtgcc
    21001 ggtattcaaa gtatttcccg tggtcagtcg agcgccgcgc tggcgttggg gatgactcac
    21061 tggcagtcga tgaaactgat tattctgccg caggctttcc gcgcgatggt gccgctgctg
    21121 ctcactcagg gcatcgtact gttccaggat acctcgctgg tgtatgtgtt atctctggcc
    21181 gatttcttcc gtaccgcctc aaccattggt gagcgtgatg gtacgcaggt cgagatgatc
    21241 ctgtttgccg gatttgttta tttcgttatt agccttagcg cgtcgttgtt ggtcagctac
    21301 ttgaaaagaa ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact
    21361 ttcaggtgct gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg
    21421 gcccgtctgg ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc
    21481 agcaaggtga aatcaccgtc gatggtatcg tggttaacga caagaaaacc gatctggcaa
    21541 agctgcgctc ccgcgtcggg atggtattcc agcatttcga gctgttccct catctgtcga
    21601 ttatcgaaaa cctgaccctg gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc
    21661 gtgaaaaagc attgaaattg ctggagcgcg tgggactttc cgcacatgcc aataaatttc
    21721 cggcgcagct ttccggcggt cagcagcagc gcgtggcaat cgctcgcgca ttgtgtatgg
    21781 ctcctattgc gatgctgttc gacgaaccga catcggcgct ggatccggag atgatcaacg
    21841 aagtgctgga cgtgatggtg gaactggcga acgaaggaat gaccatgatg gtggtgaccc
    21901 acgaaatggg ctttgcccgc aaagtggcga atcgggtgat ctttatggac gagggtaaaa
    21961 ttgtcgaaga ctcgccgaaa gacgctttct tcgatgatcc gaaatcggac cgcgcaaaag
    22021 acttcctcgc gaaaatcctg cattaatcac tctggcgcac atacccaatt gcgcgccatc
    22081 tcacgcttta tcgtttcgcc cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa
    22141 gaagcacaca acaaggagca acaatggcac tgccaattct gttagattgc gacccaggtc
    22201 atgatgacgc tatcgcaata gttctcgcac tcgcctcacc agagcttgat gtcaaagcaa
    22261 ttacgtcttc cgccggaaac cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc
    22321 tgaccttgct taatcgcacc gatattccgg tggcaggcgg cgcggtaaaa ccgttaatgc
    22381 gtgagttgat tatcgcggac aatgtgcacg gcgaaagcgg tctcgacggc ccggcattac
    22441 cggaaccgac gttcgcaccg caaaactgta cggcggtgga gctgatggcg aaaacgctgc
    22501 gcgaaagtgc ggaacctgtc acgattgttt ctaccggacc gcaaactaac gttgccttgc
    22561 tgctcaatag ccatccggaa ctgcatagca aaattgcccg tatcgtgatt atgggcggcg
    22621 caatggggct tggtaactgg acgcctgcgg ctgaatttaa tatttacgtt gacccggaag
    22681 cggcagaaat tgtcttccag tcagggatcc cggtggtgat ggccggtctg gatgttactc
    22741 ataaagcgca aatccacgtt gaagacaccg agcgtttccg cgcgattggc aaccctgttt
    22801 caaccattgt tgccgaactg ctggatttct tcctcgaata tcataaagac gaaaaatggg
    22861 gctttgtcgg cgcaccgctg catgacccat gcaccatcgc ctggctgttg aaacccgagt
    22921 tgtttaccac tgttgagcgc tgggttggcg tggaaacaca ggggaaatat acccagggta
    22981 tgacggttgt tgattattat tatctgactg gcaataaacc gaatgccact gtaattgtcg
    23041 atgttgatcg tcagggcttt gttgatttac tggctgatcg tctgaaattt tacgcttaac
    23101 actcgtcatt gattaagatc accacgcgtt acctgggtgg tcttaatcta tttatctctt
    23161 tttctttcaa gatataaatg caatggataa tgcagaactc gccataggta tcgatctcgg
    23221 cactaccaat agtttaattg ccgtctggaa agacggtgcc gcgcaattaa ttccaaataa
    23281 gttcggtgaa tatttaacac catccataat tagcatggat gaaaataatc atattttagt
    23341 cggaaaaccg gctgtatcac ggcgtacttc gcatccggat aaaacggcag cgttatttaa
    23401 acgtgcaatg ggcagtaata ccaactggcg gttaggcagc gacacattta acgcgccaga
    23461 actgtcctct ttggtattac gctcattaaa agaagatgcc gaagaatttc tgcaacgtcc
    23521 gattaaagat gtggtgatct ccgttctggc ttatttcagc gatgaacaac gcaagcatac
    23581 ccgtttagca gcggacttag ccgggttaaa tgcggtacgc ttaattaatg aacccacagc
    23641 agctgcgatg gcgtatggcc tgcataccca acaaaatacc cgttcgctgg tgtttgatct
    23701 cggtggcggc acgtttgacg ttacggtgct tgagtacgcc acgccggtga ttgaagttca
    23761 cgcctccgct ggcgacaact tcctcggtgg cgaagatttt acccatatgt tggtcgatga
    23821 cgttttaaag cgcgcggatg tcacaaggac cacgcttaac gagagtgaac tggcagcctt
    23881 gtacgcctgt gtggaagcgg caaaatgtag caatcaatcg ccaatgcaca ttcgctggta
    23941 gtaccaggat gaaacgcggg aatgcgaatt ttacgagaac gaactggaag atttgtggtt
    24001 gccgctgctc aatcgcttgc gagtgccgat tgaacaggcg ttgcgcgatg cgcgtctgaa
    24061 gccgagtcaa atcgacagtc tggtgctggt tggcggcgcg tcacaaatgc cgctggtgca
    24121 gcgaatcgcc gtgcgtctgt ttggcaaatt accgtatcaa agttacgatc cgagcaccat
    24181 tgtcgcgctg ggcgcagcaa tccaggccgc ctgccgctta cgcagtgaag atattgaaga
    24241 agtgatcctc actgatattt gcccttactc gttgggcgtt gaggttaacc gccagggcgt
    24301 ttccggcatt ttctcgccga ttattgaacg aaacaccact gtgcccgtgt cgcgtgtaga
    24361 aacttattca accatgcacc cggaacagga ttcaattacg gttaacgtct atcagggaga
    24421 aaaccacaaa gttaaaaaca acattctggt ggaatccttc gatgtgccgt tgaagaaaac
    24481 cggggcttat cagtcgattg atattcgctt tagttatgat atcaacgggt tgcttgaagt
    24541 tgacgtgctt ctggaagacg gcagcgttaa gtccagagtg attaaccaca gcccggtaac
    24601 attgagcgcg cagcagattg aagagagtcg gacgcggtta tccgcattga aaatttatcc
    24661 gcgcgatatg ctcatcaatc gcacctttaa agccaaactg gaagagttgt gggcgcgggc
    24721 gctgggtgac gagcgagaag agattggccg ggtgatcacc gattttgatg cggcgctgca
    24781 gtcaaacgat atggcccgcg tcgatgaggt caggcggcgg gcgagcgttt atttagccat
    24841 tgagacctca taatcacggc tgccggatgc ggcgtaaatg ctgcatccgg catcggcgca
    24901 aaaaacacat cgtcaactaa acaacatcgc gattttgccg agagcataac ctataaccag
    24961 caccgttaaa ctgattttga taatgctggt aatgctctgt ggtttttcac cctcatcatt
    25021 tttttcttca tggttttcag gtgttggctc atcgacatct tcatcctccg gctcgagttt
    25081 tactttcgtc aacagcatcc gggtgataat ggcctctaat ggatgagcat ctccgccttc
    25141 cagccttgta ttaagacggg ccagcatctc ttgtttcgga aacgcttcaa gtactacctg
    25201 ttccgctacc cgatacaaat ccagtaacgg tgaatcttct tctgtctcgc taccgactct
    25261 cgatttccac cacgcccatg ctttgcgttt gaagaataac gactcaataa acgattgacc
    25321 taacggcgat aattttccgc tatgtaaaat ttccgcccat aacagccgcg tccgggcgct
    25381 ctcaccagga acatatttca gcggttgacc ttgcgcatct accatgctcc gcgcttcgat
    25441 aaccatcact aataacgcga aataatccgg gcaatgacgc ttgctccact gcaacaacaa
    25501 attatcagct tgtgtagatg ggtaactttc ccagtacgcg ccgagatcag caagcaggct
    25561 ttccccctcg ccgcagtaca aacgttgtgc acactggtaa taccgggcat cttcgctttc
    25621 tggttcttcc gcttgccact gtttggctac aggaatgagt tccaccatac ctgtttgcac
    25681 cgagctgtac cagcgcaaca gtttgcgatg taactttgca tcatcaggaa tgaaccacgg
    25741 tccttccatc gccaaaaacg cagtgacata gtcaggatgg taacggcagc aacgttccag
    25801 cgcgaagtaa tattcaacag tctggttctg taacgcgacg ggatattcca gaaggctaag
    25861 gaaatcgaaa caatcaccag cctgaatggc ctcaagaaaa gcctcccttt cttcctcatc
    25921 cattccttcg ctgtcattaa acgaatgcca gttcaaacgc tctgccaaca aagaagcaca
    25981 atttaatgac agatatcgcg cttctatagc gattgcacac agcggccagc gtaattgatc
    26041 gacatcctcc atgttccagg tatttaattg ctgaataaat ttctgccagg cggagggctg
    26101 aaaacgatca ctttctgaat ccagtaatgt cctgaatgca cgtagtattt catgttcagc
    26161 ggtggcatct ttttcttcat catctgcttc ctcgacagga tttaccgcca gtcgcagcgc
    26221 ctcttcatag gcctggcgta atgctttaaa cccctgcgga tcggtttcgg gatgacacaa
    26281 cggtaagcga gcaagataag cctggcggat aatgtctatt tgcgtcgtgc tttcaatttc
    26341 gaggatttgc caacatgttt tcataggtcg ctcacatcct taagcgttac cgggcattca
    26401 ggctggacag ttaatggctg ccagggaagc gtggcataga aacggtcgct tcccgctgcc
    26461 agctcttcga acatccgact cccggcgcta tcgtatttgt acagggttag cgcctcgcgt
    26521 agcgaagcgt tgtcttcgca ctgagaagat tgccagtaga gacgccctag tgaataggca
    26581 gaaaaatagt gcatccagct gtgatagtaa tggtgagctc gtgcatagac gcgcgattgc
    26641 agccataaac tctcttcttc cgtcagccag ttgttgagca caccggcccg tgacagaaat
    26701 cccattcgaa cataatccca ggctttgatt cccccttctc cacagcacat agcggtttgg
    26761 gcaacgaatt gggtataaat caaccctctc tccgagccac cagcaattaa tgctttccac
    26821 tcttcagggg aagagcgttg ccatgtcaga tataagccag ctaaatcatt ggcatgacca
    26881 tcatcagtca tctgataaat agtctgaatt aaatcatcac gattatttat tccccaggat
    26941 gatgagagat cgaattcttt tatcccggta tacatttttg gcgagtcatg cgcgggtttg
    27001 caactctgat taagtatttc catgggcgca gaaagcgcaa aaagaagata ttgttcttcc
    27061 ttattcattt tttaataatc ccttaagtgc attaactgac tactttttta ttattgatga
    27121 gtataaactt gacgctttca aaataaaaag aaaatcgaag cattcacaca tgaataaaaa
    27181 attaatgtat atattcgcaa tttttatagt tgcagcaatt acctgtatta gccaacccaa
    27241 gaaaacgatg ttgcgtgata aagccatggt gaattatgcc tttgattatt taagatcacc
    27301 aggcagtcta ccgttaacca cggcagccac ggagctttcc gcgattcatg gtcactcaac
    27361 atcgcaatat cgccttggcg aattttatct tcatggtagc gacggtaaac cactggatta
    27421 tacacaggcg agatactggt atgagcaatc agcagaacag gaaaatccgc gcgcacaaag
    27481 caaactgggg tggatctacc tgaaaggcct gggagtcaaa cccgataccc gtaaagcaat
    27541 tctctggtat aaggaagcag ctgaacaagg gtatgctcat gctcaatata ctttgggttt
    27601 gatctacaga aatggcactg gtattaatgt taaccattat gaatctcaaa aatggttaaa
    27661 actggccgcc aaacaacatt acaaaaatgc ggaaagatta cttgccggac ttcccgcaca
    27721 ttaacgtcga ctgtcacccg aagagatgga gtattttccc tatcaaaccg gcaaagataa
    27781 aaatgtagaa aataattttg atgacgccca gacagcccag cccgccatca tcaacgggca
    27841 ttggctgcgg ttgcggctcc ggctcgacgg tttcctgacc ggtaaaattc gccgcctgaa
    27901 ttaataatcg acgaataatt aatgcttcca gtggacaacg atcttctgca tcaaggcggc
    27961 tcacaatcgg cattaatgat tcttctgtcg gttggctggc ctgcactaat tgtttggcaa
    28021 ctaaatataa tggccataag ggatgttctg tatcacgcgt gagtgagatc cgcggcttac
    28081 gtcctttgtg caaaaccatc tcgaccagtg aacgccccag cgcagagagt ttttcatcgc
    28141 tgagtatttc gtataaatga aaacgcgtac gggcgctgtc gccggggaca taaagtagcg
    28201 gttttccctg atcgttaacc agatcacgcg cttcaatcat catcaccact aatgggtaat
    28261 aatagacccg atgctgacgg caccattgca acattaaagc atccgcctgg gtggagggat
    28321 actcgcgcca gtagtaacaa agttctgcca gcaggctttc gccttctcca caatagacgc
    28381 gttgtgcgta ttcataataa cgcgcatctt cattatcagg ttcagcttcc cgccacgacc
    28441 gggcgtaatc caggagttcg gggattccca ggcggcttgc gctataccag cggagtaaat
    28501 ttctgtgcag ttttgcatca tcggggataa tcactgcgcg atgttgcgct aaatacaatg
    28561 tcagccattc tggatgcgat gaccaaatcc gagcgaacat ttggtaaaaa tcgatggtgt
    28621 cattttgcac ggcaactggc agatgcagaa tggtctggaa gttaaaaaca ttccccttcg
    28681 caatggcata taaaaaggat tccagttctt cttcgtctat ttccccgacg ttttcctcct
    28741 gcaaccaccg caatctttct gctaataaca ccacgcactc gaaggataaa tgggcagtgt
    28801 tcatggcgat tgtgcacagc gaccagcgta attcatcaat ctcttccatc gagcaataat
    28861 ttaattgctg aatgaatcgc tgccaggcgg agggcagaaa acgttcgcta tcagaggcaa
    28921 gtaacgcgcg aaaggcgagc agaatttcat gttctgctac ctcatattct tctggttgcc
    28981 aaacagattt agcaggcgac tgcgcaatcc gtagcgcctc ctcatacgcc tgacgaagtt
    29041 gtttaaaacc ctgcggatcg gtttctggat gaaaggacgg taacagcgcc agataagcgc
    29101 ggcggataat atcgacgtca gtcgtttcct ctatatcgag gatcttccag caatttttca
    29161 taagtcgctc atatccttaa gcgtttccgg gcatgtgggg tagtcagcca gtggttgcca
    29221 ggacaaggta gggtaatacg catcatcact ggcaaataat tgataaaaca tatcattgcc
    29281 ggaatcgtcg tattctttac gcgctaagaa ttcgcgtaat aaaggcagat gatcatcttc
    29341 gggagactgc caatattgcc gaccaaaggt ataaccggca aagtattgcc gccagttact
    29401 ataataacgt agagcccgta gatgaatacg cgactggatc catagacttt cttcttcact
    29461 caaccagtta ttaagtacgc ccattcgact taaaaaaccc atccggacat aatcccatgc
    29521 tttaattcct ccctcaccac agcattcggc tgtgctggcg acaaattgcg cataagcctg
    29581 accttgctca tttaattcag caagatagtc acgccattca catggagaat aacgaaacca
    29641 gcggtgataa aaccctgcca accgagcggc atgaccgttc tcgatcattc ggtgaattat
    29701 gcgcagcagg tcatcacggt catcaattcc ccatgaggaa gaaaggtcaa aaaagcatgt
    29761 acctaaatac attttggggg atgaatgcga aggtaaacag cattcattgc gtatttccat
    29821 cggagtggaa agtgcaaaca atattttttg cgaccccatg tccatataat ttatccttaa
    29881 acattcattt aatgaatgta aactacattt cgaggctcag ttatttatac ttttctgacg
    29941 caacataata cgcataaaat aaggcttacg caaaaatgga acacataaaa atataatata
    30001 ttttatctac aaccataagg aaatgataat gatttttacg tcaagttgct gcgataattt
    30061 atcaatagat gagattatcg aacgtgctga aaaaggcgat tgcgaggctc aatatattgt
    30121 tgggttttat tataatcgcg atagcgcaat tgactctccg gacgacgaaa aggcctttta
    30181 ctggctgaag ctggccgctg agcaaggtca ttgtgaagca cagtattcct tagggcaaaa
    30241 gtataccgag gataaaagcc gtcataaaga taatgagcaa gccatcttct ggctgaaaaa
    30301 agctgcccta caaggccata ctttcgcttc caacgccctt ggctggatac tggatcgtgg
    30361 agaagacccc aactataaag aagcggttac ctggtatcag atagccgcgg agagcggaat
    30421 gtcttatgcg caaaataatc ttggatggat gtacagaaat ggcaacggag tcgcacaaga
    30481 ctatacgctg gcatttttct ggtacaaaca agctgcatta caaggccata gttacgcaca
    30541 agacaatctg gccgatcttt ataaagacgg agaaggcgtt gctcaaaaca agacactcgc
    30601 cgcattctgg tatttgaaaa gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc
    30661 atgggattat aacgctggcg aaggggtgga ccaggactat aagcaagcga tgtactggta
    30721 tctgaaggct gccgctcagg aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa
    30781 acacggacaa ggcgttgaga aagattatca ggctgccttt gaatggttta cgaaagccgc
    30841 tgaatgcaat gacgccactg cctggtataa cctggccatt atgtatcact acggagaagg
    30901 aagacctgtc gatctccgac aggctctcga cctgtatcgt aaagttcagt catccggaac
    30961 cagagatgtc agtcaggaaa ttcgtgagac tgaagcttta ctgtagaaat aaaaacaagg
    31021 gccggggaat ttcattctgc ccatattctc tttattgctg tgcgaaatca gactattacg
    31081 gctcaaacgg gcgtctctgg aactggtcat aaccacattt cgggcatagc gtcagcactt
    31141 ccggtgtgta gatcgggaga tggaagtgac atttctcgca gaccagattt cccagcccga
    31201 ccacttctcc gctgtgataa accccatgat gattgaggtc ctggaaaatt tcgcgccatt
    31261 caagctgcgt tttatcggtg atgtctgcca gctcctgcca caagctttct ttaatcaccc
    31321 gcataaagac gctgtcagat tcttctttca ggctctcttc atagctcatg gcgaactctt
    31381 ccaggtcacg tctgacagct cgcgtcagct catcgatctc ggttcgcgtt aactcccctg
    31441 tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc
    31501 gcaggcgttc gttcagtgac gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg
    31561 tttcatctcc cgggtcgtta actgactata ataatagacg ttctttgttc cttgctttgc
    31621 taatacgccc acaaactctg taaattaatg tacgaataat ccgggcatca tttcaacgtc
    31681 gagtgcgcga aaggctgttt tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa
    31741 ccacctcaac gctacatttg tagccgtatt gaaaacagga ccactggctg ccatgcaaga
    31801 gcaataccgc ccggaagaga tagaatccaa agtacagctt cattgggatg agaagcgcac
    31861 atttgaagta accgaagacg agagcaaaga gaagtattac tgcctgtcta tgcttcccta
    31921 tccttctggt cgactacaca tgggccacgt acgtaactac accatcggtg acgtgatcgc
    31981 ccgttaccag cgcatgctgg gcaaaaacgt tctgcagccg atcggctggg acgcgtttgg
    32041 tctgcctgcg gaaggcgcgg cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga
    32101 caacatcgcg tatatgaaaa accagctcaa aatgctgggc tttggttatg actggagccg
    32161 cgagctggca acctgtacgc cggaatacta ccgttgggaa cagaaattct tcaccgagct
    32221 gtataaaaaa ggcctggtat ataaaaagac ttctgcggtc aactggtgcc cgaacgatca
    32281 gaccgtactg gcgaacgaac aagttatcga cggctgctgc tggcgctgcg ataccaaagt
    32341 tgaacgtaaa gagatcccgc agtggtttat caaaatcact gcttacgctg acgagctgct
    32401 caacgatctg gataaactgg atcactggcc agacaccgtt aaaaccatgc agcgtaactg
    32461 gatcggtcgt tccgaaggcg tggagatcac cttcaacgtt aacgactatg acaacacgct
    32521 gaccgtttac actacccgcc cggacacctt tatgggttgt acctacctgg cggtagctgc
    32581 gggtcatccg ctggcgcaga aagcggcgga aaataatcct gaactggcgg cctttattga
    32641 cgaatgccgt aacaccaaag ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt
    32701 cgatactggc tttaaagctg ttcacccatt aacgggcgaa gaaattcccg tttgggcagc
    32761 aaacttcgta ttgatggagt acggcacggg cgcagttatg gcggttccgg gtcacgacca
    32821 gcgcgactac gagtttgcct ctaaatacgg cctgaacatc aaaccggtta tcctggcggc
    32881 tgacggctct gagccagatc tttctcagca agccctgact gaaaaaggcg tgctgttcaa
    32941 ctctggcgag ttcaacggtc ttgaccatga agcggccttc aacgccatcg ccgataaact
    33001 gactgcgatg ggcgttggcg agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc
    33061 tcgccagcgt tactggggcg cgccgattcc gatggtgacg ctggaagacg gtaccgtaat
    33121 gccaaccccg gacgaccagc tgccggtgat cctgccggaa gatgtggtga tggacggcat
    33181 taccagcccg attaaagcag atccggagtg ggcaaaaact accgttaacg gtatgccagc
    33241 gctgcgtgaa accgacactt tcgacacctt tatggagtcc tcctggtact atgcgcgcta
    33301 cacttgccca gagtacaaag aaggtatgct ggattccgaa gcggctaact actggctgcc
    33361 ggtggatatc tacattggtg gtatcgaaca cgccattatg cacctgctct acttccgctt
    33421 cttccacaaa ctgatgcgtg atgcaggcat ggtgaactct gacgaaccag cgaaacagtt
    33481 gctgtgtcag ggtatggtgc tggcagatgc cttctactat gttggcgaaa acggcgaacg
    33541 taactgggtt tccccggttg atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa
    33601 agcgaaagat gcggcaggcc atgaactggt ttataccggc atgagcaaaa tgtccaagtc
    33661 gaagaacaac ggtatcgacc cgcaggtgat ggttgaacgt tacggcgcgg acaccgttcg
    33721 tctgtttatg atgtttgctt ctccggctga tatgactctc gaatggcagg aatccggtgt
    33781 ggaaggggct aaccgcttcc tgaaacgtgt ctggaaactg gtgtacgagc acacagcaaa
    33841 aggtgatgtt gcggcactga acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg
    33901 cgatgtgcat aaaactatcg ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa
    33961 caccgcaatt gcggcgatta tggagctgat gaacaaactg gcgaaagcac cgaccgatgg
    34021 cgagcaggat cgcgctctga tgcaggaagc actgcaggcc gttgtccgta tgctcaaccc
    34081 gttcaccccg cacatctgct tcacgctgtg gcaggaactc aaaggcgaag gcgatatcga
    34141 caacgcgccg tggccggttg ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt
    34201 ggtgcaggtt aacggtaaag tccgtgccaa aatcaccgtc ccggtggacg caacggaaga
    34261 acaggttcgc gaacgtgctg gccaggaaca tctggtagca aaatatcttg atggcgttac
    34321 tgtacgtaaa gtgatttacg taccaggtaa actcctcaat ctggtcgttg gctaagcgcg
    34381 ggaggaagcg tgcgatatct ggcaacactg ttgttatctc tggcggtgtt aatcaccgcc
    34441 gggtgtggct ggcatctgcg tgacaccacg caggttcctt ccactatgaa ggtcatgatc
    34501 ctggactcag gcgatccgaa cgggccatta agccgtgcgg tgcgtaacca gttacgtctg
    34561 aatggtgtcg agttgcttga taaagaaacc acgcgtaagg acgttccatc cctgcgtttg
    34621 ggtaaagtga gcatcgcgaa agataccgca tcggtattcc gtaacggtca aacagcagag
    34681 tatcagatga tcatgacggt gaatgcgacc gtgttgatcc ccggccgtga tatctacccg
    34741 attagcgcca aagtcttccg ttcattcttc gataacccgc aaatggcgtt agcgaaagat
    34801 aacgaacaag acatgatcgt aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt
    34861 aagctgccaa gcatccgtgc tgcggatatt cgttccgacg aagaacagac gtcgaccaca
    34921 acggatactc cggcaacgcc tgcacgcgtc tccaccatgc tgggtaactg atgattcggt
    34981 tgtacccgga acaactccgc gcgcagctca atgaagggct gcgcgcggcg tatcttttac
    35041 ttggtaacga tcctctgtta ttgcaggaaa gccaggacgc tgttcgccag gtagctgcgg
    35101 cacaaggatt cgaagaacac cacacttttt ccattgatcc caacactgac tggaatgcga
    35161 tcttttcgtt atgccaggct atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt
    35221 taccagaaaa cggaccgaat gcggcgatca atgaacaact tctcacactc accggacttc
    35281 tgcatgacga cctgctattg atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg
    35341 ccgcctggtt tactgctctt gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc
    35401 aggctcagct tccccgctgg gttgctgtgc gcgcaaaaca gctcaactta gaactggatg
    35461 acgcggcgaa tcaggtgctc tgctactgtt atgaaggtaa cctgctggcg ctggctcagg
    35521 cactggagcg tttatcgctg ctctggccag acggcaaact gacattaccg cgcgttgaac
    35581 aggcggtgaa tgatgccgcg catttcaccc cttttcattg ggttgatgct ttgttgatgg
    35641 gaaaaagtaa gcgcgcgttg catattcttc agcaactgcg tctggaaggc agcgagccgg
    35701 ttattttgtt gcgcacatta caacgtgaac tgttgttact ggttaacctg aaacgccagt
    35761 ctgcccatac gccactgcgt gcgttgtttg ataagcatcg ggtatggcag aaccgccggg
    35821 gcatgatggg cgaggcgtta aatcgcttaa gtcagccgca gttacgtcag gccgtgcaac
    35881 tcctgacacg aacggaactc accctcaaac aagattacgg tcagtcagtg tgggcagagc
    35941 tggaagggtt atctcttctg ttgtgccata aacccctggc ggacgtattt atcgacggtt
    36001 gatatgaaat ctttacaggc tctgtttggc ggcacctttg atccggtgca ctatggtcat
    36061 ctaaaacccg tggaaacgct ggcgaatttg attggtctga cgcgggtcac aatcatccct
    36121 aataatgttc ctccgcatcg tccccagccg gaagcgaaca gcgtgcagcg taaacacatg
    36181 cttgaactgg cgattgccga caagccatta tttactcttg atgaacgcga gctaaagcgc
    36241 aatgccccct cttacactgc gcaaacactg aaagagtggc ggcaggaaca aggaccggac
    36301 gtgccgctgg cgtttattat tggtcaggat tcattgctga cctttccgac ctggtacgaa
    36361 tacgaaacga tactcgacaa tgcacatttg atcgtctgtc ggcgtccagg ttacccactt
    36421 gaaatggcgc aaccgcaata ccagcaatgg ctggaagatc atttgacaca taacccggaa
    36481 gatcttcacc ttcagcctgc cggtaaaatt tatctggctg aaacgccgtg gtttaacatc
    36541 tcggcgacca tcatccgcga acgtttgcaa aacggtgaat cgtgtgagga tttattgccg
    36601 gaaccggtat tgacttacat taaccaacaa ggcttgtatc gctgatacct gtcgttacgg
    36661 agggcatgat gcgactgtgg ttaattcgtc atggcgaaac acaagcgaat gtcgatggtc
    36721 tgtacagcgg tcatgcgccc acacccctga ccgcacgcgg tattgagcaa gcgcaaaatc
    36781 tgcatacact gctagatgat gtttcctttg acttggtttt atgcagtgaa ctggaacggg
    36841 cacagcatac cgcgcgactg gttctcagtg accgccagct ccccgttcat atcatccctg
    36901 aactcaacga aatgtttttt ggcgactggg agatgcgaca tcatcgcgac ctcatgcaag
    36961 aagatgccga aaactatagc gcgtggtgca atgactggca gcatgctatc cccacgaacg
    37021 gtgaaggatt tcaggcattt tcgcaacgtg tggaacgctt tatcgcaagg cttagtgaat
    37081 atcagcacta tcagaatatt ttaatcgtca gccatcaggg cgtactgagt ctgttaattg
    37141 ctcgtttaat tggcatgcct gccgagtcta tgtggcattt tcgcgttgac caggggtgtt
    37201 ggagcgccat tgatatcaac caaaaattcg cgacgctacg cgtcctcaat agccgtgcca
    37261 tcggggtcga gaacgcatga cttttctgtt tttttacggg taagccgcaa cgaccattga
    37321 cagccccggg caggctgata ttctccgcag ccagactttt tccgccagac acgactttgt
    37381 agaaattgtt ttacaaaaat ggcgatgcaa tctgcggcgc ggggtgggat gatagcccac
    37441 tttcgaaagc cgattcggcg acaattgtcc cgaaatcgcc tctggttcag gtatactgac
    37501 agaccatttt tatctatttg attcacccag ggggaaaact tgcagggtaa agcactccag
    37561 gattttgtta tcgacaaaat tgatgacctc aaaggtcagg acatcatcgc cttagacgtt
    37621 caaggcaaat ccagcatcac cgactgcatg atcatctgta cgggtacgtc cagccgtcat
    37681 gttatgtcca ttgctgacca cgttgtgcag gagtctcgcg cagcgggcct gttaccgctc
    37741 ggcgtagaag gtgaaaacag cgccgactgg attgtcgtgg atttgggcga tgtgattgtc
    37801 catgtcatgc aggaagagag ccgtcgcctg tatgaactgg aaaaactctg gagttaatgc
    37861 gtgaagctgc aacttgtcgc cgtgggaacg aaaatgccgg actgggtaca aaccggtttt
    37921 accgagtacc tgcgtcgttt tccgaaagat atgcccttcg agctgattga aattccggcc
    37981 ggcaaacgcg gcaagaatgc ggacatcaag cgcatactcg acaaagaggg tgagcagatg
    38041 ttggcggccg caggcaaaaa ccgcattgtc accctcgata ttccaggcaa gccctgggat
    38101 acgccgcagt tagccgctga gctggaacgc tggaagctgg atggtcgcga cgtaagtcta
    38161 ctgattggcg ggcctgaagg gttgtcgcct gcctgtaaag cggcggctga gcagagctgg
    38221 tcgctgtcgg cgcttaccct cccccatccg ctggttcgcg tgctggtcgc agagagtctg
    38281 taccgggcgt ggagcatcac caccaaccat ccttatcacc gtgagtgata agggagcttt
    38341 gagtagaaaa cgcagcggat gaaactacag aactcttttc gcgactatac ggcagagtcc
    38401 gcgctgtttg tgcgccgggc gctggtcgcc tttttgggga ttttgctgct gaccggcgtg
    38461 cttatcgcca acctgtataa tctgcaaatt gttcgcttta ccgactacca gacccgctct
    38521 aatgaaaacc gcattaagct ggtgcctatc gcgcccagcc gcggcattat ctacgaccgt
    38581 aacggtatcc ctctggccct caaccgcact atctaccaga tagaaatgat gccggagaaa
    38641 gtcgataacg tgcagcaaac gctggacgct ttgcgcagcg tggtagatct gaccgatgac
    38701 gatattgctg cattccgaaa agagcgcgca cgttcacacc gtttcacctc tattccggtg
    38761 aaaactaacc taaccgaagt acaagtagct cgctttgccg tcaatcagta ccgttttccg
    38821 ggtgtcgaag ttaaaggcta taaacgtcgt tactatcctt acggttcggc gttgacccac
    38881 gtcatcggct atgtgtcgaa aatcaacgat aaagacgtcg aacgcctgaa taatgacggc
    38941 aaactggcca actatgcggc aacgcatgat attggtaagc tgggcattga gcgttactat
    39001 gaagacgtgc tgcacggtca gaccggttat gaagaggttg aagttaacaa ccgtgggcgt
    39061 gttatccgcc agttaaaaga agtaccaccg caagccggac acgatattta cctgacgctg
    39121 gatctcaaac tccagcaata tattgaaacg ctgctggcgg gtagccgcgc agctgtggtt
    39181 gttaccgaac cgcgtacagg tggggtgctg gcgctggttt ccacgcctag ttatgaccca
    39241 aacctgtttg ttgacggtat ctccagcaaa gattattccg ccttgctgaa cgacccgaat
    39301 acaccgctgg tgaaccgcgc cacacagggg gtttatcctc ccgcgtctac agttaaaccc
    39361 tatgtggcgg tttcggcatt aagcgccggg gtgatcacgc gcaatacgac gctgtttgac
    39421 ccaggctggt ggcaactacc agggtcggaa aaacgttatc gtgactggaa aaaatggggc
    39481 cacgggcgtc tgaatgtcac aagatcgctg gaagaatctg cggatacctt cttctatcag
    39541 gtggcctacg atatggggat cgatcgcctc tccgaatgga tgggtaaatt cggttatggt
    39601 cattacaccg gtatcgacct ggcggaagaa cgttccggca acatgcctac ccgcgaatgg
    39661 aaacagaaac gctttaaaaa accgtggtat cagggtgaca ccattccggt tggtatcggc
    39721 cagggttact ggacagcgac cccaatccag atgagtaagg cactgatgat cctgattaat
    39781 gacggtatcg tgaaggttcc gcatttgctg atgagcaccg ccgaagacgg caaacaggtg
    39841 ccatgggtac aaccgcatga accgcccgtc ggcgatattc attccggtta ctgggagctg
    39901 gcgaaagacg gtatgtacgg tgttgctaac cgccctaatg gtacggcgaa taaatacttt
    39961 gccagcgcac cgtacaaaat tgcggcgaaa tctggtaccg ctcaggtctt cggtctgaaa
    40021 gcgaacgaaa cctataatgc gcacaaaatt gccgagcgtt tacgtgacca caaactgatg
    40081 accgcctttg cgccatacaa caatccgcaa gtggctgtcg ccatgattct ggagaacggc
    40141 ggcgcgggtc cggcggttgg tacgctgatg cgccagatcc tcgaccacat tatgctgggt
    40201 gataacaaca ccgatctgcc tgcggaaaat ccagcggttg ccgcagcgga ggaccattaa
    40261 tcatgacgga taatccgaat aaaaaaacat tctgggataa agtccatctc gatcccacaa
    40321 tgctgctgat tttactggca ttgctggttt acagcgccct ggttatctgg agcgccagcg
    40381 gtcaggatat tggcatgatg gagcgtaaaa tcggccaaat cgcgatgggt ctggtcatca
    40441 tggtggtgat ggcgcaaatt cctccacgcg tttatgaagg ctgggccccc tatctctata
    40501 tcatctgtat tattttgctg gtggcggtag atgctttcgg agccatctct aaaggcgctc
    40561 aacgctggct ggacctcggt attgttcgtt ttcagccgtc ggaaattgcc aaaatagccg
    40621 taccactgat ggttgcgcgc tttatcaacc gcgacgtttg cccgccatcg ttgaagaata
    40681 ctggcatcgc gctggtgctg atatttatgc ccacgctgct ggtggctgca cagcctgacc
    40741 tgggaacatc aatcctcgtt gcgctttccg gtctgtttgt actgttcctc tctggcctta
    40801 gctggcgtct gattggcgtc gcagtagtgc tggtagcggc gttcattccg attctgtggt
    40861 tcttcctgat gcatgattac cagcgccagc gcgtaatgat gctcctggac ccggaatcag
    40921 acccactcgg cgcgggctat cacattattc agtctaaaat tgctattggc tccggcggat
    40981 tacgcggcaa aggctggctg cacggcactc agtcacagct tgaatttctc cccgaacgcc
    41041 atactgactt tatcttcgcg gtactggcgg aagagctggg attagtgggc attctgattc
    41101 tgctcgctct ctacattctg ctgatcatgc gcgggctgtg gatagccgcc agagcgcaaa
    41161 ccacctttgg tcgcgtcatg gctggtggct taatgctgat attattcgtt tatgtcttcg
    41221 taaatattgg tatggtaagc ggtattctgc cggttgtagg ggttccgctc ccactggtca
    41281 gttatggagg atcggcgcta attgtgctga tggctgggtt cgggattgta atgtcaatcc
    41341 acacccacag gaaaatgttg tcgaaaagcg tgtaagaggt gcgcaatgcg taagcagtgg
    41401 ctcgggatct gcatcgcggc aggaatgctc gcggcatgta caagcgatga tggtcagcaa
    41461 cagacggtaa gtgtaccgca gcctgcggta tgtaacggcc ccatagttga aattagcggg
    41521 gcggacccgc gtttcgaacc actgaacgcg acggcaaatc aggattacca gcgcgacggt
    41581 aaaagctaca aaatcgtgca ggatccgtct cgatttagcc aggcgggact ggcggcaatc
    41641 tatgatgccg aaccaggcag taacctgacg gcctctggcg aagctttcga tccgacaaag
    41701 ctgacggcgg cccatccaac gcttccgatc cccagctacg ccagaatcac taacctggct
    41761 aacgggcgaa tgatcgtggt gcgcattaat gatcgcggcc cttacggcaa cgaccgcgtt
    41821 atttcgcttt ctcgcgcggc agctgaccgt cttaacacgt caaacaacac caaagttcgt
    41881 atcgatccga ttattgtcgc ccaggatggt tcgctttctg gtcctggtat ggcttgtacc
    41941 acagtcgcca aacagactta cgccctgcct gcacctcccg atttaagcgg tggcgcggga
    42001 acaagttcag tgtctggccc gcagggtgac attcttccgg tcagtaattc gacgctaaaa
    42061 agcgaagatc cgaccggcgc gccggtaacc agtagcggtt tcctcggcgc tccaacgacc
    42121 ttagcgcctg gtgtactgga aggcagcgaa ccgacgcctg cgccacagcc cgttgttaca
    42181 gcttcgtcga caacgcctgc aacctcgcct gcaatggtga caccgcaagc cgcctcgcaa
    42241 agcgccagcg gcaactttat ggtgcaagtc ggggccgtaa gcgatcaggc tcgtgcgcaa
    42301 cagtaccaac agcaactggg acagaagttc ggcgtccccg gtcgcgtaac tcaaaatggc
    42361 gcggtctggc ggatccagct tggcccattc gccagcaaag ccgaagccag taccttgcag
    42421 caacgtttgc aaaccgaagc ccaattacag tcatttatta ctaccgcgca gtagcgtaaa
    42481 gcaggcatct gatgtgtcaa tttctgtaaa tgacgttaac aaagtcacgc ggaaagtcag
    42541 atgcctgccg gtagtgcatt tgctatagta gggcactttt ttaattccat cacggatgtc
    42601 gtagttcaga ccatgaatac cattttttcc gctcgtatca tgaagcgcct ggcgctcacc
    42661 acggctcttt gcacagcctt tatctctgct gcacatgccg atgacctgaa tatcaaaact
    42721 atgatcccgg gtgtaccgca gatcgatgcg gagtcctaca tcctgattga ctataactcc
    42781 ggcaaagtgc tcgccgaaca gaacgcagat gtccgccgcg atcctgccag cctgaccaaa
    42841 atgatgacca gttacgttat cggccaggca atgaaagccg gtaaatttaa agaaactgat
    42901 ttagtcacta tcggcaacga cgcatgggcc accggtaacc cggtgtttaa aggttcttcg
    42961 ctgatgttcc tcaaaccggg catgcaggtt ccggtttctc agctgatccg cggtattaac
    43021 ctgcaatcgg gtaacgatgc ttgtgtcgcc atggccgatt ttgccgctgg tagccaggac
    43081 gcttttgttg gcttgatgaa cagctacgtt aacgcactgg gcctgaaaaa tacccacttc
    43141 cagacggtac atggtctgga tgctgatggt cagtacagct ccgcgcgaga tatggcgctg
    43201 atcggccagg cattgatccg tgacgtaccg aatgaatact cgatctataa agaaaaagaa
    43261 tttacgttta acggtattcg ccagctgaac cgtaacggcc tgttatggga taacagcctg
    43321 aatgtcgacg gcatcaaaac cggacacact gacaaagcag gttacaatct tgttgcttct
    43381 gcgactgaag gccagatgcg cttgatttct gcggtgatgg gtggacgtac ttttaaaggc
    43441 cgtgaagccg aaagtaaaaa actgctaacc tggggcttcc gtttcttcga aaccgttaac
    43501 ccactgaaag taggtaaaga gttcgcctct gaaccggttt ggtttagtga ttctgatcgc
    43561 gcttcgttag gggttgataa agacgtgtac ctgaccattc cgcgtggtcg catgaaagat
    43621 ctgaaagcca gctatgtgct gaacagcagt gaattgcatg cgccgctgca aaagaatcag
    43681 gtcgtcggaa ctatcaactt ccagcttgat ggcaaaacga tcgagcaacg cccgctggtt
    43741 gtgttgcaag aaatcccgga aggtaacttc ttcggcaaaa tcattgatta cattaaatta
    43801 atgttccatc actggtttgg ttaaaaattg aacacttgaa agtgtaattt ccgtccccat
    43861 atactaagca tcagtaaaaa aactcccgcc ttctggcggg agttgctatt taattacgtt
    43921 acgccggagc tgacatgaaa accaaactta acgaactgct tgaattccct actcctttta
    43981 cttacaaagt tatggggcag gcgttacctg agctggttga tcaggtggtt gaagtggtac
    44041 agcgccatgc gccaggtgac tacaccccaa cggtaaaacc aagcagcaaa ggcaactacc
    44101 actcggtatc tatcactatc aacgccactc atatcgagca ggttgaaaca ctgtatgaag
    44161 aactgggcaa aatcgatatt gtccgcatgg ttctgtaact cgcttctccg ttacccggct
    44221 cctggtcggg taactcccct ctcctcgctg tgatatactt ttcccccact tttactcatt
    44281 ctccacggag atgccgtttt gtatcaggat aaaattcttg tccgccagct cggtcttcag
    44341 ccttacgagc caatctcccg ggctatgcat gaattcaccg atacccgcga taatagtacc
    44401 cttgatgaaa tctggctggt cgagcactat ccggtattca cccaaggtca ggcaggaaaa
    44461 gcggagcaca ttttaatgcc gggtgatatt ccggtgatcc agagcgatcg cggtgggcag
    44521 gtgacttatc acgggccggg acaacaggtg atgtatgtgt tgcttaacct gaaacgccgt
    44581 aaactcggtg tgcgtgaact ggtgaccttg cttgagcaaa cagtggtgaa taccctggct
    44641 gaactgggta tagaagcgca tcctcgggct gacgcgcctg gtgtctatgt cggggaaaag
    44701 aaaatttgct cactgggttt acgaattcga cgcggttgtt cattccacgg tctggcatta
    44761 aacgtcaata tggatctttc accattttta cgtattaatc cttgtgggta tgccggaatg
    44821 gaaatggcta aaatatcaca atggaaaccc gaagcgacga ctaataatat tgctccacgt
    44881 ttactggaaa atattttagc gctactaaac aatccggact tcgaatatat taccgcttaa
    44941 ttccatacat caatggccca atttacactg ggtcattact cataatacct gctaatgctt
    45001 tacatctcct tcgcgtttgt atattttctt tatcacttgc agaaatgcgg tgccacgcct
    45061 tgctccgtaa ctcaccccat tgattaacct a
//
LOCUS       DAZXUL010000021        44749 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZXUL010000021 DAZXUL010000000
VERSION     DAZXUL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44749
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.021"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(71..958)
                     /gene="rpnA"
                     /locus_tag="ACH3L1_001081"
     CDS             complement(71..958)
                     /gene="rpnA"
                     /locus_tag="ACH3L1_001081"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFT4075910.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
     gene            complement(1161..1397)
                     /gene="feoC"
                     /locus_tag="ACH3L1_001082"
     CDS             complement(1161..1397)
                     /gene="feoC"
                     /locus_tag="ACH3L1_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFT4075911.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1397..3718)
                     /gene="feoB"
                     /locus_tag="ACH3L1_001083"
     CDS             complement(1397..3718)
                     /gene="feoB"
                     /locus_tag="ACH3L1_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFT4075912.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3735..3962)
                     /gene="feoA"
                     /locus_tag="ACH3L1_001084"
     CDS             complement(3735..3962)
                     /gene="feoA"
                     /locus_tag="ACH3L1_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFT4075913.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4400..6721)
                     /locus_tag="ACH3L1_001085"
     CDS             complement(4400..6721)
                     /locus_tag="ACH3L1_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFT4075914.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6818..7294)
                     /gene="greB"
                     /locus_tag="ACH3L1_001086"
     CDS             complement(6818..7294)
                     /gene="greB"
                     /locus_tag="ACH3L1_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFT4075915.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            7522..8241
                     /gene="ompR"
                     /locus_tag="ACH3L1_001087"
     CDS             7522..8241
                     /gene="ompR"
                     /locus_tag="ACH3L1_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFT4075916.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            8238..9590
                     /gene="envZ"
                     /locus_tag="ACH3L1_001088"
     CDS             8238..9590
                     /gene="envZ"
                     /locus_tag="ACH3L1_001088"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFT4075917.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9666..11288)
                     /gene="pckA"
                     /locus_tag="ACH3L1_001089"
     CDS             complement(9666..11288)
                     /gene="pckA"
                     /locus_tag="ACH3L1_001089"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFT4075918.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11667..13418
                     /locus_tag="ACH3L1_001090"
     CDS             11667..13418
                     /locus_tag="ACH3L1_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFT4075919.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            complement(13562..14431)
                     /gene="hslO"
                     /locus_tag="ACH3L1_001091"
     CDS             complement(13562..14431)
                     /gene="hslO"
                     /locus_tag="ACH3L1_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFT4075920.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(14456..14857)
                     /gene="hslR"
                     /locus_tag="ACH3L1_001092"
     CDS             complement(14456..14857)
                     /gene="hslR"
                     /locus_tag="ACH3L1_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFT4075921.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14868..15536)
                     /gene="yrfG"
                     /locus_tag="ACH3L1_001093"
     CDS             complement(14868..15536)
                     /gene="yrfG"
                     /locus_tag="ACH3L1_001093"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFT4075922.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15601..17736)
                     /gene="igaA"
                     /locus_tag="ACH3L1_001094"
     CDS             complement(15601..17736)
                     /gene="igaA"
                     /locus_tag="ACH3L1_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFT4075923.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            18056..18616
                     /gene="nudE"
                     /locus_tag="ACH3L1_001095"
     CDS             18056..18616
                     /gene="nudE"
                     /locus_tag="ACH3L1_001095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFT4075924.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18781..21333)
                     /gene="mrcA"
                     /locus_tag="ACH3L1_001096"
     CDS             complement(18781..21333)
                     /gene="mrcA"
                     /locus_tag="ACH3L1_001096"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFT4075925.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21453..22232
                     /gene="hofM"
                     /locus_tag="ACH3L1_001097"
     CDS             21453..22232
                     /gene="hofM"
                     /locus_tag="ACH3L1_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFT4075926.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            22232..22771
                     /gene="hofN"
                     /locus_tag="ACH3L1_001098"
     CDS             22232..22771
                     /gene="hofN"
                     /locus_tag="ACH3L1_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFT4075927.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22755..23195
                     /gene="hofO"
                     /locus_tag="ACH3L1_001099"
     CDS             22755..23195
                     /gene="hofO"
                     /locus_tag="ACH3L1_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFT4075928.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            23185..23591
                     /gene="hofP"
                     /locus_tag="ACH3L1_001100"
                     /pseudo
     CDS             23185..23591
                     /gene="hofP"
                     /locus_tag="ACH3L1_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            23503..24741
                     /gene="hofQ"
                     /locus_tag="ACH3L1_001101"
     CDS             23503..24741
                     /gene="hofQ"
                     /locus_tag="ACH3L1_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFT4075929.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            25142..25663
                     /gene="aroK"
                     /locus_tag="ACH3L1_001102"
     CDS             25142..25663
                     /gene="aroK"
                     /locus_tag="ACH3L1_001102"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFT4075930.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25720..26808
                     /gene="aroB"
                     /locus_tag="ACH3L1_001103"
     CDS             25720..26808
                     /gene="aroB"
                     /locus_tag="ACH3L1_001103"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFT4075931.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26900..28192
                     /gene="damX"
                     /locus_tag="ACH3L1_001104"
     CDS             26900..28192
                     /gene="damX"
                     /locus_tag="ACH3L1_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFT4075932.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            28299..29135
                     /gene="dam"
                     /locus_tag="ACH3L1_001105"
     CDS             28299..29135
                     /gene="dam"
                     /locus_tag="ACH3L1_001105"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFT4075933.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            29153..29830
                     /gene="rpe"
                     /locus_tag="ACH3L1_001106"
     CDS             29153..29830
                     /gene="rpe"
                     /locus_tag="ACH3L1_001106"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFT4075934.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29823..30581
                     /gene="gph"
                     /locus_tag="ACH3L1_001107"
     CDS             29823..30581
                     /gene="gph"
                     /locus_tag="ACH3L1_001107"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFT4075935.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30574..31578
                     /gene="trpS"
                     /locus_tag="ACH3L1_001108"
     CDS             30574..31578
                     /gene="trpS"
                     /locus_tag="ACH3L1_001108"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFT4075936.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31848..32753
                     /gene="yhfZ"
                     /locus_tag="ACH3L1_001109"
     CDS             31848..32753
                     /gene="yhfZ"
                     /locus_tag="ACH3L1_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFT4075937.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32770..33132
                     /locus_tag="ACH3L1_001110"
     CDS             32770..33132
                     /locus_tag="ACH3L1_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFT4075938.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            33216..34379
                     /locus_tag="ACH3L1_001111"
     CDS             33216..34379
                     /locus_tag="ACH3L1_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFT4075939.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            34380..35606
                     /locus_tag="ACH3L1_001112"
     CDS             34380..35606
                     /locus_tag="ACH3L1_001112"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFT4075940.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            35603..36481
                     /locus_tag="ACH3L1_001113"
     CDS             35603..36481
                     /locus_tag="ACH3L1_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFT4075941.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            36492..36845
                     /locus_tag="ACH3L1_001114"
     CDS             36492..36845
                     /locus_tag="ACH3L1_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFT4075942.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36857..38161
                     /locus_tag="ACH3L1_001115"
     CDS             36857..38161
                     /locus_tag="ACH3L1_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFT4075943.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            38173..39258
                     /locus_tag="ACH3L1_001116"
     CDS             38173..39258
                     /locus_tag="ACH3L1_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFT4075944.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(39418..40149)
                     /gene="frlR"
                     /locus_tag="ACH3L1_001117"
                     /gene_synonym="yhfR"
     CDS             complement(39418..40149)
                     /gene="frlR"
                     /locus_tag="ACH3L1_001117"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFT4075945.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(40249..41034)
                     /gene="frlD"
                     /locus_tag="ACH3L1_001118"
     CDS             complement(40249..41034)
                     /gene="frlD"
                     /locus_tag="ACH3L1_001118"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFT4075946.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(41031..41861)
                     /gene="frlC"
                     /locus_tag="ACH3L1_001119"
     CDS             complement(41031..41861)
                     /gene="frlC"
                     /locus_tag="ACH3L1_001119"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFT4075947.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(41911..42933)
                     /gene="frlB"
                     /locus_tag="ACH3L1_001120"
     CDS             complement(41911..42933)
                     /gene="frlB"
                     /locus_tag="ACH3L1_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFT4075948.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLDELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42954..44291)
                     /gene="frlA"
                     /locus_tag="ACH3L1_001121"
                     /pseudo
     CDS             complement(42954..44291)
                     /gene="frlA"
                     /locus_tag="ACH3L1_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(44586..>44749)
                     /locus_tag="ACH3L1_001122"
     CDS             complement(44586..>44749)
                     /locus_tag="ACH3L1_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFT4075949.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHLAISISKI
                     IGGCGPTAQ"
BASE COUNT        10434 a        11352 c        12235 g        10728 t
ORIGIN      
        1 ctccaatgac tagtctaaaa actagtatta agactatcac ttatttaagt gatactggtt
       61 gtctggagat tcagggggcc agtctactcg ccgcagccaa ctcttcttct gacagcccgg
      121 taaatcgcat gatgtctgca agagggactc cggattcaag cattattttg gctatatgca
      181 gggctttgga ttgttccccc tcctgccgca atctttccgc aatagtcatt aaactctcct
      241 tgtgtttcgg tgaacgttcg gcaacgccgt cgataaaatc gttaaaacgt acagcgtcgc
      301 cagtttgcag tatgtaatta aacagccctt tgatttgtct gtcattagcg tatccactac
      361 ttaataagca ggccatttgc tctaccagcc ccatcagatc gcgttgacga atatgttttt
      421 gaattaactc cagcagcgcc atacgtcggt gctgcatgat ttcatcatca ggcatgacgg
      481 tgacatcaat cagcggaaat gcggaggcat aaagctgcct tgccagtttg ggatcggcga
      541 aacaatccag ccaacacagc gaatagggat aggggctttc aataccgtgg taaaacaaca
      601 atggcaccac catcggcaac gttttgtatc cagcatcaag gtgattttgc attgcggcaa
      661 tagcgtaacg catcatgcga aatgcgatca gtttgtttga ggtgctttga tgttcaatca
      721 gacaatagat gtatcctggt ccttgttccg ttttcaccga ccacagcaca tcggaatagc
      781 tttcacgcag atcgtcatca ataaagctcc tcgactccag cttgagggtt ttcatatcac
      841 agagcgcgtg aatgggtgcc ggtaaatgaa acgcaagaaa atcgcgtgcc gtctccggtt
      901 ggcgtaaaaa gagtttgaac agcgcatcat gtggggtgga actctgcttt ttactcatgg
      961 cgttccttca cctgttgttt gaatgccgcg actctacgcg ccactccaca tctgtgcatt
     1021 agcgagtttt catccctgcg agacgctttc agaggaactt acgttgcgct gcaagaattg
     1081 catttttatg gaaagccgca cgcaaaaaag cccggatgca aacgcatacc gggcttttca
     1141 aaacaggcga tggagtatgg ttaacgcagc gcccaccact cgcgcagaca ggctttgcct
     1201 tccgggcagc ttttacaact gccagagagg cagccgtcag gttcttcctg aatccgcacg
     1261 gctttgccca tgctttccag ctgttgcagc atggcgttaa tcattggctg cggagtgttc
     1321 aatgtctggc ttatctgggt cgcttccata cggccccgta acgccagcaa atcacgtacc
     1381 tgaataagtg aagccattaa tggcaatcac cggtggtgct ggctgcgcag caactgctta
     1441 ccgacttgcg ggttgccagc agttcgatat ccacccggct acgcgcgcgg cgcagcagac
     1501 cgatagctac gatgttaaac aggattaccg ccagaatgca caccaggctg taagttgggt
     1561 gctgactgta gctggcgacc tgatagaaca atgttgccag tgagtaagcg atattcagcc
     1621 cccacaggat ggagaagccc atccagccac ggcttgattc acgggcgatg gctcccatca
     1681 ccgagataca tggtacatac agcaggacga aaatcaggta gctgtatgcg gcagctgcgc
     1741 taccgaattt ctgatccatc acgcccatcg ccccggtacc catttcaccg ttgcctttgc
     1801 tagcttcaat ggggttcatc agtacgctaa ggctgaaggt gtctttcagg ctctgccagg
     1861 tttcatctac cgcactgaac agctcttcac cgaggttgaa ttccgccgga ttgaactctt
     1921 cgtcctgaat attttctgcg gtgtagaggg tgttgagcgt acccaccacc acttcttttg
     1981 ccattgcgcc cgtaaacagg ccaaccgttg cctgccagtt atcttcatgt acgccaatcg
     2041 gcttgaagac aggggttatc acccggctga cggaagccag cgccgagtcg ttgatgttat
     2101 cgacgatttt cccgctcaac gagaagctgt tgaaagcgct caggaaaatg ctgacgatga
     2161 taatcacttt accagcacgc agaacgaagc ccttcagacg ctgccaggtc tggataatca
     2221 ggcttttaac gtgtggtaca tggtagaccg gcagctccat gacaaacggc gtcgcttcac
     2281 cgcgcatgat ggtgtacttg agcatcaggc cagtcagcac cgccatcacg ataccgagca
     2341 tatacagcga gaaaaccgcc agggcaccgt tctgcccgaa gaaggcagcc gcgaatactg
     2401 cgaagatagc cagacgcgcg ccgcaggaca taaacggtgc catcatgatg gtcatcagac
     2461 gttcacgcgg tgcatcaagc gtacgtgcgc ccattaccga cggtacgtta caaccgaaac
     2521 cgacgatcag cggcacaaag gatttccccg gcaagcccag cgcctgcatc aggcggtcca
     2581 tcacaaacgc tgcacgcgcc atatagccgg agtcctcaag gaaggagagg aacaggtaca
     2641 tcatgccgat ctgcggcacc agtggcagca cggtgttaat accgccaccc aggccctggg
     2701 cgaggaagat agtcagccag tccgggaagt ggagcgtgta gccaatccat tgaataccat
     2761 gcacaaatag cgccacggag ccgacgttaa acagcggctg taacgccccg ccgatgttga
     2821 tagccagcag gaacatcagg tacatcacaa agaggaaaat cggcagaccg aggaaacggt
     2881 tgagcacgat tttatctacc gcagtggtga aacggctggg ttctgccgtc agggtgttgc
     2941 ttaccacatc acagatggca gcaatgcact ggtaacgcgc atcggcaatg tgcagcgccg
     3001 gatcatccat ttcattacgc agacgggcga gggtggcatc cagatgctgc gacgcttcac
     3061 cggcgtaggc gcggctgtag atatcgcctt ccagcatttg caggcccagc cagcgacgtt
     3121 gtttcagcgg gatgtcggaa ggcatcactt ttgccagtga atctgcttcg ttgagtagcg
     3181 gctgtgcgta atgcaccagt tccacattct cgttagcttt atagcgatca atcgccagct
     3241 tgagcgcttc aataccgcga ccacgggttg aaaccagcgg gatcaccggg cagccaagac
     3301 gtgccgatag agcatcaatt tcaatacgaa tattttgctt ctcggcaatg tcgagcatgt
     3361 tcagcgccac gatgcaggga atgccgagtt ccagcagttg cagcgtcagg tacaggttac
     3421 gctcaaggtt agacgcatcc accacgttaa tcagcaggtc ggcgtcgcca ctcaaaatgt
     3481 agtgacaggc gatttgctca tcgagcgagg tctgcgatga gatggtggtc agagaatagg
     3541 tgccgggcag gtccaccagc gtgacctgat gatcggtggt ggagaattgc ccttctttac
     3601 gttcgacggt aacgccagcc cagttaccta cacgctgacg tgcgccagtg agctggttaa
     3661 ataaggttgt cttgccagaa tttggattac caattaagcc aatggttaat tttttcattg
     3721 ttgttatcac cgtattaaca ggaaaccgct tccacttcta ataaggccag atcttttttg
     3781 cgtaatacca ggctcacacg acgggtttcg atatgaatgg ggtcgccgag tggagcgacg
     3841 cgcaccacat caaaagagga gccaggtaac atgcctagag aaagcagttt ttggcgatat
     3901 gccgggctga tttcacggga aaagccagtg attttccacg cagtatctgg agtgtattgc
     3961 ataggtgcct acttgtttct cattaactgg ataactacct catggggcgt ttgaccgcga
     4021 catgagacca gatgatttgc caacaaagga gagattaaat aatcaacaac acaatgataa
     4081 tgagaatggt ttctatcagc aatacttaaa atgtgtgcga atgttggttt gaataataat
     4141 ctgactcaat gttgatgtgg ctcaaggtta tatcgcagcg aaaatataat tcgaatagtg
     4201 agttacccgg actggctaat gtttaattaa ggtttcagtt gttaatgaag tgataaatat
     4261 taacggagta tattgtgagt ttggctgtgg aataaaagtg ccggagatat tctccggcac
     4321 ttttatgtca ggcaaagcgt tcgttgccag atgcggcgtg aacgccttat cgcgcaggtt
     4381 taggggccag tccgggcgtt taacgttttt tacccatcgc tgccgccagc gcatccatca
     4441 tcgcgctatt accggcaggc tgcgcttcac gaccgcgtgg tttggctgcc gggcggttgt
     4501 tttgcgggcg ttcattaccg ccgccgcgac gagagttggt ttcgccaggc tgctcgtcca
     4561 gacgcatagt cagggcgata cgtttacgct gaagatccac ttccagcact ttcaccttca
     4621 caatgtcgcc cgctttcacc acggtatgcg gatcttccac aaacttgttc gacaatgaag
     4681 agatgtgaac caggccgtcc tgatgcacgc caatatcgac aaacgcgcca aagttggtga
     4741 cgttggtcac tgcgccttcg aggatcatac ccggttgcag gtcgttcatt gtctcgacgc
     4801 catcggcaaa ctgagcggtt ttaaattccg gacgcggatc gcgacccggt ttttccagct
     4861 ctttgatgat gtcagttact gtcggcacac cgaatttttc atcagtaaag tcagacgctt
     4921 tcaggttacg cagttcgctg ctgttaccca tcagatcttt cagtgcctgc tgtgttgctg
     4981 ccagaatgcg ttccaccacc ggataggctt ccggatgaac ggtagaggcg tccagcgggt
     5041 tatcaccgtg gttaatgcgc aggaagcccg cgcactgctc gaaggctttc ggccccagac
     5101 ggctgacttt cagcagttgc tgacggttct ggaactggcc gttctcatcg cgccaggcca
     5161 cgatgttttg cgccatcatg cgcgtcaggc ccgccacgcg ggttaatagc ggaacagaag
     5221 cggtgttgag atcgacgcca acggcgttta cgcagtcttc tactactgca tccagtttgc
     5281 gggccagttg cgtctggctg acgtcatgct gatactgacc tacgccgata gatttcggat
     5341 cgattttcac cagctccgcc agcggatcct gcaaacggcg ggcgatagat accgcaccac
     5401 gcagcgaaac gtcgagatcc gggaactcct gcgctgccag ctcggaagct gagtaaaccg
     5461 acgcgccagc ttcgctgacg atcactttct gtgcggtcac tttcgggaac tgcttctgca
     5521 cgtcgagata gaaacgttca gtttcgcggg aagctgtacc gttgccgatc gccaccagtt
     5581 caacgttatg tttttcacac aaggcagcaa cggtcatcgc tgcttttgcg gcttgtccgg
     5641 tgtgcgggta aatggtatcg gtcgccacca gtttgccagt ggcatcgacc accgccactt
     5701 ttaccccggt acgcagaccc ggatcgaggc ccatcgttgc acgcagtccg gcaggggccg
     5761 ccatcagcag atcgtgcagg ttacgggcaa agacgttgat tgcttcatct tccgcgcgtt
     5821 cgcgcacggt acccatcagt tcggtttcca gatgcatcag caccttgatg cgccaggtcc
     5881 agctcactac gcttttgcgc cagctatccg ccggggcatt gttcaggcgc aggccaaggt
     5941 gatccatgat gatttgctcg caatagctct ctttgggcgg ctcatcgaac tgcggatcgg
     6001 cattcagcga aagctggagt acgccttcgt tacgcccacg gaacatcgcc agcgcgcggt
     6061 gagaaggcac cgtggacaac ggttcgtgat gatcgaaata gtcgcggaat ttcgcccctt
     6121 cctcttcttt accgctcacc accgtagaaa ccaaatgcgc gttcttccac agataatcac
     6181 gcactttcgc cagcagcgtg gcatcttcgg caaaccgttc catcaggata tagcgcgcgc
     6241 catccagcgc ggctttggta tctgccacgc ctttatcggc atcaacatat tgtgcagcgg
     6301 cgacttctgg cgtgtgtgac ggatcgctcc acagcaggtc agccaacggc tcaagccctg
     6361 cttcaatggc gatttgcccg cgggtgcggc gtttaggttt gtagggcagg tagaggtctt
     6421 cgagttcggt tttgcttagg gtggcgttga tggccttcgc cagatcatcg gtgagtttgc
     6481 cttgctcgga aatggatttg aggatcgcct gacgtctctc ttccagctcg cgcagatagc
     6541 tcagacgcgt ttccagatta cgcagctgcg tgtcatccag accgccggtg atttccttac
     6601 gataacgtgc gataaacggc acggtattcc cttcgtcaag caggcgaacg gcagcgtcaa
     6661 cctgttccgg gcgcgcctga atttcacccg caataatgcg gcagaacgaa tcattcatca
     6721 tggtttgagt tcatcttttc ggatcaaaaa tcaggggata gttatacgga ctggctggca
     6781 aaaatgccag ccatcggcag gaggttaaga ctcttcctta cggtttcacg tactcgatag
     6841 cattaacata ccagttcgct tccccggcag gggtattcac caccgccaga tcgccgactt
     6901 cttttttcag caatgcgcgg gccatcgggg aatcgataga gatgtaatct ttacggccaa
     6961 aaatttcatc gtagccgaca atacggaaac ggtgagtcac gccatcgtcg ttttcaatct
     7021 ccacccacgc gccaaaaaag actttgcctt cctgctgagg ggagtaatcg acgattttga
     7081 gattttccag gcatttagtg agatagcgca cgcgacggtc gatttcacgc agacgctttt
     7141 tattatactg atagtcagca ttttcgctgc ggtcgcccag acttgcggcc caggtcacct
     7201 tttttgtgac ctccgggcgt tcttcacgcc agagataatt aagctcttgt ttgagttttt
     7261 catacccttc ccgggtaacc aggggcgttt tcatctcgtt gattcccttt gtctgtttga
     7321 taatgcgcac attgggtata acgtgatcat atcaacagaa tcaataatgt ttcgccgaat
     7381 aaattgtata cttaagctgc tgtttaatat gctttgtaac aatttaggct gaaattcata
     7441 ccagatttag ctggtgacga acgtgagctt ttttaagaat acacgcttac aaattgttgc
     7501 gaacctttgg gagtacaaac aatgcaagag aactacaaga ttctggtggt cgatgacgac
     7561 atgcgcctgc gtgcgctgct ggaacgttat ctcaccgaac aaggcttcca ggttcgaagc
     7621 gtcgctaatg cagaacagat ggatcgcctg ctgactcgtg aatctttcca tcttatggta
     7681 ctggatttaa tgttacctgg tgaagatggc ttgtcgattt gccgacgtct tcgtagtcag
     7741 agcaacccga tgccgatcat tatggtgacg gcgaaagggg aagaagtgga ccgtatcgta
     7801 ggcctggaga ttggcgctga cgactacatt ccaaaaccgt ttaacccgcg tgaactgctg
     7861 gcccgtattc gtgcggtgct gcgtcgtcag gcgaacgaac tgccaggcgc accgtcacag
     7921 gaagaggcgg taattgcttt cggtaagttc aaacttaacc tcggtacgcg cgaaatgttc
     7981 cgcgaagatg agccgatgcc gctcaccagc ggtgagtttg cggtactgaa ggcactggtc
     8041 agccatccgc gtgagccgct ctcccgcgat aagctgatga accttgcccg tggtcgtgaa
     8101 tattccgcaa tggaacgctc catcgacgtg cagatttcgc gtctgcgccg catggtagaa
     8161 gaagatccag cgcatccgcg ttacattcag accgtttggg gcctgggcta cgtctttgta
     8221 ccggacggct ctaaagcatg aggcgattgc gcttctcgcc acgaagttca tttgcccgta
     8281 cgttattgct catcgtcacc ttgctgttcg ccagcctggt gacgacttat ctggtggtgc
     8341 tgaacttcgc gattttgccg agcctccagc agtttaataa agtcctcgcg tacgaagtgc
     8401 gtatgttgat gaccgacaaa ctgcaactgg aggacggcac gcagttggtt gtgcctcccg
     8461 ctttccgtcg ggagatctac cgtgagctgg ggatctctct ctactccaac gaggctgccg
     8521 aagaggcagg tctgcgttgg gcgcaacact atgaattctt aagccatcag atggcacagc
     8581 aactgggcgg cccgacggaa gtgcgcgttg aggtcaacaa aagttcgcct gtcgtctggc
     8641 tgaaaacctg gctgtcgccc aatatctggg tacgcgtgcc gctgaccgaa attcatcagg
     8701 gcgatttctc tccgctgttc cgctatacgc tggcgattat gctattggcg ataggcgggg
     8761 cgtggctgtt tattcgtatc cagaaccgac cgttggtcga tctcgaacac gcagccttgc
     8821 aggttggtaa agggattatt ccgccgccgc tgcgtgaata tggcgcttcg gaggtgcgtt
     8881 ccgttacccg tgcctttaac catatggcgg ctggtgttaa gcaactggcg gatgaccgca
     8941 cgctgctgat ggcgggggta agtcacgact tgcgcacgcc gctgacgcgt attcgcctgg
     9001 cgactgagat gatgagcgag caggatggct atctggcaga atcgatcaat aaagatatcg
     9061 aagagtgcaa cgccatcatt gagcagttta tcgactacct gcgcaccggg caggagatgc
     9121 cgatggaaat ggcggatctt aatgcagtgc tcggtgaggt gattgctgcc gaaagtggct
     9181 atgagcggga aattgaaacc gcgctttacc ccggcagcat tgaagtgaaa atgcacccgc
     9241 tgtcgatcaa acgcgcggtg gcgaatatgg tggtcaacgc cgcccgttat ggcaatggct
     9301 ggatcaaagt cagcagcgga acggagccga atcgcgcctg gttccaggtg gaagatgacg
     9361 gtccgggaat tgcgccggaa caacgtaagc acctgttcca gccgtttgtc cgcggcgaca
     9421 gtgcgcgcac cattagcggc acgggattag ggctggcaat tgtgcagcgt atcgtggata
     9481 accataacgg gatgctggag cttggcacca gcgagcgggg cgggctttcc attcgcgcct
     9541 ggctgccagt gccggtaacg cgggcgcagg gcacgacaaa agaagggtaa ataaacggga
     9601 ggcgaaggtg cctcccgttt tgctttctat aagatactgg atagatattc tccagcttca
     9661 aatcattaca gtttcggacc agccgctacc agcgcggcac ccgcaggggt gtcggtgtat
     9721 ttatcgaagt tgtcgataaa cagtttcgcc agggtttcgg ctttttcctg ccactgttcc
     9781 ggagaagcgt aggtgttacg cggatcgaga atcttcgtgt ctacgcccgg cagttcggtt
     9841 gggatcgcca ggttaaacat cggcagagtg aaggtttctg cattatccag cgaaccgttg
     9901 aggatggcgt cgataatggc gcgggtatct ttaatcgaga tacgtttgcc agtgccgttc
     9961 cagccagtgt taaccagata agcctgcgcg cccgccgcct gcatacgttt caccagcact
    10021 tctgcgtact gagtcgggtg cagcgacagg aacgccgcgc cgaagcaagc ggagaaggtt
    10081 ggcgtcggtt cggtgatgcc acgctcagta ccggccagtt tggcggtgaa gccagagagg
    10141 aagtgatact gggtttgatc ggcagtcagg cgagaaaccg gcggcaacac gccgaaagca
    10201 tcagcagtca ggaagataac cttagtcgcg tggcccgctt tggaaaccgg cttaacgatg
    10261 ttatcgatgt gatagattgg ataagaaacg cgggtgttct cggtttttga accatcatca
    10321 aagtcgatag tgccatcttc acgcacggtg acgttttcca gcagcgcatc acgacggata
    10381 gcgttgtaga tttcaggttc cgcttctttc gacagcttga tagtttttgc gtagcagccg
    10441 ccttcgaaat taaacacgcc gtcatcgtcc cagccgtgtt cgtcatcgcc aatcaggcgg
    10501 cgtttcgggt cggtggaaag ggtggtttta ccggtgccgg aaaggccgaa gaacaccgca
    10561 acatcgcctt tctcgccaac gttggcggag cagtgcatag aagcgatacc tttcagcggc
    10621 agcaggtagt tcatcatcga gaacatccct ttcttcattt cgccgccgta ccaggtgccg
    10681 ccaatcagct gcatgcgctc ggtcaggtta aacgccacga agttttcgga gttcagaccc
    10741 tgttctttcc actgcgggtt agtgcacttc gcgccgttca taacgataaa gtctggtttg
    10801 aaacctgcca gctcttcatc gctcgggcga ataaacatgt ttttgacaaa atgcgcctgc
    10861 caggccactt cggtgatgaa acggacggaa agacgagtat ccgggttcgc accacagaaa
    10921 gcgtcgacaa cgaacagacg tttgccggaa agctgcctgg tcaccaggcc tttcagatgc
    10981 tgccaggttt ccggagagag aggtttgttg tcgttcttac ctttgccttt gtctgcccac
    11041 cagaaagtat cgcgagtggt atcgtcacgg acgatatact tatcttttgg tgaacgaccg
    11101 gtgaagatcc cggtatcgac ggcaacggca cccagattag ttaacacccc gcgctcataa
    11161 cctgtcaggc tcggatcgag ctcttcctga tacagcaggt cgtagcttgg gttgtaaacg
    11221 atatcatgta cgtcactgat accataagcc tcgagttctt gcgggggcaa accattgtta
    11281 acgcgcattt cactgctcct tagccaatat gtattgcctg aatagtaaag tctttttggg
    11341 ggtgttaacc gcgacaaggc tcatagattt acgtatctgg agaaattcat atttgacaaa
    11401 aaactccgtg attcctgtca cgaaacggtt gctattatcg cagaaatagc attttaggtg
    11461 tggaaaatgt tctcaaaagg ttaaatcttg gtttatatgg cgggaatatt gtgagtggaa
    11521 tcgcattcct gaatgaaatt gatgcaaatt caaccggtaa agtatcgatt caccacgcta
    11581 actttttttg attattttct gaaaaaagca aaatactact gcccggatgc gataattaac
    11641 ccaccttttg attctggata accaacatgg ataacgttga actttcaccg gcaacacgct
    11701 gggggatgat tgctaccgga ttacttcagg ggatgattgc taccggatta cttcaggggc
    11761 tggtttgcta cctgcttatc gcctggcttg ccggaaaaaa tcacagttgg attgtttatg
    11821 gcgtgcctgc gaccgtcgcg ttttcatccg ttttactctt ctccgtgatc tcttttaaac
    11881 agaagcgcct ttggggatgg ctggcgctgg tgtttattgc cacgacaggg atgagcggtt
    11941 ggctgaagtg gcaaactgat ggcatgaacc cctggagagc tgaaaaggcg atttgggatt
    12001 ttggctgcta tctgctgttg atggcaatgt tgttgctacc gtggatacaa caaagccttc
    12061 gtatccgcaa tggcagtagc cgctatagct acttttacca gtcagtatgg cataacgtac
    12121 ttatattact ggtgattttt ctctccaatg gcctgacgtg gctggtgctt ttactgtggg
    12181 gtgagttgtt taaacttgtt ggcatcacgt ttttcaatac gctctttttt gcaaccgact
    12241 ggtttatgta tcttacattt ggtctggtta ccgcactggc ggtgatcctc gcgcgtacac
    12301 agtcacgttt aatcgactct attcaaaagt tgttcacgct aatcgccacg gggttgctgc
    12361 cgttagtatc attgctaacc ctgatgttta tcatcaccct gccgtttacg ggcctgagcg
    12421 cgatttctcg ccacatctcc gccgccgggt tgctgttaac gctggccttt ttgcaattga
    12481 tcttaatggc tattgtccgc gatccgcaaa aagcgtcact tccctggacg gggccgttgc
    12541 gttgcctgat taaaaccgct ttgctggttg ctccgctgta tgtgttcgtc gccacctggg
    12601 ggttatggct gcgggtcgct cagtacggct ggactgtcga ccgcttgcag ggcgcgctgg
    12661 cggagctggt gttactggtg tggtcgctgg ggtattttgt cagtatcgtc tggcgtaatg
    12721 ggcaaaatcc ccttgttctt cagggcaaag tgaaccttgc ggtttcgtta ttggtgttgg
    12781 tgatactggt gcttcttaat tcgccggtgc tggacagtat gcgtattagc gtgaacagtc
    12841 atatggcgcg ttatcagagc ggcaaaaaca cgccagacca ggtaactatc tacatgctcg
    12901 aacagagcgg tcgctatgga cgtgcggcgc ttgagtcgct gaaaagcgat gccgggttta
    12961 tgaaagaccc gaaacgcgcg cgggatctgc tgatggcgtt agatggagag caacatcttc
    13021 aggaacaggt atcggaaaaa gtattcgccg aaaatgtgtt aattgcccct ggttctgtta
    13081 aaccggatgc gacattctgg tcggccttaa tccaggatcg ctataacgtg atgacctgta
    13141 ttgaaaaaga cgcctgcgta ctggtcgagc aagatctgaa tagtgatggt caggcggagc
    13201 ggatcctgtt tgcttttaat gatgacagag tcattgtcta tggctttgac tcagacagaa
    13261 aagaatggga cgcgcttgat atgagtttac ttccgaacga aataacgaaa gaaaaattac
    13321 tcacagctgc gaaggatgga aaactgggga cgaggccgaa agcgtggcgc gatctgacgg
    13381 tagatggtga aacgctggaa ataaacttaa gtaagtgaca acgcttttat cggatacgac
    13441 gctgtatctg accaaaataa tttgcgattt atttcatagg cgctggtaag cgcagcacat
    13501 caggccatta agtcgtaaaa tccggcagag ccctctctgc cggacatact cattaatgaa
    13561 cttacggatc tgccggagac gcgttgttgc ggatttcagc aatatccatc gcattgaaca
    13621 gatagtggtt accgcagtaa tcacaatgca tgtcaatttc gccatcttcc gccaggatgc
    13681 tatcaacttc ttcatcaggc agcgttttca gcgcatcggc gcaacgttca cgcgagcagg
    13741 tgcatttgaa ctccacatcc tgcggatcgt aaaccgtcac ctcttcttcg tgatacaaac
    13801 gccacaacac ttcgtttgcc ggtaaggtca gcagttcttc ggttttgatg gtttcggtta
    13861 gcgtcgccag gtggtcaaag tcgtcctgtt gggtattttg cgcaggcatt acctgcaaca
    13921 acataccgcc tgcagccggt ttgccgtcta cgtcgccggt gcgaataaac aggcgcgtcg
    13981 gcagctgttc agaacgcata aagtaatctt ctaggcaggc cgccagggta tcaccttcca
    14041 gaccaactac gccctgatag cgttcgcctt cgctcggggt aatggtgatc accacgtaac
    14101 cattgccgac cagcgttttc aggtcggcat tttctggaat ttcgccctgc acgcgcgcca
    14161 caccgcgcat ttgctggtta ttgttaccgt taataaccgc cagattcatc ggaccgtcgc
    14221 cctgcagctg tacggtgata tcaccatcaa acttcagcgt agcggttaac aggctggtcg
    14281 caaccagcag ttctgccagc acgtttttaa cgggctgcgg ataatcgtgg ttctcaagga
    14341 tctgttgcag ggtttccgaa acggttacca gttcgccgcg aacggcaaaa ttttcaaaca
    14401 gatagcgatg taattggtca tgttgcggca taatcatctc tcttgcaggt gacagttatt
    14461 cactgtcgcc gtgtttaaat cgtaacaggt cgcggcgctc ttttttgtcc gggcgtcggt
    14521 ccgggtgcgg catggttaag gcattaagtt tacgtgccat cgccattttt tcgcgtttct
    14581 ctacactttc cgcagtctct tcatacagca aggctgcctc gctggcgggg cgacgctgtt
    14641 cagtaatcgc ctttacaatc accgtgcgtt cgtcatttcc ctggcgcaga gtgagcgtgg
    14701 cattcagctc gacgattttg ctcggcttgc tgcgctgccc gttgtaatgc accttaccgc
    14761 cttcaatcat ttcacgggcc agcgcgcggg ttttataaaa acgggcagcc catagccatt
    14821 tatccagtcg aacctcaaca gcaggtttct ctttcatggc gtctccttca cattagcgag
    14881 gggatcaggc ggcggtagtc attcagtgac ggatggcgtt gatactgttt ctcggcaatc
    14941 ccggaatcag gattagtcac gccgaggcag taacgaatac caaattgcgc ggcagcatcg
    15001 agaatcgctt cgctgtcatc aataaacagc gttctttcag ctttcagacc cgtagcttcg
    15061 gccaccgcat gccataaccg ctgatcctct ttcggataac caaatgtgtg ggtggaaagt
    15121 aataaatcaa ggtgtgcgtc cagacccgta tgctcaagtt ttaccgccag gttgtgcgga
    15181 tgagcattgg tgagcaaaat tcgctgctta ccgctggctt tcagtgcctc aagaaacgga
    15241 atggtatctt cacgcagtac ggcgcgcggt cccatctcgg tggtcatcgc acagatatcc
    15301 agacccagtt gctcactcca gtaatcaaga cagtaccagt ttagcgtatg ctgtacgtcg
    15361 tgatattgct ggcgcatata ttccatcgct tcctgtggcg taaccccgtt tttcgcgccc
    15421 catgtttcag gcaccagctt ttgccagaaa tagttatcga aggcgaggtc gagcaacgtg
    15481 ccgtccatat ccagcagaac ggtatctacg tcctgccagg caatgttgat atgcatgagg
    15541 gaaatctcca gagtgaagca atttgcgcga cagggtagca taacctgccg cgcaaacgtg
    15601 ttattcgata aggctttctg aaggggtgat cagttgcggg ttcaggcagc tttcataata
    15661 ggcctgaatc tccatcatgc gagtgcgatg acgctggtaa cgacgccagg cctgtacgcc
    15721 attgtaaatg gcgctaccca gcatcgtcag tagcagcaat gtggtgctga gatagcgcca
    15781 caggccggaa cgatccggga tcggatgaag gccaatatgc tgcgtaccat tggcgtcagt
    15841 gaagattttt gtgacgatac cttcggcgtt aaacggcgta tgcatcagca tttgtgccag
    15901 tttctggaaa gcgttccact gttcttgcgg cgggtagtcg taaagtgatg ccgaaggcca
    15961 gggctgatca acaaaatcgc tgccttcgtc gctgacaatc aggaatccgc cgggggccgg
    16021 actgtttagt gattgtgccg ctcgcgccgt ttcatgcgtg ataaacggcg cggtggaggt
    16081 tgccaccagg ttatccagcg attcagcact caccgggcgt aataacacat tcacgccatc
    16141 gagcttcccg gcgttggcgc gttttaccag cgcgtcccag tctttactgt tgccgagatt
    16201 gaccagcgca tttttcagtc gcacacagtc atctttggca gaacataaat ccgctgtctt
    16261 cagtacaatg tcgccaaaat catcaagcaa taccatgccg gatttttgaa tcgctgaacg
    16321 taatgaggca ctgacgcgag attcatcttc cggtttaggg tgcagctggc gattaactgc
    16381 ttcagtcaat gccgtcgctt tgttgaccag ttcagattct ggtaatggta atgagcgggc
    16441 gtcattccag atgatctgcg agcagtcaaa cggtaaaaaa ggtgaattgg ttttcgcgct
    16501 ccaggttccg gaagtgcgaa tattacacat tcccgtaccg ctaatacgca atgtatcgcc
    16561 tacccgcacg ccagcgtcag ccagttgttt tacgctggtg gcttcaatgg tctgcgcgcc
    16621 tttcatccat gagagagtga atttcagcgg catatccagc gggatccaga ataacagcat
    16681 aaacagcact agcagcgagc ccgcagcgat aatcgtactg cgcagccagt gctgtaacgg
    16741 aaagtttttt acttcatcat gcagcgaaag atatcgcccc tgacgcacta cgtggcggtc
    16801 gagatagata tcaatatcgg tttgttgacc gagatcctga gcaatgtatg gctgccagtg
    16861 tgcgggatag accaggtcga taataccgag tgaaatattg ttgatctgtt cctgatcgtt
    16921 ttcgccaaac aatccccaac gacggggtgt accgcgcaga caatgaattt ctcgcaggga
    16981 ggattttgcc gggggcgcga atagccccca cagacctgcg cccagcagca gtaatgcgcc
    17041 gcctgccagc cacggaacaa atacatccgg ggtaatcagg cagaaaaaga acatcaggaa
    17101 ggaggcgacg atcagcaacg cttcacgcag cccgcgcgga cgactcagcg catattcttc
    17161 atgcgtttct ttgcgaatat tgagtagctc gatttgctca ctctcttccc cgcgaatcga
    17221 cgcctgcgtg gacggaacag gttgcaaggc atagccgcgc gtttcctgca tgtactcctg
    17281 cagcgtatga ccgttgagtg aaataaccaa cggcagcgaa tcggtatgaa tcagttcaac
    17341 ggtattctca tcgttgatgt actgttccca gaaggggggc aggtggactt ctaccgaatc
    17401 gaggtagtaa cgccatttat tcggatcgtc ggtagagatg ccgtaacgtg tgatagcgtg
    17461 tgtcagcatc atgacgttgt tgctttcggc attcagcgcc agagagatcg gtgccgcgct
    17521 agcttccgtt gggccaggca cctgtaatac ctgcgtcagg ctctcaagat aattttcaac
    17581 ggcgctacgt tcttcaggtg tgagtttacg cgtttgcgca tccgcgaagg cgttggtcca
    17641 gggcagctga cgccgtctgg atcgcacttt tatcagccat cccgcaagta gtgagcaggc
    17701 cagcaaagca gctaaaaaaa tcacaatggt gctcatgctt tccccatctt acttagtctg
    17761 tcaggcgtgg tcagtcgcac cggacacaat acatgattct gcggttggct atcggcaagc
    17821 gtggagctta acttgagcat cattaagcac atcacagtaa ccgctgatca tagcaaaccc
    17881 tctgaaggcg gaatatcagc aaattcctgg agttatttca acctattgac ttttaatttg
    17941 atttgaggac gattatttat taagttacat aaatgtaaat aacatgcaat tcacattgcc
    18001 gaaagcgtgt ccgatatcgc acaataaccg ctgatttcac agcatagatc ccacgatgaa
    18061 caaatcatta caaaaaccca ccattctgaa tgttgaaact gtagcccgtt cccgactgtt
    18121 taccgtcgag agcgtggatc tggagttcag caatggcgtg cggcgtgttt atgaacgaat
    18181 gcgtccaacc aaccgggaag cagtgatgat tgtgccgatt gtggacgatc acctgatcct
    18241 gatccgcgaa tacgcagtgg gaactgaatc ctacgaatta ggtttttcga aaggattaat
    18301 tgatccgggt gaaagcgtct acgaagccgc taaccgcgag ctaaaagaag aggttggatt
    18361 tggtgcgaac gatctgactt ttttgaagaa gctcagcatg gcaccgtctt acttttccag
    18421 caaaatgaat atcgtggtcg cgcaagatct ctacccggaa tcgctggaag gcgatgagcc
    18481 agagccgcta ccacaggtgc gctggccgct ggcgcatatg atggatttgc tggaagaccc
    18541 tgacttcaac gaagcgcgta atgtcagcgc gctgttcctc gtgcgcgaat ggttgaaagg
    18601 gcaggggcga gtgtaaatgc gtgaagtttg cctgatgcac aacgcttatc aggcttatgg
    18661 ggttcctgca atatattgat ttttcacgat tttgttagcc ggataacgcg ttcacccgca
    18721 tccggcataa acaaatgcac tttgtcagcc aactgaaaag gcgccgaagc gcctttttaa
    18781 tcagaacaat tcctgtgcct caccattatc gataatggtc gttcccacct cgtgcaccgc
    18841 ctgttgtgtc ggctgcgtac cttcgatgaa atactcttcg cggctgttgc caccattagc
    18901 taactgcccg gtgctgcgat cgatattcac cgtcacaata cccggaggcg gcgtcagcgg
    18961 ctgctccggc acaccttcaa gaacggcttt catataagcg tcccatgcag gctgggcact
    19021 cttggcaccg ccttcgtaac ctgagatctg atctttaatc gctccggaag ccgttgtatg
    19081 accgagatta cgacggtgat catcaaagcc aatccagacc gaggtcacaa cgcccggacc
    19141 gtaacccgag aaccacgcat ctttcgaact gttagtggta ccggttttcc cgccgatatc
    19201 gcgacgctgc aaatcacgac ctgcacgcca gccggtaccc tgccagcctg gttcaccaaa
    19261 gatattggtg ttcaaagcac tcttaatcag gaatgccagc ggagtgttga tgacgtgcgg
    19321 tgcgtactcc tgcgcgccag tcttcgccac taacgcctga tttgcctgct ccagctgcgg
    19381 cattggtaca gaaacattct gctgctcgcg ggagatagcg acatcttcaa catcgttatt
    19441 ttccagcacg ttcgatttct gcgtatcacc gtaaatcact ggaatatcgc attccgggca
    19501 ggctactttc ggtttcgctt cgaaaatcac gccgccctga tcgttttcaa ttttgctgat
    19561 aaaccacggg tccactagga agccgccgtt cgccatgacc gcgtagccgc gcgccacctg
    19621 cattggggtg aaggacgctg aacccagcgc cagcgattcg gtgtggacaa tgttttgcgc
    19681 cgggaagccg aagcgttgca gatattctgc agcgtagtcg acgcccatcg cccgcattgc
    19741 acgtaccatc accacgtttt tcgactgacc cagcccctga cgtaagcgaa ttggaccagc
    19801 atactgcggt ggtgagttct tcggctgcca gtcagaaccg gcacctgcat cccagcgaga
    19861 aattggcaca tcgttcaaca tacttgccag cgtcagacct ttatccatcg ccgcggtgta
    19921 gaggaacggt ttgatgttgg aacccacctg acgcagtgcc tgggtggcgc ggttaaactt
    19981 gctctgattg aaatcaaagc caccgaccag cgccataacg gcaccatttt gcggattgat
    20041 cgacaccagc gccgagttca cttccggcac ttgtgccagc caccatgcat cgccaacctg
    20101 acgaacccag atttgctgac ccgtttgcag aacatcggtc actttacgcg gcgtcggtcc
    20161 ctgctgagta tccgaacggt aaggacgcgc ccagcgaacg ccttccatac tcaatgcgac
    20221 ggtcgacccg tccgccagca tcgccgtcgc tccctgagga ttggcgctgg tgactgcggc
    20281 aggcagcagc ggaccatagg tcggcagcgc cttcagcgta tcggtaatct tgttgttatc
    20341 ccacgccgac tcgcccactt tccacagcac atttgccggg ccgcgatagc cgtggcgcat
    20401 gtcgtagtcc agcacgttat tacgtaccgc ctgctgcgcg gcctgctgca ctttgcgggt
    20461 gatggtggtg taaatgcgat aaccgtcttc ataggcactt tcgccataac ggttatacat
    20521 ctcctggcgc accatttcgc tcaggtacgg cgcagagaaa gcaatctccg gcgcgtgata
    20581 attagcgtta atcgcctcag tgcgtgtctg atcgaactgt tgctgggtga tatacccttc
    20641 atccagcatc cgcgacagta cgacgttacg ccgcgcgacg gcacgatcca ttgagtagag
    20701 cggattaaag gtggaaggcg ctttcggcaa cccggctatc accgccattt cgttcagcgt
    20761 cagttggtcg accgttttac cgaaatagac ttgtgccgca gcaccgacac cataggcgcg
    20821 gtaaccaagg taaatcttgt tcagataaag ctcgaggatt tcgtctttcg tcagcaactg
    20881 ttcaatgcga atcgcgagga agacttcctt aatcttacgc atcatcgtgc gttctggact
    20941 gaggaagaaa tttctcgcca gctgctgggt aatggtactc gccccttgtg acgcgtggcc
    21001 ggagaacagc gccacacttg ccgcacggaa gatccccacc gggtcaacgc cgtgatgctc
    21061 gtagaagcgg ctgtcttccg tcgcgataaa ggctttcacc atctccggtg ggatttgatc
    21121 caacgtaacc ggaatacgac gtttctcacc gtattgagca atcagctcgc catcggcgct
    21181 gtaaatctgc atcggaattt gcaggcgaac atcttttaat gtcaccacat ccggcagttg
    21241 tggctcgatg tagcggtata ggccataaat cgagcctgct cccagcagaa tgcaacagac
    21301 tgcaaggatc aaaaaatact ttacgaactt cactggaaat ttcccattta gtttcatttg
    21361 ggcagtttat aaacaaacgc gcggtagtat aaaggcaagc cagacgcatt gatatacccg
    21421 tcagagtgac gggtgataag gagatcatca caatgacatt taagatctgg caaattggtt
    21481 tgcatttaca acagcaagaa gcggcagcgg ttgcgatcgt gcggggcgca aaagaatgct
    21541 ttttgcaacg ctggtggcgg ttgccgctgg cgcacgacat tatcaaagat gggcggattg
    21601 ttgatgcgca gcagctggct aaaacgttgt taccgtggag tcgcgaactg ccgcagcgtc
    21661 atcacattat gttggcgttt cccgccagtc gcacattaca gcggtcattt ccgcgcccgt
    21721 cgatgtccct tggtgagcgg gagcaaacgg cctggctgtc agggacgatg gcccgcgagc
    21781 tggatatgga tccggactcc ctgcgcttcg attacagcga agactcactc agccccgctt
    21841 ataacgtgac tgccgcgcaa agcaaagagc tggcaacgct gcttacgctg gcagaaaggt
    21901 tgcgtgttca tgttagtgcg atcaccccgg atgccagtgc attacagcga ttcctgcctt
    21961 ttttaccttc tcatcagcaa tgtctggcct ggcgtgataa cgaacagtgg ctgtgggcga
    22021 cgcgctatag ctgggggcgc aaactggcgg tggggatgac tagcgcgaag gagctggcgg
    22081 cagcgttatc cgttgatccc gacagcgtcg cgatatgtgg cgaaggcgga tttgacccct
    22141 gggaggccgt ttctgttcgt cagccgccgc taccgccgcc tggtggagac tttgccatcg
    22201 cgctggggct ggcgcttggg aaggcgtact gatgaatccg ccaattaatt ttttgccctg
    22261 gcgacagcaa cgccggaccg cttttctgcg tttctggttg ctgatgttcg ttgcgcctct
    22321 gctgctggcc gtcgggataa cgctaatact gcgtctgaca ggcagcgccg aagcgcgcat
    22381 agacgcagtt ttgcttcagg cggaacaaca actcgcccgc agcttacaga taacgaagcc
    22441 acgtttgctg gagcaacagc agttacgcga acagcgttcg caacggcagc gccagcgaca
    22501 atttacccgc gactggcaat ctgcgctgga agcactggcg gcgcttttac ccgagcacgc
    22561 ctggctgaca acgataagct ggcagcaggg aacgctggag atcaaggggc tgacaacaag
    22621 cattaccgcg ttaaacgcac tagaaacgtc actccgccag gatgcgtctt ttcatctcaa
    22681 tcagcgggga gccacgcagc aggatgcgca gggacgctgg caatttgagt atcagttaac
    22741 aaggaaggtt agcgatgaac atgttctttg actggtggtt cgccacatca ccccgcctcc
    22801 gccagttttg ctgggcattc tggttgctga tgttagttac gctcattttt ctatcatcga
    22861 cacaccatga agagcgcgac gcattaattc gactacgggc aagtcatcac cagcagtggg
    22921 ccgcactgta tcgcctggta gacaccactc ccttcagcga ggaaaaaacg ctgccctttt
    22981 cgccactgga ttttcagtta tccggcgcgc aactggtttc ctggcatcca tccgcgcagg
    23041 gaggtgagtt ggcgttgaaa acgctgtggg aagcagtgcc gtcggcattt acacggctgg
    23101 cagagcgcaa cgtcagcgtg agccgttttt cgttaagcgt ggaaggtgat gatcttttgt
    23161 tcacgctaca actggagatg ccgcatgagg gttaaacgct ggttgttggc aggtattgca
    23221 ttgtgccttt taaccggtat gcgtgaccct tttaaatcgc cggaagatct atatgccgga
    23281 ttagcgaact tagccagtgg cgctatcagg ggatggtagg gcgaggcgag cgcatcatcg
    23341 gtgtaataaa agacgggcaa aagaaatggc gacgggtgca gcaaaacgat gtgctggaaa
    23401 acggctggac aattttacag ctgacgccag acgtactaac gctgggtacc gggacaaact
    23461 gcgaaccgcc acaatggttg tggcaacggc aaggagatac aaatgaagca atggatagcc
    23521 gcactactgt tgatgctgat acccggcgta caggcggcaa agccgcaaaa agtgacgctg
    23581 atggtggatg acgttccggt agctcaggtg ttgcaggcgc tggctgaaca ggagaagttg
    23641 aacctggtcg tgtcgccaga cgtcagcggt acggtgtcgt tacatctaac agatgttccc
    23701 tggaagcagg cactacaaac tgtagtgaaa agcgccggac tgataacgcg gcaggaaggc
    23761 aacattctct cagtgcattc cattgcctgg cagaatgaca atatcgcccg ccaggaggcg
    23821 gagcaggcgc gggcgcaggc aaatctgccg ctggaaaatc gcagtataac cctgcaatac
    23881 gccgacgcgg gagaactggc gaaagcgggg gagaagctac tgagtgccaa agggagtatg
    23941 accgtcgata aacgcaccaa tcgccttttg ctacgagata acaaaacggc gttaagcgcg
    24001 cttgaacagt gggtagcgca aatggatctg ccggtcgggc aggttgagct gtcggcgcat
    24061 attgtcacca ttaatgaaaa aagtttgcgt gagttaggcg tgaaatggac gctggccgat
    24121 gcgcaacacg ctggtggcgt tgggcaagtc accacgcttg gtagcgacct ctccgtagcg
    24181 acggcgacaa cgcatgtcgg ttttaacatt gggcgcatca acggacgctt gctggatctt
    24241 gagctttccg cgctcgaaca aaaacagcag ctggatatta tcgccagtcc gcgtctgctg
    24301 gcctcacatc ttcagcctgc cagcattaaa caggagagcg aaattccata tcaggtttcc
    24361 agcggggaaa gtggcgcgac gtcggtggaa tttaaagagg ccgtcctggg gatggaggtc
    24421 acgcccacgg tgttacaaaa aggtcgcatc cggctgaaat tacacatcag ccagaacgtt
    24481 ccggggcagg tgctacagca ggccgatggc gaagtgctgg cgattgataa gcaggagatc
    24541 gaaacgcagg tcgaggtaaa aagcggagaa acgttggcgc tgggcggcat ttttacccgt
    24601 aaaaataaat cgggtcagga tagcgtaccg ttgcttggcg acattccctg gttcgggcaa
    24661 ttatttcgtc atgacggaaa agaagatgaa cgacgcgagt tagtggtgtt tatcacgcca
    24721 cgactggttt ccagtgagta aacagccgta aaagcggtaa tgtttttatg ctgaacgtgt
    24781 ttcatctatt tgacgcgcgc aggtatttag catacaagga gtaccgattt gagagttggt
    24841 gctcttcgct gcctgcgttc catgatgatg atttatcatt caggcggcat tttgctgtct
    24901 tttttacgct aatcttaccc ggtgatttat cgccagagcg gtggtagcaa ggcagcgcgc
    24961 ttgcagcgac cagatatgca gagggatggg tgatttattc agttgccaaa cccgctggag
    25021 tattgagata attttcagtc tgactctcgc aatatcttat gaggtttcag ttcatgtcct
    25081 gcggcgctct ctgagcgaag cgggtttatc attaacgaat agtcttagta gtaccgaaaa
    25141 aatggcagag aaacgcaata tctttctggt tgggcctatg ggtgccggaa aaagcactat
    25201 tgggcgccag ttagctcaac aactcaatat ggaattttac gattccgatc aagagattga
    25261 gaaacgaacc ggagctgatg tgggctgggt tttcgattta gaaggcgaag aaggcttccg
    25321 cgatcgcgaa gaaaaggtca tcaatgagtt gaccgagaaa cagggtattg tgctggctac
    25381 tggcggcggc tctgtgaaat cccgtgaaac gcgtaaccgt ctttccgctc gtggcgttgt
    25441 cgtttatctt gaaacgacca tcgaaaagca acttgcacgc acgcagcgtg ataaaaaacg
    25501 cccgttgctg cacgttgaaa caccgccgcg tgaagttctg gaagcgttgg ccaatgaacg
    25561 caatccgctg tatgaagaga ttgccgacgt gaccattcgt actgatgatc aaagcgctaa
    25621 agtggttgca aaccagatta ttcacatgct ggaaagcaac taattctggc tttatataca
    25681 ctcgtctgcg ggtacagtaa ttaaggtgga tgtcgcgtta tggagaggat tgtcgttact
    25741 ctcggggaac gtagttaccc aattaccatc gcatctggtt tgtttaatga accagcttca
    25801 ttcttaccgc tgaaatcggg cgagcaggtc atgttggtca ccagcgaaac cctggctcct
    25861 ctgtatctcg ataaggtccg cggcgtactt gaacaggcgg gtgttaacgt cgatagcgtt
    25921 atcctccctg acggcgagca gtataaaagc ctagctgtac tcgataccgt ctttacggcg
    25981 ttgttacaaa agccgcatgg tcgcgatact acgctggtgg cgcttggcgg cggcgtagtg
    26041 ggcgatctga ccggcttcgc ggcggcgagt tatcagcgcg gtgttcgttt cattcaagtc
    26101 ccgacgacgt tactgtcgca ggtcgattcc tccgttggcg gcaaaactgc ggtcaaccat
    26161 cccctcggta aaaacatgat tggcgcgttc taccagcctg cttcagtggt ggtggatctc
    26221 gactgtctga aaacgcttcc cccgcgtgag ttagcgtcgg ggctggcaga agtcatcaaa
    26281 tacggcatta ttcttgacgg tgcgtttttt aactggctgg aagagaatct ggatgcgttg
    26341 ttgcgtctgg acggtccggc aatggcgtac tgtattcgcc gttgttgtga actgaaggca
    26401 gaagttgtcg ccgccgacga gcgcgaaacc gggttacgtg ctttactgaa tctgggacac
    26461 acctttggtc atgccattga agctgaaatg gggtatggca attggttaca tggtgaagcg
    26521 gtcgctgcgg gtatggtgat ggcggcgcgg acgtcggaac gtctcgggca gtttagttct
    26581 gccgaaacgc agcgtattat aaccctgctc acgcgggctg ggttaccggt caatgggccg
    26641 cgcgaaatgt ccgcgcaggc gtatttaccg catatgctgc gtgacaagaa agtccttgcg
    26701 ggagagatgc gcttaattct tccgttggca attggtaaga gtgaagttcg cagcggcgtt
    26761 tcgcacgagc ttgttcttaa cgccattgcc gattgtcaat cagcgtaaca acaagaaagg
    26821 tcaggccgct tatcaagcgg tctattagct tcaggttaat tgcaacgtgg taagcattaa
    26881 ccttttagtg gggtgttaaa tggatgaatt caaaccagaa gacgagctga aacccgatcc
    26941 cagcgatcgt cgtactggtc gttctcgtca atcttctgaa cgttctgagc gtactgaacg
    27001 tggcgaaccg cagatcaatt ttgatgatat tgaacttgat gacactgacg atcgccgtcc
    27061 gactcgtgcg caaaaagagc gcaatgagga accggaaatc gaagaagaaa ttgacgaatc
    27121 cgaagatgaa accgtggatg aagagcgcgt agagcgtcgt ccgcgtaagc gcaaaaaagc
    27181 agccagtaaa cccgcttctc gtcagtatat gatgatgggc gtcggcattc tggttctact
    27241 gctgttgatc atcggtatcg gttctgcgct aaaagccccc tcgaccactt ccagcgatca
    27301 aaccgcgtct ggcgagaaga gtattgatct tgctggcaat gcgaccgatc aggcgaatgg
    27361 cgtgcagcca gcgccgggaa ccacgtctgc ggaaaatact cagcaggatg tttctctgcc
    27421 accgatctct tctacgccga ctcaagggca aaccccggtg gcaacggatg gtcaacaacg
    27481 tgttgaagtg cagggtgacc tgaacaatgc gctgacccag ccacaaaatc agcaacagct
    27541 gaacaatgtg gcggtcaatt ccacgttgcc gactgaaccc gcaacggttg cgcctgttcg
    27601 caatggcaat gcatcgcgtg acacggcgaa aacgcaaacc gctgaacgtc cgtccactac
    27661 gcgcccagtt cgtcagcagg cggtgattga accgaaaaaa ccgcaagcaa ccgtgaaaac
    27721 ggagccgaag ccggtagcac agacgccgaa gcgtactgaa cctgaaccag ctgcccctgt
    27781 ggcgagcacg aaggcaccgg ctgcgacttc tacgccagca ccaaaagaga cggcgactac
    27841 ggctccagta cagacggcat ccccggcgca aaccacggca acaccagccg ctggagggaa
    27901 gaccgcaggt aatgttggtt cgttgaaatc ggcaccgtcc agccattaca ctctgcagct
    27961 gagcagttcc tctaactacg acaacctgaa cggttgggcg aagaaagaga atctgaaaaa
    28021 ctacgttgtc tatgaaacga cgcgtaatgg tcagccgtgg tatgtcctgg tttctggcgt
    28081 gtacgcttcg aaagaagagg cgaaaaaagc ggtatctaca ttgccagcag atgttcaggc
    28141 caaaaacccg tgggcgaaac cgctgcgtca ggtacaggcc gatctgaagt aatcaaggtt
    28201 atctcccgca atggtttatc gttgcgggag ctgcctgaag cgctggatgc tgtcggagct
    28261 ttctccacag ccggagaagg tgtaattagt taatcggcat gaagaaaaat cgcgcttttt
    28321 tgaagtgggc agggggcaag tatcccctgc ttgatgatat taaacggcat ttgcccaagg
    28381 gcgaatgtct ggttgagcct tttgtaggtg ccgggtcggt gtttctcaac accgactttt
    28441 ctcgttatat ccttgccgat atcaatagcg acctgatcag tctctataac attgtgaaga
    28501 tgcgtactga tgagtacgta caggccgcac gcgagctgtt tgttcccgaa acaaattgcg
    28561 ccgaggttta ctatcagttc cgcgaagagt tcaacaaaag ccaggatccg ttccgtcggg
    28621 cggtactgtt tttatatttg aaccgctacg gttacaacgg cctgtgtcgt tacaatctgc
    28681 gcggtgagtt taacgtgccg tttggccgct acaaaaaacc ctatttcccg gaagcagagt
    28741 tgtatcactt cgctgaaaaa gcgcagaatg cctttttcta ttgtgagtct tacgccgata
    28801 gcatggagcg cgcagatgat gcatccgtcg tctattgcga tccgccttat gcaccgctgt
    28861 ctgcgaccgc caactttacg gcgtatcaca caaacagttt tacgcttgaa caacaagcgc
    28921 atctggcgga gatcgccgaa ggtctggttg agcgccatat tccagtgctg atctccaatc
    28981 acgatacgat gttaacgcgt gagtggtatc agcgcgcaaa attgcatgtc gtcaaagttc
    29041 gacgcagtat aagcagcaac ggcggcacac gtaaaaaggt ggacgaactg ctggctttgt
    29101 acaaaccagg agtcgtttca cccgcgaaaa aataattctc aaggagaagc ggatgaaaca
    29161 gtatttgatt gccccctcaa ttctgtcggc tgattttgcc cgcctgggtg aagataccgc
    29221 aaaagccctg gcagctggcg ctgatgtcgt gcattttgac gtcatggata accactatgt
    29281 tcccaatctg acgattgggc caatggtgct gaaatccttg cgtaactacg gcattaccgc
    29341 ccctatcgac gtacatctga tggtgaaacc cgtcgatcgc attgtgcctg atttcgctgc
    29401 cgctggtgcc agcatcatta cctttcatcc agaagcctcc gagcatgttg accgcacgct
    29461 gcaactgatt aaagaaaatg gctgtaaagc gggtctggta tttaacccgg cgacacctct
    29521 gagctatctg gattacgtga tggataagct ggatgtgatc ctgctgatgt ccgtcaaccc
    29581 tggtttcggc ggtcagtctt tcattcctca aacactggat aaactgcgcg aagtacgtcg
    29641 ccgtatcgac gagtctggct ttgacattcg tctggaagtg gacggtggcg tgaaggtgaa
    29701 caacattggc gaaatcgctg cggcgggcgc ggatatgttc gtcgccggtt cggcaatctt
    29761 cgaccagcca gactataaaa aagtcattga tgaaatgcgc agtgaactgg caaaggtaag
    29821 tcatgaataa gtttgaagat attcgcggcg tcgcttttga tcttgatggt acgctggtcg
    29881 acagtgctcc tggtcttgct gctgcggtag atatggcgct gtatgcgctg gagttgcccg
    29941 tcgcaggtga agaacgcgtt attacctgga ttggtaacgg cgcagatgtt ctgatggagc
    30001 gcgcattgac ctgggcgcgt caggaacgtg cgactctgcg taaaacaatg gggaaaccgc
    30061 ccgttgatga cgacattccg gcagaagaac aggtacgtat tctgcgtaaa ctgttcgatc
    30121 gctactatgg cgaggttgcc gaagagggga cgtttttgtt cccgcacgtt gccgatacgt
    30181 tgggcgcgtt gcaggctaaa ggcctgccgc taggcttggt caccaacaaa ccgacgccgt
    30241 tcgtcgcgcc gctgctcgaa gccttagata ttgccaaata ctttagcgtg gttatcggcg
    30301 gcgatgatgt gcaaaacaaa aaaccgcatc cggacccgct gttactggtg gctgagcgga
    30361 tgggaattgc cccacaacag atgctgtttg tcggcgactc acggaatgat attcaggcgg
    30421 caaaagcggc aggttgccca tcagttggct taacctacgg atacaactac ggcgaggcta
    30481 tcgatctcag ccagcctgat gtaatttatc agtctataaa tgaccttctg cccgcattag
    30541 ggcttccgca tagcgaaaat caggaatcga aaaatgacta agcccatcgt ttttagtggc
    30601 gcacagccct caggtgaatt gaccattggt aactacatgg gtgcgctgcg tcagtgggta
    30661 aacatgcagg atgactacca ttgcatttac tgtatcgttg accaacacgc gatcaccgtg
    30721 cgccaggatg cacagaagct gcgtaaagcg acgttggata cgctggcctt gtatctggct
    30781 tgtggtatcg atcctgagaa aagcaccatt tttgttcagt cccacgtacc agaacatgcg
    30841 cagttaggct gggcactgaa ctgctatacc tacttcggcg aactgagtcg catgacgcag
    30901 tttaaagata aatctgcgcg ttatgccgag aacatcaacg ctggtctgtt tggctatccg
    30961 gtgctgatgg ctgcggacat cctgctgtat caaactaatc tggtaccggt gggtgaagac
    31021 cagaaacagc acctggaact gagtcgcgat atcgcccagc gtttcaacgc gctgtatggc
    31081 gagatcttta aggtgccgga gccgtttatt ccgaaatctg gcgcgcgcgt aatgtcgctg
    31141 ctggagccga ccaagaagat gtccaagtct gacgataacc gcaataacgt tatcggcctg
    31201 ctggaagatc cgaaatcggt agtgaagaaa atcaaacgcg cggtcactga ctccgacgag
    31261 ccgccggtag ttcgctacga tgtgcagaac aaagcgggcg tttccaacct gctggatatc
    31321 ctttctgcgg taacgggcca gagcatccca gaactggaaa aacagttcga aggcaagatg
    31381 tatggtcatc tgaaaggtga agtggctgat gccgtttccg gtatgctgac tgaattgcag
    31441 gaacgctatc accgtttccg caacgatgaa gccttcctgc aacaggtgat gaaagacggc
    31501 gcagaaaaag ccagtgtaca cgcttcccgt acgctgaaag cggtgtacga agcgattggt
    31561 tttgtggcga agccgtaata tcaaaacgct cggacggtcc cctcgcccct ttggggagag
    31621 ggttagggtg aggggagcag gcaagcgcca gtgggaatta ccctttgctc gcattgtcga
    31681 ataaatcact tcgtcatcac tctgaaaccg gggaaacccg gtttttttat cctcttttcg
    31741 caacaacccg aaaaaatgtg aagcgcctcg ccgtttccac atctcgtcgt cgccacttat
    31801 cttcatttta atgacgatat aaatattcat tttattgaaa atatattatg cgtcgaacgt
    31861 ttatcaaaaa agaaggcgtc gtcatcacga cactggcccg ttatttgttg ggtgaaaaat
    31921 gcggtaatcg actgaaaacc atagatgagc tggcaactga atgccgttca tcggttggcc
    31981 tgacgcaggc cgcgttgaaa acgctggaat caagcggagc gatacggatt gaacgccgtg
    32041 ggcgcaatgg cagttatctt gtcgagatgg ataacaaagc attgctgagt catgtggata
    32101 tcaacaacgt ggtatgtgcg atgcctttgc cctatacccg cttgtacgaa ggtttagcga
    32161 gcggattgaa agcccagttt gatggcattc ctttttacta tgcgcatatg cgcggcgcgg
    32221 atattcgcgt ggagtgttta cttaacggtg tctatgacat ggcagtagtt tcccgcctgg
    32281 cggcggaaag ttatctcagc caaaacaatt tgtgcatcgc cctcgagctt ggcccgcata
    32341 cgtatgttgg tgaacatcaa ctgatatgcc gcaaaggtga gtctggaaat gttaagcgag
    32401 taggtctcga tagtcgttcg gcggatcaga aaatcatgac cgatgtcttt tttggcgata
    32461 gtgatgtgga acgagtcgat ctctcttatc acgagagttt acaacgcatt gttaaaggcg
    32521 atgtcgatgc ggtgatctgg aacgtggtgg cggaaaacga actgaccatg ctgggattag
    32581 aggcgacgcc gctaacagac gatccgcgat ttttacaggc caccgaagcc gtggtcctga
    32641 cgcgagtcga tgattaccca atgcaacaat tgctgcgtgc tgttgtagat aaacacgccc
    32701 tgcttgccca tcaacaacgg gtggtgagtg gggaacagga acccagttat taaacgaaag
    32761 gcatctgata tggaaaccag actcaacctg ctttgcgagg caggcgttat tgataaggac
    32821 gtctgcaaag gcatgatgca ggtcgtcaac gtactggaaa aagagtgcca tctgccggtg
    32881 cgcagtgagc aaggaacgat ggcgatgaca catatggcga gtgcactgat gcgcagtcgc
    32941 cgtggtgaag aaatagagcc gctggataac gagttgctgg cggaactggc gcaatccagc
    33001 cactggcaag ccgttgtgca attgcatcag gagttgttga aggaattcgc tctggaagtt
    33061 aacccgtgtg aagaaggcta ttttctggcc aatctttatg gattatggat ggctgctaac
    33121 gaagaggttt gaagctcccg acaaatccac gtcgcacaac ctaaatattc aaaaaaatga
    33181 atatttaatt caaggaacag aaacgaggca acaagatgtt tgtagaagca ttgaaacgcc
    33241 agaacccggc gctgatttcc gccgcactaa gtttgtggca gcagggcaaa atcgccccgg
    33301 acagctgggt gatcgacgtg gatcagatac tggaaaacgg taagcggctc attgagacgg
    33361 cgcggcttta cggcattgaa ctgtatctga tgaccaagca atttggtcgt aatccgtggc
    33421 tggcggaaaa attgctggca ttaggctaca gcggcattgt ggcggtggat tacaaagagg
    33481 cgcgagtcat gcgccgcgct ggtttgccag tggcgcatca ggggcatctg gtacaaatcc
    33541 cttgtcatca ggttgctgac gccgttgaac agggcaccga catcatcacc gtgtttactc
    33601 tcgacaaagc gcgggaagtt tctgcggcag cggttaaggc cggacgggtc cagcctgtgc
    33661 tgctcaaagt ttatagcgac gatgattttc tttatccggg gcaggagagc ggttttgtac
    33721 agcattcgct gcacgaggtg attgctgaaa ttaagaaact gccggggctg catttagccg
    33781 gactaaccca tttcccttgc ctgctttggg atgaggctgc tgggaaagtt ttgccaacac
    33841 cgaatcttca cacgctggta caagcacggg atcaactggc gaaatctggt attgcgattg
    33901 agcaactgaa cgtaccttca gcgaccagct gcacttcgct gccattgctg gcggaatacg
    33961 gtgcgactca tgccgaaccc ggtcatgcgc tgaccggtac tattccggca aaccagcagg
    34021 gcgatcagcc tgaacgtatc gcgatgctct ggttaagtga aatctcccat catttccgtg
    34081 gcgacagcta ctgctacggc ggcggttact accgtcgtgg tcatgcgcaa catgcgctgg
    34141 tgtttacgcc agaaaatcaa aagattactg aaaccaatct caaaactgtg gatgacagca
    34201 gtatcgacta caccctgccg ctggcaggcg agtatccggt gagcagtgca gtggtgctct
    34261 gttttcgcac gcagattttt gtcacccgta gcgatgtggt gctggtgtcc ggtattcagc
    34321 gcggcgaacc agaaatagtt ggtcgttatg acagcctcgg caaccctctg gaggcataaa
    34381 tggcgcgatt tgtggtgtta gtgattgata gctttggcgt aggggcaatg aaagatgtca
    34441 cgctggtgcg cccgcaagat gccggggcga atacctgtgg tcacatcctg agccagttgc
    34501 cgcatttgca gctaccaacg ctggagaagc tggggctaat caacacattg ggttatgcgc
    34561 caggcgatat gcagccgtca gattccgcaa cctggggcgt ggcagagctg caacatgaag
    34621 gtggcgatac ctttatgggg catcaggaaa ttttaggcac gcgcccgtta ccgccgctgc
    34681 ggatgccttt tcgcgatgtg attgaccgtg ttgagcaggc attagtttcc gctggctggc
    34741 aggtggagcg ccgtggcgat gatctgcaat ttctgtcggt caatcaggcg gttgcgattg
    34801 gcgataatct cgaggcggat ttaggccaag tctataacat taccgccaat ctctctgtga
    34861 tctcttttga cgacgcaatc aaaattggtc gtatcgtgcg tgaccaggta caggtcggtc
    34921 gggtcattac atttggcggc ctgttaacca acagtcagcg tattctcgat gccgcagaaa
    34981 gcaaagaagg gcgctttatt ggtattaatg cgccgcgttc tggcgcttat gacaacggct
    35041 tccaggtcgt gcatatgggc tacggtgttg atgaaaaagt gcaggtgccg caaaaactgt
    35101 atgaagcggg cgtgccaacc gtgctggtgg gtaaggtggc agatatcgtc agcaatcctt
    35161 atggtgtgag ctggcaaaac ctggtggata gccagcggat tatggatatc accctcgacg
    35221 aatttaacac ccatccgacg gcgtttattt gcatcaatat ccaggaaacc gacctcgctg
    35281 gtcatgcaga agacgtcgca cgttatgccg aacgtttgca ggtcgttgac cgtaaccttg
    35341 cccggcttgt tgaggcgatg cagccagatg attgcctggt cgtgatggcg gatcacggta
    35401 acgatccgac cattggtcac agccaccata cccgtgaagt ggtgccagtg ctggtttatc
    35461 agcaagggct ggtcgccacg cagctcggtg tgcgcaccac gctttctgat gtgggagcta
    35521 ccgtgtgtga atttttccgt gcgccaccgc cacaaaatgg tcgctctttt ctttcctccc
    35581 tccggtttgc aggagacacc ctatgagttt tgatccgacg ggttacaccc tggcccatga
    35641 gcatctgcat attgatctct ccggctttaa aaacaacgtg gactgccgcc ttgatcagta
    35701 tgcgttcatt tgccaggaga tgaacgacct gatgacccgg ggtgtacgta atgtgattga
    35761 gatgaccaac cgttacatgg ggcgcaatgc gcaatttatg ctcgatgtaa tgcgcgaaac
    35821 cgggatcaac gtggtggcct gtactggtta ttaccaggat gcgtttttcc cggaacatgt
    35881 ggtgacccgc agcgtgcagg aactggcgca ggagatggtc gatgaaattg aacagggtat
    35941 cgatggcacg gagctgaaag ccgggatcat cgcggagatc ggctccagcg aaggaaagat
    36001 tacgccgctg gaagagaagg tatttattgc cgctgcgctg gcgcataacc agaccggacg
    36061 cccgatctcc acgcatacgt cgttcagcac catggggctg gaacaactgg ctctgttgca
    36121 agcccacgga gttgatcttt cgcgcgtcac cgttggtcac tgcgatctga aagacaacct
    36181 cgacaacatt ttgaagatga tcgatctcgg cgcgtacgtg cagttcgaca ccattggtaa
    36241 gaacagttac tacccggacg aaaagcgcat tgcgatgctc cacgcgctac gtgaccgtgg
    36301 gttgctgaac cgcgtcatgc tgtcgatgga tattacgcgc cgctcccatt taaaagccaa
    36361 tggtggttat ggctatgact atttattgac cacctttatt ccgcaattgc gccagtcagg
    36421 attcagtcag gccgatgtgg atgtgatgtt acgtgaaaat ccctctcaat ttttccaata
    36481 aggacagact catgaaaaag attggcgttg caggcttaca gcgtgagcag attaaaaaaa
    36541 ccattgaagc gacggctcct ggctgttttg aagttttcat tcacaacgac atggaagcgg
    36601 caatgaaggt gaaatccggg caactagatt attacattgg cgcgtgtaat accggtgcgg
    36661 gcgcggcatt gtcgattgcc atcgcggtga ttggctataa caaaagttgc accattgcca
    36721 aaccaggcat taaagcgaaa gacgagcata tcgccaaaat gatcgctgaa gggaaagtgg
    36781 cgtttggcct ttccgttgag cacgtcgaac acgcgattcc gatgttgatt aaccatctga
    36841 aataaaaggc acgactatgg atctgtatat tcagattatc gtggtggcgt gcctgacggg
    36901 tatgacatcg cttctggagc atcgctcggc ggctgttttt catgacggca tccacccgat
    36961 cctgccgcaa ctgattgaag gctatatgaa ccgtcgcgag gcggggagta tcgcttttgg
    37021 tctgagcatt ggttttgtgg cctcggtggg gatctctttt accctgaaaa ccgggctgct
    37081 caacgcatgg ttactctttc ttcctaccga tatcctcggc gtactggcga taaacagcct
    37141 gatggcgttt ggtcttggcg ctatctgggg cgtgttgatc cttacttgcc tgttgccagt
    37201 taaccagctg ctgaccgcgc tgccggtgga tgtattaggt agcctcgggg aattaagctc
    37261 gccggtggtt tctgcttttg cactgttccc gctggtggcg attttctacc agtttggctg
    37321 gaagcaaagt ctggtcgccg ccgtggttgt tctgatgacc cgtgtggtag tcgtgcgcta
    37381 tttcccacat cttaaccctg aatccatcga aatctttatt ggcatggtga tgctgctggg
    37441 gatcgcgata actcacgacc tgcgtcatcg tgatgaaaat gacatcgatg ccagcgggct
    37501 ttcggtgttt gaagagcgca cgtcgcggat tatcaaaaac ttaccttata tcgccatcgt
    37561 gggagcattg attgccgccg ttgccagtat gaagattttt gctggtagcg aagtttcgat
    37621 cttcacactg gagaaagcgt attccgcagg cgtgacgccg gaacaatcgc aaacgctgat
    37681 taatcaggcg gctctggcag aatttatgcg cggactgggg tttgtgccgt tgattgccac
    37741 caccgcctta gcaacgggtg tgtatgcagt tgcgggcttt acctttgttt atgcggtggg
    37801 ctatctctcg ccgaatccga tggttgcagc ggtattaggc gcagtggtta tttcggcgga
    37861 agtcttgctg cttcgttcga tcggcaaatg gctgggacgc tacccgtcgg tgcgtaatgc
    37921 gtcggataac atccgtaacg ccatgaatat gctgatggaa gtggcgctgc tggtcggttc
    37981 gattttcgca gcaattaaga tggcgggtta taccggattc tctatcgcgg ttgccattta
    38041 cttcctcaac gaatccctgg gccgtccggt acagaaaatg gcggcaccgg tcgtggcagt
    38101 aatgatcacc ggtattctgc tgaatgttct ttactggctt ggcctgttcg ttccggctta
    38161 aggaaggcac ctatgaagac gtttcctttg caaagcctga ctcttgctga agcgcagcaa
    38221 aagcagtttg cgctggtgga cactatttgc cgccattttc caggcagcga gtttcttgct
    38281 ggcggtgatt tagggttaac tccaagtctg aatcaaccgc gtattaccca acgggtggag
    38341 caggtgctgg ctgatgcatt tcacgcacag gccgcggcgc tggtgcaggg cgcggggact
    38401 ggcgcgattc gcgccgggct ggcggctttg ctcaaaccgg ggcagcgtct tctggtgcat
    38461 gacgcgcctg tttacccgac gacacgggtt attattgagc agatggggct gacgcttatt
    38521 actgctgatt tcaatgacct gtcggcactg aagcaggtcg tcgacgagca acaacctgat
    38581 gccgcgctgg tgcagcatac gcgccagcag ccgcaggaca gctatgtgct ggcagatgtg
    38641 ctggcaacgt tgcgcgcggc aggtgttcca gcgttaaccg atgataacta tgcggtgatg
    38701 aaggtagcgc gaatcggctg tgaatgcggc gcgaatgtct cgacattctc ctgcttcaag
    38761 ctgtttgggc cggagggcgt tggtgcagtg gttggcgatg ccgatgttat tagtcggatt
    38821 cgcgccacgc tttactccgg cggtagccag atccagggct cacaggcgct ggaagtcttg
    38881 cgtggtctgg tgttcgcgcc agtgatgcat gcggtgcagg cgggagtatc cgaacggttg
    38941 ctggctttgc ttaacggcgg cgcggtggcg gaagtgaaaa gcgccgtcat tgccaatgcg
    39001 cagtcgaagg tgttgattgt cgagtttcat cagctgattg ccgccagggt gctggaagaa
    39061 gcgcaaaagc gtggcgcgtt gccttacccg gtcggtgcag agtcgaaata tgaaatcccg
    39121 ccgctcttct atcgactttc aggaacgttt cgccaggcga atccacaact cgaacattgt
    39181 gcgattcgca ttaacccgaa tcgcagcggt gaagagacgg tgctgcggat tttgcgtgag
    39241 agtattgcga gtatttaatg ctggatagca ccgttcggca atcaagctaa attccgcact
    39301 gaacggtccc ctcgcccctt tggggagagg gttagggtga ggggaacgtg cgggatgtaa
    39361 aattcagtgc gccagcatcg ccggatgcga tgctggcgca tcttatccgg cctacggtta
    39421 atgtctttta ttatcaatcg ttaaggtgat gcgattggcg cggcagaaga gttcggagac
    39481 gtgcaccggt ttgtcattct gatcaaaggc gattttgctg atgcgaaaca acggttcgcc
    39541 cagttcgcat ttcagccatt ttgcctgcgg gcgggtggcg gcaaagatat cgatggtctt
    39601 tttgtcgctg accactcgcg tgtcaaaacg ctcctgaaat aactgatagg tggagcttcc
    39661 ttcgacgtaa atctcatcaa agtcaggata acgcgacagc gggatccatg aactatcaat
    39721 aaacagcggc tctttatcga gatacatcac ccggcagaga tggaacactt cgctgtttcc
    39781 ggggatgttc agcttttcgc aaaacggcgc ggcgctgacc cgttcctgtt cgatcacttt
    39841 ctctttcgtc gacttgcctt gtgagacgcc aaaatcggta aaaccactga cagtaagcag
    39901 ggcgttttca actttctggc tttgtacaaa ggtgcctttt ccctgccagc ggatcagtac
    39961 gccgtctgcc actaagtcgc tgatggcttt gcgaatggta atgcggctga cgttatattg
    40021 tgtacaaagc tcgttttcgg tagggatctg ttgcccggcc tggtaaaccc cctgcgcgat
    40081 atcatccagc agccgctggc ggacggtagc gtagaggagt tgatgagagt agcggtccgt
    40141 agctgacata atcgaccctg aaataaagaa tgaattactc ggattatagg gtcgttttcc
    40201 ggcaagtaac agcacgttgg cgtgaagatg ctcacgccaa cgttatacct accaggcacc
    40261 gtggtactga atggttttcg ccgcgcacgc cgttccctgc gccatcgcct gcggtaatgt
    40321 catccccgca gaccagccgc aaaggaatcc ggcaatgaac gaatctccgg cacccatggt
    40381 gtcgataacc gtcaccggtt caggagcctg acgccagaac tgcgcgccat cccaggcaat
    40441 gctgccgttt tcacccagcg tgacaatcac tgttcctgcg ccacgggcaa caatcgcttt
    40501 catcttcaga cgcagcgctt cgtcttcttg cggtgcggag gcaaaggcaa aatcgagatg
    40561 cggcaccagt gtctgccaga gcgggctgtc ccacttgtcg gagaagtcga aagcggtaag
    40621 tttgcccgca gcgtgcagct gtgggaatgc gtcttccgca tgtccccaga ttgccgcgtg
    40681 cacaatgtca tactgcgcca gccaggcgta atcctcttca ctcagggcaa agtcggccat
    40741 cacgccttcg gtgtagtcgc caaaaacgcg atcattgtcg tgcagttcca cctgagtttg
    40801 tgcggtaacg ccgtgtttcg tatggacatg gctgatatcg acgcccatgc gggcgagatc
    40861 ctgcttcagc tttgtgccgt agtcatcgtc acccacccag gtaatgcatc ccggctgtat
    40921 gccgtagcga gtgcagtaca ccgccacatt gaccgcatta ccgccagaaa acgctttatt
    40981 cagttgcggg tagatatcga cgcagttatc gccgattgtc gccagggttt tcatctctca
    41041 tcctccggca gcagcgcgcg aaagcgttcc agcgcctggc gggcatagag tctgggctcg
    41101 ttcatataca tcgtcaccag ctccaccgta gagtaaccct catagccccg atcaataata
    41161 tcgcgcatca gttcccgcag cggcattttg ccttctccag gaatgtaatg cgtgtcgctg
    41221 gccccgtcgc tgtcgacaat atgcagatga cgtaatttat cgcccagttt gtcgaaataa
    41281 ctcatcaccg gttccgcctg gacatacggc gcgcaaatgt cgaccatgct gaacaggcgc
    41341 ggcgaaggca ccagcgccag cgcatgaagc acatcattag cattacacac gacgttcgat
    41401 tcatacggcg ttaacggttc gaggatcaga tccatgccga tgttttccgc gtactcacac
    41461 agttcgctta agttctctgc cagccgcccc cagataacgt taggcggcgt gagatagccc
    41521 gcgtgggccg cagaaatcag cgtataaccc gcgttcatct cttttgccat atctatcgcc
    41581 agcttgatca tgtccaggct ttcacgacgc atatgttcat cgcccagcat catgttatac
    41641 ggatagccgt tggtttctgg cgtgtaaccg ataatcggca tctgatacgt ctgcgccagc
    41701 gccttgattt gtttgatgcc gcccgctttt aagtccggcg cgaacgcgtg cgggcgaccg
    41761 ccccaaattt cgatgccgtc gtaacccagc tcgcttgcat cacgaaatgc gtgttcaata
    41821 ggcagccgct ggtggccgca ggtaaacata cctgttttca tcgggtaact ccttgcgtaa
    41881 atccggcccg ttgccgggcc gggagaggga ttaatattcc accagaccac cgtaatagcg
    41941 gcgttcatcc gggttgtgat ctttgtaaat agacaggtag tagcagagcc actccattgg
    42001 cacgaacatc aggaacggtg ccagccacgg gtgcagccct tgcgaaattt cggcgtaatc
    42061 gatgacgatc acgttgtcag tacgctgttt aacaaagtta atggcgcgtt cggtggtgtg
    42121 gcgactttca tcattgccga gcaggaacag gaacggaacg cccggttcga caatctccag
    42181 cgggccatgg cggaactctc cgctctcaat cacgcagccg tgcgtccagg taaattccat
    42241 cagcgttaca atgccttctt tgtaacccag cggacgcagc ggacccgcag caacggtata
    42301 aatcatcggc cactggctgg ccagttcatc aagctggcgg cctttttctt cccaggtgcg
    42361 taccagatga ccgagcgcat tcggcaactg cttgagatcg tttttgatct tgccgatttc
    42421 cgcgttcggc gcgaggcggg tgatcatctc cagcaccacg ctgtagcaga gcagcaggtg
    42481 aatttcccag atacagtcgg cctgataatc aatgctaaat tccgccgccg aggtaatcgg
    42541 gctgtccgcg cgtttggtga acgccgcagt gagtgctccg caggcccggc ccagctccag
    42601 cgctttgatt acctcttccg ttttaccgta gtcagaaacg ccaatcaccg cgcaacgatc
    42661 gtcgaggcga tacggggtgt tatcgcagaa ctcccagccg gaaatggcgt agacctgaag
    42721 atcggaaaag cgatccgcca gatgtttcgc cgtttgcgcc gcgttgagtg gcgagccgca
    42781 ggcaacgaaa taaatacgat caatgtcgcg cttcaccatc tcttccacga tggcgtgcac
    42841 cagcggaacg tcatggctga gaactttttc cacttcctga accatatttt cggtgaccag
    42901 aaagtccacg gtgcttttat caatatccaa cattttattt tcttcagata acattacgac
    42961 aaagcgttga gctggcggct acgcttcgcc cagaaggcgt aagcaggcag accagtagca
    43021 ataacgatgg cggcgcagat aaggccggga atcggtgccc agacaaaggt tgaggcgacc
    43081 agaatgaggc ttgacgcaat ggcgagggtg gtcatcagcc cgaaagccgg agtacgccac
    43141 agcggtttgt aatcgtcgcg tttacgacac cagatgatgg agccgaaggt gagggtattt
    43201 ttgaaacaca tcaccagggt gaaataaccc agcaggctgg tgagatcgga aacgaagatg
    43261 aagaagatcc ccagcgcccc ttgcaggatg atggagacat ccggcgtgtt gtatttcgga
    43321 tgcgcatggc cgaagcattt aaagaacagg ttgtctttcg ccatcgcgta ttccagccgc
    43381 ggctggtaca tcacgcagct ggaaagcgaa ccaagaatga cgatcatcgc cgtgatggca
    43441 acaaagagcc cagcggtgct gccgagtgcg gggatctagg tcagggcgtc ggaaatcggc
    43501 gtttcagaat tggcgagttt gtcgaagggc atcaggccgg aaatcaccag cgccagcagg
    43561 gtgtagagca ccagaaccag cagacaggaa ccaatcagcg ctcgtggcat ggtttttccg
    43621 gggtttttaa tttcgccggt catataacag atagaggcca tgccggtata cgaccaactg
    43681 gtggcagaga tccccgccag cagcgccata aagctgcccg ttgcgccaat cgcagtggtg
    43741 gtaagggcgg caaaattctc cgctttaaac cagaagatcc caaggccaat gacgatagtg
    43801 aacgggataa ttttggcgat ggtaattagc gtctgaaacg ctgcgccgcc ttcaacggag
    43861 cgcaggtgta gcaacataaa ggcgataatt aatccggcgg cgataaattt accgagcaac
    43921 ggatcgatag gcgttaaaaa gccaagattg ctgacaatcg ccagcgccat aatcgacaat
    43981 gacggcgcat cgttggccca gaagctggcc cagccggaga ggaaagccag cggtcggctt
    44041 ccggcatttt tcagataaac ataatctgcg ccattttccg gataagcggt ggatagttcc
    44101 gcatagacgc acatttgcgg gatcacaatt aacccgccaa tgacaaacgc gagcaccgta
    44161 agccacggcg tgcccgctgc ttttgccact tcacccacag atacaaaaat accggagccg
    44221 acggttgtcc cgacggcgat tgcaagaacg gcccaaaatc cgagcttgcg ttggagttcc
    44281 tggcttccca tagcgatacc tttttcttgt ccaatacgtt gtaggggaga gtttgcagcc
    44341 atttaaaagg taacatgaca atgcatgatg aatataacaa catattatgt tatgtgatgt
    44401 ggaggaagat cacaaaattt cgcacaggat cgcgctgtgg ctaatggatg tagttataaa
    44461 attgaattta aagtgaaaat atttttacgg gcgggggcaa gaaggacata gaaacaaata
    44521 cgccctcgga aaatccagag ggcgtcgggc aattaaaccg gtgttagccg atttctgtca
    44581 gagacttact gtgcagtagg accgcaaccg ccaatgattt tagaaataga aattgccagg
    44641 tgcagcaggt agtcgtaaga gcagttctta tcacggtttt caatatgacc agtacatgca
    44701 gttaacgtag ccagtactgc acctaccagt gcaactttaa taaatttgt
//
LOCUS       DAZXUL010000022        44359 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZXUL010000022 DAZXUL010000000
VERSION     DAZXUL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44359
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.022"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1739
                     /gene="adeD"
                     /locus_tag="ACH3L1_001123"
     CDS             <1..1739
                     /gene="adeD"
                     /locus_tag="ACH3L1_001123"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFT4075950.1"
                     /translation="HISRAEYQELLAVSRGDAVADYIIDNVSILDLINAGEISGPIVI
                     KGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGL
                     TTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQ
                     MLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGLGGKELNAYIA
                     AGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNSPQCMLCTDDR
                     NPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIV
                     LLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQPVSASDFALQF
                     TPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGF
                     GLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPI
                     AGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDG
                     EKFAFTTLEVTE"
     gene            complement(1785..3176)
                     /gene="uhpT"
                     /locus_tag="ACH3L1_001124"
     CDS             complement(1785..3176)
                     /gene="uhpT"
                     /locus_tag="ACH3L1_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFT4075951.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3314..4633)
                     /gene="uhpC"
                     /locus_tag="ACH3L1_001125"
     CDS             complement(3314..4633)
                     /gene="uhpC"
                     /locus_tag="ACH3L1_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFT4075952.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4643..6145)
                     /gene="uhpB"
                     /locus_tag="ACH3L1_001126"
     CDS             complement(4643..6145)
                     /gene="uhpB"
                     /locus_tag="ACH3L1_001126"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFT4075953.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6145..6735)
                     /gene="uhpA"
                     /locus_tag="ACH3L1_001127"
     CDS             complement(6145..6735)
                     /gene="uhpA"
                     /locus_tag="ACH3L1_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFT4075954.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6810..7100)
                     /gene="ilvN"
                     /locus_tag="ACH3L1_001128"
     CDS             complement(6810..7100)
                     /gene="ilvN"
                     /locus_tag="ACH3L1_001128"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFT4075955.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7104..8792)
                     /gene="ilvB"
                     /locus_tag="ACH3L1_001129"
     CDS             complement(7104..8792)
                     /gene="ilvB"
                     /locus_tag="ACH3L1_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFT4075956.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8898..8996)
                     /gene="ivbL"
                     /locus_tag="ACH3L1_001130"
     CDS             complement(8898..8996)
                     /gene="ivbL"
                     /locus_tag="ACH3L1_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFT4075957.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9561..9650
                     /gene="tisB"
                     /locus_tag="ACH3L1_001131"
     CDS             9561..9650
                     /gene="tisB"
                     /locus_tag="ACH3L1_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFT4075958.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9774..9848)
                     /gene="ysdE"
                     /locus_tag="ACH3L1_001132"
     CDS             complement(9774..9848)
                     /gene="ysdE"
                     /locus_tag="ACH3L1_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFT4075959.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9930..11114
                     /gene="emrD"
                     /locus_tag="ACH3L1_001133"
     CDS             9930..11114
                     /gene="emrD"
                     /locus_tag="ACH3L1_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFT4075960.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11122..11619)
                     /locus_tag="ACH3L1_001134"
     CDS             complement(11122..11619)
                     /locus_tag="ACH3L1_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFT4075961.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11616..11978)
                     /locus_tag="ACH3L1_001135"
     CDS             complement(11616..11978)
                     /locus_tag="ACH3L1_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFT4075962.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11968..12315)
                     /locus_tag="ACH3L1_001136"
     CDS             complement(11968..12315)
                     /locus_tag="ACH3L1_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFT4075963.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12424..12837
                     /gene="yidI"
                     /locus_tag="ACH3L1_001137"
     CDS             12424..12837
                     /gene="yidI"
                     /locus_tag="ACH3L1_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFT4075964.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12884..14326)
                     /locus_tag="ACH3L1_001138"
                     /pseudo
     CDS             complement(12884..14326)
                     /locus_tag="ACH3L1_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(14361..16076)
                     /locus_tag="ACH3L1_001139"
     CDS             complement(14361..16076)
                     /locus_tag="ACH3L1_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFT4075965.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            16243..17136
                     /locus_tag="ACH3L1_001140"
     CDS             16243..17136
                     /locus_tag="ACH3L1_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFT4075966.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17133..17947)
                     /locus_tag="ACH3L1_001141"
                     /pseudo
     CDS             complement(17133..17947)
                     /locus_tag="ACH3L1_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17947..19563)
                     /locus_tag="ACH3L1_001142"
     CDS             complement(17947..19563)
                     /locus_tag="ACH3L1_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFT4075967.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            19858..20532
                     /locus_tag="ACH3L1_001143"
     CDS             19858..20532
                     /locus_tag="ACH3L1_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFT4075968.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(20571..22232)
                     /locus_tag="ACH3L1_001144"
     CDS             complement(20571..22232)
                     /locus_tag="ACH3L1_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFT4075969.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22428..22856)
                     /gene="ibpB"
                     /locus_tag="ACH3L1_001145"
     CDS             complement(22428..22856)
                     /gene="ibpB"
                     /locus_tag="ACH3L1_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFT4075970.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(22968..23381)
                     /gene="ibpA"
                     /locus_tag="ACH3L1_001146"
     CDS             complement(22968..23381)
                     /gene="ibpA"
                     /locus_tag="ACH3L1_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFT4075971.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23375..23447)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23686..24018
                     /locus_tag="ACH3L1_001147"
     CDS             23686..24018
                     /locus_tag="ACH3L1_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFT4075972.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24020..25252)
                     /locus_tag="ACH3L1_001148"
     CDS             complement(24020..25252)
                     /locus_tag="ACH3L1_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFT4075973.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            25353..26453
                     /gene="cbrA"
                     /locus_tag="ACH3L1_001149"
     CDS             25353..26453
                     /gene="cbrA"
                     /locus_tag="ACH3L1_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFT4075974.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(26414..27751)
                     /locus_tag="ACH3L1_001150"
     CDS             complement(26414..27751)
                     /locus_tag="ACH3L1_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4075975.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(27826..28974)
                     /gene="dgoD"
                     /locus_tag="ACH3L1_001151"
                     /pseudo
     CDS             complement(27826..28974)
                     /gene="dgoD"
                     /locus_tag="ACH3L1_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(28971..29588)
                     /gene="dgoA"
                     /locus_tag="ACH3L1_001152"
     CDS             complement(28971..29588)
                     /gene="dgoA"
                     /locus_tag="ACH3L1_001152"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFT4075976.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29572..30450)
                     /gene="dgoK"
                     /locus_tag="ACH3L1_001153"
     CDS             complement(29572..30450)
                     /gene="dgoK"
                     /locus_tag="ACH3L1_001153"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFT4075977.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30447..31136)
                     /gene="dgoR"
                     /locus_tag="ACH3L1_001154"
     CDS             complement(30447..31136)
                     /gene="dgoR"
                     /locus_tag="ACH3L1_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFT4075978.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31414..32070
                     /gene="yidX"
                     /locus_tag="ACH3L1_001155"
     CDS             31414..32070
                     /gene="yidX"
                     /locus_tag="ACH3L1_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFT4075979.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32116..32928)
                     /gene="yidA"
                     /locus_tag="ACH3L1_001156"
     CDS             complement(32116..32928)
                     /gene="yidA"
                     /locus_tag="ACH3L1_001156"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFT4075980.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33042..33440)
                     /locus_tag="ACH3L1_001157"
     CDS             complement(33042..33440)
                     /locus_tag="ACH3L1_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFT4075981.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(33680..36094)
                     /gene="gyrB"
                     /locus_tag="ACH3L1_001158"
     CDS             complement(33680..36094)
                     /gene="gyrB"
                     /locus_tag="ACH3L1_001158"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFT4075982.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36123..37196)
                     /gene="recF"
                     /locus_tag="ACH3L1_001159"
     CDS             complement(36123..37196)
                     /gene="recF"
                     /locus_tag="ACH3L1_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFT4075983.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37196..38296)
                     /gene="dnaN"
                     /locus_tag="ACH3L1_001160"
     CDS             complement(37196..38296)
                     /gene="dnaN"
                     /locus_tag="ACH3L1_001160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFT4075984.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38301..39704)
                     /gene="dnaA"
                     /locus_tag="ACH3L1_001161"
     CDS             complement(38301..39704)
                     /gene="dnaA"
                     /locus_tag="ACH3L1_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFT4075985.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40001..40081
                     /locus_tag="ACH3L1_001162"
     CDS             40001..40081
                     /locus_tag="ACH3L1_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4075986.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40311..40451
                     /gene="rpmH"
                     /locus_tag="ACH3L1_001163"
     CDS             40311..40451
                     /gene="rpmH"
                     /locus_tag="ACH3L1_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFT4075987.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40468..40827
                     /gene="rnpA"
                     /locus_tag="ACH3L1_001164"
     CDS             40468..40827
                     /gene="rnpA"
                     /locus_tag="ACH3L1_001164"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFT4075988.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            40791..41048
                     /gene="yidD"
                     /locus_tag="ACH3L1_001165"
     CDS             40791..41048
                     /gene="yidD"
                     /locus_tag="ACH3L1_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFT4075989.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41051..42697
                     /gene="yidC"
                     /locus_tag="ACH3L1_001166"
     CDS             41051..42697
                     /gene="yidC"
                     /locus_tag="ACH3L1_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFT4075990.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            42803..44167
                     /gene="mnmE"
                     /locus_tag="ACH3L1_001167"
     CDS             42803..44167
                     /gene="mnmE"
                     /locus_tag="ACH3L1_001167"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFT4075991.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        10749 a        12030 c        11349 g        10231 t
ORIGIN      
        1 atcacattag ccgggctgaa taccaggaat tgttagccgt ttcccgtggc gacgctgttg
       61 ccgattatat tattgataat gtctctattc tcgacctgat caatgccgga gaaatttccg
      121 gcccaattgt gattaaagga cgttacattg ccggtgttgg cgcagaatac gctgatactc
      181 cggctttgca gcggattgat gctcgcggcg caacggcggt gccagggttt attgatgctc
      241 acctgcatat tgaatccagc atgatgacgc cggtcacttt tgaaaccgct accctgccgc
      301 gcggcctgac gaccgttatt tgcgaccctc atgaaatcgt caacgtgatg ggagaagccg
      361 gattcgcctg gtttgcccgc tgtgccgaac aggcaaggca aaaccagtac ttacaggtca
      421 gctcttgcgt gcccgctctg gaaggctgcg atgttaacgg tgccagtttt acccttgaac
      481 agatgctcgc ctggcgggac catccgcagg ttaccggcct tgcagaaatg atggactacc
      541 ctggcgtaat taacgggcag aatgcgctgc tcgataaact cgatgcattt cgctacctga
      601 cgctggacgg tcactgtccg ggtttgggtg gcaaagaact taacgcctat attgctgcgg
      661 gtattgaaaa ctgccacgaa agttatcagc tggaagaagg acgccggaaa ttacaactcg
      721 gcatgtcgtt gatgatccgc gaagggtccg ctgccagcaa tctcaacgca ctggcaccgt
      781 tgatcaacga atttaacagc ccgcaatgca tgctctgtac cgatgaccgt aacccgtggg
      841 agatcgccca tgaagggcat atcgatgcct taattcgccg cctgatcgaa caacacaatg
      901 tgccgctgca tgtggcatat cgcgtcgcca gctggtcgac ggcgcgccac tttggtctga
      961 atcacctcgg cttactggca cccggcaagc aggccgatat cgtcctgttg agcgatgcgc
     1021 gtaaggtcac ggtgcagcag gtactggtga aaggcgaacc gattgatgcg caaaccttac
     1081 aggcggaaga gtcggcgaga ctggcgctgt ctgccccgcc atatggcaat accattgccc
     1141 gccagccagt ttccgccagc gactttgccc tgcaattcac gcccggaaaa cgctatcggg
     1201 tcattgacgt catccataac gaattgatta cgcactcccg ctccagcgtc tacagcgaaa
     1261 atggttttga tcgcgatgat gtgtgcttta ttgccgtact tgagcgttac gggcaacggc
     1321 tggctccggc ctgtggtttg ctcggcggct ttggcctgaa tgaaggcgcg ctggctgcga
     1381 cagtcagcca tgacagccat aatattgtgg tgatcggtcg cagtgccgaa gagatggcgc
     1441 tggcggtcaa tcaggtgatt caggatggcg gcgggctgtg cgtggtacgt aacggccaag
     1501 tacaaagtca tctgccgtta cccattgccg ggctgatgag caccgacacg gcgcagtcgc
     1561 tggcggaaca aattgacgcc ttgaaagccg ccgcccgtga atgcggtccg ttacccgatg
     1621 agccgtttat tcagatggcg tttctttctc tgccagtgat ccccgcgcta aaactaacca
     1681 gtcaggggct atttgatggc gagaagtttg ccttcactac gctggaagtc acggaataat
     1741 aaaaaaagcc cggcgtcatg ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac
     1801 tgctgaattt ttttctcgcg gcggattttg cgttcttcca ttaccgccac tatcgccatc
     1861 aggcagatac aaccaatcgc ggcgatatcc agcgcggcga aggtgcctgc ccagccggta
     1921 aggccgaata ccggcgtccc atcggcaatc attcccagac ctaacttggc aaagctgtca
     1981 ccaatcaggt aagcaaaggt gcctttaata ccatcggcag cgccaatcgc ttttttaggt
     2041 acaaagccaa cagcagccac accaatcaac aattgcgggc caaagaccag gaaacccaac
     2101 gcaaagagag aagccagata gatatattcg ttactggcat gttgatacac accgagcgtg
     2161 gcgataatca gcgccagcgc gatgcaggcc accaggccac ggcgaccgtt cgccaggtca
     2221 gagagccagc cccacagcag cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc
     2281 tgaatcgcca ccgctttaga gagtttcagt tcctggaacg cgtatacggt tgaccactgg
     2341 tcgataccaa tacgtaccac atagaggaaa atgttggcga agcacagcag ccagatcact
     2401 ttgtttttca gcacatactc aacaaagatc tgccacttgg tcatatcggt agattctgtc
     2461 tctttgtcct cttcgctgat ctcctcgccg aacagttctt cagctttgcc gaggccataa
     2521 gattccgggg agtcgctgcc gtaacgcagg ccgataaaac cgacaatcag cgcgataatc
     2581 gacgggaaga taaacatgcc gatgacatgg ccatcgaaca ggtaatttgc cccgaacagc
     2641 gccacacctg ctgcgcctgc accgccaagg ttgtgagaaa tattccagaa accgaggaat
     2701 gtcccgcgtt tacgacgcgg cgtccatttg gtgatggtgg agtaactgca cgaaccgccg
     2761 gtactctgga aaaagccgct taaggcgtag aaggcaatca tcaggaacag gctaaccgag
     2821 ccgctgccca tactggcact gaagcccagc atacaaatgg cagagaggat aagcatgaac
     2881 ggcaggaatt gtttggtgtt tttgccgtcg gcgtagtatg aaaccaacgt tttacccacg
     2941 ccataagtga tggagaaacc caggccgatc atccccagct gcgtcatgct caacccgtag
     3001 gtcgaaatca tatcgttctg cgcgatgtta aagttcttgc gaatcaggta catcgtcagg
     3061 tagccgataa agaccaccag gtaggattgc atgaacggtt tgaaccacat tttgcgccgc
     3121 acttcgagcg gaaggtccag ggtcggcttg cgaacttggt ttaggaaagc cagcatgggt
     3181 tactcctgaa atgaatacct gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc
     3241 ctgagatagc gtccaagaaa aacctggaaa atttcttagt tttgccggat atgaagtgaa
     3301 aaggtgagat gcatcacgct tcacgcggtg tctgggcgtt caaaaagggc agtaacagca
     3361 gtgcggaaat cccggcggcg atagagataa ccacaaaaaa tccgctccag tgccaggtat
     3421 cgagcacttt cgccagcggc caaccagcaa gcgacgcccc cagataagca aacaagccga
     3481 caaaccccgt cgccgcccct gccgcctctt tgtgggaaca ctctgccgcc gccataccaa
     3541 ttaacatctg cgggccaaag acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca
     3601 cgtagctggc aaatggcatc agccacagcg agccgactga aagcaaaatt ccggcggcga
     3661 aaatcaaatt catcggccca cggttgccgt taaacaattt gtccgagccc caaccggcta
     3721 ccagcgcacc gataaatccg cccagttcaa acatcgtcac tgccgtattc gccgtgacca
     3781 gatcgacgcc cagcgtctcg gacatataca aattgcccca gtcgttgatc gccgcccgga
     3841 ccacatagac cagcacatag caaaacgaaa gcagccagat atacggattc agcaacacat
     3901 atttggtgag gatctcttta cgcgtcaacc ctgccccttc ttgttgttga gcaatttcca
     3961 gcgcgtcgtg ccgccattca ccgaccgccg gtaaacctaa cgcctgcggg cgatcgcgta
     4021 gccgccagca gagaaaaatc cccacgacta tcgccataca accagcaatc atcatcccgg
     4081 cacgccagcc gtaatgcagc gcgctcgctg ccatcacaat gggaatgagt gcgccgccga
     4141 cgttatgcgc cgtgttccat aatgcccacc aaccgccgcg ctcggtacgt gaataccagg
     4201 ccgttaacag acgcgcacac accggtgaac cccagccctg gaaaaaggcg ttcagcaccc
     4261 agagcacggc aaacgcccat agcgacgtcg agaagccaaa cagaatgttc atgatgcccg
     4321 tggcgataag ccctatcccc ataaaataac gggcatttga gcgatcgctg acaatgccgg
     4381 agacaaactt cgacacgccg taagtaatgt aaaacagggt cgctaacagg ccgatatcgc
     4441 tacggctgag cacgccgtta gcaaggattt ctggtaccgc ggcgttaaaa cttttccgcg
     4501 tgaagtaaaa cagcgcgtaa cccagccaga tggtcagcag aatatgccga cgccagtagc
     4561 gataacgggc atcaatttca tgtttatcag tcattaatgg cgcatcggca ggcgctttca
     4621 gaaacggcaa catcatcgaa ccttagacat aacgttgagg tagagaaacg ctgacacgcg
     4681 tgccgtgcag acaggaaatg tgtaatgtgc cgcccagcgc cgttacgcgc tcgcgcattc
     4741 cggtgaggcc aaaaccttgt tgcccggaac ccggcggcaa accgctgcca tcgtcttcaa
     4801 tcaccagcat caaccgttca tcctgctgcc agccttgcag cgtaaccgcg ctggcatcgg
     4861 catgtttcac aatgttgttc agcccttcct ggcagacacg aaacagcgtc acgcgctggt
     4921 tttcgcttaa tgctgattca tcgatacgcc attcgagatg gctgacaatg ccgcgccctt
     4981 ccagctccat ttcccgcatc agtgagcgga tggcctgctc cagggtgaga tcatccagct
     5041 ggcgcggacg taaccgtcct aacaaacggc gcaccgcgtc gtaaacgccc agcgacagtt
     5101 gttcgatgag ctgcccgctc tgcttcacac tggcgttatc tgccgccagc cgctgaacaa
     5161 tgcccgcctg agtacgaata gcagtgatgg tctgaccgat atcgtcgtgc agctcacgcg
     5221 ccacgtcgcg gcgcacgctc tcttcggttt ctaacaaacg ttcagccaga tgctgattgc
     5281 gcgccagttc cttttgtagc gactggttaa gttcacgcaa ccgctggatg cctgcgccca
     5341 gtaacaaccc tgtcagactt tgcaccagca acgagagtaa taaatccacc agatgatcgc
     5401 gccaggtttg actggcgatc agcgcgatgg cgttcatcaa cgtcgcaatc agcgctcctt
     5461 gccagccata gtgccaggcc agcgcgataa tcggcagcgc cagacagaat ggcgtaaagc
     5521 gcgacagttc gtccggcaat cccaactgga gccagagact gataacaaac agcagcaagt
     5581 accagaccag atgccgcccg cgccagttga tgggctgaga aaccagtgac gggccgagcg
     5641 gcagccaggt gttattggcg agatagtgcc agaacaccag acatatcggg gccagcgtca
     5701 gaccgccagt taaagtcagc aacagcgcat tccacgactc tttgccgtgc caaagccagg
     5761 gcagcgactg caacaacgcc gccgccgtta acgccgcacc ctgtaacagc aaggtgcgcc
     5821 agtcacgctg atggcgatag cgcgagatca gcgctacggg cagtaacgtc agcaaactac
     5881 cgatcatcaa taacggaaaa tgggttaaac cgaccgcctg cgttagccag taaatcagca
     5941 gccactccgc acccagcaat acgggccagt atccgcgcgg gcattgcagc attagcccca
     6001 gacgcagacc aaacggaaat aacagcaccg ccatatcagg gcgctcaacc agatgcaggc
     6061 tgatactcca taggcaaaac catgcggcag agaagataaa aaagcaggca ataacggtaa
     6121 ttaagcggga gaacaacgtc ttcatcacca gccatcaaac atgcggcgcg ccagctctac
     6181 gtcgttactg acgcccagtt tttccatcag attggcgcga tggacgtgta ccgttttcgg
     6241 tgacaagccc agttcggcgg caatctcttt caccgccatt ccttgcgcca gtttttccgc
     6301 cacctggcgt tcacgtttgg tcagcgggtc ctgacgaccg gatgccagtt taatggcaat
     6361 atccggcgtc agataacagc cgcccgtggc aaccgtatgc accgcagcaa tgagttcatc
     6421 cgggctacag cgtttggaaa gaaagccgcg tgcccccgcg ttaagcgcct gctcaaccag
     6481 cgcaggactg tcgtgaacgg agagcataat cgtcgccata cctttcggca gctggcttag
     6541 cagctccaga ccggagatat cgggcatgga gatatcgcaa atacacacct gcacaccgcg
     6601 ccccggcagc cccgccagcg cctcgcgccc cgaaccaaac tcggcaacta cctgcaaatc
     6661 aggttccagc cccagcagct gcgcaaagcc ggagcggacg atgaggtgat cgtctataag
     6721 ggcaacggtg atcatggtct tgtcctggcg ggtaaaaaaa aacgcgctta ccttaacgat
     6781 aagcgcgatg ttgttcaagc cttgagcggt tactgaaaaa acaccgcgat cttgttaaac
     6841 atcgtcggat cggactgatt acgctgcact ttcacgacat cttccagctt agcgatttgg
     6901 cttatcatct gctccagacg ctggtcgtca ttgaccagta gccagatatg gcttttgtcg
     6961 ctgtcctgaa tcggcagaca aagaatgcct tcaacgttaa aagcgcggcg ggcaaaaagg
     7021 ccacaaacgt gggtcattac gcccggatgg ttgcgaacgg tgagctccag aattacgttg
     7081 ttatgagttg tgttttgcat ggcttattcc cccaccattt cagtattcgc cgcacctggc
     7141 ggcaccatcg ggtaaacttt ttcttcggca tcaatgcgca catggatcag cgccgggcca
     7201 gggcgattga tgatttcctg caatgcagcc tgcggatcgg cttcgttatt caaatcacag
     7261 gtttcgaggc cgaatccggc ggcaatctgc ataaagttga ttttgcccgg ataggtggcg
     7321 gcaaaaacgc cttgctcgta gaacagactc tgttgctgat gcaccagccc cagcgcttcg
     7381 ttgttcatca gaatgatttt gacatccagc tgattttcac tggcggtcgc catctcctga
     7441 atattcatca tcaggctgcc gtcgccggag aaacacaaca ctttgcgatc cgggttcgcc
     7501 agcgccgcgc caatcgccgc aggcaggcca aaacccatcg tgcccagccc accggaggtc
     7561 agccactggc gtgggcgatt gagcggataa gcttgcgcgg tccacatctg atgctgaccc
     7621 acatcggtgg tgataatcgc attgtcatcg acacaggcgg caacggcgtt gatcaggccg
     7681 taatggctta atggatcgca cgctttcggg attggacacg ggaattcgcg ctgcaaatcc
     7741 gctaccaact ggtgccactc tgcacgcggt tgcgcttcca cccgcgggat caattgcgcc
     7801 agcacgtcat caacatccgc ctgaatcgcc acatgcggct gcttgatttt acccagctct
     7861 gcgcgatcga tatcgacatg aataattttg gcattcggac agaactgctc ggttttgcca
     7921 atcgcccggt catcaaaacg cgcaccgagc acaatcaaca aatccgcctc ctgcaaaata
     7981 tagttggtac tgcgcacgcc gtgcatcccc agcataccca gcgacaacgg atgcgctttt
     8041 ggcagcatgc ccagcgccat taaagtcatg gtggtaggca gttgcgcttt ctccgccagt
     8101 tcacgcaccc gcgcaggcgc attgatcaca ccaccaccca gataaagcac cgggcgtttg
     8161 gcagcgttaa tcattgtagc tgcgtcacga atgctttctt cgctaaaggc gggtgcaacg
     8221 gctttttctg ccaccgcggg ctgagcttca atctcaaaaa ccgccgtttg tacatcctta
     8281 ggaatgtcta tccacaccgg gctagggcgg cctgattgcg caatgcggaa ggcatcgctc
     8341 atgacctgtg ggagctcttc gatatgtctg accagatagt tgtgtttggt gatggggata
     8401 gagatgccgt aggtgtccac ttcttggaag gcgtcggtgc cgatcatcga ggcgggaacc
     8461 tgaccagtga tgcaaatcag cgggatggag tccagccgcg catcagcaat ggcggtcacc
     8521 aggttagtcg cacccggccc gctacaggcc atacagaccg ccggtttacc gtcggtgcgc
     8581 gccattccct gagcgataaa ccccgcgccc tgttcatggc gagccagaat atggcggatt
     8641 tgcgtgcttt ggcttaaggc gtcgtaaaca ggcaggatag aaccgcccgg aatgcccgtc
     8701 acaatcttaa tgccctgctg ttccaggaaa tgaacgataa attctgcgcc ggtaaagcgc
     8761 ttacgcgtcg atgttgtgcc cgaacttgcc atgctccagt ccttttcttc tgggccgact
     8821 ttccgggagg tgcttaaacg aaaaaccccg cccggtttgc gccggcgggg ttttggaatc
     8881 gtgtgttgtt ccagtcccta cggcgcattg ccgacgacca ccaccacacg cacgacgacc
     8941 actgcggcgg atggcgcagt tggtagtagt tttgcgttga acatggaagt agtcattggg
     9001 gaccttgttg gttttgtgtt taacaatatt tatacaagca cagctttaca ggggagacaa
     9061 tggaaaattt ttcagcaagg gaaaattgag gggttgatca cgttttgtac tgaattgcag
     9121 ataacaaaaa accccgccgg agcgaggttt cgtcagccgc ctgcggctgg taaccgcaaa
     9181 gcacactgta ttatgtcaac actgaaagta tacgtgttcc gcgcagaacg cgcaatttcg
     9241 gcacgaattt tgacgtattt agtgcatagt tgagtatcgt tcacagtttg cgttttgtcc
     9301 aaatattact gtttatttat acagtaagct tctataatat cactgtacgc aatgtgttat
     9361 gcgggggccg catcgttacc cggcgcacta agtcctggct gaaacgggtg gtgccgtcag
     9421 cgccttaacc ccgcgtgagc acactgtgtt atgtcaacaa gcacaacgtt tctccttgag
     9481 ataccgcgtg cacaacagct ggcaacaggc agcggaaagg tacgtcagct ggcagtgctc
     9541 ctgaaccaca ggagacgcgt atgagcctgg tggatatcgc cattcttatc ctcaaactca
     9601 ttgttgcagc actgcaactg cttgatgctg ttctgaaata cctgaagtaa ttcagattca
     9661 agtcgcacca aaggggagcg ggtaaccgct ccccttttat atttagcgtg cgggttggag
     9721 tcggatgcga tgctgacgca tcttatccgc cctaccatcc ctcccggcaa catttattgc
     9781 cgcttttgtt tacatattct gccgctaaac aattccccat tcctggcgta tatctggcta
     9841 acattcatca atgtgataga ttcctctccc gcatttatgg gaatgcgtag tgacttattc
     9901 taattatttt tataaaagca tccgtgataa tgaaaaggca aagaaacgtc aatttgttat
     9961 tgatgttggt attactcgtg gccgtcggtc agatggcgca aaccatttat attccagcta
    10021 ttgccgatat ggcgcgcgat ctcaacgtcc gtgaaggggc ggtgcagagc gtaatgggcg
    10081 cttatctgct gacttacggt gtctcacagc tgttttatgg cccgatatcc gaccgtgtcg
    10141 gtcgccgacc agtgatcctc gtcggaatgt ccatttttat gctggcaacg ctggtcgcgg
    10201 tcacgacctc cagtttgacg gtattgattg ccgccagcgc gatgcagggg atgggcaccg
    10261 gcgttggcgg cgtaatggcg cgtactttgc cgcgtgattt atatgaacgg acacagttgc
    10321 gccatgctaa cagcctgtta aacatgggga ttctcgtcag tccgttgctc gcaccgctaa
    10381 tcggcggtct gctggatacg atgtggaact ggcgcgcctg ttatctcttt ttgttggttc
    10441 tttgtgctgg tgtgaccttc agtatggccc gctggatgcc ggaaacgcgt ccggtcgatg
    10501 caccgcgcac gcgcctgctt accagttata aaacgctttt cggtaacagc ggttttaact
    10561 gttatttgct gatgctgatt ggcggtctgg ccgggattgc tgcctttgaa gcctgctccg
    10621 gcgtgctgat gggcgcggtg ttagggctga gcagtatgac ggtcagtatt ttgtttattc
    10681 tgccgattcc ggcggcattt tttggggcat ggtttgccgg gcgtcctaat aaacgcttct
    10741 caacgttgat gtggcagtcg gttatctgct gcctgctggc tggcttgctg atgtggatcc
    10801 ccgactggtt tggcgtgatg aatgtctgga cgctgctcgt tcccgccgcg ctgttctttt
    10861 tcggtgccgg gatgctgttt ccgctggcga ccagcggcgc gatggagccg ttccccttcc
    10921 tggcgggcac ggctggtgcg ctggtcggcg gtctgcaaaa cattggttcc ggcgtgctgg
    10981 cgtcgctctc tgcgatgttg ccgcaaaccg gtcagggcag cctggggttg ttgatgacct
    11041 taatgggatt gttgatcgtg ctgtgctggc tgccgctggc gacgcggatg tcgcatcagg
    11101 ggcagcccgt ttaagcgcac gtcaccgcag catcgtcatc agctccatgg gagaacgatg
    11161 ctgctttatc agatcacgca tcacccgcat atgcggtgcg gagtaagaat aaaacgcctg
    11221 atagcccgca caaagcacgc tgtctggcgt ctcctgccga gcgtgcgggc acaggcgatg
    11281 ccagtcgcac gcctggcagt cacggcgcgg ttgcgggttt tcgctgccac accatttttg
    11341 taaggtggcg tcaaaaagct caatcgagat acgactgata tctgcccgca cccaacactc
    11401 aaacagggca attaaaaatt tcccccacag ctctgctgtc accgaagcgg ccgtcagatc
    11461 gccccggtca tcaaattcaa gatagggaat gagctgtacg gagcggatgc cccgctgttt
    11521 gaaattgtga taaaaatcgg caggttgcag agcatccttg tactctacaa ccagcttatg
    11581 cctgtcctct tcagcatcaa tgacctgact tcctgtcatg cgactctctc aataaatacg
    11641 ataagttgat gtatatgcgt tacagcaaac agtattgcga gagataacac cgcgagggag
    11701 atcaaaaatt tgtcacggac tacgtgaaat tgggaaaaat cgctattcgt gacatccatt
    11761 aaattgcgat tacgggtgta gtgccagagg atcagcgcca cgatggcgag gatgccaatc
    11821 gaaatccaga ataacatgcc cgcctggtgc cagttgtgtt tgatagccaa cgccatcaac
    11881 gcgccgtagc ccagcatggt acgaaaccac gccagtgatg tgcgctccgg ctgcagccct
    11941 gggtcggcaa tgcgtctggc tttacggcta tccggcatac aacaccagtc ccataacgat
    12001 caccgccaca accatcaata ttaagctgat gattaataag ctgttggtgt agggcaggtc
    12061 ctctttcaga cgcatcgctt tttcattgcg taaccagcgc aggtagccat acattgctaa
    12121 cccgccggaa aacagacaca acaacaacgc cagcagttca cgaatgacgg gcgtggcgaa
    12181 atctggcgca agttgatcaa gcccaacccc cgccgccaga aatcctagtg cggtacggat
    12241 ccacgccaga aaagtacgct cattagccag cgagaagcgg taatcgggcg cttctccaag
    12301 gcgggaaatt ttcattgttg ctcctttgca atttcactaa cgctctatct ttagcggttt
    12361 tttttaagaa tttcgtccgc ccgtttcctg atatgctttt gccatccgct acaaggaggt
    12421 cagatgggta tcatagcgca aaataaaatc agttcattgg gcatgctgtt cggtgccatc
    12481 gctttaatga tggggattat ccacttttca ttcggccctt tttccgcacc accgccaacg
    12541 cttgaaagca tcgtggcgga caaaacggct gaaattaaaa gaggtcttct cgccggaata
    12601 aaaggcgaaa aaattaccac tgtggagaaa aaagaggaca tggatatcga taaaatcctc
    12661 gatcaaagcg gtatcgcact ggccattgct gcgctacttt gcgcatttat tggcggtatg
    12721 cgtaaggaaa atcgctgggg cattcgcggc gcgctggttt ttggtatcgg catcgtgtgc
    12781 agcattttgc tgatattcct gattttttcc ttcctgaccg gcggttcgct ggtttaagca
    12841 gcctgaataa tgcccgccgg gtgatcatca gcgggcatta tcgtcagaat ttctgttttt
    12901 tctcctccac cttcactcct tgcgtcggta ggccggtgtc atagtcgcgt accactggcg
    12961 aatagccatc ttgtgggcgt gggcgaaacg cccccatcca gcgcggtcgt gcatctttac
    13021 gccacggacg cagactccat tggtaactgc ggaacggatc gcgaattttg tccatgtaat
    13081 ccaataaggc gtcatgcatt ttgctgcgaa cgtctgcaaa acggatatca tcgatcaggt
    13141 tatgcatttc atttgggtca ttacggcgat cgtaaagttc atcactggtg aagaggttga
    13201 gcaccagttt aaagtcatcc gtcacccagc aacgcaccgg aataaaaccg ccaaagctgt
    13261 catgctcaat ctcgtagcgg ttaaattcca ccatcacgcc gcgtggctct ttcacggcaa
    13321 ggatattttc ccccggcaga atctctggtt tttcaatatc tgccagcgcc atcattgtcg
    13381 gcagtaaatc aatatgactg actggcgtat cgacctgtcg ccgctcccct tgcggcgaac
    13441 ggatgatcag cggaatacgg gtgatgtcgt catacatcgc cgctccttta ctgatcagct
    13501 tatgtgcgcc catcatttca ccgtgatcgg aggtataaat gacccacgta ttttcacgtt
    13561 gctctggcgt taaggcattg atgacacgtc cgatttggtc atcaacaaag tcattgcagg
    13621 caaaatagag cggatggtga taaagcccgt catcaccgac tggcgatggc atcgcctgcg
    13681 cccataagcg gtgatgttcc ggtttgttcg ccaggtcatc ctgtgatttc tcgccaagtt
    13741 cgtagtaaaa atcagcgtat ttctctaaat actccaccgg acaggtgaac gggtgatgcg
    13801 gctcatcata cgaaaccacc atcaggaagg gttcctcggc gcgcacgggc tattgcagaa
    13861 aatctaccgc tcgattgctg atgcggtgcg cccaggtgaa ggtttcgtca atatggttcg
    13921 cctgtaaatc ttcgacgctg tttaggccat tgcgccacag gctgatctct ttttccgtca
    13981 gttcgctaag atagttcgcc ccatcaaacc agtaatcagc atcccactcc ggcggacact
    14041 cgccagtgcc gaaatagtca tgaccgtcga gatgccattt gccgatgtaa caggtgtgat
    14101 agccggcatc cttaaagtag cgccccatag tggagatgtt tttgcctggc gcgacgttgt
    14161 tggtccacgg gccggactgg ttagcgtaga taccggtgaa tagtccggcc cgtgcaggcg
    14221 tacaaaccgg tgaacaagtg taggcggaat taaagcgaat accttccgcc gccagactat
    14281 caatattttg cgtattcagc ggtttaccgc tatagcaacc gaccatattg gtggcctggg
    14341 tatcggtcaa ttggggcgtt tcataggcta tccttcgcgt cgggagtaaa ggttacggct
    14401 gggtcgtgac gatgccacca gctgtcaaaa atcaggtaga caatcactac ggcagcgatg
    14461 aaataactga tcatcgctaa ccagcgcgta ccgtagccgc cgaattcagc cagcccgaca
    14521 tagacgccaa tcatcgcgaa caggatacca attgacgcga tcttgacgtt tttccacggt
    14581 ttcatgtcca ccgcaaacgc atctttgaag gtgaacggcg tggcgcgcgg tttgataaaa
    14641 ccgatcacca gcatcacgac cacgttgatg cagaacgtac aggccagcac atagaggaaa
    14701 tggaagtcga acttcaccag atagttgatg gtgatgtagc tgattatgcc aatccccatc
    14761 gccacttttg ccgccagcgc cgggatgcgc ggaaagaaaa agcccataat gatgatggta
    14821 accagcggca cgttgtaaat gccgttgagc tgtttcatcc agctatacag cccctgcggc
    14881 gcgttcgcga tccacggcgc taccaaaacc gaaacgatag cgataaagaa accaaatttg
    14941 cgcccgacgg tgaccagctg ctgcggctcg gcattctggt taatgatgcg acggtaaata
    15001 cccatactga ataatgtact ggcgctattc agaaagccgt tgaaggtact gatcaccgca
    15061 ccaaataaca ccgcgccgaa gaaaccctcc agtggcactg gcagaacgtt attgaccagc
    15121 gtcgggtagg ccatgtcggc tttcggtaga tcctgataca gatgaaacgc aatcaaccct
    15181 ggcagcacca gtaccagcgg gtcgagcatt ttcagcaccg ccgttaacag cgcccccttt
    15241 tgcccttccg ccaggctttt tgacgccagc gtgcgttgca cgatgccctg atttgtacac
    15301 cagtaaaagg tgttcaccag aattaaaccg gtaaatgccg cgccaatcgg caagggatcg
    15361 gtcgggccac cgactgagtt taatttctcg gcgtgaacgg tggtaatttg ctcaatgccc
    15421 tgcataaagc tgcccttgcc tatcgcgatc aggccaaata ccggcaccat caacccgccg
    15481 ataaccagcc caataccgtt gatggagtcc gccactgcca ttgcgcgcag tccgccgatc
    15541 accgcataca aaattcccgc cagaccaagc aaaattacca gtagccagat agccgcaccg
    15601 tgagaaatct gtagcgattc cccgacgtga aacaggctgt tgagcgccaa cgcgccggag
    15661 tagagaacaa tcggcagaaa gcagacgccg gtggcaatga ggaagcagaa gtcgatgata
    15721 atacgcgtcg ttttatcata acgttcctcc agaaaatcgg ggatggtggc aatgccgcgc
    15781 ttcagatagc gcggtaaaaa gattagcgcg aggaagatca gcgtcaccgc tgaagtcact
    15841 tcccagccca tcaccgacat gccgcttttg taggcctgcc cggaaagacc aaccagttgt
    15901 tccgtggaaa ggttggttaa cattaacgaa gcggcaataa ccggcgcttt tagtgaacgt
    15961 ccggcaagaa aatagccttg ttgcgatccg gtatctgttt tgcgaacctt ccaccaggtg
    16021 atcaccgcca ccagcagcgt aaaaccgaca aaactcaaga tttgtaacga attcatctca
    16081 tagcccttat ttatgtttat gtcccggacg catgaatata gggagaggta aaatgcgtcg
    16141 ggtcaggcag tcagattctt aaaatacaaa cgtcgtatcc ctgaacggat tgttttctgt
    16201 taagttcagg ttgtgagcat aattcgccgg agggatatta aaatgaatgg aaaattgcaa
    16261 agttcagatg taaaaaacga aactccgtac aatattccct tattgattaa cgaaaatgtg
    16321 atctccagcg gaatttctct gatctcgctc tggcatacct acgccgacga gcattaccgg
    16381 gtgatctggc cgcgggacaa gaagaaaccg cttatcgcca actcatgggt tgcggtgtat
    16441 accgtacaag gatgtgggaa aattctttta aagaatggcg aacaaataac tctgcatagc
    16501 aactgtatta tatttttaaa gccaatggat attcactctt atcactgtga aggtttagtc
    16561 tgggaacagt actggatgga atttaccccc accagtatga tggatatttc cgttggtcag
    16621 caaagcgtta tttataatgg cgaaatttat aatcaggaac tcaccgaagt tgctgagtta
    16681 ataacttcac cagaagcaat aaaaaataat ctggcagtcg cttttctgac gaaaattatt
    16741 tatcagtgga tttgtcttat gtccgcagat ggtaaaaaag atccacaacg gcggcaaatt
    16801 gaaaaattaa ttgccacttt acatgccagt ctgcaacaac gctggagcgt agctgatatg
    16861 gctgccacga tcccctgtag cgaagcctgg ttgcgtcgtc tgtttttacg ctataccggc
    16921 aagacgccga aagaatatta cctcgatgcg cgtctggatc tggcgctatc gctattaaag
    16981 caacaaggaa actcggttgg cgaagtcgct gatacgctca acttcttcga ctcctttcat
    17041 ttcagcaaag cctttaaaca taaatttggt tatgcgccgt cagccgtgct aaagaatacg
    17101 gaccagcacc caacggatgc cagtccacac aattaacgta actctggcca gtacgctttg
    17161 ttggcctcca gcaactcatc cagaatagct ttggcgaccg aggcgctcgg taccgttttc
    17221 gacaacgtaa tcgcctgcca caggtgctga tatgaacgct gttcccaggc gtccacgacc
    17281 agtttttcca ccgccacttg ctgactcatt aaccctttct gaaactgcgg gatatcgccg
    17341 accaccagtg gttcaggccg tagtagcgca cgcgcatctg tttgcgatct tgcagcccaa
    17401 caatttgcgc catccggctt tcaataccga ttggcatgtc acagatgttg aggattttcg
    17461 cattcgggcg cagacgacgc gtggcttctg cgacaattgc tgccgggttg gagtagttga
    17521 gcatctaggc gtttggtgaa tatttttcca tataatccac cagttccagg acgccgccaa
    17581 tggaacgcat gccgtacgct attccgccgg gtccgcaagt ttcctgacca acaacaccgt
    17641 ggcgcagtgg gattttttca tccagttcgc gcatcgggta cttgccgacg cggatgtgcg
    17701 ccatgacaaa atcaacgtcg ctgaacgcca cttcaggatc ggtggtgtaa ctaaacgcaa
    17761 tgtccggcgc tttttcttta aggatgactt tacaggcttc ggcaatcact tcctgccgcg
    17821 cgccatcgtt atcataaaat ttcagtgcac gaagcgggaa acggtcctga ttcgccagga
    17881 gcatcaacac gatgcctggc gtaaaggtgc ttccaccgcc tgcgacaacc actgagaatt
    17941 tggtcattat actgcctccg taatggcaac attttctgct gattgatgag aattaattaa
    18001 gctatcgagc tgttcacgca attgggatac atgcagacca atgatcacct gaatggcatc
    18061 gccactacgg aagacgccgt gcgctcccag ctttttaaag acttcgtcat ccagcgtttg
    18121 tgacatgtca tgcagtgcaa tacgtagacg cgtcgcgcaa ttgttaatgc tggagatatt
    18181 gccgaccccg cccagggctt gcaggatacc ggcagcctga tccagctctt tttttggctc
    18241 tgcagcggtg gtttggcctc gcgaggcttt gtattcggct tttgagtaga gtttcacttc
    18301 cgcatcttca cgtcccggcg tgcacatgtt gaactgcaga atcagggtgc ggaaaaccac
    18361 gaagtacagc agggtaaagc acaacccaat ggcgatttgg gtcagcatca tatccgcgtg
    18421 gttgctgaac atcgggatcc agttttgcgg taaaacctgg tcaatcagac ctccgcccat
    18481 gttgcccacc acaccaaaga gatacattac ggtcgacatt gaggccgcca gcacggcgtg
    18541 taccgcaaac agcaacggtg aaatgaacag gaaggtaaat tccagcggtt cggtaattcc
    18601 caccagcatg gcggttaagg ttgcgggaat cagcaagccc gccactttta cccgattttc
    18661 cggtgcggca gtgaagtaca tcgctaaaga aatgcccacg gcaccaaaga tttttgagtt
    18721 accgtgcagg gcaaaaccgc cttccgggaa caacgatttc agtggctcgg cgctcagact
    18781 gaactcttgc agatgctgcg cccagtacat ctgaatgccg ccttcaacag ctgccggacc
    18841 aaagataaac ggtccgtaga tgaagtggtg taaaccggtt gggatcagaa tacgttcgag
    18901 gaaggtgtaa acccacaccc caagcgcacc cgccgaacgc aggaacgctt gcagagattc
    18961 aatccccatt tgtacttttg gccagccgag caatgtcagc caggcacagg ggatcatcac
    19021 caggaaggcg ataatcacca cataagacgt cccctggaaa atgccgagaa aaaccggcag
    19081 ttttttatcg aacagacggt tatgcagcgc cgtcacaatg ccggaaatga taattgcgcc
    19141 gataatgctg gtatcgaggg ttttaatccc ggccatcatt gtcagaccgc tacctgccac
    19201 cgcgtcctga gtgaaatcga cgccgaagta gcttccccag gtcattccca tcgcattgat
    19261 gaaatagttc caggtcagga aactcaccat caccgccaga caagcacgtc cctgcgcttg
    19321 cttagcaagg ccaatgggta aaccgacagc aaaaatcagc ggcatattac ggaataccgt
    19381 ccaaccgccc tcttcaataa tgtgtacgat ttgcgcgaat aaactgttcg gatcggtcag
    19441 tgattccccg acaaacatcg ggttttgcag caagatggca agacccacca caatcccggc
    19501 gaagggaaac agcagcactg gcgtgaacat cgcgccgcca aagcgttgaa tttgactgag
    19561 catttccaca tcctcttttc tcaattctgt aagtaggaag cctgattggt tatgcggcag
    19621 acctggagcg aggataggaa gatgcgatcg gcagaacatg acgactcaat gggattcgtg
    19681 atcgctttca tgcttttcgt tgggcggtag agatctacgt tttcgcgaaa aagtagacat
    19741 ctgtgagcac aggtacacag catcgaacac atctttaaaa aaaagatttt tttcaatcga
    19801 ttaagcagaa cttgtgggcg cattacccgt gcttgcaggc aaaaaagaga gctagagatg
    19861 atctacaaaa gcattgcgga gcggttaaga attcgactta attccgcgga tttcacgcta
    19921 aacagccttc ttcccggtga aaaaaagctg gcggaagagt ttgcggtatc gcggatgact
    19981 atccgtaaag ccattgacct gctggtagcg tgggggctgg tggtccgccg ccacggcagc
    20041 ggcacttacc tggtgcgcaa agatgtgctg catcaaaccg ccagcctgac cggactggtg
    20101 gaggtgttaa aacggcaggg aaaaacggtc accagccagg tgctgatttt tgaaatcatg
    20161 cctgcgcctc cggccattgc cagccagtta cggattcaaa tcaacgagca gatctacttc
    20221 tcccgtcgcg ttcgttttgt ggaagggaaa ccgctaatgc tggaagacag ctatatgccg
    20281 gtaaaactgt tccgtaacct ttcgctgcaa catctggaag ggtcgaagtt tgaatatatt
    20341 gaacaagagt gcgggatttt gattggcggt aattatgaaa gcctgatgcc ggtgctcgcc
    20401 gatagactgc tggcgcggca aatgaaggta gcggaacaca cgccactgct gcggatcacc
    20461 tcgttgtcat atagcgagag cggggagttt ttgaattatt cagtgatgtt cagaaatgcc
    20521 agcgaatact aggtggagta ccatttacgg cgactccacc cggaaaaaag ttaaccgata
    20581 ctccagaaga gcaccgccag taattggggg gtgataatgc gcaggaacat cactaacgga
    20641 tagacagtgg cgtaagagag cgccgccgca ccactggttg gatgaagatt attagcaaac
    20701 gccagcgccg gaggatcggt catggaacct gccagcatcc cgcacatggt caggtaattc
    20761 attttggcta acatccgcgc cagaatgcca acagtaatca gcggaacggc ggtgatcagg
    20821 gcaccataac caatccagct tagcccttcg ccattgacca gggtattcac aaaatcccca
    20881 ccagatttca gaccaacgac cgagaggaac agcacgatcc ccagctcccg cagcgcgagg
    20941 ttggcacttg gcggcataaa ccagtacagc ttgccgatac tgccgatacg cccgaggatc
    21001 aacgccataa tcagcggacc gcccgccagc cccagtttca acgcggccgg gaatcctggc
    21061 acaaagacgg gaatagaacc taacaatacg ccaagcccga tgccaataaa cactggcagc
    21121 atctgaacct gttgcagttt ttgctgcgca ttccccagca cattggcaac ggcatcaatt
    21181 gcggacggac gccccaccag gttgaggata tcgccgaact gcaggctgat atcgccactg
    21241 gcgaccagtt cgaccccggc acggttcagg cgcgagataa caacgtcata gcgttcttta
    21301 aagtgcaggt cgcgaatacg ttttccgagc acgttttcat tggtgaccac cacacgctcg
    21361 acgcgcaaat cagtgccttt cgtagacagc gaggtatcga cctcctgacc aatcaccagt
    21421 tgcgcattat gtaaatccgc tggctgcccc accagatgca gcaaatcgcc cagttggata
    21481 atggtatctg gcgaaggtac ttttagggtt tcttctcgtt tcagacgcga gcagataatt
    21541 ttgtcgccgt tgagaatcgg tacatcttta atggctaaat catgcaggtt agggttctca
    21601 acgcgaatat tgatagtctt gatcagcgcg ccgccattgg tgcgtgaaga ctcgtgctgc
    21661 tgagcttctg tctcgacatt gacgcggaaa ataacccgca acatccacat ggtgaacaaa
    21721 atcccgcaaa tgccgaatgg atacgccatc gcgtaactca tccccatctg atcgaccatt
    21781 tccattggtg tacccaggtc gcgcaagatc tgctgccctg cccccagcgc tggcgtattg
    21841 gttaccgcac cggagaaaat ccccagcact accggcagtg gaatatcaaa cagtttatgc
    21901 aggatggcgg taaccagacc accgatgatg acgatcagaa cagcaaacag gttgaggcgt
    21961 aatccggaga cgcgcaatga ggcaaagaag cccggcccta cctgaatccc gatggtataa
    22021 acgaacagga tcaggccaaa ttcctgaata acatgcagca tatcgctact taatgtcatc
    22081 cccgcctgag aaacaaaatg gccgacgatg atcccaccaa acagcacgcc gccaatacct
    22141 aatcctatgc cgcgaaattt gacgttgccg ataaacaaac cgacgactgc caccaaagcc
    22201 agaatactga ccgttaatgc tatatcactc atcgtccatt tctcttgaga taacatttta
    22261 attgctacgg attatggctg aatcggtgaa gcagatttgc cttatagcgc acattatcac
    22321 gggaaagaac gtgccgaaat atcttaaaca gtcgaaattt aacgcattgt tgaagaagaa
    22381 agacaaaaaa agccccacca gaatggcggg gcaaagagaa tagctagtta gctatttaac
    22441 gcgggacgtt cgctgatagc gatacgctgc gctgcgatgg gttcaggctc attacgaatt
    22501 aaatcaatat gcagtaaacc gtttacgaag gttgcgccag agacttccat attttcagcc
    22561 agcgtaaaac tcaggctaaa tggctgattc ataagccctt gatgcagcca ttttttctct
    22621 tcttttggct gctccggcgt gccttttacg ctcaggcgcg tgccttccag ttgaatctct
    22681 aaatcttcct gacggaaacc tgccagcgca agggtaatgc ggtagtggtt atcgtcgctt
    22741 ttctcaatgt tgtacggcgg gaagctctgg ctttcaccgg cgttttgcag tgcgttggcc
    22801 agtttgtcaa aaccgatcca ttgacgcatc agtggggata aatcgaagtt acgcatagtc
    22861 atttctcctt ctaagaagcg agtaagtacc tgcaaatccg aagattcgca tatgctccct
    22921 gacggcgagc atggagatgt caggccgcgc caggcggcct tagggaatta gttgatttcg
    22981 atacggcgcg gttttttcgc ttccggaatc acgcgttcga gatcgatata cagcaaacca
    23041 tttaccaggt tagccccacg aacatgaatg ttctcagcta actggaattt gcgttcaaag
    23101 ttgcgttcag cgatgccctg gtacagatag gtgcgctctt tttgttcgtc ggcgtgagca
    23161 cctttcacca ccagcagatt atcctgggcg gtaatttcca gttcgctctc agcaaaacca
    23221 gccacagcga tagcaatgcg gtaatggttt tcgtctacca gttcaacgtt atacggaggg
    23281 tagccgccat tactctggct ctggttgttt tctaagtggt taaacaaacg gtcaaatcca
    23341 atagcagaac ggtaaagcgg ggataaatca aagttacgca taatcaatag ctcctgaaat
    23401 cagcgagaat gtaagacctt ccacaatgga caggtcaggt agccagaaca cccatcaggc
    23461 gcgtcctcat cgactacgat gtaaaatggg tctggaaatg aacttttcaa gccttatcag
    23521 cggacttttt tttgcagttt atggtctatt gcatagactg agggggcagc acgaaacgtt
    23581 aaagtgcgat agccgccaca gtgcgacgta atgatgatgt tattttcagc aagggatcgc
    23641 tataactcat caggccaaca cccacgatca acagatgaat ttattatgat aagaaatgtg
    23701 ttgttagcgt tcatgatatg cagcggaatg acattactcg gaggatgctc cagcgtgatg
    23761 tcccataccg gggggaaaga aggaacgtat cccggcaccc gcgccagcgc caccatgatt
    23821 ggcgacgatg aaaccaactg gggcactaag tcgctggcga ttctcgatat gccgtttacc
    23881 gctgtaatgg acacgctttt actgccgtgg gatgtgttcc gcaaagacag ctcggtgcgc
    23941 tcgcgcgttg aaaaaagcga agccaacgcg caagccacca atgcggttat tccaccggcc
    24001 agaatgcctg ataactgatt tagcgatcag tttcggtgat ccacagctgg ccaccttcca
    24061 tccacgccag ccattgacca tccggcgaga agaccacggc gtccgcagaa ggtggattgg
    24121 cgtggttttc ggttaaatac tcaacctcgc cgctttgcgc atgggcacaa acaattcgat
    24181 tatccagcac aaagcccaac cattctcctg acggatgcca gttaaatgca gactgaatat
    24241 ccgttttgtt atgggttaac ttgcgcggtt cgccgccctg tggcgagata agccacagtt
    24301 gcacaatgcc gttatcatca cgcattaaaa acgcgatttg cgtaccctgc ggattacagc
    24361 gcacccagtg gcgcgggacg ttgactaacc ccggataagc ccgatgatgg gtaaaggtta
    24421 aacgtcgctg cacgacgcca cgcggtggcg cgggtagggt tgtttccgtt ccacttaacg
    24481 gcgcatcacc tgccgatttc cagctagctt catcttgcgg taactcaacg ataaatagct
    24541 ccggcacttt ctcgcccttt ggcgaaagtg tgtcgccaat aaacgccagc gcgtgatttc
    24601 ctacccatcc ttcttcataa gcacgattga tttcatcact gccaggctgc ggcgtgggcg
    24661 tggttttact caccagcaca caccagtggc taccgctgta ttcacgcgga tgttgctttt
    24721 gtacgttgac cgggccaaac ggtgcagcaa ccccgacgtt tcgcaaatcc agcgccggat
    24781 cgagttgatg cattacatgg tcgttatagg taaagctccc cctttcaccg ttcgggctaa
    24841 agacatgcac atggctgcca ccgcgcagcg ctcctggcgt atacggcgcg gtaatatcca
    24901 ttgcatcgag attgctcacc ttgccgcctt cagcaatgac tccgcgtcga tgatggaagt
    24961 cgtaatgcca tgtttcatca ggattttcag ggccgtgaat aaagacatat ttctctgact
    25021 tcggatgaac ggtcaccacg ccgacgtgtg cgccctgtga cgcgcgatag ataacctcga
    25081 cctcgcctgt atggatattc acacgctcaa tggtctcgcc ggtaaacgac gccccagaag
    25141 gacgcacgtc aaataccagc cactggctgt cgggcgtcca ggtattggta tttgtattgg
    25201 tattggtatt ggtgagcagg tgattacggg gagcaaaggt tatctgtttc atggcgcggt
    25261 cctgatacaa aacggttccc gccatcatac ttcacaagga cttcactttc agacaatagt
    25321 cttttgccat cgtgatgaat gtgagtgaaa agatggaaca tttcgacgtg gcgattattg
    25381 gcctcggccc ggcagggtcg gcgttggcac gaaagttagc cggcaaaatg caggtgatcg
    25441 cgctggataa aaagcaccag tgcggtactg aaggtttcag caaaccttgc ggcggtctgc
    25501 tggcaccgga cgcgcagcga tcttttattc gcgatggact gacgcttcct gtcgatgtga
    25561 tcgccaatcc acagattttc agcgtcaaaa ctgtcgacgt cgccgcatcg ctcacgcgta
    25621 actaccagcg aagctatatc aatattaatc gccatgcttt cgacttgtgg atgaaatcgc
    25681 tgatccccgc cagcgttgag gtttatcacg atagcctgtg ccgaaaaatc tggcgtgagg
    25741 atgataaatg gcatgtcatt tttcgtgcag acggctggga gcaacatatt accgcccgct
    25801 atctggtcgg tgccgatggc gcaaactcga tggtgcggcg acatctctac ccggatcatc
    25861 aaatccgtaa atatgtcgct atccagcagt ggttcgcaga gaaacatccg gtgccgttct
    25921 actcctgcat ctttgataat gcgataactg actgttactc atggagtatc agcaaagatg
    25981 gttattttat ctttggcggt gcctatccaa tgaaagacgg tcagacgcgt ttcacgacgc
    26041 tgaaagagaa aatgagcgcc tttcagttcc agtttggcaa agcggtgaaa agtgagaaat
    26101 gcacggtgtt gtttccctca cgctggcagg attttgtctg tggtaaggac aacgcctttc
    26161 tgattggtga agcggcggga tttatcagcg ccagctcgct ggaggggatt agctatgcgc
    26221 tggatagcgc agagattctg cgtgcggtgt tcctgaaaca gccggagaag agcaacgccg
    26281 cttactggcg cgccacacac aaactgcgtt taaaactctt cggcaagata gtaaaaagcc
    26341 gatgcctgac cgcaccggct ttaagaaagt ggattatgcg cagtggtgtg gcgcatattc
    26401 cacagttgaa agattatcca acgcgcttca catcacccac cagcaggatg taagagagcg
    26461 cgccaatcaa cgcgacggcg gagatataaa ccagtgcagg cgcgaaaccg taaccctgcg
    26521 ccaggtaccc caccaccagc ggaacggtga tgccgcccag accaccggcg aagttaaaca
    26581 cgccgccggt taaaccaatc aggcgcatcg gtgccagaga agagaccagc gaccaggtaa
    26641 tcgaagcaaa accgttgccg aagaatgcca gcgccatcag gcacataatc atcatcggat
    26701 cgttggtgta gttagcgccc ataatgcagg tggagatcag caagccgcag ataatcggcg
    26761 ttttacgcgc aaagcccagt gaaaagccct tacgtaccag cagatccgcg acccagccag
    26821 agagcaggac gccgacaaac gccgcgagga atggcaccgt ggtcataaag cctgctttca
    26881 gcgccgtgat tcctttttcc tgggttaaat agttcgggaa ccaggttaag aaaaaccaca
    26941 gtgcagaagc caccgcaaat tgcccaagat aaacgccgat cagtttacga tggaacacca
    27001 gtttccagtc tttggctgtt aacggctgac gcgcctcttt cttcaccggc gcatcaccat
    27061 ccaccagacc gccgccatca cgaatgtaat ccagttcagc tttgctgata cctttggtca
    27121 ggcgcggcgg ctgataaacc ttaaaccaaa tcagcgacca gataatgccg ataccaccag
    27181 tgacaatgaa cacccagtgc cagctcaaca tctcctgaat ccagatcagc agcggagtca
    27241 aaaacgccag accgacaaac tgaccagacg tataaaaacc aacggcggaa gcgcgttcat
    27301 gttccgggaa ccagctggtc accatccggt tattggtcgg gaaagcgggc gcttcgaaaa
    27361 taccggttat cgcacgcaga ccaattaatg acattaatcc cgtggcaaag ccttggaata
    27421 aagtcgccac tgaccaacca aatatcgcaa taaaataagt cacgcgagaa cctacgcgat
    27481 ctaaaaacca accgccgggg atctggcata gcgtataaag ccaggcgaag gccgaaaata
    27541 catagcccat ttccgctttc gtaatgccga actcttcctg aatatgggcg gaagccacgg
    27601 ccaggttagc gcggtcaaca taacaaatga ccaccgtaat aaagatcatc accagcgtca
    27661 gataacgccg acgccccggc tttgctgcat taacgggaat atccatagcg agctttctcc
    27721 agattttggg catagcgaag ccgctcacca tgccctgtaa tttacagagg gttatatttt
    27781 tgtattgctg ttttagtgcc cgatgagggg cttacgtggc aggaattacc actctgctac
    27841 gctgttatct tcatgacgcc agagcggatt acgccagtcc ggggcatttt tactgaactc
    27901 aataacttta gcttcgtcga tttccacacc taagcccggt ttcgttaacg gtttaaagaa
    27961 gccgccaacc atgctgaagt cttctttgtt tttcacaaag tcgagtaact ccgcgccttt
    28021 gttgtaatga atgcccatac tttgttactg aagtaccgcg ttataggaaa cgaagtcgat
    28081 atgcaggcaa gccgccagtg caatcggtcc gaacggacag tgcggcgcaa gggtcacatc
    28141 ataggcttct gccattccag caattttgta gcattcggta ataccgcctg catgggagag
    28201 atccggttgc agaatcgaaa taccgcctgc ctccagcacg cgtttaaaat cgaaacgtga
    28261 gaacatgcgc tcacccgccg ccagtggaat atgcgtttgt gccgccaatt tcgggtagta
    28321 ttcggcttgt tccgccagca ccggctcttc aataaacagc gggcgatacg gctccagttc
    28381 tttaatcagc actttcgcca ttggcgcgct gacgcgaccg tggaaatcaa gaccaaactc
    28441 aatctgattg ccaaaagctt cacgaatttg tgccacggtg ttgactgccg catctaccgc
    28501 gcgggagtta tcaattagcc ccagttcttc acaaccgttc agtttgaagg tatcgaagcc
    28561 gatttcgcgc agggttttaa tgccgtcgat aacatccgcc ggacgatcgc cgccgaccca
    28621 actgtaggct ttaattttgt cgcgaaccag gccgcccatc agttgccaga ctggcgcatt
    28681 cagcactttg cctttgatat cccataacgc ctggtcgatc ccggcgatgg cgctcatcag
    28741 gattggaccg ccacgataaa atccggcgcg atacatcact tgccataagt cgttgatgcg
    28801 cgaaggatcc tgaccaatca aatagtcacc cagctcgtga actgccgctt ccaccgtacg
    28861 ggcgcggcct tcaatcacgg gctcgcccca accgaccacg ccttcatcgg tttcaatttt
    28921 caggaacatc cagcggggag gtaaacgata cgtggtaatt ttggtgattt tcattgcact
    28981 gcctctcgat acgccttaac aaatgctgct gcctgctgcg cggtgcgttc tacggactgc
    29041 ccggcgcgat aaagatcgct gcctaagccc gccccaacac aacctgcgtc tatccactgc
    29101 gccaggtttt ctggcgtcac gccgccaacg gcaaagactg cgatgtccgg tggtaatacc
    29161 gcttttaacg ctttgatgta ttgcggacca aaagccgatg acggaaatat tttcagcgcc
    29221 tgcgcgcccg cttcgagcgc ggtaaaggct tcggtcgccg tcgcgcagcc ggggcaaacg
    29281 gtcatgccgt agcccaccgc gcggcggatc acttcactat ggatattggg cgtaacgatg
    29341 agctgacagc ccatcctggc gagcgcatcg acctgttcag gtttcagtac cgtacctgcg
    29401 ccaatcaacg ccttgtcgcc atacgcatca acgatggcgg gaatgctctg ctcccattgt
    29461 ggggaattca gcgggatttc aaccgcgtcg aacccggcgt caatcaccgc gccaacatgc
    29521 gccagcgcct cgtcgggcgt aatgccgcgc aaaatggcga tcagcgggag tttagtttgc
    29581 cactgcatga gcgatgctcc ttataccagc ctgaaatgcc gtgtcgcccg ccaccgccgc
    29641 cacgtcgcaa cccatcgcct gaaaggcttg ctggtagcgc gcggtcagcg atgttccggc
    29701 gacaagggtg atggcgtgtt gatgggtcac atagtcgcgc atactggcaa cctctgcgcc
    29761 aatcaacaaa ccagagagaa attcgctgac ctgttcgcgg ggaagtgttc ccagcacatg
    29821 cgaggcgcga acttcaaaaa gctgcggcaa tatggcgggc gcattaaggc cgcgctcaag
    29881 gccagccgcg aaggcatcgg cagagttttc ctgcggcggc aaacctgcgc caatcaatga
    29941 gtgatttaac agtaaatgat gtaattcacc ggtcatcacg gtgcgaaaat cgttgatttg
    30001 ctggctatcg gcctgcaccc atttgcaatg cgtaccgggc ataacataaa gagaggaagg
    30061 agccagagct cgcgcgccga tcaattgtgt ttcttcgccg cgcatcacat tgtggttatc
    30121 gtcatgagag acgcataatc cgggaataat ccagatattg tcgccaacag acgttaattg
    30181 ttcgccaata gacgaaaaac gggcaggaac agataaatac ggagcaactt tccagccgac
    30241 attgctgccg accattcctg ccatcaccac tggcgtattt tcttcacgcc agtcggtcgt
    30301 gacttctgct aacactgcag ccggagattt tccgttcagg cgcgtgacgc ctgcttctga
    30361 ttgcctgctc tccaggcagt ggtcgccctg ataaagccag gcgcgcagat tggtcgatcc
    30421 ccagtcaatt gcgatgtagc gagctgtcat gtgatttcct ttaaccttcg tgtcgagctg
    30481 gcaatcatgg taagcgccgc ctgctctgcc gcatcgccgt cctgatgccg tatcgcatcg
    30541 aacagcgcct tatgttcctg gagcgtttgc ggcatgttgg cctcatcgcc catccaggtt
    30601 cgttcaaaaa ccgcccgctg cagcgaactg atcgcaatgc taagttgctg taacaccggg
    30661 ttatgcaccg actgcagcac cgcctcgtgg tagcgaatat ccgcttcgtt aaacgcttcg
    30721 cggtcctgat tgttggcaat catctcgttc agcgccgatt caatctgcgc cagatcgctg
    30781 gaggtcgcgc gctctgctgc ccaacgggca atcgccggtt ccaccagatt tcgcacttcg
    30841 ctcatggcac tgataagccg tgggtcgtag tcgttttcca gcacccattg cagtacgtca
    30901 gtgtcgaggt aattccactg gttacgcggt gccacaaacg ccccgcgata acgtttcatt
    30961 tcaatcagcc gcttcgccat cagcgaacgg aacacctcac ggatgatgtt gcgcgaggtt
    31021 gcaaactctt cacagagctc cgcctcagcc gcaagtggcg agccaggtac gtatttgccg
    31081 tgaacgatct gtttacccag cgtaatgaca atgcgatcgg ttttattgag agtcatggag
    31141 agtccttgtg ctctgtatgt tcttctctac tttaccccga tcgatgcata acgcggcaac
    31201 tttgtagtac cagcgtgatg acgttcgcgt ttgccgcgcg cgtaatgtag tacaaactta
    31261 tattgttgcg ctacaattta gatcaaaaaa agaacaacac ataaaaaaat gacatgcgtc
    31321 gggcagaaat ctgaaaaggg atatcaggcg ctaaacagga gggaaagaag agtatgcttt
    31381 caacggctta gctactcgtt taaaggatta atcatgaagt tgaattttaa gggatttttt
    31441 aaggctgccg gtttattccc actggcgctg atgctttcag gctgtatctc gtatgctctg
    31501 gtttcccata ccgcaaaggg tagttcagga aagtatcaat cgcagtcaga caccatcact
    31561 gggctatcgc aggcaaaaga tagtaatgga acaaaaggct atgtttttgt aggggaatcg
    31621 ctggattacc ttatcactga tggtgccgat gacatcgtta agatgctcaa tgatccagca
    31681 cttaaccggc acaatattca ggttgccgat gacgcaagat ttgttttaaa tgcggggaaa
    31741 aagaaattta ccggcacaat atcgctttac tactactgga ataacgaaga agaaaaggca
    31801 ctggcaacgc attatggttt tgcctgtggt gttcaacact gtaccaggtc actggaaaac
    31861 ctaaaaggca caatccatga gaaaaataaa aacatggatt actcaaaggt gatggcgttc
    31921 taccatccat ttaaagtgcg attttatgaa tactattcac ccagaggcat tccggatggt
    31981 gtttccgcag cattactgcc agtgactgtt acactggaca tcattactgc accgctgcaa
    32041 tttctggttg tatatgcagt aaaccaataa tcagtaagcg ggcaaacgcg tttatgctgt
    32101 ttgcccgccc acagattaat tcagcacata cttctcaata gcaaacgcca cgccatcttc
    32161 aaggttggat tttgtgacaa agttcgccac ttctttcacc gaaggaatag cgttatccat
    32221 cgccacaccg acgcctgcat attcaatcat tgcgatgtcg ttttcctgat cgccaatcgc
    32281 catgatttct tccggtttaa tacctaacac gtcggccagt gatttcacac ccgtaccttt
    32341 gttaacgcgt ttatcgagga tttcgaggaa gtacggcgca cttttcagca cggtatattt
    32401 ctctttcact tcctgcggaa tacgcgcgat agcctggtcg aggatggcgg gttcatcaat
    32461 catcatcact ttcaggaact gggtattggg gtccattttc tccgcttcgc agaacaccag
    32521 cggaattgtg gcaacgaagg attcatgcac cgtgtagtag ctgatatcgc ggttagcggt
    32581 gtacagcgtg gtgcggtcca gggcgtggaa atgagaaccg acttcgcgag agagtttttc
    32641 caggaaacga tagtcgtcat agctgagagc ggtttgcgcc acggtgctac catcagcggc
    32701 cttctgtacc agcgcgccgt tataagtaat gcagtagtcg cccggctgtt ccatatgcag
    32761 ctctttcagg tagttgtgca cacctgcata cggacgaccc gtcgttagca cgacattcac
    32821 gccacgggcg cgagctgcgg caatcgcatt tttaacggcg ggtgaaatgg tgtgatcggg
    32881 cagcagaagg gtgccatcca tatcgatagc aatgagtttg atagccatga gttcccaagg
    32941 tagattagtt cctgacccat gctaacgcga ttccgctcaa aaatcagtac aacacccgag
    33001 ggaaaagggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa gagtttccct
    33061 ttcagcagtt ccatgcctgc ggaaagcaga tcgttattgg cttgtgctga cacttcacct
    33121 tgcggtgaga gcgcatcaat aatcttcggc aattgctctg ccagtaaact ggaagccgta
    33181 ctggtatcca cgccaagttt ttgcccgaga tcggacaccg catttgtgcc gagcgccgac
    33241 tccagttgct cgccgctaac tgattgattt ctctgttgat tactcaacca ggttgagaga
    33301 atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc accctgctcc
    33361 tcaacccaac ttaaaatcgc ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca
    33421 acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt
    33481 atagatctgc gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg
    33541 cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttacccggc ctacaaaatc
    33601 atgcaaaatc aatatattgc aggagtctcg taggactaat aagcgtagcg catcaggcac
    33661 gctcgcatgg ttaacgccat taaatatcaa tattcgccgc tttcagggcg ttctcttcaa
    33721 taaacgcacg acgcggttct acggcgtcgc ccatcagcgt ggtgaacaac tggtcagcgg
    33781 caatcgcatc cttaacggta acgcgcagca tacgacggct ttccgggtcc atggtggttt
    33841 cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg gagaggccac
    33901 gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc ggctgacgac
    33961 gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc tcacccagcg
    34021 tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga tagtcagtat
    34081 ccacaccgtg ggtacgcacg cgaacaatcg gctcgaacag gttttgttcg gcattggtgt
    34141 ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg
    34201 cgttcaccca gcgggtaacg gtctgctcat cagaaaggtc agcttccgtc aacgtcggct
    34261 gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg ttgatcattt
    34321 tctgcgtcgc gttgtactca gataccagtt tctctaacgc ttcgccagcc aatgccggtg
    34381 cactggcgtt ggtgtgcagc gttgcgccgt ccagcgcgat agagatctgg tactgatcca
    34441 tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg tacagcggcg
    34501 gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga tagaagaagg
    34561 tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc
    34621 tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacag ccaagcgcgg
    34681 tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct ttctcgacgt
    34741 taaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc ccctgcttcg
    34801 cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt
    34861 cctggcagtc tgccagtttg cccggcaggc cagctaagtc gagcgcacct ttacggcggg
    34921 tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata attttgccaa
    34981 ccacgatttt cgcatcagtt gggttttcca gcaggtattc cgccagcagt tcgttcatct
    35041 gctgttcaac cgccgatttc acctcagaag aaaccagttt gtctttggtc tgggaggaga
    35101 atttcgggtc cggcactttt acggaaacga ccgcaatcag gccttcacgc gcatcgtcac
    35161 cggtggcgct gactttggct tttttgctgt agccttcttt gtccatgtag gcattcaggg
    35221 tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt
    35281 tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcagc gccacttcaa
    35341 cgccaatacc gtctttttcg gtggagaagt agaagatatt tgggtggatc ggcgttttgt
    35401 tcttgttcag atattcaacg aacgctttga tgccgccttc atagtggaag tggtcttctt
    35461 tgccgtcacg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac gacaactcac
    35521 gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag gtttcgaggc
    35581 tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc gccagcggag
    35641 cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc tgaataacca
    35701 gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg
    35761 acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc atgatcactt
    35821 ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct
    35881 gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag tgacccgcga
    35941 gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga ccggtgccgt
    36001 catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc cctttcagga
    36061 ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca tttatacttg
    36121 ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc gtccgacatg
    36181 tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt cgcttttaag
    36241 cggctggcaa gcaacccgcg acgctcatca tcaagctcag aggcaaaatc atctatcagg
    36301 tagagacacc gccgcccgct ttcacgagtg aggaactctc cttgcgccag acgtaaggcg
    36361 cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc accgtcggcg
    36421 cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt
    36481 tcaaaattac gttccagcac ctcagcgtac tctgtctctt tctcccagcc gcgctggaaa
    36541 gagaaagtca gagaaaactc agggagaaat tgcttacagg tatcatccat atcggccgcg
    36601 ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg gatcagttct
    36661 ttatcccacg ggcgtagctg ttcgtaacga gtcacctggc gcagcgccgc attgcgctgc
    36721 ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc caggttcgtt gtgaaagcat
    36781 ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt aaacccttct
    36841 ggcgttatca actgcatcgg catcaggtgc gccagttccg cgactttatg cccgtccgta
    36901 ccgtcaatac ggactttgct gtcgccctgt ttgtctttgg ttaagccaat cgccgtctcg
    36961 cgctcttcgc cctgtaatcg cccgtggaga acaaatgcct cctgctcatg gcgaatgacg
    37021 cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat ggcttccagc
    37081 acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg agataaagcg
    37141 agatccgcgg tttcaatatt gcggaaatcg cggatcaaca agcgggtgag ggacattaca
    37201 gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca atctgcacgc
    37261 tggaaaccga atcggtcagc atcatgcgga cgttttcgca tttcagcgcg ttcagaacat
    37321 ccagcacata gctgacgttg aagccgattt ccatctccgc accgctatag gtaacgtcga
    37381 ggatctcttc cgcttcttcc tgttccgggt tgttggcagt aattttcagc tggttttcgc
    37441 tgacatacag gcgcacaccg cggaatttct cgttagagag aatcgccgcg cgagcaaacg
    37501 cctgcttgag cagatcgcag cctgcttcca gatgtttgtc cgggttcttc ggcagaacgc
    37561 gacggtaatc cgggaagcga ccatccacca gtttcgaggt gaaaataaag tcgccaacgt
    37621 gggcacgaat gttattgctg ccaatctgca cacgcagcag attgtcgccg ccgtcgagca
    37681 tacgcatcag ttcaatcacg cctttacgcg gtacgatcac cgaatggctt ggcaaagatt
    37741 gaccaattgg cattgaacag accgccagac ggtggccgtc ggttgccacg gtgcgcagtt
    37801 cttcaccttc ggtttcaaac agcataccgt ttaagtaata gcgaacatcc tgatgcgcca
    37861 tcgaaaactg ggttgcttca atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga
    37921 cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta gacagcgaaa
    37981 aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgcacg gcaatttccg
    38041 cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc
    38101 ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg agatcagtac
    38161 cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga atcggtagcg
    38221 taggacgacc acctaacgga ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt
    38281 ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga aaaatcttct
    38341 ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc
    38401 gtgtggtcac gcccaccaaa cgcatcgcca atctctggca gactgtggtt agtcagctct
    38461 ttcgctagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa
    38521 aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat attgtcgatg
    38581 gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagcg cctcacgcac gaagtcgatg
    38641 gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc cccttccagc
    38701 tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcacc cggcaaacga
    38761 atgtcatttt cgtcggcctt tttcatcagg atcgccacac gggtttccag ctctggcggt
    38821 tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc aacgccgttg
    38881 atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc cagcagggcg
    38941 ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg
    39001 tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat cgcgttgttt
    39061 tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac cactttggca
    39121 ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt tttacccaga
    39181 cccgtgccgc cataaaggaa caacgggtta taggcaccgc cagggttatc cgccacctgg
    39241 caagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt
    39301 ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt atcccaacct
    39361 gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg
    39421 acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa
    39481 cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat attattaagg
    39541 tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc cagcgtgtta
    39601 tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc tggtaactca
    39661 tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga ctccactcga
    39721 acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac gtcgaaaaga
    39781 ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat caagatcctg
    39841 caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc tgtttctcac
    39901 agattttccc tatttatcca caggactttc cagaactcgc taagtgtaaa cgatcctgcc
    39961 gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag acaaaaattg
    40021 gcttaatcga tctaataaag atccaggacg atccttgcgc tttacccatc agcccgtata
    40081 atcctccacc cggcgcgcca tgctggtttc cactggtgtg aggtcgtaca ttttccctgc
    40141 gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccggt gtgtacaatt
    40201 attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc atttttcggc
    40261 ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc atgaaacgca
    40321 cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct cgtatggcta
    40381 ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg
    40441 tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag ggagttacgc
    40501 ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc tggcacgccg
    40561 caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg tcttacagtc
    40621 gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgcct gacgcgtgaa
    40681 agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa
    40741 ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc
    40801 cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa cgcctgatta
    40861 gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac ggaattgagg
    40921 cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc gtattaaaat
    40981 gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt gataccagag
    41041 aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt
    41101 catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc aacagaccac
    41161 gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca
    41221 ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca cccgtggtgg
    41281 tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta cccagccgtt
    41341 ccagctgttg gaaacttcac cgcagtttat ttatcaggca cagagcggtc tgaccggtcg
    41401 tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg aaaaagacgc
    41461 ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata ccgacgcggc
    41521 aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa
    41581 ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tcctcgtttg gtcagttgaa
    41641 gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg cactgcacac
    41701 cttccgtggc gcggcgtact ccacgcctga cgagaagtat gagaaataca agttcgatac
    41761 cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg cgatgctgca
    41821 acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact tctataccgc
    41881 taatctgggt aacggcatcg tcgctatcgg ctataaatct cagccggtac tggttcagcc
    41941 tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc aggacaaaat
    42001 ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt ggttcatctc
    42061 tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact ggggcttctc
    42121 cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca aagcgcagta
    42181 cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc gtgagcgtct
    42241 gggcgatgac aaacagcgta tcagtcagga aatgatggcg ctgtacaaag ctgagaaggt
    42301 taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc tggcgttgta
    42361 ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt ggatccacga
    42421 cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa cgatgttctt
    42481 cattcagaag atgtcgccga ccactgtgac cgacccgatg cagcagaaga tcatgacctt
    42541 tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg tactgtacta
    42601 tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg gtctggaaaa
    42661 acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt cgctaaaata
    42721 agggcggtca gttgaccgcc ttttttcttt tcgtagggcg gataagcacc gcgcatccgc
    42781 cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc aggccacgcc
    42841 tccgggacgt ggcggcgttg gcatcctgcg catctccggc ttcaaagccc gtgaagttgc
    42901 cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga
    42961 cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc cgaactcgtt
    43021 caccggcgaa gatgtgctgg aactgcaagg tcatggcggt ccggtgatcc tcgacctgct
    43081 gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg agttttccga
    43141 acgcgcgttt cttaacgata aacttgactt agcccaggcc gaggcgattg ccgatcttat
    43201 cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag gcgcattctc
    43261 cgcacgggtt aatcatctgg tagaagccct cacccacttg cgcatttacg tcgaagcggc
    43321 aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg aagcccagct
    43381 taatgacgtg atggccgatc tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt
    43441 gcgcgaaggg atgaaagtag tgattgccgg acgtcctaat gccggtaaat cgagcctgtt
    43501 aaacgcgctg gcgggccgtg aagcggcaat cgtaaccgat atcgccggaa ccacgcgtga
    43561 cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg acactgccgg
    43621 gctacgtgag gccagtgacg aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat
    43681 tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acgacagacg ccgttgatcc
    43741 ggcagagatc tggccggaat ttatcgcccg tctgcccgcg aaactgccga tcaccgtggt
    43801 acgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga acggtcacgc
    43861 gttaattcgt ctctcggcaa ggactggtga aggcgtggac gtgctgcgta accatctcaa
    43921 acagagcatg ggctttgaca ccaatatgga aggcggcttc ctggcgcgcc gtcgccactt
    43981 acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac tgttgggagc
    44041 ctgggcaggt gaactgctgg cggaagagtt gcgcctggca cagcagaact taagcgaaat
    44101 caccggggaa tttacttcag acgacctgct ggggcggatt ttctccagct tctgtattgg
    44161 taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg cttcattgtt
    44221 agcactcctg ttatgcctca accctttttt aaacattaaa attcttacgt aatttataat
    44281 ctttaaaaaa agcatttaat actgctcccc gaacgattgt gattcgattc acatttaaac
    44341 aatttcagaa tagacaaaa
//
LOCUS       DAZXUL010000023        44062 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZXUL010000023 DAZXUL010000000
VERSION     DAZXUL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44062
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.023"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            575..1024
                     /gene="alaE"
                     /locus_tag="ACH3L1_001168"
     CDS             575..1024
                     /gene="alaE"
                     /locus_tag="ACH3L1_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFT4075992.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(1061..1405)
                     /locus_tag="ACH3L1_001169"
     CDS             complement(1061..1405)
                     /locus_tag="ACH3L1_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFT4075993.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1557..1886
                     /locus_tag="ACH3L1_001170"
                     /pseudo
     CDS             1557..1886
                     /locus_tag="ACH3L1_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            2134..2379
                     /gene="nrdH"
                     /locus_tag="ACH3L1_001171"
     CDS             2134..2379
                     /gene="nrdH"
                     /locus_tag="ACH3L1_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFT4075994.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2376..2786
                     /gene="nrdI"
                     /locus_tag="ACH3L1_001172"
     CDS             2376..2786
                     /gene="nrdI"
                     /locus_tag="ACH3L1_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFT4075995.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2759..4903
                     /gene="nrdE"
                     /locus_tag="ACH3L1_001173"
     CDS             2759..4903
                     /gene="nrdE"
                     /locus_tag="ACH3L1_001173"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFT4075996.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4913..5872
                     /gene="nrdF"
                     /locus_tag="ACH3L1_001174"
     CDS             4913..5872
                     /gene="nrdF"
                     /locus_tag="ACH3L1_001174"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFT4075997.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6227..7429
                     /gene="proV"
                     /locus_tag="ACH3L1_001175"
     CDS             6227..7429
                     /gene="proV"
                     /locus_tag="ACH3L1_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFT4075998.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7422..8486
                     /gene="proW"
                     /locus_tag="ACH3L1_001176"
     CDS             7422..8486
                     /gene="proW"
                     /locus_tag="ACH3L1_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFT4075999.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8544..9536
                     /gene="proX"
                     /locus_tag="ACH3L1_001177"
     CDS             8544..9536
                     /gene="proX"
                     /locus_tag="ACH3L1_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFT4076000.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9728..10907
                     /locus_tag="ACH3L1_001178"
                     /pseudo
     CDS             9728..10907
                     /locus_tag="ACH3L1_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11031..11768
                     /gene="ygaZ"
                     /locus_tag="ACH3L1_001179"
     CDS             11031..11768
                     /gene="ygaZ"
                     /locus_tag="ACH3L1_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFT4076001.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11758..12093
                     /gene="ygaH"
                     /locus_tag="ACH3L1_001180"
     CDS             11758..12093
                     /gene="ygaH"
                     /locus_tag="ACH3L1_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFT4076002.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12184..12714
                     /gene="emrR"
                     /locus_tag="ACH3L1_001181"
     CDS             12184..12714
                     /gene="emrR"
                     /locus_tag="ACH3L1_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFT4076003.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12841..14013
                     /gene="emrA"
                     /locus_tag="ACH3L1_001182"
                     /pseudo
     CDS             12841..14013
                     /gene="emrA"
                     /locus_tag="ACH3L1_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            14030..15568
                     /gene="emrB"
                     /locus_tag="ACH3L1_001183"
     CDS             14030..15568
                     /gene="emrB"
                     /locus_tag="ACH3L1_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFT4076004.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15632..16147)
                     /gene="luxS"
                     /locus_tag="ACH3L1_001184"
     CDS             complement(15632..16147)
                     /gene="luxS"
                     /locus_tag="ACH3L1_001184"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFT4076005.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16297..17853)
                     /gene="gshA"
                     /locus_tag="ACH3L1_001185"
     CDS             complement(16297..17853)
                     /gene="gshA"
                     /locus_tag="ACH3L1_001185"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFT4076006.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(17926..18354)
                     /locus_tag="ACH3L1_001186"
     CDS             complement(17926..18354)
                     /locus_tag="ACH3L1_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFT4076007.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18351..18917)
                     /gene="yqaB"
                     /locus_tag="ACH3L1_001187"
     CDS             complement(18351..18917)
                     /gene="yqaB"
                     /locus_tag="ACH3L1_001187"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFT4076008.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(19065..19141)
                     /locus_tag="ACH3L1_001188"
     tRNA            complement(19065..19141)
                     /locus_tag="ACH3L1_001188"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19105..19107),aa:Arg,seq:acg)
     gene            complement(19206..19282)
                     /locus_tag="ACH3L1_001189"
     tRNA            complement(19206..19282)
                     /locus_tag="ACH3L1_001189"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19246..19248),aa:Arg,seq:acg)
     gene            complement(19347..19423)
                     /locus_tag="ACH3L1_001190"
     tRNA            complement(19347..19423)
                     /locus_tag="ACH3L1_001190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19387..19389),aa:Arg,seq:acg)
     gene            complement(19488..19564)
                     /locus_tag="ACH3L1_001191"
     tRNA            complement(19488..19564)
                     /locus_tag="ACH3L1_001191"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19528..19530),aa:Arg,seq:acg)
     gene            complement(19568..19660)
                     /locus_tag="ACH3L1_001192"
     tRNA            complement(19568..19660)
                     /locus_tag="ACH3L1_001192"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19624..19626),aa:Ser,seq:gct)
     gene            complement(19976..20161)
                     /gene="csrA"
                     /locus_tag="ACH3L1_001193"
     CDS             complement(19976..20161)
                     /gene="csrA"
                     /locus_tag="ACH3L1_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFT4076009.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(20396..23026)
                     /gene="alaS"
                     /locus_tag="ACH3L1_001194"
     CDS             complement(20396..23026)
                     /gene="alaS"
                     /locus_tag="ACH3L1_001194"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFT4076010.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(23154..23654)
                     /gene="recX"
                     /locus_tag="ACH3L1_001195"
     CDS             complement(23154..23654)
                     /gene="recX"
                     /locus_tag="ACH3L1_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFT4076011.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(23897..24958)
                     /gene="recA"
                     /locus_tag="ACH3L1_001196"
     CDS             complement(23897..24958)
                     /gene="recA"
                     /locus_tag="ACH3L1_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFT4076012.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(25038..25535)
                     /gene="pncC"
                     /locus_tag="ACH3L1_001197"
     CDS             complement(25038..25535)
                     /gene="pncC"
                     /locus_tag="ACH3L1_001197"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFT4076013.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(25680..26765)
                     /gene="mltB"
                     /locus_tag="ACH3L1_001198"
     CDS             complement(25680..26765)
                     /gene="mltB"
                     /locus_tag="ACH3L1_001198"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFT4076014.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSSGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            27021..27583
                     /gene="srlA"
                     /locus_tag="ACH3L1_001199"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             27021..27583
                     /gene="srlA"
                     /locus_tag="ACH3L1_001199"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            27580..28539
                     /gene="srlE"
                     /locus_tag="ACH3L1_001200"
                     /gene_synonym="gutE"
     CDS             27580..28539
                     /gene="srlE"
                     /locus_tag="ACH3L1_001200"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFT4076015.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            28550..28921
                     /gene="srlB"
                     /locus_tag="ACH3L1_001201"
                     /gene_synonym="gutB"
     CDS             28550..28921
                     /gene="srlB"
                     /locus_tag="ACH3L1_001201"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFT4076016.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            28925..29704
                     /gene="srlD"
                     /locus_tag="ACH3L1_001202"
     CDS             28925..29704
                     /gene="srlD"
                     /locus_tag="ACH3L1_001202"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFT4076017.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            29809..30168
                     /gene="gutM"
                     /locus_tag="ACH3L1_001203"
     CDS             29809..30168
                     /gene="gutM"
                     /locus_tag="ACH3L1_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFT4076018.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            30235..31008
                     /gene="srlR"
                     /locus_tag="ACH3L1_001204"
                     /gene_synonym="gutR"
     CDS             30235..31008
                     /gene="srlR"
                     /locus_tag="ACH3L1_001204"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFT4076019.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            31001..31966
                     /gene="gutQ"
                     /locus_tag="ACH3L1_001205"
     CDS             31001..31966
                     /gene="gutQ"
                     /locus_tag="ACH3L1_001205"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFT4076020.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31963..33477)
                     /gene="norR"
                     /locus_tag="ACH3L1_001206"
     CDS             complement(31963..33477)
                     /gene="norR"
                     /locus_tag="ACH3L1_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFT4076021.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            33664..35103
                     /gene="norV"
                     /locus_tag="ACH3L1_001207"
     CDS             33664..35103
                     /gene="norV"
                     /locus_tag="ACH3L1_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFT4076022.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            35100..36233
                     /gene="norW"
                     /locus_tag="ACH3L1_001208"
     CDS             35100..36233
                     /gene="norW"
                     /locus_tag="ACH3L1_001208"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFT4076023.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(36361..38613)
                     /gene="hypF"
                     /locus_tag="ACH3L1_001209"
     CDS             complement(36361..38613)
                     /gene="hypF"
                     /locus_tag="ACH3L1_001209"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFT4076024.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(38766..39293)
                     /gene="hydN"
                     /locus_tag="ACH3L1_001210"
     CDS             complement(38766..39293)
                     /gene="hydN"
                     /locus_tag="ACH3L1_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFT4076025.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(39442..40452)
                     /gene="ascG"
                     /locus_tag="ACH3L1_001211"
     CDS             complement(39442..40452)
                     /gene="ascG"
                     /locus_tag="ACH3L1_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFT4076026.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(40514..40585)
                     /locus_tag="ACH3L1_001212"
                     /pseudo
     CDS             complement(40514..40585)
                     /locus_tag="ACH3L1_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40595..42304)
                     /gene="hycE"
                     /locus_tag="ACH3L1_001213"
     CDS             complement(40595..42304)
                     /gene="hycE"
                     /locus_tag="ACH3L1_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFT4076027.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(42322..43245)
                     /gene="hycD"
                     /locus_tag="ACH3L1_001214"
     CDS             complement(42322..43245)
                     /gene="hycD"
                     /locus_tag="ACH3L1_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFT4076028.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(43248..>44062)
                     /locus_tag="ACH3L1_001215"
     CDS             complement(43248..>44062)
                     /locus_tag="ACH3L1_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFT4076029.1"
                     /translation="LFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAAL
                     PPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFL
                     GAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTV
                     SQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPV
                     KQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT        10475 a        11927 c        11193 g        10467 t
ORIGIN      
        1 tacagtcctg cgcgagatta tttcagctgt tattttctga aactctcaga ataatttcaa
       61 gattatatat tagccatttt gatgagtaat ttaggttatt ggtgaacaga gtggcgctct
      121 ctgctcgcta actccgcaag cctgacaaaa ccgaagtgaa taaaaatgca atataacaga
      181 ataaaagaaa acacccctca ctggtgcttt ctgctgttaa tgtacatttt ctggcatcaa
      241 aaatgctgta aatgacccgg tcatgacaaa caccgaattc tctacgctta tagcgttacc
      301 tcccccccaa actttcatta gtgaaattaa aggcaagatc atatctaatt gatttaattg
      361 aaaataaatc atattggcat gtaaaaatcc acaatataca aaaaacgagc cgttacggaa
      421 tattttatct acaaaaacta actaaataaa aatttttcac taattgatta gtcatagaca
      481 gcgatatacg ctatgcaaaa atgcagatgg caatgagatc cactgctttc atctccatta
      541 acatcccatt acgcttttat taaggagcat tagcatgttc tcaccgcagt cacgcttgcg
      601 tcatgcagtt gcagatacgt tcgcgatggt tgtttactgt tctgtcgtga acatgtgtat
      661 tgaagttttc ctctccggaa tgagcttcga acagtctttt tattccagat tggtagcgat
      721 tccggtgaac atcttaattg catggccata cggtatgtac cgcgatctgt ttatgcgcgc
      781 ggcacgcaaa gttagcccgt cgggctggat aaaaaatctg gctgatatcc tggcttatgt
      841 gacgttccag tcaccggtgt atgtggcgat cttgttagtg gtgggcgcag actggcatca
      901 gattatggcg gcggtcagtt caaacatcgt tgtttcgatg ttgatggggg cggtttatgg
      961 ctacttcctc gattattgcc gccgactgtt taaagtcagc cgttaccagc aggtaaaagc
     1021 ctgactcttc ttttcgcgac tggcatcgcc agtcgcgtct ttaactggct tcgccaaata
     1081 aaccattcaa ataacgttca agcgcaacac gcgaactaaa gccatgcggg ataccgtaat
     1141 gctcgtgtcg ctcgcctgct aaatagagcg gaaattgctg gtaatgatcg caagttttaa
     1201 tatcggaagc gggttcagcc agtgttgaac tgcgttcttt taatgttgga tgaattacca
     1261 gagcggtacg tcccattcgc gcttcgcgat taacatagac ataattttcc ccacggcggt
     1321 aaccatacgc tttctgggtt accacatcga cagtaaaacc gactttttca agtacgcgcg
     1381 ccacctcgtc tggtcgtaaa tacatatttg atcctcgtta ttttcaaccg cgggcctacc
     1441 ttacctgatt gcgcattcac aacgctttca gaaaagtcca taaagccgcg atgaacctca
     1501 gttaagaaaa ttatggtcta cactgaaaat tacatcgaat tctaatgggg gatcatatgt
     1561 ttaaccgacc gaaccgcaac gacgttgatg atggcgtgca ggatatccag aatgatgtca
     1621 atcaattagc tgacagcctc gaatctgtat tgaagtcctg gggcagcgac gccaaagggg
     1681 aagctgaagc cgcacgcagt aaagctcagg cgttgctgaa agaaaccagg gcgcgaatgc
     1741 atggtcgtac tcgcgtccag caagccgcgc gcgatgccgt tggctgagcg gattcttttg
     1801 ttcgtgaaag accctggtgt agcgtgggta cagcagctgc ggtaggtatt tttatcggcg
     1861 cactgttaag catgcgcaaa tcgtagtgca aaaatgataa taaatacgcg tctttgaccc
     1921 cgaagcctgt cttcggggtt tctttttgcc tggtgaatca caaaaatccc cctcccccgt
     1981 cacgctcata tccagggtaa tttcgatcac tatttgccat atattgtgta gttgaatctt
     2041 ttttcaacta catctagtat ctctgtatca acagagagac aacccgacgc gtatcatcgc
     2101 gccgtatctt cattttaaac ggaaatacga atcatgcgca taactattta tactcgtaac
     2161 gattgcgttc agtgccacgc caccaaacgg gcgatggaaa accggggctt tgattttgaa
     2221 atgattaatg tcgatcgcgt tcctgaagca gcggaagcgt tgcgtgctca gggctttcgt
     2281 cagttgccgg tagtgattgc tggcgatctt agctggtctg gtttccgtcc ggacatgatt
     2341 aaccgtctgc atccagcgcc acacgcggcc agtgcatgag ccagctcgtc tacttctcca
     2401 gcagctccga aaacacgcag cgttttatcg aacgtttagg tctgcccgcg gtgcgcatcc
     2461 cgctcaatga gcgggaacgg attcaggtag acgagcctta catcctgatc gtgccctctt
     2521 acggcggcgg cggtacggct ggcgcggtgc cacgacaggt aattcgcttt ttaaacgacg
     2581 agcacaaccg ggcgttgctt cgcggcgtta ttgcttctgg taatcgcaac tttggtgagg
     2641 cgtatggccg cgccggagat gtgattgccc ggaaatgcgg cgtgccgtgg ctgtaccgtt
     2701 ttgaactcat gggtacgcaa agcgatatcg aaaacgttcg taaaggagta accgaatttt
     2761 ggcaacgaca accgcagaat gcctgacgca ggaaacgatg gattaccacg cgctgaatgc
     2821 gatgcttaat ctttacgata gcgcaggtcg cattcagttc gataaagacc gccaggccgt
     2881 tgacgccttt attgcgacgc atgtgcgtcc gaacagtgtg accttcagta gccagcagca
     2941 gcgcctgaac tggctggtca acgaaggtta ctatgatgaa agcgttctta atcgctactc
     3001 tcgcgacttt gtcattacgc tgtttgccca cgcacacacc agcggttttc gtttccagac
     3061 attcctcggg gcatggaagt tttacaccag ctatacgttg aagacattcg acggtaaacg
     3121 ttatctggaa gattttgccg atcgagtcac gatggtggcg ctgacgctgg cacaaggcga
     3181 tgagacgctg gcgttgcaac tgaccgatga aatgctgtca ggacgctttc agccagccac
     3241 gccaacattc ctcaactgcg gtaagcagca gcgcggcgaa ctggtttcct gttttttgct
     3301 gcgtattgaa gacaatatgg agtcgattgg tcgggcggta aattccgcac tgcagctgtc
     3361 gaaacgcggc ggcggcgtag catttttgct gtcgaatctg cgggaagcgg gcgcgccaat
     3421 taaacgtatt gaaaatcaat cttctggcgt aattccggtg atgaaaatgt tggaagacgc
     3481 attttcctat gccaaccaac tcggcgcgcg tcagggggca ggtgcagtct atttacatgc
     3541 tcatcatccc gatattctgc gttttctcga cacgaaacgg gaaaatgccg acgaaaaaat
     3601 ccgcattaaa acactgtcgc tgggcgtggt gatcccggat atcactttcc atctggcaaa
     3661 agagaatgcg cagatggcgc tgttttcgcc ctatgacgtt gagcgagttt atggcaagcc
     3721 gtttgccgat atcgccatta gcgaacacta tgacgaactg gttgccgatg aacgcatccg
     3781 caaaaaatac ctcaacgccc gtgatttctt ccagcgactg gcagaaatcc agtttgagtc
     3841 cggctatccc tacatcatgt atgaagacac agtaaaccgt gctaacccta tcgccgggcg
     3901 cataaatatg agtaatctct gctcagaaat tttgcaggtt aacagcgcct cagagtatga
     3961 cgagaatctc gactatgccc gcacaggcca tgatatttcc tgcaatttag gttcgttgaa
     4021 tattgcgcac accatggatt cccccgattt ttcccgcacg gtagagactg ccgtgcgcgg
     4081 tttaacggcg gtgtcagata tgagtcatat ccgcagcgtg ccgtccatcg aagccggaaa
     4141 tgccgcctcg cacgccatcg gactggggca gatgaattta catggttatc tggcgaggga
     4201 aggcatcgct tatggttcgc cggaagccct ggatttcacc aatctctatt tctataccat
     4261 cacctggcac gcactgcgta cctcgatgtt gctggcgcgc gaacgcggtg aaaccttcgc
     4321 cgggttcaaa cagtcacgct atgccagtgg tgaatatttc agccaatatc tgcaaggtaa
     4381 ctggcagccg aaaacggcga aagttggcga actgtttgcc cgcagctgta ttacgttacc
     4441 tacccgtgag atgtgggcgc aactgcgcga cgacgtgatg cgctacggca tatacaacca
     4501 gaatctccag gcggtgccgc caaccgggtc tatctcttat atcaaccatg ctacgtcgag
     4561 tattcatccg attgtggcga aagtagagat acgcaaagag ggcaaaacag gacgcgttta
     4621 ctaccctgcc ccgtttatga ctaacgaaaa tctggcgctg tatcaggacg cttacgaaat
     4681 tggcgcagaa aagatcatcg acacctacgc ggaagcgact cgccatgtcg atcaggggct
     4741 gtcgctgacg ctgtttttcc ccgataccgc caccactcgc gatatcaaca aagcgcagat
     4801 ttacgcctgg cgcaaaggta tcaaaacgct ctattacatc cgcctgcgtc agatggcgct
     4861 ggaaggcact gaaattgaag gctgcgtctc ctgtgcactt taaggaatat ctatgaaact
     4921 ctcacgtatc agcgccatca actggaacaa gatatctgac gataaagatc tggaggtgtg
     4981 gaatcgcctg accagcaatt tctggctacc agaaaaggtg ccgctgtcga acgatattcc
     5041 cgcctggcag acattaactg tcgtagaaca acaactgacg atgcgcgttt ttactggcct
     5101 gacgctgctc gacacgctgc aaaatgttat cggcgcgcct tcgctgatgc ccgatgcgct
     5161 cacgcctcat gaagaggcgg tattatcgaa tatcagcttt atggaagcgg ttcatgcccg
     5221 ctcttacagt tcgattttct cgacgctgtg ccagaccaaa gatgtcgatg ccgcctacgc
     5281 ctggagtgaa gaaaacgcac cgttgcagcg aaaagctcag attattcagc aacattatcg
     5341 cggtgatgat ccgctgaaaa agaaaatcgc cagtgtgttt cttgaatctt ttttgttcta
     5401 ttccggtttc tggctgccga tgtatttttc cagccgcgga aagctgacca ataccgcgga
     5461 cctgatccgt ctgattatcc gcgatgaagc agtccacggt tactacatag gctataaata
     5521 tcagaaaaac atggaaaaga tatctctggg acaacgtgaa gagttgaaga gtttcgcctt
     5581 cgatttgttg ctggaactct acgacaacga gttgcaatac accgatgagc tgtacgccga
     5641 aaccccgtgg gctgacgatg tgaaagcgtt tctctgttac aacaccaata aggctttgat
     5701 gaatctgggc tacgaaccgt tatttcccgc agaaatggcg gaagtgaatc cggcaatcct
     5761 cgccgcgctt tcgccgaatg ccgatgaaaa tcacgatttc ttttccggtt caggctcctc
     5821 ttatgtgatg gggaaagcgg ttgaaacaga agatgaagac tggaatttct gagggtgtta
     5881 ttttcaaaaa tatcactacc cgcagcaggg aaataattcc cgccaaatag ctttttatca
     5941 cgcaaatatt ctgtagtgat ctacactgat actctgttgc attattcgcc tgaaactaca
     6001 atattcaggc gttttttcgc tatctatgac aaaaaatatc aacttttctc gatttgctct
     6061 cagcccttat atcacggaaa attccggcga tttgctcgca tcaatattca tgccacattt
     6121 gccctcaggg gttgcctcag attctcagta tgttagggta gaaaaaagtg actattccca
     6181 ttgggtaata tatcgacata aacaaataaa gaaatcattc tattgcatgg ccattaaatt
     6241 aaaaattaaa aatctttata aaatatttgg tgagcatcca cagcgagcgt tcaaatatat
     6301 cgaacaagga ctttcaaaag aacaaattct ggaaaaaact gggctatcgc ttggcgtaaa
     6361 agacgccagt ctggccattg aagaaggcga gatatttgtc atcatgggat tatccggctc
     6421 gggtaaatcc acaatggtac gccttctcaa tcgcctgatt gaacccaccc gcgggcaagt
     6481 gctgattgat ggtgtggata ttgccaaaat atccgacgcc gaactccgtg aggtgcgcag
     6541 aaaaaagatt gcgatggtct tccagtcctt tgccttaatg ccgcatatga ccgtgctgga
     6601 caatactgcg ttcggtatgg aattggccgg aattaatgcc gaagaacgcc gggaaaaagc
     6661 ccttgatgca ctgcgtcagg tcgggctgga aaattatgcc cacagctacc cggatgaact
     6721 ctctggcggg atgcgtcaac gtgtgggatt agcccgcgcg ttagcgatta atccggatat
     6781 attattaatg gacgaagcct tctcggcgct cgatccatta attcgcaccg agatgcagga
     6841 tgagctggta aaattacagg cgaaacatca gcgcaccatt gtctttattt cccacgatct
     6901 tgatgaagcc atgcgtattg gcgaccgaat tgccattatg caaaatggtg aagtggtaca
     6961 ggtcggcaca ccggatgaaa ttctcaataa tccggcgaat gattatgtcc gtaccttctt
     7021 ccgtggcgtt gatattagtc aggtgttcag tgcgaaagat attgcccgcc ggacaccgaa
     7081 tggcttaatt cgtaaaactc ctggcttcgg cccacgttcg gcactgaaat tattgcagga
     7141 tgaagatcgc gaatatggct acgttatcga acgcggtaat aagtttgtcg gcgcagtctc
     7201 catcgattcg cttaaaaccg cgttaacgca gcagcaaggt cttgatgcgg cgctgattga
     7261 tgcgccgtta gcagtcgatg cacaaacgcc tcttagcgag ttgctctctc atgtcggaca
     7321 ggcaccctgt gcggtgcccg tggtcgacga ggaccaacag tatgtcggca tcatttcgaa
     7381 aggaatgctg ctgcgcgctt tagatcgtga gggggtaaat aatggctgat caaaataatc
     7441 cgtgggatac cacgccagcg gcggacagtg ccgcgcaatc cgcagacgcc tggggtacac
     7501 cgacgactgc accggctgac ggcggtggtg ctgactggct gaccagtacg cctgcgccaa
     7561 acgtcgagca ttttaatatt ctcgatccgt tccataaaac gctgatcccg ctcgacagtt
     7621 gggtcactga agggatcgac tgggttgtta cccatttccg tcccgtcttc cagggcgtgc
     7681 gcgttccggt tgattatatc ctcaacggtt tccagcaatt gctgctgggt atgccagcgc
     7741 cagtggcgat tatcgttttc gctctcatca cctggcagat ttccggggtc ggaatgggcg
     7801 tggcgacgct ggtctcgctg attgccatcg gcgcaatcgg tgcctggtcg caggcaatgg
     7861 tgactctggc gctggtgtta actgccctgc tgttctgtat cgtcatcggt ttgccgttgg
     7921 ggatatggct ggcgagaagt ccgcgagcgg cgaaaattat tcgtccactg cttgatgcca
     7981 tgcagaccac gccagcgttt gtttatctgg tgccaatcgt catgctattt ggtatcggta
     8041 acgtgccggg cgtggtggtg acgatcatct ttgcgctgcc gccgattatc cgtctgacca
     8101 ttctggggat taaccaggtt ccggcggatc tgattgaagc ctcgcgctca ttcggtgcca
     8161 gcccgcgcca gatgctgttc aaagttcagt taccgctggc gatgccgacc attatggcgg
     8221 gcgttaacca gacgctgatg ctggcccttt ctatggtggt cattgcctcg atgattgccg
     8281 tcggcgggct gggtcagatg gtacttcgcg gtatcggtcg tctggatatg gggcttgcca
     8341 ccgttggcgg cgtcgggatt gtgatcctcg ccattatcct cgatcgcctg acgcaggccg
     8401 ttgggcgcga ctcacgcagt cgcggcaacc gtcgctggta caccactggc cctgttggtc
     8461 tgctgacccg cccattcatt aagtaactct gcacttgccc ggtgacgccg ggcattatca
     8521 ccctgccaaa aaaaggaata acaatgcgac atagcgtact ttttgcgaca gcgtttgcca
     8581 cgcttatctc tacacaaact tttgctgccg atctgccggg caaaggcatt actgttaatc
     8641 cagttcagag caccatcact gaagaaacct tccagacgct gctggtcagt cgtgcgctgg
     8701 agaaattagg ttataccgtc aacaaaccca gcgaagtgga ttacaacgtt ggctacacct
     8761 cgcttgcttc cggcgatgca accttcaccg ccgtgaactg gacgccactc catgacaaca
     8821 tgtacgaagc tgccggtggc gataagaaat tttatcgtga aggggtattt gttaacggcg
     8881 cagcacaggg ttacctgatc gataagaaaa ccgccgacca atacaaaatc accaacatcg
     8941 cacaactgaa agatccgaag atcgccaaac tgttcgatac caacggcgac ggtaaagcgg
     9001 atttaaccgg ttgtaaccct ggctggggct gcgaaggtgc gatcaaccac cagcttgccg
     9061 cgtatgaact gaccaacacc gtgacgcata atcaggggaa ctacgcagcg atgatggccg
     9121 acaccatcag tcgctacaaa gagggcaaac cggtgtttta ttacacctgg acgccgtact
     9181 gggtgagtaa tgagctgaag ccagggaaag atgtggtctg gttgcaggtg ccgttctccg
     9241 cactgccggg cgataaaaat gccgatacca aactgccgaa tggcgcgaat tacggcttcc
     9301 cggtcagcac catgcatatc gttgccaaca aagcctggac cgagaaaaat ccggcggcag
     9361 ccaaactgtt tgccattatg cagttgccag tggccgatat taacgcccag aacgccatta
     9421 tgcatgacgg caaagcctca gaaggcgata ttcagggcca cgttgatggc tggatcaaag
     9481 cccaccagca gcagttcgat ggctgggtga atgaggcgct ggcagcgcag aagtaatttt
     9541 tattcgggcg gataaggcgt tttcgcctca tccgccgctc tgtacacaat gcctgatgcg
     9601 acgctgacgc gtcctatcat gcctacaaac cacatcgcac atttcaacaa gctatctttc
     9661 atcccatatt catcaacatc cgctattatt gatttccagc ttaatcatca cctgatgaac
     9721 aaaaataatg actaaaccta atcatgagct tagcccggcg ctgatcgtgc tgatgtctat
     9781 cgccaccggt ctggcggtag ccagtaacta ttacgcccag ccattgctcg acaccatcgc
     9841 gcgtaacttt tccctttccg ccagttcggc aggctttatt gttaccgccg cgcagttggg
     9901 ctatgccgca ggtctactgt ttcttgttcc cctcggtgat atgtttgaac gccgccgcct
     9961 gattgtctcg atgaccttac tggcagcggg cggtatgtta atcactgcca gcagtcagtc
    10021 actggcgatg atgatcctcg gtacggcatt aaccggttta ttctcagtcg tggcacaaat
    10081 tctggttccg ctggcagcga cgctggcttc accggataaa cgcggcaaag tggttggcac
    10141 cattatgagc ggtctgctgt tggggatatt gctggcacga accgttgccg ggttactggc
    10201 aaacctcggc ggctggcgca ccgtcttttg ggttgcttcg gtgttaatgg cactgatggc
    10261 gctggcatta tggcgtggtc tgccacaaat gaaatcagaa acccacctca actacccaca
    10321 gttgttgggt tccgttttca gcatgttcat cagcgataag atcctgcgca cccgcgcgtt
    10381 gctgggctgc atgacctttg ccaacttcag cattctctgg acctcaatgg cctttttgct
    10441 tgccgctcca ccttttaact acagcgatgg tgacattggt ctgtttgggc ttgcgggagc
    10501 tgccggagcg ttgggcgctc gtccggcggg cggttttgcc gacaagggca aatcgcacca
    10561 caactttcgg tctgcttctg ctgttacttt catggctggc aatctggttt ggtcacactt
    10621 ccgtactggc gttgattatc ggcattctgg tgctggacct caccgtacag ggcgtgcata
    10681 tcactaacca gacggtaatt tatcgaatac atcctgatgc gcgtaatcgc ctgaccgcag
    10741 gttacatgac cagctacttt attggcggtg ccgccggttc gctaatttca gcctcagcct
    10801 ggcaacatgg cggttgggct ggcgtttgtc tggctggcgc gacgattgcc ctggttaact
    10861 tactggtctg gtggcgaggt tttcatcgtc aggaagccgc aaattaagca atcagcaacc
    10921 gttttcgtgg ctttacactt ataagggtgt taagaagccc atcagtctga taaggttaag
    10981 atattcattc agtctattta taatattaac aatcgttaag cgtacactct atggaaagcc
    11041 ctactccaca gcctgctcct ggttcggcga ccttcatgga aggatgcaaa gacagtttac
    11101 cgattgttat tagttatatt ccggtggcct ttgcgttcgg tctgaatgcg acccgtctgg
    11161 gattctctcc tctcgaaagc gtttttttct cctgcatcat ttatgcaggc gcgagccagt
    11221 tcgtcattac cgcgatgctg gcagccggga gtagtttgtg ggttgctgca ctgagcgtca
    11281 tggcaatgga tgttcgccat gttttgtatg gcccgtcact gcgtagccgt attattcagc
    11341 gtctgcaaaa atcgaaaacc gccctatggg cgtttggcct gacggatgag gtttttgccg
    11401 ccgccaccgc aaaactggta cgcaataatc gccgctggag cgagaactgg atgatcggca
    11461 ttgccttcag ttcatggtca tcgtgggtat ttggtacggt aataggggca ttctccggca
    11521 gcggcttgct gcaaggttat cccgccgttg aagctgcatt aggttttatg cttccggcac
    11581 tctttatgag tttcctgctc gcctctttcc agcgcaaaca atctctttgc gttaccgcag
    11641 ctttagttgg tgcccttgca ggcgtaacgc tattttctat tcccgtcgcc attctggcag
    11701 gcattgtctg tggctgcctc actgcgttaa tccaggcatt ctggcaagga gcgcccgatg
    11761 agctatgagg ttctgctgct tgggttactg gttggcgcgg cgaattattg cttccgctat
    11821 ttgccgctgc gcctgcgtgt gggtaatgcc cgcccaacca aacgtggcgc ggtaggtatt
    11881 ttgctcgaca ccattggcat cgcctcgata tgcgctctgc tggttgtctc taccgcacca
    11941 gaagtgatgc acgatacacg ccgtttcgtg cccacgctgg tcggcttcgc ggtactgggt
    12001 gccagtttct ataaaacacg cagcattatc atcccaacac tgcttagtgc gctggcctat
    12061 gggctcgcct ggaaagtgat ggcgattata taacccacaa gaatcatttt tctaaaacaa
    12121 tacatttact ttatttgtca ctgtcgttac tatatcggct gaaattaatg aggtcatacc
    12181 caaatggata gttcgtttac gcccattgaa caaatgctaa aatttcgcgc cagccgccac
    12241 gaagattttc cttatcagga gatccttctg actcgtcttt gcatgcacat gcaaagcaag
    12301 ctgctggaga accgcaataa aatgctgaag gctcagggga ttaacgagac gttgtttatg
    12361 gcgttgatta cgctggagtc tcaggaaaac cacagtattc agccttctga attaagttgt
    12421 gctcttggat catcccgtac caacgcgacg cgtattgccg atgaactgga aaaacgcggt
    12481 tggatcgaac gtcgtaaaag cgataacgat cgccgctgcc tgcatctgca attaacggaa
    12541 aaaggtcacg agtttttgcg cgaggtttta ccaccgcagc ataactgcct gcatcaactc
    12601 tggtccgcgc tcagcacaac agaaaaagat cagctcgagc aaatcacccg caaattgctc
    12661 tcccgtctcg accagatgga acaagacggt gtggttctcg aagcgatgag ctaacgcgtc
    12721 atctcgctca aaaatccaga tttataaaag aaaaaatgac cggccagcat cgcaacatgc
    12781 tggccttttt ggcaagcagg tcggctcagc cgatgagtta agaagatcgt ggagaacaat
    12841 atgagcgcaa atgcggagac tcaaaccccg cagcaaccgg taaagaagag cggcaaacgt
    12901 aagcgtctgc tcctccttct caccttgctc tttataatta ttgccgtagc gatagggatt
    12961 tattggtttt tggtactgcg tcatttcgaa gaaaccgatg acgcatacgt ggcagggaat
    13021 cagattcaaa ttatgtctca ggtgtctggc agcgtgacga aagtctgggc cgataacacc
    13081 gattttgtaa aagaaggcga tgttctggtc actctcgacc cgacagatgc tcgccaggcg
    13141 tttgaaaaag ccaaaactgc actggcttcc agcgttcgcc aaacccacca gctgatgatt
    13201 aacagcaaac agttgcaggc gaatattgag gtgcaaaaaa tcgccctcgc gaaagcacaa
    13261 agcgactaca accgccgtgt gccgctgggc aatgccaacc tgattggtcg cgaagagctg
    13321 caacacgccc gcgacgccgt caccagtgcc caggcgcaac tggacgtcgc tattcaacaa
    13381 tacaatgcca atcaggcgat gattctgggg actaaactgg aagatcagcc tgccgtgcaa
    13441 caggctgcca ccgaagtacg taacgcctag ctggcgctgg agcgtacccg tattgtcagt
    13501 ccgatgaccg gttatgtttc ccgtcgtgcc gtgcaaccgg gtgcgcaaat tagcccaacg
    13561 acgccgctga tggcggtcgt tccagccacc aatatgtggg tggatgccaa ctttaaagag
    13621 acgcagattg ccaatatgcg catcggtcag ccggtcacca tcaccaccga tatttacggc
    13681 gatgatgtga aatacaccgg taaagtggtt ggtctggata tgggcacagg tagcgcgttc
    13741 tcactgcttc cggcgcaaaa tgcgaccggt aactggatca aagtcgttca gcgtctacct
    13801 gtgcgtatcg aactggacca gaaacagctg gagcaatacc cgctgcgcat tggtttgtcc
    13861 acgctggtga gcgtcaatac cactaaccgt gacggtcagg tactggcaaa taaagtacgt
    13921 tcaacaccgg tcgcggtaag taccgcgcgt gaaatcagcc tggcacctgt caataaactg
    13981 atcgacgata tcgtaaaagc taacgctggc taatccagag gtgcgtgtga tgcaacagca
    14041 aaaaccgctg gaaggcgcgc aactggtcat tatgacgatt gcgctgtcac tggcgacatt
    14101 catgcaggtg ctgaactcca ccattgctaa cgtggcgatc cccactatcg ccgggaatct
    14161 gggctcatcg ctcagccaag gaacgtgggt aatcacttct ttcggggtgg cgaatgccat
    14221 ctcgatcccg cttaccggct ggctggcaaa gcgcgtcggg gaagtgaaac tgttcctttg
    14281 gtccaccatc gcctttgcta ttgcgtcttg ggcgtgtggt gtctccagca gcctgaatat
    14341 gctgatcttc ttccgcgtga ttcaggggat tgtcgccggg ccgttgatcc cgctttcgca
    14401 aagtctattg ctgaataact acccgccagc caaacgctcg atcgcgctgg cgttgtggtc
    14461 gatgacggtg attgtcgcgc caatttgcgg cccgatcctc ggcggttata tcagcgataa
    14521 ttaccactgg ggctggatat tcttcatcaa cgtgccgatt ggcgtggcgg tggtgttgat
    14581 gacactgcaa accctgcgcg gacgtgaaac ccgcaccgaa cggcggcgga ttgatgccgt
    14641 ggggctggca ctgctggtta ttggtatcgg cagcctgcag attatgctcg accgcggtaa
    14701 agagctggac tgtttttcat cacaggaaat tatcattctt accgtggtgg cggtggtggc
    14761 tatctgcttc ctgattgtct gggagctgac cgacgataac ccgatagtcg atctgtcgtt
    14821 gtttaagtcg cgcaacttca ccatcggctg cttgtgtatc agcctcgcgt atatgctcta
    14881 cttcggcgct attgttctgc tgccgcagtt gttgcaggag gtctacggtt acacggcgac
    14941 ctgggcaggt ttggcctctg cgccggtagg gattattccg gtgattctgt cgccgattat
    15001 cggccgcttc gcgcataaac tggatatgcg gcggctggta accttcagct ttattatgta
    15061 tgccgtctgc ttctactggc gtgcctatac ctttgaacca ggtatggatt tttgcgcgtc
    15121 ggcctggccg cagtttatcc aggggtttgc ggtggtctgc ttctttatgc cgctgaccac
    15181 cattacgctg tctggtttgc caccggaacg actggcggcg gcatcgagcc tctctaactt
    15241 tacgcgaacg ctggcggggt ctatcggcac gtcgataacc acgaccatgt ggaccaaccg
    15301 cgagtcgatg caccatgcgc agttgactga gtcggtaaac ccgttcaacc cgaatgccca
    15361 ggcgatgtac agtcaactgg aagggcttgg gatgacgcaa cagcaggcat caggctggat
    15421 tgcccagcag atcaccaatc aggggctgat tatttccgcc aatgagatct tctggatgtc
    15481 agccgggata ttcctcgtac tgctggggct ggtgtggttt gctaaaccgc catttggcgc
    15541 aggtggcggc ggaggcggtg cgcactaagt acaactaagc cagttcattt gaactggctt
    15601 ttttcaatta attgtgaaga tagtttactg actagatgtg cagttcctgc aacttctctt
    15661 tcggcagtgc cagttcttcg ttgctgttga tgcgtacctc acgttccaga atgctacgcg
    15721 caatatcctg cgcttcctgc aacgagtgca tctggtaagt gccacactgg tagacgttca
    15781 gttccgggat ctgattctga tcctgcactt tcagcacgtc ttccattgcc gctttccagg
    15841 catcagcaac acgctgctca tctggcgtac caatcagact catataaaaa ccggtgcggc
    15901 agcccattgg cgagatatcg ataatctcta caccattacc gttaagatgg ttacgcataa
    15961 aaccagcaaa caggtgctcc agggtatgga tccctctttc tggcatcact tctttgttcg
    16021 gcacgcagaa gcgcagatcg aacacggtga ttgcgtcgcc atgcggggtg ttcattgttt
    16081 tcgccacccg aactgcaggc gcttccatcc gggtatgatc gactgtgaag ctatctaaca
    16141 acggcattta gccacctccg gtaatttttt taaaaatttt ctgaactctt tcttcccagg
    16201 cgagtctgag tatatgaaag acgcgcattt gttatcatca tccctgaatt cagagatgaa
    16261 attttggcca ctcccgagtg gcctttttct tttctgtcag gcgtgttttt ccagccacac
    16321 cgcaaacggt tcggtatcag cggtttccat ttcctgctga cggcgttcag atgcctcgcg
    16381 ctcggctaca aaatcctctt cgcgcagaat ttccagcggc tcttcacgca gcagattacg
    16441 gtaggcttct gcaaacgctt tgcctgttcc gccaatacca gtatcaatca tagaccttaa
    16501 gatacgggca gagaaagtca gatcgggatt atcgaagcag gcaaccagtt catcacacac
    16561 tttctgatac gcttcgccgc cgttaatgct atccagcgtt tgcgcgacgc gtttcagatc
    16621 gcggaacaga tctttaccca cctgcagtaa cgggaactgc gcggtttcgc agccgatacc
    16681 cagcgtcagg cccggtttgc gaccttcaag gatcacccgg ttccagttaa cgcgcgtaca
    16741 ggcaagttcg ctactgctca tttccggtgc atcagccagc gcacaccaga ccataaacag
    16801 gtcgaggaat cgcacctgct gttcatctac accaatcggc gagaacgggt tgatgtccag
    16861 cgaacgcact tcaatatatt caatgccgcc acgtaacagc gcatcagaag gtgactcgcc
    16921 gctgcgggta acgcgttttg gacgaatcgg cgcatacagt tcgttttcaa tctgcagcac
    16981 gttgctattg atttgcagcc tcttaccgtc tttctcaata ccaatcttcg cgtactcttc
    17041 cgatggcgtt ttgattgcct gtttaaggcc cgctacgtac tcgtaaagat cgttgaaggt
    17101 aataccaaga ttgctttgcg atttattggt atagccgaga tcgctcaaac gaagagaggt
    17161 cgcatacggc aggtaataca taccgcactc ggttttctca aacggcagcg acgttggttt
    17221 tccttgcagg aaagaagaac aaatcgccgg agatgcacca aacagataag gaataaccca
    17281 accgaaacga tagtaattgc ggataacgcg gaaatagccc gcagaaattt tctctttggc
    17341 atcagcgccc gagatatcac cgcacttcgc ttgccagaat gccattggca aagagaaatt
    17401 gtagtgcacg ccggaaatgg tttgcatcag cgcgccgtag cgatttttca gcccttcacg
    17461 atacagcgtt ttaaagcgtc cggtgttaga agtgccgtac tgtgccagtt cgatgtcctg
    17521 accttctgcg atgtagcatg gcatacttaa cggccacatc cgctcatcgc ccatattgcg
    17581 cgccgtataa cgatgcagat cgcgcataaa ggtcagcata tgttcaatat caccatccac
    17641 tggtgtaatg aattccagca atgcttccgc aaaatcggta gtaatccatt tgtgcgtcag
    17701 tgcggaacct aatgcttcag gatgacctgt tgttgccagt gtgccatcag cattaacacg
    17761 caaagtttcg cgctccagcc cacgctgtat cccctttaac gcctgaggat gtttttccag
    17821 ccaggccagc gcctgtgata cgtccgggat caaattgacc tcccgcctgt caaaatcgtt
    17881 ttaattagca taactgtaat ggtgaccata tgtgcaggcc tacaattagt gccaccacat
    17941 catgccctga acggtcgctg ctgcaactgc aacatagcgt aacgctttac caaggcacaa
    18001 aaaaaagatt actggccccc aggagatgcg catccatccc gctaacagac acagtaaatc
    18061 gccaaccacg ggcatccagc ttaataatag cgtgactgca ccatagcgtt tcagccagcc
    18121 ggtagctttc tcttgccagc gcgatgtttt acgcaatgga aagaaacgcc caaggataac
    18181 gttagttaac cctccaaggc tattacccat tgttgctgtt aaaactaaaa cccagggatg
    18241 actgatcccg gaaagcaaca ttgccaccag cacgacttcg gagttgccgg gtaatagtgt
    18301 agcgctgaga aaactactgg caaacaacga gaaaagcgat aacgcttcac tcacagcaag
    18361 cgaacatcca cagcgtccat gcctgctgca cgggccgcct gaataccgaa atcggcatct
    18421 tcaaagacca cacactgcgt cggttgcacg cccatacgct gcgcgcacaa caaaaatgtg
    18481 tctggcgcgg gtttatggtg tttgacgtga tcagcagcga cgacggcgtc aaaataacgg
    18541 cgtaatccca ggtgcgccag caatgcctca gcgatggcgc tttcactccc cgttcctaca
    18601 gccattgggc gacgaccatg ccaacttttc accacttcaa caagaggaag cggctcgaca
    18661 ctatccagca gcatacttct tactgcttct gttttttcac gcgctaacgc atgcgggtcg
    18721 agatcggcct gattcagctc aataattgcc tgagcaatac gccaggtggg cgatccatta
    18781 agcgcaatca tcgcctgaat atcgtactga agaccgtagt gccctaatac ttcgcgccac
    18841 gctttacggt gcgtaggcgc cgtatccagg attgtgccat ccatatcaaa aattaaacct
    18901 gcataacgct cgtacatggt cttctcgcaa gtcgaaaaat atgacgctac tttagcgtaa
    18961 ttgcttgatt ttgtcgctga tggcgaagga gaagaaaatt caggaaatgg ataaagtagt
    19021 tatttagaaa tgagatattt ttgaaggaaa ttttttggag aatatggtgc atccgggagg
    19081 attcgaacct ccgaccgctc ggttcgtagc cgagtactct atccagctga gctacggata
    19141 catcgggaaa cttactttac tgcagatttt ttgataccgc tactaaagcc gtatcaagta
    19201 agagatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc
    19261 tatccagctg agctacggat gcatcgggaa acttacttta ctgcagattt tttgataccg
    19321 ctactaaagc catatcaagt aagagatggt gcatccggga ggattcgaac ctccgaccgc
    19381 tcggttcgta gccgagtact ctatccagct gagctacgga tgcatcggga aacttacttt
    19441 actgcagatt ttttgatacc gctactaaag ccgtatcaag taagagatgg tgcatccggg
    19501 aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc tgagctacgg
    19561 atgcaaatgg cggtgaggcg gggattcgaa ccccggatgc agcttttgac cgcatactcc
    19621 cttagcaggg gagcgccttc agcctctcgg ccacctcacc acacgcctct tacgagtgct
    19681 tcgaagaact tgtttatcgc tcatcatcgc tgcgtggcgc acatattact ttctgagact
    19741 tataagtcaa acaatttttc ccacactttt atcgtttgca cacttcacgc tcaattagtc
    19801 tgtaaaagcg tcaaaaagag tgttttatct acagaaaaac ggaggtctgg cagatagcag
    19861 taatgcaaaa aaatggagac ttaagttgaa tgaacgggag taaagcgaaa agactataga
    19921 gtgaaggaga aattttgagg gtgcgtctca ccgataaaga tgagacgcgg aaagattagt
    19981 aactggactg ctgggatttt tcagcctgga tacgctggta gatctcttca cggtgaacag
    20041 aaacttcctt cggggcattt acgccaatac gtacctggtt gccctttacc cctaaaactg
    20101 tcacggtgac ctcatcccca atcatgaggg tctcaccaac tcgacgagtc agaatcagca
    20161 ttctttgctc cttgaaagat taaaagagtc gggtctctct gtatcccggc attatccatc
    20221 atataacgcc aaaaagtaag caatgacaaa cacattacat gtaagcagtc atggcattac
    20281 attctgttaa acctaagttt agccgatata cacaacttca acctgacttt atcgttgtcg
    20341 atagcgttaa tgcgaatgcc gtgaagcgag tccacggcat tgcctgacgc ttatattatt
    20401 gcaatttcgc gctgacccag cctttcacac tggctaacgc tgcaggtaag gccgcagcat
    20461 ccgtaccacc ggcttgcgcc atgtcaggac gtccaccacc cttgccgccc acctgctgag
    20521 cgaccatacc aatcagttcc cctgctttca cacgatctgt gacgtcctta gatacgcctg
    20581 caatcagaga aaccttacct tcggctaccg ttgccagcac gataattgtc gaccccagct
    20641 gattttttaa atcgtcaacc atggtacgca acattttcgg ctcaacaccg ctaagctcgc
    20701 taaccaacag cttaacacca ttaacatcaa ttgccttact ggaaagattt gcgctctcct
    20761 gtgcggcagc ttgttcttta agctgttgta gttctttttc taactgacgc gtacgttcca
    20821 gtactgagcg cactttatcc gccagattat tgctatcgcc tttcagcaga tgcgcgactt
    20881 cgcttaagcg atcgctgtct gcatgaacgg tggtgatagc gccttctccg gttaccgctt
    20941 cgatacgacg aacgcctgca gcagtacccg attcagagat gatgcggaac agaccaatat
    21001 caccagtgcg gctggcgtga gtaccgccac acagctcggt ggagaaatcg cccatgctca
    21061 gcacgcgtac acgctcatca tacttctcgc cgaacagcgc catcgcacct ttcgctttcg
    21121 ccgcttcgag attcatgatg ttggtttcga tcggcaaatt gcgacgaatc tgtgcgttca
    21181 ccaggtcttc gaccgcacga atctcttccg gtttcatcgc ttcgttgtgt gagaagtcga
    21241 agcgcagcac cttgtcgtta accagtgaac ctttctgcga tacatgagtc cccagaacct
    21301 ggcgcagcgc agcgtgcatc aggtgcgttg cggagtgatt cagacgaata cgggcgcgac
    21361 gagcctcatc aatatcagcc tgcacagcgt cgcccacttt cagagaaccc gcagcaagtt
    21421 taccgatgtg accaatcgcc tggccgtatt tctgcgtatc ttccaccgcg aaggagaagt
    21481 tagcgccttt cagttcgcct ttatcaccaa cctgaccgcc ggattccgca tagaatggcg
    21541 tttgatccag cacgaccaca gcttcctggc cggcattgat ggcatcaacc gctttaccat
    21601 caacaaacag cgcggtcact ttgccgttca gttccagatg gtcatagcct ttaaattcag
    21661 atgcactatc aacacggatc attgcgttgt aatcggcacc aaagccgctg gcttcgcgcg
    21721 cgcgacgacg ctgctcttcc attgctgctt caaaaccagc ttcgtcaact ttgatgttgc
    21781 gctcacgaca aacatcagcc gtcaggtcaa ccgggaagcc ataggtgtcg tacagacgga
    21841 aagccgtttc accatccagc gtatcaccag aaagttttgc cagctcttca tccagcaacg
    21901 ccagaccgcg ctccagagta cgggcaaact gctcttcttc agtcttcagc acctgctcaa
    21961 cctgcgcctg ctggcgtttc aggtcttcac ccgcagagcc cataacgtcg atcagcggac
    22021 caaccagttt gtagaagaag gtttctttcg cgccgagcat attgccgtga cgcactgcgc
    22081 gacgaatgat acgacgcagt acataaccac ggttttcatt ggacggcatt acgccatccg
    22141 cgatcaggaa cgcacaagaa cgaatgtggt cagcgattac gcgcagcgat ttattgctca
    22201 gatcggtcgc gccagtgact ttcgctaccg cctggatcaa cgtgcggaac aggtcgatgt
    22261 catagttaga gttaacgtgt tgcagcaccg cagcaatacg ctccagaccc ataccggtat
    22321 ctacagacgg cttcggcagc ggttccatcg tgccatctgc ctggcggttg aactgcatga
    22381 agacgatgtt ccagatctca atgtagcggt cgccgtcttc ttccggactt cccggagggc
    22441 caccccaaat gtggtcgccg tgatcgtaga agatttcggt gcacgggccg cacggaccag
    22501 tgtcacccat ctgccagaag ttgtcagatg cgtatggcgc acctttgtta tcgccgatgc
    22561 gaataatacg ttcgcgcggg atcccgactt ctttttccca gatttcgtag gcttcgtcgt
    22621 cgctttcata gacggtaacc cacagacgct ctttcggcag ggcaaaccat ttttcgctgg
    22681 tcagcagttc ccatgcaaac tgaatggcat cgtgtttgaa atagtcgccg aagctgaagt
    22741 tgcccagcat ttcgaagaag gtatggtgac gcgcggtgta accgacgttt tccaggtcgt
    22801 tgtgtttacc acccgcacgc acgcagcgtt gggaagtggt agcgcgggaa taattacgct
    22861 tgtcgagccc aaggaacaca tccttgaact ggttcatccc ggcgttggta aacaacaaag
    22921 ttgggtcgtt atggggtacc agggagctgc tggcaactac ctgatgtccc ttactatgga
    22981 aaaagtcgag aaacgcctga cggatctcag cggtgctctt gctcataatt atcctgaaat
    23041 caagctaacg aaatatcgcc accagctcca gcgtgtctta accgccgggc tggtaactga
    23101 aaagtgggaa taagataagt tttcttgact gggaagtaaa ataccgtatg cgttcagtcg
    23161 gcaaaatttc gccaaatatc ctggatatct tccatcagat agccacgata gagcagaaaa
    23221 cgctggatct taactttttc tgaaaagaca gttggcaaag gttcaccata ttttcgcgtc
    23281 gcctgatcgc gcgccagtgc gcaccagtcg atgtcacatt cacgcatcgc tttttctgtc
    23341 gcttcgcggg aaataccttt ctgattcagt tcctgacgaa tacgtgcagg tccataccct
    23401 ttgcggctac ggctggcgat aaagcgcgca acaaatcggc tgtcatcgag atagccatgt
    23461 tcatggcacc aggcaataac gcgctcgtaa tcttctgccg tagcatcaat ctcttctggg
    23521 ccatttttgc ccataatcgg tgccgcgagt ttacgtcgca gttcttgctc actgtgatcg
    23581 cgcaccgcca gaatgcgtac cgcacgatcc aacaggcgag catatgccgg gcgacgggat
    23641 gttgattctg tcatggcata tccttacaac ttaaaaaagc aaaagggccg cagatgcgcc
    23701 ccaggttttg ctgataaaca gatttaatag tcaatgagta tgcagaatgt tcccgcaggc
    23761 ccaattcgtt agaggatcga ggtgagagta atttttcacg ccagtgctgg cctgttcccc
    23821 tcaccctaac cctctcccca aaggggcgag gggaccgtcc gtgcacacat tatctattat
    23881 catcaataac gaacgattaa aaatcttcgt tagtttctgc tactccttcg ctgtcatcta
    23941 cagagaaatc cggcgttgag ttcgggttgc tcagcagcaa ctcacgtact ttcttctcga
    24001 tctctttcgc ggtttccggg ttatctttca gccaggcagt tgcattcgct ttaccctgac
    24061 cgatcttctc acctttgtag ctgtaccacg cgcctgcttt ctcgatcagc ttctctttta
    24121 cgcccaggtc aaccagttcg ccgtagaagt tgataccttc gccgtagagg atctgaaatt
    24181 cagcctgttt aaacggtgca gcgattttgt tcttcaccac tttcacgcgg gtttcgctac
    24241 ccaccacgtt ttcgccctct ttcaccgcgc cgatacgacg gatgtcgaga cgaacagagg
    24301 cgtagaattt cagcgcgtta ccaccggtag tggtttccgg gttaccgaac atcacaccaa
    24361 ttttcatacg gatctggttg atgaagatca gcagcgtgtt ggactgcttc aggttacccg
    24421 ccagcttacg catcgcctgg ctcatcatac gtgccgcaag gcccatgtga gagtcgccga
    24481 tttcgccttc gatttccgct ttcggcgtca gtgccgccac ggagtcaacg acgataacgt
    24541 ctactgcgcc agaacgcgcc agggcgtcac agatttccag tgcctgctcg ccggtatccg
    24601 gctgggagca cagcaggtta tcgatatcga cgcccagttt acgtgcgtag attgggtcca
    24661 gcgcgtgttc agcatcgata aacgcacagg ttttaccttc acgctgcgct gcggcgatca
    24721 cctgcagcgt cagcgtggtt ttaccggaag attccggtcc gtagatttcg acgatacggc
    24781 ccatcggcag accacctgcc ccaagcgcga tatccagtga aagcgaaccg gtagagatgg
    24841 tttccacatc catggaacgg tcttcaccca ggcgcatgat ggagccttta ccaaattgtt
    24901 tctcaatctg gcccagtgct gccgccaacg ctttctgttt gttttcgtcg atagccattt
    24961 ttactcctgt catgccgggt aataccggat agtcaatatg ttctgttgaa acaattatac
    25021 tgtatgctca tacagtatca agtgttttgt agaaattgtt gccacaaggt ctgcaatgca
    25081 tacgcagtag cctgacgacg caccgcatca cggtcgccgc tgaagcattc ccgccgggta
    25141 atgccttcac cgcgggcagt ggcaaaagca aaccagacgg tgccgacagg cttctcttca
    25201 ctgccgccat ccggcccggc gataccacta atagacacgg cataatcagc acgagccgct
    25261 ttcagtgcac ctatcgccat ttccaccacg acgggttcac tcaccgcgcc atgctgcgcc
    25321 agcgtctctt cgcgtacgcc gatcatctgc gctttggctt cgttactgta ggtgacaaat
    25381 ccgcgttcaa accaggcgga gctaccggca atatcggtaa tcactttcgc tacccaacca
    25441 ccggtacaag actcggcagt tgttacggtt gcgccacggg ctttcagcgc ctgcccaacc
    25501 tgttcgctta actgcatcag ttcactgtca gtcatcacaa cctctgtcag tcaaaaattt
    25561 atgccggaca agatagcact ttcgtcagga agatggtgtt ggcgttgcaa ttttacgagg
    25621 aggattcaga aaaaagctga ttagccagag ggaagctcac gcccccctct tgtaaatagc
    25681 tactgtactc gcgccagcgc cacggcttgt cctaactgcc agaccgccat tgcgtaatgg
    25741 gtgctgtggt tgtaacgggt gatggtgtag aagttcggca gaccgtacca gtactggtag
    25801 ccggtgccaa catccagacg cagcaggctg gcttgttgat ggttgcccag cggctgctgt
    25861 ggcgttaaac ctgcggcggc aagctgcgaa atgctgtact tagttttgaa accatttggc
    25921 aagcctggag cctgaccgtt tgccattatc gcgacctgat cgcctttcac ccagccgtgc
    25981 gctttgaaat agttcgccac gctaccgatc gcatcaaccg gatcccacag gttgatatgc
    26041 ccgtcgccgc tgaaatctac cgcatattgt ttgtaagacg acggcataaa ctgtccgtag
    26101 cccatcgccc cggcaaagga acctttcaga ttgagcggat cgtcctgctc gtcgcgcgcc
    26161 atcagcagga aggtttccag ctcgccagag aaatactccg cgcggcgtgg gtagttaaat
    26221 gacagcgttg ccagcgcatc gaggatgcga gttttcccca tcacgcgccc ccagcgggtt
    26281 tcaacgccga taatcccgac gataatttcc ggcggtacgc catacacctg ccacgcgcga
    26341 ttcaacgcat cttcatactg attccagaaa accacaccgt tctgcacgtt gtccggcgta
    26401 ataaattttt tgcgataacg cagccatgcg ccgttcggac ctgatggcgg tttcaccgat
    26461 gtggttggtg cctggttatc catcagccgc agtaccgaat ccagacgctt cgcctgggag
    26521 agaatttcct gcaactgctg acgatcgaaa ccgtgtttgt tcaccatttt gtcgatgaac
    26581 tgctgggcat tcgggttatt agcgaaatcg ccgcccatct gcatcacatt gtgctgcggc
    26641 tcaagcagga agccgctaga cggcgttccg gtggtcgttt cagtctcagt aggttttggc
    26701 ttgctgctac aggcggcaag caacacaaaa aggggaagca atgttacata acgacgcttg
    26761 aacatgaggg gtccatttaa cagattcaac caggggcaag tatggtaaag catcacaccc
    26821 cgcacaagga agcggtagtc actgcccgat acggacttta cataactcaa ctcattcccc
    26881 tcgctatact tttattcaaa ctttcaaatt aaaatattta tctttcattt tacgatcaaa
    26941 ataacacttt taaacctttc aacctgatta gattaggttg ccgtttggta ataaaacaat
    27001 aaatcctgaa ggagagaaca atgatagaaa ccattactca tggtgcagag tggtttatcg
    27061 ggctgttcca aaagggcgga gaggtgttta ccgggatggt gaccggcatt cttccgctgt
    27121 tgattagcct gcaggttatc atgaacgcac tgattaattt tatcggtcag catcgtattg
    27181 aacgttttgc tcaacgttgc gccggtaacc ctgtttcccg ttacctactg ttaccgtgca
    27241 ttggcacgtt tgtcttttgc aatccgatga ccttaagcct gggtcgcttt atgccggaaa
    27301 agtacaaacc cagctactac gcggcggcct cttatagctg ccactcaatg aatggcctct
    27361 tcccccatat caaccctgcg aactgtttgt ttatcttggc attgccagcg gtctgacaac
    27421 gctgaacctg ccacttggcc cactggcggt gagttatctg ctggttggtc tggtcaccaa
    27481 tttcttccgc ggctgggtga ccgatctgac caccgccatt tttgagaaaa agatgggcat
    27541 tcaacttgaa caaaaagttc acctggcagg agcaacatca tgacgcgtat tcggatcgaa
    27601 aaaggaacgg gtggctgggg cggcccgctt gagctggaag ccacgccggg caaaaaaatc
    27661 gtctatatca ccgccggtac ccggcctgcg attgttgaca aactggcaca acttactggc
    27721 tggcaggcta ttgacggatt taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg
    27781 gtaatcgact gtggcggcac attacgctgc ggcatctatc cgaaacgacg tattcccacc
    27841 attaatatcc actcgacggg caagtccggt ccgctggcgc agtacattgt ggaagatatt
    27901 tatgtctctg gcgtaaaaga agaaaacatc actgtagtgg gtgatgcgac accacaaccc
    27961 tcttccgtgg gccgtgacta tgacaccagt aagaaaatca ccgaacaaag cgatggttta
    28021 ctggcgaagg tgggaatggg catggggtcc gccgttgccg tgttgtttca gtctggtcgt
    28081 gacaccatcg acactgtatt aaaaaccatt ctgccgttta tggcattcgt ctcggcgctc
    28141 attggcatca ttatggcttc tggccttggt gactggattg cccacggtct tgctccgcta
    28201 gcgagccatc cactgggtct ggtcatgctg gcgctcatct gctccttccc actgctttca
    28261 cctttcctcg gcccaggcgc agttatcgca caggttatcg gcgtattgat tggcgtgcag
    28321 attggtctcg gcaatattcc gccgcatctg gctttaccgg cactgtttgc catcaacgcg
    28381 caggcggctt gcgacttcat cccggtcggt ttgtcgctgg cggaagcccg tcaggacacg
    28441 gttcgcgtcg gtgtcccttc tgtactggtg agccgctttt taaccggcgc gccgactgta
    28501 ctgatcgcct ggtttgtctc cggttttatc tatcaataga ggctgaaaca tgaccgttat
    28561 ttatcagacc accatcaccc gtatcggcgc gagtgccact gacgccctca gcgaccagat
    28621 gctcatcacc tttcgtgaag gcgcgccagc ggacctcgaa gagtattgct tcattcattg
    28681 ccacggcgag ttgaaaggtg cactccatcc cggtttgcaa ttttcactcg ggcagcatcg
    28741 ctatccggtg accgctgttg gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt
    28801 caccctgcgc ttcgatggtt taaacgaagc ggaatttccg ggcactgtcc atgtggcagg
    28861 ccctgtaccc gacgatatcg cgccgggatc ggttttgaag tttgaatctg ttaaggagta
    28921 aaaaatgaat caggttgccg ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg
    28981 ccacggtctg gctgccgagg ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc
    29041 cgcaaatgtg gcacaagaaa ttaacgccga atatggtgaa ggtatggcgt acggttttgg
    29101 tgctgacgcc actagcgagc aaagcgttct ggcgctctct cgtggggtag atgaaatctt
    29161 tggtcgcgtg gatttgctgg tctatagcgc cggaatagca aaagcagcct ttatcagcga
    29221 cttccagctc ggcgattttg accgttcgct acaagtgaat ctggtgggtt atttcctgtg
    29281 tgcccgtgaa ttttcgcgtt tgatgatccg cgacgggatt cagggtcgca ttattcagat
    29341 caactcgaaa tccggcaaag tgggcagcaa acacaactct ggctacagcg cagcgaaatt
    29401 tggtggcgtc gggctgactc aatcactggc gctggatctg gcggagtacg gcattacggt
    29461 gcattcactg atgctcggta acctgctgaa atcgcggatg ttccagtcac tgttgccaca
    29521 atacgcgacc aagctgggta tcaaaccgga tcaagtcgag cagtattaca tcgacaaagt
    29581 accgctcaaa cgcggctgcg attatcaaga tgtgctgaat atgctgctgt tctacgccag
    29641 tcctaaggcg tcgtactgca ccggacagtc gatcaatgtc accggcggtc aggtgatgtt
    29701 ctgatcaaca gcggagatcc gttaaggatc tccgtgagac tatagaatgc ctgatgcgct
    29761 acgctcatca ggcatacagg acttccgcca ctacattaag gaaaagttat ggtatccgca
    29821 ctcatcaccg tcgccgttat cgcctggtgt gcgcaactgg ccttaggcgg ctggcaaatt
    29881 tctcgtttta accgtgcctt cgacacacta tgccagcaag ggcgggttgg cgtggaccgt
    29941 tccagcgggc gctttaaacc gcgggtcgtg gtcgccatcg cgctggacga tcagcagtgc
    30001 atcgtcgaca ccttgtttat gaaaggactg actgtcttcg cccgaccgca aaaaattccc
    30061 gcaattaccg gcatgcatgc gggtgattta cagcccgatg tgatctttcc ccatgatcca
    30121 ctatcacaga atgctctatc attggcgctt aaactgaaac gtggataatt tcgttgtgaa
    30181 tgttacttgc ttgcgaagtt atcattttga aacctaaatc aggtaatcac gcccatgaaa
    30241 cctcgtcagc gtcaggccgc cattctggag tatctgcaaa agcagggtaa atgctcggtt
    30301 gaagaattgg cgcaatactt tgacaccaca ggcacaacca ttcgcaaaga tctggtcatt
    30361 ctggaacatg ccggaaccgt cattcgtact tatggcggag tggtgttgaa taaagaggaa
    30421 tccgatccgc ctatcgatca taaaacgctc atcaacaccc acaagaaaga gctgattgca
    30481 gaagctgccg ttagttttat ccatgatggc gattcgatca ttcttgatgc tggcagtacc
    30541 gttttgcaga ttgttcccct gctctcgcgc tttaataaca tcacggtgat gaccaacagc
    30601 ctgcatatcg tcaatgcgct atccgaactg gataacgaac aaactatcct gatgccaggc
    30661 ggaacatttc gcaaaaaatc ggcctcattt cacgggcaac tggcagagaa tgccttcgag
    30721 catttcacct tcgataaatt gtttatgggc accgacggca tcgatctcaa tgcgggcgta
    30781 accacgttca acgaggttta taccgtcagt aaggcaatgt gcaatgccgc gcgcgaagtg
    30841 attttgatgg cggactcatc aaagtttggc cgtaaaagcc ccaacgtagt ttgcagtctt
    30901 gaaagcgtcg ataagctaat taccgacgca ggtatcgatc cggcgtttcg tcaggcgctg
    30961 gaagagaaag ggatcgatgt gatcataacc ggagagagca atgagtgaag cactactgaa
    31021 cacgggacgt cagacgttaa tgctggagtt gcaggaagca agccgtttac cggaacgtct
    31081 gggcgatgat tttgttcgcg ccgccaatat catcctgcac tgcgaaggca aagtggtggt
    31141 ttcgggaatt ggcaaatcgg gccacattgg taagaaaatc gccgcaacac ttgccagtac
    31201 cggcacaccg gctttttttg tccatccggc agaagcactg cacggcgatc tggggatgat
    31261 tgaaagccgc gatgtgatgc tgtttatctc ttactccggt ggcgcgaagg aactggatct
    31321 gattattccg cgtctggaag ataaatctat cgcgctgctg gcgatgaccg gcaaaccgac
    31381 gtcaccgctg ggcctggcgg cgaaagcggt gctggatatc tccgtagaac gcgaagcctg
    31441 cccgatgcac cttgcgccga cctccagcac cgtcaatacc ctgatgatgg gtgacgcgct
    31501 ggcgatggcg gtcatgcagg cgcgcggttt taatgaagaa gattttgccc gctcccaccc
    31561 agctggggca ctgggcgctc gcttgctgaa taaagtgcat catctgatgc gtcgtgatga
    31621 cgctatccca caggtagcgt taaccgccag cgtgatggat gcgatgctgg aactcagccg
    31681 caccggtctg gggctggtgg cggtatgtga cgctcaacaa caggtacaag gcgtctttac
    31741 cgacggcgat ttacgtcgct ggctggtagg cggcggcgca ctcaccacgc cagttaatga
    31801 agcgatgacg acaggcggca ccacattaca ggcacaaagt cgcgccatcg acgccaaaga
    31861 agttctgatg aaacgcaaaa tcactgccgc accagtggta gatgaaaacg gcaaactcac
    31921 cggcgcaatt aacctgcagg atttctatca ggccgggatt atttaatcct tcaatcccag
    31981 acgtttcgcc agccgatgca ggttggcgac gtcagtttcc agcattcgcg cacaggctgc
    32041 ccagttgtga tgattttgtg ccagtgcctg gcgaatagtt tcacgctgga acgcttctgt
    32101 cgcttcacgc aggttttgct taacaacggg caccgccgcc gcttctggcg gcggcaacgt
    32161 cacctcagga aaagcaaaat gttgcgcctc aagaatcact tcatcgccgt tgcgggtggc
    32221 tctcgccagc actaccgccc gatgaatagc atgttccagt tcgcgcacgt ttcccggaaa
    32281 actgtagtgt tgcagtaaat ttcgcgcccc ggcacttaat accacgcggg agagcccctg
    32341 ccgcaaacga cactgctcgc agaaataccc cgccagcaga atgacatcat cgccccgctc
    32401 acgcagcggc ggcaccgaaa gtggaaacac gctcaggcga tgaaacaaat cggcgcggaa
    32461 tcgccctgcc agcacctctt cgcgtaagtc gcggttagtc gccgccagca cgcgcacatc
    32521 gacccgcaaa ctacggtcat cgccaacgcg ctgaatatcg ccatactgca acaccctcag
    32581 cagcttggcc tgcaatgcca acgacaactc gccgatctca tcgagaaaca gagtaccgtt
    32641 atccgccatt tcaaacttcc cgctgcggtt actgatagcg ccagtaaacg ctcctttcac
    32701 atgcccgaac aactcacttt ccgccacact ttccggcagt gcggcacagt tgagatagac
    32761 cagcggattc accgcccgtg gcgaggcttc atgaatcgct ttcgccacca gctccttacc
    32821 ggttcccgtc tcaccgctga tcagaacgtt gagatcggac gccgccacaa tctcaatctc
    32881 ttttttcagt tgcgtcatgc caggggacaa gccaatcatc tgcgtctgtt tcaccgcttc
    32941 aaacggcgtg gcatcgcctg gcagcatatt ctggctttcc agttgttcaa tcagcaacgc
    33001 attgcttaac gctcccgccg ccagcgcggc aatcagccgt aactcttcgt cgctgaaaac
    33061 atcgaactga tcgggctgca tcccgtcgag cgtcagtgcg ccgatcaggt tttgtccgac
    33121 aaacaatggc agaccaacgc aggcgtgaac cttcagactc tcctgcccag gaatcaaacc
    33181 gtcataggga tcgggcaatt cgctgtctgc gggaaagcgc accacatccc cggcgcgggc
    33241 aatcgcttcc agccgtggat gcccttccag cgcaaagcgt ctaccgagta catcctttgc
    33301 cagaccgtcg atggcaagcg gaataaactg ccgcgaatcg taacgtagca acgcagacgc
    33361 atcgcactcc agcacctgac gtagcgtggt gatcaggcgc tgaaaacgat cctgatgacc
    33421 aatcccacgc tgcaattcga tggcgatatt cgccagcaca tcaacggaaa aactcatctt
    33481 tgcctcactg tcaatttgac tatagatatt gtcatatcga ccatttgatt gatagtcatt
    33541 ttgactactc attaatgagc ataattttat ttatagagta aaaacaatca gataagaaac
    33601 tggcacgcaa tctgcaatta gcaagacatc tttttagaac acgctgaata aattgaggtt
    33661 gctatgtcta ttgtggtgaa aaataacatt cattgggtgg gtcaacgtga ctgggaagtg
    33721 cgtgattttc acggcacgga atataaaacg ctgcgcggca gcagctacaa cagctacctc
    33781 atccgcgaag aaaaaaacgt gctgatcgac accgtcgacc ataaattcag ccgcgaattt
    33841 gtgcagaacc tgcgtaatga aatcgatctg gcggatatcg attacatcgt gattaaccat
    33901 gcagaagagg accacgccgg ggcgctgacc gaactgatgg cgcaaattcc cgatacgccg
    33961 atctactgta ctgccaacgc tatcgactcg ataaatggtc atcaccatca tccggagtgg
    34021 aattttaatg tggtgaaaac tggcgacacg ctggatatcg gcaacggcaa acagctcatt
    34081 tttgtcgaaa caccaatgct gcactggccg gacagcatga tgacttacct gacaggcgac
    34141 gcggtgctgt tcagtaacga tgctttcggt cagcactact gcgacgagca tctgttcaac
    34201 gatgaagtgg atcagacgga gcttttcgag cagtgccagc gttactacgc caatatcctg
    34261 acgccgttca gccgcctggt aacgccgaaa attaccgaga tcctgggctt taacttacca
    34321 gtcgatatga tagcgacctc ccacggcgtg gtctggcgcg ataacccgac gcaaattgtc
    34381 gagctgtacc tgaagtgggc cgctgattat caggaagaca gaatcaccat tttctacgac
    34441 accatgtcga ataacacccg catgatggct gacgctatcg cccagggaat tgcggaaacc
    34501 gacccacgcg tggcggtgaa aattttcaac gtcgcccgaa gcgataaaaa cgaaatcctg
    34561 accaatgtct tccgctcaaa aggcgtgctg gtcggcactt cgacgatgaa taacgtgatg
    34621 atgccgaaaa tcgccgggct ggtggaggag atgaccggat tacgcttccg taacaaacgc
    34681 gccagtgctt tcggatctca cggctggagc ggcggtgcga tggatcgcct ttccacgcgc
    34741 ctgcaggatg cgggtttcga aatgtcgctc agcctgaaag ccaaatggcg accagaccag
    34801 gacgctctgg agttatgccg tgaacacggt cgcgaaatcg cccgtcagtg ggcgctcgcg
    34861 ccgctgccgc agagcacggt gaatacggta gttaaagaag aaacctctgc cacgacgacg
    34921 gctgacctcg gcccacgaat gcagtgcagc gtctgccagt ggatttacga tccggcaaaa
    34981 ggcgagccaa tgcaggacgt tgcgccagga acgccgtgga gtgaagtccc ggataacttc
    35041 ctctgcccgg aatgctccct cggcaaagac gtctttgacg aactagcatc ggaggcaaaa
    35101 tgagcaacgg cattgtgatc atcggttcgg gcttcgccgc ccgccaactg gtgaaaaata
    35161 ttcgcaaaca ggacgccact attccattaa ccctgattgc cgccgacagc atagatgagt
    35221 acaacaaacc cgacctcagc catgttatca gtcaggggca acgtgccgac gaccttaccc
    35281 gccagacggc gggtgaattt gccgggcagt ttaatctgcg cctgtttccg cacacctggg
    35341 tgacggatat cgatgccgaa gcccatgtgg tgaaaagcca gaataatcag tggcaatacg
    35401 acaagttagt gctggcaacc ggtgccagcg cctttgtccc gcctgtgcct gggcgtgagt
    35461 taatgctgac gttaaatagt cagcaagagt atcgcgcctg tgaaacgcaa ctgcgggatg
    35521 cccgacgcgt gttgattgtc ggcggtggtt tgattggtag cgaactggcg atggattttt
    35581 gtcgtgcagg caaagcggtc acgctaatcg acaacgctgc cagtattctg gcgtcattaa
    35641 tgccaccaga agtaagcagc cgcttgcagc atcggttgac ggagatgggc gttcatctgc
    35701 tgttgaaatc tcagttgcag gggctggaaa aaacggattc tggcattctg gcaacgctgg
    35761 accaccagcg tagtatcgaa gtggatgcgg taattgccgc caccggactg cgcccggaaa
    35821 cagccctggc acgacgcgcc gggctgacga ttaatcgcgg cgtttgcgtc gatagttatc
    35881 tgcaaaccag taatgccgat atttacgcgc tgggcgattg cgcggaaatt aacggtcagg
    35941 tattgccgtt cctgcagccg attcaactta gcgggatggt gctggcaaaa aatcttctcg
    36001 gcaataacac gccgctgaaa ctaccggcga tgctggtgaa aatcaaaacg ccggaattac
    36061 cgctgcatct ggcaggcgaa acccagcgtc aggatttacg ctggcagatt aataccgaac
    36121 gccagggaat ggtggcgcgc ggcgttgacg atgctgacca gcttcgcgcc tttgtggtca
    36181 gtgaggatcg gatgaaagag gcatttgtat tgttgaaaac gttgccggtg taggtgggct
    36241 actgtgccta aaatgtcgga tgcgacgctg gcgcgtctta tccgacctac ggggacgcat
    36301 gtgtaggccg gataaggcat ttacgccgca tccggcaagg gtgtccaaat gcaacacgct
    36361 ttatccgttc tggacttcac ccgctaacca acgcgccgca gcaataaccc cctgccccag
    36421 agacaaaccg ccatcacccg ccggtaaact ctgtggaaag agcaatgtga aatcagcgag
    36481 ataatgcgcc agacgtgcac gcagcaaacg gttatgaata accccgccgc taaataccag
    36541 cgtagtgata ccacgcatcg tggcctgctc acgcatcaac gcggcaaaac cctgcgccag
    36601 cgcatcatga aacgcccacg cgcgttgatt aaccagtgcc tgccagttca gccactgctg
    36661 ccagaaagtg gcgagatcca gttgattgtc cacccgcggc atcgtcaccg gatgcgtcac
    36721 tccgtcgcat gaggctgcga gcgcctccag agcacaagcc gcttcacctt cataacttaa
    36781 cgtggctggc gcacagccca gtgccgccgc cactgcatcg aacaaccgcc cacacgatga
    36841 tgccagcggc gcgttaattc cacgctcaat ggcccgcgcc agtacgctcc agttttgctg
    36901 ttgcacactt gccgtttcag ggtaattctg ccactccggc acaaagcgca ggcactgcgc
    36961 cagcaggttt cgccacggct gcttcgccgc caaatcgcca cccggaagcg ccactgcagg
    37021 caagccgccc aggtgctcac attcgcgata gttcacccgc aggcactcgc cgccccacaa
    37081 agcgccattc tcccccatac cgataccgtc gagcgtcaaa gcaatgacat caccgccatc
    37141 cagcggccac tgatgctctg ccagacacgc cgctgcatgg gcatgatgat gcagtaccgt
    37201 ttgcgtcggc agattcattt cgctcgccca ctggctggag acatagcccg gatgcacatc
    37261 atgcacaatg tattgcggag tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg
    37321 ccactgcatc tggatgccat cgtcacttaa atcgcccaga tgctgactca acaccgcttg
    37381 ttcaccgcgc accaggcaga aggtattttt cagatccgcg ccaagacaca gcacaggcgg
    37441 aacattttta aagcccggag gcaaaaccag cgcatccggc acataccccc gcgaacggcg
    37501 cagcatttcg ccgctttcgc gcaccactga atcatccatc cgctgcacga tgtcgcggtt
    37561 atgtagcaag aatccgtcgg caatgccctg caaatccgcc agcgcctgtt cgttgctgat
    37621 agctggtggt ttaccgctca ggttgccgga ggtcatcacc agcgggcatt gcagttcctg
    37681 taacagcaaa tgctggagcg ggttcgcagg caacatcacc ccgacttcgt taaggccagg
    37741 ggcgatatca tcacaaagct caggtacgta ttttttatcc accagcacaa tcggcgcagc
    37801 gggcgtggta agcaactggc gcgcagcgtc tggtaaactt tctgccactg gcaacatgac
    37861 tgccagcggt ttcgccgggc gatgtttgcg ctcccgcagt gtcgccaccg cgttactgtt
    37921 acgtgcatcg caggcaagat gaaatccgcc aatccctttg atggcgacaa tgttgcccat
    37981 ttttaactgt gcgatagctg cctgtaatgc cgcctcttgt tctgcatgtt caccatgact
    38041 tacccattca agatgcgggc cacactccgg gcaggccacc ggctgggcgt ggaagcgacg
    38101 atcgagcggg tcgcggtact ctttgtcaca ggccggacat agcggaaacg ccgccatcac
    38161 ggtaaacggg cggtcgtaag gcatcgcgcg aataatggtg aaacgcgggc cgcagtgggt
    38221 acagttgata aacggataac gataacgccg ctcgcctggg gtattcattt cggcaaggca
    38281 agcagggcaa gtggcggcat ccgggacaat ttgcgtattc atggtgccgc cggtgctctg
    38341 gcggatggtg aactcggtgg gcagttgtga ccagataaac ggctcacgct cgacgctatc
    38401 aatacgcgcc agtggcggac agtgctgatg caattgaaca agaaacgttt ccgggtcttc
    38461 cagcagccgg acttctacgc catcgccgtc attacataca tcgccgtgaa gatttaattg
    38521 ctgtgccagc tgccagacaa acggacgaaa accgacgccc tgcactttgc cacgaatacg
    38581 cagttggaca ccgcaagatg tgttttttgc cattgagtta ttcccgccat catgaattgc
    38641 gtaacccgcc ctgccggaca cgacagcgtc gtatccggca gtctcaggtc ggcgataccg
    38701 cccgctccgt attctacgaa tatttccggg aattcctttg atgccagaac agttctgtaa
    38761 gatttttaga acatcagcgc cgtacggcgg cgtttttctg cgctcagttg ttcaagttta
    38821 ttacgatcga cacaaatcag cgcatgggtc gggcaagccg ccatacacgc cgggccgtct
    38881 tcacgatggt tgcacaggtc gcatttattg gcttcggctt tgtcagcccg tacattcaga
    38941 cccgcgccgc tgttgcgaat cacaggacgt accaccactt ccatcgcgcc atacgggcaa
    39001 gccaccacgc aggttttgca accaatgcaa cgttcctgca tcacaggaac aaacccttta
    39061 tcacggctga tagcaccatt cgggcagacg ttagcgcacg gtgcatcttc acactgacgg
    39121 caaactgtcg ccgtggaaat gttcacacct ttaatgacat ggatacgcgg taaaaatgtt
    39181 tccggggtca gcgatgcaca gtcctgattt tcctgatgag aaaccacgca cgccacttca
    39241 caggtacggc aaccaataca tttactcgcg tcagcaatga tgaaacggtt catcaaattc
    39301 tccagcaatg acagttaatg cgccgataca ctcacaaatc atgccagttt ttaatttgct
    39361 gttatttaag gaaattaatt tctgtaatgc aggaaaaaag atgtcatcga cactagtgac
    39421 gatgacatgt gatgacaatg tttatcgcga aagggcaatg agtgagtcgc ggcggatcag
    39481 ttttccgcta aaggttttcg gcggtgagaa atccccgcca tcgagcataa aaatcagccg
    39541 tccaataatt tcctgaatca tctcagtcac cggaattttt acgctggaga gcgccggaac
    39601 gatatagggg gcaattgcga tatcatcgaa tccgataact gacacctgct ctggcaccgc
    39661 tacgccgcgc tcgtgcaacg ctttgatcgc acctatcgcc atatcgtcgt tactggcaac
    39721 taacgcgcta aatttagccc ctcgttcgag caacgtttct accccttcgg ccccgctggc
    39781 gggcgtccat ttaccgttag cgataagttt ttcattgagc gcaataccat gctgcgacag
    39841 cgcgtcttta tacccggcaa gacgttcaat gctggtgggg gaatccatcg agccggtaag
    39901 gaaagcaatc tcctgatgcc cggcgtttat caactctgcc acggcgttaa agctggtctg
    39961 tttatgatcg caccagacgc tatggctgct attattgcgc aggcggcgat taagcaccat
    40021 tatcggctga ctgtgcgcgt caatgatgtc atcaatctca tccacgctta aaaaacgcgg
    40081 gtaaatcata atggcgtcgc agcgcaaatc cagcagatac tgaatcgcct ggcgctcttc
    40141 ttcggcgctg tgtttaccat ctgccaatag caactgtcgc cctttctctt ccgccattcg
    40201 cgcggcatga aacagtaatt cactaaaata aatgccgtga taaagcgtgt tggtcactac
    40261 cagccccagc gtctgagtac tcttcgccga cagattgcgg gccagcaaat ttgggcggta
    40321 actgctctct tctaccgcct gaaacacgcg atctttagtc tcctggctga cgtagccatt
    40381 acctgaaagc acgcgggaaa cggtcgcttt tgaaaccccg gcgcgcttcg ccacttccag
    40441 catcgtcgtc atcattttca tccctttaca cgcaatcaac gcagtgtact gcaccgtttg
    40501 ccgattgtcc ttgcgcaatc ggctccagcg gataagacga ggtcgccgtg ccggttttga
    40561 tgactttttt gataaaggta aacatggcga ttccttattt cagcggcgag tttttacgct
    40621 caatgctgta acgctcgagt tctttgtacg gcaccacttt gctcttcttc ttacgcacat
    40681 cgactacggt catgcggtcg gtacaggagt agcaagggtc caggctaccg ataatcagcg
    40741 gcgcatagga aacggtattg ccacgcagca tgtagcgcag ggtcggccag ttcgcgtagg
    40801 tcgcggcacg gcagcgccag cggtacagct tctggttgtc gccggtcatg ctccagtgga
    40861 tatcgtcgcc gcgcggcgct tcggcaaagc ccagcgcgaa acggtgcgga atgtaggtaa
    40921 agccttccac catcagcgga ccgcccggca ggttatccag accgtagtcg atcatattca
    40981 gcgcggtata gacctcgttg attcgcactt tcagacggga aataacgtcg cagccctgct
    41041 cgctgtggac ttccattggc agcaggccat aaccgacaaa cgggtgatcg gcgcgagtat
    41101 cgcgggcgag accgctggcg cgaaccatcg ggccaacgtt actgaaatcg cgggcgattt
    41161 ccgggtccag acgaccaatg ccgacggtgc gttgttccat gttcggcgta ctcagcagca
    41221 catccaccag ctcctgcact tcacgacgca tttgttgcgc cagctggcgg gtctggatca
    41281 tgtcatcttt cagcagatcg cgacgaatcc cgccgatcag gttcaggccg taggttttac
    41341 gcgcaccggt aaggatctct gccattttca tggaggtttc acgcacgcgg aagaactgca
    41401 taaagccgga gtcgaagccg gtaaagtgac aggcgaggcc gaggttgagc agatgcgggt
    41461 gcaggcgttc cacctccagc agaatggcgc ggatcatctg cgcgcgttct ggcacctgaa
    41521 tacccatcgc gttttccacc gacgtggtgt aggcggtgct gtgggcaaag ccgcagatcc
    41581 cgcacacacg gtcagagagg aaggtcactt cgttataacc catgcgggtt tccgccagtt
    41641 tttccatacc gcgatggacg tagaacagac ggtagtcggc gtcgataatg ttttcgccat
    41701 cgacgaacag acggaagtga cccggttcgt cagaagtgac gtgcagcgga ccaatcggca
    41761 cgacgttgtt tttcttgtcg cccagttcgt tgatgaactc gtaggtttca gcatcggtag
    41821 tcggtgccgg acgctgacga taatccatgc tgtctttacg cagcggataa agttcatccg
    41881 gccagtcatc cggcagcacc agacgacgtt catccggcag accaaccgga atcaaaccgt
    41941 acatatcgcg cacttcgcgc tcgccccaca ccgccgccgg aacgcgcggc gtcacggacg
    42001 gatactccgg tttgttggcg tcaacttcga cgcgaaccgt tacccaacac ttggtgccct
    42061 tctccatcga cagcacgtaa taaacggcgt aatgaccatt cagtttgcgt tcgtcgttac
    42121 caaacagcac cgacagccag ccaccctgct tgtagtagag aaactccacc acttccggca
    42181 gatagttcac ctttacggtg acagtcagct gatctttggt ctgccaggcg tggtccagca
    42241 cgacgcctag aaatgcctca ttcagcgcgg cgagataatg ttgacctaat ttttcttcag
    42301 acatgctcaa actctcttta atcacgccgc cagcaaggag acgaacgcta aaaatgcaaa
    42361 gccaaaccca gcccaggtaa tgcgcggagt aatatcaaga cgcagacggg ccatgctgtt
    42421 ttcgaacagc gcgataacca ggacgccgac caccagtttg acgatggcaa tcaccagcgc
    42481 cagcagcagt ccaccgacgg tgaaggtttc catttgtccc cacggaataa acaccccgac
    42541 gaacatctgc aacaccacca gctgtttcag gctgataccc catttcatga cgccaaagcc
    42601 gctaccgctg tattcggaga gcggaccttc ctgcaactcc tgctcggctt ccgccaggtc
    42661 gaacggcagt ttgcccattt cgataaaagt ggcgaacgca caggcgcaga gcgccagcac
    42721 cagcgggatg ctttggctca gcggccagtg ataaacggtg tcggtaatgt tgctgatgtt
    42781 ggtggaaccg gcaacctgtg cggcaaccca cagaccaagc agcaacatcg gttcgaccag
    42841 cacgccaagc atcgcttcac ggctcgcacc gatagcggta aacgggctac cggtatccag
    42901 accggaaatg gcaaagaaga aacgcgcgat ggcgaagaga tacagtaagg tgatcaaatc
    42961 gcccagttgc ggcagcggag aaccgacggt caccaccggc agcgcagtag cgatagtcag
    43021 catgacgccc accatcacat acggcgtcag gcggaacatc cagccagagg catccgggcc
    43081 aacgctctgg cggcccagca gtttgataat gtcgcgatac tcctgcaaca cgcccggccc
    43141 gcgacggtta tgcagacggg cgcgcgccac gcgggtaata ccggagagca gcggcgcaac
    43201 ggcaaataac accagcgcct gaattaacgg atataaaaca ctcattctca ggctcctcgt
    43261 gaaacaataa tcaccaccag taccgccagt tcaaccagcg ccatccggcg gaacagcaac
    43321 gcactacctt cgcactgcca gcccggcact agcgacaccg gattcagcca tttgcgcagt
    43381 ttcagcactg gcgcaaacgc ctgtttaacc ggcatggcaa aaccgtgagc ggtaatcacc
    43441 attgattttt catggtcgta gccgcacacc caggccgcac cgcgggaacg tgacggcagg
    43501 cggtcgcctt tgcaaatcgc cataatgatg aatggcagca gcgggcaggc aatcagcagc
    43561 aatgtgatca ttggttgaga aacggtggtg ttagcaggct ccagaggcag aggtacagca
    43621 gcagagagca tcggcagcag ccacggcgca gcaacaccgc caattacgca gcaaatcgcc
    43681 agtgccacta cgcttacgct catcaggagc ggcgcacagg tggcgttttc ggcttctttg
    43741 gtacgcggcg cgccgaggaa cgtgacgcca tagactttcg ccatacacat caccgccagc
    43801 gcaccggtaa ttgccagacc cacagcgagc agcggcccca gcagacgggc aacaaacgcg
    43861 ccgctattgc tcagtttgaa gaaggattga tagataaccc attccccggc aaacccgttc
    43921 agtggcggca gcgcagccat tgccatcaac ccgactaaca tggcgatgga gataaccggc
    43981 attttcttgc caataccacc gagtttttcg atatcgcgat gaccggtacg gaaccagacg
    44041 ctccccgccc cgaggaacag ta
//
LOCUS       DAZXUL010000024        40544 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZXUL010000024 DAZXUL010000000
VERSION     DAZXUL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40544
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.024"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..956)
                     /locus_tag="ACH3L1_001216"
     CDS             complement(<1..956)
                     /locus_tag="ACH3L1_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFT4076030.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="ACH3L1_001217"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="ACH3L1_001217"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFT4076031.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACH3L1_001218"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACH3L1_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACH3L1_001219"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACH3L1_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFT4076032.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="ACH3L1_001220"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="ACH3L1_001220"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFT4076033.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="ACH3L1_001221"
     CDS             complement(5018..6346)
                     /locus_tag="ACH3L1_001221"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFT4076034.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="ACH3L1_001222"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="ACH3L1_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="ACH3L1_001223"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="ACH3L1_001223"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFT4076035.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="ACH3L1_001224"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="ACH3L1_001224"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFT4076036.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="ACH3L1_001225"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="ACH3L1_001225"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFT4076037.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="ACH3L1_001226"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="ACH3L1_001226"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFT4076038.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="ACH3L1_001227"
     CDS             complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="ACH3L1_001227"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFT4076039.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15311..16546
                     /gene="moeA"
                     /locus_tag="ACH3L1_001228"
     CDS             15311..16546
                     /gene="moeA"
                     /locus_tag="ACH3L1_001228"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFT4076040.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16546..17295
                     /gene="moeB"
                     /locus_tag="ACH3L1_001229"
     CDS             16546..17295
                     /gene="moeB"
                     /locus_tag="ACH3L1_001229"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFT4076041.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="ACH3L1_001230"
     CDS             complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="ACH3L1_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFT4076042.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18267..19166
                     /locus_tag="ACH3L1_001231"
     CDS             18267..19166
                     /locus_tag="ACH3L1_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFT4076043.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19172..21604
                     /locus_tag="ACH3L1_001232"
     CDS             19172..21604
                     /locus_tag="ACH3L1_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFT4076044.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21750..22565
                     /gene="ybiV"
                     /locus_tag="ACH3L1_001233"
     CDS             21750..22565
                     /gene="ybiV"
                     /locus_tag="ACH3L1_001233"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFT4076045.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22717..23982
                     /locus_tag="ACH3L1_001234"
     CDS             22717..23982
                     /locus_tag="ACH3L1_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFT4076046.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24223..25815)
                     /locus_tag="ACH3L1_001235"
     CDS             complement(24223..25815)
                     /locus_tag="ACH3L1_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFT4076047.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26034..26954
                     /gene="ldtB"
                     /locus_tag="ACH3L1_001236"
     CDS             26034..26954
                     /gene="ldtB"
                     /locus_tag="ACH3L1_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFT4076048.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27012..28130)
                     /locus_tag="ACH3L1_001237"
     CDS             complement(27012..28130)
                     /locus_tag="ACH3L1_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFT4076049.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="ACH3L1_001238"
     CDS             complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="ACH3L1_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFT4076050.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28780..28908
                     /gene="mntS"
                     /locus_tag="ACH3L1_001239"
     CDS             28780..28908
                     /gene="mntS"
                     /locus_tag="ACH3L1_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFT4076051.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29180..30763
                     /gene="opgE"
                     /locus_tag="ACH3L1_001240"
     CDS             29180..30763
                     /gene="opgE"
                     /locus_tag="ACH3L1_001240"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFT4076052.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="ACH3L1_001241"
     CDS             complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="ACH3L1_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFT4076053.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31379..31444)
                     /locus_tag="ACH3L1_001242"
     CDS             complement(31379..31444)
                     /locus_tag="ACH3L1_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076054.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31679..32566
                     /gene="rhtA"
                     /locus_tag="ACH3L1_001243"
     CDS             31679..32566
                     /gene="rhtA"
                     /locus_tag="ACH3L1_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFT4076055.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32865..33368
                     /gene="dps"
                     /locus_tag="ACH3L1_001244"
                     /gene_synonym="pexB"
     CDS             32865..33368
                     /gene="dps"
                     /locus_tag="ACH3L1_001244"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFT4076056.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33772..34518
                     /gene="glnH"
                     /locus_tag="ACH3L1_001245"
     CDS             33772..34518
                     /gene="glnH"
                     /locus_tag="ACH3L1_001245"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFT4076057.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34657..35316
                     /gene="glnP"
                     /locus_tag="ACH3L1_001246"
     CDS             34657..35316
                     /gene="glnP"
                     /locus_tag="ACH3L1_001246"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFT4076058.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35313..36035
                     /gene="glnQ"
                     /locus_tag="ACH3L1_001247"
     CDS             35313..36035
                     /gene="glnQ"
                     /locus_tag="ACH3L1_001247"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFT4076059.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36296..38520
                     /gene="ybiO"
                     /locus_tag="ACH3L1_001248"
                     /pseudo
     CDS             36296..38520
                     /gene="ybiO"
                     /locus_tag="ACH3L1_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="ACH3L1_001249"
     CDS             complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="ACH3L1_001249"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFT4076060.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39719..39979
                     /gene="mcbA"
                     /locus_tag="ACH3L1_001250"
     CDS             39719..39979
                     /gene="mcbA"
                     /locus_tag="ACH3L1_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFT4076061.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40244..>40544
                     /locus_tag="ACH3L1_001251"
     CDS             40244..>40544
                     /locus_tag="ACH3L1_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFT4076062.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10098 a        10717 c         9866 g         9863 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
    15121 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
    15181 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
    15241 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
    15301 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
    15361 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
    15421 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
    15481 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
    15541 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
    15601 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
    15661 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
    15721 acgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
    15781 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
    15841 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
    15901 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
    15961 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
    16021 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
    16081 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
    16141 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
    16201 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
    16261 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
    16321 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
    16381 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
    16441 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
    16501 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
    16561 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
    16621 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
    16681 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
    16741 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
    16801 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
    16861 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
    16921 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
    16981 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
    17041 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
    17101 acctgcgtag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
    17161 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
    17221 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
    17281 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
    17341 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca
    17401 gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat
    17461 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact
    17521 tcgtcacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
    17581 gagtaattga ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
    17641 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
    17701 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
    17761 ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg
    17821 tcggaatccc ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa
    17881 ctttcaccac gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt
    17941 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
    18001 gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg
    18061 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
    18121 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
    18181 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa
    18241 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
    18301 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
    18361 gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc
    18421 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
    18481 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
    18541 gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga
    18601 ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg
    18661 gtgaaccctt catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag
    18721 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
    18781 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
    18841 agtggaccga cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg
    18901 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
    18961 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
    19021 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
    19081 aaaaaccgct tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag
    19141 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
    19201 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac
    19261 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
    19321 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
    19381 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
    19441 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
    19501 tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca
    19561 gacggtacag gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac
    19621 cggcatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt
    19681 cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
    19741 gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga
    19801 tatcatgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat
    19861 ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg
    19921 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
    19981 catccagttg attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga
    20041 tcagtatctc tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga
    20101 acacgccatc gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg
    20161 ctccggctcc cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg
    20221 cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat
    20281 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
    20341 aggaatgagt aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat
    20401 gccggcattc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
    20461 gcaggacgct tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg
    20521 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
    20581 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
    20641 ggcaggtaac ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta
    20701 ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
    20761 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
    20821 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg
    20881 caacagcctg gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca
    20941 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
    21001 gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc
    21061 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg
    21121 tggtccggtt ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt
    21181 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
    21241 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg
    21301 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
    21361 aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga
    21421 agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
    21481 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
    21541 cttcaccgcg ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct
    21601 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
    21661 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
    21721 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg
    21781 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
    21841 aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc
    21901 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
    21961 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
    22021 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
    22081 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
    22141 atcgcctgaa acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
    22201 tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg
    22261 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
    22321 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
    22381 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
    22441 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
    22501 atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca
    22561 gctgacctct ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat
    22621 cacatcaaat cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt
    22681 agcatagttt cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac
    22741 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    22801 cttggcgatg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    22861 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    22921 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    22981 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    23041 tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    23101 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    23161 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    23221 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    23281 cgccgccttc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    23341 ctggaacgct ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat
    23401 ctggcgaaat acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    23461 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat
    23521 atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta
    23581 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    23641 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    23701 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    23761 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    23821 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    23881 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    23941 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    24001 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    24061 taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata
    24121 ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc
    24181 agccgtcgtc accccgacag tatcggggtg acggataatc acttactcga tccctttact
    24241 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    24301 aatgcgggtc gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc
    24361 ctgatacagt tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt
    24421 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    24481 ccctttttcc ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag
    24541 caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact
    24601 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    24661 accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    24721 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc
    24781 accgacctcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    24841 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa
    24901 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    24961 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    25021 caactcagca atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcttg
    25081 ggtcgccgcc gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc
    25141 cagatccgcc atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    25201 catggtgctg tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag
    25261 gttgttggtc ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag
    25321 aagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    25381 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    25441 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    25501 caaagcatag atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat
    25561 caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    25621 gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat
    25681 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    25741 gccgaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    25801 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    25861 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    25921 tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca
    25981 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    26041 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    26101 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    26161 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg
    26221 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    26281 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    26341 ttaacagtgc agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc
    26401 tgccgatcgg cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg
    26461 agcataagaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg
    26521 ctgcgggcga accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    26581 atgcactcta catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg
    26641 gcctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg
    26701 agaaagtacc ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    26761 agccagacgg cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt
    26821 ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc
    26881 agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg
    26941 ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta
    27001 tttgccagac cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc
    27061 cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc
    27121 atacgtagcg caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt
    27181 accgcccatg ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc
    27241 acgttactga tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa
    27301 tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg
    27361 ataaacatcg ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc
    27421 gccagcagca caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc
    27481 agcgccgtca gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac
    27541 tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac
    27601 aacagcagga gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg
    27661 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    27721 agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc
    27781 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    27841 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    27901 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    27961 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    28021 ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat
    28081 aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt
    28141 gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc
    28201 cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga
    28261 ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca
    28321 cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta agcattttag
    28381 ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga gcttccccca
    28441 cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg
    28501 cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt
    28561 tttttgctat tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat
    28621 acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt
    28681 aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat
    28741 ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt
    28801 atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc
    28861 gatatggtca acaacaaacc gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt
    28921 acgccgcttc actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact
    28981 ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg
    29041 gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct
    29101 ttcccctctt cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg
    29161 tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg
    29221 ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt
    29281 aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc ttttgctatg
    29341 gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg
    29401 ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca
    29461 ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct
    29521 ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga
    29581 aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt
    29641 tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag atactggcta
    29701 tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca
    29761 agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc
    29821 gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc
    29881 ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt tgatatttgc
    29941 tgactacatt gctgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt
    30001 agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt
    30061 ccagacctgg tggttttcca accagggcca aatcggcgaa tacgatactg ctatcgccag
    30121 catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa
    30181 aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc aagagcactc
    30241 gcaaccgcag ctgattgttc tgcatctgat gggctcgcat ccgcaggcct gcgacaggac
    30301 acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat
    30361 gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag
    30421 cttctctctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt
    30481 gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat
    30541 ttccagcggc gataaagcgc atcgggtgat taaagcccgc cgctcagcca atgacttctt
    30601 aggctttttc tcgcagtgga cgggaattaa agcgaaggaa ataaatatca aatacccgtt
    30661 tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga
    30721 ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc
    30781 gagaggcgga tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca
    30841 ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt
    30901 ttccatcggg ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg
    30961 tttgtaggtc gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta
    31021 gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt
    31081 tttgttgtag tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat
    31141 cacacccagc gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt
    31201 gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc
    31261 tacggaagta cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt
    31321 tttcataacc acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt
    31381 aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt
    31441 ccatacaccg ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac
    31501 gcatgttttc cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca
    31561 atcctggcgc attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat
    31621 tgggtatatc cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat
    31681 gcctggttca ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat
    31741 ggcgtctatt cagggtggag cctcgttagc taagtcactt tttcctctgg tgagcgcacc
    31801 gggtgtcact gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc
    31861 atggcgactg cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct
    31921 gggtgggatg aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt
    31981 ggcgctggag ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga
    32041 tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga
    32101 cgtttcccat gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc
    32161 tatttacatt ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtagcaat
    32221 tggttcgttg attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc
    32281 actctggcac tggtcggtta ttccattggg tctggctgtc gttattctct cgaccgctct
    32341 gccttattcg ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct
    32401 gatgagcatg gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact
    32461 gacacccata cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct
    32521 gacagtacgc aaagagagca aaataaaaga attagacatt aattaaattt acatttctgt
    32581 atggttatgc ataaccatgc agaatttctc gctacttttc ctctacaccg tatttatata
    32641 tcgaattatg caaaagcata tttattccga aaattcctgg cgagcagata aataagaatt
    32701 gttcttatca atatatctaa ctcattgaat ctttattagt tttgtttttc atgcttgtta
    32761 ccactattag tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta
    32821 gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat
    32881 tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacagcgaga
    32941 aaaaagcaac agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga
    33001 ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc
    33061 tggatggctt ccgcaccgca ctgatcgatc atctggatac catggcagaa cgtgccgtgc
    33121 agctgggcgg tgtagctctg gggaccactc aagttatcaa cagcaaaacc ccgctgaaaa
    33181 gttacccgct ggacatccac aacgttcagg atcacctgaa agaactggct gaccgttacg
    33241 caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag
    33301 atatcctgac cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca
    33361 tcgaataaat ccatcgctga tggtgcagaa ctttagtacc cgataaaagc ggcttcctga
    33421 caggaggccg ttttgttttg cagcccacct caacgcactt atttagtgca tccatctgct
    33481 atccccagct gattaagtaa attttttgta tccacatcat cacacaatcg ttacataaag
    33541 attgtttttt catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg
    33601 caccagattg gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc
    33661 acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa ttggcacgat tttttcatat
    33721 atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct
    33781 gtattaaaag tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg
    33841 gataaaaaat tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc
    33901 gataaatatg tgggctttga cgttgatctg tgggctgcca tcgctaaaga gctgaagctg
    33961 gattacgaac tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac
    34021 gtcgatctgg cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc
    34081 tctgacggct actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg
    34141 aaaagcgtga aagatctcga cgggaaagtg gttgctgtga agagcggtac tggctccgtt
    34201 gattacgcga aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac
    34261 gcctatatgg aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt
    34321 ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa
    34381 gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac
    34441 ggcgcgttga aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc
    34501 agtactgaac cgaaataata acgctacacc tgtaaagcgc actggcagtt ccctctcccc
    34561 cctggggaga ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga
    34621 atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc
    34681 tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc
    34741 ggtctggctg gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt
    34801 tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc
    34861 gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac
    34921 ccatttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc
    34981 acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg aagcaggact ggcgctcggt
    35041 ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg
    35101 ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgttatc
    35161 ggcgtggcgg aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt
    35221 gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt
    35281 ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc
    35341 actttggccc aacccaggtg ctgcacaata tcgatttgaa cattgcccag ggcgaagtcg
    35401 tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac
    35461 tggaagaaat cacctccggc gatctgattg tcgatggcct gaaggtcaac gatccgaaag
    35521 ttgacgagcg cctgattcgc caggaagcag gtatggtgtt ccagcagttt tacctcttcc
    35581 cgcatctgac ggcgctggga aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca
    35641 aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagaacgtg
    35701 cacatcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg
    35761 cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg
    35821 aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg
    35881 tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg
    35941 acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt gatcaagaac ccgccgagcc
    36001 agcgcttgca ggaatttttg cagcacgtct cttaataaga cacattgcct gatgcgctac
    36061 gcttatcagg cctacaggat atctggcaac ttattaaaat tgcatgaact tgtaggacgg
    36121 ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg ttgtcagcaa cctgcttaat
    36181 atcccttcct ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc
    36241 ccctccccca gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg
    36301 gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc
    36361 cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat
    36421 cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt
    36481 aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg
    36541 aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc
    36601 agccgccatt atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatatcacc
    36661 ggctccccgc ataagccatt taatccacaa accttcagca atgcgctgag ccatttttca
    36721 atgttagcgg tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgcta
    36781 tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa ctggttgcag
    36841 cttccggcga tgattattgg ggcgtttatt atcgacctgc tgttactggc cctgacatta
    36901 tttgtcggcc aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa
    36961 caaagtttgt ttctcaacgc ctttgccctg attgaatttt tcaaagccgt actacgcctg
    37021 attttttgcc caaacgtggc ggagctgcgc ccgttcacga ttcaggacga gagcgcccgt
    37081 tactggggtc gtcgcctgag ctggttaagc agcctgattg gctatggcct gattgtggcc
    37141 gtgccgatta tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt
    37201 atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc gaaataaaaa agagattacc
    37261 cagcatatgc tcaactttgc ggagcattcg ctggcttttt tcagcctgtt tatccgcgcc
    37321 tttgcgctgg tgtggcactg gctggcaagc gcctatttta tcatgctgtt tttcttttcg
    37381 ttgttcgatc cgggcaacag tctgaaattt atgatgggtg caacggtgcg cagcctggcg
    37441 attattggta tcgcagcgtt tgtttccggt atgttttccc gctggctggc gaaaaccatc
    37501 accctctcgc cacatactca gcgtaactat ccggagctac aaaaacggct gaatggctgg
    37561 ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcagt aatgttgctg
    37621 ttgagcgcat ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa
    37681 accgtagata tcctgatccg catcgcactc attcttttct tctcggcggt tggctggacg
    37741 gtgctcgcca gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc
    37801 agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt gattatcagt
    37861 accatcacca tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca
    37921 ggtgccgggg cattaggtct ggctatctcg tttggttcgc aaacgctggt aaaagatatt
    37981 atcaccgggg tatttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc
    38041 gggccgttga ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat
    38101 accggggcgt atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc
    38161 ggtattggtt cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc
    38221 aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa acgaagaaat tcgcgggctg
    38281 attattggtg aaccgaactt tgccgggatt gtcggcttaa gcaataccgc gtttacactg
    38341 cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc
    38401 caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg
    38461 ctgcctgctc cgggcgcgac cccggctgaa ccgctgccgc cgggggagcc aacgctttaa
    38521 cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag
    38581 cgactctgct tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg
    38641 gtcagagcac gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac
    38701 atcacctgct tcgcgaagcc tttgctctct tcaatcatct ttttgataaa gttgacttca
    38761 ccgccttcac accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc
    38821 agccccaggt tacggcgttt acgctcacta cctgcccgcg ccgcagcggc ggaatcgtgg
    38881 aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt tgtggatgat gccgttaaaa
    38941 atcgccccac tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca
    39001 ctgataatcg cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc
    39061 cagccatatt catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc
    39121 agaatgctgg cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga
    39181 tgaatataat ccgcccggcc cggtactggt gggcagagaa aaccgtcggg gatatcccag
    39241 ttcgctacgg cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga
    39301 ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg tgaggaattg cctgagttca
    39361 ggattgacct gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc
    39421 aaccccggtt tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca
    39481 taacgcgaca gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta
    39541 tggtttatgg caattcatca taaaaatcag cattatgcat gtcattgatt tgtcttattt
    39601 tgtaaaaata tatcgttcaa aaaataagca tttctctgtc aattttttgc gtcatttgca
    39661 tcgcgcaggc gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat
    39721 gaaaaagtgc ctcacactac tgattgccac cgtcctgagc ggaatctctc tcacggctta
    39781 tgccgcgcaa ccgatgagta acctggacag cggtcagcta cggcccgccg gtaccgtttc
    39841 ggcaactggc gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga
    39901 acaaggcgcg aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttaggtac
    39961 tgcaaccatc tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc
    40021 gcctgttttg taatcaaaaa acatcaaata tgaacgcaat gtaaataaat gtatttcttt
    40081 ttcgcgcaat gggtgataga aaatcgcatc aagtgataat gcttatcaaa attattatca
    40141 cttgcacgaa cactatcacg ggattaacag tggcatcgca tccgcagaga tgctttctcg
    40201 tggcagtgaa aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa
    40261 tttccctgcc agacaatttc attcgctcac gttctttgcc ggtctttgta ttggtattac
    40321 gcctgtggca caggcactcg ccgccgaagg gcaagctaac gcggatgaca cgctggttgt
    40381 cgaagcatca acgccttcgc tttatgcgcc acaacaatct gccgatccga aattctcgcg
    40441 tccggtagcg gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca
    40501 gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc ggcg
//
LOCUS       DAZXUL010000025        40446 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZXUL010000025 DAZXUL010000000
VERSION     DAZXUL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40446
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.025"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACH3L1_001252"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACH3L1_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFT4076063.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..2636)
                     /locus_tag="ACH3L1_001253"
     CDS             complement(1899..2636)
                     /locus_tag="ACH3L1_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076064.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACH3L1_001254"
     CDS             complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACH3L1_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFT4076065.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3584..4093)
                     /gene="traS"
                     /locus_tag="ACH3L1_001255"
     CDS             complement(3584..4093)
                     /gene="traS"
                     /locus_tag="ACH3L1_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFT4076066.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            complement(4090..6915)
                     /gene="traG"
                     /locus_tag="ACH3L1_001256"
     CDS             complement(4090..6915)
                     /gene="traG"
                     /locus_tag="ACH3L1_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFT4076067.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            complement(6912..8285)
                     /gene="traH"
                     /locus_tag="ACH3L1_001257"
     CDS             complement(6912..8285)
                     /gene="traH"
                     /locus_tag="ACH3L1_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFT4076068.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            complement(8272..8664)
                     /gene="trbF"
                     /locus_tag="ACH3L1_001258"
     CDS             complement(8272..8664)
                     /gene="trbF"
                     /locus_tag="ACH3L1_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFT4076069.1"
                     /translation="MKEKINDTEPGIKQIEREIERGCDNAKKYFWLFVVFFAAGLIVR
                     NVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLAAASLSPLCEII
                     FGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="ACH3L1_001259"
     CDS             complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="ACH3L1_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFT4076070.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="ACH3L1_001260"
     CDS             complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="ACH3L1_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFT4076071.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="ACH3L1_001261"
     CDS             complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="ACH3L1_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFT4076072.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            9819..10154
                     /locus_tag="ACH3L1_001262"
     CDS             9819..10154
                     /locus_tag="ACH3L1_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFT4076073.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="ACH3L1_001263"
     CDS             complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="ACH3L1_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFT4076074.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(10490..11233)
                     /gene="traF"
                     /locus_tag="ACH3L1_001264"
     CDS             complement(10490..11233)
                     /gene="traF"
                     /locus_tag="ACH3L1_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFT4076075.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11226..11483)
                     /locus_tag="ACH3L1_001265"
     CDS             complement(11226..11483)
                     /locus_tag="ACH3L1_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFT4076076.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11510..13414)
                     /gene="traN"
                     /locus_tag="ACH3L1_001266"
     CDS             complement(11510..13414)
                     /gene="traN"
                     /locus_tag="ACH3L1_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFT4076077.1"
                     /translation="MKEKINTFFRSLNFNYSVKPCNLCAIVFFLSFSVFADSNSDYRA
                     GSDFAHQIKGQGSSSIQGFKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWA
                     TGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEF
                     TNYTCERDTMVEEYCTRTASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPV
                     TGTVESASLSVYAAFFFLNSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLT
                     GSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLTGTECSEPGGTKTGVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGTCLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQ
                     IADQLKQAGQ"
     gene            complement(13650..14030)
                     /locus_tag="ACH3L1_001267"
     CDS             complement(13650..14030)
                     /locus_tag="ACH3L1_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076078.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="ACH3L1_001268"
     CDS             complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="ACH3L1_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFT4076079.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(14674..14979)
                     /locus_tag="ACH3L1_001269"
     CDS             complement(14674..14979)
                     /locus_tag="ACH3L1_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076080.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(15009..16001)
                     /gene="traU"
                     /locus_tag="ACH3L1_001270"
     CDS             complement(15009..16001)
                     /gene="traU"
                     /locus_tag="ACH3L1_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFT4076081.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15998..16630)
                     /gene="traW"
                     /locus_tag="ACH3L1_001271"
     CDS             complement(15998..16630)
                     /gene="traW"
                     /locus_tag="ACH3L1_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFT4076082.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(16627..17013)
                     /gene="trbI"
                     /locus_tag="ACH3L1_001272"
     CDS             complement(16627..17013)
                     /gene="trbI"
                     /locus_tag="ACH3L1_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFT4076083.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(17010..19640)
                     /gene="traC"
                     /locus_tag="ACH3L1_001273"
     CDS             complement(17010..19640)
                     /gene="traC"
                     /locus_tag="ACH3L1_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFT4076084.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(19766..20113)
                     /locus_tag="ACH3L1_001274"
     CDS             complement(19766..20113)
                     /locus_tag="ACH3L1_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076085.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(20141..20359)
                     /locus_tag="ACH3L1_001275"
     CDS             complement(20141..20359)
                     /locus_tag="ACH3L1_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076086.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(20458..20931)
                     /locus_tag="ACH3L1_001276"
     CDS             complement(20458..20931)
                     /locus_tag="ACH3L1_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076087.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(20924..21337)
                     /locus_tag="ACH3L1_001277"
     CDS             complement(20924..21337)
                     /locus_tag="ACH3L1_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076088.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(21330..21551)
                     /gene="traR"
                     /locus_tag="ACH3L1_001278"
     CDS             complement(21330..21551)
                     /gene="traR"
                     /locus_tag="ACH3L1_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFT4076089.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(21686..22201)
                     /gene="traV"
                     /locus_tag="ACH3L1_001279"
     CDS             complement(21686..22201)
                     /gene="traV"
                     /locus_tag="ACH3L1_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFT4076090.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(22198..22518)
                     /locus_tag="ACH3L1_001280"
     CDS             complement(22198..22518)
                     /locus_tag="ACH3L1_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFT4076091.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(22505..23095)
                     /gene="traP"
                     /locus_tag="ACH3L1_001281"
     CDS             complement(22505..23095)
                     /gene="traP"
                     /locus_tag="ACH3L1_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFT4076092.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(23085..24512)
                     /gene="traB"
                     /locus_tag="ACH3L1_001282"
     CDS             complement(23085..24512)
                     /gene="traB"
                     /locus_tag="ACH3L1_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFT4076093.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(24512..25240)
                     /gene="traK"
                     /locus_tag="ACH3L1_001283"
     CDS             complement(24512..25240)
                     /gene="traK"
                     /locus_tag="ACH3L1_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFT4076094.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(25227..25793)
                     /gene="traE"
                     /locus_tag="ACH3L1_001284"
     CDS             complement(25227..25793)
                     /gene="traE"
                     /locus_tag="ACH3L1_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFT4076095.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(25815..26126)
                     /gene="traL"
                     /locus_tag="ACH3L1_001285"
     CDS             complement(25815..26126)
                     /gene="traL"
                     /locus_tag="ACH3L1_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFT4076096.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(26141..26500)
                     /gene="traA"
                     /locus_tag="ACH3L1_001286"
     CDS             complement(26141..26500)
                     /gene="traA"
                     /locus_tag="ACH3L1_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFT4076097.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(26534..26761)
                     /gene="traY"
                     /locus_tag="ACH3L1_001287"
     CDS             complement(26534..26761)
                     /gene="traY"
                     /locus_tag="ACH3L1_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFT4076098.1"
                     /translation="MIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            complement(26881..27528)
                     /locus_tag="ACH3L1_001288"
     CDS             complement(26881..27528)
                     /locus_tag="ACH3L1_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TraJ family protein"
                     /protein_id="HFT4076099.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFSCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(27720..28103)
                     /gene="traM"
                     /locus_tag="ACH3L1_001289"
     CDS             complement(27720..28103)
                     /gene="traM"
                     /locus_tag="ACH3L1_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFT4076100.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            28424..29026
                     /locus_tag="ACH3L1_001290"
     CDS             28424..29026
                     /locus_tag="ACH3L1_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFT4076101.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NGICEAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(29323..30144)
                     /locus_tag="ACH3L1_001291"
     CDS             complement(29323..30144)
                     /locus_tag="ACH3L1_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFT4076102.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(30255..30551)
                     /locus_tag="ACH3L1_001292"
     CDS             complement(30255..30551)
                     /locus_tag="ACH3L1_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076103.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            30851..31147
                     /locus_tag="ACH3L1_001293"
                     /pseudo
     CDS             30851..31147
                     /locus_tag="ACH3L1_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(31466..31591)
                     /locus_tag="ACH3L1_001294"
     CDS             complement(31466..31591)
                     /locus_tag="ACH3L1_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFT4076104.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(31533..31682)
                     /gene="mok"
                     /locus_tag="ACH3L1_001295"
                     /pseudo
     CDS             complement(31533..31682)
                     /gene="mok"
                     /locus_tag="ACH3L1_001295"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            complement(31861..32623)
                     /locus_tag="ACH3L1_001296"
                     /pseudo
     CDS             complement(31861..32623)
                     /locus_tag="ACH3L1_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(32620..33054)
                     /gene="psiB"
                     /locus_tag="ACH3L1_001297"
     CDS             complement(32620..33054)
                     /gene="psiB"
                     /locus_tag="ACH3L1_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFT4076105.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(33109..35067)
                     /locus_tag="ACH3L1_001298"
     CDS             complement(33109..35067)
                     /locus_tag="ACH3L1_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFT4076106.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENNTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            complement(35131..35364)
                     /locus_tag="ACH3L1_001299"
     CDS             complement(35131..35364)
                     /locus_tag="ACH3L1_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFT4076107.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            complement(35420..35941)
                     /locus_tag="ACH3L1_001300"
     CDS             complement(35420..35941)
                     /locus_tag="ACH3L1_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFT4076108.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            36240..36677
                     /locus_tag="ACH3L1_001301"
                     /pseudo
     CDS             36240..36677
                     /locus_tag="ACH3L1_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36763..37326)
                     /locus_tag="ACH3L1_001302"
     CDS             complement(36763..37326)
                     /locus_tag="ACH3L1_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFT4076109.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(37373..38734)
                     /locus_tag="ACH3L1_001303"
     CDS             complement(37373..38734)
                     /locus_tag="ACH3L1_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFT4076110.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(38786..39016)
                     /locus_tag="ACH3L1_001304"
     CDS             complement(38786..39016)
                     /locus_tag="ACH3L1_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076111.1"
                     /translation="MTISIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(39983..40174)
                     /locus_tag="ACH3L1_001305"
     CDS             complement(39983..40174)
                     /locus_tag="ACH3L1_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076112.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(40171..>40446)
                     /locus_tag="ACH3L1_001306"
     CDS             complement(40171..>40446)
                     /locus_tag="ACH3L1_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076113.1"
                     /translation="VMQRLDDAFVYGACDRVVSDIVNELMEEKRASRHVTVPAVMLEK
                     VMALAGSEMKRLYAVGSENGGDGDAFVREELEAMDVVLQALDGETMS"
BASE COUNT         9758 a        11093 c         9564 g        10031 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaggcaac ctccggttcg aagaggctgg ccatctggcg
       61 accttctgct tcccttgcag caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgtc tgatatttca gtgagagttt
      181 tactgccgga tacggtccgg gcagagtgac ataacaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctggcgctc catctcctta ccggtcgata ccccgtcacg
      301 cacggggtca gcaccgtagg aatactgcag actggctttc aggtgctctt tctcaccaat
      361 ttcaccggca gcaaactctg caatctgatg gctcggggag cggaaaaagg cacgggtatt
      421 cagcacatca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagcctcc gaacttacgg gcttccggca ggatctccac
      541 caggtccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggaaaccac
      661 cggcttcagg gaggcatgag tgtcggcatt tgacgagata aacagccagc cgtttttctg
      721 atcttcccgg acaccccgca tccagtcacg tatggtgaag ggttcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttcg attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcagatacg ccgcttccgc aaaaatggta cgtcctgatc cctgccagaa
     1021 cgggtcctct ttcgttccca tcgggatcag cgtgttcgcg acattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccaggc ggcacagcgg gcatccagcg gattcaggat
     1141 cttatcgatg gagggatcat agtaactttt aacaaactca cacgaacggt cataaatcac
     1201 caccatgtcg ccacgtttac gggcatagtt ggcaagacgt cgaataacct ctgacttacc
     1261 cgtcgacact gttccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 atcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtaatcagg caaataacca gtgccacaac
     1501 tgtagccaga acgaatgcgg accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctga gcagcgttca tccggaaggt tttgccgtaa tactggatct catagaccgg
     1621 ctgggatttt attaaatccc gcatgccttc cagtgacgta caccaccagt aaatacagcc
     1681 gttgataaac gtctgccagc ttatttttac ccataagacc agaccaatga ggatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatgcccg cctgcagtcc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccatgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacaca ggacgtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaaa tcgcccaggt gatattctcc gcctggtggc catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggaaac ttcagccagc
     2281 tttctgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt tgtcagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttgcaggc
     2581 ttctcagtaa attgattatt ctcaccaata tattcagcga tattgtctga tgtcataatg
     2641 actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc
     2701 acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt
     2761 tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga
     2821 tcagcctcct gacgaggttc agagaatatt tgcgattgat ttggccagct ggtcttcgag
     2881 aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaga ccacacgggt
     2941 ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac cggatgtgcc
     3001 ctgacgcagg gcggcaacat tatccgttgt caccgttgcc ttagtacgct ctgcaatctg
     3061 cacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac
     3121 cagaccagca gcaaggccca caccgagtgt ggcaccggca gaatttgagt tatagccggt
     3181 aataccggca cctaacgctg caccaacagc tgcgccttca taaccacggt tcagccatcc
     3241 ctgagactcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta
     3301 ggctgcatcc ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt
     3361 gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg
     3421 ttcgctggcg ggtttaagcc agatggtctc actcatctga gtcttcacct caaggttacg
     3481 cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg aactgaccag
     3541 tgcaaccatc atcatttttt ttgttttcat ccagaatatc cttttaacta acagactctt
     3601 ttttgaacag acctattact gagactattg cagagagatt ataacgattt atatctatag
     3661 tataaataaa ggaaataatt acaaacagag ttattgataa aaacccaaat aagaacgtat
     3721 gagcaaagga aactattgaa aaaacaatca caaacgacaa gatataagtt ttgttcttat
     3781 catacataaa agatataact ttcgactcat ctctgaattt tttagggatg gcaagataca
     3841 acatcattcc attagtaatg gcaacaaaaa ggataaaacc cattacgcca ctaaaacata
     3901 tatcaaatcc agcatctcgg atatcaactt tatctgccag caaacgaaga aatgtcggcc
     3961 ataataaaac ccataaaaac acatacaccc ctggttttat gacttgtaat aatggcggaa
     4021 tattaactcc ctctacagag agtacatcct ttagtttctg tgtctccgtt tttatatcat
     4081 agtgagtgat cataatggcc ccttcagtct atccttttta tcatcaacca tttttttcac
     4141 agcctcttct gatggctcag gtattatctt ttgactttct cgctcttcat tatatttatt
     4201 attctgagat aatgcctctg ttttatgatg attttgcagt tcagaatact gccctcttac
     4261 aatattttcc tcgccacgga tactgttctg agtatctccg atattgccac gatattctgt
     4321 gaccatatta tcaacctgat gttttacatc attacggata ttactgtcct gcgttctttg
     4381 ctcaataatg gcctgatgtc cctgatggtc agcaataata tcctgactgc caccacccga
     4441 aggtacgctc tccattcctt taccaatatc gccacgggat tcccgccagg cattatcaac
     4501 accaggctgt acctgttcct gcacaaaaaa ccaggccatc gcccgacgac gttctgcaat
     4561 ctccggcgaa ctggtatttg tcagaatagc ttctgcatcc tgcggtgcgc gtttcatcac
     4621 atactgtgca aactgttgcg acaggtcttc actcatctgc ccgctcatgc tttcagtacg
     4681 ggaggccatt tccgcatatt catggctgcg ggtcatattc gtcgtgtact ggtcgtaact
     4741 ctgtttcgct gagttcaggg ctgcagacaa ctggtccacc cgggagtcag cattattgtc
     4801 agtatggctg ccggactcac tgaccttgcg actggtaaag taatcgctgg cttctttaaa
     4861 gtccttactg gctttagcgt caatatcatg gcgagcatca tgggtagctc tgctgccact
     4921 acttgcagaa tgtgaatcat catccgttcg atcaagactt cccttaaacc cgcctctgac
     4981 accaacccca ccaccaacac caagaattcc aggtttaatc ccccattcag catgagcatc
     5041 tgcatatccg ccatacgaga acttagaacc tgttgatgcc agttcctgag tcgcctgctc
     5101 attactgata ttatgcgcct tcgcatagct ttccactgca ctgcgcatac ggctggccat
     5161 catggagtcc tgtgcactca tggtgctgtc agcgccaccg gtgacagaat cactgctccc
     5221 ccggttagaa ccaaactgac tgagcgtgtt ccaggcactg gcaatactgc tgctgaaacc
     5281 atgcagggca ctttctgctc tgttcgacgc ctcccgggcc atttcctgtt gcgccgctgc
     5341 aatctgacgc gttgcattga taccgaccgg taaacgggac atcgctccgc ttgcatccat
     5401 caccatatta ccgtcacggg tctgtgtggc agttgcgcca ctgccggtct ggtacatcat
     5461 ctgaccaaac gacgtggtgc tgttggtgct ccagctgaag ccgttcacgt tttccgtctg
     5521 catgttgccg taggagtaat taccgtcaac cacactgcct gctgcactgg ccgtcggact
     5581 gatagaggaa gaggcgaagt ggctgtacac gctggaaaaa cctgcgccaa ggccttttac
     5641 catcatccat gacaacgggg ggatcatggc tgaaagatac ccggcagtag aggccaggtc
     5701 agagtatttc agctgtatct gagagagttc agacagcacg accggcgcac cattcatctt
     5761 cgcatagaat gtcatggcac tgttcagaat ggcatacaac aacggccagg tctgcagcca
     5821 catcagggca aacacatagc ctttcaggac ggacagtgtc agtttgttga agacagctgc
     5881 cagtaccaga agcgggaaaa taccaatggc aattcccgtc aggatagtct gtaccattgg
     5941 caggtttcgc attgtcacat gaccgataga gacatgcgcc agacgttgct tctccatcga
     6001 tgacgtggtg gccagattca ccaggctggc ggtgtcacca ttacgggcag cattactggt
     6061 gataccctct ttcagggcat ttattgtgac gttctggcgc ataatctggc tggcagactg
     6121 actggagccg tagaaataac tgtaactgtc actgaccagt tgtctgaata acaggtccgg
     6181 gtccggtctg ccgccaaata tctgctgcac ataataatgc caggtcttgc ctcccgtctt
     6241 tgtatcgaga ttcagcctgt ctttcagcgt gaccgacgca tccttacagg ttatgaaatt
     6301 attattgttg tcataaacgc cccgtagcgg gctgggtcgg gaaaaaatca gcgtgtaggg
     6361 gtcagcggag gccatcagat cttccagcgt gtatttatga ttcaggtaaa tatcacccag
     6421 cacgcagttc tggacgtagt cctggaacag attgatgatt tccggattcc gggacaggaa
     6481 atcggtgctt ttcacaatca gattcgcccc gaacagcatg cctgtcttgc tgtaggtgac
     6541 gctgtccggt tgggtgaata tcatctcgta gctgaccacc atcgcatgcc cgatacgggt
     6601 cgtaagagaa agtggcatcg ccagaccgac cggaacatta tccacccgat ggactttgac
     6661 caggtcactg ttatcaataa tctgcaccga tgtgcggaca ttaaccagca ggctgataag
     6721 cacaaacacg gccacccagc ccagcaaatc catcacgtta tgccgctgta cccacataaa
     6781 agccacagca acaacagaca gggtgagcgc gattttttca attgaatccc aggtccgggt
     6841 actcataaat gcagcaatgg cgttcaggtt attccgcaac cactcaccac cggcaatcac
     6901 ataaacttca ttcacagtga attccctccg aagtgatagt tgttctggta acggctgagc
     6961 atacgggcgg aaagctgctg acgcatgtag ttcatctgac gttcgacaac cagcagcgca
     7021 tcctgctgga cctgtacctg actctggaaa atggcaatct gttgctgtgc ctgattcaga
     7081 ttctccagaa cattgtccat cacagcctgc ggatagttcc cggtggcaat catggcccgg
     7141 gcctgctgaa gcagttcctg aatgtactgg agcatgatgt cgtagccgat atagtccgtc
     7201 agctggtaaa cgacgctgtt ggacacaccg agcatctgcg ggtcaatcag atatttaaaa
     7261 acgggaatgg tggtgctgga aataaacccc tgttcccggg cgtcaagcgg agtgtcactc
     7321 actgctttat tctgaatact ggtgagcagt tttgtaatct gtgacttcag ggctttatcc
     7381 cgggcgatgg tcacatttgc atccgccacc accttcagac acttgtccga atcgttgcag
     7441 tggtaaattt ttgccgttcc cccctccata atcgcccgga ggatatcgcg gtcagtggta
     7501 cgcgccggca ggggggtaat ctcgctgtct tccccaaaaa tcagcgtacc ggtgagcgtc
     7561 atgataaatt ctttcaactc tttgttgccg tcaaacattc tgtttttttt cagcgcctcc
     7621 catatgatat tctggttttt cagcacccgc tccttgtcct tgtcgctggc tttgtcacga
     7681 acagatgagg actgccctcc gaccgtgcac ccctgacgag atgcggccca gtcggcgaag
     7741 atattgcttt cactgccaat gtcctgacaa actttctgct gagattcctg cgtctgaggg
     7801 aacagccccc ccacaagccc ctgtgccacc tgacaggaac tgaggttcat actgttaatg
     7861 tcacttgcca ttttctgcag gaagtctttt gcggttttta tttccggcac cgttgtctgc
     7921 agggcaaggt caaaaaagta accggcagca ttgctcataa tctgcttaac aaaacgctgc
     7981 aactgatcgc cattaataaa actgaacgag ccgagatagg cgtcgatgcc gccacatccg
     8041 gcattgatat ccggcagcgt catggaaatc agctggatat ttttgacctg ggtgcgggca
     8101 tacagggagc caccataggc atacccggcg gcctgcccct gccagacgcc cggctgtgtg
     8161 gtattggagg cgaagcccag cttgttaaag aactgattca tatcgctgtt cacatccgct
     8221 gacgctgcgg gtgtgaccgt cagtaaagcg gcacaaagaa ccagaagagg tttaatgcga
     8281 agcattattg ctgccctccc ttaatgtgtt ctcccggcgc atacggcgtt tcagcaggaa
     8341 tatccggaca cccccaaata ttatttcgca caacggcgac agagaagccg cagcaagaaa
     8401 accagctgca agcgcatata acataatcgg aatgacatac atcagggtgt tccacatata
     8461 acgaaaatta tttaattccg ggtcagcttt ccacgaatca ataccggcac tgaaaaaatc
     8521 atgcataacg ttccttacaa tcaatccggc agcaaagaaa accacaaaaa gccagaagta
     8581 tttttttgca ttatcacaac cacgttcaat ctccctttca atttgtttga ttcccggttc
     8641 tgtatcattt attttttctt tcatcataag ttccctctga tatttctgat gattcatgca
     8701 gacaggtcat gcggctgtaa tatatttcag acaccgaaat ccatcccaca attgccccca
     8761 caaaaaacac aacagaaaca gaaaaggtaa gacacagcga aacgacaatt tccacagggc
     8821 tggcgggata aaaaagcccc tgacggataa aggggatatt caggctgacg attattcccg
     8881 tcgcgatgcc ggatattccg gcaatgagta acaccacctg cttatttcgc acctttttcc
     8941 tctccgtata tctgcagaac ggtatccatc cgcgccataa aactggcggc atccgttgcc
     9001 ccctgcaaaa gcggtaatgc ctcaagtgtg ttgacgttga ccaggaaggt ggtcggtgtg
     9061 gccaccggga tattcgggaa gaaggtctgc atcacgtccg gtggcaccgg taatgcttcc
     9121 ggaaaggcag tatccccctg accatccagg gtgtagggaa aaacggaaaa gccgtactgc
     9181 tgtgccagct gcttcagtac cgggtcaaac tggtgacagt aagggcaatg cccctgcata
     9241 aacagcacca ccttccagtc ggccaggtta acctgcctgc cgttgctgag gcggaaccag
     9301 cggggagccg gcttttctgt tctggctgat gtgtctgctg ccggttgtgc aggctgcacg
     9361 gccattccct gcggattcca gagtcgttca atttcatccc gggtggaggc ctgcacacct
     9421 gtggccatca tcaacaaaat gaccggcagt aatttagtga gagacatgtc cgccctcctg
     9481 ctgcccttcc atcatggcct gttgcctggc ttccagtgcc tcacgctctt ccaggctgac
     9541 acgtgcaatc catgcatcca gcacctggta tgcgccgaac agcacaagga tggccgcaat
     9601 caactccgcc agaaaaaaag ccatccaggg tttgctgtaa acaagacggg cgacgatatg
     9661 aaaccagaca cccagggaaa atacccacat tcccgtaata tcaagccggg gtaaagagaa
     9721 tctgcgttta cttatcatgt taaactccgg ttattattta catgagagta ttcatgtctg
     9781 taatattcgc gtttgtgtga tattaatcag aggtacaaat gaaaaagtta tcttttaaag
     9841 aaaaactgga aatagtgaga aacatcataa gagagtcatt atcaggtaat gcggcttttg
     9901 ctttactggt gtattcatta ttgcactccc tccccattaa aacctttgct gatttatttg
     9961 tgacgatact gttatttata gcgattatcg ctgttgtgat atggctattt ctaatcatct
    10021 atatttattt ttgtgagtta ttcagaagtc actggatagc agtctggttt atcctctggt
    10081 ctgtggtaat caacctcact attggatttg gcatttatga caaattagca ccagtcattt
    10141 cactaatgaa ataataaaaa accacatcac tccagaccat atggctgaca acataataca
    10201 aacaaacatc cttctgaaac tgtcaaactt cgtgataacc ggtatggtac tgactgccat
    10261 tgaaacaacc ggtacggcac actgattcat aaccgcacca caaagaaaca agaaattatc
    10321 tggcagcata catacactta tgacatttac tacaaagaag acaaacgagc tcagcagata
    10381 aactccttta ttatttggtc ctgtaaagaa ataaccaaca acccccacga taaacaggct
    10441 ggcacctgta aaaaaacaca aatcccctgt atgcatatat aacctttggt taaaaattcg
    10501 gtttaaaatc ttcagaaacg ttcaggaact gttttgccag gtcgtcctgt gaaataaagc
    10561 cgtatgataa cgggcgaaca ctgccctgtt tcgggtcaac cagcatcatg gccgggaaat
    10621 atttcacacc gaggcgctgc gcctgcccct ggtcagtccg ggaatccggc aacagcggat
    10681 taatcacgcc atccacggaa acgggaataa cactcagacc atacgtatcc cggaagccat
    10741 taatgacctg cgccagttgc ccgtcgatgg ggtcctgccc ccggtaaaaa aacatgatgc
    10801 cgtagtgttc agccagtttc gcaatggcct gtcgctgctg cgcctggtct gccgccagct
    10861 ggttccgtac cgtgccgttg taatggctgt actgcaggtt atagtccagt tcgggatgtg
    10921 ccagcatggc ctttttagca ctcatggtga aaagcccggc ctgctgagtc cagtaattct
    10981 gcagccggaa atatttaaca aaattatcca cgccgggata cagaatggct tcgtacagcg
    11041 cccgctttgt tgccgtctgc agcgcggcca gtttctgcat aatatccggc tcctgacggg
    11101 gggctgcagg tacaggttta ttttcttttt ccttcggatt tattttctcg ttataccact
    11161 gccagcctgc atctttccct gacgccggaa aaataaagca gcagagtaac agtggcagta
    11221 atgctttatt catcttcatt cctttcatca ttttttcttt catcatcttt ctgcaggcca
    11281 gtcagggcag aaaaacaaaa atacagaata aacaggagca caccggagta aaggataacg
    11341 aaagcagcag caagtatccc gtctgccgta gtttctttta ccgtatccca cgaccagata
    11401 attaccggag aaatgacaat cgctgtaatc gaaagccgga taagaaaatc aacaagacga
    11461 ttagaagtga atataacttt catcatttcc cccggttatt ttccatacct tactgtcctg
    11521 cctgtttcag ctggtcagca atctgctctt tcactttctg cgtcagaacg ccactgtccg
    11581 ggattttctg gttattcatc aggtcttcgt agaagttagt gaaatccagt ctgtcgaact
    11641 gaattttctg cagctcatca accgtaatcc cccgacagtc aggatgcttc gcactgccaa
    11701 acccgatacg caactgcccg ttacgcccct gctgctggac tatctgcgcc agcttcgaat
    11761 caaactggca gtagctgcgc tttttctgca gacagacacc cagcactttc cgggagcaga
    11821 actcgcccac actcaccgtc agtttgttgt cctttgcctt tcccagcgcc ttttcttcac
    11881 tgttgcactt cgccagcccc acatcctgac cccagccgcc atccttacag cagttactga
    11941 agccggctgc ggccttacgg cagaatttcg cctcacccgt gaaagccctg acatccacac
    12001 cattcagggc ggcaacatcc ttacctgcgg cggcaagcgc agccagctgt gatactgctt
    12061 caccaaaatc actgtttttt ccgctctgtg ccttatcgca ttcaccgtca aggcagaaga
    12121 catcaccacc acagaccatc acttttcctg atgttctgga ctcgcaggag tacgtggcgt
    12181 actcatgcag acaggttccc tcatcggaat aaaacgcgca ctgacgtgag gccagcgtac
    12241 aggccggatt atcgacatat ttctgacagg tgccgttatc tgcggactga gtgacatatt
    12301 tatcccggta tgcccagcat gcctgtgtga tgttccaggg tttaccttcc atcactccgg
    12361 ttttcgtacc acccggctct gaacactcag tgcctgtcag cacgccatcc gctttattaa
    12421 acggacaact ctccacccac tccacccggg gaacccattc tttatcacgg accttcatcc
    12481 tgagtttcag cgtaaaggtg gaagcaccac tgacaagcga ttcatagact ttcctgtcac
    12541 cattcccgtg cgggaggcaa ttaccgttag cagtacagcc actaccggtc agaacctgcc
    12601 cctgtgtcac ctgcaaacct gtcgccccgg acagggggta agtatccgac tgcccgtttg
    12661 ccaggccgac attaaaggtt gtattcatga aggtataacg ggagttcagg aagaaaaaag
    12721 cggcgtatac agacaatgat gcactttcca ctgtaccggt taccggagcc gtgacactga
    12781 acaccagttt cagcccgttc atactgaacc ggaactgtga gtgcggtatc gtcacttcac
    12841 gatattcatc cgtgtatttc cagtccccgg tgatactggc agtccgtgtg cagtactcct
    12901 ccaccatcgt gtcccgctca caggtatagt ttgtgaattc actgcggttt atctcctgcg
    12961 cactgcactg ctgtccggta tttcccacaa tgctgtcagc cctgttcacc acatccctgc
    13021 cggtctgaat gaacggtgca tccggtgaca gaatgtcttt cggcttgttc atgaaggact
    13081 ctgtgatggt ttttccggtt tcaccggtcg cccattccgt ggtgccgtca tttttcaggc
    13141 cgccatcccc gccggcagtc acgccaccgt aatattttgt ctcgtccgga ttcgcgttat
    13201 agccggggat actctcctgt ggcttaaagc cctgaatact gctgcttccc tgccctttga
    13261 tctggtgagc aaaatcagaa ccggcccggt aatcactgtt gctgtcagca aacacagaaa
    13321 agctcagaaa aaaaacaatt gcacaaagat tgcacggctt tacggaataa ttaaaattca
    13381 gagatctgaa aaaggtattt atcttttctt tcataacctg aaccatactg gagaatgaag
    13441 tgatgtcatc tcaacttcag aactgtgttg attgtggtaa agcattaagt cgaacagcca
    13501 caacgtgcgg gaattgcagc tcaacggacc cttttggaaa aaaacggcgc agcgatcgct
    13561 tttaccagtg gtttgtaggg ctgtttatta cggctattat tattgctggc ggattatggt
    13621 acatggggct tcttgacgta ccgggattac tactcagata acgacttcag taacgatatc
    13681 agtgttctga aatcggcaca taacgagtga cgacaatacc ggttatacca gtaatgaggg
    13741 gtgtcgggct gaagcctgaa acagagctga aacttactgt taaaagcccc cttcaccgac
    13801 catccccatg ccagacgtgg tattgctgat tcagcatcct gccgcagtcg ctgctcaaga
    13861 agggcagcca tttttaccgg acaggcaaag gcaatataag gaggcagctt tgtaacaggg
    13921 aaaccatgtc cctgacagga agcatatgtg cggaagccgt gaatgttcat cgcggccacc
    13981 agggcacgga tctccggatc aatctgataa tacccggcag gtaaagtcat ttttgtttat
    14041 ctcccgggtt attcagcaac gcccgagcca cctgccggca gtcccccgtg gcggccactt
    14101 tctccagcgc ctggccgacc cgcaggttac ctcggataat gtcgtatccc tgactgcaga
    14161 acaccaccag tgccggtaca ctgcgaatgc cgtactgtga aaacagcgtc gggtcgatct
    14221 gaacaccatc agtcgcgccg tctttcacca gggacagcac ggcttcagcg gtggtcttca
    14281 ggtcattatt caccatgccc cgcagtgtgg cgggtatacc gtagtgccgg gtttcaccca
    14341 gcatccgttt cagcccctct tcgggaatgg aaaacgacac aaaatacagg gcaccctgcc
    14401 ggggtttccc gtcctgcgaa gcctgctgtt tacgaaccag ctcatccaga aaatggttat
    14461 ctgaacgctg aagggggttt tccagcacct gtttctccgc ccaggctttc agctgatggt
    14521 caggtttctc tcgcagttgt ctgcttaaat tttcctgctg cttcaggaac tggcggtttt
    14581 caggtgtgtt cacattttct gaagcatgaa cagcaagatg catccctgct gtcagagcaa
    14641 aaagtgcaaa tattttccgg ttcatgatat ttctcacttt ctgctattta caggcatgaa
    14701 ctcatcagaa aaaagataat tgagaaaaac agccataaac gcacatgtaa atgtcacctt
    14761 cagatataat ctgacaagtg tcacttgttc tgatatatct gccttcttcc attcatgaat
    14821 aatttcagcg agaaaactgt caggtgcaaa ataaaataat ataacgggca ccattacaag
    14881 accaaaagga aataatttta atgttctgct tatcagcctg agcagcacag cacttatttt
    14941 cctgaggtgc tgaatatatt tactgtccat tgttgccatt ttcatttcct cttattcatt
    15001 aaaatgattt acaggaatac acagttacgt ttacgccaca tcaggtagcc gaagtttttc
    15061 tttgtgttgg gcggattttt tcccgtctcc cagcgcatca cactgcgccc gaacggatgg
    15121 cactgcccgc tgtccggata catattcacc atctgataac gccagcgttc tttgggcagg
    15181 attggggacg gatattcatt acagacggcg ttatttttcc cgatggtttc cataatcatg
    15241 ccctgacggt gtagtttgaa cgccatgcgt tcactgacca gcagggagga ctgcaacgga
    15301 ctggactcat tactcaccca gccattgaac gggtacatac ttccctgcga accggcacac
    15361 cagaacagaa catcgagagg catattaaag gcgctggcaa tggcatctgc tgcacaggcc
    15421 ccctgtgcta tcggattggc aaagatgaca gcttccggat tgagaatggt ggtcaggctg
    15481 ctgtccgtcc aggtggggtc aatttcagaa agataagcga tatccaggtc accaccttcc
    15541 agacagccca gcgatgtgat gatgttcagc cagtacgtca gcgggtattt gtaccagtga
    15601 acgtgataga acgccccgtt cacctgcttc tcatcctttt tcgccgttcc ctgtgccgtt
    15661 ttaccaaaag ccggcaggct gaagcccagg ttaaccatgc atcccggtga ccgggtgacg
    15721 tccgtcagcg ccatcggctc ccagtaacca atggccagcc cgatacgcct gaacagcggc
    15781 ggcggtgccg gacaaatctg aatgggcatc gacgggttcg ccgtgtcggg gaccttaccc
    15841 tgactgactt tgacactgcc cagcgagagc gggaaaatac agctccagca gatatccgtg
    15901 atcgggttta caaaacgccc ctcacaggca gaatccgcag acgctgcagg gacatgaccg
    15961 gcgagaagga ataacatcag cagccacagg cttcgcttca ttttctgccc tcctctgccg
    16021 gaataaattc caccttcagg aagcgatcgc cgggaacggc gctcacccgt gccgggacct
    16081 gatcaatgcc gaggcgctgg cagagcacac cgttctggtc aaagtaaaca cggctgtcca
    16141 gagacttctg catctccggg atactgccct gcacgaggat aattttgctc tccagtgtgg
    16201 gcggcgtctg gcgtttcatc caggccacct gcgccggatc atcgccattg atgaaataca
    16261 gcgtctggtt aaaaggcaca tactgcagcg gattcatcac ttcaccctga cgggcaaaca
    16321 cccgcccttc gttatcccgg atatccgcag ccagtctgac ggacgggtca aacaaccggc
    16381 tgccgtattt ctccgtgcgt ccgataccgg gcacggcagg gggccgcaga ctgttgcgga
    16441 tcacccgctc cttaaaggca tccattttcc ggcccatctc accggactgc tccagtgccg
    16501 tcaggcgctg catgatcacc gtcagcatgt ccggctcctt taccggccac agatcgcccc
    16561 aggtgccaag atcggcggcg gccacactct gcccccatat cagcagggca atcagccccc
    16621 ggcatctcat ggttccgctc tcattcgccg ggcaatatcc tgctggattt cccgggtgat
    16681 atccggagcc ccctgtacca cggcaggcga caccagaatg accgcgtggt gtttctgctg
    16741 ccacgcctgc aggctggctt ccagtgccgt gttaaaacgc gctgaaaggg cttttgactg
    16801 tgcttctgac agttgtttct gactggcgct gtcaaaaaat gcatccacag tctgtttcat
    16861 gttgaaggcg gcagtcaccg gtgcgttcag tctgacaatg ccgtaactga cggctgcgtt
    16921 cagtaacacc attgccaggc atcccggcac cgtccaccac cagtggctgc gtcggggggc
    16981 tgtaacatca gtcgtttttt gcgttgtggt catgcaacac tcctgtattt ctcatgttct
    17041 tccagccagg cttccagcga agccatctcc ggccctgact tcttccacgc cagctgccac
    17101 accgcctcat gaatactcag gccctccctg cgtttctgct gcacaaactc aaaatccggg
    17161 ccgtcagagc tgtacatggc gcggcttaac gggtccacaa acagacggtg ccaggaggaa
    17221 tggttttcca cctgcagcag gaaggaactg aaccactggt ctttggccgc accaaacttg
    17281 ccaatcatgt cgcgctgcag tggctggaac tgatccggga ataactggtt atatttcgcg
    17341 aactccttcg cactctgttt gaggataatt ttgtaggagg agttacccca tgccgcacgg
    17401 gccgcactgg aggccttgtc agagtcaaag tcgacgatgt tctgtgtgat ggtgatatag
    17461 gcaccggtat ggcggcgaca ggtgcggtaa cctttttgaa taaactcacc gaccttgcga
    17521 tttttgaagt ccagcagacg ccagccttca tcaatgacgt tcagtttctt gagagtacgc
    17581 ggcgtgcggt acatcctgtt ctcgatgtag ataatcaggg agaacatcac cgcaaccagc
    17641 agtgacggac ggtcttccag tccgcccagc tccagcacca ccattttcgc atcatcccgc
    17701 agggacggct catcggagtt aaaatactgg ccgtaagtgc cgttggcagt gtactggtca
    17761 agcagcacaa tcatttcgtc cagacggctg cggatggtcg gtgacccggc atactgctcg
    17821 ctgtcactgg cgtttttcag gaaatccacc acgtcatcaa tacgtgccct gttctctttg
    17881 gccagccagg aggccctgac cgcctgcagc agcagacctt catgcacttc atccaggtta
    17941 ccgttggggc tggccatcac tgacaactgg tcacggacac gctccgctga ctggtcaata
    18001 tcggtgatgt tcgcaaacgg gttaaaacgc agggtttcac cgtccagata tacgcccccc
    18061 atgttctcac acagggactt gtagccatcc cccatgtcga acaccacggc aaagcccccg
    18121 gagtccagca cgctgcggat aagtggctgt atcagtccgg ttttaccggc cccggaggtg
    18181 ccacagaccg ccatgttgta gttggtgtta ttcatcccct taaagaagat atcgataaac
    18241 gccagctggt tacggtaggt gggcgccagc agacctgccg gtgtcagggg gttatccgcc
    18301 actaacggca tcaggttggc cacattaaag ctctctgcgc gctgaaccac accggcctct
    18361 ttcagctgtt taaacagccc tttcccggcc ataaagggca ggcaggtcag gaaattgcgc
    18421 atgtggttaa agcgcggtga aatcagctca aaaccgtttt tacggaagct gttcaggatg
    18481 tcctgctcca cttccagtgc cgtttcatta ttgtccttgc agaaggctgt gatgttgagg
    18541 aagtaggaca cgacagaaga ctggccggag cccagccgct ggcgcagttc cccccactcc
    18601 ttcgcctctt tctccacgga cggaaaccat ttggcatatg aggtcttcga cttcttctcc
    18661 aggtccatgt acttcaggtt ggcttcgctg tgggttttca cctggtcttc caccaccaga
    18721 gtcagcgtca ggatgaacgg gcaggaaatg gacatttccg ggttcagcag attgctgtag
    18781 ttgtcggcca tgttccacag gaaggcgatt tccgggttac gggccagatg gaaattcagg
    18841 atgcgggccg tgctgttcct gccgttctca cgcaggccca gcgtcaggta atcagcccgg
    18901 acattcaggt caaaactgtc ctccacacac tgataattca gatcagaata cgggtccagc
    18961 tgacgtcttt tcgggtacag agagtccgga ttatggttaa tcatctcccc gacaatctcg
    19021 atgaaagcct gtgcatccac tgtctgtgtg gtgatttttg cgccgtgaaa agaggcacgg
    19081 atgattttca ccagattttc catttccaga atgtcagccc ggcttttttt cttcgaggga
    19141 gaacagtacg agataaacac ccggtaatga cgcagggtca ggggcagatt catcccctcc
    19201 ggcagcggga actgtgtcgc tgccgctttc atataatagg cacgggtaat ggcgttaaac
    19261 cgttcagcct gttcacctga ccaggagaac tcacgcagcc cgtattcaat cctgtcacca
    19321 accagctgac tggacatcag atggatacac aacggaatac cgcgcggcag tttggtgcgc
    19381 agcatgtgat cgagtgcctc cacaatggac ttattcgccc cattgatggg aatggcttcc
    19441 agcataaagc ccatcgtggt gtcattcatg aacagaccgg attcctggtt gtaatcacgg
    19501 tacggcagca gacggctgaa ctgcgggaag ctcatttcgc ccagaacttc attggccttt
    19561 gcggattcgt caggcagttt cagtgctgtg acgagggagt taaccgcctg agtgacggcc
    19621 tcaagtgggt tattactcat atgagtattc cttagtgcgt ttaaaaaagg aaaatatcag
    19681 tctgtggccg tggatatatc acgcagtgcc attcctgaaa ccacagcaca aataagagat
    19741 ggaggctttt aacaggaaac caggattaac gttcctttac tgaatgagca tcatttaatg
    19801 cctgaatttg tttttcgcga atcttattgc gctcattttc tcttacccgt ttcacctctg
    19861 ccatttcgtt tctttttctg cattctgatg cgattttatt tgccttacca agtgttaaag
    19921 gttcagactg ataattaagt gtctcctgaa atgcttctgc tgcacaggga gtttcctgta
    19981 aaatctctgt cagttgttct ggtgttgcag gttgttgcgc aaggtaaacg atgtaaaaag
    20041 gaatacatac aatcatagcc ataacgccaa caaatataaa agccagaaca gattttaaat
    20101 atatcgactt catttcattt tccttcgaag cagtaagcac tcattgccca ccaccattta
    20161 gtgtttccat cacgctgttg aaaggttcat ttgttttaac ttcaatacac tcccctgtat
    20221 ttccaaagcc cctgccgcca tgtgcttcga ttgtccgaag aacctgcacg catacgtccg
    20281 tatttggcat gggtgtaata ttccagcttc cgccaccgtt aatatttttt atcagcacaa
    20341 gaaaaataaa ggtatgcatt ctttattatc tccgcttgta tattaatcac tgaatatccc
    20401 ttcagttaac ttatgaggtc ataaaacact gcagattttc attcagattt actctcttca
    20461 gcaaacttta ctctcttcag caaacttccg gatggtatcc tgttcacgct gacgggtggc
    20521 agactcctgc attgcatcca ctttctcctg ccagagacgc cggatatcct tttcatcccg
    20581 accagtcagt tttttaccgg acagcagacg gttaagcagt tcctgtctcg catccggtac
    20641 gtcttcagtc agacgcataa gctgaagcag gctctgacgg ctgagaacca tgagtgattc
    20701 cggcaggagg gtgaagaaga aaataaaaaa gcataccgca ccacagcagg caaggaccgc
    20761 aaacaggatt ttcagatata taccatcaac gccgaacgca gcaataatac agggaatcca
    20821 tgcaatacac gcacaatacg ccaggatgtt atctattcgg ttatgtctgc gatatatctg
    20881 ttgatgttca tccagaatag aatctatttt ttcagcttct gatttatgca tagtgtttcc
    20941 tctgcgtttc ctgccagaga cgccggatat cctgttcatc ccgaccagtc agttttttac
    21001 cggacaacaa acggttaagc agttcctgtc tcacatctgg tacgtcttca gtcagacgca
    21061 taagccggag taattcctga cgactgagta ccatgcgtga ttcctccggt acgctgtaat
    21121 aattaaaaat acgactgaat accccacaac agcaggcaac ggacaggcac agggatttca
    21181 gatacaggcc agaaacacag aatgtggcaa caccacaaag aatagctata actaaaaatc
    21241 ccttacccag tgccatacac aaagaaccct gttttctgga tatccgctca tgctcatcca
    21301 gaataaaatc tattttttca ttttctgatt tatgcatagt gtttcctctg cctttcctga
    21361 tacgcctgac attcaacgca caacgtcaca ccgggaaata ttttccgccg ggcttccgga
    21421 ataggatttc cgcatgcttc acagagataa acaggaatac catgaacatt tatttcctgg
    21481 cgtatccggt ttattcctgt catggtcagt tgttctgtca ctgaatatgc ttcatcggct
    21541 tcatcactca cggaatattc ctcccgacat gttctgacac gctaccgccc atcccttttc
    21601 acgcacaggg cactcgtttt acaggcggat tttgaataca gaaagcccgc cggcattgcc
    21661 ggctgatata caacttcagg gccagttaat taatacgtgg ttttccccac gcagacggtt
    21721 taataacaaa aaacacgctg gatggctgat gataaacatc ctggttatca atataaggcg
    21781 ctatccataa tgctgccgtc tgctcacccg ttcttagcgg acgaggaggt gttaccggcg
    21841 taactgaact gaccggaaca accgttttca cttcccgggc agcggtaaac agcgggcgtg
    21901 gtgccagcag tttctgctcc ggatgtgctg tcaccgcagg tctgctgccg gacggagtgg
    21961 tcgcggtgac ggtctgcacc ggcattgtcc ggaagttccc ttcagccagg cgcggcagtg
    22021 atgccgcaac cggctttgct tctgaggact gctccagttt tttggccttc tcattggcct
    22081 gctccatcgt catacaggta tcggatgtgg tggcgttaca ctcaaattcc gtatttgttc
    22141 ctgcgcagcc ggaaagtaat aaggttccca gcagaggaat aaataaagaa atctgtttca
    22201 ttgcccttct ccttttttat tactccccac aacagcagaa attaactctg ctgtcagatt
    22261 tgttttccct gcacagggat gtccggagac cactgtattt cccggagaag aaaataagct
    22321 catcgacatt cccttcttca tcacaccgac agcagccata ttaaatttgt gcggcaatga
    22381 ttcagaactg aacaccttcg gagcaggaag aactattttt gtggtgcagt tactgagaac
    22441 agcatgcata aaatcatctg atacgttttt atccggcaca acgcgggctg taataacatt
    22501 aatattacgc atgttcattt ttatctccgt tgcttctgtt tgttcggtca tcagggaaat
    22561 aaacatggca tttcctgatt tttcccggca gggaagcgtc ttccgtataa acgtccttca
    22621 gatattcatc tgcggttacc acccggatac atttcatttt tccgggcagc accggatatt
    22681 tcccggtgat cgctgccagc gagacataca tcagtgccag tgttgcccat aatgcggtga
    22741 ccagttgcat cagtgaccgg tcagtctccc tgacataatc tgcagcatca gccgcgcggt
    22801 aatggcagat atcctgtatt tctgatctgc ctgaagaatc agaggaagaa aaccagcatt
    22861 cactgaccgg gaactgtccg gcaggtgctg tttgtcttac gaagttaatc tccttaacca
    22921 gtaatttccc cggcgtggtg ttgtccttcc ataaaacaaa cagagccatc agggccattg
    22981 tggccagtgg cagaatgacg gtgtacttca gacaccagaa cagtcctcgc agaacccggg
    23041 cgacaacata ccgggcggca tggcacgcct gacgggacga catattattt gccatcgttg
    23101 cctcctccct ggctgccgga cataaactgc tgcggattca ggttgcccag ctgcttcagc
    23161 atctggtcag tattaaagcc gttaagatga ctttcagctg atggcggaac aggaaccgga
    23221 ctttccggat tatcttcttc cgctctgttc tgcgtgcgct ccagcgccat ctcttccacg
    23281 gttttcagct ggaagccgtc ctggaacacc acggtcactt cgttgcccgc accaatcggt
    23341 atcaccgggt gatactgttc ggcacgtttg atgtagtagt cactgagcgt ctgtgcggct
    23401 ttcgatgtgc cgccaccgat acccattttc aggacatcac cagccccgta agcggctgtg
    23461 gcacccagcc cgacagccgg ctgggaggcg cgctccattc cctgaccgat accgtcaaca
    23521 aatcccgcgc cccatgccca gccgaggatt ttgccgttac gcatcaccac ttcgcccttg
    23581 ataccgtttt taccccggaa gctgacatgc cccttaatcg gcatatcaat agttttgccg
    23641 tccttcaggc aactgatatt gcgggtgcgt acaatggcac gctcactgga cacatccccc
    23701 caggcttcca gccccacaaa acatcccgtt gcgtcatacg tcttgctgtt cggcatttcc
    23761 accaggccgg tgatacgcaa ctgcatcggc accgtggatt cattaccggt gactgaggca
    23821 ttggcatccg ccccttcaat cagcatggct ttcgcaaaac ttcctgacgg aatgtacggc
    23881 agcgatggtc cctgttttcc ctcattacgg gtaaacacct tacgctgtat ccggttaggc
    23941 accggcacag actggtacgt cacctgtggt ggtggcgtga caccattccc gggataaaac
    24001 gccgtcggcg gtggaacagc aacgctgccc tgcggcggga aggatacggg ggtgtttcct
    24061 ggctgtggtt cgccttccgg cccgggcggt gaggcaggca tctgaggtac aggctcaccg
    24121 gtcgccgtga cgggattagc acccagggct tttacctgct ctgccagggc ggcattgtcc
    24181 tgtcccagct tttcaatacg acgctggtca tcaccgcgct gtttgttcag gacatccagt
    24241 tcacgacgga tttcctcata ctgcttctgc atctgcgctg ccgtcacctg catctctgtg
    24301 gtggcatgct gacgcacctt gtcatcaaag gtcgtatcca ccacacctgt catatccggc
    24361 acaggctcct gttcagccac cgcctcaccg ttaccggaca tgtccacatc agacagatac
    24421 agtaccccac caatcgcgga ggctgcaccg acaaccacaa tccccagcca caggtactgc
    24481 ttgcgtttca caatggtatt gatactggcc atcagttgcc ctccccgtca cggatgacat
    24541 acacatccat gcgcgcacct gccagtaact ggctggcagg ctggctgaac atcacggcac
    24601 gggttcccgg ctgccagaag tcactttccc ggatattcag ggcagacagc gttttatttt
    24661 ccacggcaaa gcggaccatc ttcaggtgat tccccgtcca tacggcatct gccactgaag
    24721 acagccccgc cggggcctgc agggtttcct ttgtcacagg aacctgatac cagcctgcag
    24781 gtaattttcc gccacggaca gcctggctga tggtcacaag cagggattcg taaggcgtgg
    24841 acgtttccca cgctccggct tcttctccgg ttccgcgcag gtcactgacc agctgaatgg
    24901 tacgccccgc gccttcacgg ggaacagcct gaatggaaag gttcagacca cgctctgttt
    24961 ccagaatgaa cgtaaagggc tttttgttga cggtggccac caccacaccg ccggaggcgg
    25021 tctgctcatt attggtcagg gcaccatcca gactgttcac ggcgataatc cggtcaccgg
    25081 gaacggtaaa cagattcggg ctggtattgc tgatggcaat actggcctga ccaccgttca
    25141 ccattggcac cacagtgggg gccagcgtac cgtttgcggc catcacgctg caggaaaaaa
    25201 acaggccgcc gaatattatt gccgtattat tttttctcat cgtctgtttc cccgaaatta
    25261 tccagccagg tcaccccgtt ttcccgcttc agaataagga tgtaatgttt gatatcagta
    25321 aaaggttcgg agttaccaat ccatgttttc cgaattccac ggatttccac acgaccatac
    25381 tgaggccaga cacggacact ggtctggaaa aaagcagaat tcacgttatc ttttttaatg
    25441 agtttcgctt cttcagccag aataactttc atctggttct gtgcacccgg gcggatatat
    25501 tgcagaagcg cctgatgtga agcatcgacg atttccggtg agacattcag acggagggca
    25561 ataaatgaca gcgccatctg ctgcaaataa gaggcatcgg cactgttctg tgacacggca
    25621 aagggcacat taaatcccat tggggtcacg gcagtgcgtt gctcattctg aagacggtag
    25681 ttattcaccc cctgaatgag gttaacagag aggctgagaa caataagcac tgacataaat
    25741 ataaaggcga tggccattac acgactggta cttaaacggg caccgtgttc catataacaa
    25801 tcctggtatc agttctattt aatccactgc cggaaacacg aatcgggaac attatgaaaa
    25861 ataccgcgca gcagggctgt tggcatatac cagtaaatca ggtcacgtaa ccaggaactg
    25921 ccccgccctt ttttcagttt tttaatcccg aaataaacca gaaccgctgc accaataccg
    25981 aacagatatt tcgatgttgt gataccccag ccaacacaga ttgctgcggg gatcagttca
    26041 tccagtggca ggccaaacca gcggctctgg ttggtcagtg tttccgggaa acgatatttc
    26101 ttaagtttat tctcgtctcc cgacatcgtt ttatttcctg tcagaggcca acgacgctca
    26161 tacccacagc aataaataca gagatgatgg caaagccgac caggaacttg acgtttttgg
    26221 tcatcatgta catgaccgca ccgaccagca cctcagccag aacaacccat ttaacgatac
    26281 tggagtcctt accgaaggtc gccttaaccg tatcgttacc tttagccatc aggtcgcctt
    26341 taccagcagc catagctaac tcagggaaca acatcatcag aacagcaacc gggatcactg
    26401 cacgaaccag gcgaagcata ttcagacgag tgaatttaga aaaaaacgat ttattttcaa
    26461 cgggcgcaga agcaccctga acacttaaaa cagtattcat atgattcctc tgattaatat
    26521 tgacagatga aaactaaagt tctttaaatg ttgactcgtt ctcttcgatc acctccctga
    26581 atatttcttc attataaaaa tctggataac gttgcaaatg atcctgcaac ctcaccaatg
    26641 cttcgtttgt tttcgttctt ccactacgct ctctggctcc aagtaataat ttattcgttt
    26701 cctcatcaag ggtaagtaaa acttttctac ttggcgcaga tcttaaagct cctttaatca
    26761 ccctccctcc cataaataaa agagatttag tcaaaatatt cacataagac gcacacaaaa
    26821 ataaacaaca aaatagtaaa ttacgttaac aaaaaccggc acttttagtg ccgggaatgc
    26881 ttacatttct ttacagtgaa ggctgttaat attactagcg aagtattgat atagataaaa
    26941 taacacatca gagattatag catatcgcaa ccactcatcc ctgtctgaga aaccatagtg
    27001 ttttttaaaa ttcataaata tttttttaga ttggtcaaca gagatatcca ttctctgaga
    27061 agaatactca tgactataac ccaaaagata cggtattatc ttattaaagc gatatttgtc
    27121 aggttcacaa ctaaactcct tgccctttat aaaaaagcca ctaactacgt atctttcaaa
    27181 gaatctccag acaaacaaat tcaagttatt cacttccgta ctttcaacca ccacatccca
    27241 tgatacatca ttcaatatta caccattaat aatactcact ccattcgagg acgagaaagc
    27301 atcaacttca gcagtatgaa ataaaatttg cgtatcaata tcaataccag aaaaccatat
    27361 ttcagaatca ctattctgac actttattaa gtgtaaaaaa acagagtttg agtctatgaa
    27421 ttttcctgac tctgtgcgga cacagactgg atattttgcg gataaaataa tttccggcag
    27481 gcctgattga cgtatgagtg ctctttctct agtatccatc gcacacatag gaacctcctc
    27541 aaaggattct atggacagtc gatgcaggga gggacaagct ccctgcatcg gcgattttca
    27601 agttcaaatt ttaacgtggc attaaccacg taattaatat actaattatt ctgattattg
    27661 tcatcagttt gaaccattaa aaagtctgat atttgaacaa agtcaaaatt tatttccctt
    27721 tattcatcat cattttttgg aaagaacctt tccatctcag ccgacacctt ctctctgata
    27781 tcttcaacca tattggcata ttcaaacttt ggatttcctg agacatgagg actgagagac
    27841 tcaataccca aaattttcgc tactgatgac tgtgttttta caacgcattc aagaagtagt
    27901 ttattaaact cggcctgatt aaatgcagac tctttgcgct ccatctgagc ctcatataca
    27961 cgaagaccca gttcaagaag cattgaagcc gttgctgaaa aactgacatc tttttctctc
    28021 gccccctcct gtcgacgttt ttcaataatc gcgtttattt tttcataagc atcattgctg
    28081 atatacagat tcaccttagc cattagatat tacctttttt aaacataaaa caaagttaat
    28141 ttcgaaacac tagaattcat agtgcaaaca gggacgcacc gctagcagcg cccctgacgg
    28201 tatcctataa aaaaacacac cgcgccgcta gcagcacccc taatataaaa taatgttttt
    28261 tataaaaata gtcagtacca cccctacaaa gcggtgtcgg cgcgttgctg tagctgcgtt
    28321 aacgacgctg ctttaaaaaa atcagattta aacaatataa atccacaaat acaactcaat
    28381 gatattaaag ataaatcagc aaaaacttgt ttttgcgtag tgtgtggtgc ttttggtggt
    28441 gagaaccacc aacctgttga gcctttttgt ggagtgggtt aaattattta cggataaagt
    28501 caccagaggt ggaaaaatga aaaaatggat gttagcaatc tgcctgatgt ttataaatgg
    28561 gatctgcgaa gccgccgatt gctttgatct tgcaggtcgg gattacaaaa tagacccgga
    28621 tttactgaga gcgatatcat ggaaagaatc ccgttaccgg gttaatgcca tcggtattaa
    28681 tccggtaacg ggatatggca gcggactgat gcaggtagat tcccagcatt ttaacgaact
    28741 ggcccgctat ggaattaagc cggaacatct gacaacagat ccctgtatga acatttatac
    28801 cggtgcttat tatctggcaa tagcctttaa aaaatggggc gtctcctggg aggccgttgg
    28861 tgcatacaat gccggattca ggaagaccga acgccagaac cagagacgtc ttgcctacgc
    28921 atcagaggtt taccggattt atacctggat aaagagcagt aaaggcatcc gggttccgac
    28981 cacgaagaaa tcactttccc aaattaacag tgtgcagaaa aattaatatt gtatacattg
    29041 tcaaataccg gttatgccgc gacagcggca tggaaggcca cacacaaaaa tatcatatga
    29101 gtgataatgc tgactggccg aagccccgca aagcgggatc ccatcttata tatatccggt
    29161 aactgcgcag cctcctgcca gaccattttt cgcgggcaca atatttacag actaccgaaa
    29221 agccagttga tgaaattgag gcatatcaga cgacacagca atgccgcagg atgataatat
    29281 aagtaaagcc ccgaaaaatt tttcggggct ttacttatat tatcatgact tccactccct
    29341 gaactgttcg gcaatcatcc ataatgcttt gttgattcgg atatcaccgt caatgcctgt
    29401 aatggctctt gttctggtat ttttaccgga ggcacttcgc cccgataatc caccttttat
    29461 catattctcc tgaacccgct gccaggttgt ccagagatcg ttctgtttat cctcccaacg
    29521 gcggggagta attatttgtt ctggcgtcac tggcgtttta ttttcatctt cgtacctggc
    29581 catcaatgca gctctgccaa ataaatgttg ttcgtcactg ttaagatgta tctctttcat
    29641 tgtctccata ttttcagtga ctttatcaaa gacaccgagc acttcatacg ctccctcaat
    29701 aacctgcccg acaatatcac ctttatgtgg aacgcggatt tcgccaaaat tattcccaca
    29761 caccaggcca tttgtgcaga caaaacgaaa aattcctggg atcatctgat aactggatga
    29821 accatcatgt gaattaagca ggataatttc aggaacttct ttcccgttaa tatgcccttc
    29881 cctgcgaaga cgtaacatat gtttactgta ttcgcggcgt cccaaatcac gaacccgact
    29941 ctgacaggca aagaatggct ggaaaccttc atcacgtaac ttattgatga tattgatggt
    30001 tgggatatat gtataacgct cactccggga ttcatgttta tcaccagaaa agacagatgg
    30061 aacaaactgc attaattcgt catccgttaa cgggcgctca cggcgaatag aattatatcg
    30121 accaaaacgg gaagctaatc gcataacagt aactccttta tgaaggcata aaattaataa
    30181 aagttttttc gcttaatatc atcccccctc ggttatcgtt aacaaaaaga caacaattcc
    30241 gaggggaagg accatcaaat caggtcagtt cgggactgac caacctgccg agtgaaaagc
    30301 agtaacagcg cgatcaactc atcagcgttt tttctgacaa gaggagtatg attctccagg
    30361 ctatccagta agtcatgcag ctcattattg acctggtgga gcaccttaaa gagtgcacca
    30421 gaatcccggc agctctgata tgcaagattt gcctgaacag cacgattctg catgatcccg
    30481 atttgtgcga ttgcgtcttt ctgctgatcg cctgttggct gataagtggt gttaaaagaa
    30541 aatgcgctca taattcctcc tgtatctgat gttttcagcc cccatcatct gttgagtttc
    30601 gggagccgcg gttttgacgt tttgcgggga cctgcctttt gttccctccg gtattcacca
    30661 tcacctgttt gagaaccttc gccgcaaggg gcatcactgc cacggcctga cgcacctgtc
    30721 ctgggcagaa cgcaaaattt tttaccgggt tttgggtaaa aaattttgtg ggctgttcat
    30781 ttaccctgga cagtcagtgc ggcaggttgt ggctgtctat gcccccggcg acaggggctt
    30841 aaacaggtga atggcaccgg acagaacaac gggcagggac ccgccgcaac gacgtgaagc
    30901 gcggttcgcg aaacggcgtt gctccttaca tcccggcgtc ttttgccggg atgccgggag
    30961 cgcaggccac ggcgcgacaa gggtggcgcg accgttccgg tcgctgcaaa cccgggtttt
    31021 tgcgtcaggg atggaagccc gccagggcgg agacagcttc gctggctccg ttcacgacag
    31081 cccgccccgc cagggggacg cccggaaaag gcataagcag gagaacaaca aaacagacaa
    31141 taatcagggc agcagtaatc agagtcagag cagtaatcag agtcagagca ggaggaaaaa
    31201 gagaataatc aaagggaagg tgatgtgatt tctggtaagt cataaacaga gaaggcgggg
    31261 gaaaagaacg ggcgccgcag gcgcacgttc atccccgttc ccggagggaa acggccttcc
    31321 tggcagtctg gttgttcatg cccgtacaat gccagcaagg agaaaggggc caccggcgaa
    31381 ccagcagccc ctttataaag gcgcttcagt gcagtcagac cagcatcagc cctgaaaagg
    31441 cgggcctgcg cccgcctcca ggttgctact taccggattc gtaagccatg aaagccgcca
    31501 cctccctgtg tccgtctctg taacgaatct cgcacagcga ttttcgtgtc agataagtga
    31561 atatcaacag tgtgagacac acgatcaaca cacaccagac aagggaactt cgtggtagtt
    31621 tcatggcctt cttctccttg cgcaaagcgc ggtaagaggc tatcctgatg tggactagac
    31681 atagggatgc ctcgtggtgg ttaatgaaaa ttaacttact acggggctat cttctttctg
    31741 ccacacaaca cggtaacaaa ccaccttcac gtcatgaggc agaaagcctc aagcgccggg
    31801 cacatcatag cccatatccc tgcacgctga ccacactcac ttttcctgaa aataacccgc
    31861 tcattcaggc tgtttacggg taatctgtgt gattgttgcc gcatcacgcc gcctcccgga
    31921 gtttgttcgg caccatccac cgctccatct cacggacagc gtgaccggcc tctcttgctg
    31981 caagactggc ttccgcaata acctgccaga acggcatatc ctgccactgc cagcgttcca
    32041 ttccggccag tgacggaaaa cgctcacccc actgccagaa cagggtttcc aggtcatgct
    32101 ctgccacccg gtcactccag cgtgcatacc agcttccgac tgtttccggc gaatggaatg
    32161 ccagttcgat ttcagcctga gcgaccagcg cctggtacgc acgggctttc tgctctgcct
    32221 ccctcgcctg ctggcggctg tacttctgca tgaccagcgc ggatttatgg cgactgcgtt
    32281 ccctgacacg aaaccgcacc gacgggaaca agcgttctgc tgcatcgcct ggaagaggaa
    32341 ggaggcagac ctcgccctgt gtttcaatca gcacatcgat ggcatacagc cactgaagac
    32401 ggctgccgtg cagttcttct gcggtcaggg acggcatgaa aaatcgcagg tcagacagcg
    32461 atatcctgcg gcttccgttc agacagcgga aaaacgcccc cgcatacgga tattccgcca
    32521 gcgtgtttcc ctggtggcgg cgtttttctg tctccgccac cgcccgccat gcggcctgtt
    32581 gctctgcgct gaggggaacc agtgcctgtg aacgtgcgct catgcctgac ctccttccgc
    32641 catcaggata tggatgatgt cactgacgct gtacccctgc gcgtccagtg acgccgccag
    32701 tgccagtgaa tggctgacgg cttccggtgc aaaccgccgc tgtacctgaa ccaccgcaaa
    32761 cggctcacca ccggcattca ccagaaccag cacccagacc tgagagacat cgcccggtga
    32821 acataacgcc aggtgaaaac gttcgtgggc aggcgtgaaa cggacctgga cggggaaaaa
    32881 tccgccaaac tcgctgccgt actcgccgtt catcgtccag ttgtccggcg catccagttc
    32941 ccgcatcacg gcgtgtgtca gctcacgacg ttcatcactt ccggcagccc ggatatcttc
    33001 atattcctgt gcgctcatgg tctgtaatac attcagggtc agttcagttt tcatcacggt
    33061 atctcccttg ctgccgcccc gtctccggcg gggcggtgtg gttgttcatc aggcggcatc
    33121 agccaggtta tcggtgcgtt cagtggtatc tgtctgtgga tgtggtgcac acatccagcc
    33181 aggcacccag cggttgtctt tcatatggtg ctctgcatgt tcagccgcat cacctttctt
    33241 catcttctcc gcgtcccgtg cggcaccgga cagccctgcc tcattcaggg ctgcgataat
    33301 ctgcggcttt ttcaggtgtc cgaagaagtt tgcttttgtc ggctgccacc agtcgcgcat
    33361 gtgaaagccg atggcgcttt ccagcgtgtc aagcggactg cgtgacgtgt gaccacactc
    33421 acgggtctgg acaccgttaa ggctgcatgc ggtgcagaaa ctgagcaggg ataacagcac
    33481 ctcctgactg agtgacagga atgtcgtcat gtcccgtgac catccctccg gtaacagggc
    33541 agcaagacgg gctttttctg ccatcagcgc catgaatgcg gcaccttcct tccccgatgg
    33601 cgcatcgctg gtcagcgaat aatgtttaca ttccaggctg atttgtgctg gtttactgta
    33661 cgctccgctg ccaaacacat tcaggcagag cgtccatgcc agcagtgtca gggatttgtc
    33721 cggttgctgc atcagtgctg cctggactgc cagcgtgcgt tcggaagaca ttttcgtcag
    33781 cagtggcaga ctgatttcct ccactgacgc tttctcctgc acctgttccg tacggtcagc
    33841 gtcgtcagcc acgtcatcct cactgcgcaa ctgcacacca cgctggacac acacgttgcc
    33901 ataacgccag gacaccacca ctccacctcc ggcacgcatc tccggcgtcc acgccctgac
    33961 cttcgccatg cagcgcatca gcttcatttc tgcttccagc aggtcagatt cctcacactg
    34021 gttttccagc gcgtcgtaac gcgtcatcag ttcgttcaga cgttcttctt ccgcctccgt
    34081 cagcaccgct tccggctccg gcagacagcg gtatgttccg gcatcctcac ggcactcacc
    34141 gacaggctcc atgcgtccgg cgcaccattc ccagccttcg gcttcccgaa ggaactctgc
    34201 gacagcctgg agtttttcca gcagggcggc atcgagcgcc acgcagtcca catagccacc
    34261 ttcgtcgtcg ctgaacaaat cggtgcgcag ttcgtctggc gagaaggcat cagcccccac
    34321 gaagcggaat ttactgttcc ccgccaccgc cacttcactt tcggtcacca gacgacgaat
    34381 ggtctgtact tccggtttac cgccccatcc cgactgacag gcggcttcaa acacctgcac
    34441 ctgacgcgcg gtgttgttct ccagcgccag cgcctgacag tgttcggtgg tgatgcggtc
    34501 ttctgccagc gcatcgagga tgacaggcgc aaggtcagcc agtttcagca ttcgctgaac
    34561 gtggcggggt gaatagccca gcaaatcacc gatttgtgca ggtgttttgc cttcctgcgc
    34621 cattgcgcgg aatccggcaa tctgttcggc agggtgcata tcccgacgct gaccgttttc
    34681 agtcattgat gcagccgtcg ccagctcctg cggaataacc ttcacgcgta caggccagtc
    34741 agccggaagg atgccacgct ctgccagcat gttgagtgct gccagtcggc gaccacctgc
    34801 ggcgacaccg tggcggtcac caggcagggc atgaacaacc agattctgca acaggccaac
    34861 gcccttaatg gactccgcca gttcgctgac ggactctgcg gaatacggca ccgtgcgcac
    34921 attcagcggt gatttaatca gcgaggccag cgacacgctc acttcctccg tctgctccag
    34981 cagggcagac aggactgttt cctgcgtttt tgcaggttta cggctggatt tacgctccgt
    35041 ttttgtctta gactctgtaa ctgacatata tttctccttt gaccgggatg gttgtatgtc
    35101 tgccggacgc gttcccgtcc ggcacctctc ttacttaaga atgccgaaat tccggatgac
    35161 atggttcatc cagtacccgg caccgtcctc aaaattccag gtacgccaga ccaggccact
    35221 gttattgcgg acaaccaggc gaaaatgcgt tccctgatca tcctcgataa agacattccg
    35281 gtactgtgcg gcaacggctt cagcctgttc gcgggtaaag gagccgtcag gcagcccctg
    35341 aaatattctg aaatattcgc tcatcctcat atctccggtg atgccccgca ctggacgggg
    35401 cggttgtcac tgtctttcgt tagaacggaa tgtcgtcatc gaagtcatac gcgggttcag
    35461 acggctgctg aggctccggc tgcgcggcct tacgtccacg gccttttgtt ttcgcgctcc
    35521 cttttttcgt cgtgtcagca ctctgtggct gaccattctg ctgaggctgc ggttgcgcct
    35581 gagcgttctg ctgtggtgca ctccccagca tctgcatggt gcccgtggtt ttcacaagga
    35641 tttcagtgac gtaacgggta atacctttat cttcccagct acgggttcgg agttgcccct
    35701 cgatgtagac ctgcgcgccc ttgcgcagat attcacctgc cacttccgcg agcttgccga
    35761 acagcaccac gcgatgccat tctgtctgct cccgcatctc ccccgtctgt ttgtcacgcc
    35821 agctttctga cgtggccacc tgcaggtttg ccactgcgcc cccgttgggg atgtaacgga
    35881 cttccggatc ttttcccaga cgaccgacaa gaatgacctt gttaatgcca cgaactgcca
    35941 tgatgtgtct ccttctgttg atgttttcag cccccatcat ctgttgagtt tcgggagccg
    36001 cggttttgac gttttgcggg gacctgctct ttgttccctc cggtattcac catcacctgt
    36061 ttgagaacct tcgccgcatg gggcatcact gccacggcct gacgcacctg tcctgggcag
    36121 aacgcaaaat tttttaccgg gttttgggta aaaaattttg tgggctgttc atttaccctg
    36181 gacaggtgcg gcaggttgtg gctgtctatg cccccggcga caggggctta aacaggtgaa
    36241 tggcaccgga cagaacaacg ggcagggacc cgccgcaacg acgtgaagcg cggtttgcga
    36301 aacggcgttg ctccttacat cccggcgtct tttgccggga tgccgggagc gcaggccacg
    36361 gcgcgacaag ggtggcgcga ccgttccggt cgctgcaaac ccgggttttt gcgtcaggga
    36421 tggaagcccg ccagggcgga gacagctccg ctggctccgt tcacgacagc ccgccccgcc
    36481 agggggacgc ccggaaaagg cataagcagg agaacaacaa aacagacagt aatcagagca
    36541 gcagaaaaaa gaagaacagt agcagtagca gtagcagtag caggaacaac aacatcatca
    36601 gtaacaggca aatgacaggt ctgaaaagac agaagcccac agcagaacgt ccgcgtaatg
    36661 aaaggggcgg cgcgtaagcg cggccccttt cagcgccacc ggcgcgcggt cttttcccgg
    36721 cgcgggcaga cgggcattta tcctcccgcc tgcccggatt catcaggcac gcagacgaat
    36781 aatcatcgtc ggaacacggg tataagcaaa tgtgccgcac ggcagcacct cgcggacatc
    36841 agaaaacggt aacagcttct cctgctggcg cggcccgttc agacagacgg caaccagaac
    36901 gccccccgga cgcaacaggg aaaaagcccg caggatatgc cggatatcct gcccgtgact
    36961 gaacggcgga ttcataatga tccggctgta atactgcacc ggctgccatt ccatgaagtc
    37021 gccacactgt accctgacac cgttaaagtt ttcccgcaga taccggacca gcccgctgtt
    37081 aatctccacc gcatcacaca tggcctccgg cgcggtatcc cgaatcgccc gcagtatggc
    37141 cccggtgccg gcagagggtt ccagaatgtg atcacggttg ctgatttccg ccagcgtcac
    37201 caggcgatca cagaccgggg caggagtcac aaaaagctga gattcagccc ggatttcagc
    37261 aaccggcgcg gcgttatcca gccttgcgcg catcagccgg atacgttctc ttaactgact
    37321 gtgcatgtca ttatctccgt catcccccgg ggatttcccg gggggatata ccttatttct
    37381 gcgggatttc cgtgattttc atgtcggtga tatacacatt caccagacgg aaattattgt
    37441 ccattgtgcg gcggtagcgg tatgccccgt ggtcttctgc ttcttccaca ctgcgcacgg
    37501 ccttacagtc ccggggcagg gcggcccact gcgcctttgt catttcccgg aaaccttccc
    37561 ccggatagtt gactaccggc ggacgcttcg cggcctgact ggcgacggca gcctcttctg
    37621 ccgatggtgc tttgtagtcc gtgatgcgat cgggcgtcac tttcatcgtg ccgctgtacc
    37681 cgagaaaact gtaatttggc gttgtgactg aactcaccgc cccgttgctt ttgttcacgc
    37741 ggatgatagt cagccactcg ccccggctga aaacctgtcc gcccggctca aaatcctgtg
    37801 tccgggtaac cacgccgccg ctttcgtcca gcatggcacg ctcatagatc aggcggttct
    37861 gatagtggtt aacccagcgt tgctgatgct gaatctgccg ttcatgacag cggatcgcaa
    37921 tttcgcgggc ctgctccgtg gtgatgatgt catcatccag ggccgaccat aacgaccggc
    37981 tcccttcata ctggcttttt tctgccgggc gcggatacgt atccagcggg aaacaggcgc
    38041 tgatatggtc atgactgctg atgagttttg ccatattcag atccagcgat tcagcccgcc
    38101 acatcgtcag atatttctgc gactgtgcga tggtcttttc agccttgcgc aaatccgctt
    38161 cgattttttt gatacgacgc cagcgaacgt ccgggcgttc tttatacttc gcgtgaagca
    38221 gtgccgaccg cgcccgctcc tcccagtatt ccgcacgttc aaagagcatc acggcacgtt
    38281 tcatgccgtt ttcaatacgc tgcgcatcac gacgggcgcg gcgttcgctg tgatgcccca
    38341 caagaatggg ctgaccgggc gggatcatcg cggccagtct ttccacttca tcaagtgcct
    38401 gtgcggattc actggcccgc tttccgctgt atccggtaaa ccgctccgcc cgtgcttcct
    38461 gacgttcagc gagcgtgctg tcttcatcct caatctctcc ggcaagtgag aggagcacgt
    38521 cttcccggcc cggtgtccag gccggcgcga caaacagcgc ctgtttgggt gcccagcgaa
    38581 aaccggcatc atgcactttt ttatacgtct ccgggtcaag acgtgacgcg gcatacaggc
    38641 gcagtttgtt gtcatccggt gagtaggtcg cgcggtacat ctgcacaggg ctggcggctt
    38701 ccggggcggt catggtgatt gctgctaatg tcataaaatg ttctccttcc gtctgttgcc
    38761 gcccctcgcg gggcggctca cctcatcagt taaaagcgcg tttcagttcg agtgtgcgga
    38821 gcacctgccc gaaggccgtg tttttcatgg cggggatgtg tacaccacgc ccccggtgaa
    38881 aactccgggc tatgtgtcgc ccgaaggctt tcagcacagg gtcaatttcc ggcgcgggga
    38941 tgtcttcgcg tctgaaacgg ggatgacggc ggggctttac agccgctgcg gaaggtgaaa
    39001 caatacttat tgtcataatt tagcctctta tgacgattga ctggagccgc aaaaactgtt
    39061 cgtgcagtct ctgcggcatg gattaagcag gtccctgacc tgcttttttc atatctgcat
    39121 ctgacgtttt ccggtgtttt cctccttctg ttcctgcgtt ctcattacct gccagccgga
    39181 tggctgacag aacaaataaa tgcgtattgc cggagtctga aataaatcct gatttttaaa
    39241 gaacatcacc cgttgagttt cgggagccgc ggttttgacg tttcgcggga acctgctgct
    39301 gtattccctg tggtatgaac ttcacccgtt tgagaacctt cgccgcaagg ggcatcactg
    39361 ccacggcctg acgcacctgt cctgggcaga acgcaaaatt ttttaccggg ttttaggtaa
    39421 aaaattttgt gggctgttca tttaccctgg acagtcagtg cggcaggttg tggctgtcta
    39481 tgcccccggc gacagggact catacgggtg tgtggcaccc ggcaggcaat gagcagggac
    39541 ccgctgcaac gacgtgaagc gcggtttgcg aaacggcgtt gctccttaca tcccggcgtc
    39601 tttttgccgg gatgccagga gcgcaggcca cggcgcgaca agggcggcgc gaccgttccg
    39661 gtcgctgcaa gccgggggtt ttgcgtcagg gatggaagcc cgccagggcg gagacagctc
    39721 cgctggctcc gttcacgaca gcccgccccg ccagggggac gcccgggaaa gccataaaca
    39781 ggagaggaac ggaaagaaga agaggaagaa gaagaacagt aaccacagaa acataacatc
    39841 aggaaaaaac attccggaca catcacaaac gggaagggta acgggcgacg cgccagcgtg
    39901 gcccgcatcc cttccggcag gcgccagcct gccaggggtt ttcgcgcggg ctgacaggcg
    39961 gcctccaccg cctgcccctg tatcacctgt ttctgctgcc ctgtaagatg tagtgaccgc
    40021 ccaggaaaac cgcctgctga ttcgccacaa aatcgcagat ttcatcaaca atctgcgcgg
    40081 tggtgagcgg tttctgcccc atttcacgac gccgtgcatt aatcacggcc ctgtaattac
    40141 gcagaatttc gcgggtttcc gtgctgatat tcatgacatc gtctccccgt ccagcgcctg
    40201 caacacaacg tccattgctt cgagttcctc cctgacgaat gcgtcaccgt cgcccccgtt
    40261 ctcactcccg acggcataca ggcgcttcat ttcactgcct gccagcgcca tcactttttc
    40321 cagcatcacc gccgggactg tcacatgacg gctggcgcgc ttttcttcca tcagttcatt
    40381 gacaatatcg gaaacaactc tgtcacatgc gccatataca aaagcatcgt ccagccgctg
    40441 catgac
//
LOCUS       DAZXUL010000026        40005 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZXUL010000026 DAZXUL010000000
VERSION     DAZXUL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40005
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.026"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(24..278)
                     /locus_tag="ACH3L1_001307"
     CDS             complement(24..278)
                     /locus_tag="ACH3L1_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFT4076114.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="ACH3L1_001308"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="ACH3L1_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFT4076115.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="ACH3L1_001309"
     CDS             1228..2694
                     /locus_tag="ACH3L1_001309"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFT4076116.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="ACH3L1_001310"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="ACH3L1_001310"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFT4076117.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="ACH3L1_001311"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="ACH3L1_001311"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFT4076118.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="ACH3L1_001312"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="ACH3L1_001312"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFT4076119.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="ACH3L1_001313"
     CDS             5709..7265
                     /locus_tag="ACH3L1_001313"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFT4076120.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="ACH3L1_001314"
     CDS             7347..9161
                     /locus_tag="ACH3L1_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFT4076121.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="ACH3L1_001315"
     CDS             9162..10256
                     /locus_tag="ACH3L1_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFT4076122.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="ACH3L1_001316"
     CDS             10256..11281
                     /locus_tag="ACH3L1_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4076123.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="ACH3L1_001317"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="ACH3L1_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFT4076124.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="ACH3L1_001318"
     CDS             complement(12876..13220)
                     /locus_tag="ACH3L1_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFT4076125.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="ACH3L1_001319"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="ACH3L1_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFT4076126.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="ACH3L1_001320"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="ACH3L1_001320"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFT4076127.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="ACH3L1_001321"
     CDS             15616..17376
                     /locus_tag="ACH3L1_001321"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFT4076128.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="ACH3L1_001322"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="ACH3L1_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFT4076129.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="ACH3L1_001323"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="ACH3L1_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFT4076130.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="ACH3L1_001324"
     CDS             19113..19340
                     /locus_tag="ACH3L1_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFT4076131.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="yejM"
                     /locus_tag="ACH3L1_001325"
     CDS             19360..21120
                     /gene="yejM"
                     /locus_tag="ACH3L1_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFT4076132.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="ACH3L1_001326"
     tRNA            21195..21271
                     /locus_tag="ACH3L1_001326"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="ACH3L1_001327"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="ACH3L1_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFT4076133.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="ACH3L1_001328"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="ACH3L1_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFT4076134.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="ACH3L1_001329"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="ACH3L1_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFT4076135.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="ACH3L1_001330"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="ACH3L1_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFT4076136.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="ACH3L1_001331"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="ACH3L1_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFT4076137.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="ACH3L1_001332"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="ACH3L1_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFT4076138.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="ACH3L1_001333"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="ACH3L1_001333"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFT4076139.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="ACH3L1_001334"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="ACH3L1_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFT4076140.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="ACH3L1_001335"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="ACH3L1_001335"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFT4076141.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31429)
                     /gene="ccmA"
                     /locus_tag="ACH3L1_001336"
     CDS             complement(30806..31429)
                     /gene="ccmA"
                     /locus_tag="ACH3L1_001336"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFT4076142.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="ACH3L1_001337"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="ACH3L1_001337"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFT4076143.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="ACH3L1_001338"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="ACH3L1_001338"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFT4076144.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="ACH3L1_001339"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="ACH3L1_001339"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFT4076145.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="ACH3L1_001340"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="ACH3L1_001340"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFT4076146.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="ACH3L1_001341"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="ACH3L1_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFT4076147.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="ACH3L1_001342"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="ACH3L1_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFT4076148.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="ACH3L1_001343"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="ACH3L1_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFT4076149.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /gene="yojO"
                     /locus_tag="ACH3L1_001344"
     CDS             37391..37555
                     /gene="yojO"
                     /locus_tag="ACH3L1_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFT4076150.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37669..38178
                     /gene="eco"
                     /locus_tag="ACH3L1_001345"
     CDS             37669..38178
                     /gene="eco"
                     /locus_tag="ACH3L1_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFT4076151.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(38300..39924)
                     /gene="mqo"
                     /locus_tag="ACH3L1_001346"
                     /pseudo
     CDS             complement(38300..39924)
                     /gene="mqo"
                     /locus_tag="ACH3L1_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
BASE COUNT         9756 a        10813 c         9876 g         9560 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cggcgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc atgatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatt
//
LOCUS       DAZXUL010000027        39990 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZXUL010000027 DAZXUL010000000
VERSION     DAZXUL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39990
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.027"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..158
                     /locus_tag="ACH3L1_001347"
     CDS             <1..158
                     /locus_tag="ACH3L1_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFT4076152.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            387..977
                     /gene="uidR"
                     /locus_tag="ACH3L1_001348"
     CDS             387..977
                     /gene="uidR"
                     /locus_tag="ACH3L1_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFT4076153.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1365..3068
                     /gene="uidA"
                     /locus_tag="ACH3L1_001349"
     CDS             1365..3068
                     /gene="uidA"
                     /locus_tag="ACH3L1_001349"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFT4076154.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3074..4099
                     /gene="uidB"
                     /locus_tag="ACH3L1_001350"
                     /pseudo
     CDS             3074..4099
                     /gene="uidB"
                     /locus_tag="ACH3L1_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4138..5403
                     /gene="uidC"
                     /locus_tag="ACH3L1_001351"
     CDS             4138..5403
                     /gene="uidC"
                     /locus_tag="ACH3L1_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFT4076155.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5629..7137)
                     /locus_tag="ACH3L1_001352"
     CDS             complement(5629..7137)
                     /locus_tag="ACH3L1_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFT4076156.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7238..8413)
                     /gene="manA"
                     /locus_tag="ACH3L1_001353"
     CDS             complement(7238..8413)
                     /gene="manA"
                     /locus_tag="ACH3L1_001353"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFT4076157.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8612..10258
                     /gene="fumB"
                     /locus_tag="ACH3L1_001354"
     CDS             8612..10258
                     /gene="fumB"
                     /locus_tag="ACH3L1_001354"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFT4076158.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10401..11804
                     /gene="fumC"
                     /locus_tag="ACH3L1_001355"
     CDS             10401..11804
                     /gene="fumC"
                     /locus_tag="ACH3L1_001355"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFT4076159.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11801..12730)
                     /gene="tus"
                     /locus_tag="ACH3L1_001356"
     CDS             complement(11801..12730)
                     /gene="tus"
                     /locus_tag="ACH3L1_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFT4076160.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12806..14107)
                     /gene="rstB"
                     /locus_tag="ACH3L1_001357"
     CDS             complement(12806..14107)
                     /gene="rstB"
                     /locus_tag="ACH3L1_001357"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFT4076161.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14286..15605)
                     /locus_tag="ACH3L1_001358"
     CDS             complement(14286..15605)
                     /locus_tag="ACH3L1_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFT4076162.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15830..16573)
                     /gene="rstA"
                     /locus_tag="ACH3L1_001359"
     CDS             complement(15830..16573)
                     /gene="rstA"
                     /locus_tag="ACH3L1_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFT4076163.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16702..17037
                     /locus_tag="ACH3L1_001360"
     CDS             16702..17037
                     /locus_tag="ACH3L1_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFT4076164.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17034..17756)
                     /gene="folM"
                     /locus_tag="ACH3L1_001361"
     CDS             complement(17034..17756)
                     /gene="folM"
                     /locus_tag="ACH3L1_001361"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFT4076165.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17793..19175)
                     /locus_tag="ACH3L1_001362"
     CDS             complement(17793..19175)
                     /locus_tag="ACH3L1_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFT4076166.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19361..20305)
                     /gene="ydgH"
                     /locus_tag="ACH3L1_001363"
     CDS             complement(19361..20305)
                     /gene="ydgH"
                     /locus_tag="ACH3L1_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFT4076167.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20829..22361
                     /gene="pntA"
                     /locus_tag="ACH3L1_001364"
     CDS             20829..22361
                     /gene="pntA"
                     /locus_tag="ACH3L1_001364"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFT4076168.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22372..23760
                     /gene="pntB"
                     /locus_tag="ACH3L1_001365"
     CDS             22372..23760
                     /gene="pntB"
                     /locus_tag="ACH3L1_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFT4076169.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23785..24819)
                     /gene="tqsA"
                     /locus_tag="ACH3L1_001366"
     CDS             complement(23785..24819)
                     /gene="tqsA"
                     /locus_tag="ACH3L1_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFT4076170.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25230..25595
                     /gene="mdtJ"
                     /locus_tag="ACH3L1_001367"
     CDS             25230..25595
                     /gene="mdtJ"
                     /locus_tag="ACH3L1_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFT4076171.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25582..25911
                     /gene="mdtI"
                     /locus_tag="ACH3L1_001368"
     CDS             25582..25911
                     /gene="mdtI"
                     /locus_tag="ACH3L1_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFT4076172.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25950..26771)
                     /locus_tag="ACH3L1_001369"
     CDS             complement(25950..26771)
                     /locus_tag="ACH3L1_001369"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFT4076173.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26871..26954)
                     /locus_tag="ACH3L1_001370"
     CDS             complement(26871..26954)
                     /locus_tag="ACH3L1_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076174.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27047..27355)
                     /gene="asr"
                     /locus_tag="ACH3L1_001371"
     CDS             complement(27047..27355)
                     /gene="asr"
                     /locus_tag="ACH3L1_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFT4076175.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            27626..27730
                     /locus_tag="ACH3L1_001372"
     CDS             27626..27730
                     /locus_tag="ACH3L1_001372"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFT4076176.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(27779..29032)
                     /locus_tag="ACH3L1_001373"
                     /pseudo
     CDS             complement(27779..29032)
                     /locus_tag="ACH3L1_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29139..30050
                     /locus_tag="ACH3L1_001374"
     CDS             29139..30050
                     /locus_tag="ACH3L1_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFT4076177.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30185..31405
                     /gene="mlc"
                     /locus_tag="ACH3L1_001375"
                     /gene_synonym="dgsA"
     CDS             30185..31405
                     /gene="mlc"
                     /locus_tag="ACH3L1_001375"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFT4076178.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRRARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31530..32225
                     /gene="bioD"
                     /locus_tag="ACH3L1_001376"
     CDS             31530..32225
                     /gene="bioD"
                     /locus_tag="ACH3L1_001376"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFT4076179.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32178..33470)
                     /gene="clcB"
                     /locus_tag="ACH3L1_001377"
     CDS             complement(32178..33470)
                     /gene="clcB"
                     /locus_tag="ACH3L1_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFT4076180.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33629..34243)
                     /gene="dmsD"
                     /locus_tag="ACH3L1_001378"
     CDS             complement(33629..34243)
                     /gene="dmsD"
                     /locus_tag="ACH3L1_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFT4076181.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34286..35140)
                     /gene="ynfH"
                     /locus_tag="ACH3L1_001379"
     CDS             complement(34286..35140)
                     /gene="ynfH"
                     /locus_tag="ACH3L1_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFT4076182.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35142..35759)
                     /gene="ynfG"
                     /locus_tag="ACH3L1_001380"
     CDS             complement(35142..35759)
                     /gene="ynfG"
                     /locus_tag="ACH3L1_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFT4076183.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35770..37808)
                     /locus_tag="ACH3L1_001381"
                     /pseudo
     CDS             complement(35770..37808)
                     /locus_tag="ACH3L1_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(37869..38903)
                     /locus_tag="ACH3L1_001382"
                     /pseudo
     CDS             complement(37869..38903)
                     /locus_tag="ACH3L1_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            38961..>39990
                     /locus_tag="ACH3L1_001383"
     CDS             38961..>39990
                     /locus_tag="ACH3L1_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFT4076184.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
BASE COUNT        10168 a         9699 c        10240 g         9883 t
ORIGIN      
        1 aaattgaaca aaaaatgtta cagcacacgc cgatcagacg tctgggccaa ccgcaagata
       61 ttgctaacgc ggcgctgttc ctttgctcgc ctgcagccag ctgggtaagc ggacaaattc
      121 tcaccgtctc cggtggtggg gtacaggagc tcaattaata cactaacgaa ccggtaaacg
      181 gtcgtatgtg ttgtttaccg ggatagcgtg tgcaattcat caacgtctct gctaaacaac
      241 tggcagccaa atcacggcta ttggttaacc aattgctggg tgaaaagtat acgaatagag
      301 tgtaccttcg cactattcaa cagcaatgat aggcgctcac ctgacaacgc ggtaaactag
      361 ttattcacgc taactataat ggtttaatga tggataacat gcagactgaa gcacaaccga
      421 cacggacccg gatcctcaat gctgccagag agattttttc agaaaatgga tttcacagtg
      481 cctcgatgaa agccatctgt aaatcttgcg ccattagtcc tgggacgctc tatcaccatt
      541 tcatctccaa agaagccttg attcaggcga ttatcttaca ggaccaggag agggcgctgg
      601 ctcgtttccg ggaaccgatt gaagggattc attttgttga ctatatggtc gagtctattg
      661 tctctctcac ccatgaagcc tttgggcaac gggcgctggt ggtcgaaatt atggcggaag
      721 ggatgcgtaa cccacaggtc gccgccatgc ttaaaaataa gcatatgacg atcactgaat
      781 ttgttgccca gcggatgcgt gatgcccagc aaaaaggcga gataagccca gacatcaaca
      841 cggcaatgac ttcacgttta ctgctggatc tgacctacgg tgtactggcc gatatcgaag
      901 cggaagacct ggcgcgtgaa gcgtcgtttg ctcagggatt acgcgcgatg attggcggta
      961 tattaaccgc atcctgatcc tctcatttcg cgggctggtg ataactgtgc ccgcgtttca
     1021 tatcgtaatt tctctgtgca aaaattatcc tccccgtgtt cggagaattc ccccccaaaa
     1081 tattcaccgt agccatatgt catgagagtt tatcgttccc aacactctcg aacgaacgtt
     1141 cgattgctta ttttatggct tctgtcaacg ctgttttaaa gattaatgcg atccatatca
     1201 cgctgtgggt attgcagttt ttggtttttt gatcgcggtg tcagttcttt ttatttccat
     1261 ttctcttcca tgggtttctc acagataact gtgtgcaaca cagaattggt taactaatca
     1321 gattaaaggt tgaccagtat ttttatctta atgaggagtc ccttatgtta cgtcctgtag
     1381 aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg
     1441 aaaactgtgg aattgatcag cgttggtgcg aaagcgcgtt acaagaaagc cgggcaattg
     1501 cagtgccagg cagttttaac gatcagttcg ccgatgcaga tattcgtaat tatgtgggca
     1561 acgtctggta tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc
     1621 tgcgtttcga tgcggtcact cattacggca aagtgtgggt caataatcag gaagtgatgg
     1681 agcatcaggg cggctatacg ccatttgaag ccgatgtcac gccgtatgtt attgccggga
     1741 aaagtgtacg tatcaccgtt tgtgtgaaca acgaactgaa ctggcagact atcccgccgg
     1801 gaatggtgat taccgacgaa aacggcaaga aaaagcagtc ttacttccat gatttcttta
     1861 actatgccgg gatccatcgc agcgtaatgc tctacaccac gccgaacacc tgggtggacg
     1921 atatcaccgt ggtgacgcat gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg
     1981 tggtggccaa tggtgatatc agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa
     2041 ctggacaagg caccagcggg actttgcaag tggtgaatcc gcacctctgg caaccgggtg
     2101 aaggttatct ctatgaactg tgcgtcacag ccaaaagcca gacagagtgt gatatctacc
     2161 cgctgcgcgt cggcatccgg tcagtggcag tgaagggcga acagttcctg attaaccaca
     2221 aaccgttcta ctttactggc tttggtcgtc atgaagatgc ggatttgcga ggcaaaggat
     2281 tcgataacgt gctgatggtg cacgatcacg cattaatgga ctggattggg gccaactcct
     2341 accgtacctc gcattaccct tacgctgaag agatgctcga ctgggcagat gaacatggca
     2401 tcgtggtgat tgatgaaact gcagctgtcg gctttaacct ctctttaggc attggtttcg
     2461 aagcgggcaa caagccgaaa gaactgtaca gcgaagaggc tgtcaacggg gaaactcagc
     2521 aggcgcactt acaggcgatt aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg
     2581 tgatgtggag tattgccaac gaaccggata cccgtccgca aggtgcacgg gaatatttcg
     2641 cgccactggc ggaagcaacg cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg
     2701 taatgttctg cgacgctcac accgatacca tcagcgatct ctttgatgtg ctgtgcctga
     2761 accgttatta cggttggtat gtccaaagcg gcgatttgga aacggcagag aaggtactgg
     2821 aaaaagaact gctggcctgg caggagaaac tgcatcagcc gattatcatc accgaatacg
     2881 gcgtggatac gttagccggc ctgcactcaa tgtacaccga catgtggagt gaagagtatc
     2941 agtgtgcatg gctggatatg tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg
     3001 aacaggtatg gaatttcgcc gattttgcga cctcgcaagg catattgcgc gttggcggta
     3061 acaattgacc tacatgggcc tcgggctttg ctacagcctg gtgaatattc cttatggttc
     3121 acttgctacc gcgatgaccc aacaaccaca atctcgcgcc cgtctgggcg cggctcgtgg
     3181 gattgccgct tcattgacct ttgtatgcct ggcatttctg ataggaccga gcattaagaa
     3241 ctccagcccg gaagagatgg tgtcggtata ccatttctgg acgattgtgc tggtgattgc
     3301 cggaatggtg ctttacttca tctgcttcaa atcgacgcgt gaaaatgtgg tacgtatcgt
     3361 ggcgcagccg tcattgaaga tcagtttgca aaccctgaaa cggaatcgcc cgctgtttat
     3421 gttgtgcatc ggtgcgctgt gtgtgctgat ttcgaccttc gcggtcagcg cctcgtcgtt
     3481 gttctacgtg cgctatgtgt taaatgatac cgggctgttc actgtgctgg tactggtgca
     3541 aaacctggtt ggtactgtgg catcggcacc gcttgtgccg gggatggtcg cgaggatcgg
     3601 taaaaagaat accttcctga ttggcgcttt gctgggaacc tgcggttatc tgctattctt
     3661 ctgggtttcc gtctggtcac tgccggtggc gttggttgcg ttggccatcg cttcaattgg
     3721 tcagggcgtt actatgactg tgatgtgggc actggaagct gataccgtag aatacggtga
     3781 atacctgacc ggcgtgcgaa ttgaagggct cacctattca ctattctcat ttacccgtaa
     3841 atgcggtcag gcaatcggag gttcaattcc tgcctttatt ttgggattaa gcggatatat
     3901 cgccaatcag gtgtaaacgc cggaagttat tatgggcatc cgcacatcaa ttgccttagt
     3961 accttgcgga tttatgctac tggcattcgt tattatctgg ttttatccgc tcacggataa
     4021 aaaattcaaa gaaatcgtgg ttgaaattga taatcgtaaa aaagtgcagc agcaattaat
     4081 cagcgatatc actaattaat attcaataaa aataatcaga acatcaaagg tgcaactatg
     4141 agaaaaatag tggccatggc cgttatttgc ctgacggctg cctctggcct tacctctgct
     4201 tacgcggcgc aactggctga cgatgaagcg ggactacgca tcagactgaa aaacgaattg
     4261 cgcagggctg ataagcccag tgctggcgcg ggaagagata tttacgcatg ggtacaggga
     4321 ggattgctcg atttcaatag tggttattat tccaatattg ttggcgttga aggcggagcg
     4381 tattatgttt ataaattagg tgctcgtgct gatatgagta cccggtggta tcttgatggt
     4441 gataaaagtt ttggctttgc cctgggggca gtaaaaataa aacccagtga aaatagcctg
     4501 cttaaattag gtcgcttcgg gacggattat agttatggta gcttacctta tcgtattccg
     4561 ttaatggttg gcagttcgca acgtacatta ccgacagttt ctgaaggagc attaggttat
     4621 tgggctttaa cgccaaatat tgatctgtgg ggaatgtggc gttcacgagt atttttatgg
     4681 actgattcaa caaccggtat tcgtgatgaa ggggtgtata acagccagac gggaaaatac
     4741 gataaacatc gcgcacgttc ttttttagcc gccagttggt atgatgatac cagtcgctat
     4801 tctctggggg catcggtaca gaaagatgtt tccaatcaga tacaaagtat tctcgagaaa
     4861 agcataccgc tcgacccgaa ttatacgttg aaaggggagt tgctcggctt ttacgcgcag
     4921 ctcgaaggtt taagtcgtaa taccagccag cccaatgaaa cggcgttggt tagtggacaa
     4981 ttgacctgga atgcgccgtg gggaagcgta tttggcagtg gtggttattt gcgccatgca
     5041 atgaatggtg ccgtggtgga taccgacatt ggctatccct tttcattaag tcttgatcgt
     5101 aaccgtgaag gaatgcagtc ctggcaatta ggtgccaact accgtgtaac tccgcaattt
     5161 acgctgacat ttgcaccgat tgtgactcgc ggctatgaat ccagtaaacg agatgtgcgg
     5221 attgaaggcg cgggtatctt aggtggtatg aactatcggg tcagcgaagg gccgttacaa
     5281 gggatgaatt tctttcttgc tgccgataaa gggcgggaaa agcgcgatgg cagtacgctg
     5341 ggcgatcgcc tgaattactg ggatgtgaaa atgagtattc agtatgactt tatgctgaag
     5401 taagaaataa cgccggagag aaaaacctcc ggcgtttcag attgttgaca aagtgcgcgt
     5461 tttttatgcc ggatgcggcg taaacgcctt atccagccta caaaaactca taaattcaaa
     5521 gtgttgcagg aaaaggtagg cctgataagc gtagcgcatc aggcaatctc tggtttgttt
     5581 tcagatgaaa acgccggagt gaaaattctc cggcgttttg gccgtgaatt actgctgcgg
     5641 aattgccggt acagccggaa cgttaagagc tggcatcgca aacatgccaa caaaatcttc
     5701 taacgacatt ttctgcccat ttaacgttat ctgaccgtta gcatattgca ggctggtggt
     5761 gatggtattg tcctgcaagg tggtcagacg gaacatctgc cccattgctg atgcaccttc
     5821 aacttgctgt ttcgccagtt ttttcgcttg atcttcctga taaccttcca gcttcgctac
     5881 ctgagtcatc aactcagttg ccatatccac cggaatggtc agtttcgcat ccagagattt
     5941 aaccgaacga tctacttcct gcgccagcgt ttgcggcgct tctttagtcg ttgccggatc
     6001 tttcaggaac agcgacagat tcagcgcact ttcaccctga ctgtttttcc agcttagcgg
     6061 cgcgatagta atcaccggat cgcctttcaa catcagcggc agggcgctaa agaaggcttc
     6121 cgtcactttc tcctgataaa gttcgggatt gttggcaatt tctggctgtg ccagcagcgc
     6181 ctgagtttgc gcgttatatt gctggctaaa ctgatgccag gcttcaccat caatctggcc
     6241 gacttttaaa gtcagcttgc cgctgcccag atcctgattc tgtaccttca ggctgtttag
     6301 cgagtaatcc agttggctat tgatcgtttt accgtcattg accagatccg atttaccgct
     6361 gatctccatg ccttccagca gtgccagttc tttgccttcc actgaaatgg tcattttttc
     6421 cagtgacagt ttttggtttc ctacgcgctc accaaaactt cccagcgtgc tggaaccgtc
     6481 ggttttcaga ttattaaagg tcaactgcac tttctggttg tattcgttaa ctgcgtctat
     6541 ccgaccactt tgcgcctctc cggaaaggga gatggctttg ccgtctctgt cagcatttaa
     6601 ctggaactcg ccgccgctaa aggcgacttt ttcatccttt tgctcgtaat tcagtggatt
     6661 gagcgaaata tcggaactgg aatcaccgct gtaaccaatg cgcgagttaa tctcaaaagg
     6721 cgtttcacct tttgccatat caaacagtgg tttgcttact tcgttattaa ccagcgtggt
     6781 ttgaattgat gccatcgacg ggatcaggtt cagtttttta agctgggcaa gcgggaaggg
     6841 accatgatca accgattcgt tgaagatgac gctctgaccg cttttaatcc acggattttc
     6901 tttcccggca atgggtttca ccaacagttg caactggctg ctgaatacgc cgcgatgata
     6961 gttttgataa ctcacttcca ggttggattc aggagctgtc agtttgagtt gcgcgttcgc
     7021 ctgcgcgacc atgtcttcga gatgggtttc aatcttcttg cctgtatacc atgcgccgcc
     7081 tgtccagact acgcctagcg caacaatgac gcctaccgct accagcgatt tattcataat
     7141 gattatccat aaaatgaaat caggcggact ggccgcctga aggtgttata agcctttaat
     7201 aagcttagca agagatgtta attttttcag taagctctta cagcttgttg taaacacgcg
     7261 ctaaacggcc gtggcctttg acagtcaccg gtgattcgtt ggcggcaata aacgctgatt
     7321 cacccggttt aagctgtaac tgctgagaac ctttccacaa cgttgcatcg ccttcgacgc
     7381 agaacaaaat ggcggcactc tgctggctga tggtggtttc tttatcacta aggtcatgca
     7441 gcgagaaggc aaaatcatcc acaggaatcg ggaagtccag ttctgcacct tgtttcaccg
     7501 gctgggtcaa caactggtta gccggtttgg cttcgaattt cacattggca accagttccg
     7561 gaatatcaat gtatttaggc gtcagacctg cacgcagcac gttatcggag ttcgccatca
     7621 cttccagcgc cacgccttgc aggtaagcgt gcggtgtttc agcgaacagg aacatcgctt
     7681 cgccaggatt caatttcacc acattcagta acagcgggga gaacaggccg ctgtcttccg
     7741 ggtaaaattc agaaattaaa cgaatcgttt gccacggttc accctgctga ctgtcgagcg
     7801 ccgattttaa aatcgccagc gcgcgggatt tttcttcacc ctgcatattc aacaggttgg
     7861 cgaacagttc gcttaaacgt tcggcatgtg gctgttgtaa aaagtgagca atcgccggat
     7921 gtgcaccggc gaccggctgg agtagggaga caatctcgga aaattcacga aacgcgttca
     7981 tcgcaaggaa aggcgtcagc gcaaaaacca gctccggctt gtggttagga tctttatagt
     8041 tacgctcggc ggcatccatc gggatacctg cggcattttc tttggcaaaa ccgatttcag
     8101 aattgtgttt gtttggatga acctgaatgg agagtggctg tgctgcgcat aatactttga
     8161 acaggaaagg cagttcgcca aagcgtttgg caacggcctc tccgagcaga gtcgatttat
     8221 cactctcaat cacatcacgc agtgaaacga tatctccggc ggcattctgc actcgtgaac
     8281 tgcttttcgg atgtgcgccc atccacagct cggccatcgg ctggctggac ggattttcca
     8341 taccataaag ttcagtcaac gccgttttgc taccccaggc atagttttgc actgagttaa
     8401 tgagtttttg catgatcaat ccctgtttta atgtggaaat taatcccgct attaaagcaa
     8461 gaatcctacg ggaagtaacc tggagccgca aaaagtcgta ctagtctcag tttttgttaa
     8521 aaaactgtgt aggatattgt tactcgcttt taacagggca acggaacacc cgcccagagc
     8581 ataaccaaac caggcagtaa gtgagagaac aatgtcaaac aaaccctttc attatcagtc
     8641 tccttttcca ctcaaaaaaa atgatactga gtattacctg ctaaccagtg aacacgttag
     8701 cgtatctgaa tttgaagggc aggagatttt gaaagtcgca cccgaagcgt taactctgtt
     8761 ggcgcgtcag gcgtttcatg atgcgtcgtt tatgctgcgt ccggctcacc aacaacaggt
     8821 ggccgacatt ctgcgtgacc cggaggccag cgaaaatgat aaatatgtgg cgctgcaatt
     8881 cctgcgtaac tccgacatcg cggcgaaagg cgttctgcca acctgtcagg ataccggcac
     8941 cgcgattatt gttggtaaaa aagggcagcg tgtatggacc ggtggtggtg atgaagcggc
     9001 gctggcgcgc ggtgtctata acacttatat cgaagataat ctgcgctact cgcaaaacgc
     9061 gccgctggat atgtataaag aagtgaatac cggcaccaat ctgccagcgc agatcgatct
     9121 ttatgccgtt gatggcgacg aatacaaatt cctctgtatc gcaaaaggtg gtggttcggc
     9181 aaacaagacg tatctctatc aggaaaccaa agcgttactg acgccgggga aactgaaaaa
     9241 ttacctggtt gagaagatgc gcacgctggg taccgcggcc tgtcctccgt atcatattgc
     9301 gttcgttatt ggtggaactt ctgcagaaac gaaccttaaa acggtgaaac tggcttccgc
     9361 gaaatactat gatgaactgc caacggaagg gaatgagcac ggtcaggcgt tccgcgatgt
     9421 ggaactggaa aaagaattac tgatcgaagc gcaaaatctc ggtctgggtg cgcagtttgg
     9481 tggtaaatac ttcgctcacg acatccgcgt gattcgcctg ccacgtcacg gcgcatcctg
     9541 tccggttggt atgggtgttt cctgctctgc tgaccgtaat atcaaagcga agatcaaccg
     9601 tcaggggatc tggatcgaaa aactggaaca taatccaggt aaatatatcc ctgaagagct
     9661 gcgtaaagcg ggagaaggcg aagcggtgcg cgttgacctt aaccgtccga tgaaagagat
     9721 cctcgcacag ttgtcgcagt atcccgtttc tacacgctta tcgcttaacg gcacgattat
     9781 cgttggtcgt gatattgctc acgccaaact gaaagagcgg atggataacg gtgaagggct
     9841 gccgcaatac attaaagatc atccgattta ctacgcgggt ccggccaaaa cgccggaagg
     9901 ttatgcctcc ggttctcttg gcccaacgac cgccggacgg atggattctt atgtcgatca
     9961 actgcaagcg cagggcggaa gtatgatcat gctggcgaaa ggcaaccgca gccagcaggt
    10021 gacggatgcc tgtaaaaaac acggcggctt ctaccttggc agtatcggtg gtccggccgc
    10081 tgtattggcg cagggaagta ttaagagcct ggaatgtgtt gaatatccgg aactgggaat
    10141 ggaagccatc tggaaaattg aagtggaaga tttcccggcg tttatccttg tggatgataa
    10201 aggaaatgac ttcttccagc agatacaact cacacagtgc actcgctgtg tgaaataaac
    10261 agagccgccc ttcggggcgg tttttttaca tggcacgaaa gaccaaacat ttgttatcaa
    10321 atagtaaata atgagtgagc taaaagttgc ttaacgaaag caaaacagaa agaaaaaatt
    10381 aatcaggtga ggagcaggcc atgaatacag tacgcagcga aaaagattcg atgggggcga
    10441 ttgatgtccc ggcagataag ctgtggggcg cacaaactca acgctcgctg gagcatttcc
    10501 gcatttcgac ggagaaaatg cccacctcac tgattcatgc gctggcgcta accaagcgcg
    10561 cagcggcaaa agttaatgaa gatttagact tgttgtctga agagaaagcg agcgccattc
    10621 ggcaggcggc ggatgaagta ctggcaggac agcatgacga cgaattcccg ctggctatct
    10681 ggcagaccgg ctccggcacg caaagtaaca tgaacatgaa cgaagtgctg gctaaccggg
    10741 ccagtgaatt actcggcggt gtgcgcggga tggaacgtaa agttcaccct aacgacgacg
    10801 tgaacaaaag ccaaagttcc aacgatgtct ttccgacggc gatgcacgtt gcggcgctgc
    10861 tggcactgcg caagcaactc attccgcagc ttaaaaccct gacacagacg ctgagtgaaa
    10921 aatcgcgtgc atttgccgat atcgtcaaaa tcggtcgaac ccacttgcag gacgccacgc
    10981 cgctaacact agggcaggag atttccggct gggtagcgat gctcgagcat aatctcaaac
    11041 atatcgaata cagcctgcct cacgtagcgg aactggctct tggcggtaca gcggtgggta
    11101 ctggactaaa tacccatccg gagtatgcgc gtcgcgtagc agatgaactg gcagtcatta
    11161 cctgtgcacc gtttgttacc gcgccgaaca aatttgaagc gcttgcgacc tgtgatgccc
    11221 tggttcaggc gcacggcgcg ttgaaagggt tggctgcgtc actgatgaaa atcgccaatg
    11281 atgtccgctg gctggcctct ggcccgcgct gcggaattgg tgaaatctca atcccggaaa
    11341 atgagccggg cagctcaatc atgccgggga aagtgaaccc aacccagtgt gaagcattaa
    11401 ccatgctctg ctgtcaggta atggggaacg acgtggcgat caacatgggt ggcgcttccg
    11461 gtaactttga actgaacgtc ttccgtccga tggtgatcca taatttcctg caatcggtgc
    11521 gcttgctggc agatggcatg gaaagtttta acaaacactg cgcagtgggt attgaaccga
    11581 atcgtgagcg aatcaatcaa ttactcaatg aatcgctgat gctggtgact gcgcttaaca
    11641 cccacattgg ttatgacaaa gccgcggaga tcgccaaaaa agcgcataaa gaagggctga
    11701 ccttaaaagc tgcggccctt gcgctggggt atcttagcga agccgagttt gacagctggg
    11761 tacggccaga acagatggtc ggcagtatga aagccgggcg ttaatctgca acatacaggt
    11821 gcagccgtgg aatgatcaaa cgaagcggct gcgcctgagg tttataacgg tgctgcacat
    11881 tgtcggcatc gtaatttaac aactcaccaa cgtccggcac gcccgcgcca ttatcccgat
    11941 taatcagtgc aatcagtggt gtagggcagg cgtgttggac ttgtttttga tctcctttgt
    12001 accagacgcg ggcaatcggc tgcaccttca ctggacgttt gatttttaac ttcgcgttct
    12061 gtggcagggc agcgatatcc tgatactctc gctccagttt tctttgccac tcctcgcgcg
    12121 tccacggtgc gacactgcgt ggtgatttca ggcttttttc cagctgtgcc aggacttcat
    12181 cacgatgcaa attcttaatg atatgtttat tagcccaacc aaagcgtaaa gtggcggggt
    12241 cgtgcagaac ggtgagcgtg cggtaagcat taagggtgat cagccccggc aaatgacgat
    12301 gcacccattc aaaacgtgcc gcggtgggga gttctgattc aaccgtgacg atatgctcga
    12361 acgtggtctt gagtttattg atgtgctgaa tatgactgac caacgctgct tgcgaaaggt
    12421 tatcgacctg gtaacacaac acgccaggca gacggacagc ggccttgctg ctgcgatttt
    12481 cggactgttg ttgaataaat aaatggcgga aatgacgcaa cgccagcgac tgcgcgtcgt
    12541 tgccgagatg ttgttttacc tcaatacgat taagcggatt atgctcatcc tcttttttta
    12601 cctccggcaa agagaacacg cgggcaacca atagcttgtg ttgctcaaga tgagcggcaa
    12661 atgtagccag ctcttgttcc atctggcgaa aggtagtgtt gagtcggtct acgagatcgt
    12721 aacgcgccat aatattaacc actttagtta caacatactt attttatagc acagtcgtgg
    12781 tgacttgacc atcgtgcgcg tagtgtcagg cagaggtaaa ttgcgggatg ttatgccata
    12841 acggccagct aaacgagaag cgagcaccac ccagttcgct gatgtcacaa ttaaccgtac
    12901 cgcccattgt cagtgctatt gagtggacaa ttgccagccc cagcccgcag ccgccggttg
    12961 agcgatcccg gctgggatcg aggcgaacaa aaggttcaaa gatatgttcg cggttttctg
    13021 gggcaatccc tgggccatca tcctcaacaa ttaatgtcgc tctattcccc gacagtagca
    13081 ggctggtttc aaccgttgaa tggcagtagc gcagggcgtt attgagcaaa ttatccagca
    13141 cgcgctccat taagcgcata tccaacgccg cataatggcc ttgcatgagc gtttttatcc
    13201 gtaccgtttt atcgggcgtt actgcctgaa tatctgccag atgcgttgac agccacaacg
    13261 gcaggtctgg ttcgctaaga tgaagctcgt tttgtgggcg atcgagtcgg gcataagtca
    13321 gcagctcttc aattaaagct tcaagttgac tgatatcacg attcaacgcc tgggattcgg
    13381 cggcgctcag gttatcgctc atctccagtc gataacgcag gcgcactaac ggtgttcgca
    13441 gttcgtgagc gataccgtca ataagctgtt ttttgctggc aattaaggcg ttgatattgt
    13501 ccgccatctg gttaaatgcg ataccaagtc gttcaaagct cgaaccctca tcaaagtgga
    13561 tacgttcatt gagatgccca tcgccaaatc gttgcgccgc tgcttccagt tttaacatat
    13621 cctgccagtg cggacgcatc cagataaaca ccggaaaggc gagggaaata gcaataaaag
    13681 cgatcagggc gatatccagc aatcgcattt gatggaggta ataaagataa ggaacaggac
    13741 caactgccag cacgtagtgg ctgcgcggga tacgctgcaa aaacgtgtac tgatcgtcca
    13801 gggcgacaat ttcgccgcca cgcagtcggt gcatggaaat atcatcaaga tggtatttac
    13861 tcagtggctc gacacgcaga tcgaaagaga gatttaaatc catctctttc agcgtcttac
    13921 cccagtcgtg tggggggatc tctcgcaatt cgctgcgcat cagatacagc gaactgttca
    13981 tcaaatcatc cagcgactgt ttgcccgcgc gttcggcggt aaatttgtac accagcccaa
    14041 ccagcagaga catcacaagg aagcagacaa acaataacag gtaaaactgg ataaacaatt
    14101 ttttcattgg catcgcccgc aacagaatac tattcgtatt aattcacgac tgtggagcaa
    14161 tgtaaaccgc caactggata agaccttgtt tggaagataa tgtccggaac agacggatgt
    14221 atccccggtg tattcatatt cagtaaattc aattttccac cggggcttgt ttattatgct
    14281 aaggtttaat ttaacgtgac gctttgttgt tctttagtct ctctatcctt aagaagacgg
    14341 gctgatatta caccggacat tattgaacca ttcacattaa gcgctgttct tgccatatca
    14401 acgatcggct ctatggcaat aaaaataact actaatgcga cagggaaatt aagtgttgaa
    14461 aggacaatca gcgccgcgaa tgtcccacca ctacctacgc ctgctacccc tattgagcca
    14521 agcgcgatgg caggtaaaag agttgcgagg aattgcaatg agaagggatc gatacccatt
    14581 gttggagcaa tcatcgccac catcacggca ggataaatcc cagcgcagcc attttgtccc
    14641 atgctggaac caaaagacgc tgcaatatta gcaatcgtat ttggaacccc caatttttcc
    14701 aatgcagaaa tagccagagg aattgaagcc gcactactgc gagaaacaaa cgcaaaggta
    14761 agtacttgcc agacggtctt gtaatagtta acggggtttt tacctattaa cagcaaaatg
    14821 cccgagtgaa caataaacat cacaaaaacg gccagataac aggcgacgat gaacccgagc
    14881 aaattggtaa actgtgaaag ctgatacgtt gagaagactt tcgccattag tgccattacc
    14941 ccataaggcg tcagcgcaat gacaatacgg accattttca ttacccatat ttgaatcgcg
    15001 ttgatccccg cgctcaattt ttcgccctct gctggtgcat ctttctttac ttttaacagc
    15061 gcaataccag caagaattgt aaagatgacg atgccaataa ccgaaacact tcttgccccg
    15121 gtcagatcaa ggaagaggtt ggttgggatc aatgacgtaa tgagtcgcgg taaagatacc
    15181 gcagctgttt tcgagacgtc atcgctggtt aaagaggatt gcatatgacc aaaggcgctg
    15241 gcatcaaggc caattgcact ggcaatagcc aggccaacta aacctgcaat catgactaaa
    15301 cacaacatac aggcaacaat agttaaagac atcttgccaa taccagcgct attttcgagc
    15361 ttattaatcg ctgaaagaat cgaaatgaat atgagtggaa cggcaacaag ttttaataaa
    15421 ttaacataac cgttaccagc aatattatac cattcgataa cggtttcact gcttgtctca
    15481 ggaatgaaat gcagggagat accaatgata gcccccagga aaatcgcgat aaaaacactt
    15541 ttggaaaggg attcgctctt ttgatccgtt ttgaaaatcc cccatgagca gatcaaaaaa
    15601 agcacaatat atgtgaaagt tgtaaccgtt ttttaagcct cttgcctcat tcagcacctg
    15661 aaatgccgga aagagtttgt tgtaaaaaat ctgcgccata agacgcaggg acgaggcgat
    15721 tatagagaaa ttttttctca ttttcatatc aaatgtaggg ggtttggaat ttgaataagc
    15781 tcttattaat tgaattcgtt aattgttctc tttttcagat gaacaaatat tagtcatctg
    15841 attgtttatt gtcactatcc catgcatgag gcgcaaaaag atagcctttg ttacgcacag
    15901 ttttaatgcg ataaggttct gcggcgttat cgagcagttt ttttcttaac cgcgaaatag
    15961 ccacgtccac gctacgatcc agtccgtcat aactgacgcc gcgtaaattt ttcagcaatg
    16021 catcgcggtc catgatttgc ccggcatgcg tagctagttc ccacaataat tcgaaatcag
    16081 ctgtcgagag cgagatttca gtgttagcca gggtgactac gcggttgatg gggtcgatag
    16141 ttaacgtgcc gaaatgcagg gctttgtagg gagtaagaga cgtttcctga agacctttgg
    16201 tcagtgtggc ttgctcattc tgacgcaaat gcaaacgtaa acgcgctagc aaaacagcag
    16261 ggggcgtcgt tttgagaata tagtcgcagg cacccatttc cagtgccagg atgtggttca
    16321 tatcgctatc gagagaggtt agaagaacaa tcggtccaga ccactttgcg cgtaaatcac
    16381 gacaaatggt catgccgtcc ttgcctggta gcatgatgtc gagtaacacc aaatccggat
    16441 tttctcgcaa aatggtttct tcggcctggt cgccgcgcgg ttctacggta acctgcatat
    16501 catgttttgc caggtacgcg gcaatcagtg aaccgacttc cgcatcatct tccacaaata
    16561 cgatagtgtt cataacattc acggtagata taaaaacgtc acaatacacc gcgctgtttt
    16621 tacttactat caatcgtttc taaacaagtt ttcatcaggt attctactga tgacttgtta
    16681 tggaagtgtt aaggtaaaaa gatggggctg gtaatcaaag ccgcgctcgg tgcgctggta
    16741 gtgctgttga ttggtgtttt agcaaaaacg aaaaattatt atatcgccgg gctgattcca
    16801 cttttcccga cctttgcgct tatcgcgcat tatattgttg ccagcgaacg cggcattgaa
    16861 gccttacgcg caaccatcat ttttagtatg tggtcgatta ttccctattt tgtttacctg
    16921 gtgtcgctgt ggtatttcac cggaatgatg cgcttgcccg ccgcttttgt tggttctgtt
    16981 gcgtgttggg ggataagcgc atgggtgttg attatatgct ggataaagct gcattaacgc
    17041 agatgacgac cgccatcaag tgggaaactg cgtccggtga caaagcaact ggtaagtaag
    17101 taatcgacca ggtcgatcac ttctttctcg ccaggcgcgg ttttcatcag tgatttattc
    17161 agcgcctgtt gtcgatattc ggcatcatca tgttcattaa acaggatcag cgatggcgca
    17221 atagaattca ctttcacttc cggtgccagc ttgcgggcaa acgagcgggt catattatcc
    17281 agtgccgctt tgcttgcagc atacgcaaca tgtttgtcgc taccgcgctc caccacataa
    17341 tcggtaaagt gaatgatatc gctggcggcg tgtccgtgcc cacgcagtaa tctttccagc
    17401 gcatggttga gcaggtatgg ggtattaacg tggatctgca tcatgcaagc cagtacgtcg
    17461 gccagtggcg cacccggttt ttccgcaatc cacgcactgg cgttatgcaa aatagcacgc
    17521 agaccatggg tggtttttag tacttcatcg gcaaacgcca tcacaccgtc gttggtcgaa
    17581 aaatcagcct gaatacactg cgcacctgca ttaatcagtc catcaatggc tggatagtgt
    17641 gtccgatagc tgacaatcac cggttgcttt tgattaatga aatgccatgc gagggcgagg
    17701 ccgatgcgac gacctccgcc agtaattaat attggcaagg gctgggtttt acccatcgtt
    17761 atctcctttg ctatccaacg atgggatgaa cgttatccca ccagcatcca ggtcgccgga
    17821 actgaggcaa ttaacagcat gccaatcagc accatttcct gacggttcag tacattatca
    17881 tgtgtatgcg ttttgcgcgc atacaggaaa accagcagcc ccggtgcata cagaacaacg
    17941 gataacagca agtgcattgg accagaggcg tacaataacc ataagccata aatgcaggca
    18001 ccgacaccta ccgctttatg cagtggacgg gttgcgattt tcagcaagaa cgcgccgacc
    18061 aggaaatagg gcaccagaat catttctgag gcgatggtca gcaacgtgtt gtaatcggaa
    18121 ccggtcagcc agatgagtac cagacaaatt tgcacgcaga tgttggtcaa ccacagtgag
    18181 gcggatggcg cagcttgtgc gttctggcgc gcaaaaatgc gtgggaatgc tttatgagtg
    18241 gcagccagga acggaacttc tgccgccatg atggtccagc ttaagtacgc gccgcaaacg
    18301 gaaacgatca aaccggcagc gatgatgatt tcgccccatg gtcccatcat ttccaccatc
    18361 agaccggcca ttgacgggtt acgaatttca gccagttcag gacgtgccac cacacccagc
    18421 gaaagcagcg ttaccagcaa gtaaacgccc agagcggaga gaaccgccag cagtgtcgct
    18481 ttgccaacat cacgtttatt acgcgcacgc gcagaaacaa ccacggcacc ttccacacca
    18541 atgaaaaccc acagggtgat cagcatggtg tttttcacct gttcccaaac aggtacgcca
    18601 agtgcaagtc cggtgaagtc gagcttgaag gtatccagtt tgaacatcat catcgccagc
    18661 acaacaaaca gacccagcgg caacaatttt gccagtgtcg ccaccaggtt aatgctggca
    18721 gcggtttgca ccccgcgcag gatcaaaaag tgaacaatcc acaataaagc cgatgcaccg
    18781 actatcgatt gccaggtatt accatcgcca aacaggcgca attccggcgt gtccgtaaaa
    18841 aagcttaacg cggaaaaaac gatcaccaga taggagacgt tggcgatgac cgcgcacagc
    18901 cagtatcccc atgcggaaca aaagccgatt agctcgccaa acccttcgcg ggcataggta
    18961 aagataccgc catcaagttc gggacgaatg cgcgtgagga tcagcatggc aaaggccagc
    19021 aataaaatgc cagcgccagt aataccccag ccgatgagca gtgctgccgg gctggcaact
    19081 gccgccatat tttgcggcag actgaaaaca cccgcgccca gcattgagct taataccagc
    19141 gcggtgagtg cgctcagtcc cagtttcttt tccatcgata tccagtgatt aaaacgtgat
    19201 aagaagaata aatattaggg caaaacgcat aaaaatggag tttgccactg aagcgcggga
    19261 ttttacgaag agatgtgtct gcatgcaatg gtcgcgggga aatttgtgac taaaaatgcg
    19321 aaaggcggca tcaagccgcc ttataggagt aactgaatag ttatttatac agatctgcgc
    19381 tgacggtcag gttattacca cgtttctgcc actggcgggt gatgtggtaa tacttagcac
    19441 ctttcttcgc ggcacgtttc gcaacctgat aggagacttc ggtcatgttg ccgtagttgc
    19501 cagagaattt gatgctgtcg aacgggacca tcatcgctgc tgtcgctttg ttcagttctt
    19561 cgactttagt gccatccggg agcgtgacgg tgtaacgccc gccttttgat gactgggttt
    19621 caaagaagcg accgacttca gaacttggtg atgcggtagt cgcaacaccc gggatctcaa
    19681 ctttcttcgc ggcttcgcca ccggcagcca gagctgcacg tcctgcttcg gaatctgccg
    19741 ggatcacatc cgggctctgg acgatacgtt tcttagcatc ttttttatag atgaatgcag
    19801 taatacgctg gttgccgccc tggttggcat cgatttgacg aacgatgtag aaagagtagg
    19861 cacctttcgc ttttgccgct ttggtgatgg catcattgac ttctggctgg ctacgataga
    19921 agccctggac ggtgaccgtg tcaaacggtt caatcagaac agcctgatct ttcggcagtt
    19981 cgacaacacc gttaattacg cgattacttg tttcttctgc tttttcagca tcggctttat
    20041 agaggtcagc gaccacacgc cagttaccgc tgttaccaaa atcagaagtg tcgacaacat
    20101 aaaaagaggc ggcaccttct ttatctgcgc gacgagaaac ggctttcacc gcttcgccaa
    20161 tagcattaaa acgaccggta accactacac ggtcaaaagg tttaaccgct gccgcttgct
    20221 ccggtgtcag ttctgttgct gcgttaacgg agaatgccgt agcagaaagc agtgccgacg
    20281 ccaggagggt gttcttaagc ttcataaaaa taatccttcg ccttgcgcaa accaggtact
    20341 ggtattgtta ttaacgagaa acgtggctga ttattgcatt taaacggtgt aactgtctgc
    20401 gtcatttttc atatcacatt ccttaaacca attttaatcc tgctcaaatg accgtctatg
    20461 cttaaaaaac agccgtatca gcatcattac tactgaagca actgaattgt ataagttaat
    20521 ttaatgttaa gtagtgattc gtgccggggc gatgtctcgt tttacccgac cgtcgaagac
    20581 aattatcagt ctttatccgg cgttctaagg tgtttatccc actatcacgg ctgaatcgtt
    20641 aatattttgc gagttcacgc cgaaatactg atttttggcg ctagatcaca ggcataattt
    20701 tcagtacgtt atagggcgtt tgttactaat ttattttaac ggagtaacat ttagctcgta
    20761 catgagcagc ttgtgtggct cctgacacag gcaaaccatc atcaataaaa ccgatggaag
    20821 ggaatatcat gcgaattggc ataccaagag aacggttaac caatgaaacc cgtgttgcag
    20881 caacgccaaa aacagtggaa cagctgctga aactgggttt taccgtcgcg gtagagagcg
    20941 gcgcgggtca actggcaagt tttgacgata aagcgtttgt gcaagcgggc gctgaaattg
    21001 tagaagggaa tagcgtctgg cagtcagaga tcattctgaa ggtcaatgcg ccgttagatg
    21061 atgaaattgc gttactgaat cccgggacaa cgctggtgag ttttatctgg cctgcgcaga
    21121 atccggaatt aatgcaaaaa cttgcggaac gtaacgtgac cgtgatggcg atggactctg
    21181 tgccgcgtat ctcacgcgca caatcgctgg acgcactaag ctcgatggcg aatatcgccg
    21241 gttatcgcgc cattgttgaa gcggcacatg aatttgggcg cttctttacc gggcaaatca
    21301 ctgcggccgg gaaagtgcca ccggcaaaag tgatggtgat tggtgccggt gttgcaggtt
    21361 tggccgccat tggcgcagca aacagtctcg gcgcgattgt gcgtgcattc gacacccgcc
    21421 cggaagtgaa agaacaagtt caaagtatgg gcgcggaatt cctcgagctg gattttaaag
    21481 aggaagcggg cagcggcgat ggctatgcca aagtgatgtc ggacgcgttc atcaaagcgg
    21541 aaatggaact ctttgccgcc caggcaaaag aggtcgatat cattgtcacc accgcgctta
    21601 ttccaggcaa accagcgccg aagctaatta cccgtgaaat ggttgactcc atgaaggcgg
    21661 gcagtgtgat tgtcgacctg gcagcccaaa acggtggcaa ctgtgaatac atcgtgccgg
    21721 gtgaaatctt cagtacggaa aatggtgtca aagtgattgg ttataccgat cttccgggcc
    21781 gtctgccgac gcaatcctca cagctttacg gtactaacct cgttaatctg ctgaaactgt
    21841 tgtgcaaaga gaaagacggc aatatcactg ttgattttga tgatgtggtg attcgcggcg
    21901 tgaccgtgat ccgtgcgggc gaaattacct ggccggcacc gccgattcag gtatcagctc
    21961 agccgcaggc ggcacaaaaa gcggcaccgg aagtgaaaac tgaggaaaaa tgtgcctgct
    22021 caccgtggcg taaatacgcg ttgatggcgc tggcaatcat cctgttcggt tggctgtcaa
    22081 gcgttgcgcc gaaagagttt cttgggcact tcaccgtttt cgcgctggcc tgcgttgtcg
    22141 gttattacgt ggtgtggaat gtatcgcacg cgctgcatac accgttgatg tcggttacca
    22201 acgcgatttc agggattatt gttgtcggag cactgttgca gattggccag ggaggctggg
    22261 ttagcttcct tagttttatc gcggtgctta tagccagcat taatattttc ggtggcttca
    22321 ccgtgactca gcgcatgctg aaaatgttcc gcaaaaatta aggggtaaca tatgtctgga
    22381 ggattagtta cagctgcata cattgttgcc gcgatcctgt ttatcttcag tctggccggt
    22441 ctttcgaaac atgaaacgtc tcgccagggt aacaacttcg gtatcgccgg gatggcgatt
    22501 gcgttaatcg caaccatttt tggaccggat acgggtaatg ttggctggat cttgctggcg
    22561 atggtcattg gtggggcaat tggtatccgg ctggcgaaga aagttgaaat gaccgaaatg
    22621 ccagaactgg tggcgatcct gcacagcttc gtgggtctgg cggcagtgct ggttggcttt
    22681 aacagttatc tgcatcatga cgcgggaatg gcaccgattc tggtcaatat tcacctgacg
    22741 gaagtgttcc tcggtatctt catcggggcg gtaacgttca cgggctcggt ggtggcgttc
    22801 ggcaaactgt gtggcaagat ttcgtctaaa ccgttgatgc tgccaaaccg tcacaaaatg
    22861 aacctggcgg ctctggtcgt ttccttcttg ctgctgattg tatttgttcg cacggacagc
    22921 gtcggcctgc aagtgctggc attgctgata atgaccgcca ttgcgctggt attcggctgg
    22981 catttagtcg cctccatcgg tggtgcagat atgccggtgg tggtgtcgat gctgaactcg
    23041 tactccggct gggcggctgc ggctgcgggc tttatgctca gcaacgacct gctgattgtg
    23101 accggtgcgc tggtcggttc ttcgggggct atcctttctt acattatgtg taaggcgatg
    23161 aaccgttcct ttatcagcgt tattgcgggt ggtttcggca ccgacggctc ttctactggc
    23221 gatgatcagg aagtgggtga gcaccgcgaa atcaccgcag aagagacagc ggaactgctg
    23281 aaaaactccc attcagtgat cattactccg gggtacggca tggcagtcgc gcaggcgcaa
    23341 tatcctgtcg ctgaaattac cgagaaactc cgcgctcgtg gtattaatgt gcgtttcggt
    23401 atccacccgg ttgcggggcg tttgcctgga catatgaacg tattgctggc tgaagcaaaa
    23461 gtaccgtatg acatcgtgct ggaaatggac gagatcaacg atgactttgc tgataccgat
    23521 accgtactgg tgattggtgc taacgatacg gttaacccgg cggcgcagga tgatccgaag
    23581 agtccgattg ctggtatgcc tgtgctggaa gtgtggaaag cgcagaacgt gattgtcttt
    23641 aaacgttcga tgaacactgg ctatgctggt gtgcaaaacc cgctgttctt caaggaaaac
    23701 acccacatgc tgtttggtga cgccaaagcc agcgtggatg caatcctgaa agctctgtaa
    23761 ccctgacggc ctctgcggag gccgtcactc tttattgaga tcgcttaaca gaacggcgat
    23821 gctttgacct cccgctgttt gttcaagcgc aattttgaca ataattgtca acggcacgga
    23881 aagcagcata cccaccggtc ctaacaacca tccccaaaaa atcaacgaca aaaataccac
    23941 caatgtcgaa agccccagcc cacgtcccat gatgcgcggc tccagaatat tgccgaagac
    24001 cagattaatc agcagatatc ccgccagcac cagcaacgct tcgtagaagc cattaaacac
    24061 cagtacctga gcgatagggg ggattgccgc gagaactgaa ccaatattag ggatgtaatt
    24121 aagcgcaaag gccagcaatc cccagacaaa agcgaagcga acatcgagtg cggcgagcat
    24181 cgcccaggcg accaggccgg tgatgatgct gatggctgtt ttcagcacca gataatgaga
    24241 aacactgtca atcgcacgtt gaatcgccgc catcccttca accggacgcg ccatcatttg
    24301 ctgaaatttt ccgggcaatt gtggcacttc gagcagcata aacagcaccg tcagcagcaa
    24361 taaaaatatt gatgacatgg cattagataa ctgcgtcaat aagttggtga gcaacgtcat
    24421 cgccgcgttc ggatcaatgt aatgcgccag ttggtcaact gagacgtcaa tccctacgcg
    24481 ttgcaacaac ggctcaagag cttgcagcgg cgtcataata gagttgcgat attgcggtaa
    24541 cgtccgcgtc aactcgttga gcgcggaacc cagataagct aatagcaaca ccatcgccat
    24601 cacgatgatt gtcatcaaaa tcgacactgc cagtacacgc ggcacacgcc agcggaccat
    24661 atgttgcacc agcgggttaa gaataacagc aataaataat gcgagaataa acggcacgat
    24721 gatctcggcg gcaaaacgga taccgcagag aataatgacc agcattccca acataatgac
    24781 gatttttagg ccattgagcg tgatgatcgg ctttgccatg cttgtccccg tttttcttcc
    24841 tttgctaata ataatgtctt tttcgctccg tcatcataac gaaaatcaaa ccgctcaggt
    24901 aaagaagtga aaatattttg agttaattct taagctatga tacaaatcag gcgtgttcaa
    24961 ctaccgagga caattatcat ctgcgatgac gagaagcaac actgcggata attgtaatat
    25021 tatggacaat ttgttcagga cgttattttc tacatttacc catcttcgta cctcatccac
    25081 tatcttgctc gtaggtgagc agcactggcg caacgcgctt tagtcctttc ttctgcctaa
    25141 gcttgcccta tgcgcagggc ttgaccactt tactttggtt tcgctgaatt aagcgaaaat
    25201 taaaatagtt ctcttgcagg agaaggacaa tgtatattta ttggatttta ttaggtctgg
    25261 ctattgctac agaaattacc ggtacgctgt caatgaaatg ggcgagcgtc agtgagggaa
    25321 atggcggctt tattttaatg ctggtgatga tttctctgtc gtatatattt ctctctttcg
    25381 ccgttaaaaa aatcgcctta ggcgtagctt atgcgctgtg ggaaggtatc ggtattttat
    25441 ttattacctt gtttagcgtt ttgttattcg acgaaagttt atcgctgatg aaaattgccg
    25501 ggttaaccac cctggtcgcc gggatagtgt tgatcaaatc aggtacccgt aaagcgcgta
    25561 aacctgaact ggaggtgaac catggcgcag tttgaatggg ttcacgccgc ctggctggca
    25621 ttggcaatcg tgctggaaat cgttgctaac gtctttttga aattttctga cggctttcgt
    25681 cgcaaaatat ttggcttgct ctccctggcg gcggtgctgg cggcctttag tgcgctttct
    25741 caggccgtta aagggatcga cttgtctgtc gtttatgcat tgtggggcgg gtttggtatt
    25801 gccgccacgt tagccgcagg ttggatcttg tttggtcaac ggttaaatcg taaaggctgg
    25861 attggcctgg tcttgctgtt ggctggaatg atcatggtga aacttgcctg atgaagacgc
    25921 tgcccgcgct gtcgggcagc gtttgaacat tatttttgcg acagttgatc cagcttgtcg
    25981 cgaaaaccgg taacagaaat ggcccggtta tcggcgcgcc agcgatcttt cgcggcagga
    26041 gccgaacttt gcaccccaat taattgccag ccgtcatcgg tatgcaacat cagaggcgaa
    26101 ccgctgtcac ccggcaaggt atcgcactga tgtgacatca ccgacgtttg cgcccagcca
    26161 gtcacttcac agttttgatg actgtataac gtatcgagat gatcttcagg gtatcccgcc
    26221 tgagtcactt tacgaccagc cgattttaat gcggcagtaa gcgcggcttt atctccctca
    26281 aataacggca acggtgtaat gccagaaggg ggattacgta gcacaatcaa tccgaagtcc
    26341 cacggtgcag ctgcgggagg aacaatccaa ccatccccat ctgcttttaa ccgctttccc
    26401 agtgtcggat caacgcggcc ttctatgtcg tggatctcat agcgccaaag acctttattt
    26461 gacacaaaac gcagcgctac cgccttatcc gctttaccct taggcggcgt caataaacag
    26521 tgtcctgccg ttaatgccag attgggtgca atcagcgtcg ccgtacataa attgccgctg
    26581 gccgtttcca gttgcccaac cgcatcccac ggtgattggg tcgtgtcatt cactggcaca
    26641 cgatcatcat gaccaaaaaa cagggtgctg acctcatcgt ttgccgattt ggcaacgtct
    26701 ggtttatctg caaacacaaa agcagacgtc aaactaattg cacccaacac tacagcaatg
    26761 gttgtacgca tatcacactc tggtgggtaa ttatgattat taaaagcaaa ccctcataaa
    26821 tactatagac gggacgggat gaaagtggga gtaaaatcag ataactacag tcaggaaaga
    26881 taaaaacggg cggcaatcag tgcgcataaa ataaggatga taagaatgaa ctcaaagcga
    26941 taacggccca ccatactacc ctccgctaaa ggcggtgcta aacgttaccc gcttagcacc
    27001 gtaattttca ggcgcgaggg ggcgcgccag cattactgtt gaaaacttac gctgcgggtt
    27061 gtgcagcagg ttttgccggt tgctgatggc cgtgtttccc ggcgtgtttc ttcgctgcct
    27121 gcgctttttg ttcaggggct ttctgttcag ctttcgtatt tttatgatgc tttttagccg
    27181 cctgcgcttt ctgggcaggt gctgctttat gctgtttttt atgatgtgta gttttcgctg
    27241 gcgctgcttt ggtggtcgtc gcagtcggag caggtgtggt cgcagtctct gcagcaaagg
    27301 cggcagaaga cagacccata gcagcggcaa caaccagagc taatactttt ttcattgtca
    27361 taccctcaat ttgttttttc atttaacccc actgcggggc cgttgaaata actatatccc
    27421 tgggaatggc agacttccgt gagtggttgg tttcagcgtg taacgatatg tacaaacgct
    27481 gaataaatta cagcgttgat aataacgtat tgtgacttaa ggaaaattta gctaccaata
    27541 atagtagtct tgatcgggtt aactattata ctacttacaa agcggtagaa taactgcgca
    27601 tcagtaataa atgacacaca gcaaaatgaa tccgtttatt tgggtactta taatcctgat
    27661 gacgctagat gcgctgcggg aattggctgg cgcttcgtct attttaggat ggctattaac
    27721 gttggtttga gcaggcaata agtccggacg ggtatttacc gcagtccgga cttatttttc
    27781 aggcgtgcag acgacgatgc aaacgcgtcc cgaccagcag agcaatgacc agcatcagag
    27841 caataaatgc gccgacgccg ttccagccat agttatgcca gaaaacacca cccagcgtcc
    27901 cggcaatact cgaccccaga tagtaactga acagatacag cgaggaggcc tggcctttag
    27961 cgcgttttgc gcgggggccg atccagctgc tggctactga gtgggctgcg aagaatccgg
    28021 ctgagaagag taacattccg gcaaagatca gccacagcga gctaaataag gtcatcagta
    28081 aaccaaacgg cataactccc gtcgaaaaca acatcaccgg accgcgccca tagcgggtgg
    28141 tcatggttcc ggctttgggt gagctccatg taccggtcaa ataagccagc gataataagc
    28201 caaccacggc ctgactgaca tgctaggggg aaagcatcaa ccgatagccg atgtaattaa
    28261 acagcgtgac gaacgacccc atcagcaaaa agccttctgc gaacaataac ggtaatcccc
    28321 ggtcacgcca gtgcagacga aagttgataa ataacgtctt agggcgcagc gaagtcgggc
    28381 aaaaatggcg tgattcaggg aggattttcc agaacatcaa cgccgaggcc agcgcgaaac
    28441 aaccgattgc cgccagagca attcgccagt tgaaaaagtc cgtgaagaca ccactaatta
    28501 agcgtccgct catgccgcca attgagttgc cgctgatata caaccccatc gaaaaggcca
    28561 cgaaactggg gtggatttcc tcgctaagat aagtcatgcc aacagctgcc acgccactta
    28621 acgaaagccc aatcaaggcg cgcataatca aaatgccgtg ccagctggtc atcattgtcg
    28681 aaagtaacgt acaaatggag gccaacagta gcgccgtgac catcactggt ttgcgaccaa
    28741 tggcatcgga tagcgggcca gtaaatagca aaccgatagc caacatcgcc gtggaaatgg
    28801 acagtgaaat actactgttc gcgggcgtta agccaaactc ctgcgaaagc accggaagga
    28861 taggctgcac acaatagaga agtgcaaagg ttgccagtcc ggcagagaac agcgccaggg
    28921 tgacgcgcat aaattgcggc gtaccgcgtt taataaattg atttggctga gaaatgcttt
    28981 gcttgtcagt gtcgcttgcc ggagcgccat caacagttgt agtacggctc acttgaaatc
    29041 cttgctaaat atgcctgtgg atcaggctta tacatagggt aggaaaatcg aattgttctg
    29101 tctaatatat taataatctc aaataagatg ttttaaatat gaatattgaa cttcgtcatc
    29161 tgcgttactt tgttgctgtt gcggaagagc tgcattttgg gcgcgcggct gcccgcctga
    29221 atatttcgca accgccgcta agtcagcaga ttcaggcact ggagcaacaa atcggtgccc
    29281 gactgctggc acgaactaat cgcagtgtat tgctgacggc agcaggaaaa cagtttcttg
    29341 cagatagtcg gcaaatcctg tctatggtgg atgacgctgc cgctcgcgct gaaagactgc
    29401 atcagggtga agcgggggag ttgcgtattg gttttacttc gtccgctcct tttattcggg
    29461 cggtgtccga tacgttatcg ctgtttcgcc gtgattatcc tgatgtccat ttacaaaccc
    29521 gcgaaatgaa cactcgcgag caaatcgctc cgctcattga aggaacgctg gatatgggat
    29581 tgctgcgtaa cacagcgtta ccggagacgc ttgaacacgc agtcatcgtc catgaaccgc
    29641 ttatggcgat gatcccgtac gatcatcccc tggcaaataa tccgagtgta acactggctg
    29701 aactggctga actggctgaa ctggcgaaag aaccctttgt cttttttgat ccgcacgtcg
    29761 ggactgggct gtatgacgat attctcggac tgatgcgacg ttaccatttg acgcccgtca
    29821 tcactcagga ggtgggcgag gcaatgacca tcatcggtct ggtttccgcc ggtctgggtg
    29881 tttcaatttt gcctgcgtca tttaaacgtg ttcagctcaa cgaaatgcgc tgggtgccga
    29941 ttgctgaaga ggatgcggtt tctgaaatgt ggttggtctg gccgaaacat catgaacaaa
    30001 gtccagctgc gcgtaacttt cgtattcatc tgctgaatgc tctcaggtga gggaaatttc
    30061 agcgaaaaag cccgaaaaat gtgctgttaa tcacatgcct aagtaaaaat ttgacgacac
    30121 gtattgaagt gcttcaccat agcctacaga ttatttcgga gcgcgaaaat atagggagta
    30181 tgcggtggtt gctgaaaacc agcctgggca cattgatcaa ataaagcaga ccaacgcggg
    30241 cgcggtttat cgcctgattg atcagcttgg tccagtctcg cgtatcgatc tttcccgtct
    30301 ggcgcagctg gctcctgcca gtatcactaa aattgtccgt gagatgctcg aagcacacct
    30361 ggtgcgagag ctggaaatca aagaagcggg gaaccgtggc cgtccggcgg tggggctggt
    30421 ggttgaaact gaagcctggc actatctttc tctgcgcatt agtcgcgggg agattttcct
    30481 tgctctgcgc gatctgagca gcaaactggt ggtggaagag tcgcaggaac tggcgttaaa
    30541 agatgacttg ccattgctgg atcgtatcat ttcccatatc gatcagtttt ttatccgcca
    30601 ccagaaaaaa cttgagcgtc taacttcgat tgccataacc ctgccgggaa ttattgatac
    30661 ggaaaatggt attgtacatc gcatgccgtt ctacgaggat gtaaaagaga tgccgctcgg
    30721 cgaggcgctg gagcagcata ccggcgtacc ggtttatatt cagcatgata tcagcgcatg
    30781 gacgatggca gaggccttgt ttggtgcctc acgcagggcg cgcgatgtga ttcaggtggt
    30841 tatcgatcac aacgtggggg tgggcgtcat taccgatggt catctgctac acgcaggcag
    30901 cagtagtctc gtggaaatag gccacacaca ggtcgaccca tatggtaaac gctgttattg
    30961 cgggaaccac ggctgcctcg aaaccatcgc cagtgtggac agtattcttg agctggcaca
    31021 gctgcgtctc aatcaatcca tgagctcgat gttacatggg caaccgttaa ccgtggactc
    31081 attgtgtcag gcggcattgc gcggcgatct actggcaaaa gacatcatta tcggggtggg
    31141 cgcgcatgta gggcgcattc ttgccatcat ggtgaattta tttaacccac aaaaaatact
    31201 gattggctca ccgttaagta aagcggcaga tatcctcttc ccggtcatct cagacagcat
    31261 ccgtcagcag gcccttcctg cgtatagtca gcacattagc gttgagagta ctcagttttc
    31321 taaccagggc acgatggcag gcgctgcact ggtaaaagac gcgatgtata acggttcttt
    31381 gttgattcgt ctgttgcagg gttaacattt tttaactgtt ctaccaaaat ttgcgctatc
    31441 tcaatttggg ccaggaaagc ataacttaga ctttcaaggt taattatttt cctagtttat
    31501 atttgtgaag cataacggtg gagttagtga tgctgaagcg tttctttatt accggtacag
    31561 acacttctgt agggaaaacg gtggtttccc gcgcattgct acaagcgtta gcctcccagg
    31621 gaaaaagggt tgcgggatat aaacccgtag cgaaggggag caaagagaca cccgaagggc
    31681 tgcgtaataa agatgccttg gtgttgcaga gtgtttcaac catcgaactg ccttatgaag
    31741 cagttaatcc tatcgcgtta agcgaagaag aaagtagcgt ggcgcacagt tgcccaatca
    31801 attacaccct catttcaaac ggcctggcaa acctgaccga taaagtcgat catgtcgtgg
    31861 tagaagggac tggcggctgg cgcagtctga tgaatgattt gcgtccactc tctgaatggg
    31921 tagtgcagga acaactgccg gtgttgatgg ttgtcggtat tcaggaaggt tgcattaacc
    31981 atgcactgct aacagctcag gcgatcgcca acgacgggct gccgctcatt ggctgggtgg
    32041 ctaaccgaat caacccagga ctggcgcatt atgcggaaat cattgatgtg ctgggtaaaa
    32101 aacttccggc accgctcatt ggtgaactgc cttatctgcc gcgcgccgaa cagcgtgaac
    32161 tggggcaata catccgctta gctatgctgc gcagtgtgct ggcggtagat agagtcacgg
    32221 tgtaacgtcc gcgaaattac cgacgcaatt acgcaggcaa tcaataaacc ggggagtagc
    32281 tgatactccc cggtcatttc acatatcatc aacgtcgaca taatcggcgc gtgcgtggtt
    32341 gccgccaaca gtgtcgccat cccggtcaat ccgagtaaaa gcgtaatttc ttcgccatca
    32401 gggaaccata atcccaggct acgaccatac aacatgccaa tggcaagacc gataaatagc
    32461 gtcggtgtaa agacaccacc gggtgcgccg gaaccgctac tcgccagcac ggcacacagt
    32521 ttacaaagga agatcccggc aatgatcatt aacagtggtg gggcggttaa aaaggattgt
    32581 acggtgctat agccgttgcc ccacactgca ggtgtgaaca gggaaagcag acccacgatc
    32641 aacccgccta gtgccagttg ccagggcggc gcaagtttga gactcacaaa tccacgatga
    32701 caggcgttca ttaacgttaa caacagtggt ccgcacagac ctgccagcac acctgtactg
    32761 ataatcagcg catagtcacg agcctgaacc gtcactgaga gttgtacgtt gtagagtaac
    32821 gcgtcgctat gattaatcag attgctaacc agccatgcca cgatggcgga aataatcacc
    32881 gggccgagag aggccaacat catagtgcca aacagcactt cggctataaa taaactgcca
    32941 gcaagcgggg cacgataggc cgcagccatt cccgccgcgg ccccacaggc gatccataat
    33001 ttccattcct ggcgtggcgt aaaacgttgg gcaaaacagg aggcggcaag ggcagctaaa
    33061 agaatcatcg caccttcgcg accaattgca ctgccgctgg ttactaccag cagcgaggca
    33121 agcgatttaa ccaggcttgc tgcgtaatcg aactgtccat cggtttgcaa cgcttccatg
    33181 taatcggtcg gcgcatgagg gcgttgttgg gtaaatttct gccagcccat cagcaacaaa
    33241 cccgccgcca gtccgccgag cgccggagtt agcagccgtc gccaggagga aaggtttgtc
    33301 gctgcattga ccagactgcc ggagtcatta ttgaggaaca accactccag tagcagcatc
    33361 gcatgacgaa acccggcaac ggcaaagaca gcgagaatac cgacgactgt tgcgataagc
    33421 agacggcgga acatagtgcg cagatccgga tagatgtgta gatgatgcat gagcagaaat
    33481 gggcaatgaa acacgggcga ctattttgcg gcaaaagagg ccaaccgcaa aagcagtgcg
    33541 tgctttaaga gataaaaaaa cgtgacattg taacgtatta ttgccgaatc gtgcgtaatc
    33601 gtcaccatcc ggcaatatta cggtgatcct atcgaaagag tggtttaacc gcgacaggaa
    33661 tcaacagttg tgattgccat tgcgccagcg ttaagcgagc cagttcaccc agtgcacggt
    33721 agaaagggtg ttctgctttt tcgataaaaa catcaagaaa acgtgttgac cacggaaaaa
    33781 ggtgccatgc cagcaattct tcgcattctg tctggcgacc attctccgcc aaccacgcag
    33841 ccatcaacag cagtgaacca aaatgatctt ccggttcgtt ttgcttcatt tcaaactgaa
    33901 tgcctttctc gcgcatccac tgacgaagtg ccaatgttga atcgccaaac agcacagatt
    33961 cgcgatccag ccagaccgac ccccacggcg gagacggcag tgcccacggg ccgacaaaca
    34021 aacgctgcca ggcctgggcg tgagtctctt cacactgtgt ctgaaaagcc gtcaccagcg
    34081 gcgctaatga cgcttctggt agaggccact gagtttccca gccgtcactg gtgagtaccg
    34141 caaccagagg tgcggcttcc gcactctctg gagcgtaata aaacagcgcg cccaacacgc
    34201 gcgccgcgac agaaaaatta tcttgctgtg aaaaatgggt cattcctaac atcctgaaat
    34261 gtgcgggttg ccccgcacca atttgttaac ctgcaatagc cattcctact gtcatgtgca
    34321 ggccataaaa gagcacacgc ccgatcatct caccaacaag tatgaggatt agccccagaa
    34381 caagtccggc aacgtgaggt tcacggcgac gaatgagcgg acaaagccag catccaagac
    34441 ctgcgcataa caataccaca cgccagacct gtaaggaggc gtaatctggt accagtgcgc
    34501 tggcttgttg cacggatgag tgaatagacg ccagagacaa accttgcagc acaatcacct
    34561 cggcacaagc gatcagcgcc agaacgctaa tgatggcaaa tggtgtggta ttaaatgtaa
    34621 cgcgggctgc ccgcagaatt gcggcagcaa gtattggacc gctcaacaat accgtcagga
    34681 agaaagccag cgtagtgtaa ccgttatgcc aggttggcac ggtgtcgatt tgatacacgc
    34741 aggtcatcat ccagacgaaa atgacgccga gcgccatgct gacaagtaac cagagtttcc
    34801 ccaacgcttg cggcatttta ccgatgaccg ccaccagcca ccatagtccg ccaacggcaa
    34861 aaaatatgga accggcagca atctcgttac tcagaccaga agctccgatt cgattcagcg
    34921 agttaaacgc tcgcagtggc gatcccaggt gcatgacaga ggcaataaac ccgatgccca
    34981 tcaacagcca gaggaaaaac atgccacgaa caatgtgctg acggtctgcg tcatttttgg
    35041 cggcgaacca gccaattccg ctaacaatca gcgcgcctac tacgcattga cccaatacgg
    35101 taaagataac cagtggccat tcatgccatc catttcccat tttacacttc ctccggatta
    35161 gccagataac cagtggtatc gccggtcggg cggctgttgg cgttaggttt gataacgata
    35221 ttgggttttg tgaaatgcgc gcgcggcagt ggcgcgacgg cagccagagt gccgtgcttc
    35281 tggcgcagct cttcaattgg accgaactca agtgcacgca gcgggcagga ttcgacgcat
    35341 atgggttgtt tcccctcggc gacgcgcgaa taacaaccat cgcacttcgt catgtgccct
    35401 ttttcagcat tgtactgtgg tgcgccgtac ggacaagcca tgtggcagta gcggcagccg
    35461 atacaaacat cttcatcaac caccacaaag ccatcttcac gcttatgcat tgcgccgctg
    35521 gggcagactt ttgtacatgc ggggtcatcg caatggttac aggagatgga gagataataa
    35581 gcgaacacgt tctggtgcca gatgccatta tcttcctgcc agtcgccgcc agcatattca
    35641 taaatacggc ggaaactgac ttccgggcca agatctttaa aatctttgca cgccagttcg
    35701 caggttttac agccagtaca gcggctggaa tcgataaaaa atccatattg tgtggtcatg
    35761 ggctactcct taaacctttt cgatctggac aagattgctg tgcgacgggt ttccctttgc
    35821 cagcggtgaa gggcggtgag aggtcagaat attgatactg ccgccgtgat cgacccggtc
    35881 accaaacata tccgctttaa gccacgcacc ttgcccgatg gcggtaacgc caggcagaat
    35941 acgcggagtc acttttgcgg caatcagcat ttctccatta ttgttaaata cccgcacggt
    36001 atcgccatga cggataccgc gtgcctgagc atcaatgggg ttgatccaca cctcttgtgg
    36061 gcaggcctgc tgtaacacat caatattgcc gtagctggag tgggtacgcg ctttggtttg
    36121 gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa attttgctga ggttgcgggt
    36181 tgggcgagga tcacatagcc catattccct gcagattcat gagagataag gtcttcttgc
    36241 tctgttggca tcaggtcagg caacaggata tcgcaatact tagccgaggc cgtcatgaag
    36301 tggtcaatgc caacaatcat ctcgcacttg ctgtcatcct gaagcacctc atgggtgtga
    36361 ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt agcaccataa aaacttgatg
    36421 gggacatcca gtttttcttt tccacgaaca ccatcacggg tcgcggtcat tttcgtacca
    36481 tggtcgatgg catctgtcca tgtaaaaacg gaaatctgcg ttttaacagg attctcgagc
    36541 atcgggaacc attctacccc cagatcccag ctaccttcgc gtacacctga gttgccgccg
    36601 tttatgccga cgttaccggt aagaacggaa agcatggcaa tagcgcggga cgtttgttcg
    36661 ccgttggagt gtcgttgtgg cccccaaccc tgacaaatat aagcaggttt tgctgaaccg
    36721 atctctcgtg ccaactggat aattttttct gccgggatgc tggtgatttt tgctgcccat
    36781 tccggcgttt tagctatgcc gtcaggccct tcgcccagaa tataggcttt ataatgcgcg
    36841 ttacgtggtg cgttggcggg cagcgttttt tcatcgtaac caacacaata tttgtcgaga
    36901 aatggctgat cgaccatgtt ttcagtaatc agtacccagg caatcgcaca ggccagtgcg
    36961 ccatcggtgc cagggcgaat gggcagccat tcatcttcac gcccggcagc tgtgtcgtta
    37021 tagcgtggat cgatgacgat catgcgtgcg tttgaacgtt cgcgggcttg ctcgacgtag
    37081 taagtgacac caccgccgct catccgcgtt tctgccgggt tatttccgaa cataacgacc
    37141 agtttcgtat tggcgatatc atccgggctg ttgccatcat tggcaccgaa catataactc
    37201 attgcggcac tgatctgtgc ggtactgtag ctgccatagc gactgagaaa acccccgcaa
    37261 gagttcatca gacggtacgg gacgttcgag ttggtgatat ttccgccatc tacgcctgtt
    37321 ccgtacagga catgtacagc ctcattgccg taatctttca ggatccgccg aagattatca
    37381 ctgatggtat ccagggcttc gtcccaactt atccgttcaa atttaccttc accgcgctcg
    37441 ccgacgcgct tcatgggata tttcaacctg tcaggatgat tcatccgtcg gcggatagag
    37501 cgcccgcgta aacatgcccg aacctgatga ttaccgtaaa cgtcgtcacc tgtcgtatca
    37561 gactccaccc agtacacggt gtcatctttc acatgcaaac gtagcagaca gcggctcccg
    37621 cagttaacgg tgcaggaact ccagaccgtt ttctcttcta ccggagcctc tgccgcccgg
    37681 accatttggg aaaatggcag agtgaaagca ctgcttgcca gcgcgagact gccaagtgcg
    37741 gaggttttca tcagacttct atggctgatt tcagccttca tgagcgcctc tgtggtatgg
    37801 attttcatca ttactcactt attgcttttc aaacaaaatg tcatgccaga atttatggtt
    37861 gtagtgggtt atattttttc gatctcgacc agattagtgt gctgcgggtt tcccttcgcc
    37921 agtggtgaag ggcgcagagt ggttagcgta ttcacacatc cgccatggtc gattttatcg
    37981 ccagacatat tgggctcgtg ccaggctccc tggcccatag cgctaactcc agggagaatg
    38041 cgtggtgtta ctttggctgg tagccgaact tcgccacgat ggttaaacac ccgcaccata
    38101 tcgccgttgg caatcccacg tttctgtgca tctatagggt tgatccacac ctcctgacgg
    38161 caggctgcct tcaggacatc aatgttgccg taggtcgagt gagtacggga tttgtaatgg
    38221 aaaccaaaca gttgcagtgg gaaggttcta cgttcagggg agtcccagcc ttcaaaggtt
    38281 gaggcataaa ctggcagtgg gcttatcact tcatcttttt ccagttccca ggtacgggca
    38341 atttccgcca gcttgctgga ataaatttca atcttaccgg aaggcgtttt aagtggattt
    38401 gcctcggggt cgtcacgaaa tgctttgtag gcgacaaaat ggccattggg atctttacgc
    38461 ttatagatac ccattttttt cagttcgtcg taagacggta acgccggatc tttggcaagc
    38521 attttggcgt acagatgttg taaccattgt tcctgcgtgc gaccttctgt gaacttttga
    38581 tagacgtcag gtccaagacg tttcgcgact tcactcagga tccagtaaat cggtttacgt
    38641 tcgaattttt cgctggtgac tggctggagg aaaatgagat atcccatgtt accggcgtag
    38701 tcgttaggaa taatatcttc ctgctcaacg gtcatcaggt cttccagcag aatgtcggca
    38761 tattttgccg atgaggtcat aaagttttcg atgaccacaa tcatttcgca tttcgattcg
    38821 tcctgcagaa tttcatgcgt tttgttgatg tcagaatgct gattaacgag ggtatttccc
    38881 gcgtagttcc agatggactc cattgccaga cggatggatg gacttgaagt agttgactca
    38941 atgctactta aggtcctgat atgactgaca ttaatcatga taacactgta tatccatcca
    39001 gttctttctc tgggtctccc tatcggtatc agcaagtcta cacctcaagc cctgacacaa
    39061 ggctgacaca aatcaatcta ctcagcgtag ctaagcctca gttagtccgc agagccaatg
    39121 gtcgatatac tatccgcttc agactcaagg gacagaccac accgtttctt tcagtatcga
    39181 cccgcagcac agacaggaga gtagccacaa tgcgtcagag agagcttgca gccaccgcta
    39241 aggccttcat gttggatagg cctgaagtct cactacagga gcttacagag catctccggt
    39301 ctatggctga gcagttcctg acagacgcta gtgacgatta ctggaatgga cttgaggtcg
    39361 ctactctggt cgacgagaag tccaacctga aggagctagc cgctacgcaa gccttgagtc
    39421 tggaccagca gaagggcata aggttagcgc tggaagtcct gaccgcagca cagcagagag
    39481 tcgacactgg tgatacctct ggtctgatta agctgattga tgataataac cttactgatg
    39541 actcaactat aggcgactct acgtcaattc tgaataatga gcagggggat agaccggcag
    39601 ttttcacaca ggagcgccag tcatctgtag ttttttcaag cctagtgtcc tcgctgctgg
    39661 ctgagaaggt ccagaccctg aagacatcca gctacaagga cctctcgtcc tcactcaata
    39721 ctgtctcccg gttcctgcct gaagacatgg acctgatgtc ccgctctggg tggctggctg
    39781 tcagggactc tatgctggct tctgaggtga gaccatcgac aatcaacaag ctgctgacca
    39841 aagcgaagat gtgtctcgat tatggcctga tgaatgggca acttgagggc cgcaacccta
    39901 ttgaacggat gaagctgacc aaggatatcg actccaagcg cagagcgttc acagacgagg
    39961 agctggagag acttctggtc cgtgttgagt
//
LOCUS       DAZXUL010000028        39514 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZXUL010000028 DAZXUL010000000
VERSION     DAZXUL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39514
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.028"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(127..3573)
                     /gene="mfd"
                     /locus_tag="ACH3L1_001384"
     CDS             complement(127..3573)
                     /gene="mfd"
                     /locus_tag="ACH3L1_001384"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFT4076185.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRIAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(3701..4774)
                     /locus_tag="ACH3L1_001385"
     CDS             complement(3701..4774)
                     /locus_tag="ACH3L1_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFT4076186.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            5036..6235
                     /gene="lolC"
                     /locus_tag="ACH3L1_001386"
     CDS             5036..6235
                     /gene="lolC"
                     /locus_tag="ACH3L1_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFT4076187.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            6228..6929
                     /gene="lolD"
                     /locus_tag="ACH3L1_001387"
     CDS             6228..6929
                     /gene="lolD"
                     /locus_tag="ACH3L1_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFT4076188.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            6929..8173
                     /gene="lolE"
                     /locus_tag="ACH3L1_001388"
     CDS             6929..8173
                     /gene="lolE"
                     /locus_tag="ACH3L1_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFT4076189.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            8202..9113
                     /gene="nagK"
                     /locus_tag="ACH3L1_001389"
     CDS             8202..9113
                     /gene="nagK"
                     /locus_tag="ACH3L1_001389"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFT4076190.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            9129..9950
                     /gene="cobB"
                     /locus_tag="ACH3L1_001390"
     CDS             9129..9950
                     /gene="cobB"
                     /locus_tag="ACH3L1_001390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFT4076191.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(10106..11152)
                     /gene="potD"
                     /locus_tag="ACH3L1_001391"
     CDS             complement(10106..11152)
                     /gene="potD"
                     /locus_tag="ACH3L1_001391"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFT4076192.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(11149..11943)
                     /gene="potC"
                     /locus_tag="ACH3L1_001392"
     CDS             complement(11149..11943)
                     /gene="potC"
                     /locus_tag="ACH3L1_001392"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFT4076193.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(11940..12797)
                     /gene="potB"
                     /locus_tag="ACH3L1_001393"
     CDS             complement(11940..12797)
                     /gene="potB"
                     /locus_tag="ACH3L1_001393"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFT4076194.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(12781..13917)
                     /gene="potA"
                     /locus_tag="ACH3L1_001394"
     CDS             complement(12781..13917)
                     /gene="potA"
                     /locus_tag="ACH3L1_001394"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFT4076195.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            14167..15393
                     /gene="pepT"
                     /locus_tag="ACH3L1_001395"
     CDS             14167..15393
                     /gene="pepT"
                     /locus_tag="ACH3L1_001395"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFT4076196.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(15442..16563)
                     /gene="roxA"
                     /locus_tag="ACH3L1_001396"
                     /gene_synonym="ycfD"
     CDS             complement(15442..16563)
                     /gene="roxA"
                     /locus_tag="ACH3L1_001396"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFT4076197.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            16812..18041
                     /locus_tag="ACH3L1_001397"
     CDS             16812..18041
                     /locus_tag="ACH3L1_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4076198.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            18416..18604
                     /locus_tag="ACH3L1_001398"
     CDS             18416..18604
                     /locus_tag="ACH3L1_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4076199.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(18654..18983)
                     /locus_tag="ACH3L1_001399"
     CDS             complement(18654..18983)
                     /locus_tag="ACH3L1_001399"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076200.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(19108..19287)
                     /locus_tag="ACH3L1_001400"
     CDS             complement(19108..19287)
                     /locus_tag="ACH3L1_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076201.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            19417..19617
                     /locus_tag="ACH3L1_001401"
     CDS             19417..19617
                     /locus_tag="ACH3L1_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFT4076202.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19607..19828
                     /locus_tag="ACH3L1_001402"
     CDS             19607..19828
                     /locus_tag="ACH3L1_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076203.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            19821..20041
                     /locus_tag="ACH3L1_001403"
                     /pseudo
     CDS             19821..20041
                     /locus_tag="ACH3L1_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20043..20276
                     /locus_tag="ACH3L1_001404"
     CDS             20043..20276
                     /locus_tag="ACH3L1_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076204.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            20282..20581
                     /locus_tag="ACH3L1_001405"
     CDS             20282..20581
                     /locus_tag="ACH3L1_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFT4076205.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            20578..22326
                     /locus_tag="ACH3L1_001406"
     CDS             20578..22326
                     /locus_tag="ACH3L1_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFT4076206.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            22615..22893
                     /locus_tag="ACH3L1_001407"
     CDS             22615..22893
                     /locus_tag="ACH3L1_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076207.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            22890..23195
                     /locus_tag="ACH3L1_001408"
     CDS             22890..23195
                     /locus_tag="ACH3L1_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076208.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            23205..23279
                     /locus_tag="ACH3L1_001409"
                     /pseudo
     CDS             23205..23279
                     /locus_tag="ACH3L1_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23354..23467
                     /locus_tag="ACH3L1_001410"
     CDS             23354..23467
                     /locus_tag="ACH3L1_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFT4076209.1"
                     /translation="MSRVGISEPHHAAGAIRLFLRHLKRYGALLAFRHGPA"
     gene            23508..23732
                     /locus_tag="ACH3L1_001411"
     CDS             23508..23732
                     /locus_tag="ACH3L1_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076210.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            24033..25190
                     /locus_tag="ACH3L1_001412"
                     /pseudo
     CDS             24033..25190
                     /locus_tag="ACH3L1_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            25230..25802
                     /locus_tag="ACH3L1_001413"
     CDS             25230..25802
                     /locus_tag="ACH3L1_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFT4076211.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            25804..27015
                     /locus_tag="ACH3L1_001414"
     CDS             25804..27015
                     /locus_tag="ACH3L1_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFT4076212.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDAFSQAWKQ
                     EVKISEGEKPE"
     gene            27012..27350
                     /locus_tag="ACH3L1_001415"
     CDS             27012..27350
                     /locus_tag="ACH3L1_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFT4076213.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            27347..27643
                     /locus_tag="ACH3L1_001416"
     CDS             27347..27643
                     /locus_tag="ACH3L1_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFT4076214.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            27643..28083
                     /locus_tag="ACH3L1_001417"
     CDS             27643..28083
                     /locus_tag="ACH3L1_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFT4076215.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            28067..28249
                     /locus_tag="ACH3L1_001418"
     CDS             28067..28249
                     /locus_tag="ACH3L1_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076216.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            28305..28631
                     /locus_tag="ACH3L1_001419"
     CDS             28305..28631
                     /locus_tag="ACH3L1_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFT4076217.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            28615..30276
                     /locus_tag="ACH3L1_001420"
     CDS             28615..30276
                     /locus_tag="ACH3L1_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFT4076218.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            30290..30571
                     /locus_tag="ACH3L1_001421"
     CDS             30290..30571
                     /locus_tag="ACH3L1_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076219.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(31368..32828)
                     /gene="phoQ"
                     /locus_tag="ACH3L1_001422"
     CDS             complement(31368..32828)
                     /gene="phoQ"
                     /locus_tag="ACH3L1_001422"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFT4076220.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(32828..33499)
                     /gene="phoP"
                     /locus_tag="ACH3L1_001423"
     CDS             complement(32828..33499)
                     /gene="phoP"
                     /locus_tag="ACH3L1_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFT4076221.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(33668..35038)
                     /gene="purB"
                     /locus_tag="ACH3L1_001424"
     CDS             complement(33668..35038)
                     /gene="purB"
                     /locus_tag="ACH3L1_001424"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFT4076222.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(35042..35683)
                     /gene="hflD"
                     /locus_tag="ACH3L1_001425"
     CDS             complement(35042..35683)
                     /gene="hflD"
                     /locus_tag="ACH3L1_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFT4076223.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(35719..36825)
                     /gene="mnmA"
                     /locus_tag="ACH3L1_001426"
                     /gene_synonym="trmU"
     CDS             complement(35719..36825)
                     /gene="mnmA"
                     /locus_tag="ACH3L1_001426"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFT4076224.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(36879..37340)
                     /gene="nudJ"
                     /locus_tag="ACH3L1_001427"
     CDS             complement(36879..37340)
                     /gene="nudJ"
                     /locus_tag="ACH3L1_001427"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFT4076225.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(37350..38003)
                     /gene="rluE"
                     /locus_tag="ACH3L1_001428"
     CDS             complement(37350..38003)
                     /gene="rluE"
                     /locus_tag="ACH3L1_001428"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFT4076226.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            38175..39425
                     /gene="icd"
                     /locus_tag="ACH3L1_001429"
     CDS             38175..39425
                     /gene="icd"
                     /locus_tag="ACH3L1_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFT4076227.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT        10035 a         9756 c        10446 g         9277 t
ORIGIN      
        1 atataaggtt attgtaataa acagataagc ctgaattatg gatggtgaca gtgtcggata
       61 gtgcaggaaa gtgcaaagaa tttgtaaatg ttgcagatgg gggcgcagaa acgcccccga
      121 tttacattaa gcgatcgcgt tctcttccag ttcacgcata aactggcgta cccattcgat
      181 acgcgttttc cgctcactca aatcctgaat aaatttcagg cgcgtcggac catcaaggcg
      241 gtaatgctgc ggctgttttt gcagcaaacc aatcaaccag gccggattaa cgtgattctt
      301 ctcggcaaat tcgatcaccc cgcctttctc attaccttcc agcttcctga tccccagttt
      361 ctgcgcttgc tggcgcagac gggcaatatc cagcagggta cgcgccggat ctggcagcag
      421 gccgaagcga tcgataagct cgactttgat ctcttccagt tcgttttccg ttttggcgct
      481 ggcaatacgt ttgtagaacg acagacgcgt gttcacgtca gggatgaaat catctggcaa
      541 tagcgacggc atccgcagct cgacttctgt ttgctggctg gtgagatctt ccagcgacgg
      601 ctcgcgtccg gctttcagtg catcgacggc gttttccagc aactccatat acagcgagaa
      661 accgatggtt tccattgagc cgctttgttc ttcgccaagc agttcacccg cgccgcgaat
      721 ctccagatcg tgcgttgcca gcgcaaaacc tgccccgaga tcttccagcg aggcaatcgc
      781 ttcaagacgt ttttgtgcat cggtagtcat cgcttttgga tgcggcgtca gcagccatgc
      841 atatgcctga tgatgtgaac gtccgacgcg accgcgtaac tggtgcagct gcgccagacc
      901 gaagtgatcc gcacgttcaa tgataatggt gttggctgtc gggatgtcga tcccggtttc
      961 gataatggtg gtacaaacca gcacgttgaa acgttgatga tggaaatcat tcatcacccg
     1021 ttccagttcg cgctcgcgca tctgcccgtg accgatggca atccgtgctt caggcaccag
     1081 ttctgccagc cgttcggcgg ctttctgaat gttttccaca tcattgtaga gataataaac
     1141 ctgtcctccg cgcaaaattt cacgcaggat cgcctcccgg accaccaggc tgtcatactc
     1201 acggacaaag gttttaactg ccagacgacg ggcgggcggc gtggcgataa tcgacaggtc
     1261 acgcattccg ctcattgcca tattcagcgt acgtgggatc ggcgttgcag taagcgtcag
     1321 aatatccacg ttcgcgcgca tcgctttaat gcgctcttta tgacgcaccc cgaagcggtg
     1381 ttcttcatcg acaatcagca ggcctaaatc tttaaacttg acgtcacttt gcagcagttt
     1441 gtgcgtaccg atcagaatat cgattttccc ttccgccact tccgcaagga tttgcgtctg
     1501 ctctttggcg atgcggaaac gggagatcat ttcgatacgt accggccagt tggcgaaacg
     1561 gtcgcggaag ttgtcgtaat gctgctgcgc aagaagggtg gtaggcacca acaccgctac
     1621 ctgcttgtgg ttatctactg ccaggaaagc ggcgcgcatc gccacttctg ttttaccaaa
     1681 gccaacatcg ccgcacacca gacgatccat tgccagcggc tgacacatgt cgctaagtac
     1741 cgcattaatg gcctgcgcct gatccggcgt ggtttcaaac gggaagctgt cgcagaataa
     1801 ctgatactgc tcacgatcgt gtttaaacgc gaagccctct ttggcggcgc gttgcgcgta
     1861 gatatccagc aattccgccg ccacatcacg cactttttcc gccgctttct gccgcgcgcg
     1921 tgaccacgca tcgccgccaa gtttatgcag cggggcgttt tcttccgcgc cacctgcgta
     1981 acggctaatc agatgcagtg acgacaccgg aacatacagt ttggcgtcgt tggcataggt
     2041 gagcatcagg tactcgccag taatgccgcc agcttccagc gtggtcattc cggcatagcg
     2101 accgacgccg tgctccagat ggaccaccgg ctgaccaata tgcagctccg caaggttacg
     2161 gatcagtgta tcggggttga tggtgcggcg agaatcctga cgacggcggg caacacgttc
     2221 accgagcaga tcgctttcgc agatcagcgc cagattacgc gccttatcga caaaaccatg
     2281 ttcggcagcg ccaatcatca gataacgccc acggtcgctg gcttcatcaa gacgcataat
     2341 gcgttgcgga gcaattttaa ttcgtgcgag cagttcaccc agcgcttcac ggcgaccttc
     2401 actttctacc gagaacacca ccggaccgtc gaaagtctcg aggaacttac gcagcgcatc
     2461 cagcggcgct ttttgttgcg cctgaacggc caggtctggc agtttctgga aacctaaatt
     2521 ggcattcgcg gcttttgtcg gtaaatgttc agtttttagc tgcactcggg gccagttttt
     2581 cagctctgag aagagctcgt ccacccgcag ccagagcgat tgtggtggca acagcgggcg
     2641 catcggatcg acgccgcgat tctcaaaacg cgccagcgtg tcagcctgga aacgttcggc
     2701 actggtttcc agatcgccag tattcacgag caaggtattg gcagggaaat aactgaacag
     2761 cggtggcagt ggttcgctga agaacaatgg ctgccagtac tcgatcccag caggtaatgt
     2821 gcctttactc acttgctggt aaatatgttc cggatcgcgc ttcacttcga aggtatcgcg
     2881 ccactggctg cggaacagtt caattgccgc tttatcggtc ggaaattcgt gcgcgggcag
     2941 cagattgatc gcttctactt cctccagcgt gcgctggctg tcgacgtcaa acacccgcag
     3001 gctgtcgatt tcatcatcaa agaaatcaag acgataaggc agctcactcc ccatcgggaa
     3061 gagatccagc aacgcgccgc gcgtggcgta ttcgccgtgc tccatcacct ggtcaacatg
     3121 gcgataaccg gcgctgtcca gttgggttcg taatgcatct cgtgacaggc gctgaccttt
     3181 tttcatcacc agcgcatgac cgtggagaaa actgtgtggg caaacgcgct gcataagcgt
     3241 attcaccgga acaatcagta tgccacgctg catcgtcggt agctggtaaa gggtggaaag
     3301 gcgcgaggag ataatgtcct gatgaggcga aaaactgtcg tagggaagag tttcccagtc
     3361 cgccagattc atcaccatct gatcggtgaa ctggctgatt tcatcatgca aacgcagagc
     3421 attttgcata tctggtgcaa tgagtaccac cggaccggcg tgacgttcgg caatttccgc
     3481 taccagcgtt gcacaggctg cgccggttaa ctcgcccagc agacgctgct cacccgcttt
     3541 gacgggcagc gtataacgat attgttcagg cataacggtt gtcagattct cgttaggata
     3601 tgcctcaaca tatgggggca tatctctgat aagcaatgct gttcattatc cgtaatcgac
     3661 atggtttagc aaattgaatc gccccggctg gggtgatggt ttaacgagag taactgacgg
     3721 cagcgggcag tgaaggcggg gagaacaaca gatcgctgaa tgcacgctgt gaaagttttc
     3781 ttaccagcaa gcccgcaaca gtacagatga acaaactgac aaagggataa accagcagga
     3841 gtgtcagttc gacttgcggc gaccagcgtg ctgcgttcat ttgcgcaagc agagttaagc
     3901 tgaatatttc aaccagaatg cgatgggtgg tgtagatagc aatggtgttg gagccaatca
     3961 cattcagcag gctggtggag cgcataccga atcgttgctc gtactgataa aacagcttca
     4021 tgatcaccac aatcgatacc agcgacaaca gcagcgagat agtaaacaac caggcaccga
     4081 ccgccagaac ggtaagcaaa gaagccatca gcagatggcg gcgcaacggc acctctttaa
     4141 cacaggtcat tattgttgcg ccaaaccatg cgccaaggct gtaataaggc aaattgcgta
     4201 tcacactgtt cattccccac cacggcgtgg gaacgaaatt aaccgccaca ctcagcagta
     4261 caaacaaggc gaatagtggc agcgccagac ggctaaaaat tttacatacc acgaaataga
     4321 caattaacgc atacagatac cacaagctgg tgctggcggt gatcatcccg tgcaggaact
     4381 caccggtgga atcggcataa gcggcattgg aggcattgct taaatcgcgc tcaggtgcca
     4441 gccactggtt cagcgcactt aacgccagcc actgcaccac gccccaaagc gccagcaccc
     4501 agaagatgtt ccagatccgt ttatcgagac aatttcccca cggtacgctg tcgatatagc
     4561 ggcgaatcaa atagccggat ataaagaaaa aaaccggcat acgaaagggg gcaaggtaaa
     4621 gattgaaata gatccagcat ttgctcagga cttccgataa cggatgctga aaagtggtca
     4681 tatgcggata aaaggtaatg accgagtgat aaatcaccac cagacaaata cataaccctt
     4741 tgatctggtt aatccatagc tctttttgtt tcatcagaac aacacgctct tttttaattt
     4801 acatgcggtt gatcctgctg gttatgaccc gaggtgtaat gggtatctgt ctgtattgga
     4861 tgatttttca gattaagatc aggcggcaag attgatgata aaatatggca atttagccga
     4921 ttgatttacg ggggcttttc agattagccc tgacgatcac ttacagttca gacgtttacc
     4981 catcttgctt tcgcttatat actcgtgtct ttgctacagc aaccagacgg atttcatgta
     5041 ccaacctgtc gctctattta ttggcctgcg ttacatgcgt gggcgtgcag tggatcgctt
     5101 cggtcgtttc gtctcctggc tttctaccat cggcattacc ctcggggtga tggcgctggt
     5161 cacagtattg tcagtgatga acggctttga gcgcgagctg caaaacaaca tccttggcct
     5221 gatgccacag gcaattctct cttctgagca tggctctctt aacccgcagc aactcccgga
     5281 aacggcagtc aaactggacg gcgttaatcg cgtcgcacct attactaccg gtgatgtggt
     5341 actgcaaagc gcgcgcagcg tggcggtcgg ggtgatgctc ggtatcgacc cggcgcaaaa
     5401 agatccacta acgccgtatc tggtcaatgt gaaacaaact gacctcgagc cggggaaata
     5461 taatgtcatc ctcggcgaac aacttgcctc acagttgggc gtcaatcgcg gtgatcaaat
     5521 ccgcgtgatg gtgccatctg ccagccagtt cacgccgatg gggcgtattc caagtcagcg
     5581 cctgttcaat gtgattggca ctttcgccgc caacagtgaa gtcgatggct atgaaatgct
     5641 ggtgaatatt gaggatgcct cgcgtctgat gcgttatccg gcagccaata ttaccggctg
     5701 gcgtttgtgg ctggatgagc cgctgagagt tgactcttta agtcagcaaa aactgcctga
     5761 aggcagcaaa tggcaggact ggcgtgaccg taaaggcgag ctgttccagg ccgtacgcat
     5821 ggaaaaaaat atgatgggct tactgctgag cctgattgtc gctgttgcgg cgtttaacat
     5881 tattacctcg ctggggctga tggtgatgga gaagcagggc gaagtcgcga tcctgcaaac
     5941 gcaaggctta actccgcgac aaatcatgat ggtctttatg gtgcaagggg ctagcgccgg
     6001 gattatcggt gcaatcctcg gagcggcgct tggcgcactg cttgccagcc agttaaataa
     6061 tctgatgccg ataatcggcg tcctgcttga tggcgcggcg ctgccggtgg ctatcgaacc
     6121 tttacaggtc attgttattg cgctggtggc gatggctatc gcgctgctgt ctacgcttta
     6181 cccttcatgg cgcgctgccg ccacacaacc cgctgaggct ttacgttatg aataagatcc
     6241 tgttgcaatg cgacaacctg tgcaaacgct atcaggaagg cagtgtgcaa accgatgtgc
     6301 tgctcaatgt cagtttcagc gtgggcgaag gtgaaatgat ggcaattgtc ggtagctctg
     6361 gttccggtaa aagtaccttg ctgcacctgt taggcgggct ggatacgcca acctccggcg
     6421 atgtgatctt taacggtcag ccgatgagca aactgtcttc agcggcgaaa gcagaactgc
     6481 gcaaccagaa gctgggcttt atttatcagt ttcaccacct gctgccggat tttactgccc
     6541 tggaaaacgt ggctatgcca ctgctgattg gcaagaaaaa gcccgctgaa atcaacagct
     6601 gtgcactgga gatgttaaaa gcggtggggc tggagcatcg tgcgaatcac cgcccatctg
     6661 aactttctgg cggcgaacgc cagcgtgtag cgattgcccg tgcgctggtc aataatccgc
     6721 gtctggtact ggcggatgaa cctaccggta acctcgatgc gcgtaacgcc gacagcatct
     6781 tccagttgct tggggagttg aatcgcttgc agggcaccgc tttcctggtg gtcactcacg
     6841 acctgcaact ggcgaaacgt atgagccgcc aactggaaat gcgtgatgga cgtctgacgg
     6901 cggaactgag cctgatgggg gcggagtaat ggcgatgcct ttatcgttat tgattggcct
     6961 gcgttttagt cgcggacggc gacgcggcgg catggtgtcg ctaatctctg tcatttctac
     7021 cattggcatt gccctcggcg tggcggtatt gatcgtcggc ttaagcgcga tgaacggctt
     7081 tgaacgcgaa ctgaataacc gcattctggc ggtggtgccg catggcgaaa tcgaagcggt
     7141 aaatcagcca tggactaact ggcaggaagc actggataac gtgcagaaag tgccgggtat
     7201 tgccgccgct gcgccgtata tcaatttcac cggtctggtg gaaagtggag cgaatctgcg
     7261 cgcaatccag gtgaagggcg ttaacccaca acaggaacag cgtctgagcg cattaccctc
     7321 gtttgttcag ggggatgcgt ggcgcaattt taaagcgggc gaacagcaaa ttatcatcgg
     7381 caaaggcgtg gcggatgcgc tgaaagtgaa gcagggcgat tgggtgtcga tcatgatccc
     7441 caactcgaat cccgagcata aactgatgca gccaaaacgt gtgcgtttgc acattgccgg
     7501 tattttgcag ttgagtggtc aactcgatca cagttttgcc atgatcccgc tggcggatgc
     7561 ccaacaatat cttgatatgg gttccagcgt gtcaggtatt gcccttaaaa tgacggatgt
     7621 tttcaacgcc aataagctgg tacgcgatgc gggtgaagtg accaacagct atgtttatat
     7681 taaaagttgg attggtactt acggctatat gtatcgcgat atccagatga tccgcgccat
     7741 tatgtatctg gcgatggtac tggtgattgg cgtggcctgt ttcaacatcg tctccacctt
     7801 agtgatggcg gtgaaagaca agagtggcga tatcgcagta ttaagaacgc tgggggcgaa
     7861 agatggttta attcgcgcca tctttgtctg gtatggattg ctggcagggc tgttcggcag
     7921 cctgtgtggg gtggttatcg gcgtagttgt ttcactgcaa cttaccccga ttattgagtg
     7981 gattgaaaag ctgatcggtc atcagttcct ctccagcgat atttatttta ttgacttcct
     8041 gccatcggaa ttgcactggc tggacgtctt ctacgtactg gtcacagcat tgttgctgag
     8101 tcttttggca agttggtatc cggcgcggcg cgccagtaat attgaccctg cgcgagtcct
     8161 tagcggccag taaaggcagt acattaaaac aaggagcggc aatgtattac gggtttgata
     8221 ttggtggaac aaaaattgcg cttggcgtgt ttgatagcgg tcggcagttg cagtgggaaa
     8281 agcgggtgcc gacaccgcgt gacagctatg acgcattttt agatgcagtg tgtgagctgg
     8341 tagctgaagc tgaccaacgt tttggctgta aaggttctgt cggcatcggt attccgggaa
     8401 tgccggaaac agaagatggt aagctgtatg ccgctaatgt ccccgctgcc agcggcaaac
     8461 cgctgcgtgc cgacctgagc gcacgtcttg atcgcgatgt acgccttgat aacgatgcca
     8521 actgttttgc cctttcagaa gcctgggatg atgaatttac gcaatatcca ctggtgatgg
     8581 ggttaattct cggcaccggc gttggcggcg ggctggtttt caacggcaaa ccgattactg
     8641 gtaaaagcta cattaccggc gagtttggcc atatgcgtct gccggttgat gcgttaacca
     8701 tgatggggtt ggatttcccg ttacgccgct gcggctgtgg tcagcatggc tgcattgaaa
     8761 attatctgtc tggtcgcggt tttgcgtggc tgtatcaaca ctattatcat caaccgttgc
     8821 aggctcccga aattattgcg ctttatgatc aaggcgatga gcaggcaagg gcgcacgttg
     8881 agcgttatct ggatttatta gcggtttctc tgggaaatat cctgaccatt gttgaccctg
     8941 acctggtcgt cattggtggt ggcttatcga atttcccggc aatcacaacg caactggcgg
     9001 acaggttgcc ttgtcatctc ttacctgtag ctcgtgttcc acgcattgaa cgcgcgcgcc
     9061 acggtgatgc gggaggaatg cgtggtgcgg ccttcctaca tctaaccgat taaacaacag
     9121 aggttgctat gctgtcgcgt cggggtcatc ggttaagtcg ttttcgtaaa aataaacgtc
     9181 gcctgcgcga gcgtttgcgt cagcgtattt ttttcagaga taaagtggtg ccggaagcaa
     9241 tggaaaaacc aagagtactc gtactgacag gggcaggaat ttctgcggaa tcaggtattc
     9301 gtacctttcg cgccgcagat ggcctgtggg aagaacatcg ggttgaagat gtggcaacgc
     9361 cggaaggttt cgatcgcgat cctgaactgg tgcaagcgtt ttataacgcc cgtcgtcgac
     9421 agctgcagca gccagaaatt cagcctaacg ccgcgcatct tgcgctggct aaactgcaag
     9481 acgctctcgg cgatcgcttt ttgctggtaa cgcagaatat agacaacctg catgaacgcg
     9541 caggtaatac caatgtcatt catatgcatg gggaattgct gaaagttcgt tgttcgcaaa
     9601 gtggtcaggt tctcgactgg accggagacg ttacgccaga agataaatgc cattgttgcc
     9661 agttcccggc acccttgcgc ccgcacgtcg tttggtttgg cgaaatgcca ctcggcatgg
     9721 atgaaattta tatggcgttg tcgatggcgg atgtctttat tgccattggt acttccgggc
     9781 atgtctatcc ggcggctggg tttgtacacg aagccaaact acatggtgct cacaccgttg
     9841 aactgaatct tgaaccgagt caggttggta atgaatttgc cgagaaatat tacggcccgg
     9901 caagccaggt ggtgccagaa tttgttgaaa agttgctgga gggattataa agcaggaagc
     9961 attgcctgat gcgctacgct tatcaggcct acaagcggac cacaataccg acgaaagagc
    10021 gggataaaac tttactgtcc tgactcatta gccacatcct tgctaactaa aaaacgggcg
    10081 gtaataccac cgcccgcttg ctgaattaac gtcctgcttt cagcttctga taatactctt
    10141 cataaatgct gctggctgcg ccaacgtcat tctgccattc gccattttta atggtttcag
    10201 catccgggta gagcgtctta tcgtttgcca cttctggact taacagctta cgcgccgcaa
    10261 ggtttggcgt tggataaccg atagtttcag caacctgttt tgccacatcc gggcgcagca
    10321 ggaagttgat caatttcagc gcgccttctt tgtttttggc atttgccggg atcgccaggc
    10381 tgtccatcca gaatatgccg ccttctttcg gccacaccac gtcaataggc gtacccgcct
    10441 ggcgtgcaac gaaagcagaa ccgttccaga tcatgccgag gttaacttcg ccttccatgt
    10501 acgggttagc cgggttatcg gagttaaacg ctgcgacgtt tggcatcagt tttttcagct
    10561 cgttatatgc agcttcaatc tctttcggat cggtggtgtt accggagtag cccagcttac
    10621 gcagcgccat ctggaacact tcacgggcat cgtcggtcaa cagcaggctg cctttgtact
    10681 ctggcttcca cagatcggcc cagctggtga cagatttcgg atccaccgca tcaccgttaa
    10741 caccaatcgc cgtcgcaccc cagatatacg gaatggagta gtcgttattc gggtcaaaag
    10801 gcttgttgag catgtctgga tcgagattgc tgaagttcgt taacttcgac ttgtcgatct
    10861 tctggatcat cccctcttta cgcattttat cgacgtaata ggttgaagga accaccaggt
    10921 cataggcacc gtctttgtac gttttcagct tggcgtacat ggtttcgttc gactcgtaag
    10981 tcgaatagat aaccttaata ccggtttctt tggtgaactg ttcaagcagt cctggcggca
    11041 cgtactcggt ccagttgtag aaatacagcg tgttgttgtc atcggcgtga gcggcgctca
    11101 tgcccagtgc cagagcaccc gccgcgagca ggtggcgtga ccattttttc atttaacgtc
    11161 ccctgtgtta cctttcgttt tatcacgagc aataagctgg ctggcaataa ccatcaccag
    11221 cgacagcacc agtaatatgg ttgccagcgc gttaacttcc ggcgatacgc cgactttgac
    11281 catcgaataa atttttaacg gcagaatttc ataacttggt ccggtgacga atgaagaaac
    11341 taccacatcg tccatcgaca gggtaaagct taacacccag cccgccgcca ccgctggcat
    11401 tgccagtggc agaatgattt ttcgcagaat ggtaaattcg ctggcaccga gatctttcgc
    11461 cgcttccagc atccgcacgt caaaaccttt caggcgcgaa tacaccgtca ccaccacaaa
    11521 tggcaggcag aaggtgatat gcgagaacag cagcgaccag aagccaagct gaatacccag
    11581 cagcataaac agcaccagca gcgaaattgc catcacgata tctggtgaca tcattaccac
    11641 aaacagcatt ccgctaacga acggcttacc gcgaaagcga taacggtaca gcgcaactgc
    11701 cgtcagtgaa ccgataagcg tagcaaacgt cgccgaaaac accgccattg ttagtgaatg
    11761 ctgcgctgcc tgtaacaggc tgtcgttgtt catcagcagg ctataccatt tggtggtaaa
    11821 accctgccag ttgatgccaa agcgcgagct gttaaaggag ttcacaatca aaataatgat
    11881 tgggatatac aggtacgcgt agatagcggt cataaaaccg ccgcgaagca gtcgaccgat
    11941 cattcgagtt ccaccttctt attcagcaaa cgagaagcgc gccagtaaac cagcaacatc
    12001 aggcccatta cgatagtcag cgtaatgctg gtggctgcac caaacggcca gtcacgaata
    12061 ttgaggaact gtaccttgat gacgttaccg atcagcaggt ttttcgcacc gcccatcagg
    12121 tcggacacat agaacaggcc catcgctggc agcatcacca gcagacatcc ggcaataatc
    12181 cccggcatcg tcagtggaat aatgatgcgg ataaaagtct gtaacttgct ggcaccgaga
    12241 tcgcgcgccg cctcaagcaa cggcttatcc agtttttcga tactggagta cagcggcatc
    12301 accataaacg gcagcagaat gtaaaccaga ccgataatca ccgcactggg cgtgaacatg
    12361 atacggattg gtgtgtcgat aacgcccagc cagagcaaaa actcgttgag atagcctttg
    12421 gtgctgagga aaattttcag cccgtagata cgaatcaatg agttggtcca gaacggaaca
    12481 atcagcagaa acagcagcag cggacgcacc ttgtgtggca gcttcgccag aaaccaggca
    12541 aatggatagc ccagcaccag gcaggcgagg gtggcgatca gcgccatatt cagcgagtgc
    12601 aatagcactt caaaatagag cggatcgagc agacgcgtgt agttatccag tgtaaagacc
    12661 attttgacga aactggcgtc gtcgcgggtc aaaaagctgg tgccaatgat catcaggttg
    12721 ggcagaaaga caaacaacac aagccaaccg acaatagtga caatcactac attctggaac
    12781 ttacttgtgt tcttcatcag ccagtacgac ctcccagctt tctacccaat taatggccat
    12841 tttttggtcg agagagtggt caaagtcagg atcgtcttca ttgaagaatt cgctgaccat
    12901 caccatcttg ccattttcca gttcaacaac cgactccagc gtcatgcctt tgtagttacg
    12961 ctcacgaacg taaccaatca gcccttcagc gtggttgtcg tcgttaatct cttcaacacg
    13021 taagtcttcc gggcgcagca gaacatgcag tttttgcccc ggttcaacgg cgaagttaac
    13081 gtagatatta cattcgcggc cttcaacgtt ggcgcgtacg cgctgctcgt cgagtcgctc
    13141 gatcaccgtg gcgttaaaca tattaatttc gccaatgaac ccggcaacaa acaggttttt
    13201 cggctcttcg tagatttcac gcggcgtgcc gtcttgctca atgcgaccat cgcgcatcac
    13261 cacaatcctg tccgacatgg tgagtgcttc ttcctggtcg tgagtcacga aaacgaatgt
    13321 aatgccaagc ttacgctgta acgctttcag ctcgttctgc atttgcttac gcagtttgta
    13381 atccagcgct gagagcgact catccagcaa caacagacga ggcttgttaa ccaccgctcg
    13441 agcaatggcg acgcgttgct gttgaccacc agagagctga tgcggtttac gttgagcgaa
    13501 ggtttccagc tgcaccatcc gcagggcttc catcacgcgg ggcgtaattt cagcagcggg
    13561 ggttttttgc atgcgcaacc caaaggccac attttcgaac acggtcatgt gggggaaaag
    13621 tgcatagctt tggaaaacag tgttcacata gcggttttcc gccggaacgt gggtgatgtc
    13681 ctcgttatcc agcatgatgc gtccggaatc aacagtttcc agacctgcaa tcaggcgaag
    13741 aacggttgtt ttaccgcagc cagaagggcc aagcagcgtg aggaactcgc cattgttgat
    13801 agtcagatcc agctggggaa tgacctcttt accatcaaag catttgcgaa ttcccgccaa
    13861 ttgcaccagc ggtgaaagcg aactcggttg tttattcaat tttttactct gtcccatgta
    13921 aacgcaacgg atggcttacc gatgcggggt ttgtggttaa ccaccttggt gactcctaat
    13981 gagggcggta attctacggc aaaccgcttg aatcgccaat ctttgttgtg aattactggc
    14041 ttagctttat attcattaag gtaatgctga taaatattcc cgcttgcagg ggtaaaagtg
    14101 acctgacgca atatttgtct tttcttgctt cttaataatg ttgtcacaaa aagtgagggt
    14161 gactacatgg ataaactact tgagcgattt ttgaactacg tgtctctgga tacccaatca
    14221 aaagcagggg tgagacaggt tcccagcacg gaaggccaat ggaagttatt gcatctgctg
    14281 aaagagcagc tcgaagaaat ggggcttatc aatgtgacct taagtgagaa gggcactttg
    14341 atggcgacgt taccggctaa cgtccctggc gatatcccgg cgattggctt tatttctcat
    14401 gtggatacct caccggattg cagcggcaaa aatgttaatc cgcaaattgt tgaaaactat
    14461 cgcggtggcg atattgcgct gggtatcggc gatgaagttt tatcaccggt tatgttcccg
    14521 gtgctgcatc agctactggg tcagacgttg attaccaccg atggtaaaac cttgttaggt
    14581 gctgatgaca aagcaggtat tgcagaaatc atgaccgcgc tggcggtatt gcaacagaaa
    14641 aacattccgc atggtgatat tcgcgtcgcc tttaccccgg atgaagaagt gggcaaaggg
    14701 gcgaaacatt ttgatgttga tgccttcgat gcccgctggg cttacactgt tgacggtggt
    14761 ggcgtaggcg aactggagtt tgaaaacttc aacgccgcat cggtcaatat aaaaattgtc
    14821 ggtaacaatg ttcatccggg cacggcgaaa ggagtgatgg taaatgcgct gtcgctggcg
    14881 gcacgtattc atgcggaagt tccggcggat gaaagcccgg aaatgacaga aggctatgaa
    14941 ggtttctatc acctggcgag catgaaaggc accgttgaac gggccgatat gcactacatc
    15001 atccgtgatt tcgaccgtaa acagtttgaa gcgcgtaaac gtaaaatgat ggagatcgcc
    15061 aaaaaagtgg gcaaagggtt acatcctgat tgctacattg aattggtgat tgaagacagt
    15121 tactacaata tgcgcgagaa agtggttgag catccgcata ttctcgatat cgcccagcag
    15181 gcgatgcgtg actgcgatat tgaaccggaa ctgaaaccga tccgcggcgg taccgacggc
    15241 gcgcagttgt cgtttatggg attaccttgc ccgaacctgt tcactggcgg ttacaactat
    15301 catggtaagc atgagtttgt gattctggaa ggtatggaaa aagcggtgca ggtgatcgtc
    15361 cgtattgccg agttaacggc gcaacggaag taagcgaaaa gggatgcggc atgtgatgct
    15421 gcatccggct taaatccaaa cttacccttc gaagaaccaa tacccgctat tgaccagcgc
    15481 cgcgagcatc gcgaggaatg acggatcttc cagcgcatcg ccaaaattct ccgcagtcag
    15541 cgcaatgttg ctggcgagtg catccagtgc cggacggtgc ggggaatcga tcttctcacc
    15601 attggcatac acgtcgtcgc caatgcgcaa tacgcgcaga ccacccaggc gcaccagcac
    15661 atcaccttgt ttcagcgcat cgtagatttc atctggctga taaggcggtt ccggcggcgc
    15721 gatatccagt tcatgacgtg actgggatat aaactcgcca aaccattgct taaagtgttc
    15781 cggctggttg atcaattcga gcatcatctc acgcagttta tccatctctt gcggcagaac
    15841 atccgcagga tgagcacgag gtggaacatc cggatcgctg tagtagttgc cgcccagttc
    15901 acgttgcagc acataatcgg caaatccgct aattaattcc cgcgtatttg gcgcgcgaaa
    15961 acccacggaa tagttcatcg cattttccag cgcgtagcct tcatgcggga atcctggcgg
    16021 aatataaaga atatcaccag gctccagctc ttcatcgatg atggcttcga acggatcgac
    16081 ctgtaacaga tccggatgtg ggcagtgctg tttcatttgc agcttttcgc ccactcgcca
    16141 gcgacgacgt ccggtaccct gaatgataaa cacgtcgtac tgatcgagat gcgggccgac
    16201 gccgccgcca ggtacagaaa aagaaatcat cagatcatca atacgccagt ccggtagttc
    16261 acggaacggt cgcatcagcg cggcggtcgg ctcatgccag tggtttactg cctgtaccag
    16321 taatgaccag ttggtttcac cgagatgatc gtagctttcg aacgggccgt ggctgacctg
    16381 ccatttgcca tcctggtgac tgaccagtcg actgtcaact tcgctttcca tcgccagacc
    16441 cgccaactcg tctggagaga tcgggtcaat aaaattatta aagccgcgtt ttaacaccac
    16501 cgggcgtttc tgccagtgac gttcaagaaa atcgggccag ttaagagtga gttggtattc
    16561 catgttaagc atccacaggc tggtatctgc aaccgattat aacggatgct taacgtaatg
    16621 cgtgaagtat ggacatattt tatcaattag tgttttttgc cgtttcataa tgtttcagat
    16681 tgttcattat gtttttgatt ataaacagat ttgtgatttt ccttgttcca gcttgtttca
    16741 cgttgtagcc ctttgtgcaa cgatcggtgt agttaatggt gtagttaatt gtgatgaccg
    16801 gaatcaaaat tatgagcaga gcacttaaca aactgagcga tacacagctg aggaaaatca
    16861 acggcacacc cgcccaaaaa acagcctttc ttaatgacgg tggaaacctg agcgtcaggc
    16921 attcaaccag tggcctttta acctggtatt tcacttacag ggccggaacg ggaagggggg
    16981 caccaccgga acgcattaag ctgggaaatt atcctgatct gagcctgaaa tcagccaggg
    17041 aaaaagccgc ccagtgtcgc gcatggctgg cagaggggaa aaatccacgt catgagctta
    17101 attacaccgt acaggaagcg ttaaagccgg taacggttgg cgatgcgctc acctactggc
    17161 ttgagtcgta cgcaaaggaa aaccgcgtgg attatgccgc cctgaaaaag cgccttaata
    17221 atcacgtaat acagcacatt ggtgctatgc cgctggataa atgcgagcta cggcactggc
    17281 tggcctgttt tgaccaggtg gcaaagcgaa cgcctgttac tgccggattc ttgctacaga
    17341 cgtgcaaaca ggcgcttaag ttctgccgga ggcggcgcta tgcaatcagc aacgttcttg
    17401 atgatatgag tgtggcggac gttgggaaaa aaccggatat aagcgagcgt gtcttaagca
    17461 ccaaagaact gggcgaatta ttgcaggcac tggacaaaaa aatattctcc ccctactaca
    17521 tcgcgttaat ccgcctcctg attgtgttcg gatgccggac ggtagaactg aggttatcgg
    17581 agatcagcga gtgggatttt accgaaatgc tctggaccgt gccgaaagaa cacagcaaaa
    17641 cgaaggtggc aatattccgg cccataccgg aagcaatact gccgttcgtc acgcagctgg
    17701 tggagcagaa caggcacacg ggcttattgc tgggggaagt gaaacaggaa acaagcgtgt
    17761 cgcagtacgg cagattagcg cacaggaggc taaatcaccc tcactggtca ctacatgaca
    17821 tccggcgcac ctttacaacg atgctgaacg atttaggcgt ggatcctcac gtcgtggagc
    17881 agcttacagg ccaccagatg ccaggaatgc agcgagttta taatcattcc cgttatctgg
    17941 atgctaaacg caatgcgctg gatatgtgga cggagcggtt agggatactg gcgggaacac
    18001 atgaaaacgt aaccacgcta ccagtagcca gaagaaaata atttttttcg tgttttttca
    18061 gtatgcgcat actggatatg cgaacagata caacgtgcaa caatgagcaa caatgcggaa
    18121 caactacgaa caagaggtga aaaagtgtac agatttataa gtagctgatt tttaatgtgt
    18181 tactgatttt ttatacacta gcgaatcact ctttaaatag cgagaaaaaa ggcatgaaac
    18241 aatattttat gcctttgtat taacatgaat ttaaaggatt ttatcctgat tattcctctt
    18301 tctgtaaacc atacggcctc aacatttgcc aactctgaat catcctgaca caatcagaca
    18361 catcccaacg caatagaaca cgaaataact ctttaagaaa cgaaaggggg caatagtgtt
    18421 aagcactgat cggtttatac gtgaaaaaga atgcgaaaag ctaacaggcc ttagccgctc
    18481 atgccgctac cgcctggaaa aggccggaca attcccatca cgtcgtaaac ttggcggtcg
    18541 ttccgttggc tggtctttat ccgaggttct ggcctggaag gatagctgca aggcagttca
    18601 ttaatcacgc tggcggcacg cagccgccac acatcaatca tctgaacaca gagctataac
    18661 catgaagatt gaatatacgc cagaacgtgg gcggggattc gttcgccctg gtgaaactgg
    18721 aaaaccacaa aattggggtt tttcaggcat aaaaaaagcg gccccgaaat ggagccgcct
    18781 ttctgaacag ataacccgct gcgccgtttg tgtgtgtgat cccaaacata agcacgggga
    18841 tgatagccgc tatcaggctg gtgggcaatg caatcagtct ggttcagtgc gttgccataa
    18901 ctgcgaagtt ctctcctttg atgagtattc tttaaggtac ttctcaaggg caaaagaaca
    18961 cggcgcgaat cgttctgatt catgctcttt ctttctgcgt cgtctttttc gtgcaggtga
    19021 tgttttttcg gttgattctt cgttggtcat tgtgtgcgcc tgtaaagcaa tgcgccgtag
    19081 tacctcacac cacggcgctg atagttttta tcctttgggt tctatgccgc gttgctgtaa
    19141 ttctttgcgg attattcgct ttatccaggc agcaagagaa tcatcgccgt cctgttgttg
    19201 tgccttttcc atcttttccc gtaagtcagg atctaaacgg aactggaacg gaggattacc
    19261 acgcctttcg tttttgtgtg ttgacacgtt aattacacct caggtaatgt atttatgtgt
    19321 agtgacacat tacacacaat caacacaaaa agcaaagccc cgcactgtag gagcagtaac
    19381 ggggcttcta accaacaacg taaactagga gccgttatgg ttgccgtaaa tcatatacca
    19441 caccttgtac acacacaaac ggcctttgtg tggcgttttc tggcactgag tgccggagaa
    19501 tcccaaatca tccacgtaac cgcttggacg gaacgcgaag cgcgtagccg ttgcccgtcc
    19561 ggttgtgttg ctgtattcgc cgcccgtatt cgtcaggggg aaacctatgc acaataaaac
    19621 cacaccgaac gcagcagccg ccgcactcac tacgctgatg cacgcgctta ttgatattga
    19681 atgcacggca gagcttgcgc agggagaaga acagaaagac cggacacagt tcgccctgga
    19741 atgtatccga tacatcgcaa cgcggtcact gaatgacgct aaaaatattc ttgttgccga
    19801 ttgtgaaaat ggggggggtt atgcgtgatg atcgttttaa ttccctgaaa caggaatttt
    19861 ccggcgttcc tgatgatgcg gctgatgcgc tttcgtcaat gccagaactt attagagcgg
    19921 cttttttctt actttccacg agagaatata aatcaacggg cttgatgtac tgaatatcgc
    19981 cgccgattat gctgatttcg tgacagaggt tattttaaga aaaacgacgg acggggatta
    20041 atatgcgtga tatttaccac gaaacaatag accgcgcatt tcttgcactt tctcacagtg
    20101 aaaacatgct ggaaatattg cgcatatggc ttgaaacact tggcgacaat gaacgcgaca
    20161 aacaaaaatc aagaattgcc acggcattaa taacgcttct tgagcctgta ataatggaac
    20221 tgcaagaaat agatctattg cacgacagat ataaagaaca acacaccgga gaataaaaat
    20281 aatgaaactt aaatattctg gcttaactgc cagtggcaac actcacccta aatttacgcg
    20341 cggtgatatt taccgcgacc agtacggcgg catggtaacg attaaaggcg tggcagaacg
    20401 gcgcatcacc taccgccgtg aaggttacga atatgattgc gtgatgcctg tttatcaatt
    20461 ccggcgtgat ttcacgctgg tacaggcagc gccccgcagt aaaccaacca gcagggagaa
    20521 agcacgcgcc aatattcagg aaataaaaaa gatgcttaac gtattcaggg gtaaaaaatg
    20581 aaactggcac cgaacgtaaa aaaacagcca cgcggaataa aacacaaaga cacagaggta
    20641 attatctttg cgggtagtga tgcctggtcg cacgcgaaac aatggcagga gcaggatggc
    20701 cccgcatccg gcgataatgt gccgcctgtg tggcttgggc caaatcagct tgccgaactt
    20761 gatgcactga aaattgttcc ggatggaaaa aaacgcgtaa ggctgtacca ggccggagaa
    20821 ctggatttgg tggagaccaa aaagattggt cagaagctgg cggcggcaga tattcaggac
    20881 gcaaattttt accccgaagg aatgcacgtc cagaagtgtg aaaactggcg gcgctatctg
    20941 aatgctgagc gtgaaaatat tgccgcaggg cttaccatgc cggagcagaa aaatacgcaa
    21001 ctggcacaaa tggcagacag tgagcgcgcg cagctgcttg ccgagcgctt tgatggcgtt
    21061 tgtgtgcatc aggaaagtga aatcgttcac gtatggcgcg gcggggtatg gtgtccggtc
    21121 agcacaatgg aacttagccg cgaaatggtg gtgatctatt cagagcacag ggccactttc
    21181 agcaagcgcg taatcaataa cgccgtggaa gcgttaaaag ttattgccga accaatgggc
    21241 gagccgtccg gcgatttgct gccgttcgcc aatggtgcgc ttgacctgaa aacgggggaa
    21301 ttttccccgc acacgccgga gaactggatc accacgcaca acggcattga gtacacgcca
    21361 ccagcaccag gggagaacat ccgcgataac gcgctaaact ttcataaatg gcttgagcac
    21421 gcagccggaa aagaccagcg caagatgatg cgtatatgtg ccgcgctgta catgattatg
    21481 gcgaaccggt acgactggca gatgtttatt gaggccaccg gagacggcgg gagcggtaaa
    21541 agtacattca cacacatagc cagccttctg gcagggaaac agaacacggt aagcgctgaa
    21601 atgacatcgc ttgatgatgc tggtggacgt gcgcaggttg tcgggagtcg tcttatcgtc
    21661 ctggctgacc agccgaaata tacaggcgaa ggaacgggca tcaagaaaat cacgggcggt
    21721 gaccccgtgg aaattaaccc gaaatacgaa aagcgtttca cggctgtaat cagggcggtg
    21781 gtgctggcga ccaacaataa cccgatgata ttcaccgaac gggccggagg tgtggcacgt
    21841 cgtcgcgtga ttttccgttt cgacaatatc gttagtgagg ccgaaaaaga cagggagcta
    21901 ccggaaaaga ttgcggctga aatccctgtc attatccgcc gattgctggc gaactttacc
    21961 gactctgaga aagcacgggc tttactactg gaacagcgtg acggtgatga agctctggca
    22021 ataaagcaac agacggatcc ggttattgag ttttgccagt tcctgaattt tctggaggaa
    22081 gcacgcggcc tgatgatggg cggcggtggc gattcagtga agtacacgac cagaaacagc
    22141 ctttaccgcg tctatctggc gtttatggcg tacgcaggca ggagcaaacc gctaaacgta
    22201 aatgactttg gcaaggctat gaagccagcc gcgaaagttt acggacatga atatattacg
    22261 cggaaagtta agggagtaac gcagactaac gcaataacaa cagacgagtg cgacgcgttt
    22321 ttataatttt ttgtaattgc tgtctaccct gtctacctga gtaaagaaaa atacatttaa
    22381 ttcagtgcat taagttaggt agatagcctt tttttactgt ctacccacta tctaccctct
    22441 ctacctgatt ttacctgaat cagacaggga ggtagacacg gggtagatag tggataaaag
    22501 cactctaccc cgctgaaagc agcgccatta ctggcatgag ggccactaag gtagataagg
    22561 tagacaaggg gaggcacaac tcaaaacttt ttaaacgagg gggtaaaaat aaatatgcac
    22621 acttcaggga aatttaataa atcattcaaa aaacgcagag acagaacaga accgaaatat
    22681 cgcgcgttag acatgacaga gcacgcttta aaggtggcaa tcagaacgat agaccgccac
    22741 gcgggggaag gatacgcgaa agcacatccc gaactgataa gcgcattcat gaccacgacg
    22801 gcggcaaatt ttgccacgct gacagagcgg gagattgccg aagcggaaca ggtaacaaca
    22861 atcaacgtta aaaccggaga ggtggaatca tgagcgaacc aaaatttggt gaaaaatttt
    22921 acaagcacaa cggacggatc acgattctgc aaataagcgc agctacacca ggctggtggg
    22981 ttgaaacaga cgaaggatcc tcacccgtgg catcatggga gctttgtgtg cttagttacc
    23041 ctgaccgcga tgtgtatcag gatattctgc ctgtgatatc cactgacaaa ggcatgaaac
    23101 ccgtagacat caaaaacatg gggtttcagt gcgtaatgct cactgaaaaa atgatggaag
    23161 agatgaaaaa gaacaatgcc ggatcgcttc actgaggtgg caatatgaca gcacaaattg
    23221 cagcttacgg gcagctggtg ggcgacccgc tggtaaaaca gagcagcaag ggcacaccgg
    23281 agccgccaac ggagaactac gcagacctga gaaatgcagt aaatcgcact tacgcagaca
    23341 tggggctgag caaattagca gagtaggaat atcagaacca caccacgcag ccggagcaat
    23401 ccggcttttt ttgcgtcatt tgaaacggta tggagcacta ctggcttttc gtcacggtcc
    23461 ggcatagtta ctatctgaaa caaacagaca caacagagga aaacgcaatg ccgatgaaat
    23521 ttgatgagat acttaaacag cgtgataaat accatgctga caacatggag acgatgaata
    23581 tcactgatta ccgcgcattt ctggaaacgg gggcgctgat tgaaaaggat catcatggtt
    23641 ttgttcgttg cgcgctatcc ggtgaaatgc tggcagtaaa tcctgaacag acagatgcat
    23701 tgatagaatt tctgaaaggg atcagagact gagcacccat acagcacata cagccggagc
    23761 aatccggctt tttgttattt tttgtaaatt atttgttcgt ggttgttcca cgttgttcac
    23821 tgagcggatc ggcatatttt acccgaactg aatcatgatt attctcgccc gtggtgccag
    23881 gacgctgggg ccactttccc gcctgttaat gtgctcgcca atattcatta ccaggcggga
    23941 aaacgatcgg tgcgattact gatttcctta tgaaaaacgg ttgagtgttt gccgcgtcct
    24001 ggagttcctt acttaacccc aggacttttt ttatgcagag aataatcgaa ttacgccagc
    24061 agaaaaccgc cattaaaaat cagatgcgcg acatgctgga gaacgctgaa aaagaaaacc
    24121 gcagtcttaa cgatgctgag ggcgcaaaat ttgacgaatt acgcgctaaa gctgaatccc
    24181 tcgataaaga catttcccgc cttgaagcca ttgcagacga agagcgcagc aagccaggta
    24241 aaagcagcca gaccactgac cccgcagaac tacgtaacta cattctgacc ggtgaaaccc
    24301 gcgcattaag tacaggcgtt cccgctgatg gtggttatac cgttatccct gaactgaaca
    24361 ccgaaatcat gcgaatgctg gcggatgaat ccaccatgcg tcgcatctgt accgtgaaga
    24421 aaatcagcag caacgagttt aagcagcttg tttccgctgg cggtgcgacc gttaaccacg
    24481 gtgaagaggg taaggcacgc gaacagacca gcaccccgca gattaacgag gtgagcatta
    24541 agctgtatcc ggtctatgcg tacccgcgca ccacacagga aatcgtggat ttttccgatg
    24601 tggacatcct ttcatggctg acgggtgaga ttggcgacac cttcacggaa accgaagaaa
    24661 gcgatctggt tgtgggcgac ggtgacaaaa aagcaaaagg ttttttatcc gtaccccgtg
    24721 cagagaagaa cgacaaagag cgtgattttg gtacgttgca ggtaattaaa ccttccgaat
    24781 ctctggcgtg gacatctgcg gacccgctga tcgacctgaa atttgcatta cgtaaaaaat
    24841 accgcaaaaa cgcggtctga gtggttaact ccacgacggc ggcaaaactt cagaaggtga
    24901 agaacgcgaa cggtgattat atctggcgcg accgtttaca ggcgggtgat cctgatacgt
    24961 tgctgggcct tccggtcgaa tatctggagt ttatgcctga taacgttatt gccctgggtg
    25021 acttcaaacg cggttactac attgttgatc acgaaacagg tgttcgcacc agaccggaca
    25081 acctgacaga accaggattc atcaaaattt tcacgcaaaa atatttaggc ggtggcgtgg
    25141 tggattcgaa cgcgatcaag attctggcac tgccggaaga cgacgattaa cagcatacag
    25201 aaggggctaa aaagcccctt tagtgtttta tgggtgaaaa aattatgaag agtatggaaa
    25261 tccggtcatc ggaaatcacc accagcggag ccggtacgct gacgggctac gttgttcgct
    25321 gggataagct ttcagaactg ttatggggtg agttttacga aaaattccag cggggggcgt
    25381 ttactgaatg gcttgcggcg ggtaatgacg ttcgcggcct gtatgagcat gaccacagca
    25441 tgttactggg gcgcacccgt tccggcacgc tgaaactgga agaggacgaa acagggttac
    25501 gctttgaact gaccccaccg gataccagta cagggcgcga cgttatcgaa ctggttaagc
    25561 gtggggacat ctcggggatg agctttggct ttcgctcccg taaggatgta tgggatacca
    25621 caacagatcc atgcgtgcgc accgtgctgg tggcggaact gtacgaaatt accgttacat
    25681 cggtaccggc ttaccccgat tccggcgtgg agctggcccg ccgttccctg tatgagcagc
    25741 accccgaaaa aatgccgcgt gcggataatc gccgctggtg ggcggattta gcgggggtgt
    25801 gatatgtggc ctttcagacg aaaaaaagag cagcgcagca tgacgcttga tgaatttatg
    25861 gcgctggccg gcacatcgaa cacgggggcg ggtgagtacg tatcatctgg gacagcggaa
    25921 tcactccccg ccgtcatgaa cgccgtcacg gtcatctctg acgcggtggc taccatgccg
    25981 tgttacctgt acctggtacg caatgagaag gggaaggagg cccgcgaatg gcttgattct
    26041 catccggtcg atcacatcct caacgagcgc ccgaacgcgt ggcagacacc ctaccagttt
    26101 aagcgaatga tgatccgcta ctgcctgtta agtggtaatg cttatgcggt tattcagtgg
    26161 gggcgtgatg gttttccggc ggctttacat ccttacccgc cgcagtcggt gaacgtggag
    26221 cagacaggtg atcataactg gcgctactgc atcactgacg cctacaccgg aaacatccgc
    26281 aactatttac cgtgggaggt tctccacctt cgttactcca cggatgacgg ttttatgggg
    26341 cgctcacctg taaccatctg ccgcgaatcg ctggggcttg ggctggccca acaacgccac
    26401 ggcgcgagcg tgatgcgtga tggcatgatg gcggcagggg ttatcacgtc aggcgaatgg
    26461 ctggacggcg tgaagggcaa acaggcatta gccgcactgg aacgctacaa aggggccaga
    26521 aacgccggaa aaacgcccat ccttgaaggg gggatgagct atcagcagct gggcatgagt
    26581 aatcaggatg ccgaatggct ggcctcccgt cgcttcacca ttgaagacat cgcccgaatg
    26641 ttcaacgtct cgccgatttt tttgcaggaa tacagcaaca gcacctacag caacttcagc
    26701 gaggcaagcc gcgcatttct caccatgacg atgcgcccgt ggctggcgaa ctttgagcag
    26761 cagataaaaa acgccttgct ggtggcctcg cctgtacctg gtatccggta tcaggtggag
    26821 tttgacagcg cggacctgtt acgggccaca cctggcgaac gctttgccac ctatgagcgc
    26881 ggcataaaat ccgacgttat gtgtccgaac gaagcccgcg aacgtgaagg gctgtcaccg
    26941 cgtgatggtg gtgatgcgtt cagccaggca tggaaacagg aagtaaaaat cagcgaggga
    27001 gaaaaaccgg aatgaacata gggcgactgc gtgacaggat aacgattcag accctgaaac
    27061 agaccaggga tataaccggc gaaatactcg aaacgtggga ggacggtcac acactctggg
    27121 caagcgtgaa catggtcagc agcaaggagg ccatttcatc gggcgcagag ctggcgattg
    27181 gtaccgtaag gatctggata cggtaccgga aggacatcaa cgccaccagc cggataaagg
    27241 tcagtacggg gccgctggcg gggcgtgtac tgaatatcat cgggcagccg ctgccggatg
    27301 ccgccaggac acgccttgaa attctttgtc gtgagggcgc ggaaaaatga cagaagaact
    27361 tatcaccctg gaagaagtga aactccattg ccgcatcgat ggcgacgatg aagaccagtt
    27421 aatcagcgga tacattgccg catcgcttga ggcgtgccag atacacatag gcaggcgctt
    27481 tgatgacggg ctggcgttca cgccagccat aaagattggc tgcatgatgt ttatcgctca
    27541 cctgtatgaa aatcgccaga ttgtcgcgga taacgcaaaa acacgcgtac ccatgacgat
    27601 aggcgcactc tggacggctt accgtgatgt gggggggtac tgatgccgtg gcaaccatta
    27661 agacgatgca cagaaccagg ctgtaacagg cgcgttaaat ccggcaagtg tgaggagcac
    27721 aggcgggcgg catggcgtgc agaggatgcc agacgaggac accgccgcgc gcgtggatat
    27781 tccagacagt gggacaaata ccgcgccatg tatctgagta aaaacccgtt atgcgtgcga
    27841 tgccttgaga aggggatata tacgcccgcc gtggtggtgg atcacattat cccgattaat
    27901 ggcggtgatg atgttctctt ctggcccgaa tggaaccatc aaccattgtg ccaggcgtgt
    27961 cataaccaga aaacgaaatg gcttgatccg gcaacaaaaa gcaagcgtgc cgcaggtgga
    28021 tttcgtgaag aggaagaacg ggctgctaac cgcaataact ggatgtatga cgctgatgaa
    28081 tgagcgggaa caaaaccgcc ttatccgtgg acttataagg cagcgtgacg catggaagac
    28141 acaggaaaca gggcataaag ataaagcgtc aggacgcgca gaacgcatca cagcgaagcg
    28201 attaaccgac cgtgaccgcg aggttatgga atgtttccgc aatcgctgat gaggctgtat
    28261 gacggggtgg ggggcgtttt caggacaaac ccgaccccgc ctacctggat gatatcgccg
    28321 tgaagcagtg gcgggaaaaa tcgcggcagc ttgcggaacg gggagacctg acccccgccg
    28381 actggagcaa tctggaactg tattgcgtca actactccat ttaccggaaa gccgttgcag
    28441 accttgcggc gcgcgggttc agcattgtta acagtcaggg cggcgagagc agaaaccccg
    28501 cattaagcgc aaaatccgac gctgaaagag tgatgataaa aatggcctcc ttgctcggtt
    28561 ttgacccgat aagccgccgt aaaaatccac cggaaacaga agaagaggac gagcttgacc
    28621 gcctggaata agtacgcaga agacgtaaaa acgggcaaaa ttccggcctg taaacggtta
    28681 aaacaggctg ttaaacggta cttttcggac cttaaaagcc ccctttacac gttcgatcgt
    28741 gaggttgtgg agcggtttat tgccttttcc agggtgtgcc cgcacgtaaa aggaccgatg
    28801 cgtggcagac ccattgagct ggagccgtgg cagcagttcg cctttgcgtg catcctcggc
    28861 tttaaggtta aggccaccgg acggcgcaaa tacacgagcg cctttatcga agtgccgcgc
    28921 aaaaatgcga aatccacgac cgccgcgatt ctggctaact ggtttctgat tatggagaac
    28981 ggtcagcagg atatctacac cgcagcggtg agccgtgacc aggcgcggat cgtgtttgat
    29041 aatgcgcgtc agatgtgcct tttatcccga ccgttacgca ggcgggtgaa tattcaggcg
    29101 cataaggtga tacacccgaa aagcaacagc ctgttaaagc cgctggcagc aaaagcggca
    29161 accatcgagg ggacaaaccc cagtctttcc atcgtggacg aataccacct acaccccgat
    29221 aacggcgttt attccgcgct tgagctgggg atgggcgcac gtcccgaagg gctgttattt
    29281 gccattacca catcgggcag taacgtcgtt tcagcctgta aacagcacta tgattattgc
    29341 tgccagatcc tggacggcga agaggttaac gattcaattt ttgtactgat ttacgaactg
    29401 gacgacgaaa acgaggttga tgatccggcg atgtggataa aggcgaaccc taatattgat
    29461 gtttccgtgg atcgtgaaaa actggcctca accatccaga aagcgcgggg tattccgtcg
    29521 cagtgggtgg agatgatgac aaagcgattt aatatctggt gtcaggggac taccccgtgg
    29581 atgggtaatg gtgcatgggc tgagtgtgcc ggaacgttca cggaggaaga tttacacggt
    29641 caggagtgtt acgcggggct ggacttatca tcaaccagcg atatttccag cgtatgctat
    29701 gcctttccgg tcggtaaaac cattatgctg atttcccgtc attatctgcc ggagttccag
    29761 ctacagaacc ccgccaataa aaaccgcgct gtctatcgcc agtgggctaa agcgggctgg
    29821 atacgcacaa cgccaggtga ctgcattgat tacgaccgga tcagagatga catcatgcag
    29881 gatgcggaga aatttaatat caggctggtg ggctttgata cgtggaacgc cacgcatctc
    29941 aggacgcaat tacagggggc tggttttgag gtggagccgt tcccgcaaac ctacctcaga
    30001 ttcagtccgg cggcgaaatc gttcgaagtt tttgttaacc gcagggtgat tgtgcatcgt
    30061 ggcgatccgg tgttgtcctg gtcgatgagt aacgtcgtga tgcagagtga cgcgaacgcc
    30121 aatatcaagc cgaacaagaa aaaatcaccg aacaagatag acccgagcgt agcggcgctg
    30181 atggcgtttg gtacattcca ggcagagcat gaggattttg cttttgatat aagcgacagc
    30241 caccgccaga aactggaaga atttagcggg gtgtaatgag gtcagcagca tgacagaagc
    30301 cgaaatactg cgattaatcc gccgcgtctc tggaatcagc cagcaggctg acgaacagac
    30361 cacgcagccg gacagcgtga cagccgaaaa ttatgcgcgt gttgttgctg aggtgatgcg
    30421 ccgtgatggt atccagctta atgatgcgga tatgcgcgac atacggatcc gcgttctgga
    30481 aatgctggcc tacaatcgcc gcgttgaact gtatcgggag aaggagaaaa tcacgtacca
    30541 ctggaagaag ccggagcggt tacggcggta actcactgag attaaagcaa agcgcaaaat
    30601 tgcgctggct acatccctca catctgagga ctgttaacca gcaggccgga atatattcag
    30661 aaactacgaa attttcgcag tttaattact cgtcagctta atcgctaacc cgctctgata
    30721 ttttcggaaa cctcaatttg aggaagtcgg cgaggtaact gtttgatatt cccgatagcg
    30781 caaagatgcg cggtcgggca gaatgaccac cacctcaatc gtgacggaga gtgacaacca
    30841 gtgacacacc gtgacaaaaa gacggtttaa ccgttcatta aaaatcgacg tgattttgtg
    30901 ctttccggat gacgtggcca acgtcatttt ttagcgaaaa attctgatta aggtcactgg
    30961 ctgaacggtc ataatgacca cgcagaccat cgaggcaacc atagcgatca tcgttacggt
    31021 tggagctttt ccgccattgg tggaaaaggt gcacatctgg atcatcatta cggtgatcaa
    31081 gggtatgtaa aacccaccag cctgattaaa ggttaaccgg aaaaaaaatc cggtagcccc
    31141 gttatgggga tccccatatc gacattaacg cccctcatga attggtgcgc ttccccctgg
    31201 aaagattacc cgcctgtata tttcttgtgt ctatttgttc cacgttgttt catacagtgc
    31261 accgaacggt gtagttaatg gtgtagtcaa tttgcgattt ttggcacttt ttcaatgaaa
    31321 attgtttgat cgaatcaatg acttgatgta gtggtaaaag gacatattta ttcatctttc
    31381 ggcgcagaat gctggcgacc aaaaatcacc tccatccgcg caccgcccag catgctctct
    31441 ccggcgacga ttttaccctc atattgctcg gtgatttcgc gggctaccgc cagccctaca
    31501 ccttgcccag ggcgtaaagt atcaacccgt tgaccacggt cgaaaatgac ctctcgcttg
    31561 cttaatggaa taccggggcc atcatcctcg accacaatat agagatgctc gtcggtttgc
    31621 cttgcagaaa tttcgacaaa ctcgaggcaa tatttacagg cattatccag cacgttgccc
    31681 atcacctcga caaaatcgtt ctgctcaccg acaaagctga tctctggcga aatatcgaga
    31741 gagatattga cccctttgcg ttgatacact ttgttcagcg ctgaggtgag attgtccagc
    31801 agtggggcga ccggatgcag ctcgcggctg agcaatgtcc cgccgcgcat actggcacga
    31861 tgcaggtagt agccaatttg ctgtgaaatg cggctgattt gctccagcat taccggctca
    31921 gcatcactga cgctcatctt ttcactacgc agagaacgca gcgtactttg cagcaccgcc
    31981 agtggcgttt tcagactatg ggtcaggtcg gtgagcgtcg tacggtattt gtcgtaacgt
    32041 tcgcgttcac tttttaacaa tcggttcagg tttcgtacca gactggtcag ttctcgcgtt
    32101 gtggctggat tgagcaattc gcggttatgt tcttccagtt cgcggacttc ttttgccagg
    32161 gcttcgatgg ggcgtaaact ccaccaggcg gcgacccaca gcagcgggat cactaacagc
    32221 agattggctg agagcacata gataaaccag ctccagacca tataggaact ttttagctcc
    32281 accggaatgg tatccaccac cacaatggtt aattttggca tccgcgatgt tgccgggtag
    32341 acgtttaccg ccaccgagtg ggtcatctcc gcgtcgtcat catcttcccg cacttcctgc
    32401 aactgttgct gtatcgaatg atctccactc agcaagaggc tggtatcgtt aacatccgct
    32461 tcaatttcat gaaaaccatt cgatttcagc cagtcaggct ggatcatttt catcagccag
    32521 ggcacgtcac gttgcgccca taaaagctgc ccgttctcat cataaattag cgtcatggtg
    32581 gggctttgct tgtcgatatt ttcgggtaac tcgacatgca acttattgtt ttcccactgc
    32641 gcaagggtat agaacagatt gctctcgcca cgtaacagcc gaaacgtagt tttatcgaaa
    32701 ctgacgctat aaccgatcag cgcgaccatt ccgtaggcaa gcgaaagcac cagtactacc
    32761 gctgccgttg ccaacagaaa acgtacccgc agcgagagcg ggaaaaaaag acgcagtaat
    32821 tttttcatca gcgcaattcg aacaggtagc cctggccgcg aacggtggta atgacttctt
    32881 gcggatattg tgcctgaatt tttttgcgca gacgtcccat cagtacatca atggtatggc
    32941 tttcccgcag ctccgcatcc ggatagagtt gtagcattaa cgaatctttg ctgaccactt
    33001 tgccattatt gcgtatcaac gtttccataa tggtgtattc gaacgcggtc agtttgatca
    33061 cttcgtcatt aatagataat tcacggcgag agagatcaac ctgaaacggg gggagcgaaa
    33121 tgacctgtga agccagaccg ctattacgcc gcattaatgc ctgcattcgc gccatcacct
    33181 cttcaatatg aaacggttta gtcacgtaat catcagcacc ggcacttaat acttcgactt
    33241 tgtcctgcca gctttcacgg gcggttaata ccagaatcgg cagtgaaaca tcgttgctac
    33301 gccagcggcg aatcagtgac agaccgtcct cgtcaggcaa tccgagatcg acaatcgcaa
    33361 tatccggtaa atgttcattg agataataat cggcttcttt ggcatcttct gcgtcatcga
    33421 cctgatgacc agcatcctga atctgaactt taaggtggtg acgtaacaac gcattgtctt
    33481 caacaaccag tacgcgcatt tttatttctc cctgtcttaa ttattaaaat agtgtaacgc
    33541 gattatgtgt ttatgggggt aaacattaaa taaaccagcg gggaggggag gtaaagtgaa
    33601 aaaagaaaaa gtggataatc ttaataagca ggccggacag catcgccatc cggcactgat
    33661 ttgaggctta tttcagctca tcaaccatcg tgatagctcg accaatatag ttagccggcg
    33721 tcatcgcttt caggcgggct ttctcttctt ctggcaacgc cagaccatcg ataaactgct
    33781 tcatgccttc ggcgtcaacg cgcttaccgc gagtaagctc tttcagcttc tcgtacggtt
    33841 tttcgatgcc atagcgacgc ataactgtct ggattggttc tgccagcact tcccagttgt
    33901 gatccagctc atccagcaga tggtcacggt tcacttccag tttgctcacg cctttcaggg
    33961 tggattgata cgcaatcagc gcataaccga tacccacgcc gaggttacgc agcacggtgg
    34021 agtcggtcag gtcacgctgc cagcgggaaa ccggcagttt gcttgccaga tgctgcaata
    34081 ccgcgttgga aaggcccaga ttcccttcgg agttttcgaa gtcgatcggg ttaactttat
    34141 gcggcatggt ggaagaacca atctcaccag caatggtttt ctgtttgaag tggttaaggg
    34201 cgatataacc ccagacgtca cggtcaaagt cgatcagaat ggtgttgaag cgcgcaacgc
    34261 aatcaaacag ttcggcaatg tagtcgtgcg gttcaatctg ggtagtgtac gggttccatt
    34321 gaatacccag cgaggtgacg aactcttcgc tgaactgatg ccagtcaact tccgggtaag
    34381 cggcgatgtg ggcgttatag ttaccgaccg cgccgttgat tttgccgagg atctccacct
    34441 ggttaagctg gcggtgctgg cgctccatac ggtaggcgac gtttgccatc tctttaccga
    34501 tggttgacgg cgtggctggc tgaccgtggg tacgagacag cagcgggata tcgcggtact
    34561 gaacggcgag atctttaatg ccatcaatca gttgacgcca gtacggcagg atcacttcat
    34621 cacgcgcggt tttcagcatt aatgcgtggg agaggttatt gatatcttcc gaagtacagg
    34681 caaagtggat gaattcagaa accgcgtgca gttccgggat ctccgccact ttttctttca
    34741 ggaaatactc aaccgcttta acgtcgtggt tagtggtacg ctcgatggtt ttgatgcgtg
    34801 cggcatcttc ttcgctgaaa ttggcgacaa ttgcatcaag gtaaccgatt gcgtcggcag
    34861 caaaagcagg aacttccttg atcgctgcgt gcgcggccag tttttgcagc caacgtactt
    34921 caacttgtac acggaatttc agcaaaccat attcgctgaa aatcccgcgc agcgcgctga
    34981 ctttatcgcc gtagcgtcca tcgacagggg aaacggcggt cagtgaggat aattccatag
    35041 atcacaactc cggggttaaa tgagcaagaa tttgttttgc ctgagtggtc aggcgattac
    35101 gagaaaacat cagttgtaga cgtccgccgc cgacctggtg ccagagcacg gcggcgcgaa
    35161 tgcctgccag cagggttgcg cgaactttcg cctgcacttg tgggctttgc agtacagcag
    35221 gggaaccggt gacctgaatg cgcgggccaa gcgggctgat cacatcaaca tagatagcag
    35281 ccatcgcgct catcagcgtt tcggactgta aatcgaagtg ttcgagctgg cgttgcaggc
    35341 cgttgatccg gttgcccaga gtgtcgagcg cgcctttcgc tgaggagagt ttgcgctcaa
    35401 gcaccatcaa gctgagtgtg tagcgggtta attcggcgtt taagccctgg caactgctgg
    35461 cattgagcac gccgagcagg gtttccagcc cgacgcgcag gttggcttcg ctaccgccaa
    35521 aaaccgccag cgtcgagctg gggttcatat caataatact gttgagtgag acgtgtagcg
    35581 catcggcatc acaatgcccc tggtgagcga gttgttgcac caggcgtgcc gactgacaaa
    35641 taccggccag ggcgagggtg atgtcatagt aattctttgc cacgttcact gcttccttgt
    35701 tttaagtaaa gataataatc agaccggcag cggcagacgc tgctcaataa taccgccacc
    35761 gaggcacact tcaccgttat agaagacggc agactggccc ggcgtcacgg cggcaaccgg
    35821 ctcatcgaaa atcactttaa tgcgatcgtc gtccagcgcc ttgacggtgc acggaatgtc
    35881 ggtctggcga tagcgggttt ttaccgtgca acgcatagtg ccggtgaatg gttcgcgatc
    35941 gacccagtgc agctgctggg caatcaaccc gacagacatc agccgcgggt gttcatggcc
    36001 ctgagcgaca accagaatgt tgttttcgac gtctttgtcc accacatacc acggttcttc
    36061 ggtaccttct ttggtgccac cgatacccag acctttacgc tgaccgagag tgtgatacat
    36121 cagcccctgg tgctcaccaa tttcatcgcc atcgacggta atgattttgc ccggttgcgc
    36181 cgggagataa cggcccagga actcgcggaa tttacgttcg ccaatgaagc aaatgccggt
    36241 agagtctttt ttcttcgcgg tgaccagacc aagatcttca gcaatcttac gcacctgcgg
    36301 tttttccagt tcgcctaccg ggaacaggct ttgcgcaatc tgctcatggc tgagcgtata
    36361 aaggaagtag ctctggtctt tattgctgtc cagaccacgc agcaggcggc tcttgccatc
    36421 gacatcggcc cgacgtacgt aatgaccggt agcgatataa tcggcaccta aatcttcggc
    36481 ggcaaattcg aggaaggctt taaatttgat ctccttgttg cacagaatat ccggattcgg
    36541 cgtgcgaccg gctttatatt cggcaaggaa cagttcgaag acgttgtccc agtactcggc
    36601 agcaaagtta acggtgtgca gttcaatgcc gagcttgtcg cagaccgcct gggcatcagc
    36661 cagatccgct gctgctgtgc aatattcctc accgtcgtct tcttcccagt tcttcataaa
    36721 caggccttcg acctgatatc cctgttgttg caacagccag gcagaaacgg aggaatcgac
    36781 accgccggac atgccgacga ttactttttt tgcggtttca gacattggat cactcacgac
    36841 attgaacttc aaggcggcgt attctatcac gcagcctttt agatgacacc ctttgtaaaa
    36901 ggccagttaa aatcgccaat catctccagc ggataacgtt gcccgctttg ataacaacga
    36961 atactttccg ccaccagcgg cgaacgaaga tttgacgcct gtaaaatttc ttcggcgctg
    37021 acccaacggc agcagtcgat atcgctgtca tgaggctggg tcgggcatat ttgctcaagc
    37081 tcaatggcaa agaggaaacg caaaaacggc gttttatctg gcgcaatcca ctgatgcata
    37141 cgaataaagt gttgcggctg cgcgctgatg ccggtttctt cccacagttc acgggcggcg
    37201 gcttccacta aggtttcatc ggcttccaga tgcccggcag gttggttcca taacgcttta
    37261 ccattaatcg tctcttcaac gactaaaaat ttgccttctg cgtgcaccac gcaagcaacg
    37321 gtaacgtgcg gtttaaacat tttgcatcct taatctgtca cttctcgcca ttcgccattg
    37381 gcaagattat ccaaagagta atctcccatc gcatagcgaa tcagtcgcag cgtggggaaa
    37441 ccaacatggg cggtcatgcg gcgcacctgg cgattacgtc cttcatataa ggtgatcttc
    37501 agccagctgg tgggaatact tttgcgttca cgaattggtg gattccgcgg ccataaccac
    37561 gcgggttcgt caaccagctc cgcgcctgcg ggcagggtag ggccatcatt taaggttacg
    37621 ccattgcgca aggcttcaag tgcgtcttgt gtgggaatac cttccacctg cacataatag
    37681 atttttccgg tgcgtttacc cggctgggtt aaacgcgcct gcaacgcgcc gttattggtc
    37741 agcaccagca acccttcgct atcgcggtca aggcgacctg ctgcataaac gccctgaacc
    37801 gggatgaatt cttttaatgt tttgcgtccg gtttcatcgg tgaactgcgg aagaacatcg
    37861 tagggtttat tgaagaggat cacacgcgtg ggctggtttt catgtttacg cctggtagaa
    37921 cgttgcgagc tgaatcgctt aacctggtga tttctaaaag aagttttttg catggtattt
    37981 tcagagatta tgaattgccg cattatagcc taataacgcg catctttcat gacggcaaac
    38041 aatagggtag tattgacaag tcaattacaa atcattaaca aaaaattgct ctaaagcatc
    38101 cgtatcgcag gacgcaaacg catatgcaac gtggtggcag acgagcaaac cagtagcgct
    38161 cgaaggagag gtgaatggaa agtaaagtag ttgttccggc acaaggcaag aagatcaccc
    38221 tgcaaaacgg caaactcaac gttcctgaaa atccgattat cccttacatt gaaggtgatg
    38281 gaatcggtgt agatgtaacc ccagccatgc tgaaagtggt cgacgctgca gtcgagaaag
    38341 cctataaagg cgagcgtaaa atctcctgga tggaaattta caccggtgaa aaatccacac
    38401 aggtttatgg tcaggatgtc tggctgcctg ctgaaaccct tgatctgatt cgtgaatatc
    38461 gcgttgccat taaaggcccg ctgaccactc cggttggtgg cggtattcgt tctctcaacg
    38521 ttgccctgcg tcaggaactg gatctctaca tctgcctgcg tccggtacgt tactatcagg
    38581 gcactccaag cccggttaaa caccctgaac tgaccgatat ggttatcttc cgtgaaaact
    38641 cggaagacat ttatgcgggt atcgaatgga aagctgactc tgccgacgcc gagaaagtga
    38701 ttaaattcct gcgtgaagag atgggcgtga agaaaattcg cttcccggaa cattgcggta
    38761 tcggtattaa gccgtgttct gaagaaggca ccaaacgtct ggttcgtgca gcgatcgaat
    38821 acgcaattgc taacgatcgt gactctgtga ccctggtgca caaaggcaac atcatgaagt
    38881 tcaccgaagg cgcgtttaaa gactggggct accagctggc gcgtgaagaa tttggcggtg
    38941 aactgatcga cggcggcccg tggctgaaag ttaaaaaccc gaataccggc aaagagatcg
    39001 tcattaaaga cgtgattgct gatgcattcc tgcaacagat cctgctgcgt ccggctgaat
    39061 atgatgttat cgcctgtatg aacctgaacg gtgactacat ttctgacgct ctggcagcgc
    39121 aggttggcgg tatcggtatc gcccctggag caaacatcgg tgacgaatgc gccctgtttg
    39181 aagccaccca cggtactgcg ccgaaatacg ccggtcagga caaagtaaac cctggctcta
    39241 ttattctctc cgctgagatg atgttacgcc atatgggctg gactgaagcc gcagacctga
    39301 ttgttaaagg tatggaaggc gcgatcaacg ccaagaccgt aacctatgac ttcgagcgtc
    39361 tgatggaagg cgctaaactg ctgaaatgtt cagagtttgg tgatgcgatc atcaagaata
    39421 tgtaatcact acatgtgtta aatatttaaa cgggcgtata acacgcccgt tgttttattt
    39481 atgtggatat tattaatagc atatcgagca tatt
//
LOCUS       DAZXUL010000029        38961 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZXUL010000029 DAZXUL010000000
VERSION     DAZXUL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38961
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.029"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            152..907
                     /locus_tag="ACH3L1_001430"
     CDS             152..907
                     /locus_tag="ACH3L1_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HFT4076228.1"
                     /translation="MKNSINFILQGKGGVGKSFTTAILAQYFIDEKNMDNVVVGDTDP
                     VNTTTAKVKRLNADLIQITENSKVVQSKFDPMFESMLTNTQNTFVIDNGASTFLPLIQ
                     YFNDNCVMDMFNEVEQEVYIHTIIVGGQAQADTIEGFEDLVKLVKGTKVKIIVWINEF
                     QGVPALENIPLIKTKFIEKNKDVIAGVVVIQDRKSDAFASDIKNLTEKSLTLKEALES
                     EFFGLMAKSRLKRVFNDIYVQLDSIYNMQHTEA"
     gene            957..1634
                     /locus_tag="ACH3L1_001431"
     CDS             957..1634
                     /locus_tag="ACH3L1_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076229.1"
                     /translation="MINDKSFNIENIISDIFKETRLKISKDDPVLSIILMHEKILEHA
                     LTQLKNNNQIATERLSHDISSIRDAINALPDAIDEKTSELQHAAVALHDEFQESKGEI
                     KGSLEEARINATEKLAESAKELQLNITKVAEKTTETIESANKIISAIDTNLAEINKKA
                     LANYVNDIRSLEKKGESISKNIDTAINNAFKSSVKSFKFYCGAALFISTVLQFTMWGF
                     FLYKLLT"
     gene            complement(1953..2255)
                     /locus_tag="ACH3L1_001432"
     CDS             complement(1953..2255)
                     /locus_tag="ACH3L1_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcgAII protein"
                     /protein_id="HFT4076230.1"
                     /translation="MDTFDSEKFLSELDVLSKEIMPGCGLVFELYQRRLSAAIDKVVA
                     GLPEEQHAQAFELARQEFDYLSAEEIAEEIRRDSERGYCCHGFDRDCCPLGCGDLP"
     gene            complement(2331..2564)
                     /locus_tag="ACH3L1_001433"
     CDS             complement(2331..2564)
                     /locus_tag="ACH3L1_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076231.1"
                     /translation="MSSEVIKQIQKIQDRGIIIYSKFRAAEFDQDDVYRESYFLVVEF
                     NELIAENIIHDEKLVDQTACILHELRRIAIEGK"
     gene            complement(2992..3279)
                     /locus_tag="ACH3L1_001434"
     CDS             complement(2992..3279)
                     /locus_tag="ACH3L1_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076232.1"
                     /translation="MINEAKKIAVEMMFWTACPVDDDNCFDAIINASKIDREFEDKHA
                     IYLHPIYRDLLSTDQIKSALKKRAIKIISFKCDINEDSARKILMQTILRAV"
     gene            complement(3586..3879)
                     /locus_tag="ACH3L1_001435"
     CDS             complement(3586..3879)
                     /locus_tag="ACH3L1_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076233.1"
                     /translation="MKFTSTTNHVFTFERVTLCTIVLIHKDTGQQYVVIFTDNNKIRD
                     YKTGIVSQFGELKQSDIDLILFYRDEYEKYFDSLSNGEEYLSFKEYIGCIRGK"
     gene            complement(4426..4668)
                     /locus_tag="ACH3L1_001436"
     CDS             complement(4426..4668)
                     /locus_tag="ACH3L1_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4076234.1"
                     /translation="MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHN
                     QLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP"
     gene            complement(4665..4919)
                     /locus_tag="ACH3L1_001437"
     CDS             complement(4665..4919)
                     /locus_tag="ACH3L1_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076235.1"
                     /translation="MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTL
                     SELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYKESDA"
     gene            complement(4916..5458)
                     /locus_tag="ACH3L1_001438"
     CDS             complement(4916..5458)
                     /locus_tag="ACH3L1_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076236.1"
                     /translation="MNSFFEKYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKN
                     YSVHIRMEKDRFSVVGSVDSRSWRSPYHVCTLSKKRNPIDIAADIERKILVNASQEVL
                     QAIEYEKRQAAKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDCYDL
                     QIRGLSIDQLVKITGYLKQL"
     gene            complement(5557..6072)
                     /locus_tag="ACH3L1_001439"
     CDS             complement(5557..6072)
                     /locus_tag="ACH3L1_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFT4076237.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYKKAALKYHPDRNPLGAELM
                     KAVNAAFDFLMANLDKINQYQSTEKSAIYNYGDDLEKVLNVLSGLSGLVFEVIGNWVW
                     ISGETITHKETLKEIGCKWAPKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRK
                     ATGWQRVETRA"
     gene            complement(6160..6412)
                     /locus_tag="ACH3L1_001440"
                     /pseudo
     CDS             complement(6160..6412)
                     /locus_tag="ACH3L1_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553847.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6689..7039)
                     /locus_tag="ACH3L1_001441"
     CDS             complement(6689..7039)
                     /locus_tag="ACH3L1_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFT4076238.1"
                     /translation="MTATKNVKSQKNDYPASIDHNAAMIEELRADPDYANAYLANALE
                     EINEPGGLGGFLVALRQVIEARGGISEAARKSGLARQSIYRALSPNGNPTITTLAQLT
                     SVAGLQFTLSKSSH"
     gene            complement(7036..7371)
                     /locus_tag="ACH3L1_001442"
     CDS             complement(7036..7371)
                     /locus_tag="ACH3L1_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFT4076239.1"
                     /translation="MPYTAKRYQTENGEVPYTDWMKKLRRKDQTAALKVDSRIARAMG
                     GNFGDHKFERDGVWELRVDYGPGYRVYYSIEDGEIILLLIGGNKKTQTADLDKAVSYL
                     QDFKKRSKK"
     gene            7733..8392
                     /locus_tag="ACH3L1_001443"
     CDS             7733..8392
                     /locus_tag="ACH3L1_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFT4076240.1"
                     /translation="MGKIITVAGHKGGIGKSTVLCSLCVCVIRKGKTACFLETDSQGS
                     IKDFIEERKTNAQLSEIPYFECYTDIPAMVQKLAARFDYVFVDTPGMKSPAFVKALSC
                     ADILFTFVEPGSGIEINTLGRLVFDIKTAQAGVNPSMKAWIVLNKCSTNPSDSEASEL
                     RKQLNDDPDWLPVPRQRIYMRTAHKKAYNGGMGVHEYDDKRGNKARGEIELLLKETNI
                     I"
     gene            8638..8940
                     /locus_tag="ACH3L1_001444"
     CDS             8638..8940
                     /locus_tag="ACH3L1_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076241.1"
                     /translation="MAKLPKKIDQEIKPLDLRQFVSSAAQKPASASAVTRINLTMTDD
                     DLNTAQAFQNEYGASRAEVIRAALAALRLVSAEECRMLFDDIRKNSPKAGRPTVNK"
     gene            8976..9260
                     /locus_tag="ACH3L1_001445"
     CDS             8976..9260
                     /locus_tag="ACH3L1_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076242.1"
                     /translation="MLMTAIKSVFNFYMLCVQPLLGLFLVGYSIWSFSGDGICTADKV
                     IACDISIYLEPAFYLLIGTVVLFVWYIAWQTEHSKEEDFKSSIKPEDFVK"
     gene            9285..9566
                     /locus_tag="ACH3L1_001446"
     CDS             9285..9566
                     /locus_tag="ACH3L1_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076243.1"
                     /translation="MSEIYCIAIAVIIVGYVYLMISVITAPQKLFPMLQLITLIGISS
                     LFSFMSFKSESMWTGEPELLVAIMFGTFSATLIFYMFKILIRAFDSLTK"
     gene            9746..9970
                     /locus_tag="ACH3L1_001447"
     CDS             9746..9970
                     /locus_tag="ACH3L1_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFT4076244.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            9967..10353
                     /locus_tag="ACH3L1_001448"
     CDS             9967..10353
                     /locus_tag="ACH3L1_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFT4076245.1"
                     /translation="MKFVSSQEVIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMHH
                     YEGVTDIIDLAAVYLVAIARGHIFNDANKRTALFVAQVFLKRNGVHIISSRISFDEMQ
                     IIALNAATGEYNWKMVSDHLKAIILN"
     gene            complement(11899..12921)
                     /gene="repA"
                     /locus_tag="ACH3L1_001449"
     CDS             complement(11899..12921)
                     /gene="repA"
                     /locus_tag="ACH3L1_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFT4076246.1"
                     /translation="MASLNRPSLHWSELSPEEQILFWEDYESGRATSFLVEPEKRSTK
                     RRRGGHSTKPKCENPSWFRPDNYKALGGQLGHAYNRLVKKDPVTGQNTLRMHMSLHPF
                     YVLKRQSVGRKYKFRPEKQRLLDAIWVVLVSFCDRGLHTVGMSVSRLAEEISPKDSKG
                     DVIPETAVTVSRLSRLLAEQVSFGTLGTSEKTIWDRESRQRLPKYVWITETGWKMLGV
                     DLVKLQEQQRKRLAESEIRLQLIKEGVIREGEEISVHSARKRWYAQRSLDAIKSRRKK
                     AAERKRANRLAKLPYDEQRNEIARFILKRMPPDEAYWCTQERLEQLVARDLRQLELAL
                     TASPPH"
     gene            14064..14348
                     /locus_tag="ACH3L1_001450"
     CDS             14064..14348
                     /locus_tag="ACH3L1_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076247.1"
                     /translation="MSQAKANLKKLRHHCYIPPKQKISRLYYVEKALSSPWIYRERPA
                     CRAELLLTHDTLLVIYGLAKSGLIDRSEESLNREIKGVRKALAAGRFRWS"
     gene            14604..15092
                     /locus_tag="ACH3L1_001451"
     CDS             14604..15092
                     /locus_tag="ACH3L1_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076248.1"
                     /translation="MKHKNFIQKWYDSTFESILHDVNTIHSIIDEHHKLWKIQRLIRL
                     VILLIITAFGLFSAFLNRENIFYIFTVGFSLGLILYIIAAIDSICHFLPNPLSDHVII
                     DRETIQFLIVLTSHYPDVQQELFNRLLSGKKLTIHDEREIIDICSRRSATYHLTRSNI
                     PK"
     gene            15171..15752
                     /locus_tag="ACH3L1_001452"
     CDS             15171..15752
                     /locus_tag="ACH3L1_001452"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFT4076249.1"
                     /translation="MTLISQCAPGVSPETMRAIIMTESGGNPYAIANVTDGGSKYFTT
                     EEEAVNHAKKLTTDKKNFSAGLGQINSRNFQTLNLTHESVFSPCANIRAAAAVLKTCW
                     DRYETKGGDQQKILRDAMSCYYSGNNKRGYVKESNGKSYIDIIEEKAGIHTHYDIPAI
                     KSYSETSSHLMVSSDSHDKWDVFGDFRTQDFKE"
     gene            15831..16136
                     /locus_tag="ACH3L1_001453"
     CDS             15831..16136
                     /locus_tag="ACH3L1_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076250.1"
                     /translation="MHKSINLYRMFFLFVCAFFIASNPALAAGVGGLNKGTNFIGELR
                     LWAYGFVGGVALLHILWKVFQAYTEMDSWAGVLKSLMYAAIAGGCVVAAEACYQIFK"
     gene            16191..16514
                     /locus_tag="ACH3L1_001454"
     CDS             16191..16514
                     /locus_tag="ACH3L1_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFT4076251.1"
                     /translation="MDKEEIKAAEELKAIFLTYNGLNRPAMIKGMPIIPFILCLLALM
                     VSFFVGILTIDFYGLIIPSIIIGVMIAIKQMCADDPNAMSARALKFKGLCLKGFRSNN
                     VLKVE"
     gene            16516..18945
                     /locus_tag="ACH3L1_001455"
     CDS             16516..18945
                     /locus_tag="ACH3L1_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFT4076252.1"
                     /translation="MSIKKITNNFIPPYHSHIHENIIWLEDKKLLLTFLIEGIPYESL
                     TDDMILNNFNGQKETLLGLCKSERVFLWKHLVKRESPMDARYKFPGNPFLQRLGDFYC
                     DTFNGERSFRTDYFLTIGIPYDDIDAGEKKAKDIIRQIETGFKDYNIYTLGISDGGIS
                     DICSNYLNYLLNHDFEKIPLSATKISESIKNSKFYFSFDTLEIKNRTGHDNKFCSNYI
                     VKDVPRGSQIGQWNFLLSLPFEFILTQSFIPDSLSKSSKKIEQQLNKLTSVKDAGKTQ
                     HTELHMAQEAIINQETVFGSWHSVLSVFGNTPDEASDNGSEVAAEFVTSGRGFRFVRA
                     ASEAPYAWFSHLPASKLRPLATQRTLTNVCCLMPLHNHSYGKKSGNPIGDGSAIMPLK
                     TVTDGLYWFNTHYSAPGRNVTGQMIAGHGMLLGATGAGKTTFEMMAAAFIQRFNPDMF
                     VIDFNRSTELAIRMFGGQYFSLSEGVYSGLNPFQIGDNDDPELMAFLKEWVKRCAVNN
                     DGSDCSDSEAEEIDRAVEMVMKLERRQRRFARLQSRIQSPELNVRLSKWWDNGALAWA
                     TDSEENKFDPNQYSKIGFDTTVILETVNQRDHPACEVILSVLFFYKKRMQKGGKLMLS
                     IVEEFWKPCNYPMTQALIKASLKAGRMKGEMMWLTSQSPEDAINCQIFAALVQQTPTK
                     ILLPNPDAKYSGYKEIGLTEKEFRQLKKLKLESRTMLIKQSGSSVFAKMDLVGFDDFL
                     PVISCSMKGIKLCEKIREEIGSDDPELWYPPFIEGIYQIDDVIREANSDIPEVWLPFM
                     MKRLKDKLPEK"
     gene            19168..19836
                     /locus_tag="ACH3L1_001456"
     CDS             19168..19836
                     /locus_tag="ACH3L1_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFT4076253.1"
                     /translation="MKKTLTALFLGATLTGYSSVASSVGGVIVKDPGSIAKTVEVIKK
                     AADQIAELKKQVDLATSQLEAYKQEVLDTKRRLEGITDYSAIFGSAESYMKDFWEDMK
                     KELTDADIRSMATKYGFDTKEYDRIKRQYESKFEEITKYEAMSKDLEKSAEKIKATQK
                     AFSKADTPQKREELQNNILLETAAMQLKIAQNEAEINRMARERKLREEAALIKYTEDN
                     FSFN"
     gene            19929..20276
                     /locus_tag="ACH3L1_001457"
     CDS             19929..20276
                     /locus_tag="ACH3L1_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076254.1"
                     /translation="MFKKLLGGFILAFAFLLSGCDGDNGELAGTYIEKNPDSGDAPQK
                     WIIKYDKKDNYYTQIWIEKDKKGNEHNGTTNSFLIRDGDWLAGKEKHFIRVIDKNTLQ
                     LRDDENALYIRTN"
     gene            20342..21388
                     /locus_tag="ACH3L1_001458"
     CDS             20342..21388
                     /locus_tag="ACH3L1_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFT4076255.1"
                     /translation="MASNDSAFAKANFEIVQPMFNELSSIIQNSVTTNFQTIMQLVTP
                     LFGAAVILYGVFFAWEIMYTRKDDVMMESVKRMGVLTLVFAMSTAGGMYLSYIVPFVQ
                     GSGQEVASRLIGQDGASTGAVIDGLLNRLIDLGHEQFKVFQKADGWFDSMGAWIVFIL
                     KAILLALAGGGFVIYCAAYLLMAEMMVGILLSIGGVFILFSAWPTTRNMFTAWVGSCF
                     NYIFLNIAYACLFKVLIQYINNYINTNSDDITKNIWSVLCVAVVFLIGKFLLAQISTL
                     ISTLTGGVGINGLTSATGDVIKSLRGAGNSTRNQLGLGFNKTAQDARARNSEYAAAAK
                     DYLSSRFGGNIKAG"
     gene            21589..22335
                     /locus_tag="ACH3L1_001459"
     CDS             21589..22335
                     /locus_tag="ACH3L1_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFT4076256.1"
                     /translation="MGLKEYFKRSKAEARKTPAQKETDKKIEKNVLDAAKEFARDRDN
                     ELKRSLRITRFALFFSSASAAAMAVALAVMAPLKTTEPFLLRVDKTTGYTDVITPFSQ
                     DSGTYDEAVSRYFLTRYLENREGYEWYTIQNMLNTVELMSSSQVFAEYNNMIHGPLSP
                     LYKLKKSYKILVRVQSITFLDENTVQGRFVKAITDPDGKPAPGYTPTQWIATIKFVWL
                     KDKIATEAERQINPFGLDVLSYRVDPEVVK"
     gene            22332..23129
                     /locus_tag="ACH3L1_001460"
     CDS             22332..23129
                     /locus_tag="ACH3L1_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFT4076257.1"
                     /translation="MMMRKLLLSLVISGITMTCHAAKTPASAGFDRRVQQIVYNPNDV
                     TIVKTKAGEVTLIELEEGEIIKSEDTGLAIGDPEAWDPAVRGNTIFLRPKAAEPDTNI
                     AIVTNKRIYSIQLVSTDQNPTYLLRYIYPKPPEPVKPNVMTGRSRVVAPCTEGNIVNG
                     RYEVRGSRKIKPEAIWDNGRLTCFRWTGAADLPVVFRILPDGKEQLVNYHMDKNIMVV
                     HEISPAFVLRLGGEVMEVRTNRHIPRAWNASGTLTGETRVEINNDEK"
     gene            23119..24342
                     /locus_tag="ACH3L1_001461"
     CDS             23119..24342
                     /locus_tag="ACH3L1_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFT4076258.1"
                     /translation="MKNNNDIVIEKLDKIDTQEADTDAQNRNFIHSGKKQGNKKAKAV
                     LILAGFAFVTVVILTAVLSKVFSSSGEEETTETATTKAQAEVYAQNTQAKNLDDLKES
                     VARSIEEVKNNVNSEEEQAREEAPPENKENKGVTLPAENEADTGYTQSNGDAPPTPEQ
                     RRLMGSVMVDDSRITQSQPGSSGQKTAGATPASDNTNVSGDGDFLQGADFADGSVSRI
                     KNRRFLLSAGTTFPCVLKTMLVTTYPGVTLCQLTKDVISDDGKTVLIRAGAQLQGEQT
                     KAITQGMARVFVNWTMVKDRNVRVRINALGADSLGASGLPAWVDTHFWERFGGALMLS
                     LIEDTLAAGTSRLSRENNSDFTMNNTTGTSSELAKTTLDNSINIPPTGYVNQGEMLTV
                     IVPRNIDFSSVYKVR"
     gene            24342..25355
                     /gene="virB11"
                     /locus_tag="ACH3L1_001462"
     CDS             24342..25355
                     /gene="virB11"
                     /locus_tag="ACH3L1_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFT4076259.1"
                     /translation="METINMFLESTGVRPFLQAEGLTEIIVNRPFEIITEGVNGWEYH
                     EAPGASYSDLLDLSIAINKYNNSAEPLDALHPIKSLVMPGGERMQIIMPPACEERCIS
                     FTIRKPSLRRFTLEDYVSSNRFSNVRIVNQANLSTLSASQQKLLDLLDNTRCAPRTWI
                     SFLEAAVAARMNILVVGGTGSGKTTIAKTIADLFPRDRRIITIEDVHEMTLPNHRNHV
                     HLFYKQGGVQPRYLIEAAMRMKPDHIFLAELRGDEAWSYLEALNTGHEGSVTTIHANS
                     ALDAISRLASIVKQSTVGLTLDHSFILKTIKTSIDIVVFFRHTHMTEIYYNPMEKNQL
                     LAA"
     gene            25675..27708
                     /locus_tag="ACH3L1_001463"
     CDS             25675..27708
                     /locus_tag="ACH3L1_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFT4076260.1"
                     /translation="MKKKKLVLPVLIVWLMALTASGWLGSFILVKIASLPIDNLQWST
                     YLSTLAYYGWQPSLKKYLFAGLAGTLLPFLIALILTVVMFVAVREKKTVHGNARLAND
                     RDLADSGFFPDEKELKNQKYPSLLIGKMPENKGRFKSRYLQYSGQQFLMLYAPTRSGK
                     GVSIVMPNGIWYTESMVFLDIKLENFLETAGYRKNVLKQEVYLFSPDGYAREIGGETV
                     LTTHRYNPLYYIRRDPIYRFGDLEKIAVILFPMTGGENDTWVESSKNVFISLVLWLLD
                     TELEGKFKVNMNSVMKLSIPADGSGLAKWMLNEIRSRGNEVLTERWKTYHALPEEEKQ
                     GKAPSTYPLSDDTIRLFRQFAAKEPKQQESIMLDFNRTMSMFANPVVAAATEDNDFDM
                     RDVRRKKMTIYYGISPGALKQYQRLTNLFFSQLLNENVRTLPEHDATLKYQCTLMLDE
                     FTSMGRLDVVQVSLAFTAGYNLRFVFILQNREQLFDEKHGYGKMGGNTILKNCAVEIF
                     YPPKKVDESVKDVSETIGYYDFVRTQISRTTGKNPSTTRNKVIEKRAVMLPQEITDLR
                     DQKFKVKGKKTSFSLPQIVMSEFCRPFIAHKIVSFDQKNESFFVNARKYSRKNIIDTP
                     VLDIPQNEVVRQLMLQREREEAWKATQPAFDKDDFDDDKEYFPGDDDGGGGGD"
     gene            27948..28277
                     /locus_tag="ACH3L1_001464"
     CDS             27948..28277
                     /locus_tag="ACH3L1_001464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076261.1"
                     /translation="MKSRLLISITLLSALTFTLSASAKIKGLSAAGDPCEVLVCMAEK
                     VQGAPQPGCISVNQRFFNIRVYTPFYNPGATYGARQNFLYTCSGSTVNQATLNMIMQR
                     YGRLFSG"
     gene            28354..28692
                     /locus_tag="ACH3L1_001465"
     CDS             28354..28692
                     /locus_tag="ACH3L1_001465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076262.1"
                     /translation="MSFIKINRQREFMERSNSPRLVLRKEKRRNGIVNCYLSKTVRGD
                     DTFITIELNPEHNKIRLKTTNSPEGHKMSRGCFSLTKVFEYIFHENLHEDRIIIELEK
                     KENGWWYGNY"
     gene            28679..28936
                     /locus_tag="ACH3L1_001466"
     CDS             28679..28936
                     /locus_tag="ACH3L1_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076263.1"
                     /translation="MEITKNRETRFVTLASDFNNIKEIYSDDELNRLLEAVKNCPTII
                     VLGCANKGKETPLMASCLRTTPERISVEEFNEIKAISSKQK"
     gene            29031..29108
                     /locus_tag="ACH3L1_001467"
                     /pseudo
     CDS             29031..29108
                     /locus_tag="ACH3L1_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="micrococcal nuclease"
     gene            29124..29528
                     /locus_tag="ACH3L1_001468"
     CDS             29124..29528
                     /locus_tag="ACH3L1_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFT4076264.1"
                     /translation="MALSPKEKKELQQRFEKQLLPDLDLSFELNGEVYKFNFDKNKSG
                     DYIDSFTQAVWGGYQDAEEWKKYGKHQETELPEYPEPVEGSCDCDKCKSMIFHKEKNI
                     YVCLSCHKQTEPFLPIDWNSNDYDWLPYEDEY"
     gene            29588..29896
                     /locus_tag="ACH3L1_001469"
     CDS             29588..29896
                     /locus_tag="ACH3L1_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076265.1"
                     /translation="MNIHLFSEVLFCVWVIALIVILFIVVKYYRRVHYRLNSLSETIK
                     RTQGGVNKRISENRELLELIKNQHPEILDEYPWVSGWLDSQEKFLVALADKSGIDRNS
                     "
     gene            29992..30378
                     /locus_tag="ACH3L1_001470"
     CDS             29992..30378
                     /locus_tag="ACH3L1_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076266.1"
                     /translation="MMTKYDKERFNKRISDEVQISADIRINDFMTESATYVIITESSL
                     YERICQYALQHGEDLQGMFKDERYEYMSCFVRDVAAFRANFESEEMLKPLFNHGKGDT
                     VEFVISVPEKRVKDYGGAESVETKKR"
     gene            30394..30696
                     /locus_tag="ACH3L1_001471"
     CDS             30394..30696
                     /locus_tag="ACH3L1_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076267.1"
                     /translation="MYLELLIIKRRKLSPPFPEWKSGHYIEIPGEGIVFTDKKNDDGS
                     RCYSGVIESHYISDDWAFILSEYKNWLKDKERKFSYLFVLRNGELVHHEVNKSELL"
     gene            30723..31310
                     /locus_tag="ACH3L1_001472"
     CDS             30723..31310
                     /locus_tag="ACH3L1_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6012 family protein"
                     /protein_id="HFT4076268.1"
                     /translation="MYIHIVPKLYHLMANKCSLKSISIPEFDFIIDSESLSVGRPWPN
                     KCLWVGMRKGRKSVNGLVLQTDKKLRWFTTIYTWDIEDMGLIYHRVNTYIEDDDFDMV
                     SQEILLNGSFDKWSNRVHSAYENKPPARIQPKMESLLNKPGENSHDVWEEFEWGDFLL
                     SREESLLLHTIQSERLSTDCSLFKRQPSIESSLVI"
     gene            31322..33478
                     /locus_tag="ACH3L1_001473"
     CDS             31322..33478
                     /locus_tag="ACH3L1_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFT4076269.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHI
                     IESQPPENYNPDYKAWKVETLPLRLYPVKYQPVESAAKQVNTILELIRRGDVTEIVHA
                     GDPDEEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKAL
                     ARSVADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFY
                     YTMTGVFQRGADVIRANWKPGEFAPLTDRKLLEKAWADGTAASLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQVIDALKSVFPQSLDIDSSRKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGE
                     SEDNDDSAFEQLCKIRTGETLTTKEVVVNEKKTTPPPLFTEASLLAALVRVADFVTDP
                     VIKKLLKDKDKDKKDEHGGIGTPATRAAILEALKKRNYITLEKGKLIPTDTGCALIDA
                     LPGIAVNPDMTALWSEKQSAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAP
                     VGLSQRLDSPCPSCGKQIVIRPKGYFCTGCEFKIWKNFSGKALSDKQVETLLKKGITG
                     ELKGFISSRTNKEFSAKVKLMDKATGKLGFEFIPKK"
     gene            complement(34204..34299)
                     /gene="dqlB"
                     /locus_tag="ACH3L1_001474"
     CDS             complement(34204..34299)
                     /gene="dqlB"
                     /locus_tag="ACH3L1_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFT4076270.1"
                     /translation="MINLLIIVLRAVVAVANALIAVLELIRQFID"
     gene            complement(34666..37953)
                     /locus_tag="ACH3L1_001475"
     CDS             complement(34666..37953)
                     /locus_tag="ACH3L1_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFT4076271.1"
                     /translation="MLVRINGGNKGVCEYLEKGYKQGREYTRDEIDHRLVLDGDLALT
                     ESVIDTIPDNGQERYIHITLSFFEDNVSESKLHDVVQEYKSLMMNAYHEDEYNFYAEA
                     HLPKIKQMPDYKTGEMVERKPHIHIVIPEVNLVTGSKLNPRGMTEHYIQYLDAIQEHI
                     NNKYNLVSPKDGVRVSDHNHANVLSRAKGDFYRERHSDFKTTVFDALDTENIRTEKQF
                     ADYLSRFGEVKEYNKGESNRYFGVKLEGDSKFTRLKSPLFSRQYISDRSIPLLKPTKT
                     QIESRLKEWKSSVSHEIKHIHPAGQKIRKEYAALAGEDKQMYLKKVINRYDEKHNLQR
                     QDRRQNSDQSGIKYNTGKFVSGKTIGLPRLPARELVYGLHGRSGGKSINKSQLLLQQN
                     EYGYLSTAGRQKVDPDRYVRRLQDRAGRRVVQSALESNIKAAIQRSDDLPFLKQIRTE
                     INAERYLSNLQTRFNIDPSKHTVTYGQDGSPRFRVGNRNMNASDFLTKHLNLEWDEAK
                     EVLLDIWQDQKDNKPFDAVITRVNLTRQQAKERFICLNEHKGWVNNEIKYKHNDNIAN
                     YRAELRGLLAIRNKQEREVARGYIIFKKLEREREINSIRKACFREINSYYHRWNPDRR
                     YDMALGDSLKKIIRNPVDGNSISSGNEKEVFSVVKRVQQSQQVQDYVKRELTDLVPHK
                     VSPKETRYLDPKTTQTVFTDKGNHVRVNGEITQDKAEAALLYAKEKFGGMLKLNGSEE
                     FKVACAMAAAEKGMNVILRPEKYHEMMQVRLQELKAEHGHENSVEAERQQQVAGQKPE
                     QEQEKRAEQVNSVEKDTQAKERTPEVAESVRNDDKSRDAVPEQPVKSETVSQKSTSET
                     SVVQEAEKQESVQQNVSAHSEEREQQPQEAQQQKPVTEKDVTTGQDKQTGDEQSKSAL
                     ATENPHQQNNASLQQLQEEAKVIASQHQEAQRLLNEAVENGAPKEEVDLLSKGVNSIS
                     ARAQTINEQIKAFEEQQGQKKAKSVEEKNENTTEKKKRTVNSEKAMEREIERISKQLA
                     KEMNIDKQTVKDTLKFIRFEDVNDLKAAMKNPETVEAVKREIVSYDNALKNERERERN
                     RDNTRSR"
     gene            complement(37947..38333)
                     /locus_tag="ACH3L1_001476"
     CDS             complement(37947..38333)
                     /locus_tag="ACH3L1_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HFT4076272.1"
                     /translation="MTKQDKGKKAGASTSNVERKHVISLRLTDEEYQSFHQYLTSLDV
                     SRSEFFRMLILNRLDDIPALKPKRPTDYRELLRLFNKSSNNINQIAKKINTEYRNGFI
                     SESTYLRYLNVLVNIRDAFVTGVDKC"
     gene            38694..>38961
                     /locus_tag="ACH3L1_001477"
     CDS             38694..>38961
                     /locus_tag="ACH3L1_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HFT4076273.1"
                     /translation="MKKIDSLLSPICEAIKETNSKSKQAVFNMYLSNILEAKSYYTWE
                     QITDYINNKTKTELAQKTYINMTERAKKRQKCTGQKDLKKSTNMI"
BASE COUNT        11406 a         8489 c         8233 g        10833 t
ORIGIN      
        1 aaaaaaaaat agatgaagac aacaaacaaa gagggggcat aaaaggattt ttttcaacaa
       61 tcagcgagaa caaaaacgaa atagatcacg atccaagcgc aacaaatgaa aaatttataa
      121 acaaatatct ttaaataaag gtgtataaca aatgaaaaat tcaataaatt ttatccttca
      181 aggaaaaggc ggtgtaggaa aatcatttac aacggcaata ctagcacaat actttattga
      241 tgaaaaaaac atggataacg tcgttgtagg cgacacagat cctgtaaata caactacagc
      301 taaagtaaaa agattaaatg ctgatttaat acaaatcact gaaaacagca aagtagtcca
      361 atctaaattc gatccaatgt tcgaatcaat gctaactaat actcaaaata catttgtcat
      421 tgataatggt gcatctactt ttttaccatt aattcagtat ttcaatgaca actgtgtaat
      481 ggatatgttt aacgaagttg aacaagaagt ttatatccat actattattg ttggcggtca
      541 ggcgcaagca gatacaatag aaggttttga agatcttgtt aaactagtaa aaggaacaaa
      601 agtaaaaata atagtatgga ttaatgaatt tcagggtgta ccagcacttg aaaacattcc
      661 tttaattaaa actaaattca ttgaaaaaaa taaggatgtc atcgctggag ttgtggttat
      721 tcaggacaga aaaagcgatg cctttgcttc tgacatcaaa aatttaaccg aaaaatcact
      781 aacattaaaa gaagcccttg aatcagaatt ttttggatta atggctaaat ctcgtctcaa
      841 gcgagttttt aacgatatat atgtgcaact tgattcaatc tataacatgc agcacacaga
      901 agcataagta attatataac agccattatg ggctgttata tatatgagga caagttatga
      961 ttaatgataa atcatttaat atcgaaaaca ttatttctga tatttttaaa gagacccgat
     1021 taaaaataag taaggacgat cctgttttaa gcataatact aatgcatgaa aaaattctcg
     1081 aacatgcact aacacaactt aaaaacaata atcaaatagc tactgaaaga ttatctcatg
     1141 acatatcttc aataagagat gcaatcaacg cattacctga cgctattgat gaaaaaacgt
     1201 cagaactaca acatgcagca gtagcacttc atgacgaatt tcaggaaagc aaaggagaaa
     1261 ttaaaggttc tctggaagaa gctcgaatta atgcaacaga gaaattagca gaaagtgcaa
     1321 aagagcttca attaaacatt acaaaagttg ctgaaaaaac aacagaaaca atagagtcag
     1381 caaataaaat aatatctgcc atagatacaa acctggctga aattaataaa aaagccttag
     1441 ctaattatgt taatgatatc agaagccttg aaaagaaagg tgaaagtata tctaagaata
     1501 ttgatacagc aattaataat gcttttaaat ccagcgtgaa atcatttaaa ttttattgtg
     1561 gtgctgcatt atttattagc actgtcttac aattcacaat gtggggattt tttctctata
     1621 aactcctgac ttaatcttaa ataccgcttc cagcggcata tttggggtca gtttggatat
     1681 agagaattat tgtacgttca gcacaacaaa cttaatgcta atgtatgcat acattagcat
     1741 taaccacaca tttaagactt aacacagtat taatgtgtat tattacacat taatatacat
     1801 caggaagcac gtcgtcagta ttggagaaaa acttacaaac gctgaccaca gccccagcga
     1861 ccgaacggta gtgagcgagt gggcgaggaa gaagaagacg gccagatctg aatagcgctt
     1921 gtgagaaggg gctacaagcc ccttcccgta tttcatggta aatcaccgca accgagaggg
     1981 cagcaatcgc gatcaaagcc gtgacaacaa tagccacgtt ctgagtcacg gcgtatttct
     2041 tcagcaatct cttccgcaga aagataatca aattcctggc gagccagttc aaacgcctgg
     2101 gcgtgttgtt cttccggcaa tccggcgaca actttatcaa tcgcagcact caaacgacgt
     2161 tggtatagct caaaaacaag accacaacca ggcatgatct cttttgataa aacatccagc
     2221 tctgagagaa atttttcact atcgaaagta tccatcatct tcaccttcat aagttaccag
     2281 atactatttc tgcatcagtt aataacaggg gccataagcc cctgttatat ttatttccct
     2341 tctatagcga ttcttctaag ctcatgaaga atacaagccg tttgatctac cagtttttcg
     2401 tcatgaatga tgttttctgc tattaactcg ttaaactcta cgaccagaaa atatgactct
     2461 ctgtaaacgt catcctgatc aaattctgcc gctctaaact ttgaatagat gataatgccg
     2521 cgatcttgaa ttttctgaat ttgcttaatc acttctgaac tcatttttaa tctcctgccc
     2581 gtttggactt aagaaggaaa ggagccaatc tcctttcgat gaaataataa tacatccatt
     2641 tagatgtatt ggcaaggttt ctggcctgaa aatcggtaca ttatttgatg attgtcacgc
     2701 tttttatccg cgccagcgga tgtaaggggc gaacgaaaat accagggaag cacaggagcc
     2761 acatcgcccc gaagcgcggc aacgccgcta ctgttatcaa ccggaacctg ggccaccttc
     2821 ccgccctgct aagcccgact gactcacaga caacgatgac gctgatttgc gccgcagaat
     2881 gccgcagtta gcgctcgcgc cagcgagttt acgccgtcca gggtttccct ggacggggcg
     2941 ctgtactgcg cgtgtgagcg ccagcgagct acgggtgcaa cacaccagaa gttaaaccgc
     3001 tcttaatatg gtttgcatta aaatttttct tgccgaatcc tcattaatat cacacttgaa
     3061 ggaaattatt tttattgctc tctttttcag agcacttttt atttgatctg ttgataataa
     3121 atccctgtaa atcggatgaa gataaatcgc atgtttatct tcaaattctc tgtcaatttt
     3181 tgatgcatta ataatagcat caaaacaatt atcatcatcc acaggacaag ctgtccaaaa
     3241 catcatttcc acagcaattt ttttagcttc attaatcatc ccctgccccc cctttattac
     3301 ttgcagacga ctatagttac atatagcatt tttgtctaaa tatatgaagg ggcagaaatg
     3361 ccccttttta tttaaaacat ttcataccct tgcaggtagt ttaaccacca ccggaattga
     3421 tcccactcac taaacgtcat tgccgggatt ctggtttccc agcctttgac aagagaatca
     3481 gcaagctgta cgatctgtcg tgttgacatt taaaaacctc attcagtttt gattgtcgat
     3541 aacgtgttca ggttgttcca gcaacctgaa cacacctttc ataatttatt tcccccttat
     3601 acatccgata tattctttaa aactcaaata ctcctcgcca ttacttaacg agtcaaaata
     3661 tttttcatat tcgtcccgat agaaaagaat taaatcaata tcactctgct taagttcacc
     3721 aaactgagaa acaattccgg tcttatagtc acgaatttta ttgttatccg taaatatgac
     3781 aacatactgt tgtcccgtat ccttatgaat aagaacaatc gtgcaaagcg ttactcgttc
     3841 gaatgtgaaa acatgattcg tggttgaagt gaatttcatt ttaaatctcc tgtccgtttg
     3901 gacttaagaa ggaaaggagc caatcccctt tcgatgaaat aataatacat ctatttagat
     3961 gtattatcaa gatttattgc ctgaaaacag caagttattt gatggcttgc tcatttttta
     4021 atccgcgaca gcggatagaa ggggcgaaaa aaaataccag ggagcagcgg ggccacatcg
     4081 ccccgaagcg cggcgcagcc gctgacgaac ataccaggat gcacagggcc atcccttcgc
     4141 cctgcaaagc cagaacagac caacaggcta tgatcatgct gatttgcgcc gcagaatgcc
     4201 gcagttagcg cgagcgccag cgagttcacg ccgtcctggt gcattgcagc agggcggggc
     4261 gctgtactgc gcgtgtgagc gtcagcgagc tacgggcgca acaacacacc aggcaaaatc
     4321 gcaattatgt aaagtcgccc tgttacaaac acacactgtg taaagacaaa taaaccttta
     4381 cactgtatat cttagatttc tgcgtcgtgg tgtttttctt ctcgctcacg gttcctccgg
     4441 tgagcgggat tcagcttttt tatgcaggac gtattcaggg tgctcccctg caagtaataa
     4501 caggtactca aattcacctt tcgataaatt aagctggttg tgggttttag cttctttttt
     4561 ctgccatgta cttaagccta cgccaaaaat ctcaccgcac tctgtctggg taaggccaat
     4621 ttttttacgt aatttcttta tgttttccgg tgtattttcg atagtcatgc atcactctcc
     4681 ttatattctt tcaccatagt taagagaata ttcttatcaa caggtcttaa accatttttt
     4741 gtaaagtcta ttgcctcgac ctcactttca ttaaaaacca gctcacttaa cgttctcttt
     4801 gttctttttg tttttccgtc agtgaactcc tttgaataac tgcttatttt ttttacaaaa
     4861 tggtattttt tatcaatcac ttcaagataa taagtcaatc gccctttgtt ttttctcata
     4921 actgtttcaa atatcctgta attttaacaa gttgatctat actcaaacct ctaatctgca
     4981 aatcataaca gtcgccctgt tcggtaactt taccgttcaa tccgttttca gctttaaagc
     5041 ctgtcaatgc cccataccag ctttcaagct ggacaagttg cgataacata cctttcagga
     5101 tcagaatttc gtcctttttt gctgcctggc gtttctcata ctcaatcgcc tgtaacacct
     5161 cctgcgaggc atttaccaga atttttcgct cgatatcggc tgctatatca atgggattcc
     5221 gcttcttgga taacgtgcaa acatgatacg gactacgcca ggaacgacta tccacactcc
     5281 ctacaacaga aaatcggtct ttctccatcc gaatatgcac ggagtaattt ttaaactgcg
     5341 gtgacgttag ttttatacga gacgaacaat catcaagttt gttcacgcgc cagccgttcc
     5401 ccaggatacg gcagacaact tcaaagacag ggtgatattt ttcaaaaaaa gagttcatct
     5461 gtttcccttt ttgctatgtg gcggctgttc ccgtcttttt ggagcctcaa aagacgggct
     5521 ggctgtgata tcgggggcat ctcgcccccg ttctggttac gctctggttt ccacgcgttg
     5581 ccaccctgtc gccttgcgct gtccggtcgt accgtacttt gcgcgaattt cttctatcgt
     5641 gtgttcttca cgattccagt aacttttatg ctcatccggg cgataaaacc attgtttctt
     5701 ttttggtgcc catttacagc cgatttcttt caaagtttcc ttatgcgtaa tggtttcgcc
     5761 actaatccag acccaattac ctatgacttc aaataccaga ccagacaaac cagaaagaac
     5821 gtttaatact ttttccaagt cgtcaccgta gttatatatt gcactttttt cggtgctctg
     5881 gtactgatta attttgtcca gattcgccat caaaaaatca aaagctgcat ttaccgcttt
     5941 catcagttca gcgcccaacg ggttgcgatc gggatgatat tttaaagcgg cttttttgta
     6001 tgcggcttta atatcttttt ccgttaactc gccggataaa ccaaaaacat ttaacgcttc
     6061 ctggatattc atttcaaatc tcctgtccgt ttggacttaa gaaagaaagg agccaatccc
     6121 ctttcgacga aacaataata catctattta gatgtattat caagattttt tgcatgatat
     6181 atgcaaatta tttgaggaat tagcgtgttt tttaatccgc gtcagcggat agaaggggcg
     6241 aacgaaaatt ccagggtagc gcaggagcca catcgccccg gggcgcggct acgccgctac
     6301 tgttatcaac cggaaccagg gataccctcc cgccctgcta agcccgactg actcacagac
     6361 aacgatgacg ttgatttgcg ccgcagaatg ccgcagttag cgcgagcgcc agcgagttta
     6421 cgccgtcctg ggtttccctg gacggggcgc tgtactgcgc gtgtgagcgt tagcgagcta
     6481 cgggcgcaac acaccagaaa tcacatcacc ttaaaggatg tattaataca cacctagaat
     6541 ttctatgccg tacacaccta gaatttctat gccgtacaga ataaaaaggg gcgattaagc
     6601 ccctgcgcga tgattaaaag aggcgcgaac aatctgatga tttttgctaa cttgaggaaa
     6661 ttcccccgct tagggggaac tggagagatt agtggctgga tttactgaga gtgaattgta
     6721 atccggctac cgatgtaagt tgtgcaagtg ttgtgatggt cgggttcccg ttcggggata
     6781 atgcgcggta aatactctga cgggcaaggc cagatttcct ggcagcttcg gaaatccccc
     6841 ctcgcgcctc tattacctga cgtagcgcaa caagaaaacc gcctaaacca ccaggctcgt
     6901 ttatttcctc tagggcatta gcaagataag cgtttgcata atcaggatct gcgcgcaatt
     6961 cttcaatcat ggctgcattg tgatcaatgg atgccggata atcatttttc tgcgacttca
     7021 catttttggt ggctgtcatt tttttgacct ctttttaaaa tcttgcagat aactaacggc
     7081 tttatctaaa tcagctgtct gagttttttt attcccgcca atgagcaaaa gaattatttc
     7141 gccatcttcg atcgaataat aaaccctgta accaggacca taatcaactc gtaattccca
     7201 gacaccatcc ctttcaaatt tatgatctcc aaaatttccg cccatagcgc gggcaattct
     7261 ggaatccacc tttaacgctg cggtctggtc tttacgtctt aattttttca tccagtcggt
     7321 gtatggaacc tcaccatttt cggtctgata tcgtttcgct gtgtaaggca tcttgccgcc
     7381 ctcattccct taacctgtac acatcgtctc ttataagtga caccagttca agaaaaatgt
     7441 ctctttaaag tgacaaaatg tgcgtctagt gcggcagaac catatcgccc taaagcgcgg
     7501 cgcagccgct gacgaacgta ccagggcgca caggccatcc cttcgcaact gaccagaaag
     7561 ccatgattat gctgatttgt gccgtagata gcgcgatcgc ctctgtctgt acaaaataat
     7621 attacacatc aatacacacc ataaaattct tgccagcacg caatagtgca gatatactcc
     7681 acactcagct aattgtgtat taatacacac cattaatcgg gaggattttg tgatgggaaa
     7741 aatcattact gttgcagggc ataaaggcgg cataggaaaa agtactgtac tttgctcctt
     7801 atgtgtgtgt gtcatcagaa aagggaaaac agcctgtttc ctggaaacag attcgcaggg
     7861 aagcatcaag gatttcatcg aggaacgtaa aactaacgcc caactgtctg aaatacctta
     7921 ttttgaatgc tacacagata ttccggcgat ggtgcaaaaa ctggccgccc gttttgatta
     7981 tgtattcgtc gatacgccag gtatgaaatc cccggccttc gttaaggcac tgagttgtgc
     8041 tgatattttg ttcacatttg tggagccagg atctggcata gagatcaaca cactgggacg
     8101 actggttttc gacattaaaa ccgcacaggc cggagtcaat ccgtcaatga aagcatggat
     8161 tgtcctgaat aagtgctcaa ctaatccatc agacagtgaa gcgtcagaac tgcgcaaaca
     8221 acttaatgat gatcctgact ggcttcctgt tccccgtcag cgcatctata tgcgaactgc
     8281 gcataaaaaa gcctataacg gtggtatggg tgtacatgaa tacgatgata aacggggaaa
     8341 caaagcccgt ggagagattg aattactgtt aaaagaaacg aatatcattt aatgtgtatt
     8401 attacatatt aaataaaaag tattcccatc tccaaaatac tgtatacggg caattgggct
     8461 gcccggaaca ataatagtgt gtatttttac accatgaaac atgacgtgca caatcttttc
     8521 ataatgcagt catgtttctt cttgcaagtg ccctaacaat ttgtggtaca tggatgctgg
     8581 aacttttcca gccacaaaat gtgtgtataa ttacacactt aaaaaacagg ataccttatg
     8641 gcaaagctcc ccaagaaaat agatcaggaa attaagcctc tggatttgcg ccagtttgta
     8701 agttctgcgg cacaaaaacc ggcatcggca tccgcagtaa cgcgtataaa tctgacaatg
     8761 acagatgatg atctcaatac agcgcaggcg ttccagaacg agtacggtgc aagccgtgct
     8821 gaagttatca gggcggcact ggcagcgtta cgtctggtat cagcagaaga atgccggatg
     8881 ctgtttgatg atataaggaa aaacagtcct aaggccggaa gaccaaccgt taacaaatag
     8941 catgtattat tacatgtcgt taaaggagta aaaatatgct tatgacggcc attaaatctg
     9001 tattcaattt ttacatgctc tgtgtgcagc ctttacttgg attatttctc gtgggttaca
     9061 gtatatggag cttttcaggt gacggtatct gtacagcaga caaagttata gcctgtgata
     9121 tatcaatata tctggaacca gcattttacc ttctcattgg cactgttgtt ttgtttgtat
     9181 ggtatattgc atggcagaca gaacactcga aagaagaaga ttttaaatca agcattaagc
     9241 cggaagattt tgtgaagtga gattatataa gaggaaatgc cattatgagt gaaatatact
     9301 gcattgcaat tgcagtcatt atcgttggtt acgtttatct aatgatcagt gtaattactg
     9361 caccacaaaa attgtttcct atgcttcagc taataactct aataggtatc agttcgttat
     9421 tttcttttat gtcattcaag agcgaaagca tgtggacggg tgagcctgaa ttattagttg
     9481 cgattatgtt tggcactttt tcagcaacac tgatttttta catgtttaaa attttgattc
     9541 gcgcttttga ttcattgact aaataacaaa gctgaataat cccgctgaca aagcgggatt
     9601 atctttactc ttcattatat aggattccaa gccgcaaccg ccgttaaccg gacagcaaag
     9661 aaataatccc tactacggat ttgtgatttt atctttgacc agaagtgctg tacaatttaa
     9721 ttgtacaact caaagaggaa gcactatgtt tacatgtaac tatagccatg ctcgcgataa
     9781 cctggcctcg ttactcgagc tggcggttga cgaaaatcag cctgttgaga ttcagcgtcg
     9841 caaaggtggc gctgctgtac tggttgataa agacgctttt gaacgatacc gtaaagcggc
     9901 tcttgatgct gagtttgcag aaatcatgca aagtcatggg acggcctata cggagctttc
     9961 taagagatga aattcgttag ttcacaggaa gtgattgagt ttcatgaccg cttgatttcg
    10021 cgtgatggtg gcgtggcggg tatgccagaa cccggtcgcg ctgacgcgat cattcatcgt
    10081 gttttgaaca tgcatcatta tgaaggtgtg acagatatta tagatctggc tgccgtgtat
    10141 ttagttgcta ttgctcgcgg tcacattttc aacgatgcaa ataagcgaac agccttgttt
    10201 gttgcacagg tttttttaaa acggaatggt gtacatatta ttagttcacg tatttcgttt
    10261 gatgaaatgc aaattatcgc tttgaatgct gcaacaggcg aatataactg gaagatggtt
    10321 tctgaccatc ttaaagccat tattctaaat tagaatatgt gtaataatat tgatttaaca
    10381 tcaaaggcta tcgcatttag gttatccgat taaggccaaa gcccctgaaa atgccgtaga
    10441 ggaaagagga aaacaatcgt ctttatgaaa ttaattccga ggcaaacaga ggatggcgtg
    10501 atttcatcct aaaattgata ggggttaacc tgccaaataa aagtaccgag ttcacacaat
    10561 gtctcaatct ctgaagacag aactttacag tggcctggtg tggtctaagc attaggatcg
    10621 cctcgtggga taatgaaaat aaccacgagg ccttcttctg tttgccagcc attaaattcc
    10681 cagctttcgg cgtggactat acagtgtgtg attcaccacg ataaatcctt tgttgatcca
    10741 ctgtgttacc tgtgcgggtt gaactccggt tagcctggcg aacgatgcct gattcccccg
    10801 gtagaacttg ttgatgtatt caatcaaagg cattttttag tctcctaagc aatataaaaa
    10861 tcatatagcg ttttactata tgattatgtg cgtatgtgcg caattcagac caggtactct
    10921 ttcgcttcct cgcccactcg ctcactaacg ttcggtcgct ggggctgcgg cgagcgttac
    10981 tggctttttt agggcaattt tgagaactgt cagcctgata aacaacattg tgtaacaata
    11041 cacactaaat atgtcgcgcc agcggcatgt aaggcggcac tctgttgttt catatgatac
    11101 cggagtaaaa ccgccgaagc ccggcgtaag ccggtactga ttgatagatt tcaccttacc
    11161 catccccagc cctgccagac catacccgct ttcagtcatg acagagcttc tgtgcgcggt
    11221 cggagtggtc ccgacgaggg tttacccgaa gtcggggcgt atctccgcgt tagcgggccg
    11281 tcagggccgc ttacgagcgt gtacgcaata cttccagcca gaagactgac agcgatgaga
    11341 tttcttagtt acaacatcca ttattgaaag cgatgttgtt cccgcccttt gggcgggggc
    11401 ggcggcgctt ttcagtgatg ggttgggcgc tgtggctgag tatttgcagc gtttttttgt
    11461 aggagggctg gaggggcttt gtttttcatt aggcagaagt ccatatgccc gccgcgaagc
    11521 ggtgggaggt acttttatat tctgagcggc gcggcgttag ccgctgcaag cgaaggcatt
    11581 tttgtgatta tccacagttg tttcaaagat aaatattttc cctgcggcga agcaacgcag
    11641 gggattaaag aacaggggtt aagaatatat tgcgggtttg atgttctatg tttgctgctt
    11701 tctgaagtta tttctttgcg ggtttttgtg gataaccgaa ggcgttttta ctccgatacc
    11761 ctattgcgga catcaaaaaa ctgtcagagc gggacaaaat gaaaaacggt cgtcaaatat
    11821 gaggaatttc atgaaatgca ggcaacacga aaagccccga aggggctgaa gagagtgccg
    11881 atgtatcaga cagctaaact agtggggtgg cgaagctgtc agggccagtt caagctgacg
    11941 tagatccctg gctaccagtt gctctaatct ttcttgagta caccagtatg cctcatcagg
    12001 tggcatacgt ttcagaataa accgcgcaat ttcgtttcgc tgttcatcat acggcagttt
    12061 tgccaggcga ttagcacgtt tgcgctccgc tgctttctta cgtcgggact tgatggcatc
    12121 gagagaacgc tgcgcatacc agcgtttacg agccgagtga acagagattt cttcgccctc
    12181 gcggataacg ccttctttaa tcagttgcag acggatttca ctttctgcaa ggcgtttacg
    12241 ttgctgttcc tgaagtttca ccagatcaac tcccagcatt ttccagccag tttcggtgat
    12301 ccagacgtat ttcggcagtc gctggcggga ttcacggtcc catatcgtct tttctgacgt
    12361 tcccaacgtg ccaaaggaaa cctgctctgc cagcagacgg gaaaggcgcg atactgtgac
    12421 tgcggtttca ggaatcacgt ctcccttact gtcttttggg cttatttctt ctgccaggcg
    12481 ggagacagac atgccgacag tgtgcaatcc acgatcacaa aaactcacaa gtacaaccca
    12541 tattgcgtca agcagccgct gtttctccgg tcggaattta tatttgcgac cgacactctg
    12601 acgtttcagt acataaaatg gatgcaacga catgtgcatt ctgagtgtgt tctgaccagt
    12661 gaccgggtct tttttaacga gacgattgta agcatggccc aactgtccgc ccagcgcctt
    12721 atagttgtcg ggacggaacc aggacggatt ttcacacttg ggtttagtgg agtgcccacc
    12781 acggcggcgc ttcgtgctcc gtttttccgg ttcaaccaga aatgacgtgg ccctgcccga
    12841 ttcgtaatcc tcccagaaaa ggatttgctc ttcgggagac agttcactcc agtgtaatga
    12901 aggtctgttc agggaagcca taagcaatcg cttatggcag agcttgattc gcacagtgca
    12961 tttttacacc ggacgatatt gaataatgag gaaagcatca tgtatactgt cttccaaggg
    13021 atttcccctg cccccattaa actttgatcg cctctgccaa gaaacggatg caaagttaat
    13081 gggggtttgt tttttatggc ttcatggaat ctttcaactc ctgccataca cctgaatgcc
    13141 ttatggcgca tcctaccttt atcccctatg acggggacca aatttctcaa atttaagcga
    13201 agatcaaaaa ggatcaacac cgaatgccgc ttaatatacc tgtagccctc cgatttgaaa
    13261 agtgtttgtc gtgacgctca ttccaccaat cccgccaaat cagcacagcc agcactgccg
    13321 atgaaacaat caaaaagtat tttttttcgc cacttcttta atttttagaa tattagatcg
    13381 tgaatgatct attcaacgat ctatcatacc aatataaaag ctgattttag aattttatgc
    13441 tttttatttt gctttatgtg ggataataga tcgcccaaac caccttgaac gatctattgt
    13501 actggtttaa agtctccatg ttgaagtttt ccgctatcag ccgaacgccc gaacaaggca
    13561 ttattcgtac gctctttaac acccctggac agcggtaatt gcctgaagcc ctggcatgag
    13621 gaggattaat tgtgtgcaaa aaacagcact tgctgacaat gttcagtgtg ggctacgtat
    13681 agagtgacgt gccgcgccat ctgacaggta acgactcatt tttacaattt tcaacatagt
    13741 taattccctt tatcaaactc gattccctga aagggggatt aactgtgtac gaaaagtaca
    13801 ggagataata atgagagatg ataattatct tgatggatta attgcctgag gtactgttgc
    13861 aatttctttt gcgggactga ttctgctaat cgtgagtaat ttgaaaaacg aagctgagac
    13921 agaaaaaact gcattttatg aatgggccga ttcacactgt gaaaaaattg aaaaaaattt
    13981 aaatgttact gaaaattcga aaacatttaa gtgtgatgac ggaaaaattt acacaataaa
    14041 atggtgaaac agattaacat gttatgtcac aagcaaaagc aaacctgaag aaattacgtc
    14101 atcactgtta tattccacca aaacaaaaaa tatccagact gtattatgtt gaaaaagcct
    14161 taagttctcc ctggatatat cgtgaacggc ctgcatgcag agcagagttg ctacttacac
    14221 atgacacact gcttgttatt tatggcctgg caaaatccgg cctgattgac cgttctgagg
    14281 aatcactcaa cagagaaatc aaaggggtcc gaaaagcgct cgcagccgga cgtttccgtt
    14341 ggtcgtgaga tgccgcgcca gcggcatgta aggcggcact ctgtacttaa atatggcttt
    14401 gcgggttatc cgccgaagcc ccgcgccagc gggtactgtc ttctatattc tctggaactg
    14461 cgctcgcgcc ccctgctaag cccaaaacgc acggaaccaa cagctacacg gttgtttctg
    14521 gtcgaagacc gacactgctg aagcgatatt attcgaaagt tatcctactg tgcaatgccc
    14581 ctgttttatt tgaggaaaat ggcatgaagc ataaaaattt tattcagaaa tggtatgatt
    14641 caacttttga gtcaattctc catgatgtta acacgattca cagtatcatt gatgaacacc
    14701 acaaattatg gaagatccag agattaatac gtcttgtgat tctattaatc attacagcat
    14761 tcggtttatt tagcgctttc cttaatagag aaaatatctt ttatatattt acagtcggtt
    14821 tttctttggg tttaattctc tatataatcg ctgctattga ttctatctgt cattttctcc
    14881 ccaatccttt aagtgatcac gtaataatag accgcgaaac aatacaattt ctgattgttc
    14941 ttaccagcca ttatccagat gtgcaacagg aactatttaa cagactgtta tccggtaaaa
    15001 aactgacgat tcatgatgag cgagaaataa ttgatatctg ctcacgaagg agcgcaacat
    15061 atcatttaac cagaagtaat atccctaagt aatctgacgc tgccaataat ccgttaaatt
    15121 aaactttcat cgttacctgt caaaaaatgt ctatatccgc aactgtgctt atgacactga
    15181 tcagtcagtg cgcacctggt gtttctcctg aaaccatgcg tgccattatt atgactgaat
    15241 ccggtgggaa tccctacgct atagcaaatg tcaccgacgg tggcagtaaa tattttacca
    15301 ctgaagagga ggctgttaat cacgcaaaaa aactgactac agataaaaaa aatttcagtg
    15361 ctggcctggg gcagataaac agccgtaact tccagacttt aaatttaact catgaatccg
    15421 ttttttctcc ctgtgcgaat attcgtgctg ctgcggcagt actgaaaacg tgctgggata
    15481 gatacgaaac aaaaggaggg gaccaacaaa agatactacg tgacgctatg agttgttatt
    15541 actccggtaa taacaaacgg ggttatgtaa aggaaagcaa tggcaaaagt tatatcgata
    15601 taattgagga aaaagccgga atacatacac attatgatat tccggcaata aagtcatatt
    15661 cagaaacatc cagccattta atggtttcca gtgatagcca tgataaatgg gatgtttttg
    15721 gtgatttcag aacacaggat tttaaggagt gagacacagc cattaattta tttattccta
    15781 ccatctttaa cctcaactga attaatttta actgattacg gagtaaaccc gtgcataagt
    15841 ctattaattt atacagaatg ttctttttat ttgtttgtgc tttttttatt gcctcaaatc
    15901 ctgctctggc agctggtgtc ggcggcctta ataaaggcac aaacttcatc ggtgaactgc
    15961 gcctctgggc gtatggtttt gttggtggtg tggccctttt acacattctc tggaaagtat
    16021 tccaggcata cacggaaatg gactcctggg ctggcgttct caaatcactg atgtatgccg
    16081 caattgccgg tggttgtgtg gtagctgctg aagcctgcta ccagattttc aaataattac
    16141 ctaatcaccg gaatacatga tgtattccgg ttcatcatga ggatcaaaag ttggataaag
    16201 aagaaattaa agcagccgaa gagcttaagg caatctttct tacctacaat ggcctgaatc
    16261 gtcctgcaat gatcaaaggt atgcccatca ttccttttat tctgtgtctg ctggctctca
    16321 tggtttcttt ttttgttggc atactgacta ttgattttta cgggcttatt attccctcca
    16381 ttattatcgg cgtaatgatc gccatcaaac aaatgtgtgc cgatgacccg aacgccatgt
    16441 ctgccagggc actaaaattt aaaggtctgt gccttaaggg atttcgttcg aataacgttc
    16501 tgaaggttga ataatatgtc gataaagaaa ataaccaata atttcatccc tccttatcac
    16561 tcacatattc atgaaaatat catctggctt gaagataaaa aactactgct cacttttctt
    16621 attgaaggca ttccctatga aagcctgaca gatgatatga ttctgaacaa ctttaacgga
    16681 caaaaagaaa cacttcttgg actctgtaaa agtgaaagag tttttctgtg gaaacatctt
    16741 gtaaaacgcg aatcacctat ggatgccaga tataaatttc ctggcaatcc ttttcttcag
    16801 cgcttaggtg atttctactg tgatactttt aacggagaaa gatcattcag aacagattat
    16861 tttctcacaa taggcattcc ttatgatgat atcgatgctg gcgaaaaaaa agctaaagat
    16921 atcatccggc agattgaaac aggttttaaa gactacaata tttatacgct tgggatcagt
    16981 gacggcggta tttccgatat ttgttcaaac tatctgaatt atttactcaa tcatgatttt
    17041 gagaaaatac cgttatctgc aacgaagatt tcagagagta ttaagaacag caaattctat
    17101 ttcagctttg atactcttga aataaaaaat cgcacaggac acgataataa attttgttcc
    17161 aattatatcg taaaagatgt accccgaggc tcgcagattg gtcagtggaa ttttctgtta
    17221 tccctgccat ttgagtttat cctgacgcaa tcctttattc ccgattcgct atcaaaatcc
    17281 agcaagaaaa ttgagcaaca gcttaacaaa ctcacttccg taaaggacgc tggtaagacg
    17341 caacacaccg aactccatat ggcccaggaa gctatcatca accaggaaac cgtttttggc
    17401 tcctggcaca gcgtcctttc cgtcttcgga aacacacctg atgaagccag tgataacggt
    17461 tctgaagtcg cggcggaatt tgtcaccagt gggcggggat ttcgttttgt tcgtgcagcg
    17521 tctgaagcac cttatgcatg gttcagtcat ctgccagcct caaaactgcg cccccttgcc
    17581 acacaacgca cgctcactaa tgtctgctgc ctgatgccgc ttcataatca cagctatggc
    17641 aaaaaatcag gaaatccaat tggtgatggc tctgccataa tgccgctgaa aaccgtcact
    17701 gatggtcttt actggttcaa cactcactac agcgcaccag ggcgtaatgt aacaggacag
    17761 atgatcgcgg ggcacggaat gctgctgggt gcgactggtg cggggaaaac cacgtttgaa
    17821 atgatggccg ccgcgtttat ccagcgtttt aacccagata tgttcgtgat tgacttcaac
    17881 cgctctaccg aactggcaat cagaatgttc ggtggtcagt atttctctct gtctgagggt
    17941 gtgtattccg gcctgaaccc cttccagatc ggagacaatg acgatcctga actgatggca
    18001 ttcctgaaag agtgggtcaa acgttgtgct gttaataacg atggcagcga ctgtagcgac
    18061 agtgaggctg aagagattga ccgcgccgtt gaaatggtga tgaaacttga acgtcgccag
    18121 cgacgtttcg ctcgtctgca atcccgtata caaagccccg aactgaacgt ccgtctgtct
    18181 aaatggtggg ataatggcgc tcttgcctgg gctactgaca gcgaagaaaa taaattcgat
    18241 cccaatcaat acagcaaaat cggttttgac acgactgtaa tcctggaaac agttaatcaa
    18301 cgagatcatc ctgcgtgtga agtcattctt tctgttctgt ttttctacaa aaaacgaatg
    18361 cagaaaggcg gtaagctcat gctctccatc gttgaagagt tctggaaacc ctgtaactac
    18421 ccaatgacac aggctttaat caaggcttcg ctcaaggctg gtcgtatgaa gggggaaatg
    18481 atgtggctca ccagtcagtc accggaagac gcgattaact gtcaaatttt cgcggctctc
    18541 gttcagcaga caccgacaaa aatcctgctc cctaaccctg atgccaaata cagcggctat
    18601 aaagagattg ggctgacaga aaaagaattt cgccagctta aaaagctgaa actcgaaagc
    18661 cgaacaatgc tgattaagca aagtggctca agtgtctttg ccaaaatgga cctggtggga
    18721 tttgacgatt ttctgcctgt tatttcctgc tcaatgaaag ggattaaatt gtgcgaaaaa
    18781 atcagggagg aaataggctc tgacgatcct gaactctggt atcccccgtt tatcgagggg
    18841 atttatcaga tagatgacgt tatacgggaa gctaacagcg atatccctga agtctggctg
    18901 cccttcatga tgaaacggct caaagacaaa ttgccggaaa aataactgtc tctgtagcat
    18961 gccgcacaag cggcatgtaa ggcggcactc tgtacttaaa tatggctttg cgggttatcc
    19021 gccgaagccc cgcgccagcg ggtactgtct tctatattct ctggaactgc gctcgcgccc
    19081 cctgctaagc caaaaccgca cgaaaccaac agccacgcgg ctgtttctgg tcgaagactg
    19141 acaatcttaa agcgagagga aaattcaatg aaaaagactc tgacagccct gtttctgggt
    19201 gccaccctga ccggatacag ttctgtggca agttctgtag gcggtgtcat cgttaaagac
    19261 cctggcagca tagctaaaac cgtcgaagta attaagaagg ctgccgatca gattgctgaa
    19321 ctgaaaaaac aggttgatct ggcaaccagc cagcttgaag cctataagca ggaagtgctg
    19381 gataccaaaa gacgactgga aggtataacc gattattccg ccatttttgg cagtgctgaa
    19441 tcctacatga aagacttctg ggaagatatg aagaaggaac ttaccgatgc tgatattcgc
    19501 agtatggcaa ctaaatacgg ctttgataca aaagagtacg accggatcaa acgccagtac
    19561 gaatcaaagt ttgaggaaat cacgaaatac gaagcgatga gtaaggacct ggagaaaagc
    19621 gcagaaaaga taaaggcaac tcaaaaggct ttcagtaaag ccgatacgcc acaaaaacgc
    19681 gaagagcttc agaataacat tcttctcgaa actgccgcca tgcagttaaa aattgcacag
    19741 aatgaggcag aaatcaatcg tatggcccgt gaacgtaaat tacgtgagga agcggcactg
    19801 ataaaatata cagaagataa cttttccttt aactgataag aaagtcacaa attctctcct
    19861 aatacataca cttttcctgt actatttgat agcatattta attaatatat gctatcagga
    19921 ggtaacttat gttcaaaaaa ctacttggcg gttttatcct tgcctttgct tttttattat
    19981 ccggttgcga tggtgataat ggtgaattag ccgggacata tatagaaaaa aatcctgact
    20041 ctggtgatgc acctcaaaaa tggattatca aatacgacaa aaaggataat tactacactc
    20101 aaatatggat agaaaaagat aagaaaggta atgagcataa cggaacaacc aactcattcc
    20161 ttattagaga tggagactgg cttgcaggaa aagaaaaaca ttttattcga gtgattgata
    20221 aaaacacgct tcagttaaga gatgatgaga acgctttgta tatccgtaca aactgaatgc
    20281 tctctgatta acccacaacg cctgtccctg tgacgggcgt tttcttttgc aggtaataac
    20341 gatggcttca aatgactccg cttttgcaaa agcgaatttc gaaatcgtcc agccgatgtt
    20401 caatgaatta tcttcgatta ttcagaacag cgtaaccaca aattttcaga ccattatgca
    20461 acttgtgact ccattgttcg gagccgccgt cattctctac ggtgttttct ttgcgtggga
    20521 aatcatgtac acacgcaagg atgatgtgat gatggaatcc gtaaagcgta tgggcgttct
    20581 gacgcttgtt tttgccatga gtaccgccgg aggtatgtac cttagctata tcgttccttt
    20641 cgtacaggga agcggtcagg aagtggcctc caggcttatt ggccaggacg gagccagtac
    20701 cggagccgtt atcgatggcc ttcttaacag actgatagat ttaggccatg aacaatttaa
    20761 ggtttttcaa aaagctgatg gctggtttga ttcaatgggg gcatggattg tttttattct
    20821 taaagccatt cttcttgctc ttgctggcgg cggatttgtt atttactgcg cagcttattt
    20881 attaatggcc gaaatgatgg tcggcatact gctttccatt ggcggtgtgt ttatcttatt
    20941 ttctgcgtgg cctaccacgc gcaatatgtt tacagcgtgg gtgggatcat gttttaacta
    21001 tatatttctc aatattgcct atgcctgtct gttcaaggtt ctgattcagt atattaataa
    21061 ctatatcaat acaaacagcg atgatattac taaaaatatc tggagcgtcc tttgtgttgc
    21121 agttgtattc ctgattggta aattcctcct tgctcaaata tcaacgctga taagtacatt
    21181 aacaggcggt gtgggtatta acggtctgac aagcgctacc ggagatgtta taaaatctct
    21241 gcgtggggct ggcaacagca cacgaaatca gcttgggctt ggctttaata aaacagcaca
    21301 ggatgcacgg gcgcgtaaca gtgaatatgc tgccgcagca aaagattatt tatcttcccg
    21361 ctttggtgga aacatcaagg ccggataatt ccccctcttt actcaatcaa tatctcttat
    21421 ttatccggct taaaaggtat gttttatgta taaatatatc attccggcag ttgtctgctt
    21481 atttctttct gcatgtaccg caacgaatcc ccctgaagca cctgaagcta caggaaaatg
    21541 ggagccatta tataccagcg tcgataacat tatgaaggga tataaataat gggtttgaaa
    21601 gaatatttta aaagatccaa agcggaagca cgtaaaacac ccgctcaaaa agaaaccgat
    21661 aaaaaaattg aaaaaaacgt actggacgca gcaaaagaat ttgcgcgtga tcgtgataac
    21721 gaactgaagc gctcactgcg gatcacgcgt tttgcgcttt tcttttcgtc cgcttctgcg
    21781 gctgcaatgg cggttgccct cgcagtcatg gccccgctga aaacaacaga accgtttctg
    21841 ttacgggtag ataaaacaac gggctacacg gatgtgatca ccccgttcag tcaggattcc
    21901 ggtacatatg acgaggcggt aagccgctat ttcctcacgc gctatctgga gaaccgcgaa
    21961 ggttacgaat ggtacacaat tcagaacatg ctgaataccg ttgaactgat gtcttcctcc
    22021 caggtattcg ccgaatacaa caacatgatt cacggcccgt tatctccgct gtataagctg
    22081 aagaaaagct acaaaattct tgttcgcgtg cagtccatca cctttctgga tgaaaacacg
    22141 gtacagggac gttttgttaa agcaatcacc gatcccgatg gcaaacccgc acctggatac
    22201 acgccaacac aatggattgc caccatcaaa tttgtatggc taaaagacaa gatcgcgact
    22261 gaagctgagc gccagataaa cccgttcggg ctggatgtgc tctcataccg tgttgatcca
    22321 gaggtggtga aatgatgatg cgaaaactcc ttttatccct ggtcatatcc ggtatcacca
    22381 tgacctgtca tgctgcaaaa acgccagcgt ccgcaggttt tgacagacgc gtacaacaaa
    22441 tcgtctataa ccccaatgac gtaaccattg tcaaaacaaa agcgggagaa gtaaccctta
    22501 tcgagcttga agagggagaa atcatcaaga gcgaagatac tggtcttgct attggtgatc
    22561 ctgaagcctg ggatccggct gtcaggggta acactatttt tctgcgtccc aaagcagcag
    22621 aacctgatac caatatcgcc attgtgacaa acaaacggat ctactccatc cagttagtca
    22681 gtaccgacca gaacccgacc tatttattgc gatacatcta tccgaaaccg ccggaacccg
    22741 tgaaacccaa tgtcatgacg ggccggagtc gtgttgtcgc gccctgtact gaagggaata
    22801 ttgtaaacgg tcgttatgag gtgcgtggta gcaggaaaat taaacctgaa gccatctggg
    22861 ataacggtcg tctgacctgt ttccgctgga ctggcgcagc tgaccttccg gttgtgttca
    22921 ggatcttacc ggacggtaag gagcaactgg ttaattacca tatggataaa aacatcatgg
    22981 ttgtgcacga aatttcgcca gcttttgtac tgcgtctcgg aggtgaggtg atggaagtca
    23041 gaacaaaccg tcatataccg cgagcctgga acgccagcgg aacactgact ggcgaaacca
    23101 gagtggagat taataacgat gaaaaataac aacgatattg tgattgaaaa actcgataaa
    23161 attgacacgc aggaggcgga taccgacgca caaaaccgga atttcatcca ctccggtaaa
    23221 aaacagggaa acaaaaaggc aaaagcggtc cttatacttg caggttttgc ttttgttacc
    23281 gtagtcattc tgacagccgt tttatcaaag gtgttttcat cctcgggaga ggaagaaact
    23341 accgaaactg cgaccacaaa agcacaggca gaggtctatg cacaaaatac ccaggcaaaa
    23401 aatcttgatg acctgaaaga aagcgtcgcc agaagcatag aggaagtcaa aaacaacgtt
    23461 aattcagaag aggaacaagc cagggaagaa gcaccaccgg aaaacaagga aaataaaggt
    23521 gtcactctgc cagcagaaaa cgaagctgat accggatata cacaaagtaa tggcgatgcc
    23581 ccacccacac cagaacagcg tcgtctgatg ggaagcgtaa tggttgatga cagccggatc
    23641 acacagtcac agcctggaag ttccggtcaa aaaacggcag gcgctacgcc agcgtcagac
    23701 aacacaaatg tttctggtga tggtgatttt ttacagggtg ctgactttgc tgatggctct
    23761 gtatcccgca taaaaaatcg tcgctttctg ctttcggctg gaacaacttt tccgtgcgtc
    23821 ctgaaaacaa tgcttgtcac cacatatcct ggtgtaacgc tctgccagtt aaccaaagat
    23881 gtgatctcgg atgacgggaa aacggtatta atccgtgctg gcgcacaact ccagggagaa
    23941 cagacaaaag ccattacaca gggaatggca cgcgtgtttg tcaactggac aatggttaag
    24001 gaccgcaatg tcagagtgcg gattaatgct ctcggtgctg acagcctcgg cgcgtcgggg
    24061 cttccggcgt gggtggatac acatttctgg gagcgctttg gtggcgcatt aatgctttct
    24121 ctcattgaag acactctggc tgccggaacc tcacgtcttt cccgcgaaaa caacagcgac
    24181 ttcactatga acaataccac cggaacgtcc agtgaactgg cgaaaaccac gcttgataat
    24241 tcaatcaaca ttccgccaac aggttacgtg aatcagggcg aaatgctgac tgtcattgtg
    24301 ccacgcaaca ttgatttttc ttctgtatat aaggtccgct gatggaaaca atcaacatgt
    24361 ttcttgaaag tacaggcgta cgcccctttc ttcaggctga ggggcttacg gaaattatcg
    24421 taaaccgccc gtttgaaatt attaccgaag gcgttaacgg ctgggaatac catgaagcgc
    24481 caggggcttc atacagtgat ctgcttgatc tctctattgc aatcaacaaa tacaacaaca
    24541 gcgcagaacc ccttgatgcc ctgcacccta tcaagtcgct ggttatgcct ggtggtgagc
    24601 gtatgcagat tatcatgcct ccggcctgtg aagagagatg tatctcattc accatacgta
    24661 aaccttccct gaggcgcttc actctggagg attacgttag cagcaaccgg ttcagcaatg
    24721 ttcgcattgt gaatcaggca aacctgagca ctctgagtgc ctcacaacaa aaactgcttg
    24781 accttctcga taacaccagg tgtgccccca gaacctggat cagtttttta gaagctgctg
    24841 tggccgccag aatgaatatt ctggttgtgg gtggtacggg atcaggaaaa accaccattg
    24901 caaaaaccat tgcagacctg tttccccgtg atcgccgcat tatcaccatt gaagatgtgc
    24961 acgagatgac gctgccaaac catcgtaatc acgtccatct gttctataag caaggcggcg
    25021 tacagccccg atacctgatt gaagcggcaa tgcgtatgaa accggatcat atctttctgg
    25081 cagaattacg tggtgatgag gcatggagtt acctggaagc actgaataca ggacacgaag
    25141 gcagcgtgac aaccattcac gccaacagtg ccttagacgc catttcccgt cttgcgtcga
    25201 ttgtcaaaca gtccacagtg gggcttaccc tggaccacag cttcatactg aaaaccatca
    25261 aaacctccat cgatattgtg gtgttcttca gacataccca tatgacggag atctactaca
    25321 acccgatgga gaaaaatcaa ctactggcag catgatgttt tcgtctgcgc cagcagacag
    25381 aaggggcgaa cgaaaactcc agggtagcac aggagccaca tcgccccgaa gcgcggcaac
    25441 gccgctactg ttatcgaccg gaaccaggtc caccttcccg ccctgccagg cccgactgac
    25501 tcaccgacaa cgatgacgct gatttgcgcc gcagaatgcc gcagttagcg ctcgcgccag
    25561 cgagtttacg ccgtccaggg tttccctgga cggggcgctg tactgcgcgt gtgagcgcca
    25621 gcgagctacg ggtgcaactc tccggtgaaa aacctcacga caacggaaaa gctgatgaag
    25681 aaaaagaaac ttgtcctgcc tgtacttata gtctggctga tggccctgac agcctccggc
    25741 tggctgggaa gttttatact ggtgaaaatc gcctcactac cgattgacaa cctgcaatgg
    25801 agtacgtact tatccacgct ggcctattat ggctggcaac catccctcaa aaaatacctg
    25861 tttgcaggac ttgcggggac tctgcttccg ttcctgattg cactcatcct gactgtcgtg
    25921 atgtttgtcg ccgttcggga gaaaaaaacg gtgcacggta acgccaggct ggcaaatgat
    25981 cgtgacctgg cagacagcgg tttttttccc gacgaaaaag aactgaaaaa tcagaaatac
    26041 ccgtcactgc tcataggaaa aatgcctgaa aacaaagggc gttttaaatc acgctatctg
    26101 caatacagcg gacagcagtt tctgatgctg tacgccccga cacgcagcgg taaaggagtc
    26161 tcgatcgtta tgcctaacgg catctggtat acagaatcga tggtgttcct tgatatcaaa
    26221 ctggaaaact ttcttgagac ggcgggatac cggaaaaatg tgttaaagca ggaagtttat
    26281 ctcttcagcc ctgacggcta cgcgcgagaa atcggcgggg aaacagtgct gacaacacac
    26341 cgctacaatc ccctttacta tattcgccgc gatccgatat accgctttgg cgatctggaa
    26401 aaaatcgcgg tgatcttgtt tccgatgacg gggggagaaa acgacacctg ggtggagtcc
    26461 agtaaaaatg tctttatcag tctggtgctg tggttactcg ataccgaact tgagggcaaa
    26521 tttaaagtta atatgaactc ggtgatgaaa ctgagtattc ctgctgatgg ttctggtctg
    26581 gcaaaatgga tgctgaacga gatcagaagt cggggtaatg aagtactcac tgaacgctgg
    26641 aaaacgtacc atgctttacc ggaagaggaa aaacagggca aagccccctc cacctatccg
    26701 ttaagcgatg acactatccg cctgttccgc cagttcgcag ccaaagaacc caagcaacag
    26761 gaaagcatca tgctcgactt taaccggact atgtcgatgt ttgccaaccc tgtagttgct
    26821 gcggctacgg aagataacga ttttgatatg cgtgatgtgc gccgtaaaaa aatgacgatc
    26881 tactacggca tttctccagg cgcattaaag caatatcaac gtctgactaa cctgtttttt
    26941 tcacaactgc ttaatgagaa cgtgcgtact ctgccagagc atgacgcaac actgaaatat
    27001 cagtgcacac tcatgctgga cgagttcact tcaatgggcc gcctcgacgt ggttcaggtt
    27061 tccctggcat tcacggcagg ttataacctg cgttttgtgt ttattctgca aaacagggaa
    27121 cagttatttg atgaaaaaca tggttacgga aagatgggag ggaacaccat tctgaaaaac
    27181 tgtgcggttg aaattttcta tccacctaaa aaagtggatg aaagtgtaaa agatgtgtcg
    27241 gaaacaatcg ggtattatga ttttgtccgc actcaaataa gccggacaac aggcaaaaat
    27301 cccagcacta cccgcaataa agttattgaa aaacgtgctg ttatgctacc gcaagaaatt
    27361 actgatttac gggatcagaa atttaaagta aaaggaaaga aaacaagttt ttccttacca
    27421 caaatcgtaa tgagtgagtt ttgtcgccca tttattgcac ataaaattgt cagtttcgat
    27481 caaaaaaatg agtccttttt tgttaacgca agaaaatatt cccgaaaaaa tatcatagac
    27541 acacctgtac ttgatattcc acagaatgaa gtggttcgtc agttaatgct ccagcgggaa
    27601 agggaggaag catggaaggc gacgcaacct gcttttgata aagatgattt tgatgatgat
    27661 aaggaatatt ttcctggtga tgatgatggt ggtggcggtg gtgattaacc atgatgccgc
    27721 tttaagcggc atggaaggcg gcactctgta gctcaatatg gctatgcgac ttatccgccg
    27781 aagccccgcg ccagcgggta ctgtcttcta tattctttgg aactgcgctc gcgccccctg
    27841 ctaagcccaa ccgcacggaa tttacagcca cgcaattgtt tctggtcgaa gaccgacaat
    27901 gccgaagcga tattttcaat atccctgaca attcacggag ataaaaaatg aaatctcgat
    27961 tgctcatatc aatcacgcta ctttctgctc tcactttcac tctatctgcc agcgccaaaa
    28021 taaaaggact atccgcagcc ggagatccct gtgaggtact tgtctgtatg gccgaaaaag
    28081 tacagggagc accacaacca ggttgcattt cggtgaatca gagattcttt aacatccgcg
    28141 tctacacacc tttttataat cctggtgcaa cctacggcgc aagacagaat tttctttata
    28201 cctgttcagg ttcgactgtt aaccaggcaa ccttaaatat gattatgcaa cgttatggca
    28261 gacttttttc tggttaacac aggtaatgac tcattcttac ctttcaagga aaatatttaa
    28321 catcactcag tcaaaaaaac tgaaggaacc ataatgagct ttataaaaat aaacaggcaa
    28381 cgtgaattta tggagcgaag taattcacca cgtcttgtac tacgaaaaga aaagagaaga
    28441 aacggtattg taaattgtta tctttccaag acggtaagag gcgacgatac attcattacc
    28501 atagagttaa accctgaaca caataaaata agattaaaaa caaccaattc accagaaggc
    28561 cataaaatga gtcgtggttg tttttctctt actaaagttt tcgaatatat ttttcatgaa
    28621 aaccttcacg aagacagaat tattattgaa ctggagaaaa aagaaaatgg gtggtggtat
    28681 ggaaattact aaaaacagag aaacccgatt tgtaactctt gcatcagact ttaacaatat
    28741 taaagaaatt tattctgatg acgagttaaa ccgtttgctg gaagctgtta agaattgccc
    28801 gaccataatt gtgttgggtt gcgcgaacaa ggggaaagaa acgccactta tggcttcttg
    28861 tttaaggact acaccagagc gtatttcagt tgaagaattc aatgaaatta aagctatttc
    28921 ttctaaacag aaatgacaag taaacgccac agattccaga ttttaatctg gaatttgtgg
    28981 ggttgaacaa ccgaagggcg ttagtgcaca ttttgggtaa tgaattatca gctcaggaac
    29041 aaaaacgcgg tttgtgggcc gatgcaaatc cggttccacc gtgggagtgg cgatacaaac
    29101 ataactaact gatagaggtt tttatggctt tatcccccaa agaaaagaaa gagttacaac
    29161 aacgcttcga aaagcaatta ctacctgacc ttgatctgtc atttgaacta aatggcgaag
    29221 tatataaatt taattttgat aaaaataagt caggtgacta tatcgacagt tttacacaag
    29281 cagtatgggg aggttatcag gatgccgagg aatggaaaaa atatggtaaa catcaggaga
    29341 cagaattacc tgaataccct gaaccagtcg agggttcgtg cgactgtgat aaatgtaagt
    29401 cgatgatttt tcataaagag aaaaatattt atgtttgcct ctcctgccat aaacaaacag
    29461 agccatttct tccaattgac tggaactcaa acgattacga ttggctacct tatgaagatg
    29521 aatactaatg ctgaatactt cacagtcata atggatgaaa tggcggcata aaattaagga
    29581 ggcttaaatg aatatccatt tattttcaga agtattgttc tgcgtatggg ttatcgcttt
    29641 gattgtgatt ttatttatag ttgttaaata ttacaggcgt gtccattaca gactcaattc
    29701 actgtctgaa acgataaagc gcacacaggg cggtgttaat aagcgaatat ctgaaaacag
    29761 agaactgctt gaacttataa agaatcaaca tcctgaaatt ctggatgaat atccgtgggt
    29821 aagtggttgg cttgattcac aggaaaagtt tttggtcgct cttgctgata agtctggtat
    29881 cgatagaaac agttgagata gttatcattt tttattagcg atactaatga ctctgtcttt
    29941 ttatctttgt gcaatattaa agtcttcttt ttaaatcaga ggataagaac aatgatgaca
    30001 aaatatgata aggaacgatt caataaaaga atcagtgatg aagtacagat tagtgctgat
    30061 atccgtatta atgacttcat gacagaaagt gcaacttacg tcatcataac ggaatcatcc
    30121 ctgtatgaac gaatctgtca gtatgctttg caacatggtg aagatttgca ggggatgttt
    30181 aaggatgaaa gatatgaata catgtcctgc tttgttcgtg atgtagcagc attcagagct
    30241 aattttgaaa gcgaagaaat gttaaaaccc ctgtttaatc atggtaaagg tgacactgtt
    30301 gaatttgtta tcagtgttcc tgaaaaaaga gtcaaggatt acgggggtgc agaatctgta
    30361 gagactaaaa aaagatgatt acgataataa taggtgtatc tagagctatt aattataaaa
    30421 cggcggaaat tatccccccc attccctgaa tggaagagtg gtcattatat cgaaattcca
    30481 ggagagggta ttgtattcac agataaaaag aatgatgacg gtagtcgttg ttattctggt
    30541 gtcatcgaat ctcattacat ctccgatgac tgggcgttta tcctttcaga atacaaaaac
    30601 tggctgaaag acaaggaaag aaagttttct tatctgtttg tcttacgcaa tggtgaactt
    30661 gttcaccatg aggtcaataa atcagagctt ctttgagtac gtcaaagata aaggaaatta
    30721 ctatgtatat acatattgtt cctaaacttt atcatctcat ggctaataaa tgtagcctta
    30781 aatctatttc catacctgag tttgatttta taattgatag tgaatcattg tctgtaggca
    30841 gaccgtggcc taacaaatgt ttatgggttg gaatgcgaaa aggaagaaag tcagtcaatg
    30901 gcctcgtttt gcaaacagat aaaaaattaa gatggtttac tactatatac acctgggata
    30961 ttgaagatat gggattgatt tatcatcggg taaataccta tatagaagat gatgattttg
    31021 atatggtatc acaggaaatt cttttaaatg gttcttttga taagtggagt aacagggttc
    31081 attcagctta tgaaaataag cctccagcca gaatccagcc caaaatggag tccttattaa
    31141 ataaacctgg tgaaaacagt catgatgtgt gggaagagtt cgaatggggt gacttccttc
    31201 tgtccagaga agaaagcctt ttacttcaca ctattcagtc tgaacgattg agtacagatt
    31261 gctctttatt caaaagacaa ccgtctattg aaagttcatt agttatttaa aggataataa
    31321 gatgagactt tttatcgcag aaaaacccgc agtagcaaat gatattgtta aggctcttgg
    31381 gggcaatttc acccgtcatg atggctggtt tgaaagtgat aatgccattg tgactaactg
    31441 ctttggtcat attatcgaat cacagccacc ggaaaactat aatcctgatt acaaagcctg
    31501 gaaagttgaa acactccctt tacgtcttta ccccgtcaaa tatcagccag tagaaagtgc
    31561 cgcaaagcag gttaacacaa ttcttgagct tatcagacgt ggagacgtga ctgaaattgt
    31621 tcatgctggc gaccctgatg aagaaggtca gctacttgtt gatgaagttc tggaatatgc
    31681 ggggaacaca aaacccgtta agcgcgttct gattaacgac aacacgcttc cggcagtgaa
    31741 aaaggcactg gcaaatctta aagataatcg tgatttcaaa gggctttacc ttaaggcgct
    31801 ggcgcgttca gttgctgatg ccgtctatgg cttctccatg acgcgtgctt acaccattcc
    31861 tgcaaaagcc agaggatatc agggcgttct gtctgtcggg cgcgtccaga ctcccgttct
    31921 tggcctgatt gtgaatcgta cccgtgctaa ccagaaccat aaatccagtt tttactacac
    31981 catgaccgga gtctttcagc gtggtgctga tgttatcagg gcgaactgga agccagggga
    32041 atttgctccg ctgacagacc gtaaattact tgagaaagcg tgggcagacg gaacggcagc
    32101 ctcccttgca ggaaaaccgg ctacagttga agcggcagca actgatgata aaaaaacggc
    32161 tgcaccgttg ccctttaacc tggtacgact ccagcaatac atgaacaaaa agttcaaaat
    32221 gacggcacaa aaaacgctgg atattacgca acaattacgt gaaaaatata aagcgattac
    32281 ttataaccgc tctgattgct catatttatc tgatgaacaa ttcagcgaag cgccgcaggt
    32341 tatcgatgcc ctgaaatcag tctttcctca gtcgctggat attgattctt cacgtaaaag
    32401 caaagcgttt aacagtgcaa aggtgactgc gcatactgcg ataatcccga ccgccagtgt
    32461 gcctgatgtt aacgcactca gcaccgacga gcgcaatgtt tacctggcga tcgcacaaca
    32521 ctatcttgtt cagttcatgc ctgaaaaagc ataccaggaa gtatcggttg ccattcagtg
    32581 tggtgatgag tcgttctatg cccgtgccag aaaaacaact gacagcggat ttgaggcgtt
    32641 tcttggcgcg gaaaccacag acgaaggtga atcagaagat aatgatgatt ccgcttttga
    32701 acagctttgc aaaattcgca ctggtgaaac actgacaaca aaagaagttg ttgttaatga
    32761 gaagaaaaca acaccgccgc cgttattcac cgaagcctcc ttgcttgctg cgcttgttcg
    32821 tgtcgcggat tttgtcactg atccagtgat taaaaaatta ctgaaggata aggataaaga
    32881 caaaaaagat gaacatggcg gtattggtac gccagctaca cgcgctgcca ttctggaagc
    32941 gctgaagaag agaaattata tcacgctgga aaaagggaaa cttattccga cggataccgg
    33001 atgtgcgctt attgatgccc taccaggtat agcggttaat cctgatatga cagcattatg
    33061 gtctgaaaaa cagtctgcca ttgaaaatgg cgacctgacg gttgaacagt ttattaatga
    33121 tctgtacggt gaactgacag gcatgatttc tgatgttgac ctgggcgaaa tgaaaattga
    33181 acccgcagcg ccagtgggac tatctcaacg actggattcc ccctgtcctt catgcggcaa
    33241 acaaattgtt atcaggccga aaggctattt ctgtaccgga tgtgaattta aaatatggaa
    33301 gaacttctct ggcaaggctc tttctgataa gcaagtcgaa acattgctga aaaaaggtat
    33361 tacaggcgag ttaaaaggat ttatcagttc caggacgaac aaagagtttt cggctaaggt
    33421 taagctgatg gataaggcaa ccggaaagtt ggggtttgag tttattccca aaaagtagcg
    33481 atagttgata aaaattaacc atcgggtaaa ataaccttga tacatctgga tgggtagcaa
    33541 tggtaggtgt aatgtgaaaa cgggtaaacg agtgaatttc agtcttgttt aggtacacct
    33601 gaagattccg attaactagg gatggttgtc agcctttact ttcccactca atgatgccgc
    33661 gccagcggca tggaaggcgg cactctgtag ctcaatatga ctatgcggct tatccgccga
    33721 agccccgcgc cagcgggtac tgtcttctat attctctgga actgcgctcg cgccccctgc
    33781 caagccaaaa ccgcacgaaa cctacagcca cacggttgtt tctggtcgaa gaccgacaat
    33841 gttaaagcga ttttttagag cgttaaccac aattcctgtg gataactttg cacaaactcg
    33901 ggggacaaaa cacggttaac cagcttactg gcggaaatta ttggctgact attttttaat
    33961 caaagtcaca tgaatggagc cttttccgca ttaatcaaaa aaattgacaa cattagctgt
    34021 cacaacaaac catctaacct catgaaaaat aaacaatcat ctgattactg gcattgacat
    34081 cgagcagctg gccaacacgc tgattatcag cgaccgcaac aaaaaaattg aaaggaaaaa
    34141 ctgatatcat tattaaaggg cagaggtggt taacccctgc ccttagttac gtttccgctg
    34201 tcatcaatca atgaactggc gaatcagttc cagaaccgca atcagcgcgt ttgcaacggc
    34261 gacgacggct ctgagaacga tgattaacag gttaatcatc cgtctctcca tcaatggagt
    34321 agagacacca ttgcttactc atacattgcc tgtcacaacg gctctcgtta tctgttggat
    34381 attgagagtc ccactcctgc cagagcactg aatacagcac cacctgttta ttcagccagg
    34441 gctttagatt cattcatacc gcagccagcg gagcaaaagc agaaatccca aacatcaccc
    34501 aacaaacctc cgaagtataa ccagataagc aggcttttga catagcagtg tatctgactt
    34561 actatacaaa cgttggatat tgagagtcat cctccggtcg gagccaccaa ctcaaggccg
    34621 gaaaacaaaa aagcccgatt accagtcggg cttttttgtt tctgattaac gggaccgcgt
    34681 gttatccctg tttctttccc gttcacgctc atttttcaaa gcgttgtcat aactcactat
    34741 ttcacgcttc acggcctcta ccgtttcagg gtttttcatt gctgccttga gatcgtttac
    34801 atcctcaaaa cgtatgaatt tgagcgtatc tttgactgtt tgtttgtcaa tattcatttc
    34861 cttcgccagt tgcttagaaa ttcgctcaat ttctctttcc atcgcttttt cactattgac
    34921 agtccgcttt ttcttttctg tagtattctc gtttttttcc tcgactgatt tagctttttt
    34981 ctgcccttgt tgttcctcaa aggctttaat ctgttcatta atcgtctgag cacgagccga
    35041 aatactgtta acccctttcg aaagcagatc aacctcttct ttcggcgcac cattttctac
    35101 agcttcattc agtaaacgct gggcttcctg gtgctggctg gcaataactt ttgcctcttc
    35161 ctgtagctgc tgtagcgatg cgttattttg ctgatgagga ttttcagtgg ctaacgcgga
    35221 tttgctttgc tcatctccgg tctgtttatc ctgaccagta gtcacgtctt tctctgttac
    35281 cggcttctgt tgctgcgctt cttgtggctg ctgctcgcgt tcttcagaat gagccgaaac
    35341 gttctgctgc acagactcct gtttttccgc ttcctgtacc acggatgttt cagacgtgga
    35401 tttttgagaa acagtttctg acttaacagg ctgttccggc actgcatcac gcgatttatc
    35461 atcattacgt acagattccg ctacttcggg cgttctttcc ttagcctggg tgtctttctc
    35521 gactgaatta acctgctctg ctctcttttc ctgctcctgt tctggtttct gtccggcgac
    35581 ttgttgctga cgctctgcct cgacgctgtt ttcatgtcca tgttcagctt tcagttcctg
    35641 gaggcggacc tgcatcattt catgatattt ttcaggtcgc agaataacat tcatcccctt
    35701 ctcggctgcc gccattgcac aggctacctt aaattcttcc gagccattga gtttcagcat
    35761 cccgccaaat ttctcttttg cataaagaag ggctgcttca gctttatcct gggtaatttc
    35821 accatttaca cgaacatgat ttcctttatc agtgaaaaca gtctgtgttg tcttcggatc
    35881 aaggtaacgc gtttcttttg ggctgacctt atgaggaaca agatcggtta gttcacgttt
    35941 tacataatcc tgaacttgct gggattgctg tactcgctta actacgctga aaacttcctt
    36001 ttcattaccg gaagaaatgc tgtttccatc aaccggatta cggattatct tttttaaaga
    36061 atcacctaaa gccatatcat atctcctgtc tggattccaa cggtgatagt atgagtttat
    36121 ttcgcgaaaa catgcctttc gaattgagtt tatttcacgc tctctttcca gctttttaaa
    36181 tataatgtac cctctagcaa cctcccgttc ctgcttgttt cttatcgcca gaagccctct
    36241 gagttctgcc ctgtaattag ctatattatc attatgctta tatttaatct cattatttac
    36301 ccaaccttta tgttcattta aacaaataaa tctttctttt gcctgttgac gcgtaagatt
    36361 tacacgggta ataacagcat caaaaggttt gttatctttc tgatcctgcc agatatcaag
    36421 taatacctct tttgcctcat cccattcaag attcagatgt ttagttaaaa aatcactggc
    36481 attcatattt cgattgccaa ctctgaaacg cggagatcca tcctgtccat aggtgacagt
    36541 atgctttgat ggatctatgt taaagcgtgt ttgcagattg gacaaataac gttcagcatt
    36601 aatttctgtg cgaatttgtt tcaaaaaagg taaatcatca ctgcgctgaa tcgccgcttt
    36661 aatatttgat tcaagcgctg actgaactac tcttcttcct gctctatcct gcaaccgtcg
    36721 cacatatcga tctggatcga ctttttgtcg tccagccgtt gataaataac cgtattcatt
    36781 ttgctgcaat aacaactgag atttattaat gcttttccca cctgatcttc cgtgaagtcc
    36841 gtaaaccagt tcgcgtgctg gcagacgtgg caatccgatt gtcttaccgg atacaaactt
    36901 tccggtattg tatttaatac cggactggtc actattctgt cgtcggtcct gtctttgaag
    36961 gttgtgtttt tcgtcataac gattaattac ctttttcaga tacatttgct tatcttcacc
    37021 agcaagtgct gcatattctt tacgaatttt ttgccctgcc ggatgaatat gttttatctc
    37081 atgactgacc gaactcttcc actctttcag acggctctca atctgcgtct ttgtgggttt
    37141 taaaagtggg atagaacgat cagatatata ctgtcgtgaa aataaaggac ttttgagtcg
    37201 agtaaactta gagtctcctt caagtttcac accaaagtag cgattagact cccccttatt
    37261 atactcctta acctcaccaa aacgggaaag ataatcagca aattgttttt ctgttcggat
    37321 attctctgta tccagtgcat caaaaaccgt ggttttaaag tcactatgcc gctcacggta
    37381 aaaatcgcct ttagccctgg ataatacatt agcatggtta tgatcgctaa ccctgacgcc
    37441 atctttagga gaaacaagat tgtatttatt attgatatgc tcctgtattg catcaagata
    37501 ttgtatataa tgttctgtca ttcctcttgg attcagctta ctccctgtca ccagattaac
    37561 ttcaggaata acaatgtgaa tatgaggctt acgctcaacc atttctcctg ttttataatc
    37621 aggcatttgt tttatttttg gaagatgcgc ttccgcataa aaattatatt catcttcatg
    37681 ataagcattc atcatgaggg acttgtattc ctggacgaca tcatgcaact tagattcaga
    37741 aacattatct tcaaaaaagg ataacgtaat gtggatataa cgttcctgac cattatcagg
    37801 tatggtgtca ataactgatt cagtcaatgc aagatcacca tccagaacta accgatggtc
    37861 aatttcatca cgggtgtatt cccttccttg cttataacct ttttcaaggt attcgcatac
    37921 acccttattg ccaccgttga ttctgactaa catttatcta cccctgttac aaacgcatcg
    37981 cgaatattaa caaggacatt aagatagcgt aaatatgtac tttctgaaat aaaaccatta
    38041 cgatattctg tattaatctt tttagctatc tgattaatat tattgcttga cttattaaag
    38101 agtcttagca attccctgta atctgtcggg cgcttgggtt tcaaagccgg gatatcatca
    38161 agacgattca ggatgagcat acgaaaaaac tcactacgac taacatccag agatgttaaa
    38221 tattgatgaa atgactgata ttcctcatca gtcaatcgca gtgaaatcac atgttttctc
    38281 tccacatttg atgtggaagc tccagccttt ttccctttat cttgttttgt catggttcgt
    38341 agaacctcca tatagcgacc tggttgtaga gatataccgg ggtattaggg ggagatcccc
    38401 ctaagcacgt cgattatacc gaaatttatt tcgtgtatac atcgctctcg tgcctgtctt
    38461 ttaggagaag atcaactcga cgaatcacag cccacaaaac aacccacata atcacccaca
    38521 cacaaacaca atcaacccac aaaacaaccc acacttaaac ccacaaaaaa acctacaacc
    38581 atgttgacaa cccacaaaac aacccacata atcacccaca caaacaaaaa gcaaattgat
    38641 ttgaataaaa tattgtgcca atgtaggagg taataagaga ggatatataa caaatgaaaa
    38701 agatcgatag tttattatct ccaatatgcg aggcgataaa agaaacaaat tcaaaatcaa
    38761 aacaggctgt cttcaatatg tatttatcga acatacttga agcaaaaagc tattatacat
    38821 gggagcaaat aacagattac ataaataaca aaaccaagac agaactagcc caaaagactt
    38881 atataaacat gaccgagagg gccaaaaaaa ggcaaaaatg cactgggcaa aaagacctaa
    38941 aaaaaagcac caatatgatt g
//
LOCUS       DAZXUL010000030        38250 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZXUL010000030 DAZXUL010000000
VERSION     DAZXUL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38250
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.030"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..74)
                     /locus_tag="ACH3L1_001478"
     CDS             complement(<1..74)
                     /locus_tag="ACH3L1_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HFT4076274.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="ACH3L1_001479"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="ACH3L1_001479"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFT4076275.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="ACH3L1_001480"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="ACH3L1_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFT4076276.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="ACH3L1_001481"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="ACH3L1_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFT4076277.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="ACH3L1_001482"
     CDS             2704..2940
                     /locus_tag="ACH3L1_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFT4076278.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="ACH3L1_001483"
     CDS             2943..3800
                     /locus_tag="ACH3L1_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFT4076279.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="ACH3L1_001484"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="ACH3L1_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="ACH3L1_001485"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="ACH3L1_001485"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFT4076280.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="ACH3L1_001486"
     CDS             complement(6415..7311)
                     /locus_tag="ACH3L1_001486"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFT4076281.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="ACH3L1_001487"
     CDS             complement(7360..7599)
                     /locus_tag="ACH3L1_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFT4076282.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /gene="nemR"
                     /locus_tag="ACH3L1_001488"
     CDS             7702..8301
                     /gene="nemR"
                     /locus_tag="ACH3L1_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFT4076283.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="ACH3L1_001489"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="ACH3L1_001489"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFT4076284.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="ACH3L1_001490"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="ACH3L1_001490"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFT4076285.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="ACH3L1_001491"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="ACH3L1_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFT4076286.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..15224
                     /locus_tag="ACH3L1_001492"
                     /pseudo
     CDS             10766..15224
                     /locus_tag="ACH3L1_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15268..16476)
                     /locus_tag="ACH3L1_001493"
     CDS             complement(15268..16476)
                     /locus_tag="ACH3L1_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFT4076287.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16499..16909
                     /gene="tnpA"
                     /locus_tag="ACH3L1_001494"
                     /pseudo
     CDS             16499..16909
                     /gene="tnpA"
                     /locus_tag="ACH3L1_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="ACH3L1_001495"
     CDS             complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="ACH3L1_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFT4076288.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17705..18532
                     /gene="mepH"
                     /locus_tag="ACH3L1_001496"
     CDS             17705..18532
                     /gene="mepH"
                     /locus_tag="ACH3L1_001496"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFT4076289.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18660..19241
                     /gene="sodB"
                     /locus_tag="ACH3L1_001497"
     CDS             18660..19241
                     /gene="sodB"
                     /locus_tag="ACH3L1_001497"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFT4076290.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19387..20556)
                     /locus_tag="ACH3L1_001498"
     CDS             complement(19387..20556)
                     /locus_tag="ACH3L1_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4076291.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20722..20811)
                     /gene="cydH"
                     /locus_tag="ACH3L1_001499"
                     /gene_synonym="ynhF"
     CDS             complement(20722..20811)
                     /gene="cydH"
                     /locus_tag="ACH3L1_001499"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFT4076292.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            21110..22135
                     /gene="purR"
                     /locus_tag="ACH3L1_001500"
     CDS             21110..22135
                     /gene="purR"
                     /locus_tag="ACH3L1_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFT4076293.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22132..23064)
                     /gene="punR"
                     /locus_tag="ACH3L1_001501"
     CDS             complement(22132..23064)
                     /gene="punR"
                     /locus_tag="ACH3L1_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFT4076294.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            23177..24388
                     /gene="punC"
                     /locus_tag="ACH3L1_001502"
     CDS             23177..24388
                     /gene="punC"
                     /locus_tag="ACH3L1_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFT4076295.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24679..25827
                     /gene="cfa"
                     /locus_tag="ACH3L1_001503"
     CDS             24679..25827
                     /gene="cfa"
                     /locus_tag="ACH3L1_001503"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFT4076296.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="ACH3L1_001504"
     CDS             complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="ACH3L1_001504"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFT4076297.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26723..28096
                     /gene="mdtK"
                     /locus_tag="ACH3L1_001505"
     CDS             26723..28096
                     /gene="mdtK"
                     /locus_tag="ACH3L1_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFT4076298.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28137..29393)
                     /locus_tag="ACH3L1_001506"
     CDS             complement(28137..29393)
                     /locus_tag="ACH3L1_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFT4076299.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29701..29777
                     /locus_tag="ACH3L1_001507"
     tRNA            29701..29777
                     /locus_tag="ACH3L1_001507"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29735..29737,aa:Val,seq:gac)
     gene            29782..29858
                     /locus_tag="ACH3L1_001508"
     tRNA            29782..29858
                     /locus_tag="ACH3L1_001508"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29816..29818,aa:Val,seq:gac)
     gene            29966..30271
                     /locus_tag="ACH3L1_001509"
     CDS             29966..30271
                     /locus_tag="ACH3L1_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFT4076300.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30397..31819
                     /locus_tag="ACH3L1_001510"
                     /pseudo
     CDS             30397..31819
                     /locus_tag="ACH3L1_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(31831..32643)
                     /gene="ydhT"
                     /locus_tag="ACH3L1_001511"
     CDS             complement(31831..32643)
                     /gene="ydhT"
                     /locus_tag="ACH3L1_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFT4076301.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32647..33432)
                     /gene="phsC"
                     /locus_tag="ACH3L1_001512"
     CDS             complement(32647..33432)
                     /gene="phsC"
                     /locus_tag="ACH3L1_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFT4076302.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33429..34097)
                     /locus_tag="ACH3L1_001513"
     CDS             complement(33429..34097)
                     /locus_tag="ACH3L1_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFT4076303.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34161..34799)
                     /locus_tag="ACH3L1_001514"
     CDS             complement(34161..34799)
                     /locus_tag="ACH3L1_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFT4076304.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34812..36913)
                     /locus_tag="ACH3L1_001515"
                     /pseudo
     CDS             complement(34812..36913)
                     /locus_tag="ACH3L1_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(36934..37560)
                     /locus_tag="ACH3L1_001516"
     CDS             complement(36934..37560)
                     /locus_tag="ACH3L1_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFT4076305.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(38016..38225)
                     /gene="fumD"
                     /locus_tag="ACH3L1_001517"
     CDS             complement(38016..38225)
                     /gene="fumD"
                     /locus_tag="ACH3L1_001517"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFT4076306.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9208 a         9992 c         9367 g         9683 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccagct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccaac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctaggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccacaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacagcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgttttaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatgga gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctgggctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    15361 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    15421 tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg
    15481 ctggcttgtg tacgctggcg gaacagcaag cacctgaccg ccactccaga gctgcttgta
    15541 ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
    15601 ttttgcccgg acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt
    15661 gctgaccttc aaatcctcaa tgactatcat tgcgtggttt ttgctgacgg tcgttgtgac
    15721 tttgtgaagg tagtccctgc ggatatttgc gatacaggaa tgcagtcgct gtattttgcg
    15781 tttctgcttc tgccagttgt tgctgaattt gaccttccgg cttaactgtc gctgaagtct
    15841 cgccagcgtc ttctggtttt tttgaaaact gtttacaggc tcaaagactg tgccatctga
    15901 cagcgtggcg agtttagcca cgccagcatc cagcccgatc attgatgctg aagggtgaac
    15961 cggagttgat acttcacttt ctgtctgaat actgatgtac cacgtaccgc aggactggct
    16021 gacagtgaca tttttcacaa ctcccgtgac ctgacggcta ttccggtagc gcatccagcc
    16081 aagtttcggc agaaaaatac ggctgttttc ctgatcgagc ttaacgtcct gcgggtagcg
    16141 gaatgcagca ttctgtcccc gctttttgaa tcggggaaaa gcagcccgct tctggaagaa
    16201 gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc aatggctgtg agggcgaatc
    16261 tttaagccat tgcgtttcag tggcgttttt ccactcaacc agccaggaag ccattttccc
    16321 gtaagggatg tatttattac cggcctcatg attctcattc tgaagcgcca gagcacgatt
    16381 gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccaccggg
    16441 tcttaactgg aatttaaatg cctgtaatcg tttcacaggt caaatgatac tttggtctgt
    16501 gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct
    16561 ggtctttgtc accaaatacc gacgccagat ttttgaccat gacacgacag aaaaactacg
    16621 cacttacttt tcaaatgtat gtgctgattt tgcagctgaa ctggttgaaa tggatggcga
    16681 accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat ccagtctggt
    16741 aaacagcctc aaaggcatat cgaataggtt actgcgacga gatcgaccag atattgcagt
    16801 caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcgaagg
    16861 tgcgccaata tccgtcatcc gccagtacat tgaacaacag caaacacctg gtcaggtgga
    16921 aaaccgcgcc ttatatcccc gccctgaagg acgaggtttt acggcgcacc ggataaagga
    16981 ctgacggatg cccgttcgca tccgtcagta ttgcaggacg gattattccg cgtccggctc
    17041 ttcagacttg tatttagcgg cagtttcttt gatcagctgc tgcagttcgc cacgctgata
    17101 catttcgatc acgatatcac aaccgccgac cagctcgccg tcaacccaca gttgcgggaa
    17161 ggtcggccag ttagcatatt tcggcagttc cgcacgaatg tccggattct gcagaatatc
    17221 aacataggca aaacgttcgc cacatgcggc aagcgcctgg actgcctggg cagagaaacc
    17281 gcagctcggc agtttcggtg aacctttcat gtacagcagg atcgggtttt cagcaatctg
    17341 gcgttggatt ttttcgatag tggtgctcat tgtcttgctt cctcaaactt ctttacggca
    17401 gtaatctgac attgtagcgg gtcagtgcgg catcggaaaa taacattttc atcacgcttt
    17461 tgctatttta tccctttgct ctatttgttg catttcaaat attcgtttct tctctgatgc
    17521 gcggcaaact ggcgtattta taaccatttt tagctttcac tgctgcattt ttttgcgctc
    17581 gccaacgaaa cgtatttttt aacaataaaa gctattaact ttctcttctt ctatgcatta
    17641 gaatcatcaa gttttgtaaa tcagacgcag gcatgataga cctgccttaa cagagggacg
    17701 ctcagtggcg cggataaacc gtatttcgat cacgctctgt gctttacttt ttaccaccct
    17761 gcctttaacg cctatggccc atgcttcaaa gcaagccagg gagagttctg ctaccactca
    17821 tatcaccaaa aaagcagata aaaagaaaag cacggcaacc accaaaaaga cccagaaagc
    17881 cagccagaaa acagcgaaaa aagccgccag taaaagtatg accaaaagca aaaccgcttc
    17941 ttctgttaaa aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc
    18001 cgtcaataaa acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa
    18061 atcgcattgt gtgaaagtca aaaatgccgc gtcaggaacc cttgccgacg cgcacaaagc
    18121 gaaggtgcaa aaagccacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc
    18181 atatcgttgg ggcggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta
    18241 cgcttataaa gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct
    18301 gcgtgatgca ggacctatcg aacgtagtga gctgaaaaac ggcgacctgg tctttttccg
    18361 tactcaggga cgcggtacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat
    18421 tcagtcaccg cgcacaggtc aggaaattca aatcacttct ctcagtgaag actactggca
    18481 gcgccactat gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac
    18541 cctgttgtta cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct
    18601 atccatacgc acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa
    18661 tgtcattcga attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg
    18721 cggaaactat cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca
    18781 acctgattaa aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg
    18841 aaggtggcgt attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc
    18901 tggcaccgaa cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt
    18961 ttggcagctt tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt
    19021 ctggctggac ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta
    19081 acgcgggtac tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac
    19141 acgcttatta catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc
    19201 tggtgaactg ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag
    19261 cattgcctga tgcgcttcgc ttaccatgcc aacgcggatg aggcgttaac agcgcattcg
    19321 gcaagataaa tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt
    19381 gtttaattag ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac
    19441 cagcaataat gccagtcccg cgacaatcgc ccccgtcacc ggcacaaagc tgtatcccag
    19501 cccagcggaa attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc
    19561 accaatattg actgatgaag acagacctgg cgcttcactg gcgacatgca tcacgcgcat
    19621 ctgtaacgga ggtacaaccg caaaggttgc tgcgccccac accaccatgc taatagctgc
    19681 gccgaactca ttgcgggcca ggaacgggat tgccagcata atcaccatca gcagcaacaa
    19741 aaagcctttc aacgtgccgt taactgaacg atctgccagt ttgccgccga gatagttacc
    19801 gatagagaat ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg
    19861 ggtaatactt tgcagtaccg gagagatata ggtgtagagg gtaaacattg caccagctcc
    19921 cagtaccgtc gtcagcaatg cagacagcac ctgcggacgc attaataccg ccagctcttt
    19981 tttcacttca ggtcgtgccc ctgtaccacc tttaggtaat gagaagaaca gacttaccat
    20041 tgaaatcact cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc
    20101 caaccaggtc gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt
    20161 ggcaactgcg ctggcctgtt tatgttttgg caccacgctt gcggccacga ctgaacccaa
    20221 accaaaaaat gctccgtgat tcaggctggt caaaatacgt gaaagcatca gggtcatata
    20281 atccggcgcg atggcggaaa gaacgttgcc tagcgtgaaa attgccatca ggaaaatcaa
    20341 cgcactgcgg cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc ccaccattac
    20401 gccaactgca taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg
    20461 cgcaatgacg ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc
    20521 aatcgccagc gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc
    20581 attacgcgat attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata
    20641 acaaaaagag aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga
    20701 acgcgctccc tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ctacgatcaa
    20761 accgaggacg ataaccgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc
    20821 tcctttttta ttaccacaca aaaagtgata ttacgcattt ttacacactg tgatgaaaaa
    20881 atctcccgtc atttataatg ataagtgttt ttaccacttc cccttttcgt caagatcggc
    20941 caaaattcca cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg
    21001 tcgtttttcc ggcgtaccgc aacacttttg ttgtgcgtaa ggtgtgtaaa ggcaaacgtt
    21061 taccttgcga ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat
    21121 aaaagatgta gcgaaacgag caaacgtttc cactacaact gtgtcacacg tgatcaacaa
    21181 aacacgtttc gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca
    21241 ctactcccct agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt
    21301 gctggcgacc agcagcgaag cggcctattt tgccgagatc attgaagcag ttgaaaaaaa
    21361 ttgcttccag aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca
    21421 gcgggcttat ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc
    21481 tgagtaccca gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt
    21541 gatggactgg ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga
    21601 aggcggctac atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat
    21661 ccccggcccg ctggaacgta acaccggcgc aggccgcctt gccggtttta tgaaggcgat
    21721 ggaagaagcg atgatcaagg tgccggaaag ctggattgtg cagggtgact ttgaacctga
    21781 atccggttat cgcgccatgc agcaaatcct gtcgcagccg catcgcccta ctgccgtctt
    21841 ctgtggtggc gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggcctgcg
    21901 cgtcccgcag gatgtttcgc tgatcggtta tgataacgtg cgcaacgcgc gctattttac
    21961 gccggcgctg accacgatcc atcagccaaa agattcgctg ggtgaaacag cgttcaacat
    22021 gctgttggat cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg
    22081 tttgattgaa cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc
    22141 cgttgcggga gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgct
    22201 gagatactca agcagccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca
    22261 acatgccgaa tccgggaagg ggttttccag ttcaagtgct acccacttcc cctcattaag
    22321 ccacggtttg gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt
    22381 tgccgatgat tcccagtcag gtacgacaag tcttttttgg ttatccagca accaggtaat
    22441 acgtttaggt agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt
    22501 atcatcgctg aacgggccat ccatcaacgc cagcgggtgg tgactggcaa caacgcaact
    22561 ccagcttagc atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt
    22621 tgcgccaatt gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac
    22681 ttcctgaaag acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg
    22741 ccgtgtacgt tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc
    22801 gttcgctatc tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc
    22861 ttctttgaga aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa
    22921 gagcggtacc gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac
    22981 acgatgcagc tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac
    23041 ttcgagtgaa tattctgacc acatagtctg cctgcaaaat ttttgaaacc agtcatcaaa
    23101 tattaccgtt tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga
    23161 gatttagaga atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag
    23221 cgtactcggt tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga
    23281 cctgcaaacg cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc
    23341 cgcagcccag cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt
    23401 aatcggcctg acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc
    23461 cacgctgctg gtattgcgtt ttgtacaggc tgtgggggtc tgcgccgcgg cggttatctg
    23521 gcaagcatta gtgacagatt attatccttc acagaaagtc aaccgtattt ttgcgaccat
    23581 catgccgctg gtgggtctgt ctccggcact ggctcccctg ttaggaagct ggctgctggt
    23641 ccatttttcc tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct
    23701 gcctattttc tggctcaaac ccacgacgaa ggcccgtaac aatagtcagg atggtctgac
    23761 ctttaccgac ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat acgcagcctg
    23821 ttcagccagt ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta
    23881 cagcccggca gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg
    23941 ttatggctgt cgcgccgcac tgcagaaatg gcaaggcaag cagttattac cgtggttgct
    24001 ggtgctgttt gctgtcagcg tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc
    24061 gctggtcgaa atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc
    24121 tattgttgtc gcccaggcgc tgcgtccctt cccacatgca accggtcgcg ccgcagcgtt
    24181 gcagaacact ctgcaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggcttat
    24241 tagtatcagc acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt
    24301 cgcgctgggt tacatgatgc aacgttgtga agaagttgac tgcccgaatc atggcaatgc
    24361 cgaagtcgct catagcgaat cacactgatc tatatcgata tacttatact taggctgcta
    24421 acaaaatttt gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct
    24481 acgataaata ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat
    24541 ttcggcaagg gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt
    24601 ctgcgagatt tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg
    24661 accagtgatg gagaaactat gagttcatcg tgtatagaag aagtcagtgt accggatgac
    24721 aactggtacc gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct
    24781 gccccggcgg atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaggaaggc
    24841 tctttggggt taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg
    24901 ttttttagca aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac
    24961 acgctgcgta ttgccggcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata
    25021 gtcggcaagg agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc
    25081 atgcaatatt cctgcgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg
    25141 aagctcaaaa tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt
    25201 ggctgcggct ggggcggact agcgcactac atggcatcta actatgacgt aagcgtggtc
    25261 ggcgtcacca tttctgccga acagcaaaaa atggctcagg aacgctgtaa aggcctggat
    25321 gtcaccattt tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct
    25381 gtggggatgt tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat
    25441 cgtaatttga aaccggaagg tatattcctg ctccatacta tcggttcgaa aaaaaccgat
    25501 ctgaatgttg atccctggat taataaatat atttttccga acggttgcct gccctctgta
    25561 cgccagattg ctcaatccag cgaaccccac tttgtgatgg aagactggca taacttcggt
    25621 gctgattacg atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa
    25681 attgcggata actacagtga acgctttaaa cggatgttta cctattatct gaatgcctgc
    25741 gcaggtgctt tccgcgcccg tgatattcag ctctggcagg tggtgttctc acgcggtgtt
    25801 gaaaacggcc ttcgagtcgc tcgctaaagg ctattctatc gccccctctt cgggggcgag
    25861 ttcagatcag gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg
    25921 ttctaccgta tctaccactg cctgagtttg tggatcgatt tcaatgttga cgcgtgcgcc
    25981 aagttttttc ttcccaagag tcgtgcgttc cagtgtttcc ggaattaaat ggacgcaaaa
    26041 acgcgttggc gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt
    26101 gtacaggata tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt
    26161 ttccgaggtt aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg
    26221 tccgccaatt tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt
    26281 taaatcgcca agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt
    26341 gccgttaatt tccgtcacgg tcaggcagca accgttatgc gccacggaag caccggtttc
    26401 caggccgtcc agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc
    26461 gtcaatcgac accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc
    26521 tgaaatcagt taagacattc tgttcagcac aatagcaggt ggaaaacgcc cttaccagtg
    26581 aaggggtaag aatggctatt ttttcactgg agaattaata aatcctcgct acaatagact
    26641 gaatttcccc tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata
    26701 actacaaata aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc
    26761 attagcaatc ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac
    26821 cgtgatggcg ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat
    26881 ctggcttccg gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc
    26941 gcaattaaat ggttccggtc gacgtgagcg cattgcgcat caggtgcgac aaggtttctg
    27001 gctggcaggt tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat
    27061 ccgctacatg gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc
    27121 gttgttgtgg ggcgcgccgg gatatctgtt cttccaggtt gcccgtaacc aatgtgaagg
    27181 tctggcaaaa accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacatccc
    27241 ggtgaactat atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg
    27301 tggcgtggct actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa
    27361 acgcgcccgc tccatgcgcg atattcgtaa cgaaaaaggc accgctaagc ccgagcctgc
    27421 ggttatgaaa cgactgattc aactcggttt gccgattgcg ctggcactgt tctttgaagt
    27481 gacactgttt gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg
    27541 acaccagatt gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc
    27601 agcggtaact atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac
    27661 cgctgcgcgg accgggctta tggtgggtgt ctgtatggca accctgacgg ccattttcac
    27721 ggtttcactg cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct
    27781 ggctgcgcat ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat
    27841 tggcagtggg attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac
    27901 agcttactgg gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt
    27961 tgaacctatg gggccagcag gcttctggat aggctttatt atcggcctga catcggcagc
    28021 cattatgatg atgctgcgta tgcggttcct gcaacgtatg ccgtcagcca tcattctgca
    28081 acgagcgtcc cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac
    28141 gcaggctgga gcgttgcgcc aatcccgtct tcgtctggct gtaatttcag agcgttacac
    28201 agagtttgcc gactggcatc tgttatctca acgcgtatat ccccgttaac ttcccctttc
    28261 acattgatga agtcaggtga aatttctttc gcactggttt gcaggcaaat cgctccctct
    28321 ccgcgcaggc gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt
    28381 tttccgccat tcagattgat gttaccaaca atgaaagtga cgctgtcacc ttttaccgta
    28441 ctgccgtcga gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagctct
    28501 gcgccataac caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca
    28561 accggtgcgc gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg
    28621 atcactgttc caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccgc
    28681 ccccccacat tcaccgacag gttttgcgtc ttaccggaac tgtagagctg aaggccatcc
    28741 gtccccagac gataaatgga tccattctca acaatagtgt cagagtcttc accaccgaga
    28801 ttattcatgg tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg
    28861 ccgccctgat taatctcaca attgctggct tctccacttg gatacaccaa caattcacca
    28921 ccttcatcta ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg
    28981 agaatgattt tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc
    29041 aacaaaccgc aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta
    29101 accgtagcga gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca
    29161 atggcaccgt cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc
    29221 ccaccggttg ccactttcaa ccagccagcc cccgtgacca cggtattttc ggctcttccg
    29281 ccagcttcaa caatcaactc gccttcagta agttgcgtcg cgccaaggac ctcgccggtc
    29341 ttaacaattt gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat
    29401 tcctttgctg catctgtgtg cctttattgc tacctaagtg taaaggctac ggaggattta
    29461 tccacgacag atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat
    29521 tgggttgtat cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa
    29581 tatagccaat ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt
    29641 gcctcttgcc acctttccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt
    29701 gcgttcatag ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag
    29761 tccaattgaa cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat
    29821 ggtgggggtc ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtctttt
    29881 cctttttccc tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt
    29941 atgcgttatc agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa
    30001 tggcccattt ggcgacgcaa tggctgagca gcttaaacca cttgctgagt cgattaatca
    30061 ggaacctggt tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg
    30121 gatctacctt ttcactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg
    30181 actgaaaaat ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact
    30241 tagtcaaatc aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc
    30301 ttttcatccc tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa
    30361 cactaaattc gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt
    30421 gccgggccta cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct
    30481 atttctatct tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa
    30541 aactcaaaaa tgatgctggc aaatattgcc agtattgaaa taccgccgat taattgtacg
    30601 tatctcgaat ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa
    30661 gaaaccttgc acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat
    30721 caatttttac gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa
    30781 tcatgccagg ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag
    30841 gatttaccca gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat
    30901 gaagaagaag caaccagaac gtattttccc agcccgtggt caggcctgat ggaagcaaag
    30961 gtcgatgcgt gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg
    31021 gcagtggcta ttcagcatgg ttcgttcatt gaagatgata aacaacacgt cgtttttcac
    31081 cgcgataacg caagtgaaaa gctaaatatt acgttgatgt cgcgcacggg tattttaccc
    31141 gaagccgatt tctattgccc tattccctac gagcccttac acatcgtcac cgatcaggca
    31201 ttaaatgctg agattcaaaa aggtgaagag ggccttttgg atcgggtatt tagattgata
    31261 gtagaggaaa tcaagtttgc tgatccagac tggagccaac gcatagcctt agagagcctg
    31321 aatgtcgatt cctttgctca agcctggttt gccgagcgca aacaacgcga cccatttgac
    31381 tgggcagaaa aaaatctcca ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc
    31441 tggcgttatg tcattctgcg cctgcatgaa gccgtacagg aaattgttcc tcatctgaat
    31501 gaacacgacc ataaacggtt cagtaaaggc cttgccaggg ttttcatcga taattatgcg
    31561 gcaatccctt cagagtctat tcgtcgacta ctggccttac gtgaagcggg gatctgaaat
    31621 ccctgatgtt ggcgaagatt atacgttaca gcaacccgaa gatattcgtg gacgcgtagc
    31681 gttcggcgcg ttgccctggt tgatgcacga ccagcctttc gttcagggac ttacggcatg
    31741 tgcagaaatt ggtgaggcga tggctcgggc ggtcgtaaag cctgcatccc gtgctcgtcg
    31801 gcgtctttcg tttgattaaa ggtaaagcta tcagcactca acaaactgat gctgttcatt
    31861 aagacgatag cggactccgg ttcgaatatt gttttcacct tcaatggcga cggcgaagcg
    31921 aactctttcg ccgtcatgat atgccagtgc ggcgcttccc cctagcccga gaataattga
    31981 gtcgaccacg cccgtgctca ctaccgttga attttcaccg ctggcaatga tgtgtacatt
    32041 gtcgccgctg ttggcaattc tggcgtttgc gccaaagctg gcgatttgca ccaggtcacc
    32101 attactggcg acatgacacc gttcacccaa tgtacaaacg cgtactcgca ttccggtatt
    32161 cgcaatccga ctactatctc cagcgctgct tatccgcgaa gaattaccgg ttacagcaac
    32221 ccgtgcgcgt tcaccggagc tgccaatgtg actgttaaag ccgacacttc caatccgcac
    32281 gctataaccc atactggcaa tttgcgcggc atacccagcg gttgcgattc gggcgttatc
    32341 ctcacaacag ccgatgcgag catgatcgtc gctgcgggca aactgactgc aaaatggtat
    32401 ctctccgggg tctgtggcca gtttttgcat agaggaaact tcctgatgcg cgaagttttc
    32461 atccgccagc cattttttcc acgcatactc aaccagactc tccgcccagt ccgtatatcc
    32521 ttcctcaatc agtgcatgac gaatatcggc ataactaccg ccgcgtggga aatggcgcag
    32581 gaaccagcga tacatcaccg ctccgatacg ccattctcgc aggtctgctc gtgtaatgat
    32641 cattcgttag tgccggtgat agccatcgac catgctttta aaggtttcgt gtggcgtacg
    32701 ccccgtggtg caaagataaa gatgaccgaa gataaaaaag aggcttataa atgccagagc
    32761 aaaatgcgcc tgcaataacc agtatcttac gccaggaaac acatctccca cggcttgcgg
    32821 atagagacac aacagccccg tcaatagtaa caacggcagc aatccataca tgacaccaac
    32881 ataggcgacc tgctgtaagg gattaaattt agactgtgtt gttgccggga aaggatgttc
    32941 ttccccctgc ataatgccaa acaaataaaa tcgcgtttgt tttgccgctc gttccagcca
    33001 cccctgacga cgaatgcgat agtggtgacc attatcccca acggcattga tcagcacaaa
    33061 gccgagccag catgccagta acaaaaatcc gcaaacttca tgcaccgcaa ccagactttt
    33121 aaccgcagtt gcgcccacca tcgcaaagtg atttatcagc ccacttgcca gcaacaatac
    33181 aaagagtaac gcattcgacc agtgccataa tctgaccgct ttgctgtaaa gataaacctt
    33241 ctcaccatga tcggtggctg atttcttcac gccacgagca cgaagcaagg cgtgcagccc
    33301 cagcaccaac cacatcccaa ccagcaatac tccggcaatg atcaaccaca ccggccaaaa
    33361 ttcgggagtg aactgtggca catagttcgc taactggctc tgaaactgtt cagcatgttg
    33421 cgacgggttc atacattttc ctttttaatc aaatgttgac cgaaccggcg atacagatgc
    33481 ggttttccgg caccaggtag ctgatattga tagtatttat tatcctgcaa ccacgcctga
    33541 atttctgggc tatcttcgcg accaaagatc aatgcgtgtt ccggacaggc gctgacgcaa
    33601 atgggcggaa agccttttgc cagtcgggac tcagcgcaga aatcacattt atctgccact
    33661 ttagtgacag ggttaaggta gcgaaccagg tacggacaag cgccaatgca ataactgcag
    33721 ccgatgcact gcgatttttc tacccgcacg atcccctgct catcacgcca cgatgccccc
    33781 gtcgggcata cgtcgatgca cggagcatct tcgcagtgct gacatgactg gcgaaaaaag
    33841 tgatactgcg tttcgttgtc gttatcggtg actggaatgt gtgctatcga taaacggctt
    33901 ccctgagcgg ggacgtggtt agttttacgg catgcgcggg cgcaaatatt acagccattg
    33961 caccgtgact cgtcatgaat catggcatac cgaacttcct tttcttgcct cgtgttcgct
    34021 aacagagaag acgcagaacc agtaaaaaag attactgttc ccatccccag aacaaatttg
    34081 cgtcgagtga aggacatctg attatcccct aatgcagtgg ctgcaactgt ccaccctatc
    34141 catgacatct ccctgaaatt tcaggggttc tgaaactgct ctacctcgaa ccattcgcat
    34201 aagccataag ccttgccata ggtcataaat cgctcgctaa acaagtacaa caccgccgtc
    34261 gatgactcca cctgccgtat atcggcatat tcaggtgcaa catccatcgc ggcgatcgcg
    34321 gcttcaatct gcgcttcctg gaagtaatat ggcgcttgca tcagcatggc gactttgtaa
    34381 ggacgaggat acgtctggca ctcaaaacga acggcgtgtg caatggcgcg acaaatgtcc
    34441 tgctccacaa cttgcaaaga catcgccgcg tagttttcac tcatggcctg cgttgagtaa
    34501 tagtaatcat cctgacttcc cgtcatacaa gcgatatccg cgtagtctcc gttctgctta
    34561 atgctttcca gcaaaactga cagttcctct tctgcaaccg aatagggtgg cgagagaaac
    34621 acggtgcgcg ccaccagctg cccagccgca gaatgctggc gaatgaactc agccaggatt
    34681 tcagcaggtt ctggttcggt taccgtatca acggggtgac gcatcccctg caaccattgc
    34741 cgcttcgcct cgtcaacttc gctgactttc gctaacggca attcatcctg atggttcatc
    34801 ttacccattc cttacgcagg cagtagattg tgtgctgcca gatcggcggc aatatcttcc
    34861 agccccagac gctgcaatgt ttcgcgggtt ggacaaccaa gctgtgggtc ccagcccatt
    34921 tctttgtaga acatcgtgag cgaagcatgc atatcgtcac gatccatttt gtcagtacct
    34981 tcagtaaaga ccgggatctg cggatccttg tcgaaaaccc aggaacagat aagatcgtgt
    35041 tcattacgca tatctttggt ttgcatcggt tttaccgtgt aggcacgatg caacgtaaag
    35101 atacgctctg cagctaaatc taatttttcc tgagtcatct cttcgccggt gatcgctttg
    35161 aagaatttgg cttcaagcgc cagatcgccc cggtaattac ggcttttcag cggcgaaacg
    35221 gtcattggcc agacccagtt gcacagagtg acggcgttat gcaaacagac ccgcaacagc
    35281 gaccactttg catatttaat ttttgcgtca ttgattggcg tgtagttttt ggtttcatcg
    35341 taagcatctt cagaaccaaa aagttctttc gccacttcac gttgcagttt caatggcaag
    35401 ccggaaccaa taaagttgat atgggtatgc gtcatgcagt cacggttgaa catacactta
    35461 acaatggaac cgacctgcgc tgacgcttca ttggcatggt gaaccggata gccaaacggc
    35521 gaccagagtt tatttttcgc gtagccccag tactctttac ccaaattcca gcgttctgcg
    35581 atggcatatg aaccatcagc caggtgactc agctcaccca cacgatgcgc cagacggtag
    35641 taaaaatctt taatgaagtt aacgtcaccc gcttccagtt gatcccagcg aatttctgca
    35701 tactcttcag ctggcagaac acgcttgaac acacctttgc tgtaacaata agtaaagtcg
    35761 cgatgcaact gcccgtagtt acaccatagg ccgtagtcat cgaacaaatt cagaccaacc
    35821 aggttaccaa tcacacgacc atcgtcttta tcttcaaaat ctttcggccc gttcgggaag
    35881 atggtggtat ggacaaagtt tgctacacaa gtgttaccac ctgtgctggg cacgccaaac
    35941 tctttcaccc gaggaatatt catttgggtc atacaacgga tcgggcaaga gtggcagccg
    36001 ctcattttta ctgtgtattt ctctgccgcc ggtcctaagt caaaaacgga tttataggta
    36061 cgaaagccga ccgtattctg attgcctggc ggaatttcac ccgtttcaat cggaccacct
    36121 tcagccgcgc cccaaaacag ccctttacgt gctgtccagc gtgacttggg atctgaatac
    36181 tctgcccacg attgtggcgt acttggcacg acatggttgt tattcgcacc aataagttca
    36241 gtcatcatgt aatcattgag acgcttcatc tcctgacgat cggcaatgtt gacccctttc
    36301 gtcccttcaa ccgcaatcgc tttcaggttt ttcgaaccca ttattgcgcc agttcccgca
    36361 ccgccgctgt ggttacggct attcaacatg ccagagagag gaataaggtt ttccccagcc
    36421 tgaccaatag ccgccacaca ggtttccggg ctggtcaatc gacaaatttc ttccgtcgtt
    36481 gcgcgcgtcc cttttcccca taagaaatcg gctttttcca ggctaacttt gtcatcttta
    36541 atcttcagcc ataccggtga tttcgccttc ccttcgataa taatgacgtc gtatccagcg
    36601 aatttcattt gcgctgcaaa aaagccaccc atatgggcat cgcgactaaa tttcctttgg
    36661 taaaagtaga aagtgaggtg atatttacgc gagaactaca gggggcacca gatccaggta
    36721 atgggccggt agcaaagact aatttattcg cttcatcgaa aggtttcgtg cctagcggta
    36781 cttcgtcata cataattttg tagccgaagc ccatgccacc gacaaaactt ttaaacttac
    36841 tggaatcttc gagggtaata tttcctgtcg tgagattgac tcttaatata ttacctgtcc
    36901 aaccgttagc catgattttt tcctttgcaa gattcacaca gtaatatctt tccactcgat
    36961 aatttttaac gcccctgttg ggcaggcgtt tgcgcattca ccgcataaca cacatttcga
    37021 ggatttttta ctttcggtat ttacggtggc catcatccac gggcatgccg tagtacaggc
    37081 gctacagcca atacaacgtt tatgatcgac ggtaatacag ccttctttct gctgccaggt
    37141 aatcgcacca atcgggcaga cgttcatgca ttgcggctct ttgcattgac ggcaggtgtc
    37201 cgcggtatag ttgagatcgc catacaggcc gccgccagag ccaaccccgt tgtcgccaaa
    37261 gaaataattg cgatggattt tgatacggga gaagaatgtc cctactgagc catcgttgaa
    37321 gttggtacaa gagatttcac agcggtggca acccgtgcat cgcgctcgct gggtcaccag
    37381 cacccctttg ggtgtattta tcaaccctac tgtgccgcta tcaatatctt cctgtttgca
    37441 acccaaaagt gacagtaacg caggggcaat tgttagacca gcaaggcctt ttcctgatat
    37501 acgcaagaat tctaatcgcg ttaagccaat atctaatagt ggacgatcaa ccgggttcat
    37561 ttatttcgca tcctcttcgc aaacacagtg ctgacaattc agcagcctga tattaagcat
    37621 tcaactaata cgctgctgac tctgtcccca acaccagata ttttccctaa cccgatagtg
    37681 tatgagaaaa gatttaacgc cgataaacta atcctttatg gttaggagaa aaaataatgt
    37741 gattattctg agcccttaac attgatcgtt atcaattaaa attacaaagc aagaaattat
    37801 aagcgaactg atatttatta tcatttgaaa taaatttaac ttaagcgaca atcatttgtg
    37861 acgttcattg cagaaaataa aaataacatt atcgctataa attaaaatac ataccgaaat
    37921 tcgtataatt tcagcagttg ttatttagaa tgattaactg gaccgcaact gaagcaagaa
    37981 tatcaggatg aagcttaccg cctcatcctg aatggtcatt ttaccagtgc gttaatgacg
    38041 gtttccatcg cctgcttttc cagctcactg cgctgaatgc gtttattggc taatgctgta
    38101 cgtaatacgc ctgcgataac aatatgcttt ggttcctgcc ccagatcgcg catttccagc
    38161 acaactttac cgacaactct gcacatttct cggtacagtt catcctcttt tgtccgattt
    38221 cccattcttt atacctattt atcatgccaa
//
LOCUS       DAZXUL010000031        37628 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZXUL010000031 DAZXUL010000000
VERSION     DAZXUL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37628
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.031"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..176)
                     /locus_tag="ACH3L1_001518"
     CDS             complement(<1..176)
                     /locus_tag="ACH3L1_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFT4076307.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="ACH3L1_001519"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="ACH3L1_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFT4076308.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="ACH3L1_001520"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="ACH3L1_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFT4076309.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="ACH3L1_001521"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="ACH3L1_001521"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFT4076310.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="ACH3L1_001522"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="ACH3L1_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFT4076311.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="ACH3L1_001523"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="ACH3L1_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFT4076312.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="ACH3L1_001524"
     CDS             complement(5180..5422)
                     /locus_tag="ACH3L1_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFT4076313.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="ACH3L1_001525"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="ACH3L1_001525"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFT4076314.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="ACH3L1_001526"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="ACH3L1_001526"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFT4076315.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="ACH3L1_001527"
     CDS             complement(8339..9313)
                     /locus_tag="ACH3L1_001527"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFT4076316.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="ACH3L1_001528"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="ACH3L1_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFT4076317.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="ACH3L1_001529"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="ACH3L1_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFT4076318.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="ACH3L1_001530"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="ACH3L1_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFT4076319.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="ACH3L1_001531"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="ACH3L1_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFT4076320.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="ACH3L1_001532"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="ACH3L1_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFT4076321.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="ACH3L1_001533"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="ACH3L1_001533"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFT4076322.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="ACH3L1_001534"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="ACH3L1_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFT4076323.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="ACH3L1_001535"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="ACH3L1_001535"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFT4076324.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="ACH3L1_001536"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="ACH3L1_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFT4076325.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /gene="aaeX"
                     /locus_tag="ACH3L1_001537"
     CDS             23180..23383
                     /gene="aaeX"
                     /locus_tag="ACH3L1_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFT4076326.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="ACH3L1_001538"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="ACH3L1_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFT4076327.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIYSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="ACH3L1_001539"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="ACH3L1_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFT4076328.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="ACH3L1_001540"
     CDS             26388..26660
                     /locus_tag="ACH3L1_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFT4076329.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="ACH3L1_001541"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="ACH3L1_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFT4076330.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="ACH3L1_001542"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="ACH3L1_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFT4076331.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="ACH3L1_001543"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="ACH3L1_001543"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFT4076332.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="ACH3L1_001544"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="ACH3L1_001544"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFT4076333.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="ACH3L1_001545"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="ACH3L1_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /gene="zapG"
                     /locus_tag="ACH3L1_001546"
     CDS             complement(31907..32305)
                     /gene="zapG"
                     /locus_tag="ACH3L1_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFT4076334.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="ACH3L1_001547"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="ACH3L1_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFT4076335.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33845..34273
                     /gene="rplM"
                     /locus_tag="ACH3L1_001548"
     CDS             33845..34273
                     /gene="rplM"
                     /locus_tag="ACH3L1_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFT4076336.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34289..34681
                     /gene="rpsI"
                     /locus_tag="ACH3L1_001549"
     CDS             34289..34681
                     /gene="rpsI"
                     /locus_tag="ACH3L1_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFT4076337.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35076..35714
                     /gene="sspA"
                     /locus_tag="ACH3L1_001550"
     CDS             35076..35714
                     /gene="sspA"
                     /locus_tag="ACH3L1_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFT4076338.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35720..36217
                     /gene="sspB"
                     /locus_tag="ACH3L1_001551"
     CDS             35720..36217
                     /gene="sspB"
                     /locus_tag="ACH3L1_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFT4076339.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="ACH3L1_001552"
     CDS             complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="ACH3L1_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFT4076340.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIVTYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9117 a         9414 c         9898 g         9199 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaactggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatctg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagagt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgggcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctca ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catctactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ctggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag gacaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctgggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacggcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttcccaaaa
    33721 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    33781 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    33841 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    33901 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    33961 gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    34021 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    34081 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    34141 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    34201 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    34261 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    34321 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    34381 ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc
    34441 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    34501 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    34561 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    34621 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    34681 attggcttct gctacggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    34741 atggctaaat tctgaatcag cgtaaaaaat gggaagttgc tttttgctgc cgtctgaaag
    34801 acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag
    34861 cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa
    34921 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    34981 acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct ggtggctggt
    35041 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35101 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35161 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35221 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35281 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35341 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35401 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35461 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35521 tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35581 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35641 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35701 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35761 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35821 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35881 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    35941 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36001 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36061 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36121 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36181 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36241 gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata ttcaccacga
    36301 aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa
    36361 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    36421 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    36481 gaataagact ggcaaatgac ataaacggcg cattgccaga gcccatcaca atggcacaaa
    36541 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    36601 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    36661 ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca aaaaacgcct
    36721 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    36781 ccgtcaaact tagtaacatc accaccacca gatgcagttc tgactgcatc agcccgacgt
    36841 gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa atggcgtaat
    36901 agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg atatttttat
    36961 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    37021 aagaggcgac cggaagctgg tagtggaaga agtatgtgac aattttcatt cccgcgacct
    37081 gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc gtggttgcga
    37141 taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga aataaggtga
    37201 ccatcagcaa catacccaga ccggaggcgc tggtaataaa ctgcgccatg atttggccga
    37261 tgacgtaagt tgccgacaga ataatatacg gcgagcgaat gagttttaac gggcggctta
    37321 gcaagctcac catcgcgcaa ctggctccta tgcgctccat gtagcgggca taaccgccca
    37381 ccgccataat cgacagcccc aatccggcaa tgcggttgga cagcatgcgc aggatttcat
    37441 tataaatgtc gacgatcaag tagccgctgc ttttggcggg atcgagtaca ccaccgaacc
    37501 ctaaccagac tccgcacatc atcaggaaga cacctgcggc agccagcacc acctgaggtt
    37561 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    37621 cgaacatt
//
LOCUS       DAZXUL010000032        36796 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZXUL010000032 DAZXUL010000000
VERSION     DAZXUL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36796
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.032"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            114..470
                     /locus_tag="ACH3L1_001553"
     CDS             114..470
                     /locus_tag="ACH3L1_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFT4076341.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            474..1601
                     /gene="dapE"
                     /locus_tag="ACH3L1_001554"
     CDS             474..1601
                     /gene="dapE"
                     /locus_tag="ACH3L1_001554"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFT4076342.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1629..1829
                     /locus_tag="ACH3L1_001555"
     CDS             1629..1829
                     /locus_tag="ACH3L1_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFT4076343.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1939..2637)
                     /gene="ypfH"
                     /locus_tag="ACH3L1_001556"
     CDS             complement(1939..2637)
                     /gene="ypfH"
                     /locus_tag="ACH3L1_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFT4076344.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(2711..4726)
                     /gene="tmcA"
                     /locus_tag="ACH3L1_001557"
     CDS             complement(2711..4726)
                     /gene="tmcA"
                     /locus_tag="ACH3L1_001557"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFT4076345.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(4741..5604)
                     /locus_tag="ACH3L1_001558"
     CDS             complement(4741..5604)
                     /locus_tag="ACH3L1_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFT4076346.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5772..6485)
                     /gene="purC"
                     /locus_tag="ACH3L1_001559"
     CDS             complement(5772..6485)
                     /gene="purC"
                     /locus_tag="ACH3L1_001559"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFT4076347.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(6698..7732)
                     /gene="bamC"
                     /locus_tag="ACH3L1_001560"
     CDS             complement(6698..7732)
                     /gene="bamC"
                     /locus_tag="ACH3L1_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFT4076348.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(7749..8627)
                     /gene="dapA"
                     /locus_tag="ACH3L1_001561"
     CDS             complement(7749..8627)
                     /gene="dapA"
                     /locus_tag="ACH3L1_001561"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFT4076349.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            8773..9345
                     /locus_tag="ACH3L1_001562"
     CDS             8773..9345
                     /locus_tag="ACH3L1_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFT4076350.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            9345..9815
                     /gene="bcp"
                     /locus_tag="ACH3L1_001563"
     CDS             9345..9815
                     /gene="bcp"
                     /locus_tag="ACH3L1_001563"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFT4076351.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            10068..10685
                     /gene="hyfA"
                     /locus_tag="ACH3L1_001564"
     CDS             10068..10685
                     /gene="hyfA"
                     /locus_tag="ACH3L1_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFT4076352.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            10685..12702
                     /gene="hyfB"
                     /locus_tag="ACH3L1_001565"
                     /pseudo
     CDS             10685..12702
                     /gene="hyfB"
                     /locus_tag="ACH3L1_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            12713..13660
                     /gene="hyfC"
                     /locus_tag="ACH3L1_001566"
     CDS             12713..13660
                     /gene="hyfC"
                     /locus_tag="ACH3L1_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFT4076353.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            13677..15116
                     /gene="hyfD"
                     /locus_tag="ACH3L1_001567"
     CDS             13677..15116
                     /gene="hyfD"
                     /locus_tag="ACH3L1_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFT4076354.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            15128..15778
                     /gene="hyfE"
                     /locus_tag="ACH3L1_001568"
     CDS             15128..15778
                     /gene="hyfE"
                     /locus_tag="ACH3L1_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFT4076355.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            15783..17369
                     /gene="hyfF"
                     /locus_tag="ACH3L1_001569"
     CDS             15783..17369
                     /gene="hyfF"
                     /locus_tag="ACH3L1_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFT4076356.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            17359..19073
                     /gene="hyfG"
                     /locus_tag="ACH3L1_001570"
                     /pseudo
     CDS             17359..19073
                     /gene="hyfG"
                     /locus_tag="ACH3L1_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            19083..19628
                     /gene="hyfH"
                     /locus_tag="ACH3L1_001571"
     CDS             19083..19628
                     /gene="hyfH"
                     /locus_tag="ACH3L1_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFT4076357.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            19625..20383
                     /gene="hyfI"
                     /locus_tag="ACH3L1_001572"
     CDS             19625..20383
                     /gene="hyfI"
                     /locus_tag="ACH3L1_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFT4076358.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            20376..20789
                     /gene="hyfJ"
                     /locus_tag="ACH3L1_001573"
     CDS             20376..20789
                     /gene="hyfJ"
                     /locus_tag="ACH3L1_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFT4076359.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            20819..22831
                     /gene="hyfR"
                     /locus_tag="ACH3L1_001574"
                     /pseudo
     CDS             20819..22831
                     /gene="hyfR"
                     /locus_tag="ACH3L1_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            22853..23725
                     /gene="focB"
                     /locus_tag="ACH3L1_001575"
                     /pseudo
     CDS             22853..23725
                     /gene="focB"
                     /locus_tag="ACH3L1_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(23819..24880)
                     /locus_tag="ACH3L1_001576"
     CDS             complement(23819..24880)
                     /locus_tag="ACH3L1_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFT4076360.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            25093..26556
                     /gene="bepA"
                     /locus_tag="ACH3L1_001577"
     CDS             25093..26556
                     /gene="bepA"
                     /locus_tag="ACH3L1_001577"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFT4076361.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            26577..26936
                     /gene="arsC"
                     /locus_tag="ACH3L1_001578"
     CDS             26577..26936
                     /gene="arsC"
                     /locus_tag="ACH3L1_001578"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFT4076362.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(27069..27770)
                     /locus_tag="ACH3L1_001579"
     CDS             complement(27069..27770)
                     /locus_tag="ACH3L1_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFT4076363.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(27865..29154)
                     /gene="uraA"
                     /locus_tag="ACH3L1_001580"
     CDS             complement(27865..29154)
                     /gene="uraA"
                     /locus_tag="ACH3L1_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFT4076364.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(29240..29866)
                     /gene="upp"
                     /locus_tag="ACH3L1_001581"
     CDS             complement(29240..29866)
                     /gene="upp"
                     /locus_tag="ACH3L1_001581"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFT4076365.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            30190..31227
                     /gene="purM"
                     /locus_tag="ACH3L1_001582"
     CDS             30190..31227
                     /gene="purM"
                     /locus_tag="ACH3L1_001582"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFT4076366.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            31227..31865
                     /gene="purN"
                     /locus_tag="ACH3L1_001583"
     CDS             31227..31865
                     /gene="purN"
                     /locus_tag="ACH3L1_001583"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFT4076367.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            32037..34103
                     /gene="ppk1"
                     /locus_tag="ACH3L1_001584"
     CDS             32037..34103
                     /gene="ppk1"
                     /locus_tag="ACH3L1_001584"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFT4076368.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            34108..35649
                     /gene="ppx"
                     /locus_tag="ACH3L1_001585"
     CDS             34108..35649
                     /gene="ppx"
                     /locus_tag="ACH3L1_001585"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFT4076369.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(35688..>36796)
                     /gene="pdeF"
                     /locus_tag="ACH3L1_001586"
     CDS             complement(35688..>36796)
                     /gene="pdeF"
                     /locus_tag="ACH3L1_001586"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFT4076370.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         8526 a         9722 c         9561 g         8987 t
ORIGIN      
        1 agttgttaat ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa
       61 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
      121 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
      181 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
      241 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
      301 gtaaactgga cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
      361 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
      421 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
      481 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
      541 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
      601 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct
      661 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
      721 ttgaacccac cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct
      781 cgctggcggc gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg
      841 ggcgactggc atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa
      901 aagtcgtcga ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
      961 cgtcgagtat cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
     1021 gcaacctcac cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc
     1081 cggtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
     1141 atgaattctt cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta
     1201 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
     1261 ctgatgagat gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata
     1321 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
     1381 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
     1441 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
     1501 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
     1561 cccgtatgta tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg
     1621 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
     1681 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
     1741 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
     1801 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
     1861 gttaacgccg catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca
     1921 ggccaggtaa tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc
     1981 acttagcgct tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa
     2041 ttgcatactg cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc
     2101 accaccggca ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg
     2161 atcttcacca ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct
     2221 cgcatagcgt ccgttaaagg caatgacgcg cgaagcaagg cccggttcgg ctttaatgct
     2281 ctccagcgcc ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac
     2341 tccgctctgt ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac
     2401 acgcgcctgg cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg
     2461 attaccgctc ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc
     2521 aaaccagctg cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag
     2581 taacaactgt tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc
     2641 gaactcctta actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa
     2701 attgaaggag tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc
     2761 gaacctcatt tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca
     2821 tcttgcggcc tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta
     2881 aacgcccacg cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac
     2941 ctaacgatgt taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt
     3001 cgtcagaaag gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg
     3061 cgagagcttg cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg
     3121 catcactcat cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat
     3181 tacccatccg caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt
     3241 aaccaaaact caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa
     3301 gttgtcgccc tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc
     3361 ttccgcgcaa tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca
     3421 gattaccccg cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc
     3481 cgccctcatc caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa
     3541 aatgttgccc tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg
     3601 caccagacaa taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt
     3661 gttcaaatgc ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa
     3721 aaaccagtgc ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct
     3781 gttgcagttc aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac
     3841 gtccggtacc ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata
     3901 ccagttgatg caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg
     3961 cttgctcatc gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa
     4021 attgtgccag tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa
     4081 tacgagaaat gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg
     4141 ctgccacgcc cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg
     4201 gtgcgccggt cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct
     4261 ggttttgccg ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga
     4321 caaaatgcgg cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag
     4381 gttggttttc ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca
     4441 acgttccgct aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg
     4501 catgccggaa ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag
     4561 catctagctg cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag
     4621 catgatcaaa acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac
     4681 gtttcatttg cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa
     4741 ttaaatgctt ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt
     4801 aaaccagctg tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc
     4861 ctgactttgc tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc
     4921 cagatcgcca gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa
     4981 acagtcggcc tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt
     5041 ttgttgcagt tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac
     5101 ttcatgggcg ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat
     5161 gtcatcatag aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc
     5221 cataatggac tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt
     5281 cggttgctga taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt
     5341 tgcctgaatc accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt
     5401 tgattgttgt tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta
     5461 gcctgcaacc agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa
     5521 accgggaccg cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact
     5581 ttcacgtcgc ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc
     5641 gtaggtggtt cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc
     5701 gttcaatgca tatattccaa gagcggttgt cttgccgtgc cagctgcacg gcaagatgat
     5761 gaacagaaaa atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg
     5821 atcaggccac cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg
     5881 tcccacaggc ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac
     5941 agaccaaact ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc
     6001 agcacgtcgt tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc
     6061 cagccaaagg tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc
     6121 aggaacagat cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc
     6181 actagagagc cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt
     6241 ttcaccagac attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca
     6301 gccagtttgc tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac
     6361 tgctcaatgc gcgcgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg
     6421 tccgggtttt ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt
     6481 tgcatcttta tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg
     6541 ccggatgttt tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat
     6601 ttcgtgcgaa ttaccgctaa aatcgcgttt tcctgccgac gcacacgttt gcgtatcata
     6661 tcagaaaaaa gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga
     6721 agacagctac cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga
     6781 actgtaggct gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca
     6841 ggcctggatc gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta
     6901 cggccatgtt gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg
     6961 ccgctggcag acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac
     7021 cggtgtcatc agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt
     7081 tcgcggcgtc agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc
     7141 tgtaacgctg catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca
     7201 gtttaaccgt aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac
     7261 gatactgctc gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac
     7321 cagcatcatc acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg
     7381 gccacagagt attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact
     7441 gggtacgcgc gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct
     7501 taccgacagc accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca
     7561 aaatcattcc agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat
     7621 caccactgac ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa
     7681 gcgaaacacc cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc
     7741 cctaaacttt acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg
     7801 tcggtgattg gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta
     7861 catgcccatt tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc
     7921 agacgctgat taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc
     7981 tgggccatat cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat
     8041 tgcatgaagt ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc
     8101 agctctttga tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata
     8161 ttttttactt tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac
     8221 ggcacattat acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga
     8281 tacaaacctt cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata
     8341 ccactgtcat tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc
     8401 ccggcaatta ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca
     8461 tgttcgtcat gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa
     8521 gtaccgctgg cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct
     8581 ttttcatcca tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg
     8641 tgcaaacaag tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg
     8701 ttctgattgt tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa
     8761 ggaataacca gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc
     8821 gatcgccctg gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt
     8881 gaagacagtc gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt
     8941 tcatggaatg ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat
     9001 cttttaatcg tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc
     9061 tgggttcagg tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc
     9121 gacgcgcatc atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa
     9181 cgggctgcgc agttgcatat tcagataacc gcccacagcc ccgcatctgc ggacgcagca
     9241 aatattgagc aagcgttcaa agccctatgt acagaactca atgcacaagg cagtattaac
     9301 gtcgtcaatt attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag
     9361 ccggtgatat cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga
     9421 ccgacttcca gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct
     9481 gtaccgtaca ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg
     9541 atgtgctggg tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc
     9601 tgcttaactt tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct
     9661 ggggtgaaaa gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga
     9721 ttgacgctga tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg
     9781 acgttgtgct gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc
     9841 tgcgcttgcg gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct
     9901 gcgacacggg tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt
     9961 tcagaatcca gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt
    10021 tatacaatat gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg
    10081 tggtggccga accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg
    10141 tgcataaaac gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa
    10201 tcactgcccc tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg
    10261 tcaatgccat ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct
    10321 gcaagctttg cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg
    10381 tgaatgccca tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa
    10441 acgtgccgcc ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga
    10501 aatgcgacct gtgtgatttc ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc
    10561 aggcgttacg gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc
    10621 ttgccgcaag ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac
    10681 actaatggat gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct
    10741 ggcttcgctg tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc
    10801 gctggtgggc ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac
    10861 tttagtcgcc cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag
    10921 cctctcggca tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc
    10981 attgacttat atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa
    11041 tcttttcatc gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt
    11101 gctgtttgaa atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac
    11161 gtcgatcaac gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat
    11221 gatcgccttc ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg
    11281 ctttcacttt ctccggggct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc
    11341 gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca
    11401 tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg
    11461 aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg
    11521 atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat
    11581 atcaaacggc tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc
    11641 ggtgtggcga tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc
    11701 ggcgcactgt ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg
    11761 gacgcgatta tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa
    11821 cggatgccgt ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct
    11881 ccgctgaatg gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt
    11941 gtggaagccg tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact
    12001 ggtgggctgg cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg
    12061 cgcagtacac acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta
    12121 ctgctcgcgg cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata
    12181 atgcatattg cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca
    12241 cttgtacccg gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg
    12301 gcgatgcctt tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt
    12361 cgtcgcacag gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca
    12421 ggcaatggcg tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa
    12481 cagctcgacc ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag
    12541 agcacagaac ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg
    12601 ctggtcaaag aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg
    12661 gtcgccgcac tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca
    12721 aactctttgc gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat
    12781 gctaacccca ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg
    12841 cgggccgggg atctggcagg attatcgcga tatccacaaa ctgtttaaac gccaggaagt
    12901 tgcgccgaca tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat
    12961 gctggtgctg gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg
    13021 cgatctgatc acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg
    13081 gctggatacc ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat
    13141 tctggtcgaa ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac
    13201 gcatatcgag atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt
    13261 actggcgtta ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga
    13321 tgttgctgaa gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg
    13381 gctggcgcta gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt
    13441 ggccctgttt ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc
    13501 acttgtcgtt acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa
    13561 cacgctggca cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct
    13621 tgctgcgctt gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg
    13681 aaaatcttgc tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt
    13741 cgccacaacg tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca
    13801 tgttgtcgct gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg
    13861 tcaacgtggg ggatgtggca ttatttggcc tggtcattga tcgcgtgagt acgctgattc
    13921 tgtttgtggt ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg
    13981 ataaaaatcg cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt
    14041 ttatcggcgc gatggcggga ctggtactct cctcgacgct gctcggtcag ttgttgtttt
    14101 ttgaaattac gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc
    14161 agcgttcagc actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg
    14221 ccgccgccac gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac
    14281 atggcgacgc acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg
    14341 cccagctacc gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg
    14401 cctatctcca cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta
    14461 tcgacggcgg caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca
    14521 ccattcttta tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct
    14581 ggtcgaccat cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct
    14641 cgcggctggc gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc
    14701 tgtttttcct tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc
    14761 tgcgtggcgt attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg
    14821 caattaccgg tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg
    14881 gttttgcgtt gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg
    14941 aatcggtcgc cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac
    15001 cgagcgaggc cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg
    15061 tactgattgt gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag
    15121 atgatgaatg accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc
    15181 gctgtttgtg attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc
    15241 gctggtgctg gtgtctattt tcgccactct ctcgtgcctg ttcgccacag agcaactgct
    15301 gatctggtcc gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta
    15361 cgctgcacga aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat
    15421 ggcactgctc gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct
    15481 accgatggct accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg
    15541 cctgctgtgc attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat
    15601 ggaaaacggc tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga
    15661 aataggtatc gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa
    15721 aatatggcgt acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg
    15781 agatgagtta ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc
    15841 tctgttttgc ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata
    15901 gcttagggat cacactgctg ctgattctag cactctgggt ggtccaaact gccgctgatg
    15961 caggagaaat attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc
    16021 tcgccattct tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc
    16081 atgaagtggc acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc
    16141 atctgttttt gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg
    16201 cggcgatcga agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt
    16261 catcgctgga atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc
    16321 tgttcggtac cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg
    16381 cgatattctg gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt
    16441 tggcctttgt gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg
    16501 cctggctgcc ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg
    16561 tattgctgaa ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca
    16621 tcggcagcga tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg
    16681 cggcattttt cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg
    16741 agaacatggg gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg
    16801 cgctgctgca caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca
    16861 atgtactgct caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca
    16921 tgccatttac cgccgtgctg tttggcggcg gtgcgctggc gctggcgctg gcagggatgc
    16981 cgcccttcaa catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc
    17041 acctgctgat tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac
    17101 ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg
    17161 gctggttgac cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc
    17221 atattccaca acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga
    17281 cgcacgacct gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat
    17341 ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc
    17401 gccgccctga aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa
    17461 caggtcacca ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat
    17521 caacatgatg gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat
    17581 tacgcggttt attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag
    17641 gcactggtcg atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg
    17701 gtctggggcg agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat
    17761 cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat
    17821 gcgatggatt atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc
    17881 aatgagggca acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat
    17941 gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc
    18001 ctgttttatg tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa
    18061 gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat
    18121 accaactcgg ttgaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc
    18181 tcgattctgc tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc
    18241 cattttgttg gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg
    18301 acgatggcgg aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt
    18361 gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg
    18421 cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc
    18481 actcagggca ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg
    18541 ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg
    18601 gcaatattcc aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg
    18661 tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc
    18721 cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct
    18781 ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat
    18841 tgttccgctg gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc
    18901 tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact
    18961 cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt
    19021 ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga
    19081 agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct
    19141 tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat
    19201 gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg
    19261 acgaccagca aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac
    19321 gttgtgaaga agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg
    19381 tcacccataa agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt
    19441 gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc
    19501 aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt
    19561 gcaaacaacg cgcaacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc
    19621 agctatgagt ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac
    19681 gcaaaagatg aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat
    19741 cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt
    19801 cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt
    19861 atttactggc gcagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc
    19921 ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca
    19981 cgatctctac agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc
    20041 cggctgcccg ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct
    20101 gcaacagaag attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt
    20161 gtggccgcag atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc
    20221 gggctatcgt caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc
    20281 tacaggaaat cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat
    20341 cgttcagcaa ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg
    20401 ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct
    20461 ctcaggtaat gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga
    20521 atgtcgcctt ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg
    20581 agcaagcccg acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca
    20641 gccacaccgc cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt
    20701 ggcaagcgcg tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta
    20761 tctatctgat ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat
    20821 ggctatgtca gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa
    20881 cggaatgctc gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt
    20941 catcccgctg ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag
    21001 caacttctat taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca
    21061 gggcgagatg gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga
    21121 gctacgcttt attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa
    21181 ctggctggca cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca
    21241 ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac
    21301 taatgcagtg ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat
    21361 ccatcatttt ttcggtctgg cttcagtcag tatggtactg ggcgatcatc gaaagaacga
    21421 gaagttcagc ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa
    21481 tatgcctggc gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac
    21541 gcaccgtgca gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc
    21601 taacggctgc gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc
    21661 attgttgctg gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca
    21721 acacatagcc gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac
    21781 cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa
    21841 tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt
    21901 agatattgtg gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa
    21961 agaggtgatc gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa
    22021 aatcaactgc gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa
    22081 aggggcgttt actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg
    22141 cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg
    22201 cgtattgcag gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt
    22261 cagagtcatt gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg
    22321 cagcgatctc ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg
    22381 tccggaagat atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa
    22441 tcgttcaatt gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc
    22501 gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa
    22561 cagtctgaat ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga
    22621 ttcagcgctt cgcagttcaa tggcgtagtt gctgcacccg acgacgccag agaatgacga
    22681 agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc
    22741 ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg
    22801 tctgggaatc tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa
    22861 caaactctct ttcgacttac agttgagcgc cagaaaagcg gcaatcgctg aacggattgc
    22921 cgcccataaa attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt
    22981 gtttatggcg atgtccgctg gcgtgtttat ggcgatcgga tttacttttt acctttccgt
    23041 tatcgccgat gccccgtctt cataggcatt aacccatctg gtgggcggcc tttgctttac
    23101 acttggcttt attttgctgg cggtttgcgg caccagcctg ttcacctcgt cggtaatgac
    23161 ggtaatggca aaaagtcggg gcgttattag ttggcgaact tggctgatta acgcacttct
    23221 ggtggcctgc ggtaatctgg caggtattgc ctgtttcagt ttgttaatct ggttttccgg
    23281 gctggtgatg agtgaaaacg cgatgtgggg agtcgcgatt ttacactgcg ccgagggcaa
    23341 aatgcatcat acatttactg aatctgtcag cctcggcatt atgtgcaatc tgatggtttg
    23401 cctggcgctg tggatgagtt attgcggacg ttcgttatgc gacaaaatcg tcgccatgat
    23461 tttgtccatc accctgtttg tcgccagtgg ctttgagcac tgtatcgcca atttgtttgt
    23521 gattccgttc gccattgcca ttcgccattt cgcccctacc tccttctggc aactggcgca
    23581 cagtagcgca gacaattttc cggcactgac ggtcagccat tttattaccg ccaatctgct
    23641 cccggtgatg ctgggtaata ttatcggcgg tgcagtgctg gtgagtattt gttatcgggc
    23701 tatttattta cgtcaggaat cctgaaaaat cagcccggcg aaacagttcg tcgggctatt
    23761 tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt
    23821 attcttgcgc gatttgccca tcgggccagg cgtggatcac ggctttgatc agcgtcgcca
    23881 atggaatggc gaagaatacg ccccagaatc cccacaaacc accgaagatc accaccgata
    23941 aaataatcac cagcggatgc aggttaaccg cttcggagaa caacaccggt actagcaggt
    24001 tgccgtccag cgcctgaata atcagataca ccgcgaagca gctccagaat tcagtacctg
    24061 cgccaaactg gaatagcgcc acgccaacca ccggaatggt caccacaaat gcgccaatgt
    24121 acggaataag aaccgagaag ccgaccagca ctgccagcag cagcgaatag ttcagcccga
    24181 agagcaagaa ccccagccag gtggcgatcc ccaccacgat catctccagc actttgccgc
    24241 ggatatagtt ggtgatttgt tgattcatct ccttccacac ctgtcctgcc agcccacggt
    24301 tacgcggcag cacccggcga acggcgttca gcatctgctc tttgtctttc agcaggaaga
    24361 agaccatcaa tggcaccagc accagataga cggctatggt cagcaaaccg accagcgagg
    24421 cgagggaaat tttcaccacc gaatcgccca tggtcagcat ccgactgcgc atattttcgg
    24481 ccattgcatc aataatgccc gcgtccatta acaccggata gcggcgcggc aacgtggcgg
    24541 caaagtcaga aagcttattg agcatccccg gcatatcgcg gattaagtag atgccctgtt
    24601 gccaggcgat aggcagtacc acgaacgcca tcagtagcaa tataccgaca aaaaccacca
    24661 atacaatcga cgtcgcccag cggcgggagc agccaataga ttgcaggcgc acggttggcc
    24721 attccagcaa ataagccagc acaatagcca ccagcaacgg agcaagcagg ccactaaaga
    24781 aaaagataat gccaaatccg gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc
    24841 taaaacggcg gcgataccat tgcatcaaca tttcgagcat aaagaccttc cctgaacctc
    24901 aagagcggga ttgcgatccg caattgtatc gaaatgtcac aaaaaagact tcgcttttta
    24961 tgacggattc aggaaactga aaagtcattt gagtgggcta atcttcgccg ttacactcaa
    25021 aggcggcgag gtgggaacga tatttcacag tatcggtcaa atgactatct tcagaaatac
    25081 aggatagagg ttatgttcag gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct
    25141 atgaccattg gtcaggtagc cccggcgttt gccgacagcg cagacacctt gccggatatg
    25201 ggaacctccg caggaagcac gctttccatt ggtcaggaaa tgcagatggg cgactattat
    25261 gtccgccagc tacgcggcag cgcgccgtta attaatgacc cgctgttaac gcaatatatt
    25321 aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg ttaagacacc gtttcatttt
    25381 tttctgatca acaacgacga aattaacgcc tttgctttct ttggcggcaa cgtggtgctg
    25441 cactctgccc tgttccgtta ttccgataac gaaagtcaac tggcttcagt tatggcgcac
    25501 gaaatctccc acgtcaccca acgtcacctg gcgcgagcga tggaagatca acaacgcagc
    25561 gcgccgctga cctgggtcgg cgcgttaggt tctattttac tggcgatggc cagtccgcag
    25621 gcggggatgg cggcgctgac cggtacactg gcgggaacgc gccaggggat gatcagtttc
    25681 acccagcaaa atgaacagga agcggaccgc attggtattc aggtgctgca acgctcggga
    25741 ttcgatccac aggcgatgcc gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc
    25801 tcgcgcccgc cggaaatttt attgactcac ccgttgccgg aaagtcgtct ggcagatgcc
    25861 cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg
    25921 gcgaaagcgc gcacactggg gatgtataat tccggacgta accagctcac cagtgatttg
    25981 ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct
    26041 ttacaggcga tggaagccaa taaatacgac gaggcgcgta aaacgctgca accgttactg
    26101 gcggcagaac ctggcaacgc atggtatctc gatctggcta ccgatatcga tcttgggcaa
    26161 aacaaagcca atgaggcaat caatcgcctg aaaaatgccc gcgatttacg caccaatccg
    26221 gtgttgcagc tcaacctggc gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc
    26281 aatattctga atcgctacac ctttaataat aaagatgaca gcaacggctg ggatttgctg
    26341 gcacaggcgg aagccgcgct aaataaccgc gatcaggagc tggctgcgcg agcagaaggt
    26401 tatgcgctcg ccggacgact cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag
    26461 gtgaaattag gcagcctgca acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag
    26521 ctgcagaaac gctttaagcc ttataccaag atgtaattcg cattaaagga agaacaatga
    26581 ccaaacaggt aaaaatctac cataacccac gctgttcaaa gagccgggaa acgctgaacc
    26641 tgctgaaaga aaacggcgtg gagccagaag tggtgctcta ccttgagaca cccgccgatg
    26701 cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc
    26761 gccagaaaga ggatctttat aaagaactaa acctggctga cagttcgctt agcgaagagg
    26821 cgctgattca ggcaatggtg gataatccga agctgatgga gcgcccgatt atggtcgcga
    26881 atggcaaagc gcggattggt cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg
    26941 tgctccatcg ctagttgaag cacattgccg gatgcgacgc ttgccacgtc ttatccggcc
    27001 tacgaatact atggattaac ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa
    27061 caccttatct acaacttcag aatttctttc acaaacggaa tggtcagctt acgttgcgcg
    27121 gtaatcgacg cacgatccaa ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg
    27181 agccgcttca gcaagaaacg ccccacatct tccggcagtt caaaaccacg caaacgcgcg
    27241 cgtaactgta gcgcctgcaa cttatcttca tcagaaagtg gctgcaattt gtagatctgc
    27301 ccccagtcga gacgcgacgc gagatccggt aatcccagat tcaactgccg cggtggacga
    27361 tcgccggtga tcaacaaccg tgttttgccc gattccagaa ttcgattgta gagatcgaaa
    27421 atcgccatct cccacaactc atcgcctgca atacactcaa tgttgtcgat acagaccagc
    27481 gacaaatgct ccataccgtc gagcacttcc ggaacaaacc aggtgcgttt atccagcggg
    27541 acatagccca ccgcatcgcc acgctgcgac aattccgcgc aagccgcgtg cagcagatgg
    27601 ctgcgccccg cgccttcgcg tgcccagaga tagatgtaac cgctatgttc ctgacgcagc
    27661 acgttttgca gcgcggccag taaagaggag ttatcccccg gccagaaact tgcaaaggtt
    27721 tcgtcgtcag gaagataaag tggcaaagag agctgtgccg gtgtgttcag agatacctca
    27781 accaggattt cacaaaatcg cgagaagttt accacagaat cccatgatgt ttgaaccggg
    27841 cagcaacact gcccggtcgg tacattattt gtctgttata tccgcgtctt ctgcgtccag
    27901 caccacttct tcagggcgca gcacactgat cagtttgaaa atcaaactta agccgatacc
    27961 gacgatggtc gccagcgcca taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc
    28021 actgacgccg atgatcaaaa tcacggaagt caggatcagg ttctgtgctt tgttgtaatc
    28081 cactttcgat tcgatcaaaa cacgaatacc ggaagcacca atgacaccat aaagcagcag
    28141 cgaaacgccg cccatcaccg gcaatgggat catctggata gcggcagcca gtttaccgac
    28201 gcaggaaagc aggatagcga aaatcgccgc cccgccgata acccaggtac tgtaaacacg
    28261 ggtgatcgcc atcacgccaa tgttttctcc gtaagtggta tttggcgtag agccaaagaa
    28321 gccggagatc acggtcgaca ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg
    28381 cagcagatct tttttgacga tattagccgt tactaccagg tgccctacgt gttcggcaat
    28441 aaccactaac gccgctggca gaatagtcag aatggcaaac cactcgaagc gcggcgtata
    28501 gagggttggc agtgcaaacc agtgagcatt aataatcggc gtggtatcga caattcccat
    28561 tgcgaaagag agcgcgtacc ccaccagcac gccaattaaa atcgggataa ttgccaggaa
    28621 accacgaaac agcacggaac ctaaaaccgt gaccgccagg gtggtaatag agatgatgat
    28681 ggttttggag tttggcgttt gcccttcagc cgggagtaaa cccgccatac cggcagctac
    28741 gcccgccagc tccagaccga tgacggcaac gattgcgccc attgccgcag gtggaaacag
    28801 cacgtccaac cagccggtcc ccgctttctt cacgataaaa gaaaccaggc agaacagcac
    28861 gccgcacata ataaagccgc ccagcgcgac ttcataccct aacggcaaca gtaacaatac
    28921 cggtgaaata aaggcaaagc tggaaccaag ataagccgga attttccctt tacagatgaa
    28981 gagatacagc agcgttccaa taccgttaaa taacagtaca gtcgccgggt taatatgaaa
    29041 taagacgggc accaggacgg ttgcaccaaa catggcgaac aaatgttgca aactaagcgg
    29101 gattgtctgt aaaagtggcg gtctttcact caccccgata gcacggcgcg tcatagtatt
    29161 atcctctgta ttatgtgtta taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg
    29221 ctttaattat ttttattctt tatttcgtac caaagatttt gtcaccggca tcgccgaggc
    29281 ccggaataat gtatccgtgc tcgttcagtc cctggtcaat cgatgcagta tacagttcga
    29341 cgtccgggtg cgctttttcc agcgcagcga taccttctgg cgcagcgacc agcaccagaa
    29401 ctttgatgct gctgcagccc gcttttttca gcaggtcgat ggtcgcgata acggaaccac
    29461 cggttgccag cattgggtca acgatcagcg ccatacgctc atcgatgtta gaaaccagtt
    29521 tctggaagta cggtaccggc tccagcgttt cttcattacg gtacataccg acaacgctga
    29581 tgcgcgcgct cggaacgttt tccagcacac cgtccatcat accaagaccc gcacgcagaa
    29641 ttggcacaac ggtaattttc ttacctttga tctggtcgat ttctaccggg ccgttccagc
    29701 cttcgatagt tactttttcc gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac
    29761 ccacttcgga agcgagttcg cgaaagcgct tggtgctgat atcttgctca cgcatcagtc
    29821 ccagcttgtg tttgacgagt gggtgtttga cttccacgat cttcatactc tttctccttt
    29881 gaggggcagc cacaaaaaaa tcgacggatt atacctcctt tcttcagggc ggcaatatta
    29941 ttttcgttga ctttagtcaa aatgataaca gtttgagata aagttatttt atattcaaat
    30001 ggttatgaaa gaagattatt ccatccgaaa actaaccttt gccctggcac aagtcttctt
    30061 tcgccgcgcg cccggggaaa agacgtgcaa aaaggttgtg taaaacagtc tcgcaaacgt
    30121 ttgctttccc tgttagaatt gcgccgaatt ttatttttct accgcaagta acgcgtgggg
    30181 acccaagcag tgaccgataa aacctctctt agctacaaag atgccggtgt tgatattgac
    30241 gcgggtaatg ctctggttgg aagaatcaaa ggcgtagtga agaaaacgcg tcgtccggaa
    30301 gtgatgggcg gtctgggcgg cttcggtgcg ctgtgtgcat tgccgcaaaa atatcgtgaa
    30361 cccgtgctgg tttccggcac tgacggcgta ggtaccaagc tgcgtctggc aatggactta
    30421 aaacgtcacg acaccattgg tattgatctg gtcgccatgt gcgttaatga cctggtggtg
    30481 caaggtgcgg aaccgctgtt tttcctcgac tattacgcaa ccggaaaact ggatgttgat
    30541 accgcttcag cggtgatcag cggcatcgcg gaaggttgtc tgcaatcggg ctgttctctg
    30601 gtgggtggcg aaacggcaga aatgccgggg atgtatcacg gtgaagatta cgatgtcgcg
    30661 ggtttctgcg tgggcgtggt agaaaaatca gaaatcatcg acggctctaa agtcagcgac
    30721 ggcgatgtgc tgattgcact cggttccagc ggtccgcact cgaacggcta ttcgctggtg
    30781 cgcaaaattc ttgaagtcag cggttgtgat ccgcaaacca ccgaacttga tggtaagcca
    30841 ttagccgatc atctgctggc accgacccgc atttacgtga agtcagtgct ggagttgatt
    30901 gaaaaggtcg atgtgcatgc cattgcgcac ctgaccggcg gcggcttctg ggaaaacatt
    30961 ccgcgcgtat tgccagataa tacccaagca gtgattgatg aatcttcctg gcagtggccg
    31021 gaagtgttca actggctgca aacggcaggt aacgttgagc gccatgaaat gtatcgcacc
    31081 ttcaactgcg gcgtcgggat gattatcgcc ctgcctgctc cggaagtgga caaagccctc
    31141 gccctgctaa atgccaacgg tgaaaacgcg tggaaaatcg gtatcatcaa agcctctgat
    31201 tccgaacaac gcgtggttat cgaataatga atattgtggt gcttatttcc ggcaacggaa
    31261 gtaatttaca ggcaattatt gacgcctgta aaaccaacaa aattaaaggc accgtacggg
    31321 cagttttcag caataaggcc gacgcgttcg gccttgaacg cgcccgccag gcgggtattg
    31381 caacgcatac gctcatcgcc agcgcgtttg acagtcgtga agcctatgac cgggagttga
    31441 ttcatgaaat cgacatgtac gcacccgatg tggtcgtgct ggctggtttt atgcgcattc
    31501 tcagcccggc gtttgtctcc cactatgccg ggcgtttgct gaacattcac ccttctctgc
    31561 tgccgaaata tcccggatta cacacccatc gtcaggcgct ggaaaatggc gatgaagagc
    31621 acggtacatc ggtgcatttc gtcaccgatg aactggacgg tggcccggtt attttacagg
    31681 cgaaagtccc ggtatttgct ggtgatacgg aagatgacat caccgcccgc gtgcaaaccc
    31741 aggaacacgc tatttatcca ctggtgatta gctggtttgc cgatggtcgt ctgaaaatgc
    31801 acgaaaacgc cgcgtggctg gatggtcaac gtctgccgcc gcagggctac gctgccgacg
    31861 agtaatgccc ccgtagttaa agcgccagct ctgccgctgg cgtttttcaa ttcacctgta
    31921 aatcgcaagc tccagcagtt tttttccccc ttttctggca tagttggaca tctgccaata
    31981 ttgctcgcca taatatccag gcagtgtccc gtgaataaaa cggagtaaaa gtggtaatgg
    32041 gtcaggaaaa gctatacatc gaaaaagagc tcagttggtt atcgttcaat gaacgcgtgc
    32101 ttcaggaagc ggcggacaaa tctaacccgc tgattgaaag gatgcgtttc ctggggatct
    32161 attccaataa ccttgatgag ttctataaag tccgcttcgc tgaactgaag cgacgcatca
    32221 ttattagcga agaacaaggc tccaacgctc attcccgcca tttactgggc aaaattcagt
    32281 cccgggtgct gaaagccgat caggaattcg acggcctcta caacgagcta ttgctggaga
    32341 tggcgcgcaa ccagatcttc ctgattaatg aacgccagct ctccgtcaat caacaaaact
    32401 ggctgcgtca ttattttaag cagtatctgc gtcagcacat tacgccgatt ttaatcaatc
    32461 ctgacactga cttagtgcag ttcctgaaag atgattacac ctatctggcg gtggaaatta
    32521 tccgtagcga tagcatccgt tacgcgctgc tggagatccc atcagataaa gtgccgcgct
    32581 ttgtgaattt accgccagaa gcgccgcgtc gacgcaagcc gatgattctt ctggataaca
    32641 ttctgcgtta ctgccttgat gatattttca aaggcttctt tgattatgac gcgctgaatg
    32701 cctattcaat gaagatgacc cgcgatgccg aatacgattt agtgcatgag atggaagcca
    32761 gcctgatgga gttgatgtct tccagtctca agcagcgttt aactgctgag ccggtgcgtt
    32821 ttgtttatca gcgcgatatg cccaatgcgc tggttgaagt gttacgcgaa aaactgacta
    32881 tttcccgcta cgactccatc gtccccggcg gtcgttatca taattttaaa gactttatta
    32941 atttccccaa tgtcggcaaa gccaatctgg tgaacaaacc actgccgcgt ttacgccata
    33001 tttggtttga taaagcccag ttccgcaatg gttttgatgc cattcgcgaa cgcgatgtgt
    33061 tgctctatta tccttatcac acctttgagc atgtgctgga actgctgcgt caggcttcgt
    33121 tcgacccgag cgtactggcg attaaaatta acatttaccg cgtggcgaaa gattcacgca
    33181 tcatcgactc gatgatccac gccgcacata acggtaagaa agtcactgtg gtggttgagt
    33241 tacaggcgcg tttcgacgaa gaagccaaca ttcactgggc gaagcgcctg accgaagcag
    33301 gcgtgcacgt tatcttctct gcgccggggc tgaaaattca cgccaaactg ttcctgattt
    33361 cacgtaaaga aaacggtgaa gtggtccgtt acgcacacat cgggaccggg aactttaacg
    33421 aaaaaaccgc gcgtctttat actgactatt cgttgctgac cgcagatgcg cgtatcacca
    33481 acgaagtacg gcgggtattt aactttattg aaaacccata ccgcccggtg acatttgatt
    33541 atttaatggt gtcaccgcaa aactctcgcc gtctgttata tgaaatggta gaccgcgaaa
    33601 tcgccaacgc gcagcaaggg ctgcccagtg gtatcaccct gaagctaaat aaccttgtcg
    33661 ataaaggcct ggttgatcgt ctgtatgcgg cctccagctc cggcgtaccg gttaatctgc
    33721 tggttcgcgg aatgtgttcg ctgatcccca atctggaagg cattagcgac aacattcgtg
    33781 ccatcagtat tgttgaccgt taccttgaac atgaccgggt ttatattttt gaaaatggcg
    33841 gcgataaaaa ggtctacctt tcttccgccg actggatgac gcgcaatatt gattatcgta
    33901 ttgaagtggc gacaccgctg ctcgatccgc gcctgaagca gcgggtgctg gacatcatcg
    33961 acatattgtt cagcgatacg gtcaaagcac gttatatcga taaagaactc agtaatcgct
    34021 acgttccccg cggcaatcgc cgcaaagtac gggcgcagtt ggcgatttac gactacatca
    34081 aatcactcga acaacctgaa taaccctatg ccaatacacg ataaatcccc tcgtccgcag
    34141 gagtttgctg cggtcgatct tggttcaaac agttttcaca tggtcatagc ccgtgtggta
    34201 gatggtgcca tgcagattat tggccgcctg aaacagcggg tgcatctggc ggacggcctg
    34261 gggccagata atatgttgag tgaagaggca atgacgcgcg gtttgaactg tctgtcgctg
    34321 tttgccgaac ggctacaagg gttttctcct gccagcgtct gtatagtcgg cacccatacg
    34381 ctgcgtcagg cgctgaacgc cactgacttt ctgaaacgcg cggaaaaggt cattccctac
    34441 ccgattgaaa ttatttccgg taatgaagaa gcccgtctga tttttatggg cgtggaacat
    34501 acccaaccgg aaaaaggtcg caaactggtt attgatattg gcggcggatc tacggaactg
    34561 gtgattggtg aaaatttcga acctattctc gttgaaagcc gccggatggg ttgtgtcagc
    34621 tttgcccagc tttattttcc tggcggggtc atcaataaag agaattttca gcgcgctcgc
    34681 atggcggcag cacaaaaact ggaaacttta acctggcaat tccgtattca gggctggaac
    34741 gttgcaatgg gcgcttccgg taccataaaa gccgcccatg aagtgttaat ggaaatgggc
    34801 gagaaagacg ggataattac cccggaacgt ctggaaaaac tggtaaaaga aattttacgt
    34861 caccgtaatt tcgcatcgct gagtttaccg ggtctttccg aagagcggaa aacagtcttc
    34921 gttcctggac tggcgatttt atgtggtgtg tttgatgctt tagccatccg tgaactgcgc
    34981 ctttctgacg gggcgttacg cgaaggcgta ctgtatgaaa tggaaggacg tttccgtcat
    35041 caggatgtgc gtagtcgcac cgccagcagc ctcgccaacc agtatcacat agacagcgaa
    35101 caggcccgac gagtgctgga taccactatg caaatgtacg aacagtggcg ggaacagcaa
    35161 ccgaagctgg cgcatccgca actggaggcg ctactgcgat gggccgccat gctgcatgag
    35221 gtcgggttga atatcaacca cagcggtttg catcgccact ccgcttatat tctgcaaaac
    35281 agtgacttgc cgggttttaa tcaggaacag cagctgatga tggcgacact ggtgcgctat
    35341 caccgtaaag cgattaagct cgacgatctg ccgcgcttta ccttgtttaa gaagaaacag
    35401 ttcctgccac tgatacagct attgcgcctt ggcgtattac taaacaatca acgtcaggca
    35461 accaccacac cgccaacatt gacactgatt actgatgaca gtcactggac actgcgtttc
    35521 ccgcatgact ggtttagtca gaatgcgctg gtactgcttg atctggaaaa ggagcaagaa
    35581 tactgggaag gcgtggctgg ctggcggttg aaaattgaag aagaaagtac cccagaaatc
    35641 gccgcttaat gctttgcggg ccgacgagaa atgtcggccc gcattattca ggcactttcg
    35701 cgaatgggtt cgatttcatt cagcgtatca attaacggtt gcggcttacc aataagataa
    35761 ccctgcatat aatcgatccc caaagagagc accgcctcgc ggatctcttc gttttcaacg
    35821 tactctgcca ctacccgcat tttcttcatt cgcgccagat ggcaaatcga cgccacaatc
    35881 tgataatcca gactatttga cacaatattg cggataaagc tgccgtcaat tttaagcaga
    35941 tcggcattca catttttaag ccgtgcatag ctggcgtagc cggtgccgaa atcatcaatc
    36001 gcaatctggc agcctaattc ctgaagatgc tgcaaggtga tttgcgcctg cttaacattg
    36061 gtcagagcat tactttcggt gacttcaaaa ataagttgcc acgcttcaat ctgatattta
    36121 gccagcaact gactgacttc aacaggaaaa cgagcctgac ataccgaggt tggagacaga
    36181 ttaatagcaa aacggtgagc gggcatcttc gctctgtttt cagccataaa ttgcagcgta
    36241 tgctcaatga cccacatgtc gatactcgac gataaaccaa attcgtgcgc gaccggcaag
    36301 aagctaccgg ggccgatcag ttcatcattc tcacctttca tgcgcagaag aatttcatgg
    36361 taaacatcac cacgcatacc ggtaatcggc tgggccatca ggaaaaaatg gttgtgttcc
    36421 agcgcctgct gtagtcgatt catcatcgcg actttatctt tcaattcgcg ttgcaaatac
    36481 attgccccgc aacgctgcat attttccggg gcgttggtca cgatggaaag ttcggcgacc
    36541 gtatttagct ctcccagcag caggtagata tgattcactg gcgagcgcac atagcagtaa
    36601 ctgacgccaa tctgcggttg catcggcatc ccatcccaaa agaaacgaaa ttgcttgaga
    36661 tgactatcca gtgcggtaat gcgctcctgg tgcgattctg tattcagtcg cagcgcgaga
    36721 tcgttacccg aaagctgata aacatcttca cccggttcca gcaagggtga cagccagtga
    36781 gaaagttttt gcttgt
//
LOCUS       DAZXUL010000033        36587 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZXUL010000033 DAZXUL010000000
VERSION     DAZXUL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36587
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.033"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..190
                     /locus_tag="ACH3L1_001587"
     CDS             <1..190
                     /locus_tag="ACH3L1_001587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076371.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACH3L1_001588"
     CDS             complement(156..1391)
                     /locus_tag="ACH3L1_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFT4076372.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACH3L1_001589"
     tRNA            complement(1538..1614)
                     /locus_tag="ACH3L1_001589"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="ACH3L1_001590"
     tRNA            complement(1657..1743)
                     /locus_tag="ACH3L1_001590"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="ACH3L1_001591"
     tRNA            complement(1764..1839)
                     /locus_tag="ACH3L1_001591"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="ACH3L1_001592"
     tRNA            complement(1898..1974)
                     /locus_tag="ACH3L1_001592"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="ACH3L1_001593"
     CDS             complement(2077..3462)
                     /locus_tag="ACH3L1_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFT4076373.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACH3L1_001594"
     CDS             complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACH3L1_001594"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFT4076374.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACH3L1_001595"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACH3L1_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFT4076375.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACH3L1_001596"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACH3L1_001596"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFT4076376.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACH3L1_001597"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACH3L1_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFT4076377.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACH3L1_001598"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACH3L1_001598"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFT4076378.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACH3L1_001599"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACH3L1_001599"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFT4076379.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACH3L1_001600"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACH3L1_001600"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFT4076380.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACH3L1_001601"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACH3L1_001601"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFT4076381.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACH3L1_001602"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACH3L1_001602"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFT4076382.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACH3L1_001603"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACH3L1_001603"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFT4076383.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACH3L1_001604"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACH3L1_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFT4076384.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACH3L1_001605"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACH3L1_001605"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFT4076385.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACH3L1_001606"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACH3L1_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFT4076386.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACH3L1_001607"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACH3L1_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFT4076387.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACH3L1_001608"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACH3L1_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFT4076388.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="ACH3L1_001609"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="ACH3L1_001609"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFT4076389.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="ACH3L1_001610"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="ACH3L1_001610"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFT4076390.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACH3L1_001611"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACH3L1_001611"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFT4076391.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="ACH3L1_001612"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="ACH3L1_001612"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFT4076392.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACH3L1_001613"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACH3L1_001613"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFT4076393.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="ACH3L1_001614"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="ACH3L1_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFT4076394.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACH3L1_001615"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACH3L1_001615"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFT4076395.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACH3L1_001616"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACH3L1_001616"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFT4076396.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVICVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACH3L1_001617"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACH3L1_001617"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFT4076397.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACH3L1_001618"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACH3L1_001618"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFT4076398.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACH3L1_001619"
     CDS             complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACH3L1_001619"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFT4076399.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACH3L1_001620"
     CDS             complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACH3L1_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFT4076400.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACH3L1_001621"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACH3L1_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFT4076401.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="ACH3L1_001622"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="ACH3L1_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACH3L1_001623"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="ACH3L1_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFT4076402.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="ACH3L1_001624"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="ACH3L1_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFT4076403.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36398..36473)
                     /locus_tag="ACH3L1_001625"
     tRNA            complement(36398..36473)
                     /locus_tag="ACH3L1_001625"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="ACH3L1_001626"
     tRNA            complement(36482..36558)
                     /locus_tag="ACH3L1_001626"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8939 a        10012 c         9218 g         8418 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcggggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaca gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gaagtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggtttta
//
LOCUS       DAZXUL010000034        35772 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZXUL010000034 DAZXUL010000000
VERSION     DAZXUL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35772
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.034"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(178..318)
                     /gene="yjjY"
                     /locus_tag="ACH3L1_001627"
     CDS             complement(178..318)
                     /gene="yjjY"
                     /locus_tag="ACH3L1_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFT4076404.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            414..1130
                     /gene="arcA"
                     /locus_tag="ACH3L1_001628"
     CDS             414..1130
                     /gene="arcA"
                     /locus_tag="ACH3L1_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFT4076405.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1189..2541)
                     /gene="creD"
                     /locus_tag="ACH3L1_001629"
     CDS             complement(1189..2541)
                     /gene="creD"
                     /locus_tag="ACH3L1_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFT4076406.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2599..4023)
                     /gene="creC"
                     /locus_tag="ACH3L1_001630"
     CDS             complement(2599..4023)
                     /gene="creC"
                     /locus_tag="ACH3L1_001630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFT4076407.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(4023..4712)
                     /gene="creB"
                     /locus_tag="ACH3L1_001631"
     CDS             complement(4023..4712)
                     /gene="creB"
                     /locus_tag="ACH3L1_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFT4076408.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4725..5198)
                     /gene="creA"
                     /locus_tag="ACH3L1_001632"
     CDS             complement(4725..5198)
                     /gene="creA"
                     /locus_tag="ACH3L1_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFT4076409.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5409..6278
                     /gene="robA"
                     /locus_tag="ACH3L1_001633"
     CDS             5409..6278
                     /gene="robA"
                     /locus_tag="ACH3L1_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFT4076410.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6275..6922)
                     /gene="gpmB"
                     /locus_tag="ACH3L1_001634"
     CDS             complement(6275..6922)
                     /gene="gpmB"
                     /locus_tag="ACH3L1_001634"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFT4076411.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6974..7494
                     /gene="yjjX"
                     /locus_tag="ACH3L1_001635"
                     /pseudo
     CDS             6974..7494
                     /gene="yjjX"
                     /locus_tag="ACH3L1_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(7579..7905)
                     /gene="trpR"
                     /locus_tag="ACH3L1_001636"
     CDS             complement(7579..7905)
                     /gene="trpR"
                     /locus_tag="ACH3L1_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFT4076412.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7995..9932)
                     /gene="sltY"
                     /locus_tag="ACH3L1_001637"
     CDS             complement(7995..9932)
                     /gene="sltY"
                     /locus_tag="ACH3L1_001637"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFT4076413.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10143..11810
                     /gene="ettA"
                     /locus_tag="ACH3L1_001638"
     CDS             10143..11810
                     /gene="ettA"
                     /locus_tag="ACH3L1_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFT4076414.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11917..12084)
                     /gene="nadS"
                     /locus_tag="ACH3L1_001639"
     CDS             complement(11917..12084)
                     /gene="nadS"
                     /locus_tag="ACH3L1_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFT4076415.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(12118..13350)
                     /gene="nadR"
                     /locus_tag="ACH3L1_001640"
     CDS             complement(12118..13350)
                     /gene="nadR"
                     /locus_tag="ACH3L1_001640"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFT4076416.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(13371..14753)
                     /gene="radA"
                     /locus_tag="ACH3L1_001641"
     CDS             complement(13371..14753)
                     /gene="radA"
                     /locus_tag="ACH3L1_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFT4076417.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMAEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14802..15770)
                     /gene="serB"
                     /locus_tag="ACH3L1_001642"
     CDS             complement(14802..15770)
                     /gene="serB"
                     /locus_tag="ACH3L1_001642"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFT4076418.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15876..16520
                     /locus_tag="ACH3L1_001643"
     CDS             15876..16520
                     /locus_tag="ACH3L1_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFT4076419.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16548..17563
                     /gene="lplA"
                     /locus_tag="ACH3L1_001644"
                     /pseudo
     CDS             16548..17563
                     /gene="lplA"
                     /locus_tag="ACH3L1_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            17595..17858
                     /locus_tag="ACH3L1_001645"
     CDS             17595..17858
                     /locus_tag="ACH3L1_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4076420.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(18019..18738)
                     /gene="deoD"
                     /locus_tag="ACH3L1_001646"
     CDS             complement(18019..18738)
                     /gene="deoD"
                     /locus_tag="ACH3L1_001646"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFT4076421.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18795..20018)
                     /gene="deoB"
                     /locus_tag="ACH3L1_001647"
     CDS             complement(18795..20018)
                     /gene="deoB"
                     /locus_tag="ACH3L1_001647"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFT4076422.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20070..21392)
                     /gene="deoA"
                     /locus_tag="ACH3L1_001648"
     CDS             complement(20070..21392)
                     /gene="deoA"
                     /locus_tag="ACH3L1_001648"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFT4076423.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(21519..22298)
                     /gene="deoC"
                     /locus_tag="ACH3L1_001649"
     CDS             complement(21519..22298)
                     /gene="deoC"
                     /locus_tag="ACH3L1_001649"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFT4076424.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22556..24097
                     /locus_tag="ACH3L1_001650"
     CDS             22556..24097
                     /locus_tag="ACH3L1_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFT4076425.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            24069..24932
                     /locus_tag="ACH3L1_001651"
     CDS             24069..24932
                     /locus_tag="ACH3L1_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFT4076426.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(25146..25928)
                     /locus_tag="ACH3L1_001652"
     CDS             complement(25146..25928)
                     /locus_tag="ACH3L1_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFT4076427.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(25925..26998)
                     /locus_tag="ACH3L1_001653"
     CDS             complement(25925..26998)
                     /locus_tag="ACH3L1_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFT4076428.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27120..27281)
                     /locus_tag="ACH3L1_001654"
     CDS             complement(27120..27281)
                     /locus_tag="ACH3L1_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFT4076429.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27398..28003)
                     /gene="osmY"
                     /locus_tag="ACH3L1_001655"
     CDS             complement(27398..28003)
                     /gene="osmY"
                     /locus_tag="ACH3L1_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFT4076430.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28396..29985)
                     /gene="prfC"
                     /locus_tag="ACH3L1_001656"
     CDS             complement(28396..29985)
                     /gene="prfC"
                     /locus_tag="ACH3L1_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFT4076431.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30076..30753)
                     /gene="yjjG"
                     /locus_tag="ACH3L1_001657"
     CDS             complement(30076..30753)
                     /gene="yjjG"
                     /locus_tag="ACH3L1_001657"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFT4076432.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(30768..31214)
                     /gene="rimI"
                     /locus_tag="ACH3L1_001658"
     CDS             complement(30768..31214)
                     /gene="rimI"
                     /locus_tag="ACH3L1_001658"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFT4076433.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31183..31596)
                     /gene="holD"
                     /locus_tag="ACH3L1_001659"
     CDS             complement(31183..31596)
                     /gene="holD"
                     /locus_tag="ACH3L1_001659"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFT4076434.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            31699..32730
                     /gene="rsmC"
                     /locus_tag="ACH3L1_001660"
     CDS             31699..32730
                     /gene="rsmC"
                     /locus_tag="ACH3L1_001660"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFT4076435.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            33007..33093
                     /locus_tag="ACH3L1_001661"
     tRNA            33007..33093
                     /locus_tag="ACH3L1_001661"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33041..33043,aa:Leu,seq:cag)
     gene            33122..33208
                     /locus_tag="ACH3L1_001662"
     tRNA            33122..33208
                     /locus_tag="ACH3L1_001662"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33156..33158,aa:Leu,seq:cag)
     gene            33243..33329
                     /locus_tag="ACH3L1_001663"
     tRNA            33243..33329
                     /locus_tag="ACH3L1_001663"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33277..33279,aa:Leu,seq:cag)
     gene            complement(33368..33604)
                     /locus_tag="ACH3L1_001664"
     CDS             complement(33368..33604)
                     /locus_tag="ACH3L1_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFT4076436.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            33745..34533
                     /gene="fhuF"
                     /locus_tag="ACH3L1_001665"
     CDS             33745..34533
                     /gene="fhuF"
                     /locus_tag="ACH3L1_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFT4076437.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(34571..35248)
                     /gene="bglJ"
                     /locus_tag="ACH3L1_001666"
     CDS             complement(34571..35248)
                     /gene="bglJ"
                     /locus_tag="ACH3L1_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFT4076438.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(35206..>35772)
                     /locus_tag="ACH3L1_001667"
     CDS             complement(35206..>35772)
                     /locus_tag="ACH3L1_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4076439.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT         8508 a         9788 c         9051 g         8425 t
ORIGIN      
        1 aattggattc accacgttta ttagttgtat gatgcaacta gttggattat taaaataatg
       61 tgacgaaagc tagcatttag atacgatgat ttcatcaaac tgttaacgtg ctacaattga
      121 acttgatata tgtcaacgaa gcgtagtttt attgggtgtc cggcccctct tagcctgtta
      181 tgttgctgtt aaaatggtta ggatgacagc cgtttttgac actgtcgggt cctgagggaa
      241 agtacccacg accaagctaa tgatgttgtt gacgttgatg gaaagtgcat caagaacgca
      301 attacgtact ttagtcatgt tacgccgatc atgttaattt gcagcatgca tcaggcaggt
      361 cagggacttt tgtacttcct gtttcgattt agttggcaat ttaggtagca aacatgcaga
      421 ccccgcacat tcttatcgtt gaagacgagt tggtaacacg caacacgttg aaaagtattt
      481 tcgaagcgga aggctatgat gttttcgaag cgacagatgg cgcggaaatg catcagatcc
      541 tctctgaata tgacatcaac ctggtgatca tggatatcaa tctgccgggt aagaacggtc
      601 ttctgttagc gcgtgaactg cgcgagcagg cgaatgttgc gttgatgttc ctgactggcc
      661 gtgacaacga agtcgataaa attctcggcc tcgaaatcgg tgcagatgac tacatcacca
      721 aaccgttcaa cccgcgtgaa ctgacgattc gtgcacgcaa cctgctgtcc cgtaccatga
      781 atctgggtac tgtcagcgaa gaacgtcgta gcgttgaaag ctacaagttc aatggttggg
      841 aactggacat caacagccgt tcgttgatcg gccctgatgg cgagcagtac aagctgccgc
      901 gcagcgagtt ccgcgccatg cttcacttct gtgaaaaccc aggcaaaatt cagtctcgtg
      961 ctgaactgct gaagaaaatg accggccgtg agctgaaacc gcacgaccgt actgtagacg
     1021 tgacgatccg ccgtattcgt aaacatttcg aatctacgcc ggatacgccg gaaatcatcg
     1081 ccaccattca cggtgaaggt tatcgcttct gcggtgatct ggaagattaa tcggctttac
     1141 caccgtcaaa aaaacggcgc tttttagcgc cgtttttatt tttcaacctt atttccagat
     1201 acgtaattca tcgtccgttg taacttcttt actggctttc attttcggca gtgaaaacgc
     1261 ataccagtct atattacgag tcacaaacat catgccggcc agcgccacca ccagcacact
     1321 ggttcccaac aacagcgcgc tatcggcaga gttgagcagt ccccacatca ctccatccag
     1381 caacaacagc gcgagggtaa acaacatgct attgcgccag cctttcaata ccgcctgcaa
     1441 ataaataccg ttcattaacg ccccaatcag actggcgatt atccatgcca cggtaaaacc
     1501 gatatgttca gaaagcgcca gcaagagcaa ataaaacatc accaatgaaa gccccaccag
     1561 caaatattgc attgggtgta aacgttgcgc ggtgagcgtt tcaaaaacaa agaacgccat
     1621 aaaagtcagt gcaatcagca gaatggcgta cttagtcgcc cggtccgtta attggtattg
     1681 atcggctggc gtcgtcactg cgacgctaaa cgcggggaag ttttcccagc cggtatcatt
     1741 gcctgaagca aaacgctcac cgagattatt agcaaaccag ctgctttgcc attgcgcctg
     1801 aaaacctgac tcgctagctt cccgtttggc tggtagaaaa tcacctaaaa aactgggatg
     1861 cggccagttg ctggttaagg tcatttcgct attacgcccg gcaggcacca cagaaagatc
     1921 gccggtaccg cttaaattca gtgccatatt cagctgcagg ttctgcttcc gccagtcccc
     1981 ttcgggtaaa gggatatgca cgccctgccc gccttgctct aacccggtac cgggttcaat
     2041 ggtcagtgcc gttccgttaa cttcaggcgc tttcaccaca ccaataccac gcgcatcccc
     2101 gacgctaatc acaataaagg gcttgcctaa gatgatattt ggcgcgttga gctcgctaag
     2161 acgcgaaaca tcgaaatcgg cttttagcgt taaatcactg tgccagacct gaccggtata
     2221 aatccctatc ttgcgttctt ccacgttctg attgccatca accatcaatg actcaggtaa
     2281 ccaaaaatgg ataaaacttc gtttccgctc cacggtttta tcctcttcct gcaccgtata
     2341 aagctcggtc acaggaatag cgataagcgg cccaacgagt ttttgcggcc cgctggtact
     2401 ttggcgaatc gcatcttcca catcgctacg gtaatcggca cgttcgacaa tcacttgccg
     2461 aatcagcatt atcggaatca acaacagcaa tactgcacca aacaaggtgg tcattttcca
     2521 gaacaggggg gatttcaaca tagtcttctc ctttgcaatg gcagcaggat acgaaggcta
     2581 tgtgggaaga atttgaagct atgtgaagtg acggtgaagt cgtagcgagg ccagcacgcc
     2641 accttcctgc acgttgcgca gcgtgacttc gccgttaaac aaacgggcga cctcactgac
     2701 gaacgccaac cccagaccgc tgcttttttg cccatttgca cgcggcaaag agtaaaagcg
     2761 ttcaaaaata cgtgacagcg cgtagtcagg aataccacta ccggtatcca gcaccttaag
     2821 cgtgacgtgt tcctgatcca cttcggcgct tagcgttatg caaccgctct cgggagtaaa
     2881 atcgatggcg ttatccagta aattccccag cgcctgatcc agtaacgccg gttcagcagc
     2941 aacattaacc tcggtgggca taacatgcaa agtgatgttt ttttctgcca actgcacggt
     3001 gcgcgcttcg ctgacgcggc gaaataatgc cgccacatca acagcagtca gaacgacttc
     3061 ctgacgattc tccagtcttg cctggcgtag taacgtttcc accagcgcct gcattcgcgc
     3121 attttgtgtc agaatgttgt cagtaaaacg agccactact tcaggcggcg gaccttcgcg
     3181 taaaatttcc gccgcgccac gaatcgccgc cagtgggctt tttagctcat gggttaacgc
     3241 ataaacatac tgctcaatat agtttttccc ttccagcttc acgcgcatac tttccagcgc
     3301 ctgcgcgagt ttacgcagct cgctactacc gagatcggga agaggaacgg gcttattgtc
     3361 agtgacggaa tcagcatagc gagtgagccg ggcaatggag cggttgatcc accaaaccat
     3421 gcctgcgcca atcaccagtg caatccccaa caaaatggcg ctggcccata aaattcgccg
     3481 ctcgctgcgt ttaatgaccg gagccatcgc cgcgttcggt ttgcctacgc tcaaaacgcc
     3541 aataagccgc gagccgccca taatcggcgc ggcaacatac atcaccgaac tttcgggatc
     3601 ggcaggattt tgcaacgtgc tgcgcgcacc atactgacca cgcaacgtta gccagacgtc
     3661 attccagcgc gaataatcct gtccaacggc tttatttgcc gaatcgaaca atactttgcc
     3721 atgcgcatcg gtcatataga catgatattc attgcgcact ttgttaatgc caccaatatt
     3781 ggcgcgaaac gggcgatgtt gtagctgatt aaacgcctgc gccagttgcc catgcgttgg
     3841 gtccccagag agcaaatcgg gacgcgccag ctccgcaagt aacgttgccg tgtcgatcaa
     3901 cgtcccctcc gttgctcttc gcacgcccgg tttaacttct ttgacaaaaa tagccagtac
     3961 gaaccaggcc gccaccgcca ccagtaaaaa atagcccagc agcaaccgca tgccgatacg
     4021 cattacaagc ccctcaggct atatcccatg ccgcgatgag tattaatcgg tgaaagatcg
     4081 gggttgatgg cgcgcagctt ggcacgcagc gttttaatgt ggatatcgac ggtgcgatcg
     4141 taggtgtcct gcgcatcttc ccacacgcta tccattagtt gctggcggga ccagacgcgg
     4201 cctggcgact tgagtaacgt cttcagcaat aaaaactcat accgcgtcag cgttaatggc
     4261 gtgtcaaacc agctgatctg cgccgcgggt tcattcaatt caaaatgacc aatacggatg
     4321 acgggagacg gcgtcgagaa cttcttcacc cgacgcagta aggtgcgcac cctggcgcac
     4381 acttcgcggg gtgaaaacgg tttagccaca tagtcgtcag cgccaatttc cagcccaagc
     4441 aggcgatcga cctcttcact tcgggccgtc aggaacagta caggtaacgc cggatagagc
     4501 gccagtaact ggcggcacaa ttcaaagcca ctgatatcag gcaaaccaac gtcgagaatc
     4561 atgacgtcgg gaacctgctg gcgagcttta tccagcaccg gcaaacctcg ctcaaagacc
     4621 tcgacggcaa aaccttcctg ctgcaacatg tagaccagcg tgtcggctat cccttgctca
     4681 tcttccacta accagaccgt ttcccgttgc ataaatcgcc tctgttattg ccgccacggc
     4741 atgacaggaa ccgcgctaat cgcgtttttc ggcgaacctt ctacaacttt gtcggagtaa
     4801 gccagataag cgagcgcgtt gcgtttggcg tcataaaagc gcacgacctg taacgactta
     4861 aagaccaggg acgtgcgttt tttgaatact acctcgccct gagctttgcc gtttttaata
     4921 cgatccgaca gttcaatcgg cccgacttgc tgacaagaaa tggccgcatc ggaggtatct
     4981 tctgccaggc ccaatccccc tttaatacca ccggttttcg cccggctcac ataacaggtg
     5041 acatttttca catcgggatc gtcaaaggct tccacaacaa ttttgtgatc cgggccgatc
     5101 attttaaata cggtgtcgac cgaaccaatc tcttctgcat gagccaatgg ccccagcatt
     5161 atcaggctta aagacaggat caaatgcttg tatttcatat tgttaccatt tttatctatt
     5221 acacgagcaa ttagttcgtc acggtaaata acatattgat taagaacaaa atctcaattc
     5281 ttttatttcc gtcaagccct aaaacatact ctactaagaa aaaaacactg aatgctaaaa
     5341 cagcaaaaaa tgctattatc caattacctg atgtcaggtg ctcgttgttg aaaggatgag
     5401 gatattttat ggatcaggcc ggcattattc gcgacctttt aatctggctg gaaggtcatc
     5461 tggatcagcc cctgtcgctc gacaatgtag cggcgaaagc aggttattcc aagtggcact
     5521 tacagagaat gtttaaagat gtcactggcc atgctattgg cgcgtatatt cgtgctcgtc
     5581 gtttgtcgaa atcggcggtc gcactacgcc tgactgcgcg tccgattctg gacatcgcgc
     5641 tgcaataccg cttcgactct caacagacat ttacccgcgc attcaagaag cagtttgccc
     5701 agactcctgc actttaccgc cgttctcctg aatggagcgc ctttggtatt cgcccgccgc
     5761 tacgtctggg tgaattcaca atgccagagc acaaatttgt caccctggaa gatacgccgc
     5821 tgattggtgt tacccagagc tactcctgtt cgctggagca aatctctgat ttccgccatg
     5881 aaatgcgtta tcagttctgg cacgattttc tcggcaacgc gccgaccatt ccgccggtgc
     5941 tctacggcct gaatgaaacg cgtccgagtc aggataaaga cgacgaacaa gaggtattct
     6001 ataccaccgc gttagcccag gatcaggcag atggctatgt actgacgggg catccggtga
     6061 tgctgcaggg cggcgaatat gtgatgttta cctatgaagg tctgggaacc ggcgtgcagg
     6121 agtttatcct gacggtatac ggaacgtgca tgccaatgct caatctgacg cgccgtaaag
     6181 gtcaggatat tgagcgatac tacccggcag aagacgccaa agcgggagat cgcccaatta
     6241 atctgcgctg tgaactgctg attcctatcc gccgttaacg ctgcagctca tctaatgcag
     6301 gggcgtccag atgcgagatg tcccctgcgg tttcaacgac ccagccagac gccagccaca
     6361 ggctttcctg ataatccacg cgcgaaatag aacagttacg cagacgtaag cggcgctctg
     6421 cccatgctgg taatccgaga atcgtactca ccagacatcc cagtgcaata ccatggctca
     6481 ccagcagcgg tcggcttccc tgcggtaagt cccggcaaga ttccagtgcc gcattgacgc
     6541 gatcgctgag ctcctgcatt gactcgcctt caggaatacg cccgtcaacg gtgccattga
     6601 ccagctgccg acgccagttc tcttcttctt cggtcagaga atcgatatgt cttttttcca
     6661 gcacacccat gtttaattca cgcaggcgag aatcaaagat gatgtcacag ccgcaggcct
     6721 gggcgatgat ttccgccgta cgccgggtgc gtcctaaatc gctactgata atatgcgtaa
     6781 tgccaagctc tttggcacgg gttgccacct gcatcgcctg ttgctcacct ttggcggtca
     6841 gcgggctgtc agactggccc tgaatacgtc gctcggcgtt ccactgcgtt tcaccgtggc
     6901 ggactaggta tacctgtaac atgctttttt tccgtatact gctgtaacgt taattttctg
     6961 agtaatgctg attatgcacc aagttgtctg tgcgaccacc aatcccgcta aaattcaggc
     7021 cattctgcag gcatttcacg agatcttcgg cgaaggatcc tgccatattg catccgttgc
     7081 cgtcgagagc ggtgtaccgg aacaaccctt tggcagtgag gaaacgcgcg ctggcgcacg
     7141 aaatcgggta gcaaatgccc gccgtttact tccagaggct gatttttggg tggcgattga
     7201 agctggcatt gatggcgaca gcactttcag ttgggtggtg attgaaaacg ccagccagcg
     7261 cggcgaggcg cgttcggcaa ctttgccgtt acctgcggtg attctggaaa aagttcgtga
     7321 aggcgaggcg cttggtcccg tgagtcgcgt tataccggta ttgatgagat tggtcgtaag
     7381 gaaggggcga tcggcgtgtt taccgccggg aaactcactc gcgccagcgt ctatcaccag
     7441 gcggtgatcc tggcgctgag tccgtttcac aatgccgtgt atcaggcatt gtaggacgga
     7501 taaggcgttc acgccgcatc cgacattcgg tgcacgatgc ctgatgcgac gctgccgcgt
     7561 cttatcaggc ctacaaaatc aatcgctttt cagcaacacc tcttccagcc actgacgcag
     7621 ctccacgggc gcggctttca ggctgttaga tccacgcgta atcgtcgcga tgcccgcgcc
     7681 gagttcattt tttaactcac gctgactcat ttcgccacgc aacagctctt cgacaatacg
     7741 cacgcgagtc cccaacgctt cgcgctcatc tggcgtcagc atcaggttta acaacggtaa
     7801 atggagatcg ttttggtagg cattcttaag caggtcgaca aaacgtaacc actcctggtg
     7861 acgctgttct gccatcgctg ctgaataggg tgattgttgg gccataatat gtcgccattg
     7921 ttagcggggg aagcaaaatg actcccccgg ttgtactcgc taaagagtac gatagcatat
     7981 cataaacgtg cggatcagta acgacgtccc cattccgtgg cgctcatcaa cgtcggttta
     8041 tcccccatga aatagcggta gtaagcatca taagccagca cgttcttcac ataaccgcgc
     8101 gtctcggaga acggaatact ctcgacaaat gccacagcgt cgatacgccc ggcgctgttg
     8161 ccaagccagg ttcgcacccg tccaggtccg gcgttataag ctgctgagga gaaaatacgg
     8221 ttattgccaa actgctgata aacatattgc aggtaactgg tgccaatgtt gatgttcgtt
     8281 tccggatcca gcaattgccc agggctgcta taaccaggaa tagagaacat cttcaccgta
     8341 tgggtcgctg taccaggcat aatctgcatc aggccgctgg cccctaccgg tgatttcact
     8401 ttcggattcc aggcgctctc ctgacgggca atcgccatcg catagctttg cgggatctct
     8461 ttcccactgg tgtaacgttt gaaaagatcg ttgtaagcca gcgggaatcg ctcttccaga
     8521 tgatcccaaa gcttcccggc gatcgttgcc tgaacgctaa gatcccacca ttggttattg
     8581 aaagcatacc gagccagttg agcctgctct gtttttgact tgctcttcac cagattggcc
     8641 cactcgctac gcgcggtatt atcgagattc cagtacatca actcgcgcac gcgcgccatc
     8701 tccggcccct gagtcagggt gctgtcaaca ttctgcggcg ctttatcaat cttcagctca
     8761 tactcttcac cgatgcgttg tgctgcaacc atcgggtaga aaccacgctg ttgtatgagt
     8821 tgatgcaaaa tctctttcgc ttcagcttca cgtccgcgtt ccagcaataa atccgcctgc
     8881 cagtaacgcc attcatcttt ctcttttgct tccatcggca gacgcgccag ccaggtattc
     8941 aggccgcgac gatcgccggt gccaagcgcc attcgcacac ggcgttcaat caacgacgtg
     9001 gattgcgagc gcataatggc gtcatcgcgc catttcgcct gctcatcggt gacatcattg
     9061 cccatcaaac gccaggcgac gatatcgcgc agctcctgaa tctgatcttc attaagctgc
     9121 tgcgcctggg caagcgacgg gatcatcagc cgtgcatttt cggcatcctg ccgcgccaca
     9181 ctggcaaacg ccaccgccgc catttgacgg gtaaaatcgg tcgcgccggt tgtacgcgcg
     9241 aaggtcagta ccgtattagg gttgttcgcc agtgaaatga ttgccgaggc gatagtctgg
     9301 taatcggcag gcatctgccc tgccagcact gttaccaggc ctgtgttacc cgctttcatc
     9361 gccagacgga tacgctctaa atacgccagc ggatcttgtt tacctgacgc acgccagacg
     9421 ctaaataact tgtcacaggc gttaggctgg ctcttgccgg ttagccacag ctctttcgcc
     9481 ccttgccagg cttcttcact ctgcccggtg ttccatttcg catagtagta attacattgc
     9541 gcttcggtag ttccgggctt ttccgggcta aaggctaaca agccacgcca gtcttcacgc
     9601 cgcgccagtt cattgacgaa acgagattgc agcgtgcgag cgggaggaag cgtggggtta
     9661 gcgcgaacaa agttagtgac cgtcaccgcc ggttgattca tcaggtcatc ggtgatctgg
     9721 cggtattcca ggtagggata aagcggataa tccttcagtc caggcatcat ttgttcgacc
     9781 acatccatct gtcgattatc ccaggcctgc ttgatttgcg cgtaacggct acgctgctca
     9841 tccagtgagt cggctcgcgc cacgctgctg accgtcagca gacagacacc ggcagccaac
     9901 agccgccagg taacttgttt ggctttttcc acaagcgcat cctctaagtg taaatacatc
     9961 aatgcagcgt catgccgcgt aatagattta tgctaaccag tcattgccgt ttacgccacg
    10021 ttacggacac ttttttactt ttactgcgaa ggtgatcgac caactgggat taggcgggga
    10081 aacaggctac acttcgcctt tgaaaactca tcatcacgat aaacatagag gcgaagtcca
    10141 acgtggctca attcgtttat accatgcatc gtgtcggcaa agttgttccg ccgaaacgtc
    10201 atattttgaa aaacatctct ctgagtttct tccctggggc aaaaattggt gtcctgggtc
    10261 tgaacggcgc gggtaagtcc accctgctgc gcattatggc gggcattgat aaagacatcg
    10321 aaggtgaagc gcgtccgcag ccagacatca agattggtta tctgccgcag gaaccgcagc
    10381 tgaacccgga acacaccgtg cgtgagtcca ttgaagaagc ggtttcagaa gtggttaacg
    10441 ccctgaaacg cctggatgaa gtgtatgcgc tgtacgccga tccggatgcc gattttgaca
    10501 agctggccgc tgaacaaggc cgtctggaag agatcattca ggctcacgac ggtcataacc
    10561 tgaacgtaca gctggagcgt gcggcggatg cgctgcgtct gccagattgg gacgcgaaaa
    10621 tcgctaacct ctccggtggt gagcgtcgtc gcgtggcgtt gtgccgcctg ctgctggaaa
    10681 aaccagacat gctgctgctc gacgaaccga ccaaccacct ggatgccgaa tccgtggcgt
    10741 ggctggaacg cttcctgcac gacttcgaag gcaccgtggt ggcgattact cacgaccgtt
    10801 acttcctcga taacgttgca ggctggatcc tcgaactgga ccgcggtgaa ggaattccgt
    10861 gggaaggtaa ctactcctcc tggctggagc agaaagatca gcgtctggcg caggaagctt
    10921 cacaagaagc ggcgcgtcgt aagtcgatcg agaaagagct ggagtgggta cgtcagggaa
    10981 ctaaaggccg ccagtcgaaa ggtaaagcac gtctggcacg ctttgaagag ctgaacagca
    11041 ccgaatacca gaaacgtaac gaaaccaacg aactgtttat tccacctgga ccgcgtctgg
    11101 gcgataaagt gctggaagtc agcaacctgc gtaaatccta cggcgatcgc ctgctgattg
    11161 atgacctgag cttctcgatc ccgaaagggg cgatcgtcgg gatcatcggt ccgaacggcg
    11221 cgggtaaatc gaccctgttc cgtatgatct ccggtcagga acagccggac agcggcacca
    11281 tcactttggg tgaaacggtg aaactggcat cggttgatca gttccgtgac tcaatggata
    11341 acagcaaaac cgtttgggaa gaagtttccg gcgggctgga tattatgaag atcggcaaca
    11401 ccgagatgcc aagccgcgcc tacgttggcc gctttaactt taaaggggtt gatcagggta
    11461 aacgtgttgg cgaactttcc ggtggtgagc gcggtcgtct gcatctggcg aagctgctgc
    11521 aggttggcgg caacatgctg ctgctcgacg aaccaaccaa cgacctggat atcgaaaccc
    11581 tgcgcgcgct ggaaaacgcc ctgctggagt tcccgggctg tgcgatggtt atctcgcacg
    11641 accgttggtt cctcgaccgt atcgccacgc acatcctgga ctaccaggat gaaggtaaag
    11701 ttgagttctt cgaaggtaac tttactgagt acgaagagta caaaaaacgc acgctgggcg
    11761 cagacgcgct ggagccgaag cgtatcaagt acaagcgtat tgcgaagtaa tgcgtaaaat
    11821 gccggatgcg gcgcgaacgc cttatccggc ctacaaaacc agcaatttca atacgttgta
    11881 aaactgtagg cctgataaga cgcggtaagc gtcgcatcag gcattgagca ccgaatgccg
    11941 gatgcggcgt gaacaccttt tccggtctac aacagcctta gcatcctcaa tttcgtggcg
    12001 ggtcacccgt gcaggttttt tcaaaccttg tttaatctcg acagcctctt ccagagaggt
    12061 tttcaactca tcaaaaaaac tcattttata cctcgccttt gagcagtttc gtcacggtta
    12121 tccttgctcc cccatcatct cccgcaccag ctccacgcag cgcaggaaac ggctgtcgta
    12181 atcgtcctct tcaacccgca cgaattcgat attgttctct tccagcatct ccaccagcaa
    12241 gttctggaac tctttgcgat ccaccgaact gccgaggctg cgtaaaccat ccgccaccca
    12301 cggcgtgttg ttctccagca ggatcaccag atcgaaacgg tattcatcaa tcagcgcctg
    12361 cacgaacgga tgctcacgcc cttcgtactt tttgcagaac gcctgggtag tgacaaaatc
    12421 ggtgtcaata aacgccactt tattggcgta tttcactgca aaatcaatgt attgtgcgtg
    12481 acccaacgcg attttatcgt agtcggaata ctgcaatgcg atttcatcac cgccgaggtg
    12541 tgaaaagaca taatcgcggc catattccca cgcactggtg gtgttgaaga tattggcaag
    12601 tttgtttacc agggtggatt taccgctcga ctcgccgcca aggatcgcca cggtacgcac
    12661 aaaaaacggc ttcacttcgg taggaatata ttcccagtag cggaacgggt tttcgcggat
    12721 ctgcgcaccg ctgatactca taaaggtacg tttcggatcg accagcaccg tctcgatccc
    12781 cagatgttcc atatactgtg gcgcatcggc ttcttccgag gtgtagatca ggtccggctg
    12841 gatccccttt tcagccataa actttttgat gccgttgctc cacacatccc agccatgcgg
    12901 atacggctcc atgccctctt cgttgaaagc atgaatacgg atatttttct gatatttaaa
    12961 ggtttgcaat agccagcgca gacgatccgg caccgtgggc tgctgcgaca tggcgctgtc
    13021 ttcaaacaac gcgcggtcgc gggtatcgtc aaaacccata atgatatgca gctcgtcaac
    13081 ctggctacag gcgcgctgga taaggtagat atgtccggta tgcagcgggt agaacttacc
    13141 gaatacgaca ccaatggttt tcttctgccg gggaaattca agccccaaaa aacggtgcaa
    13201 tgcctccagc ttttgcgcgc tggggctttt aattttggca ttcagtaact ggcttaaata
    13261 ccctttggtc ataccgctgg catcagctac ctgctgtagc gtgcagccct gttgcttgat
    13321 ggcagttttc aggtaatcaa atgacgacat atctccctcc gtatctctca ttataagtcg
    13381 tcgaacacgc taagcgcgtc ggagagtttt ttaacgccaa aaatctgcat cccttccggc
    13441 gcttttttcg gtacgttagc cgccggaaca atcgcccggc gaaaaccgtg tttcgccgct
    13501 tcagaaatcc gctcctggcc actgggcacc gggcggatct cccctgccag cccgacttca
    13561 ccaaacacca ccagatcctg tggcagcggt ctgtcacgca ggctggaaac catcgccagc
    13621 agtaacgcta agtcggcgct ggtttcggtt accttcacgc cgccgaccac gttcacaaac
    13681 acatcctgat cggccatttg cagaccaccg tgacggtgca agacagccag caggattgcc
    13741 agacggtttt gttccagccc cactgccacg cggcgcggat tcgccattat cgagtgatcg
    13801 accagcgcct gaatctccac cagcaacgga cgcgttcctt cccacaccac catcacggag
    13861 ctgccggagg tcacttcatc tccgcgactt aagaaaattg ccgaagggtt gctgacttca
    13921 cgcagcccct gttcggccat cgcgaagacg cccagctcat tcaccgcgcc gaagcggttt
    13981 ttatggctgc gtaaggtgcg aaaacgggag tcggcatcgc catccagaag caccgaacag
    14041 tcgatacagt gttccagcac tttcgggcca gccagcgaac catctttggt tacgtgtcca
    14101 accatgacaa tcgccacacc gcgcgttttg gcgaaacgcg tcagataagc cgccgtttca
    14161 cgcacctgcg ccacgctacc aggcgatgat tgtacatccg ccatatgcat cacctggatc
    14221 gagtcaatta ccatcagctt cggttgctct tcttcggcaa tcaggcagat ctgctcgatg
    14281 ctggtttccg acaacatatt gagattgtca gtcggcaggc caaggcgatg agcgcgcatt
    14341 gccacctgtt gcagcgactc ttcgccggtg acatacagcg ttttcatctg ctgggccagt
    14401 ttgcacagcg tttgcagcaa cagcgtggat ttccccgcac cagggttacc gccaatcaga
    14461 atggcacttc ctggcaccac gccgcctcct agtacgcggt cgaactcttt aaatccggtg
    14521 gaaaaacgcg gcagctcttc aaggctgata tcggagagtt tctggacttt tgccaccccg
    14581 gcgctaccgg catagccgct gagacgctcg ttacgcgcca ccgttggcga cgcagcaaga
    14641 cgcacctcgg tgatggtgtt ccaggcatga caggcactgc actgcccctg ccagcgcgga
    14701 taatcggccc cgcattcatt acaaacaaag gcgcgttttg gagcttttgc cacggtgtac
    14761 ctcgttaatg ctgtgccgcc cgcaggatga cgggcaggca attacttctg attcaggctg
    14821 ccagagagga tgcagaatac ccccatcagg tcagcgtgac ggatggtgac ttccgccttt
    14881 tcattcactt ttggcttggc atggtaggca atccccagcc ctgctgcttt gatcatcggc
    14941 aggtcattag ctccatcgcc aatcgccacg gtctgcgcca gcgggatttc atactcctgc
    15001 gcgaggcgag tcagagtttt cgctttgtac tgcgcgtcta cgatgtcgcc gatcacattg
    15061 ccggtaaatt taccgtccat gatctccagt tcattggcta ccacggctgt caggcgcagc
    15121 ttgtcgcgca ggtattcagc aaagaaagta aagccgccgg aggcaatcgc cactttccag
    15181 cccagcgttt ccagcttgag caccagctgc gttaagcctg gcatcagcgg cagattttca
    15241 cgcacctgtt gcagaatatt ggcgtcagcg cctttcagcg tcgccacgcg gctgcgcagg
    15301 ctggcggtaa aatcgagttc gccgcgcatc gcccgttcgg ttacctccgc caccatctcg
    15361 cccgttccgg ccagcttggc aatttcatca atacattcaa tctggatggc ggtggaatcc
    15421 atgtccatca ccagcaaacc cggcgtgcgc aggtgcggga ttttccccag cggggcgaca
    15481 tccagctgcg cttcgtgggc caggcgtgta gcccgtgcgg tgagtgaacc tgccagacga
    15541 atcacctgat aatcttccac gcaccaggcg gcaacaatca ccatcgccgc acccagtttg
    15601 ctctggtatt gggtcagacg ctgtttatcc agcccacgac catacagcag ccagccgcta
    15661 cgacctgcgt ggtaatccag tggcatcact tcatcaccac ttaatgaaag aggcagaccc
    15721 ggccataaag agacatcttc aggcaggtcg caccaggtaa tgttaggcat taaggctcct
    15781 gtaaaatcgt tcgaagcagg gaaaataacg catgaggcta ccttgtatcc attgcttctg
    15841 gcaacattaa gtctcaaatt ttcaaagggt ggaagatggc tcgcacaaaa ctgaaattcc
    15901 ggctacatcg ggcagtgatt gtcctgttct gtcttgcctt gttagtggca ctgatgcagg
    15961 gggcgtcatg gtttagtcaa aaccaccagc gacagcgtaa tccacagctg gaagaactgg
    16021 cccgcaccct ggcgcgtcag gtgacgctga acgttgcacc gctgatgcgt accgactcac
    16081 cggatgaaaa acgcattcag gcgatcctcg atcagttaac agatgaaagc cgtatcctcg
    16141 acgcgggtgt gtatgacgaa caaggcgatc ttatcgcacg ttctggcgaa agcgtcgaag
    16201 tgcgcgatcg gctggcgctc gacggtaaaa aagcaggcgg ctattttaac cagcagattg
    16261 tcgagccaat tgcgggtaaa aacggaccgc tcggctatct gcgcctgaca ctcgacaccc
    16321 atacgctcgc caccgaagcc caacaggtgg ataacaccac taacatttta cgcctgatgt
    16381 tgctgctctc actggcaatc ggcgtagtgc tgacccgcac gctgctacag ggtaaacgca
    16441 cccgctggca gcaatcgccc ttcctgttaa ccgccagcaa accggtgccg gaagaggaag
    16501 aaagcgagaa aaaagagtga cccattacta caagaaagga aatcgttatg tccacattac
    16561 gcctgctcat ctctgactct tacgacccgt ggtttaacct ggcggtggaa gagtgtattt
    16621 ttcgccaaat gcccgccacg cagcgcgttc tgtttctctg gcgcaatgcc gacacggtag
    16681 taattggtcg cgcgcagaac ccgtggaaag agtgtaatac ccggcggatg gaagaagata
    16741 acgtccgcct ggcgcggcgc agtggcggtg gcggcgcggt gttccacgat ctcggcaata
    16801 cctgctttac ctttatggct ggcaagccgg agtacgataa aactatctcc acgtcgattg
    16861 tgctcaatgc gctgaacgcg ctcggcgtca gcgccgaagc gtccggacgt aacgatctgg
    16921 tggtgaaaac cgtcgaaggc gaccgcaaag tctcaggctc ggcctatcgc gaaaccaaag
    16981 atcgcggctt ccaccacggc accttgctac tcaatgccga cctcagccgc ctggcaaact
    17041 atctcaatcc ggataaaaag aaactggcgg cgaaaggcat tacttcagtg cgttcccgcg
    17101 tgaccaacct caccgagctg ctgccggggc tcaccatgag caggtttgcg aggccataac
    17161 cgaggccttt ttcgcccatt atggcgagca cgtggaagcg gaaatcatct ccccggacaa
    17221 aacgccagac ttgccaaact tcgccgaaac ctttgcccgt cagagtagct gagagtggaa
    17281 cttcggtcag gctccggcat tctcgcatct gctggatgaa cgctttagct ggggcggcgt
    17341 ggaactgcat ttcgacgttg aaaaaggcca tatcacccgc gcccaggtat ttaccgacag
    17401 cctcaacccc gcgccgctgg aagccctcgc cgggcgactg caaggctgcc tgtaccgcgc
    17461 ggatatgctg caacaagagt gcgaagcgct gttggttgac ttcccggacc aggaaaaaga
    17521 gctacgggag ttgtcgacgt ggatagcggg ggcggtaagg taactcgctg attaaaatat
    17581 ccaaggaagg aaatatgatc gatctggaaa accaggaacg ggagattatc aatttaatgt
    17641 tcagccagcg tatttcctgg ctagccgccg tgcgtatcag gcataaatta tcgcttgctg
    17701 aagtaagtaa aatgttaggt atctcgatta attctctaaa acagatcgaa aagacggaac
    17761 ggcttagctc aaacatcaaa agcaaaatgg cagaaattta tggttgtcca ccagagttgc
    17821 tgatatgccc ttcctggatg acggctgaac ataaatgaat agattcgtga atagatactt
    17881 gaatgttttt tattttctat ccatatgaaa aataatagta aatctataaa tcagttgcta
    17941 ttgatgaata gatactaaaa agccggagca gtcccccact ccggcttaac acgacaatcg
    18001 cctttcagcg aaacacaatt actctttatc gcccagcaga acggattcca gtgcgatttt
    18061 gatcatgtcg ttgaaggtgg tctgacgctc agcggcagtg gtctgctcgt gagtgcggat
    18121 gtggtcagac acggtgcaga tggtcagggc tttcgcgcca aactctgcag ccacgccgta
    18181 gataccagcc gcttccattt ccacgccgag gatgccgtat ttttccatca cgtcgaacat
    18241 ttcgccgtcc ggagagtaga acaggtcagc ggagaacagg ttacccacgc gagcatcaac
    18301 gcccagtgct ttagctgcat ctactgcgtt acgcaccatg tcaaagtcag cgatagcggc
    18361 aaagtcatgg tctttaaaac ggatgcggtt aactttggaa tcggtgcagg cccccatacc
    18421 gataacgacg tcgcgcagtt ttacgtgcgg cagaactgcg ccacaggaac caacgcggat
    18481 aattttcttc acgccgaaat cggtgatcag ttctttggtg tagatggagc aggacgggat
    18541 acccataccg tgacccatta cggaaatttt gcggcctttg taagtaccag tgaagcccag
    18601 cataccgcga acgttgttca cttcacgggc atcttcaagg aaagtttcag caatatactt
    18661 cgcacgcagc gggtcgcctg gcatcaaaac tacgtcagcg aaatcgccca tttctgcatt
    18721 aatgtgtggg gtagccattg ttttatcctt tttgtgacat aacaaaggcg gagtcaacgc
    18781 tccgcccaaa tccatcagaa catggctttg ccatattcca tatcagaagt accaaaatat
    18841 tttgccagag tctggccgat atccgcgaag gtttcacggt gacccagtga gcccggtttt
    18901 actttcgggc catataccag taccggaatg tgttcacgcg tgtggtcagt accggtccag
    18961 gtcggatcgc aaccgtggtc agcggtgagg atcaggatgt cgtcatcgcg cagcagagac
    19021 atcagctccg gcagacggcg gtcgaacagt tccagacccg cagcataacc ggcgacgtcg
    19081 cggcggtggc cccaggaaga gtcgaagtca acgaagttgg tgaagacgat ggtgttatca
    19141 cccgcttctt tcatctcttt gatggtggcg tcaaacagcg cgtccaagcc agtcgctttc
    19201 acttttttgg tgataccgca gttggcgtag atgtctgcaa ttttaccgac agaaaccacc
    19261 tggccgtgtt tttcatcaac cagtttctgc agcacggtcg gtgctggcgg ctcaacagcc
    19321 aagtcgtgac ggttaccggt acgctggaag ttaccagctt tgtcaccgat aaacggacga
    19381 gcgataacac gaccgatatt gtagccgccg ttggtcagct cttcacgggc gatttcgcac
    19441 agttcgtaga gtttatccag accgaaagtt tcttcatggc aggcaatctg gaacacggag
    19501 tcagcggagg tatagaaaat cggcttgccg gttttcatgt gctcttcgcc cagttgatcc
    19561 agaatgaccg taccggaaga gtggcagtta ccgaggtaac ccggcagatt agcgcgttcg
    19621 accagtttat ccagcagctc ttgcgggaag ctgttttcgt gatcggagaa atatccccac
    19681 tcaaacagaa ccgggacacc ggcgatttcc cagtgaccag acggggtatc tttaccggat
    19741 gacatttcgt gcgcccatgc gtacgcgccg ataacttcag cgttgccgtc cattcccgcc
    19801 ggaatgaaac cggtagaacc ttcgtgtgct ttcgccagcc ccagacgggt cagatttggc
    19861 agattgagcg gccctttacg accattatca gcttcgcctt tggcacaagc ttctgcgata
    19921 tgacccaggg tgtcagcccc gacgtcacca aagcgttctg catcttctgt agcgccgatg
    19981 ccgaatgagt ccagcaccat aataaatgca cgtttcatat gttctccgta ctcagtgctt
    20041 caaatttaaa tgatcagatc agtataccgt tattcgctga tacggcgata gacagttggt
    20101 gtgctttccg gtgcgttatc ggcaagttta attgccgctt tcaccgcttt cgccgcttcc
    20161 tgccagctgt tttcgtcttt cgcgaggata accgccagcg gacgctgacc gtctacctgg
    20221 tcgcccagac gcgccatatc agtaaagccg acgctgtaat cgatggtgtc agatgcctga
    20281 cgacgaccgc cgcccattgc aactactgcc atccccagcg cgcgggtatc catttcactg
    20341 acaaaacctt cggtatcagc atagactgct ttcgtcagca tcgctgtcgg cagatacttc
    20401 gcgtagttct caacgaagtc ggtcgggcct ttttgtgccg ctaccatacg accaaagact
    20461 tctgccgctt taccgttgtc cagcaccgcc tgcaatttcg cgcgcgcttc ggcgtcatct
    20521 ttcgccagtt tgccggagat caacatctcc acgcacagcg ccatcgtgac atcaaacaga
    20581 cgcgggttac gatactcacc cgtcaggaac tgcaccgctt cacgaacttc aaccgcgtta
    20641 cctgcactgg aggccagtac ctgattcatg tcggtgagca gcgcggtggt gcgcacgcca
    20701 gcgccgttag ccacgccaac aatcgcttcg gcaagggctt cagagagttc gtaggtcggc
    20761 ataaacgcgc cgctacccac tttcacgtcc atcaccagcg cgtccagacc ttccgccagt
    20821 ttcttcgcca ggatcgaggc agtgatcagc gggatggagt ccacggttgc ggtaatatca
    20881 cgggtcgcgt agaaacgttt atcagccgga gccagtgaac tggtctgacc gataatcgcc
    20941 acgccgacgt ctttaataat ttcgcggaaa cggttgtcat ccgggaaaat gtcgaagcca
    21001 gggatggatt ccagtttgtc gagcgtaccg ccagtatgac cgaggccgcg accggagatc
    21061 atcggaatat agccgccgca ggctgcgacc atcggcccca acatcagcga agtgacatcg
    21121 ccgacgccgc cggtcgagtg tttatcaaca atcgggccat tcagatgcag gcttttccag
    21181 tcgagaacgg ttcctgaatc tcgcatcgcc atggtcagcg agacacgctc aggcattgtc
    21241 atatcgtgga agaaaatggt catcgcgagg gcggcaatct gcccttcgga aatagtgttg
    21301 tcgcgaatac cgttgataaa gaaacgaatt tcttcatcgc tcagcgcatg accatcacgt
    21361 tttttacgaa taatttcttg tgcgagaaac aaggtaacct cccgaggaaa agagtatgag
    21421 gcggcttttg ccggatgcgt ctacgcctta tccggcctac gggtagatac gtctcgtagg
    21481 cctgataagc gcagcgcatc aggcgtaaag catcttactt agtagctgct ggcgctctta
    21541 ccgtcgccgt ggcccagcgc tttcagcagg cttgccagca ggctggaagc accaaagcgg
    21601 tagtgacgcg catcagccca gtcagcaccg aacagttcat ctgcgatggc gagatatttc
    21661 tgcgcatctt ccgcagtacg cacgccgccc gccggtttga aaccaacggt tttttctacg
    21721 cccatatcac ggatcacttc catcatgatg cgagcgcttt ccggcgtcgc gttcacagcc
    21781 actttaccgg tagaggtttt gatgaagtcc gcacccgctt tgatggagat ttcagacgct
    21841 ttacggatca gcgcttcgtc tttcagttcg ccggtttcga tgatcacttt cagcagtaca
    21901 ttcgctgccg cgcaagcctc tttacaggct ttcaccaggt caaaaccaac ctgctcgtca
    21961 cccgccatca gcgcgcggta cgggaacacc acgtcaactt catcagcacc gtaggcgatt
    22021 gccgcacggg tttctgccag cgcgatttcg atgtcgtcgt taccgtgtgg gaagttggtt
    22081 accgtagcga tacggatttc cggggtgccc tgctctttca gagttttgcg agcaatcggg
    22141 ataaaacgag gatagataca gatagcggcg gtattgccga ccggagtttt ggcctgatga
    22201 cacagagcaa ttactttctc gtcggtgtcg tcgtcattca gggtggtcag gtccatcaat
    22261 ttcagtgcac gcaggctgct tgctttcaga tcagtcattt cattctcctg gcttgtcgcc
    22321 aataaaattc accttgcgag tttgttagta ttctaacatc tactccgcaa cacacttcga
    22381 tacacatcac aattaaggaa atctacttac aagtttgcat cactgtaatg cgatctggtt
    22441 caaataattc actttcaaat gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga
    22501 acatcacgaa acttcttaca atggcgcatc accttcggca taaaaggaaa agaagatgcc
    22561 cacttctcat gaaaatgcac tgcaacaacg ttgccagcaa attgtcacca gcccagtgct
    22621 tagcccggag cagaagcgcc attttctggc actggaagca gaaaacaatc tgccttaccc
    22681 acagcttcct gccgaagccc gccgcgcgct ggatgaaggt gtaatctgcg atatgtttga
    22741 aggtcatgcg ccgtacaaac cgcgctatgt cttacccgat tacgctcgtt ttctggcgaa
    22801 cggttccgaa tggctggagc tggaaggcgc gaaagatctt gatgacgcac tctctctgct
    22861 gaccattctt taccaccacg taccgtcggt cacatcgatg ccggtctacc tggggcaact
    22921 ggatgcgttg ttgcaaccgt atgttagaat tctaacacaa aacgagatcg atgttcgaat
    22981 aaaacgtttc tggcgttacc tcgacagaac cctgccagat gcctttatgc acgccaatat
    23041 cggcccgtct gattcgccca ttacccgcgc gatcttacgt gcagacgcgc aggtttcacc
    23101 gaacctgacc tttatctacg atcctgaaat cacccctgat gacctgctgc tggaagtggc
    23161 gaagaacatc tgtgaatgta gcaaaccgca catcgccaac ggtccggtgc atgataaaat
    23221 tttcacaaaa gggggctacg ggattgtgag ctgttacaac tcactgccgc tggcgggtgg
    23281 tggcagcacg ctggtacgcc ttaacctgaa agccattgcc gagcgcagcg aatcgctgga
    23341 tgacttcttt acgcgcactc taccgcacta ctgccagcag cagatcgcca tcatcgatgc
    23401 gcggtgtgaa ttcctctatc aacaatcaca cttctttgag aatagcttcc tggtgaaaga
    23461 agggctgatt aaccctgaac gttttgtgcc aatgtttggc atgtatgggc tggcggaagc
    23521 ggttaacttg ctgtgtgaga aagaagggat tgccgcgcgc tacggtaaag aagccgccgc
    23581 aaatgaagtg ggttatcgca tcagcgcgca actggcggag tttgtcgcca atacccccgt
    23641 gaaatatggc tggcaaaaac gcgccatgtt acacgcacag tcggggatca gttccgatat
    23701 cggcaccacg ccgggcgcgc gtttgccgta tggcgatgag ccagatccga tcacccatct
    23761 gcaaactgtc gccccgcatc atgcttatta ttattccggc atcagcgaca ttctgacgct
    23821 cgacgaaacc atcaaacgta acccgcaggc actggtacag ctttgcctcg gtgcctttaa
    23881 agccggaatg cgtgaattta ccgccaatgt cagcggtaac gatctggttc gcgttaccgg
    23941 ttatatggtg cgtttgtcgg atttagaaaa atatcgcgcc gaaggttcac gcaccaacac
    24001 cacctggctg ggcgaagaag ccgcacgcaa cactcgtatt ctggaacgcc agccgcgcgt
    24061 gataagccat gaacagcaga tgcgctttag tcagtaagat tatccccttc tcctgcgttg
    24121 acgggccagg cagtcgtctg gctctgtttt tgcagggctg caatctgcgc tgcaaaaact
    24181 gtcacaatcc gtggacgatg ggacgttgca atgactgtgg ggagtgcgtg ccacagtgtc
    24241 cgcatcaggc gttgcagatt gttgacggca aagtggtgtg gaacgctgtg gtctgcgagc
    24301 aatgtgatac ctgcctgaag atgtgtccgc aacatgccac accaatggcg caatccatga
    24361 gcgtggacga agtgcttagc catgtgcgca aagcggtgct gtttattgaa gggataacgg
    24421 taagcggcgg cgaagccacg acccaactgc cgtttgtagt ggcgctgttt actgctatca
    24481 aaaacgatcc gcaactgcgc catctcacct gtctggtgga cagtaacggt atgttgagcg
    24541 aaaccggctg ggaaaaatta ctcccggtgt gtgacggcgc aatgctcgat ctcaaagcat
    24601 ggggtagcga atgtcatcaa caattaaccg gacgcgataa tcaacagatt aagcgcagca
    24661 tctatttgct ggcagagcgc ggcaagctgg cggaactgcg tttgctgatg atcccaggcc
    24721 aggtggatta tttgcaacac atcgaagaac tggcggcgtt tatcaaggga cttggtgatg
    24781 ttccggttcg cctgaacgcg tttcatgccc acggcgtgta tggcgaggcg caaagctggg
    24841 cgagcgccac gccggaagac gttgagccgt tggctgatgc attaaaggtg cgcggggtga
    24901 gccggttgat atttccggcg ctctatttgt gacatgccgg atacggccta cggttcggca
    24961 cagacttgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcattgtg cgccaactgc
    25021 cggatgcggc gtgaacgcct tatccggcct acggttcggc acaaacctgt aggcatgata
    25081 agacgcgtta agcgtcgcat caggcattat gcgccgactg ccggatgctg cctacgaagg
    25141 gcaaactacc cacgaacgtt aaacaccgca tacgtgttat taagcaacac ttcggcaatc
    25201 tcatccgcgg gttccgggcg taactcgcaa agcacggcaa acacacgggc agcctgttcc
    25261 gggcggttag gctgcccctg aaaaccgttg agcggcatat ccggcgcatc ggtttccagc
    25321 aacaacgatg ccagcggtaa ttttgcgatg acatcgcggg ttttactggc gcgtggatag
    25381 gtgatagtac cgcctacgcc gattttgtag cccagctgta caaaccgttc agcctgttgc
    25441 tggctgccag aaaatccgtg gacaacacca gcacgcggca aatcgtggcg tttaagatgc
    25501 atcgccagtt tgtcgtgcgt gcgccgtgaa tgcaggatca ccggcagatc gtagcgtttc
    25561 gccagtttca gttgttcgtc gagtaaccac tgctgcctct caaattgcgg atcgtcgccg
    25621 aagagatcca gaccgatctc ccccaccgcc accaccttcg ccggacgcct ttccagcgcc
    25681 tgctgtagct gctcaagaga cacatcgcta tgtttttcca acataccagg atgcaagccc
    25741 aatacggcat acagcggttg ataattttcc gctaacgcct gcacacgggc aaaattttcc
    25801 gcctctgtcg ccggaacaat gatcttgcct acgcccgctt gtgccgcgcg ttgcaggctg
    25861 gcctcttcat cgccaataaa aggcgggaaa tcaaaatggc agtgagtgtc gataaaacgg
    25921 caaatcaagc cagatcctca ttgttaaatg tcgggtcgtt cgcctgcgga gcgtcgctca
    25981 cttcggcaac cagcgtatcg ttggcgactg gcgcaggcgg aatgacaatc gattccggcg
    26041 tcaccactgg caccagatgg cgggtaagcg gcgctttttc agttaatagc ttgccaaccg
    26101 tggcgaggaa ataacgaccg cataaacgcc caagtttata gtcttcacgc agcgccggaa
    26161 tccgactgcc aagcgcgata ctatgtaatg gcttcggcgg ataaatttcg gatatccgca
    26221 gcttgcccgg tggtttctca ataaattgct gaatgtcacg atagctggtt tcatgatgct
    26281 gcaccagatt gaccagcggc tgcaggctac tgtcacccag ccagcgttcc atgcgtttga
    26341 accactgcgg cgtgtagtac atttgtgacg gcacagtgcg gatgacgacc aacgttttag
    26401 cgccctgcct tgccgcctct ttaaccggaa tcgcatcact gatcccgcca tccaggtagt
    26461 taatgccttc cagcgacact ccgctacgat aaaagccagg tatcgcactg gaggcgcgaa
    26521 tcacatccag ccagttttgt ttggttggta aaaagtaatt cggcgcgtag tcatcctgac
    26581 gacaggcgca catataaaac gatttgccgc tgtcaaacaa ccgcgcggcg gtatccattt
    26641 gcaacggcat ctggcttgcg gtggcctcca ccagccagtc gagatcgata agatttcctc
    26701 cacggacaaa gcgcaatgga tcgaaaaatt cgcgttttgt ggtatagcgc atgatgactt
    26761 tgcgcgcgta accgggctga ttgcagataa acgccgagag gttctgcgcc ccggcagatg
    26821 tgccgagata aagatcgaaa ggattaaact gcgcgcgcat aaactcatcc agcacgccag
    26881 ccgtgaaaat tccacgctgt ccgccgcctt cgcacaccag agctattcgt ccaggctgga
    26941 acggccttag cgacaacggc gcaatattac caagcgttac aggtattcgc tgccccactt
    27001 ctgccttcct gtttttgttg tgaaagaaag acaaagtaac gcaatttatt attcgctaaa
    27061 accgcaaaag ccagtccgct ggactggctt tggataaaat tttcttaatg gtctgaaatc
    27121 tagggtcgtt ttcggcccat gaacaaactc actaggaaca gaataatccc gacgacaaag
    27181 acaattttag ctgcgcctgc agcggtaccg gccagaccac caaacccaag tgcggcggcg
    27241 attaacgcga taaccagaaa tatgatgccc caacgaaaca taagcgtctc ctttaccata
    27301 gttaatgtca ccgcttgata tgagcgtgaa aatcactcaa tgcgatattt ttagtgtggt
    27361 gcacatcgcg cctcccgaca aagttcggga ggacgaatta cttagttttc agatcatttt
    27421 taacgctttt cacaccatct accgctttgg cgatactttc agcacggtca ctttgtgcct
    27481 gagaatctac ggtaccggag agctgaacca cgccatcggt ggtttcaact ttcactttac
    27541 gtgaagggac gatatcgtcc gccagcagtt tggctttgat ttcactggtg gtggcggtgt
    27601 cacccgcgta gcccttcacc gagccttctt tagcgtcgcg aacgtgcagt ttgtcgctga
    27661 cagaggtcac gccttcaacg cctttcgcca ctttcactgc ctcttcggcc tgggcctgac
    27721 tttcaacgaa accgctcagg gtcacgactt tttgatcggt tttgacagag atatcggtgc
    27781 tcttgatgtt gtcatgatcc accagcgccg ccttcacttt cgcggtgatg gcgctgtcat
    27841 ccatgaaatt accgacttta ttcatagagc tatcgacttt ttgccctgcg ctttcattgg
    27901 tagtctgcgc gttgttttcc gcgtaggcag agccggtcgc gacggcagag gtcaacatta
    27961 cagccagcag agttttcgaa atcttcagtc ttgtcatagt catcgattta ttcctgtatg
    28021 tttgctcgta atttgagcct agcaacacga ggttgcattg ctgaataggg agagacttca
    28081 ccctctacag aagtcaatgg tcgccatcac aaaagcgatg ggtgatgaat aacgaccatt
    28141 acagcctctg aatcagttat taatatcggt agaatgacaa tcgacggctt tagatactaa
    28201 tatctacgca ttgaacggta tttaacgcca tcagaaatgt catcactttg ttaaatatag
    28261 atcacaattt tgaaaccgct cgggatatca gcgaaaacat aagcaaaagt gaatgtttta
    28321 agaacattcc gtaagcggct aataaggaag ggaaattgac agggcgcagc ggctaccgcg
    28381 ccctggaggc aagaattaat gctcgcgggt ctggtggaac tgaacgtccg gataacgttc
    28441 ctgtgccagg cgcaggttga ccatgctggt agcgatgtaa gcgaggttat cgccgccatc
    28501 aagcgccagt tggctttcgt tcttacgctt gaactcttcg aatttcttcg cgtctgcaca
    28561 ttctacccag cgggcagtgg caacgttgac tgactcatac actgcttcaa cgttgtattc
    28621 gctcttcagg cgcgctacca ccacatcaaa ctgcagcaca ccaaccgcac caacgatcag
    28681 atcgttgttg gagattggac ggaacacctg caccgcgccc tcttcggaaa gctgtaccag
    28741 ccctttgagc agctgttttt gcttcagcgg atctttcagg cggatacgac ggaacagttc
    28801 tggtgcgaag ttcggaatac cggtgaactt catcatctca ccctgggtaa aggtgtcgcc
    28861 gatctgaatg gtgccgtggt tgtgcaggcc gaggatatcg cccggatacg cttcttcaac
    28921 gtgcgaacgg tcacccgcca taaaggtcag cgcgtcggag atcaccacat ctttcgcagt
    28981 gcgcacctgg cgcagtttca tgcctttttc atatttaccg gacaccacac gcataaacgc
    29041 cacgcggtcg cggtgtttcg gatccatgtt ggcctgaatt ttaaatacga agccggtaaa
    29101 tttatcttcg ctcgcttcta cggtacgggt atcggtctga cgcggcatcg gcgcaggcgc
    29161 ccactccacc aggccattca gcatatgatc aacgccgaag ttacccagcg cagtaccgaa
    29221 gaataccgga gtgatttcgc ccgcaaggaa cagctctttg tcgaactcgt tagacgcgcc
    29281 tttaaccagt tccagttcgt cacgcagctg ctgtgccaga tcttcaccaa ccgcagcatc
    29341 gagatccggg ttattcagcc ctttaacaat gcggacttcc tgaatggtgt gccctttacc
    29401 gctctgatag agataggttt catctttata aaggtggtaa acgcctttaa acagcttgcc
    29461 gcagccaatt ggccaagtga tcggtgcgca gccaattttc agctcgttct caacttcatc
    29521 gagcagttcc atcgggtcgc ggatatcacg gtcaagtttg ttcataaagg tgaggatcgg
    29581 cgtgtcgcgc agacgggtaa cttccatcag cttacgggta cgatcttcaa caccttttgc
    29641 ggcgtcgata accatcaggc agcagtccac cgccgtcagg gtacgatagg tatcttccga
    29701 gaagtcttcg tgccccgggg tgtcgagcag gttaaccagg caatcgtgat acggaaactg
    29761 catcacagac gtagtaatgg agatcccacg ctgcttttcc atctccatcc agtccgactt
    29821 agcgtgctgg ttggaaccac ggccttttac tgtaccggcg gtctgaatgg cctgtccgaa
    29881 cagcagcacc ttctcggtga tggtagtttt accggcgtcc gggtgagaaa taatggcaaa
    29941 agtgcggcgc ttcgccacct ctttcaaata aggagacaac gtcataattc tttcttcttg
    30001 agtaagcggc atcgcgccgc gcttgttgaa aacgaaaaat tgcggctatt ttacccatca
    30061 acggggggga ggcaatcagt gtttacacag gagctgctcc agttcgtgca acgaagaaac
    30121 ggtccaggtg ggcgcgatgc cttctggttg ctcgcgattg tgcgcattca gccagcaggt
    30181 cgcaagcccg gcgttgatgc caccgagaat atcggactcg gcagtgtcgc caaccatcag
    30241 cacgcgtgaa cggtcaggat tgcccgcctg ttccagcgca taatcgaaaa ttttcttatt
    30301 cggtttggca acgccaactt cttcggaaat caccagcaaa tcgaagtaat cacgcaggcc
    30361 cgtccgttcc agacgcactt gctgcaaggc gctaaaaccg ttggtgatga tgccgatttt
    30421 agcgttgcta cgaatggcgt taagcagaga aaccgcgccc ggcaacggcg tacagatttc
    30481 cgccatcgca ttaataaagg catcgttaag caagcctgct tcaactttca gccgttcggc
    30541 ccagctctcg aaacgcccgt gctgaagctg taatgaagtg atcgcgccgt tttgataatc
    30601 cacccacagt ggcttgttaa cggcctgata gtcctgaaaa tcttcagcgg taaaggtgac
    30661 gctgtaatca agaaacatcc gctgcaggcc ggtgaatgag tcaaaggtaa acagcgtttc
    30721 atcggcatca aagaaaatcc agtcccactt cattattcca ccttgtctta catactgatt
    30781 ggcaacgcca tgatgatggc gtcttcgcga ccgtccgtgg tggggtagta attgcggcga
    30841 atcgtcgcct cgttaaagcc taaactttcg tacagggcaa tggcggcagc atttgaggca
    30901 cggacttcca gccatagtgt cgccacgccg cgtttttcca gttcgtcgat cagatgttcc
    30961 agcagcgccc tacccagccc ctgacgctga tagtcgggat cgaccgcaat attgaacaat
    31021 gtagcttcat ccagcaccac ttgcgtaatc gcaaacgccg ccattttgcc gttttgcgtt
    31081 aactgaaagt tgagataacg ctcgccctgg ttgctggcaa aggttttttc actccacgga
    31141 aaggcgtggg cgcgttgttc aatgtggtaa gccgctggta aatcagtcgt ttcgagggaa
    31201 gaaatcgtgt tcatatgtgc aaatttgttg ccataacgcg gcgcgtgccg ttgggtttgc
    31261 ccgtaattcg gtgagcgccg gtgatgccac ctgagcgcct tccaatgata gcggttcgtc
    31321 agtacccaac cgccagctgt tgcagcgact gccttgcggc agcatcgcga ttttttctgg
    31381 cgtcagttgc agcacttgat cggggctgac ggttaatgcg cgcagaacat cgctcactaa
    31441 aggatcagtc agggcgggaa gatcgtttgc caccatcacc agacggacgt gtgccgggct
    31501 cgcaatggca atctccccct gcaacgcgcc agggcgacgc agcgaccact gggtaatgcc
    31561 cagttgctgt aactgccagt ctcgtcggga tgtcatagcg aatcgctcct gttgtcaggg
    31621 gcgcaaatat agcaaattcg tcgataccgc gccaacatat ggctataatc gccgccagta
    31681 tcaattgagg agcattccat gtctgcattt accccggcaa gtgaagtctt gctgcgtcac
    31741 agtgatgatt tcgaacaaag ccgtattctg tttgccggag acttacagga tgacctgccc
    31801 gcgcgtttag ataccgcggc cagccgtgct catacccagc aattccacca ttggcaggtg
    31861 ttaagctgcc agatggggga taacgcccgt tttagtctgg tcgccacggc gaatgatgtc
    31921 gcagattgcg atacgctgat ttactactgg ccgaagaaca aaccggaagc ccagttccag
    31981 ttgatgaatt tactttctct gctgccagtg gggacagata tttttgtcgt tggcgagaac
    32041 cgcagcggcg tgcgcagcgc tgagcagatg ctggcagatt atgcgccgtt gaataaagtc
    32101 gacagcgctc gtcgctgtgg cctctatttt ggtcgtctgg aaaaacagcc agtatttgat
    32161 gcggataaat tctggggcga atacagcgtc gatggcctga cggtcaaaac gctgcctggc
    32221 gtgtttagcc gcgacggtct ggatgtcggt agccagttgc tgctctcgac gttaactccg
    32281 cacacgaaag gtaaagtgct ggatgtcggc tgtggcgcgg gtgtactttc agttgccttt
    32341 gcgcgccatt cgccgaaaat tcgtctcacc ttgtgcgatg tctctgcccc ggcggtagaa
    32401 gccagccgcg caacacttgc agccaacggt gttgaaggtg aagtctttgc cagcaacgtc
    32461 ttttccgagg tgaaaggttg ttttgatatg atcatctcca acccgccgtt ccacgatggg
    32521 atgcaaacca gcctggatgc ggcgcaaacg ctgattcgtg gcgcggtgcg tcatcttaat
    32581 agcggcggcg agctgcgaat tgtagcgaac gccttcctgc cttacccgga cgtgctggat
    32641 gagacatttg gcttccatga agttatcgcg caaaccgggc gcttcaaggt atatcgcgcc
    32701 attatgaccc gccaggcgaa gaaaggttga gtatctgata tctgtcgccg cataccgtgt
    32761 cggatgcggc gtgaacgcct tatccgacct acaaaacctg cacgttagcc cttcgtaggc
    32821 cagataagac gcgccagcgt cgcatctggc atctgccccc gcatgcctac caaacctgca
    32881 cgctagtttc ctgatggaca tttttccagc aattacacat caggcgataa ttaactattg
    32941 acgaaaagct gaaaaccact agaatgcgcc tccgtggtag caattctttt taagaattga
    33001 tggtatgcga aggtggcgga attggtagac gcgctagctt caggtgttag tgtccttacg
    33061 gacgtggggg ttcaagtccc ccccctcgca ccaaaaatca cgttgatatt gctcgcactg
    33121 ggcgaaggtg gcggaattgg tagacgcgct agcttcaggt gttagtgttc ttacggacgt
    33181 gggggttcaa gtcccccccc tcgcaccaaa cgaggcgata tcaaaaaaag taagatgact
    33241 gtgcgaaggt ggcggaattg gtagacgcgc tagcttcagg tgttagtgtc cttacggacg
    33301 tgggggttca agtccccccc ctcgcaccaa ttatctttac ttcctttctt gtttcttccc
    33361 tgttttgtca tccctgattc aaattcatta tcatcagcat gatgccagca ataagcgcca
    33421 ggcacgatgg tagcaaaatg taatgccgca actgtttgtg atacagcatc accggcgtca
    33481 atactaatcc catcagaatg acaatccgcc actgatccgg cgataaatcc gcatacatca
    33541 gccctgcaat cagcaatccc ggcagcaaca ctccccagcc actgcccagc gtacgttgca
    33601 acatggtttc atctctttca ttgataatga aaaccaatat catatgataa tttttatcat
    33661 ttgcaagcca gagaaatccc ttgctatcga gtaaacctat cactaggatt agcaatcatt
    33721 atcatttaga ttactatcac gattatggcc tatcgttccg caccgctcta tgaagatgtc
    33781 atctggcgaa cgcatctcca gccgcaggat gctgggcttg cacaagccgt acgggcgatg
    33841 atcgccaaac atcgtgaaca tttgctggag tttatccgcc tggatgaacc cgctccgctt
    33901 aacgccatga cgctggcgca atggtcatca ccgaatgcgc taagttctct actggcggtc
    33961 tattccgatc atatctatcg caaccaacca acgatgatcc gcgagaacaa accgctgatc
    34021 tcactatggg cacaatggta tatcggcctg atggtgccac cattaatgct ggcgctactg
    34081 acgcaggaaa aagcattaga tgtgtcgccg gaacatttcc atgctgagtt tcacgaaacc
    34141 ggacgcgtcg cctgtttctg ggtcgatgtg tgtgaagata aaaacgcaac accacattcg
    34201 ccgcagcagc gaatggaaac gttaatcagc caggcgctgg ttccggttgt gcaagcacta
    34261 gaagcgaccg gagagatcaa cggcaaactt atctggagta ataccggtta tttgatcaac
    34321 tggtatctca ctgagatgaa gcaactgctc ggcgaggcta ctgttgaatc gctgcgccat
    34381 gccctctttt ttgagaaaac gctcacgaac ggtgaagata atccactctg gcgtaccgtg
    34441 gtactgcgcg acggcctgct ggtgcgccgc acttgctgcc agcgttatcg cttaccggat
    34501 gtgcagcaat gtggctattg tacgctgaaa tgaaagcact gccggggaag taaacccggc
    34561 atcatgcgga ttaataggga tgcaacacat tacttgcttc gcaatgccgc atacataaca
    34621 gaatatcttc ggcctccaac aaccctgcat cagaactgac gcccagtttc gacatcacat
    34681 taaatttatg cgctcggatc gttttgatat tacgtttaag ctgctcggca atttgtgtca
    34741 ttgagtagcc acgcgacata aagcgcaata tttctctctc cgtcgggctt aacgtccggc
    34801 tttggttaat gtaccactga ttgttcagtc ggtcggtcgc ctgacgtaca ccatttaatg
    34861 acaaaaagag ttcctgatga aaaatctcca gcgttgacgc tttacttaat acaccgtcca
    34921 gcggtgatgg cgacaatgaa ccaatcagcc gagcttcaat atcatcatcc acaataacta
    34981 aacgccgggt acgcggaaac ttaatcgcca gctcagtcag gcaagataat ccctctctgc
    35041 gctcacttct catggcagaa aaagaaaaaa tgaccgccgc aaacgagatc cgcgacatgg
    35101 catcctgaaa tgactcctga ctggtatagg tatggagctt ataagggttg cccgcaaact
    35161 ttctgaataa actctcaata ccgatactac tcatgacgca tttttctatg agtgcgacat
    35221 ttctcttctt aattcggctg tgttccattc tgcggcatct ccataaacag aaggcaagtt
    35281 aagttctctt gcaccctgcg cgctatttat ccatgcatac atttcagcgt tgctgtgaat
    35341 tgccagtcga cgcatcgcac tgtttttttg ggcgctgata gttttattac ttttcttaag
    35401 caatgaagct atctggttga tgccccatcc tttacctaat agccgcagta ccttgcgctc
    35461 tgaaagagtg agaatgacgg agcgatagct aaaatcatga gtttcagtca ttgccggaga
    35521 ggtcagcatg gcgctgatac gctctgcgtg cgtattgccg gaacgtacgg tcttgaccag
    35581 attctcaatg ggttcaacat cagacaataa cgtcgccgta gggcacatga gcagttctac
    35641 agcctgggaa taccaggagc gtgacaccat gaaaacccag tgaatttccc gatattgtga
    35701 gattaaagaa taataatgtt cacaaacaga acgtggatct tcactttcac cggctaaatc
    35761 agcaatgact aa
//
LOCUS       DAZXUL010000035        34903 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZXUL010000035 DAZXUL010000000
VERSION     DAZXUL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34903
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.035"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(587..730)
                     /gene="ypaB"
                     /locus_tag="ACH3L1_001668"
     CDS             complement(587..730)
                     /gene="ypaB"
                     /locus_tag="ACH3L1_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFT4076440.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            858..3143
                     /gene="nrdA"
                     /locus_tag="ACH3L1_001669"
     CDS             858..3143
                     /gene="nrdA"
                     /locus_tag="ACH3L1_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFT4076441.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3377..4507
                     /gene="nrdB"
                     /locus_tag="ACH3L1_001670"
     CDS             3377..4507
                     /gene="nrdB"
                     /locus_tag="ACH3L1_001670"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFT4076442.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4507..4761
                     /gene="yfaE"
                     /locus_tag="ACH3L1_001671"
     CDS             4507..4761
                     /gene="yfaE"
                     /locus_tag="ACH3L1_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFT4076443.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="ACH3L1_001672"
     CDS             complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="ACH3L1_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFT4076444.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5545..5649)
                     /locus_tag="ACH3L1_001673"
                     /pseudo
     CDS             complement(5545..5649)
                     /locus_tag="ACH3L1_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5644..5886
                     /locus_tag="ACH3L1_001674"
                     /pseudo
     CDS             5644..5886
                     /locus_tag="ACH3L1_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="ACH3L1_001675"
     CDS             complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="ACH3L1_001675"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFT4076445.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="ACH3L1_001676"
     CDS             complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="ACH3L1_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFT4076446.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8640..10268
                     /gene="glpA"
                     /locus_tag="ACH3L1_001677"
     CDS             8640..10268
                     /gene="glpA"
                     /locus_tag="ACH3L1_001677"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFT4076447.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10258..11517
                     /gene="glpB"
                     /locus_tag="ACH3L1_001678"
     CDS             10258..11517
                     /gene="glpB"
                     /locus_tag="ACH3L1_001678"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFT4076448.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11514..12704
                     /gene="glpC"
                     /locus_tag="ACH3L1_001679"
     CDS             11514..12704
                     /gene="glpC"
                     /locus_tag="ACH3L1_001679"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFT4076449.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12897..13847
                     /locus_tag="ACH3L1_001680"
     CDS             12897..13847
                     /locus_tag="ACH3L1_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFT4076450.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13888..14349)
                     /locus_tag="ACH3L1_001681"
                     /pseudo
     CDS             complement(13888..14349)
                     /locus_tag="ACH3L1_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(14413..15615)
                     /locus_tag="ACH3L1_001682"
     CDS             complement(14413..15615)
                     /locus_tag="ACH3L1_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFT4076451.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15702..16244)
                     /locus_tag="ACH3L1_001683"
     CDS             complement(15702..16244)
                     /locus_tag="ACH3L1_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFT4076452.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16523..16948
                     /gene="nudI"
                     /locus_tag="ACH3L1_001684"
     CDS             16523..16948
                     /gene="nudI"
                     /locus_tag="ACH3L1_001684"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFT4076453.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16987..17589)
                     /gene="ais"
                     /locus_tag="ACH3L1_001685"
     CDS             complement(16987..17589)
                     /gene="ais"
                     /locus_tag="ACH3L1_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFT4076454.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17897..19036
                     /gene="arnB"
                     /locus_tag="ACH3L1_001686"
     CDS             17897..19036
                     /gene="arnB"
                     /locus_tag="ACH3L1_001686"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFT4076455.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19040..20008
                     /gene="arnC"
                     /locus_tag="ACH3L1_001687"
     CDS             19040..20008
                     /gene="arnC"
                     /locus_tag="ACH3L1_001687"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFT4076456.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20008..21989
                     /gene="arnA"
                     /locus_tag="ACH3L1_001688"
                     /pseudo
     CDS             20008..21989
                     /gene="arnA"
                     /locus_tag="ACH3L1_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21986..22876
                     /gene="arnD"
                     /locus_tag="ACH3L1_001689"
     CDS             21986..22876
                     /gene="arnD"
                     /locus_tag="ACH3L1_001689"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFT4076457.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22876..24528
                     /gene="arnT"
                     /locus_tag="ACH3L1_001690"
     CDS             22876..24528
                     /gene="arnT"
                     /locus_tag="ACH3L1_001690"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFT4076458.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24525..24860
                     /gene="arnE"
                     /locus_tag="ACH3L1_001691"
     CDS             24525..24860
                     /gene="arnE"
                     /locus_tag="ACH3L1_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFT4076459.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24860..25246
                     /gene="arnF"
                     /locus_tag="ACH3L1_001692"
     CDS             24860..25246
                     /gene="arnF"
                     /locus_tag="ACH3L1_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFT4076460.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="ACH3L1_001693"
     CDS             complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="ACH3L1_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFT4076461.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25617..26972)
                     /gene="menE"
                     /locus_tag="ACH3L1_001694"
     CDS             complement(25617..26972)
                     /gene="menE"
                     /locus_tag="ACH3L1_001694"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFT4076462.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26969..27931)
                     /gene="menC"
                     /locus_tag="ACH3L1_001695"
     CDS             complement(26969..27931)
                     /gene="menC"
                     /locus_tag="ACH3L1_001695"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFT4076463.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27931..28788)
                     /gene="menB"
                     /locus_tag="ACH3L1_001696"
     CDS             complement(27931..28788)
                     /gene="menB"
                     /locus_tag="ACH3L1_001696"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFT4076464.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28803..29561)
                     /gene="menH"
                     /locus_tag="ACH3L1_001697"
     CDS             complement(28803..29561)
                     /gene="menH"
                     /locus_tag="ACH3L1_001697"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFT4076465.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29558..31228)
                     /gene="menD"
                     /locus_tag="ACH3L1_001698"
     CDS             complement(29558..31228)
                     /gene="menD"
                     /locus_tag="ACH3L1_001698"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFT4076466.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31317..32612)
                     /gene="menF"
                     /locus_tag="ACH3L1_001699"
     CDS             complement(31317..32612)
                     /gene="menF"
                     /locus_tag="ACH3L1_001699"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFT4076467.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="ACH3L1_001700"
     CDS             complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="ACH3L1_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFT4076468.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33051..33512)
                     /locus_tag="ACH3L1_001701"
     CDS             complement(33051..33512)
                     /locus_tag="ACH3L1_001701"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFT4076469.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33577..34494
                     /gene="rbn"
                     /locus_tag="ACH3L1_001702"
     CDS             33577..34494
                     /gene="rbn"
                     /locus_tag="ACH3L1_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFT4076470.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34685..34873
                     /locus_tag="ACH3L1_001703"
                     /pseudo
     CDS             34685..34873
                     /locus_tag="ACH3L1_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8537 a         9340 c         8648 g         8378 t
ORIGIN      
        1 cgtaatgcct gaaaggcttt gcctgctggc gtgacaactc gctttgatat catatccctg
       61 gcataaatca atggccgccg cgacaccgtt agcagagaaa agagatgcaa tcattgacgg
      121 gagtagagat aaatactcct tgcgtagaaa gataatccgc atattaatct gaccatcatt
      181 ttatataaga cggcatattt aaacgcatta tacaatatgg gttatctttc ttttgtaaat
      241 tgtccaaccc gatgcaatgg ctattttgtt attatttgat gaataatatc agtgcgtcat
      301 aattcaagtt aataacctta agggatacca gttatattta aactaaatta aagtaatgaa
      361 taattttctt ataatataag gtaaattaac aaaatggctt cgcatttaac aataaccgaa
      421 tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat tgaccacaac
      481 tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa
      541 agattaataa accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg
      601 aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac
      661 ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca
      721 agagggtcat tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt
      781 tctaagcagc ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa
      841 aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag
      901 agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata
      961 acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga
     1021 cctctgacat ccacgaaact attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc
     1081 cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg
     1141 gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat
     1201 acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta
     1261 tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat
     1321 atctagtaca gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc
     1381 ttgttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc
     1441 gtttttatga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg
     1501 tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg
     1561 attccatcaa cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg
     1621 gcatcaacgc cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc
     1681 ataccggctg tatcccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg
     1741 gcggtgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa
     1801 gcctgctggt gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact
     1861 acggggtaca aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc
     1921 tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt
     1981 ttgaacgcct gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag
     2041 ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc
     2101 agaacgttga ccactgtaat acccatagtc cgtttgatcc gaccatcgcg ccagttcgcc
     2161 agtctaacct gtgcctagaa attgccctgc cgaccaaacc gctgaacgac gtcaacgacg
     2221 agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc
     2281 tggatgaact ggaagagctg gcgattctgg cggttcgtgc acttgacgcg ctactggatt
     2341 atcaggatta cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt acgctgggta
     2401 ttggtgtgat taacttcgct tactatctgg cgaagcacgg taaacgctac tccgacggca
     2461 gcgccaacaa cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt
     2521 ctaatgagct ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga
     2581 aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc
     2641 tgcattacga ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca
     2701 cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta
     2761 ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg
     2821 tggtgccgga ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta
     2881 acgatggcta cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg
     2941 ctaacaccaa ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc
     3001 tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc
     3061 gtgacggcgc tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg
     3121 aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg
     3181 gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg
     3241 ccggatgcag cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac
     3301 gcgccagcgt cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc
     3361 ccaacaggac acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa
     3421 agaaccgatg ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga
     3481 catcttcgaa aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga
     3541 cgtctcccgc gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat
     3601 cagcaacctg aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc
     3661 gctattgccg cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc
     3721 agaaacgatt cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc
     3781 tgttgtgttt gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc
     3841 cagctattac gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac
     3901 ccacaccgtt aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta
     3961 tctctgcctg atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg
     4021 ttcctttgcc tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat
     4081 tgcccgcgat gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag
     4141 cggcgcggat gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga
     4201 cctgtttgtc caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg
     4261 ttcgatgatt ggtctgaata aagatattct ctgccagtac gttgaataca tcaccaatat
     4321 ccgcatgcag gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg
     4381 gatcaacacc tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag
     4441 ttcttatctg gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca
     4501 gctctgatgg cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa
     4561 cacccttccc ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgccgc
     4621 gaaggttact gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc
     4681 gaaccgttag cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc
     4741 gatattgaaa tcgagttgtg atcgggtact agcgccagat acgacgctta cgcgtattat
     4801 ctggcctaca acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgcttta
     4861 gggatcggct ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga
     4921 cgcaagcggc gacgactttt ctccagatca agaaaaccag cttctgcctt accttctgtt
     4981 ttcacataga tatggcgaac atagcaacag ccatgctgac gattaacgct atgcattttc
     5041 ttaaacgcca gtgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat
     5101 actgcatgcc gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc
     5161 agcaacgcgc gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg
     5221 atgacaccgg cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg
     5281 ccgaacggat aacgtactga atgaaacaga tgatgcgtca tgcgctttac atacagcttt
     5341 ttgccgttgc gctcgacgca ttgcaccccg ctcataccat tacggcgata gttaggctct
     5401 tcaacccagt cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact
     5461 gccataccct atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagg
     5521 aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt gccgctacag gtgtgacacc
     5581 gcttttgccc ttcatttgca gcgcatagat tccaccagcc actaccacta atagtgcgcc
     5641 cagcagataa aatgtgaatt taatcagtcg gatacagcga tgaattttat tcgacaggga
     5701 ttaggcatcg ccttacaacc agagttaacg ctgaaaagca ttgcaggtga attgtgttcc
     5761 gttcctcacg aaccaacctt ctatcgacag atttcgttgc tgactaaaga aaagccggta
     5821 gaaggcagtc cactgttttt actacaaatg tgcatggaac aattagtggc gattggaaaa
     5881 atttgatatg agacaggatg gcgcatgaac gccatcctgc agtaacatta ctctttatta
     5941 agaaatttta ctgccttatc agggaaatca gtaaacagcc catttacacc cgctttgtta
     6001 tacagagcat catataactg attcacatca ggagtgtatt caggcagttt atctgaccgc
     6061 acggtataag gatgcactac cagtttattc tgctgggcat cttgcaccat gccagtgagt
     6121 ttgatattac ccggctgcga tgtctcctca atcaacatat ggtaatccgg accaatacca
     6181 tctgcatatt ccgccacctg tttcatggct cccggcttaa acatccagtc gtagttgtaa
     6241 ttaacccagc ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc
     6301 agctgtacca gattgagctc catgcccatt ttgggttcca gctcattctt aatacgcttc
     6361 agctcatcag catcaaaaca ttgcaaataa actttatcgt ctttaccggt gtaaccatat
     6421 ttcttcagca cttccagcgt ttttgccgca atatccttcc cttcctgatg atggaaccac
     6481 ggcgctttga tttctggata gataccgata tttttcccgg tagagtgatt taacccctga
     6541 acaaattcaa tctcttcttc aaaggtgtgc acccggaagt cggacttacc cattgggaaa
     6601 cgccccggat aagtctgtac ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc
     6661 agcgacttaa tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc
     6721 cgatccggga aacgatcggc aacatcagta acacgatcga gataatggtc atgcagaaca
     6781 accagatggt cgtctttggt catcaccaaa tcctgttcca gataatccgc tccctgcgca
     6841 tacgccatcg cttttgctgg cagcgtatgc tccggcaaat atccactggc accgcgatgg
     6901 gcgatgacta ttttttcgtt gctgtccgcc gccattgcac tgcttcccat gactatagtg
     6961 ctcatcatga tcgccatgct aaggtttttc agcttcaatt tcatgccatt agcctccgtt
     7021 gcgtttttgc agtaattgtt catggcgacg tttttcgcca atcatcacaa caatcaacaa
     7081 gataaccgcc agaatgctgc cgccaatcat taccataaag ccgccatccc agccgaagaa
     7141 gtccacggtg tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag
     7201 cccggtaaag cccgctgccg tacctgccgc ttttttcggt gccagttcca gcgcatgcag
     7261 accgatcagc atcacaggac cgtagatcag gaagccgata acaatcatac aaatcatatc
     7321 gacggttggg ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt
     7381 cataaagaag acgccggttg ccccacggtt accacggaag actttgtccg acatccagcc
     7441 gcacagcagg gtgcccggaa tacccgcata ctcatagaag aagtaggccc aggaggattt
     7501 atccagcgcg aaatgcttaa cctctttcag ataagtcggt gaccagtcga ggatgccgta
     7561 acgcagcaga taaacgaaca cgttggcgat ggcgatgtac cacagcagtt tgttcggcag
     7621 cacgtactgc atgaagattt gcttcgcggt cagctcctgt tccgcttttt cgttatagtc
     7681 gtccggataa tcatttttgt actcttcgat cggcggcaag ccacaggatt gcggggtatc
     7741 gcgcatcatc gcaaaggcga ataatgccac cagaatggcg cagaaagcag gcatatagag
     7801 cgccgcatgc cagtcattga accaggccat ccccagcagg aacagcagcg gcggaatacc
     7861 accaccgacg ttgtgcgcac agttccacac tgacacaatg ccgccacgtt ctttctgcga
     7921 ccaccagtgc accatagtac gaccacacgg cggccacccc atcccctgga accaaccgca
     7981 gaggaacaac agtacaaaca tcaccgcaat gctcgacgtc gcccatggca caaagcccat
     8041 aaacaacatc actgccgccg ccagaatcaa acctgcgggc aggaaaacgc gcggattcga
     8101 gcgatccgat accgaaccca tgatgaattt cgaaaatcca taagcaatcg agatccccga
     8161 aagggcaaaa cctaaatcac cgcgtgagaa tccctgctca accagataag gcatagcaag
     8221 cgcaaagttc ttacgaacca aatagtaagc cgcatagcca aagaatatcc ccaggaaaat
     8281 ttgccagcgc aatcgacgat aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg
     8341 tggcgctggt ttaaaaatac tcaacattga tagcctccgt ggcccgtggt cttatttatg
     8401 attaacagcc tgattcagtg agagaacctg ccgtttcttg agttgccgcg atgttaagaa
     8461 aacattcata aattaaatgt gaattgccgc acacattatt aaataagatt tacaaaatgt
     8521 tcaaaatgac gcatgaaatc acgttttact ttcgaatcat gagcgaatat gcgcgaaatc
     8581 aaacaattca tgtttttact atggctaaat ggtaaaaaac gaacttcaga gggataacaa
     8641 tgaaaactcg cgactcgcaa tcaagtgacg tgattatcat tggcggcggc gcaacgggag
     8701 ccgggattgc ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg
     8761 acatcgcaac cggtgccacc gggcgtaacc acggcctgct gcacagcggt gcgcgctatg
     8821 cagtaaccga tgcggaatcg gcccgcgaat gcattagtga aaaccagatc ctgaaacgca
     8881 ttgcacgtca ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg gaagatgacc
     8941 tctcattcca ggccactttt attcgcgcct gcgaagaagc agggatcagc gcagaagcta
     9001 tagacccgca gcaagcgcgc attatcgaac ctgccgttaa cccggcactg attggcgcgg
     9061 tgaaagttcc ggatggcacc gttgatccat ttcgtctgac cgcagcaaac atgctggatg
     9121 ccaaagaaca cggtgccgtt atccttaccg ctcatgaagt cacggggctg attcgtgaag
     9181 gcgcgacggt gtgcggtgtt cgtgtacgta accatctcac cggcgaaact caggcccttc
     9241 atgcacctgt cgtggttaat gccgctggga tctgggggca acacattgcc gaatatgccg
     9301 atctgcgcat tcgcatgttc ccggcgaaag gatcgctgct gatcatggat caccgcatta
     9361 accagcatgt gatcaaccgc tgccgtaaac cttccgacgc cgatattctg gtgcctggcg
     9421 ataccatttc gctgattggt accacctctt tacgtattga ttacaacgag attgacgata
     9481 atcgagtgac ggcagaagag gttgatattc tgctgcgtga aggggaaaaa ctggcccccg
     9541 tgatggcgaa aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg gttgccagcg
     9601 atgacgaccc gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac catgctgaac
     9661 gcgatggtct ggacggattt atcaccatca ccggtggcaa actgatgacc tatcggctga
     9721 tggctgaatg ggctaccgac gcggtatgcc gcaaactggg caacacgcgc ccctgtacga
     9781 ctgccgatct ggcactgcct ggttcacaag aacccgctga agttaccttg cgtaaagtca
     9841 tctccctgcc tgccccgctg cgcggttctg cggtttatcg tcatggcgat cgcacgcctg
     9901 cctggctgag cgaaggccgt ctacaccgta gcctggtatg tgagtgcgaa gcggtaactg
     9961 cgggtgaagt gcagtacgcg gtagaaaatt taaacgttaa tagcctgctg gatttacgcc
    10021 gtcgtacccg tgtggggatg ggcacctgcc agggcgaact ctgcgcctgc cgcgctgccg
    10081 gactgctgca acggtttaac gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct
    10141 tccttaacga acgctggaaa ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa
    10201 gcgaatttac ccgctgggtt tatcagggac tgtgtggtct ggagaaggag cagaaagatg
    10261 cgctttgata ctgtcattat gggcggcggc ctcgccggat tactctgtgg cctgcaactg
    10321 caaaaacacg gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgtt gcatttctca
    10381 tccggttcgc tggatttgct gagccatctg ccagatggtc aacccgtggc agacattcac
    10441 agtggactgg aatctttgcg tcagcaggca cctgcacatc cttactctct tctcgggcca
    10501 caacgcgtgc tcgatctcgc ttgccaggcg caggcattaa tcgctgaaag cggtgcgcaa
    10561 ttgcagggca gcgtagaact ggctcaccag cggattacgc cgctcggcac tttgcgtgca
    10621 acctggttaa gttcgccaga agttcccgtc tggccgctac ccgcgaagaa gatatgtgta
    10681 gtggggataa gcgggctgat ggatttccag gcgcatcttg cggcggcttc gttgcgcgaa
    10741 ctggatcttt cggttgaaac cgcagaaata gagctgccgg aactggatgt cctgcgcaat
    10801 aacgccaccg aatttcgcgc ggtgaatatc gcccgtttcc ttgataatga agaaaactgg
    10861 ccgctgttac ttgatgcgct tattcctgtc gccaatacct gcgaaatgat cctgatgccc
    10921 gcctgcttcg gcctggccga tgacaaactg tggcgttggt tgaatgaaaa actaccttgt
    10981 tcactgatgc ttttgccaac gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag
    11041 ttacagcgcc agtttgtgcg ccaaggtgga gtgtggatgc cgggcgatga agtgaaaaaa
    11101 gtgacctgta aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc cgatattccg
    11161 ctacgtccac gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg actagtagcg
    11221 gaacgtaacg gcattcgcga gccgattctc ggccttgatg tgctacaaac cgccacgcgg
    11281 ggtgaatggt ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc
    11341 actgatgaga cgctacgccc gtcgcaggca gggcaaacca ttgagaacct gtttgccatc
    11401 ggttcggtgc tgggcggatt tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc
    11461 gtcagtgctt tacatgccgc tcaacagatt gcccaacgcg caggaggcca acaatgaacg
    11521 acaccagctt cgaaaactgc attaagtgca ccgtctgcac caccgcctgc ccggtgagcc
    11581 gggtgaatcc cggttatcca gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt
    11641 tgaaagatgg cgcactgtat gacgaggcgc tgaaatattg catcaactgc aaacgttgtg
    11701 aagtcgcctg cccgtccgat gtgaagattg gcgatattat ccagcgcgcg cgggcgaaat
    11761 atgacaccac gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta
    11821 gcgtttccac gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc
    11881 agctgcttga tgcggcgtta aaaatcgatc atcgtcgcac gctaccgaaa tactccttcg
    11941 gcacgttccg tcgctggtat cgcagcatag cggctcagca agcacaatat aaagaccagg
    12001 tcgctttctt tcacggctgc ttcgttaact acaaccatcc gcagttaggt aaagatttaa
    12061 ttaaagtgct caacgcaatg ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg
    12121 gcgtaccgct aatcgccaac ggctttaccg ataaagcacg caaacaggca attacgaatg
    12181 tagagtcgat ccgcgaagct gtgggagtaa aaggcattcc ggtgattgcc acctcctcaa
    12241 cctgtacatt tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac aacaaaggct
    12301 tgcgcgatca tatcgaactg gcaacccgct ggctgtggcg caagctggac gaaggcaaaa
    12361 cgttaccgct gaaaccgctg ccgctgaaag tggtttatca cactccgtgc catatggaaa
    12421 aaatgggctg gacgctctac accctggagc tgttgcgtaa catcccgggg cttgagttaa
    12481 cggtgctgga ttcccagtgc tgcggtattg cgggtactta cggtttcaaa aaagagaact
    12541 accccacctc acaagccatc ggcgcaccac tgttccgcca gatagaagaa agcggcgcag
    12601 atctggtggt caccgactgc gaaacctgta aatggcagat tgagatgtcc acaagtcttc
    12661 gctgcgaaca tccgattacg ctactggccc aggcgctggc ttaaactccc ttctgatgcc
    12721 cggtaagcat gtggttaccg ggcatttttg cgtacacgat tccgtgccca atgtatgcgt
    12781 tgcaacgcag tgaaaattcc tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca
    12841 aaggcctggg ataaccgtaa tgtcgcgtca tcataaatat caggtgacgg acaaccatga
    12901 ccgaatcaac aacctcctcc ccgcatgatg cggtatttaa aacctttata ttcacacccg
    12961 aaaccgcacg ggattttctc gaaatacatt taccagaacc actgcgcaag ctttgcaacc
    13021 tgcaaacctt acgcctggaa cccactagtt ttattgaaaa aagtttacgc gcttactact
    13081 cggatgtttt gtggtccgtg gaaaccagcg acggtgacgg ctatatctac tgcgtgattg
    13141 aacatcaaag ctctgcagaa aagaatatgg cttttcggcc aatgcgctat gccactgccg
    13201 ccatgcagag tcacctagat aaaggctatg acagagttcc gctggtggtg ccattgctgt
    13261 tttatcatgg cgaaacatcg ccctacccgt actcacttaa ctggctggat gagtttgacg
    13321 atccgcaact tgcccggcag ttgtacaccg aagcttttcc gttggtggat atcaccatcg
    13381 tacctgacga tgagatcatg caacatcggc gtatagctct gctggaactg attcaaaagc
    13441 atattcgcga ccacgattta atcggcatgg tcgacaggat caccacgctt ttggttagag
    13501 gcttcactaa tgacagccag ctacaaacac tgtttaatta tctgctgcaa tgcggcgata
    13561 cctcccgttt cacccgtttt attcaggaga ttgccgaacg ttcaccacta caaaaggaga
    13621 gattaatgac tattgctgaa cggctacggc aggaaggaca tcaaattggc tggcaagaag
    13681 gtaaattagt aggtttgcag caaggtaaat tagaaggttt gcaggaaggt atgcatgaac
    13741 aagccattaa aattgctttg cgcatgctgg aacagggcat tgatcgtgac caagtgctcg
    13801 cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta acacaggccc
    13861 acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg gcccattttt
    13921 gcctgattta aacatcgcca gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac
    13981 gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat caggagccac
    14041 agccagaaaa ccagccgctt tacccgcagc acggatccgc cgaatgctgg tttcaataat
    14101 tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag aaagatccgc
    14161 tggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca ggttatccag
    14221 tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt gcgccatgta
    14281 attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac cacgctcacc
    14341 gtagggaggg ttggacaaat tttgccggag ggggaatgtg ccggtgcagt aaaccggcac
    14401 agagtggcga aatcagacgg ataaagtcat ggactcaaca acctcaatcc agccatgctc
    14461 actagcgatg tcctggccgt ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca
    14521 cacttcctga cggatagcca ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa
    14581 agtgccgtct ggcgtggcta gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc
    14641 cagtgccagc ccggcaaaat ggttggcccg ccgttctgta atccagtgcg cagtttgcgc
    14701 cagggtttct tcctgtgaag gaatcacttc acacgccagc aatggagcac ctgcgcgaga
    14761 aagttgcaaa gccaataaac caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg
    14821 gttttgcaat tcgcgactga tctgcgcggg cagtccttcg gtgccttcga aaatcacgct
    14881 ctgtccggca acacgtttaa cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc
    14941 cggtccggtg agtttcagtt cgatgatcgg cattgaggag cgatagccca ttgttacgcc
    15001 cggcggcagt tgtagagtgt ccaggctttg tgccagatcg ctttccgaac gaccaaaggt
    15061 agttaaacgc agacaaaccg gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag
    15121 gatttcgtgc tcgaccatca ccttaaattc tgacggtacg ccgggagtaa agaacatcag
    15181 gcaacgatta agctgcacgg caaaaccaca ggcggtgcct accgggttat tgataaattc
    15241 agcactggca ggcagctccg cttgtttacg gttgctcggt gccattactc gtccacgttc
    15301 gtggaaatag cgttccatct ctttgagcca ggcttcatgc agcaccaggc cttcaccttt
    15361 tgctgtcgca gcggcgagtg cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac
    15421 gatcagcaca tcggcgtgct gactacgttc gcgaagaatg gtgactaagt catcaaggtt
    15481 atcccccacc gtattgcggc gagataatgg cagcccctga tgaaagaaaa aatcggccag
    15541 ccaggcagcg ttagtgtcaa cgatttgccc gtataacact tcatccccgg tggatagcat
    15601 ttccactttt aacatggtta tctcctgctt ttgttatcga atcactataa cgcaaaggga
    15661 gataaggcgt agtaagaaac tgaatgccgc gccggaaggg atcagaaact ggcgttaacc
    15721 ccaacataca ggccgtcagc cacagcgttg tcgcggttac cgtctttacc cgacagattc
    15781 aggtagcgat aacccgcctc aatactgact ggacgcataa tggtgtaacg cgcgccagca
    15841 ttcgcttcct cataactttg aataccgctg gagagcgaat ccggagagta gtaatactcg
    15901 ccaaacaaac ggaagctgtt gccaattttc cactgcaaac cacctcctac cgctgcggca
    15961 taaccttcat cgccgtaatt cgggttggtg tacacgcctt ttccgccaac ggtcgccatt
    16021 aacggcccga gaggcagatt caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg
    16081 ttatgtgtcc agttaccgct taaagccagg cccgtcgatt cagtcccaaa accgacacca
    16141 atattggtgt attctttacc cgcctggccg ctgatgctca ttgcattgac cgatgcagaa
    16201 accaacagca ttccggccag acctgctagc gcaatttttt tcattatccc ctttcccaca
    16261 agcaattaat caaaattccc aaagctgcgg gattgtactg cctggcacag aggattcaat
    16321 gagcgaaaaa agggatctag tcacttattg taactaaata cttcttaaat aattaaacgc
    16381 cgaaaaaata aacacacttt accgatacac acaaaaataa taataagtgc agttatcatt
    16441 gcaaaagaga ttactgtcac tttcacatat ttctgagtta ccgtattctc atggtttctt
    16501 tttatttata aggaataccc ctgtgcgaca acggactatt gtatgccctt tgattcaaaa
    16561 tgatggtgct tatttgctgt gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg
    16621 ggcgctttcg ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga
    16681 aattcgcgaa gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag
    16741 cgatgatatt cgcaccaaga catatgcaga tggtcgcaag gaagagattt atatgattta
    16801 cctgattttt gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga
    16861 ctacgcgtgg gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa
    16921 aacgttacgt ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt
    16981 gaaaatttag tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat
    17041 gactaaacca tccagataat caggtttaaa tatcgcatca cgcttatttt tagcaatata
    17101 tgtcaggcaa tgattatggg tgaaaataac gatattttta tcaggcgctt tgctttgtaa
    17161 gtccttaatt gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag
    17221 ctttttaccc gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata
    17281 aagatcgaaa tcagagatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt
    17341 acctttaacc gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg
    17401 ttcagcatgg cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt
    17461 attgtcgata cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat
    17521 tgcagcgagc gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa
    17581 agctaacacg taaactccac ctatagacaa gcgcaaccag acaattaccg tgaaattgag
    17641 cgacatttct ggcgataatt agcagttggt gtaatattaa aaatcctacg atgtcggcaa
    17701 aatgcctcaa atttttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat
    17761 atttacgtct aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct
    17821 ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat
    17881 ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga
    17941 ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa
    18001 tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag
    18061 ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga
    18121 agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct tgctgggtgc
    18181 aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga
    18241 atcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagc
    18301 agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc
    18361 tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat
    18421 tttttcattt catgccatta aaaatattac ctgtgctgaa ggtggcctga ttgtaactga
    18481 taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc
    18541 ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa
    18601 gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagtca aattagagca
    18661 cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc
    18721 ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat
    18781 tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga
    18841 aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga
    18901 gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcgtt
    18961 gccgctgttc ccggatatga ctaccgccga tgccgaccgc gtcatcacag cccttcagca
    19021 actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat
    19081 tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg
    19141 tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc
    19201 cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgat
    19261 taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg ttactggcga
    19321 cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt
    19381 ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag
    19441 ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca ccactggcaa
    19501 agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat
    19561 gttgcactgc catgaacgca gcacctttat cccgattctg gcgaatatct tcgcccgccg
    19621 tgcaattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca aatacagttt
    19681 tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg
    19741 tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct
    19801 ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt
    19861 tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac tcggtgaata
    19921 tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat
    19981 ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg
    20041 ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc
    20101 cataccgata atcccggtga aaaagccttt tatggttcgg tggctcatct ggcggcggaa
    20161 agagacattc cggtttatgc gccggataac gttaatcatc cgctgtgggt ggaacgcatt
    20221 gcccaactgt caccagaggt gattttctct ttttattatc gccatcttat ttgcgacgag
    20281 atttttcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt accaaaatat
    20341 cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca
    20401 ttgcaccgaa tggtaaacgt gccgatgctg gggccattgt ggcgcaactg cgcgttgcca
    20461 ttgcgccaga cgatatcgct attacgctgc atcataaatt gtgccatgcc gcgcgccagc
    20521 tactggaaca gacattaccc gccattaaac acggtaatat tctggaaatc gcccagcgcg
    20581 aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga cagtttcctt gaatggcata
    20641 aaccggcatc cgtactgcac aacatggtac gtgccgttgc cgatccgtgg ccgggtgcct
    20701 tcagctatgt tggcaatcag aaattcaccg tctggtcgtc gcgtgttcat cctcatgcca
    20761 gcaaagcaca gccggggagc gtgatttctg ttgcgccact gctgattgcc tgtggcgatg
    20821 gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat tactatgcag ggctcgcaat
    20881 tagcgcagat gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct gcctgcaccg
    20941 cccgacgccg tacccgggta ctcatcctcg gggtgaatgg ctttattggc aaccatctga
    21001 cagaacgcct gctgcgcgaa gatcattatg aagtttacgg tctggatatt ggcagcgatg
    21061 cgataagccg ttttctgaat catccacatt ttcactttgt cgaaggcgat atcagtattc
    21121 attccgaatg gattgagtat cacgtcaaaa aatgtgatgt cgtcttgccg ctggtggcga
    21181 tagccacgcc gattgaatat acccgcaacc cgctgcgcgt atttgaactc gattttgaag
    21241 agaatctgcg cattatccgc tactgcgtta agtaccgtaa gcgaatcatc ttcccgtcaa
    21301 cttcagaagt ttatgggatg tgtagcgata aatacttcga tgaggaccat tctaatttaa
    21361 tcgtcggccc ggtgaataaa ccacgctgga tttattcggt gtcaaaacaa ttacttgatc
    21421 gggtgatctg ggcctatggc gaaaaagagg gtttacagtt caccctcttc ctcccgttta
    21481 actggatggg accacgactg gataacctta atgcagcgcg aattggcagc tcccgcgcta
    21541 ttacgcaact cattctcaat ctggtagaag gttcaccaat taagctaatt gatggcggaa
    21601 aacaaaaacg ctgctttact gatatccggg acggtatcga ggcgttgtac cacattatcg
    21661 aaaatgcggg aaatcgctgc gacggcgaaa ttatcaacat tggcaatcct gagaacgaag
    21721 cgagcattga ggaactgggc gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc
    21781 atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag tagctgctac tacggcaaag
    21841 gatatcagga cgtagagcat cgtaaaccga gcatccgcaa tgcccaccgc tgcctggact
    21901 gggagccgaa aattgatatg caggaaacca tcgacgaaac gctggatttc ttcctgcgca
    21961 ccgttgatct tacggataaa ccatcatgac caaagtaggc ttacgcattg atgtcgatgc
    22021 ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa atcttgagta agcataatat
    22081 tcaggccagc atttttttca gcgtcggccc ggacaatatg ggccgccatc tctggcgact
    22141 ggtgaagcca cagtttttgt ggaagatgct gcgctcaaac gcggcatcgc tttatggctg
    22201 ggatatttta ctggcaggta cggcctggcc aggtaaagag attggtcatg ccaatgccga
    22261 tatcattcgt gaagcggcta aacatcacga agtcggtctg cacgcctggg atcaccatgc
    22321 ctggcaagcc cgtagcggta actgggatcg gcaaacaatg atcgacgata ttgcacgcgg
    22381 tcttcgcact ctggaagaga ttatcggtca accggtaacc tgttctgccg ctgcgggctg
    22441 gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc catttgcgct acaacagcga
    22501 ttgtcgtggg gccataccgt tccgtccact gcttgaatca ggaaaccctg gcactgcgca
    22561 aattccggtg accttaccca cctgggatga agtgattggt cgggatgtga aagcagaaga
    22621 ttttaacggt tggttactca accgcatcct gcgagataaa ggcacgccgg tttataccat
    22681 tcatgcagaa gttgaaggct gcgcttatca gcataatttt gtggatctcc tcaaacgcgc
    22741 agctcaggaa ggcgtgacat tttgcccttt aagcgaactg ttatcagaga cgttgccgct
    22801 cggacaagtt gttcgcggaa atattgccgg acgtgaaggc tggctgggtt gccaacaaat
    22861 tgcgggtagt cgctgatgaa atcggtacgt taccttatcg gcatcttcgc gtttattgcc
    22921 tgctattacc tgttaccgat cagcacgcgt ctgctctggc aacccgatga aacgcgttat
    22981 gcggaaatca gtcgagaaat gctggcatcc ggcgactgga ttgtgcccca tctgttaggg
    23041 ctacgttatt tcgaaaaacc cattgccgga tactggatta acagcattgg gcaatggcta
    23101 tttggcgcga ataactttgg tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc
    23161 gcgctggtga cctggtttac tctgcgctta tggcgcgata aacgtctggc tctactcgcc
    23221 acagtaattt atctctcatt gtttattgtc tatgccatcg gcacttatgc cgtgctcgat
    23281 ccgtttattg cattctggct ggtggcggga atgtgcagct tctggctggc aatgcaggca
    23341 cagacgtgga aaggcaaaag cgcaggattt ttactgctgg gaatcacctg cggcatgggg
    23401 gtgatgacca aaggttttct cgcccttgcc gtgccggtat taagcgtgct gccatgggta
    23461 gcgacgcaaa aacgctggaa agatctcttt atttacggct ggctggcggt tatcagttgc
    23521 gtactgacgg ttctcccctg gggactggcg atagcgcagc gggagcctga cttctggcac
    23581 tattttttct gggttgagca tattcaacgc tttgcactag atgatgccca acatagagct
    23641 ccgttctggt actacgtgcc ggtcatcatt gccggtagcc tgccgtggct gggattactc
    23701 cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt ccgcaaccgt ctatttgttg
    23761 agctggacga taatgccgct gctgtttttc tccgtcgcta aaggcaaatt gcccacctat
    23821 attctttctt gctttgcacc tctggcaatg ctgttggcgc attacgcgtt gctggcagca
    23881 aaaaataatc ctctggcgct gcggattaat ggctggatta acatcgcttt tggcgtcact
    23941 ggcattattg ccacatttgt gatctccccc tggggaccaa tgaacacgcc ggtgtggcaa
    24001 accttcgaga gctataaagt cttttgtgcc tggtcgattt tttcgctatg ggcatttttc
    24061 ggctggtaca ccttaacaaa cgtcgaaaag acctggtctt tcgccgcgct ttgcccgctg
    24121 gggctggcgt tgctggtagg attttcaatt cctgacagag tcatggaagg aaaacatccg
    24181 caattttttg tcgagatgac acaagaatca cttcagccaa gccgctatat tcttactgat
    24241 agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac gcgatgacat catcatgtat
    24301 cgccagacag gtgagttgaa atacggcctt aattatccgg atgcgaaagg gagatttgtc
    24361 agcggtgatg agttcgcaaa ctggcttaat caacatcgtc aggaggggat tattacactc
    24421 gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg ccattccgcc cgcagatgcc
    24481 atcgttcgtc aggagcgtct ggtgctgatt cagtatcgtc ccaaatgatc tggctaacat
    24541 tagtctttgc cagcttgctt agcgttgccg ggcagttgtg tcagaaacag gcaacctgct
    24601 ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg gcgctggctt
    24661 gtcttggtct tgccatgatg ctctggctgc tggtcttgca gaacgtaccg gtaggcattg
    24721 cctacccgat gttaagtctg aattttgtct gggtgacgct ggctgcagta aaactgtggc
    24781 acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt cattattggt ggcattgtga
    24841 tcctcgggag tacggtgtaa tgtgcctgat atggggatta ttcagcgtca ttattgcctc
    24901 agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg ccgccgatga cgcacttatg
    24961 ggattttatc gcggctctgt tagcttttgg cctggatgcc cggatactgt tactcgggct
    25021 gctgggttat ctgctttccg ttttttgttg gtataaaacg ctacataaac ttgcgctaag
    25081 caaagcctat gccttgctca gtatgagtta tgtgctggta tggattgctt caatggtact
    25141 tcccggctgg gaagggactt tttcgctgaa agcactactg ggagtagcct gtattatgag
    25201 cgggttgatg ctgattttcc tgcccacgac aaaacaacgt tactgagttt tccctgccgc
    25261 tgtacactgc cgttcccact catcggggct ataactgctt gccgagagga ctttcaccgt
    25321 atgcagcttt tcacgattaa tacaataaag cgcattctgc aaaggcgaga gtaaatcccc
    25381 cactttcacg gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc cgccagcatc
    25441 gcagagcata agtacatgtc tgttatcttg tttattgcag cacgattttt tgtgccagcc
    25501 attccattgc attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata
    25561 aactctttat caacggcaat ggaatggcag aaatgagaag tgaaacggag ggttaattat
    25621 tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc
    25681 agctccggcg gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag cttatctttc
    25741 acccattcgc caagatcaac ggtttgctgg tcatactcca ccaccgctac cggccgatga
    25801 ccaaactcct tgtcggcaac ggaaacgata aacacctgca aaaccgcagg atgtgcagca
    25861 ataacacgct cgacttcttc cggctgaata ccctcaccgc cactgaagaa cagattgtct
    25921 aaacgtccga caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac
    25981 cagccttcgt cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata
    26041 ctggcagccc gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc
    26101 aaaccaacgt ctgccaggcc gtcggcttct ttcgcacata ccgtggaggc aaactcggtc
    26161 agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc
    26221 gggatagccg cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc
    26281 aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc
    26341 aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt
    26401 ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac
    26461 ggaatcagcg ataacacacc ttgcgcactg gcaagatggg cctgataagt atggacagcg
    26521 gctttcggca aaccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc
    26581 cacgcagcgg catgtacgcc ttcaaccagc tgaatgtgca gcgacgttaa tgccggaaac
    26641 gtgttttccc catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca
    26701 agcaacggtt gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac
    26761 gccagccagg cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc
    26821 tcaaccaccc cctgcgccgc aaagcccgta gctaactcat cgacgcgagc acaaagctcg
    26881 cgccagttga gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc
    26941 cagtgacgcc acggccagtc agagaagatc ataacaactg ctcctgtaca tcagcgtcca
    27001 ctaacggcag tgggctcccc ggccagcgac gaacctgctg cgcctgcatc agatccagcg
    27061 tgtccagtcc cggaatggtg tctggcgtta accaggcagc aatccgcgcc agttgcgtta
    27121 gacctaagct cgactcaatg gaagaactga tcaccgccgt cagccccagc gcatgcgccg
    27181 cctgtacctg ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg ataactaccg
    27241 cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg
    27301 caatggcaat gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct
    27361 cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctgac
    27421 ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct
    27481 ccagcaacag attcaccacc atgccgtcgc gcaccgcttc gtacaatccc actttgacct
    27541 tcgccacttt ctcgcccggc atatcggcaa gtttgaggat cagatcgtcc ggatcgccat
    27601 tacacagcgg tgccgcacgg tagttggctg cttgcggcaa cgtatctgtc agttctgcca
    27661 atgcacagct tacgccaaag gccacggaag gcatctgcgg tagctcgcaa tcgcctgcca
    27721 gccagttatt tacccaggca agcagcacgc tttgcgcctc ttcccagttt tcctggctga
    27781 agcccggcag tggggagatc tccccccacc cttcgcgctc gccctcacgc aggcaaacgt
    27841 acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac cacccccgcg tccatgggga
    27901 tctgccagcg gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc
    27961 aggctgacgt ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa
    28021 cagcatggtg gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt
    28081 cagcgcagct ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca
    28141 acggacggtt tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc
    28201 cagcgcctgt tttgcgtcgt actgacggca caggaaccag atttctcgcg cttttttctg
    28261 cccgacgata cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg
    28321 gccagtctga ccgaagatgg cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag
    28381 aacgtgacca ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt
    28441 acggatctga cgctggaagt ccagcacgtt caggtgatgt acgccggaat catctttata
    28501 gccgccgtaa tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc
    28561 gccagtcaga atgatcacgc cgatattgtc gtcataacgc gcatctgcca gtgcctggat
    28621 catctctttt accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat
    28681 ttttgcgata ccgtcggtgg atttttcata acgaatgtcc tcgaaacctt cggagcagtc
    28741 gtgccattca accggtgcgt aaggcattgc ttcatcagga taaatcatat tgtgtccttt
    28801 agtcagaaac gcaagatctg cgccagactt gcgattacgc cagcgggatt ttcccgatgc
    28861 gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccagggcg
    28921 cggaatttgc tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg
    28981 ttagcacgta aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt
    29041 gcgccattat tgttgctgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag
    29101 gcaaaaacag gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa
    29161 cgctgcgccc attggcgatc ggaacgctga cgttccgcac gttgttcagc attttgcagc
    29221 cccggatgcc cgccttcgac aataacccca caaagccccg ccagcccctg gcaagccgcc
    29281 atcatcgcca cccgtccgcc aagcgagtac cccaccagcc agaagtcaag gatgttgtaa
    29341 ctaaccaagg ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc
    29401 gccgaaccac catgacctag gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg
    29461 cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt
    29521 aaacctagtt ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca
    29581 gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac caccatttca atcaccgtgg
    29641 tggttggcgt gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag ttttgcggat
    29701 gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca
    29761 gatagaaacg ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt
    29821 tgttgttcac cacaattaat accagcggcg cagaaacctg acgcaataac gccagcgcgt
    29881 tgagatcgta aagcgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc
    29941 gctgaacgcc tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct cggttgctgt
    30001 acaccgggta acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt
    30061 taccaacaaa caattgcccc tgttcaggca gatagtcgct gatgcgatgc gccagttgcg
    30121 cttcgccaaa cgcatcacgg cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg
    30181 ggatttcaac gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa
    30241 tattggcaat taagcgacgt ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat
    30301 caacaatcca gtactcttct ggttcacagc ttgcctgcca ttgcaggagc cgtttccccg
    30361 tcaggctact tcccagttgc accacaattt gcgcctgctg cagctcgctg gtcgctttgg
    30421 cattgcctaa ccaaagatcg gcacacggca gcggctgccc ggtttgtgac agcacatcgc
    30481 caatcagcgg ccagccaaga gtttgcgccc acagggcaac ttttttgccc tcttccgcac
    30541 tcatgcgccc ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt
    30601 tttcactttc cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcac
    30661 ccagacgctg ttgccagcta agcccggtat cgtccatttc gccatacagc ggttcagcaa
    30721 acgggcagtt gatatggact ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag
    30781 aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg
    30841 ggtgagaggc gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct
    30901 ccggcgggcg atcggcggtt aagagaatca gtttttctcc ggttaacccg gcttcaatca
    30961 gtgccggata gagatttgcc accgccgtgc cggaggtcac aatcaccgcc accggctgct
    31021 tgctgacttt cgccagcccc agcgccagat gccccaaccc acgctcatcg aaatgggtgt
    31081 ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagcctg
    31141 gggcgataca gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc
    31201 agcgtcggtt aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt
    31261 ataaggagct acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat
    31321 tccatttgta ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc
    31381 tcggggtcgg aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta
    31441 atttttgctg agcgcaggga aacgcagaat tcgctttgtt gtaatgagag atagcccgca
    31501 gaaccggcgt accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga
    31561 tcgcgcggta agccagcaac tgctgccgtt ggcagcaact gatgtaaaca gatcacatca
    31621 tccgctttgt tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc
    31681 agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg ttggcagata
    31741 tcttccacca ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag ccactctcct
    31801 aactgctgcg cctgcttatc atcaggatga tttgctactg ttcccgccag cgcttcagta
    31861 cgcagcgctt tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt
    31921 tcgccatcaa aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc
    31981 atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc
    32041 actttgtcga gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag
    32101 cccgttttgt ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc
    32161 ttgatactca ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg ctgaagggag
    32221 ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg
    32281 cggggtaaaa gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag
    32341 tcagcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg
    32401 gtaatcgcgc ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc
    32461 gggtacgttt gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa
    32521 ggaatatcga taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt
    32581 agattttcca gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac
    32641 attatacggg gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt
    32701 gccagcaaca gtcctagtac cagcccgacg gccgcgccca caccaattcc ttgccagggt
    32761 ttttcgtgga cgtagtcatc agcacgataa acagcctgct tcgcccgata gtaataactg
    32821 tctgaagcct ggctaacccg ttttttcaca tcatccagcg ctttttctgc atgcgctttc
    32881 agctcaacat atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt
    32941 tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc
    33001 gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa gagttgttga ttacgcctga
    33061 attacctcgc gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca
    33121 gggataaaac cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca
    33181 ggcttatcag gccagtgatg cgtacatgtt tcgagtgttt tactcatcag ctgctgcccc
    33241 actttttcac cacgcaacgc ttcactgaca atcacccgac ctataacgac cggctcaaga
    33301 tcatcatcac ttttcagaat cctcgcatac gccaccagtt catcattttt ccaaccgaga
    33361 atatggcgat tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc
    33421 tcgacaacaa acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat
    33481 tcagagtgat gcagatcttg ccattcaatc ataatgctct ccttttagat gtatgcctta
    33541 tactagccgg aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact
    33601 tcagccggtg tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat
    33661 ccgacccaga gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg
    33721 cataccgcct ttaaccctgg aaaactggac aagattttta tcagtcacct tcatggcgat
    33781 catctttttg gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc
    33841 ttaacgattt atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc
    33901 ggctcatgga ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat
    33961 gatggcctgc gcaaagtaac cgcttatccg ctggaacacc cactggaatg ttatggctat
    34021 cgtattgaag aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc
    34081 gtgccgcctg gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat
    34141 ggaaggcaga ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct
    34201 attttcggcg ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc
    34261 atggtgcacg aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat
    34321 agctctacac gccaggctgc gacactagcc cgtgaggctg gagtcggcaa gctaatcatt
    34381 acccacgtca gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg
    34441 tcaattttcc cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactctgc
    34501 ttattattaa aaacagggtg aaacttgccc tgttatagca acccgcgcta catatatcca
    34561 cccacacagg catattgagc gatttttgat atttattcat ctccttacgc actatattca
    34621 gagcaataag ttgcctcttt tcatcttgat tcagcacaac ctttgcaact aaaaggagcc
    34681 catgatggtt acagttgtca gcaattattg tcaattatct caaacccaac tcagtcagac
    34741 atttgcagaa aaatttactg tgaccgagga attactgcag tctttaaaaa aaacagcgtt
    34801 atccggagat gaagaaagta ttgagttact gcataatatt gcgttaggtt atgatgaatt
    34861 tggaaaaaaa gctgggtaat gctgccaact tactgattta gtg
//
LOCUS       DAZXUL010000036        34791 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZXUL010000036 DAZXUL010000000
VERSION     DAZXUL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34791
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.036"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..2098
                     /locus_tag="ACH3L1_001704"
     CDS             <1..2098
                     /locus_tag="ACH3L1_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFT4076471.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="ACH3L1_001705"
     CDS             2095..3183
                     /locus_tag="ACH3L1_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFT4076472.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACH3L1_001706"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACH3L1_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFT4076473.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="ACH3L1_001707"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="ACH3L1_001707"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFT4076474.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="ACH3L1_001708"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="ACH3L1_001708"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFT4076475.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="ACH3L1_001709"
     CDS             8051..8602
                     /locus_tag="ACH3L1_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFT4076476.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACH3L1_001710"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACH3L1_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFT4076477.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACH3L1_001711"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACH3L1_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFT4076478.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="ACH3L1_001712"
     CDS             complement(11683..12681)
                     /locus_tag="ACH3L1_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFT4076479.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="ACH3L1_001713"
     CDS             complement(12760..13452)
                     /locus_tag="ACH3L1_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFT4076480.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="ACH3L1_001714"
     CDS             complement(13529..14032)
                     /locus_tag="ACH3L1_001714"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFT4076481.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="ACH3L1_001715"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="ACH3L1_001715"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFT4076482.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="ACH3L1_001716"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="ACH3L1_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFT4076483.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="ACH3L1_001717"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="ACH3L1_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFT4076484.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACH3L1_001718"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACH3L1_001718"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFT4076485.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACH3L1_001719"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACH3L1_001719"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFT4076486.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACH3L1_001720"
                     /pseudo
     CDS             complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACH3L1_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(22127..23560)
                     /locus_tag="ACH3L1_001721"
     CDS             complement(22127..23560)
                     /locus_tag="ACH3L1_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFT4076487.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="ACH3L1_001722"
     CDS             complement(23623..24072)
                     /locus_tag="ACH3L1_001722"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFT4076488.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACH3L1_001723"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACH3L1_001723"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFT4076489.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACH3L1_001724"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACH3L1_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFT4076490.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="ACH3L1_001725"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="ACH3L1_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACH3L1_001726"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACH3L1_001726"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFT4076491.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="ACH3L1_001727"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="ACH3L1_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="ACH3L1_001728"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="ACH3L1_001728"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFT4076492.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="ACH3L1_001729"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="ACH3L1_001729"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
     gene            complement(34082..34157)
                     /locus_tag="ACH3L1_001730"
     tRNA            complement(34082..34157)
                     /locus_tag="ACH3L1_001730"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="ACH3L1_001731"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="ACH3L1_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8486 a         8945 c         9142 g         8218 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataaaa a
//
LOCUS       DAZXUL010000037        34641 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZXUL010000037 DAZXUL010000000
VERSION     DAZXUL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34641
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.037"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1100)
                     /locus_tag="ACH3L1_001732"
     CDS             complement(<1..1100)
                     /locus_tag="ACH3L1_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFT4076493.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="ACH3L1_001733"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="ACH3L1_001733"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFT4076494.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="ACH3L1_001734"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="ACH3L1_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFT4076495.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="ACH3L1_001735"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="ACH3L1_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFT4076496.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="ACH3L1_001736"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="ACH3L1_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFT4076497.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="ACH3L1_001737"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="ACH3L1_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFT4076498.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="ACH3L1_001738"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="ACH3L1_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFT4076499.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="ACH3L1_001739"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="ACH3L1_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFT4076500.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="ACH3L1_001740"
     CDS             complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="ACH3L1_001740"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFT4076501.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSVGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="ACH3L1_001741"
     CDS             complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="ACH3L1_001741"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFT4076502.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="ACH3L1_001742"
     CDS             complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="ACH3L1_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFT4076503.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="ACH3L1_001743"
     CDS             complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="ACH3L1_001743"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFT4076504.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10283..11701
                     /gene="puuA"
                     /locus_tag="ACH3L1_001744"
     CDS             10283..11701
                     /gene="puuA"
                     /locus_tag="ACH3L1_001744"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFT4076505.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11850..12014
                     /locus_tag="ACH3L1_001745"
     CDS             11850..12014
                     /locus_tag="ACH3L1_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076506.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12004..13389
                     /gene="puuP"
                     /locus_tag="ACH3L1_001746"
     CDS             12004..13389
                     /gene="puuP"
                     /locus_tag="ACH3L1_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFT4076507.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13523..13768
                     /locus_tag="ACH3L1_001747"
     CDS             13523..13768
                     /locus_tag="ACH3L1_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFT4076508.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14081..15724
                     /gene="sapA"
                     /locus_tag="ACH3L1_001748"
     CDS             14081..15724
                     /gene="sapA"
                     /locus_tag="ACH3L1_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFT4076509.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15721..16686
                     /gene="sapB"
                     /locus_tag="ACH3L1_001749"
     CDS             15721..16686
                     /gene="sapB"
                     /locus_tag="ACH3L1_001749"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFT4076510.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16673..17563
                     /gene="sapC"
                     /locus_tag="ACH3L1_001750"
     CDS             16673..17563
                     /gene="sapC"
                     /locus_tag="ACH3L1_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFT4076511.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17563..18555
                     /gene="sapD"
                     /locus_tag="ACH3L1_001751"
     CDS             17563..18555
                     /gene="sapD"
                     /locus_tag="ACH3L1_001751"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFT4076512.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18557..19363
                     /gene="sapF"
                     /locus_tag="ACH3L1_001752"
     CDS             18557..19363
                     /gene="sapF"
                     /locus_tag="ACH3L1_001752"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFT4076513.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19431..19784
                     /locus_tag="ACH3L1_001753"
     CDS             19431..19784
                     /locus_tag="ACH3L1_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFT4076514.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20152..20940
                     /gene="fabI"
                     /locus_tag="ACH3L1_001754"
     CDS             20152..20940
                     /gene="fabI"
                     /locus_tag="ACH3L1_001754"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFT4076515.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21084..22211
                     /locus_tag="ACH3L1_001755"
     CDS             21084..22211
                     /locus_tag="ACH3L1_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFT4076516.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22279..24213
                     /gene="rnb"
                     /locus_tag="ACH3L1_001756"
     CDS             22279..24213
                     /gene="rnb"
                     /locus_tag="ACH3L1_001756"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFT4076517.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24291..24394)
                     /locus_tag="ACH3L1_001757"
                     /pseudo
     CDS             complement(24291..24394)
                     /locus_tag="ACH3L1_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24448..26422
                     /gene="pdeR"
                     /locus_tag="ACH3L1_001758"
                     /pseudo
     CDS             24448..26422
                     /gene="pdeR"
                     /locus_tag="ACH3L1_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(26404..26532)
                     /gene="ymiD"
                     /locus_tag="ACH3L1_001759"
     CDS             complement(26404..26532)
                     /gene="ymiD"
                     /locus_tag="ACH3L1_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFT4076518.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            26570..26743
                     /gene="yciZ"
                     /locus_tag="ACH3L1_001760"
     CDS             26570..26743
                     /gene="yciZ"
                     /locus_tag="ACH3L1_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFT4076519.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26833..27582
                     /locus_tag="ACH3L1_001761"
     CDS             26833..27582
                     /locus_tag="ACH3L1_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFT4076520.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27851..28069
                     /gene="osmB"
                     /locus_tag="ACH3L1_001762"
     CDS             27851..28069
                     /gene="osmB"
                     /locus_tag="ACH3L1_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFT4076521.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="ACH3L1_001763"
     CDS             complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="ACH3L1_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFT4076522.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="ACH3L1_001764"
     CDS             complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="ACH3L1_001764"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFT4076523.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="ACH3L1_001765"
     CDS             complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="ACH3L1_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFT4076524.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="ACH3L1_001766"
     CDS             complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="ACH3L1_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFT4076525.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="ACH3L1_001767"
     CDS             complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="ACH3L1_001767"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFT4076526.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32018..32608
                     /gene="ribA"
                     /locus_tag="ACH3L1_001768"
     CDS             32018..32608
                     /gene="ribA"
                     /locus_tag="ACH3L1_001768"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFT4076527.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32672..>34641)
                     /gene="acnA"
                     /locus_tag="ACH3L1_001769"
     CDS             complement(32672..>34641)
                     /gene="acnA"
                     /locus_tag="ACH3L1_001769"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFT4076528.1"
                     /translation="MLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGV
                     VGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVE
                     LVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKAFAAS
                     NELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKA
                     VTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLP
                     DPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAS
                     EPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRS
                     DTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPD
                     SPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPD
                     SDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIH
                     RSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEV
                     VPCRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT         8166 a         9028 c         8749 g         8698 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc aacgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt acccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgcg cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
     8281 gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt agccggagaa
     8341 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
     8401 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
     8461 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcggttttca
     8521 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
     8581 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
     8641 gctgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc attgcgctta
     8701 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     8761 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     8821 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     8881 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8941 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     9001 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     9061 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     9121 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     9181 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     9241 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     9301 tagtggtcag agtcgctgtt tttcagcgat atggtgctgc caagcggtga taaagccctc
     9361 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
     9421 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     9481 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     9541 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     9601 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
     9661 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
     9721 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
     9781 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
     9841 gtgcggctgc acattgctgg gactactagg aagataaatg ccatcgagtt tcggcaaaag
     9901 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
     9961 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    10021 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    10081 cacaaaatgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    10141 ttttcaatct agcagtggat tttcattttt gcaaactcaa tttaacattt gacaaacatt
    10201 tggtttgcat acagattcga atgatggtca ttatatttta cgctttgata acgagcggaa
    10261 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    10321 gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg
    10381 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    10441 aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc
    10501 ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg gtcaggaaat
    10561 gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt ctgccgccga
    10621 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    10681 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt
    10741 cccggttgta gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg
    10801 ggatctgcaa ccgccctgcg cgcctggcac cgatgaccgc aatacacaaa gccaggttta
    10861 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg aactggcgca
    10921 gttgcagctg attccggcag atggcgcggt cgctgaggcc tcgccgggtc agtttgaaat
    10981 caacctttac catactgata acgtgctgga agcctgcgat gatgcgctgg cactaaaacg
    11041 cctagtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    11101 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    11161 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    11221 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    11281 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    11341 cgtcgccctg cgtattccgt gcggtgaccg tcataatcac cgcgtggaat atcacgtggc
    11401 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    11461 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    11521 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    11581 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    11641 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    11701 attccccttg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    11761 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    11821 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    11881 gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc
    11941 taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca
    12001 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    12061 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    12121 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    12181 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    12241 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    12301 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    12361 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    12421 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    12481 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    12541 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    12601 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    12661 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    12721 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    12781 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    12841 attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    12901 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12961 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    13021 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    13081 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    13141 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    13201 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    13261 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    13321 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    13381 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    13441 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    13501 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    13561 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    13621 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    13681 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    13741 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    13801 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    13861 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    13921 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13981 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    14041 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    14101 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    14161 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    14221 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    14281 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    14341 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    14401 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    14461 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    14521 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    14581 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    14641 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    14701 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    14761 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    14821 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    14881 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14941 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    15001 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    15061 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    15121 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    15181 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    15241 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    15301 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    15361 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    15421 cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    15481 caatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg
    15541 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattaccta ttctgccgct
    15601 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    15661 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    15721 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    15781 ttcgttggct tcagcttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    15841 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    15901 attaacggtc aaccaattgc cgaacagtta aaagagctct tcccggcgac gatagaactg
    15961 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    16021 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    16081 atcccggtgt tctggctggc gcttctgttg acactgtttt gttcactcac gctaggttgg
    16141 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    16201 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    16261 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    16321 ctgatgcgta tcagcactat tgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    16381 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    16441 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    16501 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    16561 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    16621 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    16681 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    16741 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcgggactgg ctgtactgtg
    16801 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    16861 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16921 cggacgtgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16981 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
    17041 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    17101 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    17161 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    17221 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    17281 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    17341 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    17401 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    17461 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    17521 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    17581 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    17641 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    17701 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    17761 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    17821 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    17881 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17941 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    18001 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    18061 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgaca
    18121 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    18181 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    18241 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    18301 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    18361 agcgcgatgc cgcataaaag tcgcctcaat actctacccg gcgctatccc attgctggaa
    18421 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    18481 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    18541 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    18601 ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
    18661 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    18721 actggcaaaa atgctggcgg gaatgataga acctaccagc ggcgagttat tgattgacga
    18781 tcatccgctg cattttggcg attattcctt ccgtagtcag cgtattcgca tgatttttca
    18841 ggatccttcg acctcgttga atccccgtca acgtatctca caaattctgg attttccact
    18901 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18961 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    19021 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    19081 tgaagccctc gcttcactgg atatgtcaat gcgttcgcag ttgattaatc tgatgctgga
    19141 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    19201 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    19261 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    19321 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    19381 ccagattatt cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    19441 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    19501 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    19561 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    19621 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    19681 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    19741 agattattct gcaggaactg aaccgccggt caccctcacc ctgaaagggc gagggggccg
    19801 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    19861 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19921 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttaggg
    19981 taaggggaaa agcgcccccc ctgccgcagc ctgctccggt cggacctggt aactatagct
    20041 actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac
    20101 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    20161 ctttccggta agcgcattct ggtaaccggt gttgccagca aactgtccat cgcctacggt
    20221 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    20281 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    20341 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    20401 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    20461 tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
    20521 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    20581 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    20641 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    20701 gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat caaagacttc
    20761 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtacagt tactattgaa
    20821 gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    20881 gtagtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga actgaaataa
    20941 tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
    21001 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
    21061 tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt
    21121 atcatgaaac gcaatccagt actacggagt atgacgttct gcctctggtc ccggaagccg
    21181 caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagagacg ctggccccct
    21241 tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gaccagctta
    21301 ccgtgacccg ttcacaaacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg
    21361 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
    21421 ccggacctga ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc
    21481 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
    21541 tcggtcttac tgtctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc
    21601 aggcgcggac aattgcgaca tttcaggctt atctcgggca tccggtacgc tggttacccg
    21661 ggctggcgat acaaaactac gccgacgcgt cactgtttgc tgatgaatca atacgctggc
    21721 gaagcagcta tgaggtggaa aaactacctg aagagcacac aaaaagttca actgcagaac
    21781 tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt ctcgaaaagt
    21841 tgttaaacag cacacgggtc aatgcacagc cggataatca gcttgcggcg ttgttatgcg
    21901 cgcgtataaa tggcagtcct gcttgttttt ccacctgtat ggattcatca aatgaatata
    21961 aaaaaatcag cacccttctg cgcaagggcg aaaatgaaat taaccgatgg gctgaccgtc
    22021 attctgttga gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
    22081 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
    22141 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
    22201 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
    22261 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
    22321 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
    22381 ttcctggaag tcgacgcaca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
    22441 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
    22501 ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat
    22561 gaccgtctga ccatcgttcc tgatcatcca cttttaaaag acgccattcc ttgccgcgca
    22621 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
    22681 catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt
    22741 gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga aaaagaagca
    22801 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgacctcg
    22861 ctggattttg tcaccatcga cagtgccagc actgaagata tggatgacgc ccttttcgct
    22921 aaggcgctgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
    22981 attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg
    23041 cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc
    23101 gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt
    23161 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac
    23221 caggtttctg actggctgga aaataccggt gactggaagc ctgaaagtga agcaattgcc
    23281 gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    23341 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    23401 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    23461 gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac
    23521 gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    23581 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    23641 gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt
    23701 gctgaaatta gctctgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc
    23761 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    23821 atcaaaggcg aaactgcgac gcgtccgcag gatgaaatca ctgtccaaat ggccgaacgt
    23881 cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg
    23941 aaagataaag ccgggaccgg cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc
    24001 ggcatgcgtg tgcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta
    24061 cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt
    24121 gaaacggttt acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    24181 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatgg
    24241 atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact
    24301 ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaaacaa
    24361 tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac
    24421 tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac
    24481 aactttctcg gatcgcacaa tccatactgg cggttgacgg aaagcagcga tgctttgcgc
    24541 ttttctacca ccaaaaccac agaacctgat cgtacattgc agttatctgc cgaacaggct
    24601 gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat
    24661 gaaagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc
    24721 aacgcgtctg catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc
    24781 tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga cagccggggg
    24841 aatatccaac gcttcaatcg gttatgtgaa gattacacag ggttgaaaga acacgacgtc
    24901 attgggtaaa gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc
    24961 aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca
    25021 cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaaa
    25081 aacgagattt ttttaatctg ttccggtacc gacattaccg aagagcgccg cactcaggag
    25141 cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg
    25201 caggagttaa tcgatcacgc tattaatcag gcagataaca ataaagttgg ggttgtgtat
    25261 cttgatttgg ataattttaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag
    25321 ttattacgcg acgtgtcatt ggctatttta agctgtctcg aacatgacca ggtgttggcg
    25381 cgtccaggtg gcgatgagtt tctggtactg gcatccaata cctcacaaag cgcgctggaa
    25441 gcaatggcat cacgaatttt gacccgctta cgcctcccct ttcgtattgg tttaattgaa
    25501 gtttatacca gctgttcagt aggtatttcg ctctctcccg aacatggttc ggacagcgcg
    25561 actattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa
    25621 ttttgcgttt ttactccaga aatgaatcag cgggtatttg aatatctctg gctggatacc
    25681 aacttgcgta aagcactgga aaacgatcag ttggttatcg aaaatcacct agcgtgtcga
    25741 agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc
    25801 gttggacttc atttcctacg ccgaagagtc agggctaatt gtgcctttag gccgttgggt
    25861 gattctcgat gtcgtacgcc aggtggcaaa gtggcgggat aaaggcataa acctgcgagt
    25921 ggcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
    25981 ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga cagagagttg
    26041 tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc aactaggtgc
    26101 gcaagtgcat ctggatgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
    26161 tccgatcgat gccatcaaac ttgaccaggt ttttgttcga gatattcaca aacaacctgt
    26221 ctcgcagtca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
    26281 cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
    26341 gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gctggtataa
    26401 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat atcccgtcat
    26461 tatatatcgt attcctggat gataattagc cgttgctgtt aaacacttct cttttagcgg
    26521 caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga tgtctgaatt
    26581 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
    26641 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
    26701 tgaatctacg ccagacatcc cgaagacacc gtgggagatt taacaatcgt gccttcaggt
    26761 tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta taacgttata
    26821 agaattacag cgatgaattc ccgacaacaa actattctac agatggtcat tgaccagggt
    26881 caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac cattcgccag
    26941 gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt tgcagtttcg
    27001 cttgatagtg atgacgtcga aacccgtatg atgagcaact atacgctgaa gcgtgaactc
    27061 gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca tctttatcga aaatggcagc
    27121 agcaatgccc tgctcgcccg gactctgggg gagcagaaga aaaatgtcac tatcatcacg
    27181 gtcagcagct acatcgcgca tttgctgaaa gacgcgcctt gtgaagtcat tttgctcggt
    27241 ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt tgacacgcca gtgcatccag
    27301 caggtacatt tcagcaaagc atttattggt attgatggct ggcaacctga aactggattc
    27361 accggtcgcg atatgatgcg caccgatgtg gtcaatgccg tgctggaaaa agagtgcgaa
    27421 gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc catcggtccc
    27481 gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga tctgatgcat
    27541 cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc cttctctacg
    27601 gatgatttgc agtttggcaa atcatccgct ctaagatgat tcctggttga taattaagac
    27661 tatttacctg ttattaacac tctcaagata taaaattatt atcagcgata taacaggaag
    27721 taattatcac ctgcgtgata taaccctgcg cgcgagcaga tttcacggaa taatttcacc
    27781 aggcttattc ttagctatta tagttataga gagcttactt ccgtgaatca taaattcagg
    27841 agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg caattacttt
    27901 ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gaccgcaaca ccgcaatcgg
    27961 cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt tgggtacatt
    28021 aggtggtgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat cgtaaataaa
    28081 tgtcgattat cgactcgcaa ttataatacg cttgattctg ttcagtacga aataactgaa
    28141 taatacacac agtaaaatat tcgaaagcca cggatatatc cgtggctttt tatgttaacc
    28201 gcctgcgagt tttaccttca tccctttcgc ttcaagtagt gattttaata aatcacgctt
    28261 atcgccctgg atttcaataa ctccatcttt aactgctccg ccgcagccgc attttttctt
    28321 cagttccgct gcaagttttg tcagttcggc atcatcgaga tcgataccgg taatcaggca
    28381 aacgccctta cctttacgtc cactggtctg acgctgaata cgcaccgctc catcgccttt
    28441 agggcgtact ggggccgctt tgggctcatc aatacgcccg gtttccgttg agtagaccag
    28501 acggctgttg gaatcactca tcatgcactc cgctgtaaag aggcgttgat cgctttcagt
    28561 gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa tcaccatata atcaacacca
    28621 gccgccaacg cctgttctgg cgtcataatg cggcgctggt caccagcttc actcccctgc
    28681 ggacgaatgc ccggtgtaac cagtttgaac tcctgaccga atacctgttt aaagcgcaca
    28741 gcttcctgag cagaacacac cacaccatca aggtcacatt tttgcgtcag tgctgccaga
    28801 cgttctgcat aatctgcagg tgacagtgtc acgccaagat cggccaggtc gctggcttcc
    28861 atgctggtca acactgtcac agcaatcaaa agcggtgcat ctttgccaaa cggaaccagt
    28921 gcctcacgcg ctgcggtcat catacgcgcc ccaccggagg catgaacatt caccatccac
    28981 acgcctaagt cagctgcagc agcgacagcg tgcgctgcag tgttggggat atcgtggaat
    29041 ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt cgcgcacaaa ctgtggccca
    29101 aacaatgtaa acatctcttt accgaccttc agacgacaat cgcgtgggtc gatcttgtcg
    29161 acaaaggaca gcgcgtcatc acaattatga taatcaaggg caacaaccac aggagaattc
    29221 gtaacggcgc gggaagaaga tgaagcagtt aacgtcatga ccagaccttc ttgatgatgg
    29281 gcaccgggag tggcgcgaaa caggtaaacg gcgagcattc tacccgtgaa aataaaaaat
    29341 taacagttgc gatttttccc ctagcaggct ttttctgctc cgtcgttgcc ggagcacaat
    29401 gggacatcaa taactagtat gttgtaacta aagtcggatt tttttaaaaa ttacaggcca
    29461 tcaagaccgc gaatcggttt aatagttgac caggcacgac aagacggaca atgccagtag
    29521 agggtgtatg cggtaaaacc acatttctgg cagctataac gaggcttact acgcaccttc
    29581 tcgccaacca tgtcacgcag caccatcaga ctctctttgg cgcgcccttc ttccgcttca
    29641 tttaagtggt aatccattag cttatggaat acacgcatgg tcggatgacg ctgaagctgg
    29701 cgtgtaatat agacctgtgc agcctcacta ccgtcgcgcg cttcgatgat atcagcaagc
    29761 atcaattcag catcggcacc ggtgttctct tccaccgcgc gttgcaggaa ttctgcccat
    29821 tcggcagttt tacccaactg ctgatagcag gtttgcaaca tttccagcgt ttcgctgacc
    29881 agttctctgt cctgggatat gacgcgttgc aaactttcga cggctttggc gtattctcct
    29941 ttcgccataa acacgcgtcc catcatgatg gatacgcggg cgctgttttt atctgccgcc
    30001 gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat atgctgcagg
    30061 gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc cagcttcacc
    30121 aggcgttcgg caacatcaat tgctttctgc cactcactgg tagcctggta gatttgtagc
    30181 aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt gaacatgtct
    30241 tccgcgcggt cgtataaccc ggcggccatg taatcacgcc ccagttgttg aatcgccaac
    30301 agacgctgtt cataggtcag cgaggcgctt tccattaggg tctgatggat gcgaatagcg
    30361 cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc ttcaacggtg
    30421 cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg ttgattacta
    30481 agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc atcttgcttg
    30541 ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac aggcaaaagc
    30601 agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg cggcactaca
    30661 gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg ttcagcgcgc
    30721 gccagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc gatagcaaac
    30781 cccgcagcaa acaataccgc cagcaatgtg gaaatacggt actccccttg cgctaacaga
    30841 taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat cacgaagatc
    30901 gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat gtggttagag
    30961 cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt cgcgccgtct
    31021 gcgaaatggc atccacgcgc attgttgatg atttaaggcc taaattcgga aaatcaacca
    31081 gcgttaactt tcttgttctc gttgtgcaat ttcgcgattt tcttccgcag gtggtgttaa
    31141 tggcccacaa attcgttgcg caagccacgt tgccaccgcc accagcgccc acgaaatcaa
    31201 cgtagctacc accagatcgc gtggccaatg catcccgagc agcaggcggc ttcccatgac
    31261 tcccgttgcc cagaccaaca agatagcaat ggttaacgtt cgccgacgcg gccacagcaa
    31321 accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag gaaaggcaaa
    31381 ccccgtctct ttctgccagt gtgaacgcaa atattgtgga atatttttct cttcagccaa
    31441 ctgttctttt actagatttc cgcgttctgc tcgctttaaa gtgtagaact catcaaccgg
    31501 aatatgatgt gttttttcca accagataac aaaaggtcgt ggttcctgta ctttgtcttt
    31561 gatccaggat ttaacgcctt gtccaacaag gattgccgcc gccagaatgg caaataatac
    31621 aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaaca aaatcaaatg
    31681 tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg cttttagtag
    31741 ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta ctggcatgac
    31801 aagcaatagt gcagctccca ctgcggtacg tcgggcaatc gaacgcatgg cctctccttt
    31861 ttgataagtc ccacaatcat aactgaaaat gccagttcca agaaaaattg acagatttgt
    31921 gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt tatggcaaaa
    31981 taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac gtgtggcaga
    32041 agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag aactggcaac
    32101 cggacacgat catgttgcgc tagtctatgg cgatatttcc gggcataccc cggtacttgc
    32161 gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct gcgattgtgg
    32221 cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta ttttgctgta
    32281 tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt acgcactgca
    32341 ggatcaaggt tacgataccg tagaggctaa ccaccagtta ggcttcgccg ctgatgagcg
    32401 cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag tccgcttgtt
    32461 aaccaataac ccgaaaaaag tcgaaattct gaccgaagca gggattaata ttgttgaacg
    32521 cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata ccaaagccga
    32581 gaaaatgggc catttgctga acaaataacc ctcttgcatc gtgtaattca tttgcttgcc
    32641 ggaagcaaaa taaccggcaa gcaaatactt gttacttcaa catattacga atgacataat
    32701 gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc ggtgtcgata cgacaacggc
    32761 agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt caccggaacc gtcgcgccag
    32821 gttgtagatt ttgcagatcg ccaatatcaa tcttctcttc cccggttagc cctaacgttt
    32881 tacgcgtcac gccttgcgga aattccagcg gcaggatgcc catgccaatt aaattcgaac
    32941 ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat tccaagcaga cgaggtcctt
    33001 ttgccgccca gtcacggctt gagcctgatc catactcttt cccggcaatc accgccagcg
    33061 gcgtttgctc ctgcttatag cgcatcgcag catcataaat agagactacg tcgctgtcag
    33121 gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg atgcgaatat
    33181 tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg taggagttaa
    33241 agtcttttcg ctcaacaccc cgaccttgta gatatcgacc cgctgggctg tcgggcttaa
    33301 tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt gcgaggatcc
    33361 gcgcaccgtg aatatcttcc actggtgctg gtgttgcctg catttcatca aagaaaggcg
    33421 ataagcgaat ataggttgag tcctcctgcc aaccgtaggt atcggatcgt gtgacgttaa
    33481 tttccttcca ctctgctgtg ccttcaaaaa cttctgcgta ctctttgcgg aacatttctg
    33541 tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata tctttcagat
    33601 aaaccggctc gcctttgcga tcatggccga taggctcaga agccaggttg atattcatat
    33661 ttcccgccag cgcataggca accaccagcg gtggcgaggc cagccagtta gttttaacca
    33721 gcggatggat acggccttca aagttacggt tgccggacag caccgcaccg acggttaaat
    33781 cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta ccaatacagg
    33841 tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt gtcagtttcg
    33901 cttttgccag ataatcagaa acgactttcg aacccggtgc caacgacgct ttgacccacg
    33961 gttgccgctt gaggccaaga gttacggctt ttttcgccag caagcctgcg gccatcagca
    34021 cacttgggtt agaggtgttg gtgcacgagg ttatcgcagc aatgaccaca gcgccatcag
    34081 gtaactgata ctgatgtccg ttcataacat aatcgactgg caggcgatct ttatgcgtgg
    34141 cattcacttt cagttcgtta ctggcggcaa atgcttttgg tacatcgggc agtgcaacgc
    34201 gatcctgtgg gcgttttggc cctgccaggc tcgcttcaac gtcattcata tccagttcta
    34261 acatactggt aaaaatcggt tcatcgcccg ggttacgcca catgccctgc gctttggcat
    34321 atttttcgac caactcgacc tgatcttcgc tgcgcccgct taaacgcatg taatcgaggg
    34381 ttactgcatc gattgggaag aagccacagg tggcaccata ttctggcgac atattggcaa
    34441 tggtggcgcg atccgccaac ggtagtgaat ccagaccatc accataaaat tcgacgaatt
    34501 tccccaccac gccatgtttg cgcagcattt gggtaacagt gagaaccagg tctgtggcgg
    34561 taataccttc acgtaatttt ccggtaagtt taaagcccac tacgtccggg ataagcatgg
    34621 aaaccggctg gcctaacatg g
//
LOCUS       DAZXUL010000038        33521 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZXUL010000038 DAZXUL010000000
VERSION     DAZXUL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33521
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.038"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="ACH3L1_001770"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="ACH3L1_001770"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFT4076529.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="ACH3L1_001771"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="ACH3L1_001771"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFT4076530.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACH3L1_001772"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACH3L1_001772"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFT4076531.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACH3L1_001773"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACH3L1_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFT4076532.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACH3L1_001774"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACH3L1_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFT4076533.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACH3L1_001775"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACH3L1_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFT4076534.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="ACH3L1_001776"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="ACH3L1_001776"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFT4076535.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="ACH3L1_001777"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="ACH3L1_001777"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFT4076536.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="ACH3L1_001778"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="ACH3L1_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFT4076537.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACH3L1_001779"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACH3L1_001779"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFT4076538.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACH3L1_001780"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACH3L1_001780"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFT4076539.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="ACH3L1_001781"
     CDS             8212..9075
                     /locus_tag="ACH3L1_001781"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFT4076540.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="ACH3L1_001782"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="ACH3L1_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFT4076541.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="ACH3L1_001783"
     CDS             10411..11028
                     /locus_tag="ACH3L1_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFT4076542.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACH3L1_001784"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACH3L1_001784"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFT4076543.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="ACH3L1_001785"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="ACH3L1_001785"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFT4076544.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACH3L1_001786"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACH3L1_001786"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFT4076545.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="ACH3L1_001787"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="ACH3L1_001787"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFT4076546.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="ACH3L1_001788"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="ACH3L1_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFT4076547.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="ACH3L1_001789"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="ACH3L1_001789"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFT4076548.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACH3L1_001790"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACH3L1_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFT4076549.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="ACH3L1_001791"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="ACH3L1_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFT4076550.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="ACH3L1_001792"
     CDS             21859..23568
                     /locus_tag="ACH3L1_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFT4076551.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACH3L1_001793"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACH3L1_001793"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFT4076552.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="ACH3L1_001794"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="ACH3L1_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="ACH3L1_001795"
     tRNA            27625..27719
                     /locus_tag="ACH3L1_001795"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="ACH3L1_001796"
     CDS             27991..29202
                     /locus_tag="ACH3L1_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4076553.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="ACH3L1_001797"
     CDS             29614..30657
                     /locus_tag="ACH3L1_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFT4076554.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..31600
                     /locus_tag="ACH3L1_001798"
                     /pseudo
     CDS             31172..31600
                     /locus_tag="ACH3L1_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31630..32781)
                     /locus_tag="ACH3L1_001799"
                     /pseudo
     CDS             complement(31630..32781)
                     /locus_tag="ACH3L1_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            33237..33494
                     /locus_tag="ACH3L1_001800"
     CDS             33237..33494
                     /locus_tag="ACH3L1_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076555.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
BASE COUNT         8400 a         8811 c         8259 g         8051 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg cacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
    31501 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
    31561 tgagattggg cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg
    31621 gacacggccc taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc
    31681 actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat
    31741 tatttcccgg ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa
    31801 gctttccaca caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt
    31861 gtgtcgcttc agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg
    31921 aacaatgacg ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc
    31981 cagttgtgct gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc
    32041 gatgactact gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc
    32101 ccacttctgg tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg
    32161 gcaggccatg tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc
    32221 cctgacgacg caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa
    32281 ggcggggagc accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc
    32341 aggtgagccg aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc
    32401 agccgctgcg ggcaacccga agtacacgac acatcgcttt gatgctgaac tctgcccgat
    32461 gattttcgat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg
    32521 gaggatggac agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc
    32581 ggccagttcg ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc
    32641 ataaagctga gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttc
    32701 agcgagtttc agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct
    32761 gattgatact ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt
    32821 gtccactatt ggtgggtaag atcaccctcc attaaaacta aaacgggcgc tttctgatat
    32881 atcatctgta agcgcagtaa taccattagt agcacctcgc gcattgatta ttgaatatta
    32941 taactggaat gttatatttc ttgttatgat gctgttcagt attataataa tgttattatc
    33001 accaaagata actcatgcaa atgagaaagt aatatgtcaa aacagtgcat cctttgtgac
    33061 caaagagttt atctgcggca ctatacttgt ctcattaata ctcagttctc tattttgcta
    33121 catatcaata tcaccatcaa ttttcatgca tacttatggc ctgagtactt ttagttactc
    33181 aattattttc tccttctcag ttttattttt cataatcgga aatcaattat caaagattga
    33241 aaaattatcc aacctatggt ttttattacc caatagccaa ttttatcgca ttacaaataa
    33301 atggttcaaa aacaggattt gccgcatcta ttacaggttt tactcaaaca ataagcgctg
    33361 gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg tgttggtccc ggaactactt
    33421 taggtcttgg ttcactaata acttcattac tgtcaatagc cgtaatcgcc attgcaggaa
    33481 ataataaaaa gtaagtgtac tctgactgcg tatgtatcta c
//
LOCUS       DAZXUL010000039        33419 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZXUL010000039 DAZXUL010000000
VERSION     DAZXUL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33419
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.039"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(211..1215)
                     /gene="istA"
                     /locus_tag="ACH3L1_001801"
     CDS             complement(211..1215)
                     /gene="istA"
                     /locus_tag="ACH3L1_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFT4076556.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFDNPVTLFVPLIMGC
                     "
     gene            1562..2371
                     /locus_tag="ACH3L1_001802"
                     /pseudo
     CDS             1562..2371
                     /locus_tag="ACH3L1_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2568..3347
                     /gene="ipaJ"
                     /locus_tag="ACH3L1_001803"
     CDS             2568..3347
                     /gene="ipaJ"
                     /locus_tag="ACH3L1_001803"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFT4076557.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(4294..5223)
                     /gene="virB"
                     /locus_tag="ACH3L1_001804"
     CDS             complement(4294..5223)
                     /gene="virB"
                     /locus_tag="ACH3L1_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFT4076558.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(5440..5676)
                     /locus_tag="ACH3L1_001805"
     CDS             complement(5440..5676)
                     /locus_tag="ACH3L1_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFT4076559.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(5680..7581)
                     /gene="ipaA"
                     /locus_tag="ACH3L1_001806"
     CDS             complement(5680..7581)
                     /gene="ipaA"
                     /locus_tag="ACH3L1_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFT4076560.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(7590..8588)
                     /gene="ipaD"
                     /locus_tag="ACH3L1_001807"
     CDS             complement(7590..8588)
                     /gene="ipaD"
                     /locus_tag="ACH3L1_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFT4076561.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(8639..9787)
                     /gene="ipaC"
                     /locus_tag="ACH3L1_001808"
     CDS             complement(8639..9787)
                     /gene="ipaC"
                     /locus_tag="ACH3L1_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFT4076562.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKPTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(9750..11492)
                     /gene="ipaB"
                     /locus_tag="ACH3L1_001809"
     CDS             complement(9750..11492)
                     /gene="ipaB"
                     /locus_tag="ACH3L1_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFT4076563.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(11498..11965)
                     /gene="ipgC"
                     /locus_tag="ACH3L1_001810"
     CDS             complement(11498..11965)
                     /gene="ipgC"
                     /locus_tag="ACH3L1_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFT4076564.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(12022..12648)
                     /gene="ipgB1"
                     /locus_tag="ACH3L1_001811"
     CDS             complement(12022..12648)
                     /gene="ipgB1"
                     /locus_tag="ACH3L1_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFT4076565.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(12664..13053)
                     /gene="ipgA"
                     /locus_tag="ACH3L1_001812"
     CDS             complement(12664..13053)
                     /gene="ipgA"
                     /locus_tag="ACH3L1_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFT4076566.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(13066..14550)
                     /gene="icsB"
                     /locus_tag="ACH3L1_001813"
     CDS             complement(13066..14550)
                     /gene="icsB"
                     /locus_tag="ACH3L1_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFT4076567.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            14864..16479
                     /locus_tag="ACH3L1_001814"
                     /pseudo
     CDS             14864..16479
                     /locus_tag="ACH3L1_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
     gene            16489..16851
                     /gene="ipgE"
                     /locus_tag="ACH3L1_001815"
     CDS             16489..16851
                     /gene="ipgE"
                     /locus_tag="ACH3L1_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFT4076568.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            16851..17309
                     /gene="ipgF"
                     /locus_tag="ACH3L1_001816"
     CDS             16851..17309
                     /gene="ipgF"
                     /locus_tag="ACH3L1_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFT4076569.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            17318..18433
                     /gene="mxiG"
                     /locus_tag="ACH3L1_001817"
     CDS             17318..18433
                     /gene="mxiG"
                     /locus_tag="ACH3L1_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFT4076570.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            18441..18692
                     /gene="mxiH"
                     /locus_tag="ACH3L1_001818"
     CDS             18441..18692
                     /gene="mxiH"
                     /locus_tag="ACH3L1_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFT4076571.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            18705..18998
                     /gene="mxiI"
                     /locus_tag="ACH3L1_001819"
     CDS             18705..18998
                     /gene="mxiI"
                     /locus_tag="ACH3L1_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFT4076572.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            19004..19729
                     /gene="mxiJ"
                     /locus_tag="ACH3L1_001820"
     CDS             19004..19729
                     /gene="mxiJ"
                     /locus_tag="ACH3L1_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFT4076573.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            19726..20253
                     /locus_tag="ACH3L1_001821"
     CDS             19726..20253
                     /locus_tag="ACH3L1_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFT4076574.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            20195..20890
                     /gene="mxiN"
                     /locus_tag="ACH3L1_001822"
     CDS             20195..20890
                     /gene="mxiN"
                     /locus_tag="ACH3L1_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFT4076575.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            20883..21290
                     /gene="mxiM"
                     /locus_tag="ACH3L1_001823"
     CDS             20883..21290
                     /gene="mxiM"
                     /locus_tag="ACH3L1_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HFT4076576.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            21274..21702
                     /gene="mxiM"
                     /locus_tag="ACH3L1_001824"
     CDS             21274..21702
                     /gene="mxiM"
                     /locus_tag="ACH3L1_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HFT4076577.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            21832..22464
                     /gene="mxiE"
                     /locus_tag="ACH3L1_001825"
     CDS             21832..22464
                     /gene="mxiE"
                     /locus_tag="ACH3L1_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFT4076578.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            22451..24151
                     /gene="mxiD"
                     /locus_tag="ACH3L1_001826"
     CDS             22451..24151
                     /gene="mxiD"
                     /locus_tag="ACH3L1_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFT4076579.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            24170..25237
                     /gene="mxiC"
                     /locus_tag="ACH3L1_001827"
     CDS             24170..25237
                     /gene="mxiC"
                     /locus_tag="ACH3L1_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFT4076580.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            25250..27310
                     /gene="mxiA"
                     /locus_tag="ACH3L1_001828"
                     /gene_synonym="virH"
     CDS             25250..27310
                     /gene="mxiA"
                     /locus_tag="ACH3L1_001828"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFT4076581.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            27323..27724
                     /gene="spa15"
                     /locus_tag="ACH3L1_001829"
                     /gene_synonym="spaK"
     CDS             27323..27724
                     /gene="spa15"
                     /locus_tag="ACH3L1_001829"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFT4076582.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            27728..29020
                     /gene="spaL"
                     /locus_tag="ACH3L1_001830"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             27728..29020
                     /gene="spaL"
                     /locus_tag="ACH3L1_001830"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFT4076583.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            29101..29451
                     /gene="spa13"
                     /locus_tag="ACH3L1_001831"
                     /gene_synonym="spaM"
     CDS             29101..29451
                     /gene="spa13"
                     /locus_tag="ACH3L1_001831"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFT4076584.1"
                     /translation="MYRDVEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
     gene            29438..30316
                     /gene="spa32"
                     /locus_tag="ACH3L1_001832"
     CDS             29438..30316
                     /gene="spa32"
                     /locus_tag="ACH3L1_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFT4076585.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            30310..31191
                     /gene="spa33"
                     /locus_tag="ACH3L1_001833"
                     /gene_synonym="spaO"
     CDS             30310..31191
                     /gene="spa33"
                     /locus_tag="ACH3L1_001833"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFT4076586.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            31196..31846
                     /gene="spa24"
                     /locus_tag="ACH3L1_001834"
                     /gene_synonym="spaP"
     CDS             31196..31846
                     /gene="spa24"
                     /locus_tag="ACH3L1_001834"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFT4076587.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            31861..32121
                     /gene="spaQ"
                     /locus_tag="ACH3L1_001835"
     CDS             31861..32121
                     /gene="spaQ"
                     /locus_tag="ACH3L1_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFT4076588.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            32121..32891
                     /gene="spa29"
                     /locus_tag="ACH3L1_001836"
                     /gene_synonym="spaR"
     CDS             32121..32891
                     /gene="spa29"
                     /locus_tag="ACH3L1_001836"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFT4076589.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            32898..>33419
                     /gene="spa40"
                     /locus_tag="ACH3L1_001837"
                     /gene_synonym="spaS"
     CDS             32898..>33419
                     /gene="spa40"
                     /locus_tag="ACH3L1_001837"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFT4076590.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIW"
BASE COUNT        10305 a         5009 c         6786 g        11319 t
ORIGIN      
        1 aatcgctgga ttcggtcatt ctgtctccct tgtatatggg ttaaccagat aaccacggag
       61 agcaggtacc ggcatccacg ttacagacgg tcccggcaaa agtgcctgac ctgaccccta
      121 ttagcggtta ccagcgcccc accagacccg gtgacatcac cccccggatc atggacgact
      181 gggggcagta atcatgccgg gtctggctgg ctaacacccc attataaggg gtacgaataa
      241 agtaacgggg ttgtcgaagc tcaccagatt ttcaccagga tgcacgtcat actctttttt
      301 ctccggaggc agtgccagca tggactgctg ctcttcgagc cagcgatcac agggacgggc
      361 ctggattgtt tcatgctttc gttggttagc gacatcgtgc agccagcgca gaccgtggcg
      421 gttggctgtt tcaacatcga cagtgctccc catcgggcgc aggcgagtca ttagtgggat
      481 gtaaaaactg ttacgggtgt actgcaccat ccgttccacc ttacctttag tctgtgccct
      541 gaaggggcga cacagtcggg gagagaagcc catctccttg ccgaactgcc acagcgaagg
      601 atggaaccgg tgctgaccgg tctgatatgc gtcacgttgc agaaccacag ttttcatatt
      661 gtcatacaac acttcgcgcg gcacaccacc aaagaagcgg aacgcattac gatggcaggt
      721 ctccagcgtg tcataacgca tattgtcagt gaattcgatg tacaacattc ggctgtatcc
      781 gggaacagca acgaacacgt gaagcggtga gcgaccatta cgcatagtgc cccagtcaac
      841 ctgcatctgt cgtccgggtt cagtttcgaa ccgaacggca ggctcctgct cctgaggaac
      901 cgagagagaa cgaatgaatg ccctgagaat ggtcattccg ccacgatatc cctggtctct
      961 gatctcacga gcgattaccg ttgccgggat tttgtaagga tgagcatcgg cgatgcgttg
     1021 acgaatataa tcccggtatt catccaggag tgaagcaaca gcaggtcgcg gcgtatattt
     1081 tggcggctca gattttgcct gcaaataacg tttaacggta ttgcgggaga gccccagttc
     1141 tctggcaatc gtccggctac tcattccctg cttgtgcagg attttaattt ccataactgt
     1201 ctcaaaagtg accataagct ctcctgaatc aggagagcag attaccccct ggatctgatt
     1261 tcaggcgttg ggtgtggatc actattgcac cgttcgtgac acttgttgac tacctgtgcg
     1321 gcatctggta tcaaggtggc gtgaagccgg ttcaggttct catgcggaac gttggaacct
     1381 atatcccccc gataccaaag gcgaaatgta agtgacaaaa tgcaatgaac acgttctccg
     1441 ttaactgagg agcatggtga taaataaagg taaatgcaga aggtgtagag tctcgcagat
     1501 ttctgacatc atagagccta acattagatg cataacgcta cagattgtag aatctctaca
     1561 ggtgaaatat acactggttg gcgtttatgt cgcaaaacat gtcattcagg ttcatctcac
     1621 caataaggat atgagtgaag tggaggataa gtgagtctgc tgtcagagtt ttctggtgta
     1681 tgtcagtaac tgcgaaccat gtctgcttga tatgggagct tatggaggct ctctgtacaa
     1741 ggttagggag ctaacaaagc agggataaaa agtccgcctg atgcaggcac ggttcgttaa
     1801 atcttttgcc attggtaaca aaatggtgcg atgaatgttc aggctatctt tatcttgatg
     1861 gtggttcagc tgtttggtaa agaaatcgct gtaacaatag aagaactgca ggcagtactg
     1921 gtcccacatc gtttgctcag tcaactggtg aagttccgta gcgcgtaaat taatgctttg
     1981 catgaaacgt tactggagtg gatgaaagaa tacctgcata tatcattact gcgttggatt
     2041 accagtacaa cagattgaat gaattggaca tgttgataga cggcattgaa aagcagctta
     2101 taagcatagt gcggcagagt taattggaga gcacttcgcc ttctgagtat taagtaagca
     2161 tggtggtacc tgcggatact atctattcac tgtgctcggt cggcagcctt actgaccaag
     2221 gagttggggg agttgataac aaagttgaaa aaacagctct caaccaaggt ggttattgta
     2281 acaatagcaa ataagctggt gtgacctgta tcacaataac cgattatgga cgtaaatacg
     2341 acaaaaatat gtcagtatca ggcggtatta attgctgatg ctgatggaca ttaactctta
     2401 ataaaagggt ggatgctgaa aggttgttat ggtgacggac gtgatgacga cggaaaagag
     2461 tatgaggttt tggatttaaa gttcgttcgg ggaataagga cccggtcagc taattctcat
     2521 agtagttcgc attgctggat gcaatactgt gctgcaatta ctcgtcgatg tcggaacaac
     2581 ggaagccttg caaacgggga tgtattcata caggtgttat gttatatggg gttttattgc
     2641 agggggctat accaagagaa tatatgatat ctcatcagac tgatgtgagg gtaaatgaga
     2701 atagagtaaa cgaacaaggt tgttttttgg cgaggaagca gatgtatgac aattcttgtg
     2761 gagctgccag tttactctgt gctgcaaagg aattaggggt tgataaaata ccacaataca
     2821 aagggtctat gtccgaaatg acaaggaaga gttctcttga tttggataat cgttgtgagc
     2881 gggatttata cttaataact agtggtaatt ataatccgag aatacacaaa gataatatcg
     2941 cggatgccgg ttattctatg cctgataaga tagtcatggc tacgaggctt cttggattga
     3001 atgcttatgt cgttgaagaa agtaacattt tttcgcaagt aattagtttt atttatcccg
     3061 atgcaaggga cttattaatt ggcatgggat gtaatatagt acatcaacga gatgtgttat
     3121 cttctaacca aagagttttg gaagctgttg ctgtatcctt tattggagtt cctgtcggcc
     3181 tccactgggt gttgtgtaga cctgatggaa gctatatgga tccagctgta ggagaaaatt
     3241 attcttgttt ttcaactatg gaattaggtg caagaagaag taattctaac tttataggat
     3301 atactaaaat aggtatttcc atagttataa ctaatgaggc tttgtaaatt gtcgtttttt
     3361 atctgtgaag gagcttgaat attttggggt ggggggcata acaaatgata ttactgagta
     3421 caaatttagc tatattgggc ggattagttg tgtgggaaga cgaaatgttt ctcttgaaat
     3481 aagtgaactc cctcaaggaa tgattgtgtg cttaaatatt taagtgctgg taagtttgag
     3541 aaggctgaca tattttgcag agcaaacagt gattgacttg gatgatattt ttattttagt
     3601 cactcgttgt aattggagta tgctttattt tatttgaaaa tagatgtatt ttatacccgc
     3661 aatttaaact gtaagcccta tgaggggtat ggatattata agttactctt tgaccaagca
     3721 taacttttgg ggttagaaaa ataaatctct tgttaataat taatatgatt ttaaaatcaa
     3781 acttgtttat aattataaaa taatgtaagt cggtcatttt atgggaagat taggagaata
     3841 aaacagtaat tatatttgag aaatctgcta atatattctt aaagcttttt tgatactgtg
     3901 aacgaagtct gttaatggtg gattttttgt ttatcaccat gtaaaaaaaa ttgagtagta
     3961 aaagcgttga ctgctaatcc tcgctgtttt tattgaaacg attaaaaata cgctgaatat
     4021 tttcggccag tcactcatat tcacataaaa ttttgaccat catgccgcat cccttagttg
     4081 cacaattcat tttcaatgat atgaatatta ctaatttcac cagataattt tctcgtggtc
     4141 agcagggtat gagttttcac cgtaatttta aggaaacaca gtgcgtgggg attgaataac
     4201 agatagccga cggaattacc ccaccggctg aaagtcaagt tcaacgaaga tttttattat
     4261 ctgaattggg cagtttacat cagtgttcga tgtttatgaa gacgatagat ggcgagaaat
     4321 tatatcccga atagcttcat ctatttctct ctgaactgtt ttgtttattt tagataatgt
     4381 aaatattaga gttctgtttg ttttattctc ttttcttttt atgtaagtcc gtttgtcttt
     4441 agaaataaat aacgagtcaa cttttagcga agggttttgc tttctgtttt tgcttttctt
     4501 tatgatgttt aaaatttctt ttttatatat gtccgggggc aaatttgtat caaggtcttt
     4561 tatttcttcc tttaatattg gtagtgtaga actaagagat tcattagcct tttcaagtcc
     4621 tttataataa ttgaataata tcttgtagtc attaaagtta agttctgacg cgattggaaa
     4681 tagagatatt atttcctgtg gaacgcttgc tgcctgaaag gcacgagtga ctttcgcgcg
     4741 agacagattc tcttttttgg ctatgtcttt ataggacatc cctgatactt tcagaaaatt
     4801 aagaccaata ccaagttctc ggatgctatg ctcttttgct gtttgtatat cgtttgctag
     4861 ttttctggca tcaagagtag atatatattc ttttgaatat agaacttcaa gatctgctcc
     4921 tgcatatatt gcagatgctc ttctacgagt gccatccaga atttcaattc taccatcaat
     4981 ctcccttcct attacaggga agaattgttg tagctttata gtttttatga tatcggctaa
     5041 tgattcttct gttagggatt cttgatcgcg tacattcgtt ttttggttga cgaaggttaa
     5101 atcttggatc cttttatgag gaatcttggc tttgataaag gaaactttat taccagaatg
     5161 gagtgtaaac tctttctttt ggccttcctt tatacttaac aagtcgttgc acaaatccac
     5221 catatcacac cctgtttatt catattgatt tatattgaca aaagttaaat gcagtgtgag
     5281 caagattgta tgtaactgtt gatgatagtc aacggtagtc ttaaaaatta ctattttaat
     5341 agctggattg atgcaattta tctttattgg ataaaagaat aatattcttg tggagctctg
     5401 atgtgagagc tccatagatg aaaaaatgct tatcaattac tatattgatt ttgtactatt
     5461 tttgactaca gaaataagat ctccaatagt aataatgtgt tctaatgtag attcatctat
     5521 acgtagattg aactcaaaac taatcatatc cagcatttca atcagagaaa gagaatcggc
     5581 agaaagatct tcaataaatt tagtttcttc agatagtttg ctgtacgcta taccatagca
     5641 aaaggccact attgataata ttttttcttt tatcatattt taatccttat tgatattctt
     5701 taatactttt gatagggaag tggttacatc ttttgctgct ttatatatat tattgttttc
     5761 agctgtaata tcattgttat tttttaaatc agatatatca tctgtaagta attctgcaga
     5821 ggtatcgtca atttttgaca ataccttgct gagggcgcta gatacttctt ttgccttttc
     5881 atagatcgca tgatttttat caattgtatc tgcgttggtt tctgttacgt agtttcttat
     5941 atttgcatct gtaactttta atttgttgtt tattctatgt tctgttgtgg attttgtaat
     6001 gtcctttgat ttttcctgta actctcgttc ttgtgttcct gatttagttc caatgagaga
     6061 tataagatta cttagggagt ttgttacttt ttttgaagct tcaaatatcg tttcccgtgt
     6121 taaatatgtg tctccatttc ttgataaaaa atcagtctga agtacttttg aatcagtgtt
     6181 tgagttgaat ttattgattt gcgaagctac tctatgagaa agagaagcgc tacttttagt
     6241 tgtatctgat gagttatcat tactgcttgt cactccttcc gggtggagag ggggggtggt
     6301 tgaagagttc tgtatgttca gaggcctgtg tccccgagaa agagagttat ttgcagatct
     6361 ttcttgacta ctcgtagtta acaggtctga acctattcca tccgaattat ctggagtatg
     6421 gttgaaagcc ttttctaaag ctgaattatt tgcaaatgtc tggacagtat ctgatataga
     6481 gtgtggtaga acggaagtta ctttttgaac tagtgctctt tgtggattta gtaatgagtt
     6541 tccgttacgg gataatagat cgtttgtctg agaagtagga gactgtgcat cattttctgg
     6601 gttctcgtga tagctattgt catatactct gttgtcaaaa actctattat cgtaagttct
     6661 attatcatta tttatatgat agtgaattac aggttgggat gttttctcat tggctccggg
     6721 ggttggtccg ccatctggca tatcttcggg aggtgtaggg aggccatctt ggataggatg
     6781 cgaatctttt atatcttcta aaatatcatt aaggactttt ctcaacagct caggaaacac
     6841 ggctactgta tttaagtctc ttgacagttt acctatacca aatcctttac tatcaatagt
     6901 atttttacgt tgattgcgca aactatcaaa ggcaagtttt gcctgtgcgt caactaaatc
     6961 atctagtttg agagatgaaa cagtttcatt acttaacttt aattcagcaa gaatattatt
     7021 cttgataaat gatgatactt cagtattgag gagtttttgt cccaatgata aagcttcacc
     7081 agatgtgtgt tgttgtgcta atagtgcaag tctgtgctca atgttttgtc gtaattgatg
     7141 gagtttctta ttctttgccg accatggtcc aaaggcagaa tcttgggata atttatcttt
     7201 tataattagt tccgaatact gtgcgacaat tttttgaagt cccttttcgt tttcaaaatt
     7261 aagaccattt tgatgtacta aggagtacaa aacatttgaa aattcgagaa tctcgctttt
     7321 ggcatacgca ttcattgagt tggaataaat gcttcttaac acatcttctt tccctgagga
     7381 agaaaataaa aaatcaagac atttctgatt aaaagacgtt gcaaagtttt ctttttcaga
     7441 cactgatgct ggtgttttta gagaagtttg cgaactatgg atatcggata ttttgctttt
     7501 taactcgctg tattctgttg atgatggtga agttgcctta tataagaatg ttggcgcttg
     7561 agtattattt acattatgca tggcgcacct cagaaatgga gaaaaagttt atctgtatct
     7621 gtacatgagc ttattgtact actcaaaacc tttactaaat tatcaaaaat actattggca
     7681 ttactgtatt tttgaactaa agtttgaaga ttatttttca ttgtttcatc ttcggcagag
     7741 aatccggcat tccatgcctg atattttgca ttatctagca caacctcgcc atttccacct
     7801 agattatcta agctttttaa catattgtct attggggtca tgtttatact gacaacatat
     7861 cccccgtttt tttgagatac cttgccgatt gttccaccta attctgtaag ccatttattt
     7921 gcttgttcct gactaacagt attatttgct ggatatagcg gtttatcttt atatttttcc
     7981 ttgagttctt ccaatgcctt tttaagcgag ttgacttgta atttcacgga gtttccgtcg
     8041 ttacctccgg gagagatcca gccggcaaga ctggaaagaa cagcgctaaa atcttgatac
     8101 atttgagtat atgaactaac ggcatgttca tatactttca gatactgttc attaatatca
     8161 ttgatggagt ttgcaatttt agcccacagt tctctatgag atatcatttg atctccatca
     8221 agctcggctt ctttcggagc tgaatgtaat aattctcttg cgtctttatt aattggatat
     8281 tcgtgcctgg aaagaatatc taatagttga gcggatttat ttacatccat gctaatctga
     8341 gatgaatgta atgctatttc ttctagcgat gttttagtca acgttttttg tgaaagctct
     8401 ttctttaatg cctgatttgt tgttctgata ttatgaaggg tgtcgttgag catagtaagg
     8461 gaacttacag gatgagaact ggtcgttgtt tttatatcag aattaactgt ttcggttgat
     8521 gaaccgttgg tattgtttgg actgaatgat gaggtggaaa tactattagt cagagttgtt
     8581 atattcatgg ttatttcctt acaggaagag ccatatatta ttatggctct tggatatatt
     8641 aagctcgaat gttaccagca atctgactgg ctgtcgaatt ctttgattgg ttgatgctgt
     8701 caattatatt caataatttt tgtattaatt gatttgtcgc ttgggatgct tctttagata
     8761 cttgggatgc ttcctctgct tgtttactgc tggcctgact gattagttgt tcttcttctt
     8821 caagagcaga tgcataacgc cctcctgatg tggatatatt accagcaacg gctgatgttt
     8881 ccattgttgc acggccaatg ttttgttttt gctgcgcaga aagggtattg agctcgtaag
     8941 ttcttctctg ggtgtcaatt ttatcctgca aagaaatatc gggagaacta agagaagttt
     9001 tatgttcagt tgacagggat atattatctg gcgccagttt atttttacct aaaaattttg
     9061 tgctagagtt agtttgtggt gaggtgatat ttgtatctat ttgtttattt aaccctaatt
     9121 tagaacctgc aagctctttt tcaagagatt gagcagtggc aaggttcttt cttaaggctc
     9181 ctttttggtc gctaatccct gaatgcgttt ttttggcacc gatacccgtt atacctactt
     9241 gtgtgactgc tccagtaatg cttgatgata gggctgccag gccttgccga acaatgttct
     9301 ctgcagctga ttttgtagca tcgaacgcaa tcaatgacaa ttgagagccc aatttagttt
     9361 ctgcagtgcg gagagcagaa agtagaacgg ctacactaat aattaaagaa acagcattag
     9421 aagaaagact ggaaatatcc agagtgttct ctggagtaag ggtgtgactc atctctgaaa
     9481 gatctttcat ctttttgtca gtaaggcgag caatgatttc tgaatcatgc tgaacctgct
     9541 ctgataactt taaaagttga tcatcattta atgtggttgt taatacggga tttttaccga
     9601 cattaagtgg aatatgcgct gcaatctgct gataattttg tgatttttgt gtttcggaag
     9661 aactttgagt ttgttttgtg gatatatctg tatataaaat ctgggttggt tttgtgtttt
     9721 gaatttccat aacattctcc ttatttgtat caagcagtag tttgttgcaa aattgctttt
     9781 gcaacatcag ttctattagc ctgagagttg gacattgagg ctaatagatc tgcaattact
     9841 tcctgcaatt ggccgaattt ttctattgct tcactgatat attttgacag ttgttcaact
     9901 tgatatttcg ataatgtcag gtctgctaga tttgtcgacg cgctgttctg gaaaacagca
     9961 gaagcgacac ttcctcccgc ttgtgtggca gagttaacac tttctcctaa taaaactgct
    10021 tggtttaaat gggtggaaat aatttgtttg gatattactt catcacccgc tgcaccaaga
    10081 aatttattta atctggcaac agcattagtg attaaatcgt ccagttgaga agaaaaattc
    10141 ttgagaaact ttggtataag gtctgtgagg gttttaccta ttattttgcc aatattttct
    10201 gcaagttttg ctgctgcctg tttaccaaca gtggctacga gaacgactgc tgcaactagg
    10261 acaagagcgc ctgcgattgc ccccagaata gagccaatca ttttggcttt tttcgagtcg
    10321 acgcccaagc cttcgagcat ttttgtaaat gcatctgaaa ggagtttgat taagggttca
    10381 atgactgctt tcatgatcgg attcagggct tgttccatga aggaattgcc ggtcgctgct
    10441 tgtactatag catccgtaac cataagagct aaaccaacag ctgccagtgc tagagaggct
    10501 cctccagaaa aagctgctgc aacaacacta acgatagtta aaagtgcccc aagtattttc
    10561 ccaacacaac ccattactct gttgagttct tctgctttac gtacttcagc agcatactca
    10621 tcagattttc tctccatttc agtttttctt gattcttgga gagactggaa tagagccaga
    10681 tcatttttta aagattcttc attatttttt ccaactagtt gaataaaggt tgccatcaat
    10741 tgagtaacac tggcaagtcc ggttaatgat ttctgctggg ttgatagctg ttcagcagat
    10801 gctgtgtttg aaaatgcaga aaaagagtct atttcttttt cgagttgcat tgatttatct
    10861 gtaagtttgc tatgaattga cagggttttc tgctcaatca atgtcctgtc tttaactgct
    10921 gcgtcttttt tgatagtgag ttgtatttct tcccggctta attttttctt ttctggtgac
    10981 tctgggtcga gttcggataa tcttgtttga atttggttaa ttttattttc taggtctttt
    11041 attttagaat ctgcgttttt tagtttatta atttgttttt catagtctct ggttagtcct
    11101 tcagtttcag atagaagagt gttaatttta tcggagaatt ctaggttttt ttgctgtctt
    11161 gcctgttgct gggacttcca agcagtaatt ttatttgtta atgcagttaa agatttttca
    11221 ccgagtattt gaataaggtt tccaattaaa agcgttagtt gggaacttgc atttaatgac
    11281 tttggtgctt taagttcagg gattaatata tttgaagttg agtgtgcatt agttgtatta
    11341 atattttggt tagcagtaag atcagcaatt gtaagagaaa ataatttgtt agctgcatca
    11401 tttgcagctt ggatagtatt gtctccaagc tcagtggaag ccaatatttt ggcaagagga
    11461 aaaccagtgg ttgtggtgct tacattatgc ataataatta ctccttgata tcctgaattg
    11521 cgtccaagta tgattgtgct tttattttta atttttcatc attgctgtgt tgaattacga
    11581 gttcgaagca ctctttagct tttaaggggg ctttcaaccg aagctggcat tgtccagtat
    11641 ggaatactgg tgtatagtca ttttttccta atgcaaaagc gacagcataa aggtctgctg
    11701 cttgttggaa ctgttctttt atctgataaa tagctgcgag tcccataatg tagtctacat
    11761 tgtaaaagtc gtatatacat aaaaacctga agaaaacttc agcttcctct attcttcctt
    11821 tgttgtaaaa gtcataagca tatgaataaa tgtcatccat catatcatca ggaattgcat
    11881 taatatcttt cagtgtagcg ccagagttaa ttgcatcaat tactgcagta gagatgcttt
    11941 cattttcggt gatatttaaa gacataaggt ctcctttttg tctgctttac tttaataagt
    12001 ataagattta atataaaaga tttaatttgt attgctttga cggtatacag cctttagaac
    12061 ctctgcgctt atttctattt gtaaaacttt tgttgattca ttaaatattt tatcgtagac
    12121 ggaactggat actagatttg taagatcctt catttgtgtg ttaaacaaat ccatgtcact
    12181 gctatagcgt tttaaaatct ttgctctgag gccagatgag gcaatctcgt ttagtgctgt
    12241 atgactgata gatgtttgtg ctgatttttc atcaagtttt atatccggga attttttttc
    12301 aatagctgca aatatgtttt ttttgttatc tgaggtcaaa acaactccat tgtgtatggc
    12361 catttcatct atacttctgt ttatcattga agagacataa gtggttcgct cttggctcat
    12421 ccaacaaaat agttgattcc ccgaattact atcttttatt ttgtctatga cgctgtccct
    12481 gattttattt atattcaccg ggcatccaaa attcttctga ggaaagcgtt gttttaagtt
    12541 aaaaacagat aatattttct ttacaagctt attgcgactt aacgaatcaa atgatgggcg
    12601 aggaatcgcg aattcaacct gtggaagtat tttgtttaga atttgcatga taccccctat
    12661 atgttagttc acttctgaag tgatgtttgc aatggttagt ttaatacaaa ataaaaattc
    12721 atacaaagat tcaatgagtt catcaggtga aagatatttt ttgtagtgtt tcttggtcaa
    12781 aacacattga tgagtatcag ggagaagaca gatagaagaa ccataatctc tactcaaaga
    12841 gtagttaagg atgagtatgt gttctggatt aataatattt tctgtaaaaa gttcatgttc
    12901 attaagaagt ccggtcaaaa agatgtattc atcattatct gttatacaaa caggtatttg
    12961 ttcttcgagc aatataaagg cttgattttt atcgttaaaa tcaagctttg cacctgttat
    13021 ttcataaagt ttatcatata gtttgcgaca catttctatg gccttctata tattagaatg
    13081 agagttattc aataaaagct tattgatctg taataagtcg tgaatgtaaa gtttggttcc
    13141 aagatctggc gatttaagag aattgtaata atcaataatt tttattgatt tttcgatgat
    13201 ttcttctttt ttatctgttg cttcggtttt taattcgttt aatacacgag tcatattgct
    13261 gggtgctgat tttactaagt atgagtggca agcgttgatg accgcatcaa agtcttctga
    13321 agatacattt tcagtaggat gtttgtaaag tgaaattgat aaaggcttga atgtttttgg
    13381 tgtgtggata aggttaattt tattgaaact tttaagatag ctgcgtcgta attgagtcaa
    13441 tctggaaaga tcaaaatcag attcaatata cttcattatt tcgttataag actgattgag
    13501 actttcaatg cgttgcctaa ctttatttgc atacgcgtat acatcgttag gtgtagcaac
    13561 caatttaacg tcagggagtg gaaagtatat ttcagaattt cctgctttaa gaacattaag
    13621 tgccatacca gcacagtttt cagatttact gattaattta taatttgcca attgtctgaa
    13681 agcatctgct ttctttttgc atataaattt tgacatgttt gtttcatcaa ggccaaaaag
    13741 gttatggctg attttaccat cctttgtttt attatcacct gataatggaa tgtatacttt
    13801 atcagcactg accccccaat aaccataggg gtcttttttt tgattagctc tgggaaaaaa
    13861 tatttttttg ttgttatttt gatcttgttt tgaatttaat aagtttctaa cctcttggcc
    13921 tgcatcaagt ctttcggctg tttggtctga aatcatattt agtttgtctt gcttataggt
    13981 atcgacactg taacctgagc gtttttcaaa aaggtagttt atttctgtat cttttgtaac
    14041 attcccgccg ggataccagc tgagataggt atgagttggt tcctgtagta aattgttttt
    14101 tatactgata ctagtatgcc ctaaatgggt tatgcctcta tttccccagc aacgaaaaac
    14161 taaatgtctt tcaagatttt tggctgtacc gatgccatga aaacgctctg cggtattcaa
    14221 cattaatttg atgtctctac ctgtgaaaga gtgttgaatt gagtttttag ctgcttcata
    14281 ggcggcaaac gcactttctg cagatttttc gctagctaat gtattgagga aaagcaataa
    14341 ggatttttga ttctctagtc gataattttt tagttgttca tttaatatct gtgaatttat
    14401 ctttgcatta atagtgctta atgttctgaa aaagagatcc tttaggttga atttctgtgc
    14461 tatcaatata ggtccatgct ctgtatcttc aacgcgtata ggcccttttg tattgcttgc
    14521 gtcaatgaaa ttgctaattt tgaggatcat actttattaa ctctccatta cttggtgatt
    14581 gtggggatgt aaaatgtatt tggtatataa tatattcaat taataaattt agaaacttga
    14641 gcctgttaac ataatcaaat tttcttttgc tgtacataat atgtacctcg tgagcatatg
    14701 tagtgctcgt ttcatcatga aatcccacaa gataaagtgc ctgatgtatc aggctcggag
    14761 tgttatagaa aaagagagaa ccctgttgaa taagtatctt ttttgttgta aaacgaggta
    14821 caaaactgca atcaatgccc catttatgac tgaggataat taaatgcaca taactaattt
    14881 gggattgcat caggtttcat ttcaaagcgg agattcctat aaaggcgcag aagaaaccgg
    14941 aaagcacaaa ggtgtaagcg tgatttcata tcaaagagtt aaaaatggag aaagaaataa
    15001 ggggatcgaa gcgcttaatc gattatattt acagaaccaa acaagcttaa caggaaaaag
    15061 ccttttgttt gcgcgtgaca gagctgaagt tttttgcgag gcaataaagt tggcaggtgg
    15121 tgatacgtca aaaattaaag ccatgatgga acgattagat acctataaac ttggtgaagt
    15181 taataaaaga catattaatg agcttaataa agtaataagt gaagagataa gagcacagct
    15241 aggcattaaa aataaaaagg aattgcaaac gaaaataaac aaatatttac tgattattta
    15301 aataataaga attggggacc ggtcgacaag aatattagtc atcatgggaa aaattatggt
    15361 tttcaattaa ctcctgcctc tcatatgaag atagggaata aaaatatatt tgtcaaagag
    15421 tataatggaa aaggaatttg ttgtgcttct acgagagaga gtgatcatat cgcgaatatg
    15481 tggctttcaa aagtggtgga tgatgagggg aaggaaattt tttcgggtat cagacatgga
    15541 gttatatcag cctatggatt gaaaaaaaac tcatctgaaa gagctgttgc tgctcgtaat
    15601 aaagctgagg agttagtaag cgcagcatta tatagcagac cagagttatt atcacaggct
    15661 ttgtctggta aaacagtaga tttaaagatt gtttcgactt ctcttctgac gccgaccagt
    15721 ttaaccgggg gagaggagag tatgttaaaa gatcaggtga acgcattaaa agggttaaac
    15781 tctaaaaggg ggaagccaac gaaattatta attcggaata gtgatggtct tctgaaagaa
    15841 gtaagcgtta atctaaaagt ggtgacattt aactttggtg taaatgaatt agcgctaaaa
    15901 atgggcttag gctggaggaa tgttgacaag cttaatgatg aatcaatatg ttctttgttg
    15961 ggggataatt tcctcaaaaa tggtgtgatt ggaggttggg ctgcagaagc aatagaaaaa
    16021 aatccaccat gtaagaatga tgtgatatat ctggctaacc agataaaaga gattgtaacc
    16081 aaaaagttac aaaaaaatga taatggagag ccgtataaat tgtcacaaag aatgactcta
    16141 ttggcttata ctataggtgc tgtaccttgc tggaattgta agagtgggaa ggacagaaca
    16201 ggtatgcagg atgctgaaat taaaagagaa ataataagaa aacatgaaac aggtcagttt
    16261 tctcaattaa atagtaaatt atcctcagaa gagaaaagat tattttctac tattctaatg
    16321 aatagtggta atatggaaat ccaagagatg aatactggtg tgcccggaaa taaagtcatg
    16381 aaaaaattgc cgctatcttc gttagagcta tcttattctg aaagaatagg ggacccaaaa
    16441 atatggaata tggtgaaagg gtattcgtca tttgtataag aggaatatat ggaagattta
    16501 gcagatgtta tttgccgagc cttgggtatc cctttaattg atatcgatga tcaagcaatc
    16561 atgcttgatg atgatgtgct aatatatatt gaaaaagaag gagattcaat aaatttatta
    16621 tgcccttttt gtgcattgcc tgaaaatata aatgatctta tatatgcgtt gagcctaaat
    16681 tactcagaaa agatatgctt agctactgat gatgaaggcg gaaatctaat tgcgcgatta
    16741 gatcttaccg ggataaatga attcgaggat gtatatgtta atacagaata ttatatttcg
    16801 cgtgtgcgtt ggctgaagga tgaatttgcg cgaagaatga aggggtatta atgtcccgtt
    16861 tcgtatttat tttgctgtgt tttatcccat atttagggag agctgattgt tgggataagg
    16921 ctggtgaaag gtacaatatt ccatcaagtt tgttaaaagc gattgcggaa aaagaatccg
    16981 gatttaataa atctgcagtc aatgttaata acaatggaag taaagattat ggtataatgc
    17041 aaataaatga ctttcattct aaaagactta gagaaatggg atattctgag gaaatgctaa
    17101 ttagtcatcc atgtctttct gtacattatg cagcaaagtt attaaatgag tttatgatga
    17161 tgtatggaag agggtgggag gctgttggag cctataatgc aggaacgtct ccgaaaaaga
    17221 aaaaagaaag attaaagtac gcagaggata tctatagacg ctatttgcgg atagcagcag
    17281 agagcaagca gaataatcga aggatataag aggatttatg tctgaggcaa agaactcaaa
    17341 tcttgcacca ttcaggttat tagtgaaact cacaaacggt gtcggggatg agtttccgtt
    17401 atattatggg aataacttaa ttgttctcgg acggacgata gaaacacttg agtttggaaa
    17461 cgacaatttc ccagaaaata ttattcctgt aacagatagt aaatcggatg gtataattta
    17521 tttgaccata agcaaagata atatctgtca atttagtgat gaaaaaggtg agcaaattga
    17581 tatcaattca caatttaatt catttgaata tgacgggatt tcttttcacc ttaaaaatat
    17641 gagggaggat aaatcgagag gccatatact aaacggaatg tataagaacc actcggtgtt
    17701 ttttttcttt gctgttattg ttgtgttaat tataattttt tccctctctt taaagaaaga
    17761 tgaagtaaaa gaaattgctg aaattataga tgataagaga tatggaatag tcaacactgg
    17821 acagtgcaat tatattcttg ccgaaacaca aaatgatgcg gtatgggcgt ctgtagcctt
    17881 aaataaaacc ggatttacca aatgcagata tattctggtg tcaaacaaag aaataaatag
    17941 aattcaacaa tatatcaatc aacgattccc atttatcaat ttgtatgtgt tgaatttagt
    18001 gagtgataaa gcggaattac tggtgttttt aagtaaagaa agaaatagtt caaaagatac
    18061 cgaacttgat aaattgaaaa atgctctgat tgttgaattt ccgtatatca aaaatattaa
    18121 attcaactat cttagcgatc ataatgcaag gggggatgct aaaggtattt tcacaaaggt
    18181 aaatgtccag tataaagaga tttgtgaaaa taataaagtt acatattctg tcagagaaga
    18241 actgacagat gaaaagcttg agctcattaa tagactgatt tctgaacata aaaatatata
    18301 tggagatcag tatattgagt tttctgtact tttgatagat gatgatttta aaggtaaatc
    18361 atatcttaac agcaaagaca gttatgtaat gttgaatgat aaacactggt tttttttaga
    18421 taaaaataag tgaggataaa atgagtgtta cagtaccgaa tgatgattgg acattgagtt
    18481 cattatctga aacttttgat gatggaactc aaacattaca aggtgaacta acattggcac
    18541 tagataaatt agctaaaaat ccttcgaatc cacagttgct ggctgaatac caaagtaaat
    18601 tatctgaata tacattatat aggaacgcgc aatccaatac agtgaaagtg attaaggatg
    18661 ttgatgctgc aattattcaa aacttcagat aatagggagc attcatgaat tacatttatc
    18721 cagtcaatca ggttgatatt atcaaagcca gtgattttca atctcaagag atatcaagtc
    18781 tggaagacgt cgtgtcggct aaatatagtg atattaagat ggatacagat attcaagtat
    18841 cacaaataat ggagatggta agcaatccag aatcattaaa cccagaatct ttggccaagt
    18901 tacagacgac gctctcaaat tattcaatag gagtatcatt agctggcacg ttagcaagaa
    18961 aaacagtttc ggctgttgaa actttattaa agtcttaatt tatatgatta ggtataaagg
    19021 ttttatttta ttcttgttgc tgatgttgat tggatgtgag caacgtgaag agttaatttc
    19081 taatttatct caaagacagg caaatgaaat aatatctgtg ctagaacgcc ataatattac
    19141 tgctagaaaa gttgatggag gtaaacaggg gatctcggta caagtcgaaa aggggacatt
    19201 tgcatcggca gttgatttga tgcgcatgta cgatttgcca aatccggaga gagttgatat
    19261 ctcacaaatg tttcctacag attcattagt gtcttctcca agagctgaaa aggcccgttt
    19321 atatagtgct attgagcaac ggctggaaca gtctttagtt tctattggtg gtgttatttc
    19381 ggcaaaaata catgttagct atgatcttga agaaaaaaat atatcttcaa aaccgatgca
    19441 tatatcagta atcgctatat atgactcacc gaaagagtct gaactattag ttagtaatat
    19501 taagcgattt ttgaaaaaca ccttttctga tgttaagtat gaaaatatat ctgtcatatt
    19561 aactccgaaa gaagaatatg tttatacaaa tgtacaacct gttaaggaag ttaaatcgga
    19621 atttttaaca aatgaagtaa tatatttatt tctcgggatg gctgtactag ttgtcattct
    19681 tttggtatgg gcattcaaaa cagggtggtt caagagaaac aaaatatgat aagaatggat
    19741 ggaatttata aaaaatatct ttcaataatt tttgatccag cgttctatat aaatagaaat
    19801 cggttgaatt tgccttctga actgttagaa aatggcgtaa tcagaagtga gattaataat
    19861 ctcataatta ataaatatga tctaaattgc gatattgaac ctttaagcgg ggtaaccgct
    19921 atgtttgttg ccaactggaa tttacttcca gctgttgctt attttatcgg tagtcaagag
    19981 tctcggttaa ttaatcatag tgagatggtt attagttatt atgggggcaa aatcagtaag
    20041 caaggggagg ccgctattcg aagtggtttt tggcatctta ttgcgtggaa ggaaaatata
    20101 tctgttggta tatatgaaag aataaactta ttatttaatc caattgcttt ggagggaaat
    20161 tatacgcctg tagaaagaaa tttgtcccga ttaaatgaag gtatgcaata tgcaaaaagg
    20221 cactttaccg gtatccagac atcatgccta tgatggtgtt gttataaaga gaattgaaaa
    20281 ggaattatgt aaaaccataa aagatagaga cactgagagt aagaagaaag caatttgtgt
    20341 tataaaggag gctacgaaaa aagctgaatc actgcgtata gatgctgtat gtgatggtta
    20401 tcaaattggt atacagactg cttttgagca tattattgac tatatttgtg aatggaagtt
    20461 aaagcaaaat gaaaatagaa gaaatattga ggactatata actagtttgc tttcagaaaa
    20521 tttacatgat gagaggatca ttagcacttt attggagcag tggcttagtt cgttgcgtaa
    20581 tacagtaaca gaattaaaag tggttttgcc caagtgtaat ttagcactga ggaaaaagtt
    20641 agaacttgat ctccataaat atcgctcaga tgtcaagatt attttaaaat attctgaagg
    20701 gaataattat atattctgta gtggtaatca agtagtggag tttagtcctc aggatgtaat
    20761 atcaggtgtt aaaatagaac ttgcggaaaa attaacaaaa aatgataaaa aatatttcaa
    20821 agagttagca cataagaaac tgagacaaat tgcagaagac ttgttaaaag agaatcctgt
    20881 taatgattaa tcaaataaat gcaagcaatg ctctgcagca aagattaaat agtgaagaag
    20941 ttgttaattt aaatgagaga ttatcatctt cgcaatcatt tgatgaggat ataatctatg
    21001 aaataatgca atatttctca cagagtgaat taaattccat agataatgat gaactgcaca
    21061 ataaaattga acaattattc aattcacgtt ttccttatct tacagctgca caaaaaagtt
    21121 cacttcttaa taaactcata gatgcaaatc aatatgtcga tttgcatgag ggattttatg
    21181 catcattgtc catatataac aatattgatt tctatattaa gactacaacc ttcgattcat
    21241 taattagtgt ctttgaagca gggagagagg cagatgattc gacatggtag taataagttg
    21301 aaaatattta ttttaagtat attgctatta acactgagtg ggtgtgcttt aaagtcatca
    21361 tctaattctg aaaaagaatg gcatattgtt cctgtaagta aggattattt ttctattcca
    21421 aatgatttat tatggtcgtt taatacaacc aataaaagta taaatgttta ctctaaatgt
    21481 attagtggta aggcggttta tagttttaat gcaggtaaat tcatgggcaa ctttaatgtt
    21541 aaggaagtag atgggtgctt catggatgca caaaagatag ctatagataa actattttct
    21601 atgctgaaag acggggttgt tttaaaaggt aataagataa atgataccat ccttatagag
    21661 aaggatgggg aagttaaatt aaaattaatt cgagggatat aattgtattg tgagtaaata
    21721 taaaggtcta aatacaagta atatgtttta catttactct agtggacatg aaccagttaa
    21781 cgttgagctt gtaaaagata aagaacgtaa cataattgag ctggctccag catggaaggg
    21841 tttttttttg tccgaaatca aaatataaaa ttcagtgata atgtgaacta tcattatcgc
    21901 tttaacatta atagttgtgc aaaatttctt gctttttggg actatttcag tggtgctcta
    21961 gtagagcata gccatgctga aaaatgtatt catttctatc atgagaatga tcttcgggat
    22021 agttgtaata ccgaatcgat gcttgataag ttaatgttaa gattcatctt ttcatctgac
    22081 cagaatgtaa gtaatgcact ggctatgata cgaatgactg agtcatatca tttggttctt
    22141 tatcttctga gaactattga gaaagaaaag gaggtaagaa taaagagtct gacagagcat
    22201 tatggtgtgt cggaggctta ttttagatct ttatgtagaa aagctttagg ggcaaaagtc
    22261 aaagaacaac tgaacacttg gcggttagta aatggtttat tagatgtatt tttgcacaat
    22321 caaacaatca cttcagctgc catgaataat gggtatgcat caacatccca tttttcaaat
    22381 gagataaaaa cacgactagg cttttctgca agagaattat cgaatattac ttttttagtg
    22441 aaaaaaataa atgaaaaaat ttaatattaa atctttgact ctcttgattg tattgttacc
    22501 attgatagtt aatgctaata atattgactc tcatttatta gaacaaaatg acatagcgaa
    22561 atatgttgct caaagtgata cggtaggatc tttttttgaa cgtttttctg cgttattaaa
    22621 ttatcctatt gttgttagca aacaagctgc aaaaaagaga atatctgggg agtttgattt
    22681 atctaatcca gaagaaatgc ttgaaaaact gacattactt gtgggactca tatggtataa
    22741 agatggaaat gcattataca tatatgactc tggagaatta ataagcaaag ttattttgct
    22801 ggagaatata tcacttaatt atcttattca gtatctgaag gatgctaacc tttatgatca
    22861 cagatatcct ataagaggaa acattagtga taaaacattc tatatatctg gtcctccggc
    22921 attggttgaa ctggtagcta atacagcaac tttgcttgat aagcaggttt cttctattgg
    22981 gacagataaa gttaattttg gggttataaa attaaaaaat acatttgtta gtgacagaac
    23041 ttataatatg agaggggagg atattgtcat cccaggggtg gctactgttg tagaacgttt
    23101 attaaataat ggtaaggctc tgagtaacag acaagcccaa aatgatccga tgccaccctt
    23161 taatattaca cagaaagtat ctgaggatag taatgacttt tcatttagtt cagttaccaa
    23221 tagttctata ttagaggatg tatcacttat tgcatatcca gaaaccaatt ctatattagt
    23281 caaaggtaat gatcagcaaa tacagattat tcgcgatatt atcactcagt tagatatagc
    23341 caaacgacat atagaattat cattatggat aatcgatatt gataaatctg aattaaataa
    23401 ccttggtgtt aattggcaag gaacggcttc atttggtgat tcgtttggtg cgtcatttaa
    23461 tatgagctca tcagcatcga taagcacttt ggacggcaat aagtttattg cttcagttat
    23521 ggcattaaat cagaaaaaga aagcaaatgt agtttcaagg ccggtgattt tgacacaaga
    23581 gaatattccg gctatatttg ataataacag gactttttat gtgtcattag ttggtgagcg
    23641 caattcttcg ttggagcatg ttacgtatgg aacattgata aatgttatac ctcgcttttc
    23701 atctcgggga caaattgaga tgtctttaac gatagaggat ggaacaggaa atagtcagtc
    23761 aaattacaat tataataatg aaaatacatc tgtccttcca gaagttggaa ggaccaaaat
    23821 cagtacaata gcgagagttc cacaaggcaa aagtttgttg ataggtggtt atacgcatga
    23881 aacgaactct aacgaaataa tatctattcc ttttctctcc tctattcctg taatcggtaa
    23941 tgtgttcaag tataaaacaa gtaatatcag taatattgta agggtttttc tgattcaacc
    24001 ccgtgagata aaggagtcaa gttattataa tactgcagaa tataaaagcc tcatatctga
    24061 aagagaaata caaaagacta cacaaataat cccatctgaa actactttgc tggaagacga
    24121 aaaatcattg gtttcatact taaattacta actataaagt aggtgatgta tgcttgatgt
    24181 taaaaataca ggagttttta gctctgcatt cattgataag ttgaatgcaa tgacaaattc
    24241 agatgatgga gatgagactg ctgatgcaga gcttgattct ggcttggcta atagcaagta
    24301 tattgactca tctgatgaga tggcttccgc tctttcgtca tttataaaca gaagagacct
    24361 tgagaaactg aaaggaacaa atagtgatag tcaggaacgt attttagatg gggaagaaga
    24421 tgaaattaat cacaagattt ttgatttaaa gagaacgtta aaagataacc ttcctctaga
    24481 tcgggatttt atagacagac taaagagata ttttaaagat ccaagtgatc aagtcttagc
    24541 attaagggaa cttttgaatg aaaaagatct tactgctgaa caagtcgaat tattaactaa
    24601 aattattaat gagataatat caggtagtga aaaaagtgtt aatgctggaa taaattcagc
    24661 tatacaggct aaattatttg gcaacaaaat gaaacttgaa ccacagcttt tgcgtgcatg
    24721 ttatcgtggt tttatcatgg ggaacatatc aacaacagat cagtatatag aatggcttgg
    24781 taattttggt tttaatcaca gacatacaat tgtgaatttt gtagagcagt cactgattgt
    24841 agacatggat tctgagaaac cgagctgtaa tgcttatgag tttggttttg tgttatctaa
    24901 attaattgca attaagatga ttagaacttc agacgtaatt tttatgaaga aactggaatc
    24961 ctcaagcttg ctaaaagatg gcagtttaag tgcagagcag ctattgctaa ctttattata
    25021 tatttttcaa tatccaagtg aaagtgagca aattcttact tctgttatag aagtatcacg
    25081 agccagtcat gaggattctg tagtgtatca aacatatcta tcttctgtta atgaaagtcc
    25141 tcatgatata tttaaaagtg aaagtgaaag agaaattgcg atcaatattc tacgagagct
    25201 tgtcacaagt gcatacaaga aagagctttc tagataacag gagataaaag tgatccagtc
    25261 ttttcttaag caagtaagta ctaagcctga attaattata ttagtgctta tggttatgat
    25321 cattgctatg ttgatcatac cattacctac ataccttgtt gattttttaa tagggcttaa
    25381 tattgtactt gctattctcg tttttatggg atcattttat attgaaagaa tattgagttt
    25441 ttctacattc ccttcggttt tgttgataac gacattattt cgtctcgctt tatcaataag
    25501 tactagtcga ctaattttag ttgatgcgga tgcgggtaaa attattacta catttggcca
    25561 gtttgtcatt ggtgatagtc tggctgtagg ttttgttatt ttttcaatag taactgttgt
    25621 gcagttcatt gttataacaa aaggatctga aagggttgcg gaggtagctg ctcgcttttc
    25681 tcttgacggt atgccgggga aacaaatgag cattgatgcg gatttaaagg ctggaattat
    25741 tgatgccgca ggagctaaag aaagacgcag tattctggaa cgtgagagtc aattgtatgg
    25801 ttcatttgat ggagcaatga agttcattaa aggtgatgca attgctggca tcattattat
    25861 ttttgtcaat ttaataggag gtatttctgt tggtatgagt caacatggaa tgtccctatc
    25921 tggtgcatta tctacttata ccatactaac tattggtgac ggattggtct ctcagattcc
    25981 tgctctgtta atttctataa gtgcaggatt tatcgttaca agagttaatg gtgatagcga
    26041 taatatggga cgtaacataa tgtcccagat atttgggaat ccttttgttc tcattgttac
    26101 atcagctctt gccttggcga tagggatgtt gccaggtttt ccgttttttg tttttttcct
    26161 gatagcagtt actttgacgg ctttattcta ttataaaaag gtcgtagaaa aagaaaaaag
    26221 tctgtctgag tctgattcta gtggctatac tggtacattt gatattgata atagccatga
    26281 ctcatctttg gcaatgatag aaaatctgga tgctattagt tcagaaaccg ttcctttaat
    26341 tttattattt gccgaaaata agataaatgc aaatgatatg gaaggtctta ttgaaaggat
    26401 aagaagtcag ttctttattg attatggtgt caggcttcca actattttat atagaacaag
    26461 caatgagctt aaggtcgatg atattgtttt gttaataaat gaggtgcgtg ccgatagttt
    26521 taatatatat tttgacaaag tgtgtattac agatgaaaat ggagatatag atgctctagg
    26581 tattcctgtt gtttcaactt catataatga acgtgttatt tcttgggtag acgtttcata
    26641 tacagaaaat ctaactaata ttgatgctaa aattaaaagt gctcaagatg aattttatca
    26701 ccagttgtca caagctttat taaacaacat aaatgagatt tttggtatac aagaaacaaa
    26761 aaatatgtta gatcagtttg aaaatcggta tcctgatcta ttaaaggaag tcttccgaca
    26821 tgtgactata cagagaattt ctgaggtatt acaaagattg cttggagaaa atatttctgt
    26881 tcgcaattta aaacttatta tggagtcttt ggcgctttgg gctcccagag aaaaagatgt
    26941 cataacatta gttgaacatg tccgtgcatc actttctagg tatatttgta gtaaaatagc
    27001 tgtttctggt gagattaaag ttgtgatgct ttccggatat attgaggatg caataagaaa
    27061 agggataagg caaacctctg gtggctcttt cttgaatatg gatatagagg tttcggatga
    27121 ggtaatggaa actttagcac atgctttgag agaattgaga aatgcaaaaa aaaatttcgt
    27181 tcttttggta tcagtagata tacgtaggtt tgttaaaaga cttatagata acagatttaa
    27241 gagtatactc gttatatcgt atgctgagat tgatgaagca tataccatta atgtattaaa
    27301 gactatttag tgaggtttaa atatgagtaa cattaattta gttcaattag ttagagatag
    27361 tcttttcacg attggttgtc ctccatcaat tattacagat ttagacagcc attcagcgat
    27421 taccatttct ttggatagta tgcctgctat taatatagct cttgtcaatg agcaggttat
    27481 gttatgggct aattttgatg caccaagtga tgtaaaactt cagtcatctg cttataacat
    27541 attaaattta atgttaatga actttagtta tagcattaat gagctggtag agcttcatcg
    27601 ttctgatgag tacttacagt taagagttgt tattaaagat gactacgttc acgatgggat
    27661 tgtttttgca gagattctgc atgagtttta tcagaggatg gaaatcttaa atggggtctt
    27721 ataatcaatg agctatacaa aattgctcac tcaattatct tttcctaata gaatctcggg
    27781 gccaatcttg gaaacaagtc ttagcgatgt ttcgattggt gagatttgta acattcaggc
    27841 tggaattgaa agtaatgaaa ttgttgcaag agctcaggtt gtaggatttc atgatgaaaa
    27901 aacaatatta agcttgattg gaaattctcg tggactttca cggcaaacgt tgattaagcc
    27961 cactgcccag tttcttcata cgcaagtcgg ccgtggatta ttgggagcag tagtcaatcc
    28021 tttaggggag gttactgata agtttgctgt tacagataac agtgaaattc tttatcgacc
    28081 tgtagataat gctcctccgc tatatagtga aagggctgca attgagaagc cttttttaac
    28141 aggtattaag gttattgatt ctttactcac gtgtggtgaa ggacagcgaa tggggatttt
    28201 tgcgtcagct ggttgtggca aaacttttct catgaatatg ctcattgaac atagtggtgc
    28261 tgatatatat gttattgggt taattggtga gcgaggtcga gaggttactg aaacggttga
    28321 ttatttgaaa aactctgaga aaaaaagcag gtgtgtttta gtatatgcaa cttcggatta
    28381 ctcttcggtt gatcgttgta atgctgcata tatagccact gctatagccg aattttttag
    28441 gactgaagga cataaagtag cgctttttat tgattcatta acaaggtatg ccagagcatt
    28501 acgtgatgtg gccttagccg ctggagaatc acctgccaga agaggctatc cggtttcggt
    28561 ttttgatagc ttacccagac ttcttgaaag gccaggaaag ttaaaggcag gtggctctat
    28621 tactgcattt tacactgttc ttttggagga tgatgatttt gctgatccat tagctgaaga
    28681 ggtaagatcc attttagatg gacatatata tttgagcaga aatctagccc aaaaaggaca
    28741 atttcctgca attgattcct taaaaagtat aagcagggta tttacacagg ttgttgatga
    28801 aaaacatcgt attatggccg ctgcatttcg ggagctactt tctgaaatag aagagctaag
    28861 gacaattatt gactttggtg aatacaaacc gggggagaat gcctctcagg ataaaatcta
    28921 caacaaaata tctgttgttg agagttttct gaagcaagat tatcgactcg gttttactta
    28981 tgagcagaca atggagctta ttggtgaaac aattagataa ggttttaaaa atcaaagata
    29041 aatatcaacg gtctgttaaa ttaattgaag cacacatatt aacactaaaa aaaaaactct
    29101 ttgtatcgag atgtggaggc attagataaa aggattatat acttcctgca actggagaat
    29161 gacttagaac ctgtaggcgc tcagagtgtt agtcagcttt tcaatacaag acgtaagatc
    29221 gctatagtaa aaaagcatat tatccaatat cagtctgaac gaattcttct aaagggaaga
    29281 attgaagaaa ttcaaaaaga tattgatgag gcaaatgctt ctaaaagaaa actgcttcat
    29341 aaagagagta aaatatgtaa acggattggc ttgattaaaa gaaataactt tgccaaacag
    29401 ttgatacttg atgagttatc acaagaggat atgaagtatg gcattagata atataaacct
    29461 aaattttagt agtgacaaac agatagaaaa atgtgagaaa ctatcttcaa tagataatat
    29521 tgatagtctc gttttgaaga agaagaggaa ggttgaaatt cctgagtact ctttaattgc
    29581 atctaactat tttactattg ataagcactt tgaacataag catgataaag gagaaattta
    29641 tagtggcatt aaaaatgcgt tcgaacttag aaacgaacga gcgacatatt ctgatattcc
    29701 agaatcaatg gccattaaag aaaatatttt gataccagat caagatatca aagcaaggga
    29761 aaaaataaat atcggcgata tgagggggat cttttcatat aataagagtg gaaatgcaga
    29821 caagaacttc gaaagaagtc atacttcttc tgtaaaccct gataatctgc tagaatctga
    29881 taatagaaat ggtcaaattg gtttaaaaaa tcatagcttg tctattgata agaatattgc
    29941 tgacatcatt tctttactaa atggaagtgt tgctaaatca tttgagctgc ctgtaatgaa
    30001 taaaaatact gcagacataa ccccatccat gtcattgcaa gaaaaatcaa tagttgaaaa
    30061 tgataaaaat gtttttcaaa aaaatagtga aatgacttac cactttaaac agtggggggc
    30121 tggacattct gttagtattt cagtggagtc tggttctttt gttctaaaac cgtcagatca
    30181 atttgtagga aataaacttg acttaatttt gaaacaagat gctgagggta attacagatt
    30241 tgatagcagt caacataata aggggaataa aaataatagt acaggatata atgaacagag
    30301 tgaagaagaa tgctaagaat taaacatttt gacgctaacg aaaaactaca gattttatat
    30361 gcaaagcaac tctgcgagcg tttttcgatt cagacattca aaaataaatt tacaggcagt
    30421 gaaagtttag tcactcttac ttccgtgtgt ggggattggg taattcgtat tgatacatta
    30481 tcttttttga aaaaaaaata cgaggtattt tcaggatttt ctacacaaga atctttactg
    30541 catttatcaa aatgtgtctt tatagagtcg tcatctgtat tttcgattcc agaactgtct
    30601 gataagatta ctttccggat cacgaatgaa atccagtatg caactactgg gagtcattta
    30661 tgctgttttt cctcttcttt aggtattatt tattttgaca agatgccggt attacgtaat
    30721 caagtttctc ttgactcatt gcatcatctt ttagagtttt gcttaggttc atctaatgta
    30781 aggctggcta ctttaaaaag aattcgcact ggtgatataa tcatagttca gaaactttat
    30841 aatttattat tgtgtaatca agttattatt ggggattata ttgtgaatga taataatgag
    30901 gcaaaaatta atctgtcaga aagtaatggt gagtcagaac acacagaagt ttctttggca
    30961 ttattcaatt atgatgatat caatgtaaaa gtggacttta ttcttttaga aaaaaatatg
    31021 acaatcaatg aactaaaaat gtatgtagaa aacgaattat tcaagtttcc cgatgacata
    31081 gttaaacatg taaatattaa agtaaatggt tctttggttg ggcatgggga acttgtttct
    31141 attgaggatg gttatggtat cgagattagt tcttggatgg taaaggagta atctcatgct
    31201 gagtgacatg tccctcatcg ctacactttc tttttttacg ctattaccat ttttagttgc
    31261 tgctggtacc tgttatataa agttttctat tgtttttgta atggtacgaa atgcacttgg
    31321 tttgcagcag gttccatcaa atatgactct caatggtatt gcattgatta tggctttgtt
    31381 cgtgatgaaa cccattattg aagctggtta tgaaaattat ctaaatggcc ctcaaaaatt
    31441 tgatacaata tctgatattg tccgtttttc tgattctggc ttgatggaat ataagcaata
    31501 cttgaaaaag catacagatc ttgagttagc acgttttttt caaaggtctg aagaagaaaa
    31561 tgctgattta aagagtgcag agaataatga ttattcattg ttttccctct tgcctgcata
    31621 tgcgctaagt gaaatcaaag atgcgttcaa aataggtttt tatttatatt tgccatttgt
    31681 tgttgttgat cttgttattt caagcatttt acttgcactt ggtatgatga tgatgagtcc
    31741 aatcacaata tcagtaccaa taaaacttgt attatttgtt gcgcttgatg ggtggggtat
    31801 cctgtctaag gccttgattg aacaatatat caatattcct gcttagatga tggaggtaat
    31861 gtgtctgata tagtttatat gggtaataag gctctttatt taatccttat cttttcctta
    31921 tggccagtag gtatagctac ggttattgga ttatctattg gtttgttaca gacagtgact
    31981 caactccaag agcagacact tccttttggt ataaagctta taggtgtctc aatatctttg
    32041 ctacttcttt ctggatggta tggtgaggtt ttattgtctt tttgtcatga aataatgttt
    32101 ttaattaaga gtggggtttg atggacattt caagctggtt cgaaagtatt catgtgtttt
    32161 taatactcct gaacggcgtt ttttttagat tggctccatt atttttcttt cttccatttt
    32221 taaataacgg tataatttct ccatctatta gaatacctgt gatttttctt gttgcatcag
    32281 gattaattac ttctggtaag gtagacatag gttcttctgt ttttgaacat gtttatttcc
    32341 ttatgttcaa ggaaataatt gttggcctcc ttctctcttt ttgcttgtct cttccctttt
    32401 ggatatttca tgctgttggt agcattattg acaaccagcg tggggcaacg cttagtagtt
    32461 caattgatcc tgccaatggt gttgatacgt ctgagttggc aaaatttttc aatctttttt
    32521 ctgcagttgt atttctatac agtggtggta tggtctttat tttagaatcc atacaattgt
    32581 cttataatat atgcccgtta ttttctcaat gttctttccg tgtctcaaat atcttaacat
    32641 ttctgacttt attggcaagt caggctgtta ttttagccag tcctgttatg atagtattgt
    32701 tactatcaga agtattactt ggtgtattat cgagatttgc tccgcagatg aatgcttttt
    32761 ccgtatcatt aactattaaa agtttacttg caatatttat tattttcatc tgttcttcta
    32821 ctatttactt ttctaaagtt caatttttcc tcggtgaaca taaatttttc acaaatctat
    32881 ttgttagata aaatattatg gcaaataaaa cagaaaagcc gacacctaaa aaactaaagg
    32941 atgccgcaaa aaaaggacag tcatttaaat ttaaggattt aacgactgtt gttattattc
    33001 tggtagggac atttactata atatcattct tttccttaag tgatgtaatg ctcttataca
    33061 gatatgtaat aattaatgac ttcgaaatta atgagggtaa atactttttt gcagtggtta
    33121 ttgtcttttt taagataatt ggcttcccac tttttttctg tgttctttcg gctgtgttgc
    33181 caacattggt tcaaacaaag tttgttcttg cgactaaagc tatcaagatt gatttttcag
    33241 tattaaaccc tgttaaaggg ttaaaaaaaa tatttagtat aaagacaata aaagaatttt
    33301 tcaaaagcat tctgcttctt attattctag cattaacaac ctatttcttt tggattaatg
    33361 accgaaaaat aattttttct caggtgtttt ctagtgttga tggcttatat cttatttgg
//
LOCUS       DAZXUL010000040        32150 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZXUL010000040 DAZXUL010000000
VERSION     DAZXUL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32150
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.040"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..172)
                     /locus_tag="ACH3L1_001838"
     CDS             complement(<1..172)
                     /locus_tag="ACH3L1_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076591.1"
                     /translation="MLRMFIPTSNGKISRHRYIFLFILINFIFAFLIIFFNDGEAGFL
                     VIVSTIALHYLVI"
     gene            complement(267..1157)
                     /gene="rarD"
                     /locus_tag="ACH3L1_001839"
     CDS             complement(267..1157)
                     /gene="rarD"
                     /locus_tag="ACH3L1_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFT4076592.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1209..1676)
                     /gene="yigI"
                     /locus_tag="ACH3L1_001840"
     CDS             complement(1209..1676)
                     /gene="yigI"
                     /locus_tag="ACH3L1_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFT4076593.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1841..2710
                     /gene="pldA"
                     /locus_tag="ACH3L1_001841"
     CDS             1841..2710
                     /gene="pldA"
                     /locus_tag="ACH3L1_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFT4076594.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2843..4672
                     /gene="recQ"
                     /locus_tag="ACH3L1_001842"
     CDS             2843..4672
                     /gene="recQ"
                     /locus_tag="ACH3L1_001842"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFT4076595.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4736..5356
                     /gene="rhtC"
                     /locus_tag="ACH3L1_001843"
     CDS             4736..5356
                     /gene="rhtC"
                     /locus_tag="ACH3L1_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFT4076596.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5418..6038)
                     /gene="rhtB"
                     /locus_tag="ACH3L1_001844"
     CDS             complement(5418..6038)
                     /gene="rhtB"
                     /locus_tag="ACH3L1_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFT4076597.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6149..7171
                     /gene="pldB"
                     /locus_tag="ACH3L1_001845"
     CDS             6149..7171
                     /gene="pldB"
                     /locus_tag="ACH3L1_001845"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFT4076598.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7179..7979
                     /gene="yigL"
                     /locus_tag="ACH3L1_001846"
     CDS             7179..7979
                     /gene="yigL"
                     /locus_tag="ACH3L1_001846"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFT4076599.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8055..8954
                     /gene="bioP"
                     /locus_tag="ACH3L1_001847"
     CDS             8055..8954
                     /gene="bioP"
                     /locus_tag="ACH3L1_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFT4076600.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8842..9795)
                     /gene="metR"
                     /locus_tag="ACH3L1_001848"
     CDS             complement(8842..9795)
                     /gene="metR"
                     /locus_tag="ACH3L1_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFT4076601.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10031..12292
                     /gene="metE"
                     /locus_tag="ACH3L1_001849"
     CDS             10031..12292
                     /gene="metE"
                     /locus_tag="ACH3L1_001849"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFT4076602.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12331..13146)
                     /locus_tag="ACH3L1_001850"
     CDS             complement(12331..13146)
                     /locus_tag="ACH3L1_001850"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFT4076603.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13408..14169
                     /gene="udp"
                     /locus_tag="ACH3L1_001851"
     CDS             13408..14169
                     /gene="udp"
                     /locus_tag="ACH3L1_001851"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFT4076604.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14310..15737
                     /gene="rmuC"
                     /locus_tag="ACH3L1_001852"
     CDS             14310..15737
                     /gene="rmuC"
                     /locus_tag="ACH3L1_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFT4076605.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15832..16587
                     /gene="ubiE"
                     /locus_tag="ACH3L1_001853"
     CDS             15832..16587
                     /gene="ubiE"
                     /locus_tag="ACH3L1_001853"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFT4076606.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16601..17206
                     /gene="ubiJ"
                     /locus_tag="ACH3L1_001854"
     CDS             16601..17206
                     /gene="ubiJ"
                     /locus_tag="ACH3L1_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFT4076607.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17203..18843
                     /gene="ubiB"
                     /locus_tag="ACH3L1_001855"
     CDS             17203..18843
                     /gene="ubiB"
                     /locus_tag="ACH3L1_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFT4076608.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18922..19191
                     /gene="tatA"
                     /locus_tag="ACH3L1_001856"
     CDS             18922..19191
                     /gene="tatA"
                     /locus_tag="ACH3L1_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFT4076609.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19195..19710
                     /gene="tatB"
                     /locus_tag="ACH3L1_001857"
     CDS             19195..19710
                     /gene="tatB"
                     /locus_tag="ACH3L1_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFT4076610.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19713..20489
                     /gene="tatC"
                     /locus_tag="ACH3L1_001858"
     CDS             19713..20489
                     /gene="tatC"
                     /locus_tag="ACH3L1_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFT4076611.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20531..21313
                     /gene="tatD"
                     /locus_tag="ACH3L1_001859"
     CDS             20531..21313
                     /gene="tatD"
                     /locus_tag="ACH3L1_001859"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFT4076612.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21310..21798)
                     /gene="rfaH"
                     /locus_tag="ACH3L1_001860"
     CDS             complement(21310..21798)
                     /gene="rfaH"
                     /locus_tag="ACH3L1_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFT4076613.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21965..23458
                     /gene="ubiD"
                     /locus_tag="ACH3L1_001861"
     CDS             21965..23458
                     /gene="ubiD"
                     /locus_tag="ACH3L1_001861"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFT4076614.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23504..24205
                     /gene="fre"
                     /locus_tag="ACH3L1_001862"
     CDS             23504..24205
                     /gene="fre"
                     /locus_tag="ACH3L1_001862"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFT4076615.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24397..25560)
                     /gene="fadA"
                     /locus_tag="ACH3L1_001863"
     CDS             complement(24397..25560)
                     /gene="fadA"
                     /locus_tag="ACH3L1_001863"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFT4076616.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25570..27759)
                     /gene="fadB"
                     /locus_tag="ACH3L1_001864"
     CDS             complement(25570..27759)
                     /gene="fadB"
                     /locus_tag="ACH3L1_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFT4076617.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27949..29280
                     /gene="pepQ"
                     /locus_tag="ACH3L1_001865"
     CDS             27949..29280
                     /gene="pepQ"
                     /locus_tag="ACH3L1_001865"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFT4076618.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29280..29894
                     /locus_tag="ACH3L1_001866"
     CDS             29280..29894
                     /locus_tag="ACH3L1_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFT4076619.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29933..31384
                     /gene="trkH"
                     /locus_tag="ACH3L1_001867"
     CDS             29933..31384
                     /gene="trkH"
                     /locus_tag="ACH3L1_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFT4076620.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31396..31941
                     /gene="hemG"
                     /locus_tag="ACH3L1_001868"
     CDS             31396..31941
                     /gene="hemG"
                     /locus_tag="ACH3L1_001868"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFT4076621.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7503 a         8160 c         8773 g         7714 t
ORIGIN      
        1 tgatgacgag ataatggagt gcgatggtag agactataac aagaaaacct gcttccccat
       61 cgttaaaaaa gataataaga aaggcaaaaa tgaaatttat taaaataaat aaaaatatat
      121 atcggtgtct ggaaatctta ccgttagatg ttgggataaa catacgtaac ataaatttta
      181 catccttgtg tgagtctcct gtcagcatgg caatatgccc actctcatac aagagtcggc
      241 atattttttc agaatatatt tattttttat ttggacgttc tacgctgcgt ataaatcgcg
      301 tccatcacaa aaattgccag cgccacccaa ataaaggtga aagtcaccat cttatcggca
      361 cccggttttt caccataaaa cgtcacagcc agcaggaaca tcagcgtcgg gccaatgtac
      421 tgaaaaaagc ctaacgttga gagacgcagg cgcgtggcag cggcagtaaa acacaacagc
      481 ggtacggtag tgacaatacc ggcggcgatc agcagtaaat tcagcgacat cgggttttgc
      541 cccatatggc tggttgaact gtcggcaata gcaaacaggt aaatcgccgc cacgggcagc
      601 agccacatgg tttcaattaa catgccggtt tgtgcttcta ccgcaatctt cttgcgcacc
      661 aggccgtaga aggcaaaact aaatgccagc cccagcgcga taataggtag cgaaccaaaa
      721 gtccacagct ggaccaacac gccacatatc gccagaatca ccgccagcca ttgcatccgg
      781 cggaatcgct cgccgaggaa aatcatcccc agcacaatgt tcaccagcgg gttaataaag
      841 taaccaaggc tcgcttccag catatggtga ttgttcaccg cccagataaa cagtagccag
      901 ttgccaccaa tcagtacggc agagactgcc agcataaaaa ttttctgtgg cgtctgaatc
      961 agcgttttta aataggacca ctggcggcaa atgctcatca gcaccaccat aaagaaaaac
     1021 gaccagatca cgcgatgcgt caggatctca tcggcgggca cgtagtaaat cagcttgaag
     1081 tatgctggcg caatacccca aataaaataa gcggcaagag cgagtaatac gccctgccgc
     1141 gtttgttttg catccatcgg gaatactcat ttttaattgg taacagcagt ttacctgctt
     1201 ttatgtcttc aacctaccat ataggtggcg gtggcactgg caatataaag ctgttcttca
     1261 ttgtgtaatt caacgcgggc gacggcgact ttattgcctg cacgcaacag gctactggta
     1321 gcagtaaaac gctcgcccct gcctgggcgc agataatcaa cgcgaagatc aatggtcccc
     1381 atccgcgata gccgctggcg tagttcatct tcactgatgg tttcgtggcg ggttaaggta
     1441 cttcccacgc acaccagacc ggcggcgaca tccagcgccg acgcaatgac tccgccgtgc
     1501 aaaatgcttt gcgcccagtt acccaccatc atcggctgat ttttaaaggc cagctgtgcg
     1561 aactcttttt cgtaacgctc cagttccatc cccaatgcgc ggttaaatgg catgtgataa
     1621 acaaacatct cacccactaa tttcagggct tgttcagcgg tcagtacggc agacatatca
     1681 tccttacact tcattggtta atgaaatgtt gattttatgc ttctttgttg ttggtttcta
     1741 ctttaggaag ggataactaa cggcaatgga gttaagtatg taaaatagcc cgcagaaaaa
     1801 cattcacctt atcaataatt cgttacggag aacacgaccg atgcggactc tgcagggctg
     1861 gttgttgccg gtgtttatgt tgcctatggc agtatatgca caagaggcaa cggtgaaaga
     1921 ggtgcatgac gcgccagcgg tgcgtggcag tattatcgcc aatatgctgc aggagcatga
     1981 caatccgttc acgctctatc cttatgacac caactacctc atctacaccc aaaccagcga
     2041 tctgaataaa gaagcgattg ccagttacga ctgggcggaa aatgcgcgta aggatgaagt
     2101 aaagtttcag ttgagcctgg catttccgct gtggcgtggg attttaggcc caaactcggt
     2161 gttgggtgcg tcttatacgc aaaaatcctg gtggcaactg tccaatagcg aagagtcttc
     2221 accgtttcgt gaaaccaact acgaaccgca attgttcctc ggttttgcca ccgattaccg
     2281 ttttgcaggt tggacgctgc gcgatgtgga gatggggtat aaccacgact ctaacgggcg
     2341 ttcagacccg acctcccgca gctggaaccg cctttatact cgcctgatgg cagaaaacgg
     2401 taactggctg gtagaagtga agccgtggta tgtggtgggt aatactgacg ataacccgga
     2461 tatcaccaaa tatatgggtt actaccagct taaaatcggc tatcacctcg gtgatgcggt
     2521 gctcagtgcg aaaggacagt acaactggaa caccggctac ggcggcgcgg agttaggctt
     2581 aagttacccg atcaccaaac atgtgcgcct ttatactcag gtttacagcg gctatggcga
     2641 atcgctcatc gactataact tcaaccagac ccgtgtcggt gtaggggtta tgctaaacga
     2701 cttgttttga tgaacggttg agtggttggc aaatctggaa tccagcatcc aggattaccc
     2761 tctcagagac taaaagcatt gcagtttctc gcgcaggcgc tgaaaatagc gcctgttttt
     2821 atttcaggca atcggggtga atgtggcgca ggcggaagtg ttgaatctgg agtccggagc
     2881 taaacaggtt ttacaagaaa cctttggcta ccaacagttt cgccccggcc aggaagaaat
     2941 tatcgacact gtgctttccg gtcgcgattg cctggttgtt atgcccactg gtggcggaaa
     3001 atccctttgc tatcaaattc ctgccttatt gctaaacggc cttaccgtgg ttgtttcacc
     3061 gctgatttcg ttgatgaaag atcaggtgga tcaactacag gccaacggcg tggcggcggc
     3121 gtgccttaac tcgacgcaaa cccgcgaaca gcaacttgaa gtgatgacag gctgccgcac
     3181 cgggcaaatt cgcttactgt atatcgcccc ggaacgcctg atgctggata actttcttga
     3241 gcatctggcg cactggaatc cggtgttatt agccgtcgat gaagcgcact gtatctccca
     3301 atggggtcac gatttccgcc cggaatatgc cgcgctcggt cagttgcgcc agcggttccc
     3361 gacgctgccg tttatggcgc tgaccgccac agccgacgac accacgcgcc aggatatcgt
     3421 gcgcctgctg gggctgaacg atccgctgat tcaaatcagc agttttgacc gtccgaatat
     3481 tcgctacatg ctgatggaga aattcaaacc gctcgatcag ttgatgcgct acgtgcagga
     3541 acagcgcggt aagtctggca ttatctactg caacagccgg gcgaaagtag aagacaccgc
     3601 tgcgcgcctg caaagcaggg gtattagcgc ggcggcctat catgccgggc tggaaaataa
     3661 tgtccgcgcc gacgtgcagg agaaattcca gcgcgatgat ctgcaaattg tggtggcgac
     3721 ggtggcgttc ggcatgggca tcaataaacc aaacgttcgc ttcgtggtcc actttgatat
     3781 tccgcgcaat atcgaatcct attatcagga aaccggtcgc gccgggcgtg atggcctgcc
     3841 tgcggaagcg atgctgtttt acgatccggc tgatatggcg tggctgcgcc gttgtctgga
     3901 agagaagccg caggggcagt tgcaggatat cgagcgccac aaactcaatg cgatgggcgc
     3961 gtttgccgaa gcgcaaactt gccgtcgtct ggtattgctg aactattttg gcgaagggcg
     4021 tcaggagccg tgcgggaact gcgatatctg cctcgatccg ccgaaacagt acgacggttc
     4081 aaccgatgct cagattgccc tttccaccat tggtcgtgtg aatcagcggt ttgggatggg
     4141 ttatgtggtg gaagtgattc gtggtgctaa taaccagcgt atccgcgact atggtcatga
     4201 caaactgaaa gtctatggta tgggccgtga taaaagccat gaacattggg tgagcgtgat
     4261 ccgccagctg attcatctcg gcctggtgac gcaaaatatt gcccagcatt ctgccctaca
     4321 actgacagag gccgcgcgcc cggtgctgcg cggcgaatcc tctttgcaac ttgccgtgcc
     4381 gcgtatcgtg gcgctcaaac cgaaagcgat gcagaaatcg ttcggcggca actatgatcg
     4441 caaactgttc gccaaattac gcaaactgcg taaatcgatt gccgatgaaa gtaatgtccc
     4501 gccgtacgtg gtgtttaacg acgcaacctt gattgagatg gctgaacaga tgccgatcac
     4561 cgccagcgaa atgctcagcg ttaacggcgt tgggatgcgc aagctggaac gctttggtaa
     4621 accgtttatg gcgctgattc gtgcgcatgt cgatggcgac gacgaagagt agtcagcagc
     4681 ataaaaaagt gccagtatga agactccgta aacgtttccc ccgcgagtca aatgtatgtt
     4741 gatgttattt ctcaccgtcg ccatggtgca cattgtggcg cttatgagcc ccggtcccga
     4801 tttctttttt gtctctcaga ccgctgtcag tcgttcccgt aaagaagcga tgatgggcgt
     4861 gctgggcatt acctgcggcg taatggtttg ggctgggatt gcgttgcttg gcctgcattt
     4921 gattatcgaa aaaatggcct ggctgcatac gctgattatg gtaggcggtg gcctgtatct
     4981 ctgctggatg ggttaccaga tgctacgcgg tgcactgaaa aaagaggctg tatctgcgcc
     5041 tgcaccacag gttgagctgg cgaaaagcgg gcgcagtttc ctgaaaggct tactgaccaa
     5101 tctcgccaat ccgaaagcga ttatctattt tggttcggta ttctcattgt ttgtcggcga
     5161 taacgttggc actaccgagc gctggggcat tttcgcgttg attattgtcg aaacactggc
     5221 gtggtttacc gtcgttgcca gtctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     5281 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     5341 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     5401 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     5461 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     5521 gcaatccttt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     5581 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     5641 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     5701 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taagatcaat tgcgccagcg
     5761 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca ctttaacact
     5821 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     5881 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     5941 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     6001 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     6061 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgcttaa aaaaacgatt
     6121 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     6181 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     6241 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     6301 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     6361 ggaactggcc tacgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     6421 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     6481 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     6541 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     6601 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     6661 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     6721 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     6781 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     6841 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     6901 gttagccggt gcgggtgatg acgccacgca aacgcttctc ttacaagctg aagaggaacg
     6961 cgtggtggat aaccgcatgc acgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     7021 tgtcgaagga ggacggccgt tggtaataaa aggtgcttac catgagattc tttttgaaaa
     7081 ggacgcaatg cgctcagtcg cgctccacgc catcgttgat tttttcaaca ggcataactc
     7141 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     7201 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaactc
     7261 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     7321 atgtggggca aattcgcgat aatctggaga ttaagtctta catgattacc tccaatggtg
     7381 cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg
     7441 ccagcgatct gtttggcgta gtcaacgaca atccggacat cattactaac gtttatcgcg
     7501 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     7561 tgttcaaata tgcgctgtat gagccgggat tactggagcc ggaaggcatc agcaaagtgt
     7621 ttttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgct
     7681 ggggcgatcg cgtcaacgtc agtttctcta ccttaacctg tctggaagtg atggcgggcg
     7741 gcgtgtcgaa aggccatgcg ctggaagcag tggcgaagaa actgggctac agcctgaagg
     7801 attgtattgc gtttggtgac gggatgaacg acgccgaaat gctgtcgatg gcggggaaag
     7861 gctgcattat gggcagtgcg caccagcgtc tgaaagatct gcatccggag ctggaagtta
     7921 ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat
     7981 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     8041 aagagttttc cattgtggcg ttacttatca tcaccacgat tctgtgggcc ttctccttta
     8101 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     8161 ttggcttggc ggcactcgtt tttctgccgt ttctgcgtac ccgtagcaat agcctgaaaa
     8221 cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt
     8281 tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc
     8341 tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgctt
     8401 ttagcgcctt gctggcggtg attggtgccg ggattattcg ctatgatcag gtcaccgacc
     8461 atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg
     8521 tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gtataacgcc tttgcgtggt
     8581 tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga
     8641 aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg
     8701 ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca
     8761 ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac
     8821 agccgcactg gccaacgttt attacaggcg cgctggtgat cctggcctca ctgtgggtgc
     8881 atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg
     8941 cgctgagcga ataaacgctt ccgtaactgg ctgacgctgc tcgccatcgc gcacggcggc
     9001 gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac cctggcgctc
     9061 aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatttg
     9121 aatcaacaat aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag
     9181 aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaattaata gcgtctcgct
     9241 ggcgagatct tccggtgtaa tacgcgtttt tgccgccagt ggatggtcag gtgctaacac
     9301 caggcgtact tcataatcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc
     9361 ggaggtcatc accagatcca gctcgccctg ttgcaacgcg ggctgcgggt caaacgtcac
     9421 gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttc ctaaagcggg
     9481 tgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg
     9541 ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg ccagttgcaa
     9601 caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa
     9661 gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa
     9721 cgttgccgca gcggctgcga gcgagccgca gttccgcaac gcttgtagcg ttttcaggtg
     9781 ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat
     9841 acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcat tcgtctttta
     9901 aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg
     9961 cgatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag
    10021 aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg
    10081 cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct
    10141 gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga
    10201 cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct
    10261 gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt
    10321 ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa
    10381 atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa
    10441 actgacttgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctga gccacaacgt
    10501 taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca
    10561 gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga
    10621 actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact
    10681 accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa
    10741 actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct
    10801 gcctgttcag gggctgcatg tggaccttgt acatggtaaa gataacgttg ttgaactgca
    10861 caagcgcctg ccttctgact ggctgctgtc tgcgggtctg atcaatggtc gtaacgtctg
    10921 gcgcgccgat cttactgaga aatatgcgca aattaaggac attgtcggca aacgtgattt
    10981 gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg
    11041 tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactggc
    11101 actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc
    11161 aattcaggcg cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc
    11221 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca
    11281 gcgtgcgcgt tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac
    11341 cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaacaacta
    11401 ccgcacgggc attgcggaac atatcaagca ggccattgtt gagcaggaac gtttgggact
    11461 ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca
    11521 cctcgacgga tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt
    11581 gaagccaccg attgtcattg gtgacattag ccgcccggca ccgattaccg tggagtgggc
    11641 gaagtatgcg caatcgctga ccgacaaacc ggtgaaaggg atgctgacgg ggccggtgac
    11701 catactctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat
    11761 tgcgctggcg ctgcgtgatg aagtggccga tctggaagcc gctggaattg gcatcatcca
    11821 gattgacgaa ccggcgctgc gcgaaggttt accgctgcgt cgtagcgact gggatgcgta
    11881 tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca
    11941 aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga ttgcggcgct
    12001 ggacgcagac gtcatcacca tcgaaacctc gcgttccgac atggagttgc tggagtcgtt
    12061 tgaagagttt gattatccaa atgaaatcgg tcctggcgtc tatgacattc actcgccaaa
    12121 cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc
    12181 agagcgcctg tgggtcaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg
    12241 cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcgggggt aaatccaaac
    12301 cgggtggtaa taccacccgg tcttttctca ttacagcgac ttcttcccac catactgctt
    12361 aaaccattcc agcatacgct gccagccatc tttcgcagat tcggcatgat agctcgggcg
    12421 ataatcagcg ttgaatgcat gcccggcatc cgggtacacg ataatctctg ctttcgcatt
    12481 agcagcccgc agcgcctggc gcatcgtttc aacgctctcc tgcggaatgc tgttatcctg
    12541 accaccatat aagccgagaa ccggcgcgtt aagatcggtt gcgatatcaa caggttgttt
    12601 cggtgaattc agcgacttgt cgcccgtcag tttgccgtac cacgccactg cggcttttag
    12661 ctgtggatta tgcgcggcat acagccaggt gatacgtcca ccccagcaga atccggtgat
    12721 cattaaacga tgaacatcgc cgccgttgcg ggaagcccaa ctggcgacat gatcgagatc
    12781 ggccagcacc tgcgagtcag gcacttttgc taccagaccg ctaagcaacg tggggatatc
    12841 ggcaaaatca ttcggatcgc cttcgcggaa gtaaagttca ggtgcgatag ccagataccc
    12901 ctccagcgcc agacggcgac aaatgtcgcg gatatgttca tgcacgccaa aaatttcctg
    12961 cactacaatg accactggca gtgggccatc gctttgcttt ggtctggcat ggtaagcagg
    13021 catgttatcc ccttgtgaag ggatagaggt gaagcccgcc acaatcgcgt cgtccggggt
    13081 ctgaacgatg gtcgaagcga gaggcgatgc agcaggtgca aatccagatt gttgtgttgt
    13141 tgccatggta ttctccgtac ccttataaaa atgttgcgca atgttaacta tagtcagcat
    13201 gcaacaaatc acattgcctg aatcggctca tcttttatgc agtcctgcag aatgaagagt
    13261 gatttatgtg atatgaatca cttttggtgg gtaaatttat gcaacgcatt tgcgtcatgg
    13321 tgatgagtat cacgaaaaaa tgttaaaccc ttcggtaaag tgtctttttg cttcttctga
    13381 ctaaaccgat tcacagagga gttgtatatg tccaagtctg atgtttttca tctcggcctc
    13441 actaaaaacg atttacaagg ggctacgctt gccatcgtcc ctggcgaccc ggatcgtgtg
    13501 gaaaagatcg ccgcgctgat ggataagccg gttaagctgg catctcaccg cgaattcact
    13561 acctggcgtg cagagctgga tggtaaacct gttatcgtct gctctaccgg tatcggcggc
    13621 ccgtctacct ctattgctgt tgaagagctg gcacagctgg gcattcgcac cttcctgcgt
    13681 atcggtacaa cgggcgctat tcagccgcat attaatgtgg gtgatgtcct ggttaccacg
    13741 gcgtctgtcc gtctggatgg cgcgagcctg cacttcgcac cgctggaatt cccggctgtc
    13801 gctgatttcg aatgtacgac tgcgctggtt gaagctgcga aatctattgg cgcgacaact
    13861 cacgttggcg tgacagtttc ttctgatacc ttctacccag gtcaggaacg ttacgatact
    13921 tactctggtc gcgtagttcg tcactttaaa ggttctatgg aagagtggca ggcgatgggc
    13981 gtaatgaact atgaaatgga atctgcaact ctgctgacca tgtgtgcaag tcagggcctg
    14041 cgtgccggta tggtagcggg tgttatcgtt aaccgcaccc agcaagagat cccgaatgct
    14101 gagacgatga aacaaaccga aagccatgcg gtgaaaatcg tggtggaagc ggcgcgtcgt
    14161 ctgctgtaat tctcttctcc tgtctgaagg ccgacgcgtt cggccttttg tatttttgcg
    14221 tagcgcctcg caggaaatgc ctttccaact ggacgtttgt acagcacaat tctattttgt
    14281 gcgggtaagt tgttgcgtca ggaggcgttg tggatttctc aatcatggtt tacgcagtta
    14341 ttgcgttggt gggtgtggca attggctggc tgtttgccag ttatcaacat gcgcagcaaa
    14401 aagccgagca attagctgaa cgtgaagaga tggtcgcgga gttaagcgcg gcaaaacaac
    14461 aaattaccca aagcgagcac tggcgtgcag agtgtgaatt gctcaataac gaagtgcgca
    14521 gcctgcaaag tattaacacc tctctggagg ccgatctgcg tgaagtaacc acgcggatgg
    14581 aagccgcaca gcaacatgct gacgataaaa ttcgccagat gattaacagc gagcagcgcc
    14641 tcagtgagca gtttgaaaac ctcgccaacc gtatttttga gcacagcaat cgccgggttg
    14701 atgagcaaaa ccgtcagagt ctgaacagcc tgttgtcgcc gctacgtgaa caactggacg
    14761 gtttccgccg tcaggttcag gacagcttcg gtaaagaagc acaagaacgc cataccctga
    14821 cccacgaaat tcgcaatctc cagcaactca acgcgcaaat ggcccaggaa gcgatcaacc
    14881 tgacgcgcgc gctgaaaggc gacaataaaa cccagggcaa ctggggcgag gtagtattga
    14941 cgcgggtgct ggaggcttcc ggtctgcgtg aagggtatga atatgaaacc caggtcagca
    15001 tcgaaaatga cgcccgctcg cggatgcagc cggatgtcat cgtgcggctg ccgcagggaa
    15061 aagatgtggt gatcgacgcc aaaatgacgc tggtcgccta tgaacgctat tttaacgccg
    15121 aagacgacta cacccgcgaa agcgcgctac aggaacatat cgcgtcggtg cgtaaccata
    15181 tccgtttgct gggacgcaaa gattatcaac agctgccggg gctgcgaact ctggattacg
    15241 tgctgatgtt tattcccgtt gaacccgctt ttttactggc gcttgaccgc cagccggagc
    15301 tgatcaccga agcgttgaaa aacaatatca tgctggttag cccgactacg ctgctggtgg
    15361 cgttgcgtac tattgccaac ctgtggcgtt atgagcatca aagccgtaac gcccagcaaa
    15421 tcgccgatcg tgccagcaag ctgtacgaca agatgcgttt gttcatcgat gacatgtccg
    15481 cgattggtca aagtctcgac aaagcgcagg ataactaccg ccaggcaatg aaaaaactct
    15541 cttcaggtcg cggaaatgtg ctggcgcagg cagaagcgtt tcgcggttta ggagtagaaa
    15601 ttaaacgcga gattaatccg gatttggctg aacaggcggt gagccaggat gaagagtatc
    15661 gacttcggtc ggttccggag cagccgaatg atgaagctta tcaacgcgat gatgaatata
    15721 atcagcagtc gcgctagccc attaggggta gttaagccgg gtagaaatct agggcatcga
    15781 cgcccaatct gttacacttc tggaacaatt ttttgatgag caggcattga gatggtggat
    15841 aagtcacaag aaacgacgca ctttggtttt cagaccgtcg cgaaggaaca aaaagcggat
    15901 atggtcgccc acgttttcca ttccgtggca tcaaaatacg atgtcatgaa tgatttgatg
    15961 tcatttggta ttcatcgttt gtggaagcga ttcacgattg attgcagcgg cgtacgccgt
    16021 gggcagaccg tgctggatct ggctggtggc accggcgacc tgacagcgaa attctcccgc
    16081 ctggtcggag aaactggcaa agtggtcctt gctgatatca atgaatccat gctcaaaatg
    16141 ggacgcgaga agctgcgtaa tatcggtgtg attggcaacg ttgagtatgt tcaggcgaac
    16201 gctgaggcgc tgccgttccc ggataacacc tttgattgca tcaccatttc gtttggtctg
    16261 cgtaacgtca ccgacaaaga taaagcactg cgttcaatgt atcgcgtgct gaaacccggc
    16321 ggccgcctgc tggtgcttga gttctcgaag ccaattatcg agccgctgag caaagcctat
    16381 gatgcatact ccttccatgt gctgccgcgt attggctcac tggtcgcgaa cgacgccgac
    16441 agctaccgtt atctggcaga atccatccgt atgcatcccg atcaggatac cctgaaagcc
    16501 atgatgcagg atgccggatt cgaaagtgtc gactactaca atctgacggc aggggttgtg
    16561 gcgctgcatc gtggttataa gttctgacag gagaccggaa atgcctttta aacctttagt
    16621 gacggcagga attgaaagtc tgctcaacac cttcctgtat cgctcacccg cgctgaaaac
    16681 agcccgctcg cgtctgctgg gtaaagtatt gcgcgtggag gtaaaaggct tttcgacgtc
    16741 attgattctg gtgttcagcg aacgccaggt tgatgtactg ggcgaatggg caggcgatgc
    16801 tgactgcacc gttatcgcct acgccagtgt gttgccgaaa cttcgcgatc gccagcagct
    16861 taccgcactg attcgcagtg gtgagctgga agtgcagggc gatattcagg tggtgcaaaa
    16921 cttcgttgcg ctggcagatc tggcagagtt cgaccctgcg gaactgctgg ccccttatac
    16981 cggtgatatc gccgctgaag gaatcagcaa agccatgcgc ggaggcgcaa agttcctgca
    17041 tcacggcatt aagcgccagc aacgttatgt ggcggaagcc attactgaag agtggcgtat
    17101 ggcacccggt ccgcttgaag tggcctggtt tgcggaagag acggctgccg tcgagcgtgc
    17161 tgttgatgcc ctgaccaaac ggctggaaaa actggaggct aaatgacgcc aggtgaagta
    17221 cggcgcctat atttcatcat tcgcactttt ttaagctacg gacttgatga actgatcccc
    17281 aaaatgcgta tcaccctgcc gctacggcta tggcgatact cattattctg gatgccaaat
    17341 cggcataaag acaaactttt aggtgagcga ctacgactgg ccctgcaaga actggggccg
    17401 gtttggatca agttcgggca aatgttatca acccgccgcg atctttttcc accgcatatt
    17461 gccgatcagc tggcgttatt gcaggacaaa gttgctccgt ttgatggcaa gctggcgaag
    17521 cagcagattg aagctgcaat gggcggcttg ccggtagaag cgtggtttga cgattttgaa
    17581 atcaagccgc tggcttctgc ttctatcgcc caggttcata ccgcgcgatt gaaatcgaat
    17641 ggtaaagagg tggtgattaa agtcatccgc ccggatattt tgccggtcat taaagcggat
    17701 ctgaaactta tctaccgtct ggctcgctgg gtgccgcgtt tgctgccgga tggtcgccgt
    17761 ctgcgcccaa ccgaagtggt gcgcgagtac gaaaagacct tgattgatga actgaatttg
    17821 ctgcgggaat ctgccaacgc cattcagctt cggcgcaatt ttgaagacag cccgatgctc
    17881 tacatcccgg aagtttaccc tgactattgt agtgaaggga tgatggtgat ggagcgcatt
    17941 tacggcattc cggtgtctga tgttgcggcg ctggagaaaa acggcactaa catgaaattg
    18001 ctggcggaac gcggcgtgca ggtgttcttc actcaggtct ttcgcgacag ctttttccat
    18061 gccgatatgc accctggcaa catcttcgta agctatgaac acccggaaaa cccgaaatat
    18121 atcggcattg attgcgggat tgttggctcg ctaaacaaag aagataaacg ctatctggca
    18181 gaaaacttta tcgccttctt taatcgcgac tatcgcaaag tggcagagct acacgtcgat
    18241 tctggctggg tgccaccaga taccaacgtt gaagagttcg aatttgccat tcgtacggtc
    18301 tgtgaaccta tctttgagaa accgctggcc gaaatttcgt ttggacatgt actgttaaat
    18361 ctgtttaata cggcgcgtcg cttcaatatg gaagtgcagc cgcaactggt gttactccag
    18421 aaaaccctgc tctatgtcga aggggtagga cgccagcttt atccgcagct cgatttatgg
    18481 aaaacagcga agcctttcct ggagtcgtgg attaaagatc aggtcggtat tcctgctctg
    18541 gtgagagcat ttaaagaaaa agcgccgttc tgggtcgaaa aaatgccaga actgcctgaa
    18601 ctggtttacg acagtttgcg ccagggcaag tatttacagc acagtgttga taagattgcc
    18661 cgcgagcttc agtcaaatca tgtacgtcag ggacaatcgc gttattttct cggaattggc
    18721 gctacgttag tattaagtgg cacattcttg ttggtcagcc gacctgagtg ggggctgatg
    18781 cccggctggt taatggcagg tggtctgatc gcctggtttg tcggttggcg caaaacacgc
    18841 tgattttttc atcgctcaag gcgggccgtg taacgtataa tgcggctttg tttaatcatc
    18901 atctaccaca gaggaacatg tatgggtggt atcagtattt ggcagttatt gattattgcc
    18961 gtcatcgttg tactgctttt tggcaccaaa aagctcggct ccatcggttc cgatcttggt
    19021 gcgtcgatca aaggctttaa aaaagcaatg agcgatgatg aaccaaagca ggataaaacc
    19081 agtcaggatg ctgattttac tgcgaaaact atcgccgata agcaggcgga tacgaatcag
    19141 gaacaggcta aaacagaaga cgcgaagcgc cacgataaag agcaggtgta atccgtgttt
    19201 gatatcggtt ttagcgaact gctattggtg ttcatcatcg gcctcgtcgt tctggggccg
    19261 caacgactgc ctgtggcggt aaaaacggta gcgggctgga ttcgcgcgtt gcgttcactg
    19321 gcgacaacgg tgcagaacga actgacccag gagttaaaac tccaggagtt tcaggacagt
    19381 ctgaaaaagg ttgaaaaggc gagcctcact aacctgacgc ccgaactgaa agcgtcgatg
    19441 gatgaactac gccaggccgc ggagtcgatg aagcgttcct acgttgcaaa cgatcctgaa
    19501 aaggcgagcg atgaagcgca caccatccat aacccggtgg tgaaagataa tgaagctgcg
    19561 catgagggcg taacgcctgc cgctgcacaa acgcaggcca gttcgccgga acagaagcca
    19621 gaaaccacgc cagagccggt ggtaaaacct gctgcggacg ctgaaccgaa aaccgctgca
    19681 ccttcccctt cgtcgagtga taaaccgtaa acatgtctgt agaagatact caaccgctta
    19741 tcacgcatct gattgagctg cgtaagcgtc tgctgaactg cattatcgcg gtgatcgtga
    19801 tattcctgtg tctggtctat ttcgccaatg acatctatca cttggtatcc gcgccattga
    19861 tcaagcagtt gccgcaaggt tcaacgatga tcgccaccga cgtggcctcg ccgttcttta
    19921 cgccgatcaa gctgaccttt atggtgtcgc tgattctgtc agcgccggtg attctctatc
    19981 aggtgtgggc atttatcgcc ccagcgctgt ataagcatga acgtcgcctg gtggtgccgc
    20041 tgctggtttc cagctctctg ctgttttata tcggcatggc attcgcctac tttgtggtct
    20101 ttccgctggc atttggcttc cttgccaata ccgcgccgga aggggtgcag gtatccaccg
    20161 acatcgccag ctatttaagc ttcgttatgg cgctgtttat ggcgtttggt gtctcctttg
    20221 aagtgccggt ggcaattgtg ctgctgtgct ggatggggat tacctcgtca gaagacttac
    20281 gcaaaaaacg cccgtatgtg ctggttggtg cattcgttgt cgggatgttg ctgacgccgc
    20341 cggatgtctt ctcgcaaacg ctattggcga tcccgatgta ctgtctgttt gaaatcggtg
    20401 tcttcttctc acgcttttac gttggtaaag ggcgaaatcg ggaagaggaa aacgacgctg
    20461 aagcagaaag cgaaaaaact gaagaataaa ttcaaccgcc cgtcagggcg gttgtcatat
    20521 ggagtacagg atgtttgata tcggcgttaa tttgaccagt tcgcaatttg cgaaagaccg
    20581 tgatgatgtt gtagcgcgcg cttttgacgc gggagttaat gggctactca tcaccggcac
    20641 taacctgcgt gaaagccagc aggcgcaaaa gctggcgcgt cagtattcgt cctgttggtc
    20701 aacggcgggc gtacatcctc acgacagcag ccagtggcaa gctgcgactg aagaagcgat
    20761 tattgagctg gccgcgcagc cagaagtggt ggcgattggt gaatgtggtc tcgactttaa
    20821 ccgtaacttt tcgacgccgg aagagcagga acgcgctttt gttgctcagc tacgcattgc
    20881 cgcagaatta aacatgccgg tatttatgca ctgtcgcgat gcccacgagc ggtttatgac
    20941 attgctggag ccgtggctgg ataaactgcc tggtgcggtt cttcattgct ttaccggcac
    21001 acgcgaagag atgcaggcgt gcgtggcgcg tggaatttat atcggcatta ccggttgggt
    21061 ttgcgatgaa cgacgcgggc tggagctgcg ggaattgttg ccgttgattc cggcggagaa
    21121 attgctgatc gaaacagatg cgccgtatct gctcccgcgc gatctcacgc caaagccatc
    21181 atcccggcgc aacgagccag cccatctgcc ccatattttg caacgtattg cgcactggcg
    21241 tggagaagat gccgcatggc tggctgccac cacggatgcc aatgtcaaaa cactgtttgg
    21301 gattgcgttt tagagtttgc ggaactcggt gttcttcaca ctgtgcttaa tctctttatt
    21361 aataagatta agcaatagca tggagcgagc ctcaccatcg ggttcggtga aaatggcctg
    21421 aaagccttcg aacgcgcctt cggtaataat caccttatca cccggataag ggggggccgg
    21481 atcgacaatg tctttcggtt tatataccga tagctgatga attaccgccg atgggactat
    21541 cgctggcgac gcgccaaagc gcacaaagtg gctgacgcca cgcgtagcgc tgatggtcgt
    21601 ggtatgaatc acttctggat caaattccac aaacaggtag ttagggaata acggttcact
    21661 gactgcggta cgtttacccc gtacgatttt ttccagggtg atcatcggag cgaggcaatt
    21721 aaccgcctgt ctctcaaggt gttcctgggc acgttgaagt tgcccgcgct tgcagtacag
    21781 taaataccag gattgcataa tgactcttat ccgtttaatc ggggcgcaag gatagcaaaa
    21841 gctttacgct aagttaatta tattccccgg tttgcgttat accgtcagag ttcacgctaa
    21901 tttaacaaat ttacagcatt gcaaagatga acgccgtata atgggcgcag attaagaggc
    21961 tacaatggac gccatgaaat ataacgattt acgcgacttc ctgacgctgc ttgaacagca
    22021 gggtgagcta aaacgtatca cgctcccggt ggatccgcat ctggaaatca ctgaaattgc
    22081 tgaccgcact ctgcgtgctg gtgggcctgc gctgttgttc gaaaacccta aaggctactc
    22141 aatgccggtg ctgtgcaacc tgttcggtac gccaaagcgc gtggcgatgg gcatggggca
    22201 ggaagatgtt tcggcgctgc gtgaagttgg taaattattg gcgtttctga aagagccgga
    22261 gccgccaaaa ggtttccgcg acctgtttga taaactgccg cagtttaagc aggtgttaaa
    22321 catgccgaca aagcgactgc gtggtgcgcc ctgccaacaa aaaatcgtct ctggcgatga
    22381 cgtcgatctc aatcgcattc ccattatgac ctgctggccg gaagatgccg cgccgctgat
    22441 tacctggggg ctgaccgtaa cgcgcggccc gcataaagag cggcagaatc tgggcattta
    22501 tcgccagcag ctgattggta aaaacaaact gattatgcgc tggctgtcgc atcgcggcgg
    22561 cgcgctggat tatcaggagt ggtgtgcggc gcatccgggc gaacgtttcc cggtttctgt
    22621 ggcgctgggt gccgatcctg ccacgattct cggtgcagtc acccccgttc cggatacgct
    22681 ttcagagtat gcgtttgccg gattgctacg cggcaccaaa accgaagtag taaagtgtat
    22741 ttccaatgac ctcgaagtgc cagccagtgc cgaaatcgtc ctggaagggt atatcgatcc
    22801 tggtgagatg gcgccggaag ggccgtatgg tgaccacaca ggttactata atgaagtcga
    22861 taatttcccg gtgtttaccg tgacgcatat tacccagcgt gaagatgcga tttaccattc
    22921 cacctatacc gggcgtccgc cagatgagcc cgcggtactg ggcgtggcgt tgaacgaagt
    22981 gtttgtaccg attctgcaaa aacagttccc ggaaattgtc gatttttacc tgccgccgga
    23041 aggctgttct tatcgtctgg cggtagtgac gatcaaaaaa cagtacgccg gacacgcgaa
    23101 gcgcgtcatg atgggcgtct ggtcgttctt acgccagttt atgtacacta aatttgtgat
    23161 cgtttgcgat gatgacgtca acgcacgcga ctggaacgat gtgatttggg cgattaccac
    23221 ccgtatggac ccggcgcggg atactgttct ggtagaaaat acgcctattg attatctgga
    23281 ttttgcctcg cctgtctctg ggctgggttc aaaaatgggg ctggatgcca cgaataaatg
    23341 gccgggggaa acccagcgtg aatggggacg tcccatcaaa aaagatccag atgttgtcgc
    23401 gcatattgac gccatctggg atgaactggc tatttttaac aacggtaaaa gcgcctgatg
    23461 cgcgtttgtt ttgccctatt tatcgatccg acagagaaag cgcatgacaa ccttaagctg
    23521 taaagtgacc tcggtagcag ctatcacgga taccgtatat cgtgtccgca tcgtgccaga
    23581 cgcggccttt tcttttcgtg ctggtcagta tttgatggta gtgatggatg agcgcgacaa
    23641 acgtccgttc tcaatggcct cgacgccgga tgaaaaaggg tttatcgagc tgcatattgg
    23701 cgcttctgaa atcaaccttt acgcgaaagc agtcatggac cgcatcctca aagatcatca
    23761 aatcgtggtc gaccttcccc acggagaagc gtggctgcgc gatgatgaag agcgtccgat
    23821 gattttgatt gcgggcggca ccgggttctc ttatgcccgc tcgattttgc tgacagcgtt
    23881 ggcgcgtaac ccaaaccgtg atatcaccat ttactggggc gggcgtgaag agcagcatct
    23941 gtatgatctc tgcgagcttg aggcgctttc gttaaagcac cttggtctgc aagtggtgcc
    24001 ggtggttgaa caaccggaag caggctggcg tgggcgtact ggtaccgtgt taacggcggt
    24061 attgcaggat catggtacgc tggcagagca tgatatctat attgccggac gttttgagat
    24121 ggcgaaaatt gcccgtgacc tgttttgcag tgagcgtaat gcgcgggaag atcgcctgtt
    24181 tggcgatgcg tttgcattta tctgagatat aaaaaaaccc gcccctgaca ggcgggaaga
    24241 acggcaacta aaactttatt cagtggcatt tagatctatg tcgcatccgg caattggtgc
    24301 acgatgcctg atgcgacgct ggcgcgtctt atcaggccta cattggtgcc ggatcggtag
    24361 accggataag gcgttcacgc cgcatccggc aagtggttaa acccgctcaa acaccgtcgc
    24421 aataccctga cccagaccga tacacatcgt cgccagacca aactgaacgt ctttgcgttc
    24481 catcagattc agcagcgtgg tgctgatacg cgcaccggaa caacccagcg gatgacccag
    24541 cgcgatcgca ccaccattga ggttgatctt ctcgtcaatc tgctccatta gcccgagatc
    24601 tttaatgcac ggcaggatct gcgcggcaaa ggcttcgttc atctcaaaca cgccgatatc
    24661 gctggcagaa agccccgctt ttttcagcgc cagcttcgag gccggaaccg ggccgtaacc
    24721 cataatcgat gggtcacaac caacgaccgc catcgaacgc acacgagcgc gcggcttaag
    24781 acctaattca tgggcgcggc tttcactcat caccagcatg gcagctgcgc catcggaaag
    24841 tgcagaagat gtgcccgccg ttaccgtacc gtttactgga tcaaacgccg gacgcagcgt
    24901 ggcgagggct tccacggtgg tttccgggcg aatcacttcg tcgtaattaa actgcttcag
    24961 gacgccgtcg gcatcgtgac caccagttgg gatgatttca tttttaaatg cggccgactg
    25021 cgtggcggcc caggcgcggg cgtgtgaccg cgcggcaaag gcatcctgca tttcacggct
    25081 gataccgtgc atacgcgcca gcatttctgc cgttaagccc atcatgcccg ccgctttggc
    25141 gacattgcgg ctcaggccgg ggtgaaaatc gacgccgtga ctcatcggca catggcctat
    25201 atgctccacg ccgccaacca gacatgcctg cgtatcgcca gtcatgatca ttcgtgctgc
    25261 gtcatgcagt gcctgcatgg atgaaccaca caagcgattg acggtaaccg ccgggacaga
    25321 gtgcggcact tctgccagca gcgccgcgtt acgggcgatg ttaaaaccct gctccagcgt
    25381 ctgttgcaca caaccccagt aaatatcgtc gagggccgcc gcttccagcg ccgggttacg
    25441 cgccagcagg ctacgcatta aatgagcgga gagatcttcc gcacgcacgt tacgaaaagc
    25501 accgcccttc gaacggccca tcggggtgcg aattgcatcg acaatgacaa cctgttccat
    25561 tgtgactcct taagccgttt tcaggtcgcc aaccggacgg gctggctcaa ccggaggata
    25621 gtacggttcg ttatgacgcg ctttattacg cagaccttcc ggcacttcat acagcgggcc
    25681 gaggtgctga tattgctgtg ccatatcgag gtattttgcg ctaccgaggg tgtccagcca
    25741 gcggaacgcg ccgccgtgga acggagggaa gcccaggccg tagaccagcg ccatatccgc
    25801 ttccgccgga gtggcgataa tgccctcttc cagacagcgc accacttcgt tgaccatcgg
    25861 gatcatcatg cgggcgataa tctcttcttc gctgaaatcg cgcttcggtt ggctcacttc
    25921 tgccagcagg tcttcaacgg cggcgtcttc ttctttcttc ggcttacctt tgctgtcttc
    25981 tttataacgc cagaaaccga ggccgttctt ctgaccaaag cggttggcat caaacagcgc
    26041 gtcgatggca tcgcggtaat ctttctgcat ccgctgcggg aagcctgctg ccatgacagc
    26101 ctgagcgtga tgcgcggtat caatgcccac aacgtccagc agatatgccg ggcccatcgg
    26161 ccagccaaac tgtttttcca tcactttgtc gatcttgcgg aaatccgcgc cgtcgcgcag
    26221 cagctggctg aaaccggcga aatacgggaa cagcacgcgg ttaacaaaga agccggggca
    26281 gtcgttaacc acaatcggcg tcttgcccat cttgctcgcc caggcgacaa ctttcgcgat
    26341 ggtttcgtcg gagcttttct cgccgcgaat aatttctacc aacggcattc ggtggaccgg
    26401 gttaaagaag tgcatcccgc agaagttttc cgggcgttcc agcgcgttgg ccagttcgct
    26461 gataggaatg gttgaagtgt tagacgccag cacggtatcc tggcgtactt tttgttcggt
    26521 ttctgccagt acggcttttt tcactttcgg gttttcaaca accgcttcta ccacaatatc
    26581 cacgcggtca aatccggcgt agtcgagcgt tgggtggatc gtggagatca cgccagccag
    26641 tttcagacca tcgattttgc cgcgctcaag ctgcttgttc agcagtttcg cggcttcggt
    26701 catgccgagg gttaacgact tgtcgttgat atctttcatg acaaccggca cgcctttcca
    26761 cgcggactgg taagcgatac cgccacccat gatgcctgcg cccagcacag cagcctgttt
    26821 cggggtttca acgtctttgg tgagtttctt cgctttgcct tttacatatt gatcgttaag
    26881 gaaaatgccg accagtgcgc gagcttcgtt ggtatgcgcc agcgggacaa aacttttgtt
    26941 ttccagattt aaggcttctt cacgaccaaa acgggccgca gcttcaatgg tttttactgc
    27001 ggtgatgggg gccggataat gtttccccgc cgtttgcgcg accattcctt tagcgatggt
    27061 gaagctcatg gtggcttcaa tcttgctcag ttttagcggt tcctgcttcg gctgacgttt
    27121 cgctttccag tcgaggtcgc cgttaatggc ctggcgtaaa atcgccatcg ccccttcaat
    27181 cagtttttct gctttgacta cgccatccac cagaccgatt ttcagcgcct gatccgcgcc
    27241 gacatcttta ccggcggcaa tgatttccag tgcactgtca gcgcccagca tacgtggcat
    27301 acgtacagaa ccgccaaagc ccggcatgat gcccagtttg gtttccggca gaccgatgcg
    27361 cagatccggc gtcgccagac gataatcggt cgccagcacg cattcgcagc caccacccag
    27421 cgcatagccg ttgacggcag caatggtcgg caccggcaga tcttccaggc gattaaacac
    27481 gctattggca aagtgcagcc actgacttaa ctgttcttca ggaacgagga acagggacaa
    27541 aaattcggtg atatcagcac cgacgataaa ggctgctttg ttcgaacgca gcagcagccc
    27601 ttttagatct gattgctgtt ccagcacgcc gatggcctcg ccgaggctgg cgacggtcgc
    27661 agtgtcgagt ttattaactg aacctggggc atcaaatacc agttcggcaa tgccatcttc
    27721 cagccagtca aggtacaggg tgtcgccttt gtaaagcatg tcagtctcct gaatccgcaa
    27781 ggtaatctgg tcgtaccaga tgcatcggag tgtgtgtttt gtgttaaaaa tatgcaaata
    27841 aaagattaaa gaaatgccga tctgatcacg ctcggcagaa atcacgctct ggatgaacga
    27901 tgtgctaaga tgcggagact taaggtcaaa aaaacagaag ggtaaaaaat ggaatcactg
    27961 gcctcgctct ataaaaatca tatagctacc ttacaagaac ggactcgcga tgcgctggcg
    28021 cgcttcaagc tggatgcatt acttattcac tccggcgagc tgttcaacgt ttttctcgac
    28081 gatcatccct atccgtttaa agtgaacccg caattcaaag cgtgggtgcc ggtaactcag
    28141 gtgccaaact gctggttgct ggtggatggc gtgaacaagc cgaaactgtg gttctatctg
    28201 ccggttgatt actggcacaa cgtcgaaccg ctgccgacct ccttctggac tgaagatgtg
    28261 gaagtgatcg cgctgccgaa agccgatggc attggtagcc tgctgcctgc tgcgcgcggc
    28321 aatatcggtt atatcggtcc ggtgccggaa cgtgcgctgc aactgggtat tgaggccagc
    28381 aacatcaacc cgaaaggggt gatcgactac ctgcattatt accgctcctt caaaaccgag
    28441 tacgaactgg cctgtatgcg tgaagcgcag aaaatggcgg tcaatggtca tcgtgcggca
    28501 gaagaagcgt tccgttctgg catgagcgag ttcgatatca atattgccta tctgaccgcg
    28561 accggtcatc gtgataccga cgtaccttac agcaacattg tggcgcttaa cgaacacgct
    28621 gcggtgttgc attacaccaa actggaccac caggcaccgg aagagatgcg cagcttcctg
    28681 ctggatgccg gggccgaata taacggctat gcggctgacc tgacccgtac ctggtcggca
    28741 aaaagcgaca acgactacgc acagctggtg aaagacgtaa atgatgaaca actggcgctg
    28801 atcgccacca tgaaagcagg cgtcagctat gtggattacc acatccagtt ccatcagcgc
    28861 atcgccaaac tgctgcgtaa acatcaaatc atcaccgata tgagtgaaga agcgatggtc
    28921 gaaaacgatc tcaccggacc gtttatgccg cacggtattg gccatccgct gggcctgcag
    28981 gtgcatgacg tcgccggttt tatgcaggat gatagcggta cacacctcgc ggcaccggca
    29041 aaatatccgt acctgcgcta cacccgtatt ctccagccgg gcatggtgtt aaccatcgaa
    29101 ccgggtatct acttcattga atcgcttctg gcaccgtggc gtgaagggca gttcagcaag
    29161 cacttcaact ggcagaaaat tgaagcactg aaaccgttcg gcggcattcg tatcgaagac
    29221 aacgtggtga tccacgaaaa caacgtggaa aacatgaccc gggatctgaa actggcgtga
    29281 tggaaagctg gttaattcct gcggcaccgg tcacggtcgt tgaagagatc aaaaagagcc
    29341 gcttcataac gatgttggcg cataccgatg gcgttgaggc ggcgaaagcg tttgttgaat
    29401 cggtgcgggc agaacacccc gatgcccgcc accattgcgt ggcgtgggtg gcgggtgcgc
    29461 cggatgattc tcaacagctg ggtttctctg acgacgggga gccggcggga acggcaggta
    29521 aaccgatgct cgcccagcta atgggcagcg gcgtcgggga aattaccgct gtggtagtgc
    29581 gctactacgg cggcatattg cttggcaccg gtgggttagt gaaagcgtat ggcggcggcg
    29641 tgaatcaggc gctgcgccag ctaacgaccc aacgcaagac gccattaacc gaatatactt
    29701 tgcaatgtga atatagtcag ttaaccggca ttgaagcgtt gctggggcag tgtgacggca
    29761 aaattatcaa cagtgattat caggcattcg ttctgctgcg ggtggcgctt ccggcggcga
    29821 aagtggctga attttccgca aagctggcgg attttagccg tggttcattg caattgttag
    29881 cgattgaaga ataatcccca cttcgttttg cagaactaag gaagcggcag agatgcattt
    29941 tcgcgccatt acccgaatcg ttggactact ggtcatctta ttttcaggga ccatgattat
    30001 ccctgggctg gtagcactca tctaccggga tggagcgggc cgcgctttta cccagacctt
    30061 ttttgtcgcc ctcgccattg gctctatgct gtggtggccg aaccgcaaag agaagggcga
    30121 actgaaatcc cgtgaggggt ttctgatagt ggtgctgttt tggaccgtgc tgggtagtgt
    30181 cggtgcgctc ccttttatct tctcggaaag cccgaacctc acgattaccg atgcgttttt
    30241 tgaatctttc tctggcctga ccaccaccgg ggccactacg ctggtggggc tggattcgct
    30301 ccctcatgcc atcctctttt atcgccagat gctgcaatgg tttggcggga tggggatcat
    30361 cgtgttagcg gttgcgatac tgcctatcct cggcgtgggt gggatgcagc tctatcgcgc
    30421 agaaatgccc ggcccgctga aagataacaa aatgcgcccg cgaattgcgg aaacggcgaa
    30481 aactctgtgg ttgatttatg tcttgctgac cgtcgcctgt gcgctggcgt tgtggtttgc
    30541 cggaatggat gcctttgatg ccatcggcca tagctttgcg actatcgcta ttggcggctt
    30601 ctcgacacat gatgccagta tcggttattt cgacagtccg actattaaca ctatcattgc
    30661 tatcttcctg ctgatctccg gctgtaacta cggtctgcac ttttcactgt taagtgggcg
    30721 tagtctgaag gtttattggc gcgatccgga atttcgcatg tttatcggcg tacagtttac
    30781 gctggtggtt atttgtacac tcgtactgtg gtttcataat gtctacagtt cggcgctgat
    30841 gacaattaac caggcgtttt tccaggtggt atcgatggcg acaaccgccg ggtttacgac
    30901 tgacagcatt gcccgctggc cgctcttttt gccggtactg cttttatgtt cagcttttat
    30961 cggcggttgc gccgggtcaa cgggcggtgg cctgaaagtg atccgcatcc tgctgctgtt
    31021 taagcagggg aaccgtgagc tgaaacgact ggtgcatccg aacgccgtct atagcattaa
    31081 gctggggaat cgcgcactgc cggaacgtat cctcgaagcc gtttggggat ttttctccgc
    31141 ctatgcattg gtgtttattg tcagtatgct ggcgattatc gccacgggcg tggatgattt
    31201 ttctgccttt gcctcagttg ttgcgacatt gaataacctg gggccagggc ttggcgtggt
    31261 tgctgataac tttaccagta tgaacccggt ggctaaatgg atcctgattg ccaacatgct
    31321 gtttggtcgt ctcgaggtct ttacattact ggtgctcttt accccgactt tctggcgtga
    31381 atgatggagt aatacgtgaa aacattaatt cttttctcaa caagggacgg acaaacgcgc
    31441 gagattgcct cctacctggc ttcggaactg aaagaactgg ggatccagac ggatgtcgcc
    31501 aatgtgcacc gcattgaaga accacaatgg gaaaactatg accgtgtggt cattggtgct
    31561 tctattcgct atggtcacta ccactctgcg ttccaggaat ttgtcaaaaa acatgcgacg
    31621 cgactgaatt cgatgccgag cgccttttat tccgtaaacc tggtggcgcg caaaccggag
    31681 aagcgtactc cacagaccaa cagctacgcg cggaagtttc tgatgaactc gcaatggcgt
    31741 cccgatcgct gcgcggtcat tgccggggcg ctgcgttacc cacgttatcg ctggtacgac
    31801 cgttttatgc tcaagctgat tatgaagatg tcaggcggtg aaacggatac gcgcaaagaa
    31861 gttgtctata ccgattggga gcaggtggcg aatttcgccc gagaaatcgc ccatttaacc
    31921 gacaaaccga cgctgaaata agcataaaga ataaaaaatg cgcggtcaga aaattatttt
    31981 aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg acacggaaca
    32041 acggcaaaca cgcagccggg tcagcggggt tctcctgaga attccgacag agaaagcaaa
    32101 aataaatgct tgactttgta gcgggaaagc gtattatgaa caccccgcgc
//
LOCUS       DAZXUL010000041        31125 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZXUL010000041 DAZXUL010000000
VERSION     DAZXUL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31125
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.041"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            389..2047
                     /locus_tag="ACH3L1_001869"
     CDS             389..2047
                     /locus_tag="ACH3L1_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFT4076622.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="ACH3L1_001870"
     CDS             complement(2044..3000)
                     /locus_tag="ACH3L1_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFT4076623.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="ACH3L1_001871"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="ACH3L1_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFT4076624.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="ACH3L1_001872"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="ACH3L1_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFT4076625.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACH3L1_001873"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACH3L1_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFT4076626.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6656..6730
                     /gene="yljB"
                     /locus_tag="ACH3L1_001874"
     CDS             6656..6730
                     /gene="yljB"
                     /locus_tag="ACH3L1_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFT4076627.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="ACH3L1_001875"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="ACH3L1_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFT4076628.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="ACH3L1_001876"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="ACH3L1_001876"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFT4076629.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9799..9886)
                     /locus_tag="ACH3L1_001877"
     tRNA            complement(9799..9886)
                     /locus_tag="ACH3L1_001877"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9850..9852),aa:Ser,seq:gga)
     gene            complement(10140..10358)
                     /gene="infA"
                     /locus_tag="ACH3L1_001878"
     CDS             complement(10140..10358)
                     /gene="infA"
                     /locus_tag="ACH3L1_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFT4076630.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10643..11347)
                     /gene="aat"
                     /locus_tag="ACH3L1_001879"
     CDS             complement(10643..11347)
                     /gene="aat"
                     /locus_tag="ACH3L1_001879"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFT4076631.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11389..13110)
                     /gene="cydC"
                     /locus_tag="ACH3L1_001880"
     CDS             complement(11389..13110)
                     /gene="cydC"
                     /locus_tag="ACH3L1_001880"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFT4076632.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13111..14877)
                     /gene="cydD"
                     /locus_tag="ACH3L1_001881"
     CDS             complement(13111..14877)
                     /gene="cydD"
                     /locus_tag="ACH3L1_001881"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFT4076633.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15000..15965)
                     /gene="trxB"
                     /locus_tag="ACH3L1_001882"
     CDS             complement(15000..15965)
                     /gene="trxB"
                     /locus_tag="ACH3L1_001882"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFT4076634.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16509..17003
                     /gene="lrp"
                     /locus_tag="ACH3L1_001883"
     CDS             16509..17003
                     /gene="lrp"
                     /locus_tag="ACH3L1_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFT4076635.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17138..21166
                     /gene="ftsK"
                     /locus_tag="ACH3L1_001884"
     CDS             17138..21166
                     /gene="ftsK"
                     /locus_tag="ACH3L1_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFT4076636.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21321..21932
                     /gene="lolA"
                     /locus_tag="ACH3L1_001885"
     CDS             21321..21932
                     /gene="lolA"
                     /locus_tag="ACH3L1_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFT4076637.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21943..23286
                     /gene="rarA"
                     /locus_tag="ACH3L1_001886"
                     /gene_synonym="mgsA"
     CDS             21943..23286
                     /gene="rarA"
                     /locus_tag="ACH3L1_001886"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFT4076638.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23377..24669
                     /gene="serS"
                     /locus_tag="ACH3L1_001887"
     CDS             23377..24669
                     /gene="serS"
                     /locus_tag="ACH3L1_001887"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFT4076639.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24909..27352
                     /gene="dmsA"
                     /locus_tag="ACH3L1_001888"
                     /pseudo
     CDS             24909..27352
                     /gene="dmsA"
                     /locus_tag="ACH3L1_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27363..27980
                     /gene="dmsB"
                     /locus_tag="ACH3L1_001889"
     CDS             27363..27980
                     /gene="dmsB"
                     /locus_tag="ACH3L1_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFT4076640.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27982..28773
                     /locus_tag="ACH3L1_001890"
     CDS             27982..28773
                     /locus_tag="ACH3L1_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFT4076641.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28809..29435)
                     /gene="ycaC"
                     /locus_tag="ACH3L1_001891"
     CDS             complement(28809..29435)
                     /gene="ycaC"
                     /locus_tag="ACH3L1_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFT4076642.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29750..30898
                     /locus_tag="ACH3L1_001892"
     CDS             29750..30898
                     /locus_tag="ACH3L1_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4076643.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30916..>31125
                     /locus_tag="ACH3L1_001893"
     CDS             30916..>31125
                     /locus_tag="ACH3L1_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFT4076644.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         7674 a         7629 c         8355 g         7467 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9721 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9781 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9841 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9901 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
     9961 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10021 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10081 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10141 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10201 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10261 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10321 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10381 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10441 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10501 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10561 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10621 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10681 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10741 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10801 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10861 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10921 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    10981 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11041 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11101 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11161 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11221 ggtaagccat taacaggcgt gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11281 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11341 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11401 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11461 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11521 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11581 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11641 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11701 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11761 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11821 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11881 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    11941 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12001 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12061 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12121 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12181 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12241 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12301 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12361 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12421 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12481 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12541 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12601 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12661 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12721 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12781 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12841 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12901 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    12961 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13021 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13081 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13141 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13201 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13261 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13321 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13381 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13441 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13501 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13561 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13621 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13681 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13741 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13801 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13861 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13921 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    13981 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14041 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14101 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14161 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14221 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14281 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14341 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14401 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14461 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14521 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14581 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14641 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14701 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14761 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14821 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14881 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    14941 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15001 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15061 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15121 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15181 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15241 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15301 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15361 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15421 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15481 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15541 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15601 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15661 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15721 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15781 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15841 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15901 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    15961 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16021 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16081 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16141 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16201 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16261 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16321 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16381 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16441 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16501 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16561 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16621 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16681 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16741 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16801 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16861 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16921 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    16981 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17041 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17101 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17161 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17221 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17281 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17341 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17401 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17461 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17521 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17581 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17641 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17701 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17761 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17821 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17881 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    17941 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18001 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18061 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18121 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18181 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18241 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18301 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18361 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18421 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18481 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18541 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18601 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18661 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18721 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18781 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18841 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18901 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    18961 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19021 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19081 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19141 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19201 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19261 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19321 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19381 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19441 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19501 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19561 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19621 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19681 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19741 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19801 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19861 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19921 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    19981 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20041 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20101 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20161 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20221 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20281 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20341 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20401 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20461 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20521 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20581 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20641 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20701 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20761 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20821 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20881 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    20941 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21001 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21061 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21121 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21181 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21241 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21301 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21361 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21421 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21481 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21541 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21601 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21661 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21721 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21781 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21841 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21901 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    21961 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22021 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22081 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22141 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22201 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22261 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22321 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22381 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22441 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22501 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22561 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22621 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22681 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22741 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22801 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22861 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22921 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    22981 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23041 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23101 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23161 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23221 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23281 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23341 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23401 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23461 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23521 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23581 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23641 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23701 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23761 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23821 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23881 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    23941 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24001 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24061 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24121 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24181 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24241 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24301 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24361 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24421 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24481 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24541 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24601 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24661 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24721 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24781 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24841 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24901 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    24961 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25021 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25081 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25141 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25201 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25261 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25321 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25381 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25441 ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg aactgctgcg gcggttatct
    25501 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25561 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25621 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25681 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25741 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25801 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25861 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25921 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    25981 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26041 accggcgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26101 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gagggaacag
    26161 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26221 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26281 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26341 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26401 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26461 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26521 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26581 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26641 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26701 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26761 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26821 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26881 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    26941 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27001 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27061 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27121 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27181 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27241 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27301 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27361 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27421 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27481 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27541 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27601 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27661 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27721 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27781 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27841 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27901 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    27961 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28021 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28081 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28141 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28201 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28261 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28321 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28381 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28441 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28501 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28561 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28621 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28681 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28741 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    28801 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28861 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28921 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    28981 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29041 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29101 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29161 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29221 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29281 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29341 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29401 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29461 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29521 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29581 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29641 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29701 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29761 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29821 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29881 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    29941 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30001 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30061 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30121 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30181 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30241 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30301 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30361 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30421 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30481 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30541 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30601 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30661 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30721 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30781 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30841 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30901 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    30961 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31021 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31081 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcag
//
LOCUS       DAZXUL010000042        30961 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZXUL010000042 DAZXUL010000000
VERSION     DAZXUL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30961
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.042"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..588
                     /locus_tag="ACH3L1_001894"
     CDS             <1..588
                     /locus_tag="ACH3L1_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFT4076645.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="ACH3L1_001895"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="ACH3L1_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFT4076646.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="ACH3L1_001896"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="ACH3L1_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFT4076647.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="ACH3L1_001897"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="ACH3L1_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFT4076648.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="ACH3L1_001898"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="ACH3L1_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFT4076649.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="ACH3L1_001899"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="ACH3L1_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFT4076650.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="ACH3L1_001900"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="ACH3L1_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFT4076651.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="ACH3L1_001901"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="ACH3L1_001901"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFT4076652.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="ACH3L1_001902"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="ACH3L1_001902"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFT4076653.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="ACH3L1_001903"
     CDS             complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="ACH3L1_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFT4076654.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="ACH3L1_001904"
     CDS             complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="ACH3L1_001904"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFT4076655.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="ACH3L1_001905"
     CDS             complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="ACH3L1_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFT4076656.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="ACH3L1_001906"
     CDS             complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="ACH3L1_001906"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFT4076657.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="ACH3L1_001907"
     CDS             complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="ACH3L1_001907"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFT4076658.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="ACH3L1_001908"
     CDS             complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="ACH3L1_001908"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFT4076659.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18720..18820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="ACH3L1_001909"
     CDS             complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="ACH3L1_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFT4076660.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19444..20217
                     /gene="nudC"
                     /locus_tag="ACH3L1_001910"
     CDS             19444..20217
                     /gene="nudC"
                     /locus_tag="ACH3L1_001910"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFT4076661.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20257..21321
                     /gene="hemE"
                     /locus_tag="ACH3L1_001911"
     CDS             20257..21321
                     /gene="hemE"
                     /locus_tag="ACH3L1_001911"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFT4076662.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21478..22149
                     /gene="nfi"
                     /locus_tag="ACH3L1_001912"
     CDS             21478..22149
                     /gene="nfi"
                     /locus_tag="ACH3L1_001912"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFT4076663.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22192..22782
                     /locus_tag="ACH3L1_001913"
     CDS             22192..22782
                     /locus_tag="ACH3L1_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFT4076664.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22969..23241
                     /gene="hupA"
                     /locus_tag="ACH3L1_001914"
     CDS             22969..23241
                     /gene="hupA"
                     /locus_tag="ACH3L1_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFT4076665.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23254..23949
                     /locus_tag="ACH3L1_001915"
     CDS             23254..23949
                     /locus_tag="ACH3L1_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFT4076666.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTRGYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="ACH3L1_001916"
     CDS             complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="ACH3L1_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFT4076667.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24614..25990
                     /gene="zraS"
                     /locus_tag="ACH3L1_001917"
     CDS             24614..25990
                     /gene="zraS"
                     /locus_tag="ACH3L1_001917"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFT4076668.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25987..27312
                     /gene="zraR"
                     /locus_tag="ACH3L1_001918"
     CDS             25987..27312
                     /gene="zraR"
                     /locus_tag="ACH3L1_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFT4076669.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27309..28598)
                     /gene="purD"
                     /locus_tag="ACH3L1_001919"
     CDS             complement(27309..28598)
                     /gene="purD"
                     /locus_tag="ACH3L1_001919"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFT4076670.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28610..30199)
                     /gene="purH"
                     /locus_tag="ACH3L1_001920"
     CDS             complement(28610..30199)
                     /gene="purH"
                     /locus_tag="ACH3L1_001920"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFT4076671.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            30814..>30961
                     /locus_tag="ACH3L1_001921"
     rRNA            30814..>30961
                     /locus_tag="ACH3L1_001921"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7278 a         8037 c         8518 g         7128 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggg cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggc
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cattgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggcagg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgtgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgt ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccaa gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgattg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaatatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacattgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggca tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcaaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag gggggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatattctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    11941 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacacct tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgtaaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13141 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13201 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13261 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13321 cataccggct gcaaaccctg agcggttaac acggcagcaa ccgcttccgg tctgcgatcg
    13381 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13441 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13501 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13561 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13621 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    13681 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    13741 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    13801 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    13861 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    13921 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    13981 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14041 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14101 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14161 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14221 cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc
    14281 atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt
    14341 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag
    14401 gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac
    14461 tgcggctgcc cggtccggac tgacgtgcca gcaggcctac ttctaccgcc agacgaaatg
    14521 ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt
    14581 cggcccccat ttccagcgcc tgcgtggcat ggctgggaac gccgatgcca gcatcgacaa
    14641 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    14701 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    14761 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    14821 gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    14881 gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    14941 tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga
    15001 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15061 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15121 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15181 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15241 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15301 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15361 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15421 caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc
    15481 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac
    15541 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag
    15601 gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc
    15661 gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg
    15721 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt
    15781 caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg
    15841 tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag
    15901 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    15961 gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16021 cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat
    16081 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16141 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16201 tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca
    16261 cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg
    16321 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16381 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16441 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16501 catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16561 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat
    16621 ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    16681 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    16741 acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga
    16801 catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct
    16861 gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga
    16921 ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    16981 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17041 cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag
    17101 cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca
    17161 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17221 atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17281 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc
    17341 ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg
    17401 caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17461 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17521 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17581 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    17641 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    17701 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    17761 tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    17821 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    17881 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    17941 caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18001 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18061 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18121 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    18181 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18241 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18301 gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc
    18361 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18421 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18481 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18541 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18601 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag
    18661 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    18721 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    18781 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    18841 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18901 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    18961 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19021 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19081 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19141 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19201 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19261 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19321 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19381 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19441 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19501 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19561 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag
    19621 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19681 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19741 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg tagccattgc
    19801 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19861 tcgctcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19921 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19981 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20041 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20101 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20161 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20221 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20281 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20341 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20401 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20461 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20521 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20581 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20641 cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20701 tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa
    20761 aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc
    20821 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    20881 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20941 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21001 accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc
    21061 ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21121 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21181 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21241 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21301 tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag
    21361 gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc
    21421 cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg
    21481 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat
    21541 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    21601 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    21661 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    21721 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    21781 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta
    21841 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    21901 ctctccagca aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    21961 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    22021 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    22081 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    22141 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    22201 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    22261 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    22321 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    22381 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    22441 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg
    22501 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    22561 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatggg cgatgaagag
    22621 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    22681 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    22741 gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    22801 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    22861 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    22921 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    22981 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    23041 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    23101 gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc gtactggccg caacccgcag
    23161 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    23221 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    23281 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    23341 gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    23401 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    23461 agcccgtggc gcagtggcga taccacgacg cgagggtatc gctggcaggg cgataacctc
    23521 acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac
    23581 gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    23641 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    23701 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    23761 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    23821 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    23881 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    23941 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    24001 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    24061 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    24121 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    24181 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    24241 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    24301 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    24361 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    24421 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    24481 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    24541 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    24601 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    24661 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    24721 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24781 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24841 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24901 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24961 ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    25021 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    25081 tggaatgtac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    25141 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    25201 tattctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    25261 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    25321 ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    25381 taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact
    25441 ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga
    25501 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    25561 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    25621 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    25681 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25741 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    25801 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    25861 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25921 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25981 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    26041 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    26101 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    26161 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    26221 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    26281 ggggcgcagg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    26341 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    26401 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    26461 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    26521 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    26581 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    26641 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    26701 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    26761 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    26821 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26881 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26941 atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    27001 aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt
    27061 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    27121 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat
    27181 attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    27241 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa
    27301 ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag
    27361 caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg
    27421 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca
    27481 tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg
    27541 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    27601 ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc
    27661 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27721 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27781 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27841 cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag
    27901 taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc
    27961 tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    28021 tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc
    28081 gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct
    28141 ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    28201 accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg
    28261 gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg
    28321 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    28381 tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg
    28441 atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta
    28501 cccggagcag caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    28561 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    28621 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    28681 cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg
    28741 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28801 ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28861 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca
    28921 cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac
    28981 gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca
    29041 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc
    29101 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    29161 aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat
    29221 ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    29281 cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct
    29341 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    29401 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg
    29461 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    29521 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    29581 ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt
    29641 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    29701 gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29761 cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat
    29821 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg
    29881 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29941 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    30001 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    30061 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    30121 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    30181 ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct
    30241 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    30301 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    30361 ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    30421 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30481 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgccttca tcgacacggc
    30541 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca
    30601 gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg cgctaaagcg
    30661 cgtcgcaact gctctttaac aatttatcag acaatctgtg tgggcactcg aagatacgga
    30721 ttcttaacgt cgcaagacga aaaatgaata ccaagtctca agagtgaaca cgtaattcat
    30781 tacgaagttt aattctttga gcatcaaact tttaaattga agagtttgat catggctcag
    30841 attgaacgct ggcggcaggc ctaacacatg caagtcgaac ggtaacagga agcagcttgc
    30901 tgtttcgctg acgagtggcg gacgggtgag taatgtctgg gaaactgcct gatggagggg
    30961 g
//
LOCUS       DAZXUL010000043        30300 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZXUL010000043 DAZXUL010000000
VERSION     DAZXUL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30300
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.043"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..817)
                     /gene="wcaD"
                     /locus_tag="ACH3L1_001922"
     CDS             complement(<1..817)
                     /gene="wcaD"
                     /locus_tag="ACH3L1_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HFT4076672.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(792..2009)
                     /gene="wcaC"
                     /locus_tag="ACH3L1_001923"
     CDS             complement(792..2009)
                     /gene="wcaC"
                     /locus_tag="ACH3L1_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFT4076673.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2006..2494)
                     /gene="wcaB"
                     /locus_tag="ACH3L1_001924"
     CDS             complement(2006..2494)
                     /gene="wcaB"
                     /locus_tag="ACH3L1_001924"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFT4076674.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2497..3336)
                     /gene="wcaA"
                     /locus_tag="ACH3L1_001925"
                     /pseudo
     CDS             complement(2497..3336)
                     /gene="wcaA"
                     /locus_tag="ACH3L1_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3429..5591)
                     /gene="wzc"
                     /locus_tag="ACH3L1_001926"
     CDS             complement(3429..5591)
                     /gene="wzc"
                     /locus_tag="ACH3L1_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFT4076675.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5594..6037)
                     /gene="wzb"
                     /locus_tag="ACH3L1_001927"
     CDS             complement(5594..6037)
                     /gene="wzb"
                     /locus_tag="ACH3L1_001927"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFT4076676.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6043..7182)
                     /gene="wza"
                     /locus_tag="ACH3L1_001928"
     CDS             complement(6043..7182)
                     /gene="wza"
                     /locus_tag="ACH3L1_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFT4076677.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7491..7640
                     /locus_tag="ACH3L1_001929"
     CDS             7491..7640
                     /locus_tag="ACH3L1_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076678.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7841..9424
                     /locus_tag="ACH3L1_001930"
     CDS             7841..9424
                     /locus_tag="ACH3L1_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFT4076679.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9876..11729)
                     /gene="asmA"
                     /locus_tag="ACH3L1_001931"
     CDS             complement(9876..11729)
                     /gene="asmA"
                     /locus_tag="ACH3L1_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFT4076680.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11751..12332)
                     /gene="dcd"
                     /locus_tag="ACH3L1_001932"
     CDS             complement(11751..12332)
                     /gene="dcd"
                     /locus_tag="ACH3L1_001932"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFT4076681.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12424..13065)
                     /gene="udk"
                     /locus_tag="ACH3L1_001933"
     CDS             complement(12424..13065)
                     /gene="udk"
                     /locus_tag="ACH3L1_001933"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFT4076682.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13383..13625
                     /locus_tag="ACH3L1_001934"
                     /pseudo
     CDS             13383..13625
                     /locus_tag="ACH3L1_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13624..14565
                     /gene="mdtA"
                     /locus_tag="ACH3L1_001935"
                     /pseudo
     CDS             13624..14565
                     /gene="mdtA"
                     /locus_tag="ACH3L1_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14565..17687
                     /gene="mdtB"
                     /locus_tag="ACH3L1_001936"
                     /pseudo
     CDS             14565..17687
                     /gene="mdtB"
                     /locus_tag="ACH3L1_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17688..20753
                     /gene="mdtC"
                     /locus_tag="ACH3L1_001937"
     CDS             17688..20753
                     /gene="mdtC"
                     /locus_tag="ACH3L1_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFT4076683.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20754..22169
                     /locus_tag="ACH3L1_001938"
     CDS             20754..22169
                     /locus_tag="ACH3L1_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4076684.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22166..23569
                     /gene="baeS"
                     /locus_tag="ACH3L1_001939"
     CDS             22166..23569
                     /gene="baeS"
                     /locus_tag="ACH3L1_001939"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFT4076685.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23566..24288
                     /gene="baeR"
                     /locus_tag="ACH3L1_001940"
     CDS             23566..24288
                     /gene="baeR"
                     /locus_tag="ACH3L1_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFT4076686.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24479..24811
                     /locus_tag="ACH3L1_001941"
     CDS             24479..24811
                     /locus_tag="ACH3L1_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFT4076687.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24958..26319
                     /gene="yegQ"
                     /locus_tag="ACH3L1_001942"
     CDS             24958..26319
                     /gene="yegQ"
                     /locus_tag="ACH3L1_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFT4076688.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26661..26978)
                     /gene="yegR"
                     /locus_tag="ACH3L1_001943"
     CDS             complement(26661..26978)
                     /gene="yegR"
                     /locus_tag="ACH3L1_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFT4076689.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27384..28283
                     /gene="yegS"
                     /locus_tag="ACH3L1_001944"
     CDS             27384..28283
                     /gene="yegS"
                     /locus_tag="ACH3L1_001944"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFT4076690.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28365..29138)
                     /locus_tag="ACH3L1_001945"
     CDS             complement(28365..29138)
                     /locus_tag="ACH3L1_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFT4076691.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29244..30284)
                     /gene="gatD"
                     /locus_tag="ACH3L1_001946"
     CDS             complement(29244..30284)
                     /gene="gatD"
                     /locus_tag="ACH3L1_001946"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFT4076692.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETANRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7012 a         8048 c         7828 g         7412 t
ORIGIN      
        1 tacctaaatc gcccagacgg gtggaaatat aaggaaacgc gattaccaca ccaacccgga
       61 atacagccag agaaatcaat gctaacggta attttttctt aatcgcctct ttattcagat
      121 attgaaatgc ccactccagc aaataaaaca ggataaaggt cataacccct gaaaacgatc
      181 cggataatat tatccctgcg agaatcaggg catctgtttt aggcgttttg ataccaaact
      241 gtttgatgct gagccaaatt gagattaatg ccagagcgaa aaatgccggt tcgaaataaa
      301 gtgctgtcgt gcgcttgccg ccgaatttaa tgaaattcag cacatagctg ttgctgtaaa
      361 tcagatattt cgaaatcgac tccataatac tgctgccacc ggtgaggata atttgcgcca
      421 tctccaccgc cgccagcgcc accaccagtc cgaccaccag ataaaagaaa cgtaatatct
      481 tacgatggtt gtgtggcgaa attgttttaa aacgaatact ccacaccatg ccaataatga
      541 tcacaatata gacaaacagc atagttgaag tgacgtattt accggcatcc aacgactgac
      601 caaacaggta gttaaacgcc gtgagtcccg cgccaatccc taaggcaatc atcaattttt
      661 taatgctgat gcgttctaaa aacagcagta acaagacagg taaaaaagtg acgatggtga
      721 tggggaaact ttcgccaagc tgggcgattt tgacgttaac cagcaggtag atcagtggca
      781 gcagcaggta gctacagatt ctgatagaag ttgacatact cctccagcat ctgttgtcca
      841 ctgtaggcgg cgcgactgcg ttggctgaac tcagccagcg tggtaccaaa tatcgcctgc
      901 gcgatttccg gtttgcttaa ctgtaccagt tgcagcacct cttcttcact gacggtttta
      961 ccaccggatt tttgcagcac ttcccgcgcc gcatcgctat gggtggcaat caccggcacg
     1021 ccaatcgata gcgcctcaca caaaatcagc gggtagttat cgacacgaga actgaacacc
     1081 agcgcatcca tctgattgag cgcgctcatc aacttgcgct tgtcagtttc aaagccgtga
     1141 ttaaccacgt tgccagcggt gaacggcgag aacttaccaa aggtatgcag ttcgattttg
     1201 tcgcccagcg ccatcatctc acgcaccagt tgctggttag ttttgccgtc gtaacgcagg
     1261 tcatgcgcca ccactgcgat tttcggcttg ccctgagttt cgcgcaccgg aggcagatct
     1321 gccagaatcg cttcggttgc catatcaatg ccattattga taatccggca acgccctggc
     1381 ccgtacagac tattgaaagc gtcagccaca tgctggctgg gggaaataaa ctgacagccc
     1441 agcgccagca tctcgcggaa taactggcgt ttgcccgcca ccagctggtg cgcgcgatca
     1501 atcttcaccg gcggatagtt atttaaggtc gggcatttct ggcagcccgt tttccagcct
     1561 tcgcaaccgt cggtaaaggc gcagcgtccg gttacactcc agtggtcgtg cagcgtccag
     1621 accagggtga catccggttt atggtttttc actttttcgc aaaagcgcac cacgctgtta
     1681 agattcaacc agtagctgtg cagcacatga aaatgcagga ccaccggacc cggtgtgcga
     1741 gtaatggtgc gatataactc attgaaattg ccgaacagat cgcgattaaa cagacgaaac
     1801 aacgcaatgt tcgccatcgc ggtcatccgc ggcgtatgtt tgatgacctg cggatagttc
     1861 tgatggctga cgctctcttt gccgcctttg ccgtagccgt agacaaaatg tgacgccagc
     1921 ccctgttgca gcgcacgctg gtggagatct aacgccacac ctgctgcccc gccttccgcc
     1981 agtcgcacat taaattgcag aatattcatt taatcacctt cactcgcgct ttttctccca
     2041 ccaccagcgc gttgtccggg acagagtcga gcaccacgct gcccgcaccc acggtgacgt
     2101 tgttaccaag cgtgatatca ccaagaataa tgacgttggc accgagttcg acgccgttgc
     2161 caatgtgtgg acatgccatg ttatcggcac cccgattgcc gatagtgacg ccgtggcgaa
     2221 tggtgaaatc atcccccgcc accacgtttt tattgatcac tacggcgtaa ccgtgatgga
     2281 tagtaaagcg gcggccaatg gtcgcggcgg cctggatttc ataaccgaaa aagcattcgg
     2341 tgataatgcg atacagcacc agcagcgggg ccgcccacag attgttgagg acgttctttt
     2401 tgcgccacac cgaacaaaaa tgagcgacgc gataggcaag taccatgcag cacgggcgta
     2461 aactccagct gttggcgcgc agatcttcca gcatctttag cgcccccgaa taccatcagc
     2521 cagacgtttg ccgttacgca ccgacagcag cgtcaacaag gtgcgccagg tcatacgttt
     2581 attgcggatc tggtagaggg taaacagctg atatttttta ctggcgcggt cgaatttgtc
     2641 tttgtgcttg cggtaaaagt ggaagtatcc ggagaatttt ttcggtgacg aggtgatctg
     2701 catttcgccg tgattgatat gcaggatctg cgtcgcctct tccactttcc acggttcgcc
     2761 gtactccacc accatccgca ggaatatgtc gtaatcctgt gctgctttca gttcggtatc
     2821 gaacaaacac tctttgaaac gccatgccca ggtaaagacc tgattgccaa tgatattacg
     2881 tttatagaac aggcggcgtg agtacggcga tttgggatac agtggtaagc tcgccggttg
     2941 ggaataaact tctccctagc aaacgtagtc gttagcgtac aaaaaggcgt gtgtgaccag
     3001 ttgctgttta tgggcgagga agacgctcag gcggttgggc gtccattcat catcatcatc
     3061 gatcccggtg atgtattccc cttgcgccag cataatcgcc tggttacgca ccgcgcacgc
     3121 cccgctgtta atgtcgttgt ggatgtaagt gatacgcgga tcgttgaggg cagtgacgta
     3181 ctgttgcagt tgttcccagg aggtggagca atcatccacg atgatcatct cccagttgct
     3241 gtagtcctgg cgcaggaccg attttatcgc ccgaatcgcc agttgttggc ggttccaggt
     3301 tggcatatag attgagatca gcggattgtc tttcataggt tgttctcccg gcttatgagc
     3361 tggtttgctt gccggatgcg gcgtgagcgc cttatccggc ctacggggcg gtgcgaatgc
     3421 aggcgtcgtt atttcgcatc cgacttatat tcgtattcgt aatagccata atcctgatac
     3481 gcgctggcgc ggcggaagat ggagttcaga atcacccctt tcaccggaat accgttttgc
     3541 tcaaagcggc tcagactggt ttccacttct ttcaatgtgt tgaccgcata acgcgccacc
     3601 attaacgtgg ttccgacatg acgaccaaca attgccgcat cggtcactgc cagaatcggc
     3661 ggcgtatcaa tcaacaccag gtcatagttt ttactcgccc agttcaccag ttcggcaaag
     3721 cgttcgctca ttaacagttc agaaggattt ggcggcacct gaccgcgcgg gatcaggtca
     3781 aatttggcaa tagaggtcgg tttagcagcg gtagtaatat cgccctgacc aatcagaatc
     3841 tccgacagcc cattgacatt attggtgccc aacagctcgt gggtgtagcc tttgcgcata
     3901 tcgcagtcga tcaacaacac gcgtttattg gtctggctga tcaccgccgc caggttggcg
     3961 cagacaaagg ttttaccgat tgacgggcta accccggtca tcatcaacac attgttctgc
     4021 gcctgcatca tcgcgaagtg cagactggta cgcagactac ggatggcttc aatcgccaga
     4081 tcggttggat tccccactgc cagtagctgg ctctgtttat agcgtttaac ccctttgatg
     4141 gttttgacgc tatcgcgcgc tttctgccac tccgacagcg ggatgctggc atagacgctg
     4201 attccgtgtt cttccagcac ttgcgggctt tcgatgccgc gattaaacaa cgagcgcagc
     4261 agcacaccca cgatagagag catcaggcca aggataatcg cgccgaggat aatcagccct
     4321 ttcttcggtt ttagcacgcc aggctgggtg attgccgggt caataatgcg cacatcgccg
     4381 acggtgctgg cttcggtgat tttcagctcc tgctctttat tcagcagttg catatagacc
     4441 tgctgaccag actcgacatc gcgggtcaga cggacaattt cctgctggtt tttcggcatc
     4501 gccgttacgc gaccgttaag tttggctttc tcttcttcca gcgcctgacg tttctccagc
     4561 agcgtgcggt acgccgggtg aactttggtg tacagcttgg agatttccgc ctctttaaag
     4621 gtcagttcgt tcaactgcgc gtcgatgttc accatcgaat cgagcaccgc tttcgcttca
     4681 agcggcagat caacagaatc tttatcctga cggaaggcat tcagtttgtt ttcggcaaca
     4741 tcgaggcggc tacgtacttc cggtaactgt tgcgcgagga aagcgaggct tttcgacgct
     4801 tccgccgatt tgcgctcaat attttgttcc tgatagttac gagcgatgct gttaagaatg
     4861 tcgcggatct gttcgcgatc ttcaccggta taagtcaggc tcagtacgcc tgcgtctttg
     4921 ccgttctccg ttaccgtcag gctgttttgc agttggttga tcatccccaa cgtggagtat
     4981 ttggtgacgg taaactcact gcccgggcgg gcgtgaatgg cttcaaccat cagcgtgacg
     5041 ccttcttttt tcagcatctg gcccgcttgc ccacgggcgc taaagccgcc atcactgctc
     5101 agggtgtagt ttttgtcatc cagcacatta agcgtaaaca cctgatccgc catctctttc
     5161 gggcggttaa aggtagtcac tttcaccgtc tcgttctgac gccccatcag gcgatcccag
     5221 cccgccccga aaatcgggaa cgtgtttttg ctcaccgcaa tatcgaggtc gagatcgtcc
     5281 accgttttac caagcaccag acgcgaacga atcaactgga tctcggcgtc cgatgcaggc
     5341 ggtttgttgg ctaatgccga gccgatatcc tgcaccagcg aattgccgct gttttgctcg
     5401 atttgtacca gtgcgtcggc actataaatc ggcgtggcga agaaagtgta aaccacggca
     5461 cagagggcaa atacgacggt gatgccaatc acccaccagc gcgcttcaat gacggtgccg
     5521 accaggcgac caatatcgat ttcatcactg cccgttaccg gagcggcatg ttgttttact
     5581 ttttctgtca ttcttatacc tgctctgcgt tcaatgcctg cgcccactgg cgggcagacc
     5641 gttcaagtaa tgtgtacacc gctgcaaacg tttcccggct tttgtgatac ggatcgggga
     5701 tttcacattc gttatcccag tgaccaaaca gcatcacttt gccgcgcatc tccggtgcca
     5761 tctcgcataa gcgttcgata tggcgcttat ccatggtcaa aatcaggtca tagttgcgac
     5821 acaggcggcg gctgatttgt cgtgcacagt ggccttccag agacagttga tgttctgcgg
     5881 cgacgctgat agcggtagga tcagcgccct taccgaccag cgcgccgagt ccagcggact
     5941 ccactttcag ttccgggtga taacgttgca gtaagcgttc cgccatcggg gaacggcaaa
     6001 tattgccgac acagacaact aagatgttgt taaacatgac gattaccagt tatgaatgtc
     6061 gctggctgta tccgtcatgt aacggacacc gctaatagtt ggcagcaact gattgatcag
     6121 acggttccag cgggaaaccg gggcggtggt gacatacacc acgtcataag gttgcaggcg
     6181 gaattctgtc gccatcacca gcgacgtcgc atcggacata tccagctggt agatattggc
     6241 aatcttgccg ttacgcccgc cctcgccttt cagcggacga atgacaaaga tgccgctggc
     6301 gttggaggtg gtcatgtcga taccttcggc attggccaga gcttcagtca gggtcatgcc
     6361 gctaaagtcc attttcaggg tgctctgttt cttcacttca cccatcacaa atactttcag
     6421 atcatcatta cgcggcacgt agagaatatc gccgggataa agcagtcggt tctgattgag
     6481 gtcgccgttt tgcatcagcg cctgtaaaga aatgcgttct tcacgaccat tgtgtgttag
     6541 caccacgttg cgccagtcag cggtgtcggt caggccacct gcggcgttga tggcgtcgag
     6601 aatggtcagt ggtacgttgg tgatcgcctg ttgaccggat ttattcacct gacctgagat
     6661 ataggccttt tgtgagcgga aggcggcgat attaacgtcc acctgcgggt cagcgatgta
     6721 cgtcgctaag cgcccggtaa tatcactgcg gatttcagcg agcgttttcc cgactacgtg
     6781 aaccttgccg atatacgggt aaaacatagt gccgtcaggc tgtacccagt tgccggtgtc
     6841 gctggagctg cggtactgac cagctggcgt ggtgagttcc gggtgatccc agacggtgac
     6901 attaagaacg tcccccggcc cgacgcgata ctggtaattc gcgatctcgc tttccagcgt
     6961 catattgggg cgcgctacat tcgggcgtgg gcgtaattgg tcaatcaggc gcggggtcag
     7021 cggataaaca ttcaccattt tgtcgagatc gaaatcagcg tcctgctgtt tgatgacgtc
     7081 tttgcccatc gtcgacatat tgctgcccgg aagtactgtg caaccgctta tcaaggttac
     7141 tgacaccaat aatggcatca atttcatttt ggatttcatc attgtttatt tatcactttg
     7201 gcagagtaat tatcctgtgc actattaata gcaatgtcgc catgcacatt taccttgcag
     7261 ttaattgaat aaaaatttaa ctggcatcag tcctaaaaaa attgatttca tccgcaggct
     7321 attgacagaa taattcagac tggtctttca ggcatccaga cacgctaccg cccctggctt
     7381 tttagctacc aatacactga ttgagcttaa tttttcaaac cctctctgca tacaatcgtt
     7441 gatgagaaag ggttattgcg gaaattaact tccgaatata aggtgacatt atggtaattg
     7501 aatattggct cgccaataat gcaggaggaa gtgttacagc taacggaata gcaggcaagg
     7561 taacaattcg gcaattggct atttttaaga attatattaa gtatcattca atatggtttt
     7621 taggagtttc tttaggttga caatatttaa tattgcgtct ccacatgcga tatttcttaa
     7681 ataatgtttt attattaccg cttttaattc agggataatg tgaggttatt accctcaaat
     7741 aatatgagat aaatagtgct gcaacattgc atttttgccc cgatttatcc atgatcgaat
     7801 tgtgacattt gtcatacaac gaataggttt tgtacttact atggaatgga ttgccgatcc
     7861 gtctatctgg gccgggttaa tcacgctgat tgtgatcgaa ctggtcctcg gcattgataa
     7921 cctggtcttt attgccatcc tcgccgaaaa actaccgccg aagcagcgcg accgcgcacg
     7981 ggttaccggg ctgctgctgg cgatgttaat gcgcctgtta ctgctagcgt caatctcctg
     8041 gctggtcacc ctgactcaac cgctgttcag cttccgctcg tttaccttta gcgcccgcga
     8101 cttaatcatg ctgtttggtg gtttcttcct gttgttcaaa gccacgatgg agctgaacga
     8161 acggctggaa gggaaagaca gcaataatcc cacacaacgc aaaggtgcga agttctgggg
     8221 cgtggtgacg caaattgtgg tactggacgc cattttctca ctcgactcag tgattaccgc
     8281 cgtcgggatg gtcgaccatt tactggtcat gatggccgcc gtggttatcg ctatcagcct
     8341 gatgttgatg gccagcaagc cgttaacgca atttgttaac agtcacccga ccatcgttat
     8401 tctctgctta agcttcctgt tgatgattgg ctttagcctg gtggcagaag gtttcggctt
     8461 cgtcattccg aaaggctacc tgtacgctgc cattggtttc tcggtgatga tcgaggcact
     8521 caatcagttg gctatcttta accggcgacg ttttctttcc gctaatcaga cgctacgcca
     8581 gcggactacc gaagcggtaa tgcgcctgct tagcgggcaa aaagaagatg cggaactgga
     8641 cgccaaaacc gcgtccatgc tggtggatca tggaaaccag cagatcttta atccgcagga
     8701 acggcggatg attgagcggg tacttaatct taaccagcgt accgtaagca gcattatgac
     8761 gtcgcgccac gatattgagc atatcgatct caacgcgccg gaagaagaaa tccgccagtt
     8821 gctggagcga aatcagcata cgcggctggt ggttaccgac ggtgatgacg cagaagattt
     8881 gctcggtgtt gttcacgtta tcgacctgct acaacagtca ctgcgcggcg aaccgctcaa
     8941 cctgcgggtg ttgattcgcc agccgctggt gttcccggaa accttgccgt tgttaccagc
     9001 cctggagcag ttccgtaatg cccgcacgca ctttgctttt gtggtggatg agtttggctc
     9061 ggtggaaggg attgtgacat taagtgacgt cactgaaacc attgccggta acttaccgaa
     9121 cgaagtggaa gagatcgacg cccgccatga tattcagaag aatgcggacg gttcctggac
     9181 ggcgaatggt catatgccgc tggaggatct ggtgcaatat gtgccgctgc cgctggatga
     9241 aaaacgtgaa tatcacacca ttgccgggct gttgatggag tatttgcagc gtattccaaa
     9301 acctggcgaa gaagttcagg tgggggatta tttgcttaaa acgttgcagg tggaaagcca
     9361 tcgcgtgcag aaggtgcaga ttataccgct gcgtaaggat ggcgagatgg agtacgaggt
     9421 gtgatggtat cggccatttt acccaatgct gcgtaacgcc ttataacttc agcaatgaag
     9481 cgttgtctgt ggtaaacgga catcacagcg ggggtgtggg tccggctgta aatcgccgaa
     9541 tcgtttcctg taggctgggg cagcccacat ggatgtgggc tgagggcgcg ttttacaggg
     9601 atgttacctc gcgcccgtac ccgatagccg aaaggaataa gatgagggaa ccgcgccagc
     9661 ggcgatttac cgccgggagc ccgggttgcc agggtggtgg cgattgagcc accctggcac
     9721 gttcaccggc ttgagcgttc catagtagcg aggaacatca agtgaacgga acagccttcg
     9781 atcttacata ttcagaatat aatagtgccg ccgcgaacgc ctgatgcgac gctttacgcg
     9841 tcttatcagg cctacaggac aggtcacctg gtaacctaca tcttctccag caacttcttc
     9901 acatctttgc cattgcggga atctttattc cgctcggccc agtcattcag gcgacgtttc
     9961 gcttcgtcct gtagatgttt gcgcagtaac tgatccactt gcaggctgta attgagttgc
    10021 tgccagttgc cataaacccg cagcggtact ggcgtttctt tgaggaaatc aatcagtttg
    10081 ctttccccgt tccagccacc cacgacccga atatcaaact gggtgtcgca ggtttgatct
    10141 gccagattca acatgccttc ccctgtcagc gccagcacag gcgattgacc ttgcatgtcg
    10201 tttaacgtca cgacgccatc cttcaacgtc aaatcggtgg taaagcggtc aagccgcgtc
    10261 acgttatcga agttttcagc ggccttcaca tcaccgccat tacgctctac cgcttgctga
    10321 atcatctgct ggaagttcat cccttccata cgtgtgtcgg tcatctcaac atgcgcctgt
    10381 ccttgccagt tgtggcggaa tgcgtcggca tctatgtcag caccggagaa atcaccagcc
    10441 agtgacattt ttccggtcaa cgaaatcgga tagttaaacg ccttcagaat ggtaccaatc
    10501 tcaacgttct ccagccgtgg ctggaagttt atccgcggat ttattgatgt cgcgtccagc
    10561 gtgcccggca gtgaaacctt tccaccgtta agcttgccct gcagttgagt aatttccagc
    10621 aaacccgact tgttggtcat ttgcgtggca acatctgtaa aattcattcc gcgccagcgc
    10681 acgttactgg cctgcaacaa aatatcagct gtaaagcctt gcagtccctg ataggccggt
    10741 tcatcaatac gcgaagaaat gaccgggcgc ggcaacgttg attggctctg cccctgctgc
    10801 gcggcaccgt tttcaccatt cgcggtttca ttaagcggga tgaggttgtc aagattcagt
    10861 tgcgggaatt gcagtctcag ctgccattcc ggtttctctg tcagcgtgac ctgtgcttgt
    10921 ccgctcagcg tactgtcatt ggcggtcaaa ctaatttggt taaatgaaag gcgtttatgt
    10981 gactcctgcc actgggactg gaaactcccc tgcccctgaa taccttgttt tggtaaatcg
    11041 gcaccctgca actgccagtt aatttgttca atagccgctg ttaaatcatg cggataatca
    11101 gaagcatcta ccgtaccgtt aagggatatc gtgagatcgc gctgatcgcg attaacccgc
    11161 ccggagaact caaatgagcc acgatgttgg ggatcttgtt ccatttgcag gcggatattg
    11221 cggattgtca cctgctcgtc atcttcatgc tggaacacca gaacgctgtc cgccacctta
    11281 agactggata tatcaaacga ccatccgcga tcgtctgaca gatccggcaa ggtattgtcg
    11341 cgcggtgcaa ccggagcgtc ttcactgcgc accgcttccg tctgcggcgt cagttggatc
    11401 actgcccctt ttagcatcac ctgcttaacg ctcagttgat gactcagtag tggtaaaagc
    11461 gccacgtcca gacgcatgtt gtcggcgcga accagtggct ggcttgcgcc ctgggcggtg
    11521 agagacattc gcccggagag gatactaagc tgcggccaga cgtgccaacg cagtggcccg
    11581 tcgagctgca attgataacc gctacgtgca gcaacttgct tgaccatata gtcgcggaaa
    11641 tcattcggat tcaccagcaa cactaacgca gataacccgg ccaccagcac gaccaagagt
    11701 atcatcagcg tcgtcagaaa tcgtctcatg gtatcctcaa tgggcctgaa ttagtcttta
    11761 tcgattcggc tggctaccgc gccctgctgg ttgcgatatt tcgcatcttc acggcggttg
    11821 taaggtcgcg ccgccgggcc ggaaagcggc tcaaagctca gcgcaccaat taacatgccc
    11881 ggacgcagcg ccagcggcag cttaccggag ttgtagaact ccagcacaat gcaaccagac
    11941 cagcccggat cgatacggtg cgcggtgacg tgcaccatca gccccagacg cgccaaagag
    12001 gaacgcccgt ccagccagcc caccagatcg gctggcagcg tcaccgactc cagtgtcacc
    12061 gccagcgcca gctctcctgg gtgaagatag aacgcctcgc cctcgtcgag aacgatctca
    12121 tcgctcatca cgcggtcaag cgcggcgctc acttcatctt tgggaccgct cagatcgata
    12181 aacgctgccg tgtgaccacg gaaggtacga aatttattgc ccaggcgtac atccaccgtc
    12241 gcgccgttaa tacgctccac tggcggacgt gggttgatcg acaaacggcc ttcatcaagc
    12301 caggcttcaa tatctcggtc acacagacgc atggcatttt ctcctttcgc acatcactcc
    12361 cttaacgcca attacgtcaa gggcatacca acttatcact gaacggtaca caatttatca
    12421 agcttattca aagaactgac ttattttcgc tttcaatata tcgatcgcga tgcggttttt
    12481 cccgccgcgc ggcacgataa tgtccgcata ttgtttagaa ggctcaatga attgcaggaa
    12541 catcgggcgc acggtttttt gatattgcgc catcacggaa tccattgaac gcccacgctc
    12601 gttaacgtca cgcttgatgc ggcgcatcag gcagatatcc agcggggtat caacgaaaat
    12661 ggagaagtta agttcgtcac gcaaacgcgc atccgtcagc aacaaaatgc cttcgagaat
    12721 gatgaccttc ttcggctcaa ccgtcaccgt ttctttcata cgcgtatgtt caacatagct
    12781 gtaaaccggc aggtcaattg ccgagccgcg tttcaacgct tgtaaatgct caagcaacag
    12841 gctgtgatcc atcgcgctgg gatggtcgta gttggtctta acgcgttctt ccatcgacag
    12901 atggctttga tctttgtaat agcagtcttc gggaattacg ccgatgtgtt catcaccgac
    12961 ttgctcacgt aattcacgat aaagggtact ggcaataaga ctcttgccgg aagccgatgc
    13021 gccagcgata ccgataatga cgcactgatg agactgatca gtcatatatt tagcgacctg
    13081 attaacctgg atgttaggaa gggggcgacg aagcgccaaa cgcggcaatt atagggattt
    13141 catccgtctg ataccagtcg aatagcgttg ccgcgcgctc agagttaatt gttgacaaag
    13201 aattcccggt ggcaaattac gttgatcagt tttatgtaag gtaaaaaatg ttatacgcag
    13261 ttgcgcaaat tatccgcctt tacgtcactt tatgagcaat tcgcatataa aatgtaaaac
    13321 ttttgtacta gcataaacac agaaacgaat actggcgacc tggccttgcg gataaagcgg
    13381 taataagcaa acaatcacag catgtattaa ttgccctgcc ccacccgctg cttcacctgg
    13441 tcagtttagg tttagtctcg tttatcttta cccttgtctc gcttgagctt tcgcagtttg
    13501 gcacccaact cgccccactg tggttcccga cgtccatcat gatggtggcg ttttatcgcc
    13561 atgccgggcg catgtggccg ggaattgcgc tgagctgttc gctgggaaat atcgccgcat
    13621 ccattccagg aaggccagca ggtcaaagca ggcgatttac tggcagaaat tgaccccagc
    13681 cagttcaaag ttgcattagc acaaacccag ggccaactgg caaaagataa agccacgctt
    13741 gctaacgccc gccgtgatct ggcgcgttat cagcaactgg caaaaaccaa tctcgtctcc
    13801 cgtcaggagc tggatgccca acaggcgctg gtcagtgaaa ccgaaggcac cattaaggct
    13861 gatgaagcaa gcgtcgccag cgcgcagttg caactcgact ggagccgcat caccgcacca
    13921 gtcgatggtc gcgttggtct caagcaagtt gatgttggta accaaatctc cagtggtgat
    13981 accaccggaa ttgtggtgat cacccagacg catcctatcg atttagtctt taccctgccg
    14041 gaaagcgata tcgctactgt agtgcaggcg caaaaagccg gaaaaccgct gatggtagaa
    14101 gcctgggatc gcaccaattc gaagaaatta agtgaaggca cgctgttaag cctcgataac
    14161 caaatcgatg ccacaaccgg tacgattaaa gtgaaagcac gctttaataa tcaggatgat
    14221 gcgctgtttc ccaatcagtt tgttaacgcg cgcatgttag tcgacaccga acaaaacgcc
    14281 gtagtgatcc caacagccgc cctgcaaatg ggcaatgaag gccattttgt ctgggtgctg
    14341 aatagcgaaa acaaggtcag caaacatctg gtgacgccgg gcattcagga cagtcagaaa
    14401 gtggtgatcc gtgcaggtat ttctgcgggc gatcgcgtgg tgacagacgg cattgatcgc
    14461 ctgaccgaag gggcgaaagt ggaagtggtg gaagcccaga gcgccaccac tccggaagag
    14521 aaagccacca gccgcgaata cgcgaaaaaa ggagcacgct cctgatgcag gtgttacccc
    14581 cgagcagcac aggcggcccg tcgcgcctgt ttattatgcg tcctgtcgcc accacgctgc
    14641 tgatggtggc gatcttactc gccgggatta tcggttatcg cgccctgccc gtttcggcgc
    14701 tgccggaagt ggactatccg accattcagg tggtcacgct ctacccaggt gccagcccgg
    14761 atgtcatgac ctctgccgtt accgcgccgc tcgaacgcca gttcgggcag atgtctggcc
    14821 tgaaacagat gtcgtcgcaa agttccggcg gtgcgtcagt tatcactttg cagttccagc
    14881 taacattacc gctcaatgtc gccgagcagg aagtgcaggc cgcgattaac gctgcgacca
    14941 acttgttgcc gagcgatctg cctaacccgc cggtttacag caaagtgaac ccggcagatc
    15001 cgccgatcat gacgctcgcc gtcacctcaa ccgccatgcc gatgacgcaa gtggaagata
    15061 tggtggaaac ccgcgtcgcg cagaaaatct cgcagatttc cggcgtcggc ctggtgacgc
    15121 tttccggcgg tcagcgtccg gctgttcgcg tcaaacttaa cgctcaggcg attgccgccc
    15181 tcggcctgac cagcgaaacc gtgcgcaccg ccattaccgg cgctaacgtt aactcggcaa
    15241 aaggtagcct cgacggccct tcccgtgcgg tcacgctttc cgcgaacgac cagatgcaat
    15301 ccgccgaaga gtatcgccag ctaatcatcg cctaccagaa cggcgcgcca attcgtctgg
    15361 gcgatgtcgc aactgtagag caaggtgcag aaaacagctg gctcggcgcg tgggcgaaca
    15421 aagaacaggc cattgtgatg aatgttcagc gccagcccgg tgctaacatt atctccaccg
    15481 ccgacagcat tcggcagatg ctgccacagc tcactgagag tctgccgaaa tcggtgaagg
    15541 tgacagtgct ttccgatcgc accaccaata tccgcgcatc cgtcgatgat actcagtttg
    15601 aattgatgat ggctatcgcg ctggtagtca tgattatcta cctgtttttg cgcaatattc
    15661 cggcgaccat cattcccggt gttgctgtac cgctgtcgtt aatcggcact ttcgcggtta
    15721 tggtgtttct cgatttttca atcaataacc tgacactgat gactttaact atcgccaccg
    15781 gattcgtggt cgatgacgcc atcgtggtga tcgaaaacat ttcccgctat atcgaaaaag
    15841 gcgaaaaacc gttggcggcg gcgctcaagg gcgcaggtga aatcggcttt accattatct
    15901 cgctgacctt ctcactgatt gcggtgttga tcccactgct gtttatgggc gatatcgtcg
    15961 ggcgactgtt ccgcgaattt gctattaccc tggcggtagc gattttgatc tcagcggtgg
    16021 tgtcgctgac cctgacaccg atgatgtgcg cgcggatgct cagccaggag tcgttgcgta
    16081 aacagaacag cttctcccgt gcctcggaaa aaatgttcga caggataatc gccgcctatg
    16141 gtcgtggact ggcgaaagtg ctgaatcatc cgtggctgac cttaagcgtg gcactcagca
    16201 cgctgctgct tagcgtgctg ctgtgggtgt tcattccgaa aggtttcttc ccggtacagg
    16261 acaacggcat tattcagggc actttgcagg caccgcaatc cagctccttt gccaatatgg
    16321 cccagcgaca acgccaggtc gcggacgtga ttttgcagga tccggcagtg caaagcctga
    16381 cctcatttgt tggcgttgat ggcactaacc cgtcgctgaa tagtgcacgt ttacaaatca
    16441 acctcaaacc gttggatgaa cgtgatgatc gggtgcaaaa agtcatcgcc cgtctgcaaa
    16501 cggcggtaga taaagtgccg ggcgtcgatc tcttcctgca accaacgcag gacctgacca
    16561 tcgatactca ggtcagccgc acccagtacc agtttacctt gcaggccacg tcactggatg
    16621 cgctcagtac ctgggtgcca cagttgatgg aaaaactcca gcaactgcca cagctttctg
    16681 atgtctccag cgactggcag gacaaagggc tggtggcgta tgtcaatgtt gatcgcgaca
    16741 gcgccagccg tctggggatc agcatggcgg acgtcgataa cgccctgtac aacgcgtttg
    16801 gtcagcgact gatttccact atttatactc aggctaacca gtatcgcgtg gtgctggagc
    16861 acaataccga aaatacccca ggcctcgcgg cgctggatac cattcgcctg accagcagcg
    16921 acggtggcgt ggtgccgtta agctcaattg ccaaaattga gcagcgtttt gcgccgcttt
    16981 ccatcaacca tctggatcag ttcccggtaa caaccatctc ctttaacgtg ccggataact
    17041 attcgctggg cgatgcggtg caggcgatta tggacaccga aaagacgctg aatctgccgg
    17101 tggatatcac cacgcaattc tagggcagca ccctcgcctt ccagtcggcg ctgggcagca
    17161 ctgtctggct gattgtcgcg gcggtggtgg cgatgtatat cgtgctcggc attctgtacg
    17221 agagctttat tcacccgatc accattctct cgacgctacc caccgcaggg gtcggcgcgc
    17281 tgctggcatt aatgattgcc ggtagcgaac tggatgtgat tgcgattatc ggcattattt
    17341 tgctgatcgg tattgtgaag aagaacgcca tcatgatgat cgacttcgcg ctggctgctg
    17401 agcgcgagca aggcatgtcg ccgcgcgatg caatctacca ggcttgtctg ttgcgttttc
    17461 gtccgatcct gatgaccacc ctggcggctc tgcttggcgc gctgccgctg atgttgagta
    17521 ccggggtcgg cgcggaactg cgtcgtccgt taggtatcgg catggtcggc ggtctgattg
    17581 tcagccaggt gctgacgctg ttcaccacgc cggtgattta tctgctgttc gaccgcctgg
    17641 cattgtggac caaaagtcgc tttgctcgcc atgaagagga ggcgtaagtg aagttttttg
    17701 ccctcttcat ttaccgcccg gtggcgacga ttttactgtc ggtcgccatt accctgtgcg
    17761 gcatactggg tttccgtatg ctgccggtcg ccccgctgcc gcaggtcgat tttccggtga
    17821 ttatggtcag cgcctcgctg cccggtgcgt cgccggagac aatggcgtct tccgttgcca
    17881 cgccgctgga gcgctcactt gggcgcattg ccggggttag tgaaatgacc tccagcagtt
    17941 cgctcggcag cacgcgcatt attttgcagt ttgattttga ccgggatatc aacggcgcag
    18001 cgcgtgatgt gcaggcggcg atcaacgctg cacaaagttt gctgcccagc gggatgccca
    18061 gccgcccgac ctatcgcaaa gcgaacccgt cggatgcgcc aattatgatc ctcacgctga
    18121 catccgatac ttattcgcag ggtgaactgt acgatttcgc ctcgacgcag ctggctccga
    18181 cgatttcgca aatcgacggt gtcggtgatg tcgatgtcgg tggcagctcg ctgcccgccg
    18241 tgcgcgtcgg gctgaccccg caggcgctgt ttaatcaggg cgtgtcgctg gacgacgtac
    18301 gcactgccat cagcaatgcc aacgtgcgta aaccgcaggg ggcgctggaa gatggcactc
    18361 accgctggca gatccagacc aacgatgaac tgaaaaccgc cgctgaatat cagccgctga
    18421 ttattcacta caacaacggc ggcgcggttc gtctgggcga tgtggcgacg gtgactgact
    18481 cagtgcagga tgtgcgcaac gccgggatga ccaacgccaa accggctatt ttactgatga
    18541 tccgcaaact gccggaagcc aatattatcc agacggtaga cagcatccgg gcaaaattac
    18601 cagagttgca ggagaccatt ccggcggcga ttgatctgca aattgcccag gatcgctccc
    18661 ccaccattcg cgcctcgctg gaagaagtcg agcaaacgct gattatctcg gtggcgctgg
    18721 tgattctggt ggtcttttta ttcctgcgct cgggtcgcgc cactattatt cccgccgttg
    18781 cggtgccggt ttcgctgatt ggtacgtttg cggcgatgta cctgtgcgga ttcagtctca
    18841 ataatctttc gctgatggcg ctcaccattg ctactggttt cgtggtggat gacgccattg
    18901 tggtgctgga aaacattgcc cgtcatctgg aagcagggat gaaaccacta caggccgcgc
    18961 tgcaaggtac tcgcgaagtc ggctttacgg tgctgtcgat gagtctgtca ctggtggcgg
    19021 tgttcctgcc gctgctgttg atgggcggat tgccgggccg actgttacgc gagtttgccg
    19081 tgacgctttc tgtcgccatt ggtatttcgt tgctggtttc cctgacgcta acgccaatga
    19141 tgtgcggctg gatgctaaaa gccagcaagc cgcgtgagca aaagcgactg cgtggttttg
    19201 gccgcatgtt ggtagccctg caacaaggct acggcaagtc gctgaaatgg gtgctcaatc
    19261 atacccgtct ggtgggcgtg gtgctgcttg gcaccattgc gctgaatatc tcgatcccga
    19321 aaaccttctt cccggagcag gacaccggcg tactgatggg tgggatacag gcggatcaga
    19381 gtatttcgtt tcaggcgatg cgcggcaaat tgcaggattt catgaaaatt atccgtgacg
    19441 atccggcagt ggataatgtc accggcttta ccggcggttc acgagttaac agtgggatga
    19501 tgtttatcac cctcaagcca cgcgacgaac gcagcgaaac ggcgcagcaa attatcgacc
    19561 gtctgcgcgt gaagctggcg aaagaaccgg gggcgaatct gttcctgatg gcggtacaag
    19621 atattcgcgt tggtgggcgt cagtcgaacg ccagctacca gtacacgttg ttatccgacg
    19681 acctggcggc actgcgcgaa tgggagccga aaatccgcaa aaaactggcg acgttgccgg
    19741 aactggcgga cgtgaactcc gatcagcagg ataacggcgc ggagatgaat ctggtttacg
    19801 accgcgacac catggcacgg ctgggaatcg acgtacaggc cgccaacagt ctgttaaata
    19861 acgccttcgg tcagcggcaa atctcgacca tttaccagcc gatgaaccag tataaagtgg
    19921 tgatggaagt ggatccgcgc tatacccagg acatcagtgc gctggaaaaa atgttcgtta
    19981 tcaataacga aggcaaagcg atcccgctgt cgtatttcgc taaatggcaa ccggcgaatg
    20041 ccccactatc ggtgaatcat cagggattat cggcggcctc gaccatctcg ttcaacctgc
    20101 cgaccggaaa atcgctctcg gacgccagtg cggcgatcga tcgcgcaatg acccagcttg
    20161 gtgtgccttc gacggtgcgc ggcagttttg ccggcacggc gcaggttttc caggagacga
    20221 tgaactcgca ggtgatcctg attatcgccg ccatcgccac ggtgtatatc gtgctgggta
    20281 tcctttacga gagttacgta catccgctga cgattctctc caccctgccc tcggcgggcg
    20341 ttggagcgct gttggcgctg gagctgttca atgccccgtt cagcctaatc gccctgatag
    20401 ggatcatgct attaatcggc atcgtgaaga aaaacgccat tatgatggtc gattttgcgc
    20461 ttgaagccca acggcacggt aacctgacgc cgcaggaagc tattttccag gcctgtctgc
    20521 tgcgttttcg cccgattatg atgaccaccc tggcggcgct gtttggcgcg ctgccgctgg
    20581 tattgtcggg cggtgacggc tcggagctgc ggcaacccct ggagatcacc attgtcggcg
    20641 gtctggtgat gagccagctc ctgacgctgt ataccacgcc ggtggtgtat ctctttttcg
    20701 accgtctgcg gctgcgtttt tcgcgtaaac ctaaacaaac ggtaaccgag taaatgacag
    20761 atcttcccga cagcacccgc tggcaattat ggattgtggc tttcggcttc tttatgcagt
    20821 cgctggacac caccatcgta aacaccgccc ttccctcaat ggcgcaaagc ctcggggaaa
    20881 gtccgttgca tatgcacatg gtcattgtct cttatgtgct gaccgtggcg gtgatgctgc
    20941 ccgccagcgg ctggctggcg gacaaagtcg gcgtgcgcaa tattttcttt accgccatcg
    21001 tgctgtttac tctcggttca ctgttttgcg cgctttccgg cacgctgaac gaactgttgc
    21061 tggcacgcgc gttacagggc gttggcggtg cgatgatggt gccggtcggc aggttgacgg
    21121 tgatgaaaat cgtaccgcgc gagcaatata tggcggcgat gacctttgtc acgttacccg
    21181 gtcaggtcgg tccgctgctc ggtccggcgc tcggcggtct gctggtagaa tacgcgtcgt
    21241 ggcactggat ctttttgatc aacattccgg tggggattat cggtgcgatc gccacattga
    21301 tgttaatgcc gaactacacc atgcagacgc ggcgctttga tctttccggc tttttattgc
    21361 tggcggttgg catggcggta ttaaccctgg cgctggacgg cagtaaaggt acaggtttat
    21421 cgccgctggc gattgctggc ctggtcgcag ttggcgtggt ggcactggtg ctttatctgc
    21481 tgcacgccag aaataacaac cgcgccctgt tcagtctgaa actgttccgc actcgtacct
    21541 tttcgctggg cctggcgggg agctttgccg gacgtattgg cagtggcatg ttgcccttta
    21601 tgacaccggt tttcctgcaa attggcctcg gtttctcgcc gtttcatgcc ggactgatga
    21661 tgatcccgat ggtgcttggc agcatgggaa tgaagcgaat tgtggtacag gtggtgaatc
    21721 gctttggtta tcgtcgggta ctggtagcga ccacgctggg tctgtcgctg gtcaccctgt
    21781 tgtttatgac taccgctctg ttgggctggt actacgtttt gccgttcgtc ctgtttttac
    21841 aagggatggt caactcgacc cgtttctcct ccatgaacac cctgacgctg aaagatctcc
    21901 cggacaatct ggcgagcagc ggaaacagcc tgctgtcgat gattatgcaa ttgtcgatga
    21961 gtatcggcgt caccatcgcc gggctgttgc tggggctttt tggttcgcag catatcagcg
    22021 tcgacagcgg caccacacaa accgtcttta tgtacacctg gcttagcatg gcgtttatca
    22081 tcgcccttcc ggcgttcatc tttgccagag tgccgaacga tacgcatcaa aatgtagcta
    22141 tttcgcggcg aaaaaggagc gcgcaatgaa gttctggcga cccggtatta ccggcaaact
    22201 gtttctggcg attttcgcca cctgcattgt cttgctgatc agtatgcact gggcggtgcg
    22261 tatcagtttt gagcgtggct ttattgatta catcaaacac ggcaatgaac agcgattaca
    22321 gctgttaagt gatgcgcttg gcgagcagta tgcgcagcat ggtaactggc gcttcctgcg
    22381 caacaacgat cgctttgtct ttcagatcct gcgttcattt gaacacgata attcggaaga
    22441 taaacccggc ccgggtatgc cgccgcacgg ctggcgtacc cagttctggg tggtcgatca
    22501 aaacaacaaa gtactggttg gtccgcgagc gccgattcca cctgacggta cacggcgacc
    22561 cattctggtc aacggtgcgg aagtgggcgc ggtgattgcc tcccctgttg agcggctgac
    22621 ccgcaatact gatatcaatt tcgataaaca acagcggcaa accagctggc tgatcgtcgc
    22681 cctggcaacg ttactcgcgg cgctcgccac ctttctgctg gcgcgcggtt tgctggcacc
    22741 ggtaaaacga cttgtcgatg gcacgcacaa actggcggcg ggcgatttca ctacccgcgt
    22801 aacgcccacc agtgaagatg aactgggcaa actggcgcaa gacttcaacc agctcgccag
    22861 cacgctggag aaaaaccagc aaatgcggcg cgattttatg gccgatattt ctcacgaact
    22921 gcgtacgcca ttagcggtgc tgcgcggtga actggaagcc attcaggatg gcgtgcgcaa
    22981 attcacgccg gagacggtgg cgtctttaca ggcggaggtc ggtacactga ccaaactggt
    23041 tgacgatctt catcagttgt cgatgtctga tgaaggcgct ctcgcctatc aaaaagcacc
    23101 ggtagatttg atcccactgc tggaagtggc gggcggcgca tttcgcgaac gattcgccag
    23161 ccgtggcctg aaactgcaat tttccctgcc agacagtatc accgtatttg gcgatcgcga
    23221 ccgtttaatg cagttattca ataacttact ggaaaacagc ctgcgctaca ctgacagcgg
    23281 cggcagcctg aaaatctctg ccgagcagca cgacaaaacg gtgcgcctga cctttgccga
    23341 cagtgcgcca ggtgtcagtg acgatcagct acaaaaattg tttgaacgtt tttatcgcac
    23401 cgaaggttcc cgcaaccgcg ccagcggcgg ttccgggctg gggctggcga tttgcctgaa
    23461 cattgttgaa gcccataatg gccgcattat tgccgcccat tcgccttttg gcggggtaag
    23521 cattacagta gagttaccgc tggaacggga tttacagaga gaagtatgac cgagttacca
    23581 atcgacgaaa acacaccgcg tattttgatc gtggaagatg aaccgaagct ggggcagttg
    23641 ctcattgatt atctgcgtgc tgcgagctat gcgccgacgc ttatcagcca cggcgatcag
    23701 gtactggcgt atgtgcgcca gacaccaccg gatctgatcc tgttggatct gatgctccct
    23761 ggcaccgatg gcctgacatt atgccgggaa attcgtcgtt tttccgatgt tccgatcgtg
    23821 atggtgacgg caaaaatcga agagatcgac cgcctgctgg ggctggagat tggcgccgat
    23881 gactacatct gcaagccgta cagcccacgg gaagtggtag cgcgtgtcaa aaccattttg
    23941 cgccgttgta aaccgcaacg cgaacttcag caacaggatg ccgaaagccc gttgattatc
    24001 gacgaaggtc gttttcaggc ttcatggcgc ggtaaaatgc ttgacctgac gcctgcggaa
    24061 tttcgtctgc ttaaaacgct ctctcacgaa ccaggaaaag tgttctcccg cgagcaattg
    24121 ctcaatcatc tttatgacga ctaccgcgta gtaaccgacc gcaccattga cagccacatt
    24181 aaaaatctgc gccgcaagct ggaatctctc gacgccgaac agtcatttat ccgcgccgtt
    24241 tacggcgttg gttaccgctg ggaagccgac gcctgccgca tcgtttagtt ttagcgacat
    24301 tactttgtta gccggagatg gcgttcagac ccgatccggc aacattattc cacgcatggt
    24361 cagcaaactg acattatctc tccgccctgg caggcatcag aaaatcgctc atactttaat
    24421 cagtaaacag cacctttaga tgctgttttc gataagcaat ttcaatcaag gagtcattat
    24481 ggctggttgg tttgaactca gtaagagcag tgataatcag ttccggtttg tgctaaaagc
    24541 gggcaatggt gagactatcc tcaccagcga gctgtatacc tcaaaagcct ctgcggaaaa
    24601 gggcatcgcg tcggtgcgta gcaacagccc gcaagaagag cgctatgaga aaaaaacggc
    24661 aagcaacggc aaattctatt tcaatctgaa agccgctaat catcaaatta tcggctccag
    24721 ccagatgtac gccaccgcgc aatctcgtga aaccggaatt gcctccgtta aagccaatgg
    24781 cacaagccag acggtgaaag acaatacgta attacgatgc cgggcgtggg ttgttggcgt
    24841 ccggcgcttt accagatccc ccctacagcg ctacaatgcc cgcccttaaa gtgggggcct
    24901 cccctaaccg cttcatcagg tgaagcggat ctgacctgtc atcagaacga gagaattatg
    24961 tttaaaccgg aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct
    25021 tatggcgcag atgctgttta tgcgggccag ccgcgttact ccctacgtgt gcgcaacaac
    25081 gaattcaacc acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag
    25141 ttttatgtcg tggtcaacat tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac
    25201 ctgaaaccgg tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt
    25261 atgctggtgc gtgagcactt ccctgaaatg ccgatccacc tctcggtgca ggctaacgcc
    25321 gtgaactggg cgacggtgaa attctggcag caaatgggac tgacccgcgt gatcctctct
    25381 cgcgagctgt cgctggaaga gattgaagag atccataatc aggtgccgga tatggagatc
    25441 gagatcttcg ttcacggcgc actgtgcatg gcctactccg gtcgctgcct gctctctggc
    25501 tatatcaaca agcgcgaccc gaaccagggc acctgcacca acgcctgccg ctgggagtac
    25561 aacgtccagg aagggaaaga agatgacgtt ggcaacatcg tacacaagta cgagccgatt
    25621 ccggtgcaaa atgttgagcc gacgctgggt atcggcgcgc caaccgacaa agtgtttatg
    25681 atcgaagaag cccagcgtcc gggcgagtat atgaccgcgt ttgaagatga gcacggcact
    25741 tacatcatga actcgaaaga tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg
    25801 ggcgtgcatt cgctgaaaat cgaaggtcgt accaaatctt tctactattg cgcacgcacc
    25861 gcacaggttt accgcaaagc tatcgatgac gccgctgcgg gaaaaccgtt cgataccagc
    25921 ctgctggaaa ctctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt
    25981 catactcacg acgattatca gaactacgaa tacggttatt cagtttctga ccgccagcag
    26041 tttgttggtg agtttaccgg tgagcgcaag ggggacctcg cagcggtagc ggtgaaaaat
    26101 aaattctccg ttggcgacag tcttgagctg atgacgccgc aaggcaacat taactttacc
    26161 cttgagcaca tggaaaacgc caaaggcgaa gctatgccgg tcgcaccagg cgatggttat
    26221 actgtgtggc tcccggtccc gcaggatctt gagctaaatt acgcgctgct aatgcgtaat
    26281 ttctccgggg aaaccacgcg taacccccac ggtaagtgat taatttcgat tatttttccc
    26341 ggatggaaaa ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt
    26401 ttcgctgaaa aacataatcc ataaaatgct agctgtacca ggaaccacct ccttagcctg
    26461 tgtaatctcc cttacacggg cttatttttt gtacataagc tgtaaagaag gattgatccc
    26521 tgataaccgt cttccattga ccacatcgat agaatccccc ttcatagcac aatgcctttc
    26581 acgtaacggt atcgtgctcg cccaggttcc ggctacgcac agccagaacg cgcatgtttg
    26641 acgcttacaa aaaaatattc tcactctcca catttgaatg tcagacgagc gacgccatgt
    26701 aatcctgcac cttctgtctt caggtcaact atctgcattt ttttgccctg agtaacacag
    26761 aaatgggctg catcattttt tactatattt tctgcaccag atattctgcc cctggctaaa
    26821 gaagcttcgg cttcggtgta gtattggtta tcgagtttac gctgaatatt acttttatat
    26881 gcaagaccaa atttaccgat acttgtttca tcattatgca cagcacaacc agatataaga
    26941 aaaatactaa ttaatgatat agcagctatc tttttcatct caccttcccc cattaaatac
    27001 caacgacact ctctagtgtt taaatataat aatggcatga ttattataat tgaataggat
    27061 tataataaat gttctgtaca acatttccta cataagtagg aattacggac attgaggccc
    27121 ttcagggtaa ctccatgggg gctttaatat attatattga agatgccact gtttagttga
    27181 atattaggta tatgctcttt tttgaaattt atcggtggca gccgttagta ttcgctgtcc
    27241 ccattgcaag ctcctggtgg taaccactga atcctccata cttgaactga ctttttatcc
    27301 tccgactttc atcctgttct gactccacct tttgttttct gctctacact atctacagac
    27361 caatcataaa ggcacatacg atcatggcag aatttcccgc cagcttactg attcttaatg
    27421 gtaaaagtac tgacaatcta cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa
    27481 tgacgatcca tgtgcgggtc acctgggaga aaggtgatgc cgcacgattt gtagaggagg
    27541 cccggaagtt gggcgtcgca acggtgattg ccggtggtgg cgatggcacc attaatgaag
    27601 tttctacggc gttgattcag tgtgaggggg atgacatacc cgcgctggga attttgccat
    27661 taggaaccgc caatgatttt gccaccagtg tagggattcc tgaggcactg gataaggcgc
    27721 tgaaactggc aattgccggt aatgccattg cgatagatat ggcgcaggtc aacaaacaaa
    27781 cctgttttat taatatggcg acaggcggat ttgggacgcg tattaccaca gaaacgccgg
    27841 aaaaattaaa agccgcgctg ggtggcgtct cttacatcat tcatggctta atgcgcatgg
    27901 atactctgca accggaccgt tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg
    27961 ccctggtcat tggtattggt aacgggcgtc aggccggtgg cggtcaacaa ttgtgcccga
    28021 acgcgttaat taacgatggc ttgctgcaac tgcgcatttt taccggcgat gaaattcttc
    28081 cggctctcgt atcaacctta aaatctgacg aagataaccc gaatattatc gaaggcgctt
    28141 cgtcgtggtt tgatatacaa gccccacacg aaatcacttt taatcttgat ggcgaaccgt
    28201 tgagtgggca aaacttccat attgaaatac ttccggcggc gttgcgttgt cgattaccac
    28261 cagattgtcc attattgcgt taatcaaact attctctctt gaaccaggga gaatccgtaa
    28321 tgccggatgc ggtgtaaaca ccttatccgg catacagaac aatactacac cgtaattaat
    28381 tttacccgtg tcttctgcaa tgccaattta tccacctctg acacagcatc atcggtaaca
    28441 ataatgtcaa ttttttcgat tggcagtacc tgattaaaac cgcgacgatt aaatttcgat
    28501 gaatcgagaa cggcaatcac tttatttgcg gcggtgacca tcgccccgct aatcgaataa
    28561 ccttcattaa aggtcgtaat accattaacc gcatcaatac catcagcacc gacaaacatt
    28621 aaatcggcat taatatcctg caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt
    28681 ttatggcgga ccgtgccacc acagacgacc agagtaatgt ctttattttc ggaaagggca
    28741 aacgctgccg ggagactgtt ggtaatcacc gtgatatttt tagcggtcat taatccttcg
    28801 gcgataagca ttgtggtact tccgctgtcg agaataacgg tcatcccttc atggatcatt
    28861 ttgactgccg cctgcgctat tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta
    28921 aacccgactt cctgggtctc ggtttcacta ttaccggaca ttattttcgc cgcaccgcca
    28981 tgaaagcgcg taaccacgcc tttttgttcg agaaagcgca aatcggcacg gattgtcgct
    29041 tccgaggcag caaatactcc cgccagatcc tgaacaagca cggttccctg ttcattcact
    29101 aattggatga tcttattcct tcgctcgaat gaattcatcg tcatttaccc atattcaatt
    29161 gtggctagtg taaacgaagg gggaccattg gtgaatgatc tgttttgcac aataccgggc
    29221 cagcatcacg ctggcccgcg gtttcatgga atgagcaaca ctttgccctg catggcatta
    29281 cgagcgatgt cacgcaccgc ctgggtgaag ctttcaaagc ttccacggtg agcgattaat
    29341 ggctccaggc ttaacttacg ttctgtcagc aaccggttcg ccgtttccca ctcctgcccc
    29401 ggccaagggc tggaatagtt catccaactg ccgataaccg tcagctcttt acgcaatatt
    29461 ttgccaaacg ttgccgatgt taaatgcaga tcctgatgca acgtgcccac cagcgccagt
    29521 tgggcatgag gaccggcaat ctctaccgcc agttcgacag tttgcggtac gccagccgtc
    29581 tcgaggataa gctgattaaa gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc
    29641 atttcgctac tgttaaatgt ttgcatcgca ccgaaagatt ttgccagtgc cagtttttct
    29701 gagctaatgt cgatcgccgt cacactcttt gctcccagcg cgacagcgca ctgaatagcc
    29761 agcaggccaa tggttccggc accaataata ataacgtttt tattctcaca accttgcgct
    29821 aaatgaaaag catgcaggcc aacggtaatc ggctcaataa aagccccatc ctcaataggc
    29881 atatccgtgg gtagagcaaa gacatttttt cgcttaacga caatatattc agcaaatcca
    29941 ccatcacgcc gcgagccaat aaaatcatat tttgcgcact gggaataaaa ccctttcaaa
    30001 cactctggac aagtaaaaca gggtaataac ggcacacagg caaccgcatc gccagggtgt
    30061 aaatcatcaa caccggatcc caccgcatca atatagccgc taaattcatg gcctaacgtt
    30121 attggataat aatgtgcacc atttttaaat atcctgggta aatcggaacc acataagccc
    30181 gagctggcaa tttttacccg cacctcatcc tgatgtttaa tttcaggaat gacgcttttt
    30241 gcaacgcgca cgataccatc agtatcattc actactgatt tcataaaaac tcctgattgt
//
LOCUS       DAZXUL010000044        29723 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZXUL010000044 DAZXUL010000000
VERSION     DAZXUL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29723
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.044"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1224
                     /locus_tag="ACH3L1_001947"
     CDS             <1..1224
                     /locus_tag="ACH3L1_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFT4076693.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="glpX"
                     /locus_tag="ACH3L1_001948"
     CDS             1221..2186
                     /gene="glpX"
                     /locus_tag="ACH3L1_001948"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFT4076694.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="ACH3L1_001949"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="ACH3L1_001949"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFT4076695.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="ACH3L1_001950"
     CDS             2714..3427
                     /locus_tag="ACH3L1_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFT4076696.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="ACH3L1_001951"
     CDS             complement(3399..4076)
                     /locus_tag="ACH3L1_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFT4076697.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="ACH3L1_001952"
     CDS             complement(4070..4747)
                     /locus_tag="ACH3L1_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFT4076698.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="ACH3L1_001953"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="ACH3L1_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5443..6021)
                     /locus_tag="ACH3L1_001954"
     CDS             complement(5443..6021)
                     /locus_tag="ACH3L1_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFT4076699.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="ACH3L1_001955"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="ACH3L1_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="ACH3L1_001956"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="ACH3L1_001956"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFT4076700.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="ACH3L1_001957"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="ACH3L1_001957"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFT4076701.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="ACH3L1_001958"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="ACH3L1_001958"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFT4076702.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="ACH3L1_001959"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="ACH3L1_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFT4076703.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="ACH3L1_001960"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="ACH3L1_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFT4076704.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="ACH3L1_001961"
     CDS             12291..13031
                     /locus_tag="ACH3L1_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFT4076705.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="ACH3L1_001962"
     CDS             13198..14148
                     /locus_tag="ACH3L1_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFT4076706.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACH3L1_001963"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACH3L1_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACH3L1_001964"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACH3L1_001964"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFT4076707.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACH3L1_001965"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACH3L1_001965"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFT4076708.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACH3L1_001966"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACH3L1_001966"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFT4076709.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACH3L1_001967"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACH3L1_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFT4076710.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="ACH3L1_001968"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="ACH3L1_001968"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFT4076711.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="ACH3L1_001969"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="ACH3L1_001969"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFT4076712.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="ACH3L1_001970"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="ACH3L1_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFT4076713.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="ACH3L1_001971"
     CDS             23768..23827
                     /locus_tag="ACH3L1_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFT4076714.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="ACH3L1_001972"
     CDS             complement(23928..24530)
                     /locus_tag="ACH3L1_001972"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFT4076715.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="ACH3L1_001973"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACH3L1_001973"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACH3L1_001974"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACH3L1_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFT4076716.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="ACH3L1_001975"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="ACH3L1_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFT4076717.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="ACH3L1_001976"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="ACH3L1_001976"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFT4076718.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="ACH3L1_001977"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="ACH3L1_001977"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFT4076719.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="ACH3L1_001978"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="ACH3L1_001978"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFT4076720.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
BASE COUNT         7095 a         7437 c         8024 g         7167 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tct
//
LOCUS       DAZXUL010000045        29466 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZXUL010000045 DAZXUL010000000
VERSION     DAZXUL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29466
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.045"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..213)
                     /locus_tag="ACH3L1_001979"
     CDS             complement(<1..213)
                     /locus_tag="ACH3L1_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFT4076721.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="ACH3L1_001980"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="ACH3L1_001980"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFT4076722.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="ACH3L1_001981"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="ACH3L1_001981"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFT4076723.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="ACH3L1_001982"
     CDS             2894..3634
                     /locus_tag="ACH3L1_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFT4076724.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="ACH3L1_001983"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="ACH3L1_001983"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFT4076725.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="ACH3L1_001984"
     CDS             complement(4192..4794)
                     /locus_tag="ACH3L1_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFT4076726.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4864..4929
                     /gene="yobI"
                     /locus_tag="ACH3L1_001985"
     CDS             4864..4929
                     /gene="yobI"
                     /locus_tag="ACH3L1_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFT4076727.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5068..5679
                     /gene="ruvA"
                     /locus_tag="ACH3L1_001986"
     CDS             5068..5679
                     /gene="ruvA"
                     /locus_tag="ACH3L1_001986"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFT4076728.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5688..6698
                     /gene="ruvB"
                     /locus_tag="ACH3L1_001987"
     CDS             5688..6698
                     /gene="ruvB"
                     /locus_tag="ACH3L1_001987"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFT4076729.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="ACH3L1_001988"
     CDS             complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="ACH3L1_001988"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFT4076730.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="ACH3L1_001989"
     CDS             complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="ACH3L1_001989"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFT4076731.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8461..9393
                     /gene="znuA"
                     /locus_tag="ACH3L1_001990"
     CDS             8461..9393
                     /gene="znuA"
                     /locus_tag="ACH3L1_001990"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFT4076732.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9409..10731
                     /gene="mepM"
                     /locus_tag="ACH3L1_001991"
     CDS             9409..10731
                     /gene="mepM"
                     /locus_tag="ACH3L1_001991"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFT4076733.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10851..11822
                     /gene="lpxM"
                     /locus_tag="ACH3L1_001992"
                     /gene_synonym="msbB"
     CDS             10851..11822
                     /gene="lpxM"
                     /locus_tag="ACH3L1_001992"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFT4076734.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="ACH3L1_001993"
     CDS             complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="ACH3L1_001993"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFT4076735.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="ACH3L1_001994"
                     /gene_synonym="yebK"
     CDS             complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="ACH3L1_001994"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFT4076736.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14729..16204
                     /gene="zwf"
                     /locus_tag="ACH3L1_001995"
     CDS             14729..16204
                     /gene="zwf"
                     /locus_tag="ACH3L1_001995"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFT4076737.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16439..18250
                     /gene="edd"
                     /locus_tag="ACH3L1_001996"
     CDS             16439..18250
                     /gene="edd"
                     /locus_tag="ACH3L1_001996"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFT4076738.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18287..18928
                     /gene="kdgA"
                     /locus_tag="ACH3L1_001997"
                     /gene_synonym="eda"
     CDS             18287..18928
                     /gene="kdgA"
                     /locus_tag="ACH3L1_001997"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFT4076739.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18984..20162)
                     /gene="purT"
                     /locus_tag="ACH3L1_001998"
     CDS             complement(18984..20162)
                     /gene="purT"
                     /locus_tag="ACH3L1_001998"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFT4076740.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20296..20586
                     /gene="yebG"
                     /locus_tag="ACH3L1_001999"
                     /pseudo
     CDS             20296..20586
                     /gene="yebG"
                     /locus_tag="ACH3L1_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20653..21009
                     /gene="yebF"
                     /locus_tag="ACH3L1_002000"
     CDS             20653..21009
                     /gene="yebF"
                     /locus_tag="ACH3L1_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFT4076741.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21336..21995
                     /locus_tag="ACH3L1_002001"
     CDS             21336..21995
                     /locus_tag="ACH3L1_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFT4076742.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22205..24265
                     /gene="ptrB"
                     /locus_tag="ACH3L1_002002"
     CDS             22205..24265
                     /gene="ptrB"
                     /locus_tag="ACH3L1_002002"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFT4076743.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="ACH3L1_002003"
     CDS             complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="ACH3L1_002003"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFT4076744.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24948..25604)
                     /gene="yobB"
                     /locus_tag="ACH3L1_002004"
                     /pseudo
     CDS             complement(24948..25604)
                     /gene="yobB"
                     /locus_tag="ACH3L1_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            complement(25706..25936)
                     /gene="holE"
                     /locus_tag="ACH3L1_002005"
     CDS             complement(25706..25936)
                     /gene="holE"
                     /locus_tag="ACH3L1_002005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFT4076745.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26075..26449
                     /gene="yobA"
                     /locus_tag="ACH3L1_002006"
     CDS             26075..26449
                     /gene="yobA"
                     /locus_tag="ACH3L1_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFT4076746.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26453..27325
                     /gene="copD"
                     /locus_tag="ACH3L1_002007"
                     /pseudo
     CDS             26453..27325
                     /gene="copD"
                     /locus_tag="ACH3L1_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27338..27679
                     /locus_tag="ACH3L1_002008"
     CDS             27338..27679
                     /locus_tag="ACH3L1_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFT4076747.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28072..29149)
                     /locus_tag="ACH3L1_002009"
                     /pseudo
     CDS             complement(28072..29149)
                     /locus_tag="ACH3L1_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(29115..29396)
                     /locus_tag="ACH3L1_002010"
     CDS             complement(29115..29396)
                     /locus_tag="ACH3L1_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFT4076748.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         7437 a         7235 c         7567 g         7227 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc
     4861 gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt
     4921 ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg
     4981 caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat
     5041 ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa
     5101 aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg
     5161 atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt
     5221 gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg
     5281 ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc
     5341 ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg
     5401 aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga
     5461 tttaaaggtt tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct
     5521 gctagcccgg cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc
     5581 tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt
     5641 gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag
     5701 accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc
     5761 ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca
     5821 tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg
     5881 ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca
     5941 cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg
     6001 aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag
     6061 tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg
     6121 cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag
     6181 gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc
     6241 aggtgccgga tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga
     6301 gtgatgacgg tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc
     6361 gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag
     6421 atatcgctgc tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg
     6481 accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata
     6541 acctggcggc agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt
     6601 tgattcagca aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt
     6661 ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg
     6721 catccgacac tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga
     6781 gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga
     6841 tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca
     6901 ccaccgacgg acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg
     6961 ccaccatccc caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac
     7021 gcgcagtagc tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca
     7081 tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt
     7141 tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt
     7201 gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg
     7261 gcgtcactgc cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca
     7321 tcagactaac gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg
     7381 tgtcgatcgc cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg
     7441 taacggcaat caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa
     7501 gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg
     7561 aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata
     7621 attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg
     7681 atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga
     7741 aataaactcc ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt
     7801 caggcacagc acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa
     7861 aacgccacaa tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc
     7921 atttacatcc acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa
     7981 tgctcgcgct aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt
     8041 aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc
     8101 agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt
     8161 ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt
     8221 taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa
     8281 agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc
     8341 aaaagaaacc gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca
     8401 gtagtcatga aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt
     8461 atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca
     8521 acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct
     8581 gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat
     8641 gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg
     8701 gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag
     8761 caggtaacga ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc
     8821 gatgatgatg atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc
     8881 aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga
     8941 aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt
     9001 gaggcacaat tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg
     9061 aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca
     9121 ccgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa
     9181 ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg
     9241 ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc
     9301 ctggggacga atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc
     9361 aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata
     9421 gcccgctctg tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg
     9481 tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat
     9541 gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct
     9601 gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa
     9661 ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat
     9721 acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct
     9781 gctgccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc
     9841 gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat
     9901 gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt
     9961 aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt
    10021 ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc
    10081 aaactgaaaa aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa
    10141 cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca
    10201 atccgcgctg aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc
    10261 ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact
    10321 aatccggtga caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt
    10381 acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca
    10441 ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc
    10501 aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt
    10561 aataccggac gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc
    10621 gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc
    10681 gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc
    10741 attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc
    10801 cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga
    10861 aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact
    10921 ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc
    10981 gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc
    11041 gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag
    11101 cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg
    11161 caatacgcgg gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg
    11221 aagagatgcg gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg
    11281 atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc
    11341 agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc
    11401 tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg
    11461 gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg
    11521 ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg
    11581 ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc
    11641 caccgatgga tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag
    11701 aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga
    11761 aaactcgcaa accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat
    11821 aaaaaagcct ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa
    11881 tattcgaaat ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat
    11941 ccggcaacgt acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact
    12001 catcacgtcg ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg
    12061 cagcagatta accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac
    12121 cggcgtaacg ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc
    12181 gaaaattggt agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt
    12241 catggtaatg atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc
    12301 aatagcttct tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct
    12361 cgggattttt tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata
    12421 ctgcccagcg gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt
    12481 tgctacgtcc atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat
    12541 ctgggtcgcc gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg
    12601 aatgccgacc agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac
    12661 cacgtcagag gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg
    12721 ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata
    12781 gttcagatct tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc
    12841 cgcagtctta atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc
    12901 aagtttgttg atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac
    12961 tttcatgccc tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat
    13021 gtcaccaggc acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc
    13081 gccttcacct ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac
    13141 tttgccttct ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc
    13201 cacatgacgc cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc
    13261 ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt
    13321 ttcaagatta ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg
    13381 aagccttttg gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga
    13441 aatccgaatg aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag
    13501 attaggacag tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa
    13561 aacgcgattc cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc
    13621 gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa
    13681 ccatgggcat ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt
    13741 cccgggcgag cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg
    13801 ccagctgcgc cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca
    13861 cgtctccgtc gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca
    13921 ccaccggaac attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag
    13981 agcctaatcc gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga
    14041 tggcagattt atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata
    14101 ttttccccgt gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag
    14161 tgccattcgc cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca
    14221 tgctgcgaca gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag
    14281 caatactcga atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct
    14341 ctgatttgct caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa
    14401 gagactcacg ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga
    14461 tattacgcct gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca
    14521 taacatgatc agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg
    14581 ccgcgctttg cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca
    14641 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
    14701 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga
    14761 cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
    14821 tcaactggaa aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    14881 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    14941 agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct
    15001 cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg
    15061 tatcactatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    15121 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    15181 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    15241 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    15301 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    15361 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    15421 gatgcgtgac atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc
    15481 gccatctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct
    15541 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    15601 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag
    15661 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    15721 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    15781 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    15841 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    15901 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    15961 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    16021 tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc
    16081 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    16141 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    16201 gtaatatcag cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg
    16261 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    16321 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    16381 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    16441 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    16501 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    16561 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa
    16621 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca
    16681 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    16741 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    16801 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc
    16861 ccataacatg tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct
    16921 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    16981 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    17041 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    17101 tactttctac ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt
    17161 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    17221 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    17281 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    17341 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    17401 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc
    17461 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    17521 caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    17581 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    17641 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    17701 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    17761 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    17821 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    17881 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    17941 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    18001 agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg
    18061 ggacatcatt cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga
    18121 actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    18181 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    18241 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    18301 aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    18361 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    18421 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    18481 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    18541 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    18601 cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    18661 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    18721 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    18781 tccggctaac taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg
    18841 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    18901 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt
    18961 tttgtagcgt ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    19021 gcgcgttcaa tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg
    19081 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    19141 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    19201 gcaggaccat actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg
    19261 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    19321 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    19381 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    19441 gggctcattt gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct
    19501 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    19561 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    19621 gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg
    19681 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    19741 aaaaggcttt tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    19801 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    19861 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    19921 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    19981 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg
    20041 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    20101 ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc
    20161 ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc
    20221 tttattgctg atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat
    20281 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    20341 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    20401 gatctgcttg acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    20461 gccctttcgc tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc
    20521 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    20581 gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    20641 atggagaaaa acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc
    20701 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    20761 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    20821 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt
    20881 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa
    20941 agtgccgata ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag
    21001 cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    21061 tccgtcccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    21121 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    21181 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    21241 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg
    21301 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    21361 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    21421 aactgttagt gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag
    21481 cacgtaaact tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag
    21541 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    21601 cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    21661 tggtctttgc cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc
    21721 agcaattgcg tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg
    21781 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    21841 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    21901 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    21961 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    22021 tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct
    22081 gcataacatt cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt
    22141 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa
    22201 taacatgcta ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg
    22261 catcgataat tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta
    22321 cctgcaacaa gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga
    22381 tcgcatctta aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat
    22441 aaaaaatggc taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca
    22501 gcgtcaatcg gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa
    22561 gcgcgcagct catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac
    22621 cattatggcg ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa
    22681 tctggaaaca ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg
    22741 ggcaaatgac tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta
    22801 tcaggtctgg cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga
    22861 aaaagacgat acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat
    22921 tcatctggcc agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc
    22981 cgagccgttt gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca
    23041 gcatcgtttt tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg
    23101 tatgcgtgat gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga
    23161 agggtttacg ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag
    23221 tttgcgccaa attaaccgca agacccggga agtcattggc attgcctttg atgatccggc
    23281 ctatgtgacc tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta
    23341 ttcttccatg accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg
    23401 tgtattaaaa caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct
    23461 gtggatagtc gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca
    23521 ttttcgcaaa ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat
    23581 tgatgccgat ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat
    23641 tgtccatgtt cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct
    23701 gaagaagaaa aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg
    23761 ctatggctct ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg
    23821 cgttgcaatt aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt
    23881 tgatgttgta acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga
    23941 gtggggaaat ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga
    24001 caacgtcacc gcacaggctt atccgcattt actggtaacg accggtttac acgattctca
    24061 ggtgcaatat tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga
    24121 ccatctttta ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt
    24181 taaatcgtac gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac
    24241 attacccgct acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag
    24301 ctccgggctc atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc
    24361 ggcaacgtcg gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga
    24421 cggacgtccg gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc
    24481 gataagcaac gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag
    24541 cggtgtctgt acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc
    24601 tggccacaaa cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag
    24661 tacgcggcgg tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg
    24721 gatcacatct tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc
    24781 catcgcttgt ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat
    24841 ttttccgtca atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt
    24901 ttctgtatcg ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga
    24961 atgatatcgc cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga
    25021 tcggcgcgaa cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt
    25081 gccatcagca cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc
    25141 agcaggtcgg gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata
    25201 tccatacctt gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat
    25261 gcaccatgac tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt
    25321 ccacgaataa agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag
    25381 gtcgttgcgg cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca
    25441 cgtcgcgaat aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt
    25501 tgcctggcgg cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt
    25561 ttacgaggct catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca
    25621 aaatgactgc aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt
    25681 aagaaagtgt aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca
    25741 gattgaccga agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt
    25801 ctggctgttc acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag
    25861 ccaccccggc cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag
    25921 ccagattctt cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga
    25981 gcctggatgg caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc
    26041 tgtaaaaaag ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg
    26101 ctacgcgctg gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca
    26161 tttaacgcat cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac
    26221 tttaaacttc tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa
    26281 aaacgaaaat attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat
    26341 tgttccactg gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc
    26401 ggtggacggg cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc
    26461 gtttacctgg atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt
    26521 tgcaatgtat ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc
    26581 tctgcgttta caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc
    26641 cgttcagggc gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca
    26701 ggctgttttg caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt
    26761 aacgttgatc gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac
    26821 taccgcgcaa tttattctgc tggcgggagt ggggtatgcg acgctgaatg aaggggtaac
    26881 agcgaaaatc catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg
    26941 gggattactg ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc
    27001 tattcaggcg ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc
    27061 aagcggcgtg cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg
    27121 gggccaactc ttattgctca aagccatcct gttgatgatt atggtggtca ttgcgctggc
    27181 taatcgttat gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt
    27241 tgtgtggatg actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct
    27301 gcttgcgact ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc
    27361 gtttctgtta ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag
    27421 tcgttttgaa gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg
    27481 ccgaccgggt aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa
    27541 cgatatcgca caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat
    27601 tcagattgat gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg
    27661 agctgaaaaa ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc
    27721 tcagttcttt caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt
    27781 tattgaccat tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca
    27841 aggcaactaa gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt
    27901 tccctggacc gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt
    27961 agttgcaaac gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt
    28021 ttttggttat atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag
    28081 taagtacgtt ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc
    28141 aagtagacgc tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct
    28201 tctgatttcg tgaaaagttg gtgggttttc actgaacagt gatacctgtt aattttctgg
    28261 ctgcgacaaa tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg ctgttttttc
    28321 taatcccggc actgattaga taatcccccc tacttaccat gcggcactgt tcaactactg
    28381 caccaagtcg taaaccagcg acaggaaggc tgagtgacag ggggatagca atcatcattc
    28441 ctgtcttaat ttgcctgatg tggagacgat catcataaat atcactgaac cgcatattcg
    28501 ttatgtcttc gcgacgttgt cctgttacaa gggctaagtc catagctaat gggaaccatg
    28561 ccggaagttg atctgctgcc tccctgatgc agttgtatgt ctttagtttc agccgttctc
    28621 ttgtaactac tattttcggt gctcttgttg gcgttactgg attttgagat atacgtcctt
    28681 caacaatggc ttcgcggaac atatcagata acacagaacg cattgatcct gccatcgtgt
    28741 ttttccctcc ttcaatctac aaatcaagaa actcggcgat atggcgagtg gttatttctg
    28801 tcagtaaaac ccctcctaat ttttctttta ttgtctccag ttgatttacc cgagttttat
    28861 aagtatttct ggacgctttt ctccttataa gaatcgtttt gtaacgttca atccagcctg
    28921 ccatagtaaa tgagtcgacc cctttaagct tttcaattag ggcagcagga gagtagtttt
    28981 tgtatatata atgatttgct tcaattgcct gcgctactgc atctcttctt gaaattttac
    29041 ctagtgtaaa ttcttctttc gtcagagggt tgcgccagta atatgctttg tctctccttc
    29101 gatatgttaa gtttctaggc aaattgggat cgtatttttt ccgctgcatg ttttaacttc
    29161 tccattaacg gactgtctct cccttgtcgc ccattaggct gatggtgtgt tatatcggta
    29221 tcaaccttat ttgggttgat atagaaagcc tccggaacca ccctgtaact cctcccgtgt
    29281 agttcaggtg caggataaat gtttccattc cttgcccatc gtctcagcgt tgatattgat
    29341 ggtgggttat ccggatatct gagttttccc cacgttttga gtgtcacaag attcattgcc
    29401 atacctctca cgatatgacc gccagtaaat atacagaata ctggcgggtg tggttgattt
    29461 ttaata
//
LOCUS       DAZXUL010000046        28908 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZXUL010000046 DAZXUL010000000
VERSION     DAZXUL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28908
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.046"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACH3L1_002011"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACH3L1_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFT4076749.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="ACH3L1_002012"
     CDS             complement(2138..2584)
                     /locus_tag="ACH3L1_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFT4076750.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="ACH3L1_002013"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="ACH3L1_002013"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFT4076751.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACH3L1_002014"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACH3L1_002014"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFT4076752.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="ACH3L1_002015"
     CDS             complement(5347..6009)
                     /locus_tag="ACH3L1_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFT4076753.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="ACH3L1_002016"
     CDS             6182..6595
                     /locus_tag="ACH3L1_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFT4076754.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACH3L1_002017"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACH3L1_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFT4076755.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACH3L1_002018"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACH3L1_002018"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFT4076756.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="ACH3L1_002019"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="ACH3L1_002019"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFT4076757.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACH3L1_002020"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACH3L1_002020"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFT4076758.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACH3L1_002021"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACH3L1_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFT4076759.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="ACH3L1_002022"
     tRNA            complement(9861..9948)
                     /locus_tag="ACH3L1_002022"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="ACH3L1_002023"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="ACH3L1_002023"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFT4076760.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="ACH3L1_002024"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="ACH3L1_002024"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFT4076761.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="ACH3L1_002025"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="ACH3L1_002025"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFT4076762.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /gene="hyaD"
                     /locus_tag="ACH3L1_002026"
     CDS             14005..14592
                     /gene="hyaD"
                     /locus_tag="ACH3L1_002026"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFT4076763.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="ACH3L1_002027"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="ACH3L1_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFT4076764.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="ACH3L1_002028"
     CDS             14984..15841
                     /locus_tag="ACH3L1_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFT4076765.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="ACH3L1_002029"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="ACH3L1_002029"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFT4076766.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="ACH3L1_002030"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="ACH3L1_002030"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFT4076767.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="ACH3L1_002031"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="ACH3L1_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFT4076768.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="ACH3L1_002032"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="ACH3L1_002032"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFT4076769.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACH3L1_002033"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACH3L1_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACH3L1_002034"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACH3L1_002034"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFT4076770.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="ACH3L1_002035"
     CDS             complement(22884..24023)
                     /locus_tag="ACH3L1_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFT4076771.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="ACH3L1_002036"
     CDS             complement(24069..26165)
                     /locus_tag="ACH3L1_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFT4076772.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26905)
                     /locus_tag="ACH3L1_002037"
     CDS             complement(26165..26905)
                     /locus_tag="ACH3L1_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFT4076773.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAGQL
                     PWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQLWL
                     GFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26902..27546)
                     /gene="gfcB"
                     /locus_tag="ACH3L1_002038"
     CDS             complement(26902..27546)
                     /gene="gfcB"
                     /locus_tag="ACH3L1_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFT4076774.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27653..27958)
                     /locus_tag="ACH3L1_002039"
     CDS             complement(27653..27958)
                     /locus_tag="ACH3L1_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076775.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28400..28612)
                     /gene="cspH"
                     /locus_tag="ACH3L1_002040"
     CDS             complement(28400..28612)
                     /gene="cspH"
                     /locus_tag="ACH3L1_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFT4076776.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7001 a         7320 c         7598 g         6989 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagtaactg ctggcgcacg gatttaatcg ttgccgccac atcgtcgtca gcttctgctt
    26641 cccaggaagc aagctgtgcc atgacatgtt ggtagtcttt aagcgcttta gctttcgccg
    26701 cagagtcggt cagcaaggca ccaggccacc agaggcgatc gcgcagttgc ggttgtgtca
    26761 ccaactggac aacattttcc acggggccaa cggaaagtgt ctgttgttcg ctaggcagat
    26821 aaatagtcac cgttccttgc gcaaaggcat ggggtgtcat tacgtaaagt acgctggcaa
    26881 tgaaatacga ctgtaattta ttcatggtgc gggtttaaga aacgtcattt ctacgggaat
    26941 aacccctgcg cctaacattt gccgactttg acgaacctgg ccagtagccg aatcgaccca
    27001 gaatgtgttt tgccactgtt tttctggcag cgtggaagtc acctcttcct gccagacatt
    27061 acacagcaca ttgtgacccg caatattcag cgtctctaat ccagcaaagg aaaaagtaga
    27121 acttagggtc gcagagcgaa aatggttgtc ttcactccag agaatatcgc gcgtccagcg
    27181 gctgccatct tttattgcca gcgcgttacg cagaggggcc cgcccgggat ttgtcacttc
    27241 cagcagatta ttattaagtt tgacggtctt taaaagacgt ccattgtggg taaccagcat
    27301 ggcgttatcc tgggataacc atttgctttg ttcttgttct atatatccca gtaccacaaa
    27361 gattcgttgc ccttcattaa ggcgtaaata catcgtggaa taaggcaacg cctggatctg
    27421 ctgagccgct acagtgatat cctgattatc gaaaaggctg gcgcgaaatg tatcgaccat
    27481 actttgctgg ctgtgcgtac atcccgcaag aaatagtgcg aaaatcgata aaataagagg
    27541 gcgcaccttt aatccttgaa aggttaatta aaaaaatgca aatctctaaa gcccggtacg
    27601 aatatcgcaa ccgggtatta tttaacttga atttcgacgc gtttaagcag aattagcgcg
    27661 tagtggacgt ggtggttgag gtgttatgag aaccgccatc gccactgtca tttgctgcaa
    27721 ctactgccaa cgtcgccaca ccaacagccg ttgccacacc gacaactgtc gccgtgccga
    27781 cagcgccagc agccacgcca ctagtggtag ccgcagtcgt ggttgcacct gtagtggtcg
    27841 ttgtggtgcc agtagtaccc gtcgttgcct cggtcccgtt tgctgcttca ggggcagcaa
    27901 acgcagctgg cgtcgtcaac atgaaggcca taaggattgc agaaagcttg tgtttcataa
    27961 cttttccttt attcatcgca tggacaatac gtacgcttga gatcaggatt gtctactttg
    28021 agtataacat ccttgtgaaa tctccctttc ctcataccgc tagcactata tactgcgggt
    28081 gggaaaggcg cgcagaggga aatataagat tgttaacgat ttccctctgg aaatacgact
    28141 tgtgagcata agataaatct tagaaaaata cgaattaaag ataagaaaat tgttacgaaa
    28201 gctattaatt attgttggta atatagtttc aagtggtact ataactggct gttaaatggc
    28261 atttataact attaggtgca gagatattcg cttaaatgga ttagtttctt agtggaaaag
    28321 taactgatgt tataatgtat ggtgttgact attgaactat tccaataaaa ccagtcagct
    28381 ttaaacaagc agcgtcatat taagagagat aaacatttgc cgctgttggt cctcgcaggc
    28441 catttacgcg gcaaaattcc acgcgtaatc ctggtataag cacttctgcg tcgcggggag
    28501 tgaatgcgga aatatggacc tggacttctt tacgaccgtc ggaggggata atgaatcctt
    28561 tgccgctttt gcgatcaaag gttttgacaa ttcctgtcat tttacgggac aaacaaattc
    28621 cttactgaaa atactgcgct gcactatacg gggttaataa aataaagcca gcgatattta
    28681 agaccgccgg acggctaaaa taaaatttgc ttaatctcaa ttatcatgcc ttaatagctg
    28741 cgtcggtttg aaagacagac agcatacaaa gtagtttact aaagcagttc tcattatcag
    28801 gcattatccc cttcttttga gtctctctcc tgaacactaa gtggtttctg tattaaagcc
    28861 ctgtttgccg aaaggcccaa aatgaaggaa gtaaaatatg tctaataa
//
LOCUS       DAZXUL010000047        28670 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZXUL010000047 DAZXUL010000000
VERSION     DAZXUL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28670
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.047"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            164..649
                     /locus_tag="ACH3L1_002041"
     CDS             164..649
                     /locus_tag="ACH3L1_002041"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076777.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLLNVT
                     K"
     gene            646..1032
                     /locus_tag="ACH3L1_002042"
     CDS             646..1032
                     /locus_tag="ACH3L1_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076778.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1057..3270
                     /locus_tag="ACH3L1_002043"
     CDS             1057..3270
                     /locus_tag="ACH3L1_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFT4076779.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3293..3738
                     /locus_tag="ACH3L1_002044"
                     /pseudo
     CDS             3293..3738
                     /locus_tag="ACH3L1_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3760..7318
                     /locus_tag="ACH3L1_002045"
                     /pseudo
     CDS             3760..7318
                     /locus_tag="ACH3L1_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            7538..7741
                     /locus_tag="ACH3L1_002046"
     CDS             7538..7741
                     /locus_tag="ACH3L1_002046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076780.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7759..8079
                     /locus_tag="ACH3L1_002047"
     CDS             7759..8079
                     /locus_tag="ACH3L1_002047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076781.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8145..8377)
                     /locus_tag="ACH3L1_002048"
                     /pseudo
     CDS             complement(8145..8377)
                     /locus_tag="ACH3L1_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8487..9257)
                     /gene="yafV"
                     /locus_tag="ACH3L1_002049"
     CDS             complement(8487..9257)
                     /gene="yafV"
                     /locus_tag="ACH3L1_002049"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFT4076782.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9438..9884
                     /gene="ivy"
                     /locus_tag="ACH3L1_002050"
     CDS             9438..9884
                     /gene="ivy"
                     /locus_tag="ACH3L1_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFT4076783.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9927..12371)
                     /gene="fadE"
                     /locus_tag="ACH3L1_002051"
     CDS             complement(9927..12371)
                     /gene="fadE"
                     /locus_tag="ACH3L1_002051"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFT4076784.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12611..13189
                     /gene="lpcA"
                     /locus_tag="ACH3L1_002052"
     CDS             12611..13189
                     /gene="lpcA"
                     /locus_tag="ACH3L1_002052"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFT4076785.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13395..14162
                     /locus_tag="ACH3L1_002053"
     CDS             13395..14162
                     /locus_tag="ACH3L1_002053"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFT4076786.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14133..14873)
                     /gene="dpaA"
                     /locus_tag="ACH3L1_002054"
     CDS             complement(14133..14873)
                     /gene="dpaA"
                     /locus_tag="ACH3L1_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFT4076787.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15257..15778
                     /locus_tag="ACH3L1_002055"
                     /pseudo
     CDS             15257..15778
                     /locus_tag="ACH3L1_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15954..16451
                     /gene="rayT"
                     /locus_tag="ACH3L1_002056"
     CDS             15954..16451
                     /gene="rayT"
                     /locus_tag="ACH3L1_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFT4076788.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16668..18380)
                     /locus_tag="ACH3L1_002057"
                     /pseudo
     CDS             complement(16668..18380)
                     /locus_tag="ACH3L1_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18352..19137
                     /gene="lafU"
                     /locus_tag="ACH3L1_002058"
     CDS             18352..19137
                     /gene="lafU"
                     /locus_tag="ACH3L1_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFT4076789.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19208..20263
                     /gene="dinB"
                     /locus_tag="ACH3L1_002059"
     CDS             19208..20263
                     /gene="dinB"
                     /locus_tag="ACH3L1_002059"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFT4076790.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20315..20608
                     /gene="yafN"
                     /locus_tag="ACH3L1_002060"
     CDS             20315..20608
                     /gene="yafN"
                     /locus_tag="ACH3L1_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFT4076791.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20611..21009
                     /gene="yafO"
                     /locus_tag="ACH3L1_002061"
     CDS             20611..21009
                     /gene="yafO"
                     /locus_tag="ACH3L1_002061"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFT4076792.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21019..21471
                     /locus_tag="ACH3L1_002062"
     CDS             21019..21471
                     /locus_tag="ACH3L1_002062"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFT4076793.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21717..22760
                     /locus_tag="ACH3L1_002063"
     CDS             21717..22760
                     /locus_tag="ACH3L1_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFT4076794.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22822..23436
                     /gene="prfH"
                     /locus_tag="ACH3L1_002064"
                     /pseudo
     CDS             22822..23436
                     /gene="prfH"
                     /locus_tag="ACH3L1_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23493..24950)
                     /gene="pepD"
                     /locus_tag="ACH3L1_002065"
     CDS             complement(23493..24950)
                     /gene="pepD"
                     /locus_tag="ACH3L1_002065"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFT4076795.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25212..25670
                     /gene="gpt"
                     /locus_tag="ACH3L1_002066"
     CDS             25212..25670
                     /gene="gpt"
                     /locus_tag="ACH3L1_002066"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFT4076796.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25762..27006
                     /gene="frsA"
                     /locus_tag="ACH3L1_002067"
     CDS             25762..27006
                     /gene="frsA"
                     /locus_tag="ACH3L1_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFT4076797.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27064..27465
                     /gene="crl"
                     /locus_tag="ACH3L1_002068"
     CDS             27064..27465
                     /gene="crl"
                     /locus_tag="ACH3L1_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFT4076798.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27504..28559)
                     /gene="phoE"
                     /locus_tag="ACH3L1_002069"
     CDS             complement(27504..28559)
                     /gene="phoE"
                     /locus_tag="ACH3L1_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFT4076799.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7083 a         7123 c         7606 g         6858 t
ORIGIN      
        1 aacaaatatt gatggcaata tcattataca taaggatggt aggaggacgt atttattaaa
       61 tcaaaatact ggttcgatta cggctgtgat aagatggaag tatgagtagt ataaggataa
      121 taccgttaca gatgactgga ctatatggga aaaagctatt tttatgcgag cgatatattt
      181 atctgttcag caggcatgga atgggaaaat aacttattct gtttccggtg aatctgagtt
      241 cgctaaaaaa tttcagggta aagctttgcc ttttgatgta agaataattt ctgcatctca
      301 gaatgaagac tggttggtta ttgcaacaaa ggttttgcct ggtgctgatt taagaacata
      361 tgttgatttt aaaaacagta cggttcatgt tgattcagcg ggcctggaaa aagtagcgaa
      421 atgtattaac tgtaataata cgttgcaagt aaatatacct catgaagccg gacatgtact
      481 tggttactta gatgatgatt atgattcttc gtctccgtat gttggagata ttagtgggtt
      541 gatgaatgtg ggaatggagc tttgggaacg ataccttaaa aacgcaacta taacgttgaa
      601 tatcataatg cctgaaacta aatttacttt attgaatgta accaaatgaa atttttctgt
      661 attatagtga ttttcctttc aataaattac atagctatag tgaatgtttt caagtcttct
      721 gcagttgctc aatccaccaa atattatgaa gaaaatgata tacatgatgg aagtaatgac
      781 atctttttta tgaagaaata tgggccgcct gctcatgagt tcgtgaggcc acgatataaa
      841 tttgttggtg agtattatat cggcttgaat caaacttatc gcataacaga tcctgaattt
      901 caagatgttc ctattaagga aatgttctgg aatttgcagg gggatttgaa tctgacatgt
      961 tggtttcatt ataaagacgg gcaatggaga gtgattactt atattttttg gccaccaggg
     1021 gctagatttt aatccggtat taaaggagca aatatcatga gttttgtatc tacgggtaat
     1081 aaaccagttg gaaacggtgg acccgtcatc accacaccac caatagctgg cgaaagtggc
     1141 ggtatgagca caggttcagc tgtaaccgat gtatctggtg ccgccgagga gatggcagaa
     1201 caggctgcgg cggacctgtt tggtgcgctg ccggaaccgt caggtctggt taaagccgca
     1261 gtagctgctg cgcaggctgc cgctgcagca gcagggatct cggatatggc tggtgcagtt
     1321 caggatgcag cggcgtcgct tgcagccggt gctccggggg cgcataacgt cacagtaagt
     1381 ggtagtgcgg tgccgccgca gatgcttttg tttgcaggca tgaatggttc ggaagggctt
     1441 gggaatctgt tttcttacac agtacagctt aaaacgctgg acgccctgaa cctggggtac
     1501 gtttcccccg ctgctaacct gccgcttcag ctcatggtgg gcaaagacct gtgcgtcagt
     1561 atcgaactgg acggcggcgg aaaacggtat atcagcgggc tggtgacggc ggcgagggtg
     1621 gtcggccatg agagccgttc ggtaacgtat gaattacgca ttgagccatg gctgaagctg
     1681 ctgacccata ccagcgacta caaagcgttc cagaacaaaa ccgtggtgga tattctcgat
     1741 gaagtactgg atgaatattc ctttccggtg gaaaagcggc tggtggaaaa ttatccaccc
     1801 cgcgcctggc aggtgcagta tggtgaaacc gacttcgatt tcatccagcg cctgatgcag
     1861 gaatggggga tttactggtg gtttgagcac agcgagaaca gccacacgct ggtgctggtg
     1921 gatgccatca atggtcataa agcgtgccct gactcgccgc tggtggagtg gcaccaggaa
     1981 gggctgaaac tggataagga atttatccac accattacgg ccagtgagag gctgcgtact
     2041 ggaaagtggg tgatggacga ttttgatttt atgaagcctc gttcgctgct taaaagtacc
     2101 gtggccagtc cgcgtgatac cggtcatgct gaatacgagc attacgaatg gcctggtgat
     2161 tacttcacta cgggcgaagg cgagatgctg acgcgcattc gtatggaagc gcagcgcagc
     2221 ccaggcagcc gggcacatgg ggccggtcat atccgtaccc tcatgaccgg gtacaccttt
     2281 acgttgatga atcacccgac ggcagaaatt aatcaggaat atctgctggt acagaccact
     2341 ctgtttttac gggataatgc gcagcacagc ggtcaggatc agcattttac ctatgtcacc
     2401 acgtttgaac tgcaccccac cagcgaagtt taccgcccac aacgaaccct gagcaaaccc
     2461 cacaccaaag gcccgcagag cgccatcgtc accggcccgg cggggcagga aatctggacg
     2521 gataaatatg gccgggtgaa ggtgcagttt ggctgggacc gttacggaaa gaatgacgaa
     2581 aacagttcct gctggatccg cgtcagctat ccgtgggcgg gtaaaggatt cggcatgatc
     2641 cagatcccgc gtattggcca ggaagtactg gtggatttca aaaacggcga tccggatttg
     2701 cccatcatcg tggggcgcac ctataaccag gacaccatgc cgccgtgggg gctgccgggc
     2761 atggcgtcgc agagtgggat attcagccac tcattgcagg gagggccgac taacggaaat
     2821 atgctgcgct tcgatgataa aacgggtgcg gaagaggtga agttccatgc ggaaaaagac
     2881 ctcaatacca cggtaaaaaa caatgaaacc cacacggtca tggtcgatcg cataaaaacc
     2941 attgtcaaaa atgaaaccat cagcgtggga gaagaccgta atgccaccat cacaaaaaat
     3001 gatggtcttt ccgtcaaact ggctcaaacc atcaatgtcg gaacgacata tcgtctggat
     3061 gttggcgatc aatttaccct tcgttgcggc aatgcagcgc ttgtattaca caaggatggc
     3121 tcgattgagt tttgcggaaa acaactgtta ctgcacacca gcgatgttat gcaactcatt
     3181 ggcagtggca ttgatatgaa tccggatggc ggcacagcca tgactgcgga tgatattgcc
     3241 ccgttaccta cccccgacgt ttctgaataa aaaatataaa aggaattttt ctatgaccag
     3301 cctttaccgt attcaggagg gctgctttgc cctgcctgaa acttttcttg atcgcaccgt
     3361 caatatcttc gtcccatcgg gcaatgaaag ggcaactccc agtctgaata ttttccgcga
     3421 tacgctcagg ccagatgaaa atctgaccac ctatattgac cgccagatag ccttgatgaa
     3481 aaaaaaaacc taggccagca ccgggtactt tcgcggggaa cggcactggc gggaactggc
     3541 gaagctgcca tgacaggtga acagattctc gcaactcatc gttctggaaa aacagaagtg
     3601 taccagcgtc aggccgggtt tatcaccggg ccagcaaagg tactaatgtt gacgctgacc
     3661 actcagcgtc cttttgatga tcacaccgac cagctatgga ctgcatggtt gactagtttt
     3721 cagcctgcta aaagctaaaa aaaacaggga ggttttgtca tgtatgaagc cgcacgggtg
     3781 gatgacccca tctaccacac cagcgccctt gccgggtttt tgataggggc catcataggt
     3841 atcgccatta ttgccgtcgc cagctttgct ttttttacct gcggcttttt ggccgggctg
     3901 attttgagtt ttctggctga tcagatagct tccggagtcc tgcaactggg tgaggctatt
     3961 gggcgctcga tacattccac agtgggaaaa attctgaccg gttctgataa tgtcagcacc
     4021 aacagtcgcc cggccgcccg ggccgtactg agtacggtga tatgcgaaga ccattccccg
     4081 gaaatccgca ttgcccaagg ttccggcaac atctacatca acagccagct gcccgtaagg
     4141 atgaccacac tgagtgtgat gcagtgattg aggacggctc ccccaatgtc tttctcggcg
     4201 gcggtacgca gacggtactg gaaatcagcc cggaaatccc tgactggctg cgtcaggtgg
     4261 tggatgtgct gtatgtggtc gccggaatgc ttggcgggct ggctggcgcc tggcgtcagg
     4321 cggcaaagat ggggtcgaaa tttggtacaa aatgtgcggc aaaatttatt ggtggcgaaa
     4381 tggtcgggat ggccatctct gacacagtca tgggattgtt cagcaatccg gtagatgtca
     4441 ccactgggca aaaaattttg ctgccggaga cggattttac cctgccagga cgcctgcctg
     4501 tcacctgttc gcgcttttac gccagccacc tggaaactga aggattgctg ggcagaggat
     4561 ggcggctgaa ctgggagata aacctgcgtg aggatgaaac atatatcacc ttcatcggtg
     4621 tacagggtcg ggaactgagt tatccgaaag agatgctgat accgggccac cagatattcg
     4681 acccggaaga gcagttttac ctgagccgtc tgcatgacgg atgctatgtg ctgcattaca
     4741 ccgactgcag ctattacgtg tttgatgaat ttgatgacca cggcgtcgcg ccgttacttt
     4801 ttatggagac gccgtaccgg cagcgtattg ctttcgggcg tgagaacggc aggctggtgc
     4861 gggtggcctc cagcagcgga caccatctgt tattgcaccg cacgatgacg caagcagggg
     4921 aacgcctgtc gcatattgaa cttttgaagg gcgggcgtcc cggtaacctg gtggaatatc
     4981 gttacgacga taacgggcaa ctgactggcg tggtgaaccg ggctggtgtg acggtgcgcc
     5041 agtttgccta tgaaaatggc ctgatgacag agcatcgcaa cgcgacgggc tttacctgta
     5101 cctaccactg ggaagagatt gaaggtttcc cgcgcgtggt ggaacacacg accagcgacg
     5161 gcgaggatta ccggttccac tatgattttg ctggcgggca gacggtggta accggcaggc
     5221 cggagcagaa gtggcagtgg tggtttgatg aagagacgta tgtgactgcg caccggacgc
     5281 cgggcggcgg aatgtaccgc ttcacgtaca acgaaaacca ttttccggta gcggtggaac
     5341 tccccggcga acgcagggta acgctggaat acgacaccct gagccgggtg gtgaaagaga
     5401 cggacccggc aggccgggtg acacagacgc agtggaacgg cagttttgct gaaatcacgc
     5461 gccgggcgct ggatgacgac cacgtatgga aggcggatta caacgaacac ggccaggtga
     5521 tacgggagac ggacccggaa gggcgcgtca cgcggtacgg gtatgatgat caggggctgc
     5581 cggagacggt ctgccacccc ggtaaacagc aggaccgtta cacctggaac gcgctggggt
     5641 tactgagcag ccaccgtcgc atcacgggca gtgtgcagtc ctggcagtat acgcagcgcg
     5701 ggatgctggc gcgccatacg gatgaagaga aacgggagac gcgctggcag tacaccccgg
     5761 aagggctggt tgccagtctg agcaacggca acggggctca gtaccggttc agctacgacg
     5821 gcgacggacg gctgacggga gaacagcgcc cggacggcct gataaggatg tttgcgctga
     5881 atgcggacgg gtttccggtc atcatcccga cgcagggcac agagggcggg gtgcgcaatg
     5941 aacagcagga gcgtgatgcg ctgggccgcc tgctgcgcag tgacacgcag cacagtacac
     6001 ggaccttcag ctacaaccgt cttgaccaga taacggaagt gacgctcacc cccacggaag
     6061 aaggggaacg gctgcaccac atgcaggcgg acacggtgcg ttttgcgtat gaccgcagcg
     6121 ggtggctgac ggcagagcac agcgtgcacg gcagtatcaa ataccggcgc gatgcgctgg
     6181 gcaatcccac cgatatcacg ttgccggacg ggcagcatct cagtcatctt tactacggca
     6241 gcgggcatct gctccagacg gcactggacg gtatcacggt cagtgaatat gagcgtgaca
     6301 gcctgcaccg tcaggtgata cgcacgcagg ggaaactggc gacgttcagc ggctacaacg
     6361 ccgacaaccg gttaagctgg cagcgcagcc tgccaggtgg cagccagaat ccacagcagg
     6421 cggtactccc ccggcgcaga acgacagcgt gacggcgcgg gactatatct ggaacgcaga
     6481 cggtgaagtc ggcggcatca atgacaaact gcgcggttgc ctggtgttca gctatgaccg
     6541 cagcggctgg ctgaccagcc gcacggggca gatgtatgac catgaccatt attattacga
     6601 caaagcgggc aacctgctga cggatgagta tcagggagcg gtgatggata accgcctgcc
     6661 gggttacggg cgtgaccgct accgctataa cgaatggggc gaactgacag agcgcaggga
     6721 ccagcagctg gagtggaacg cgcagggcca gttaacgcgg gtcatcagca gcaacagcga
     6781 gacgcggtat cagtacgatg cgctgggcag gcgcatcagc aaggcgacca gcaatctcca
     6841 taccgaccgg ggagaacgaa gccggacaac gtttgtgtgg gaagggttcc ggctgttaca
     6901 ggagacaacg tggcagggaa agcggacata cctgtacgat gcggaacagc cgtacacgcc
     6961 ggtggctgcc atcacggggc gcggagagag ccagaaaatc tggtattacc acacggacct
     7021 gacgggcacg gtacacgagg taaccgcgcc ggacgggacg ctggtgtggg cgggctacca
     7081 ggcgggattt ggtgaaaacc ggggcgacat cagcaacagc ggggcgtact ttgagcagcc
     7141 gcagcggctg ccggggcagt actttgacga agaaacaggg ctgcattaca atctgttcag
     7201 gtattatgcg ccggagtgcg ggaggttcgt aagccaggac ccgataggat taaacggtgg
     7261 gttgaactta tatgcttatg cgcctaatcc gttaggttgg atcgatccgc taggactgaa
     7321 ttctttagga atagattcaa ataatatatt tagaggagat ggtaactata aaggtggcgg
     7381 aattggacag ccaaagagtg gcacaatatc agcacaggac attattgatc atctctaaga
     7441 aaaaggtgat gggcgtttaa catcttttag tactaaaaaa acatctagtt ccaactctcg
     7501 tggtgcagat tttttctcaa aaaaacatgt cttcaaagtg gcagtttctg atctcgatga
     7561 tttagttaaa aataatcaga tcactgtttt aacacaagaa aaagtaaaag caatatttgg
     7621 tttagaatca agcaagaaaa ttagaaaaga acttggtaat attcttcaga atatggctag
     7681 gaataatgag gtgttagttc atgggcaaat tcttggaaat aaagttagcc gatgtaatta
     7741 gttagtggag aggaagttgt ggacgaaatt agaataagaa ttgctggtaa tacggctgcg
     7801 ccttgtttcc atgtgctaaa aaataaggga tataaagtaa caatggaaac tcatgcatta
     7861 tctgacgatt gtttagattg tgtatatgat tggtatgcgg aaaaaaatgg acaatttttt
     7921 agtgcaacga gtccagaaga gttacttgga ttagttgcaa tgtgggaagt tcgtggtaat
     7981 gactggagag ctaatttgaa tgagataaat acttatcaaa aagccataga agaatccccg
     8041 ttttttgata aaaacggcaa tgaaatagag tctgactaaa ttttattgat atagcaacga
     8101 taataaccgg aaatttcttc cagatctttc cggcttttta ttatttaact ttcgttcagc
     8161 acactaaaca tcccgtctcg catcccctta actggctgtt taacctgata aagttccctg
     8221 cgcagttcct gtacttcgtt gttatcccaa gcaggatcaa acatccctcc gagatcttca
     8281 cggtaacgcc cgtgccgatc tcaaatcccg cttttctcag ccatttggcg ctgattttga
     8341 tcaccggcgt gtcgctgcgg ttgggcagat aacccacggt acagcgacgc tgtgttttgg
     8401 aaatggtgac ttctggcgta atattgtgtt taagttaggg gctttcgtaa tctagcccag
     8461 cccccggccc taaacacaac ccccactcac cacaacctaa actcatccgc atcccgccat
     8521 gccggaaact tttctctata ttcccgcaat gccaccatcg acagctccgc atcaatacgc
     8581 gttgccttat gcgcgtcggc ggtagcgata atctcgcctt gcggattaat cacccggctg
     8641 tcaccgcgat aatggcagcc gttgccatcg ctgccgacgc gattgcatcc cgccacatac
     8701 gcctgattct caatcgcgcg ggccgtcagc aatgcctgcc agtgcagaga gcgcggagct
     8761 ggccagttgg cgacgtacag ggcgaggtca taatcgttga gattgcgcga ccacactgga
     8821 aaacgtaagt cgtagcacac cagcggcaaa atacgccagc cgcgccattc cacaatcact
     8881 cgcgcattgc ccgctttata atgcagatgc tcatctgcca tgcggaacag atgacgctta
     8941 tcataaaaat gtaccgtgcc gcccggctca accagcaaaa agcggttaac cgaaccagac
     9001 tccgtttgta atgcaacact gcctgcaatc agcgcattgc actgctgcgc cttagctgtc
     9061 atccagttca ctacgtcatc ttgtgcaagc gacgaagctg ccgcttccat ggcaaagccg
     9121 ctggtaaaca tctccggtag aacgatcaca tcgcgcccgg taataccttc cagttgacga
     9181 tcaaaatggc gcaggttggc aggaccatcc acccacacca acggttgctg caaaagcgta
     9241 atcttcaaac taggcacggt gtacaactcc tttatgcgaa gggttttata actttaacac
     9301 cttatcaggc agttgcctta gcgcagaata aattgataac aaatgctgat attggaaata
     9361 tctgatttgc aaattatcgt gttatcgcta ggctttagga ggttaataac atgggcagga
     9421 taagctcggg aggaatgatg tttaaggcaa taacgacagt cgccgcactg gtcatcgcca
     9481 ccagtgcaat ggcgcaggat gatttaacca ttagcagcct tgcaaagggc gaaaccacca
     9541 aagctgcgtt taatcagatg gtgcaagggc ataagctacc tgcatgggtg atgaaaggcg
     9601 gtacttatac tcccgcacaa accgtaacgt tgggagatga gacgtatcag gtgatgagcg
     9661 cgtgcaaacc gcatgactgt ggctcgcaac gtatcgctgt gatgtggtcc gagaaatcta
     9721 atcagatgac ggggctgttc tcggctattg atgagaaaac gtcgcaagag aaactcacct
     9781 ggctgaatgt gaacgatgcg ctttcgattg atggtaaaac ggtgctgttc gcggcgttga
     9841 ccggcagcct ggaaaaccat ccggatggct ttaattttaa ataattagcg gataaagaaa
     9901 cggagccttt cggctccgtt attcaattac gcggcttcaa ctttccgcac tttctccggc
     9961 aactttaccg gcttcgtcgc cagctcttcc ggatcaaagt catcaacgtt aatactgcgc
    10021 agacggcttt cttccgcttt caccagaata gcggcttcat ctttatcaat cagccccttc
    10081 gtcagcgcgt tgtgcgccag ttcatccaga cgggtaaatg gcagattttt acccagctct
    10141 ttacagatcc gctgatgaat cgggtcggcg gcaatcacat ccaccaacgc ctcttccagt
    10201 aaaccaaccg ggttatgctc gcttggcgtc aggtactgac cgcgaccaat acgtgaacga
    10261 gtggcgttcg gcacttgtaa aatcttcgcc attttatgat ccagcttgtc agacggtgcc
    10321 agataatgac gtccggtcgg gaagatcacc acattcagca gcccggcaac cacgcggttc
    10381 gggaagtttt gcagtaaatt gtccattgcc tgttctgcct gatacagcgc atcctgtacg
    10441 ccccagtgca ccagcggcag atcggcttca ttacggcctt cgtcgtcata acgcttcaga
    10501 acggcagagg cgaggtaaag ctggcttaaa atatccccca gacgtgccga gatacgctca
    10561 cgacgtttca ggctgccgcc cagcagtgcc atcgagacat cagaaagcag ggcgaggttg
    10621 gcgctcaggc ggttcaggtg ctgatagtag cgtttagtgg catcgccggt tggcgtgctg
    10681 ctggttaaac cgcgcgtcag gcccagccag aagctgcgaa ctttgttgct accgacgtga
    10741 ccgatatgtt tgaacaacag tttatcgaac gcgttgacgt cattgttctt cgccgcttcc
    10801 atctcttcca gcacgtacgg atggcaacga atcgctcctt gtccgaagat catcatgctg
    10861 cgggtcagaa tgttagcccc ttcaacggtg atggcaatcg gtgcgccctg gtaagcacgc
    10921 gccaggaagt tgctttgccc gagcataatg cctttaccgc cggtaatatc catcgcatca
    10981 ataatcgact gctgcccgcg gtgggtacag tgatacttaa cgatagccga cagcacggca
    11041 ggtttttcgc cgagcataat gccgtaggta atcagcgatg ccgcagcatc cattacataa
    11101 gcgttcccgg caatacgcgc cagcggctct tcaatccctt ccatcttacc gatagagatt
    11161 ttgaactgac ggcgaatgtg agaatacgcg cccgttgcca gcgctaccga tttcacgccg
    11221 ccggttgagt tggaaggcag ggtgatgccg cggcctaccg agaggcactc caccagcatc
    11281 cgccagcctt gcccggccat tttcggcccg ccgatgatgt aatcgatcgg cacgaagaca
    11341 tctttaccgc gcgtcggtcc gttctggaac ggtacgttca gcgggaagtg gcgacgacca
    11401 atttccacgc ccggcgtggt ggttgggatc agcgcacagg taatgcctaa atcttctgca
    11461 ccgccgagta atttttccgg gtcggagagt ttaaacgcca gcccaagcac ggtcgcaatc
    11521 ggtgccagcg taatgtagcg tttgttccag gtcagacgca tccccagcac ctgctggccc
    11581 tgccattcgc ccatgcagac aatcccggtg tccggaatcg cgcccgcatc ggaacccgct
    11641 tccgggctgg tcagtgcaaa gcaggggatc tcctgaccac gcgccagacg cggcagatag
    11701 tgatctttct gctcgtcagt gccgtaatgt tgcaacagtt cgcccgggcc taatgagttt
    11761 ggcacgccga cggtaatcgc caggatcccg ctcacgccag agagtttttg cagcacacga
    11821 gactgggcat aagctgagaa ctccagtccg ccgtactctt ttttgataat catcgcgaag
    11881 aagcgatgct ctttaaggta cgcccacaac tccggtggca gatccgccag ctcatgagta
    11941 atctggaaat cattcgccat ccggcaggct tcttctaccg ggccgtcgag aaacgcttgc
    12001 tcttcagcgg tcaggcgcgg ctgcggatag ttatgcagct ttttccagtc cggcttgccc
    12061 tggaacaagt cgccctccca ccaggtggtg cccgcatcaa tcgcttcttt ctcagtgcgc
    12121 gacatcggcg gcatcacctt acggaaaccg cgaaataccg gcgcggaaat catcgactta
    12181 cgcataggcg caaagttaaa tggcacgagg ataatggtca gaggcaccag tacccacgcc
    12241 gaccacagac cagcaacgcc gagggcggct gtccaggcga gcaaaatcag actgctgata
    12301 aataagctca cgcggtgata gaacaacgcg ccgagcagga caaccgtagc gagaatactc
    12361 aaaatcatca taacgaaaag ccccttactt gtaggaggtc tgaccacttg tgatgatatg
    12421 gttgtagtgg atgtaaaaac atttagcaat gtgtttacaa tataattaca acaaagctca
    12481 cattgttgct gtttttatcc gcacttcagg tcaaaaagtc ctggtcatag cacctgcccg
    12541 tacttctcgc ttttggcggt atccggtaca ctgcattttg tctattacat ttatgctgaa
    12601 ggatatcctc atgtaccagg atcttattcg taacgaactg aacgaagcgg cggaaacgct
    12661 ggctaacttt ttaaaagatg acgccaatat tcacgccatt cagcgcgcgg cggtcctgtt
    12721 agcagacagc tttaaagccg gtggcaaagt gctttcctgc ggcaacggcg gttcccattg
    12781 cgacgctatg cactttgccg aagagttgac cggtcgctac cgtgaaaacc gtccgggcta
    12841 cccggcgatt gctatttctg acgttagtca tatttcctgc gtcggtaatg atttcggttt
    12901 caatgatatt ttctcccgct acgttgaagc ggtaggtcgc gaaggcgatg tactgctggg
    12961 gatctccacc tccggtaact ctgcaaacgt gatcaaagcg atcgcagcgg cgcgtgagaa
    13021 ggggatgaaa gtgatcaccc tgaccggtaa agacggcggt aaaatggctg gcacggcgga
    13081 tatcgaaatt cgcgttccgc actttggtta tgccgaccgc attcaggaga ttcacattaa
    13141 agtgatccat atcctgatcc agttaattga aaaagagatg gttaagtaag tctggcgtag
    13201 gccggataag gcgtttacgc cgcatccggc acttgtgcgc tgatgcctga tgcgatgctg
    13261 acgcgtctta tcatgcctac aaatctgtac ccgaaccgta ggccgaataa ttcgtttacg
    13321 ccgcatccga cctgaaaatt cttaaatcaa tcttcgccgg ggtccatgcg ctcccgctgt
    13381 tgtggaggtt acccatgtgc gaattgctcg ggatgagcgc caacgtccct accgatatct
    13441 gctttagttt caccggactt gtacagcgtg gtggtggaac cgggccacat aaagatggct
    13501 ggggcattac cttttacgaa ggtaaaggct gtcgcacatt taaagatcca caacccagct
    13561 ttaattcccc catcgccaaa cttgtccagg actacccgat aaaatcctgt tcggtggtgg
    13621 ctcatattcg ccaggctaat cggggcgagg tggcgctgga aaatactcac ccatttaccc
    13681 gcgagttatg ggggcgtaac tggacttatg cccataacgg acaactgacg ggctacaaat
    13741 cactggaaac cggcaacttc cgcccggtcg gtgaaaccga cagcgaaaaa gccttttgct
    13801 ggctgctgca taaattaacg cagcgttacc cgcgcacgcc gggcaacatg gcggcagtgt
    13861 ttaaatatat cgcctcactg gcggatgaac tgcgggcgaa gggcgttttc aacatgctgc
    13921 tttcggacgg gcgctatgta atggcgtatt gctcgactaa tttacactgg atcacccgcc
    13981 gcgcgccgtt tggcgtggca acgttgctgg atcaggatgt ggaaatcgac ttcagctcgc
    14041 agaccacacc gaatgatgtg gtcacggtga ttgcgacaca gccgctgacg ggcaatgaaa
    14101 cctggcaaaa aattatgcca ggcgaatggc gcttattttg cctcggggag cgtgtagttt
    14161 gatgccagtt gtggctgcac cacttcgtgg cttaacggct tgctgaccac gtaacggcca
    14221 ttggccacag aaacggtcgg tggcttacgg gtttgctcaa agtagtcgta gcccggcttc
    14281 agctgctccc agaagtcctt aaagttggaa tatttatggc gcttcatatt ggcgtcggtc
    14341 atgcggaatg ggtaaatact cacctgcacg ctcggctgac caaacaccag cgcaccagta
    14401 acgaactgga agatctcatc aataccctga ttggtcattg cgtagcagcc gatggataca
    14461 caatcgccgt ggatcatcag gtatttccct tcataaccat gcgcacggtc ataagcattg
    14521 gggaatccaa tattaatcgc tttgtagtaa cggctgtctg gttttaactg attacgctgg
    14581 acgctataaa acccttccgg gcttttgaaa tcgccctgac gctgttttgg ccctaagccg
    14641 ccggaatatt tacagatttt ataactgtcg agcagttgat attgctcgcc cattttgacg
    14701 taaagatcga gcgtacgttc ttccttgaag atctggatgt agacagggga tcccatcaac
    14761 tgctgcttat actctttgct cactggcgtg gtcgagctac tgctgcccag caaaccggca
    14821 aacgaaacgc acgggatcaa caacatcgca agaattaatg cgattttacg catactgctt
    14881 attccttgat aaaacggtta cacacgccag gacggcaaaa tggatcccaa atcggaatag
    14941 tctggatttg gaaggctcac attatcacca aaagagtttt acgcaagcct gtcggcgctg
    15001 ggtttacaaa tttagcacag gggcagtttt caatagtccg aaaagcgcat gtatcattta
    15061 tttcctaata ttatgaaatc tcaatagtaa tatgcaaata agcaattaat aatcccgttt
    15121 actcctccgg tcttttaaaa ttctcacctt cattcagccg gtttatgtct ttcatgtctt
    15181 cgttcttgct cggcagattt ttgcatccag gcgtgttttc gttatgcgtt ttgctccctt
    15241 tactcgccag cgccaccaca accgggagca gataaaacag catccagaat ggtttcctgc
    15301 gccgctcaag gcgagtgaca gacgctggca ggcgctggcg gaaaacaacc actttttaag
    15361 cagcgatcac ctgcataaca tcaccgaagt ggcgattcac cgcctggagc agcagcttgg
    15421 caagccttac gtctggggcg gcacgcggcc cgatcaaggc ttcgattgta gtgggttagt
    15481 tttttatgcc tacaacaaga tccttgaggc gaaactcccg cgcacggcca atgagatgta
    15541 ccactaccgt cgggcaacga ttgtggcgaa taacgacctg cgccggggag atttgctatt
    15601 tttccatatc cacagccgcg agattgccga ccatatgggg gtgtatctgg gcgacggtca
    15661 gtttattgag tcactgcgca ccggggaaac cattcgggta agccggttag ccgaaccttt
    15721 ctggcaggac cattttttag gcgcgcgcag gattttgacg gaagagatga ttttgtaggc
    15781 cggataaggc gtttacgccg catccggcgg ttgtgcacgg atgcctgatg cgacgctgac
    15841 gcgtcttatc atgcctacaa atctgtgcgt ttttgcgagc cgctttcccg atataacaca
    15901 gcgtcattgt cggcctttcc ttttcctctt accgttgaga aaaaggagtc gctatgtcgg
    15961 aatatcgtcg ttattacatc aaggggggaa catggttttt cacggtaaat ttacgcaatc
    16021 gtcgaagcca tcttctgacc acccagttcc agacgctccg taacgccatt attaacgtta
    16081 agcgagacag accttttgaa attaacgcct gggtcgtttt gccggagcat atgcattgca
    16141 tatggacatt acccgaaagc gatgatgatt tttcctctcg ctggcgggag attaaaaagc
    16201 agttcaccca tgcttgcgga ttgaaaaata tctggcaacc gcgtttttgg gagcatgcca
    16261 ttcgcaatac aaaagattat cgccatcatg ttgattatat ttatataaat ccagagaaac
    16321 atggctgggt aaagcaggtg agtgattggc cattctcaac gttccatcgc gatgtcgcgc
    16381 gagggttata tcccatcgat tgggcgggag acataacgga tttaagtgcc ggggagcgta
    16441 tcattttata actgtgcgct ggtgccggat gcgcttacgc ttatccggcc tacaaatcgt
    16501 gctttaacgg taggccggat aaggcgttca cgccgcatcc ggcagttatg cgcagatgcc
    16561 tgatgcgacg ctggcgcgtc ttatcatgcc taaaaacttg tgcctgaacg gtaggccgga
    16621 taaggcgttt acgccgcatc cggcaattgt gcaccgatgc cggattctta catcaacgcc
    16681 cccataatct tcaactccag ctcatccggc acttcgttat acgacagcac atgcagcccc
    16741 ggcgcaaaca accttgcata acgcggcagc aaagggcgta gctgcggcgg caccagcagc
    16801 accggatctt tccccgccgc tttcatctgc tctttcacct gtggcatcgt gctctggaac
    16861 tggttgagca tattcgggtc aaccggcacg ctgtcgagca tcactttgcc ggcctgttgc
    16921 gcctgattca ctacgttggt cagcagattt tccagctcat tattcagcgt atacaccgtc
    16981 agctcctgct tgcgaacgaa cggatgagta atgctgcgcc gcagcgccag gcgcacatcg
    17041 gccgccagca gaatatgatc tttagtcacc gtgctactgg cgaccagcac ggtggcgatg
    17101 gtgacgatat cgcgcaggga aacgccttcg gtcagcagcg cccggtacac tttcagcaat
    17161 tggctgtaat tgagcgccgc gcttaaatcc tccgccaggc gcggtgccat cgacgacaaa
    17221 cggttatgca actgcgtgat gtcatcatag ttaaacaaat caggaatata gctgcgcaca
    17281 atcttgttca catgggtggc aatcacactg gcgctgtcga tcacctgata ccccatattc
    17341 agagccttcg ctttctgcgc aggctggatc caggtgaccg gtatcccgta cgccgggtcg
    17401 ttccccagca cgccgtcaat ctcgccgtag gtttcgctgg agggcagcgc catcagttta
    17461 tccgccggaa tatccgcttc atcagcctca atgccgttga tgaaaatagc gtactggctg
    17521 ggcttgaggc ggaagttttc ccgaatgcgg atctccggca gcagcacgcc gttaccgtcg
    17581 gaaatcacct gccgaacgcc gcgaatccgc tgggtgagcg ggttgccctg cgctttgtcc
    17641 accagcgcca ccagcttgta gccgaggctt aagctgatgg gctcgatcag cggaatagtt
    17701 tcccaactga cctgttgttc gctggtttca gtgatggtgc gggtcagcgt ttcgaggctt
    17761 ttctcttccg cctccgccgc ctgcgggcgt ttgctcatcc gccagccggt aaagccaagc
    17821 agggcgctga acagcaaaaa cggcagatgc ggcatccccg gcaccaccgc cagcacgaac
    17881 atgatcccgg tggcggtgta gagcaccgac gggcttgcca gcaactggtg gcgcacgtca
    17941 tgggcgatat cgccgttgtc gctgacgcgg gtgacgataa tcgccgccgc ggtggagagc
    18001 agcagggaag ggatctgcgc caccaggccg tcgccgatgg tcatcagcac gtattgctgg
    18061 aaggcggcgt cggcgctcag gttgtatttg aagatgccga tacagacgcc gccgctcagg
    18121 ttgatcgcca gaatcatcat cccggcgatg gcgtctccgc gtacaaactt cgatgccccg
    18181 tccatcgcgc cgtagaaatc ggcctcgctg gcaacatctt tacgccgggt ttgcgcctgc
    18241 gcctggttga tcaatccggc gttaagatcg gcgtcaatcg ccatctgttt gccgggcatc
    18301 gcgtccaggg taaagcgggc agaaacctcg gaaatacgct cggcctcttt ggtgacgacg
    18361 ataaagttga ttgtcaacag cgtcagtaaa tccgagcgtg aaagcattat cgccgcgctg
    18421 cacgggcagt cgatttttaa cggtggtggg ctgtcgccgt taaataaaat cagcccgtct
    18481 catccgccaa aacctgccac cgtggcggtg ccggaagaga cggagaaaaa agcgcgtgat
    18541 gtgaatgaga aaacggcgct gctgaagaag aaatccgcca ccgagcttgg cgagttggca
    18601 accagtatca acaccattgc ccgtgatgcg catatggaag cgaatctgga gatggagatt
    18661 gttccccagg gattacgcgt gctgattaaa gacgaccaga atcgcaatat gttcgaacgc
    18721 ggcagcgcgc agattatgcc gttctttaaa actttgctgg tggagctcgc gccagtgttc
    18781 gactcgctct ataacaaaat tattattacc gggcataccg atgcgatggc ctacaaaaac
    18841 aatatctaca acaactggaa cctttcgggc gatcgcgcgc tttcagctcg tcgggtgctg
    18901 gaagaggccg gaatgccgga agataaagtg atgcaggtaa gcgcaatggc ggaccagatg
    18961 ctgctggacg cgaagaatcc gcaaagcgcg ggcaatcggc gtattgagat tatggtgctg
    19021 accaaaagtg cgtccgatac gctgtatcaa tactttggtc agcatgggga taaagtggtg
    19081 cagccgctgg tgcaaaagct ggataagcag caggtgcttt cgcagcgaat gcgctaaatg
    19141 ctgaatcttt acgcatttct caaaacctga aatcactgta tactttacca gtgttgagag
    19201 gtgagcaatg cgtaaaatca ttcatgtgga tatggactgc tttttcgcag cggtggagat
    19261 gcgcgacaat cccgctctgc gcgatatccc tattgctatt ggcggcagcc gcgaacgtcg
    19321 gggggtgatc agcaccgcca attatcccgc gcgtaaattt ggcgtacgta gcgctatgcc
    19381 tactgggatg gcgctcaaat tatgcccaca tctcaccttg cttccggggc gctttgacgc
    19441 ctacaaagaa gcctcaaatc atatccgtga aatcttctcg cgctacacct cgcgcattga
    19501 accgttgtca ctggatgagg cttatctcga tgtcaccgat agcgtccatt gccacggttc
    19561 tgcgaccctc atcgcccagg aaatccgcca gacgatcttc aacgagctgc aactgacggc
    19621 atctgcgggc gtggcaccag taaagtttct cgccaaaatc gcctccgaca tgaataaacc
    19681 caacggtcag tttgtgatta cgccggcaga agttccggca tttttacaaa ccttaccact
    19741 ggaaaaaatc cccggcgtcg gcaaagtctc ggcggcaaaa ctggaagcga tggggctgcg
    19801 gacctgcggt gatgtacaaa agtgtgatct ggtgattctg cttaaacgct ttggcaaatt
    19861 tggccgcatt ttgtgggagc gtagtcaggg gattgacgag cgcgacgtta acagcgaacg
    19921 gttacgaaaa tccgtcggcg tggaacgcac gatggcggaa gatattcatc actggtctga
    19981 atgtgaagcg attatcgagc ggctgtatcc ggaacttgaa cgccgtctgg caaaggtaaa
    20041 acctgattta ctgattgctc gccagggggt gaaattaaag ttcgacgatt ttcagcagac
    20101 cacccaggag cacgtctggc cgcggctgaa taaagctgac ttaatcgcca ccgcgcgtaa
    20161 aacctgggat gaacgccgcg gcgggcgcgg tgtgcgtctg gtggggctgc atgtgacgtt
    20221 gcttgacccg caaatggaaa gacaactggt gctgggatta tgatgtatac tattatgtat
    20281 attctggtgt gcattattat gagggtatca ctgtatgcat cgaattctcg ctgaaaaatc
    20341 ggtcaatatc actgagttac gtaaaaaccc agctaaatac tttattgatc aaccagttgc
    20401 ggttctttct aataatcgcc ccgcaggata tctcttaagt gccagcgcat tcgaagcgtt
    20461 aatggacatg cttgctgaac aagaggagaa aaagcccata aaggcgcgct ttcgtccaag
    20521 tgctgcaaga ttagaggaaa ttacacgccg cgctaaacaa tatcttaatg atatgacgga
    20581 tgatgatttc aatgacttta aggaataagg atgcgggtat tcaaaacaaa acttattcgc
    20641 ctgcaactta cagcagagga acttgatgcg ttaacggcgg attttatttc ctataagcgt
    20701 gacggtgttt tgccagatat atttggtcgc gatgcactct acgacgactc gtttacctgg
    20761 ccattaatca aatttgagcg agttgctcat attcatctgg caaatgtgaa taatccattt
    20821 ccgccacagt tgcgccaatt cagcagaacg aatgacgaag cgcatttggt atattgtcag
    20881 ggtgcgtttg atgagcaagc atggttgctc attgccattc tgaagcctga acctcataaa
    20941 ctggctcgag ataacaacca aatgcataaa attggaaaaa tggcagaagc gtttcgcatg
    21001 cgtttttgaa tttatattat gaataacata caaataagag attatcagcc tggcgatttt
    21061 cagcaactat gcgctatttt cattagagcg gttacgatga ccgtcagtca gcattattca
    21121 ctacaacaaa ttgccgcctg ggcgcagatt gacgaatctc gctggaagga gaaactcgcg
    21181 aaatcacaag tgcgggttgc agttattaat gcaaaactgg tcggctttat tacctgcgtt
    21241 gaacactata tcgatatgtt atttgttgac cctgaataca cccgccgtgg ggttgccagc
    21301 gctttgttaa aaccttttat taagtctgaa tccgaactta cggtggacgc aagcataacc
    21361 gcaaaaccct tttttgaacg ttatggtttt cagacagtta agcagcagcg cgttgaatgc
    21421 cggggagcgt ggtttattaa tttttatatg cgttataaac cgcaacatta aatccagctt
    21481 gcaatgaaaa taacgcccgc ctagtatgtg cgcgttttct tattccacaa actgcaagga
    21541 ggtaaatcat gacaaatcct ttatcaatga ctctttgcag acctttccag gattaattct
    21601 ttttttcttg ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa
    21661 ttcaagtcgc agcacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg
    21721 gcaattatat tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga
    21781 tcgagagttt agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg
    21841 tagtggggat gccagattta caccccggac gcggctaccc gattggcgca gcgttcttct
    21901 ccgcaggccg tttttaccca gcactggtcg gcaatgatat cggctgtggc atggcgctat
    21961 ggcaaacaga tattctcgct cgcaaataca acgccgataa gtttgaaaag cgattatcag
    22021 ctttggatga cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac
    22081 agcatccgtg gcgcagctcg cttggctcta tcggtggtgg caatcatttc gctgaactgc
    22141 aacaagttga tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc
    22201 atctgcaact gctggttcat agcggctcga gggggctagg gcagtctatt ttacagcggc
    22261 atattgcctc gttttcgcat catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg
    22321 cgcgttttaa tcgccacctg atcgctttgc gcataatgca acaggttaag gccaccggaa
    22381 gtccggttct ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt gatcaacaag
    22441 gggtcttaca tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt attcccggtt
    22501 cacgcggtga ttactccagg ctggttcagc ccgtcaggag tgaggaaaca ttgcattcgc
    22561 tggcgcatgg ggctgggcgt aaatgggggc gcaccgagtg taaagggcgt ctggcagcga
    22621 aatacacagc gacgcaactc tcgcgtactg aacttggcag tcgggtaatt tgtcgcgata
    22681 aacaactcat cttcgaagaa gcgccacaag cctataaatc ggctgaaagt gtggtgcaat
    22741 gtcagggcag gccggattaa ttattcctgt cgcgcggctg cgtccggtgc taacgctcaa
    22801 aaacagtgga gggaaaaaag gatgatcttg ctataactct cctcagcgca ggggccggaa
    22861 gaatgttgtc tcgcagtgaa aaaggcgctg gacaggctaa ttaaagaagc tgcccgacaa
    22921 gacgtcgcgg taacggtgct ggaaacagaa acaggccgct actctgacac gctgcgttcg
    22981 gcgctgattt ctctggatgg cgacaacgca tgggcgttaa gcgaaagctg gtgtggcact
    23041 attcagtgga tttgcccgag cccgtatcgc ccacatcacg ggcgtaaaaa ctggtttctg
    23101 ggcattgggc gttttaccgc tgatgagcag gagcaatccg atgcgatccg gtatgagacg
    23161 ctgcgttcgt cggggccggg cggtcaacat gtcaataaaa ccgactcggc ggtacgcgcc
    23221 acgcatttgg catccggtat tagcgtgaag gttcagtcag agcgcagcca gcatgctaac
    23281 aaacggctgg cgcgattgct gattgcctgg aagctggatc agcagcaata ggaaaatagc
    23341 gcggtgctga aatcacagcg gcgaatgttc catcaccaga ttgaacgtgg caacccgcga
    23401 cgaacgttta cagggatggc ttttatcgaa ggataatgaa ggatgaaact gccggaaggc
    23461 gattaaacgc catccggcag caaatcaaat aattacttcg ccggaatttc tttcagcagt
    23521 tcagtcagca gtgtccagta atgacctacg ctttcgatgt gaacttgctc atccggagag
    23581 tgtggaccgg tgatagttgg cccgatagaa accatgtcca tttccggata cggttttttg
    23641 aacagaccac attccaggcc cgcgtggata atctggatgt tcggcgtctt gttgaacagg
    23701 cgctgatagg tttcacgtac cagatgcatc accggagaat tagcgtccgg ctgccagcca
    23761 ggatatgcgc ctttcgcttc ggttttcgcg ccagccagtt tacccagcga atccagcatg
    23821 ctcaccacgt agtctttacc gctgtcgatt agtgaacgga tcaggcagtg aatttctacg
    23881 ttattgtcag tcatggtcac cacaccgacg ttcagggagg tttcgaccac accttttgcc
    23941 acgtcggaat tgcggatcac accgttcggg gtggcgttca gcagacgaat aaaggtatcg
    24001 cgagatttcg caatcagggc agctttatcg ttcgctacag agtccagcaa caaggccaga
    24061 tttttctctt tctctgccag ctcgtttttc aggatctcct gataggtatt caccagagat
    24121 ttcagtgcgt cgactttatc agctgcgaca gcaatggtcg caaaggcttc acgcgggatg
    24181 gcgttacgca gtgtgccgcc gttgaaatcg ataaggcgca ggtccagttc ttctgcatga
    24241 cccgccagga agcgcaccag cagtttgttg gcattaccca ggccaacgtg gatttcaccg
    24301 ccggagtgac cgcctttcag accttttaag gttaacttga aggtttcaaa accagctgga
    24361 accgcttcac gatctaaatg caggttggag gtgaagtcga taccccccgc acaacccatg
    24421 tagatttcac cttcttcttc ggagtcggtg ttaatcagaa tatcagcctg caaccagttg
    24481 ctctgtaagc cgaacgcacc gtccataccg gcttcttcgg tcatggtcag cagcacttcc
    24541 agcgggccgt gaaccacgtt ttcgtcagcc agaaccgcca gtgcagaggc cataccaatg
    24601 ccgttatccg cacccagcgt ggtgccgcgc gctttaaccc attcgccatc aatataaggc
    24661 tggataggat ctttcgtgaa gtcatgcacg gtgtcgttat ttttctgcgg caccatatcg
    24721 aggtgggcct gtaagacgac cggtttacga ttttccatac ctgcggtagc aggttgacga
    24781 atcaggatat tacctacctg atcgcgttcg acatggaaac ctttctcttt tgcccaacca
    24841 acaatgtatt cagcgagttg ctcttcatga taggacgggt gaggaataga acagattttg
    24901 gcaaaaatat cccacagcgg ctgtggagat aattgagaca gttcagacac gttaagtctc
    24961 cttgtcgatc acccgcaaaa cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa
    25021 cacaagacag gcttgcgaga tatgtattga gaataccact ttatcccgcg tcagggagag
    25081 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
    25141 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
    25201 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
    25261 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
    25321 gtggcggtct ggtaccgggg gcgttactgg cgcgtgaact gggtattcgt catgtcgata
    25381 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
    25441 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
    25501 ctgttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
    25561 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggatcgaac
    25621 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
    25681 gcctggcatt gccgggcgtt gttcttttta acttcaggca ggttacaata gtttccagta
    25741 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
    25801 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
    25861 cctgtgcagt cggctcttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
    25921 ctgatgtgga tctggcgcgg tattgaccca cgtgaaatcc tcgacgtcca ggcacgtatt
    25981 gtgatgagcg atgctgaacg taccgacgat gatttatacg ataccgtgat tggctaccgt
    26041 ggtggcaact ggatttatga gtgggccacc caggcgatgg tgtggcaaca aaaagcctgt
    26101 acggaagaag atccgcaact cagtggtcga cactggctgc atgcggctac gttgtacaac
    26161 attgccgcct atcctcatct gaaaggagat gacctggccg agcaagcgca ggctttatca
    26221 aaccgcgcct atgaagaggc cgcccagcgt ctgccgggca cgatgcggca gatggagttt
    26281 accgtacccg gcggtgcgcc catcaccggc tttttgcata tgccgaaagg cgatggcccg
    26341 ttcccgacag tattaatgtg tggtggtctg gatgcgatgc agacggacta ttacagcctg
    26401 tatgaacgtt attttgcgcc gcgcggcatt gcgatgctga ctattgatat gccgtcggtg
    26461 ggcttttctt caaaatggaa gctcacccag gactccagcc tgttgcatca gcacgtctta
    26521 aaggcgctgc ctaacgtacc gtgggtggat cacactcgcg tcgcggcctt tggtttccgt
    26581 ttcggcgcta acgttgccgt gcgtctggca taccttgaat cgccgcgtct gaaagcggtt
    26641 gcctgtcttg gtccggtagt tcataccctg ttgagtgatt ttaagtgcca gcaacaggtg
    26701 ccggaaatgt atcttgacgt tctggcgagt cgtttgggga tgcatgatgc ttccgacgaa
    26761 gcgttacgcg tggagctgaa tcgctattca ttaaaagtgc aaggattgct ggggcgtcgc
    26821 tgcccaacgc caatgttatc aggctactgg aagaacgatc cgttcagccc ggaagaggac
    26881 tcacgcttaa tcacctcatc atctgctgac ggtaaattat tagagatccc atttaacccg
    26941 gtgtatcgga attttgacaa aggtcttcag gaaatcaccg gctggatcga aaaacgcttg
    27001 tgttaaaaat ttgctaaatt ttgccaattt ggtaaaacag ttgcatcaca acaggagata
    27061 gcaatgacgt taccgagtgg acacccgaag agcagattga tcaaaaaatt taccgcacta
    27121 ggcccgtata ttcgtgaagg taagtgcgaa gataatcgat tctttttcga ttgtctggct
    27181 gtatgcgtca acgtgaaacc ggcaccggaa gtgcgtgaat tctggggctg gtggatggag
    27241 ctggaagcgc aggaatcccg ttttacctac agctaccagt ttggtctgtt cgataaagca
    27301 ggcgactgga agagtgttcc ggtaaaagat actgaagtgg ttgaacgact ggagcacacc
    27361 ctgcgtgagt ttcacgagaa gctgcgtgaa ctgctgacga cgctgaatct gaagctggaa
    27421 ccggcgaatg attttcgtga tgaaccggtg aagttaacgg cgtgagtgaa atgtgccgga
    27481 tgcatcacat ccggcaatat tcattaaaac tgatacgtca tcccaaccgc gacagtatca
    27541 tcattattaa tattcagctt attatcgcta tccagttggt tgattttata atcaacaaac
    27601 gctgacatat ttttgttgaa ataatacgta gcaccgacgt cgatataatt gaccagatct
    27661 tcatcaccga tgccctcaat atctttccct ttcgataaga cataacccag cgatggacgc
    27721 agaccaaagt caaactggta ttgagcgacc gcttcaaagt tctgtgtctt attggcaaag
    27781 ccgccagtta ttggcgtcat tttgcgtgtt tcagaataga aagttgccag ataaatatta
    27841 ttggcatcgt atttcagacc tgttgcccat gcttctgcac gcttgcctgt gccacggctt
    27901 tgcaggttct gctcgttggt gcgatctgag ttggtatagg ccccactaat ggcgaaatcg
    27961 ctgccgccaa agtcatatgt caatgacgtg ccgaagccat cgccgttttg ctttttaacg
    28021 tcgcggtttt cgtttttccc ttgatattgc agggttaagt tcaggccatc gataacgccg
    28081 aagaagtcgg tgttccgata cgtcgccaga ccgctggcgc gtttggtcat aaagttgtcg
    28141 gtctgcgccg aggaatcgcc accaaattcc gggaacatat cggtccaggc ttccacgtca
    28201 tacaacgcgc ccaggttacg accataatcg aaagaaccca aatctttata tttcaacccg
    28261 gcaaaagcga gacgcgtttt ttgctgtgca gtatcactct ccgctttatt tccggcaaac
    28321 tccgcttccc accgaccata accagtcagt tgatcgttaa tttgagtttc acctttaaaa
    28381 ccaaaacgga tataactctg gtcgccatct ttactggcgt tatcactcat ataatgcatg
    28441 gctttaactt tgccatagac atccagttta ttaccgtctt tattatatat ttctgcagcc
    28501 tgtacagatg cagatgccac aatgcccatc accactaatg ccagagtgct ctttttcatt
    28561 ttcattcctg attttaatta acgcgcgaat attcagcggg agagccccgt tgaaaacagg
    28621 aaagttttta acctgagatt gttaaagata tattacagat taatgatatt
//
LOCUS       DAZXUL010000048        28419 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZXUL010000048 DAZXUL010000000
VERSION     DAZXUL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28419
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.048"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            563..1333
                     /locus_tag="ACH3L1_002070"
     CDS             563..1333
                     /locus_tag="ACH3L1_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFT4076800.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1324..1797
                     /locus_tag="ACH3L1_002071"
     CDS             1324..1797
                     /locus_tag="ACH3L1_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFT4076801.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1904..2443
                     /gene="dnaT"
                     /locus_tag="ACH3L1_002072"
     CDS             1904..2443
                     /gene="dnaT"
                     /locus_tag="ACH3L1_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFT4076802.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2446..3183
                     /gene="dnaC"
                     /locus_tag="ACH3L1_002073"
     CDS             2446..3183
                     /gene="dnaC"
                     /locus_tag="ACH3L1_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFT4076803.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3232..3726
                     /gene="yjjA"
                     /locus_tag="ACH3L1_002074"
     CDS             3232..3726
                     /gene="yjjA"
                     /locus_tag="ACH3L1_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFT4076804.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3966..6257
                     /gene="opgB"
                     /locus_tag="ACH3L1_002075"
     CDS             3966..6257
                     /gene="opgB"
                     /locus_tag="ACH3L1_002075"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFT4076805.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="ACH3L1_002076"
                     /pseudo
     CDS             complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="ACH3L1_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7556..8470
                     /locus_tag="ACH3L1_002077"
     CDS             7556..8470
                     /locus_tag="ACH3L1_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFT4076806.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8685..10046
                     /locus_tag="ACH3L1_002078"
     CDS             8685..10046
                     /locus_tag="ACH3L1_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4076807.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="ACH3L1_002079"
     CDS             complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="ACH3L1_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFT4076808.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAVALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="ACH3L1_002080"
     CDS             complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="ACH3L1_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFT4076809.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12596..13885
                     /gene="hpaG"
                     /locus_tag="ACH3L1_002081"
     CDS             12596..13885
                     /gene="hpaG"
                     /locus_tag="ACH3L1_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFT4076810.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13882..15348
                     /gene="hpaE"
                     /locus_tag="ACH3L1_002082"
     CDS             13882..15348
                     /gene="hpaE"
                     /locus_tag="ACH3L1_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFT4076811.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEGLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15350..16201
                     /gene="hpaD"
                     /locus_tag="ACH3L1_002083"
     CDS             15350..16201
                     /gene="hpaD"
                     /locus_tag="ACH3L1_002083"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFT4076812.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16211..16591
                     /locus_tag="ACH3L1_002084"
     CDS             16211..16591
                     /locus_tag="ACH3L1_002084"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFT4076813.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16659..17462
                     /gene="hpaH"
                     /locus_tag="ACH3L1_002085"
     CDS             16659..17462
                     /gene="hpaH"
                     /locus_tag="ACH3L1_002085"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFT4076814.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17473..18276
                     /gene="hpaI"
                     /locus_tag="ACH3L1_002086"
     CDS             17473..18276
                     /gene="hpaI"
                     /locus_tag="ACH3L1_002086"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFT4076815.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18298..19653
                     /gene="hpaX"
                     /locus_tag="ACH3L1_002087"
     CDS             18298..19653
                     /gene="hpaX"
                     /locus_tag="ACH3L1_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFT4076816.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19663..20553
                     /gene="hpaA"
                     /locus_tag="ACH3L1_002088"
     CDS             19663..20553
                     /gene="hpaA"
                     /locus_tag="ACH3L1_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFT4076817.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20580..21517
                     /locus_tag="ACH3L1_002089"
                     /pseudo
     CDS             20580..21517
                     /locus_tag="ACH3L1_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21744..23302
                     /gene="hpaB"
                     /locus_tag="ACH3L1_002090"
                     /pseudo
     CDS             21744..23302
                     /gene="hpaB"
                     /locus_tag="ACH3L1_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23320..23832
                     /locus_tag="ACH3L1_002091"
     CDS             23320..23832
                     /locus_tag="ACH3L1_002091"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFT4076818.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24225..26375
                     /gene="btsT"
                     /locus_tag="ACH3L1_002092"
     CDS             24225..26375
                     /gene="btsT"
                     /locus_tag="ACH3L1_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFT4076819.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26425..26628
                     /locus_tag="ACH3L1_002093"
     CDS             26425..26628
                     /locus_tag="ACH3L1_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFT4076820.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26639..27595
                     /gene="yjiA"
                     /locus_tag="ACH3L1_002094"
     CDS             26639..27595
                     /gene="yjiA"
                     /locus_tag="ACH3L1_002094"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFT4076821.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27664..>28419)
                     /locus_tag="ACH3L1_002095"
     CDS             complement(27664..>28419)
                     /locus_tag="ACH3L1_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFT4076822.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6955 a         7426 c         7127 g         6911 t
ORIGIN      
        1 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
       61 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
      121 atgataacct ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttagaaa
      181 tacttaatga aaaacagaaa tctcattcat ttaatttata attaaatgat atctcttccc
      241 gcagaatgcg ggaatcacac aatcaatatt tattcaataa aatctgatga ttttaacttg
      301 ttaacactaa aaaacgcgcc cagctatcta ttatcctgac ccgtaaaggc taaacaataa
      361 attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      421 ttatcaccga gcttgtccca ataaccagga ttaatcagcg caattttgat cttaacctac
      481 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      541 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg
      601 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      661 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa
      721 cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      781 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      841 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc aaccattacg
      901 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      961 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta
     1021 tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc
     1081 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
     1141 caccattgcg atggccgcca gcgttctgct gctagtgccg ggctttccgt tgattaatgc
     1201 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
     1261 tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg
     1321 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1381 cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg
     1441 ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt
     1501 gagtggtcaa cctttatggc ttctatgctg gtcggtacca ttggtattca atggtcgcgc
     1561 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1621 atatcggctt ataccgcaat gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa
     1681 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1741 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1801 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1861 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1921 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1981 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     2041 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga
     2101 tgtcgctctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     2161 ggggaaattc gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc
     2221 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     2281 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     2341 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2401 cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2461 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2521 ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2581 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2641 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2701 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2761 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2821 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     2881 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2941 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     3001 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     3061 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     3121 agtttatggg tgatcttcaa ctgggatagc taccgtagcc gggtaacggg taaagagtat
     3181 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     3241 actattaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     3301 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3361 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga
     3421 aaccaagcct taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg
     3481 aagcaaaagc tggcttcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3541 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg tattcagggt
     3601 ttgctgaaaa caaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     3661 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     3721 tcctgaaccg cgaaatgtta cccggctgac gcggctctct cctgcccttt tccctgctcc
     3781 tggacggttt tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa
     3841 ctaattccta acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca
     3901 ttacattgtg cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc
     3961 gcccattgtc agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat
     4021 ggaaagcggg acgtaacacc tggtggtttg cagccacgtt aacggtgctg gggctatttg
     4081 tcgttttaaa tatcaccctg tttgccagcg actattttac tggcgatggt attaacgacg
     4141 cggttctcta taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc
     4201 cgggtatcgg cattgtgctg gggctgacgg cggtgttcgg tgcgctgggc tggatcctgc
     4261 gccgtcgtcg tcatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc
     4321 tgggttcggt ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt
     4381 cacgcgatgg cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg
     4441 acccgaaact caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata
     4501 acgaagcttt cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt
     4561 tcagccacac gcagcagctg ccaggaacgg attacacgat tgcgggcatg gtggcttctc
     4621 agtgcggcat accgctgttc gccccctttg aaggcaacgc ctccgcctct gtctccagct
     4681 tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt
     4741 tcgtgcaggg cgcaaatctg cgttttgccg gtaaagatgt gttcctgaaa tcgcacggct
     4801 tcgaccactt atacggctca gaagagctga aaagcgtggt agccgacccg cactatcgca
     4861 acgactgggg attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc
     4921 tttcccgctc aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg
     4981 atggttttat ctcgcgcacc tgtaaccgca aaaaatatga ttttgacggt aagccgaatc
     5041 agtcattcag cgcggtaagt tgcagccagg agaatatcgc gacgtttatc aacaaaatca
     5101 aagcgtcacc gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat ttagcgatga
     5161 acaacacggc gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc
     5221 gtggcgacaa gccgcagcaa aagacgctgg cagtgaagcg taacacgatg gataacggcg
     5281 cgacggtgct ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat
     5341 ccgggcagtc gatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc
     5401 cagatatcat ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc
     5461 agaaaaacat gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt
     5521 cagacaaacg cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc
     5581 tggccgattt cgctccacgc gacaatttcg tctgggttga cagttgctac aagatggcac
     5641 aactctgggc tccggaactg gcactttcca ccgactggtg tgtctcgcag gggcagcttg
     5701 gcggtcagca aattgttcag catgttgaca aaacaacatg gaagagcaag acggcattta
     5761 aagatacggt catcgacatg gcgcgttaca aaggcaatgt cgatacgctg aagattgttg
     5821 ataacgatat tcgctacaaa gccgacagtt tcatctttaa cgtcgccggt gcgccagaag
     5881 aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg gggccgctgg tccaacgcgc
     5941 agctgggcga tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa tttgacctgg
     6001 tgattaccgc caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca
     6061 atgaagaaca aactcttgtg ctgggcaatg aagtgaccac caccacactg catttcgata
     6121 acccaaccga tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag
     6181 ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagtgg
     6241 tagaacgtga agggtaattc ctgactgatg tgtaggtcgc atagggtgcg acctacacgc
     6301 cttctccggc ttacaacaca ttattaagaa acaaaaaaaa cagcgcccaa cacttccccg
     6361 gacgctgcat ggcaacttta ataatctcgc cttatcagaa agtaattaca cccttaatca
     6421 actcacgatt gttaatcaca tcgcgctcgt aggtttctgc cagcgtggcg aacggatagc
     6481 gatgggttaa catcatgtcg gcagtgattt tcccttccgc catcagtcga ccgactttag
     6541 cgaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt
     6601 ccggatcgga gaactgcaac tcacctttaa acagaccgac aaataccacc gtgccgccgt
     6661 gacgaatcag attcacggta ttattcatcg catgttgatt acctgtcgcg tcgatcactt
     6721 tctgcgccag cgaaccacca aactgcgccc gcagctgcgc gtcaaaatct tcggctgacg
     6781 ggtccagcac gggcaactcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat
     6841 ccgccaccac cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg
     6901 gccccgcccc gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat
     6961 gagcgctaat agcgaaaggt tcaatcaatg cggccgcctg cgggtcaata ccgtctgcgg
     7021 gcaaaatgtt cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc
     7081 caatgactga aattttttca cagcagttgg tacgcccgct tttacacgcc gggcattgct
     7141 gacaggcaac ataagggatc acagcaactt gctgaccatt tttaagatca gcaatatttt
     7201 tacccagccc aacaatctcc ccacatattt catggcctaa aacccgtgga taactaaaaa
     7261 atggttgatt tccaccctag gcatgaatat cggtaccgca aatcccgaca gacttaattt
     7321 ttattaatgc ttcattgtca cccggaatag gtatctcgcg ttgtttccag actaattctt
     7381 tcggctgctg gcaaattaaa acattcatcg tagacatgat tcggctatcc attttttgtt
     7441 gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg gaaaatctgt tttgtgaaaa
     7501 cacacgcata aaaatgtttt aaatcgggtt ttaatggact cacaaggagg aaaatatgag
     7561 tcgttcacaa aatttacgcc acaatgtgat taaccaggtc attgatgata tggcgcgggg
     7621 tcatatccct tccccacttc catcgcaaag tgcgctggcg gaaatgtaca acatcagccg
     7681 cactacagtg cgtcatattc tcagccaatt acgcgaatgc ggcgtcttaa cgcaggttgg
     7741 aaacgactat gttattgcgc gtaaacctga ccatgatgac ggtttcgcct gtactaccgc
     7801 ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt ttcacgatga taaaccagcg
     7861 tcagttacgc cctagggaaa ctttttctga actgcaactg gctcgggccg cgggtgtcag
     7921 cccggtggtt gtaagagaat atcttttaaa attcggccgt tataatctta ttcaaagcga
     7981 aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc tacgccgagc agctgtttga
     8041 gctacgcgaa atgctcgaaa cacattcact tcagcacttt ctcaacctgc ctgatcatga
     8101 tccccgctgg ctgcaggcga aaaccatgct agaacgtcat cggctactgc gtgacaacat
     8161 cggcaatagc tttcgcatgt tctcgcaact tgacagagat tttcactcct tactgctttc
     8221 agctgccgac aatatctttt ttgatcaatc gcttgagatc atctcagtca tctttcattt
     8281 tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat atcattgccg tcgatgaaca
     8341 tatgaccatt ctcagcgctc tgatttgccg cagtgacctt gatgccaccc ttgcgctccg
     8401 taaccatttg aattcagcta aacaatcaat gattcgatcc attaatgaga acacgcgtta
     8461 tgctcattaa gtacggatta aaaacagcaa actattagcg ggtgcacggt tttatcaaaa
     8521 gataaatacc gggaaattcc cgcttaccta tgctcacaat cagtcgacta aaaatgtttt
     8581 tgtgagcggt agtaaagtcc taaaacttta acctgccgtt aatgtcaacc ttgcgtaatg
     8641 cacaattacg taaacaatta aaattataaa tacaggagtc cggcgtggaa aaagaaaata
     8701 tcaccctcga tccgcgttct tcatttactc catcttcgtc ggcagatatt cccgtgccac
     8761 cagatggatt agttcaacgc agtacccgaa ttaaacgcat tcaaaccacc gccatgttgt
     8821 tattattttt tgcggcggta atcaattatc tcgaccgcag ttcgctgtcg gtagcaaatt
     8881 taacgattcg tgaagaattg ggattaagtg ccaccgaaat cggcgctttg ctctccgtgt
     8941 tttcactcgc ttacggcatt gcgcaacttc cttgcggccc actattggat cgtaaaggcc
     9001 cacgcctgat gctgggactg gggatgttct tctggtcact gttccaggcc atgtctggca
     9061 tggtgcacag ctttacgcag ttcgtgttgg tgcgtatcgg tatggggatt ggtgaagcgc
     9121 cgatgaaccc atgcggtgta aaagtcatta acgactggtt caacatcaaa gagcgcggac
     9181 gcccgatggg cttcttcaac gcagcttcta ccattggcgt tgctgtaagc ccaccgattc
     9241 tggcggcaat gatgctggtg atgggctggc gcgggatgtt tattaccatt ggtgtactgg
     9301 ggatttttct cgccatcggc tggtatatgc tctatcgcaa ccgcgagcac gtagaactga
     9361 ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa tgcccgccga gatccgctca
     9421 attttgccga atggcgcagc ctgttccgta accgcacaat gtggggaatg atgctcggat
     9481 tcagtggcat caactacact gcgtggctgt atctggcctg gcttcctggt tacctgcaaa
     9541 cagcctataa cctggattta aaaagcacag ggttgatggc ggctatccct ttcctgtttg
     9601 gggctgccgg gatgctggtc aacggttacg ttactgactg gctggtcaaa gggggaatgg
     9661 ctccgattaa aagccgtaag atctgcatta ttaccgggat gttctgttct gccgccttta
     9721 cgctgatagt accacaagcg acaacatcca tgatggcggt tctgctgatt ggcatggcac
     9781 tgttctgtat tcactttgcc ggaacatcct gctggggctt gatccacgtc gcagttgctt
     9841 ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc cagcttcatc tgcgcctctt
     9901 tcgcgccgat cattactggt tttattgttg atacaaccca ctcattccgt ctggcactaa
     9961 tcatttgcgg ttgcgtcacc gcagcgggag cactggcgta catcttcctg gttcgccagc
    10021 cgatcaacga cccgcgtaaa gattaaactc cctatgcccg ataacatttt gcttatcggg
    10081 catttttatg gcgattaaaa cgtttcccag ttctccccgc tatctgccac tgccatttta
    10141 cgcggcgtag ctggcgtcac ggtttttacc acagccgatg tttcacgctg ctgttgctgc
    10201 tgttgctgaa tccggaacac tgccacagct tcggtcaggc gactggcctg ctcttccagc
    10261 gcgacggcgg cagcggcaga ttcttccacc agcgcggcgt tctgttgagt tacccggtcc
    10321 atctcagcaa ccgctaagcc aacctgatcg ataccacggc tctgctcatc agaagcagaa
    10381 gctatttcgc ccataatgtc cgtcacgcgg gtcacagcac tgacaatctc cgccattgtt
    10441 tccccggcgc tttcgaccag cgtagagcca acatccactt tccccaccga gtcttcaatc
    10501 aggcttttaa tttcacgaac cgcctgggcg ctacgctggg ccagattacg cacttctccc
    10561 gcaaccaccg caaaaccacg tccttgctca cccgcacgcg ctgcttcaac cgccgcgttc
    10621 aaagccagaa tattggtctg gaaggtaatg ccgtcaatta cgctgataat atcggcgatt
    10681 ttctgcgaac tggtggagat atcgcgcata gtctgcacca cgttatctac cactttgccg
    10741 ccgcgttgcg ccgtttcaga agcacttaat gccagatggc tggcctggcg cgcattctcg
    10801 gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg ctgccgtctc ttccagcgaa
    10861 gcggcctgtt gctcggtgcg cgaagagaga tcattattgc cggtagcgat ttcgctggca
    10921 ccgctataga tggcattggc cccgttgcgc acatcaccga cggtacgcat cagctctccc
    10981 tgcatatggc gcaaactctc tgccagttgc cccatctcat tagagccatc cacctcaatc
    11041 ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt caatcaggcg attcattggc
    11101 gctaccagcg aggctttaat accgaaccag acggcgaaga tgaccgccag tacgacgatc
    11161 atcacgccca ccagaatcca catcgcctgg ctgtaggagg cattgttatc gctgacggcg
    11221 atatcataga gccgatcgtt ttgctccatg taagccacat actgcttctc gaaaccgtcc
    11281 tgatatccct gggtcggctg atcaaagaac tcgttgattt tgcctgcacc taacagttgg
    11341 atcagctccg ccagcgcatt gtgataaata tcgtaattac gtttgatctc tgccgctgcg
    11401 gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat ccgcccagtt tttttccgcc
    11461 tgtttcagcg aaatactggc actctgcatc agctcagcaa cggttgaacc gctaccaata
    11521 ttattctgat ccatcatgta gcggataccc gcgcggttga gggtgttacg cgtctgcaac
    11581 aacgcgaccc agctgccatt cagcgtgggt tgctgctggc gaatggtttg taaaacagtg
    11641 aaattttctt tgtcattctt taaggcatta aaaaacagac cgcctgatgt cagttgtaaa
    11701 aggccaaaaa cggccaaaac cagcagtaag ctggtcacga tcttgatacg ttttaacatg
    11761 gtttctcttt cctgtggacc gatgcaagat tttcggcctg gaaagcaaaa actttatgaa
    11821 aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt tatcagatac taaaaagtta
    11881 ttcattatcg cccgggatgt cttcttgtcg ggaattgccc agagcaataa attcctctaa
    11941 cagatgggtt aactgttgca ttttctcggc agtaaactga gcttcaatct gccgataggc
    12001 ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc ccctctttgg tcagcgagat
    12061 atagagcttc cgctgatcat tgatcggctt taatcgcaac actaaaccgt cgcgttccat
    12121 ccgcgtgagg atcccggtca ggcttgggcg caaaatgcag gcgcgatacg ccagatcgtg
    12181 aaaatccatt gatgggcttt ccgccagaat acgcacgatg cgccactgtt gctcagtcaa
    12241 attatgccgt ttaacgattg ggcgaaaata actcatcgcc gcttctcgcg cctggagcaa
    12301 cgcaatggtt agtgagtcgt gcattatctt tcccctgaaa aattcaaaat cattaatata
    12361 gaaacaatta atcatcacgt taccgggact gagctgcccc cgcaattctt cattttatca
    12421 aaattgatga tattttatta acttactgat atttaatatt ttattatctt taaatgtaaa
    12481 tagtttgtta attagatcac atttatatca cttacttttg ccaaactacg caatcgggaa
    12541 taaaagtaat cattaacata ttaatgatta agatgatcga atccgaggag tggtaatgaa
    12601 aggcactatc ttcgccgtag cgttgaacca tcgcagccaa cttgatgcct ggcaggaagc
    12661 gttccagcaa tctccctaca aagccccgcc taaaactgcg gtctggttta ttaaaccgcg
    12721 caatacggtg attggttgcg gtgaaccgat tccctttcca cagggtgaaa aagtactgag
    12781 cggtgcgact gttgcgctga ttgtggggaa aacggcgacg aaagtacgtg aagaagatgc
    12841 ggcagagtac atcgccggat atgcgctgga taacgacgtc agcctgccgg aagagagctt
    12901 ttaccgtccg gcaatcaaag caaaatgtcg tgatggattc tgccccattg gcgaaaccgt
    12961 ggctctcagc aatgtcgata atctgactat ctataccgag atcaacgggc gtcctgccga
    13021 tcactggaat accgccgatt tacaacgtaa cgccgcacag ctgctgagcg ccctgagcga
    13081 atttgccacg ctgaatccag gcgatgctat tctgctcggc acgccacagg cgcgcgtgga
    13141 aatacagcca agcgatcgcg tgcgtgttct cgcagaaggt ttcccgccgc tggaaaatcc
    13201 ggtagtggac gaacgtgaag tgaccacgcg caagagcttc ccaacgctgc cacacccgca
    13261 cggtacgctg tttgccctcg gcctgaacta cgccgaccac gccagcgaac tggaatttaa
    13321 gccaccggaa gagccgctgg tgttcctgaa agcgccgaat accctcactg gcgacaacca
    13381 gacctccgtg cgcccgaaca atattgaata catgcactac gaagcggagc tggtggtagt
    13441 tattggcaag caggcgcgta acgtcagcga agccgatgcc atggattatg tcgcgggcta
    13501 caccgtgtgt aacgactacg ccattcgcga ctatctggaa aactactacc gccctaacct
    13561 gcgggtcaaa agccgcgacg gactgacgcc gatgctttca accatcgtgc cgaaagaggc
    13621 gatcccggac ccgcataatc tgacccttcg caccttcgtc aacggcgagt tacgccagca
    13681 aggcaccacc gccgatctga tcttcagcgt gcccttcctg atcgcctact taagcgaatt
    13741 tatgaccctg aatccgggcg acatgatcgc caccggcaca ccaaaaggct tatctgacgt
    13801 ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg ggccgcctgg tgaaccgaat
    13861 tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt ggatcaacgg caaaaatgtt
    13921 gcaggtaacg actacttcca gactaccaat ccggcaacgg gtgaagggct ggcggatgtg
    13981 gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg cagcgaaaga ggcgttcccg
    14041 aaatgggctg atctgccgat gaaagagcgt gcgcgcctga tgcgccgtct gggcgatctg
    14101 atcgaccaga acgtgcctga gatcgccgcg atggaaaccg cggacacggg cctgccgatc
    14161 catcagacca aaaatgtgtt gatcccacgc gcttctcaca actttgaatt tttcgcggaa
    14221 gtctgccagc agatgaacgg caagacctat ccggttgacg acaagatgct caactacacg
    14281 ctggtgcaac cggtaggcgt ttgtgcgctg gcgtcgccgt ggaacgtgcc gtttatgacc
    14341 gccacctgga aggtcgcgcc gtgtctggcg ctgggcaata ccgcggtgct gaagatgtcg
    14401 gaactctccc cgctgaccgc cgaccgcctg ggtgagctgg cgctggaagc tggtattccg
    14461 gcgggcgtgc taaacgtggt acagggctac ggcgcaaccg caggcgatgc gctggtccgt
    14521 catcatgacg tgcgtgccgt gtcgttcacc ggcggtaccg ccaccgggcg caacatcatg
    14581 aaaaacgccg ggctgaaaaa atactccatg gagctgggcg gtaaatcacc ggtgctgatt
    14641 tttgaagatg ccgatattga gcgtgcgctg gacgccgccc tgttcaccat cttctcaatc
    14701 aacggcgaac gctgcaccgc cggttcgcgc atctttattc agcagagcat ctatccagaa
    14761 ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg tgggcgatcc gaacgatccg
    14821 aatacccagg ttagggcgct tatcagtcag caacactggg aaaaagtctc cggctatatc
    14881 cgtctcggca ttgaagaagg cgcaaccctg ttggcgggcg gcccggataa accgtccgac
    14941 ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa cggtgctggc ggacgtagat
    15001 aaccgtatgc gcgttgccca ggaagagatt ttcgggccgg tcgcctgcct gctgccgttt
    15061 aaagacgaag ccgaaggctt acgcctggca aacgacgtgg agtacggcct cgcgtcgtac
    15121 atctggacac aggatgtcag caaagtgtta cgcctggcgc gtggcattga agcaggcatg
    15181 gtgttcgtca acacccagaa cgtgcgcgac ctgcgccagc catttggcgg cgtaaaagcc
    15241 tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag tgttcgcgga aatgaagaac
    15301 gtctgcattt ccatgggcga ccatccaatt ccgaaatggg gagtctgata tgggtaagtt
    15361 agcgttagcc gccaagatca cgcacgtacc gtcgatgtat ctctctgaac taccaggaaa
    15421 aaaccacggt tgtcgccagg gtgcgatcga cgggcataaa gagatcagca agcgttgccg
    15481 ggaaatgggc gtcgatacca ttatcgtttt cgatactcac tggctggtca acagtgctta
    15541 tcacatcaac tgtgcagacc attttgaagg cgtctacacc agcaacgagc tgccgcattt
    15601 tattcgcgac atgacctaca actacgaggg caacccggag ttggggcagc taattgccga
    15661 ggaagcctta aagctcggcg tgcgggcaaa agcgcacaat attcccagcc tgaaactgga
    15721 atacggcacg ctggtaccga tgcgctacat gaatgaagac aagcacttca aagtggtctc
    15781 catttcggct ttctgcacgg ttcacgattt tgccgacagc cgcaagctgg gcgaagcgat
    15841 tctgaaagcg atcgaacagt acgacggcac cgtggcggtc cttgccagcg gttcgttatc
    15901 gcaccgcttt attgacgatc agcgtgcgga agaagggatg aacagctaca cccgcgagtt
    15961 cgaccgccag atggacgagc gtgtggtgaa gctgtggcgc gaaggccagt tcaaagagtt
    16021 ttgcaatatg ctgccggagt acgccgacta ctgctacggc gaaggcaata tgcacgacac
    16081 ggtgatgctg ctggggatgc tcggctggga taaatacgac ggcaaggtgg agtttattac
    16141 cgagctgttc ccaagctctg gcaccggtca ggttaacgct gttttcccgc tgcccgcgta
    16201 aggacgtttt atgccgcact ttatcgtgga atgtactgaa aatattcgtg aagaagcccg
    16261 cttacctgag ctgtttgcca gtgttaacac tgcactggcg gcaacgggga tttttccgct
    16321 tgggggaata cgtagccggg cgcactggat cgatacctgg caaatggctg atggcaagca
    16381 cgattacgcc tttgtgcata tgacgctaaa aatcggcagc ggacgcagtc tggaaagccg
    16441 tcaggaagtg ggcgaaatgc tgtttgatct gattaaaacg cacttcgcct cactgatgga
    16501 gagccgttac ctagcgcttt catttgagat tgcagagctg cacccgacgt tgaatttcaa
    16561 acagaacaac gtacacgcgt tgtttaaata aatttgcacc agcttccagg ccggatgata
    16621 aggcgctgcc gcatccggca atgccacagg atatcgctat gttcgataaa cacacccaca
    16681 ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct
    16741 cgctggatta cccggagatc accatcgaag acgcctacgc cgtgcagcgc gaatgggttc
    16801 gcctgaaaat cgccgaaagt cgcacgctga aaggccacaa aatcggtctg acctcgaaag
    16861 cgatgcaggc aagctcgcag atcagcgaac cggattacgg tgcactgctg gacgacatgt
    16921 tcttccacga tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg
    16981 agctggcttt tgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgttt
    17041 acaacgccac ggactatgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca
    17101 tcgatccgga aacccagcgc ccgcgcaaag tattcgacac catttccgat aacgccgcca
    17161 acgccggagt gatcctcgga ggtcgtccga tcaaaccaga cgaactggat ctgcgttgga
    17221 tctccgccct gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggtgtgc
    17281 tgaatcatcc ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac
    17341 aactggaaac cgggcaaatc attctcggtg gttcgttcac cctcccggtt ccggcgcgta
    17401 agggcgacac cttccacgtc gattacggca acatgggctc cattagctgc cgctttgttt
    17461 aaggagagaa cgatggaaaa cagttttaaa gcggcgctga aagcaggccg tccgcagatt
    17521 ggattatggc tggggctgag cagcagctac agcgcggagt tactggccgg accaggattc
    17581 gactggttat tgatcgacgg tgagcacgcg ccgaataacg tgcaaaccgt gctcacccaa
    17641 ctacaggcga ttgcgcccta ccccagccag ccagtggtac gcccatcgtg gaacgatccg
    17701 gtgcaaatca aacaactgct ggacgtaggc acacaaacct tactggtgcc gatggtacaa
    17761 aacgccgacg aagcccgtaa agcggtacgc gccacccgtt atccccccgc cggtattcgc
    17821 ggtgtgggca gtgcgctggc tcgcgcctcg cgctggaatc gcattcctga ttacctgcaa
    17881 aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac
    17941 ttaccgcaga ttctggacgt ggaaggggtc gacggcgtgt ttatcggccc ggcggatctg
    18001 agcgccgata tgggttatgc cggtaatccg cagcacccgg aagtacaggc cgccattgag
    18061 caggcgatcg tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag
    18121 caactggcaa aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc
    18181 accctgctcg cccgcgccgc cgaagcgctg gcagcacggt ttggtaatag ttcttctatt
    18241 tcgatggcga agcaaaacaa caactctgtc tactaaacgt acgatgtgga gcgcatcatg
    18301 acgacctcta ccctgcaata taaagataat aaaactgttg aaattgaaca tcgtgtcatt
    18361 aataaattat ttcgtcgcct aatcattttt ctttttattc tgtttgtttt ttcgtttctt
    18421 gatcgcatca atataggatt tgccggactg acaatgggca aggatcttgg cctgacgtcc
    18481 acaatgtttg gcctcgccgc aacactcttt tatgtcacgt acgtcatgtg cgggatcccc
    18541 agtaatatga tgttagcgaa aattggcgct cgtcgatgga tcgctgggat catggtggtc
    18601 tggggaattg cttccacctg cacaatgttt gcaacaagcc ccgaaacact ctatctactg
    18661 cgtatgctgg ttggtattgc tgaggcgggt tttttacctg gtattttggt ttatctgacc
    18721 tggtggttcc cggcatatca tcgggccaga gccaatgcct tatttatgat agcgatgcca
    18781 gtcactatga tgctgggatt gatcctttca ggctacattc tggccttgga tggactgtgg
    18841 aatctaaaag gctggcaatg gctctttttg ctggaaggtc tgccatcggt cattcttggt
    18901 gtcgtcacct ggttttatct caacgacaca ccagaccagg cgacctggct tgatgaaaac
    18961 gaaaaacagg ctttaaaaac gatgattctc cgtgaacaag agagtgcatt tgcccatgca
    19021 tctgttccac gctcaacctt acgtgaagtc ctgaccccag ccatcatgct atatacactt
    19081 gcttattttt gcccgactaa tactctgagt gcgattaata tctggacgcc gcaaatccta
    19141 cagagtttca atactggcag tagcaacatc atgattggtt tgctggcggc catcccccaa
    19201 ttctgcacaa tttttggcat gatctggtgg agtcgtcgtt ccgaccgtct taaagaacgc
    19261 aaaaagcata cgattcttcc ctacctgttt gccgctgccg gatggttact cgcttctgcg
    19321 acagatcaca gcctcattca actgagcagc atcattatgg cctcaatggg atcatttacc
    19381 gcgatgacta tcttttggac aacaccggat caagttataa gcttgcaatc gcgtgccgtt
    19441 gcgatggctg tcatcaatgc tgtcggcaat gtaggttcag cagtcagtcc attacttatc
    19501 ggtattttga aagatgcaac cggcagtttt agctcaggtt tgtggtttgt agcaggattg
    19561 ctggtactgg gcgcgttagt catgacccga atccctatgg ggagtcggga acactctctt
    19621 tcatctgaac ttatcgcacc aaaaccgcaa taaggagtca tgatgtccag tgaagctatc
    19681 tttgaacatg gttatgaaaa tattgatttg gtccgggaat atgatgcccg ctacgctggt
    19741 gaagatgtgc attatgaaac atttgcccgg ttagcgaatt tctttggacg cgatatgcgc
    19801 cctcactggc acgatagata ttttcaactc cattatctgg tcactggacg cattacttta
    19861 caactggatg agcacttcta ttctgtgcgc gcaccgcttt ttgtactaac accaccatcg
    19921 gtgccacata ctttctttac tgatcctgat agcgatgggc atgttctgac cgtacgtcag
    19981 gagttaattt ggcccctggt agaaaagctc tggcctggta gtggagatgc cgcaatgcta
    20041 caaggtatct gtctgtcact ggagggaatg gaacatacac tggcggcatt aaatcattat
    20101 tggccgataa tatccctgga gtttcaacaa aataagcaag gacgggaaat tctattgcaa
    20161 tcactggcgc aaagtatttt tacaatccta ctgcgtgaag caccaccaaa tgatatttca
    20221 acatgcagcg tgcgcggcga gatgtgttta ttccagaagt ttaatcggct gattgacgaa
    20281 aattatcgcc aacatttaat ggtccctgaa tatgcaacaa tgctcggtat gagtgaatca
    20341 cggcttaccg agctgtgccg acgttttgcc aatcaatcgc cgaagagatt gattttcgaa
    20401 cgagtgagcc gggaagccag acggcttctg ctctatagcg agcaaagcat caaccagata
    20461 gcccttgatc ttggctacaa agatcccgcc tatttcgccc gttttttcaa ccgaatgatg
    20521 ggttgctcac caacacaatt ccgtggtcgc tgaccattta tcaacctgag agactcgaca
    20581 tgaaaatatc tttacgctca ctatttgtgg cgtgcctgat tgcgccagtg gcattcacct
    20641 acgcctccga accgcaataa atgaaaattg tactacttgg aacgaaaggt ggcccttctt
    20701 tattaaaagc tgaccgttta ccgatggcaa cggcacttat tattggcaat aaaacctggt
    20761 taattgatgc tggttacggg gccagcttac aactggtaaa aaataatatt ccactgcgcc
    20821 agattgataa tattttactc acccatttac acagtgatca tattctggac tatccatcag
    20881 tcttaatgaa tgcatgggct agcggtctgc aggacaaaac tattcatgta ttcggtccgc
    20941 caggcacccg agccatgacc gctgccagtt ggcaggtctt cgatcgggac atcacactgc
    21001 gtatggaaga agaaggaaaa cccgatcctc gcaaactcgt tacagctcaa gatgttggtg
    21061 aaggcgtagt ttataaagat gataccgtga ccgtgactgc attaaaggtc cctcattctc
    21121 ctttccctga cggtgaggct tttgcttatc gcttcgacac acaaggcaag cgaattgtct
    21181 tctcaggaga tacctcgtac tttcccccac tggcaacttt ctcagaaggc gctgatatac
    21241 ttgtgcatga ggccgtacat gagccctccg tcgctaagct ggcagacagt attggcaatg
    21301 gtaaaacact ggcaaaagcc attgcttctc accatacgac tattgaggat gtaggcaaaa
    21361 ttgccaatga agcaaaagtg aaaaaactca tccttagcca cctggtacca atcaccgttc
    21421 cggatgaaac atggaagcaa gaagcacaga aaactattca ggtcctatcg tcgttggtca
    21481 tgaaaatatg acgattacca ttccattcgc cccataaaaa attccgtccc cgttagccct
    21541 tgttagcggg gaatattatg atttgcctta ctgtaagttg atcacgatcg cagtttacta
    21601 tttaactcca ccttgctgtc gaaaagtacc aacgttcgcc tgaaacgtct cttcccgaca
    21661 accgcgttca acgcttcaat taaataacaa aaacaacaca caaaattaac aatcgtattc
    21721 cgattaatac tgtagaggtc gacatgaaac cagaagattt ccgcgccagt acccaacgtc
    21781 ctttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg tcgcgagatc tatatctatg
    21841 gcgagcgagt gaaagacgtc accactcatc cggcatttcg taatgcggca gcgtctgttg
    21901 cccaactgta cgacgcgctg cacaaaccgg agatgcagga ctctctgtgt tggaacaccg
    21961 acaccggcag cggcggctat acccataaat tcttccgcgt ggcgaaaagt gccgacgacc
    22021 tgcgccagca acgcgacgcc atcgctgaat ggtcacgcct gagctatggc tggatgggcc
    22081 gtaccccaga ctacaaagcc gctttcggtt gcgcactggg cgcgaatccg ggcttttacg
    22141 gtcagttcga gcagaacgcc cgtaactggt atacccgtat tcaggaaact ggcctctact
    22201 ttaaccacgc gattgttaac ccaccgatcg tcatttgccg accgataaag tgaaagacgt
    22261 ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt
    22321 tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat
    22381 gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtgaa
    22441 attaatctcc cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta
    22501 cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata acgtactgat
    22561 cccatgggaa aacgtgctga tctaccgcga ttttgatcgc tgccgtcgct ggacgatgga
    22621 aggcggtttc gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaactcga
    22681 cttcattacg gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg
    22741 tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga
    22801 ctcgatgtgt tcagaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc
    22861 actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattataga
    22921 acgcaacgtt accagtggcc tgatctacct cccttccagt gcccgtgacc tgaacaatcc
    22981 gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aacggtatgg atcacgtcca
    23041 gcgcatcaag atcctcaaac tgatgtggga cgccattggc agcgaatttg gtggtcgtca
    23101 cgagctgtat gaaatcaact actccggtag ccaggatgag attcgcctgc aatgtctgcg
    23161 ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc
    23221 ggaatacgac cagaacggct ggactgtgcc gcacctgcac aacaacgacg atatcaacat
    23281 gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacaacgc
    23341 ctgcgctttc atgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag
    23401 ggcgacgccg gacaatgcgg gattacggca acggctgtct gctcggtcac ggatacacca
    23461 ccgtcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac
    23521 ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcgcg ccacttcgcg
    23581 ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg
    23641 ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg aaggagagat ccgcgatgtg
    23701 caggcgattg gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca
    23761 gaaggtcacg gacttattta ctttaaacgc cgtttccatc cggtgatgct ggaaatggaa
    23821 gctgcgattt aagctccagc cccttcacgc attcgtcgaa ggggcatttt tattgtgatc
    23881 tcaatcacat atctacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc
    23941 gcttacatcc ctaaaataac cactcagtta tttaccttac tttacgcgca cgtatctctg
    24001 gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac
    24061 ggcttcggcc tatgattaac aaattactct atggattttg gtttgcagga aggcagcaag
    24121 tgagtgaatc cccgggagct tacaaaagta agtgtcagag gtgaacgaac gcagctgccg
    24181 cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac
    24241 tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag
    24301 ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg
    24361 tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg
    24421 accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca
    24481 accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgctggt ccgctggtgg
    24541 gaccggttct cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg
    24601 tagtgctggc cggtgcggta caggacttta tggtgctgtt tatctcctct cgccgtaacg
    24661 gcgcatctct cggtgagatg atcaaagaag agatggggcc agtaccgggg actatcgcgc
    24721 tgtttggctg tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta
    24781 aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc
    24841 tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtgggtgaa gtctctgtta
    24901 ttggtatcgt gctgctggtt gcctctatct acttcggtgg cgtgattgct cacgatccgt
    24961 actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg
    25021 cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa
    25081 ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tatcgtggtg ctgaacccgg
    25141 aactgaaaat gcctgcgatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg
    25201 ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc
    25261 tgatctcttc cggtacgacg ccgaaactgc tggctaacga aaccgacgcg cgtttcattg
    25321 gctacggcgc aatgctgatg gagtccttcg tggcgattat ggcgctggtt gcagcgtcca
    25381 tcatcgaacc gggtctttac ttcgcgatga acaccccgcc tgctggcctt ggcatcacca
    25441 tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag
    25501 acgttaccgc acacgcggca gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc
    25561 agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggtg
    25621 cgccgacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca
    25681 tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg
    25741 atgcgggtac ccgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt
    25801 tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc
    25861 tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt
    25921 ggccgctgtt cggtatctcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg
    25981 ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat
    26041 ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga
    26101 tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc
    26161 tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag
    26221 gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct
    26281 ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aacaccgtac gttccaatcc
    26341 cggaaggcgg cgtgaagatc tcttcgcacc actaaccgtg tttagccccg cttcggcggg
    26401 gctttgttct tatcagagtg aactatgttt ggtaacttag gacaggcaaa aaaatatctc
    26461 ggccaggcgg cgaagatgtt gattggtatt ccagactacg acaactacgt cgagcatatg
    26521 aagaccaacc atcccgacaa gccgtacatg agctatgaag aattcttccg cgaacgccag
    26581 aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct gttgttaatg gagagagcat
    26641 gaacccgatt gcagttaccc tactcaccgg ttttttaggc gcgggcaaaa ctaccctgct
    26701 gcgccatatt cttaacgaac aacacggcta caagattgcc gtgattgaaa acgaattcgg
    26761 cgaagtctcc gttgatgacc aactgattgg cgaccgcgcc acgcagatca aaacgctgac
    26821 caacggctgc atctgctgtt cgcgctccaa cgagctggag gacgcactac tcgacctgct
    26881 ggataatctc gacaagggca atattcagtt cgatcgtctg gtgattgaat gcaccggcat
    26941 ggccgatccc ggcccgatta ttcagacctt tttctcccat gagattttat gccagctcta
    27001 cctgctggac ggcgtgattg cgctggtgga tgcggtacat gccgatgagc agatgaacca
    27061 gttcaccatc gcccagtccc aggtgggcta cgccgaccgt attctgctga ctaaaaccga
    27121 cgtcgcaggc gaagcagaga aactgcgtga acgcctggcg cgcatcaacg cccgcgcacc
    27181 ggtctacacc gtcacccacg gcgacatcga cctgggcctg ctgttcaaca ccaacggttt
    27241 tatgctggaa gagaacgtcg tcagcaccaa accgcgtttc cactttatcg cggataaaca
    27301 aaacgatatt tcgtcaattg tggtggaact ggattacccg gtagatatca gcgaagtttc
    27361 ccgcgtgatg gaaaacctgc tgctggaatc ggcggataaa ctgctgcgtt acaaagggat
    27421 gctgtggatt gacggcgaac ctaaccgcct gctgttccag ggcgtccagc gcctctacag
    27481 cgccgactgg gacaggccgt ggggcgatga aaaaccgcat agcacaatgg tgtttatcgg
    27541 gattcagttg ccggaggaag agattcgggc ggcgtttgcg ggattgagga agtaagttcg
    27601 ggaaagatca actttttgag agttccaccg caacattatt acgagtggaa ctctttagat
    27661 atgtcagacc aaccaaagct tcttcaattg cgttttcatt ttttcatgtc tcattttcag
    27721 ttcagccaat ccccattctg aagaattaca aacatctttt gttaaataaa aagagctttt
    27781 actattaagt ttctcataaa tagatttctt tttgataaaa tcatagtgtt gtgtttcaga
    27841 atttttggaa ccacttatga gtgttaaatt acctatttta tgcagccagt agacatgctc
    27901 ttgaggttgg aatctattaa tccagtattc attaactaat gcttgaggaa gaatatgctc
    27961 aattgtgatt ctccccgtat aggttttaat cacgctctca tcttgttgct ccatatctaa
    28021 gcgaagcaaa attgctttta ttaagttcac ttgattcgga cgaggctcat ataaatcttc
    28081 atccagagca gctataaaac ctgaattatc tgcatgttgg tttatctgat ttatcacaga
    28141 atcaaaaggc atatcattat ttattgctac cagagcagaa taacaaacca tttctctttg
    28201 gcttttaatt tgttttttta accacccatg catatatacc ttctcaaagg cggtaataaa
    28261 ttgagaaaaa tcatctaaat taaaatcttc tgtacgagcc attcgattca tgaatgccat
    28321 aaccggagga atccactcat ctacccctaa attacttagc gatgctattt ttcgtcgtat
    28381 tgaagggtgt tcaaaatcat tctctaaaat cttagtata
//
LOCUS       DAZXUL010000049        28406 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZXUL010000049 DAZXUL010000000
VERSION     DAZXUL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28406
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.049"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..150)
                     /locus_tag="ACH3L1_002096"
     CDS             complement(<1..150)
                     /locus_tag="ACH3L1_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HFT4076823.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATF"
     gene            complement(256..1272)
                     /gene="phnD"
                     /locus_tag="ACH3L1_002097"
     CDS             complement(256..1272)
                     /gene="phnD"
                     /locus_tag="ACH3L1_002097"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFT4076824.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(1297..2085)
                     /gene="phnC"
                     /locus_tag="ACH3L1_002098"
     CDS             complement(1297..2085)
                     /gene="phnC"
                     /locus_tag="ACH3L1_002098"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFT4076825.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(2218..2661)
                     /gene="yjdN"
                     /locus_tag="ACH3L1_002099"
     CDS             complement(2218..2661)
                     /gene="yjdN"
                     /locus_tag="ACH3L1_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFT4076826.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(2819..3154)
                     /locus_tag="ACH3L1_002100"
     CDS             complement(2819..3154)
                     /locus_tag="ACH3L1_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFT4076827.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            3556..5784
                     /gene="crfC"
                     /locus_tag="ACH3L1_002101"
     CDS             3556..5784
                     /gene="crfC"
                     /locus_tag="ACH3L1_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFT4076828.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            5781..6659
                     /locus_tag="ACH3L1_002102"
     CDS             5781..6659
                     /locus_tag="ACH3L1_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFT4076829.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            6923..8425
                     /gene="proP"
                     /locus_tag="ACH3L1_002103"
     CDS             6923..8425
                     /gene="proP"
                     /locus_tag="ACH3L1_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFT4076830.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            8537..8626
                     /gene="pmrR"
                     /locus_tag="ACH3L1_002104"
     CDS             8537..8626
                     /gene="pmrR"
                     /locus_tag="ACH3L1_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFT4076831.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8602..9702)
                     /gene="pmrB"
                     /locus_tag="ACH3L1_002105"
     CDS             complement(8602..9702)
                     /gene="pmrB"
                     /locus_tag="ACH3L1_002105"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFT4076832.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(9703..10371)
                     /gene="basR"
                     /locus_tag="ACH3L1_002106"
     CDS             complement(9703..10371)
                     /gene="basR"
                     /locus_tag="ACH3L1_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFT4076833.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(10368..12011)
                     /gene="eptA"
                     /locus_tag="ACH3L1_002107"
     CDS             complement(10368..12011)
                     /gene="eptA"
                     /locus_tag="ACH3L1_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFT4076834.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(12115..13452)
                     /gene="adiC"
                     /locus_tag="ACH3L1_002108"
     CDS             complement(12115..13452)
                     /gene="adiC"
                     /locus_tag="ACH3L1_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFT4076835.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(13589..14350)
                     /gene="adiY"
                     /locus_tag="ACH3L1_002109"
     CDS             complement(13589..14350)
                     /gene="adiY"
                     /locus_tag="ACH3L1_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFT4076836.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(14675..16942)
                     /gene="adiA"
                     /locus_tag="ACH3L1_002110"
     CDS             complement(14675..16942)
                     /gene="adiA"
                     /locus_tag="ACH3L1_002110"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFT4076837.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATYSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(17141..18049)
                     /gene="melR"
                     /locus_tag="ACH3L1_002111"
     CDS             complement(17141..18049)
                     /gene="melR"
                     /locus_tag="ACH3L1_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFT4076838.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            18332..19687
                     /gene="melA"
                     /locus_tag="ACH3L1_002112"
     CDS             18332..19687
                     /gene="melA"
                     /locus_tag="ACH3L1_002112"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFT4076839.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            19790..21211
                     /gene="melB"
                     /locus_tag="ACH3L1_002113"
     CDS             19790..21211
                     /gene="melB"
                     /locus_tag="ACH3L1_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFT4076840.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(21350..21979)
                     /locus_tag="ACH3L1_002114"
     CDS             complement(21350..21979)
                     /locus_tag="ACH3L1_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFT4076841.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(22102..23748)
                     /gene="fumB"
                     /locus_tag="ACH3L1_002115"
     CDS             complement(22102..23748)
                     /gene="fumB"
                     /locus_tag="ACH3L1_002115"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFT4076842.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(23826..25166)
                     /gene="dcuB"
                     /locus_tag="ACH3L1_002116"
     CDS             complement(23826..25166)
                     /gene="dcuB"
                     /locus_tag="ACH3L1_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFT4076843.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(25737..26456)
                     /gene="dcuR"
                     /locus_tag="ACH3L1_002117"
     CDS             complement(25737..26456)
                     /gene="dcuR"
                     /locus_tag="ACH3L1_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFT4076844.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(26453..28084)
                     /locus_tag="ACH3L1_002118"
     CDS             complement(26453..28084)
                     /locus_tag="ACH3L1_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFT4076845.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            28265..>28406
                     /gene="yjdI"
                     /locus_tag="ACH3L1_002119"
     CDS             28265..>28406
                     /gene="yjdI"
                     /locus_tag="ACH3L1_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFT4076846.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
BASE COUNT         6756 a         6934 c         7419 g         7297 t
ORIGIN      
        1 gaaggttgcc atgttgccgc cgtctttaat cagcgtaagc ggggccattt ccgcgccctg
       61 ccacgagacg accagcacgt cgagaacaac ggcccagctc agaagcgaga accagctacg
      121 cttgggtggg gcgatggtga tggtttgcat gtttggctcc ggtctgtagg ccggataagg
      181 tgcttgcacc gcatccggca tcaacgccta cacattgtcg gatgcgacgc cggggcgtct
      241 tatccgacct acgatttact gcaccgcttt actcaccgaa ctcatcgcgc ttaacgcgtt
      301 gttcaggcgg tccaggtcat ccagctgcgc ctgaatctcg gtggttttcg ccagcttgtc
      361 ctgctcattc agccctttat tgcctttcac gctttgtatc tctttaaaca gtgcgagctg
      421 gcgaatcggc accagttgca ggtcgctgga agcgcggaac ggcgcccagc ccaggcgttc
      481 cagcaccgct ttctcttccg gcgtttttcc gtagttcata aagaagtcgt agatcttgtc
      541 tttggtagtt tcggacagat tcttgcgcca gacgatcgga tcgcctagga tcagcggcga
      601 tttccagatc accttcagct ctttaagctt ctccggcgcg gaggttttca gcttgtcgag
      661 gttttcggtg ttgttggtgg caacatccac ttgcttgttg gcgacggcca gcgcgttggt
      721 ttcatgcccg gcgttgacgg tgcgcttgaa gtcgctggcg gagatattgt ttttagcgaa
      781 gacgtagtaa ccggggacga ggaagccaga ggtggagtta ggatcgccat tgccgaaggt
      841 gagatctttc cgcttcgcca gcagatcgtt caggttgttg atcgggctgt ctttgttgac
      901 gatcaacacg ctccagtaac ccggcgatcc atccgccgcg accgtctggg cgaagacctg
      961 gccattggcg cgatccaccg cttccatcgc cgacaggttg ccgtaccagg caatatccac
     1021 tttattgaag cgcatcccct ggataatgcc cgcgtagtcc ggggcaaaga aggcgttgac
     1081 cttcacgccc agtttcttct ccatatcctg taaaaacggc gtccattgcg gtttcaggtt
     1141 ttgctgtgat tccgttgaaa taatgccgaa attcagcgcc ttttcctgct cttcggcgtg
     1201 tgccggggtt aacagggtgc tgaggctgaa catgctggtg aaggccagcg aggcaattat
     1261 cttagcgttc attcgttttc ctcaatgatg gggatatcag gcagctttcg cgttctcttc
     1321 gatgcggtta atgctgcggt agagatggtc aaaacgttcg ttatcaaaca gttggctgct
     1381 gccgtcgtag aagacgtgcc cctgacgcag ggcgacgatg cgttcgcagt agcgcagggc
     1441 gtaatccacc tgatgcagcg tgacgaccac ggtgatgccg tcgttctggt tgatgtcgcg
     1501 cagggtgtcc atcacgatgc gcgctgattc cgggtccagc gaggcgatgg gttcatcggc
     1561 cagaatcact ttcgcctgct gcatcagcgc acgggcaatc gccacacgtt gctgctggcc
     1621 gccggagagg gtggaaacgc gctgatgggc aaaatgcacc atgccaacgc gggtcagcgc
     1681 ctgtaacgcg cgctgtttct gctcgccggt gaaccagcta aaacaggtgc gccagaacgg
     1741 cgtgctgccg agcgcgccaa tcagcacgtt ctccagtacg ctcaggcggt tcaccaggtt
     1801 gaattgttga aagatgtagc cggtgttggc gcggcttttg cggatatcgc gggccaggcg
     1861 gccttcgcgc tggacagtgc ggcccagtag ctcgatatgg ctaccggcgg atttatcgcc
     1921 ggtaatcaaa ccgcttaaat gacgtaaaag ggtggatttg ccggaacccg acggcccaag
     1981 cagagccacc atttcactgt gatgaatgtt cagatcaacc gcatgcagcg cttgatgctg
     2041 attgaaggtt ttggcgagct tctcgacacg gataatcgtt tgcatgatgc agcctcccta
     2101 aaaaagtggc tccatcgtgg cggattattg tgacgatttg gttaattaaa agtgactaac
     2161 agatgaagag ttaacgggaa ttcgatgaca gtgcgggagg gcgggcctcc cggcgggtta
     2221 ttgcgttggt tgttgtttga cgacattaat catccacggt acgccaaatt tatcggtgac
     2281 tttgccaaag ccatgcgccc agaaagtttc ctgccaggcc atttcgattt ttccgttagc
     2341 ggcaaggtta tcaaaccagc gttttccttc ttcgacctgt tgcgaatcga gcaccagcgt
     2401 aaagccggag tagctggctt ttcctgacgg tatagcatcg ctcatcatga tgtcgcttcc
     2461 ggcaatgcgc acgttggcat gagcgatggc ggtatcggga aattgcattc cggaagggca
     2521 gttctcggcg ctgtcctgcg ctgatttcgg catttcgccg aagctgattt tatagagcag
     2581 ttccgcgccc aacgtacgtt gataataggc aatcgcgtcg gaacagttac cggcaaaaga
     2641 gaggtaggga cttaacggca tgatggtgac ctcagttaag agaagccagt taagtgtagt
     2701 tcgaaattta tacagatgag aggctgcctg ataagacgcg caagcgtcgc atcaggcagt
     2761 cggcatcgtt gccggatgcg gcgcgagcgc cttatccggc ttaccgatca caatacaatc
     2821 agttcttttt cacaaactca gatttcagtt tcatcggacc aaaaccgtcg attttgcaat
     2881 cgatgttatg gtcgccttca accaggcgga tgtttttcac tttggtgcca attttcagca
     2941 tcgaagagct acctttcacc ttcagatctt taatgatggt aacgctgtcg ccgtcagcca
     3001 gcagattgcc gttagcatct ttaacgatca gctcgtcgct ttcctgtgca ggttctgcgt
     3061 cgttccattc gtaggcacat tccgggcaga tgtacatgcc gttatcttcg taagtgtatt
     3121 cggagttgca ttttgggcag tgtggtaatg acatgtggat ttcctcaaaa gtcagcaggg
     3181 caaaagcgcc aaaaaacggc agattgccga aaaaggccgc aattatacac aaaatccctg
     3241 agtttgtcgg gggtactgct gattttagac gacgatcgtt ttcatttgaa aggaaacgca
     3301 aaaagaaaat aatctttttg cctctattta ttatttaaga taatgttaat gctctacttg
     3361 ttcttttttt attcacatga tatttttatc tttctgacgt tttaaacgca tcagaagacg
     3421 ttatttattc tgataaaaaa acaacacact aaatataaaa ttatttcttg cagtaattat
     3481 aaaagccgaa aacagaactc aaaaacaatc tggctaaata aaagtaacaa aatttgcttt
     3541 aaggaagaat tttctatgta cacacagacc ctgtatgagt taagtcagga ggctgaacgc
     3601 ctgttacagc tttctcgcca acagttgcag ttactggaaa aaatgcctct ctctgtaccc
     3661 ggagacgacg cgccacaact ggctttaccc tggagtcagc ctaatatcgc cgaacgtcac
     3721 gcgatgctga ataatgagtt gtgtaaaatt tcccgactgg aaatggtgct ggcaattgtc
     3781 ggtaccatga aagcagggaa atcaaccacc attaatgcca ttgttggtac ggaggttctg
     3841 cctaatcgta atcgcccaat gactgcgctg ccgacgctta ttcgccatac gcccgggcaa
     3901 aaggaaccgg tactgcattt ttcacatgtc gcgccaatcg attgtttaat tcaacaatta
     3961 caacagcgcc tgcgtgattg cgatattaag catctgaccg atgtgctgga aatagataaa
     4021 gatatgcgtg cgcttatgca gcggatcgaa aatggcgtcg ctttcgaaaa atattatctg
     4081 ggtgcccagc ctatttttca ttgtctgaaa agtttgaatg atttagtgcg actggcgaag
     4141 gcgctggacg tcgattttcc tttttctgct tacgccgcca ttgagcatat tcccgtgatt
     4201 gaagtggagt ttgtccatct ggcggggctg gaaagttatc ccggtcagtt gacgttactg
     4261 gatacccccg ggccaaatga agccgggcaa ccgcatctgc aaaaaatgct taaccagcag
     4321 ctggcacgcg cctcggcggt actggcggtg ctggattata cgcaactgaa atcgatctcc
     4381 gatgaagagg tccgtgaggc gattttggcg gtggggcaat cggtgccgct gtatgtgctg
     4441 gtcaataagt tcgatcaaca ggatcgtaac agtgacgacg ccgaccaggt gcgggcactg
     4501 atttccggga cgctgatgaa aggctgtatt acgccacagc agatattccc ggtgtcgtcg
     4561 atgtggggct acctggcgaa tcgggcgcgc tatgagttag ccaacaacgg taagttacca
     4621 gcgccagagc aacaacgctg ggtggaagat tttgcccatg ccgcgctcgg caggcgctgg
     4681 cgtcatgccg atctggcgga cctcgaacat attcgtcatg ctgccgatca gttgtgggaa
     4741 gattcgctgt tcgcccagcc aattcaggcg ttgcttcatg ccgcttacgc taacgcctca
     4801 ttgtatgctc tgcgatctgc tgcgcataaa ctgttgaatt acgcgcagca ggcgcgggaa
     4861 tacctggatt ttcgtgcgca cgggttaaac gtcgcttgtg aacaattgcg gcaaaatatc
     4921 catcaggtcg aagaaagttt gcagctattg caactcaatc aggcgcaggt gagcggcgag
     4981 attaaacatg aaatcgagct ggccctgacc tccgccaacc actttctgcg tcaacagcaa
     5041 gatgcggtga atgcgcagtt agccgccttg tttcaggatg attcggagcc gttaagcgag
     5101 attcgtaccc gctgtgagac actgttacag acggcgcaga acaccatcag tcgcgacttt
     5161 acgctgcgtt ttgccgagat ggaatccacc ctttgccggg tgttaaccga tgttattcgc
     5221 cccattgagc aacaagtcaa aatggaattg agcgagtcag ggtttcgtcc tgggtttcat
     5281 tttcctgttt ttcacggcgt agttccccac ttcaacactc gccagctgtt cagtgaagtc
     5341 atttcgcgcc aggaagcaac ggacgagcag agcacgcgtt taggcgttgt gcgtgagact
     5401 ttttcgcgct ggttgaatca gcccgactgg ggacggggaa atgagaaatc cccgacagaa
     5461 acggttgatt acagtgtgtt gcaacgagca ttaagcgcag aagtcgatct ctattgccaa
     5521 caaatggcta aagttctggc agagcaggtc gatgaatctg ttacggcagg catgaatact
     5581 tttttcgctg agttcgcttc atgtttgaca gaattacaga cgcgtttacg cgaaagtctg
     5641 gctctgcgtc aacaaaatga atcggtggtc aggctgatgc agcagcaatt gcagcagact
     5701 gtgatgactc acagctggat ttacaccgac gctcagctgt tacgcgatga tattcaaaca
     5761 cttttcacgg cagaacgata ttgaccaaga cgttactgga cggccccggt cgcgtgctgg
     5821 agtcggttca tccgcgcttt ttagtggatc tggcgcaggg cgatgatgcc cgccatcctc
     5881 aagcccatca gcagcagttt cgtgaacgac tgatgcagga acttctttcg cgtgtgcagc
     5941 ttcagacatg gacgaacggc ggcatgttaa atgcgccgct tagcctgcga ctgacaatgg
     6001 tggaaaaact ggcgtcgatg ctggatcccg gtcatctggc gctgacgcag atcgcgcagc
     6061 atctggcgct gctgcaaaaa atggatcacc gccagcactc tgctttcccg gagctccccc
     6121 agcaaattgc cgccttgtat gagtggtttt cagcccgttg tcgctggaag gaaaaggcgt
     6181 taacgcaacg aggcctactg gtgcaggcag gtgatcagag cgagcaaatt tttacccgct
     6241 ggcgtgctgg ggcgtataac gcctggtcgt tgcctgggcg ctgttttatc gttctggagg
     6301 agttgcgctg gggtgcattt ggcgatgcct gccgtctggg aagcccgcaa gcggtggtgt
     6361 tgttgctggg tgatttgcgc gagaaagcga cacaacatct ggcagagagt atcaatgcgg
     6421 caccgactac gcgtcactat taccatcagt ggtttgcctc ttcgaccgtt ccgacgggcg
     6481 gggatcatgc tgatttttta agttggctgg gaaagtggac cacggcagat aaacaacccg
     6541 tttgctggtc agtgacccaa cgctggcaaa ctgtcgcgct ggggatgcca cgactctgtt
     6601 cagcgcagcg tttggtgggg gcaatggtcg aggaaatctt ctctgtaaat ttggcgtaaa
     6661 taatcagtta catcaatgag tcctaaacga aatccatgtg tgaagttgat cacaaattta
     6721 aacactggta gggtaaaaag gtcattaact gcccaattca ggcgtcaact ggtttgattg
     6781 tacattcctt aaccggaggg tgtaagcaaa cccgctacgc ttgttacaga gattgcatcc
     6841 tgcaattccc gctccccttt tgcggccgtc gcgctgattt ttctggcgtt tgcggaaatg
     6901 ggccaactct gcgaggaaag ctatgctgaa aaggaaaaaa gtaaaaccga ttacccttcg
     6961 tgatgtcacc attattgatg acggtaaact gcgtaaagcc attaccgcag catcactggg
     7021 taatgcaatg gaatggtttg attttggtgt ttatggtttt gttgcttacg cattaggtaa
     7081 agtttttttc ccgggggctg accccagcgt gcagatggtt gctgcacttg ccactttctc
     7141 cgttcccttt ctgattcgac cgcttggcgg actcttcttt ggtatgttgg gcgataaata
     7201 tggtcgccag aagatcctcg ctatcactat tgtgattatg tcgatcagta cgttctgtat
     7261 tggcttaata ccgtcctacg acacgattgg tatttgggca ccgattctgc tgttgatctg
     7321 taagatggca caaggtttct cggtcggtgg tgaatatacc ggggcgtcga tatttgttgc
     7381 ggaatactcc cctgaccgta aacgtggctt tatgggcagc tggctggact tcggttctat
     7441 tgccgggttt gtgctgggtg cgggcgtggt ggtgttaatt tcgaccattg tcggcgaagc
     7501 gaacttcctc gactggggct ggcgtattcc gttctttatt gctctgccgt tagggattat
     7561 cgggctttac ctgcgccatg cgctggaaga aactccggcg ttccagcagc atgttgataa
     7621 actggaacag ggcgaccgcg aaggtttgca ggatggcccg aaagtctcgt ttaaagagat
     7681 tgccactaaa tactggcgca gcctgttgac atgtattggt ctggtaattg ccaccaacgt
     7741 gacttactac atgttgctga cctatatgcc gagttatttg tcgcataacc tgcattactc
     7801 cgaagaccac ggggtgctga ttattatcgc cattatgatc ggtatgctgt ttgtccagcc
     7861 ggtgatgggc ttgctgagtg accgttttgg ccgtcgtccg tttgtgctac ttggtagtgt
     7921 tgcactgttt gtgttggcga tcccggcgtt tattctgatt aacagtaacg tcatcggcct
     7981 gatttttgcc gggttactga tgctggcggt gatccttaac tgctttatgg gcgttatggc
     8041 ttctaccttg ccagcgatgt tcccgacgca tatccgttac agcgcgctgg cggcggcatt
     8101 taatatttcg gtgctggttg ccggtctgac gccaacactg gcggcctggc tggtcgaaag
     8161 ctcgcagaat ctgatgatgc ctgcctatta cctgatggta gtggcggtga ttggtttaat
     8221 caccggcgta accatgaaag agacggcaaa tcgtccgttg aaaggtgcga caccggcggc
     8281 gtcagatata caggaagcga aggaaattct cgtcgagcat tacgataata tcgagcagaa
     8341 aatcgataat attgaccacg agattgccga tttgcaggcg aaacgtaccc gcctggtgca
     8401 gcaacatccg cgaattgatg aataagctga aacggatggc ctgatgtgac gctgtcttat
     8461 caggccaatt gaactcttaa ggttcactta atctctgacg cgcatactct cctccaggtt
     8521 aacggaggag agtgcaatga aaaaccgtgt ttatgaaagt ttaactaccg tgttcagcgt
     8581 gctggtggtc agcagctttc tttatatctg gtttgccacg ttctgatctt tcttcagccg
     8641 tacccaggcc cgcgtgccgg aagtctcttg ccggttttgc aggaaaaact gcccgtgatg
     8701 caactgtgta atgcggctga caatacttaa ccccagacca atcccgccat aacggctgtc
     8761 catacgtaca aacgctttac tcaactcccc gcatttactc tcatcaatac ctggtccttc
     8821 atcttcaact gccatgaccg ctccgccatc ttcttgcagc ttaatcataa tgttgctgcc
     8881 ttgcgggctg taacgatggg cgttttctac caggtttcgc aataacatcc gcagcagggt
     8941 tgcatcaccc tgaacggtga tgtcggcggc gctctctggc aatagcaggg tttgctgtcg
     9001 ctggtcgagc atggtactga gttcgtcata cgaggggaga atgacatctt ccagcagttt
     9061 tacatgttga taattaccgg aagaaaatga ctgtccggca cgcgccagtt gcagcagctg
     9121 ggagacgctc tccatcatct gatcaagccg tgccactaac ggtgctacat caatgtgatg
     9181 cgttttcgcc agcagttcca gatgcaaacg cacccccgcc agtggcgttc gcagttcgtg
     9241 cgcgacgtca gcggtaaaca acctttcgtt atccagcgtg ctggtcaggc gactgaccag
     9301 atcgttcaac gccgaaacca ccgcttcgat ttcgagggtg gcgctgtgaa tggcaatggg
     9361 cgttaagttg tcggcggtgc gcgcttccag ctctttttgc agctccgcca gcgggcgggt
     9421 gatgcggcgt accgcctgat agcagataaa tagcgtcagg ctgaccataa agacgccggg
     9481 gacaatcagg ctggcgaccg cctcgcggat ctcacgcatg atgtggcgat cgttgttgcg
     9541 attgtcgcgt agcgcctgct caaacagctg aatctgctcg gtactttcat gccatagcca
     9601 gaagacgctg atcagctcaa acaccaacaa aatggccccg atggtcaata tcagccgttg
     9661 gcgcagcgat ataggtcggc gcagaaaacg catcagattc aattagtttt cctcattcgc
     9721 gaccagcata tagccaaagc cgcgcacggt gcggatacgg gctttgccca ctttgtcgcg
     9781 cagattgtgg atatgcactt ccagggtgtt ggtcgagggt tcattgtccc agttatagat
     9841 gtcgttgtag agaatttccc gatgtaccgg actgcctgct ttgagcatta accgtgacag
     9901 cagagcatat tctttgggcg tcagaatcaa ctcttcaccg cccatccata cctgacggcg
     9961 acccatattc agcgtcagat tgccaacaat cagctcactt tcgccctgat tattatggcg
    10021 tcgtagcagg gcgcggatac gggcgtgtaa ctcttccagc gcaaaaggct tcaccagata
    10081 gtcatcggca ccgacatcca gcccggcgat tttgtcggtc agcgtatcgc gagcggtgag
    10141 gatcagtacc ggcagggtgt attttttctg ccggatacgg gcgagaaaat gcagtccatc
    10201 ttcgtcgggt aaccctaaat ccagtaccac caggctgtaa tgaccgtcct caaggctttg
    10261 ttccgccatc cgcgcggttg tcacgccatc gcacgcgtag ccttcggttt gcgccgccag
    10321 aatcagtccc tgcaataaca gcgtatcgtc ttcaacaatc agaattttca tttactcact
    10381 ctcctgcaag tttgcagaat atcatccgcg gcctggtaat acttcgtctc aacgccagtt
    10441 aatcccaata gcgtggagaa taaattgtct tgtgaatagt gttgcgtttg cgcctgtttt
    10501 tgcaggcagt tctggtcaac ctgataccgc ttttgataat cctccgacag ccacagcagc
    10561 attggcacct gtttttggct atccggggcg atggcataag gcagaccgtg cagatagatg
    10621 ccattttcac ctaacgattc accgtggtca gaaagataaa ccaggctggt ggtaaattta
    10681 tcctgatgtt ctttaagcag attaatcgct ttatcaacaa tatagtcgac gtaaaccagc
    10741 gtgttgtcgt aagtgttcac cagttgctct ttggtacagg tctggatctc attggtgtcg
    10801 caggttgggg taaatttcct gaactgcggc ggatagcggt tgtaataggt cggaccgtgg
    10861 ctgccgatgg tgtgtaagac aatcacgcca tcaccctgca ggttattgat gtactcttcc
    10921 agcccgtgga acagcacttc gtcatagcat tcgccgttga tgcactgatc aggcagattc
    10981 agcgcggtga cgttctggtg aggcacgcgg tcgcaggcac ctttacagcc gccatcgttg
    11041 tcattccaca gcacgttgat gcccgctcgc tgaatgatat ccagcacgcc ttcctggtgc
    11101 tgtgccagct cttctttgta gtgctcacgc ggcatatccg agaacatgca cggtactgaa
    11161 actgccgttg ccgtgccgca agatgcggta ttagggaaat agaccacgtt atctttcgcc
    11221 agccgcgggt tagtttcacg cgggtagccg ttgagggaga agttctccgc ccgcgaggtt
    11281 tcgccgacaa tcaggatggt caaattttta cgtttttcgt tctgcattaa cgggttgcgg
    11341 tgcgcgtctt caccaattcg caccagcggc agatttgcca gtcgctgatg ggagtaccat
    11401 gaccagctgg caacaatgct gttagagggg cttaaggatt tcaccagctc tttgttattg
    11461 cggaacaacg aggcgtagtc tttataaaac agtgcggcga ccagcaaaat cagcagtaca
    11521 gaaaccagaa tattggctcc acggaaaaga acactgcgca gacgcgaggt ggcaggtttg
    11581 atttttagcc agcaggcaat cagctcagca agcacgccgc tgaatcccag cgttaataac
    11641 atttgcggtg tcatcagcgc ataactttct gctggagtgg tatcaataat attggcaatc
    11701 atcgagcggt cgatgacgat gccgtaagtc attatgaaat attgtgcagc cgcgccaatc
    11761 agaataaaca ggcaggccag tggtcgatta agccataaga aagagcttag tgtcaggaca
    11821 atattaatca cgctgaaagc gacaaccggc atcgacaaga aaaccagtac gttatgcagc
    11881 gaatccagcg gcagcgcctg caacacctgt ttaaaaaagg caatattcag gcagatagag
    11941 atataaaaag cggccaacaa tagccaggcg agtaaattca aagagggtct ttttagtagg
    12001 cgcttcaaca tcacggtgtt tccatcgaac aaagtgcgca tatgctcgca aagcaaaatt
    12061 aagccaacct taagttctta aggttggctt ttatgtttgc tggattaagg ggaattaatc
    12121 tttgcttatt ggtgcatcta agggatacgg gtttttatgt agccggttgt aattcagcgc
    12181 atacatggcg gtgatgacca tcagggtgac aaatgaccac ataacctctt tcgccccgga
    12241 ccccaccacg gcccaaatgc agtagaggaa ggcaatggta gtaacagcca gatatgccgg
    12301 gcgtgcttta ccaaagtgac cgtggccgag cagcagtaac gccgcacagg tgtaaagata
    12361 tggcaccagt gtaaagatga ccgagacgga agaaaccaga ccgaactctt tggtcgcgtt
    12421 tggtgaaatg ctgctgagct ggaagatggt catcaaaata ccgacgataa tcagccccgc
    12481 cactggcgta cccgctttat ttacacgggc aaaaatcggt gggaacagtc cgtcatcggc
    12541 agcggctttc gccgtttgac ccgccagcaa cgtccagccg cccagtgaac ctaagcaacc
    12601 cgcagctgcg cagaaggaaa caatggcccc agcggtgtca cccaacgcca tccgtgcggc
    12661 atcaccgaat ggcgaggcag aaacgcgcag tgcggcatta gggatcatcc ccataatcgc
    12721 ggtggtggag agcacatagc aaacggcggc aatcaatacc ccaccaatgg tggcgatagg
    12781 gacattgcgt ttcgggtttt tcaccacacc tgcggcaacg gaggcacttt ccacaccgat
    12841 gaacgaccac agcgtaacgt taagggtact ttgaatcgca ccgaaggtgc ccaggccgct
    12901 gacgttccat gccgccatat aggtttcacc acggaaccag aaccagccaa atacggcaat
    12961 cccgacgatg gggatcagcg ccagcacagt ggcaacggcc tgcacgcggg tgatcatttt
    13021 cggaccgaca atgttcagca ggacgaagat ccacagcacc acgacgcagg tgatggttaa
    13081 taccaatgga tctttcagaa tcgggaagaa gtaacttaaa tatcctacgc caatgaccac
    13141 catggcgata ttgccgatcc agcaggccag ccagtagagg acgttggttt gataaccgag
    13201 aaacgggcca aagcagcggc gggcgtaagc gtaagaacca ccaggacttg ggtcgaggaa
    13261 cgacattttg gcgtatacca tcgagagccc cagcgcaccg ataatcgtca ccaaccatcc
    13321 ataaatggca atcccgccag tggaggccag gtttgcaggt aacagaaaaa cacctgaccc
    13381 cataatattc cccgacacca tcagggtgac ggggattaag cccactttgt gagcatcagc
    13441 atccgaagac ataattaaac tcctgcgaag gcgagcttcg tgacaataaa ttacgtcata
    13501 tcatacgcct gcattcgata gttgaattat tatcgaccgg gttatcgctg atgccgttat
    13561 taatatagct cttcgttaag gttaaaaatc aggcgacagt gcgttctctg tgctgactaa
    13621 cataatgcag cggcgtcata ccgtagaagt ctttaaagac agaaataaag tacgacgtac
    13681 tgttgtagcc gcaggactgt gatacctgag agatattttt accgtccatc attaattcat
    13741 ttacggcata acgcatgcgg caggtggtga ttatttggct ataactggtg ttttcgcttt
    13801 tcaacttttt ctttaacaga cttgggctaa gacataaaca actggctacc atactaagat
    13861 tccagtcttt gtgaatatcg ctttcaataa tttgataaac gctgtcactt acacaattac
    13921 gtaacatata catcattaat gaaaggaatt ttttactgtc gagaaaacgg gatagcaccg
    13981 tgaataataa tgcgcgtgtt cgttcctttt ctgactcagt ttctgcgggc atcatgctgt
    14041 gttgcgcggc aacgcgaaag acgtctggcg tcaggcatgg cagggtgagt atgggcgtag
    14101 cgctacgttg ccatactggt atttgcgaga gatctttatt cagaaaccgg aggtaatctt
    14161 tgatgatgtc gtgactaata tgagcaacca aagtgttgtt aagagaggag acgtcgataa
    14221 tattattttc acaatttaat aacgccatat ggttagcttt aaggctaata ggttctttcc
    14281 cattcaccct tatccagaca tctttttcag tcaataaaac aatacaaggt tggtcgctgc
    14341 aaatcctcat acatgtactc ctgagtgcga ataaaagacc agttctcgct aaagcaaagc
    14401 gataccggga aatggttata taattgcatg atgaatataa acatgcataa aaataacgag
    14461 ttttactaat attacaaatt tttatagtaa tttctcatgt acatattttt ttacaggata
    14521 aataacaggc aaaaaaaagc gtggggtggg gccgcgcaaa aagtatgaca tttttgtttg
    14581 cagcaatttt tatgaaaacg ttgccatact caaaaggcct aaggccaggc ggcgtaatgt
    14641 tatttaaaca attacgcctt cagcggaata gtggttacgc tttcacgcac ataacgtggt
    14701 aaataccgtc aataatttca gtcccttcag tttcgtgttc aaatctaggg aaatggtggt
    14761 cccaggattg cagcgagcgt aaataactta cttgcggact gtttttatcg ccgaagtttt
    14821 caccagacag cagcatcggg attcctggcg gatacgggat aactgagttt gccgcgatgc
    14881 gtcctggcag attttcaatg gataccagtt cgacattgtt gtcgacaatc gcgttgtacg
    14941 cttcacgcgg ggtgatttcc gccaccggca ggccggaata ggcttcgttc aaccgtgcgc
    15001 cagggttgtt ttctttcagc caggcaaaca tggtgtcacc cagatcgtga atccccatgt
    15061 tcgcgtaagt gtcaggatat tgttcaacaa gttccggcat cacctgcgcc agcggtgtgt
    15121 tggcgtcata gtggtgtttg aaggagcaaa gggtgttaac cagagtcccc catttcccac
    15181 gggttacgcc catagagaac aggaacataa tttggaagtc agtggtgcgg gtaggcacaa
    15241 tgccgtggcg accaagccag gcagtgacca gcgccgccgg aacaccggtt tcttccagtt
    15301 caccatcttc acccattccc ggagcaagga tgctgacttt aattgggtcg agcatactcc
    15361 agttatccgg aatatctttg aagccgtgcc agctttcgcc cggatgcatt acccagcagt
    15421 cctgaacggt ggtcagcagt ttggttggtg cgtcagcaaa gtcataggtt ttgccggttt
    15481 gtgggtcggt gacgacttct ttgttccacg gtttgaagaa ccagctaccg tcagcggtga
    15541 actctttata tagccgcgcc atcgcctgac ggaaatcaac cgcttcgtca atcacttcct
    15601 gtgtcagtga caggccgctg ttgccgtcca tcatcgacac cgccacgtcg ttggatgcgc
    15661 agatggcata cagcggggag gtggtggcat gcatcatgta ggcctggttg aagcgggaga
    15721 agttaatcgc cccacgacct tcacgtacat gaatataaga agcctgtgac agcgcattca
    15781 gcagtttgtg gatggagtag gtggcgaaaa cggtaggacc gttgtgatcg ccaggttcgc
    15841 cgcgcatggc atagtgatcg gcatagatcg ggttgaaacg tgcatagccg taccaggctt
    15901 cgtcaaagtg cagacgatcg gaggtttttt ccagcagatc ctgcgcttct ttagcgttat
    15961 aacacacgcc gtcataggtg cagttggtta ccacgcagta agacggtttt tgcccggctt
    16021 tgtctttggt cagcgggctt tcactgattt tcttttgcaa ggtttcaggt tgcatttcct
    16081 gcggatagat tggcccgata atgccgtagc ggttgcggct tggcaccata tagaccggtt
    16141 tcgcgcctgt cagcatcaaa ccttgttcga tggatttatg gcagttacgg tcaacgacca
    16201 cgacatcgtt atcggtcatg caagcctgca tgatggtgcg gttagagccg gaagtaccga
    16261 cgactaccga ccaggagcga tcggcaccaa atacgcgtgc ggcatatttt tcgctttcgc
    16321 caaatgcgcc agtatggtca agcaaagaac cgagggaagt tcgttcgatg cccatgtcgg
    16381 tacggaacag attttcacca tagtagtcat ggtagaaacg tccggcgggt gttttggtaa
    16441 aaccaacgcc gccctggtgg cctggcgctg cccaggaata ttcatggatg tcactatatt
    16501 tcatcagcgc gctgaacagt ggcggcaaca gctgctggcg gtagcgggtc atcgcggcaa
    16561 cggcgcgtcc ggcgataaag tcggcggtat cttccagaat ccaggcgaat tcatcgacaa
    16621 gctccagcag atcccggtcc attgcggcga gggctttttc ccgatcgccc aacaggaaga
    16681 ccggcacgtt ttgttggcgc tcatgaagct taccgatcaa ttgtctgacg ttttgatgtt
    16741 cgtccggatg ctccatttga tagctgaaca tcaggcagtc aatggcttcg tttgaagaga
    16801 gaatggcaaa accatcatca aaggaggtgg atttaatcac ggtaacattt tgctggctta
    16861 aagcatctgc cagacgctca acggcgttac cgacccaggt gtcttgatgg agaaactcgc
    16921 tttcaacaat taatactttc atcattgctt acccggttat gaaggaatct tcgttgcggg
    16981 caagtatctt ccggcctttt cgggctgtaa agcgcgtgaa aaacaaacat ttttgaaata
    17041 tttagtttta taaacaatac gttatgtgat tattttaaat tattttcact gcttattatc
    17101 gacggctaaa ctattttttt ggctgatact gatatcgtct ttagccggga aacgtctggc
    17161 ggcgctgttg gctaagtttg cggtattgtt gcggcgacat gccgacatat ttgccgaacg
    17221 tgctgtaaaa acgactactt gaacgaaagc ctgccgtcag ggcaatatcg agaatacttt
    17281 tatcggtatc gctcagtaac gcgcgaacgt ggttgatgcg catcgcggta atgtactgtt
    17341 tcatcgtcaa ttgcatgacc cgctggaata tccccattgc atagttggcg ttaagtttga
    17401 cgtgctcagc cacatcgttg atggtcagcg cctgatcata gttttcggca ataaagccca
    17461 gcatctggct aacataaaat tgcgcatggc gcgagacgct gtttttgtgt gtgcgcgagg
    17521 ttttattgac cagaatcggt tcccagctag agaggctaaa tcgcttgagc atcaggccaa
    17581 tttcatcaat ggcgagctgg cgaatttgct cattcggact gtttaattcc tgctgccagc
    17641 ggcgtacttc aaacgggcta agttgctgtg tggcaagtga tttgatcacc atgccgtgag
    17701 tgacgtggtt aatcaggtct ttatccagcg gccaggagag aaacagatgc atcggcagat
    17761 taaaaatcgc catgctctga caggttccgg tatctgttag ttggtgcggt gtacaggccc
    17821 agaacagcgt gatatgaccc tgattgatat tcactttttc attgttgatc aggtattcca
    17881 catcgccatc gaaaggcaca ttcacttcga cctgaccatg ccagtggctg gtgggcatga
    17941 tatgcggtgc gcgaaactca atctccatcc gctggtattc cgaatacagc gacagcgggc
    18001 tgcgggtctg tttttcgtcg ctgctgcaca taaacgtatc tgtattcatg gatggctctc
    18061 tttcctagaa tatcagaatt atggcaggag tgagggagga tgactgcgag tgggagcacg
    18121 gttttcaccc tcttcccaga ggggcgaggg gactgtccga gcatcatgag gccgaaaact
    18181 ctacttttca ggtaatttat tcccataaac tcagatttac tgctgcttca cgcaggatct
    18241 gagtttatgg gaatgctcaa cctggaagcc ggaggttttc tgcagattcg cctgccatga
    18301 tgaagttatt caagcaagcc aggagatctg catgatgtct gcacccaaaa ttacatttat
    18361 cggcgctggt tcgacgattt tcgttaaaaa tattcttggt gatgtgttcc atcgcgaggc
    18421 gctgaaaacg gcgcatattg ccctgatgga cattgacccc acccgcctgg aagagtcgca
    18481 tattgtggtg cgtaagctga tggattcagc aggggccagc ggcaaaatca cctgccacac
    18541 ccaacagaaa gaagccttag aggatgccga ttttgtcgtg gtggcatttc agattggcgg
    18601 ttatgaacct tgcacggtga ctgatttcga ggtctgtaag cggcatggtc tggaacaaac
    18661 cattgccgat acgttggggc cgggcggtat tatgcgcgcg ctacgtacca ttccgcatct
    18721 gtggcaaatt tgcgagaaca tgacggaagt ctgccccgat gccaccatgc tcaactatgt
    18781 taacccaatg gcgatgaata cctgggcgat gtatgcccgc tatccgtata tcaaacaggt
    18841 cgggctgtgc cattcggtgc agggaacggc ggaagagctg gcgcgtgacc tcaatatcga
    18901 cccggctacg ctgcgttacc gttgcgcagg tatcaaccat atggcgtttt acctggagct
    18961 ggagcgcaaa accgccgacg gcagttacgt gaatctctac ccggaactgc tggcggctta
    19021 tgaagcaggg caggcaccga agccaaatat tcatggtaat actcgctgcc agaatattgt
    19081 gcgctacgaa atgttcaaaa agctgggcta cttcgtcacg gaatcatcag aacattttgc
    19141 cgaatatacg ccgtggttta ttaagctagg tcgtgaggat ttgattgagc gttataaagt
    19201 accgctggat gagtacccga aacgctgcgt cgagcagctg gcgaactggc ataaagagct
    19261 ggaggagtat aaaaacgcct cccggattga tattaaaccg tcacgggaat atgccagcac
    19321 aatcatgaac gctatctgga ctggcgagcc gagtgtgatt tacggcaacg tccgtaacga
    19381 tggtttgatt gataacctgc cacaaggatg ttgcgtggaa gtagcctgtc tggttgatgc
    19441 taatggcatt cagccgacca aagtcggtac gctaccttcg catctggccg ccctgatgca
    19501 aaccaacatc aacgtacaga cgctgctgac cgaagctatt cttacggaaa atcgcgaccg
    19561 tgtttaccac gccgcgatga tggacccgca tactgccgcc gtgctgggca ttgacgaaat
    19621 atatgctctt gttgacgacc tgattgccgc ccacggcgac tggctgccaa gctggttgca
    19681 ccgttaaaac gcgactaaac gctactgcgc cgggggattt attccggcgc acacctctga
    19741 cgataccaat aacagaaggc gggcgttggt aacagcgacc cgatacccta tgagcatttc
    19801 aatgactaca aaactcagtt atggatttgg agcgttcggg aaggattttg cgatcggcat
    19861 tgtgtatatg tacctcatgt attactacac cgatgtcgtc gggctgtctg tgggtttggt
    19921 cggtactttg tttctggtgg cgaggatctg ggatgctatt aacgatccga ttatgggatg
    19981 gattgtaaat gctacgcgat cgcgatgggg taagttcaaa ccctggatcc tgatcggtac
    20041 gttggcaaac tctgtaatct tatttctcct ctttagtgcg catctgtttg aaggtactac
    20101 tcagattgtc tttgtttgcg tgacctacat cctctggggc atgacttaca ccattatgga
    20161 tattcccttc tggtcgctgg ttccaaccat cacgctcgat aaacgtgaac gcgaacaact
    20221 ggttccttat ccgcgttttt ttgccagtct ggcaggcttt gttacggcag gtgtgacgct
    20281 accatttgtt aattatgtcg gcggtggcga tcggggattt ggctttcaga tgttcactct
    20341 ggtattgatc gcctttttta ttgtttcaac catcatcact ctgcgcaatg tgcatgaagt
    20401 cttttcgtca gacaatcaat cgtctgctga aggaagccat ctgacactta aagccatcgt
    20461 tgcgcttatt tataaaaacg atcagctttc atgcctcttg ggtatggctc ttgcttataa
    20521 tgtagccagc aacattatta ccggatttgc tatctattat ttctcatatg ttatcggtga
    20581 tgcggatttg ttcccctatt atctgtcgta tgcgggagct gctaacctgg tgacgttagt
    20641 attcttccca cgcttagtta aatcattatc ccgacgcatt ttatgggccg gagcatctat
    20701 tcttccggtg ttaagctgtg gtgttctcct gttaatggca ttaatgagct atcacaacgt
    20761 cgtcctcatt gtgattgcgg gtattttgct gaatgtggga acggcgcttt tctgggtatt
    20821 acaggtcatc atggtggcag ataccgttga ttacggtgaa tataaactgc acgtacgctg
    20881 tgaaagcatc gcttactccg tgcagactat ggtggtgaag ggcggttcag cctttgcggc
    20941 ttttttcatt gcggttgtgt tagggatgat tggctatgta ccgaatgttg aacagtctac
    21001 gcaagcccta ttaggtatgc agtttattat gattgctcta ccgactctgt ttttcatggt
    21061 aacgctgatt ctctacttcc gtttctatcg cctcaatggc gacacgctgc gcaggatcca
    21121 gattcatctg ctggataaat atcgcaaagt accgcccgag cctgttcatg ctgatattcc
    21181 ggtcggtgca gtgagtgatg tgaaagcctg acgtggacaa tagctaacga aaatggcctg
    21241 atgcgatatg tttatcaggc cacgtcttaa tgtaatgctt tggatttgca aattttgtag
    21301 accggataag gaattcacgc cgcatccggc atcaacaaag tgcacgttgt tatccagtta
    21361 tcaagccata gcgccgtagc tggcggcaat gaaagcgagc gagcaatatc actgtcgtta
    21421 atgcgccaag cagcgcgcag aacatatcgg attgcgtgtc ccactggtcg ccctgggtac
    21481 cgagaaaatc atccgctccc tggcccatcg ccagtgccgc ccaccactcg attaattcat
    21541 acatcgcgct tatcgccagc gccacgcaac agaccagaaa cgccaccatt ttacgtccgc
    21601 gcacgtacat cccgcgaacg agaatttctc gtgccaccag tgcaggcacc agcccctgga
    21661 aaaagtgccc cagcttgtca tacggattac gactcaaccc cagccattcc tgcacctcaa
    21721 aaccaacggg gactttcgcg taggtgtact gcccgcctac catcaggatg atggcgtgaa
    21781 agaagatgag cgtatagagc agcagcgtta acgggtaacg tctggcggtg gcaagtagta
    21841 gcggcacgac gataatcacc ggtgtcactt ccagcagcca ggtgagtttg tcatgggccg
    21901 aaatgccggt ataaatcagg attaacgtta gcgccagtgc gccagtgtta agaattaacg
    21961 gcttgagtgt gcgggtcatg gtgagttcgc aagtcaggga aaatactgac tattcaccag
    22021 caagcatgaa attacaatcg caaaagaggt ggcccagggg gatcacctgg cagcatgctg
    22081 ccaggcgctg ggccgaagag gttacttagt gcagttcgcg cactgtttgt tgacgatttg
    22141 ctggaagaag tcgttacctt tgtcatcgac caggataaac gccgggaaat cttctacttc
    22201 gattttccag atagcttcca tacccagctc cggataagcg acgcattcca gatgcttgat
    22261 gctctgctgc gccagtaccg ccgccggacc gccgatgcta ccgaggtaga agccgccgtg
    22321 tttatgacac gcgtcggtaa cctgctggct gcggttgcct ttcgccagca tgatcatgct
    22381 gccgccgtgg gattgcagca gatccacata ggagtccata cgacctgcgg tggttgggcc
    22441 aagtgaacct gatggataac cggcaggggt tttcgccgga cccgcgtagt agatcgggtg
    22501 atctttgatg tactgcggaa gttctttacc ggcgtcaatc agctctttca gcttggcgtg
    22561 tgcaatatct cgacccacaa taatggtgcc agtgagcgac aaacgagtgg aaaccggata
    22621 ttgcgaaagc tgggcgagga tctctttcat cgggcggtta aggtcgacct tcaccgcttc
    22681 gccttcaccg gcctgacgca gttcttgtgg aatgtactga cctagattgt gttccagttt
    22741 ttcgatccag ataccttcgc ggttgatttt cgctttaatg ttacggtcag cggagcagga
    22801 gacgcccatg cctaccgggc aggatgcgcc gtgacgtggc agacggataa cgcgaatgtc
    22861 gtgcgcgaag tatttaccgc caaactgcgc gccaagaccg agtttctggg cctcttccag
    22921 cagttcctgt tccagctgga catcgcggaa cgcctgacca tgttcgttcc cttccgtcgg
    22981 cagttcatcg taatagtgag cgcttgctaa tttgacggtt ttcaggttgt tttccgcaga
    23041 cgtaccgcca atcacaaacg cgatatggta cggcgggcag gctgcagtac cgagggtacg
    23101 cattttctcg acgaggaagt ttttcagttt gccgggagtc agcagggctt tggtttcctg
    23161 gtagagatac gttttgttgg cagagccgcc gcctttcgca acgcaaagga atttgtactc
    23221 atcgccatct accgcgtaca ggtcgatttg cgcaggcagg ttagtgccgg tgttgacctc
    23281 tttgtacatg tccagcgccg cgttttgcga gtagcgcagg ttatcttcga tataggtgtt
    23341 atagacgcct ttcgacagcg cttcttcatc accgccaccg gtccacacgc gctggccttt
    23401 tttaccgacg atgatcgcgg tgccggtatc ctggcaggtc ggcagcacgc ctttggcggc
    23461 gatttcggag tttcttaaga attgcagtgc cacgtacttg tcgttttcgc tggcttctgg
    23521 atcgtgaaga atagccgcaa cctgtttctg gtgtgccgga cgtagcatga aagaagcgtc
    23581 gtgaaaggct tgctgcgcca gcagggtcag ggcttctggt tccactttca ggatggtttc
    23641 gccgtcgaag tcggcaacgc taacgtaatc ggaagtgagt agatagtatt cggtattgtc
    23701 tttccccatc gggaaaggtg cctggtagat aaagggtttg tttgacatag cttccagcct
    23761 gtaactctgt atttgttatt cgaaaatggc gtgccgcgta gtcacgggcg gcacgcaaag
    23821 tgcatttata agaacccgta catcgcggcg aagatccagc cgaagacgca cgatacgctc
    23881 acaccaatca accccggcag aataaaactg tggttgatga cgaagcgacc gatgtgggtg
    23941 gtgccggaac ggtcaaactg aatcgctgcc agatcgctcg gataagtcgg caggatgtaa
    24001 taaccgtagc aagccggtgc tgaagccacg atgtatgccg gatcaacgcc gatcgccagc
    24061 gcgaccggaa caatcgccgc cagcgccgca gcctgagagt ttacaaactt ggaaaccagc
    24121 agcagaacaa tggcatacgc ccacggatac tctttcacca tttcacccag tacgccctga
    24181 atttcagaca tatgcgcacc gaacatggtt tctgccatcc atgcgatacc gtacaccgcc
    24241 acgatggcga tcataccgga acggaagact tcgttttttg agatagacgc gggattggtt
    24301 ttggtcagga taataatcag cgccccggtc agcagcataa acatctgaat aaccagtacc
    24361 atcgacagcg gtttgccgcc gaaggatgga cgcaggtccg aatcagcacc aagaagggcg
    24421 actacagcga ttgccccgag gaaaatccac attgccagcc agttgctttt cggcagtttt
    24481 ttatccagca gcgtcgcggt atcaccgtaa acatactcac ggttttccgg tacggagatg
    24541 aatttctgga actcttcgtc tttatccaga tctttaccgc ggaaccagct gaagataccg
    24601 atcgccagga taccgattaa cgtcgatgga atggtgattg ccagcagatc gaggaactca
    24661 agatggcgac catcaaaggt gacattaccc agcatcgcaa ccagagacac gaccgcaacc
    24721 gacaccggac tggcgataat ccccatctgt gcaccgatag aacttgccgc catcggacgt
    24781 tccggacgga tgttgttctt aatggcgacg tcgtagatga tcggcagaat ggtgtaaacc
    24841 acatgacccg taccgcaaag aatggtcagt gtacaggtca caaacggcgc gacaattgag
    24901 acatatttcg ggttgcggcg cagcagcttc tcggcaattt gcagcatgac atcaagaccg
    24961 cccgaagctt gcaaggtcgc cgatgccgcc accaccgcaa tgataaccag catgacatca
    25021 actggtggtt tacctggctg aaggtggaag acgaagacca gaatgaccag accgataccg
    25081 cctaataaac ccagcgcgat accacccttt ctggcaccat aaaacagaca tattagtatt
    25141 atgataagtt ggatagtaaa taacatgtgt gaaccctcgc gataatccta tttaaatttt
    25201 tgctgaatag atcacagtca cgttctgttt tgtatgaact gttttcagag cctgactaaa
    25261 tatccgtttg gtcgttaccg gctttagcaa atacctcaca gtgaatattg gctgattaaa
    25321 gcagcggcaa atttatgcac tgtgataagc ggctttttca gagagaaaaa ctctctggcg
    25381 atgggtttat agtttgtact gcgcagatgt taatgttttg tttgctgtta tcttattgat
    25441 atttatcggt ctattttttg ttctcagggc agatatcatt aggtttatag attaatctga
    25501 tctacctatt tgtgggtaaa aatacacata atgcgggtga cataatagtt aattaacttt
    25561 tgttatcgtt ttgaaattaa aaacaccgtt catctgaaga gatattaatt tttaacgatg
    25621 atggagggat aattatattt gatctggcac aagttttact gattaacgat gtaacttgtg
    25681 ccaggggtat tttgcattac ggtaataatt attacttaca gataaccagc gatccgttat
    25741 tggcaatatt gtttcagcag tgagtagtgt tctgcctgaa tacggtaacg gtaaaccgga
    25801 cgccccgtga cgccataatg gatactggtg aacaagatgt ggcagttgac cagccagatg
    25861 aggtattttc ggcaggaaac acgcgagatg ttaacctcgt tagccagctc gtcggttgaa
    25921 aactcatagt cctgatgcgc gtcaatccac tggcacagcg tgcgtaacgt ctgcggcgtt
    25981 aagccttttg gcaagcgacg aggatcctgt tcgttggagc tgctgccgtg gattagctga
    26041 tcaagctcgg cctggtcata atactgatgt ttttccagcg ccattttctt ttgccgccag
    26101 ccggtgagcg cctcttcaaa gcgggaagcc tggaagggtt tgatcaggta atccacgaca
    26161 ccgtaatgca gcgaatcttt aatggttgcc gcatcggctg cggaggagat gacaatcaca
    26221 tcacttttgc aacgcgcgtt atgcaggaca ggcagtaaat cgagcccgtt ctctttttgc
    26281 atatagatat cgagcaatat caggtcgata ggcgcatcgc tattgaagat aatctctttg
    26341 gctttctcca gcgtcgaggc tgttccacag cattgaaagc ctgggatttg tgctacgtat
    26401 cggcgattca gctccgcgac cattgcgtcg tcatcgataa ttaatacatt gatcatctgt
    26461 tcgacctctc cccgtcccag ggtatctgga caaaaaattg tgtgaaaatc cccggttccg
    26521 attccacggc gatgctgccg ccgagatttt ctacctgttg tttgacaagt gctaaaccga
    26581 cgcctcgctc gcttcctttt gtcgagacac ctttgtcaaa aatgtgatcg attttgtcgg
    26641 gtgcgatccc cggtccatca tcgttaactt cgcagtgcag ccagccgtga cggtagtgca
    26701 atgttacgct aatttcgcca ccgggttccg gccctaatgc ttccagcgcg ttttctatca
    26761 gatttcccaa tgtggtaatc agcgtcgcga cctggtcctc actgccgctg tctggcagct
    26821 ggctttcact gtttaaaatc agcgtatggc ctaaatcggt cgcgcggtta atcttgctga
    26881 ttaaaaaacc agcgataacc ggagatttga tcttacccag cagagagcca atctcttcct
    26941 gatagttatt ggctgttttg agaatgtaat cttccaactg cttataactc ttcagatgca
    27001 ataatccgag aatcacatgc aatttattca taaattcgtg ggatcgttca cgaagtgcgt
    27061 cagcatagtt gaccagaccg tcgagtcgct gcatcagttt acgaacttca gttttgtccc
    27121 tgaaggttga aatggcaccg atgataacgc cattactgcg caccggaacg gtgttgatca
    27181 gtaatagccg gtctttaatc gtaatctctt cgtcgcggcg cggcgtaccg tcgcgtaaca
    27241 cttccgagac atctaccacc tgtgaccatg agtggctgag cgtcgacagt ttctcatcgt
    27301 cctgcgactt acggtaattc agcaattctt gtgcggcatc gttgatcagc gtgacctcgc
    27361 cgcgatcgtc cacggcaacg acgccttctt tgatagactg caacatggcc tggcgttgct
    27421 caaacagcgt ggagatttcg tagggttcca ggccgaaaag gatttttttc agtaccttaa
    27481 ccagaatgca ggtgccaatc agtccgacca gcatgccaaa taataccgac cagataatgc
    27541 tccagcgact gtcattgatc tgttgggtca cgcggcttaa ctcaaggccg atcgccacca
    27601 cgccaatttg tttatgattt tcatcgtaga tgggggtaaa tacgcgtaaa gcctgcgcca
    27661 gaaaaccgcg attgatagcg acattttctt cgccattcag cgcattaagg atgtcatcac
    27721 ctttaaatgg ctgaccaata cgctgggctt caggatgtga gtagcgaaga ctttgcatat
    27781 cggtaacgac aataaacagc agatcgttgc gtttgcgcac ggcttccgcg atggcctgga
    27841 tgccactctc ctgcggtttt ttctgcaagc cctgacggat ttccggcgag tcggcgaggg
    27901 tacgcgccac tgccagtgcc ttgttggcca gcccgtctcg cgtcatatca ctgatttgcg
    27961 agaagtaaat cagatgcacc accaatagca ccgagaacag taccgcgctg accattaaga
    28021 tcacagtggt actcaatttc atcggacgtt tgcgtaatat gtggtagggc aatgaatgtc
    28081 tcatcagctt ccttgtgtga caaatttctt aagcattatc tctgatgagg cgggtaattc
    28141 aaagggagta aaaatgattg gctatatagg ggaagagact ctggcaacgg aaactgccag
    28201 tgctgtatga agattccggg gctatgctta tagcgataat catactgatg agagagggaa
    28261 ggtcatggat caggcgctac tggacggggg ttatcgctgt tataccggcg aaaagatcga
    28321 tgtctatttc aacactgcga tatgtcagca ttctggcaat tgcgtacgtg gcaacggcaa
    28381 gttatttaat ctcaaacgaa agtcgt
//
LOCUS       DAZXUL010000050        28195 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZXUL010000050 DAZXUL010000000
VERSION     DAZXUL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28195
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.050"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..209)
                     /locus_tag="ACH3L1_002120"
     CDS             complement(<1..209)
                     /locus_tag="ACH3L1_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFT4076847.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACH3L1_002121"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACH3L1_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFT4076848.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACH3L1_002122"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACH3L1_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFT4076849.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACH3L1_002123"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACH3L1_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFT4076850.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACH3L1_002124"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACH3L1_002124"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFT4076851.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACH3L1_002125"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACH3L1_002125"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFT4076852.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACH3L1_002126"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACH3L1_002126"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFT4076853.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACH3L1_002127"
     CDS             complement(4598..5320)
                     /locus_tag="ACH3L1_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFT4076854.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACH3L1_002128"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACH3L1_002128"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFT4076855.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACH3L1_002129"
     CDS             6520..6738
                     /locus_tag="ACH3L1_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFT4076856.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACH3L1_002130"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACH3L1_002130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFT4076857.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACH3L1_002131"
     CDS             complement(7472..7672)
                     /locus_tag="ACH3L1_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFT4076858.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACH3L1_002132"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACH3L1_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACH3L1_002133"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACH3L1_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFT4076859.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="ACH3L1_002134"
     CDS             10101..12014
                     /locus_tag="ACH3L1_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFT4076860.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="ACH3L1_002135"
     CDS             12014..13036
                     /locus_tag="ACH3L1_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFT4076861.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="ACH3L1_002136"
     CDS             13030..13248
                     /locus_tag="ACH3L1_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFT4076862.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="ACH3L1_002137"
     CDS             13302..14171
                     /locus_tag="ACH3L1_002137"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFT4076863.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="ACH3L1_002138"
     CDS             complement(14226..14630)
                     /locus_tag="ACH3L1_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFT4076864.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="ACH3L1_002139"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="ACH3L1_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFT4076865.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="ACH3L1_002140"
     CDS             15615..17705
                     /locus_tag="ACH3L1_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFT4076866.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACH3L1_002141"
                     /gene_synonym="dapC"
     CDS             complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACH3L1_002141"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFT4076867.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACH3L1_002142"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACH3L1_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFT4076868.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="ACH3L1_002143"
     CDS             complement(19673..20275)
                     /locus_tag="ACH3L1_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFT4076869.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="ACH3L1_002144"
     CDS             complement(20265..20432)
                     /locus_tag="ACH3L1_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFT4076870.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACH3L1_002145"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACH3L1_002145"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFT4076871.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="ACH3L1_002146"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="ACH3L1_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFT4076872.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="ACH3L1_002147"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="ACH3L1_002147"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFT4076873.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="ACH3L1_002148"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="ACH3L1_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFT4076874.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="ACH3L1_002149"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="ACH3L1_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFT4076875.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cysG"
                     /locus_tag="ACH3L1_002150"
     CDS             26649..28022
                     /gene="cysG"
                     /locus_tag="ACH3L1_002150"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFT4076876.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6846 a         7536 c         7101 g         6712 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgt
//
LOCUS       DAZXUL010000051        28021 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZXUL010000051 DAZXUL010000000
VERSION     DAZXUL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28021
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.051"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            516..2537
                     /gene="uvrB"
                     /locus_tag="ACH3L1_002151"
     CDS             516..2537
                     /gene="uvrB"
                     /locus_tag="ACH3L1_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFT4076877.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(2729..3637)
                     /gene="yvcK"
                     /locus_tag="ACH3L1_002152"
     CDS             complement(2729..3637)
                     /gene="yvcK"
                     /locus_tag="ACH3L1_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFT4076878.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      3902..4046
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4034..5023
                     /gene="moaA"
                     /locus_tag="ACH3L1_002153"
     CDS             4034..5023
                     /gene="moaA"
                     /locus_tag="ACH3L1_002153"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFT4076879.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            5045..5557
                     /gene="moaB"
                     /locus_tag="ACH3L1_002154"
     CDS             5045..5557
                     /gene="moaB"
                     /locus_tag="ACH3L1_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFT4076880.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            5560..6045
                     /gene="moaC"
                     /locus_tag="ACH3L1_002155"
     CDS             5560..6045
                     /gene="moaC"
                     /locus_tag="ACH3L1_002155"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFT4076881.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            6038..6283
                     /gene="moaD"
                     /locus_tag="ACH3L1_002156"
     CDS             6038..6283
                     /gene="moaD"
                     /locus_tag="ACH3L1_002156"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFT4076882.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            6285..6737
                     /gene="moaE"
                     /locus_tag="ACH3L1_002157"
     CDS             6285..6737
                     /gene="moaE"
                     /locus_tag="ACH3L1_002157"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFT4076883.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            6874..7578
                     /locus_tag="ACH3L1_002158"
     CDS             6874..7578
                     /locus_tag="ACH3L1_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFT4076884.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            7783..8496
                     /locus_tag="ACH3L1_002159"
     CDS             7783..8496
                     /locus_tag="ACH3L1_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFT4076885.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(8532..9488)
                     /locus_tag="ACH3L1_002160"
     CDS             complement(8532..9488)
                     /locus_tag="ACH3L1_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFT4076886.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(9488..10729)
                     /gene="clsB"
                     /locus_tag="ACH3L1_002161"
     CDS             complement(9488..10729)
                     /gene="clsB"
                     /locus_tag="ACH3L1_002161"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFT4076887.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(10726..11487)
                     /locus_tag="ACH3L1_002162"
     CDS             complement(10726..11487)
                     /locus_tag="ACH3L1_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFT4076888.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            11620..12030
                     /locus_tag="ACH3L1_002163"
     CDS             11620..12030
                     /locus_tag="ACH3L1_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFT4076889.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(11992..13098)
                     /locus_tag="ACH3L1_002164"
     CDS             complement(11992..13098)
                     /locus_tag="ACH3L1_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4076890.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(13109..14242)
                     /locus_tag="ACH3L1_002165"
     CDS             complement(13109..14242)
                     /locus_tag="ACH3L1_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4076891.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(14235..15971)
                     /locus_tag="ACH3L1_002166"
     CDS             complement(14235..15971)
                     /locus_tag="ACH3L1_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFT4076892.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(15964..16959)
                     /gene="hlyD"
                     /locus_tag="ACH3L1_002167"
     CDS             complement(15964..16959)
                     /gene="hlyD"
                     /locus_tag="ACH3L1_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFT4076893.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(16962..17633)
                     /gene="cecR"
                     /locus_tag="ACH3L1_002168"
     CDS             complement(16962..17633)
                     /gene="cecR"
                     /locus_tag="ACH3L1_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFT4076894.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            17862..19082
                     /gene="rhlE"
                     /locus_tag="ACH3L1_002169"
     CDS             17862..19082
                     /gene="rhlE"
                     /locus_tag="ACH3L1_002169"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFT4076895.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(19076..19174)
                     /locus_tag="ACH3L1_002170"
                     /pseudo
     CDS             complement(19076..19174)
                     /locus_tag="ACH3L1_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19256..19738)
                     /gene="ybiA"
                     /locus_tag="ACH3L1_002171"
     CDS             complement(19256..19738)
                     /gene="ybiA"
                     /locus_tag="ACH3L1_002171"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFT4076896.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            19858..22008
                     /gene="dinG"
                     /locus_tag="ACH3L1_002172"
     CDS             19858..22008
                     /gene="dinG"
                     /locus_tag="ACH3L1_002172"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFT4076897.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            22036..22998
                     /gene="ybiB"
                     /locus_tag="ACH3L1_002173"
     CDS             22036..22998
                     /gene="ybiB"
                     /locus_tag="ACH3L1_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFT4076898.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            23139..24224
                     /gene="hcxB"
                     /locus_tag="ACH3L1_002174"
     CDS             23139..24224
                     /gene="hcxB"
                     /locus_tag="ACH3L1_002174"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFT4076899.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            24291..24350
                     /gene="ybiE"
                     /locus_tag="ACH3L1_002175"
     CDS             24291..24350
                     /gene="ybiE"
                     /locus_tag="ACH3L1_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFT4076900.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(24453..24713)
                     /gene="ybiJ"
                     /locus_tag="ACH3L1_002176"
     CDS             complement(24453..24713)
                     /gene="ybiJ"
                     /locus_tag="ACH3L1_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFT4076901.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(24978..25244)
                     /gene="ybiI"
                     /locus_tag="ACH3L1_002177"
     CDS             complement(24978..25244)
                     /gene="ybiI"
                     /locus_tag="ACH3L1_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFT4076902.1"
                     /translation="MASAWANDDAVNEQINSTTEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(25318..25995)
                     /gene="ybiX"
                     /locus_tag="ACH3L1_002178"
     CDS             complement(25318..25995)
                     /gene="ybiX"
                     /locus_tag="ACH3L1_002178"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFT4076903.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(26037..28021)
                     /locus_tag="ACH3L1_002179"
                     /pseudo
     CDS             complement(26037..28021)
                     /locus_tag="ACH3L1_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         6610 a         7222 c         7515 g         6674 t
ORIGIN      
        1 ttaaattcta aaataatcac gaaaaaaatt ttacttccgc ctcatgcggc gaatgtggga
       61 attgcccagg cggcggggga taggggctgg agacagttat ccactattcc tgtggataac
      121 catgtgtatt agagttagaa aacacgaggc aagcgagaga atacgcggct tgcacgcgaa
      181 ttggcgttaa agacggctca aagaaatatc ttttattttt taactggtta gataaatgca
      241 atggcagtca ctgaacaggc atctcttgcc ataaaactgt catcactcat cttgacaaat
      301 gttaaaaaag ccgttgcttt ggggataacc cggtaaggct ggagttttat ctcgccacag
      361 agtaaatttt gctcatgatt gacagcggag tttacgctgt atcagaaata ttatggtgat
      421 gaactgtttt tttatccagt ataatttgtt ggcataatta agtacgacga gtaaaattac
      481 atacctgccc gcccaactcc ttcaggtagc gactcatgag taaaccgttc aaactgaatt
      541 ccgcttttaa accttctggc gatcagccag aggcgattcg acgtctcgaa gaggggctgg
      601 aagatggcct ggcgcaccag acgttacttg gcgtgactgg ctccgggaaa accttcacca
      661 ttgccaatgt cattgctgac cttcagcgcc caaccatggt acttgcgccc aacaaaacgc
      721 tggcggccca gctgtatggc gaaatgaaag agttcttccc ggaaaacgcg gtggagtatt
      781 tcgtctccta ctacgactac tatcagccgg aagcctatgt accgagttcc gacaccttca
      841 ttgagaaaga tgcctcggtt aacgaacata ttgagcagat gcgtttgtcc gccaccaaag
      901 cgatgctgga gcggcgtgat gtgattgtag tggcgtctgt ttccgcgatt tatggtctgg
      961 gcgatcctga tttatatctc aagatgatgc tccatctcac ggtcggtatg attatcgatc
     1021 agcgcgcgat tctgcgccga ctggcggagc tgcaatacgc tcgtaatgat caagcattcc
     1081 agcgtggtac tttccgcgtt cgtggcgagg tgatagatat cttcccggca gaatcggatg
     1141 acattgcact tcgcgtggaa ctgtttgacg aggaagtgga acgattgtcg ttatttgacc
     1201 cgctgaccgg gcagattgtt tccactattc cacgttttac catctacccg aaaacgcact
     1261 acgtcacacc gcgcgagcgc atcgtacagg cgatggagga gatcaaagaa gaactggccg
     1321 ccagacgcaa agtgctgttg gaaaacaaca aactgctgga agagcagcgg ctgacccagc
     1381 gtacccagtt tgatctggag atgatggacg agctgggcta ctgttcgggg attgaaaact
     1441 actcgcgctt cctctccggt cgtggaccgg gtgagccacc gccgacgctg tttgattacc
     1501 tgcctgccga tgggctgctg gtcgtcgatg aatctcacgt caccattcca caaattggcg
     1561 gcatgtatcg cggtgaccgg gcgcgtaaag agacactggt ggagtacggc ttccgcctgc
     1621 catcagcgct ggataaccgt ccgcttaagt ttgaagagtt cgaagcatta gcgccgcaaa
     1681 ccatctatgt ttcggcgacg ccgggtaatt acgagctgga aaaatccggc ggcgatgtgg
     1741 tggatcaggt ggtgcgtcca accggattgc ttgacccgat tatcgaagtg cggccggtgg
     1801 cgacacaggt tgatgatctt ctttcggaga ttcgtcagcg agcggcaatt aacgaacgcg
     1861 tgctggtcac cacactgacc aagcgaatgg cggaagatct taccgaatat ctcgaagaac
     1921 acggtgagcg cgtgcgttat cttcactcag atatcgacac cgtcgagcgt atggagatta
     1981 tccgcgactt gcgtctgggt gagttcgacg tattggtagg gatcaactta ctgcgtgaag
     2041 gtctggatat gccggaagtg tcgctggtgg cgatcctcga cgctgacaaa gaaggcttcc
     2101 tgcgttccga acgttcgttg atccagacca ttggtcgtgc ggcacgtaac gttaacggta
     2161 aagcgattct ctacggcgat aggagcactc catcaatggc gaaagcgatt ggcgaaaccg
     2221 aacgtcgccg tgagaaacag cagaagtaca acgaggaaca cggcattacg ccgcaaggct
     2281 tgaacaagaa agtggtcgat atcctggcgc tggggcagaa cattgccaaa accaaagcga
     2341 agggcagagg aaaatcgcgc ccgattgttg agccggataa tgtgccgatg gatatgttgc
     2401 cgaaagcgtt gcagcagaaa atccatgaac tggaagggct gatgatgcaa cacgcgcaga
     2461 atctggagtt cgaagaagcg gcacaaattc gtgaccagtt gcatcagttg cgtgagttgt
     2521 ttattgccgc atcgtaacag gatagcgaag aagactgatg acaaacggaa aacagtctga
     2581 tgcgctacgc ttatcaggcc tacattttct ccgcaatata ttgaatttgc gcggtttgta
     2641 ggccggataa ggcgttcacg ccgcatccgg cataaacaac gcgcactttg tcttcaatag
     2701 gaagccggaa ttttccttcg gatttccgtt aacctaaagc ctgtaacgcc ttttccagcg
     2761 cgttatgtaa caactggcgg tcatgacgat acggaatatc gctggcctcc agtacctcct
     2821 ggatcacaat ccgctctttc accgccgaga catccacttt tggcccgacg atgaccgcat
     2881 caatgacctt tttaccaaca tactgctcca taattgccag cttgctttcc agcttcaaat
     2941 tagccgcagg taaactcaac tcacgcccca gattgccgat ataaaccatc ggcgctggcg
     3001 tgcggcgtaa tgcctgggcg atttccttca gcagcagaat tggcatcagg ctggtataaa
     3061 aactgccagg cccaataatg atgagatccg cttcattgat agcgtgaacc gcctcacgcg
     3121 ttgcgggtac attaggcgtt aacaataact cttgaatcgg cgtagttaac tggtcgatat
     3181 tgacctcgcc gtaaacttca tgcccctgat cgtcaatcgc catcagatca acaggatgct
     3241 ctgacattgg aatcaaatgc gtatccactt tcagcagatt acgaattaaa ttgatggctt
     3301 ccagaggccg cacgctaagg tgatccagcg cctttaacat caagtttccg agattatgac
     3361 cggaaagttc gccattgccg ccaaaacggt attcaaacat cgcggaggcg acgttcggtt
     3421 ccgttatcag ctggttgagg cagttgcgca tatcgcccca agcaatgccg ccttctgaac
     3481 ggcgaatacg ccccgtcgag ccaccattat ccgtggtggt gacgataccc gttaaacgag
     3541 aacccaaaga cgaaagtgat gagagaacgc gtcccagtcc atgccctccg ccgagagcaa
     3601 cgacacgatc aagatcagcc agcgtacgat tgcgcatata gtttcctgga gtcagattat
     3661 ccgcgctaca gtagcgcaaa ttgcggggaa acagcaatta acctgccaat attgaggata
     3721 caaactattt tcttctatct ctaaaggacg atgcacgcta tgcctccctg atgatgtata
     3781 tcaaagtaaa accgccattt tcccttattc tgtagcgaaa tagcacgatc atgacgctat
     3841 atacatgatt acatagcgaa agtgtggatg gtaaaaatct catttacacg ctagtatcgg
     3901 cttaaccact aaacactcta gcctctgcac ctgggtcaac tgatacggtg ctttggccgt
     3961 gacaatgctc gtaaagattg ccaccagggc gaaggaagaa atgacttcgc ctcccgtatc
     4021 tggaaaggtg tacatggctt cacaactgac tgatgtattt gcgcgtaagt tttactactt
     4081 gcgcctgtcg attaccgatg tgtgtaactt tcgttgcacc tactgcctgc cggatggcta
     4141 caaaccgagc ggcgtcacca ataaaggctt tcttaccgtc gatgaaattc gccgggttac
     4201 gcgcgccttc gccagtctgg gcaccgaaaa agtgcgcctg acaggaggag agccgtcttt
     4261 acgccgcgac tttaccgata tcatcgccgc tgtgcgggaa aacgacgcta tccgccagat
     4321 tgcggtcacc accaatggtt accgtctgga acgcgatgtg gcgaactggc gcgatgcggg
     4381 acttactggc attaacgtca gtgtcgacag tttggacgcc cgccagtttc acgctattac
     4441 cgggcaggat aaattcaacc aggtcatggc agggattgat gctgcatttg aggccggttt
     4501 tgagaaggtc aaagtcaata ccgtgctgat gcgtgatgtt aatcatcacc agctcgacac
     4561 ctttctgaac tggatccagc atcgccctat ccagctgcgt ttcatcgaac tgatggaaac
     4621 gggcgagggc agcgagctct tccgtaagca tcacatctct ggtcaggttc tgcgtgacga
     4681 gctactgcgt cgcggctgga tccaccaatt acgtcaacgc agcgacggtc ccgcgcaagt
     4741 cttttgccat ccagattacg ccggagagat tggccttatc atgccgtatg aaaaagactt
     4801 ctgcgccact tgcaaccgcc tgcgcgtttc ctccattggt aaactccatc tctgcctgtt
     4861 tggtgaaggc ggcgttaacc tgcgcgatct gctggaaggc gatacccagc aacaggcgct
     4921 ggaagcgcgt atttcagcgg cgctgcggga gaagaaacag acccatttcc tgcatcaaaa
     4981 caacaccggt attacgcaaa acttatcgta cattggcggc taaaacgtca aaaggagaga
     5041 tcagatgagt caggtaagca ctgaatttat cccgacccgt attgctattc ttacggtttc
     5101 taatcgtcgc ggtgaagaag acgatacctc cggtcactat ctgcgcgatt cggcgcaaga
     5161 agcgggccat cacgttgtcg atgaagccat tgtgaaagaa aaccgctacg ctattcgcgc
     5221 tcaggtatct gcgtggatcg ccagcgacga tgtacaagtg gtattgatta cgggtggtac
     5281 tggcctgacg gaaggtgatc aggctcccga agcgctgctg ccgttgttcg accgtgaggt
     5341 tgaaggtttt ggtgaagtgt tccgtatgtt gtcgtttgaa gagattggca cttccacgtt
     5401 gcaatctcgt gcggtagcgg gcgtcgctaa caaaacgctg attttcgcca tgccgggttc
     5461 gaccaaagcg tgccgtaccg catgggaaaa tatcatcgcg ccgcagctgg atgcccgtac
     5521 gcgtccgtgt aatttccatc cacatttgaa gaaataagta tgtcgcaact gacccatatc
     5581 aacgccgctg gcgaagcgca catggtggat gtctccgcca aagcggaaac cgtgcgtgaa
     5641 gcgcgggcgg aagcctttgt caccatgcgc agcgagacgc tggcgatgat tattgatggt
     5701 cgccaccaca aaggcgacgt atttgccact gcgcgtattg ccggtattca ggcggcaaaa
     5761 cgcacttggg atctgatccc gctgtgtcac ccgctgatgc tcagcaaagt tgaagtcaat
     5821 ttacaggccg agccggagca caatcgggtg cgtatagaaa ccttatgccg cctgaccggg
     5881 aaaaccggtg tcgaaatgga agcgttaacc gcggcctccg tggcggcgct gaccatttat
     5941 gacatgtgca aagcggtgca aaaagatatg gtgattggtc cggtacgttt gctggcgaag
     6001 agcggcggca agtcgggtga ctttaaggtg gaagcggatg attaaagttc tttttttcgc
     6061 ccaggtgcgc gagttggtgg gaacagatgc aaccgaagtg gctgcggatt acccaaccgt
     6121 tgaagcgtta cgccagcacc tggctgcgca gagcgatcgc tgggcgctgg cgctggaaga
     6181 tggcaaattg ctggctgccg tcaaccagac gctggtgagt tttgaccatc cgctgactga
     6241 cggcgacgaa gtagctttct tcccgccggt aaccggaggt taagatggca gaaaccaaaa
     6301 ttgttgtcgg cccgcagccg ttcagcgtag gagaagagta cccgtggctg gcggagcgtg
     6361 acgaagacgg tgcggtagtc accttcaccg gaaaggtgcg caaccataac ctgggcgaca
     6421 gcgtcaaagc attaaccctc gaacactatc cggggatgac tgaaaaagca ctggcagaaa
     6481 ttgttgatga agcgcgtaac cgctggccgc tggggcgcgt cacggtgatt caccgcatcg
     6541 gggaattatg gccgggcgat gaaatcgttt ttgtcggtgt caccagtgcg catcgcagca
     6601 gtgcgtttga agccgggcag tttattatgg attatctcaa aacccgcgca ccgttctgga
     6661 agcgcgaagc cacgccggaa ggcgaccgct gggttgaagc ccgggagagc gatctgcagg
     6721 cggcaaaacg ctggtagttt tttgttagcc ggataaggca ccgctgcgtc cggcaatttc
     6781 accgctcagt acatgcgttt acgctgcgtc gatgcacagc ctcatcattt tgcagtatcc
     6841 ttaagatatt ccttatatct tcaggagatc gtcatggaca gattcccacg ttctgattca
     6901 atcgtacaac cctgggctgg cttgcaaact tatatggcgc aagtctatgg ctggatgacc
     6961 gttggcttgt tgctgaccgc atttgttgcc tggtatgcgg ctaattccgc ggccgtgatg
     7021 gagctgttgt tcactaaccg tgtcttttta atcggtctga tcatcgcgca attagcattg
     7081 gttattgtgt tatcagcgat gattcaaaag ctgagcgcag gtgtaacgac gatgctcttt
     7141 atgctttatt cggcgctgac gggtcttacg ctttccagta tattcattgt ctataccgct
     7201 gcttctatcg ccagtacttt cgtcgttact gccgggatgt tcggcgcaat gagcctgtac
     7261 ggttacacca cgaagcgcga tttaagtggc tttggcaata tgctgtttat ggcgttaatc
     7321 ggcattgtgc tggcatcgct ggtcaacttc tggctgaaaa gcgaagcatt gatgtgggca
     7381 gttacctaca tcggcgtgat tgtctttgtc ggattgacgg cgtatgacac tcagaaattg
     7441 aaaaatatgg gtgagcagat tgatacccgc gacacgtcga acctgcgcaa atattccatt
     7501 cttggtgcgt taaccttgta tctggacttc atcaacctgt tcctgatgtt gttacggatc
     7561 ttcggcaacc gccgttaatt ctttttcacc atcactatcc cggccatgtg tcgggatttt
     7621 tttcttacta aaaacgacac ttaacaagta ccaggtataa aatatgcctt atgagcgagc
     7681 ggaatcgttt tccgtatcca taaatacaaa agtattttgt atgcgtggtt ttaaatttat
     7741 tgatggtgaa tattaatgtt ttttctaagg acaactaaaa gaatggaatc atactcgcaa
     7801 aacagcaata aattagattt tcagcacgag gccaggatat taaacggtat atggctcatt
     7861 accgctttag gtttggtggc aaccgcagga ctagcctggg gagctaagta tctcgaaatt
     7921 acggcaacca aatatgattc accaccaatg tatgtcgcca tagggttatt attactttgt
     7981 atgtatggct taagtaagga tatcaacaag ataaatgccg ccatcgcggg cgtaatatat
     8041 ctgtttttac tctctttggt ggcgattgtc gttgcaagtt tagttcctgt atatgccatt
     8101 atcatcgtgt tcagcactgc gggcgcgatg tttttaatca gtatgctggc cggtttatta
     8161 tttaatgttg atcctggttc tcaccgtttt atcattatga tgacgttgac agggttggcc
     8221 ctggtaatca tcgtgaatgc ggcattaatg agtgaacggc ccatttgggt aataagttgc
     8281 ttaatgattg tgttatggcc aggcattatc tcgcatggac gaaataagct ccttgaactg
     8341 gcaggaaaat gccatagtga agagttgtgg agtccggttc gttgcgcttt tacaggtgca
     8401 ttaacactct attactattt tatcggcttc tttgggatac ttgccgcgat agctataacg
     8461 cttgtctggc aaaggcatac gcgttttttt cattagtcgt aaatgcagat actaaagaac
     8521 ttaactaaac ttcacatcgc cgcttcattt ttcgcccgta gcttcttcgc ttggctttcc
     8581 agtagcaggt aacagatcag cgccagcagc agcgggataa agtaatacag gacgcgataa
     8641 gcgagcaggg cggcgataat gctccctttc gaggtatgct ccccagccag tagtgcgata
     8701 aacaccgctt ccagcacacc gatccccgcc ggaatatgca cgatgacgcc agcaatacta
     8761 ctaaccagta acacgcccag tacaaagaaa tagttcacgc tttgaccaag taacagccag
     8821 ataatcgccc ccattaccat ccagttaacg ctggaaatca gcatctgagc gagggcgaat
     8881 ttccatgaag gcagcaccag cttttgccct ttgatggtca tatggcggtg cttcgcgaaa
     8941 gcgcaaaacc acaaataaac cgcgataatc atcagtaagc caatgccgag aatacgcagc
     9001 gtagtttgat cgacatacca gtaatccggc aactccacca cgcctgcggt aaagataatc
     9061 cctgccagta aaatatagcc cagccagttg gtggtaatac tgagcgagga aatccgcgta
     9121 atagtgctgc ccggtaaccc tagtcgggag tacaaacgat agcgcatacc aatgccgccg
     9181 acccaggtac tgagcgtcag gttgaaggcg tagcagataa acgacaccag catcacctgg
     9241 cgcttcgcca gtttgtgacc gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc
     9301 agatagctga cgaccaccag cccgaccgca ctaagcagcg caacgcgatt gtagtcgcgg
     9361 atgaccttcc agacctcttc ccagtccact tttttggcgt agaccaccag caacacaatc
     9421 accgcgataa aaaacagcca ggtgaggatc ttctttgcta agcgccagcg cgggtgtgat
     9481 ttactcatca ggggtttacc cccgtgtttt cagtttctac ccgatcctgc gtttccattg
     9541 tcggttgtgc ggggggatca acctgcgtca gacgtggtgt gtgtgccgga agccagccaa
     9601 ccagcgccgg gaagtggcgt aaaaagtgga acgccagcac gcttttggtc aggttccacc
     9661 aggtgcgttt gggcagcatg gtttcatcca cctgctgaca atctgcggca ataatgccgt
     9721 tcagattatc gcgcagcgtc tggttaaaat gacgatcgtg gatgatgaca tttgcttcga
     9781 gattcagtga caaactgagc ggatcgagat tactggaccc tactgtcgcc cagtgatcgt
     9841 ccatcaatgc cactttgccg tggagcgggc ggcggcggta ctcaaaaacc tgaacgccgc
     9901 ctttaaccag atagttatac agcaagcgcg caccgactct gacaatcggc atatccggtt
     9961 cgccctgaat gatcagtttg atccgcaccc cgcgccgtgc cgctttacgc aaggcgtgta
    10021 aaaatcgata gccggggaag aagtaggcgt tggcgataat cacttcccgc tgcgcctgag
    10081 tgagcatttt cagataatgg cgttcaatat catcgcgatg ttcttcgtta tcgcgccaga
    10141 ccagcaatac ctgcgcttct cccggctggc ggttctcttc cgctttgtga tgacgtcgcc
    10201 accagcgtcg tgccgcgctc tgtccaggca ggttttccag ctcaaactgg agaatatctt
    10261 cgacaatcgg cccttcaagg cgtaccgcgt aatcctgttt agcctctgga ccgtagctgg
    10321 acatatgctc ggcggagtaa ttcagcccgc caataaaggc gatacgcgcg tcgatcacca
    10381 caattttgcg atgcatccgg cgaaacacat tggtgcgcat accaaaaagg cgagggcggg
    10441 gatcgtagta gcggaacact acgccagctg ccgtcagttc attgacaaac tcatcgctga
    10501 gatccggcga accgtagcca tccagcaaga cttccgcttt aaccccgcgt tgcgctgctg
    10561 ccagtagtgc cgcatgcagc tgtttgccga cgttatcctc aaaccagata aacgtttcaa
    10621 gaatgatgcg ttcttgtgcc tcgccaatcg ccttaaacac cgcgggataa tattgctcgc
    10681 cgttttccag caactggatc ttattgcctt cgcgccagct acatttcata aatgaatctc
    10741 cgcacttaaa ggggcatgat cagaaaggtg tcgccatgtc cgcagcggca acgcggttgg
    10801 cgcgctggcg ctggcatttt tgacgtagat cctgtccagt cgtagtagag gaaattgcac
    10861 cggaaacgtg cgcgccgggc gtccgtgggc gcgggtaaaa atctcatcca gtccggcctg
    10921 cacttttaac ggatgattag ctttttgccg ccagtcattg aaatcacccg ccaccaatac
    10981 cggttcgccg tccggtagct cattcaccca ttcggcgagc atcgcaagct gcgcctgacg
    11041 gtgcgcctca cgcaggccca gatgtacgca catcacatga atcgcttttc cggtcatcgg
    11101 cggcacaatg cggcagtaga gcacgccgcg cttttccgca ccatcgaccg aaacatcgcg
    11161 attctcataa tgttcaatgg gataacgcga cagtacggcg ttgccgtgat gcccttccgg
    11221 gtatacggca ttgcgaccgt aggcaaaatc gctccacata gtgtcggcga gaaactcgta
    11281 gtgcgaggta tcgggccagt tttccacatg cagcggatga acttcgtgcg cgcccatcac
    11341 ttcctgcagg caaacaatat cggcgctgac ggtacgcacg gcatcgcgaa gttccggcaa
    11401 aatgaagcgt cggttaaacg cggtaaagcc tttgtgaatg ttgatggtga gcaccttgaa
    11461 cgaaaattgt tgtgtttgat cgggcataat tttcctgtct ttgcctctta tctcattgaa
    11521 atagtgtagt cggcgtcaca aaaaggtgcg gtcttacgga attttccgta aagttcggta
    11581 ctctgagtaa gtagagataa attcttcagg agagaagcca tgaagtggca acaacgtgtt
    11641 cgtgtcgcaa cgggtctaag ttgctggcag attatgttgc atttactggt agtggcgctg
    11701 ctggtggtgg gctggatgag taagactctg gttcacgtcg gcgtgggatt atgcgcactg
    11761 tattgtgtca cggtagtgat gatgctggtg tttcagcgcc accccgagca acgctggcgt
    11821 gaggtggcag acgtgctgga agagctgacc acgacctggt attttggcgc agcgctgatt
    11881 gtgctgtggc tgttgtcccg cgtactggaa aacaactttt tgctggcaat tgcagggctg
    11941 gcgatccttg ctggcccggc ggtagtgtct ttgctggcga aagataagaa gttacatcac
    12001 cttacgtcta aacatcgcgt acgccgctga ccctgtcgtg gccgttatca ccagtagcgg
    12061 ccacaaacta ttccacacaa tatccagact cgcatccttc aaataaatct gcttggtaat
    12121 gtccgtaaag tggcgaatag ggttaatcca cgtcagattt tgcagccaca ctggcatgtt
    12181 ttccaccggc gaaacgtaac cggaaaggag aatggcgggc atcataaaaa caaacacgcc
    12241 gataaacgcc tgctgttgtg tcgagcagag tgatgaaatc aacagaccga atcccaccag
    12301 cgataaaccg taaatcacca tcgtaaagta gaacagcgct agcgatccgg cgaaggggat
    12361 ttgatacgcc cagataccaa tcgccagcac aatggtggcc tggaaggtgg cgacaattaa
    12421 cgccggtacg gctttgccga tgaagatctg ccaggtggtg agcggcgaaa ccagtagctg
    12481 atcgagcgta ccttgttcac gttcgcgggc gacggaaagt gaagtgacga tcattacgcc
    12541 gatagtggtg atcatggcga tcagcgacgg caccacaaac catttgtagt cgagattcgg
    12601 gttataccag ttgcgtacca ccagctcgct gttgttgggt ttcggttttc cttccagcag
    12661 ctcctgctga taatttttga cgatctgttg caggtagttg gcggcaattt gcgcactgtt
    12721 ggagttacgc ccgtcgagga tcaactgcaa cggcgcggtc tggaaggtat ccagtttgcg
    12781 cgagaagtca gccgggaaac gcaccagtag taacgccttt tgtgtgtcga tggtcgggcg
    12841 gatctcctgt gggcttttca gcagcagcac atgtgtaaag gcgctggcgc gggcaaaacg
    12901 ttgagtaagt tccaccgaat gctcgccgtt atcttcatcg tagatggcga tggtggcgtt
    12961 agtcacttcc agcgtggcgg cgaacgggaa caggatcacc tgaattagca cgggtaaaat
    13021 cagaatcgcg cgggtttgtg gttcgcgcag caacgactgc aactctttgc ggattaacgt
    13081 ccataagcga tgaaacatgc tcttctccct aatccagccg acgtttggtt ttcagccacg
    13141 tcaggccgat aaacatcacc gccgaagcga tcaaaaacag cacgtttacc accagcacca
    13201 ctggaatatt cccggcgagg aacaggcttt gcagggtgct gacgaaataa cgagcgggaa
    13261 taatgtacgt caccgcgcgg atcaccgcgg gcatactgtc gatctgaaaa ataaagccgg
    13321 aaagcataat cgacggcaga aaagcggcgt tcagggcgac ctgagcggca ttgaactggt
    13381 tgcgggtaat cgtggaaatc agcagcccca ttcccagggt actgagtaaa aacaggctgg
    13441 agataaaaaa cagaatcagc agcgaaccgc gatacggcac gccgagaata aacactgaca
    13501 ccagcataca cagcaacatc gccagcatcc cgagaaagta ataagggatc agcttacaca
    13561 gcagcagttc cgtgcgggta atctccgtag agagcagagc ctccatggtg ccgcgttccc
    13621 attctcgcgc caccaccagc gaggtgagaa tcgcgccgat gaccgtcatg ataatggtca
    13681 ccgcacccgg gataataaag tgctggctta tcgccgccgg gttaaaccag tagcgggttt
    13741 gtacatcaat aagcggttca aaagtctgcc cgttgtcctc cgctcgctgc atttgccaga
    13801 tctgccagat cccttcgaca tacccctgca caaagttagc ggtattcggc tcactgccgt
    13861 cggtgatcac ctgaatcggt gcggtggcgt tggcgcgctc catctgttcg gcgaaatcca
    13921 cgggaataac caccagaccg cgaattttcc ccgactgcat tttggcgatc agttcctgac
    13981 ggttatcgct gatggtggcg tcgatgtagg gcgaaccggt catggtgtgg gtgaaatcca
    14041 gcgccgcttc gctacgctgt tccagcaaaa tcccgacccg cagcttgctg gagtcgaggt
    14101 taatgccgta accaaaaata aacagcagta gcagtgggat cactaccgca atcagccagc
    14161 tactcggatc gcgaacgatc tgccgcgtct ctttgacgca cagcgcccgc acgcgatgcc
    14221 aggaaaggat cgggttactc attgctatgc tccttatccc agtcgtggat caactgaata
    14281 aaggcttgct ccatggtggg atcgggttgc tcatcgttag ctgactgtgc tttcaaatcg
    14341 tccggcgtac cgctggcgat taatttccca cggtacacca ggccgatgcg gtcgcaatat
    14401 tccgcttcat ccataaagtg ggtggtgacc atcaccgtga cgcctttctc taccatgctg
    14461 ttgatgtgca gccaaaattc acggcgggtg agggggtcaa cgccggaagt cggttcgtcg
    14521 agaaagagaa tatccggttc atgcatcagc gaacaggcca gcgccagccg ctgtttaaaa
    14581 cctaatggca gttcatcggt ggcgtgtgag gcaatacttt tcaggccaaa tgcctcgctc
    14641 atacgggaga ttttttcgtt ctgcgcccga ccgcgtaagc catacacacc agagaaaaag
    14701 cgtaaattct gttcgaccgt caggttgccg tagagcgaaa atttttgcgc catatagccg
    14761 agatgctggc gcgctttacc ggaactctct ttcagatcca tccccagcac cagcgcctgg
    14821 ccggaagtcg gcaccagcaa accgcacatc atcttaaagg tggtcgattt acccgcgccg
    14881 tttggcccca gcaaaccaaa aatctcccca cgtttaacgg caaagttgac gtgatcggtg
    14941 gcggcaaaat caccaaattt cttggtcagt tctttcgctt cgatcaccgt ctcgccgggt
    15001 gtgccttcta ccgtatgcaa tattgcaccc agcggcgatt ctgaggttcc ggcaccgccc
    15061 agcaaatcaa taaacgcatc ttcaaaacgc ggcgtagttt cgttgatgtt gatttccggc
    15121 atcccgtcgg catggcgaat atcgtctggt gtggcctctt ttttgaggat cagacgtacc
    15181 gatttcccct gaatcatgcc atcgctgacc tgcggcagtt tcaaggcgcg ttgcaacagt
    15241 ttgcggttgc cctcgtgtgg actggtcatc agaaagctgc gtccggccat ggtttgtgtc
    15301 agggcttttg gttctccctg atacagcaac tcgccttcgt tcatcagtaa cacgtcacgg
    15361 cactgctcgg cttcgtcgag atacgaggta ctccagagga ttaacatccc ttcgcccgcc
    15421 agctcatgca ccatctgcca cagttcgcgc cgtgagatag ggtcaacgcc gacgccgggt
    15481 tcatcgagca gcaacacttt cggttcgccc accagggtac aggccaggcc gagtttttgt
    15541 ttcatcccac cggagagctt gcccgccagg cgtccggtaa acggcccaag agacgtaaac
    15601 tccagcaggc gagcaaaagt ttgcttacgt gcctcgccgg tgacgctgcg caaatccgcg
    15661 tacagattga ggttctccat caccgtgaga tcttcataca gaccaaattt ctgcggcata
    15721 tagccgagca cggcgtgcag cgcgccgtcg tttttgatcg gatcaaagcc aatcaccgtg
    15781 gcactgccgc tgtcgggttt cagtaatccc gccaacatcc gcatcagtgt ggttttacct
    15841 gcaccgtccg gccccaccaa ccccgtcaca taaccggcgt gaatggtaca atcgagcggc
    15901 gcgacggcgg gcttgtccat gcccggaaag cgtttttcca ggccgttcag cgtgataacg
    15961 gcatcattca tgtcctgcct cgtcaccgaa ttgtaccgtc actggcattc cctggcgtaa
    16021 cgcatcatcg gcgtcggtca ccacaatacg caggcgatag acgaggtcgg tacgcagatc
    16081 cggtgtttcg acggttttcg gggtaaattc agcagtcggc gaaacgaaac caatctgccc
    16141 gtgatacggc ttgtccgggc gaccatcggt ataaagcagc actttgcgcc ccggctgggc
    16201 ctggtcaaga ttccgttcat caacataagc gcgcacccac accggacgcg ttagtgaaac
    16261 ggtaaacacc gtgccacctt cattgagaac cgtgcctggc tctaccgcgc gcgttaacag
    16321 cgtgccatca gacggtgcta tcaacgttga gtcctgtaaa ttcagctccg cctgcgccag
    16381 ttgcgcctgc gcctgttcga ggctggcttt cgcctgagcg atgtcctgtt cacggttacc
    16441 ggaacggtac tgacgcaatt tatcctgtgc tgatttcagc gttgcctgtg cctggtcgcg
    16501 cgaggagcgg gcattttcca ggtcatttgc cgaaatggtg cggcttttcc acagtccttg
    16561 ctggcggtta tagaagttct gcgcatagtc ataggcggct tgcgcctgtt tcaccgctgc
    16621 ggcggcctga gcgatttctt catcgcgata cccggcaagc atcagatcat actgcgcctg
    16681 tgccaccgag acgcccgctt tcgcctgcat cagggcaatc tcatacggct tgtgatccag
    16741 ttcgcccagc acctggcccg ctttgatagc atcaccttcg tccaccgcca gcgattcaac
    16801 gcgcccccca acacggaaac taagattcac cgtacgaata tccacgttgc catacagcgt
    16861 caggccgtta tcctggcggc tttgatacca ccagtagcct ccggcaacca cggcggcaag
    16921 taccactacc gccagtccga tcacgacagg ttttttcatc actacaaact cctttgcgat
    16981 aatccttgca aaatcaggtc gatatgacag gtcaccgtct ggttgatcag ttcggtcttt
    17041 tcttcatcga acgcggtcca gccggtacgt aacagaattg tttctttgcc aagacggaac
    17101 gccagaatct caccaatcaa cgcatgggta tgaaggatca tgcgggtgtc attagcgtcg
    17161 cagccggtcc aggcggcaat caggcgtgtc aggtggctgt gtagcggact aatcacctgt
    17221 tcgtgcacca ggtggtaggc tgccgtggga gagagctgct cacgggagat aaacttgctg
    17281 aggttgacgg tgtcatcctg ggtgagcagc ttaatcatgt tcctgcaggc gcgaaggatc
    17341 agttcacgga tggcagcccg atcaggctgt ggttgggcga acaagcgttc ggcttcctcg
    17401 gcatgcggac ggaactgctc gccaataaaa tcggcaatcc actgggcgca ggcgaggtac
    17461 aaatcttctt tcgaaccgaa gtagtaggtg atggcagcga tattctgccc ggcctgggcg
    17521 gctatctcgc gggtggtggc gttcattcca tattcaccaa actgcgccag tgcggcagca
    17581 atcagctgtt ttttcgcctg ttcacccttg attgtcatgg caggattatt catcgcacag
    17641 tccattctta atcaaatgat tgattaagat tatgactcca tatgggagtt gtccagtatg
    17701 gctaagaatt ttagctacgc cagtcacagg gataatttat gcgctgcgtc acaaaaactg
    17761 ctacactccg ctccctcatg acattgtggt ttttgtcatt ttccttttca gtatctccct
    17821 gaaaactaca ccggtaacgg tcggggcggt tcggagtagt tatgtctttc gattctttgg
    17881 gtttaagccc tgatatcctg cgcgccgttg ccgagcaggg ttaccgtgaa cccaccccta
    17941 ttcagcagca ggcgatccct gctgtgctgg aaggccgcga cctgatggct agcgcccaga
    18001 ccggcaccgg caaaacagcg ggctttacgc tgccgctgtt gcaacacctg atcactcgcc
    18061 agccgcacgc caaagggcgt cgtccggtac gtgcgctcat tcttaccccg acccgtgaac
    18121 tggcggcaca gattggcgaa aacgtccgtg attacagcaa atacctgaac attcgttcgc
    18181 tggtggtgtt tggcggtgtc agcattaacc cacaaatgat gaagttgcgc ggcggcgttg
    18241 atgtgctggt ggcaacgccg ggacgtttgc tggacctgga acatcaaaat gcagtgaagc
    18301 tggatcaggt tgaaatcctc gtcctcgatg aagctgaccg catgctcgac atgggcttta
    18361 tccacgatat ccgtcgcgtg ttaacaaaac tgccagcgaa acgccagaac ctgctgttct
    18421 ctgcgacctt ctctgacgat attaaagccc tggcggaaaa actgttgcac aacccgctgg
    18481 aaatcgaagt ggcacgccgc aataccgcgt ctgatcaggt gacccagcac gttcactttg
    18541 tcgataagaa acgcaaacgc gaattgctgt cgcacatgat tggtaaaggg aactggcagc
    18601 aggtgctggt gtttacccgt accaaacacg gcgctaacca tctggctgag cagctcaata
    18661 aagatggcat ccgtagtgtg gcgatccacg ggaataaatc gcaaggtgcg cgtacccgtg
    18721 cgctggctga ttttaaatcg ggtgacattc gcgtactggt ggcgaccgat atcgctgcgc
    18781 gcggtctgga tatcgaagag ttgccgcacg tcgtcaacta tgaactgcca aacgtaccgg
    18841 aagattatgt ccaccgtatc gggcgtaccg gtcgtgcggc tgctaccggt gaagcgttgt
    18901 cgctggtgtg tgttgatgaa cacaaactgc tgcgtgatat cgaaaaactg ctgaaaaaag
    18961 agatcccgcg cattgcgatt ccgggctatg agccggaccc gtcaatcaaa gccgaaccga
    19021 tccagaacgg tcgccagcaa cgtggcggca tcaggctgat gaacaaacgc aaaactgcct
    19081 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    19141 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    19201 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    19261 tctctatagc caattgttcg cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    19321 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    19381 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcgcgaagtg
    19441 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    19501 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    19561 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gtattcccgt
    19621 caaccttgat gggcctggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    19681 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    19741 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    19801 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    19861 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    19921 atccccgact ttattccccg tgcgccgcag cggcagatga ttgcggacgt cgccaaaacg
    19981 ctggccggag aagaagggcg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    20041 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    20101 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    20161 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtcca
    20221 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    20281 gacgacgaac tgacgccaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    20341 gatctcgaca cttataaatg ggatggtctg cgcgatcata cggatatcgc tatagatgat
    20401 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    20461 tatcgtgaat gcccgttttt tgttactcgt cgggagattc aggaagcgga agtggtggtg
    20521 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    20581 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    20641 gagatgagcg ctgaaatcac cgtgccctgg tatcgactac agctggactt gttcacgaaa
    20701 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    20761 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    20821 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    20881 cctgatgaat tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    20941 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccacga tattgtgcgt
    21001 ctgcatcggt tgattttaca gatgaaccgc gcgctgggga tgtttgaggt gcaaagcaaa
    21061 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    21121 cgggaagagc gcgaagggca gctacacctc tggtttcact gcgtgggaat acgtgtcagc
    21181 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    21241 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    21301 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    21361 attccccgga tgcgctttga gccttccatc gacaacgaag agcaacacat tgccgaaatg
    21421 gcggcctttt tccgtgaaca ggtggagagc aaaaaatatc tcggtatgtt ggtactgttt
    21481 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg cctgatgttg
    21541 ctggtgcagg gcgatcagcc gcgttatcgc ttagttgaac tgcaccgcaa acgcgttgcc
    21601 aacggtgagc gtagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    21661 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccg
    21721 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    21781 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    21841 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgacaaaaaa ttatggcaag
    21901 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    21961 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    22021 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    22081 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    22141 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggggaaggg
    22201 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    22261 cctgcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggct
    22321 aacctgacgc cattattggc gattctgctg cataaactcg gttttcctgt ggtggttcac
    22381 ggagttagcg aagatccaac tcgcgtgctg acagaaacca ttttcgaact gatgggtatt
    22441 acaccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    22501 atgcctgtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    22561 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgag
    22621 gcactgtgtc tttccagcgt ttcgcatccg gaatacattg gacgcgtcgc gaagttcttt
    22681 agcgatatcg gcgggtgggc gttactaatg catggtactg aaggcgaagt gtatgctaat
    22741 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    22801 aaacaggacc ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    22861 cagtggactg agcgttgcct ggctggcagc gaagcgattc ccgaatcgct gaaaatccag
    22921 atggcttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgatgg cctggcgcgc
    22981 gttaatcagg cattttaatt tttatctcag ccggatgagc cacgtcatcc ggcttttacc
    23041 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cgcgcccggc gtgcagcata
    23101 aaaaaacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    23161 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    23221 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattctc
    23281 atggtattgg catgatccca agctatgtgc gttcctggag tcaggggcat ctgcaaatta
    23341 accatcatgc caaaatcgtt aaagaggcgg gggcggcagt cacgctcgat ggcgatcgcg
    23401 cattcggtca ggtcgcggca cacgaagcga tggcgctggg gattgagaaa gcgcatcagc
    23461 acggtattgc tgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    23521 cggagcagtg ttcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    23581 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccc ttctgtgtgg
    23641 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    23701 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    23761 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttggggtcgc
    23821 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    23881 gggcgctttc tggcggtaaa acgacgcatc aggaaacgtt gcaaaccagc cccgatgcca
    23941 ttcttaactg catgaccact atcatcatca acccggaact gttcggcgcg ccggattgta
    24001 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccgcatgat gacgataagc
    24061 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag gagcagggga
    24121 ttccattgga tgcgggaagc tggcaggcca tttgtgatgc ggcgcggcag attggtatgt
    24181 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    24241 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    24301 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    24361 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    24421 atgcggcata aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    24481 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgccgct
    24541 ttctcagcca gtttcgcttc cagggcatcg aggggggatg cgccatcggc agaaaccacg
    24601 ccgattttat tcatgttctg tgcctgggat gccgttaccg gttccgccgc gaacacgcca
    24661 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    24721 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    24781 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    24841 tctaacatac tgataaacca tttatttgtt gtacttatgg acttgtttta tgccgcgtgg
    24901 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa tacaaattat actcgctatg
    24961 catctcgcca gttgtagtta acgtaactgt ctgtctttcg aacctctgcg attatatcct
    25021 gtatacgctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    25081 ggaatggctt cccgacgggc ttgcgggatg ggggcaccgc actcttcaca ttcatacagg
    25141 ctttcgccac gcggaatttc accccgagcg cgggcaatcg catcttcagt tgtactgttg
    25201 atctgttcgt tgacggcgtc atcgttagcc caagcggatg ccatatcgtc ctccccatat
    25261 caatgcttgt acagttaagt gtagctaata tagggacgaa cctgggcagt tcaagcgtca
    25321 gatctccgac cattcccgca gcagattatg ataaagattg agcagcgaca ggatctcttc
    25381 attttcacca tagcggcttt tcagcgactg aatatttttg tccagttcaa acagcatggc
    25441 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    25501 gcgggttacg ggtgtcacgc aatgcaggct gctggaggga tacaacacca gatcacctgc
    25561 cgggagtttt acccgatgtt gtccaaaggt gtcattaacg accagttcgc cgccgtcgta
    25621 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtgcgcatcc agccgttttg
    25681 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    25741 attaaacagc ggcgtggaaa gggtacgcgg caaggcagcg gcaaagaaca atgcatgttg
    25801 gttaaccgcg ttcagcacct cattttgcag tgcggcgtat aacgcgctgc gggtgtctac
    25861 ttgctgattg ttcttaactt gtgcaccttg tgctccggtg gtgacgcgtc catccaccca
    25921 ttcggcttgt tccagttgtt cgcgaaaatg ggcgacgtcc tgtggcgata acacgccggg
    25981 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    26041 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    26101 ctcttgttga ttgaggcgac gtaatcggta tcaaacaggt tgtaaacgtt tagctggaag
    26161 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttctgta
    26221 aatgctggcg ttcccaccgc gccgtctgaa cctttatgta tactgccgat atagcgtgcg
    26281 cccgcgccaa cagagatatc atcggttgcc tgatattggc tccataaggt gaaggcgtgc
    26341 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    26401 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attccctgcc
    26461 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt cccgtcatca
    26521 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacaggcgt
    26581 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    26641 tcggtgcggt tggcactgtt accactgcca gactgcgcaa gggcgaagtt gttgccgccc
    26701 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtggtac
    26761 agcgccccgg ctttccagtt caccagattg cccgacttgg cggtgtcgac ggtggtgact
    26821 ggagaacctt ttgccacacc agctgggcag gtgatggcac cgcgtccgct gccgccgcag
    26881 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    26941 aaatcacggg tgatttgcag cgtatcgaag gcgtaaattg cgaaggtatc cgtctgacca
    27001 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa ttctgctgtc aggatgataa
    27061 atatttaccg ccggtaacgt caccggatta acgccatagt tagtctgcgt ttcacgggta
    27121 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    27181 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgactag
    27241 gtccagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    27301 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    27361 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    27421 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgct
    27481 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    27541 ttgtgctgag tgacatgcag ataattaaga tacaaacgat ttgctgtacc aaggccaaaa
    27601 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgg
    27661 gttttttcgc ccatcacatt caggcgcacc gcggtagtat caccaatgac ctgattgacg
    27721 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    27781 tcattgcgcg gctgcttgct gatcatattg atcgagcctg tcggtgcgct gcgcccgtag
    27841 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    27901 acgctgccga tatcgcgaat accatcaata taaatactgt tagaggtatc ggcaccacgc
    27961 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    28021 c
//
LOCUS       DAZXUL010000052        27601 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZXUL010000052 DAZXUL010000000
VERSION     DAZXUL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.052"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACH3L1_002180"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACH3L1_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFT4076904.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACH3L1_002181"
     CDS             complement(1614..1937)
                     /locus_tag="ACH3L1_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFT4076905.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACH3L1_002182"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACH3L1_002182"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFT4076906.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACH3L1_002183"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACH3L1_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFT4076907.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACH3L1_002184"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACH3L1_002184"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFT4076908.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACH3L1_002185"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACH3L1_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFT4076909.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACH3L1_002186"
     CDS             7537..8574
                     /locus_tag="ACH3L1_002186"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFT4076910.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACH3L1_002187"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACH3L1_002187"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFT4076911.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACH3L1_002188"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACH3L1_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFT4076912.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACH3L1_002189"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACH3L1_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFT4076913.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="ACH3L1_002190"
     CDS             10745..11626
                     /locus_tag="ACH3L1_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFT4076914.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACH3L1_002191"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACH3L1_002191"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFT4076915.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="ACH3L1_002192"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="ACH3L1_002192"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFT4076916.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACH3L1_002193"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACH3L1_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="ACH3L1_002194"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="ACH3L1_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFT4076917.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="ACH3L1_002195"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="ACH3L1_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFT4076918.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="ACH3L1_002196"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="ACH3L1_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFT4076919.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="ACH3L1_002197"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="ACH3L1_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFT4076920.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="ACH3L1_002198"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="ACH3L1_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFT4076921.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="ACH3L1_002199"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="ACH3L1_002199"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFT4076922.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="ACH3L1_002200"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="ACH3L1_002200"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFT4076923.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="ACH3L1_002201"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="ACH3L1_002201"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFT4076924.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="ACH3L1_002202"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="ACH3L1_002202"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFT4076925.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="ACH3L1_002203"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="ACH3L1_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFT4076926.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACH3L1_002204"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACH3L1_002204"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFT4076927.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /gene="acpS"
                     /locus_tag="ACH3L1_002205"
     CDS             24893..25273
                     /gene="acpS"
                     /locus_tag="ACH3L1_002205"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFT4076928.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="ACH3L1_002206"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="ACH3L1_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFT4076929.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACH3L1_002207"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACH3L1_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFT4076930.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="ACH3L1_002208"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="ACH3L1_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6807 a         6865 c         7237 g         6692 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAZXUL010000053        27209 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZXUL010000053 DAZXUL010000000
VERSION     DAZXUL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27209
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.053"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..850
                     /locus_tag="ACH3L1_002209"
     CDS             <1..850
                     /locus_tag="ACH3L1_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFT4076931.1"
                     /translation="RHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGFN
                     ILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEI
                     VARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEI
                     YESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNP
                     LNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGT
                     IYPIEK"
     gene            872..2281
                     /locus_tag="ACH3L1_002210"
     CDS             872..2281
                     /locus_tag="ACH3L1_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4076932.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMTGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2259..3038
                     /locus_tag="ACH3L1_002211"
     CDS             2259..3038
                     /locus_tag="ACH3L1_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFT4076933.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3035..3895
                     /locus_tag="ACH3L1_002212"
     CDS             3035..3895
                     /locus_tag="ACH3L1_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFT4076934.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4043..4618)
                     /locus_tag="ACH3L1_002213"
                     /pseudo
     CDS             complement(4043..4618)
                     /locus_tag="ACH3L1_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4635..4895)
                     /locus_tag="ACH3L1_002214"
     CDS             complement(4635..4895)
                     /locus_tag="ACH3L1_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFT4076935.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4886..6157)
                     /locus_tag="ACH3L1_002215"
     CDS             complement(4886..6157)
                     /locus_tag="ACH3L1_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFT4076936.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6235..6597)
                     /gene="queD"
                     /locus_tag="ACH3L1_002216"
     CDS             complement(6235..6597)
                     /gene="queD"
                     /locus_tag="ACH3L1_002216"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFT4076937.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6916..8715
                     /gene="cysJ"
                     /locus_tag="ACH3L1_002217"
     CDS             6916..8715
                     /gene="cysJ"
                     /locus_tag="ACH3L1_002217"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFT4076938.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8715..10427
                     /gene="cysI"
                     /locus_tag="ACH3L1_002218"
     CDS             8715..10427
                     /gene="cysI"
                     /locus_tag="ACH3L1_002218"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFT4076939.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10501..11235
                     /gene="cysH"
                     /locus_tag="ACH3L1_002219"
     CDS             10501..11235
                     /gene="cysH"
                     /locus_tag="ACH3L1_002219"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFT4076940.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11499..11651
                     /locus_tag="ACH3L1_002220"
     CDS             11499..11651
                     /locus_tag="ACH3L1_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFT4076941.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11845..12936
                     /locus_tag="ACH3L1_002221"
                     /pseudo
     CDS             11845..12936
                     /locus_tag="ACH3L1_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13082..14119)
                     /gene="iap"
                     /locus_tag="ACH3L1_002222"
     CDS             complement(13082..14119)
                     /gene="iap"
                     /locus_tag="ACH3L1_002222"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFT4076942.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14371..15279
                     /gene="cysD"
                     /locus_tag="ACH3L1_002223"
     CDS             14371..15279
                     /gene="cysD"
                     /locus_tag="ACH3L1_002223"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFT4076943.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15281..16708
                     /gene="cysN"
                     /locus_tag="ACH3L1_002224"
     CDS             15281..16708
                     /gene="cysN"
                     /locus_tag="ACH3L1_002224"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFT4076944.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16708..17313
                     /gene="cysC"
                     /locus_tag="ACH3L1_002225"
     CDS             16708..17313
                     /gene="cysC"
                     /locus_tag="ACH3L1_002225"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFT4076945.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17363..17686
                     /locus_tag="ACH3L1_002226"
     CDS             17363..17686
                     /locus_tag="ACH3L1_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFT4076946.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17880..18191
                     /gene="ftsB"
                     /locus_tag="ACH3L1_002227"
     CDS             17880..18191
                     /gene="ftsB"
                     /locus_tag="ACH3L1_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFT4076947.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18210..18920
                     /gene="ispD"
                     /locus_tag="ACH3L1_002228"
     CDS             18210..18920
                     /gene="ispD"
                     /locus_tag="ACH3L1_002228"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFT4076948.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18920..19399
                     /gene="ispF"
                     /locus_tag="ACH3L1_002229"
     CDS             18920..19399
                     /gene="ispF"
                     /locus_tag="ACH3L1_002229"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFT4076949.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19396..20445
                     /gene="truD"
                     /locus_tag="ACH3L1_002230"
     CDS             19396..20445
                     /gene="truD"
                     /locus_tag="ACH3L1_002230"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFT4076950.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20426..21187
                     /gene="surE"
                     /locus_tag="ACH3L1_002231"
     CDS             20426..21187
                     /gene="surE"
                     /locus_tag="ACH3L1_002231"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFT4076951.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21181..21807
                     /gene="pcm"
                     /locus_tag="ACH3L1_002232"
     CDS             21181..21807
                     /gene="pcm"
                     /locus_tag="ACH3L1_002232"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFT4076952.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21992..23086
                     /gene="nlpD"
                     /locus_tag="ACH3L1_002233"
     CDS             21992..23086
                     /gene="nlpD"
                     /locus_tag="ACH3L1_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFT4076953.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23149..24141
                     /gene="rpoS"
                     /locus_tag="ACH3L1_002234"
     CDS             23149..24141
                     /gene="rpoS"
                     /locus_tag="ACH3L1_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFT4076954.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24261..24668)
                     /locus_tag="ACH3L1_002235"
     CDS             complement(24261..24668)
                     /locus_tag="ACH3L1_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFT4076955.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24815..25408
                     /locus_tag="ACH3L1_002236"
     CDS             24815..25408
                     /locus_tag="ACH3L1_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFT4076956.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25408..26355
                     /locus_tag="ACH3L1_002237"
                     /pseudo
     CDS             25408..26355
                     /locus_tag="ACH3L1_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26628..27191)
                     /locus_tag="ACH3L1_002238"
     CDS             complement(26628..27191)
                     /locus_tag="ACH3L1_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076957.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
BASE COUNT         6566 a         6782 c         7354 g         6507 t
ORIGIN      
        1 tcgtcacatc atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa
       61 aatctttaaa tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa
      121 aaccggcttc aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg
      181 tttgtatgac gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt
      241 gctgatcttt atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc
      301 ggaaatcgtt gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg
      361 gtttaacaac ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa
      421 aaccggcaac atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat
      481 ctacgaaagc gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat
      541 gctgggcggt cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga
      601 ctacaacgtt gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa
      661 caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat
      721 tggtaaacat cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga
      781 agggctgaaa aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat
      841 tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc
      901 aggcgagaag ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt
      961 gcgaatggat gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg
     1021 cgcacacctg accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac
     1081 gcccgccatg caactgacgc cgtttatgac gggaatgatc ggcggctcgg cgctccttgg
     1141 tttgtttctt ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat
     1201 cttcaccttc agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac
     1261 gccagagcat cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc
     1321 agtaggtcac accttgctgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg
     1381 cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt
     1441 tatttccgaa aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt
     1501 gttgattacg ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg
     1561 gcgttttgca gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg
     1621 cgatgaagtg gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg
     1681 gcggcgcacg gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat
     1741 ttatacctgg ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag
     1801 cctgatgctt aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct
     1861 gctggcacat cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt
     1921 aatggcctgt ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac
     1981 caccatttcg gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga
     2041 cattcgctcg ctgggagtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag
     2101 tactggcctg ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct
     2161 agcgacagtg ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc
     2221 cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta
     2281 aagccgaacc ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta
     2341 atagcggacc ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg
     2401 ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca
     2461 tgggggatga acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag
     2521 ctgtattgct ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata
     2581 ttgccgaatg gcagcgtcag aatccgctgg atctgataat taccggcagc caaagcagcg
     2641 aagggcagaa tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca
     2701 cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac
     2761 atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg
     2821 aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta
     2881 tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag
     2941 agcaacgacg cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc
     3001 tatggcagga ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa
     3061 tcaggagaat gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct
     3121 ggcgcactgg caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt
     3181 ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga
     3241 tgaactgtcg acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc
     3301 gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac
     3361 gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa
     3421 aaacgccacg ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga
     3481 ctggctggtt agcacagagg atctgaaaaa tgttagccgc gacccattag ctgaggccag
     3541 acgggtgctg gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc
     3601 agaaaagctg ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga
     3661 tgctgaaaaa gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt
     3721 tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc
     3781 gattaatcat gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga
     3841 ctggaaagtg gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc
     3901 tgccggatga tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt
     3961 aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat
     4021 tcaattggtt acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat
     4081 tcgtggtgga aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc
     4141 ataccagccc gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt
     4201 tgggcatgca gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat
     4261 caatgttgtg aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt
     4321 ccgctcttgc cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca
     4381 ccagtttcaa aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat
     4441 gaataaaagc atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga
     4501 tattgccgta caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg
     4561 cattatcttt aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa
     4621 attatcctta ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc
     4681 cactgttgta gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg
     4741 cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg
     4801 ttaatcaacc gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca
     4861 tcagcaacgc gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg
     4921 ccagatgcca caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa
     4981 caggtttatc accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg
     5041 cccgatacct gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat
     5101 cccatagcag gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc
     5161 ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat
     5221 ccatgccgcg cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca
     5281 gccagccgtt accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat
     5341 gcgcaccata ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt
     5401 taaagcgagt cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc
     5461 aaacaatccc taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt
     5521 catcacagat tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac
     5581 gttcttcaat agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt
     5641 cgccagcagg acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg
     5701 ccagcaccac ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc
     5761 cgttttcttc atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt
     5821 cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa tacattccag gattcaccgc
     5881 cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt
     5941 gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg
     6001 catgggtata taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga
     6061 gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac
     6121 cggcaccaat aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac
     6181 acaacaccct aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc
     6241 gccgcgatag atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg
     6301 cacaacgggt ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc
     6361 cagacctgga atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc
     6421 cgctttcagt tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat
     6481 ttccagtcgc accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac
     6541 atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat
     6601 aatttctcta caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt
     6661 atctaaaacg actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat
     6721 ttgtttttca ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt
     6781 agtcgatttg gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccgtta
     6841 accttacctt ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact
     6901 ggaacataac gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg
     6961 gagcaactgg cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg
     7021 gtttctggct atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca
     7081 gcgcccgccg cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg
     7141 cgccgggttg ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg
     7201 gtgaacgcgg gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg
     7261 acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg
     7321 ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat
     7381 agctcttatg aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg
     7441 gggggtgaac gcctgctcga ccgtgtcgat tctgatgttg aataccaggc tgctgccagc
     7501 gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg
     7561 caatccgtcg ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg
     7621 ccgctggtgg ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac
     7681 gttcgccata tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg
     7741 ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg
     7801 ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta
     7861 cagtggcact tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt
     7921 acccgcagtg aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc
     7981 gcgacgacgc tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg
     8041 ctaattaatc tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg
     8101 gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc
     8161 gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc
     8221 cgcgtattta tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccggtg
     8281 attatgattg gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc
     8341 gctgacgaag cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac
     8401 ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat
     8461 ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag
     8521 ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct
     8581 aatcgcatgg cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc
     8641 atggacaccg aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag
     8701 cgagatgtct actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga
     8761 cagacgccga gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt
     8821 taaacgacgg tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg
     8881 gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc
     8941 cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt
     9001 ggcaggcgat cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca
     9061 accgccagac gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga
     9121 tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac
     9181 tctgcacctc gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga
     9241 agatttctga acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg
     9301 aaaaagtcgc caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt
     9361 tcaaaaccac ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga
     9421 acttcgtggc gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg
     9481 ggctttctat cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct
     9541 atctgccgct agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact
     9601 ggggtaaccg aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg
     9661 ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc
     9721 cgtatgaatt tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact
     9781 ggcacctgac gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga
     9841 aaactggcct gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc
     9901 agaatctgat catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca
     9961 aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt
    10021 cattcccgac ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg
    10081 acaacatcga taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg
    10141 taacaggctg cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ctggtgggta
    10201 aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac
    10261 ggatgtataa agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag
    10321 ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg
    10381 gcatcattcg cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt
    10441 cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct
    10501 atgtccaaac tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg
    10561 ctggcagaaa ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg
    10621 ctggataatc tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg
    10681 agcctgcatc tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc
    10741 tatctgttcc cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac
    10801 ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg
    10861 tgggaacagg gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg
    10921 aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa
    10981 tccggcagcc gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg
    11041 ccgattatcg actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa
    11101 tatcacccat tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa
    11161 tgggaacccg gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga
    11221 ttacacgaag ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc
    11281 gtttttattt tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac
    11341 aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca
    11401 gatggacaaa agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca
    11461 attcggatgt tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg
    11521 tggcaatcat cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt
    11581 tatgtgagtt gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat
    11641 cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc
    11701 tcctgcaccc tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat
    11761 tatgaatttc aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata
    11821 tatatcatag atagttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa
    11881 aatattgtga ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc
    11941 gacgactatc accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg
    12001 gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag
    12061 gaacaggcag ctcgattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc
    12121 cgctcttttc aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa
    12181 aattacgaaa aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt
    12241 gaatgtcagg agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata
    12301 gaaatgtgga tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa
    12361 ttggataatt ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag
    12421 ccactgtttc cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt
    12481 ataaaacaac tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg
    12541 tcaacgcgat tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag
    12601 gctttagttc aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct
    12661 ttcaccggaa ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag
    12721 gtgctggatc tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc
    12781 ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac
    12841 gctcaggggc tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga
    12901 ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata
    12961 aaccggcgca atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat
    13021 gggtatcact tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc
    13081 atcaagacgc cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc
    13141 agcgacgttc tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta
    13201 cgtcatgcca gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat
    13261 ccttatttcc aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt
    13321 cgaatatttc tgcgtcatta caacccccag tgcctttcgg ataattttta ttcaaacccg
    13381 gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta
    13441 atttccttac tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa
    13501 ttaagttatc gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct
    13561 tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa
    13621 taccatactc ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac
    13681 ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag
    13741 catcgctcag cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt
    13801 tgccttcatg agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat
    13861 cgcgggcggt ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt
    13921 gctgaaactg ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc
    13981 gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag
    14041 gtgacgaggc gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt
    14101 ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata
    14161 gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt
    14221 gccttaagca ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca
    14281 ttctaattgg taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca
    14341 agaaaatagc ggtattgcaa aggaacggtt atggctcaaa aacgacttac tcacctgcgg
    14401 caactggagg cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg
    14461 gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg
    14521 ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc
    14581 gagatgtatg agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat
    14641 aaaaacccgg aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat
    14701 accgatatta tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc
    14761 gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct
    14821 ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac
    14881 tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc
    14941 gagcaggata tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc
    15001 gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt
    15061 atcgacctgc aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc
    15121 tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa
    15181 gagatgctgg tttccaccac cagtgaacgt cagggtcgcg tgattgaccg cgaccaggcg
    15241 ggatccatgg agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca
    15301 acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct
    15361 gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct
    15421 gctgcacgat acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa
    15481 gcgtcacgga acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc
    15541 tgagcgcgaa cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg
    15601 taaatttatt atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg
    15661 cgcatcgaca tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca
    15721 aacccgtcgt cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat
    15781 caacaaaatg gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta
    15841 tctgaccttt gccgggcagc tgccgggtaa tctggatatc cgctttgtgc cgctctccgc
    15901 actggaaggt gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac
    15961 actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg
    16021 cttcccggtg cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct
    16081 ggcatccggt cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc
    16141 aaacgtcgcg cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc
    16201 gatcaccctg gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc
    16261 agacgaagcg ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca
    16321 gccgctttcc ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg
    16381 tgttgatggc attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa
    16441 cctgccgctg aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga
    16501 tcgctatcag caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt
    16561 gaccgtgggt gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga
    16621 attcagtgca ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg
    16681 cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat
    16741 agtcatccgg tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg
    16801 tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg
    16861 ttacataaac tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta
    16921 tgcagcgatc tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa
    16981 gtggcgaatt tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac
    17041 cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt
    17101 gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg
    17161 cgtgccggtg aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg
    17221 gcagaaattc atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat
    17281 ctgttgagac agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt
    17341 cgtcaaagta acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga
    17401 cagccttact gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat
    17461 ctcctggtta tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt
    17521 ctttctctac acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat
    17581 tgcgctgcat tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac
    17641 agtaggcatt atgtttggcg cactgtttat gtggctactg ggctaatttg tactttcttg
    17701 tctcctctgt tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg
    17761 gtatacgtaa caatgggcaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca
    17821 gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga
    17881 tgggtaaact aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt
    17941 tcggtaagaa cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag
    18001 ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca
    18061 atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg
    18121 gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa
    18181 acaatcgata aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg
    18241 tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct
    18301 caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg
    18361 tgacacgtgt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg
    18421 cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg
    18481 caggtctgaa agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt
    18541 gtttgcatca ggatgacctt gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag
    18601 ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca
    18661 ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc
    18721 gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg
    18781 aagcctcggc gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata
    18841 acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca
    18901 tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg
    18961 tgaaggccca attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca
    19021 ttctgatggc gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct
    19081 gggggatatc ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg
    19141 cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt
    19201 cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt
    19261 gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac
    19321 ggaaaaactt ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact
    19381 cattaaggca acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa
    19441 ggcaccgggc tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt
    19501 gagcctgatg gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc
    19561 cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc
    19621 gctgggcaaa aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc
    19681 aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg
    19741 cggcacaagc gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg
    19801 cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt
    19861 gtaccgaact acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg
    19921 ctgcgctggg cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg
    19981 tcggcagccc gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac
    20041 gttaatcaag ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta
    20101 gcaaccaccg aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata
    20161 accgccgcat tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa
    20221 caagcagctg tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa
    20281 gccgcgcgca gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac
    20341 gtcaccgtag agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg
    20401 gaacttatca acacaacagg tgattatgcg catattgctg agtaattatg acggggtaca
    20461 tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt
    20521 cgcccccgat cgtaaccgca gcggcgcttc aaattctctg acactggaat cctctctgcg
    20581 cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt
    20641 ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa
    20701 cgccgggccg aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga
    20761 aggccgtcat ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga
    20821 cactgccgcg gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac
    20881 cggacgtatt cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg
    20941 cgtgacgcgc tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg
    21001 cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac
    21061 cgattttgct gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac
    21121 tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca
    21181 atggtaagca gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat
    21241 gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt
    21301 gaacaaaaag cctgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag
    21361 ccgtatatgg tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg
    21421 gaaattggca ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt
    21481 tgctcggttg aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt
    21541 gatttacata atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg
    21601 ccgtttgacg ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg
    21661 cagctggacg aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa
    21721 cgggtgcgtc gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc
    21781 cctttagtga agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta
    21841 ttttgccgta ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca
    21901 cggtaattat gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag
    21961 cccaaaattc accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg
    22021 ctgttctgac acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc
    22081 aaatactaat tctggtatgt tgattacgcc gccgccgaaa atggggacga cgtctacagc
    22141 gcagcaaccg caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca
    22201 aatccagcca gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt
    22261 ctataaccgt cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt
    22321 gaaaaaaggc gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct
    22381 tgctcagcgc aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt
    22441 gggtaatgct tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc
    22501 agagcaagga gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc
    22561 gacaattacg tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa
    22621 caagccaact gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga
    22681 gccgactgtc agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga
    22741 gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc
    22801 aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa
    22861 cgcgctgcgc ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc
    22921 ctacgcccat aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa
    22981 aatagcaacc atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta
    23041 caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag
    23101 gcttttgctt gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat
    23161 acgctgaaag ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt
    23221 tttgacgaaa aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa
    23281 ctgttatcgc agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag
    23341 attggttatt caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg
    23401 cgtggagatg tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa
    23461 attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac
    23521 ttggggctga tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca
    23581 tacgcaacct ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact
    23641 attcgtttgc cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag
    23701 ttgtcccata agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag
    23761 ccagttgatg acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc
    23821 ccgctgggtg gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac
    23881 ggtccggaag ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc
    23941 gagctgaacg ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa
    24001 gcggcaacac tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag
    24061 attcaggttg aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc
    24121 gaagcgctgt tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg
    24181 gcctttttct tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt
    24241 cctacccggc aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc
    24301 tcctgctctt ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt
    24361 atcgccgcag caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg
    24421 tcagcgacgt catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct
    24481 gctagcgttg ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt
    24541 ttatcagcaa tggcgcgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc
    24601 tgccagcgag ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt
    24661 aacgccatgt aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga
    24721 agaagatgtt gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat
    24781 aacgttcgta tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg
    24841 ggctaccggt gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt
    24901 cgagactcat ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta
    24961 cagcgctcgc gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc
    25021 gatcatctca tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa
    25081 aacgctcgcc ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt
    25141 cgtgctcaaa gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat
    25201 ccatctcgaa aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc
    25261 tgccttttat aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt
    25321 gctggatcaa tttggcctcg aacatcccca cgccaggcgc tggcaaggat tgccgcaggc
    25381 ccggaatttt tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag
    25441 gcgcttgatg accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat
    25501 ctggcagcag cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt
    25561 gataatattc gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag
    25621 aaccacgcga tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag
    25681 tttatccgcc gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg
    25741 gcgcagaaca ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc
    25801 ctgaatgatg gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg
    25861 ctcgacccgg ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc
    25921 aagcgtaagc tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa
    25981 gcagaagagc gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc
    26041 acgctgatgg gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg
    26101 ctgcgtgaaa gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg
    26161 ggttcagaag tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg
    26221 ttcggtgagt ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat
    26281 aaagtctctt atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc
    26341 gaaatcgact acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag
    26401 acgggaatag attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc
    26461 aaaaatgtta aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa
    26521 tagcctttaa agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa
    26581 gcgccaaaac caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct
    26641 gctttctgag gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta
    26701 accttttcgt ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga
    26761 gcgtgcaact cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc
    26821 ccaagaacat cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga
    26881 atttgagcat caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca
    26941 acatcaccct ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt
    27001 tttaactgat acgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa
    27061 gtgccttcat tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact
    27121 aacctaaaga ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt
    27181 gcttttctca ttcttggttc ctttctatc
//
LOCUS       DAZXUL010000054        26852 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZXUL010000054 DAZXUL010000000
VERSION     DAZXUL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26852
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.054"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            24..563
                     /locus_tag="ACH3L1_002239"
     CDS             24..563
                     /locus_tag="ACH3L1_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFT4076958.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            861..1745
                     /gene="tsx"
                     /locus_tag="ACH3L1_002240"
     CDS             861..1745
                     /gene="tsx"
                     /locus_tag="ACH3L1_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFT4076959.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(1922..2269)
                     /gene="yajD"
                     /locus_tag="ACH3L1_002241"
     CDS             complement(1922..2269)
                     /gene="yajD"
                     /locus_tag="ACH3L1_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFT4076960.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(2398..3369)
                     /gene="secF"
                     /locus_tag="ACH3L1_002242"
     CDS             complement(2398..3369)
                     /gene="secF"
                     /locus_tag="ACH3L1_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFT4076961.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(3380..5227)
                     /gene="secD"
                     /locus_tag="ACH3L1_002243"
     CDS             complement(3380..5227)
                     /gene="secD"
                     /locus_tag="ACH3L1_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFT4076962.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(5255..5587)
                     /gene="yajC"
                     /locus_tag="ACH3L1_002244"
     CDS             complement(5255..5587)
                     /gene="yajC"
                     /locus_tag="ACH3L1_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFT4076963.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(5610..6737)
                     /gene="tgt"
                     /locus_tag="ACH3L1_002245"
     CDS             complement(5610..6737)
                     /gene="tgt"
                     /locus_tag="ACH3L1_002245"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFT4076964.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(6792..7862)
                     /gene="queA"
                     /locus_tag="ACH3L1_002246"
     CDS             complement(6792..7862)
                     /gene="queA"
                     /locus_tag="ACH3L1_002246"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFT4076965.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            7955..8528
                     /gene="acpH"
                     /locus_tag="ACH3L1_002247"
                     /pseudo
     CDS             7955..8528
                     /gene="acpH"
                     /locus_tag="ACH3L1_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(8533..10350)
                     /gene="malZ"
                     /locus_tag="ACH3L1_002248"
     CDS             complement(8533..10350)
                     /gene="malZ"
                     /locus_tag="ACH3L1_002248"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFT4076966.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(10506..11879)
                     /gene="proY"
                     /locus_tag="ACH3L1_002249"
     CDS             complement(10506..11879)
                     /gene="proY"
                     /locus_tag="ACH3L1_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFT4076967.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(11955..13274)
                     /gene="brnQ"
                     /locus_tag="ACH3L1_002250"
     CDS             complement(11955..13274)
                     /gene="brnQ"
                     /locus_tag="ACH3L1_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFT4076968.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(13681..14976)
                     /gene="phoR"
                     /locus_tag="ACH3L1_002251"
     CDS             complement(13681..14976)
                     /gene="phoR"
                     /locus_tag="ACH3L1_002251"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFT4076969.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(15034..15723)
                     /gene="phoB"
                     /locus_tag="ACH3L1_002252"
     CDS             complement(15034..15723)
                     /gene="phoB"
                     /locus_tag="ACH3L1_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFT4076970.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            15913..17115
                     /gene="sbcD"
                     /locus_tag="ACH3L1_002253"
     CDS             15913..17115
                     /gene="sbcD"
                     /locus_tag="ACH3L1_002253"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFT4076971.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            17112..20255
                     /gene="sbcC"
                     /locus_tag="ACH3L1_002254"
     CDS             17112..20255
                     /gene="sbcC"
                     /locus_tag="ACH3L1_002254"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFT4076972.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            20381..21562
                     /gene="araJ"
                     /locus_tag="ACH3L1_002255"
     CDS             20381..21562
                     /gene="araJ"
                     /locus_tag="ACH3L1_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFT4076973.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(21705..22613)
                     /gene="mak"
                     /locus_tag="ACH3L1_002256"
     CDS             complement(21705..22613)
                     /gene="mak"
                     /locus_tag="ACH3L1_002256"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFT4076974.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            22738..23649
                     /gene="rdgC"
                     /locus_tag="ACH3L1_002257"
     CDS             22738..23649
                     /gene="rdgC"
                     /locus_tag="ACH3L1_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFT4076975.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(23727..24182)
                     /locus_tag="ACH3L1_002258"
     CDS             complement(23727..24182)
                     /locus_tag="ACH3L1_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076976.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(24179..24514)
                     /locus_tag="ACH3L1_002259"
                     /pseudo
     CDS             complement(24179..24514)
                     /locus_tag="ACH3L1_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(24669..24953)
                     /gene="ppnP"
                     /locus_tag="ACH3L1_002260"
     CDS             complement(24669..24953)
                     /gene="ppnP"
                     /locus_tag="ACH3L1_002260"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFT4076977.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(25025..25702)
                     /locus_tag="ACH3L1_002261"
     CDS             complement(25025..25702)
                     /locus_tag="ACH3L1_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFT4076978.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(25960..26151)
                     /gene="yaiA"
                     /locus_tag="ACH3L1_002262"
     CDS             complement(25960..26151)
                     /gene="yaiA"
                     /locus_tag="ACH3L1_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFT4076979.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(26201..26725)
                     /gene="aroL"
                     /locus_tag="ACH3L1_002263"
     CDS             complement(26201..26725)
                     /gene="aroL"
                     /locus_tag="ACH3L1_002263"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFT4076980.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
BASE COUNT         6808 a         7178 c         6618 g         6248 t
ORIGIN      
        1 ttgcgacgag aaagggacga cctatgaata ctaacgtttt tcgactgctc ctgctgggaa
       61 gcctgttcag ccttagcgcc tgtgtgcagc aaagtgaagt gcgacagatg aaacacagcg
      121 tcagcacgct gaaccaggag atgacgcaac tcaatcaaga aacagtcaaa atcactcagc
      181 aaaacaggct gaatgcaaaa tccagcagtg gggtttacct tctgcctgga gcgaaaacac
      241 cggcaagact ggaaagccag atcggtactt tacgtatgtc gctggtgaat attacgcctg
      301 atacagatgg caccaccctt acactacgta ttcagggtga gtcgaatgat cccttgcccg
      361 cattcagcgg aactgttgaa tatgggcaaa ttcagggaac aatagacaac tttcaggaaa
      421 tcaatgtgca gaatcaattg attaatgccc ctgccagcgt cctcgccccc agcgatgttg
      481 atattccgtt acaattaaag gggatttctg tggatcagtt aggctttgta cgtatccacg
      541 acattcaacc tgttatgcag taaacgtatt tcgggacgat tttgtgcgtc ccgcaacatc
      601 ttccccgtca ttttgttact ctgcttacat cacctggatt gatagtaaaa gtttgcaaca
      661 agggcgaaag tcagtacaat ccccgcccga atgtgtgtaa acgtgaacgc aatcgattac
      721 gtaaatgata gaactgtgaa acgaaacata tttttgtgag caatgatttt tataataggc
      781 tcctctgtat acgaaatatt tagaaacgca atttgcgcct ttttcactcc cgcaagggat
      841 tttcaaacag tggcatacat atgaaaaaaa cattactggc agccggtgcg gtactggcgc
      901 tctcttcgtc ttttactgtc aacgcagctg aaaacgacaa accgcagtat ctttccgact
      961 ggtggcacca gagcgttaac gttgtcggaa gctatcacac ccgtttcgga ccgcagatcc
     1021 gcaacgatac ctaccttgag tacgaagcat tcgctaaaaa agactggttc gacttctatg
     1081 gttatgcgga tgcgccggta ttcttcggcg gtaactccga tgctaaaggt atctggaacc
     1141 acggttctcc gctgtttatg gaaatcgaac cacgtttctc catcgacaag ctgaccaata
     1201 ctgaccttag cttcggtccg ttcaaagagt ggtacttcgc gaacaactac atttacgaca
     1261 tgggtcgtaa taaagatggt cgccagagca cctggtacat gggtctgggt accgatatcg
     1321 acactggcct gccgatgagc ctgtccatga acgtctatgc gaaataccag tggcagaact
     1381 atggcgcagc gaacgaaaac gagtgggacg gttaccgttt caaaattaaa tactttgtgc
     1441 cgattaccga tctgtggggc ggtcagctga gctacatcgg cttcaccaac ttcgactggg
     1501 gttccgattt aggggatgac agcggtaacg caatcaacgg tattaagacc cgtactaata
     1561 actctatcgc ttccagccat attctggctc tgaactacga tcactggcac tactctgtcg
     1621 tagctcgtta ctggcacgac ggtggtcagt ggaacgacga tgcagaactg aacttcggca
     1681 acggcaactt caacgttcgc tctaccggct ggggtggtta cctggtagta ggttacaact
     1741 tctgattatg aaaatgccgg gatttattcc cggcatttct gattgttaac aacgtgtgct
     1801 ttgttcatgc cggatgcggc gtaaacgctt tgtccggccc acaaaagcac gtaaattcaa
     1861 tatattgctg aaataacgta ggcctgataa gcgtagcgca tcaggcaatt ttacgtttta
     1921 atcacttctt cttgttcatc atcgctttca ggtcagcgaa tgggttgtac ttcgcttcac
     1981 cgacatcttt ctgcgcatct tcccctgcga taacggtcgt accatactga tccgcttcgg
     2041 tatatttcga atgctcatga tcgtggcaat agagacacaa caattcccag ttactgccat
     2101 cttccgggtt attggtatgg tcgtgatcaa tgtggtgaac ggtaagttca cgcaggttag
     2161 aataaacaaa ctcgcgggaa cagcgaccgc agacccacgg atagattttt aatgcttttt
     2221 cgcgatagcc gctttctaac cgtgcatagt tttttgggat gatagccatg aaaagttacc
     2281 tggatgaaat gtagggttac gcgcagtttc ccaagatgcg gggattttcg caatctccat
     2341 acatcaaaaa aaatcccgat cttctgaccg ggattttcaa catcaacggg aacttgatta
     2401 aggcagaatt gacggctgat ccgccccttc tttttccact ttctgctgca acatgtgttc
     2461 gcgcttcata cccagtttca gagccaacgc agatgctacg tagatggaag atgcagtgcc
     2521 gatggaaaca ccgataagca tggtcagcga gaagccttcc agtaccggac caccgaagag
     2581 atacagcatc aggataacca tcaaggtagt accggatgtg atcaaggtac ggtgcagcgt
     2641 ctgggtcaag gacacgttaa agatttcgta aggcgtaccg cgacggatct tgcggaagtt
     2701 ttcacgaatt cggtccgata ccacgatact gtcgttaagc gagtaaccga taaccgacat
     2761 caacgatgcc acaatggtca ggtcaatctc gatatggaat aacgacaaaa tacccagcgt
     2821 aatgataacg tcgtgcgtca gcgcaataac cacccctgcc gccagtcgcc actcaaagcg
     2881 gaaacctacg tacacgagga tagacagcag cgctgccatc aacgccatcg caccggtttg
     2941 cgcaaggtca gcccccacgc tcggaccgac gaactcaata cgcttcactg ctgcattctg
     3001 attggtggat tcgttaatca ccttcagaac ttggctgccc agcacctgac cgccggtttc
     3061 gccttcagca ggcggcatac ggaccatgat gtcgtggctg ctaccaaagt tttgcagcat
     3121 tggctcttca aaaccggctt tttgcaatgc atcacgcatt acgtcaattt cagccggttt
     3181 ttcgagcgta atttcgataa ccgtaccacc ggtgaaatcc agcccccagt taaagccgcg
     3241 cacgcccata ataacgatag cagcgattaa cagcagacca gagatgccga aagcccagta
     3301 gtcccagcgc ataaagtcat agactttacg gccgtggttt agttgttcaa cagtatattc
     3361 ctgtgccaca tcgcactcct cagattgaca gcttcttgac gcgcttgccg ccatatagca
     3421 ggtttacgat ggcacgcgta ccgacaatcg cggtaaacat cgacgtcgcc acaccgatac
     3481 cggtagtaat cgcgaaccct ttaattgccc cggtacccac tgcgtacagg atgatgactt
     3541 taatcagcgt ggtgatgttc gcatcgaaga tagaactgaa tgcgccacga taaccttcat
     3601 caattgcctg ttgaacagta cgtccgttgc tcaactcttc tttaatacgt tcgttgatca
     3661 gtacgttcgc atcgaccgct accgcaaggg ttaagacgat acccgcgata cctggcatac
     3721 tcagtgttgc gcctggcaac agcgacataa taccgacgat caagatcaag ttggcaatca
     3781 gagcactggt cgcaatcaga ccaaacttct tatagaagat gatcatgaac agaatagaaa
     3841 ccagcagacc ggcaaggcaa gcctccagcc cctgctcaat gttctgcata cccagggtcg
     3901 ggccaattgt acgttcttca acaatctgaa tcggcgcgat caacgcaccg gcacgcagca
     3961 gcagtgacag ctgacgggct tcgttcgggt tgttgatgcc ggtgatacgg aagctgttac
     4021 ccagacgaga ctggatgttg gcgatgttaa tcacctcttc ctgtttcacc agaaccgcac
     4081 gaccatttgc atctttctta ccgctgtctt tgtactccac aaacagggtt gccatcggtt
     4141 tgccgatgtt gtccttggtg aagttagaca taatgttgcc accagcgcta tcgagcgaga
     4201 tgttaacctg cggctggttg tattcgtcct ggctggaagt ggagtcggtg atatggtcac
     4261 cagtcagaat tacgcgtttg tacagcacaa ccggctgacc ttcgcgggtc tgtttcactt
     4321 cagagtcgcc cggtacgcga ccggatgccg cagcggcctg atcaacattg gtgtttacca
     4381 gacggaattc cagcgttgcc gtcgcgccca gaatctcttt cgcacgcgca gtgtcctgaa
     4441 taccgggcag ttcaacaacg atacggtcag caccctgacg ctgaaccacc ggctccgcca
     4501 cgccaagttg gtttacacgg ttacgcagga tattaatgtt ctgctgcacc gcatattcac
     4561 gcgcttcact cagacgagca tcgctcatta ccgcacgcag ctggttgctg ccctggctgc
     4621 taatcaccag gtccggatgg cgcttgctca gatacgcaat ggcttcatca cgagctttag
     4681 catcgcggaa agtgatgctc aggccgtagt tgttttcttt acgaacagtg gtatacggga
     4741 tgcccttttc gcgcaggtca ctgcgtaggc tatcgatatt ttgttcctgg agtttgccaa
     4801 gcgcggtgtc catatccact tccatcagga agtgaacgcc gccacgcagg tcaaggccga
     4861 gcttcatcgg ctcagcatga atagctgcca gccagcgcgg cgttgccggg gcaaggttaa
     4921 gcgccacgac gtatttgtca cccataacgc ccattaatgc ttcacgagcg cgcaactggg
     4981 tgtcagtgga gtcgaagcgc gcaagaatag cgccctcttc cagtgccaca gacttagcag
     5041 ttattttttc ttcttgtaac gttttctgga cctggatcag cgtttgctca ctggcggcga
     5101 ctccgcgcgc accagtgatc tgaacagccg gatcctcacc aaacaggttg ggaagcgcat
     5161 acagcagacc gatgacaatc accacgatca gcatgacgta cttccacaaa ggataacggt
     5221 ttaacacggc aattccctta gggaaaaatt ttaattacag cgccttcatg gtgcctttcg
     5281 gcaggacggc agctacgaag tcacgtttaa taactacttc agtggtgtca ttcagcgcga
     5341 tagcaatgta gccgttttcc gctactttgg ttacgcgacc aaccaggcca ccgttcgtca
     5401 gaacttcatc acctttggca atggagtcca tcagcttttt gtgttctttg gtgcgcttct
     5461 gctgtggacg caggatcatg aaatagaaaa tcagaccgaa taccaccagc atcaaaatca
     5521 aagacatcgg gctaccttgc gccggtgcac ccgttgccgc taccgcatca gaaataaaaa
     5581 agctcattaa atttccctca ttattaatat taatcaacgt tcaaaggtgg tacttctcgc
     5641 ccctgacgct ggtaaaaatc agttacgaag ctctctaatt taccctcttc aatagccttg
     5701 cgtaaacccg ccatcaaacg ctggtagtaa cgaaggttat gaatggtgtt gagtcgcgcg
     5761 cctaatattt cgttgcaacg gtcgagatga tgcaagtaag cgcgtgaata attgcgacag
     5821 gtgtagcaat cacactcagg atcgagtggg ccagtatcgc tcttatattt cgcattgcgg
     5881 attttcacca cgccatcggt cacgaacaaa tgaccatttc gggcgttgcg ggttggcatt
     5941 acgcagtcaa acatatcgat accgcgacgt acgccttcaa ccaggtcttc cggtttacca
     6001 acgcccatca ggtaacgcgg tttgtctgcc ggaatttgcg gacacacatg ctccagaata
     6061 cggtgcatat ctgctttcgg ctcacccaca gccagaccgc cgacagcgta gccatcaaag
     6121 ccgatatcta ccagaccttt aacagatata tcacgtaaat cttcgtaaat actgccctga
     6181 attataccaa ataacgcatt tttgttacca agactgtcaa aacgctcacg gctacgcttc
     6241 gcccaacgca gagacatctc catagagcgt tttgcgtaat cccagtcagc aggatacggc
     6301 gtacactcat caaagatcat gacgatatcc gaaccaagat cgtactgaat ctccatcgac
     6361 ttttccgggt cgaggaaaat cgggtcgccg ttgatcgggt tacggaagtg aacgccctgt
     6421 tcggtgattt tacgaatatc accaaggctg aagacctgga agccgccgga gtcggtaaga
     6481 atcggtcctt tccactgcat aaaatcgtgc agatcgccgt gcagtttcat gatttcctgg
     6541 cccgggcgca gccacaggtg gaaggtgttg ccgaggataa tttgcgcgcc agtggcttca
     6601 acttcttccg gcgtcatccc ttttacggtg ccgtaggtgc caacaggcat aaaacaaggc
     6661 gtttccacta cgccacgatc aaagaccagg cggccacggc gtgcgcgacc gtcggtggtg
     6721 tccagttcaa atttcatttt ttctccacta cgtcagaaaa acagtccaac gtttaaacca
     6781 gcgccgcgga attactcccc gacgcgctca ttaattgcct gcggattgta cgtgataaac
     6841 atcgcatcac cgtaactaaa aaagcgatat ttctcttcta ccgccgcttt ataggcattc
     6901 atggtgtgtt gataaccggc aaaggccgaa accagcataa tcagcgtcga ctctggcaag
     6961 tggaagttcg tcaccagcgc atcgaccact ttgtactgga agcccggata gataaagatt
     7021 tgggtatcgt caaagaacgg ttcgataaga tcgttttttg ccgcctgtgc cgcgctttcc
     7081 agcgaacgta ctgaagtggt gccaaccgca atcacccggt taccgcgcgc tttcgccgcc
     7141 agtaccgcgt ctaccacatc ctgcggtact tcagcgtatt ccgagtgcat gatgtgatct
     7201 tcaatggtgt cgacgcgcac cggctggaag gtgcccgcac caacgtgcaa cgtcacaaac
     7261 gccatctcca cgcctttggc gcgtaatttt tccagcaaag gctcgtcaaa atgcagacca
     7321 gcggtcgggg ctgcaaccgc gcccggtttt tcgctataaa cggtttgata aagttcgcgg
     7381 tcagcgtctt cgtccggacg atcgatatac ggcggcagcg gcatatggcc gatgctgttg
     7441 aggatatcca gtaccgagcg ttcatcatta aattcgactt caaacagtgc gccgtggcgc
     7501 gcggtcattg ttgcgttgat actttcgtca tcgcccagca acagttctgc gccaggtttt
     7561 ggcgctttcg aggcgcgaat atgcgcaaga atgcgtttgt cgtcgagcat ccgctcaacc
     7621 agcacttcaa tcttgccgcc gctggcttta cgcccaaaca gacgcgccgg gatcacgcgg
     7681 gtattattaa aaaccagaag atcgccgggg ttgagcttat cgagtaaatc ggtgaaagta
     7741 ccgtgcgtca gcgcgcccgt cggcccgtcc agcgacagta aacgacagct actgcgttca
     7801 ggcatgggat agtgggcaat cagggattcg ggcaattcaa aggagaaatc ggtaacgcgc
     7861 atgacactga ctcagactaa aataagagtc gggtagtcta gtgccggggc gctctccctg
     7921 caacctttac ccccctttgg atagaatata atcgatgaat tttttagctc acctgcattt
     7981 agcccatctc gcggaaagct cgctttctgg caatttactg gctgatttcg tacgcggaaa
     8041 tcctgaagaa agttttccgc ccgacgtcgt ggctggcatt catatgcatc gacgtattga
     8101 ctgacaatct gccggaagtc cgcgaagcac aggagtggtt tcgtagtgaa acgcgccgcg
     8161 ttgcgcctat tacgctggat gtcatgtggg atcactttct ttcccgccac tggtcgcagc
     8221 tgtcgccgga ctttccgcta caggaatttg tctgttatgc ccgcgagcaa gtgatgacga
     8281 ttttgccgga ctcaccgcca cgttttatca atctgaacaa ttacttgtgg tcagagcagt
     8341 ggctggtgcg ctatcgcgat atggatttca tccagaacgt gttaaacggc atggcaagcc
     8401 gccgcccacg tctggatgcc ctgcgtgact cctggtacga tttagacgct cattatgacg
     8461 ccctcgaaac ccgcttctgg cagttttatc cgcggatgat ggcgcaggcg tcacacaagg
     8521 cgttataaaa cgttagttca tccataccgt agccgaaatg gcaggcaaag ccagaatccc
     8581 gtcagtcagt tgcccatgcc cttctttgca ttgccattgc acggcattga gaaacggtga
     8641 cgcgggtagc accacttcac tggcctcgcc acggttgatt gccaccagta cacgttgctg
     8701 attcagcacg cggacaaata ccactacgtt atcttccgca tacagcacct gacagccgcc
     8761 acgacgcagc gcctgacttt tcttacgcag cacaatcatt cgccggtaca gcgcgaataa
     8821 cgccgtatcc tgcttttcca cctgccaggg gaacggttta cggcaaaacg gatcgttttt
     8881 accatccagt cccacttcat caccgtaata aatgcacggt acaccaggcc aggtgaacag
     8941 ccagaccacc gccagcggca ggcgcgcaat atcccgaccg agcagcgttt taaatcgcgc
     9001 agtatcgtgg ctatcgagct gattaaacat acgtaattgt tgttgatgag aaagccctgc
     9061 gcggtaatta tccatccagg ccatacaggt ttgggcgtca atttgctgcg gatcataaga
     9121 gatgtcggta ttggcaagaa atccccacaa cgggaatgtg aagccacgat agttcatggc
     9181 agcatcttcc acatcggcct gtaaccattg ccgtgcatcg ccaaaatgtt cgccgacaat
     9241 atacgcttcc ggctgggttt ctttcgccgc ttcggtgatc ccggcaacgt gctgcatatt
     9301 attgcgcgcc ccacccgcct cccccagcat atgcaccaca tccagccgcc agccgtccat
     9361 attccacggc gctttcagcc agtggcggac aatactgtct tccccgcgat aaatttcatt
     9421 caccagactt tccgactgat aatccagctt cgtcaagctg gcatagccaa gccagtcgag
     9481 cgccgtgcca tcatcactaa acgagtacca gtcgcgccag ggcgattcag agttgtgaca
     9541 agctcccccc gtgccaagat tgtgcctgtc aaaccaggca tgggaatcgc cactgtggtt
     9601 aaacacgccg tccagcacca gccgcattcc cagctgctgc gtattgtgtc gcaaacgcag
     9661 caacgcccca tcaccgccaa actgcggatc gacatggcga taatcctcgg tatcgtattt
     9721 atgtacgctg ggagctttaa acaccggatt gagatacagc gctgtcacgc caagcttttt
     9781 cagatacggc agtttttcgc ttatcccgtc cagatcgccg ccatagaacg ttgatccgcc
     9841 cgcctgcgcc gtgaccggtt catcccagtc acgcaagatg atctcttgtc cggctgcatg
     9901 atggtaatag acatgatcct gttcagcttc acgaggaaga ctacgcgcaa aacgatcagg
     9961 gaagatctga taaaaaatct gatccgcagc ccattgtggg ccgatatcag gtacatcgac
    10021 ggcaaactgc tccagtcgtg ccggcggcat tcggctgaag ccctgcggtg taaaccaacg
    10081 ctggcgatcg tgccacagca gtttgaaact gtaacgccgc cggggttgtc cgctggagag
    10141 atcaatcgcc gcacgccatg cggtgacgcc tggctgcggc tgactgcgct gcttatgcat
    10201 tggtactgac atttcttcgt tatcgtgttc tgtacgcagc ataatgcgct gcggtgggtc
    10261 ttcgcccgtc agccacagtg taatgagcag ttgatctttg ctttgtttaa caaatggggg
    10321 caccggcagg tgccatgcat ttaacatcat aattcccctg tgatgaaaat ctgatcacct
    10381 tgccatagcc taatgcagga tcactcatca tctgaaggat atttggggga ggaggacggg
    10441 ggcggataac tttcggaata gccggatcag gatttcctcc tgaaccggct tacagaacgc
    10501 aagtattatt ggttttcagc cagctgacga tcgtggcggc gtttaaacat ccagccaatc
    10561 aacagcacaa caatccacgc gaaaccgaca tacagcgaga tacgcgtatc cgggtgataa
    10621 ccaatcaacc cgataataaa gagcaggaaa atcagcccgc cgatggtcgt tgctacccca
    10681 cccggcactt taaatttcag cgccttaact tcttctggcg gcaaacggcg acggaaggcg
    10741 atttgcgaca gcaggatcat aatccacacc cataccgtgg cgaaggttgc cagcgaagcg
    10801 atcaccagga agacgttttc cggcatgatg tagttcagat acaccgcaaa cagcagcgcg
    10861 gtagtcatca ccagcaccgt aacccacgga ataccgcgac gcgacgtttt gctgaaaatt
    10921 ttcggtgcgc tgccctgctc tgccataccg tggagcatac ggcctacgcc aaatacgtca
    10981 ctgttaattg ccgacagcga agcggtcagc acaacaaagt taagaatgct ggcggcaaag
    11041 gtaatgccca tatgctggaa cgtcagtacg aacgggctac cggcagtgcc aacctgattc
    11101 cacgggtaga tagacataat gacgaacagc gtaccgacgt agaacaccag aatacgcatc
    11161 ggcacggagt taatcgcacg cggaatcgat ttctcaggat ctttcgcttc accggcggta
    11221 atcccgataa tttcgatccc accgtaagca aacatcacca tttgcaacga cattaccatg
    11281 ccaagccagc cgttactgaa gaagccgccg ttgctccaca ggttatgaat accggtcggt
    11341 tgcccgccgt tgccaatccc ccagatgatg atgccgaaac cggcgacaat catgatgatg
    11401 atggtggcga ctttaaagaa cgagaaccag aattccagct caccgaagac cttcacgctc
    11461 atcaggttta cggcgcaaat gatcagcacc acgctcagta cccaaatcca gtgcggcacc
    11521 gtcgggaaca agacacccat atagatacca aaagcggtca catcggcgat ggcgacaata
    11581 aggatttcaa aacagtaggt ccatccggta atgtaacctg ccagcgggcc aaggttttcc
    11641 tgcgcataac gcgagaaaga gctggcggcc gggttatgta ccgacatttc ccccagcgca
    11701 cgcataatga tatacgccgc gataccaccg ataatatagg ccaacaacac gctcggaccg
    11761 gccattttga tggcgtctgc cgaaccgtaa aacagcccgg tgccaattgc tgaacccagt
    11821 gccataaagc gtatgtggcg ggtacttagc ccacgcttta gcttattctt actttccatc
    11881 aattcctaac ccatcaacac aataaaaaac cacggggcat cgcagccccg tggttaaaac
    11941 aaatgttcag tgatttagtg agcgctggag gtcacctgac gacctgccgc gcgatcccag
    12001 ataatggcca gaaccaccat caccactgtt ggcattaacc acgccagacc ttgttcggcc
    12061 agcggtaaac gctgcgccca ggacggtaag atatcgctga atgcagatgc tttgatcccg
    12121 tcgagaatac caaaaagcag gctgataaac atcggcggag caatcacgcg ggacgaatta
    12181 tgccaccatg agcgtgtaaa acttaataca accagtgcga tacacggcgg ataaatagcg
    12241 gtcagcaccg gtacggagat ctggatcagc tggcttaagc cgaggttaga caccaccatc
    12301 gagaagccgc cgaggataaa caccagcgta cgataagaga gcggtacgta ctgggcaaag
    12361 aattctgcac aagcacaggt caggccaacc gccgtgacca gacaggcgat gaagattaac
    12421 gccgccagca ggaagctacc gccgccgcca aaggtgtgct gaacgtaagc atgcagaata
    12481 gcagcgccgt ttgcagactg atcgaccagc gacgcgctgt ctgaacccag acagaacagc
    12541 gccaggtaca gcaaagtcag accaacaccc gccatcaggc cagcccagac ggtataacgg
    12601 gtcagcagac gcgcttcggt aacgccacga gaacgcgccg cgttaacaat aacgatacca
    12661 aacaccattg cgcccagcgt atccatggtc agatagccgt taacgaagcc gttagaaaat
    12721 gcagcgtttt gataagcctc agtcgccgtg ctgatagaac ccgccggcca gacaatcgcg
    12781 acaacagaca ggatgaccag cgcgataatt ttcagcggcg caaggaagtt gcccacggta
    12841 tccagcagct tgcccggata gagcgaaacc agaataacga tagcgaaata gaccaggctg
    12901 tagataaaca gcggcagcgc ggaatcaccc gtcagcggcg caatccccac ttcaaaggaa
    12961 acggtagctg tacgcggcgt agcgaaaagc ggccccaccg ccaggtaaca aaccgttgcc
    13021 agcagtacgc cagcgacttt accgattggc gtgctgaggc tgtcaacacc gccgccaact
    13081 tttgccagcg ccactaccgt taataccggc aggccaacgg cagtaatgag gaagccgaat
    13141 gccgcagtcc agacgtgttc gcctgcctgt aagccgacca ttggagggaa aataatgtta
    13201 cctgcgccga cgaacaacgc aaatgtcata aagcccagag cgatgatatc gcgcgatctt
    13261 aattgatggg tcataaatca tactgcctgt ggatgtggtg ttgtaacgtt aaatttttgt
    13321 gccaatccct ggggatgata cagcggtata tttaaacaat tttagcggga aatactccca
    13381 accaggcgcg gcgaagaata gtttttcgat ttaccgcgtt aatgcagtca gtatgacagc
    13441 acctgaagcg caatttaaac gcttataacg ttttaaagca aggagggatc taaaaaccag
    13501 aggtatatag cgataagaaa tcaccaacca gtataaaaca ctatttccgt gaaaaagaca
    13561 tggctaatca tgcgaacaaa aaatcaccaa ccgttgtaaa atctaacgcc cgtattttga
    13621 ctgcaaagaa aataagccag catcggctgg cttatggcaa taaaagatga caaaggcgga
    13681 ttaatcgctg tttttggcaa ttaaacgttc cgggataaca aaactgaaac gtgttccttt
    13741 tcctactgta ctctcaatat tcaggcgact ttcgtgatga ttcaccgcat gtttcacgat
    13801 cgctaaccct aatccgctac cgccggtttg ccgggaacgc gctttatcaa cgcgataaaa
    13861 acgctcggtc aggcgcggaa tatgctccgg tgcaatgccc ggtccgttat cttcaacgct
    13921 aaattcggca ccgtgcggca ctcgctgcca gcgtacggtg atatgcgtgc cttccggcgt
    13981 atgattcacg gcgttataga ccaggttcga aatcgcactg cgtagctgat cttcgttgcc
    14041 agacaccttg aggccgttat ctatctcaaa ggtaaatgtc tgttttttct gactcagagt
    14101 ctgagcctcg cgctcaacaa cgcgcagcat catcggcaca tcaacctttt cattgagcaa
    14161 ttgcgtcggc gcggcttcaa ttttcgacag cgttagcaac tgcttcacca gcccttccat
    14221 ccgctgggtc tgctcgcgca tggtgtgcaa cgctttttcg cgtaccgcgc cttccagcgg
    14281 ctgctcatcc atcatctcca ggtaaccctg taacacggtc aatggcgtac gtaactcatg
    14341 gctcacgttg gcaaaaaagt tacgccgcgc cccttccagt tgatgcattt gcgtgacatc
    14401 acgcgccacc atcagcaact gtttgtgggt ataaggcatg acgcgaattt ccagatgccg
    14461 cccggtgttg agcaccagat tgagcgggcg agaaaaatca cgcgttttca gatattgcgt
    14521 aaactccggg taacgcagta ggttaaggat gttctgcccg ttatcttccg gccagcgcaa
    14581 accaagaatt tgttgcgcca gaccgttaca ccagaaaata ccgccctctt ccgtggtcag
    14641 caccaccgcg tcgggcagcg actccgcgcc gctacgaaag cgtttaatca gattgcccag
    14701 ttcacggcgg cgttttttat ttcgcagctg catctggtgt aagccgtata gtagcggttc
    14761 ccagctacca cgccccggtg gcggggtcat actgcgatcc acccacagcc accatgaaag
    14821 gcgcaataaa ttccagaaat gccagataag cagtcctgtt accgatgcca gcaaaaacca
    14881 gggcaggtaa ccaaaaaatg cacccaggat gaaagccggg aggcagcaaa gtagcagctc
    14941 cagcaccagc cttttccacg acagccgttc cagcacgtaa gatactccag ttaagaaatc
    15001 ataagccctg ctctgcgtcc gatgagcaag gcgttaaaag cgggttgaaa aacgatatcc
    15061 tgtaccgcgc acggtctgca ccatgcggtc atgcccgccg ggctccagtg ctttacgcag
    15121 gcgacgaatg tggacatcga ccgtgcggtc ttccacatac acgttagttc cccagacgtg
    15181 gtttaacagc tgctcgcggc tgtacacgcg ctcaggatgc gtcataaaaa agtgcagcag
    15241 tttaaattct gtcggcccca tctccagcgg ctcttcgccc gccatcactc ggtgagatgt
    15301 cgggttgaga cttaatccct gcatcttaat cacctcttcc accgccattg gcgaaatacg
    15361 gcgcattacc gctttgattc gcgccaccag ctccttcggc gaaaatggct tggtgatata
    15421 gtcatccgcg ccggtttcaa ggccgcgcac gcgatcttct tcttcccctc tggcggtcaa
    15481 catcaccact ggaatatccc gggtcatcga ctcgcgcttg aggtgtttga tgaactggat
    15541 accggagccg ccaggtaaca tccagtcaag gagaattaaa tccggccagg gttcattcag
    15601 ttgattcaca gcactgtcat aatcttccgc ttcgaccggc tgaaagccat tttgttcgag
    15661 cacgaagcag accatttcgc gaattggagc ttcatcttct acgaccagaa tacgtctcgc
    15721 catgatttgc cctgttgtaa taaataggtt gcgatcatta atgcgacgtc attatgcgtc
    15781 agatttatga cagatttatg aaaagctcgt cgcacatatc ttcaggttat tgatttccgt
    15841 ggcgcagaaa aaagcaaatg gcacatctgt ttgggtataa tcgcgaccat gctttttcgc
    15901 cagggaaccg ttatgcgcat ccttcacacc tcagactggc atctcggcca gaacttctac
    15961 agtaaaagcc gcgaagctga acatcaggct tttcttgact ggctgctgga gacagcacaa
    16021 acccatcagg tggatgcgat tattgttgct ggtgatgttt tcgataccgg ctcgccgccc
    16081 agttacgccc gcacgttata caaccgtttt gttgtcaatt tacagcaaac tggctgtcat
    16141 ctggtggtac tggcaggaaa ccatgactcg gtcgccacgc tgaatgaatc gcgcgatatc
    16201 atggcgttcc tcaatacaac cgtggtcgcc agcgccggac atgcgccgca aatcttgcct
    16261 cgtcgcgacg ggacgccagg cgcagtgctg tgccccattc cgtttttacg cccgcgtgac
    16321 attatttcca gccaggcggg gcttaacggt attgaaaaac agcagcattt actggcagcg
    16381 attaccgatt attaccaaca acactatgcc gatgcctgca aactgcgcgg cgatcagcct
    16441 ctgcccatca tcgccacggg acatttaacg accgtgggtt ccagtaaaag tgacgccgtg
    16501 cgtgacattt atattggcac gctggacgcg tttccggcac aaaactttcc accagccgac
    16561 tacatcgcgc tcgggcatat tcaccgcgca cagattattg gcggcatgga acatgttcgc
    16621 tattgcggtt cccccattcc actgagtttt gatgaatgcg gtaagagtaa atatgtccat
    16681 ctggtgacat tttcaaacgg caaattagag agcgtggaaa acctgaacgt accggtaact
    16741 caacccatgg cggtgctgaa aggcgatctg gcgtcgatta ccgcacagct ggaacagtgg
    16801 cgcgatgtat cgcaggagcc acctgtctgg ctggatatcg aaatcactac tgatgagtat
    16861 ctgcatgata ttcagcgcaa aatccaggca ttaaccgaat cattgcctgt cgaagtattg
    16921 ctggtacgtc ggagtcgtga acagcgcgag cgtgtgttag ccagccaaca gcgtgaaacc
    16981 ctcagcgaac tcagcgtcga agaggtgttc aatcgccgtc tggcactgga agacctggat
    17041 gaatcgcagc agcaacgtct gcagcatctt ttcaccacga cgttgcgtac cctcgccgga
    17101 gaacacgaag catgaaaatt ctcagcctgc gcctgaaaaa cctgaactca ttaaaaggcg
    17161 aatggaagat tgatttcacc cgcgagccgt tcgccagcaa cgggctgttt gccattaccg
    17221 gcccaaccgg tgcggggaaa accaccctgc tggacgccat ttgtctggcg ctgtatcacg
    17281 aaactccgcg tctctctaac gtttcacaat cgcaaaatga tctcatgacc cgcgataccg
    17341 ctgaatgtct ggcggaggtg gagtttgaag tgaaaggtga agcgtatcgc gcgttctgga
    17401 gccagaatcg ggcgcgtaac cagccggacg gtaatttgca ggtgcctcgc gtagagctgg
    17461 cgcgctgcgc cgacggcaaa attctcgccg acaaagtgaa agataagctg gaactgacag
    17521 cgacgttaac cgggctggat tacgggcgct tcacccgttc gatgctgctt tcgcaggggc
    17581 aatttgctgc cttcctgaat gccaaaccca aagaacgcgc ggaattgctc gaggagttaa
    17641 ccggcactga aatctacggg caaatctcgg cgatggtttt tgagcagcac aaatcggccc
    17701 gcactgagct ggagaagctg caagcgcagg ccagcggcgt cacgttgctc acgccggaac
    17761 aagtgcaatc gctgacagcg agtttgcagg tacttactga cgaagaaaaa cagttaatta
    17821 ccgcgcagca gcaagaacaa caatcgctaa actggttaac gcgtcaggac gaattgcagc
    17881 aagaagccag ccgccgtcaa caggccttgc aacaggcgtt agccgaagaa gaaaaagcgc
    17941 aacctcaact ggcggcgctt agtctggcac aaccggcacg aaatcttcgt ccacactggg
    18001 aacgcatcgc agaacacagc gcggcgctgg cgcatattcg ccagcagatt gaagaagtaa
    18061 atactcgctt acagagcaca atggcgcttc gcgcgagcat tcgccaccac gcggcgaagc
    18121 agtcagcaga attacagcag cagcaacaaa gcctgaatac ctggttacag gaacacgacc
    18181 gcttccgtca gtggaacaac gaactggcgg gttggcgtgc gcagttctcc caacaaacca
    18241 gcgatcgcga gcatctgcgg caatggcagc aacagttaac ccatgctgag caaaaactta
    18301 atgcgcttgc ggcgatcacg ttgacgttaa ccgccgatga agttgctacc gccctggcgc
    18361 aacatgccga gcaacgccca ctgcgtcagc gcctggtcgc gctgcatggg cagattgttc
    18421 cccaacaaaa acgtctggcg cagttaatgg tcactatcca gaatgtcact ctggaacaga
    18481 cgcaacgtaa tgtcgcactt aacgaaatgc gccagcgtta taaagaaaag acgcagcaac
    18541 ttgccgatgt gaaaaccatt tgcgagcagg aagcgcgcat caaaacgctg gaagcccagc
    18601 gtgcacagtt acaggcgggt cagccttgtc cactttgtgg ctccaccagc cacccggcgg
    18661 tcgaggcgta tcaggcgctg gagcctggtg ttaatcagtc tcgattactg gcgctggaaa
    18721 acgaagttaa aaagctcggc gaagaaggtg cggcgctgcg cgggcaactg gatgcattaa
    18781 cgaagcagct tcagcgcgat gaaaacgaag cgcaaagcct ccgacaagat gagcaagcac
    18841 ttactcaaca atggcaagcc gtcacggcca gcctcaatat caccttgcag ccacaggacg
    18901 atattcaacc gtggctggat gcacaagatg agcacgaacg ccagctgcgg ttactcagcc
    18961 aacgacatga attacaaggg cagattgccg cgcataatca gcaaattatc cagtatcaac
    19021 agcaaattga acaacgtcag cagcagctct taacggcatt ggcgggttat gcactgacat
    19081 tgccacagga agatgaagaa gagagctggt tggcgacacg tcagcaagaa gcgcagagct
    19141 ggcagcaacg ccagaacgaa ttaaccgcgc tgcaaaaccg tattcagcag ctgacaccga
    19201 ttctggaaac gttgccgcaa agtgatgatc tcccgcacag cgaagaaact gtggcgctgg
    19261 ataactggcg gcaggttcat gaacaatgtc tcgcattaca cagccagcag cagacgttac
    19321 agcaacagga tgttctggcg gcgcaaagtc tgcaaaaagc ccaagcgcag tttgacaccg
    19381 cgctacaggc cagcgtcttt gacgatcagc aggcgttcct tgcggcgcta atggatgaac
    19441 aaacactaac gcagctggaa cagctcaagc agaatctgga aaaccagcgc cgtcaggcgc
    19501 aaacgctggt cactcagaca gcagaaacgc tggcacagca tcaacaacac cgacctgacg
    19561 ggttggctct cactgtgacg gtggagcaga ttcagcaaga gttagcgcaa actcaccaaa
    19621 agttgcgtga aaacaccacg agtcaaggcg agattcgcca gcagctgaag caggatgcag
    19681 ataaccgtca gcaacaacaa accttaatgc agcaaattgc tcaaatgacg cagcaggttg
    19741 aggactgggg atatctgaat tcgctaatag gttccaaaga gggcgataaa ttccgcaagt
    19801 ttgcccaggg gctgacgctg gataatttag tccatctcgc taatcagcaa cttacccggc
    19861 tgcacgtgcg ctatctgtta cagcgcaaag ccagcgaggc gctggaagtc gaggttgttg
    19921 atacctggca ggcagatgcg gtacgcgata cccgtaccct ttccggcggc gaaagtttcc
    19981 tcgttagtct ggcgctggcg ctggcgcttt cggatctggt cagccataaa acacgtattg
    20041 actcgctgtt ccttgatgaa ggttttggca cgctggatag cgaaacgctg gataccgccc
    20101 ttgatgcgct ggatgccctg aacgccagtg gcaaaaccat cggtgtgatt agccacgtag
    20161 aagcgatgaa agagcgtatt ccggtgcaga tcaaagtgaa aaagatcaac ggcctgggct
    20221 acagcaaact ggaaagtacg tttgcagtga aataactatt cagcaggata atgaatacag
    20281 aggggtgaat tatctcttgg ccttgctggt cgttatcctg caagctatca ctttattggc
    20341 tacggtgatt ggtagccgtt ctggtggttg tgatggtggt atgaaaaaag tcattttatc
    20401 tttggctctg ggcacgtttg gtttggggat ggccgaattt ggcattatga gcgtgctcac
    20461 ggagctggcg cataacgtag gaatttcgat tcctgctgcc gggcatatga tctcgtatta
    20521 tgcactggtg gtggtggtcg gtgcgccaat catcgcactc ttttccagcc gctactcact
    20581 caaacatatc ttgttgtttc tggtggcgtt gtgcgtcatt ggcaacgcca tgttcacgct
    20641 ctcttcgtct tacctgatgc tcgccattgg tcggctggta tccggctttc cgcatggcgc
    20701 attttttggc gtcggagcga tcgtgttatc aaaaattatc aaacccggaa aagtcaccgc
    20761 cgccgtggcg gggatggttt ccgggatgac agtcgccaat ttgctgggca ttccgctggg
    20821 aacgtattta agtcaggaat gctggcgtta caccttttta ttgatcgctg tttttaatat
    20881 tgcggtgatg gcatcggtct atttttgggt gccagatatt cgcgacgagg cgaaaggaaa
    20941 tctgcgcgaa caatttcact ttttgcgcag cccggccccg tggttaattt tcgccgccac
    21001 gatgtttggc aacgcaggtg tgtttgcctg gttcagctac gtaaagccat acatgatgtt
    21061 tatttccggt ttttcggaaa cggcgatgac ctttattatg atgttagttg ggctagggat
    21121 ggtgctggga aatatgctaa gtggcaggat ttcaggacgt tattcaccac tgcgcattgc
    21181 agcagtgact gactttatca ttgtactggc actgctgatg ctctttttct gcggcggcat
    21241 gaaaacaacg tcgcttattt ttgcttttat ttgttgcgcg ggattatttg ccctttcagc
    21301 acctctgcaa atattgttac tgcaaaatgc caaaggagga gagttattag gtgccgcagg
    21361 tgggcaaata gcgtttaacc tcggtagcgc cgtcggcgca tattgcggag gtatgatgct
    21421 gacgctgggg ctggcatata attacgtggc gctgcctgcc gccctgcttt cgtttgctgc
    21481 gatgtcatcg ttgctgctgt atggtcgcta taagcgccag caagcggcgg atagtccggt
    21541 gctggcgaaa ccactggggt agattactgt cacggtgcgc gttgcttatt gccggatgcg
    21601 gcgtgaacgc cttatccgcc ctacgcggtt ctggcacatt ttgtaggcct gataagacgc
    21661 ggcaagcgtc gcatcaggca tgatgcgcca attgcctacg ttttttactc ttgtggccat
    21721 aaccacgcag cgccgcgtac gccgctggaa tcaccgtgct tcgccttact caccggcgtt
    21781 tcacattcgc cgccgaagac aaattgttta atcaactgcc caaccgtttg atataaacgg
    21841 tctacattgc tcatcccgcc ccccaggaca atcacatccg gatcgagaat attcacgaca
    21901 tgtgccagcg attttgccag ccgcagctcg tagcgacgca atgccagttc cgctaccgga
    21961 tcgctttctt caaccaggcg gataatttca ctgcctttca gcgcatgtcc gctcaaacga
    22021 cgataatccg tcgcgaatcc cgtgcccgaa ataaaggttt caatacaacc ttgtttaccg
    22081 caataacaag ggacttcctc gcgataacgc agttcgtctt cgtccatcca cggtagcgga
    22141 ttgtgtcccc actcacctgc cgtgccattg ccgccgatat gcgtccgccc attgaatgcc
    22201 acgcccgcgc cgcatcccgt gccgataatc acggcaaata ccgtctgcgc tcccgctgcc
    22261 gcgccatcta ctgcttctga aaccgccaga cagttggcat catttgccag ccgcacttcc
    22321 cgctgcaacc tcgcgcttaa gtctttatcg aatggctgac cgttgagcca ggttgaattg
    22381 acattcttca ccacaccggt gtagggcgaa atcgaacctg gaatgcccat acctaccgtt
    22441 ccgcgctgcc ctgtcgcctg ctccgccata tcaaccaacg tggcgatcgt ttcaatagtc
    22501 tgccggtaat catcacgcgg cgtgggcaga cgatggcggt acaactgctc ccctgcatcg
    22561 cccagtgcaa tcacttcagt tttggtgccg cctaaatcga tacctatacg cactgtactc
    22621 tccttatttt tttcaatatc aatagcgtag agacggacaa ccggattggc aatgcaaggc
    22681 cgccgacaat tcgttatcat gcccgctaaa tttaacgaca aggccgtgga aattatcatg
    22741 ctgtggttca aaaatttaat ggtttaccgt cttagccgcg agatttcgct gcgtgcagaa
    22801 gagatggaaa aacagctagc ctcgatggca tttaccccat gcggcagcca ggacatggcg
    22861 aagatgggct gggttcctcc gatgggatcg cacagcgatg cgttaacgca cgttgccaat
    22921 ggtcaaattg ttatctgcgc gcgcaaagaa gaaaaaatcc tcccgtctcc ggtgattaaa
    22981 caggcgctgg aagcgaaaat cgccaaactg gaagcggaac aggcgcgtaa gctgaagaaa
    23041 accgaaaaag attcgctgaa agacgaagtg ctgcactctc tgctgccgcg tgctttcagc
    23101 cgttttagcc agacaatgat gtggctcgac acggttaacg gtttgattat ggtggactgc
    23161 gccagtgcca aaaaagcgga agatacgctg gcattactgc gtaaaagcct ggggtcgtta
    23221 ccggttgtac cattgagcat ggaaaacccg attgaactga cgctgaccga atgggttcgc
    23281 tccggtagtg cggcacaggg cttccagctg cttgatgaag ccgagctgaa atcgttgctg
    23341 gaagatggcg gcgtgatccg cgcgaagaaa caagatctga ccagcgaaga gatcaccaat
    23401 cacattgaag ccggaaaagt ggtgactaaa ctggcgctcg actggcagca gcgcattcag
    23461 tttgtgatgt gcgacgatgg ttcgctcaag cgtcttaagt tctgcgacga gctgcgcgat
    23521 caaaacgaag atatcgaccg tgaagatttc gcccagcgtt ttgatgccga tttcatcctg
    23581 atgactggtg aactggcagc gttaattcaa aacctgattg aaggattagg tggcgaagca
    23641 caacgttaat tgctgatttt cctttaatgc cggatgcgac gcctgccgcg tcttatccgg
    23701 cgtacgaagc cacaccaggc atataattat tcgctacggc gagcaataat ttttagcgca
    23761 gcaatattat gcgttttacg ccgtaactga tctaatcgcc tcatccctat tcgcctgtca
    23821 gcagcacgca gaatattcac taacccccat ttatcgctgg taaattgttt tgttaacagc
    23881 tccgcttttg gggtatcaat atattcacgc aataaatcag aaattgaaca aatatccgtc
    23941 aggtaaggat accagcaagt ttcaccgtga aaattacgca ctccagcgtt acctgcaata
    24001 aagtctttcg ggtaatccca gatcacgttt ttatccaacg tgatccaata acgcggtaaa
    24061 tcggtacttc caccattttg gctgcgcatg ggataacggg tacagtgaat ttgcaggttg
    24121 atcgttggcg tcagtaagtc atagatctcc cgctgtaact tgctccatgg acgttgtgtc
    24181 attgtttttc ctcaagccgg tgacgcgctt cactgcgaat tttccgcgtt gccccggttt
    24241 tcactaaatc acgtaactgc gaaagatcgg gttgttcgac aaataagagt aatgatggat
    24301 cctcttttaa ccacgccgtt ttcacatcgg cagccagctg tggattatta atagccagcg
    24361 accgatcctg aggctcagtc aatgtcgtta acaacgacgc cggggtatgt gcgttgcgta
    24421 atgccgtttg gcgcaaatga tcattgcgac tttgcagcaa tgcacgccac cattcatcat
    24481 ctgatttatc ttttatcgta gccagttccg caaaaaatac ctggagatct tcactgatat
    24541 ttatatcaac cttgatgaat tatgaatata tcggaaattg aaaaaatcgc agcaaaggaa
    24601 atattattat gggttaaaga gaggatattt cagaaaaatg ccctcccgtt caaggggaag
    24661 gcgaggaatt acagatagcg gcacagataa gaggtaggtt cggcaacttg aagatgaaac
    24721 tcactgtgac cgggaacatt aaacaccgaa ccggcttcat acacttgcca gtcggtcgcg
    24781 tcaggcagta acacattcag cgcgccactg attaccgtca tctcttccgg ctcagcggtg
    24841 ctgaaggtgt attcgccttc aaccataaca cccacgctgg cgcgaccagt gctgctgctg
    24901 gaaaagccga tcgatttcac tttgccggaa aagtactcat tactttgaag cataaactgg
    24961 ccctgtctgg tagttgaaca tctacttcac tataggggcc agaggcgctg gctgtcacgc
    25021 aaaattacac cagtaattcc gcagccagcc gtgcaatcaa tacgttagag agcaagacag
    25081 gaacatcgag ctgtttttgc agtaaatcgc gatgacgttg gtgaaatcct aaacaatcca
    25141 gcatgatgac atcagcccct tttgccagta attctttccc agcatcaatg attttttgtt
    25201 ctgaatcatg aatggggtta cccaatgaaa ataccggcga tttctgcaaa atttgccatt
    25261 tttgcgcctg aacgggcagc atctcctcaa ccggaacgat aacccccacc tgatgatctt
    25321 caacaataga ggaaaccagt ggaggcaata ttcgcgacgg ctcaagaaag atcgtattac
    25381 gcgcagtcat actactaatg tttgctgtac tcattaataa aatgacgtca taaccctgat
    25441 tatcgagcac ttcaaccaca ccttgcagat ctcgctccac tttacgacgc gaaacatggg
    25501 ccagctggtt gtcatttaat aatgtgagaa tggtgtcttc gcctgcttct ggcgcgtact
    25561 ccgccatcac ttcttcacga cttaacttcc ccagcaggct atgatgggaa atattatctt
    25621 cgtcaatgta ttccgtcagg agcggcaaaa cctcctgcat aggtacaatg ccgatggtca
    25681 ggatcgccaa cgacgcactc attgttcatc caccttactt ttctttcgtc gttaattacc
    25741 gggcaagtgt gaagcaccac tgctgacact tacttgtgct acaaatgaca aaaagcgtag
    25801 cagcagacgt gcacggcata atgtaaagat tccaaatgat tccagtaatg gaatgttatt
    25861 gtttaatatt ctaattatta gaaaaacata aattatgaaa aatgtgacgc agatcccttg
    25921 tagtggcact cagaatccct tccgagcagt ctgatgcggt cactctttgt cagggtcctc
    25981 atagcgtttt ttcgcatcca tcgcttcctg agccgtcgga tgttcactga tcaacgagtc
    26041 tggtttagga tgatcggctc tgagttggta ccaggttacc gtctgtcctg gctttccttt
    26101 ttcaatcgtc actatatatg cttcacgagg ataaggcggt ttcgttggca tcgttcttcc
    26161 ttatttcacg ggatgaacgt taagtatagg cgctctaaaa tcaacaattg atcgtctgtg
    26221 ccagggcgct acgaatttca gaaatcacct ggctgggttc gtttgttgcg tcgatgataa
    26281 tatgcgcaac ttcgcgatat agcgcatcgc gttcttccag cacttcctga acttcttcgc
    26341 tcagcggttt tcccgttaag gttggccgta aatcttcttc cggtgcagct tgcagtcggt
    26401 taaccaggac tgatactggc gcacacaaat aaaccacgat cccgttattt tgcatgaagt
    26461 gacgattaaa ttccgtcaga ataatgccgc cgcctgtagc gataacggtg gatgccgcag
    26521 ttaccgcttc cagcgccgcc gtttctctgg cgcgaaatcc cgcccactct tccctttcga
    26581 cgatctccgc gaccgtcata ttgagctgtg attgcaacca ctgatcggta tcgacaaacc
    26641 gacggttaag cgaatcggca agggccattc cgaccgttgt tttaccacag ccccgaggcc
    26701 cgatcagaaa aagaggttgt gtcatcgtgg gttttcccca ataggtcgca atgcggcgaa
    26761 agccggtgtc atgagaatag cgatcatacc atcaaactag tacaatttcg attgtaaaga
    26821 aaaaattcca cttaaagtaa aaatctcaat ac
//
LOCUS       DAZXUL010000055        25703 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZXUL010000055 DAZXUL010000000
VERSION     DAZXUL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25703
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.055"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1332
                     /gene="yjeM"
                     /locus_tag="ACH3L1_002264"
                     /pseudo
     CDS             1..1332
                     /gene="yjeM"
                     /locus_tag="ACH3L1_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="ACH3L1_002265"
     CDS             complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="ACH3L1_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFT4076981.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4816..5784)
                     /gene="asd"
                     /locus_tag="ACH3L1_002266"
     CDS             complement(4816..5784)
                     /gene="asd"
                     /locus_tag="ACH3L1_002266"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFT4076982.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="ACH3L1_002267"
     CDS             complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="ACH3L1_002267"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFT4076983.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7028..7573
                     /gene="orn"
                     /locus_tag="ACH3L1_002268"
     CDS             7028..7573
                     /gene="orn"
                     /locus_tag="ACH3L1_002268"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFT4076984.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7784..7859
                     /locus_tag="ACH3L1_002269"
     tRNA            7784..7859
                     /locus_tag="ACH3L1_002269"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7817..7819,aa:Gly,seq:gcc)
     gene            8017..8092
                     /locus_tag="ACH3L1_002270"
     tRNA            8017..8092
                     /locus_tag="ACH3L1_002270"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8050..8052,aa:Gly,seq:gcc)
     gene            8128..8203
                     /locus_tag="ACH3L1_002271"
     tRNA            8128..8203
                     /locus_tag="ACH3L1_002271"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8161..8163,aa:Gly,seq:gcc)
     gene            8239..8314
                     /locus_tag="ACH3L1_002272"
     tRNA            8239..8314
                     /locus_tag="ACH3L1_002272"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8272..8274,aa:Gly,seq:gcc)
     gene            8548..8601
                     /locus_tag="ACH3L1_002273"
     CDS             8548..8601
                     /locus_tag="ACH3L1_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4076985.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(8584..9723)
                     /gene="queG"
                     /locus_tag="ACH3L1_002274"
     CDS             complement(8584..9723)
                     /gene="queG"
                     /locus_tag="ACH3L1_002274"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFT4076986.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            9722..11269
                     /gene="nnr"
                     /locus_tag="ACH3L1_002275"
     CDS             9722..11269
                     /gene="nnr"
                     /locus_tag="ACH3L1_002275"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFT4076987.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            11241..11702
                     /gene="tsaE"
                     /locus_tag="ACH3L1_002276"
     CDS             11241..11702
                     /gene="tsaE"
                     /locus_tag="ACH3L1_002276"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFT4076988.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            11721..13058
                     /gene="amiB"
                     /locus_tag="ACH3L1_002277"
     CDS             11721..13058
                     /gene="amiB"
                     /locus_tag="ACH3L1_002277"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFT4076989.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            13068..14915
                     /gene="mutL"
                     /locus_tag="ACH3L1_002278"
     CDS             13068..14915
                     /gene="mutL"
                     /locus_tag="ACH3L1_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFT4076990.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            14908..15858
                     /gene="miaA"
                     /locus_tag="ACH3L1_002279"
     CDS             14908..15858
                     /gene="miaA"
                     /locus_tag="ACH3L1_002279"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFT4076991.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            15944..16252
                     /gene="hfq"
                     /locus_tag="ACH3L1_002280"
     CDS             15944..16252
                     /gene="hfq"
                     /locus_tag="ACH3L1_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFT4076992.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            16328..17608
                     /gene="hflX"
                     /locus_tag="ACH3L1_002281"
     CDS             16328..17608
                     /gene="hflX"
                     /locus_tag="ACH3L1_002281"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFT4076993.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            17694..18953
                     /gene="hflK"
                     /locus_tag="ACH3L1_002282"
     CDS             17694..18953
                     /gene="hflK"
                     /locus_tag="ACH3L1_002282"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFT4076994.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            18956..19960
                     /gene="hflC"
                     /locus_tag="ACH3L1_002283"
     CDS             18956..19960
                     /gene="hflC"
                     /locus_tag="ACH3L1_002283"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFT4076995.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            20042..20239
                     /locus_tag="ACH3L1_002284"
     CDS             20042..20239
                     /locus_tag="ACH3L1_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFT4076996.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            20343..21641
                     /gene="purA"
                     /locus_tag="ACH3L1_002285"
     CDS             20343..21641
                     /gene="purA"
                     /locus_tag="ACH3L1_002285"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFT4076997.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            21846..22271
                     /gene="nsrR"
                     /locus_tag="ACH3L1_002286"
     CDS             21846..22271
                     /gene="nsrR"
                     /locus_tag="ACH3L1_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFT4076998.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            22310..24751
                     /gene="rnr"
                     /locus_tag="ACH3L1_002287"
     CDS             22310..24751
                     /gene="rnr"
                     /locus_tag="ACH3L1_002287"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFT4076999.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            24931..25662
                     /gene="rlmB"
                     /locus_tag="ACH3L1_002288"
     CDS             24931..25662
                     /gene="rlmB"
                     /locus_tag="ACH3L1_002288"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFT4077000.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT         6019 a         6515 c         7217 g         5952 t
ORIGIN      
        1 cttaatgatg gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta tctattcctg
       61 gatgaataat agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc
      121 ttatatcatc tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct
      181 ctatggtagt gacatgaccc agcactggcg tattgccgga ctggagccta cgcaggtggt
      241 tggtctgctg gcagtagcat ggatgattct ggtcacagtc gttgcttcaa aggggattaa
      301 taaaattgcc cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt
      361 gctgttagta agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa
      421 tttccttgca tcaccgaacc cgggttatca gtccggtctg gcaatgctat cgtttgtggt
      481 atttgctatt tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga
      541 aaatccagaa aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg
      601 ttattcgctg gcaatatttt tatggggcgt cagcacaaac tggcagcagg tattaagtaa
      661 tggttccgtt aaccttggca atattaccta tgtgctgatg aagagcctcg gggtgatgct
      721 gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat ggtttgcgcg
      781 tattaccgga ctttcgatgt tcctcgctta taccggtgcg tttgctattc accgctgaaa
      841 gccatcatcc aggggacgcc gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg
      901 atggggatgc cgtctatcgc catgtggatg cagtgcgggt tggttactgt cttcattctg
      961 ctggtttcgt ttggtggcgg taccgcatcg gcgttcttta acaagctgac gctgatggcg
     1021 aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg ctttcccgtt ttttaaagca
     1081 cgtcaggatc tcgacagacc atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg
     1141 acggtggttg tcgttctggt ggtgacattt gcgaacgtct tcaccattat tcagcctgtg
     1201 gttgaagccg gagactggga cagcacattg tggatgattg gcggccctgt cttcttctcg
     1261 ctgttagcga tggcgattta ccagaactat tgcagtcgca tggcgaataa acctgagtta
     1321 gctctcgact gatcatatga aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag
     1381 gaagtaatgc gtatggatga tactctccag ggccggtgat aactgaagat gatgttaagc
     1441 tgctaaatga gccacgttga catcatttgg cagcttcatg cattacaaac ttcccgcctg
     1501 acgacctttg cccgcagacg tcagcgttct ccccgtttgt ttaccgttga ggctttccag
     1561 acgcatctgg aagggcggga atggcatatc gataccgtgg gcgtggaagc ctgccagaat
     1621 cagctggtgg atctcatggc gtagcggcat acggtgaccc atctcagcgg cgtaaatacg
     1681 cagctcgaaa atctgaatcc cctgttgcag atccaccagg aagacttccg gtgccgggtt
     1741 gtcgatcacc agcgagcagc gacgcgctgc ggtgagcagg atttccgtca cttcttcact
     1801 gttggcatcg gcaggggccg gaatcgtcaa cactacgcgc gtgaccgagt cagagagcga
     1861 ccagttgata aactgctcgg taataaacgc cttgttcggc acgattatct ctttacggtc
     1921 ccagtcgctg atggtggtgg cgcgggtgtt aatttttgtc acgctaccag taagatcgcg
     1981 gatcgttacc gtatcgccaa tgcgaatcgg tttttcgaac aggatgatca ggccagagat
     2041 aaagttggcg aaaatttcct gcaaaccaaa accgagacca acaccgagcg cggcaaccag
     2101 ccactgcaat ttcgaccact caataccaat cattgagaag ccgaccagcc cgccaatcag
     2161 catcagcaga tattttgtga tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag
     2221 gtgctgcaaa atcgccagtt ccagcagcgc gggcaggttg cgcaccagct gcgtggtgat
     2281 gataaatacc agaatggcaa tcagcaccgc accgagggta attggctcca gactttctac
     2341 gccctgtacc gtggaggtga catcccacag cgaaatattt tcgaggaagc cgaaagcgga
     2401 atggatttct gaccacagca caatgaccga aagcagggcg atcaacatca aaattgagcg
     2461 caccagccgc aaggattgcg cactgatggc atcgagatcg acttcgcttt catcgacttc
     2521 aattgctcct tccgggctac tgtgatgatg cgcttcctct tcgccacgcg cacgttgcgc
     2581 taacatctct gcccggcgat gcttcgcccg gtcaaacgcc agcctgcgac gctggatcag
     2641 catccagcgg cggataacgt gataaaccac cagtagcagg aaccagatgg caaccgaggt
     2701 ttcaagcctc gctaacagtg cctgcgccgt tgccagataa cccaccgccg acgccagaat
     2761 ggcaaccagt ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa tgttgtcgcc
     2821 gctgccttct ttgttgagat acaacggaat accggctttt ttcaggctta gggtgaccac
     2881 cgccagcgca ccgcaaatga ggataaagca aagccgtccc agcgaaccgg aaaattcgcg
     2941 gtcgtcgagg ttatcgaaca tcatcagcgc cataatcagc ccgatgctca tcaggtagta
     3001 gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt
     3061 cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg tggccgttac
     3121 accatcgcca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc cgtaacccag
     3181 cgtcatccac agcaccggta acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa
     3241 cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa
     3301 acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaaacag
     3361 cggcaaaatt gtctctttgc tggtcagcat catcacactg gctttgccca actgactgaa
     3421 ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagcggcc aggcgatggt
     3481 catcgggcgc acgtcagagg cccagaacag atagcggtgc gttgcttcgt tcacctcttt
     3541 cagcgcatcc tccagttgcc tgttggagac tttcagcttg gtcagttcca atagtagcgt
     3601 gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag
     3661 aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatggattt gccgtagcag
     3721 cggctgttta ttaagtaaat cctcataacg taaccgttgc acacgcaact gcgccatttc
     3781 ggtatcaagc tgttgtggtt ttggcatttc tggtagccgt gccacctgcg cccgcagcgc
     3841 ttcgccgagc aggttggacg atcccagcca ttgcgactgt tcacgcagcg tattcaacgc
     3901 ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt tgcgaggcaa cgagctccat
     3961 ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg ttaattttga attgcgcgac
     4021 gatatctttc ggcaaatcgg cactgttttc tgccagtaat tcggtacttt ccagcgcccg
     4081 ctccgcctca agctgacgtt ggctattcaa ctgattacgt aaggcctgca aatacgcatc
     4141 cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt
     4201 attggcagac agctgcgcca gctccagttc atcaacgagc gccttaagac gtgcagagtc
     4261 agactgcaac gcgaaatttt gtgcctgatt gagcggagta ttgccggtaa gcgttcccag
     4321 gcggcgctcg atctcattta actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt
     4381 cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc
     4441 cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acattcccgg
     4501 cgacacgctg cgcggctcgt cacgaatgtt gtttaattgt gcgcgcagag tagcggagag
     4561 tttcggataa ttatcgataa cttgctgata ttgtttgatg cgctcaaggg aaccttttcg
     4621 ttcctcaagc gcatttaagg cagactggag cgcctctacg acttccggct gtgcgggttt
     4681 cgccgctttt gcctgctcca gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc
     4741 gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc
     4801 gtttcctttg atggattaga cctggtcttt tttatcgtca accaatgggc tggcgtcgtg
     4861 ttctgcttcg atctcttcag caggaagtgg ggcgggttca gcgtctggcg taacaaaggt
     4921 ttcggtagat actgccagcg gctggccaat tttcgtgaca gacaggcttt ccagttgctc
     4981 aaccagattc actttacccg gtgcaaacag gttgataacg gtggaaccga gtttaaagcg
     5041 acccatttcc tggcctttca gcagtgccac agaaccgtcg ttttccccgg caggccaggt
     5101 ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat
     5161 gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa
     5221 aaggcaaatc acgcgttcgt tacgggcaaa cagatttggc acgttctgag cggtgagatg
     5281 gttaacggaa aagagatcgc ccggcacgta gatcatctca cgcagaatac cgttgcacgg
     5341 catgtgtacg cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg
     5401 gaacaggtcc gccatcagat agttgcctgc cagcagggct tcgaggctgt agttgtggcc
     5461 tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc
     5521 aggcatgacc agtacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg
     5581 gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat
     5641 gtcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg
     5701 cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag
     5761 cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc
     5821 cggccaacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg
     5881 tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat
     5941 acggtgatag ttttcgaaac gggtttccgc gattttccct tcctcaaccg cttcccggat
     6001 agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaaatagtc
     6061 atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc
     6121 acgcacacca ggggaatcaa tcacatcacc gccgtgcggg aagtgataca gccgagcggc
     6181 ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca tcgttggtca ggatctcttt
     6241 ttgcagcccc agcaacgcgt tcagcaggct ggatttaccg acgccagact gcccggcaaa
     6301 aatgctgatg cgcccggtca acgcctcttc cagcggtttc agcccatcct gagtatggct
     6361 ggaaaccatc aatacgcgat aaccgatgtt gcggtagata tccatctgct cgttgacgaa
     6421 cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc acgataatcg gctcaatctg
     6481 caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat
     6541 ggcggagaca atgacaatct ggtcgatgtt ggcggctatg ggtttcacgc cgtcgtagaa
     6601 atccggacgc gttaacaccg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt
     6661 tacgccttcc gccgccggtt tacccggacg ccagactacg cggtcgccgg ttaccagcga
     6721 acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc
     6781 gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc
     6841 gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg
     6901 ctgctggcct ttggagagtt tatttttact caatcgtact gactcctggt cgcccctgat
     6961 gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga
     7021 aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct
     7081 ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaatctgaa
     7141 tattctggca gaagggccga ccattgcagt acatcagtcc gatgaacagc tggcgctgat
     7201 ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtagagcgcg tgaaagcgag
     7261 cacgatgggc gatcgggaag ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc
     7321 tgcgggaaaa tcgccgattt gcggtaacag catcggacag gaccgtcgtt tcctgtttaa
     7381 atacatgccg gagctggaag tctacttcca ctaccgttat ctcgatgtca gcaccctgaa
     7441 agagctggcg cgccgctgga agccggaaat tctggatggt tttaccaagc aggggacgca
     7501 tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg gcttactacc gcgagcattt
     7561 tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg
     7621 attttgacgc taaatcgaac gtcttgctga taatttcagc acttgagata aaaacgcaaa
     7681 aaaaactttt ttggggggtt gcagagggaa agatttctcg tataatgcgc ctcccgtaac
     7741 gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag tacgcgggaa tagctcagtt
     7801 ggtagagcac gaccttgcca aggtcggggt cgcgagttcg agtctcgttt cccgctccaa
     7861 aatttgaaaa gtgctgcaaa gcacagacca cccaatcgca gtatttcgaa ttgcgacaag
     7921 gcggcaactg aacgaatcct tgggaactta caaaagtatg tgactaaggt gagtgaaggc
     7981 agccaaggca gtagcggttc gaaagacgaa gattaagcgg gaatagctca gttggtagag
     8041 cacgaccttg ccaaggtcgg ggtcgcgagt tcgagtctcg tttcccgctc caaaatttaa
     8101 aagtgctgta aggcatagac cacccaagcg ggaatagctc agttggtaga gcacgacctt
     8161 gccaaggtcg gggtcgcgag ttcgagtctc gtttcccgct ccaaaatttg aaagtgctgt
     8221 aaggcacaga ccacccaagc gggaatagct cagttggtag agcacgacct tgccaaggtc
     8281 ggggtcgcga gttcgagtct cgtttcccgc tccaaattct tctctcaata aaatatctac
     8341 agcgacgcga tgcgttattg ctggtttttg ttgtctctga caaactcttg taaacagagt
     8401 tatccacagc ctcaggctgt aatcttaatt tcaaagaaac ttcgcacggt gaatggtatt
     8461 tttttaacct attgataaat aagttaaaaa tcaagattcc gttttgtcga gtcgatcact
     8521 tgacgatttt attcgtcttg aattgcaatg cgtttttatt tttattcaca agctgtggat
     8581 gaatcaggcg tcacgcggta accctttttc aatcacccga accagacgct gttttttcgg
     8641 caattgcact tcgactatgc acgcatttcg tctctctatt tgctgcgcaa tcgcccacgc
     8701 tatgtgctca tcgagaagtg ggtgctcacc tttacgactt tccagcgctg tcaaaatcgt
     8761 ttcatcccaa ggggcattgc ctaatgccac ggcgatatta cgcagccagc gcaggtgacc
     8821 tatacggcga atcgccgatc cttccgtgac ttttaaaaac ttctcttcgc tccaggcaaa
     8881 taactcaatg agttccggtg cgtgtagcgg cttacgcggg ctgaaatcgt cttctgtagt
     8941 gagttgcgaa tagcgattcc acgggcagat aagctggcag tcatcgcaac cgtaaatacg
     9001 gtttcccatt aacggtcgca actcttccgg gagcgcccct tcaagttcga tggtgagata
     9061 agagatacag cggcgagcat cgacggtata tggctcgacg atggcaccgg tcgggcaaat
     9121 cgtcatacag gccacgcatt tgccgcatcc ttcctcgact ggttgatcca cgggcagcgg
     9181 aatatcgacc agcaattcgc ctaaaaagaa gaacgaaccg gcctcgcgat tgaggataag
     9241 tgagtgctta cctgtccagc cgagcccagc tttttcagct aacgggcgct cgagaatagg
     9301 cgcagaatcg acaaacggtc taaaattcag cgaaacacaa tgttgctgaa tcatctcgcc
     9361 cagctttttg agtcggttgc gcagaagttt gtgatagtca cggcccagcg cataacggct
     9421 aacatagccg agtttggggt ttttcagcgt gctggcaaat gcggcgttag caggaaggta
     9481 attcatccgc acgctgatta cacgcagcgt accgggtaat aactcatggg ggcgagcgcg
     9541 cagcataccg tgacgtgcca tccagtccat ttcgccgtgg tattgtttgt ccagccatgc
     9601 ttgcagtttg ggctcggact cgctgagatc ggtatcggta atacctacct gctgaaagcc
     9661 cagttccagc ccccactgtt taattttttg cgctaactga ttgagatcga ggggctctga
     9721 catgacggac catacaatga agaaaaaccc cgtaagtata ccacacaccg tctggtacgc
     9781 cgacgatatc cgccgcggag aacgcgaggc ggcagatgtg ctggggctca cactctatga
     9841 gctgatgctt cgcgctggcg aggccgcatt ccaggtgtgt cgttcggcgt atcctgacgc
     9901 ccgccactgg ctggtgctgt gcggtcatgg taataacggc ggcgatggct acgtggtcgc
     9961 gcgactggcc aaagcggtcg gcattgaggt cacgttgttg gcccaggaga gcgacaaacc
    10021 gttgccggaa gaggccgcgc tggcacgcga agcatggtta aacgcgggtg gcgagatcca
    10081 tgcttcgaat attgtctggc ccgaatcggt agatctgatt gttgatgcgc tgctcggtac
    10141 cggcttgcag caagcgcccc gcgaatccat tagccagtta atcgaccacg ctaattccca
    10201 tcctgcgccg attgtggcgg ttgatatccc ttccggcctg ctggctgaaa ccggcgctac
    10261 gccaggcgca gtgatcaacg ccgatcacac catcactttt attgcgctga aactaggctt
    10321 gctcactgga aaagcgcggg atgttacagg acaactgcat tttgactcac tggggctgga
    10381 tagctggctg gcaggtcagg agacgaaaat tcagcggttt tcggcagaac aactttctca
    10441 ctggctgaaa ccgcgtcgcc cgacttcgca taaaggcgat cacgggcggc tggtgattat
    10501 cggtggcgat cacggcacgg cgggggctat tcgtatgacg ggggaagcgg cgctacgtgc
    10561 tggtgctggt ttagtccgag tactgacccg cagtgagaac attgcgccgc tgctgactgc
    10621 acgaccagaa ttgatggtgc atgaactgac tatggactct cttaccgaaa gcctggaatg
    10681 ggccgatgtg gtggtgattg gtcccggtct gggccagcaa gagtggggga aaaaagccct
    10741 gcaaaaagtt gagaattttc gcaaaccgat gttgtgggat gccgatgcat tgaacctgct
    10801 ggcaatcaat cccgataagc gtcacaatcg cgtgatcacg ccgcatccta gcgaggccgc
    10861 acggttgtta ggctgttccg tcgctgaaat tgaaagtgac cgcttacatt gcgccaaacg
    10921 tctggtacaa cgttatggcg gcgtagcggt gctgaaaggt gccggaaccg tggtcgccgc
    10981 ccatcctgac gctttaggca ttattgatgc cggaaatgca ggcatggcga gcggcggcat
    11041 gggcgatgtg ctctctggta ttattggcgc attgcttggg caaaaactgt cgccgtatga
    11101 tgcagcctgt gcaggctgtg tcgcgcacgg tgcggcagct gacgtactgg cggcgcgttt
    11161 tggaacgcgc gggatgctgg caaccgatct cttttccacg ctacagcgta ttgttaaccc
    11221 ggaagtgact gataaaaacc atgatgaatc gagtaattcc gctccctgat gagcaggcaa
    11281 cattagacct gggcgagcgg gtagcgaaag cctgcgatgg cgcaaccgta atctatctgt
    11341 atggcgattt aggcgcaggt aaaaccacct ttagccgggg ctttttacag gctctgggtc
    11401 atcagggtaa tgtcaaaagc cccacttata cgctggtcga accctatacg ctcgacaact
    11461 taatggtcta tcactttgat ttgtaccgcc ttgccgatcc cgaggagctg gagtttatgg
    11521 ggatccgcga ttattttgcc aacgatgcca tctgcctggt ggagtggcca caacaaggta
    11581 caggtgttct tcctgacccg gatgtcgaaa tacacattga ttatcaggca caaggccgtg
    11641 aggcgcgcgt gagtgcggtt tcctctgcgg gtgaattgtt gctggcgcgt ttagccggtt
    11701 aacctttgaa aggtggcggg atgatgtatc gcatcagaaa ttggttggta gcgacgctgc
    11761 tgctgctgtg cacgccggtg ggtgccgcga cgctctctga tattcaggtt tctaacggta
    11821 atcaacaggc gcggataacg ttgagtttta ttggcgatcc tgattatgcg tttagccatc
    11881 aaagcaaacg caccgtggcg ctcgatatca aacaaacggg catgattcag ggactgccgt
    11941 tgttgttcag cggcaataat ctggtgaagg cgattcgctc tggaacgcct aaagatgcac
    12001 aaacgctacg gctggtggtc gatcttaccg aaaacggtaa aaccgaagcg gtgaagcggc
    12061 agaatggcag caattacact gtcgtcttta cgattaacgc cgatgtgccg ccaccgcctc
    12121 ctccgccgcc cgtggttgcg aaacgcgttg aaacgcctgc ggttgtcgca ccgcgcgtca
    12181 gcgaaccggc gcgcaatccg tttaaaacgg aaagtaaccg cactacgggt gttatcagca
    12241 gtaatacggt aacgcgtccg gcagcgcgcg cgacggctaa cactggcgat aaaattatca
    12301 tcgctattga tgccggacac ggcggtcagg accctggcgc tatcggcccc ggtggtacgc
    12361 gggagaaaaa tgtcaccatc gccatcgcgc gtaaattgcg tactttgctc aatgacgatc
    12421 cgatgtttaa aggcgtttta acccgtgacg gggattactt tatctcggtg atggggcgca
    12481 gtgatgtggc acataagcaa aacgccaatt tcctcgtgtc gattcacgct gatgccgcac
    12541 cgaaccgtag tgcgactggc gcttccgtat gggtgctctc taaccgtcgc gccaacagtg
    12601 aaatggccag ctggctggag cagcacgaga aactgtcgga gctgctgggt ggggcgggtg
    12661 atgtgctggc gaacagtcag tctgacccct atttaagcca ggcggtgctg gatttacagt
    12721 tcggtcattc ccagcgggta gggtatgatg tagcgaccag tatgatcagt cagttgcaac
    12781 gcattggcga aatacataaa cgtcgaccag aacacgccag ccttggcgtt ctgcgctcgc
    12841 cggatatccc atcagtactg gtcgaaaccg gttttatcag caacaacagc gaagaacgtt
    12901 tgctggcgag cgacgattac caacaacagc tggcagaagc catttacaaa ggcctgcgca
    12961 attacttcct tgcgcatccg atgcaatctg cgccgcaggg tgcaacggca caaactgcca
    13021 gtacggtgac gacgccagat cgcacgctgc caaactaagg acgattgatg ccaattcagg
    13081 tcttaccgcc acaactggcg aaccagattg ccgcaggtga ggtggtcgag cgacctgcgt
    13141 cggtagtcaa agaactagtg gaaaacagcc tcgatgcagg tgcgacgcgt atcgatattg
    13201 atatcgaacg cggtggggcg aaacttatcc gcattcgtga taacggctgc ggtatcaaaa
    13261 aagatgagct ggcgctggcg ctggctcgtc atgccaccag taaaatcgcc tctctggacg
    13321 atctcgaagc cattatcagc ctgggctttc gcggtgaggc gctggcgagt atcagttcgg
    13381 tttcccgcct gacgctcact tcacgcaccg cagaacagca ggaagcctgg caggcctatg
    13441 ccgaaggacg cgacatggac gtgacggtta aaccggcagc gcatccggtg ggaacgacgc
    13501 tggaggtgct ggatctgttc tacaacaccc cggcgcggcg caaattcctg cgcaccgaga
    13561 aaaccgaatt tagccatatt gatgaaatca tccgccgcat tgcgctggcg cgtttcgacg
    13621 tcacgatcaa cctgtcgcat aacggtaaaa ttgtgcgtca gtaccgcgca gtgccggaag
    13681 gcgggcaaaa agaacggcgc ttaggcgcga tttgcggcac cgcttttctc gaacaagcgc
    13741 tggcgattga atggcaacac ggcgatctca ccctgcgcgg ctgggtggcc gatccaaatc
    13801 acactacgcc cgcactggcg gaaattcagt attgctacgt gaatggtcgc atgatgcgcg
    13861 atcgcctgat caatcatgcg atccgccagg cctgcgaaga caaactgggg gccgatcagc
    13921 aaccggcatt tgtgttgtat ctggagatcg acccgcatca ggtggacgtc aacgtacacc
    13981 ccgccaaaca cgaagtgcgt ttccatcagt cgcgtctggt gcatgatttt atctatcagg
    14041 gcgtgctgag cgtgctacaa cagcaactgg aaacgccgct accgctggac gatgaaccac
    14101 aacccgcacc gcgtgccatc ccggaaaacc gcgtggcggc ggggcgcaat cactttgcag
    14161 aaccggcagc tcgtgagccg gtagctccgc gctactctcc tgcgcccgcc tcaggcagtc
    14221 gtccggctgc cccctggccg aatgcgcagc caggctacca gaaacagcaa ggtgaagtgt
    14281 atcgccagct tttgcaaacg cccgcgccga tgcaaaaacc aaaagcgccg gaaccgcagg
    14341 aacctgcact tgcggcgaac agtcagagtt ttggtcgggt actgactatc gtccattccg
    14401 actgtgcgtt gctggagcgc gatggcaaca tttcactttt atccttgcca gtggcagaac
    14461 gttggctgcg tcaggcacaa ttgacgccgg gtgaagcgcc cgtttgcgcc cagccgctgc
    14521 tgattccgtt gcggctaaaa gtttctggcg aagaaaaatc ggcattagaa aaagcgcagt
    14581 ctgccctagc ggaattgggt attgatttcc agtcagatgc acagcatgtg accatcaggg
    14641 ccgtgccttt acccttacgc caacaaaatt tacaaatctt gattcctgaa ctgataggct
    14701 acctggcgaa gcagtccgta ttcgaacctg gcaatattgc gcagtggatt gcacgaaatc
    14761 tgatgagcga acatgcgcag tggtcaatgg cacaggccat aaccctgctg gcggacgtgg
    14821 aacggttatg tccgcaactt gtgaaaacgc cgccgggtgg tctgttacaa tctgttgatt
    14881 tacatccggc gataaaagcc ctgaaagatg agtgatatca gtaaggcgag cctgcctaag
    14941 gcgatttttt tgatggggcc gacggcctcc ggtaaaacgg cgttagccat tgagctgcgt
    15001 aaaattttac cagtagagtt gataagcgtt gattctgtcc ttatttacaa agggatggat
    15061 atcgggacgg cgaagccgaa cgctgaagag ttactcgccg cgccgcaccg attgctggat
    15121 attcgcgatc cgtcgcaggc ttactcggct gctgattttc gccgcgatgc gctggcggaa
    15181 atggccgata tcaccgcggc ggggcggatc ccactgttag tgggcggtac gatgttgtat
    15241 ttcaaggcat tgctggaagg gttgtcgccg ctaccgtcgg cagacccgga agtgcgggcc
    15301 agaattgagc aacaggcggc agagcaaggt tgggagtcat tgcatcgtca acttcaggag
    15361 gtagatccgg ttgcggcagc aaggattcat ccaaatgatc cacaaaggct ttcccgggca
    15421 ctggaagttt ttttcatttc gggtaaaact ttaacggaac tgacgcaaac gtcaggagac
    15481 gctctaccgt atcaggtgca tcagttcgcc atcgccccgg cgagccgtga actgctccat
    15541 caacgcattg agcagcgttt tcatcagatg ttggcttcag gttttgaagc agaagtccgg
    15601 gcgctttttg cccgaggaga tttgcatacg gacttgcctt ccattcgttg cgtgggttat
    15661 cgccagatgt ggtcttacct tgaaggcgaa atctcatacg atgaaatggt ttatcgaggt
    15721 gtttgcgcca cgagacagtt ggcgaagcgg cagataacct ggctgcgtgg ttgggaaggg
    15781 gttcactggc ttgacagtga aaaaccagaa caggcgcgtg acgaagtatt acaggttgtt
    15841 ggtgctatcg caggctgaat gtgtacaatt gagacgtatc gtgcgcaatt ttttcagaat
    15901 cgaaaggttc aaagtacaaa taagcatata aggaaaagag agaatggcta aggggcaatc
    15961 tttacaagat ccgttcctga acgcactgcg tcgggaacgt gttccagttt ctatttattt
    16021 ggtgaatggt attaagctgc aagggcaaat cgagtctttt gatcagttcg tgatcctgtt
    16081 gaaaaacacg gtcagccaga tggtttacaa gcacgcgatt tctactgttg tcccatctcg
    16141 cccggtttct catcacagta acaacgccgg tggcggtacc agcagtaact accatcatgg
    16201 tagcagcgcg cagaatactt ccgcgcaaca ggacagcgaa gaaaccgaat aaggtttcgg
    16261 gctgtttttt tacacgggga gccagcgatc ctgcgttccc cgctgatcta tttagagggt
    16321 tatacgcttg tttgaccgtt atgatgctgg tgagcaggcg gtactggtac acatctattt
    16381 tacgcaagac aaagatatgg aagacctcca ggagtttgaa tctctggtct cttccgccgg
    16441 tgtcgaagca ttgcaggtga ttaccggtag ccgtaaagcg ccgcacccaa agtattttgt
    16501 aggtgaaggt aaagcagttg aaattgcgga agctgtcaaa gcgatgggtg cttcggtcgt
    16561 tctttttgac catgccctga gcccggcgca agagcgtaac ctggagcgtt tgtgcgagtg
    16621 tcgtgttatc gaccgcaccg gccttatttt agatattttc gcccaacgtg cgcgtaccca
    16681 tgagggtaag ttgcaggttg agctggcgca gctgcgccat ctggctacgc gcctggtgcg
    16741 tggctggacc caccttgaaa gacagaaagg cgggataggt ttgcgtggtc cgggtgaaac
    16801 ccagctcgaa accgaccgtc gtttgttgcg taatcgcatc gtgcagatac agtcgcgcct
    16861 ggaaagagtt gaaaagcagc gtgagcaggg gcggcaatcg cgtatcaaag ccgacgttcc
    16921 tactgtttcg ctggtgggat ataccaacgc cggtaaatct acccttttca atcgcatcac
    16981 cgaagcgcgg gtctacgcgg cagaccagtt gtttgccacc ctcgacccga cgttgcggcg
    17041 tattgacgtt gcagatgtcg gtgaaaccgt acttgcagat accgtagggt ttattcgcca
    17101 cctgccgcac gatctggtgg cggcatttaa agccacgtta caagagacgc ggcaagccac
    17161 attactgctg cacgtcattg atgcggcgga tgtgcgtgta caagaaaaca tcgaagcggt
    17221 gaatacggtt cttgaagaga tcgacgctca cgagatccca accctgctgg tgatgaacaa
    17281 gatcgatatg ctggaagatt tcgaaccgcg tattgatcgg gacgaagaga acaaaccgat
    17341 ccgtgtctgg ctttccgcac agaccggagc ggggatacca cagctttttc aggctttgac
    17401 ggagcggctt tccggcgagg tggcgcagca tacattgcgt ctgccaccgc aggaagggcg
    17461 tctgaggagt cgtttttatc agcttcaggc aatagaaaaa gagtggatgg aggaggacgg
    17521 cagcgtaagt ctgcaagttc gtatgccgat cgttgactgg cgtcgcctct gtaaacaaga
    17581 accggcgttg atcgattacc tgatctaacg gcgtagcgtc tgaagcgtgg agtcatatcc
    17641 tctggcgtcg aaagacaaca gggatcaccg cataacaaat atggagcaca aacatggcgt
    17701 ggaatcagcc cggtaataac ggacaagacc gcgacccgtg gggaagcagc aaacctggcg
    17761 gcaactctga gggaaatgga aacaaaggcg gtcgcgatca agggccacct gatttagatg
    17821 atatcttccg caaactgagc aaaaagctcg gtggtctggg cggcggtaaa ggcaccggat
    17881 ctggcggtgg cagttcatcg caaggcccgc gcccgcagct tggcggtcgt gtcgttacca
    17941 tcgcagcggc agcgattgtc attatctggg cggccagtgg tttctatacc attaaagaag
    18001 ccgaacgcgg cgtggtaaca cgctttggta aattcagcca tctggttgag ccgggtctga
    18061 actggaaacc gacgtttatc gacgaagtca aaccggtgaa cgtggaagcc gtgcgtgaac
    18121 tggccgcttc tggtgtgatg ctgacgtcgg acgagaacgt agtgcgcgtt gagatgaacg
    18181 tgcagtaccg cgtcaccaat ccggaaaaat atctgtatag cgtgaccagc ccggatgaca
    18241 gcctgcgtca ggctaccgac agcgccctgc gtggagttat cggtaaatac accatggacc
    18301 gcattctgac ggaaggtcgt accgtgattc gtagcgatac tcagcgcgaa ctggaagaga
    18361 cgattcgtcc gtatgacatg ggtatcacgc tgctggacgt caacttccag gctgctcgtc
    18421 cgccggaaga agtaaaagcg gcgtttgacg atgcgattgc cgcgcgtgaa aacgaacagc
    18481 aatacattcg tgaagcagaa gcgtattcca acgaagttca gccgcgtgcg aacggtcagg
    18541 cgcaacgtat cctcgaagag gcgcgtgcgt acaaggccca gaccatcctg gaagctcagg
    18601 gtgaagtggc gcgctttgct aaacttctgc cggaatataa agccgcgccg gaaattactc
    18661 gcgagcgtct gtatatcgag acgatggaaa aagtgttggg taacacccgc aaagtgctgg
    18721 ttaacgataa aggtggcaac ctgatggttc tgccgttaga ccagatgctg aaaggtggta
    18781 acgcccctgc ggcgaagagc gataacggtg ccagcaatct gctgcgtctg ccgccagcct
    18841 cttcctccac aaccagtgga gcaagcaaca cgtcgtccac cagtcagggc gatattatgg
    18901 accaacgccg cgccaacgcg cagcgtaacg actaccagcg tcagggggaa taacgatgcg
    18961 taagtcagtt atcgcgatta tcatcatcgt gctggtagtg ctttacatgt ctgtctttgt
    19021 cgtcaaagaa ggtgagcgcg gtattacgct gcgttttggt aaggtactgc gtgacgatga
    19081 caacaaacct ttggtttatg agcctggtct gcatttcaag ataccgttca ttgaaacggt
    19141 gaaaatgctc gacgcacgta ttcagaccat ggacaaccag gccgaccgct ttgtgaccaa
    19201 agagaagaaa gacctgatcg tcgactctta catcaaatgg cgcatcagcg atttcagccg
    19261 ttactacctg gcaacgggtg gtggcgacat ttcgcaagcg gaagtgctgt tgaaacgtaa
    19321 gttctctgac cgtctgcgtt ctgaaattgg tcgcctggac gtgaaagata tcgtcaccga
    19381 ttcccgtggt cgtctgaccc tcgaagtacg tgacgcgctg aactccggtt ctgcgggtac
    19441 agaagatgaa gttactaccc cggcagcaga taacgccatt gccgaagcgg cagagcgcgt
    19501 aacggctgag acgaagggca aagttccggt catcaacccg aacagtatgg cggcgctggg
    19561 tattgaagtt gtcgatgtgc gtatcaagca gatcaacctg ccgaccgaag tgtctgaagc
    19621 gatctacaac cgtatgcgcg ccgagcgtga agcggtagcg cgtcgtcacc gttcacaagg
    19681 tcaggaagaa gcggaaaaac tgcgcgcgac tgccgactat gaagtgacca gaacgctggc
    19741 agaagctgag cgtcagggcc gcattatgcg tggtgaaggc gatgccgaag cagccaaact
    19801 gtttgctgat gcattcagta aagatccgga cttctacgca ttcatccgta gcctgcgtgc
    19861 ttatgagaac agcttctctg gcaatcagga cgtgatggtc atgagcccgg atagcgattt
    19921 cttccgctac atgaagacgc cgacttccgc aacgcgttaa tataacgact gcggtacagg
    19981 tcaataaagc caccgcatcc tcagggatgt cggtggtttt ctttttctat aaggataatg
    20041 aatgaattcg acaatctggc tggcgcttgc cctggttttg gtactggaag gtttagggcc
    20101 gatgctttac ccgaaggcat ggaagaagat gatctctgcg atgaccaatt tgcccgataa
    20161 tattttacgt cgttttggcg gtggacttgt ggttgcgggc gttgtggtct actacatgtt
    20221 gaggaaaacg attggctgaa caaaaaacag actgatcgag gtcatttttg agtgcaaaaa
    20281 gtgctgtaac tctgaaaaag cgatggtaga atccattttt aagcaaacgg tgattttgaa
    20341 aaatgggtaa caacgtcgtc gtactgggca cccaatgggg tgacgaaggt aaaggtaaga
    20401 tcgtcgatct tctgactgaa cgggctaaat atgttgtacg ctaccagggc ggtcacaacg
    20461 caggccatac tctcgtaatc aacggtgaaa aaaccgttct ccatcttatt ccatcaggta
    20521 ttctccgcga gaatgtaacc agcatcatcg gtaacggtgt tgtgctgtct ccggccgcgc
    20581 tgatgaaaga gatgaaagaa ctggaagacc gtggcatccc cgttcgtgag cgtctgctgc
    20641 tgtctgaagc atgtccgctg atccttgatt atcacgttgc tctggataac gcgcgtgaga
    20701 aagcgcgtgg cgcgaaagcg atcggcacca ccggtcgtgg tatcgggcct gcttatgaag
    20761 ataaagtggc acgtcgcggt ctgcgtgttg gcgacctttt cgacaaagaa accttcgctg
    20821 aaaaactgaa agaagtgatg gaatatcaca acttccagtt ggttaactac tacaaagctg
    20881 aagcggttga ttaccagaaa gttctggatg atacgatggc tgttgccgac atcctgactt
    20941 ctatggtggt tgacgtttct gacctgctcg accaggcgcg tcagcgtggc gatttcgtca
    21001 tgttcgaagg tgcgcagggt acgctgctgg atatcgacca cggtacttat ccgtacgtaa
    21061 cttcttccaa caccactgct ggtggcgtgg cgaccggttc cggcctgggc ccgcgttatg
    21121 ttgattacgt tctgggtatc ctcaaagctt actcaactcg tgtaggtgca ggtcctttcc
    21181 cggccgaact gtttgatgaa actggcgagt tcctctgcaa gcagggtaac gaattcggtg
    21241 caactacggg tcgtcgtcgt cgtaccggct ggctggacac cgttgccgtt cgtcgtgcgg
    21301 tacagctgaa ctccctgtct ggcttctgcc tgactaaact ggacgttctg gatggcctga
    21361 aagaggttaa actctgcgtg gcttaccgta tgccggatgg tcgcgaagtg actaccactc
    21421 cgctggcagc tgacgactgg aaaggtgtag agccgattta cgaaaccatg ccgggctggt
    21481 ctgaatccac tttcggcgtg aaagatcgta gcggcctgcc gcaggcggcg ctgaactaca
    21541 tcaagcgtat tgaagagctg accggtgtgc cgatcgatat catctctacc ggtccggatc
    21601 gtactgaaac catgattctg cgcgacccgt tcgacgcgta attctggtac gcctggcaga
    21661 tattttgcct gccgggctaa cagtgtgata cattgctgtg tcggttaagc cattacgcta
    21721 tccgacacag tgttaaatcc tcgctttttt ccttccccga actgaaataa attagcgaca
    21781 cagcttgtgg ctggtttatc atcaatataa atgtattttt tcccgatttc ccttttgagg
    21841 ttgatgtgca gttaacgagt ttcactgatt acggattacg tgcgctgatc tacatggcgt
    21901 cattgccaga agggcggatg accagtattt ctgaagtgac tgacgtctac ggcgtctccc
    21961 gtaatcatat ggtcaaaata atcaatcaac ttagtcgtgc cggctacgtg actgctgttc
    22021 gtggaaaaaa tggcggcatt cgcctgggta aaccggcgag tgcgatacgt attggtgatg
    22081 tggtgcgcga gctggagccc ttatcgctgg tgaattgcag cagtgagttt tgccacatta
    22141 cacctgcctg tcggttgaaa caggcgcttt ctaaggccgt gcaaagtttt cttacggaac
    22201 tggataacta cacgcttgcc gatttggttg aagagaatca accgctttat aaattattgc
    22261 tggtggagtg acgaaaatct tcatcagaga tgacaacgga ggaaccgaga tgtcacaaga
    22321 tcctttccag gaacgcgaag ctgaaaaata cgcgaatccc atccctagtc gggaatttat
    22381 cctcgaacat ttaaccaaac gtgaaaaacc ggccagccgt gatgagctgg cggtagaact
    22441 gcacattgaa ggcgaagagc agcttgaagg cctgcgtcgc cgcctgcgcg cgatggagcg
    22501 cgatggtcaa ctggtcttca ctcgtcgtca gtgctatgcg ctgccggaac gcctcgacct
    22561 ggtgaaaggt accgttattg gtcaccgtga tggctacggc tttctgcggg ttgaagggcg
    22621 taaagatgat ttgtatctct ccagcgagca gatgaaaacc tgcattcatg gcgatcaggt
    22681 gctggcgcag ccgctgggtg ctgaccgtaa aggtcgtcgt gaagcgcgta ttgtccgcgt
    22741 actggtgcca aaaaccagcc agattgttgg tcgctacttt accgaagcgg gcgtcggctt
    22801 tgtggttcct gacgatagcc gtctgagctt cgatatctta atcccgcccg atcagatcat
    22861 gggcgcgcgg atgggctttg tggtcgtagt cgaactgact cagcgtccga ctcgccgcac
    22921 caaagcggtg ggtaaaatcg tcgaagtgct gggcgacaat atgggcaccg gcatggcggt
    22981 tgatatcgct ctgcgtaccc atgaaattcc gtatatctgg ccgcaggctg ttgagcaaca
    23041 ggttgcgggg ctgaaagaag aagtgccgga agaagcaaaa gcgggccgtg tcgatttgcg
    23101 cgatttaccg ctggtcacca ttgatggcga agacgcccgt gactttgacg atgcagttta
    23161 ctgcgagaaa aaacgcggcg gcggctggcg tttatgggtc gcgattgccg acgtcagcta
    23221 ctatgtgcgt ccgccaacgc cgctggacag agaagcgcgt aaccgtggca cgtcggtgta
    23281 cttcccttcg caggttatcc cgatgctgcc ggaagtgctc tctaacggcc tgtgttcgct
    23341 caacccgcag gtagaccgcc tgtgtatggt gtgcgagatg acggtttcgt cgaaaggccg
    23401 cctgacgggc tacaaattct acgaagcggt gatgagctct cacgcgcgtc tgacctacac
    23461 caaagtctgg catattctgc agggcgatca ggatctgcgc gagcagtacg ccccgctggt
    23521 taagcatctc gaagagttgc ataacctcta taaagtgctg gataaagccc gtgaagaacg
    23581 cggtgggatc tcatttgaga gcgaagaagc gaagttcatt ttcaacgctg aacgccgtat
    23641 tgaacgtatc gaacagaccc agcgtaacga cgcgcacaaa ttaattgaag agtgcatgat
    23701 tctggcgaat atctcggcgg cgcgtttcgt tgagaaagcg aaagaaccgg cactgttccg
    23761 tattcacgac aagccgagca ccgaagcgat tacctctttc cgttcagtgc tggcggagct
    23821 ggggctggag ctgccgggtg gtaacaagcc ggaaccgcgt gactacgcgg aactgctgga
    23881 gtcggttgcc gaccgtcctg atgcagaaat gctgcaaacc atgctgctac gctcgatgaa
    23941 acaggcgatt tacgatccag aaaaccgtgg tcacttcggt ctggcattgc agtcctatgc
    24001 gcactttact tcgccgattc gtcgttatcc tgacctgacg ctgcaccgcg ccattaaata
    24061 tctgctggcg aaagagcagg ggcatcaggg caacaccact gaaaccggcg gctaccatta
    24121 ttcgatggaa gagatgttgc aactgggtca gcactgttcg atggcggaac gtcgtgccga
    24181 cgaagcaacg cgcgatgtcg ctgactggct gaagtgtgac ttcatgctcg accaggtagg
    24241 taacgtcttt aaaggcgtaa tttccagcgt cactggcttt ggcttcttcg tccgtctgga
    24301 cgacttgttc attgatggtc tggtccatgt ctcttcgctg gacaatgact actatcgctt
    24361 tgaccaggta gggcaacgcc tgatggggga atccagcggc cagacttatc gcctgggcga
    24421 tcgcgtggaa gttcgcgtcg aagcggttaa tatggacgag cgcaaaatcg actttagtct
    24481 gatctccagt gaacgcgcac cgcgcaacgt cggtaaaacg gcgcgcgaga aagcgaaaaa
    24541 aggcgatgca ggcaaaaaag gcggcaagcg ttgtcaggtc ggtaaaaagg taaactttga
    24601 gccagacagc gccttccgcg gtgagaaaaa aacgaagccg aaagcggcga agaaagacgc
    24661 gagaaaagcg aaaaagccat cggcgaaaac gcagaaaata gccgcagcga ccaaagcgaa
    24721 gcgtgcggcg aagaaaaaag tggcagagtg atcaataccc tctttaaaag aagagggtta
    24781 gattgctgac aaaatgcgct ttgttcatgc cggatgcggc gtgaacgcct tatccggcct
    24841 acataatcac gcaaattcaa tatattgcag agatcatgta ggccggataa acgtagtgca
    24901 tcaggcaaaa cgtaaacaac gagtacatta atgagcgaaa tgatttacgg catccacgca
    24961 gtgcaggccc tgctggagcg cgcccctgaa cgttttcagg aagtctttat tttaaaaggc
    25021 cgtgaagata aacgtctgtt accgctgatt cacgcccttg agtctcaggg cgtggttatc
    25081 cagttggcaa accgccaata tctcgacgag aaaagcgacg gtgccgtgca tcagggcatt
    25141 atcgcccgcg tgaagccagg acgtcagtat caggaaaacg atctgccgga tctgatcgct
    25201 tcgctcgatc aaccgttcct gctgatcctc gacggcgtaa ccgatccgca caacctcggc
    25261 gcgtgcctgc gtagtgcgga cgccgcaggc gttcatgcgg tgattgtgcc gaaagatcgc
    25321 tccgcacagc tcaacgctac ggcgaaaaaa gtagcctgcg gcgcggcaga aagcgttccg
    25381 ctgattcggg tgactaacct tgcgcgcacc atgcgtatgt tgcaggaaga gaatatctgg
    25441 atcgtcggta cggcaggcga ggcggatcat acactctatc agagcaaaat gaccggacgc
    25501 ctggcgctgg tgatgggcgc ggaaggtgaa ggtatgcgtc gcctgacccg tgaacattgc
    25561 gatgagttga tcagcatccc aatggcagga agtgtttctt ccctgaacgt ttcggttgca
    25621 accggaattt gcttatttga agcggttcgt cagcgcagct agttcctctg aattatgaaa
    25681 agccatccag atttggatgg ttt
//
LOCUS       DAZXUL010000056        25659 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZXUL010000056 DAZXUL010000000
VERSION     DAZXUL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25659
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.056"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACH3L1_002289"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACH3L1_002289"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFT4077001.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="ACH3L1_002290"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="ACH3L1_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFT4077002.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="ACH3L1_002291"
     CDS             2630..3391
                     /locus_tag="ACH3L1_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFT4077003.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="ACH3L1_002292"
     CDS             complement(3442..4326)
                     /locus_tag="ACH3L1_002292"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFT4077004.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACH3L1_002293"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACH3L1_002293"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFT4077005.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="ACH3L1_002294"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="ACH3L1_002294"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFT4077006.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="ACH3L1_002295"
     CDS             6202..6474
                     /locus_tag="ACH3L1_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFT4077007.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="ACH3L1_002296"
     CDS             6844..7920
                     /locus_tag="ACH3L1_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFT4077008.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="ACH3L1_002297"
     CDS             7947..9326
                     /locus_tag="ACH3L1_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4077009.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVDAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="ACH3L1_002298"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="ACH3L1_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="ACH3L1_002299"
     CDS             10409..11245
                     /locus_tag="ACH3L1_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFT4077010.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="ACH3L1_002300"
     CDS             11250..12197
                     /locus_tag="ACH3L1_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFT4077011.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="ACH3L1_002301"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="ACH3L1_002301"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFT4077012.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="ACH3L1_002302"
     CDS             13324..14082
                     /locus_tag="ACH3L1_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFT4077013.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="ACH3L1_002303"
     CDS             14200..15558
                     /locus_tag="ACH3L1_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4077014.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACH3L1_002304"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACH3L1_002304"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFT4077015.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACH3L1_002305"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACH3L1_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFT4077016.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACH3L1_002306"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACH3L1_002306"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFT4077017.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="ACH3L1_002307"
     CDS             19503..20054
                     /locus_tag="ACH3L1_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFT4077018.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="ACH3L1_002308"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="ACH3L1_002308"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFT4077019.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="ACH3L1_002309"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="ACH3L1_002309"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFT4077020.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="ACH3L1_002310"
     CDS             23307..24347
                     /locus_tag="ACH3L1_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFT4077021.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACH3L1_002311"
                     /pseudo
     CDS             complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACH3L1_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094017.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
BASE COUNT         6518 a         5971 c         6623 g         6547 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcga tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg ttcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatatgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt attcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagta
//
LOCUS       DAZXUL010000057        24854 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZXUL010000057 DAZXUL010000000
VERSION     DAZXUL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24854
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.057"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..246
                     /locus_tag="ACH3L1_002312"
     CDS             <1..246
                     /locus_tag="ACH3L1_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFT4077022.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACH3L1_002313"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACH3L1_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFT4077023.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACH3L1_002314"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACH3L1_002314"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFT4077024.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="ACH3L1_002315"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="ACH3L1_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFT4077025.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACH3L1_002316"
     CDS             complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACH3L1_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFT4077026.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACH3L1_002317"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACH3L1_002317"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFT4077027.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="ACH3L1_002318"
     CDS             complement(4442..5650)
                     /locus_tag="ACH3L1_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4077028.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="ACH3L1_002319"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="ACH3L1_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFT4077029.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="ACH3L1_002320"
     CDS             complement(6445..8130)
                     /locus_tag="ACH3L1_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFT4077030.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="ACH3L1_002321"
     CDS             8400..8777
                     /locus_tag="ACH3L1_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFT4077031.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACH3L1_002322"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACH3L1_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFT4077032.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="ACH3L1_002323"
     CDS             9224..9511
                     /locus_tag="ACH3L1_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFT4077033.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="ACH3L1_002324"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="ACH3L1_002324"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFT4077034.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="ACH3L1_002325"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="ACH3L1_002325"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFT4077035.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="ACH3L1_002326"
     CDS             11268..11744
                     /locus_tag="ACH3L1_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFT4077036.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="ACH3L1_002327"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="ACH3L1_002327"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFT4077037.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14436
                     /gene="potG"
                     /locus_tag="ACH3L1_002328"
     CDS             13303..14436
                     /gene="potG"
                     /locus_tag="ACH3L1_002328"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFT4077038.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14446..15399
                     /gene="potH"
                     /locus_tag="ACH3L1_002329"
     CDS             14446..15399
                     /gene="potH"
                     /locus_tag="ACH3L1_002329"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFT4077039.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15396..16241
                     /gene="potI"
                     /locus_tag="ACH3L1_002330"
     CDS             15396..16241
                     /gene="potI"
                     /locus_tag="ACH3L1_002330"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFT4077040.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16301..16789
                     /locus_tag="ACH3L1_002331"
     CDS             16301..16789
                     /locus_tag="ACH3L1_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFT4077041.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16830..17957
                     /gene="rlmC"
                     /locus_tag="ACH3L1_002332"
     CDS             16830..17957
                     /gene="rlmC"
                     /locus_tag="ACH3L1_002332"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFT4077042.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="ACH3L1_002333"
     CDS             complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="ACH3L1_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFT4077043.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19155..19823)
                     /gene="artM"
                     /locus_tag="ACH3L1_002334"
     CDS             complement(19155..19823)
                     /gene="artM"
                     /locus_tag="ACH3L1_002334"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFT4077044.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="ACH3L1_002335"
     CDS             complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="ACH3L1_002335"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFT4077045.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALELSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20546..21277)
                     /gene="artI"
                     /locus_tag="ACH3L1_002336"
     CDS             complement(20546..21277)
                     /gene="artI"
                     /locus_tag="ACH3L1_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFT4077046.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21295..22023)
                     /gene="artP"
                     /locus_tag="ACH3L1_002337"
     CDS             complement(21295..22023)
                     /gene="artP"
                     /locus_tag="ACH3L1_002337"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFT4077047.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22241..22756)
                     /locus_tag="ACH3L1_002338"
     CDS             complement(22241..22756)
                     /locus_tag="ACH3L1_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFT4077048.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23405..>24854
                     /locus_tag="ACH3L1_002339"
     CDS             23405..>24854
                     /locus_tag="ACH3L1_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFT4077049.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSL"
BASE COUNT         6266 a         5959 c         6299 g         6330 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc gccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg tatgctggca
    13501 ggtttcgaac aaccttctgc cggacagata atgcttgatg gcgtcgattt gtcacaggtt
    13561 ccgccttacc tgcgccccat caatatgatg tttcagtctt acgcgctgtt tccgcatatg
    13621 accgtggaac agaacatcgc ttttggcctg aaacaggaca aactaccgaa agcggaaatt
    13681 tccagtcggg tcaatgagat gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa
    13741 ccgcatcagc tttctggtgg tcagcgacag cgtgtggccc tggcccgaag ccttgccaag
    13801 cgcccgaaac tattactgct cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac
    13861 cggatgcagc ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc
    13921 acccacgatc aggaagaggc gatgaccatg gcggggcgca tcgccattat gaatcgtggg
    13981 aaatttgtcc agattggcga accggaagag atctacgagc atccgactac ccgctatagc
    14041 gccgaattta ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat
    14101 ggcttggtgc ttgattcgcc ggggctggtg catccactga aagtcgacgc ggatgcctcg
    14161 gtggtcgata acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa
    14221 gagccgcccg ccaatggttg taacttcgcg gtgggggagg tgatacacat tgcctatctc
    14281 ggcgatcttt cggtgtatca cgttcgtctg aaaagtgggc agatgattag tgcccagcta
    14341 caaaacgccc atcgtcatcg taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc
    14401 tgggaagtgg acagctgtgt ggtgctgacg gtttaaggag caaagatgaa tacacttgaa
    14461 cctgctgccc agtcgaaacc gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg
    14521 aagcatgggc gcaaactggt cattgcgttg ccatatatct ggttgatctt gctgtttctg
    14581 ctgccatttc tgattgtctt taaaataagc ctggcggaga tggcgcgcgc tattccacct
    14641 tataccgaac tgatggagtg ggctgacggg caactgtcca tcactcttaa tctcggtaat
    14701 tttctgcaac tgaccgacga tccgctctat ttcgatgctt atctccagtc gctacaggtg
    14761 gctgcgattt cgacattttg ctgtttactg atcggctatc cgctggcgtg ggcggtggcg
    14821 cacagtaagc cttcgacccg taatatttta ttactactgg tgatcctgcc gtcgtggacc
    14881 tcgtttctga tccgcgttta tgcttggatg ggaatattaa aaaacaacgg tgtgctgaat
    14941 aattttctgc tgtggctggg ggttatcgat caaccgctga ccattcttca taccaatctg
    15001 gcagtttata ttggcattgt ttacgcctac gtgccgttta tggtgctgcc gatttatacc
    15061 gcgttgattc gtattgatta ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg
    15121 ctgaaaacgt tctttactgt gatcgtgccg ctgactaaag gtgggattat tgccggatcg
    15181 atgctggtgt ttatcccggc tgtgggcgag tttgtgatcc cggaactgct cggtggcccg
    15241 gacagcatca tgatcgggcg cgtgctatgg caagagtttt tcaataaccg cgactggccg
    15301 gtggcctcgg cggtagcgat catcatgttg ctgctgctaa ttgtgccgat aatgtggttc
    15361 cacaaacacc agcaaaaaag cgtgggagaa cacggatgaa taatttaccg gtagttcgtt
    15421 cgccctggcg gattgtgatt ttgctgctgg gctttacctt tctctacgcg ccaatgctga
    15481 tgctggttat ctattcgttt aacagctcga agctggtgac ggtgtggggc ggctggtcaa
    15541 cgcgctggta tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc
    15601 tgacaattgc tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt gcggcggtgg
    15661 tgctggtgcg ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc
    15721 cgctagtaat gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg
    15781 ctcatgccat tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca
    15841 ctttctgtac ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct
    15901 cgatagaaga agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta
    15961 cgctaccgat gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc
    16021 ttgatgatct ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc
    16081 tggtcttttc cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga
    16141 tcctcggcgc ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa
    16201 aacagcggat acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa
    16261 cctggctaca taatgccgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg
    16321 attttttaag aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt
    16381 ggcggcgctc gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct
    16441 gggcgtgcgc ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct
    16501 ggtcttttta agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt
    16561 gaaagggcgt cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta
    16621 cttgtgggcg gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc
    16681 aaaacgtgaa atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat
    16741 gctgctgttc gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc
    16801 gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct
    16861 gtcgttcctg tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg
    16921 atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc
    16981 cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac
    17041 tgctcggtat gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc
    17101 cagcctcatt tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa
    17161 cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc
    17221 agagtgatgg cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac
    17281 tgcgtaaggc gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca
    17341 atattcagcc ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac
    17401 aacaggcact ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct
    17461 tccagactaa tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac
    17521 agctgccggt taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact
    17581 gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg
    17641 caaagcagtc agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca
    17701 ctcagtttgc cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc
    17761 gcggcattgg taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct
    17821 actccagctg taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca
    17881 tcgaacgggt acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc
    17941 tgctggtgaa gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt
    18001 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat
    18061 acattgcaga gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc
    18121 atcagtctca attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct
    18181 ttaattgctg ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca
    18241 gcgataccca ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc
    18301 agttgcggat tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca
    18361 atacgaccat ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc
    18421 acttccgggt gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg
    18481 cgtttgcctt tcaggtcggc aaacgttttg taggtatctt ttttggaaat cacgacggct
    18541 gagttttcat agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc
    18601 ataccggaga ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac
    18661 gcgtgattag taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg
    18721 atatcaaagc cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct
    18781 gaaacgccaa aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa
    18841 gcggcaagaa ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc
    18901 cgtgtctgcg gcatgggata acaatgccat caagtgaatt tatatgcaat aaacatgatt
    18961 aaataattta aatgaaataa aaaaagacgg ataacttagt gagttgtccg tcttcattat
    19021 aagaatttat gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc
    19081 gtgctgatgt ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa
    19141 tgtacgcagc tacattaatt ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc
    19201 atcatcagcg tcagcaggcc gttgacgacc aagtaaataa tccctgccgc accgaacacc
    19261 attacatcgt aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta
    19321 atggtgtaag ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag
    19381 agcgagcgtt taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc
    19441 agggcgctac aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc
    19501 tgcgtggtat acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct
    19561 gacaacaaat gccacagtgc cggatactcc tgcaacgtcg gaaactggcc cggcccgtaa
    19621 taaatcagga agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc
    19681 accagccaca ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga
    19741 atcagtgcca caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc
    19801 agctcgggta aatactcaaa cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt
    19861 caatgcgttt gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca
    19921 caatgtacca ggtaaatggt tcctgggtac gagtagcaat gcttttggtt tgtaacatta
    19981 aatcattcac actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt
    20041 taccgaggcc aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag
    20101 ccgatttcga cagctccagc gcctgaccag attcccactg gcccaccggt acagctttca
    20161 acgcgccgcg cagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac
    20221 cacaaaggaa cggactcacg tcgaagttct caatgtccat ctgcaccggg atctgcacga
    20281 acccaagatt gatagtgaag ccatccgaaa gcgtcagcag taactgcgag gagccaaaat
    20341 agataaacag cactaccaga atttctggca ggccacgcag aatggttacc agcgctgaac
    20401 ctgcccacgc gacaggacgc catttcgctg actcccatac cgcaaagaac atcgccagcg
    20461 ccagcccgac aatcaatgca caaacggcaa ggccgacggt catcccggcg gcgcttgcta
    20521 aaggaaaaaa ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt
    20581 aagtgccatc tttcttcact ttttccagcg cagtgttgag tttctgctgc agttcagtgt
    20641 ttccctgacg taccgcgatg ccgaggccag taccgaaata atctttatcg gtcactttat
    20701 cgcccactgc cgccagtttt gggttatctt tcagccactc agtgaccact gcggtgtcac
    20761 caaatacgct gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat
    20821 acggaacggt ggtgatttcc gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt
    20881 tctgaacgcc gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac
    20941 ccacaaacag ggcagagttg tcatagtacg gggtagtaaa cagtacctgc ttttcacgct
    21001 ccggagtgat atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga
    21061 tcaggctgtc aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg
    21121 cctgcgccag gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg
    21181 gaggataggg ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa
    21241 aacctgcaat taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg
    21301 agagagatag tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg
    21361 ttctacgata tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc
    21421 aacttcgtgg gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct
    21481 gacgatttgt gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac
    21541 ctgcggttcc atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag
    21601 atgcagcggg taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc
    21661 acgggccagc gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt
    21721 ttgctgcacg gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg
    21781 acgcaaatcg cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc
    21841 aatgttgagc gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct
    21901 tttacccgcg ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt
    21961 gatatcgaac agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact
    22021 cattgacact cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttaac
    22081 agaataatta tgcaatattt atgctttaaa agttaaaagc aaagcgcatt attcaataaa
    22141 catagcacaa aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa
    22201 atgccggata atttacgggg tttattggtt gatcaaggcg ttagcgattc tcgatggact
    22261 gacggagcgt acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga
    22321 cccaacactg gccttcctga atcattaaca cttcatcctg ccagccctgg tcaccctgtt
    22381 tgagatccac ccgcaatgga atgttacggg catcacgatt agggatagtc gatgcactgg
    22441 caacgtgggc gctgtctggc aaggtggttc gactggagaa cggatcgctg cttaacaatt
    22501 cacgatgact attatcgcgg ctggcatcgc taagttgggt tgccagtttg tcactcaaat
    22561 aggggcgcag ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg tagaattgtt
    22621 gagccacact gtcaggccct ccctggacac aggaaccggt gcgggggccg ttatctttat
    22681 aagctggcgt gacggtggta caggcactca gcagcagtgc acatggaata agtagagata
    22741 gtgaacggta gcgcataatc atttccttat tagcttgctg tctgcgtcac actacgacca
    22801 aacgctatca gattatttaa tacattgttt ttaatacaat tctgaaacaa tctgaacacc
    22861 tgattgggtt tgagtccatt gctggcgacc cactcaaggt tgctcgtagc tgacttactt
    22921 aaattaccca aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt tatatggctg
    22981 aaatgatcat ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc ctcctgtaga
    23041 ggttaagagc atctgcaaga agccagtttc acatttataa cgcgtgataa atgaatgcgg
    23101 ttattgtcta atcattacaa aatttttcac atgcgataag agatcctgcg attaagatga
    23161 ttgccaaaat tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc agctttgact
    23221 ctcgataata ttatttatgc tacaattgtg ggtaaagaat ttaagctaat gaatttgagt
    23281 gaagaataaa ttaaattgct tcagtatttc gttaattaat ataaatacaa aaggcgttgt
    23341 gcttttataa ttataaagcg cgaggagttc ggtggattat cgtttttctt gggtgatttt
    23401 taatatgata aacaatatat ctgatcaagc atcatcattt ccaggcacac aactaaatca
    23461 aagtgataat ttcctggatt cactgaggga atttttcgcg atattaaatc ccagtcgtaa
    23521 gggtgagttg tctacatggg ataccatcta cttgcatctg atattagcaa taaatgctga
    23581 cagtgatctc ataaaaaatg atgtgttgct cgcggaaaat attccatcag cgaattacca
    23641 atttaataca ttttttagca atactttcga gattgatgtt aaaaaatatc tgggtaaatc
    23701 agaagataac gaagttgaaa taaaagcggg taatgaaaga atctctattg gaatacgtaa
    23761 tgtatcgaat ggaaaacttg aaagacaaca gtttcttttc ccactggact atgagaataa
    23821 attacaagaa caattagata agtattttac gattgaatcg cacccattat tatacaggta
    23881 taccattggt agtaagattg cgaatgtgat ttttgaaaag ttatacagtc gtattgactt
    23941 taacaaagaa caatatatat cctttataaa ggacgcattt attcattttt atgattatag
    24001 caggcgatat gctattagtg agaatatcga taaagacgct gtaacaaaca atatcgcatt
    24061 gatgagcact ttttatgaca gtgataatac gtcaggtgag gttttaaata atgattttac
    24121 agaagaagag tcatttgaaa ctgcgttaga tgtagagcat gcaatagtgc ttggatttgc
    24181 tgatgataat tttgaaacaa aacctgttca ttatcaggat ttattaacac gatttagtgc
    24241 ctttcaagat accgtgttta atctgtttcc tgaaatgcac tcttcgcatt accacgatat
    24301 ttgtagcgta agtgtggata tgacaaaggg aacgcaatgc atgattcact tgatggtgaa
    24361 tgaagaagtt ttcatgtctc tgcctgttcc agttgccacg atggtacgtg aagatgcaag
    24421 taatttagtc aatcttaaaa cactactcaa tgatggttgt ttcattaaat atagtcattt
    24481 taatgacgtg gcattaataa agcaaaacat aagtaatctc tatttatcac acactgttgt
    24541 gaatgaaagc atattgaaaa aatgttgttt tgaaaacggc agtttaggtg atgttaaaat
    24601 aacaaatagc aatgtaataa acagtgcatt caaaaatatt tcgtttagat ctgttaaaat
    24661 taataacgta aatactcatt ctttaaaatt tattaactgc aattttttca atgttgatat
    24721 gataagagtt aatctcagca aatgtctttt tcatgaatgt agtatgcatg gtgtcaagat
    24781 aaaaccctgg ttgcctgtaa aatggacaaa agaattaata agtgattatt tgtatggctg
    24841 tttactatct ctgt
//
LOCUS       DAZXUL010000058        24800 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZXUL010000058 DAZXUL010000000
VERSION     DAZXUL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24800
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.058"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..386)
                     /locus_tag="ACH3L1_002340"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="ACH3L1_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="ACH3L1_002341"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="ACH3L1_002341"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFT4077050.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="ACH3L1_002342"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="ACH3L1_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFT4077051.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="ACH3L1_002343"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="ACH3L1_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFT4077052.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="ACH3L1_002344"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="ACH3L1_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFT4077053.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="ACH3L1_002345"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="ACH3L1_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFT4077054.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="ACH3L1_002346"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="ACH3L1_002346"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFT4077055.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="ACH3L1_002347"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="ACH3L1_002347"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFT4077056.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="ACH3L1_002348"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="ACH3L1_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFT4077057.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="ACH3L1_002349"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="ACH3L1_002349"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFT4077058.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="ACH3L1_002350"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="ACH3L1_002350"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFT4077059.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="ACH3L1_002351"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="ACH3L1_002351"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFT4077060.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="ACH3L1_002352"
     CDS             10748..11212
                     /locus_tag="ACH3L1_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFT4077061.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="ACH3L1_002353"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="ACH3L1_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFT4077062.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /gene="selO"
                     /locus_tag="ACH3L1_002354"
     CDS             12236..13672
                     /gene="selO"
                     /locus_tag="ACH3L1_002354"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFT4077063.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACH3L1_002355"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACH3L1_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFT4077064.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACH3L1_002356"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACH3L1_002356"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFT4077065.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACH3L1_002357"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACH3L1_002357"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFT4077066.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="ACH3L1_002358"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="ACH3L1_002358"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFT4077067.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACH3L1_002359"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACH3L1_002359"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFT4077068.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="ACH3L1_002360"
     CDS             complement(20505..20798)
                     /locus_tag="ACH3L1_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFT4077069.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCSNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="ACH3L1_002361"
     CDS             complement(20795..22084)
                     /locus_tag="ACH3L1_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFT4077070.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="ACH3L1_002362"
     CDS             complement(22140..23078)
                     /locus_tag="ACH3L1_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFT4077071.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="ACH3L1_002363"
     CDS             complement(23098..23862)
                     /locus_tag="ACH3L1_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFT4077072.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24182..24661
                     /locus_tag="ACH3L1_002364"
                     /pseudo
     CDS             24182..24661
                     /locus_tag="ACH3L1_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
     gene            24716..>24800
                     /locus_tag="ACH3L1_002365"
     CDS             24716..>24800
                     /locus_tag="ACH3L1_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFT4077073.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQ"
BASE COUNT         6133 a         5983 c         6523 g         6161 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc ggtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt actgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg ggggcgacgg agcaagctgt tttccttgcc aacaatcttg caaaaaaaat
    24001 gcgattgcta aaacaaaagt gcaatttgcg gcaatatagt tattccatga ccgtacaatc
    24061 ttgcattcag tgtaaataat cttgtttagc tctcgctaaa cgcggttgta taaccgcaat
    24121 acacgccgta ttttatccgg catatattgt ttttgcttgc tcatggaata gggaggttgt
    24181 catgtatcaa cgctgttttg ataatgccag tgaaacgctg tttgtcgccg gtaaaacgcc
    24241 acggctttca cgttttgcat ttagcgatga tccaaaatgg gagtctggac atcacgttca
    24301 tgacaatgaa accgagctga tttacgtcaa gaaaggggtt gcaaggttta ccatcgattc
    24361 ttcgttatat gtcgcacatg cggatgacat tgtggtgata gaacgcggca ggctgcatgc
    24421 ggtggcctct gacgttaacg atccggcaac gacgtgtacc tgtgcgctgt acggctttca
    24481 gtttcagggg gctgaggaaa atcagttact gcaaccgcat tcttgtccgg taattgccgc
    24541 ggggcaggga aaagaagtca taaaaacctt atttaatgag ctaagtgtga ttttgccgca
    24601 aagtaaaaat agccaaacat cttcgttatg ggatgcatta gcctatacat tagcaattct
    24661 ggtaatgctg ccaacttact gatttagtgt atgatggtga ttttaaggtg cttgcgtggc
    24721 ttccatttcc atcagatgtc cttcctgctc cgctactgaa ggcgtggtgc gtaacggcaa
    24781 aagcactgcc ggacatcagc
//
LOCUS       DAZXUL010000059        24186 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZXUL010000059 DAZXUL010000000
VERSION     DAZXUL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24186
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.059"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(25..597)
                     /locus_tag="ACH3L1_002366"
     CDS             complement(25..597)
                     /locus_tag="ACH3L1_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFT4077074.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="ACH3L1_002367"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="ACH3L1_002367"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFT4077075.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="ACH3L1_002368"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="ACH3L1_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFT4077076.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="ACH3L1_002369"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="ACH3L1_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFT4077077.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="ACH3L1_002370"
     CDS             complement(3559..5340)
                     /locus_tag="ACH3L1_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFT4077078.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="ACH3L1_002371"
     CDS             complement(5333..7105)
                     /locus_tag="ACH3L1_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFT4077079.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYNTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="ACH3L1_002372"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="ACH3L1_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFT4077080.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="ACH3L1_002373"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="ACH3L1_002373"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFT4077081.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10357
                     /locus_tag="ACH3L1_002374"
                     /pseudo
     CDS             8665..10357
                     /locus_tag="ACH3L1_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10422..11117
                     /gene="queC"
                     /locus_tag="ACH3L1_002375"
     CDS             10422..11117
                     /gene="queC"
                     /locus_tag="ACH3L1_002375"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFT4077082.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="ACH3L1_002376"
     CDS             complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="ACH3L1_002376"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFT4077083.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11661..12032)
                     /locus_tag="ACH3L1_002377"
     CDS             complement(11661..12032)
                     /locus_tag="ACH3L1_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFT4077084.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="ACH3L1_002378"
     CDS             complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="ACH3L1_002378"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFT4077085.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14246..14536)
                     /gene="hupB"
                     /locus_tag="ACH3L1_002379"
     CDS             complement(14246..14536)
                     /gene="hupB"
                     /locus_tag="ACH3L1_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFT4077086.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14727..17081)
                     /gene="lon"
                     /locus_tag="ACH3L1_002380"
     CDS             complement(14727..17081)
                     /gene="lon"
                     /locus_tag="ACH3L1_002380"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFT4077087.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="ACH3L1_002381"
     CDS             complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="ACH3L1_002381"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFT4077088.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="ACH3L1_002382"
     CDS             complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="ACH3L1_002382"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFT4077089.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19539..20837)
                     /gene="tig"
                     /locus_tag="ACH3L1_002383"
     CDS             complement(19539..20837)
                     /gene="tig"
                     /locus_tag="ACH3L1_002383"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFT4077090.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="ACH3L1_002384"
     CDS             complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="ACH3L1_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFT4077091.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21803..22381
                     /locus_tag="ACH3L1_002385"
     CDS             21803..22381
                     /locus_tag="ACH3L1_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFT4077092.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22425..23900
                     /gene="ampG"
                     /locus_tag="ACH3L1_002386"
     CDS             22425..23900
                     /gene="ampG"
                     /locus_tag="ACH3L1_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFT4077093.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6666 c         5828 g         6025 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacag taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtattg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatggcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa ctggccgaac gctgtttttg
     8761 cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaggcgggat ggctggagtg
     8821 gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca cgccggaatc
     8881 gctacgcaat gcgatgatgg aacaggcact ggaaaccgga aagcagcaag atgtgctgga
     8941 gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccattta tgggcggaca
     9001 atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg aaccgctaca
     9061 accaggcttt ttgcccggcc gtgccgagca gcatctcgcc gggcagatat tttccggcct
     9121 gacccgcttc gataataata ctcagcgccc gattggcgat ttagcgcatc actgggaaac
     9181 ctctactgac aggttacgct gggactttta tcttcgttca accctacact ggcataacgg
     9241 cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac aactgccagc
     9301 actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc agtgtctgac
     9361 cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct attgcagcca
     9421 tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa cacaattcac
     9481 agcggagctg gtgcgcctgg agagccatga ttattaccat ttgcgtcatc cgctgcttaa
     9541 agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa ccagttgtcg
     9601 gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca gccaagtcag
     9661 tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc gactctccct
     9721 ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac aaacgttaga
     9781 agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga ctattccgca
     9841 ctggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt atcacctacc
     9901 gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg aaggctgtga
     9961 actcacaatt atttttcata acgcaaaaaa ctgggacgac acgaccctac tggcacacgc
    10021 agacctcatg atgggcgaca gattaattgg cgaagcaccg gaatatactc tggagcaatg
    10081 gctacgttgc gatccactat ggccacatgt tttcgacgct ccagcatatg cacatctgca
    10141 atcgacgctg gacgcggtgc aagtaatgcc tgatgaggaa aaccgattta atgccctgaa
    10201 agcggttttt agccagttaa tggcagacgc gacgctgacg ccgctgttca actatcacta
    10261 tcgcattagt gcccctcccg gcgtgaacgg tgtgcgactg acaccgcgcg gctggtttga
    10321 atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata gctggtccgg gttaatcgca
    10381 ggcgttacca taacgctttt attgttcaag caggattatc tatgaaacgt gctgtcgttg
    10441 tgttcagtgg aggccaggat tccaccacct gtctggtgca ggcattacaa caatatgatg
    10501 aagtccattg cgtgacgttc gattacggtc agcgacatcg cgcagaaatc gacgtggcac
    10561 gcgaactggc gctgaaactg ggagcacgcg cgcataaggt tcttgatgta acgctgctca
    10621 acgagctggc ggtcagcagc ctgacacgtg acagcattcc ggtgcctgat tatgaacctg
    10681 aagccgatgg catcccgaat acgtttgtcc cagggcgtaa tattttgttc ctgacgctgg
    10741 cggcaatata tgcgtatcag gtaaaagcag aagccgtaat taccggcgtc tgtgaaacgg
    10801 atttctccgg ctacccggat tgccgcgatg agtttgtgaa agcactaaac catgccgtca
    10861 gtttgggcat ggcgaaagat attcgttttg aaacgccgct gatgtggatt gataaagcgg
    10921 aaacctgggc gctggcagat tattacggca aactggattt agtccgtaac gaaacgttga
    10981 cctgctataa cggcattaaa ggcgacggtt gcggtcattg tgcggcatgt aatttacgcg
    11041 ccaacggttt gaatcattat ctggccgata aaccgacggt gatggcagcg atgaagcaga
    11101 aaaccgggtt gaggtaatta ttccgggtgt cgccggatgc ggcttgagca tccggcacca
    11161 caaaacgttt acttaaccat ctgctccagc ttttcgcgca attccccttc cagagctaat
    11221 gctttctgcg ttttaagatc aatacaaaca aacgtaataa gcgcatccgc taccacctgc
    11281 ccttccggct ccagtgtaat gacctggttt aagatgccgc ttttaccgtt taattgctgc
    11341 aactgactgg taatagttaa caggtcgctt aataccgctg gacgacgata gttaatattg
    11401 atattgacca cgacgaaggc gatgttatgg gccgtcatcc actgaaaact gtcgctattt
    11461 tccaccccat cccagcgggc ttcttcgaga aattcaaggt agcgggcgtt gttgacgtgc
    11521 tggtaaacgt cgagatgata tccacgaact ttgatttgtg tttgcatagc gcaataacct
    11581 tacgttgttg ttataagtac agaggtcata actggtatga cctcttcagt ctggcaaaaa
    11641 attgccacta tgcaaattaa ttacagggtt aataccgcca gattacgttc caccagcgaa
    11701 ttgcccatcc ccggcacctg ctttagatcc tccaccgttt taaacggacc gtactcttcg
    11761 cgataactga caatcgcctg cgctttcttc aggccaacgc cattcatcgc gcgggctagc
    11821 tcttccgcgc tggcattatt aatgctgacc cgggtgcctt cttcgtcact ggctttcgcc
    11881 ggtactgctg ctttactttg tgctgcagga gcttccgctt tggtttctac cgccgtcggt
    11941 ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc cagggacagt
    12001 gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt gttaacagtg
    12061 gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa atggaaaagg
    12121 ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag gcggcttcag
    12181 gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg caggttactc
    12241 atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac catcgctttt
    12301 ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag aaccacatta
    12361 ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag tggcagtgca
    12421 aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg ctcgccaaat
    12481 ttcagaccgg cagcctgcat agcttccgca cctttgccgg ctttcaaatc aaccagcagt
    12541 ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag tgccttaact
    12601 tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct gacgcgcagc
    12661 acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccga cgcgccgttt
    12721 tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt caactcttcc
    12781 ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc agcttgctct
    12841 gcaccggcca gagactcggt gtcgttgctt gccgcatcgc tcactttctg ctgcagcgca
    12901 tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc acgtacttcg
    12961 tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag gaaaccgacc
    13021 gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt tttcagttcg
    13081 tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc agagataata
    13141 tcggcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag ctcatcaagt
    13201 accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt acgctgcggc
    13261 tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc gctaaccggt
    13321 tgctgcatcg ttgcggcaac cagcttgatg taactcacgc ggaattgttc cggcgtcatg
    13381 aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac aggctgcttc
    13441 gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg cgcgaccagt
    13501 gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac gccgttaatc
    13561 agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc ggtcatcccc
    13621 atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac ctggaaggct
    13681 ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt cagctcacga
    13741 gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg acgcagggtt
    13801 ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg ttgctgcata
    13861 cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc ctggtcattc
    13921 acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc ggtcaatatg
    13981 aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc agccgtgcgt
    14041 aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc tcacgcaacg
    14101 tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca gcccacaaga
    14161 cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc cttgaacttc
    14221 gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct ttacctgcac
    14281 ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc gggttgcggc
    14341 cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt gctacatcat
    14401 ccccttcttt cagagattca gttacggaag caataatagc atctaacgca cggccagccg
    14461 cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat ttattcactc
    14521 ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga acgcgcagca
    14581 gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc ccgatccgct
    14641 atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc agccctgttt
    14701 ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat gccagacggt
    14761 tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg aatgtccaga
    14821 tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa cggaattagc
    14881 actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc accgatcggc
    14941 agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg aaccgggtta
    15001 ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg accatctttc
    15061 ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa atcagggttg
    15121 atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat agactcctgc
    15181 atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac acatgcggtt
    15241 tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccgattac ctgaccgaca
    15301 cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta gtcatgcagg
    15361 ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg cttaaccgct
    15421 ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc acgggtgtag
    15481 taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt tttcagtgca
    15541 ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt ttcatcttcg
    15601 gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg aatgttcatg
    15661 gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc caggtagtgg
    15721 tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga ggccggatcg
    15781 cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg atttttcacg
    15841 cccactttcg ccattttctg gatcagttta cccggcatag aaccgatgta agtacggcgg
    15901 tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac atatttacgc
    15961 ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg cggccctacc
    16021 aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag gtactcaagg
    16081 atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc ctgcgcctga
    16141 cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat ccagtcgata
    16201 taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag cttctgcaac
    16261 tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat tttgcgcttc
    16321 agggcttcgt tttcgtccgg cacgtcgtcc atttcaccga gttctttctg aatagctttc
    16381 atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt aacgcggttg
    16441 cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc catcagatat
    16501 tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc cagtttcagc
    16561 ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct attcagcgac
    16621 gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa ctggctgatt
    16681 gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc cagatactcc
    16741 gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc gcgctgtaac
    16801 ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa tatagaggcc
    16861 acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc ttctttctgc
    16921 gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg gatagatttt
    16981 tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc gcgcagcggc
    17041 aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc tcttagttta
    17101 atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta acatgtacgt
    17161 cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa attaaagggg
    17221 agataaaatc cccccttttt ggttaactaa ttgtatggga atggttaatt attcaccaga
    17281 tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac catcaattac
    17341 cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt acatggtatc
    17401 gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac gcgccatcgc
    17461 tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc cttccagatt
    17521 aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga tctgaatcag
    17581 agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa tgaactcagg
    17641 gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct cgccttcgct
    17701 tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc cggtttctac
    17761 acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa acaggatctt
    17821 agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt gcggtggaac
    17881 agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtactcctt caccggaaac
    17941 gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt catcgatgta
    18001 gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca gcttctgaat
    18061 gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg cgtcggccat
    18121 ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt taccggaacc
    18181 ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc tggtatcgcc
    18241 gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt tcgcctgttc
    18301 ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg gtagcgcact
    18361 gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt tacataaatc
    18421 aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca cttcatgctg
    18481 gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc gtttatctgt
    18541 catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa atgccattct
    18601 caggcgcaag ccgcttacag cgttgtgccg ccctagataa gtatagcggc acagttgcgc
    18661 ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc caccgcttca
    18721 ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa tgattgaccc
    18781 gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc acgggcatga
    18841 atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat catcacgcgc
    18901 gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcaacaagaa agcgcccatc
    18961 gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg catggtgtca
    19021 tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag atagatatct
    19081 ttttctgggt tttccgcttc caggaacagc atctgcgcca caatcaggtt agccatgtgg
    19141 tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga atagatatca
    19201 aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc catatggggt
    19261 gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa attgaaaaaa
    19321 acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg tgttatgggt
    19381 acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca ccgaaagagg
    19441 cttttcgcac ggctccatgc aaaattcatg acaaaaaaaa gcccaccacc tggcggtgac
    19501 gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg gttcatcagc
    19561 tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc ttcaacagcc
    19621 tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt gctgtagaac
    19681 tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag gcctttcacg
    19741 cgctcttcgt cagctttcag ctcgttggtg cggataactt cgcccagcag caggccaaca
    19801 actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag agcttgtttt
    19861 tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc gctgtcgatc
    19921 agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc ctgagactta
    19981 acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac ttcagcgcgc
    20041 agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc agtcagttcc
    20101 ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc tttacctttc
    20161 aggttttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc ttcgccagct
    20221 ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc catcgccagt
    20281 acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc ggtgaagtcg
    20341 atggttacgc ggtcttctgc ttcaacagcg ccgtcttttt ctttccaggt cgcctgctgt
    20401 ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac gatcggtttt
    20461 tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc aaactctaca
    20521 gagtaagtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg tgcgccagcc
    20581 ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag gtcacccaga
    20641 acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg cactttgcct
    20701 ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct tttaacagcg
    20761 gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc ttgagtggtt
    20821 tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca actcttaccc
    20881 cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa tcacattccc
    20941 tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc ggcatcactt
    21001 ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt cccgggcaat
    21061 tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg gcccgcaggc
    21121 cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc aaatgcgttt
    21181 ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt cctgcaaccc
    21241 ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag tagagagttc
    21301 ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac ccgtaaaacg
    21361 atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat tgtgacgata
    21421 gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt tttcttctat
    21481 ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca tccatccctt
    21541 taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt taggtttcgt
    21601 cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac acaggcctcg
    21661 catcagcgtt tcttttacca ggggtgatta tttcgtcaac atgatgaatt tacatgcctt
    21721 acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc aaacacgctt
    21781 aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag ttgctctgtt
    21841 tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga cgattacact
    21901 gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg ccgatcagcg
    21961 taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc tgaccgcctc
    22021 ccgcgctctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg cgcgtggtta
    22081 catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac tgtatgctga
    22141 cgtgtcccag ggcaacgtgc gctacaacat cgctaccaaa gcggatatcg ccatcatcgc
    22201 taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca acgttgaagg
    22261 tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc tgaccgatac
    22321 catcgctgat atgtctcagg acaccagcat tcacgaattc atcaagcaga acgcgcgtta
    22381 atttctgccc tctggcccgg tgcaagccgg gcctgtagac gcccatgtcc agtcaatatt
    22441 tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt gcttccgggc
    22501 taccgctcgc cctgacatcc ggcaccttac aggcctggat gacggtcgaa aatatcgatc
    22561 tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa ttcctctggt
    22621 caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt tggctgctcg
    22681 ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa ccaggcaccc
    22741 aactccgctg gatggcggcg ctggcggtgg tgatcgcttt ttgctctgcc tcccaggata
    22801 ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt gcgggcgcgg
    22861 caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggccta gccctgtggc
    22921 tggcagataa atggttgggc tggcagggca tgtactggtt gatggcggcg ctgttgatcc
    22981 cctgtattat cgcgacgttg cttgcaccag aaccaaccga caccattcct gtgcccaaaa
    23041 cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat aatgcctggc
    23101 ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg agcctgacaa
    23161 ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg gtaaacaaaa
    23221 cgcttggctt actggcgacc attgttggcg cattgtacgg tgggattttg atgcagcgcc
    23281 tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct aacgctggtt
    23341 actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc gtctttttcg
    23401 aaaacctctg tggcgggatg ggcacatcag cctttgtcgc gctgttaatg acgctgtgta
    23461 ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct gtagggcgag
    23521 tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg acattctatc
    23581 tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc cgccagacgc
    23641 ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg gcaggttatg
    23701 cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg ttacttctgt
    23761 tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg ctggaagtcg
    23821 gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat ctggcgctac
    23881 gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca ctcgccgtaa
    23941 ttattacggc taaataaaca tcagcatcgc ttattaatat ttgttctttt gtgcggctta
    24001 gcgtttggaa attattgacg ccatttataa attctatttt tcttacacga ttcagctaat
    24061 gagtctttat tttctcacca cccagttgtc actttaatga taattatttg ttaaacaatt
    24121 gttttatttc gcattggtta taccaattgc ccaccaagac tccttctgca ctcccctttt
    24181 gttata
//
LOCUS       DAZXUL010000060        23393 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZXUL010000060 DAZXUL010000000
VERSION     DAZXUL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23393
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.060"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..343)
                     /locus_tag="ACH3L1_002387"
     CDS             complement(<1..343)
                     /locus_tag="ACH3L1_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077094.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
     gene            693..1715
                     /locus_tag="ACH3L1_002388"
     CDS             693..1715
                     /locus_tag="ACH3L1_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFT4077095.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1715..2695
                     /locus_tag="ACH3L1_002389"
     CDS             1715..2695
                     /locus_tag="ACH3L1_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFT4077096.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2692..3450
                     /locus_tag="ACH3L1_002390"
     CDS             2692..3450
                     /locus_tag="ACH3L1_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFT4077097.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            3460..4269
                     /locus_tag="ACH3L1_002391"
     CDS             3460..4269
                     /locus_tag="ACH3L1_002391"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFT4077098.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            4269..5123
                     /gene="modD"
                     /locus_tag="ACH3L1_002392"
     CDS             4269..5123
                     /gene="modD"
                     /locus_tag="ACH3L1_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFT4077099.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            5149..7103
                     /locus_tag="ACH3L1_002393"
                     /pseudo
     CDS             5149..7103
                     /locus_tag="ACH3L1_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(7153..7407)
                     /locus_tag="ACH3L1_002394"
     CDS             complement(7153..7407)
                     /locus_tag="ACH3L1_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFT4077100.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7608..8342
                     /locus_tag="ACH3L1_002395"
     CDS             7608..8342
                     /locus_tag="ACH3L1_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFT4077101.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(8344..8955)
                     /gene="emtA"
                     /locus_tag="ACH3L1_002396"
     CDS             complement(8344..8955)
                     /gene="emtA"
                     /locus_tag="ACH3L1_002396"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFT4077102.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            9055..9969
                     /gene="ldcA"
                     /locus_tag="ACH3L1_002397"
     CDS             9055..9969
                     /gene="ldcA"
                     /locus_tag="ACH3L1_002397"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFT4077103.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            10064..11800
                     /locus_tag="ACH3L1_002398"
     CDS             10064..11800
                     /locus_tag="ACH3L1_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFT4077104.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            12411..13825
                     /locus_tag="ACH3L1_002399"
                     /pseudo
     CDS             12411..13825
                     /locus_tag="ACH3L1_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13993..15063)
                     /gene="dadX"
                     /locus_tag="ACH3L1_002400"
     CDS             complement(13993..15063)
                     /gene="dadX"
                     /locus_tag="ACH3L1_002400"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFT4077105.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(15073..16371)
                     /gene="dadA"
                     /locus_tag="ACH3L1_002401"
     CDS             complement(15073..16371)
                     /gene="dadA"
                     /locus_tag="ACH3L1_002401"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFT4077106.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16701..18233
                     /locus_tag="ACH3L1_002402"
     CDS             16701..18233
                     /locus_tag="ACH3L1_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFT4077107.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(18285..19004)
                     /gene="fadR"
                     /locus_tag="ACH3L1_002403"
     CDS             complement(18285..19004)
                     /gene="fadR"
                     /locus_tag="ACH3L1_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFT4077108.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            19225..20766
                     /gene="nhaB"
                     /locus_tag="ACH3L1_002404"
     CDS             19225..20766
                     /gene="nhaB"
                     /locus_tag="ACH3L1_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFT4077109.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20912..21442
                     /gene="dsbB"
                     /locus_tag="ACH3L1_002405"
     CDS             20912..21442
                     /gene="dsbB"
                     /locus_tag="ACH3L1_002405"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFT4077110.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(21488..22756)
                     /gene="umuC"
                     /locus_tag="ACH3L1_002406"
     CDS             complement(21488..22756)
                     /gene="umuC"
                     /locus_tag="ACH3L1_002406"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFT4077111.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22756..23175)
                     /locus_tag="ACH3L1_002407"
     CDS             complement(22756..23175)
                     /locus_tag="ACH3L1_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFT4077112.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISYGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         5644 a         6115 c         5799 g         5835 t
ORIGIN      
        1 ggagaatgtt atcattgcta caccctgtgc ttaataactg caactctccg ccgttcagcg
       61 atgctttgat aattctgttc ccatagatat cgttatcaaa ataaagcgta aatatcatat
      121 ttccgtaact gttatcatct ctttttttgc acgtccgaat aaacgcattc agtaaattac
      181 tctctctaag aaaataaata tcttcattgt tgttaatgac attaataacg tcgttcttaa
      241 aatcctcaaa gtcttgttgc ggtaacttct ggttacgctg cttactatca gcaagcggtg
      301 aatccggacg acaacaacat aggttttgta taacccccag cattcttttc acctccgtta
      361 acagtatatt cacgacactg aaacgagaga atgctgataa ttttctcggc gagatatcaa
      421 aaatcgctca ttataaaaca agtaacttca acgtcgcgta accgtggacg ataaaaaaac
      481 aataatgtca ttcatattta aataatttag ccttaaatga tttaaataat attagccacc
      541 tgataaatac atttttaatg atagagaggg tcaacaggct tgcggaagtc gtcacatctt
      601 catgttttca atagctaaac tttgatattg aacatgattt ttatcgatat atatgaaagt
      661 atatatcgat tttacttttc aggatgttca ccatgcccat aaccagacgg acgtttgcgc
      721 aggcacttgc atctaccctg ctcctgcagt ctctaccgtc cttttcacaa acagttaatc
      781 gttttgcttc gcaatctctt cccgaagccc aaaacattac acgcatcgtc agcgcaggcg
      841 cgcccgccga tttgctcttg ctggctgtcg cgccagaaaa aatggtcggt ttttcctctt
      901 ttgattttgc ccgtcaggca ttaattccat tgccagagca cattcgccag ttacccaggc
      961 tgggacgact cgccgggcgc gccagcacac tatcgctgga agggcttatg gcgttacatc
     1021 ccgatttggt cgttgattgc ggcaatacgg atgaaacctt gatctcccag gcacggcagg
     1081 ttagcgaaca gacacaaatc ccctggttat tgcttaacgg aaaactggcg caatcagcag
     1141 aacagttaac aacgcttggc aaaacgttag gcgaagagca ccgcgccgca gaacaagcca
     1201 atctcgccag ccacttcgtt ggtgaagctc aggcattcgc cacctcaccc gccgctaacc
     1261 tccgctttta tgctgcgcgc ggtccccgag ggctggaaac gggcttacag ggttcgttgc
     1321 ataccgaggc ggcggaatta ttaggtttgc acaacgtcgc gcaaatagcc gatcgccacg
     1381 gcctgacaca ggtttccatg gaaaatctcc tgcgctggca gccggatatt attctggttc
     1441 aggaggccgt tactgcagat tttattcgtc gtgatcctct ctggcagggc gtgaaagccg
     1501 ttgcggaaca acgcatctta tttttaagtg gcttgccctt tggctggctc gatgccccgc
     1561 cgggaatcaa ccgtcttctg ggattacgca gacttcacgc ctggctggat cccgccatca
     1621 atcgccagtt taaaagtgac atgcagcatt acgcccaact gttctggcat tgttcactca
     1681 gtgatgccga ctatcaaaaa ttggtggcga gctaatgaga atcgtaaacg gatgtatttt
     1741 gctgctggca gctatcagta tcacttttgc cgccgtaagt ggggcttatc atctcgatat
     1801 gcagcaactg ctggcgctaa ttcttcgtca agaaaatgag tctgtgcagg aacaaattgt
     1861 cttctggcaa atccgcgtgc cgcgtattct tgctgcgctg ttactgggcg cagcgctggc
     1921 aggagccgga accacctatc agggaatgtt gcgtaatccc ttagtttcac cggatatttt
     1981 aggcgtctcc gccggtgccg gacttggtgc ctgcgcggca atcctgtggg gattacccat
     2041 tgtttacatc cagctgtacg cattttgcgg cgggctgatg gtggtcgcgg gcgtctggtt
     2101 gattacgcgt cgggtgaccc gccacgatcc gatcttaacc ctggtgctgg taggcattgc
     2161 gttaggaacg ttgtgcggag cggggatatc gctaattaaa acgctggccg atccttacac
     2221 ccagttaccg tcaattacct tctggttgct ggggggactg tccaccgtga ccttacgcga
     2281 tctctgctat gctgcaccga tcattctcat aggctccttg cccctgtttt ttctccgctg
     2341 gcgcatgaat ttactcacgc tttccgacga cgaagcccgt tcgctgggac tgaacgtgac
     2401 gcgtctgcgc tttggattga ttgtctgcgc cacactaatc accgccagca cggtagcaat
     2461 agcgggcata attggttgga tcggtctggt cgttccgcac attgcccgct tgttgaccgg
     2521 tcataaccat cagcagctgt tgcctatggc aatgtgtact ggcgctattc tgctcctgct
     2581 taccgatacg ctggcgcgca gcattggaac aacagaaatc ccacttggca ttctgaccgc
     2641 ttttgttggc gcaccgtttt tcctgctttt gttgttacgc ggaggtcgcc agtgactttg
     2701 cttgccgttc gtcatgcgtc tctgggttac tcccgccatc cagtgctacg cgacgtgtcc
     2761 ttcaccctgt cgcaaggaac aatttgttgt ctgcttggcg cgaatggttg cggcaaaacc
     2821 acactcatgc gctccattct tggcgttata ccgttattaa aaggcgagat cctactggat
     2881 agcctctctg tgcagacact cagccatcgc cagcgcgcac aggctattgc ctgggtgccg
     2941 caggcgcacg acggtatttt tgctttcagt gtgctggata tggtgttgat gggcctggcc
     3001 cccaccatag gcgctttttc cgttcccggt aagcaagaac gcctgaaagc cacggagcaa
     3061 ctggaaaagc tgggcatttt gcatctggcg acacgacgct ggaatacgct tagcggcggt
     3121 gaacgtcagc tggctttaat cgcccgtgca ctcgtccaac aaccgcgctt gttgttactg
     3181 gatgagcccg cctccagcct cgatttcggt catcagatcc aacttctgga cacgctggcg
     3241 cagctcaaaa ataacggcat gacaatgtta atgtcgactc accatacgct ccatgccaac
     3301 gccattgccg acagcattat ccaagtagag ccagatggac gagtaacaca aggcttaccg
     3361 accgaacagt taacaacgaa caaactggcg gccctgtatc gggtttctgc cgaccaaatt
     3421 catcatcacc tctctgcaat tagccgctaa ggaacaatca tgctcattga tgatattgat
     3481 tttgcagacc tttatctgca gcaactcaaa ctggctcacc gcacagaaaa aacgcccgat
     3541 cactgggatc aacgggcgga gaaaatggcg gaaaattgcg cctcgccaac ggacagctac
     3601 ctgcaacaac tgatcgctaa aattgacctg caaggcgctc agaccttatt cgatatgggc
     3661 tgcggaccag gcaccgtatc gctggcgctg gctgacaaac tcactacggt ttatggcgtt
     3721 gattacagtc aggggatgct gaacgttgcc gcccgccgcg ccgccgcact gaaggctgac
     3781 aatgtccact ggatccagcg cgcctgggaa gaggactgga gtgacctgcc acgctgcgat
     3841 attgccgtgg cttcccgctc cactctggtg gctgatatgc gccatgcaat gagcaaactg
     3901 aataatcagg cacggcttcg cgtctataca actcatctgg tcagcacctc gttcgtctct
     3961 ccagccattc agcgggcggc tggtcgggaa gtgatcgaat tacccaacta tatctttgcg
     4021 ctcaacgtcc tgtatcagat gggaatttat gcccacgttg attttatccg tggacaaaat
     4081 tgccaacagg ataacagcac ctgggaacgt tttgagcaga acgtcagctg gagtcttggc
     4141 gcgcttaacg acgatgagcg tgagcggtta tatcactggt atcaacaaca agatgctcgt
     4201 gccctcgccc ctgccagccg cgactgggcg ctcatctggt gggatagcgt accgcaggag
     4261 gcactgtgat gattttcctt tctcaggcac aaatcgatgc gttactgctg gaagatatcc
     4321 agggaggtga cctgaccacg cgagcattaa atattggaca ccagcctggc tatatagcgt
     4381 tttttcaccg tcagggcggc tgcgtcagcg gcgtttctgt cgcgtgtaag atgttaacta
     4441 cgctgggatt aactattgat gacgcggtca gcgacggttc acaagcgaac gcgggtcaac
     4501 ggctaatccg tgcgcagggt aatgccgcag cacttcatca gggatggaag gcagtccaga
     4561 atgtgctgga gtggagttgc ggtgtttctg attatctcgc tcaaatgctg gcattgcttc
     4621 gtgaacgtta tcctgacggc aatatcgcct gcacccgaaa agcaattccg ggcacccgct
     4681 tactggcttc gcaggcaatt ctggctgccg gaggactaat tcatcgtgcc ggatgtgcgg
     4741 aaaccatatt attgtttgcc aaccaccgcc attttcttca tgacaatcag ggctggtcag
     4801 gcgcaatcgc tcagttacgt cgccacgcac ccgagaagaa aattgttgtt gaagccgaca
     4861 cgccgaaaga ggctatcgcc gcgttacgcg cacaaccaga cgtacttcag ctcgacaaat
     4921 ttagtccgca acaggcaact gaaattgcgc aaatagcgcc gtcgctggct cctcactgca
     4981 cgctggctct gaccggcgga attaatctga cgacactcaa aaattacctc gactgcggca
     5041 ttcgcctttt tattacctcc gcgccttatt acgcggcacc agctgacatt aaagtcagtc
     5101 tgcaacccac agcctctatt taattctttg ttaaacggaa tttattctat gagactcaaa
     5161 aaacgttatc tctgcacagc attatcactt gcctttaccc agcaggccgt agcggctcag
     5221 gagagcgaca cgctgaccgt atggtccagt ccggtatcat cgacgacgac caccgttctc
     5281 gatcaaccca ccatgaaggc cttggataaa cagaatgtcg ctcaggcatt aagtgtcgtc
     5341 cccggcgtgg tgctgcaaaa gtcaggcagc cgcaacgaag aacaggtaaa agttcgtggc
     5401 tttgatagtc ggcaggtgcc ggtctatttc gacggtgtgc ccatttatgt cccctatgac
     5461 ggcaacctcg atctggcgcg gattctgacc aacaatctgg gggcagtcga agtttccaaa
     5521 gggtattcgt cgctgcttca tgggcctaat cagatgggcg gagccattaa tatcaccacc
     5581 ctgaagaagc aagtctggga tatcgccagg gatggagccg tagtcaggac aatgcctacg
     5641 atatgcatgc ttcatttgcc gccagcagcg atcttgggta tttgcaagtc agcggtagcc
     5701 agctaaagca ggattttctc ggcctgccgc atggtgtaaa taatgatatt gcaggcaaac
     5761 acggcaagat gattaattca tcggctgatg ataaacgcgg cattgtgaag ctcggtttta
     5821 caccgcgcga aaacgatgaa tacacgctga cttacattaa gcaggatggt gaaaaagata
     5881 acccgccata cagcggaaat tgtggtcaaa aatcacgcta ctggcagtgg ccagagtatg
     5941 acaaagaaag tttttattat cagggagcga cccaactaaa cgaccgtttt accctgaaaa
     6001 gtcggctgta tcgcgacacc tttgaaaata cgctgatgat gtacaactcg ctggctgatt
     6061 tgaaaaataa aaaaggcagc tacagccatt attccgatta cagcgacggt gccggattac
     6121 aactggcagc cgatgtgcgt gaaaacgatc tgctgtcgtt tgccgttaac tggaaagatg
     6181 acgtacaccg ggaaaaaggt gcgccgcacg ccgcttacga tcgctatgaa gatcgtacct
     6241 ggtcgctcgc cagtgaatat caatgggctg ctgctgataa tgtcgatgtc gtggcgggaa
     6301 tcagctatga ctggcgcgat agcgtagaag cgaaaaaaca cgagaaagat ggcagcatca
     6361 cccattatga cgacaacaat cagtcagctt ttaactggca ggtgatgggg aaataccact
     6421 ttgccaatga agacactctg gcgctttcgt actatgaccg cacacgcttt ccgacgctga
     6481 aagaacgcta taccacatcc aaacctgcgt ataaccagat agcgattgtt aacccgcagc
     6541 tcaagccgga acgcgctcgc ggggtggatt taacctggaa tggtgccttc acgcgcgact
     6601 ggggatttga ggtcagcgtt tactataacc gggtgagtga tgccatcctc tcgcacaata
     6661 tcaatgccga taccattcaa aatcagaaca gcggcacggt ggattacagc ggtctggatg
     6721 ccggtattaa ggggaaaatc agcaatatac tggatgtagg attgagctac gctctgatcc
     6781 acgctgacgc caaacgcaaa gacatcggca agataaccga tcttccaacg cagacaatga
     6841 ccgcatggat gacactcaaa ccatgggagc cgttaagcgt aacgctgtcg gaagaggcgc
     6901 gttcctccag ctacagcaac agtgacggtt cacaaaaagc cgccggtttt gcggtgaccc
     6961 acattcgagc cgattacacc ttaggtcatg gcttcagcgt caatgcgtcg gtcaataacc
     7021 tgtttgatac ccaatatgcc tacagtgaag ggtttattga agaaggtcgc aatttctggg
     7081 caggtgtcga gtatacgttc tgatgtgaaa gcggatagcg gcatctgtat cgctatccgc
     7141 cttatgaaaa tcttattctc ttcgcaggag gcggaatacg cccagcacca ggatagcgcc
     7201 caccaccgcc accaggaagc tgtgcaaatt aaaaccgctg atggagccgt caatgccaaa
     7261 catggtcgcc agccagccgc cgaccaccgc accgactatc ccgagaatac aggtcaggaa
     7321 aaatccacca ccatcacgcc ccggcatgat tagcttgccg ataatgccgg ctatcaggcc
     7381 aaaaataatc caggcaataa ttcccatgtt caggccctcg tttcgatgaa tcggtcgcaa
     7441 gaaatgctta cgtcaacgct ctaagtatag gcaagggcgg gatcgatgct gggcgtttgt
     7501 cggctgattt aagaacaaca tgtaaaaaat attcacgcat ttgtattaag ttttgttaac
     7561 tatgaccgat aaaccaaaga cagtttgtca gtcaggagtt tttccgcgtg agtcattacc
     7621 atgagcagtt cctgaaacaa aatccgttag ccgtcctggg cgtgttacgc gatttgcaca
     7681 aagccgcaat tcctttgcgt ctcagttgga atggcgggca gctgatcagc aaaatactgg
     7741 caataacccc ggataaactg gtgctggatt tcggcagtca agccgaagac aacatcgccg
     7801 tgctaaaggc acagcacatt accattaccg ccgaaactca gggtgcgaaa gtcgagttta
     7861 ctgttgaaca actacagcag agtgaatact tgcagcttcc ggcatttatt accgtaccgc
     7921 ctcccacctt atggtttgta caacgacgcc gatatttccg catctccgcc ccactccatc
     7981 cgccttattt ttgccagacc aaactggcgg ataacagtac gttacgtttc cgcttgtatg
     8041 atttgtcgtt aggcggcatg ggcgcattac tggaaacagc aaagcctgcc gaattacaag
     8101 aaggtatgcg cttcgctcaa attgaagtca acatggggca atggggtgtt tttcactttg
     8161 acgcccagtt aatctccacc agcgagcgca aagtgattga tggcaagaat gaaaccatca
     8221 ccactccccg tctgagcttc cgttttctta acgtcagccc gacggtggag cggcaattac
     8281 agcggattat tttctctctc gagcgagaag cccgggaaaa agcggacaaa gtgcgcgact
     8341 gatttacatc gcgtccagtg cctgctcaag tttgtagata tagcgcggag cctgcggcgc
     8401 aggatgattt cgcgctacgt gatcgaggaa ctcgtcagca tccaaatcgt tgattttgct
     8461 gatcgccttt ttccgatctg acgagaaagt ccgtagcagc gcacctgccc cgttagcgta
     8521 tgacaccacc agcgcatatt gcagtacctt cggatcttca atgcctgcca gcgggccggt
     8581 ttccagaata ttcaggtaag ccgcccccat tgaaatatta cgctctggat ttttcagctc
     8641 gctggtcgtc ggctcaccac tccagcccat acggcgataa acatcacgtc cggaggttga
     8701 agcttttaac tgcatcaaac caatggcatt cgatttactc accgcgttcg gattaccacc
     8761 cgattcgata gcgataatcg ccgtgatcaa ttgtggatcg acgccccagg ctgcaccggc
     8821 tttctggctt attggcatcc actgcatcgc acgttgcacc ggaactttcg cgttccacgg
     8881 cgggttcgta tagtcatgct ttgatgaaca acccgctaat aacacaatca aaaaggcaaa
     8941 ccatctcaat ttcacttctt ctatccttat agcctggaat cgccgttgag gtgacaacgg
     9001 cacacctaag cggcatgata tacatttcgt ttctgttgtc agcaaggaat tgccatgtct
     9061 ctgtttcact taattgcccc atcgggttac agcattaaac agcacgccgc gctgcgtggt
     9121 atccagcgcc ttaccgacgc ggggcatcaa gtcaataacg tcgaggttat tgcccgtcgt
     9181 tgtgagcgtt ttgcgggtac ggaaacggag cgtctggaag atctcaattc ccttgccaga
     9241 ctgactaccc ccaacaccat cgtgctggct gtacgcggcg gttacggtgc cagtcgttta
     9301 ctggcagata ttgactggca ggcactggtg gctcgccaac aacatgaccc gttactcatt
     9361 tgcggacata gcgattttac cgccattcag tgcggtcttc tggcgcaagg caatgtcatc
     9421 acctttagcg gccccatgct ggtagcaaac tttggtgcgg atgaactgaa cgcctttacc
     9481 gagcaccatt tctggctggc gttacgcaat gaaaccttca ccattgaatg gcaaggggaa
     9541 ggcccgacat gtcagaccga aggcacgttg tggggaggca atcttgcgat gctgatttca
     9601 ctgattggta caccgtggat gccaaaaatt gagaacggta ttctggtgct ggaagatatt
     9661 aacgtgcacc ctttccgcgt cgaacgtatg ctgttgcagc tctatcatgc gggaattttg
     9721 ccacgtcaga aggcgattat tctcggtagc tttagcggca gcacgcccaa tgattatgac
     9781 gcaggttaca acctggagtc agtgtacgcg tttttgcgtt cccgcctgtc gattccactc
     9841 attaccggcc tcgattttgg tcatgaacaa cgcacggtta cgctgccgtt gggcgcacat
     9901 gccattctga ataacacccg ggaaggcgct cagttgacga ttagcggtca tcctgttctt
     9961 aaaatgtaaa aaaatcacgc ttcagggcta agtaaaacgc tgtctctgcc gctaatatgg
    10021 tagggtttat ataagagaca gcgtaatcag gagtaaccga ccattggatg ccacaacaat
    10081 aattagcctt ttcatcttag gatccatcct tgtcaccagc agtatattac ttagttcatt
    10141 ttcttcccgt cttggcattc ctattctggt tatttttctg gcgatcggca tgctggcggg
    10201 agtcgatggc gtcggcggta tcccgtttga taattacccc ttcgcctaca tggtaagtaa
    10261 cctggcgctg gcgattattt tgctcgacgg cgggatgcgc actcaggcca gttcctttcg
    10321 tgtggcgtta ggaccggcgc tgtcgctggc gacgctgggc gtacttatca cctctggttt
    10381 aaccggcatg atggcggcgt ggctgtttaa tcttgatttg attgaaggct tattaatcgg
    10441 cgctatcgtc ggctccaccg atgctgcagc ggtcttttct ctgctgggtg gtaaggggct
    10501 taacgaacgt gttggctcga cgctggaaat tgaatccggc agtaatgatc caatggcggt
    10561 ctttctgacg attaccctga ttgcaatgat ccagcagcat gaaagcagtg tcagttggat
    10621 gttcgtggtc gatattctgc agcaatttgg cctcgggatt gtcatcggtc ttggcggtgg
    10681 ttatttactg ctgcaaatga ttaatcgcat cgccctgcct gccggattat atccattgct
    10741 ggcattaagc ggcggtattt taatttttgc tttaaccacg gcgctggaag gcagcggtat
    10801 tctggctgtt tatctgtgcg gttttctgct gggtaatcgg ccgattcgca accgctacgg
    10861 catcctgcaa aatttcgacg gcctcgcctg gctggcgcaa atcgccatgt tcctggtgct
    10921 ggggctattg gttaacccaa gcgatctgct gcctattgcc attccggcgc tcattttgtc
    10981 ggcatggatg atattcttcg cccgtcccct ttcggtattt gctggattgc tccctttccg
    11041 cggtttcaat ctgcgtgagc gcgtgtttat cagctgggtg ggattacgcg gcgcggtgcc
    11101 aatcatccta gccgtgttcc cgatgatggc ggggctggag aatgcgcgac tgttctttaa
    11161 tgtcgccttc tttgtggttc tggtttcact gctcttgcag ggaacatcac tctcgtgggc
    11221 ggcgaaaaaa gccaaagtgg tggttccgcc cgtcggacgt ccggtgtcac gcgttggcct
    11281 ggatattcat ccggaaaatc cgtgggagca gtttgtttat caattgagtg ctgataaatg
    11341 gtgtgtaggt gcggcactgc gcgatttgca tatgccaaaa gagacgcgta ttgcagccct
    11401 gtttcgtgat aaccagttgc ttcatcctac cggcagcacc cgactgcgcg aaggcgatgt
    11461 attgtgtgta attggtcggg aacgcgatct cccggcgctc ggtaaactgt tcagccagtc
    11521 gccaccggtc gcgctggatc aacgcttctt tggtgacttc attctcgaag ccagcgccaa
    11581 atatgctgat gttgcgctga tatatggcct ggaagacggg cgagaatatc gtgataaaca
    11641 gcaaacgctg ggtgaaattg tccagcagtt gctaggcgcg gcaccggttg tcggtgacca
    11701 ggtagagttt gccgggatga tctggacggt ggccgagaaa gaagataatg aagtgttgaa
    11761 gattggcgtt cgggtggctg aagaggaagc tgaatcttaa atttaatgca gcatctatcg
    11821 ccggatgcga tgctttactg aacgcagcct gagcctgtaa taaacttgtc atttcctgat
    11881 tttgatatgc tcatcccaac atcagaagaa ggttaaatta tggatgacaa accattgcag
    11941 gctctggcta atgaactcaa aacaccggct caagaaagtc agcgtaaggg ctttttccga
    12001 ctgtaacagg taataaaaac cagccccgca ttaccggtca accatcaggg gcccctgcct
    12061 gacatcgcct gaacatattc ccccacgcaa cctgccgcac tgacatagcc cacacaatag
    12121 gcgtttcatc cgcctgtagc accggatgca tgagcgattt cttatagtta ttcctttgct
    12181 taataacgat tatttccggg tcgttcactc tcgtattttc tttaatacgt cagagctacc
    12241 taggccgtaa tacagcatgt accagacccg acacggcatt tctgaccagg aaaaaagtca
    12301 acgatgagta gtgatatcag cgcaaatgac gtcctgtggc tcctttgcgg tgaccacatt
    12361 cacgtctccg gcgctgccag aggatattta tttcaaggaa acgtagcatt atggctagct
    12421 ttaaagacat ctgcccattc gcccccgcca ttcccccatc ttccgttaac ccaggcgtac
    12481 gacgcccgcc agggcggctc ttctcaagaa tcaaattttg cagaacatga agtatctcag
    12541 cccgcaaaaa ttcagctggg gtgatgcccc ctggcagatc attgacctca gtatcgcggg
    12601 caaagtgaac atacaggtgg acaacaatac catcatcacg ttggggactc gtctgaatca
    12661 gcaacataac gagttcatga tggtcgccaa atggtgcgaa tgggctatac agcaggatgg
    12721 tttgcaagaa aacctgcaaa aaaacctgta cgaaatcctg gaagaaaacc agcaaaacaa
    12781 acagtcagaa attccccaag aagacctgaa agaaagtctg gaagaaatca aggaaaatat
    12841 cctggaagaa aacctgccag caagccggat tgaaaaccgg gcagaagcat tgcgcagaat
    12901 gaaggaatgt ctcattacaa gacggagtat gctcaatctt agcaacctgg gattgacttc
    12961 actccctgag aatttgcctc cacatctgat tgaattttac tgcagtaaaa acgtgttgac
    13021 cgcgttaccg aaggtgatgc caaagtggct gctggtgctt gactgtacag ataacgtctt
    13081 gatcttgtta ccgaaggtgc agccctcaaa actgatggta ctgaagtgct atgaaaacag
    13141 tattatctgg ctgcctgagc tgtcgactaa cctgagagtg attaactgtt ctgaaaactt
    13201 cttgcaattt ttaccgccgt cgatgcccca gtacctgtat aaactatcct gtgccggcaa
    13261 caatattaac tcaatacctg atgagatgct ggagaacttg actcgcctga aagtatttga
    13321 ctgttctagt aacgatttga tctcttcacc acggctgccg cccaaactaa taatttacta
    13381 ctgtggagaa aacaagttta aaacggtaca ggtgccgcag ccccagagcc tgaaggtgtt
    13441 tgactgtaat ggtaacccgt gggacaaaga caatttaccg acgctgctca aagccgtcga
    13501 gggcctgaaa aaacagcagg gcctgaagga ccttttggac tttttgcaca aggaaggcta
    13561 ggttgacctg gaaggactcg aggagctgga ggaccttatg gatctggagt tcctggatga
    13621 cccggaactc ctggagcgcg tgaaggtaca ggaggacctg gagctcctgg atcaacagtt
    13681 gggcctgttg agtctggaaa gacaacagga ctcgcagcct gttaatcaac aatctgaaca
    13741 tgaacccgaa tctgcatcaa gggtgaagcg tgatttatct gaagtcgact ccgagtcaac
    13801 aatgaagcgt aagcgtttta tgtagaacgc ctgtgcggcg agttcaggtt ccggggaaat
    13861 agtgtccatt cttttaatgg acactatcat gggcaatggc tctgtcacat taacaactac
    13921 agttgctgac cagccggatg gatgattttt tcgcaaccag aagacgctgc ctccgatccg
    13981 gcttacaaca agttacactg tcacaaccgg gacgcgtaac gccagtgcgc acatcaactc
    14041 atagcccacc gttccggcag cggcggcgac atcatcaatt ttgatctcct tgccccacag
    14101 ctcaaccggc gtaccaatcc ccgcctgcgg gcaaggcgtt aaatcaaccg ccagcatatc
    14161 catcgagacg gtccccaccg tcatggtgcg cacgccgtcc actaaaacag gagcaccggt
    14221 aggcgcatgg cgcggataac cgtcggcgta tcctgcggcg acaatgccga ttcgctgttc
    14281 atcgcgcgca gtatagcgac cgccgtagcc cacacgctcg cccgctttta gcgtctggac
    14341 accaataatc tcactgctta gcgtcatcac cggacgtaat ccggtattgg cgatatcacg
    14401 ccactgaccg gacggcgaag cgccatacaa aataatgcca ggccgaaccc agtcaaaatg
    14461 cgcttccggg tgccacagag tcgccgccga attggacaac gaacgccgac actccagccc
    14521 ctccgccgcc tgctcaatac gcgccatcgc gccggaaatt ccatcaggat gttccgcttc
    14581 agcaaaatgc gacatcaggg tcatttcgcc aacattcgcc attgcccgca actgctgcca
    14641 gacggtaagc acgcgatcgg actggaagcc caaccgattc atcccactgt tcactttaag
    14701 ataaatatcc aacggtgctt ttagccgcgc attttgcagt gctttgagct gccagttgct
    14761 gtgtacgcag gtggtcaggc ggtgctggtc ataaatctcc agatcctgag catggaaaaa
    14821 tccttccagc atcaggatcg gccccttcca gccgcgctca cgtaacgtta ttgcctcttc
    14881 caggttaagt aatgcaaagc catcggtggc cccgagcgcg ctccagatac gctcaatacc
    14941 atgcccgtaa gcgttcgctt ttaccaccga ccagacgcgc gcgtgcggcg cggcctggcg
    15001 gacaatggac agattctgtt ttaatgcctg cagatcgagg ctggcctgta tcggacgggt
    15061 catctcgttt ccttagctgt gtgcgccatg taaatggccc ggacgtgatg gcgtaaatcc
    15121 acggctgtag cgcgctacgc ttagatcctc atatgggatc gctggcgtgc gaccagagag
    15181 cagatcgctt aacaactgac cggaaccgca agccatcgtc cagccgagcg tgccgtgacc
    15241 ggtattcagc cacagatttt taaagcgtgt actcccgaca accggcgtac cgtctggcgt
    15301 catcgggcgc aaacccgtcc agaaagtcgc ctgctcgaca tgaccgccgc gtggatagag
    15361 atcgcgaacc accatttcca gcgtttcacg acgcggttgc aacagctcgg tgttaaaacc
    15421 aacaatttcc gccattccgc caacacgaat gcggttatcg aaacgggtaa tggcgatttt
    15481 gtaggtttca tcaagaatgg tggatactgg cgcaccatct tcctgcgcaa ttggaatagt
    15541 cagcgagtag cctttcagcg gatacaccgg aatatcaaca atgcctttga gcatcgccgt
    15601 cgagtaagaa ccaaacgcca tcacatacgc atcggcctta atcacttcat cgccaaactt
    15661 cacgccgtag atttgctcgc cgtcgcaaag cagttggtca acgggcgtat taaagcggaa
    15721 tttaaccccc gcctgctctg ccatccgcgc cagattctgg gtaaacagct ggcagtctcc
    15781 ggtttcatca ttgggtaact gcaggccgcc cgtcagtttg tgcgctactt ctgccagcgc
    15841 gggatccact tccgccaggc ggctggattc cagcagctga tacggtacgc cggcatcttc
    15901 cagcacggcg atatcgcggg tcgcattttc atactgttgt tcggtacgga acagttgcag
    15961 cgtcccaccc tgacgccctt catactgaat attggtttcg gcgcgcaatg ctttcaggca
    16021 atcacggctg tattccgcca gacgcaccat ccgccctttg ttttccatgt agtggctggt
    16081 gtcgcagtta cgtaacattt gccacatcca tttcaactga aactgcgtac cgtcgagacg
    16141 aaccgccagc ggcgcatggc gctggaacat ccatttaatc gcttttaaag gcacacctgg
    16201 tgctgcccac ggtgcagcat atccggggga gatttgcccg gcattcgcgg cactggtttc
    16261 cagggcggcc cccggctccc gatcaatgac ggtgacctca tgtcctgcct gatttaagta
    16321 ccaggcgctg gcaacgccta ccacaccact tcccagtatg acaactcgca tagccactcc
    16381 gataacggta gatacagtaa aagaataata atctaattac atcttgataa ctcagttgaa
    16441 aatattattc agcatacggc ttttttatgg tgagctgact cacatctccc tgactctgcg
    16501 gcaatatggc gtctttagta tctcctgctg aacgcccttt ttatccagca taaaattccg
    16561 ttcagaagcg gattagtggc actctgataa taagaaatcg caaagaaaaa aattctccgg
    16621 cgacgcggtt tttaacaggt gttctatgct tgaaatgagg tgctccacac agagagcgcc
    16681 gacaacaatg aggtgcgcgt atggcgacga tcgattctat gaataaggac accacacgtt
    16741 tgagcgatgg acccgactgg acgttcgacc tgctggatgt ttatctggca gagatagacc
    16801 gggtggcgaa actctaccgg ctggatacct acccgcacca gattgaagtg ataacctcag
    16861 aacagatgat ggatgcctat tccagcgtcg gcatgccaat taactatccg cactggtcat
    16921 tcggtaaaaa gtttatcgag actgaacggc tgtataagca cggtcagcaa gggctggcct
    16981 atgaaatcgt cattaactct aacccgtgta tcgcttactt gatggaagag aacaccatta
    17041 ctatgcaggc gctggtgatg gcacacgcct gctatggaca taactctttc tttaaaaaca
    17101 attgcttatt ccgcagttgg accgacgcca gttcgattgt cgattatctg atttttgccc
    17161 gtaaatatat taccgagtgc gaagaacgtt atggcgttga tgaagtagaa cggcttctgg
    17221 actcgtgcca cgcgctgatg aactacggcg tggaccggta caaacgcccg caaaaaatct
    17281 cgctgcaaga agagaaagcc cggcagaaaa gtcgcgaaga gtatctacaa agtcaggtca
    17341 atatgctctg gcgtaccctg ccgaagcgcg aggaagagaa aacggttgct gaagcgcgcc
    17401 gctatccgtc cgaaccacaa gaaaacctgc tctattttat ggagaaaaat gcgccactgc
    17461 tggaatcatg gcagcgtgaa atcctgcgta ttgtgcgtaa ggtgagccag tatttttatc
    17521 cgcaaaaaca gactcaggtg atgaacgaag gctgtgcgac cttctggcac tataccatcc
    17581 ttaaccatct gtatgatgaa gggaaagtaa cggaacgttt tatgctggag tttttgcaca
    17641 gccacaccaa tgtggtcttc cagcccccct ataacagccc gtggtacagc ggcatcaacc
    17701 cgtatgccct cgggttcgcc atgttccagg atattaaacg gatttgtcag tcgccaacgg
    17761 aagaagacaa atactggttc ccggatatcg ccggttccga ctggctggaa acgctgcatt
    17821 tcgcgatgcg tgatttcaaa gatgagagtt ttatcagcca gtttctgtca ccgaaagtga
    17881 tgcgtgattt ccgcttcttc accgtgctgg atgatgatcg gcataattat ctggagattt
    17941 ccgctattca taatgaagaa ggttatcggg agatccgtaa ccggttatcg tcgcaatata
    18001 acttaagtaa tctggagccg aatattcaga tctggaacgt ggatttgcgc ggcgaccgtt
    18061 cgctgacgct gcgttatatt ccacataatc gcgcaccgct ggatcggggg cgcaaagaag
    18121 tcctgaagca tgtgcatcgc ctgtggggat ttgatgtgat gctcgaacag caaaacgaag
    18181 acggcagcat cgagttgctg gaacgttgcc cgccaagaat gggaaatctg taaaaacaac
    18241 aaaaaacccc tcgtttgagg gggttgctct ttaaacggaa gggattatcg cccctgaatg
    18301 gctaaatcac ccggcagatt tttctgcatc cggtgccaaa tctcgccact ctcatgccca
    18361 tagcgacgca ctgtttcgta cacctgatcg tgcgcgcctt cactgcacaa cgccgacagt
    18421 ttgtggtaga agcccagcgc cagactgcgc gcttccggat tggcgaaata gtgacgacca
    18481 atgcgcgtat acagcccttt catcccgtta aggatcagac cgtaaatcgg gttgccggaa
    18541 gcaaacgcca ggccgcggaa tatgttgtaa tccagctcgg caaaggcatc ggcgtgatcg
    18601 gccacttcat tagcggtagc cagcacttcc tgcgctttat cgggatgctg acgaaacgcg
    18661 gtgcgaataa aaatagtgga aatattggta cgcaccgaca gcaaattatc aataagctgc
    18721 ggcacacttt cgtgatccag tcgcgccagt gtttcaagga tatttaaacc ggaagtttcc
    18781 cagaaattat tcaccttcgt cggcttgcca tgttgaatgg tcaaccagcc atctcgtgcc
    18841 agacgctgta acacttcacg taacgtagta cgcgttacgc caattaattc tgaaagttca
    18901 cgttctgcgg gcaaaatagt cccgggaggg aagcggttat tccagatact ttcaataatg
    18961 tactcttccg cgaaacccgc cgggctttgc gccttaatga ccatagtgag atttccataa
    19021 cacagcaaaa caaagttgga ctcatcatac cagagggcta actacgctga tagcagacaa
    19081 aaagaagaaa aagggatcaa ggcttcattt tccgcatctc tttccccttg actatcaaga
    19141 cttgcctcac tcctgcctga ccgttaagct ttgcgtttaa acataaaaac atcattgatt
    19201 atttgtaagg taagggaaac catcatggag atctcctggg gccgcgcgct atggcgcaat
    19261 tttttgggcc agtcccccga ctggtacaaa ctcgccctca tcattttctt aatcgtaaac
    19321 ccgttaattt tcctcatcag ccctttcgtc gcgggctggt tgctggtagc ggaatttatt
    19381 ttcactctgg cgatggccct gaaatgctac ccgctactcc ccggtggtct gttggctatc
    19441 gaagcggtat tcatcggcat gaccagcgcg gaacacgtcc gtgaagaggt ggcggcaaat
    19501 cttgaagtct tgctgttatt gatgtttatg gtggcgggta tctattttat gaaacagctg
    19561 ttgctgttca tatttacccg tttgctgtta agcattcgct ccaaaatgct gctgtcgctc
    19621 tctttttgcg tggcggcagc gttcctctcc gcgttcctcg atgccttaac cgtcgtggcg
    19681 gtggtgatca gcgttgcagt cggtttttat ggtatttatc atcgcgtagc ctcttcccgt
    19741 accgaagaca ccgacctgca agacgatagt catatcgaca agcattacaa cgtggttctg
    19801 gaacagttcc gtggctttct gcgtagcctg atgatgcatg ccggtgtcgg caccgcatta
    19861 ggcggcgtaa tgaccatggt gggcgaacca cagaacctga tcatcgctaa agcggctggc
    19921 tggcattttg gcgatttctt cctgcgcatg tcgccggtga ccgttccggt tctgatttgt
    19981 ggcctgttaa cctgcctgct ggtagagaag ctgcgttggt ttggctacgg tgaaacgctg
    20041 ccggagaaag tccgcgaagt gttgcaacag tttgacgatc aaagccgcca ccagcgtacc
    20101 cgtcaggata aaatccgtct gattgtccag gcgattattg gcgtctggct ggtgactgcg
    20161 ctggcgttgc atctggcgga agttggcttg attggtttgt cagtcattat tctggcaaca
    20221 tcattgaccg gtgtcaccga tgaacatgct atcggtaaag ccttcaccga atctctgcca
    20281 ttcaccgcac tgttgacggt gtttttctcg gtagtcgcgg tgattatcga ccaacaactg
    20341 ttttcgccga ttattcagtt tgtgttgcag gcatctgaac atgctcagct gtcgctgttc
    20401 tatattttca acggtctgct gtcatccatt tcggataacg tcttcgtggg gacgatttat
    20461 atcaacgaag cgaaagcggc aatggaaagt ggcgctatca cgttgaagca atacgagctg
    20521 ctggcggtcg ccattaatac cggtaccaat ctgccctctg tcgctacgcc gaacggtcag
    20581 gctgcgttcc tgttcctgct gacctctgca ctcgcgccat tgattcgcct ctcttatggc
    20641 cgcatggtgt ggatggccct gccttacacc ctcgtactga cactcgtcgg cttgctctgc
    20701 gtcgagttta cgcttgcccc tgtaaccgaa tggtttatgc aaatgggctg gatagcaacg
    20761 ctttgataac aacttaccgg gcatataaat gcccggtttg ccttttcgcc cgataattgt
    20821 ccaattgcgt cctatttttg ctgcctcctg gtggcgcagc gaatgaattg gtttaaactg
    20881 cgcactctat gcatattgca gggaaatgat tatgttgcga tttttgaacc aatgttcaca
    20941 aggccggggc gcgtggctgt tgatggcgtt tactgctctg gcactggaac tgacggcgct
    21001 gtggttccag catgtgatgt tactgaaacc ttgcgtgctc tgtatttatg aacgctgcgc
    21061 gttattcggc gttctgggtg ctgcgctgat tggcgcgatc gccccgaaaa ctccgctgcg
    21121 ttatgtagcg atggttatct ggttgtatag tgcgttccgc ggtgtgcagt taacttacga
    21181 gcacaccatg cttcagctct atccttcgcc atttgccacc tgtgatttta tggttcgttt
    21241 cccggaatgg ctgccgctgg ataaatgggt gccgcaagtg tttgtcgcct ctggcgattg
    21301 cgccgagcgt cagtgggatt ttttaggtat ggaaatgccg cagtggctgc tcggtatttt
    21361 tatcgcttac ctgattgtcg cggtgctggt ggtgatttcc cagccgttta aagcgaaaaa
    21421 acgtgatctg ttcggtcgct aatccattcg gcgctcctgc gggagcgctt ttttcctgcc
    21481 gctatattta tttgacccgc agtaaatcag aacttcgcgt tgtataacgt ggtgaaagca
    21541 tagctcgctt catctgccat tgttgttgga tcccctgccc ggcaaaatag agtgttcctc
    21601 tgccctcttt agcattcaga gtatccatta ccgccatcaa ttgctcactc ccggggcgcg
    21661 gtgcgttgtc atcgaataaa ttgagctgcg cgactccctg actgaagaaa tcccccagca
    21721 tcacgcccgc tttttggtag cgatggcccg cttgccagat ggcatccaga gatcgcgtag
    21781 cagcgttaat gatatccctg ctgtcctgag tgggcgtcag cagttttacc gacgcgctat
    21841 tgccgtaata aggttcattg agcgcaaatg gtgacgtctt aataaatgtg gagataaacc
    21901 gacaatattg atgctcactg cgaagttttt ccgccgcccg ggcagcgtaa ctacaaatgg
    21961 cctgccgcat cgacgtataa tccgtgatgc gttcaccaaa cgagcgggaa cagataattt
    22021 cctgcttcgt cggtgcaaac tcttccagtt gcaaacaggg ttcgccgcgc agttcacgca
    22081 ccgttctttc gagcatgaca ttaaaatgtt tacggataaa ccggatatct gtatccgcca
    22141 aatcgagaac ggttttgatc cccatcgcgt ccagtttttt gctgatccgc cgtccaatcc
    22201 cccagacgtc atccacgggg agagcagaca ttaatttacg ctggcgttcc agatttgata
    22261 aatccaccac cccacccgtc tgccgctgcc atttttttgc cgcatgattg gcaagcttag
    22321 ccaacgtttt ggtctgggcg atccccacac caacagtaag atgggtacgt tgtagcaccg
    22381 ttgcgcgaat ttctctgcca aaatcagtca gatcgcgaca attacgcaca cctgtcagat
    22441 cgcagaatgc ttcatcaata ctgtaaatct cgacgcgggg cgatagttct tccagcgtcg
    22501 acatcacccg attgctcatg tctgcgtaaa gctcataatt gctgctaaag caaaccacac
    22561 cacagcgacg aaacagatct ttttgtttga accagggatc gcccatttta acgcccagcg
    22621 cctttgcctc agcgtttcgg gcgataacgc aaccgtcatt attcgatagc acaaccaccg
    22681 gtttacccca taaatcaggg cgaaacaccg tctcacagct ggcataaaac gcgtttacat
    22741 cacagagggc aaacatcagc gcatcgcctt aacgacgtgg atcaccacac caaagacatc
    22801 cagcgtatct tcgctactga tggtaatggg cgagtacgca ctgttcatgg gaataagctg
    22861 taccgtcggg cgtagttgca attttttcac cgtaaactcg ccgtcaacag cagcgatgac
    22921 aatatcacca tggctggcgg taatagcact atcgacaatc agtaaatcac cgtaactaat
    22981 tccaccatca atcatagaat caccacttgc tttgacgaag taagtcgcgc tgggatgctg
    23041 gatcaacagt tgattcagat cgatgcgctg ttcaacgtaa tctgctgccg gtgaaggaaa
    23101 gccacactga acaagatcgc taaatagcgg aaaagtcaca atttcgcgga gatccgcagg
    23161 cttgataaac aacataataa tctgcctgaa gttatactgt ttttatatac agtggtctgt
    23221 tcttgccagc agatcaatac tgattcaggc tatcaatatt tgtcgctgca tagactgctg
    23281 atttttcgtt ctcttatcct gtgctcacgt ggccttctgg cgacgacgct catccagcag
    23341 aaatgaaaaa tatcacccgg caaaaaaata gaatagaagc attcgccata atg
//
LOCUS       DAZXUL010000061        22412 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZXUL010000061 DAZXUL010000000
VERSION     DAZXUL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.061"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            82..2682
                     /locus_tag="ACH3L1_002408"
     CDS             82..2682
                     /locus_tag="ACH3L1_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFT4077113.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3742
                     /locus_tag="ACH3L1_002409"
                     /pseudo
     CDS             2673..3742
                     /locus_tag="ACH3L1_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3754..4294
                     /locus_tag="ACH3L1_002410"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="ACH3L1_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="ACH3L1_002411"
     CDS             4302..4817
                     /locus_tag="ACH3L1_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4077114.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="ACH3L1_002412"
     CDS             4783..5520
                     /locus_tag="ACH3L1_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFT4077115.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="ACH3L1_002413"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="ACH3L1_002413"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFT4077116.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="ACH3L1_002414"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="ACH3L1_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFT4077117.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAI"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACH3L1_002415"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACH3L1_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFT4077118.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACH3L1_002416"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACH3L1_002416"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFT4077119.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="ACH3L1_002417"
     CDS             10827..12734
                     /locus_tag="ACH3L1_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFT4077120.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="ACH3L1_002418"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="ACH3L1_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFT4077121.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="ACH3L1_002419"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="ACH3L1_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFT4077122.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="ACH3L1_002420"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="ACH3L1_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFT4077123.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="ACH3L1_002421"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="ACH3L1_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFT4077124.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACH3L1_002422"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACH3L1_002422"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFT4077125.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="ACH3L1_002423"
     CDS             complement(17402..19162)
                     /locus_tag="ACH3L1_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFT4077126.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="ACH3L1_002424"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="ACH3L1_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFT4077127.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACH3L1_002425"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACH3L1_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFT4077128.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACH3L1_002426"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACH3L1_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFT4077129.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22412
                     /gene="sxy"
                     /locus_tag="ACH3L1_002427"
     CDS             22005..>22412
                     /gene="sxy"
                     /locus_tag="ACH3L1_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFT4077130.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5722 a         5257 c         5872 g         5561 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc aatctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagtaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tg
//
LOCUS       DAZXUL010000062        22348 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZXUL010000062 DAZXUL010000000
VERSION     DAZXUL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22348
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.062"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(2..298)
                     /locus_tag="ACH3L1_002428"
     CDS             complement(2..298)
                     /locus_tag="ACH3L1_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFT4077131.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="ACH3L1_002429"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="ACH3L1_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFT4077132.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACH3L1_002430"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACH3L1_002430"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFT4077133.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="ACH3L1_002431"
     CDS             complement(1784..2323)
                     /locus_tag="ACH3L1_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFT4077134.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="ACH3L1_002432"
     CDS             complement(2353..3096)
                     /locus_tag="ACH3L1_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFT4077135.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="ACH3L1_002433"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="ACH3L1_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3453..4091
                     /gene="ompW"
                     /locus_tag="ACH3L1_002434"
     CDS             3453..4091
                     /gene="ompW"
                     /locus_tag="ACH3L1_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFT4077136.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4151..4657)
                     /locus_tag="ACH3L1_002435"
     CDS             complement(4151..4657)
                     /locus_tag="ACH3L1_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFT4077137.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4703..5203)
                     /locus_tag="ACH3L1_002436"
     CDS             complement(4703..5203)
                     /locus_tag="ACH3L1_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFT4077138.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5289..5468)
                     /locus_tag="ACH3L1_002437"
     CDS             complement(5289..5468)
                     /locus_tag="ACH3L1_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFT4077139.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="ACH3L1_002438"
     CDS             complement(5848..6654)
                     /gene="trpA"
                     /locus_tag="ACH3L1_002438"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFT4077140.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="ACH3L1_002439"
     CDS             complement(6654..7847)
                     /gene="trpB"
                     /locus_tag="ACH3L1_002439"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFT4077141.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="ACH3L1_002440"
     CDS             complement(7859..9220)
                     /gene="trpCF"
                     /locus_tag="ACH3L1_002440"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFT4077142.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="ACH3L1_002441"
     CDS             complement(9221..10816)
                     /gene="trpD"
                     /locus_tag="ACH3L1_002441"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFT4077143.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="ACH3L1_002442"
     CDS             complement(10816..12378)
                     /gene="trpE"
                     /locus_tag="ACH3L1_002442"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFT4077144.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12400..12493)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="ACH3L1_002443"
     CDS             complement(12470..12514)
                     /gene="trpL"
                     /locus_tag="ACH3L1_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFT4077145.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12652..13533
                     /gene="rnm"
                     /locus_tag="ACH3L1_002444"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             12652..13533
                     /gene="rnm"
                     /locus_tag="ACH3L1_002444"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFT4077146.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13530..14150
                     /locus_tag="ACH3L1_002445"
     CDS             13530..14150
                     /locus_tag="ACH3L1_002445"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFT4077147.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14251..15123
                     /gene="rluB"
                     /locus_tag="ACH3L1_002446"
     CDS             14251..15123
                     /gene="rluB"
                     /locus_tag="ACH3L1_002446"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFT4077148.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="ACH3L1_002447"
     CDS             complement(15163..15753)
                     /gene="cobO"
                     /locus_tag="ACH3L1_002447"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFT4077149.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15750..16508)
                     /locus_tag="ACH3L1_002448"
     CDS             complement(15750..16508)
                     /locus_tag="ACH3L1_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFT4077150.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16728..17777
                     /gene="sohB"
                     /locus_tag="ACH3L1_002449"
     CDS             16728..17777
                     /gene="sohB"
                     /locus_tag="ACH3L1_002449"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFT4077151.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17813..18064)
                     /locus_tag="ACH3L1_002450"
     CDS             complement(17813..18064)
                     /locus_tag="ACH3L1_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFT4077152.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18444..21041
                     /gene="topA"
                     /locus_tag="ACH3L1_002451"
     CDS             18444..21041
                     /gene="topA"
                     /locus_tag="ACH3L1_002451"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFT4077153.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            21251..22225
                     /gene="cysB"
                     /locus_tag="ACH3L1_002452"
     CDS             21251..22225
                     /gene="cysB"
                     /locus_tag="ACH3L1_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFT4077154.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         5620 a         5533 c         5725 g         5470 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaagc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa
     3361 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     3421 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     3481 cagtaacaac tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     3541 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     3601 gtggattcag cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg
     3661 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     3721 cgaccggcga tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt
     3781 ttggtgatgc cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct
     3841 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     3901 aagattcctg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     3961 tggttaacat gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
     4021 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     4081 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     4141 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     4201 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     4261 tcgcttcgat gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     4321 aggtgtaaca ggtgatttca aattgctcga agacatatcc gccaatagag cctttaacta
     4381 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     4441 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     4501 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     4561 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     4621 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     4681 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     4741 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     4801 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     4861 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     4921 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     4981 atttcatgcg cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa
     5041 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     5101 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     5161 aaagcagttg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     5221 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     5281 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     5341 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     5401 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaact accacgatgt
     5461 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     5521 gacactcatt aaaattagtc actaatgaga attagtcaaa ttaagcgcaa cgagaagata
     5581 gagggaaata tattttgagg aacattctgg atatattaac aattacctga ggaatatgtg
     5641 acttagagaa aattattgat tttactggtg ttatgttgcg ggattaattt gtttgattat
     5701 attactgttg ggcggaaaaa tgacgtaagt tgacgttcga ccggggtaag cgaaacggta
     5761 aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa agttaaaatg
     5821 ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca tcggttgtac
     5881 aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct cgatgatttt
     5941 aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg cttttacctg
     6001 atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact ctttcagctt
     6061 cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca cgcccgctcg
     6121 tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagca ggtcgtcatc
     6181 ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg cctggcggaa
     6241 gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc cgactttctc
     6301 gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat acatcaacag
     6361 gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa aacattgtgc
     6421 cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg ggccatccgc
     6481 cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt caattagcgt
     6541 atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa
     6601 tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt ccatcagatt
     6661 tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa
     6721 aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt cagcgcatgg
     6781 gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca cagcgttttg
     6841 aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc agtgctgtta
     6901 agatacgcgt gttgtggacc gacagacggg aaatccagtc cggcggagat ggagtaagat
     6961 tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa ataaatgccc
     7021 acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg accaccaggc
     7081 tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat gccgatggca
     7141 ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc tctttccaga
     7201 atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat ggtcggataa
     7261 ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact gccggaccag
     7321 tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg taccgggatc
     7381 acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc aacgtctttg
     7441 gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc cgacgccacg
     7501 ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg cttcgccagc
     7561 aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa atcttcacgc
     7621 ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt cagtgcggtt
     7681 ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc aggatctttt
     7741 tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat ttgtggcacg
     7801 tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt
     7861 aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt
     7921 gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc
     7981 agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga ccatttaata
     8041 gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca tatttatcaa
     8101 cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc cagatggcaa
     8161 catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca ttgccatgca
     8221 gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca atatcgtgat
     8281 tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa
     8341 cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt
     8401 cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc tcacccagca
     8461 acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa ccaatcagaa
     8521 aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg ccggattcgc
     8581 tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa
     8641 tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct aatgcaatgg
     8701 cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc agactgtgag
     8761 cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt aataagcagg
     8821 catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg aagtctttac
     8881 ataaaatcgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag ctcccctgaa
     8941 aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa atggcggcaa
     9001 tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct tttttacact
     9061 ccagaataaa cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgt gtgctcggct
     9121 gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct acccaaatcg
     9181 ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt gccgccagcg
     9241 cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt tgcgcattgg
     9301 cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca gcgacggctg
     9361 cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca cggttttctt
     9421 caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa tcttcagcgg
     9481 tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc ggcgcgtgta
     9541 atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc
     9601 gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt aacgccagcg
     9661 gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg gttttcagtt
     9721 gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca aagaggaaac
     9781 atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca agattaatac
     9841 cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg ttaccgtgtt
     9901 tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa atattgatac
     9961 tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc gggcgcggga
    10021 acggcgctgc gttttccagt agcgcggttg ctgccccggc gatctcgttc gggtgctcac
    10081 cgcgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc tcgccacgca
    10141 ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc tgatacagtt
    10201 tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc caggccagcg
    10261 tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg aactggaatc
    10321 cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa tgggcgttga
    10381 tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc accggcagcg
    10441 ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac gctttaccgt
    10501 gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc
    10561 cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac
    10621 aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga ttgctcatcg
    10681 tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc accacgttat
    10741 gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata ttatcgagca
    10801 gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa tagcgcgcag
    10861 tacagcgcgg gctttgttac gggtttcgtc ggcttccgac tgcggaacag aatcaaggac
    10921 tacaccagca cccgcttgca cggtggcgat accgttttcc accagcgccg agcggatcac
    10981 aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc cgtagctgcc
    11041 gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg gcgcaccgct
    11101 taacgtcccc atattcatac aggcgcgata agcgtgcagg gcgtcaagat cgtgacgcag
    11161 ttcgccgact acgcgagaga cgaggtgcat cacataggaa taacggtcaa ctttggtgag
    11221 atcggcgatg tagcggctgc caggggtgca aatgcgtgcc agatcattac gggcgagatc
    11281 aaccagcatc agatgttcag acagctcttt atggtcagta cgcatttcca gttcaatacg
    11341 gctgtcgaga tctctgtcca gtgaaccatc ggcgcgacga ccgcgtgggc gtgttccggc
    11401 aatcgggtag atctcaatct ggcggctggt ggcatcatac ttgagcgagc tttccggcga
    11461 cgcgccaaat agggtgaaat cattatcctg cataaaaaac atgtacgggc tgggattact
    11521 ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga aacggcgaga
    11581 tggcaccacc tggaaaattt ctccagcgcg aatcgctttt tgcaacaaac gcactacgcc
    11641 accgaactct tcatcgctct gattacattc acaacgcata tgcggcacgg aaaccactgg
    11701 cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag cagtgagacg
    11761 ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc tttttttctg
    11821 atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc agttattttc
    11881 cgctgacagt tgcggtaaat cttcaaatcc cgccacaagg tcataagaga acaggccgcc
    11941 gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgca ataaacggaa
    12001 agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg gactgacagg
    12061 ggggaagcgc agcacacggc agtttggtga ttgttcactt tccacacccg caggcagggc
    12121 gttatccagt agtgccagca gggcttcgcc attgccggaa agtgcctgaa ttgtgacggt
    12181 gtcacctaat gctgtaatgc gcagcgcact gtctatcagc agcaggcttt ttaaatcatc
    12241 tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgattcc cacacaactg
    12301 gtgaaaaagc gcagtcgggt tgtcgcgata agcgccttcg caggttagca gttcgagagt
    12361 cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc cgctcattag
    12421 gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt caggaagtgc
    12481 gccaccaacc tttcagtacg aaaattgctt tcattgtcga tacccttttt acgtgaactt
    12541 gcgtactagt taactggttc gatgattaat tgtcaacagc tcatttcaga atatttgcca
    12601 gacccgttat gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac
    12661 acgaattatg cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg
    12721 acgccagaag cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc
    12781 gaccatgaca ccacagctgc aatcgcgcca gcaagagagg aaatttcacg ttcgggactg
    12841 gcgctgaatc ttattcccgg cgtggaaatt tccacggtct gggaaaatca tgaaattcat
    12901 attgtcgggc tgaatattga tattactcat ccgctaatgt gtgagttcct cgcgcagcag
    12961 acagaacggc gcaatcagcg ggcgcagcaa attgccgaaa gacttgaaaa agcgcaaata
    13021 cctggggcgc tggaaggcgc acaacgactg gcgcagggcg gcgcagtgac gcgcggtcat
    13081 tttgcgcgtt tccttgtgga gtgcggcaaa gccagttcaa tggcagatgt ctttaaaaag
    13141 tatctggcgc gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat agaacaagct
    13201 attgatgtca ttcatcattc tggcggtaag gcggtgctag ctcatcctgg gcggtacaat
    13261 cttagtgcta aatggctgaa aagattggtt gcgcattttg ccgaacacca cggcgacgcg
    13321 atggaagtcg cgcagtgcca gcaatcgccc aatgaacgca cccagctggc cacacttgcg
    13381 cgtcagcatc atttatgggc atcacaagga tctgattttc atcagccatg cccgtggatc
    13441 gaactgggtc gtaaactctg gctgcccgca ggcgttgaag gcgtctggca gctatgggaa
    13501 cagccgcaga acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata
    13561 acccgcagca acgtctgatc aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg
    13621 tctatccaac tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgccatgg
    13681 agcgtatttg tcgtattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg
    13741 atctttctga actctcgacc tattcattcg tggataacgt tgcgtttcgt ttgatgaaaa
    13801 acaacacgcc gggcaactat accttcatcc tgaaggggac gaaggaagtg ccacgccgtc
    13861 tgttgcagga gaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg attgcgcagg
    13921 cgttgcttga agcactgggc gaaccgatgc tttccacttc gctaatgctg ccaggcagcg
    13981 aatttaccga atccgatccc gaagaaatta aagaccgtct ggaaaaacag gtagatttga
    14041 ttattcacgg cggctatctc gggcagaaac caacaacggt tattgatctt accgatgata
    14101 cgccggtcgt ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa gtgcgtaact
    14161 ctgtatacta cgcggcctta aaatggcgct atacggcgcc attgattctc ctgttcgacg
    14221 cctgtgaagg cgacaccaaa aggaagctct atgagcgaaa aattacagaa agtgctggcc
    14281 cgcgctggcc acggctcccg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt
    14341 gttgatggca aaattgctaa actcggcgat cgcgttgaag ttacccctgg gctgaaaatt
    14401 cgtatcgatg gtcatctgat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg
    14461 gcctattaca aaccggaagg tgagttgtgt acccgtaacg atccggaagg gcgtccgacg
    14521 gtgtttgacc gtctgccaaa gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac
    14581 gttaacacct gtggtctgct gctgttcacc accgatggtg aactggcaaa ccgtttaatg
    14641 catccaagcc gtgaagttga acgtgaatac gccgtgcgtg tatttggtca ggttgacgac
    14701 gcgaaactgc gtgatttgag tcgtggcgtg caactggaag atggtccggc agcttttaaa
    14761 accatcaagt tcagcggcgg cgaagggatc aaccagtggt acaacgtgac tctgaccgaa
    14821 ggccgtaacc gtgaagttcg tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg
    14881 atccgtgttc gttacggtga tatcccactg ccaaaaggtc tgccacgcgg tggctggacg
    14941 gagctggacc tcgcccagac taactatctg cgcgaactgg tggagcttcc gccagaaacc
    15001 agctctaaag tcgctgtaga aaaagaccgt cgtcgcatga aggctaatca gattcgtcgt
    15061 gcggtgaaac gtcacagtca ggtgagcggc agtcgtcgtt ctggcgggcg taataacggt
    15121 taatcaaagt atgccggacg tcatatccgg catttttaca gattaataat caatccctat
    15181 ttgcgcctta acaccggcat caaacgcatg tctgatgggg cgcagctcac ttaccgtatc
    15241 cgccagttca agaatatccc gatgacagcc tcgacccgtg ataatcaccg tctgttgatg
    15301 tggacgttca tttaacgcct gcaccacttc ttccagtggc aaatagtcat acgccaccat
    15361 atacgtcagt tcatcaagca aaaccatatc cagtgaggaa tcagcaagca tccgctttgc
    15421 atgttgccag acttcgcggc aggcggcggt atcagactcg cggttttgtg tatcccaggt
    15481 aaagcccgtt gccatcacct gaaactcaac gccatgtggc tccagcagat tgcgttcgcc
    15541 attaggccag gtgcctttaa taaactgcac gacgcccact ttttttccgt gaccaactgc
    15601 gcgtgttgcc gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg taaagacgat
    15661 gataataccg cgttcatcct gggcctgggc cacacgagca tctacttttt ctttcactcg
    15721 ctgctgacgc tgttggtagc gttcatcact cattgggaaa ttcctggttt acggcccggt
    15781 tgggcgtcaa aggtcatgcc ggttttacgg cggctgtcat cgcccatcag ccagaggtag
    15841 agcggcatga tatcagcggg tgttttaagt ttctgtggat cttcggtcgg gaaggcgctt
    15901 gcgcgcattg cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag gcgctgctga
    15961 tattcatcgg ccagtacctg catcatccct tcggtggcaa atttcgacgc tgcatatgca
    16021 ccccagttgg ctcgtccctg acgtccaacg cttgatgaag taaagaccag tgaaccggcg
    16081 tccgatttga gtaataaagg aagcagtgcc tgggtgagca taaaggtggc attaacgttg
    16141 atctgcatga cgtcctgcca gacctgcgga ttttgttcgc tcattgggca aacatcgccg
    16201 agcaatccgg cattatgcaa aacaccatcc agacgcggat aattaacgac aatgcgctgt
    16261 gccagttgtt ggcaattttc ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc
    16321 tgacgcccag tttcttcgtt tatgtggctg gctacctgac gtaatttttc ttcattacgg
    16381 cccaacagaa tcactgtcgc accatagcgt gcatacgtca tcgcggcttc acgaccaatc
    16441 ccatcgctgg ctcccgtcac caggataatg cgatcattga gtaaatcttg ttttggctgg
    16501 taatgcatgg ctactcctca acgacgttgt ctgtcgcgct gccagtgtac tttatgactt
    16561 tggggcttta tgcctgaaac agaagggcat ttcaatcagc ctggggcgat aacaccgcaa
    16621 aatggatact ttgtcatact ttcgctgcaa taacatctct gcgagacggc ttaacatgcc
    16681 tgttgtaaac tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg gaattgttgt
    16741 ctgaatatgg tttgtttttg gctaaaatcg ttaccgttgt gctagcgatt gcggcgattg
    16801 ccgccattat tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag ttacgggtca
    16861 acaatctcag cgaacagtat aaggagatga aagaagaact ggccgcggcg ctgatggact
    16921 cacatcagca aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag
    16981 cagcaaaagc gaaagccaaa ctgggcgagg tggcaactga cagtaaacct cgcgtctggg
    17041 tgctggattt taaaggcagc atggacgccc atgaagtgaa ctcgctacgt gaagagataa
    17101 cggcggtact cgcagcattc aaaccgcagg atcaggttgt gctccgtctg gaaagccctg
    17161 gtggcatggt gcatggttac gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa
    17221 acattccttt aactgttacg gtagacaaag tcgctgccag cggcggttac atgatggcct
    17281 gtgtggcgga caaaattgtt tccgcaccgt ttgctattgt gggttccatt ggggtggtgg
    17341 cgcaaatgcc caactttaac cgcttcctga aaagcaaaga tattgatatc gaactgcaca
    17401 ccgccgggca gtataagcgc acgctgacct tgctgggtga aaataccgaa gaagggcggg
    17461 agaaattccg cgaagagctg aacgaaacgc atcagttatt taaagatttt gtgaagcgta
    17521 tgcgtccgtc tctggatatt gaacaggtgg caacgggtga acactggtac ggacaacagg
    17581 cggtagagaa aggcctggtt gatgaaatca acaccagtga tgaagttatt cttagcctga
    17641 tggaaggccg tgaagtggtt aatgtacgct atatgcagcg taaacgactc attgaccgat
    17701 tcaccggcag cgcggcagag agcgccgatc gattgttgct acgctggtgg cagcggggtc
    17761 aaaagccatt gatgtaaaag acaaacgcga ggctaagacc tcgcgttttg ctttaatcaa
    17821 ccagatgata tttttctgaa agcacatggg ccaggtgttt gaacatatta aacaccgcgg
    17881 tgcttttggc tgttggcaat ccttgttcat ctaaaaagta gtcgccggta aataccagca
    17941 cgccattacg ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc tcatgctcac
    18001 gaatgatttt gttggcctct ttcaacagcg tttcgcggtc gatgggttgt gtctctttgt
    18061 tcattattta ctccttaaac aaggacatta gtctacgcca ggcatggctt gcagacaaat
    18121 ataccacgct ggtggcaaga gcgccttact ggcaactttg gattttgcat gctaataaag
    18181 ttgcgtatcg gattttatca ggtacagtgt gacgctttcg tcaatctggc aatagatttg
    18241 cttgacattc gaccaaaaat ccgtcgtgct atagcgcctg taggccaaga cctgttaact
    18301 cagtcacctg aattttcgtg aacagagtca cgacaagggg ttgatatccg cagagagcga
    18361 gtccatatcg gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa
    18421 gaatcaaatt aggtaaggtg aatatgggta aagctcttgt catcgttgag tccccggcaa
    18481 aagccaaaac gatcaacaag tatctgggtt gtgactacgt ggtgaaatcc agcgtcggtc
    18541 acatccgcga tttgccgacc agtggctcag ctgccaaaaa gagtgccgac tctacctcca
    18601 ccaagacggc taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg
    18661 ttgacccgtg gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg
    18721 tctctgaact gaaacaactg gctgaaaaag ccgaccacat ctatctcgca accgaccttg
    18781 accgcgaagg ggaagccatt gcatggcacc tgcgggaagt gattggtggt gatgatgcgc
    18841 gctatagccg agtggtgttt aacgaaatta ctaaaaacgc gatccgccag gcatttaaca
    18901 aaccgggtga gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc
    18961 gcgtggtggg gtatatggtt tcgccgctgc tgtggaaaaa gatcgctcgt ggtctgtctg
    19021 ccggtcgtgt gcagtcggtg gcggtccgcc tggtggtaga gcgtgagcgt gaaattaaag
    19081 cgttcgtacc ggaagagttc tgggaagtcg atgccagcac gactacgcca tctggtgaag
    19141 cgttggagtt gcaggtgact catcagaatg acaaaccgtt ccgtccggtc aacaaagaac
    19201 aaactcaggc tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag
    19261 acaaaccgac aaccagtaaa cctggcgctc cttttattac ctctacgctg caacaagctg
    19321 ccagcacccg tcttggtttt ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg
    19381 aagcaggcta tatcacttac atgcgtaccg actccactaa cctgagtcag gacgcggtaa
    19441 atatggttcg cggttatatc agcgataatt ttggtaagaa atatctgccg gaaagtccga
    19501 atcagtacgc cagcaaagaa aactcacagg aagcgcacga agcgattcgt ccttctgacg
    19561 tcaatgtgat ggcggaatcg ctgaaggata tggaagcaga tgcgcagaaa ctgtaccagt
    19621 taatctggcg tcagttcgtt gcctgccaga tgaccccagc gaaatatgac tccacgacgc
    19681 tgaccgttgg tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg cgctttgatg
    19741 gctggacaaa agtgatgcct gcactgcgta aaggcgatga agatcgtatc ttaccggcag
    19801 tcgataaagg cgatgctctg acgctcgttg aactaacacc agcccagcac tttaccaagc
    19861 cgccagcccg tttcagtgaa gcatcgctgg ttaaagaact ggaaaaacgt ggtatcggtc
    19921 gtccgtctac ctatgcgtcg atcatttcga ccattcagga tcgtggctat gtgcgagtag
    19981 aaaatcgtcg tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa
    20041 atttccgcga gttaatgaac tacgacttta ccgcgcagat ggaaaacagc ctcgaccagg
    20101 tggcaaatca cgaagcagag tggaaagctg tactggatca cttcttctcg gatttcaccc
    20161 agcagttaga taaagctgaa aaagatccgg aagagggtgg tatgcgcccg aaccagatgg
    20221 ttctgaccag cattgactgc ccgacttgtg gtcgcaaaat ggggattcgc acagcgagta
    20281 ccggggtatt ccttggctgt tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca
    20341 ccattaacct ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa
    20401 ccaacgcgct gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc
    20461 tcatcgatcc gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc gacggttacg
    20521 agatcgaaga gggcgaattc cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa
    20581 aatgtggctc tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacca
    20641 acgaagagtg taaaaacaca cgtaagattt tacgtaacgg tgaagtggca ccaccgaaag
    20701 aagatccggt gccattacct gagctgccgt gcgaaaaatc agatgcttat ttcgtgctgc
    20761 gtgacggtgc tgccggtgtg ttcctggctg ccaacacttt cccgaaatcg cgtgaaacgc
    20821 gtgcgccact ggtggaagag ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt
    20881 atctggccga tgcgccacag caggatccgg aaggtaataa gaccatggtt cgctttagcc
    20941 gtaaaaccaa acagcaatat gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag
    21001 cattttatgt tgatggcaaa tgggttgaag gaaaaaaata acctttaatt ctgtcaggtt
    21061 tttataaaca aagggtcgcg aaagcggccc ttttttattg catattattt tttcttcaca
    21121 cctatacact aaggctataa atgatatagt ggttatagtt agcacctttt ttattattaa
    21181 atcgtattag tcacccgcca ggtgtgacga aaaaacgatg ttctgatggc gtctaagtgg
    21241 atggtttaac atgaaattac aacaacttcg ctatattgtt gaggtggtca atcataacct
    21301 gaatgtctca tcaacagcgg aaggacttta cacatcacaa cccgggatca gtaaacaagt
    21361 cagaatgctg gaagacgagc taggcattca aattttttcc cgaagcggca agcacctgac
    21421 gcaggtaacg ccagcaggac aagaaataat tcgtatcgct cgcgaagtcc tgtcgaaagt
    21481 cgatgccata aaatcggttg ccggtgagca cacctggccg gataaaggct cgctgtatat
    21541 cgccaccacg catacccagg cacgctacgc gttaccaaac gttatcaaag gctttattga
    21601 gcgttatccc cgcgtttctt tgcatatgca ccagggcccg ccgacacaaa ttgctgatgc
    21661 cgtctctaaa ggcaatgccg atttcgctat cgccacagaa gcgctgcatc tgtatgaaga
    21721 tttagtgatg ttaccgtgct accactggaa tcgggctatt gtagtcactc cggatcaccc
    21781 gctggcaggc aaaaaagcca ttaccattga agaactggcg caatatccgt tggtgacata
    21841 taccttcggc tttaccggac gttcagaact ggatactgcc tttaaccgcg cagggttaac
    21901 gccgcgtatc gttttcacgg caacggatgc tgacgtcatt aaaacttacg tccggttagg
    21961 gctgggggta ggggtcattg ccagcatggc ggtggatccg gtcgccgatc ccgaccttgt
    22021 gcgcgttgat gctcacgata tcttcagcca cagtacaacc aaaattggtt ttcgccgtag
    22081 tacttttttg cgcagttata tgtatgattt cattcagcgt tttgcaccgc atttaacgcg
    22141 tgatgtcgtt gatgcggctg tcgcattgcg ctccaatgaa gaaattgagg tcatgtttaa
    22201 agatataaaa ctgccggaaa aataatttct tgcgttattt tcggcacctt ttatgtagcg
    22261 aaggtgccgg aatatattct cttttgttac ttatttctat accgtgcaca atatttttat
    22321 ccctcatatt tatagggtaa attacctg
//
LOCUS       DAZXUL010000063        21508 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZXUL010000063 DAZXUL010000000
VERSION     DAZXUL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21508
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.063"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACH3L1_002453"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACH3L1_002453"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFT4077155.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACH3L1_002454"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACH3L1_002454"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFT4077156.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="ACH3L1_002455"
     CDS             complement(3121..3420)
                     /locus_tag="ACH3L1_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFT4077157.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="ACH3L1_002456"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="ACH3L1_002456"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFT4077158.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACH3L1_002457"
     CDS             complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACH3L1_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFT4077159.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACH3L1_002458"
     CDS             complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACH3L1_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFT4077160.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACH3L1_002459"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACH3L1_002459"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFT4077161.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACH3L1_002460"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACH3L1_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="ACH3L1_002461"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="ACH3L1_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFT4077162.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="ACH3L1_002462"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="ACH3L1_002462"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFT4077163.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="ACH3L1_002463"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="ACH3L1_002463"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFT4077164.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13865
                     /gene="xylR"
                     /locus_tag="ACH3L1_002464"
                     /pseudo
     CDS             12687..13865
                     /gene="xylR"
                     /locus_tag="ACH3L1_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(13973..14797)
                     /locus_tag="ACH3L1_002465"
     CDS             complement(13973..14797)
                     /locus_tag="ACH3L1_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFT4077165.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="ACH3L1_002466"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="ACH3L1_002466"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFT4077166.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="ACH3L1_002467"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="ACH3L1_002467"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFT4077167.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="ACH3L1_002468"
     CDS             complement(18729..19202)
                     /locus_tag="ACH3L1_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFT4077168.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="ACH3L1_002469"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="ACH3L1_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFT4077169.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="ACH3L1_002470"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="ACH3L1_002470"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFT4077170.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21508
                     /locus_tag="ACH3L1_002471"
     CDS             21362..>21508
                     /locus_tag="ACH3L1_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFT4077171.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQI"
BASE COUNT         5405 a         5490 c         5277 g         5336 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga tgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcattga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgcaaatt
//
LOCUS       DAZXUL010000064        21350 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZXUL010000064 DAZXUL010000000
VERSION     DAZXUL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21350
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.064"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACH3L1_002472"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACH3L1_002472"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFT4077172.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="ACH3L1_002473"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="ACH3L1_002473"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFT4077173.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACH3L1_002474"
     CDS             complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACH3L1_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFT4077174.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="ACH3L1_002475"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="ACH3L1_002475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFT4077175.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="ACH3L1_002476"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="ACH3L1_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACH3L1_002477"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACH3L1_002477"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFT4077176.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACH3L1_002478"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACH3L1_002478"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFT4077177.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACH3L1_002479"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACH3L1_002479"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFT4077178.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACH3L1_002480"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACH3L1_002480"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFT4077179.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACH3L1_002481"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACH3L1_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFT4077180.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="ACH3L1_002482"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="ACH3L1_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFT4077181.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="ACH3L1_002483"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="ACH3L1_002483"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFT4077182.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNLGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACH3L1_002484"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACH3L1_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFT4077183.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACH3L1_002485"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACH3L1_002485"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFT4077184.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACH3L1_002486"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACH3L1_002486"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFT4077185.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACH3L1_002487"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACH3L1_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFT4077186.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="ACH3L1_002488"
     CDS             complement(18170..18565)
                     /locus_tag="ACH3L1_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFT4077187.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACH3L1_002489"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACH3L1_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFT4077188.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="ACH3L1_002490"
     CDS             19481..20074
                     /locus_tag="ACH3L1_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFT4077189.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="ACH3L1_002491"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="ACH3L1_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFT4077190.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21350
                     /gene="yjgL"
                     /locus_tag="ACH3L1_002492"
     CDS             20794..>21350
                     /gene="yjgL"
                     /locus_tag="ACH3L1_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFT4077191.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARL"
BASE COUNT         5130 a         5308 c         5636 g         5276 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaacct cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt
//
LOCUS       DAZXUL010000065        21276 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZXUL010000065 DAZXUL010000000
VERSION     DAZXUL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21276
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.065"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..444)
                     /locus_tag="ACH3L1_002493"
     CDS             complement(<1..444)
                     /locus_tag="ACH3L1_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFT4077192.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /gene="manZ"
                     /locus_tag="ACH3L1_002494"
     CDS             complement(499..1350)
                     /gene="manZ"
                     /locus_tag="ACH3L1_002494"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFT4077193.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /gene="manY"
                     /locus_tag="ACH3L1_002495"
     CDS             complement(1363..2163)
                     /gene="manY"
                     /locus_tag="ACH3L1_002495"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFT4077194.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="ACH3L1_002496"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="ACH3L1_002496"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFT4077195.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3514..3672
                     /gene="yoaL"
                     /locus_tag="ACH3L1_002497"
     CDS             3514..3672
                     /gene="yoaL"
                     /locus_tag="ACH3L1_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFT4077196.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="ACH3L1_002498"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="ACH3L1_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFT4077197.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="ACH3L1_002499"
     CDS             complement(5214..6812)
                     /locus_tag="ACH3L1_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFT4077198.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="ACH3L1_002500"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="ACH3L1_002500"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFT4077199.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="ACH3L1_002501"
     CDS             complement(8491..9069)
                     /locus_tag="ACH3L1_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFT4077200.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="ACH3L1_002502"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="ACH3L1_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFT4077201.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="ACH3L1_002503"
     CDS             10508..10687
                     /locus_tag="ACH3L1_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFT4077202.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11106)
                     /locus_tag="ACH3L1_002504"
     CDS             complement(10807..11106)
                     /locus_tag="ACH3L1_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFT4077203.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="ACH3L1_002505"
     CDS             complement(11528..11872)
                     /locus_tag="ACH3L1_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFT4077204.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="ACH3L1_002506"
     CDS             12004..13914
                     /locus_tag="ACH3L1_002506"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFT4077205.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="ACH3L1_002507"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="ACH3L1_002507"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFT4077206.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="ACH3L1_002508"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="ACH3L1_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFT4077207.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15494..17179
                     /gene="fadD"
                     /locus_tag="ACH3L1_002509"
     CDS             15494..17179
                     /gene="fadD"
                     /locus_tag="ACH3L1_002509"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFT4077208.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17261..18376
                     /gene="rnd"
                     /locus_tag="ACH3L1_002510"
     CDS             17261..18376
                     /gene="rnd"
                     /locus_tag="ACH3L1_002510"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFT4077209.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="ACH3L1_002511"
     CDS             complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="ACH3L1_002511"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFT4077210.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="ACH3L1_002512"
                     /pseudo
     CDS             complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="ACH3L1_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(20410..21018)
                     /locus_tag="ACH3L1_002513"
     CDS             complement(20410..21018)
                     /locus_tag="ACH3L1_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4077211.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21188..>21276
                     /locus_tag="ACH3L1_002514"
     CDS             21188..>21276
                     /locus_tag="ACH3L1_002514"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4077212.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5266 a         5273 c         5465 g         5272 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta cgacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcccc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatctttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcagggggtc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgagtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgatg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtac cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaatgca gaaacagcgg ctggtccgct gtttctgcat tcttacggta aagataaaaa
    15361 taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg tatgatgagt
    15421 taatattatg ttaacggcgt gtatatcatt tggggttgcg atgacgacga acacgcattt
    15481 tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg ttccgacgga
    15541 gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg tcgcgcgcta
    15601 cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca agctggaaga
    15661 acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga aaggcgatcg
    15721 cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg gcattttgcg
    15781 tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc ttgagcatca
    15841 gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc acactctgga
    15901 aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg gcgatcagct
    15961 atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc gcttggtgcc
    16021 gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg gctaccggat
    16081 gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat acaccggcgg
    16141 caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg cgaacctgga
    16201 acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg tagtaacggc
    16261 actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta tcgaactggg
    16321 tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa aagagttagc
    16381 gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt tgctgaacaa
    16441 taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg gtgggatgcc
    16501 tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc tgctggaagg
    16561 ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata ttgattatca
    16621 tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg atgacgacga
    16681 caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc aggtgatgct
    16741 gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct ggttacacac
    16801 cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc gtaaaaaaga
    16861 catgattttg gtttccggtt ttaacgttta tcccaacgag attgaagatg tcgtcatgca
    16921 gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca gtggtgaagc
    16981 ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac tggtgacttt
    17041 ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc gtgatgagtt
    17101 accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag cgcgcggcaa
    17161 agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat ccggcgtttt
    17221 ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca cggacgatgc
    17281 gctggcttct ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg atactgaatt
    17341 tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg atggcgagca
    17401 tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag cgatcctgcg
    17461 cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag tgttcctcaa
    17521 tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg ccttctgcgg
    17581 acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg ttacgctgga
    17641 caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc agtgtgaata
    17701 cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg tagaaacgga
    17761 ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa tgcgtcgtca
    17821 ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc agttacgcac
    17881 acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg cgcgagagcg
    17941 tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg cgcgttatat
    18001 gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa tccgctttca
    18061 cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag atgccttacc
    18121 gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagcgttta aagcgattaa
    18181 gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc tggcatcgcg
    18241 tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga acaatttgcc
    18301 ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca atttattgca
    18361 ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc cacatccggc
    18421 tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac aacagatcaa
    18481 catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat ggtcagcttc
    18541 accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata aacattccac
    18601 tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg gcaccacaaa
    18661 ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt cttcgatagc
    18721 aaattgctca aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt cacttcttac
    18781 cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt cgatatccgc
    18841 caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat ggactttctc
    18901 agcctgcgga tgctggacta gctcagcacg aaatgcgcaa ctttctggat ttcgcgagca
    18961 gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg acaggaaagg
    19021 ggtgatacca ataccgccca cgataaacag atgctttctg gctgagggaa ttagcgcaaa
    19081 caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct gatgcaaaaa
    19141 gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca atctgataac tagaggtgtt
    19201 atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat cgctcatctg
    19261 cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg ttgccaccag
    19321 cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta cttcaaacat
    19381 ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga aaataccgcc
    19441 gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg gcgataccat
    19501 gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca cgatagccac
    19561 gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga tggaacacat
    19621 cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg gtgaagtaaa
    19681 tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg accgtcatca
    19741 tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa ccgtggaatg
    19801 ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg aaaccgtatt
    19861 gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg gagtcggaga
    19921 aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca atgttcttcc
    19981 agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg acttccgggc
    20041 aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt acgaaaaaaa
    20101 accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg ggcttgcaag
    20161 cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa aaaattgctt
    20221 ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat catttcaata acagcttcaa
    20281 aaaacgttca aaaccgataa caaccaggct gtaaccaaat gactcatatc acaaatcagc
    20341 tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt tttagctggt
    20401 tcatggttgt tatacggctt tttttacatc ttgtggttct tgtgaaggag ctacaagacc
    20461 ttcctgttca taaataccat actttgacaa aatcttttta cgagtatctg ttggtaatga
    20521 ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata ccttgcaaat cagcctggat
    20581 ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca aatcctttct ttctgaacaa
    20641 ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg acaattgcga tcagtttagt
    20701 tttcaatgtg ctgcggtcag gttgagaatt tttattctct tgattatttt tatgctcaac
    20761 cattggcatg ctctgacttt ggataaacca ctctattgga cgccctgttg cgtaggctat
    20821 ttgtgctaat cgatctaaag acgggtatgt cttgccagac aggtagtttc tgataacccc
    20881 atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat tctccattca ttgcctcttc
    20941 aaggcgttcc ttgaaacgca ttatttccgg gggcataaaa atgcgctctg catttttgct
    21001 ttctttttca cctgtcatgc ccaatctctc tgttatataa agaattttac agaattcttt
    21061 gatttgcact ggttaaaata aaaatgcgca tagcttttga attgagcatt tttctgcgct
    21121 atcgtttttc ccgatagcgg tagccggtat cagttaccga aaacgttaag agagtaagga
    21181 tcgcagaatg tcagcaaaaa accaagactg gcacgcagag cagataaagg ctgctgttcg
    21241 tatgaagggg aagacactat ctcaattatc cagaga
//
LOCUS       DAZXUL010000066        21256 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZXUL010000066 DAZXUL010000000
VERSION     DAZXUL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21256
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.066"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..794
                     /locus_tag="ACH3L1_002515"
                     /pseudo
     CDS             1..794
                     /locus_tag="ACH3L1_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(854..2152)
                     /gene="eno"
                     /locus_tag="ACH3L1_002516"
     CDS             complement(854..2152)
                     /gene="eno"
                     /locus_tag="ACH3L1_002516"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFT4077213.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2240..3877)
                     /gene="pyrG"
                     /locus_tag="ACH3L1_002517"
     CDS             complement(2240..3877)
                     /gene="pyrG"
                     /locus_tag="ACH3L1_002517"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFT4077214.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4105..4896)
                     /gene="mazG"
                     /locus_tag="ACH3L1_002518"
     CDS             complement(4105..4896)
                     /gene="mazG"
                     /locus_tag="ACH3L1_002518"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFT4077215.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4992..7226)
                     /gene="relA"
                     /locus_tag="ACH3L1_002519"
     CDS             complement(4992..7226)
                     /gene="relA"
                     /locus_tag="ACH3L1_002519"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFT4077216.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7274..8575)
                     /gene="rlmD"
                     /locus_tag="ACH3L1_002520"
     CDS             complement(7274..8575)
                     /gene="rlmD"
                     /locus_tag="ACH3L1_002520"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFT4077217.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8632..11388
                     /gene="barA"
                     /locus_tag="ACH3L1_002521"
     CDS             8632..11388
                     /gene="barA"
                     /locus_tag="ACH3L1_002521"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFT4077218.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11620..12960)
                     /gene="gudD"
                     /locus_tag="ACH3L1_002522"
     CDS             complement(11620..12960)
                     /gene="gudD"
                     /locus_tag="ACH3L1_002522"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFT4077219.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12981..14321)
                     /locus_tag="ACH3L1_002523"
     CDS             complement(12981..14321)
                     /locus_tag="ACH3L1_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFT4077220.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14323..15675)
                     /gene="gudP"
                     /locus_tag="ACH3L1_002524"
     CDS             complement(14323..15675)
                     /gene="gudP"
                     /locus_tag="ACH3L1_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFT4077221.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16109..16558)
                     /locus_tag="ACH3L1_002525"
     CDS             complement(16109..16558)
                     /locus_tag="ACH3L1_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFT4077222.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16576..17358)
                     /gene="truC"
                     /locus_tag="ACH3L1_002526"
     CDS             complement(16576..17358)
                     /gene="truC"
                     /locus_tag="ACH3L1_002526"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFT4077223.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17358..17687)
                     /locus_tag="ACH3L1_002527"
     CDS             complement(17358..17687)
                     /locus_tag="ACH3L1_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFT4077224.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18309..18854)
                     /gene="syd"
                     /locus_tag="ACH3L1_002528"
     CDS             complement(18309..18854)
                     /gene="syd"
                     /locus_tag="ACH3L1_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFT4077225.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18922..19770
                     /gene="queF"
                     /locus_tag="ACH3L1_002529"
     CDS             18922..19770
                     /gene="queF"
                     /locus_tag="ACH3L1_002529"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFT4077226.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19882..21246
                     /gene="ppnN"
                     /locus_tag="ACH3L1_002530"
     CDS             19882..21246
                     /gene="ppnN"
                     /locus_tag="ACH3L1_002530"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFT4077227.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5228 a         5671 c         5068 g         5289 t
ORIGIN      
        1 ttgttgcagc ccaaccaact attgtcccac aattacagca acaagttacg ggtcttacga
       61 gtagcttaaa ctcacaagaa aagaaagaac tgacgcacaa gttagaatct attttcaata
      121 atacccaagt gcaaattgct gtattaattg tttccacaac caaagatgag acaattgaac
      181 aatatgctac aagagttttt gacaattggc gtttaggaga tgccaaacgt aatgatggga
      241 tactgatcgt tgttgcctag tcggatcgca ctgtccgcat ccaggtaggt tatgggctgg
      301 aagaaaaggt aaccgatgct ctggctggag atatcatccg tagcaacatg atacccgcat
      361 ttaaacaaca aaaattagct aagggattag agttagctat aatcgctttg aataaccaac
      421 tcacttcaca acaccaatat ccggctaatc cttcagaaag tgaatcaacg tcttccactg
      481 atcattatta ctttgctatt ttttgggtat ttgcagtgat gttcttccct ttctggtttt
      541 ttcatcaagg cagtaatttt tgtcgcgcat gtaaaagtgg agtctgtatt tcagcaatcc
      601 atcttttaga tttattcctg ttctccgata aaactttttc cattgctgta ttttccttct
      661 tttttacttt caccatattt atggtcttta cctgtttatg tgtacttcag aaaagagcat
      721 ctggtagaag ttatcattca gacaacagcg gttccgctgg aggctcaagt ggcggcgcat
      781 ccggccgctg gtaactccac cataaaaaat gccagcccga aggctggcat tatcaaatca
      841 gataaagtca gacttatgcc tggcctttga tctctttacg accgttgtac ggtgcttttt
      901 cgcccagagc ttcttcgata cgaatcagct ggttgtattt agcaacacgg tcagaacggc
      961 tcatagaacc agttttgatc tggcctgcag cagtaccaac agccagatca gcgatggtag
     1021 catcttcagt ttcaccagaa cggtgagaga taactgcagt gtagccagca tctttcgcca
     1081 tcttgattgc agccagagtt tcggtcagag aaccgatctg gttgaatttg atcaggatgg
     1141 agttagcgat acctttttcg ataccttctt tcaggatctt ggtgttggtt acgaacaggt
     1201 cgtcaccaac cagctggatt ttgtcgccca gaactttggt ctggtatgcg aaaccgtccc
     1261 agtcagattc gtccagaccg tcttcgatag aaacgatcgg gtactgtttg gtcagttctt
     1321 ccaggaagtg agtgaattct tcagaggtga acgctttgtt gccttcgcca gccagaacgt
     1381 atttaccatc tttgtagaat tcagaagctg cgcagtccat cgccagagtg atgtctttgc
     1441 ccagttcata accagcagct ttaacagctt cagcgataac agccagagct tcagcgttgg
     1501 aacccaggtt cggcgcatag ccaccttcgt caccaacagc agtgttcatg cctttcgctt
     1561 tcagaacttt tgccaggtga tggaaaactt cagaacccat gcggatggct tctttcacag
     1621 ttttcgcgcc aaccggctga atcatgaatt cctggatatc aacgttgttg tcagcgtgct
     1681 caccaccgtt gatgatgttc atcatcggaa ccggcatgga gtatttgccc ggagtgccgt
     1741 tcagttcagc gatgtgctcg tacagcggca tacctttagc agctgcagca gctttggcgt
     1801 tagccagaga tacagccagg attgcgttcg cgccgaattt ggatttgttt tcggtgccgt
     1861 ccaggtcgat catgatcttg tcaatgccag cctgatcttt agcatctttg ccaatcagcg
     1921 cctgagcgat cgggccgttt accgcagcaa cagctttggt tacgccttta cccaggaaac
     1981 gggatttgtc gccatcgcgc agttccagag cttcacggga accagtagaa gcacctgacg
     2041 gagcagctgc cataccgacg aaaccaccct ccagatgtac ttcggcttca acagtcgggt
     2101 taccacggga gtcgatgatt tcacgaccga tgatttttac gattttggac attaggtttt
     2161 cctcaagtca ctagttaaac tgaaactcca gacaaacaac gcgtacccaa ggtacgcgtt
     2221 gccgttctaa cttttttact tacttcgcct gacgtttctg gaactcgctg gcggctttca
     2281 caaagcctgc aaacagcggg tgaccatcac gtggagtaga agtaaactcc ggatggaact
     2341 ggcaagccac gaaccacggg tgattcggaa cttcgatgat ctcgaccaac tgatcatccc
     2401 cggaacggcc cgcaacgcgc agacctgcat cttcaatctg tttcaacagc atgttgttga
     2461 cttcgtaacg gtgacgatga cgctcaacaa ttgtcggcgc attgtatagc tggcgaacca
     2521 ggctatcgtc aaccaactgg cactgctgtg cgccgagacg catggtaccg ccgagatcgc
     2581 tcttctcgct acgaacttca acgttgccgt tttcatcgcg ccactcggta atcagcgcca
     2641 caaccgggta cttacagtct ggcacaaatt ctgtagagtt ggcgttctcc atgttggcaa
     2701 catggcgagc gtaatcaatt aacgccacct gcatacccag gcaaatgccc agataaggaa
     2761 tattgttctc acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca cgatagccga
     2821 aaccgccagg tacgaggatt gcgtccagac ctttaaggat ttcaacgccg cgcgtttcaa
     2881 catcttgtga atcgatcagt ttgatgttga cgctgacacg attcttcagc ccaccgtgtt
     2941 tcagtgcttc gatcactgat ttataagcat ccggcagttc aatgtacttg ccgaccatac
     3001 cgatggtgac ttcacttacc ggattcgctt cttcgaagat aacctgttcc cattcggaca
     3061 gatttgcttc cgggcagttt aagctgaatc gtttacaaat ataatcgtcc agcccctgag
     3121 atttcaacag gcccggaatt ttatagatgg aatcgacgtc tttcagagaa ataaccgctt
     3181 tttccggaac attacagaac aatgcaatct tcgcacgttc gttcgccggt acagcgcgat
     3241 ctgaacgaca aatcaggatg tcaggctgga taccgatgga gagcagctct tttacagagt
     3301 gctgagtcgg tttggttttg acttcaccag acgctgccat gtacggcacc agcgtcaggt
     3361 gcataaacag agtgtgctca cggccaattt caacagccat ctggcgaata gcttcgagga
     3421 acggcaagga ttcgatatca cctactgtac cgccgatttc taccagtact acgtcatgac
     3481 cttcgccacc ttccagcacg cgctctttga ttgcgttagt gatgtgcgga ataacctgca
     3541 cggttgcgcc gaggtagtca ccgcggcgtt ctttacgcag aacgtcagag tagatacgac
     3601 ccgtggtgaa gttgttgcgg cggctcattt tggtacgaat gaaacgctcg tagtgcccca
     3661 ggtccaagtc ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt tggattgggc
     3721 tcatagtacc tggatcgacg ttgatgtaag gatccagttt catgatggtc acattgaggc
     3781 cacgggcttc aagaatggct gcgagggagg ctgcggcaat gcctttaccc agagaggata
     3841 cgaccccgcc ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga agttaggttg
     3901 aaaagacgat ggaataacca agacgggaaa gcagtatacc cgaacatgac ctgtgccaca
     3961 aactttcatt atccctcctc ttcgccagcg cactattgaa atcaggagtg agaaaatagc
     4021 ccctttgggg taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa
     4081 atggcgcttg accgcgtaat tcccttagag atcaatttcc tgccgtttta cctgttgcca
     4141 gacttcttcc attgtttcga ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat
     4201 acgctccact tcgcgaaaac gacgctcgaa tttttcgttc actttttgca atgcgatttc
     4261 tgctttcgtc cctaaatggc gagccagatt aaccgtggca aacagcaggt cccccatttc
     4321 ctcctccagt ttagcctggt cgacaacagc ctgccgcgcc tcgtacatca cctcgtcgat
     4381 ctcttcgtag actttatcaa ccaccgggcc aagcgtcgtc cagtcgaagc caacgttggc
     4441 gcaacgtttc tggatttttt gcgcccgcat taaagccggt aaactacgag gaatatcgtc
     4501 cagcgccgaa tgctgcgctt tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc
     4561 aagcacttca ctactgtttt cggcagaact atcagcaaaa acatgcggat ggcgacgctc
     4621 taatttatcg ctaatagcag cgcaaatatc attaaagtca aagcgccctt cttcctgagc
     4681 catttgcgcg taaaacacca cctggaatag cagatcgccc agttcaccgc gaagatcgtc
     4741 aaaatcttca cgggcgatgg cgtccagcac ttcgtaggtt tcttcaaggg tgtaaggcgc
     4801 aatggtggca aatgtctgct ctttatccca cgggcagccg ttttccggat cgcgcaggcg
     4861 ctgcataata gtgagcaaac ggtcgatttg attcattgaa ttgtcctgaa aattgcgggt
     4921 ctgtcaggtg taataccttc gagtaaaaga tatcgctgga tacggctgac gccatatcca
     4981 gctatgtaag actaactccc gtgcaaccga cgcgcgtcga taacatccgg cacctggttg
     5041 agtttaccca gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg
     5101 gtcgccagtt gctgtttggt gtcgctacgg ctggcaacgc caagcacgtt caccttctcg
     5161 ttggcgagaa tggtcgtgat atcacgtaac aacccactac gatcattagc caccacgcgg
     5221 accaccagcg aatatccggc ggagtagctc tcaccccata ccgcgtcaac aatgcgttct
     5281 ggcgcatggg agcgcagttc cgccagttgt tcgcaatcgg cgcggtgtac tgaaataccg
     5341 cgtccctggg taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg
     5401 atgtggtgca tcaggttacc aacaccttca accaccacac gaccgttatc tttactgcgg
     5461 ttttgcggtg tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg
     5521 gcactcggct tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc
     5581 ccgccaccaa tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga
     5641 tgtttttctg cttctttcag gctgatcccc agatgttcca gctcgtcgtc aaggatttgc
     5701 cgcccagcca gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa
     5761 cgcccacggc tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc
     5821 ggctgtttct gggtgataat ttcaatctgg tcgcccattt gcagctggta ggtgaacggc
     5881 acaatgcgcc cgccaatttt tgccccgatg cagcggtgtc cgacatcact gtggatgtgg
     5941 taagcgaagt ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttcggcgta
     6001 aagacataca cccggtcgtc aaacacctga ctacgtactt cgtcgagcat ttcgccggaa
     6061 tcagccatct cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt
     6121 cccgaacgtg cgccgccagc tgccgcgccc tctttatatt tccagtgcgc agcaacaccc
     6181 aactccgcat cttcatgcat ctgtttggtg cggatttgga tctcaacggt ttttccaccc
     6241 ggccccagaa ccacggtatg aatagactga taaccgtttg gtttcgggtt agcgacgtaa
     6301 tcgtcaaact catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa
     6361 caatcctgta aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag
     6421 gcgaggttct ttttctgcat tttgcgccag atgctgtaga tgtgtttcgg acgaccatac
     6481 acttcagctt taacgccttc agctttcatc tcagcgcgca gatggccgac aaactcttcg
     6541 atatagtgtt cgcggtcgag acggcgttca tgcagcagtt ttgcgatgcg tttgtattcg
     6601 gtcgggtgga gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca
     6661 agacggttcg ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacacgttca
     6721 tcttccggcg catcttttac ttcgcgcaga tgagcaatac gctccgccag tttgatgact
     6781 acgcagcgaa aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg
     6841 gaggaaacag aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg
     6901 ccgtgaataa ggttaacaac cgacttaccg acgctctcac gcagcacatc ttcgctgact
     6961 acgttggcat cagccagagg gaacagcagc gccgcccgca gcgtgtcaat gtccatactt
     7021 aatgtcgaga ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga
     7081 tgcccctgcg tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc
     7141 tggctggtaa tacccagact tgcgatccat ttttccggat caaattcacc agccttattg
     7201 atatgtgcac ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat
     7261 cggcaaatcg taactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt
     7321 gtgggaacat atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt
     7381 cgctatcccg agccagcgtt gcagggttac aggatacata aactatacga ataggttcca
     7441 gttttataat ttgctgcata acacctgcgg cacctgctcg cgccgggtcc agcaacactt
     7501 tatcgaagcc gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat
     7561 aaaacgtcac attctgtaag ccattaagac gcgcattctg ctggcctttt tccaccagcg
     7621 caggaacacc ttctacaccg accacactgg cagcttgtgt cgccaatggc agtgtaaagt
     7681 tgcccatacc gcagaacaga tccagtacgc gatcttcagg ttgcacatcc agccattcca
     7741 acgcacgcgc taccattttt tggttcacac ccgcattgac ctgaataaaa tcgcgcgggc
     7801 taaaagttaa gcgcaacccg tttgagtcat accagggcat ctcaccagag acggtttcga
     7861 gtatctcact atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcgttcca
     7921 gtttttcgcg atctgccgaa cttagcggtg cggtatggcg caaaatcatc agcgtgccgc
     7981 tggttgcctg taccagttca acatgaccaa gatggcgcat agcttgtaag ctgcccagac
     8041 atgccctgac tttgggcagc aatgcttcaa gttggggcgc taaaatgggg cattgtttga
     8101 cgtcgacaat gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg
     8161 gtaagtagtt cagacttaaa cgcgcgcggc ggcgatagcc ccagggaaca tcggcgatca
     8221 cttcagagac atcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta
     8281 aatccacgct ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg
     8341 gcgtttcgcg ttccgggcta tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt
     8401 ttttatcttc agtaacagca acttccgcgt tttcctgcgg caataatccg gggataaata
     8461 acgttttgcc gttatgtcgc gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa
     8521 cggttatgat ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg
     8581 agactttctc aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac
     8641 tacagcctgc gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta
     8701 ttgctgagta tctttttcgt cgtgcatcgc tataacgact tgcagcgtca actggaagat
     8761 gccggtgcta gcattattga gccgcttgca gtttctactg aatatggcat gagcctgcaa
     8821 aatcgcgaat ctatcggtca gttaataagc gtactgcatc gtcgccattc cgatattgtt
     8881 cgcgcgattt cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt
     8941 gatccctcat caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact
     9001 cgtgacggcg atattatgat cctccgcacg ccgattattt ctgagagtta ctcccccgac
     9061 gaatcgccca gtagcgatgc caaaaatagt caaaatatgt tgggatatat tgcgcttgag
     9121 ctggatctta aatcggttcg cttgcagcaa tataaagaga tctttatttc ctgcgtgatg
     9181 atgctgtttt gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc
     9241 ggtccgattc gcaacatggt gaataccgtc gaccgcatcc gtcgcgggca actcgacagc
     9301 cgggtggaag gatttatgct cggcgagctg gatatgctga aaaacggtat caactcgatg
     9361 gcaatgtcgc tggctgctta tcacgaagag atgcagcaca atatcgacca ggcgacgtcc
     9421 gatctgcgtg aaacgctgga gcagatggaa attcagaacg ttgagttgga tctggcgaaa
     9481 aagcgcgccc aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag
     9541 ctgcgtacac cactgaatgg tgttattggc tttacccgcc tgacgctgaa aacagaatta
     9601 acaccaacgc agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca
     9661 attattaatg atgttctcga cttctcgaaa ctggaagcag gtaagctgat tctggaaagt
     9721 attccattcc cactacgcag cacgctggat gaagtcgtta ctctgctggc acattcttct
     9781 cacgataaag ggttggaact gacgctcaat attaaaagcg acgtgcctga taacgtgatc
     9841 ggcgacccac tgcgattaca gcaaatcatc actaacctgg tggggaatgc aattaaattc
     9901 accgagaatg gcaacattga tattctggta gaaaaacgtg cgctgagtaa taccaaagtg
     9961 cagattgaag tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc
    10021 ttattccagg ccttccgaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg
    10081 ctggggctgg tgattacaca aaaactggtt aatgaaatgg gcggcgatat ttcgttccat
    10141 agccagccga atcgcggttc aactttctgg ttccacatta atctcgatct gaacccgaac
    10201 attattatcg aagggccatc catccagtgc ctcgcaggta agcgcctggc ctatgtcgaa
    10261 ccaaactccg cagcagcgca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg
    10321 gtttatagcc caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttgttaggc
    10381 atcgcggtga ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcggta
    10441 tcgatgaccg atttcctgat gctggcactt ccttgccatg cacaagttaa tgctgaaaaa
    10501 ctcaagcaag atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg
    10561 cctgccctga cggaattttg tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa
    10621 agtaagctgg caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc
    10681 ggcgcattgc tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggca
    10741 gttgaacggg cgaaacagat gccgttcgat ttgatcttaa tggatattca aatgccggac
    10801 atggatggca ttcgggcctg cgaactcatc caccaactcc cgcatcagcg acaaacgccg
    10861 gttatcgcgg taacggcgca tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg
    10921 atgagcgatt atctggcgaa accgattgaa gaagagcgat tgcataattt gttgttgcgc
    10981 tacaagcctg gcagcggtat ttcgtctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg
    11041 gtgaacccga atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc
    11101 gatttagcgc gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa
    11161 gttgaggaac agctggctgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg
    11221 catggcagtt gcggctatag cggcgtacca cgtatgaaga atctctgcca gctgatcgaa
    11281 caacagctac gtagcggtac taaagaagaa gatttggagc cggagctgct ggaactgttg
    11341 gacgagatgg ataatgtcgc gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt
    11401 cgagtagcaa tcggcttttt atgacgctgg tttcagattg gtgacaaagt gcctgtgttt
    11461 atgccggatg cggcgttaac gccttatccg gcctacaaaa ccaattaaat tcaatgaatt
    11521 aaaaaatgat gtagacctga tacatagcac aacaggctat tttgcgttta gcatcagtct
    11581 caaaccggct ccagatagag ccggttttgg ttttctgtct taacgcacca tgcacgggcg
    11641 cttgttatcg aacgtccagc caggaatcag atactgcatt cccatcgcat cgtcacgcgc
    11701 gccaagcccg tgtttctgat acagctcatg ggctttcatc acttgatcca tatcgatttc
    11761 tacacccagc cccggttttt ctggcacctg taccagcccg cctttgatct caaacggttc
    11821 tttggtcagg cgctgattac cttcctgcca gatccagtgc gtatcaatag cagtaatttt
    11881 acccggtgca gcggcggcaa catgggtaaa catcgccagg gaaatatcga agtggttgtt
    11941 tgagtgtgaa ccccaggtca ggccaaattc atggcacatt tgcgccacac gtaccgaacc
    12001 ttgcatagtc cagaaatgcg gatccgccag cgggatatca acggattgta acgagagcgt
    12061 atggcccatc tgccgccagt cggtggcgat catattggtt gcagtcggca ggccagtcgc
    12121 gcgacggaac tctgccatca cttcacgccc ggagaaacct tgctccgcac cacacggatc
    12181 ttctgcataa gccagcgaac ctttcaggta tttaccaatt ttaattgctt cgttcagcga
    12241 ccaggcaccg ttaggatcga gcgtaatacg cgcctgcggg aagcgttgcg ccagtgccac
    12301 aatagactcg gcttcttctt ccccggccag tacaccgcct ttcagtttga aatcgttgaa
    12361 gccatatttt tcatacgccg cttccgccag gcgcaccacc gcatccggcg tcatcgcttc
    12421 ttcatgacgc aggcgatacc agtcgcatga gtcatccggc tggctttgat acggcagagg
    12481 tgtggctttg cgattaccga cgaagaacag ataaccgagc atttcgactt cgctacgctg
    12541 ttgaccatcg cccagcagcg atgccacgtt tacccccaga tgctgcccca gcagatccag
    12601 cattgccgct tctatcccgg taactacatg aatagtggta cgcaaatcaa atgtctgcaa
    12661 accgcgccca ctagcatcac gatcggcaaa agtattacgc accagcgtca gaacgttttt
    12721 gtattcaccc agcgttttac ctaccaccag cggaatcgca tcttccagcg ttttacggat
    12781 tttctcgccg ccgggaattt cccctacgcc agtgtgaccg gaattatctt tgataatcac
    12841 aatattacgc gtgaagaacg gtgcgtgtgc accactcaga ttcatcagca tactgtcatg
    12901 acccgccacc gggatgacct gcatttcagt aacaacaggc gtcgtaaatt gagaactcat
    12961 atttatgtcc tttttcagaa tcaatgacgg ccgaaaacgg gacgtttacg gtcaaaggtc
    13021 cagccgggga tcaggtactg catcggacct gcgtcgttac gcgcaccgca aggcagacgt
    13081 ttataggcct catgtgcctt ttgtacctgt tcccagtcca gttccacgcc cagaccgggc
    13141 gcatcaggaa cggcaatttt tccgttttta atctccagcg gatttttggt caggcgacaa
    13201 tcgccctcct gccaaatcca gtgggtatcg atagcggtag gattacccgg tgccgccgcg
    13261 cccacatggg taaatatcgc cagagagata tcgaaatggt tgttggaatg gcagccccag
    13321 gtcagccccc agtcgtcgca aagctgcgcc acgcggactg caccggaaag cgtccagaag
    13381 tgcggatcgg caagtggaat atctaccgca ttgagcatca ccgcatgacc catttcgcgc
    13441 cagttggtgg cgatcatgtt agtcgcgacg ggcaagccgg tcgcccgtcg aaactccgcc
    13501 atcacttcac gtccggagaa gccctgttct gcgccgcatg gatcttcggc ataggtaaga
    13561 acatcattca gccctttgca taaagaaatg gcttcatcaa gcagccatgc accgttggga
    13621 tcaacggtaa tccgcgcatc cgggaagctt ttcttcaatg cacgaacagt gtcgatttct
    13681 tgctcgccag gtaacacccc gcccttaagt ttgaaatctt taaagccata gcgatcctgt
    13741 gaggcttccg ccagacgcac aacggcttcg ctgttcatcg ctttctgatg gcgcaactga
    13801 taccactcat ggttgcccgg cgtattttcc agataaggaa gatcggtttt ggtccgatca
    13861 cctatataaa acagataacc gaggacggta atagtctcgc gttgcttgcc tggccccaac
    13921 agttcgcaga ccggaacatt cagcgcctta cctagcaggt caagcaaggc ggcttccagc
    13981 gccgccacgg cattaacgcg caattcaaaa gtccaggcac ctttgccaaa ggtatcaaaa
    14041 tcggctgcct gattaccttt atgtacctgc tgaaccactt tattcaggcg cgcaacttcc
    14101 tggcccagaa ccatcggaat agcatcgacc agtgtttgat aaatcacctc tccgcccggc
    14161 gcttcaccaa tgccggtatg cccggcgtta tcggtgagta ccacgatatt gcgggtgaag
    14221 tatgcgttat gcgcaccacc aatattgagc aacatgctgt catgtccggc gaccggaatg
    14281 actttcatat cagtaataac aggactggat tgtgtcgcca tcttattgcc ccgcgacagg
    14341 tttcagctcg atacgcttga tatcgcccac cagcaccagg tagctcagta ccgcgattag
    14401 ggcatgaaca ccaacataaa tcagcgcccc attaaacgag ccagtcgtgc caacgatata
    14461 accaattgcg attggcgtga cgatgccaga aatgttaccg aacatgttga acaggccacc
    14521 ggaaagaccg ctgatctctt ttggcgcggt atctgccatt actgcccaac ccagcgcccc
    14581 gatgccctta ccgaagaagg ccagcgccat aaagccgatg atcatccact caacgttgac
    14641 gtagttgcag aacaccatca ccatcgacag caacatgccc attacgatcg gtgttttacg
    14701 cgcaatgttc agcgatcccg tgcggcgcat cagccagtcg gaaataattc cacccagcac
    14761 accgccgata aaaccgcaaa ccgccggaac ggaagccaca aagcccgctt tcagaatcga
    14821 catcccacgc gcctgcacca gataaaccgg aaaccaggta atgaaaaagt aagtcagggc
    14881 gttgatacag tactgaccga tataaacgcc gatcatcatc cgtgacccga gcagctgttt
    14941 gatctgcccc cacttcacgc tgaacggcac tttaactttg gtgctttgct gatccatatt
    15001 gatcagcgcg ccacccgcgg cgatatactc cagctctttc tgatttaccc ccggatgttg
    15061 atttggctcg tggatgactt tcaaccagat aaagctgatg acaatgccca gaccgcccat
    15121 aaagaagaag acgtgtgacc agcccacttc atgcgtcagc cagcccataa tcggcgcgaa
    15181 gatcaccgtt gcgaagtatt gagcggagtt aaaaatcgac accgccgttc ccctttcctg
    15241 caccggaaac caggccgcaa caatgcgact gttgccgggg aaagatggcg cttcagcaag
    15301 cccgaccagg aagcgcagag taaacagggc aacgataatg ccgaatccac taaagatatc
    15361 gacgaagcct tgcagcaagg taaacatcga ccagataaag atcgaccaga agtagacgcg
    15421 ttttgaacca aaacgatcca gcaaccaacc accagggatc tgcccgataa cataagccca
    15481 tgagaaggca gagaacacat agcccattcc cacgggatca aggccgatat ctttggccat
    15541 ttccgaaccg gcaatagaaa gcgtagcgcg gtcgccgtag ttgaaggatg tgacgataaa
    15601 caacatcacc actatccagt aacgagcatt cgtgcgtttt tccacactgc tcgcagcctg
    15661 acttaaagaa ctcattgttg cactcctgaa aattcgcgtt agccacgctc actctggact
    15721 gcgacatcgc caggaaatca gaggtgacgt agggtgtttt ttgccgtttt tataggtcgt
    15781 tcgccgaata cggcgcgtgt ttatatctgg caatagcagt ataaaaagcg cgccatagcg
    15841 gctcaccgtg caacaacaca acattaatgc gttcaatgag gcccgatttt ggcattagcc
    15901 ctggacggtg gaatccactt cacggaaatg aaaacaagaa caagaaagga agggtaaaaa
    15961 cgaagaaata aagaagagta tgaaatggat cgcttgactc taggcaaacg ccagtaaaaa
    16021 tccgcgctat gaagcagttt ttactggcat ttgcctgaaa agattcgatt cagcaccgct
    16081 aaaacgacat ttaccgctcg ctgacatatc aggacaacag cgtgccccac tgttcgaccc
    16141 acggatttga ttccgtttcc ggttccgggt tctcactggc gtcaatcaac agcatttcgc
    16201 caacccgctg agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg ccattgcaaa
    16261 aatttacata actgctgtcg ccgagcgcaa tcacgccata acgcagattc ggctggaagc
    16321 ccagactatc tttgattccc tgaaagagcg gcacaatgct atcaggaagg tcgccctgcc
    16381 cggtcgtgga cgtaaccacc agaacatact tatcctgata gggcagccag tcgcttaatt
    16441 caggatcttc aaataccgtt gctttgtggc cctgtgcggt cagaattgct tctgcttctt
    16501 ccgccactaa cagcgaattt ccgtacatgg tgccgacaaa aataccaatt tccgccatac
    16561 cgttttctcc ctggattagg aacttatctc tccatcctga cccgatggcg cactgaactc
    16621 aaccctttca ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg cctgcatcca
    16681 ggtgtcgtcc aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat gcgtcaatga
    16741 caactgactg gcatgtagca ttaaccgctg gaggccaaaa tgctcagcac cgctgcgatt
    16801 ctggcgtaaa tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat gggcaagatg
    16861 tcgtcgaagc tgatgtttgc gtccggtttt cggctccagt tccaccaggc cgtagcgcgt
    16921 ggtcgggtaa cgtccggtcg ctaccggcat ttctacggtc gccagaccgc gataatgcgt
    16981 cactgctggc tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt tgtccagttc
    17041 ttccaccagt ggataatcca acaccgcttc ttccatcaac cagccgcgca caatcgcatg
    17101 gtaacgtttc tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc cggcctcgct
    17161 ggataatccc atcaacaaca caccagaagt gggtcggtcc agacgatgag cagtaaaaac
    17221 atgctgtcct atctggtcac gcacggtttg catgaccact actttctcgt cgcgatccag
    17281 ccagctacgg tgaaccagcc agccggaggg tttatttacc gcaaccagcc attcatcctg
    17341 atagagtatt tccagcatta gctcgcatca tccgcaaaaa gagcatccag tttttccaac
    17401 tcagccagaa taagcgcgcg ttgcggatgg tccgtcgcca gcgccatttc ataatagggt
    17461 gcaacagcaa aagcgcccgg taacggctgg ttgttattta acaaatcgtg cattcgcggg
    17521 atcagccccc actgcaacca ctccagtggt tccatggtgt ccataaagaa cggttgggta
    17581 ctattaaatt gatgcggctg gggttcatcg tttcgccagt gctgatgttc acgcagtaac
    17641 gcttcaagcg cctgcaactg gagacgaacg cggtcatgag tggtcataaa gcaacctcaa
    17701 taagaaaaac tgccgcgaag gatagcagga ataaaaaaag ggagcactgt attcacagtg
    17761 ctcccggttc gtttcgcagc attccagcta cttttgttgc tccctgctca tccttgacaa
    17821 cttttcctct ggccttgcgg ccaatcgttc atcctgaact attgcttcct gctcacacca
    17881 ccccgatgtg atacttcatc ctgaagtgtc cctggccttc ctgacccacc gaatcatcct
    17941 gaccggttct cattctccat cctggaggtg tcctttaacg cgtcctgcgt catcctcttc
    18001 gcttcatcca gaagcctttc cctgaaacac catcctggtg tgtcctgcag aagtgtcatc
    18061 atcctgatgt tcacttcgtt gtctgactcc ctgtcgacga agatagaatc gtctttttcg
    18121 cgaagtctta caaggcgctt acagacaatg ccttaaggaa aatttcatat gaaagttaag
    18181 aattaacaat caacattttg attttaaatg aaaataacga ttcttatctt tttaagtatt
    18241 cgcttatttc ctggcgatct cctacagagt ctgtaagaga tctctcacaa ccagatgggg
    18301 tatttggatt acagaagagg cttaagttga ttaaggaatt ccgcaagatt tgaggctaaa
    18361 ttcgtccgtt tacgtgttcc cagcgtctct ttgcacactt caccggagag attacacacc
    18421 gagatgacct ctaactcttc ttccagcgtc gcgataaata gcgtgggcgg cagttttagc
    18481 cttttctgag tcaccagatg accaatcaga ttttcctgca cccgacggaa gtcgtcttca
    18541 ctccaggttt gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat atccccggca
    18601 aactgagtcg tataaaaggt atgaattgtg ggttgtatca caatatcaaa agcgcgttca
    18661 accgcgttta cattttgttc cccggtaaac ggctggggtt gccagtatac ggcatcttcg
    18721 gtagttgaaa taatgcacgg tgaaggaaca ccgtacaatt cctcacttaa cggccagctt
    18781 ttatgctctt catgccacgc atcgcagtag cgtgcagtaa aatctttcag ggcttgtgcg
    18841 gtcaaatcgt ccactaattt ctctcttcac ttaaaccaga tacacttgtc gtttagttta
    18901 tctggtttat gacggtgaaa catgtcttct tatgcaaacc atcaggcact tgcgggcctg
    18961 actcttggaa aatcaaccga ttaccgggat acctatgacg ccagcctact gcaaggcgtt
    19021 ccacgcagcc tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc ttttcacggt
    19081 acggatatct ggacgctgta tgaactttcc tggctgaatg cgaaaggttt gccgcaggtc
    19141 gctgtcggtc atgttgaact tgattacacc agcgtaaatc tgattgagtc gaagagtttt
    19201 aagctctatc tcaacagttt taaccagacg cgttttaata actgggatga ggtgcgccag
    19261 acgctggagc gcgacttaag cacttgcgct cagggtaaga ttagcgtggc gttatatcgt
    19321 cttgatgaac tggaaggcca gccgataggt catttcaatg gcacttgcat tgatgaccag
    19381 gatatcacta tcgataacta tgaattcact actgactatc tggagaatgc caccagcggt
    19441 gaaaaagtag tggaagagac gctcgtcagc cacctgctga aatcaaactg cctgatcacc
    19501 catcaaccag attggggttc gatccaaatt cagtatcgtg gacgccaaat tgacagagaa
    19561 aaactgctgc gttacctggt ctcattccgt catcacaacg agttccacga acagtgcgtg
    19621 gaacgcatct ttaatgacct gttacgcttc tgccagccag agaaattgag cgtttacgca
    19681 cgttataccc gtcgtggcgg tctggacatt aacccgtggc gcagtaatag cgattttgtc
    19741 ccatcgacca caagactggt tcggcaataa attttttctc aattttgcgt gctggattca
    19801 cgcagaaggt tgtgaaaggt catcaggcag ggctattgta atcaaaggga atgacgatat
    19861 tcgtcccata aggagttttt cttgattaca catattagcc cgcttggctc catggatatg
    19921 ttgtcgcagc tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct ctatcaactg
    19981 tttcgcaact gttcacttgc cgtactgaac tccggtagtt tgaccgataa cagcaaagaa
    20041 ttgctgtctc gttttgaaaa tttcgatatt aacgtcctgc gccgtgaacg cggcgtaaag
    20101 ctggaactga ttaatccccc ggaagaggct tttgtcgatg ggcgaattat tcgcgctttg
    20161 caggccaact tgttcgcggt tctgcgagac attctcttcg tttacgggca aatccatcat
    20221 accgttcgtt ttcccaacct gaatctcgac aactccgtcc acatcactaa cctggtcttt
    20281 tccatcttgc gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat ggtggtctgc
    20341 tggggcggtc actcaattaa cgaaaacgag tatttgtatg cccgtcgcgt cgggaaccag
    20401 ctgggcctgc gtgagctgaa tatctgcacc ggctgtggtc caggagcgat ggaagcgccg
    20461 atgaaaggtg ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg ttttattggt
    20521 atgacagagc cgtcgattat cgccgctgaa ccgcctaacc cactggtcaa cgaattgatc
    20581 atcatgccgg atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca cggcatcatt
    20641 atcttccctg gcggtgtggg tacggcagaa gagttgctgt atttgctggg aattttaatg
    20701 aacccggcca acaaagatca ggttttacca ttgatcctca ccggcccgaa agagagcgcc
    20761 gactacttcc gcgtactgga cgagtttgtc gtacatacgc tgggcgaaaa cgcgcgccgc
    20821 cattaccgca tcatcattga tgacgccgct gaagtcgctc gtcagatgaa aaaatcgatg
    20881 ccgctggtga aagaaaatcg ccgtgataca ggcgatgcct acagctttaa ctggtcaatg
    20941 cgcattgcgc cagatttgca aatgccgttt gagccgtctc acgagaatat ggctaatctg
    21001 aagctttacc cggatcaacc tgttgaagtg ctggctgccg acctgcgccg tgcgttctcc
    21061 ggtattgtgg cgggtaacgt aaaagaagtc ggtattcgcg ccattgaaga gtttggtcct
    21121 tacaaaatca acggcgataa agagattatg cgtcgtatgg acgacctgct acagggtttt
    21181 gttgcccagc atcgtatgaa gttgccaggc tcagcctaca tcccttgcta cgaaatctgc
    21241 acgtaatctc ccctcc
//
LOCUS       DAZXUL010000067        20892 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZXUL010000067 DAZXUL010000000
VERSION     DAZXUL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20892
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.067"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="ACH3L1_002531"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="ACH3L1_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFT4077228.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="ACH3L1_002532"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="ACH3L1_002532"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFT4077229.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="ACH3L1_002533"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="ACH3L1_002533"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFT4077230.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="ACH3L1_002534"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="ACH3L1_002534"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFT4077231.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACH3L1_002535"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACH3L1_002535"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFT4077232.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACH3L1_002536"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACH3L1_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFT4077233.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="ACH3L1_002537"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="ACH3L1_002537"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFT4077234.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="ACH3L1_002538"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="ACH3L1_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFT4077235.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="ACH3L1_002539"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="ACH3L1_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFT4077236.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="ACH3L1_002540"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="ACH3L1_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFT4077237.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9181..11403)
                     /gene="rsxC"
                     /locus_tag="ACH3L1_002541"
     CDS             complement(9181..11403)
                     /gene="rsxC"
                     /locus_tag="ACH3L1_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFT4077238.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(11396..11974)
                     /gene="rsxB"
                     /locus_tag="ACH3L1_002542"
     CDS             complement(11396..11974)
                     /gene="rsxB"
                     /locus_tag="ACH3L1_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFT4077239.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11974..12555)
                     /gene="rsxA"
                     /locus_tag="ACH3L1_002543"
     CDS             complement(11974..12555)
                     /gene="rsxA"
                     /locus_tag="ACH3L1_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFT4077240.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12632..13072)
                     /locus_tag="ACH3L1_002544"
     CDS             complement(12632..13072)
                     /locus_tag="ACH3L1_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFT4077241.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13158..13373)
                     /gene="ydgT"
                     /locus_tag="ACH3L1_002545"
     CDS             complement(13158..13373)
                     /gene="ydgT"
                     /locus_tag="ACH3L1_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFT4077242.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13646..13771)
                     /gene="blr"
                     /locus_tag="ACH3L1_002546"
     CDS             complement(13646..13771)
                     /gene="blr"
                     /locus_tag="ACH3L1_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFT4077243.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            14014..15054
                     /locus_tag="ACH3L1_002547"
     CDS             14014..15054
                     /locus_tag="ACH3L1_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFT4077244.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(15090..16091)
                     /gene="add"
                     /locus_tag="ACH3L1_002548"
     CDS             complement(15090..16091)
                     /gene="add"
                     /locus_tag="ACH3L1_002548"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFT4077245.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16195..17367)
                     /gene="malY"
                     /locus_tag="ACH3L1_002549"
     CDS             complement(16195..17367)
                     /gene="malY"
                     /locus_tag="ACH3L1_002549"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFT4077246.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17377..18969)
                     /gene="malX"
                     /locus_tag="ACH3L1_002550"
     CDS             complement(17377..18969)
                     /gene="malX"
                     /locus_tag="ACH3L1_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFT4077247.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19144..20172
                     /gene="malI"
                     /locus_tag="ACH3L1_002551"
     CDS             19144..20172
                     /gene="malI"
                     /locus_tag="ACH3L1_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFT4077248.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            20284..>20892
                     /gene="hdhA"
                     /locus_tag="ACH3L1_002552"
     CDS             20284..>20892
                     /gene="hdhA"
                     /locus_tag="ACH3L1_002552"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFT4077249.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
BASE COUNT         5318 a         5263 c         5365 g         4946 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat tagcctgttg
     9301 ctgttccagc ttgcgtgctt tggcgcgggc aatagcggct tcgacggcag ctttgcgcgg
     9361 atcaacctgt tcttctggct ccgcattggc ctgttgctgt tccagcttgc gtgctttggc
     9421 gcgggcaata gcggcttcga cggcagcttt gcgcggatca acctgttctt ctggctccgc
     9481 attggcctgt tgctgttcca gcttgcgtgc tttggcgcgg gcaatagcgg cttcgacggc
     9541 ggctttgcgc ggatcgacct gttcttctgg ttccgcatta gcctgttgct gttccagctt
     9601 gcgcgctttg gcacgggcaa tagcggcttc gacggcggct ttgcgcggat cgacctgttc
     9661 ttctggttcc gcattagcct gttgctgttc cagcttgcgc gctttggagc gggcgatagc
     9721 tgcttcaacg gcagttttac gtggttcagc aacggttgct gcgtcgttag tttgctccag
     9781 ttctgcctgt ttcgctctgg cttgcgcttt acgggcttcc cgttctgcaa taattgcact
     9841 gttatccggg cgttcgcccg ctttaatcac aataggctgt gtagcctggg cctgtttctc
     9901 tttcacccgc gccagagcgg cagcaatcgc atctttatct ttggctgcag gttgaacggc
     9961 tgcgctctta tgtcgttcaa ggcgagtcgc tttttcgcgc tccagacgag cctggcgcgc
    10021 ttcgaaacgc gctttggctt ctgcggcgcg cttttcttcc tgacgaatag ccgcaatttc
    10081 agctttttcc tgacggaaat attgcaccaa gggaatattg ctcgggcaaa cccacgcgca
    10141 agccccacat tcaatgcagt cggcaatgtt atgcgtggta gctttatcgt gttgctgacc
    10201 tttgctgaac cagtacaact gttgcggcaa aagatccaca gggcaggcgt cagcacaggc
    10261 gctacaccgg atgcagcttt gttcttcctg tggttcgcca agttcattgg cagagggagc
    10321 caacagacag ttggtaatct ttacgaccgg gacatccagc catggcaagg taaagcccat
    10381 tagcgggcca cccataatca ccatttgatc ggcagagggg cagaatccgg catcattcaa
    10441 taaatgacgc actggcgtcc ccagccgtgc ccagacgttg cccgggcgag cgattgcttc
    10501 gccagtcagg gttacaacac gctcggtaat cggctcgcca tcaataacgg cacgtttcac
    10561 tgcataagca gtgccgacgt tttgcattaa tacgccgata tcggatgaac gcccgccatg
    10621 tggaacctgc ttcccggtca gaatgtaggt taattgttta gcaccgccag aaggatattt
    10681 ggttggaatc acccgcagag aaatatcgtt agagtccgcc agcaccgcgc gcagcatgga
    10741 aatcgcctgc ggtttgttat cttcaatgcc gataagaatt tcgcgtggct gcagaatatg
    10801 cgcaagaatg cgaataccct ctacgacctg agccgcgcaa tcctgcatca aacggtcatc
    10861 ggcggtaatg tacggctcgc actcagcagc gttgataatc aacgtttcaa tcttatctcc
    10921 accaccctgc aatttaacgc ctgtcgggaa tccggcaccg cccagcccgg caacaccaaa
    10981 ctgatgtatg cgctcgatta actcttcgcg actgcgagag cgataatcga cccagccgtc
    11041 gcgcgggatc cagcagtctt caccatcggc atcaataatc acgcttaatt cagctaaagc
    11101 tgaaggatga gccgtagagt ggggcgcaat agccgtaacg gtacccgagg tgggcgcgtg
    11161 aacaggcagc attttgccgc gaccacgggt aagcggctgg ccgcgcaata ctttatcgcc
    11221 gacgctaacg cacaactcac cttcagcgcc aatatgctgt ttcagtggaa taacaaaatg
    11281 ctgcgccagt agtacctggc gcaggggggt accgttggtc tgggttttca tctccggtgg
    11341 atggatgccg ccgttgaaat cccagatttt attttttctg aatgcagaga ataacttaag
    11401 catggtgttc cacgggaatg atacgcacgg gaatggtgtt cagatcccat ttccaggagt
    11461 caggtgtttc tgcgaccggt tgcaacgaga tgcagtgcgt cgggcacgga tcaacacata
    11521 aattgcagcc cgtacagaga tcactcatta ccgtatgcat gacacgggta gcgccaacga
    11581 tggcgtctac cggacaagcc tgaatacatt tagtgcagcc aatacagtta ttttcatcaa
    11641 taaccgccac catccgcgca ggcgttagct cttgcgcttc gccatccagc ggctgcggct
    11701 cgacattaag caactcggca atttttagca tcacagcttc gccacctggg gcgcaacggt
    11761 tgattttttc accgttacag ctgatggctt ccgcgtaggg gcgacagccg ggataaccgc
    11821 actgaccaca ctggctctgc ggtaagattt cgtcaatttt ctcaacgacc ggatcgtctt
    11881 ccaccgcaaa acggcgggag gcataaccca gaatggcgcc aaacgccagg cccagcaggc
    11941 tcacggcggc aacggcaatc cagatagcat tcattacaac ttcaccaaac cactaaagcc
    12001 cataaaggcc agagacataa gacctgcggt aattaacgca atggcattac cgcgaaaagg
    12061 tgccgggaca tcagccacag caaggcgttc gcggatggcg gcgaagagca ccatcaccag
    12121 cgagaaaccg acagcggcgg aaaaaccgta cagcgccgac tgcaagaaat tgtgcccgag
    12181 attgatattc agcaacgcca cgccgagcac tgcacagttg gtggtgataa gcggcaaaaa
    12241 aatccccagc agacggtaaa gcaccgggct ggttttgcgc accaccatct cggtgaactg
    12301 cacgaccaca gcaatcacca gaataaatgc cagggtgcgc aggtaaatca gattaagtgg
    12361 gatcaaaatc cacgtatcga taagccaggc gcaaatagac gtcagcgtca tcacaaacgt
    12421 tgttgccagc cccatgccca tcgcggtttc cagttttttg gaaaccccca taaacggaca
    12481 gagaccgaga aacttgacca gtacaaagtt attgaccagt acagttccga caaacagtaa
    12541 caggtagtca gtcatttttc ggcctgaaat aaaaaagccg cctattatcc gttaatccag
    12601 agcaggcgac aacaggttaa ctgtaaggtt attacggatt cacaaacgtc gcttttaccc
    12661 gcgacgagcg tttgaaatag gggacgatca atgcggttgc caacaaagta aacagcaact
    12721 ggcgaacggc tatgccaact tccacgggtg aaaaagcgaa tgcttttact gccagcaata
    12781 cggaaataag cagccagata atatagtgtt taggaacaca acgccgacgt ttaaagaaag
    12841 cgatggtcaa ccacagtgtg taataccaca tggcgatagc ggtaataaaa gaaacgcccc
    12901 ataagatttg tgtcgtcagt gcttgtccgc cgagcgtttg aaatgtttgc ggagaagata
    12961 acgcagcggt atacagcaac aacgccagcg tcgtactcaa cagagcgact aaaagccagg
    13021 ccaacgggcc aagcaaccag cctccaatac gttgtggcgt tgtggtggtc ataggttctc
    13081 cagaatagcg acgcaaacag gcaaatctaa ttttaggggc gttattataa ggcttttata
    13141 accgatcgct actttcttta ttggacatag tgccagacgg acttcggtac ctggccgagg
    13201 tcaaacaaac gcccgccaga aaccagctct gcgcgacggt gatcggcagc acgatacata
    13261 ttgatcagtt cctgatcgtc cgtcagggtg taattaagat ggtcgtagag tttttccaga
    13321 ctttcgagtg aactgatttt gcgaaatttt aataagtagt cctgaacagt cataaaaggt
    13381 ccataaaata attaatacca aaggggtagg tattattagt gatgataaaa tttcaccaaa
    13441 agtgaacgct tgtgttatta caataataag aatgcgctga tggcaaatat tcagggatga
    13501 aaaactgctc tgaaaagagt aacgcgcttt actatccctg acaatcacca acaacatcga
    13561 acaagataat aaattcctgg tttaatatcc gacaagtgaa aacatgcacc cggacgggca
    13621 gcatgtcgct ccacaagtgc agagcttact tgtgttgtac cgaagcactc tgttcaggtg
    13681 gctgatagtt gtcaatgtga ctcgccacgc caagaagaat gactgaaacg acaagaacga
    13741 tccaacctgt taattcaata agacgattca ttacagccca catctctctt gattgatcca
    13801 ttaacttcag ggggtaaatg ttacttagca ataatagctc cagcaagatt tttactgagg
    13861 tttttgcgat atttagcttt tgtcgttgga aaattcgcta tttttttgac ttaagttaaa
    13921 caacatccct tattgctggc aggttattaa actgttgagc gtgggtaaag gatagtgtca
    13981 aatagccatc atacttcaat gagaggcaat gacatgagcg acaacatccg tgttgggttg
    14041 attgggtatg gttatgcgag caaaaccttc catgcgcccc tgattgcggg cacgcccggg
    14101 ctggaactgg cggtaatctc cagcagtgat gaaacaaaag taaaagccga ctggccaacg
    14161 gttacggttg tctctgagcc gaagcatctg tttaacgatc ccaacataga cctgattgtc
    14221 attcctacac ccaacgatac ccatttcccg ttagccaaag cggcgcttga ggcgggtaaa
    14281 catgtggtcg ttgataaacc ctttaccgtg acactgtcac aagcgcgaga gctggatgcg
    14341 ctggcaaaaa gcctggggcg tgtgctgtct gtattccata accgccgttg ggatagcgat
    14401 ttcttgacgc taaaaggttt gctcgcggaa ggcgtgctgg gtgaagttgc ttactttgag
    14461 tctcattttg accgcttccg tccgcaggtg cgcgatcgtt ggcgtgaaca gggcggccca
    14521 ggcagcggta tctggtacga tttagcaccg catcttcttg atcaggccat tacgctattt
    14581 ggtttaccgg tcagcatgac ggtagatttg gcacagttac ggcccggagc gcagtcgacc
    14641 gattatttcc acgccatctt gtcctatcca cagcggcgag tcattttaca cggtaccatg
    14701 ctggcagccg ctgagtcagc acggtatatc gtgcatggat cccgaggcag ttatgtgaaa
    14761 tatggcctcg atccacagga agaacgtctg aaaaatggcg agcgtctgcc gcaggaagac
    14821 tggggctacg atatgcgtga tggcgtactt acccgcgtgg aaggtgagga acgtgtcgaa
    14881 gaaacgctgt tgacggtgcc tgggaattat ccggcttact atgcggcgat tcgtgatgcg
    14941 ttaaatggcg atggtgaaaa tccggttccg gcaagccagg caatccaggt aatggagttg
    15001 attgagctgg gcatcgaatc cgccaaacat cgcgcgacgt tgtgccttgc gtgaaaaatg
    15061 gatggtgcaa tttgcaccat ccatttttgt tacttcgcgg cgactttttc tcgcagtgcg
    15121 cgtttttcct ctgcgctgag gaaagccatt tccagaccat taatctgtgc ctggcggatt
    15181 tgctcacggg ataacccagc agctggcgcg gcaacggtat attcgtgaat gatatccact
    15241 ccctgtacgc cgggatcgtc agtgttaatg ctggcacgaa tgccatgctc aaggaacgtt
    15301 ttcagcggat gtgcagccag ctctgctacg gtgctggtct gaatattgga ggtcagacag
    15361 gattcaatac caatttgttg ctcggcgaga aaatccatca gcgcccgatc ttcaatggct
    15421 tttacgccat gtccaatacg ctccgcaccc agttcacgaa tcgcctgcca gatgctttcc
    15481 ggcccggcag cttcgcctgc atggacggta atatgccagc ccgcatcacg cgcgcggttg
    15541 aagtgagaaa ggaacagact tcccgggaat ccaagttcat caccagctaa atcaagtgcg
    15601 gtaatctggt cacggtgggc taaaaaggcc tccagctctt gctgacaggc ggcttcgccg
    15661 aaggttcggc tcataatgcc gataagcttc gcctgcacac caaaggtgcg gcaaccttca
    15721 cgtacgccat cgatcaccgc ttcgacaaca cccgctacag gcagcttatg tgccattgcc
    15781 atgtagcctg gtgaaaaacg cagctcgaca tagcgcaggc cgttacgggc tgcatcttca
    15841 atgttttcaa atgccacgcg gcggcaggca tcaagagagg cgagaacttt aacgccccag
    15901 tcaagtttgg tcagaaagct caccagatcg ggttcgttgg caatgacctg aacgtgggga
    15961 atcagtgttt caagggattg tgcaggaagc gagatattat actggcggcc aagttcaaga
    16021 atggtctggg gacgaatgtt gccatcaagg tggcgatgga tatcagttaa tggcagggtg
    16081 gtatcaatca tggtcgcact ctttttctgg atataaaagt gcgcaccatt ataaaaacaa
    16141 agcagggtaa aaatccacga ttgcgcaacg attttttacg ttgcgcaatt ggggttagcg
    16201 aacagcgcgg atggcgttta ttaatccagc cacacctttt tccagtttcg aacgtgggca
    16261 gccggcattg agacggacaa aaccacgacc ttcttcacca taggtatacc ccggcatgat
    16321 cgcgactttt tcttgctcga taagcgcttt ttgcaacgcg ttgtcgtcaa tattcaacgg
    16381 acgtagatca agccaggcca gataagtgga ttgcgggatc tgccagttga gttcaggaaa
    16441 cgcggcgttc attttatctg agatatacgt caggttatct ttcagataga cgcgtaaggc
    16501 atccagccac ggtgcacctt gctgataggc ggcgatatgg gcagttaacg ccagtaccga
    16561 aggggaagaa agcccatcac gtcctttcag tgccgataaa taggcatcac ggctgctgct
    16621 attttctata atcccgtaag caccggtcag cgcgggaata ttgaaacttt tcgatcccga
    16681 tgtcagcaac gcccagtctc cccgcgccac attactccag ggaatatgcg gctgctcgcc
    16741 ccagaccata tccatatgga tttcatcgga aataacccgc acatcatgac gctcgcacag
    16801 gtcagccatg atctccagct catcgcacgt ccacactttc ccggtaggat tctgtgggtt
    16861 acacaggagc ataattttac attctggttt cgccaaaacg gcttccagct tgcccatatc
    16921 gcaaaaccag ccgtcagcct gcttctctaa agcaacgggc attactgtgc gctggttacc
    16981 ttcaatggcc ttgtaaaatg cgtcataggc gggcgtgtgg atcaccacgc cttcacctgt
    17041 ttcagaccac tgacgaatca gttctgaaac catatagatg acagaagggc catacaccac
    17101 cgtctgcgta tcaattgcgg tgtaatgctg ggtggaaaac cagtgggcaa tagccgcgag
    17161 aaactcatcg tttttccagc ggctgtagcc aaatacgccg tgcatcaggc gctgattcag
    17221 cgcctcgata atgcaggggg cagtggcaaa atccatgtct gaaatcgtga acggtaacag
    17281 gtcagcagtg ccgaaacggt cagcgacata atcccactgt gtacaccatg tgccatgacg
    17341 atccacgacc tttgaaaaat cgaacatatc ttatccttat gcctggacag tatgcatcag
    17401 accggccatt tcatctttta ctgactgaac ttgtggcccg ataacaacct gcaggttatg
    17461 ttgattaagt tgtactacgc caattgcccg attgtccttc agtgcctgca cattaacaag
    17521 cgacatatct tccacagaca aacgcagacg ggtaatgcag ttatcgaggc tgacaatatt
    17581 gtcggcaccg cctaatgctt cgaggattgc aggaacgttg taacctgatt tacccggcgc
    17641 accggcaacg gctttttcga ttgagctggc aacttcgcta tcgcgccccg gggttttcag
    17701 attgaagcgg gtgatagcga aacggaagat gacgtagtaa acgacaaacc agattgccgc
    17761 caccactggc accatgtacc acttggttga cagaccatgc aaaataccga acaccacgaa
    17821 gtcgatgata ttgccgtcgg tattaccgat ggtgacgccg agcacagaca tgacggtgaa
    17881 gccgaggccg gttaacagcg cgtggatgac atacagaact ggcgctacga acaggaacag
    17941 gaattccagc ggttcggtag tgccgccaac gacgcaggcg atcaggccag aaatcagcag
    18001 acctttaatt ttatggcgat tttccgggcg cgcgcagtga tacatagcta acgctgcacc
    18061 tggtagaccg ccgagaaacg caggcatttt accttgcgaa aggaaacgcg tgacgctttc
    18121 agaaaaaccg tgagtggtcg ggcaactcaa ttgcgcctgg aagatggtca gtgcgccgct
    18181 gacggtttga ccgcagacct cctgcgtgcc gcctgcgtcg gtaaagcgaa ttaatgccac
    18241 cagaatgtga tgcagaccaa acggcaacag cagacgttca ccggtaccaa acagcatcgg
    18301 tccgaaatca cccgcgctgt ttatcatatg gcccaagccg ctaataccca tggcgaaaat
    18361 cggccagact aatggaatca ccaggccgac aaggcccatc accagcgagg agataattgg
    18421 tacgaagcgc gtaccgccga agaatgccag cgcatccggc aggcggatat tatggaaacg
    18481 ctcatgcagc atccagacga taataccggc gatcaccgca ccgaggatcc cggtatcgat
    18541 cgactggatc ccaagaatgc tctggatgtt attggctttc agaacggcgg catctgtggt
    18601 tggcagaatg cctttattgg tcaaccagaa gtttaccgcg aggttcatta ccgcataacc
    18661 gacgaagcca gcgaatgccg ctacgccttt attttcgcgt gccaggccca gcgggatggc
    18721 gatacagaac atgacaggca ggaaactaaa agcaaacgag ccaatcttac tcatccaggt
    18781 aaagatagcc tgcaacacgg ggttgcccag gaccgggatc agggttatga catcatggct
    18841 gctaagagaa ctaccaatgc cgagcataat gccgcagaac gacaataatg ccacgggtaa
    18901 catgaaggtt ttgcctaact gctggaagaa ctcccacagc gtgactttcg gtgctgtttt
    18961 cgccgtcata aaacgactcc tcgtagagaa caatctggat tcagtaaatt gcgcgagaag
    19021 ataaaacgtt ttatcaaatt ttagtgagtc ataaatcaca ttacgcaacg ataatagcgg
    19081 gtataagata aataaaaggt aaaacgtttt atctgtcaca taatcaggga gtaggtcatc
    19141 tgcatggcta ccgccaaaaa aataaccatt catgatgttg cgctggctgc gggcgtgtcg
    19201 gtaagtaccg tttcgctggt gcttaatggc aaagggcgaa tctctaccgc cacaggagaa
    19261 cgcgttaacg ccgccattga agagctggga tttgtgcgca atcgccaggc gtcggcgctg
    19321 cgcggcgggc aaagcggcgt cattggtttg atcgtccgtg atttatctgc gccgttttac
    19381 gccgaattga cggccggatt gacggaagct ctggaagcac agggacggat ggtttttttg
    19441 cttcacggcg gtaaagacgg cgagcagctg gcacagcggt tttcactgtt actgaatcag
    19501 ggtgtcgatg gtgtggtaat tgccggggct gcaggaagca gcgatgacct gcggcggatg
    19561 gcagaagaaa aagctatccc ggtgattttc gcttcccgtg ccagttatct tgatgatgtt
    19621 gatacggttc gcccggacaa catgcaggct gcacagttgt tgacggagca tctcattcgc
    19681 aatgggcatc agcggatcgc ctggctggga gggcaaagtt cctcattaac ccgtgcagaa
    19741 cgggtggggg gctattgtgc aactctacta aaatttggcc tgccgtttca cagcgattgg
    19801 gtgttggagt gcacttccag ccagaagcaa gccgcggaag ctatcacggc gcttttacgt
    19861 cataacccga ccatcagtgc cgtggtttgc tataacgaaa ctattgcgat gggggcatgg
    19921 tttggtttgc tgaaagcagg gcggcaaagc ggggaaagcg gagtcgatcg ttactttgag
    19981 caacaggttt cgctggcggc atttaccgat gcgacaccaa ccacacttga tgatatacct
    20041 gttacctggg ccagcacgcc agcgcgggaa cttggtatca cacttgcgga tcgcatgatg
    20101 caaaaaatca cccatgaaga gacgcattca cgcaatctta ttattcccgc ccggctcatt
    20161 gcggcgaaat aatcctctct ttatctgcta tacttggtag tgtcctttcc tcaaggttaa
    20221 tgataaaaaa tgtggcacac aggcggtgcc ttttattttt actgaagttc acaggaggtt
    20281 tatgtgttta attctgacaa cctgagactc gacggaaaat gcgccatcat cacaggtgcg
    20341 ggtgcaggta ttggtaaaga aatcgccatt acattcgcga cagctggcgc atctgtggtg
    20401 gtcagtgata ttaacgccga cgcagctaac catgttgtag acgaaattca acaactggat
    20461 ggtcaggcat ttgcctggcg ttgtgatatt acttccgaac aggaactctc tgcactggca
    20521 gactttgccg tcagtaatct gggtaaagtt gatatcctgg ttaacaacgc cggtggcggt
    20581 ggtcctaaac cgtttgatat gccaatggca gattttcgcc gtgcttatga actgaatgtg
    20641 ttttcttttt tccatctgtc acaacttgtt gcgccagaaa tggaaaaaaa tggaggtggc
    20701 gttattctga ccatcacttc tatggcggca gaaaataaaa atataaacat gacttcctat
    20761 gcatcatcta aagctgcggc cagtcatctg gtcagaaata tggcatttga cctgggtgaa
    20821 aaaaatattc gggtgaatag cattgcgccg ggggcaatat taaccgatgc cctgaaatcc
    20881 gttattacac ca
//
LOCUS       DAZXUL010000068        20712 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZXUL010000068 DAZXUL010000000
VERSION     DAZXUL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20712
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.068"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACH3L1_002553"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACH3L1_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFT4077250.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACH3L1_002554"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACH3L1_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFT4077251.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LTDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACH3L1_002555"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACH3L1_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFT4077252.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACH3L1_002556"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACH3L1_002556"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFT4077253.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="ACH3L1_002557"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="ACH3L1_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFT4077254.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="ACH3L1_002558"
     CDS             complement(8217..9110)
                     /locus_tag="ACH3L1_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFT4077255.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACH3L1_002559"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACH3L1_002559"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFT4077256.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACH3L1_002560"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACH3L1_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFT4077257.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACH3L1_002561"
     CDS             complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACH3L1_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFT4077258.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACH3L1_002562"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACH3L1_002562"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFT4077259.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACH3L1_002563"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACH3L1_002563"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFT4077260.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="ACH3L1_002564"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="ACH3L1_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACH3L1_002565"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACH3L1_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFT4077261.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACH3L1_002566"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACH3L1_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFT4077262.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACH3L1_002567"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACH3L1_002567"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFT4077263.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACH3L1_002568"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACH3L1_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFT4077264.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4981 a         5727 c         5031 g         4973 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc ggtgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatcctca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag caccctacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaatgt gc
//
LOCUS       DAZXUL010000069        20693 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZXUL010000069 DAZXUL010000000
VERSION     DAZXUL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20693
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.069"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            83..352
                     /locus_tag="ACH3L1_002569"
     CDS             83..352
                     /locus_tag="ACH3L1_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFT4077265.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            346..711
                     /locus_tag="ACH3L1_002570"
     CDS             346..711
                     /locus_tag="ACH3L1_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFT4077266.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(712..1500)
                     /gene="bioH"
                     /locus_tag="ACH3L1_002571"
     CDS             complement(712..1500)
                     /gene="bioH"
                     /locus_tag="ACH3L1_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFT4077267.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1538..2221
                     /gene="gntX"
                     /locus_tag="ACH3L1_002572"
     CDS             1538..2221
                     /gene="gntX"
                     /locus_tag="ACH3L1_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFT4077268.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2280..2855
                     /gene="nfuA"
                     /locus_tag="ACH3L1_002573"
     CDS             2280..2855
                     /gene="nfuA"
                     /locus_tag="ACH3L1_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFT4077269.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3216..4532
                     /gene="gntT"
                     /locus_tag="ACH3L1_002574"
     CDS             3216..4532
                     /gene="gntT"
                     /locus_tag="ACH3L1_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFT4077270.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4577..6661)
                     /gene="malQ"
                     /locus_tag="ACH3L1_002575"
     CDS             complement(4577..6661)
                     /gene="malQ"
                     /locus_tag="ACH3L1_002575"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFT4077271.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6671..9064)
                     /gene="malP"
                     /locus_tag="ACH3L1_002576"
     CDS             complement(6671..9064)
                     /gene="malP"
                     /locus_tag="ACH3L1_002576"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFT4077272.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9676..12381
                     /gene="malT"
                     /locus_tag="ACH3L1_002577"
                     /pseudo
     CDS             9676..12381
                     /gene="malT"
                     /locus_tag="ACH3L1_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12424..13440)
                     /gene="rtcA"
                     /locus_tag="ACH3L1_002578"
     CDS             complement(12424..13440)
                     /gene="rtcA"
                     /locus_tag="ACH3L1_002578"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFT4077273.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13444..14670)
                     /gene="rtcB"
                     /locus_tag="ACH3L1_002579"
     CDS             complement(13444..14670)
                     /gene="rtcB"
                     /locus_tag="ACH3L1_002579"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFT4077274.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14851..16449
                     /gene="rtcR"
                     /locus_tag="ACH3L1_002580"
     CDS             14851..16449
                     /gene="rtcR"
                     /locus_tag="ACH3L1_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFT4077275.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16431..17189)
                     /locus_tag="ACH3L1_002581"
     CDS             complement(16431..17189)
                     /locus_tag="ACH3L1_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFT4077276.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17206..18036)
                     /gene="glpG"
                     /locus_tag="ACH3L1_002582"
     CDS             complement(17206..18036)
                     /gene="glpG"
                     /locus_tag="ACH3L1_002582"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFT4077277.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18081..18407)
                     /gene="glpE"
                     /locus_tag="ACH3L1_002583"
     CDS             complement(18081..18407)
                     /gene="glpE"
                     /locus_tag="ACH3L1_002583"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFT4077278.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18597..20102
                     /gene="glpD"
                     /locus_tag="ACH3L1_002584"
     CDS             18597..20102
                     /gene="glpD"
                     /locus_tag="ACH3L1_002584"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFT4077279.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20308..20589)
                     /locus_tag="ACH3L1_002585"
     CDS             complement(20308..20589)
                     /locus_tag="ACH3L1_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077280.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIS"
BASE COUNT         4891 a         5693 c         5217 g         4892 t
ORIGIN      
        1 gagtcagtag atactctacc ctgcttaagt gctactttgt gctatcttgc atgtaacaca
       61 aagtagcact tagggaactg atatgcagac agtgaaatta cggcaacaag gaggagccat
      121 gatcgttact atcccacgag atcttgccat cgatcttggc tggtcaccgg gcacagagct
      181 taccgtggag aaaaaaggcg atagcgttaa tttgcgcgcc acagagcaca aaccgcgtgg
      241 gcgtcttacc gtcgcgcagt tgttgagcca aatcgatgaa agcgaaatta cggagctaaa
      301 tcagtccaca gaaggatggg ctgaagggaa aaaaggtaat gaagcatggt aaaggcacgg
      361 acgccacatc gtggtgagat ctggtatttt aaccctgatc cggttgccgg gcatgaactt
      421 caggggccac attattgcat tgtggtaacg gacaaaaaac tcaacaatgt tttaaaagtt
      481 gctatgtgct gcccgatttc aacaggggca aatgcagcac gttccacagg ggtgacggtg
      541 aacgtcctcc cccgtgatac gcaaaccggt aacctgcatg gcgttgtact ttgtcaccag
      601 ctaaaagccg tcgatcttat tgcccgtggc gctaaatttc ataccgttgc cgatgaaaaa
      661 ttgattagtg aagttatcag taaactggtg aatttaatcg acccacaata attagctctc
      721 acttaccacc aacaccctct gcttcaacgc caccagcagg tgacaaaact cgaccggatg
      781 cgaaataaac ggcgcatggg ccgctttggc gaagatatat gattcgctgt gaggccaaag
      841 tttatccagc atcggcacca ctttgcgcgg caccagaccg tcgagatagc catacaatcg
      901 caaaaacggc atggacacgt tttgcagcgg ctgacggaga tcgaccgttt tcaggatttc
      961 cagcccgcca ttaagcacgt caacctccgg catcggtaac gccagaacgg ttttcttcaa
     1021 cgcccgcgca tcctggcgcg ccgtttcagt ccccatggtt tgtaacgcca ggaaccgctc
     1081 cactgtacgc tgaaaatcat cactgagttg ctgctgaaat cccgccagca cgtccggttt
     1141 tatccccggc cactcgtcac gagcactaaa acaaggtgac gacgccacgg tgaccagcgc
     1201 ctgaacacgc tcgggatggg ttaacgcaat ctggcttgcc accagcccgc ccagactcca
     1261 gcctaaccaa atggctttat caggtgcctg ttgcagcacg gcttcggcca tatcagcaag
     1321 tgacagcgca ccaaatcccc ggctacgccc gaagccgggc aggtcaacaa ggtgcagcgt
     1381 aaaatgcgag ctaagttcct cgtcaatgca acgccacact tcggcattca gtccccatcc
     1441 gtgcagcagc acaagatgaa cattcccctg acctttggtc tgccaccaga tgttattcat
     1501 ccgctattgt tctcttttga cttacaagga tgaacatatg ctaacagtac cgggattatg
     1561 ctggctatgc cgaatgccac tggcgttagg tcattggggg atttgttcgg tctgttcacg
     1621 cgccacccgc acagataaaa cgttatgccc acaatgtgga ttacccgcca cacactccca
     1681 tcttccctgc ggtcgctgcc tgcaaaaacc gccgccctgg caaagactgg tcacggttgc
     1741 cgactatgcg ccgccgttaa gtccgcttat ccaccagctt aaattttccc ggcgcagtga
     1801 aatcgccagc gccctgtcac gtctgttact attggaagtc ttacacgctc gtcgcaccac
     1861 cgggttgcaa ttgccggatc gcatcatcag cgttccgtta tggcagcggc gtcactggcg
     1921 tcggggattt aatcagagcg atttgctgtg tcagccgtta tcacgctggt tgcactgcca
     1981 atgggatagc gaagccgtca cacgtacacg ggccactgcg acccagcatt ttctcagtgc
     2041 ccggctgcgc aagcgcaacc tgaaaaatgc ctttcgtctt gaattgcccg tgcaaggtcg
     2101 ccatatggtg attgtggatg atgtcgttac caccggaagt accgtcgcag agattgcgca
     2161 gttgctttta cgcaatggtg cggcgactgt ccaggtctgg tgcctttgtc gaaccttgta
     2221 gagcctcgat gatgggcgta ttataaccaa ctaaaatagt caactattag gccattacta
     2281 tgatccgtat ttccgatgct gcacaagcgc actttgccaa actgctggca aatcaggaag
     2341 aagggacaca aatccgcgta tttgtgatta accctggcac gcctaacgct gaatgtggtg
     2401 tttcttattg tccgccggat gccgtggaag ccaccgacac tgccctgaaa tttgacctgc
     2461 tgaccgcgta tgttgatgag ttaagcgcac catacctgga agatgcagag atcgactttg
     2521 ttaccgacca gttgggttct cagttaacgc tgaaagcccc gaacgccaaa atgcgtaaag
     2581 tggcagacga tgcaccgctg atggagcgcg tggagtatat gctgcagtcg cagatcaacc
     2641 cacagcttgc tggtcacggt ggtcgcgttt cgctgatgga aatcaccgaa gacggttacg
     2701 ccattctgca atttggcggc ggctgtaacg gttgttccat ggtcgatgtg acgctgaaag
     2761 aagggatcga gaagcagctg ctgaacgaat tcccggagct gaaaggtgtg cgcgatctca
     2821 ccgaacacca gcgcggcgaa cactcctact actaagttat cccctctttt gtagattgcc
     2881 cgatgcgacg ctaaagcgtc atttcgggcc tacgttgaac atgtgccaga cgttggcaca
     2941 gcattgcttt ggcaaatccc tcccttcgtt tccccgcgat aatatgacca acctctcata
     3001 atttaaattt accccgctct ggtgattctc aaacgccaaa tgttacccgt atcattcaca
     3061 tgggtaccaa acatactcct gacatctgac tacaataatt agttttagtg ggtatcagtc
     3121 gtggtgccgc aatatctctg ttcccgattg ggataattag agtttgtcgt cagaaaattg
     3181 acgttaccca taacaaatga aaggccaggt aaatcatgcc attagtcatt gttgctatcg
     3241 gtgtaatctt gttgttgctc ctgatgatcc gcttcaaaat gaacggcttc atcgctctcg
     3301 tcctcgtggc gcttgctgtt ggattaatgc aaggaatgcc gctggataaa gttattggct
     3361 ccatcaaagc cggtgtcggc gggacgctcg gtagccttgc cctgatcatg ggttttggcg
     3421 caatgctggg caaaatgctg gcagactgcg gtggcgcaca acgtatcgcc accacgctga
     3481 ttgccaaatt tggtaaaaag cacatccagt gggcggtggt gttaaccggt tttaccgttg
     3541 gttttgccct gttctatgaa gtgggcttcg tgctgatgct gccgctggtg tttaccatcg
     3601 cggcttccgc gaatattcca ttgctgtatg ttggtgtacc aatggcggct gcactgtctg
     3661 tgacccacgg cttcctgcca ccgcatccgg gcccgactgc gattgccacc attttcaatg
     3721 ccgatatggg taaaaccctg ctgtacggta ctattctggc aatcccgacc gtgattctcg
     3781 ccggtccggt ttacgctcgc gtgttgaaag gtatcgataa gccaatcccg gaaggtctct
     3841 acagcgcgaa aaccttcagc gaagaagaga tgccgagctt tggcgtcagc gtctggacct
     3901 ctctggtgcc ggttgtgctg atggcgatgc gtgcgattgc cgaaatgatc ctgccgaaag
     3961 gtcacgcttt cctgccggta gcggagttcc tcggtgaccc ggtaatggca acgctgattg
     4021 ccgtgctgat tgcgatgttc acctttggtc tgaaccgtgg tcgttcaatg gatcagatta
     4081 acgacacgct ggtttcttcc atcaaaatca ttgcgatgat gctgttgatc atcggtggtg
     4141 gcggtgcgtt caagcaggtg ctggtagaca gcggcgtgga caaatacatt gcttccatga
     4201 tgcacgaaac caacatttct ccgctgctga tggcctggtc gattgctgcc gtactgcgta
     4261 tcgcgctggg ttccgcaacc gttgcggcaa tcactgcggg tggtatcgcg gcaccgctga
     4321 ttgcaacgac gggtgttagc ccagagctga tggttattgc ggtgggttcc ggtagtgtga
     4381 ttttctctca tgtgaacgat ccgggcttct ggctgttcaa agagtacttt aacctgacta
     4441 tcggcgagac catcaaatcc tggtcgatgc tggaaaccat tatctcggtg tgcggtctgg
     4501 taggctgtct gctgctgaat atggtgattt gataacactg actgccggat gcatcgcatc
     4561 cgacatcaac cgcactctac ttcttcttcg ctgcagctct gcgccgtctg tccaaatcct
     4621 tcagcaactt gttcacgcca tcatcggcaa acatcgactc aagcgttgcg gaaagcttgc
     4681 gtcgccagtt tttatactgg taactggtgc caggaatatt caccggttcg gccatatcca
     4741 gccagtcttc cggctgtagt cctaacagag cactgttact gtcggcaatg tagcgctgca
     4801 aaccacggtt cagcgtcggc gtcatcgaca tcaacgatgc cttatgcccg gcacgttttg
     4861 gcagacaacc atatttatgc agtgcatcca gcagcccttg cttcgccagt tcgcgatcct
     4921 gatacagacc gcgcagtacc acttcatccg gatacagccc cagggttttg cccagcgtta
     4981 gatccccgct ctcccagtaa ccacgcagcg ttggcaggtc atgtgtcgcc gcaaccgcca
     5041 tcgactgctc cggatacgct ttcggtgcac ggaacgtctt ctcgtggtcg ttttcgaaat
     5101 agagcacttt gtaagagtac acaccgctgc tgcgcagctt accaacaatc tctaccggta
     5161 cggtaccgag atcttcacca atcaccatac agcgatgacg tttactttcg agtgccagaa
     5221 tcgagagcag atcatccacc ggatagtgaa catacgcgcc ctgatctgcc gtctcgccat
     5281 acggtatcca ccacaaacgc agcatcgaca tcacatggtc aattcgtaat gcgccgcagt
     5341 tttgcatatt ggcacgcaac agctcgataa acggttcata ggcacgcgcg gtgatgatat
     5401 gcgggtccat tggcggtaat ccccagttct gccccaacgg gccgaggata tccggcggcg
     5461 cgccaaccga tgctttcagg caatatagtt cacggtcaca ccaggtttcc gccccacctt
     5521 ccgctacgcc aaccgccaga tcacgataca agccaatcgg catttcatag ccctggctta
     5581 tctcccagca ggcggcaaac tggctgtaag ccagccactg caaccagaga taaaaatcga
     5641 cgtcatcacg atgttcttcg cagaactgac gcacttctgg tgaatccacg ttcttataca
     5701 tctctggcca tgcaggccag ccccagcgca tttcgtcctc tttcacttgc tgggcatgta
     5761 gcgcatcaaa ggcagcctgc cagaacaggc tgtcgccctg ctctgcaaca aactggcgaa
     5821 acgcggccat ctgctcatca tcacgttgcg cgaaaccttt ccacgccatt cgtaatgctg
     5881 tcatttttag ggcggtaacc gtggagtaat cgacccagtc ggcatcacgc gcctgttgca
     5941 gcgtctgttg cgtggtcggc aactgccacc aggcctgagc ctcttcgcta agatggaaat
     6001 cttcaacggc gttaacgtcg atataaatca cattcagcca acggcgagaa gacgggctgt
     6061 atgggctggc gctctccgga tttgccggat agagcgcatg aatcgggttc aggccaatga
     6121 acgacccgcc acgttttgcc acatccacca gcatcgcttt gagatcgcca aaatccccaa
     6181 taccccagtt tttttccgat cgcagcgtat aaagctgaac gcaggcaccc cacagctttt
     6241 gtttattcag caaagcctgc ggttcgtaac aacgtttcgg ggcgacaatc acccggcaat
     6301 gcgcgcgctg gtcgtcctgg gtgagtgtta gcgtgtgata accttccggc agcttcgtcg
     6361 gtagattgaa cgctttgccc cccgttacat ggcctttgta ctgcgttcct tcttcggtgg
     6421 tcagctgcca gctatattcg ccgctgccct ccaccaccat cggcattttt ttgccgctgg
     6481 tataaaccat gacattcggg actggcgtta ccgccacttt cgtggcggta cgttgatgca
     6541 tcgcgtcaag caaacgccgt ttggtttcgg cgctaatcga ctgcggttta ccgtgggcat
     6601 tgatgtaatt ggggctaatc cccgccgcca gcgcggcatt atccagacgt ttgctttcca
     6661 tcgagcttcc ttagcgtttt gcctgccaga tacgagcctg ataatcgcga atagagcgat
     6721 ccgagctaaa cataccgcag cgggcggtat tgaggatcgc cgcgcgagtc caggcctcct
     6781 ggtcgcggta cagcacatcc acctgctttt gtgcctcaac ataggctgcg aaatccgcca
     6841 tcaccagata cggatcgccg ccctgtttgc cgatactgtg cagcatctgg tcgaaggcat
     6901 gcttatcgcc gtcgctgtat ttaccgcttt ccagctcttt caatactgcg tccagcacct
     6961 tatctttctt ccgccatttc accgggtcgt agcctttggc cagaatagcc ttcacttgtt
     7021 ccacggtatg accaaaaata aagatatttt cttcaccgac tttctcggcg atttcaacgt
     7081 tcgcaccatc cagcgtaccg acagtaagcg caccattgag cgccagtttc atattgccgg
     7141 taccggaagc ttctttacct gcagtcgaaa tttgttcgga gatatccgcc gccgggatca
     7201 gtttttccgc cgccgaaacg caataatccg gcaggaacac caccttcaac ttatcgccaa
     7261 ccagtggatc gttgttgatc acgtcagcca ctttgttgat cgcaaagata atattcttcg
     7321 ccaggtagta gcccggtgcc gctttcgcgc cgaagaggaa gacgcgcggt acgcgatcag
     7381 cctgcaggtt ttcacgaatt tctttgtaca acgccagaat atgcagcaga ttcaggtgct
     7441 ggcgtttgta ctcatgcaga cgtttgatct gaatatcgaa aatcgcctgt ggattgatct
     7501 caataccggt acgaactttc acaaactccg ccagacagac tttattcgcc tgcttgatct
     7561 cgcgatattg ctgacggaat ttcgcatcat cagcgaattt ttccagattg atcagctgat
     7621 cgagatcgtt agcccactct ttttgcagtg atttatccaa cagagccgcc agtgcagggt
     7681 tgcactgttt gatccagcga cgtggggtaa taccgttggt aacgttatgg aatttgttcg
     7741 gccatagctg gtgatattcc gggaacagat ctttcaccac cagatccgag tgcagcgccg
     7801 caacaccgtt caccgcgaaa ccgccaacca cacacaggtt cgccatatgc acttgtttat
     7861 cgtgcaccac cgccagtttg gcccacactt tttcatcgcc cggccaggtt ttctccacca
     7921 gcgttttaaa gcgagtatta atttcgttaa taatctgcat atggcgcggc agtaagcctt
     7981 tcaccagttt cacatcccag cgttccagcg cttctggcat cagggtatgg ttggtgtaag
     8041 cgaaagtttt gctggtgatg gcccaggcgt catcccagct catctggtgc tcatcggtca
     8101 gcacgcgcag cagttctgga atcgcgatag ttggatgggt atcgttcagc tgaataactt
     8161 cgtaatccgc cagttcgtgc agtttacgcc ccgccagatg atggcgacgc aaaatatccg
     8221 ctaccgaaca ggcacactgg aagtattgct gcatcaggcg cagcttttta ccggcagtat
     8281 ggttgtcgtt tggatagaga actttggtca gtttttccgc attgatgcct tgctgttcgg
     8341 cgcgcaggaa atcaccgtcg ttaaatttag ttagatcaaa cggatgcgca tgcgtcgcct
     8401 gccacagacg cagcggctgc gccacgccat tacgatagcc gacaacgggg agatcccacg
     8461 cttgaccggt aatggtaaac tccggctccc agcgtccgtc tttcatcact ttaccgccaa
     8521 tccctacctg cacatccagt gcttcgttgt ggcggaacca cgggtagtta ctgcgatgcc
     8581 agtcatccgg cgcttcaacc tgtttgccat cgacaaaaga ctggcggaac aaaccatatt
     8641 gatagttcag accgtaaccc gtcgcagact gaccgacagt tgccattgag tcgaggaagc
     8701 acgccgccag acgtcccaga ccaccgttac ccagcgccgg gtcgatctct tcttccagca
     8761 ggtcagtcag gttgatgtca taagccttca acgaatcctg tacatcctga taccagccga
     8821 gattcaacag gttgttgccc gtcagacgac caatcaaaaa ctccattgag atgtagttaa
     8881 catgtcgctg attcgccacc ggtttggcga atggctgagc acgcagcatt tcggccagtg
     8941 cttcactcac tgccagccac cactggcgag gagtcatttc agccgcagaa tttaagccat
     9001 aacgctgcca ctgacgtgaa agcacttcct gaaattgctt atcgttaaaa ataggttgtg
     9061 acataggagt tccacttttc ttagattttc aacacaacgt tatcgctagt ttgccaggct
     9121 cgatgttgac cttcctcatc ctgcggggga ttaggcaggg aggagttgcg gggatgagca
     9181 aggaaatgtg atctcgacca cttaaagcta gtgcaaacca caggattagc agcaaatcaa
     9241 tgccatacag cgcagaaaat ctgtatctaa gtgcaaaaaa tggccgttgc gtattttcaa
     9301 aaagcggaag gtaactctat aaattaagta aaggagtgaa acagtttcac aagtaaaata
     9361 tccagtgtgc tccatctcat tcttaataga tttattaaga tcatcttttt agatggcact
     9421 ttcatcagga atgaagaaga aacccttgct taaatgaatc tgatgaacat aagggaaacc
     9481 agtattcacg ctggatcagc gtcgttttag gtgagttgtt aataaagatt tggaattgtg
     9541 acacagtgca aattcagaca cataaaaaaa cgtcatcgct tgcattagaa aggtttctgg
     9601 ccgaccttat aaccattaat tacgaagcgc aaaaaaaata atatttcctc attttccaca
     9661 gtgaagtgat taactatgct gattccgtca aaactaagtc gtccggttcg actcgaccat
     9721 accgtggttc gtgagcgcct gctggctaaa ctttccggcg cgaacaactt ccggctggcg
     9781 ctgatcacga gtcctgcggg ctacggaaag accaccctca tttcccagtg ggcggcaggc
     9841 aaaaacgata tcggctggta ctcgctggat gaaggtgata accagcaaga gcgtttcgcc
     9901 agctatctca ttgccgccgt gcagcaggca accaacggtc actgtgcgat atgtgagacg
     9961 atggagcaaa aacggcaata tgccagcctg acgtcactct tcgcccagct tttcattgag
    10021 ctggcggaat ggcatagccc actttatctg gtcatcgatg actatcatct gatcactaat
    10081 ccagtgatcc acgagtcaat gcgcttcttt attcgccatc aaccagaaaa tctcaccctg
    10141 gtggtgttgt cacgcaacct tccgcaactg ggcattgcca atctgcgtgt tcgtgaccaa
    10201 ctgctggaaa ttggcagtca gcaactggca tttacccatc aggaagcgaa gcagtttttt
    10261 gattgccgtc tgtcatcgcc gattgaagcc gcagaaagca gtcggatttg cgatgacgtt
    10321 tccggttggg cgacggcact atagctaatc gccctctccg cccggcagaa tacccactca
    10381 gcccataagt cggcacgccg cctggcggga atcaatgcca gccatctttc ggattatctg
    10441 gtcgatgagg ttttggataa cgtcgatctc gcaacgcgcc attttctgtt gaaaagcgcc
    10501 attttgcgct caatgaacga tgccctcatc acccgtgtga ccggcgaaga aaacgggcaa
    10561 atgcgtctcg aagagattga gcgtcagggg ctgtttttac agcggatgga tgataccggc
    10621 gagtggttct gctatcaccc gctgtttggc aacttcctgc gccagcgctg ccagtgggaa
    10681 ctggcggcgg agctgccgga aatccaccgt gccgccgcag aaagctggat ggcccaggga
    10741 tttcccagcg aagcgattca tcatgcgctg gcggcaggcg atgcgctgat gctgcgcgat
    10801 attctgctta atcacgcctg gagtttgttc aaccatagcg aactgtcgct gctggaagag
    10861 tcgcttaagg ccctgccgtg ggacagtttg ctggaaaatc cgcagttagt gttattgcag
    10921 gcgtggctaa tgcaaagcca acatcgctat ggcgaagtta acaccctgct agcccgtgct
    10981 gaacatgaaa tcaaggacat cagagaaggc accatgcatg cagaatttaa cgctctgcgc
    11041 gcccaggtgg cgattaacga tggtaatcag gatgaagcgg aacggctggc aaaactggca
    11101 ctggaagagc tgccgccggg ctggttctat agccgcattg tggcaacctc ggtgctgggt
    11161 gaagtgctgc actgcaaagg cgaattgacc cgctcactgg cgctagtgca gcaaaccgaa
    11221 cagatggcac gccagcacga cgtctggcac tacgccttat ggagtttaat tcagcaaagt
    11281 gaaattctgt ttgcccaagg gttcctgcaa accgcgtggg aaacgcagga aaaagcgttc
    11341 cagctgatca acgagcagca tctggaacag ctgccaatgc atgagtttct ggtgcgcatc
    11401 cgagcacaac tgttatgggc ctgggcacgg ctggatgaag ccgaagcgtc ggcgcgtagc
    11461 gggattgaag tcttgtcgtc ttatcagcca cagcaacagc ttcagtgcct ggcaatgttg
    11521 attcaatgct cgctggcccg tggtgattta gataacgccc gtagccagtt gaaccgtctg
    11581 gaaaacctgc tggggaatgg caaatatcac agcgactgga tctctaacgc caacaaagtc
    11641 cgggtgattt actggcaaat gaccggcgat aaagccgccg ctgccaactg gttgcgtcat
    11701 acggctaaac cggagtttgc gaacaaccac ttcctgcaag gtcaatggcg caacattgcc
    11761 cgcgcacaaa tcttgctggg cgagtttgaa cctgcggaaa ttgttctcga agaactcaat
    11821 gaaaatgccc gcagtctgcg gttgatgagc gatctcaacc gtaacctgtt gctgcttaat
    11881 caactgtact ggcaggccgg acgtaaaagt gacgcccagc gcgtgttgct ggacgcatta
    11941 aaactggcga atcgcaccgg atttatcagt cattttgtca tcgaaggcga agcgatggcg
    12001 caacaactgc gccagctgat tcaacttaat acgctgccgg aactggaaca gcatcgcgcg
    12061 cagcgtattc tgcgagaaat caatcaacat catcggcata aattcgccca tttcgatgag
    12121 aatttcgttg aacgtctgct aaatcatccg gaagtgccag aactaatccg caccagcccg
    12181 ttgacccaac gtgaatggca ggtcctgggg ctgatctact ccggttacag caatgaacaa
    12241 attgcgggcg aactggaagt ggcggcaacc accatcaaga cgcatatccg caatctgtac
    12301 caaaaactcg gcgtcgccca ccgtcaggct gccgtgcagc acgcgcagaa actgctgaag
    12361 atgatgggtt atggggtgtg agtttagccg gataatgcat tggatccggt ttacatcttt
    12421 acatcattca atactcaccc gcgttacgcc atctgtttct atcaaaccaa accgcaccgg
    12481 caagaaacgc tccaccaccg cgatattggt cagcagatgg catgaaggat gggcgaccgt
    12541 aaattctccc gcgcccgcca gcgccatcgg cagtaccagt tggtcggcga gatattcccc
    12601 caccgccgct gggcttgcca ggtagcgttt cacctctttc actaactgcg cggctaccac
    12661 ctcggcactg acgcgctttt caccgacgac gaaaaagcgt tcagtgatat tttcactttc
    12721 gacttccagc gagacggtat tacccggccc ctggtcgcgc ggcaggttat gaatattctg
    12781 ttcatgcagg gaaaaaatcc ccgccagtgt agcgatttca cgctcagcaa catggcgcgg
    12841 cacaccagct aatagaactt ctccacgcat ctgcacaatg ttcccgcgct cgccaagttg
    12901 caaggtgcta aacgatgcca cgggcgagac ttccgttgcc accacaccgc ctccggcagg
    12961 ataaaaaccg tgacgtaaca gcgtggtttg ctgatgaatt cctattttcg ccagcagcgg
    13021 ctccagcacc cggcggataa aatccgcagg cggcgccgac gggttatcgg tgccgccgct
    13081 cacttcaaca cgcgaaggtc catcggcaaa ccacagcgcg ggcagcaccg tttgcagcac
    13141 cagcgtacaa cttccggcgc taccgatagc aaagcggtaa tcgccgccgc gcacggtgcc
    13201 aggccggaag accagacgct gcgatcccag ctccgcgcct tccaccgttg ccctacaaat
    13261 ttccgcagcc gcttttaccg cggtcagatg ctggcgcagc agccccggtt tcgcccgtcc
    13321 ggctcgaatg ccagtgatgg taaatggctg gccggttatc atcgacaggc tcagcgccga
    13381 gcgcaggatc tgcccgccgc cttcgccctg tgcgccatcc agcgcaatca tccttttcat
    13441 catttatcct tttacgcaca ccacctgacg cagggtgtag ataacttcca ccagatcgct
    13501 ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc
    13561 ggcatcttta cggcattcca catgcgcggt ggcacgaatt tggtcttcca cgctgaacag
    13621 ttttttcgct ttagttcggc tcattacccg cccggcaccg tggctgcacg aacagaacga
    13681 ctcttcattt cccagcccac ggacgataaa gctttttgct cccatcgaac cgggaataat
    13741 tccatattga ccagcacgcg cagacaccgc acctttacgc gtcacgtaga tctcttcacc
    13801 aaagagctgt tctttttgca catagttgtg gtgacagttg atctcttcca tcgccagcgt
    13861 ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaac gccgttacca cattttccat
    13921 catcgcatcg cggttaaggc tggcgaaaag ctgcgcccag gccacggctc ttaggtaatc
    13981 atcaaagtat tccgtacctt ccataaagta cgccagatca cgcgacggca acgtctcaag
    14041 ttggtcctgc atctcttttt gcgccagatc gataaagtaa gtcccgatgg cgttaccaat
    14101 tccgcgtgaa ccggagtgca gcataatcca cacctgctcc gactcatcaa ggcagatttc
    14161 aataaagtgg ttacccgttc ccagcgttcc caggtgttta tagttgttag tgttaaggaa
    14221 acgcggatat ttttgcgtta accactgata accggcctcc agctcagccc atttcgcatc
    14281 gacgttaaaa ggcgggtttt cccatgcgcc tttatcacgt ttacaacgcc cggtagtacg
    14341 cccgtgtggt acggccgttt caatcgcttg acgcagctcc gccaggtttt caggcaggtc
    14401 tgccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca cgcccaccgc
    14461 cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac ctttacccag
    14521 gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat
    14581 aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc
    14641 attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt gtttgcggtc
    14701 ctgataattg caaccgtcgt gccagaaaat taaataagca gattaattta ttgtttctaa
    14761 aaagattata tctttatatc cgtaaacgga gatttcccgc aaagccgatt taccgataat
    14821 gaaatatcgc cttttataag gatatctaag atgcgtaaaa cagtggcttt tggctttgtc
    14881 ggtaccgtgc tggattatgc cggacgcggc agtcagcgct ggtcaaaatg gcgtccgtca
    14941 ctctgtatat accagcaaga atcgttggtc atcgatcgac tggaattatt gcacgatgcc
    15001 cgctcgcgct cgctatttga aacgcttaaa cgcgatatcg ccagcgtttc gccagaaaca
    15061 gaagtggtgg gcgttgagat tgaactgcat aacccgtggg atttcgaaga ggtttacgcc
    15121 tgcctgcatg atttcgcccg tggttacgag tttcagccag aaaaagaaga ctatttaatt
    15181 cacatcacca ccggcaccca cgtcgcgcag atttgctggt ttctgctggc ggaagcgcgt
    15241 tatctacccg cccggttgat acaatcttca actccgcgca aaaaagaaca gccgcgcggc
    15301 ccaggtgaag tgacgattat cgatctcgat ttaagccgtt ataacgccat cgccagccgc
    15361 tttgccgagg aacggcagca aacgctcgat ttcctcaaat ccggtattgc cacgcgtaac
    15421 ccccacttca accgcatgat tgagcagatc gaaaaagtgg cgattaaatc ccgcgcgccg
    15481 attctgctta acggcccgac cggcgcaggt aagtcatttc tggcgcgacg catcttcgag
    15541 ttaaaacagg cacggcatca gtttagcggc gcgtttgtgg aagtgaactg cgccaccctg
    15601 cgcggcgata ccgcgatgtc gacgctgttt ggtcatgtga aaggcgcgtt taccggggcg
    15661 cgggaatctc gtgaaggatt attacgcagc gccaacggcg gaatgttgtt tcttgatgag
    15721 attggcgaac tgggcgcaga cgaacaggca atgctgctga aagctattga agagaaaacc
    15781 ttttacccgt ttggcagcga tcgccaggtg agcagtgatt ttcagcttat cgccggaacg
    15841 gtgcgcgatt tgcgccagct ggttgccgaa ggcaaattcc gcgaagatct gtacgcgcgg
    15901 atcaatctct ggaccttcac cctgccgggt ctacgccagc gccaggaaga tattgaaccg
    15961 aacctggatt atgaagtgga gcgccacgcc acactcactg gtgacagcgt gcgttttaac
    16021 accgaagcgc ggcgtgcctg gctggccttt gcaacatctc cccaggcaac atggcgcggt
    16081 aactttcgcg aactttctgc cagcgtcacg cggatggcca cctttgccac tagcggacgc
    16141 atcactctgg acgtggtgga agatgagata aaccgtctgc gctataactg gcaggagagc
    16201 cgcccctccg cgcttacggc gttgctgggc gcagaggcag aaaacatcga tctcttcgac
    16261 cgtatgcaac tggaacacgt tatcgctatc tgccgccagg caaagtcgct ttctgctgcc
    16321 ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca gcgtcaatga cgcggatcgg
    16381 ctacgcaaat acctggcgcg ttttggtctg acgtgggaag ccgtgcagga tcagcacagc
    16441 tccagttgaa tatggtggtc cgtcagcacc tgcatcacgc ttactggcgg cggggcgtcg
    16501 gtgtagacgg catcaaccat gctgatgctc cccatattga ccatcgcatt acggccaaat
    16561 tttgaatggt caaccaccag cataacgtgg cgcgagttct caataatggc gcgcttggta
    16621 cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc tgtcgatgcc gctgatcccc
    16681 agaatgccga aatcaaggcg gaactgggag ataaaatcga gcgtcgcttc gccaatgatc
    16741 ccgccatcgc ggctacgtaa ttcgccaccg gcgagaataa tgcggaaatc ttcttttacc
    16801 atcaacgtgt tagcaacgtt gagattgttg gtgacaatgc gcaaattgct gtgattgagc
    16861 agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga taaacagcgt cgagccattg
    16921 gggatttgct ccgccacttt gcgggcgatg cgctcttttt cttcggtctg ggtggcttta
    16981 cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg ccgcgccgcc atgatggcgc
    17041 aggatcaggt tttgctctgc cagctcgttg aggtcgcggc gaatagtctg cgggctgacg
    17101 gagaagtgct ccaccagttc ctcggtactg acataaccct gctgtttaac cagttcgata
    17161 ataccgttgt gacgttgtgt ttgtttcatt tataaatccc tggaattatt ttcgttttcg
    17221 cgcattgagc gaatcaacaa aagccatcgc taaacccacg gctaatccgg cgatgtgtgc
    17281 tccgttcgcc atcgacatcc caaacaaatc aaaccatccg gcgacaatcc agatcagcgc
    17341 aaagataatt aacccacgtt gcaggtaaat gccactttgc ggatcgcgtt cgccacgtag
    17401 ccagacgtag cccatcagcg catacaccac gccagaaagc ccgccaaacc acggcccgct
    17461 gaatttttgc tgcatatagc cgcttaacag ggcgctgata agcgtaatga caattagctt
    17521 accgctaccg aggcgttttt ccaccgcgcc gccgagatac caccaccaaa gcaggttaaa
    17581 gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg aagtaacgcc agaactcaaa
    17641 tttcagtgtt ggatcgaatg gccaggccag ccataacatc acttcctgat cgccgagaat
    17701 ttgcatggca ataaacacca ccacgcaggc gatcatcatc acccaggtca ccggacctgc
    17761 gcgttcacgc aaggcagcaa agaaaggata acggcgataa tgcaggccac tgccggtatg
    17821 gcctgccagc cagctggccg ccagataacg cggatctgcc gggttttcga gaaaacgcgc
    17881 cagctccgcc cgcacgcgct cggcctggga ctcatccgcc agccagacat cgctttggtt
    17941 atgttgttga atcgtgagga taacaccctg cgtcgccatg taatcaacaa acgcctgcgc
    18001 cacgcggggg ttagcaaaag aggtaatcat caacatcgtt gctgtcgctt attccacaca
    18061 aaaggggaca gtataaagcg ttacgcgccg tacgccacct ctgcgggaaa ctgtcgctgc
    18121 caggcttcaa agccgccgtc aatgctatag accacatcgt agccctgttg cagcagatac
    18181 tgcgccgccc ctttgctgct attgccgtga taacacatca ccatcaccgg agtgtcaaag
    18241 tcgttatcac gcataaacgc gcccagcgtg tcgttggtta aatggaaagc ctgcaccgca
    18301 tgccccatag cgaaactctg tggatcgcga atatcgacca gcaccgcctc tttttcctgc
    18361 aacttctggt gcgcgtcggc aacgttaata cattcgaact gatccatgcg tctctctttc
    18421 tttacaaaca agtgggcaaa tttaccgcac agtttacgtc gaagcggcag ataaacgcca
    18481 taatgttata catatcactc taaaatgttt tttcaatgtt acccaaagcg cgattctttg
    18541 ctaatatgtt cgataacgaa catttatgag ctttaacgaa agtgaatgag ggcagcatgg
    18601 aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg tgctggtatc gcggcagacg
    18661 ccgctggacg cggtttatcc gtgctgatgc tggaggcgca ggatctcgct tgcgcgacct
    18721 cttccgccag ttcaaaactc attcacggtg gcctgcgcta ccttgagcac tatgaattcc
    18781 gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct gaaaatggct ccgcatatcg
    18841 ccttcccgat gcgttttcgc ctgccacatc gtccgcatct gcgcccggcg tggatgattc
    18901 gcattggtct gtttatgtac gatcatctgg gtaaacgcac cagcctgccg ggatcaactg
    18961 gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat taagcgcgga ttcgaatatt
    19021 ctgactgttg ggtagacgac gcccgtctgg tactcgccaa cgcccagatg gtggtgcgta
    19081 aaggcggcga agtgcttact cggactcgcg ccacctctgc tcgccgcgaa aacggcctgt
    19141 ggattgtgga agcggaagat atcgataccg gcaaaaaata tagctggcaa gcgcgcggcc
    19201 tggttaacgc caccggcccg tgggtgaaac agttcttcga cgaggggatg catctgcctt
    19261 cgccttatgg cattcgcctg atcaaaggca gccatattgt ggtgccgcgc gtgcataccc
    19321 aaaagcaggc ctacattctg caaaacgaag ataaacgcat tgtgttcgtg atcccgtgga
    19381 tggacgagtt ttccatcatc ggcaccaccg atgtggaata caaaggtgat ccgaaagcag
    19441 tgaagattga agagagtgaa atcaattacc tgctgaaagt gtataacgcg cactttaaaa
    19501 agcagctaag ccgtgacgat atcgtctgga cctactccgg tgtgcgtccg ctgtgtgatg
    19561 atgagtccga ctcgccgcaa gccattaccc gtgattacac ccttgatatc catgatgaaa
    19621 acggcaaagc accgctgctg tcggtattcg gcggtaagct gaccacctac cgaaaactgg
    19681 cagaacatgc gctggaaaaa ctgacgccgt attatcaggg tattggcccg gcgtggacga
    19741 aagagagcgt gctgccgggt ggtgccattg aaggcgaccg cgacgattac gccgctcgcc
    19801 tgcgccgccg ttacccgttc ctgactgaat cgctggcacg tcactacacg cgcacttacg
    19861 gcagcaacag cgagctgctg ctcggcaatg cgggaacggt aagcgatctc ggggaagatt
    19921 tcggtcatga gttctacgaa gcggaactga aatacctggt ggatcacgaa tgggtccgcc
    19981 gcaccgacga tgccctgtgg cgtcgcacaa aacaaggcat gtggctgaat gcggatcaac
    20041 aatctcgtgt gagtcagtgg ctggtggaat atacgcagca gaagttgtcg ctggcgtcgt
    20101 aaattaacgt aaggtgatca ggtcagattt caatctggcc tgagactgat gacaaacaca
    20161 aaactgcctg atgcgctacg cttatcaggc ctacgtggtt tatgcaatat attgaatttg
    20221 catggtcttg taggccagat aagacgttca cgtcgcatcc ggcatgaaca aagcgcactt
    20281 tgtcaaaaat ctaacctact ttttaattca ggaaattacc gcaaagccca cgccatcata
    20341 cccaacgtaa caaaactcag gcacgttccc cactcgcccc gagaaaaata gcattaatgc
    20401 gcccagcgtc agcataaaaa tattgagcag aaggtaaatt gttggcgtaa agagtaaatt
    20461 gcaaacctca ggaatagcgg caagcccagc ccccagcatg gcaataaccg ctgcaaaaaa
    20521 ttgcgtcagt attgggatgc cgaaaaaagt cattaccagc gaggtcaaaa tccattttct
    20581 attttgcatt attctttcca ttctttctga atggcgaaat tatactcccc gagtcccctt
    20641 gccccttctg gacacttttc cgaaatgatg gcgaaaaaaa cgggaccctt tgg
//
LOCUS       DAZXUL010000070        20542 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZXUL010000070 DAZXUL010000000
VERSION     DAZXUL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20542
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.070"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(151..1800)
                     /gene="actP"
                     /locus_tag="ACH3L1_002586"
     CDS             complement(151..1800)
                     /gene="actP"
                     /locus_tag="ACH3L1_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFT4077281.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1797..2111)
                     /locus_tag="ACH3L1_002587"
     CDS             complement(1797..2111)
                     /locus_tag="ACH3L1_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFT4077282.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2311..4269)
                     /gene="acs"
                     /locus_tag="ACH3L1_002588"
                     /pseudo
     CDS             complement(2311..4269)
                     /gene="acs"
                     /locus_tag="ACH3L1_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4662..6098
                     /gene="nrfA"
                     /locus_tag="ACH3L1_002589"
     CDS             4662..6098
                     /gene="nrfA"
                     /locus_tag="ACH3L1_002589"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFT4077283.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6143..6709
                     /gene="nrfB"
                     /locus_tag="ACH3L1_002590"
     CDS             6143..6709
                     /gene="nrfB"
                     /locus_tag="ACH3L1_002590"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFT4077284.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6706..7377
                     /gene="nrfC"
                     /locus_tag="ACH3L1_002591"
     CDS             6706..7377
                     /gene="nrfC"
                     /locus_tag="ACH3L1_002591"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFT4077285.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7374..8330
                     /gene="nrfD"
                     /locus_tag="ACH3L1_002592"
     CDS             7374..8330
                     /gene="nrfD"
                     /locus_tag="ACH3L1_002592"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFT4077286.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8350..10068
                     /locus_tag="ACH3L1_002593"
     CDS             8350..10068
                     /locus_tag="ACH3L1_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFT4077287.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSNVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10061..10444
                     /gene="nrfF"
                     /locus_tag="ACH3L1_002594"
     CDS             10061..10444
                     /gene="nrfF"
                     /locus_tag="ACH3L1_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFT4077288.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10441..11037
                     /gene="nrfG"
                     /locus_tag="ACH3L1_002595"
     CDS             10441..11037
                     /gene="nrfG"
                     /locus_tag="ACH3L1_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFT4077289.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11379..12692
                     /gene="gltP"
                     /locus_tag="ACH3L1_002596"
     CDS             11379..12692
                     /gene="gltP"
                     /locus_tag="ACH3L1_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFT4077290.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12770..13459)
                     /gene="yjcO"
                     /locus_tag="ACH3L1_002597"
     CDS             complement(12770..13459)
                     /gene="yjcO"
                     /locus_tag="ACH3L1_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFT4077291.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13553..15700)
                     /gene="fdhF"
                     /locus_tag="ACH3L1_002598"
                     /pseudo
     CDS             complement(13553..15700)
                     /gene="fdhF"
                     /locus_tag="ACH3L1_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(15898..17364)
                     /gene="mdtP"
                     /locus_tag="ACH3L1_002599"
     CDS             complement(15898..17364)
                     /gene="mdtP"
                     /locus_tag="ACH3L1_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFT4077292.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17361..19403)
                     /gene="mdtO"
                     /locus_tag="ACH3L1_002600"
     CDS             complement(17361..19403)
                     /gene="mdtO"
                     /locus_tag="ACH3L1_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFT4077293.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            complement(19403..20434)
                     /gene="mdtN"
                     /locus_tag="ACH3L1_002601"
     CDS             complement(19403..20434)
                     /gene="mdtN"
                     /locus_tag="ACH3L1_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFT4077294.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20453..>20542)
                     /locus_tag="ACH3L1_002602"
     CDS             complement(20453..>20542)
                     /locus_tag="ACH3L1_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077295.1"
                     /translation="RANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4593 a         5442 c         5636 g         4871 t
ORIGIN      
        1 aaaaggcata ttctctgcat tatcattttc tacgactgtc aaaaatcgct cattttttaa
       61 tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat gttggagagg aagcgtattc
      121 cccggtcagg cgaccgggga aggggagaga ttaatgtgcg cggccttgct caacgccaaa
      181 gccggtctgg gagcggataa actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc
      241 tgagttatcg gttgccgaga agaaccagat gccgaggaat gccacgctga tagagaacag
      301 cgccgggtat tcatacggga agatggcttt ttcgtgaccg aggatctgta cccaaatagt
      361 cgggccgagg atcatcagca ccactgcggt aatcagcccc agccagccgc ccagcatcgc
      421 gccacgggta gtcagtttcg accagtacat ggaaagcaga atgatcggga agttacagct
      481 cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg ttctgattct caaacagcac
      541 gccgaggata atcgcaatca cgccgaggat cagtacggtg atttttgata cccgcagctc
      601 ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac aagtcatgcg aaaccgccga
      661 tgcgcccgcc agcgtcagat ccgcaaccac cgcgaggata gtggcgaaag caaccgctga
      721 aataaaaccg aggaataggt tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt
      781 gttaccacca atcagatggc ccgccgcgtc tttatattcc ggattcgcac caacaagcat
      841 gatcgcgccg aagccgataa taaaggtcag aatatagaag tagcccataa atccggtggc
      901 gtagaacacg ctcttacgtg cttcgcgggc atcgctgact gtaaagaagc gcatcagaat
      961 gtgcggcaag cccgccgtac caaacatcag tcccagcccc agagagagcg cggagatcgg
     1021 atctttcacc agtccgcccg gtttcatgat gtcgacacct ttcgggtgta ccgccatcgc
     1081 ttcgctgaac agattgttga agctaaagcc gacgtgtttc atcaccataa aggccataaa
     1141 gctggcaccg aacagcaaca gcacagcttt gataatttgt acccaggtgg tcgccagcat
     1201 gccgccgaac aggacgtaca tcatcatcag cacgccgacc agcaccaccg caatgtgata
     1261 gttaaggcca aacagcagct cgatcagttt acctgcgcct accatctgag cgataaggta
     1321 aagcgccacc actaccagag aaccacaggc cgaaagaata cgaatcggcc cttgtttcag
     1381 acgataagag gccacatcgg cagaggtgta gcgtcccagg ttacgcagac gttcggcaat
     1441 cagaaacaga atgattggcc agcccaccag gaagcccaac gagtagatca gcccgtcata
     1501 gccggaggta aacaccagcg cggagatccc caagaatgag gcggcggaca tatagtcccc
     1561 ggcaatcgcc agcccgttct ggaagccagt gatattgccg cctgcggtgt agtagtcgct
     1621 acgagaacgt acgcgttttg atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat
     1681 caggaacata ataatcgcct gccagttcgt tggctggcgc tctacggccc cgctaatagc
     1741 atccgcggcg ttagctgcga aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat
     1801 gatgcttgta cctcatgcag gacttcatta ttaagacggt cgaattcgcc gttcgcccgc
     1861 cagatgtaga taccggtgag aacaaaggag atcacaatca ctccaacacc aattggaata
     1921 ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca gccagccggg cgcgaaggcg
     1981 atcagtaaaa taaagccgat ataaactgcc agcataataa tcgacaggat ggtggcaaac
     2041 cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt cttctatccg ctgataaatc
     2101 gtaccattca tcacagattc tccagaggta atgtagggat tgttttaatt cccgctccct
     2161 tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg taggccggat aaggcgttta
     2221 cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacaaac
     2281 cgttaccgat tcgcatccgg caattgtggg ttacgatggc atcgcgatag cctgcttctc
     2341 ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc gaggtatcgc ccaggttgct
     2401 ggtatcgccc gccgcaattt tgcgcagaat acggcgcata attttgccgg agcgggtttt
     2461 aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc agcgggccaa tctctttacg
     2521 cacccagttg cggacttctg cgtacagttc tggtgacggt tcctccccgt gattaagcgt
     2581 gacataggcg tagatcgcct gacctttaat attgtgcgga atacctacta cggcggcttc
     2641 ggcaatcttc ggatgcgcca ccagcgccga ctctatctct gccgtcccca gacggtgacc
     2701 ggagacgttc agcacgtcgt ccacacgccc ggtgatccag taatagccat cttcatcgcg
     2761 acgcgcgccg tcgccgctga aatacatatt tttgaaggtg gaaaaatagg tctgctcaaa
     2821 acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc caggagtcgg tgatcaccag
     2881 gctaccttcg gtagcccctt ccagcgggtt accttcgtta tcgaccagcg ccggttgcac
     2941 gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg gtagcgccag gcagcggggt
     3001 gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg accaccggac atttctcgtt
     3061 gccgattttt ttccagtacc actcccacgc ttccgggtta attggctcgc ccacggaacc
     3121 gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct ttatcgcctt ccgccatcag
     3181 cgcgcggatc gccgtgggtg cggtatagag aatattgacc tgatgcttgt ccaccacctg
     3241 cgccatacgg gcaggcgtcg gccagttcgg tacgccttca aacatcagcg tggttgcgcc
     3301 gcaggccagc gggccgtaca gcaaataact gtgtccggta acccagccca catcggcggt
     3361 gcaccagtag atatcacccg gatgataatc aaagacatat ttaaaggtca gcgccgcgta
     3421 caccagataa ccgccggtag tgtgcagtac gccttttggt tttccggtag aaccggaggt
     3481 atagagaata aacagcggat cttcggcgtt catctcttcc gcctagtgct gatcgctggc
     3541 ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc tgccagtcga tgtttccgcc
     3601 agtacgcttc agtaccacca catgctctac gctggtgacg ttcgggtttt ttagtgcgtc
     3661 atcaacgttt ttcttcagcg gaatactacg cccggcgcgc acgccttcgt cggaagtgat
     3721 caccagtcgt gagttggaat caataatgcg cccggcaacg gcttccggcg agaagccgcc
     3781 gaaaatcacc gaatgcatcg cgccaatgcg ggcgcaggcc agcatcgcaa ccgcggcttc
     3841 cggcaccatc ggcatataaa tcgccaccac atcacctttt ttaatgccca gctcgagcag
     3901 ggtattggcg aagcggcaga cgtcgcggtg cagctcttta tagctgatat gtttgctctg
     3961 gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg ccgttttctt gcagatggcg
     4021 gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg taccatttaa tggacacatt
     4081 accgggggca aaggaggtat ttttcacctt ctggtacggt ttgatccagt cgagaatttt
     4141 tccctgttcg ccccagaagg tatcaggtac gttaatagat tgttgataca tcgcctcgta
     4201 ctgctgaggg tttatcaggc aacggtctgc gatgttggca ggaatggtgt gtttgtgaat
     4261 ttggctcatg cttttgttct ccttgtagga tgttaataat atgtggcata agcgttaaat
     4321 gtaggggtat tggcagtttt gtttagtatt tgggcgacag atcacgcaaa agtagaatgg
     4381 tgcaaataaa cggcagggta atttttgaag gtcaccaaca aaagttgatt aattctttga
     4441 ggaacatgca gttatgcatg ctgttgaaaa agaggaagat actgactaac tctaaagtgg
     4501 tatttcacat gcacttacaa ttgattaaag acaacattca cagtgtggtt atttgttaca
     4561 cataggggtg agcaatgtca tgacagtgta ggtgcggtta ctgtcgtgaa aaacaataaa
     4621 aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc tatgacaagg ataaaaataa
     4681 acgcacgccg tatcttcagc ttattgattc cttttttctt tttcacttct gttcacgctg
     4741 aacaaacggc agctcccgca aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc
     4801 cgcagcatca cgatcaatat ctctcctgga aagccacctc ggaacagtca gagcgtgttg
     4861 acgccctggc ggaagatcca cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg
     4921 attacaacaa gcctcgtgga catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta
     4981 ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat ggcgtgctgg agttgtaaaa
     5041 gcccggatgt ggcgcgtctg atccagaaag acggcgaaga tggctacttc cacggtaaat
     5101 gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg tgccgactgc cataacaccg
     5161 cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc
     5221 gcgcgatgga agccattggt aaaccttttg agaaagccgg acgtttcgac cagcaatcga
     5281 tggtttgcgg tcagtgccat gtggagtatt acttcgacgg caaaaacaaa gcggttaaat
     5341 tcccgtggga tgacggcatg aaagtcgaaa atatggagca gtattacgac aaaattgcct
     5401 tctctgactg gactaactcc ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat
     5461 atgaaacctg gacagcgggc attcacggta aaaacaacgt gacctgtatc gactgccata
     5521 tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga ccataaaatt ggtaatccgt
     5581 ttgataactt cgcccagact tgtgcgaact gccataccca ggataaagct gccttgcaaa
     5641 aagtggtcgc ggaacgtaag caatcgatta acgacctgaa aatcaaggtt gaagatcaac
     5701 tggttcacgc tcacttcgaa gcgaaagcgg cgctggatgc aggcgcgacg gaagccgaaa
     5761 tgaagccgat tcaggacgat atccgtcatg cccagtggcg ttgggatctg gcgattgctt
     5821 cccacggtat tcatatgcac gcaccggaag aaggtctacg gatgctcggt acggcgatgg
     5881 ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct ggcgaccaaa ggtatcaccc
     5941 atgaaatcca gatcccggat atctcgacca aagagaaagc ccagcaggcc attggcctga
     6001 acatggaaca aatcaaggcc gagaagcagg acttcatcaa aacggtgatc ccgcagtggg
     6061 aagagcaggc acgtaaaaac ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg
     6121 cggaaaacac aatggagtga atatgagcgt attacgttcg ttattaactg ctggggtgct
     6181 ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct acccctgcgg tgcaggcatc
     6241 tggtgatcgc tacgaagtta cccagcagcg taacccggat gccgcctgtc tggactgtca
     6301 taaaccagat accgaaggta tgcatggcaa acatgcttcc gttatcaacc cgaataacaa
     6361 attgccggtc acctgcacca actgtcacgg ccaagcatca ccgcaacacc gcgaaggggt
     6421 gaaagatgtg atgcgcttta acgagccgat gtataaggtc ggagagcaga acagcgtctg
     6481 tatgtcctgt catctgccgg aacagttgca aaaagcgttc tggccgcacg atgtccacgt
     6541 aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca cagcaagata cgatgcagac
     6601 gttaagtgac aaaggacgga ttaagatttg cgtcgattgc cacagcgatc agcgcaccaa
     6661 tccgaacttt aacccggcgt ctgttccgtt gcttaaggag cagccatgac ctggtctcgt
     6721 cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg
     6781 gtggcgaaaa cgttgaatat caatggggtg cgttacggca tggtgcatga cgagtcgtta
     6841 tgcatcggct gtaccgcctg tatggatgct tgtcgggaag tgaacaaggt gccggaaggc
     6901 gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat
     6961 cgcttcttcc gtaagtcttg ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg
     7021 accggtgcgt cttttcgtga tgccgctagc gggattgtcg atgttaatcc tgatctctgc
     7081 gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc
     7141 acgaagacgg cggacaaatg cgatttctgt cgtaagacca atttgcaggc cggtaagctt
     7201 cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt ttggcaatct ggacgatcct
     7261 aacagtgaga tttcgcaact gctgcgccag aagcctactt accgctacaa gctggcgctg
     7321 ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc
     7381 agacttccgc atttcatttt gaatcgctgg tgtgggactg gccgattgcc atctacctgt
     7441 ttttgattgg tatttctgcc ggtctggtga cgctggccgt gctgttacgt cgcttctacc
     7501 cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac gctgattgtc gggccgggcg
     7561 cggtgatcct cggtctgttg atcctcgtct tccacctgac aagaccgtgg accttctgga
     7621 agctgatgtt ccactacagt tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc
     7681 tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc
     7741 tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc
     7801 ccgttcatcg cggactggaa acgctgatgc tggtgttggc ggtgctgctg ggggcttata
     7861 ccggctttct gctgtcggcg ctgaaatcgt atccgttcct caataacccg agcctgccgg
     7921 tgctgctcct cttctccggc atctcgtccg gtgcggcggt ggcgctgatc gccatggcga
     7981 tacgccaacg cagtaacccg cattccacgg aagcgcactt tgttcaccgg atggaaatcc
     8041 ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt tgtcggtctg gcgctgggcg
     8101 atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg cggtttctgg acgtggtggt
     8161 tctggcttgg tgtcgccggg ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca
     8221 atcgcagttc cggcattcct gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt
     8281 tgatgctgcg ctttttcatt ctctacgccg gacagttgac ggtggcgtaa gccagaaaag
     8341 aggtggtttc tggacgtatt ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc
     8401 ggggtcaacg tgttgacccc gttgacggcc tttgcgggag tgcggttgcg ctggcctgcc
     8461 atgatgcgac tcacttgcat cggcattctg gcgcagttcg cgctcctgct gctcgccttt
     8521 ggcgtactga cgtattgttt tctcatcagc gatttctcgg tcatttatgt cgcccaacat
     8581 agctacagcc tgctgtcgtg ggaactcaaa ctggcggcgg tgtggggcgg tcatgaaggt
     8641 tcgctgctgc tttgggtgct gctgctttcc gcctggagcg cgctgtttgc ctggcattat
     8701 cggcagcaaa gcgatccgct atttccgctg acgctagccg ttttatctct catgctcgcc
     8761 gcactgctac tgtttgtggt gctgtggtcc gatcccttcg tgcggatatt tccaccagca
     8821 atcgaaggcc gcgatctcaa tccgatgctg caacatcccg gtcttatctt tcatccaccg
     8881 ctgctttacc ttggctatgg cggtttgatg gtagcggcga gcgtggcgct ggcgagtcta
     8941 ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct ggcgctgggc gttacctggc
     9001 tggagtgcat taacggcggg gatcatcctc ggttcctggt gggcctactg cgaactcggc
     9061 tggggcggct ggtggttctg ggatccggtg gaaaacgcct ctttattacc ctggctttct
     9121 gccactgcgc tgctgcacag tttgtccctg acacgccagc gggggatttt ccgccactgg
     9181 tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc tgggcacctt aattgtccgt
     9241 tctggcattc tggtttcggt tcatgcgttc gcactggata acgtccgcgc cgtgccgttg
     9301 ttcagcctgt ttgcactgat tagccttgcg tctttggctc tgtatggctg gcgagcgcgg
     9361 gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg aaatgttaat cctcgctacg
     9421 ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg gaacgcttta tccgatgatt
     9481 tacggcctgc tgggctgggg acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg
     9541 ttaccgtttg gtctgttgat gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa
     9601 cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc tgttatttgc cgcggggatc
     9661 gtggtttcca atgtcagtcg tcaggagatc agcctgaatt tacagccggg tcagcaggtg
     9721 acgctggcag gatacacctt ccgttttgag cgactcgatc tgcaagccag aggcaattac
     9781 accagcgaaa aagcgatagt ggcgctgttt gaccatcagc aacgcattgg tgaattaacg
     9841 ccggagcggc gtttttatga agcacgccgt cagcaaatga tggaaccgtc aattcgctgg
     9901 aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa ccagagcgga tcgttacgct
     9961 tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg ggggaggatt gttgatgatt
    10021 gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg atgaataaag ggcttctcac
    10081 gctactgctg ttatttacct gttttgcgcg cgctcaggtt gtagacacct ggcaattcgc
    10141 caatccgcaa caacagcaac aggcgttaaa tattgccagc cagttacgtt gtccgcagtg
    10201 ccagaatcaa aacttactcg aatccaatgc gccggtggcg gtcagtatgc gccatcaggt
    10261 ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc attggctgga tgaccgaacg
    10321 ctacggagat tttgttcgct ataacccacc gttaacgggt cagacgctgg tgttgtgggc
    10381 gctgccagtg gtgttgttat tgctgatggc actgatcctc tggcgagtga gggctaagcg
    10441 atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac tcacgatatt gatggtattc
    10501 ctctgtgtcg gcagttatct gttaagccca aaatggcagg cggtacgtgc ggagtatcag
    10561 cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa ccccggaagc gcagcttcag
    10621 gcattgcagg ataaaatccg cgctaatcca caaaacagcg aacaatgggc gttactgggc
    10681 gagtactatc tgtggcaaaa cgattacagc aattcgctgc tggcgtatcg tcaggcgttg
    10741 caactacgtg gtgagaacgc tgaactgtat gcggcgctgg cgacggtgct ttattaccag
    10801 gccagccagc atatgaccgc ccagactcgc gcaatgatcg acaaagccct cgcgctggac
    10861 tctaacgaaa tcaccgctct gatgctgctg gcttccgatg cgtttatgca ggcgaattac
    10921 gcgcaagcca tcgaactatg gcaaaaagtg atggatctca actcaccccg gatcaaccgg
    10981 acacagctgg tcgagtcgat taatatggca aaactgttgc agcggagatc ggattaatca
    11041 tctggtgatg aagattatgt cattgagctg cataaaaaat aatcgaatga acatatgcca
    11101 aaaataatca ccaatcagta ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa
    11161 tggttattca ttaatcctgc cataaactcg cgttattatg cattaatgca gcgaaaagct
    11221 ctgttgttaa agggttgcgc aacataccgc gcaaatgata ctgatcataa gcgttaaaaa
    11281 aatctacaaa ccaacgcaac acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc
    11341 tgaacgggga acggcgctcc attgaggaag ttattctgat gaaaaatata aaattcagcc
    11401 tggcctggca gattctgttt gctatggtgc tgggcattct cctgggaagc tacctgcact
    11461 accatagcga cagccgcgac tggctggtcg tcaatttgct ctctccggcg ggtgatatct
    11521 tcatccatct gatcaaaatg attgttgtgc cgattgtgat ctccacgctg gtggtgggta
    11581 tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg cgcgaaaacc attatttact
    11641 tcgaggtgat caccaccgtc gccatcattt tggggatcac tctggcgaac gtcttccagc
    11701 ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca
    11761 ctacggaagc ggtacaaagc agttcccacg gcattatggg cacgattttg tcgctggtgc
    11821 cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct gccgatcatc tttttctcgg
    11881 tgctgtttgg tctggggctt tcttccctgc ccgcgacgca tcgtgaaccg ctggtgaccg
    11941 tgttccgctc catctctgaa accatgttta aagtgactca catggtgatg cgttatgcac
    12001 cggtgggtgt gtttgcgctg attgcggtga cggtggctaa ctttggtttc tcgtctctgt
    12061 ggccactggc gaaactggtg ctgctggtgc atttcgccat tctgttcttc gcgctggtag
    12121 tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg gatcctgatt cgtattctga
    12181 aagatgagct gattctggcg tactccactg ccagctctga aagcgtactg ccgcgaatta
    12241 ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg
    12301 gttactcttt taaccttgat ggttcgacgc tgtatcaaag tattgccgct attttcatcg
    12361 ctcagctgta tggcattgac ctgtccatct ggcaggaaat cattctggtg ctgacgctga
    12421 tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc gtttgtggtg ttgctggcaa
    12481 cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt tattgctggt gttgaccgta
    12541 tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga
    12601 ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc
    12661 tgggcaaatt tgataaaact gcggatcaat aattgaagat tgccggggat atcccccggc
    12721 aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc aggcctacct taaccgttgg
    12781 ttaatttttc aaactcttca cacccggtat caaacccttc catacagctc aggttcaacc
    12841 agtgcagcgc cttttgtttg ttcttctcga taaaaccttc ctcaccgttt aagaacatca
    12901 ttcccgccca gtactcggaa taaccggttc gggaaattgc agagctgcgt ttgaaatacc
    12961 aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt ggcgtaaatc aaaccaagca
    13021 gcatttgggc atcgacggcg gagtcattct ccagatcttc cgaggcgttt tctaacagcg
    13081 aaatcgcctt tggataatcc ggtttacccg cttgagtatt taccagaata tgcgccagcg
    13141 ttacttcacc ttctttactg cccgcttgcg ccgctttctc tgcaagaact tttgcttgtg
    13201 gatagtccag actgaccgga ttggtgattt tgatctgcgc caacagcgcg caggcatcag
    13261 catcaccgct gtcggcggct ttctgtgccc aatattcggc tttgctcaaa tcgccggagc
    13321 taaaccagct gtcggcaaga aaatattgtg cacgtcggtc cccggcctct gctgccttta
    13381 aatactggct gccaggctcg gagtcgttcg cgtgggcaaa gaatgtcaaa aacaacatca
    13441 atgcgataat ttttttcatt ttgataatac gattgggtag ggagtaacca gtataaagag
    13501 ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta gaaaggactg tattacgcca
    13561 gtgccgcttc gcgcaggcga gttttcaact tgttgtactc gtcaatcacg tactgctcgg
    13621 cggcgcgctg atcggagatc ggctcgacgc gcacggcgca gtatttgtat tccggcgttt
    13681 tcgtaatcgg gcttaagctt tcggtaacca gctcgttaca ggcaccaatc caccactggt
    13741 aggtcatgta aatcgcccct ttgttcggac gatcgctgac ctgcgcacgg gtgataattt
    13801 tgcctttacg cgagtgcact caaaccaatg cctcatcttc aatacccaga cgtttggcgt
    13861 cttcggtatt gatttgtgcg tagccaggtt catcagccag cgccgccagt gccgcacagt
    13921 taccggtcat cgaacggcaa gagtagtgac caacttcacg caccgttgac agtaccatcg
    13981 ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc gcaggtgaag aactgcgcca
    14041 gaccgttcgg ggtatcaaac ttctctttaa acagataaga agtcccctga tcggcatctg
    14101 aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc cattttctcg taagtcgcac
    14161 cgtagaaatc cgggcacaga tgacgcaact catcccagat ctcctgggtg ttgttgtagt
    14221 gcatcggata acccatacgg gtggcgattt cactgatgat ttgccagtcc gttttcagat
    14281 cccatttcgg ttcaactgct ttgaagaaac gctggaagcc acggtcagcc gcagtaaaca
    14341 cgccttcatg ctcgccccac gacgttgacg gcaaaatgac atccgccgcc gacgcggttt
    14401 tggttataaa gatgtcctga acgatgacca gttccagatc ttcaaaggct ttacgtactg
    14461 ccgacagctc cgcatcagtt tgcagcggat cttcgcccat aatgtacgcg gcacgcactt
    14521 cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata gccggtatga gcaggcaggc
    14581 tttccacgcc ccaggctttg gcgaattttt cgcggttagc cggatctttc acgtactggt
    14641 atcccggata cgtatccggc agcgcgccca tatcgcaggc accctgcacg ttgttctgac
    14701 cacgaaccgg gtttacaccc gcatgcggct taccgaggtt actggtcagc atcgcgaggc
    14761 tggtcagaga acgcacggtt tccacgccct ggtagaactg ggttacaccc atgccccaca
    14821 ggatggcggc gcttttcgcc tgggcataca tccgtgccgc ctgacgaatc tcactggcgc
    14881 tgacaccggt gatatcttca accgactccg gcgtgtagcc ttcaacgatt ttacgatact
    14941 cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc gtacagattt tcttcaataa
    15001 tgacatggcc catcgcattc aacagcgcga tgttcgagcc gtttttcagt gcaatgtgca
    15061 tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca gacgataatt ttcgccccgt
    15121 tacgtttagc gttaattacg tgattcgcca cgattgggtg ggaatccgcc gggttgtacc
    15181 cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat agcattgctc attgcgccat
    15241 taccgaccga ttggtgcaga cctgcaaccg atgggccgtg tcagacacga gcgcagcagt
    15301 caacgttatt ggtaccaata acggcgcgcg caaatttttg cattacatag ttggtttcgt
    15361 tacccgtacc acgcgaggag ccggtcgtct ggatggcatc cggaccgtac ttctctttga
    15421 tggcgctcag gcgctcggca acgtaattca gtgcctcatc tcaggaaaca ggttcgagtt
    15481 tgccgccacg ctggcgacgg atcatggggg ttttcaggcg cggggtcagg atctgggtat
    15541 cgttaatgaa atcccagcca taataaccct tcagacacag ggtaccctgg ttggttttcc
    15601 cctgcgctgc ctccgcccgg acgattttgc cgttatcgac gaccaggttg attttgcaac
    15661 ctgatgcgca ataggggcaa accgtgacga cttttttcat cggtctcgct ccagttaatc
    15721 aaatcacgca tacgcgctct cgactacagt atgcatcttt tatgccacat tttatttggg
    15781 gtcattccct gatattacgg gcactattta ttcaaaactc tgacgaaaaa caggctgtcg
    15841 tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc atcacgcggc agacatttta
    15901 ttttttctcg acgacgggag ccgcctgata cccgccgccc agcgatttca tcaactgaat
    15961 gctttggatc acccggcggc tgtccagcat cagtaatgac atctcttcgg caagtactgg
    16021 caaccgggct tcagtggcct gtaagcggct ggttaagccg cgctgatagg cggcctcggc
    16081 agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc tgcatttctc gctcgtcgtt
    16141 gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc accgcgttca gtactgactg
    16201 gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg ccttcgagat tggcattcaa
    16261 ccgtccaccg tcaaacagcg gcaatttcag acccgggatg aagttgaact ggcgactggt
    16321 ttttttgaat aaggtatcca ggtggatggc atcaaggccg aaaaacgctt tgatatcaaa
    16381 gctcggatag aacaacgccc gcgcggaatc cacctgatct aatgacgcct gaacatacca
    16441 gcgcatggct tgcagatccg ggcgtctggc gagcaactca taagagagtg tcgccggaat
    16501 gccggtctgg actcgtggta atgccacagg tttgatctcc ggcatatcgc tggctcccgc
    16561 gccaatcaat gcgcgcagag attctctcgt ttcggtgatt tgccctttga cggcagcaat
    16621 ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg aaaggcactt gtgcttccag
    16681 accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa tcaatcacat cgcgagtttg
    16741 ttctaacaga tcgagcatct gatagctggc ctgcatactg taataaagct gcgctacgcc
    16801 cgtggtcagc gatagctcta ctgctgcggt ttctgccagc gcggcattat gcgcgccaat
    16861 ggcggcggca accgctgagc gatgcacacc ccacaaatca agatccagtc cggcaaacaa
    16921 acctactgtg gcttccgtat agtacggccc gtccatcccc agtgcgggcg catccatcgc
    16981 ataagggctt aaaaagccgt tcgccgagac acgttggcgg ttaagcatcc ctaacgctgc
    17041 cacctgcaac tgtgaaccgg catctaacaa atcggcctgc gattgcgctt tttcttcccg
    17101 cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc cgttggatca gggcatccaa
    17161 ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc cagccggagc tggcaagatg
    17221 aatatcatcg gccagtttga tttgttccgg tttgagctgt tgatgaggcg cagaatcctt
    17281 acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg agaatgctgc acaacagcag
    17341 acgtgaaagt tgacgattga tcattgcgtg gctccttgtg cctgttccgt ggcgggcgtt
    17401 aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct ggcagacgtg ttgctcctgt
    17461 tttaataagg tgggcaggat ggcctgcgag gtgggtgtct cttcaagcgt tatttgagga
    17521 gaacggctga gtgcggttgc cagaccggca gcgtatttct ccagggcgtc ggcaaactga
    17581 cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg ccgaattcca ggtggcatcc
    17641 caggcgtgaa ggatatcgcg gccctgacga ataaccgttt gcgaacgttc aatcagtaag
    17701 gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc gttggcacat ctcttcacag
    17761 gcattaaacg ccgcatgcag accgatacga atttgcagat aagtgcgcag agccgtgact
    17821 tcctgctggc gtgggatccg cattacttta cttaacatac ccagcgcgcc agccagtttt
    17881 tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg tgtaaatcat cgcggacacc
    17941 acggtaccaa tgatgatacc cagggcgcga tcgcgaattt ccaccaggtc gtacactggg
    18001 ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca ccatctgtgt gccgatataa
    18061 gaagagcgtt cagagctggt ggcaatccat gcgcccaaca ggaaaatcgg tgccagcaca
    18121 aacagcaatt cgacaatatt gtccagccag ggcatgacca gtagcgtgaa taacagcgcc
    18181 agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca tcttctggta cgacgaaccg
    18241 acatttggat tagcgacgat cacgcaggtc agcatacagg tgtgaatgcc ttcccagtcc
    18301 acgccgctgt aaaaggtgta acagatcaaa caggcgagca atgtttttac cgcgtagcgt
    18361 atatagtctg gattggtaaa agcatcggcg accattgatg gcggtttggc ggcgggcgtt
    18421 ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg tctggcagat attctccagg
    18481 ttacattccc gtgccgccac cgcttcactt tcactgagcc gccagtcgct ttgccagcac
    18541 tgaccttcag caacggcatg ctgcagcttg ttgatttctg aggctaattt ttgccgaaat
    18601 tcaataattg cctgagaatc agcaaaagag gtgggatcgt agcgattcag cgtcgagtaa
    18661 atgtaggtta ccgttgccac gcagctttgc caccatgcgc tttgagttcg ccagttggca
    18721 tcgtccgcga gacaaaagac attcagtttt tgcagcgcca gcgcctctct ttcaatccgc
    18781 gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa tggcatcatc aagccgatca
    18841 ttaagcgctt gatgcatttg cgtaatggca cgattgggaa accacagcac gccgattaac
    18901 gtcatcagca aggttggata gaggccaaca acgatacacc acagcgttaa gcgcacgact
    18961 acttccggat agtcgagcat ggcggggaag gtttgcccgt aaatggcgac agcgaaaaag
    19021 accagtccca gccgatgggt gcgcatcaaa aacatgcagc ccatcaggat cggcccggcg
    19081 atgatcaatc ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg
    19141 atctccagca ccgtggcaac cacaaacaag atcgccacaa atttggtgta aaacgcgttc
    19201 gactgaatac cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc
    19261 atcgagatca gaatcaccag caggcagcct acccagagtt gcacgatctg cggcacgcgg
    19321 ccgggtcgcc gctcgcttaa ctcttcatga aagaacgcca gcagcctgac caccggtaat
    19381 ggcagggagt tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga
    19441 tgcggaacat ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa
    19501 cgcggaccca gttaatagaa cgagacactt ttggcaggcc gcccagcacc aggccgccgt
    19561 catccggtag cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt
    19621 cactcatcag gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc
    19681 ggaagttggc gatcacgtac cagtgacgag tgtcgatgag ggtaaaaata gggcgcatgg
    19741 cagaagcaaa ttgcccgacg gaggttttga gggaaatgat ccggccatca aatggcgcgc
    19801 gaacggtcgt catttccaga tgtagtttgg tcagggcaat atccgcttcg accgccgcac
    19861 gttgcgccac cagcgcatcg acgccgctga cggcgctggc ggctgactgc gcctgtaaca
    19921 atacggcatt aagatccgct tctgcggcgc gctgcgccgt tcttgcacgg tcaacgtctt
    19981 ctgctgagac aaaaccctct ttcagtaatg gctcggtgcg gcgtaatgta tctgtggcct
    20041 gtttcgcggc ggcacgggct ttttctaccg tggcattaac cgagtcggca ccaaactgtt
    20101 gggcgtcaac gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt
    20161 cggctttcgc caggttggct tcgtacgggc gcgggtcgat gcggaacagc aaatcgccct
    20221 gtttcaccgc ctggttgtcg gtgaccgcca gttctacaat gcgaccgctg acttccggca
    20281 ccacatcaat ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc
    20341 gccagataac gaaaacaagg gcaaccagcg ccaacgcaac cactaacaga gcagggaatt
    20401 tactgcgagg tgcttttttc ggcgtacttt ccatgagcgc gtctcaaata gattagaaaa
    20461 atgccagcca gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa
    20521 atgccagatt gatatttgcc cg
//
LOCUS       DAZXUL010000071        20521 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZXUL010000071 DAZXUL010000000
VERSION     DAZXUL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.071"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="ACH3L1_002603"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="ACH3L1_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFT4077296.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="ACH3L1_002604"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="ACH3L1_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFT4077297.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="ACH3L1_002605"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="ACH3L1_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFT4077298.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACH3L1_002606"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACH3L1_002606"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFT4077299.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACH3L1_002607"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACH3L1_002607"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFT4077300.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="ACH3L1_002608"
     CDS             4449..4814
                     /locus_tag="ACH3L1_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFT4077301.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="ACH3L1_002609"
     CDS             4964..5482
                     /locus_tag="ACH3L1_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFT4077302.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="ACH3L1_002610"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="ACH3L1_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="ACH3L1_002611"
     CDS             6713..7195
                     /locus_tag="ACH3L1_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFT4077303.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="ACH3L1_002612"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="ACH3L1_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFT4077304.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="ACH3L1_002613"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="ACH3L1_002613"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFT4077305.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="ACH3L1_002614"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="ACH3L1_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFT4077306.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="ACH3L1_002615"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="ACH3L1_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFT4077307.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="ACH3L1_002616"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="ACH3L1_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFT4077308.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="ACH3L1_002617"
     CDS             10939..11730
                     /locus_tag="ACH3L1_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFT4077309.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="ACH3L1_002618"
     CDS             11751..13037
                     /locus_tag="ACH3L1_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFT4077310.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="ACH3L1_002619"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="ACH3L1_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFT4077311.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="ACH3L1_002620"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="ACH3L1_002620"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFT4077312.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="ACH3L1_002621"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="ACH3L1_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFT4077313.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="ACH3L1_002622"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="ACH3L1_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFT4077314.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="ACH3L1_002623"
     CDS             complement(16985..17275)
                     /locus_tag="ACH3L1_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFT4077315.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="ACH3L1_002624"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="ACH3L1_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFT4077316.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="ACH3L1_002625"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="ACH3L1_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFT4077317.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="ACH3L1_002626"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="ACH3L1_002626"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="ACH3L1_002627"
     CDS             19137..20378
                     /locus_tag="ACH3L1_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFT4077318.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5301 a         5357 c         4814 g         5049 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attgcaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccaa gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgttgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacaa cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtatttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAZXUL010000072        20331 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZXUL010000072 DAZXUL010000000
VERSION     DAZXUL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20331
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.072"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..122
                     /locus_tag="ACH3L1_002628"
     CDS             <1..122
                     /locus_tag="ACH3L1_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFT4077319.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="ACH3L1_002629"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="ACH3L1_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFT4077320.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="ACH3L1_002630"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="ACH3L1_002630"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFT4077321.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="ACH3L1_002631"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="ACH3L1_002631"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFT4077322.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="ACH3L1_002632"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="ACH3L1_002632"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFT4077323.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="ACH3L1_002633"
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="ACH3L1_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFT4077324.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="ACH3L1_002634"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="ACH3L1_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFT4077325.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6135..7505
                     /gene="cpsG"
                     /locus_tag="ACH3L1_002635"
                     /gene_synonym="manB"
     CDS             6135..7505
                     /gene="cpsG"
                     /locus_tag="ACH3L1_002635"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFT4077326.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLTE"
     gene            7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACH3L1_002636"
     CDS             7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACH3L1_002636"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFT4077327.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8956..10434
                     /gene="wzxC"
                     /locus_tag="ACH3L1_002637"
     CDS             8956..10434
                     /gene="wzxC"
                     /locus_tag="ACH3L1_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFT4077328.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10614..11894
                     /gene="wcaK"
                     /locus_tag="ACH3L1_002638"
     CDS             10614..11894
                     /gene="wcaK"
                     /locus_tag="ACH3L1_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFT4077329.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11891..13111
                     /gene="wcaL"
                     /locus_tag="ACH3L1_002639"
     CDS             11891..13111
                     /gene="wcaL"
                     /locus_tag="ACH3L1_002639"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFT4077330.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13122..14516
                     /gene="wcaM"
                     /locus_tag="ACH3L1_002640"
     CDS             13122..14516
                     /gene="wcaM"
                     /locus_tag="ACH3L1_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFT4077331.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14691..15584
                     /gene="galF"
                     /locus_tag="ACH3L1_002641"
     CDS             14691..15584
                     /gene="galF"
                     /locus_tag="ACH3L1_002641"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFT4077332.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15957..17042
                     /gene="rfbB"
                     /locus_tag="ACH3L1_002642"
     CDS             15957..17042
                     /gene="rfbB"
                     /locus_tag="ACH3L1_002642"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFT4077333.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            17042..17941
                     /gene="rfbD"
                     /locus_tag="ACH3L1_002643"
     CDS             17042..17941
                     /gene="rfbD"
                     /locus_tag="ACH3L1_002643"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFT4077334.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            18000..18878
                     /gene="rfbA"
                     /locus_tag="ACH3L1_002644"
     CDS             18000..18878
                     /gene="rfbA"
                     /locus_tag="ACH3L1_002644"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFT4077335.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            18883..19428
                     /gene="rfbC"
                     /locus_tag="ACH3L1_002645"
     CDS             18883..19428
                     /gene="rfbC"
                     /locus_tag="ACH3L1_002645"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFT4077336.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            19444..>20331
                     /gene="wzx"
                     /locus_tag="ACH3L1_002646"
     CDS             19444..>20331
                     /gene="wzx"
                     /locus_tag="ACH3L1_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFT4077337.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSL"
BASE COUNT         5100 a         4824 c         5470 g         4937 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctag gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gcactgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat acccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgagc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgatttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacctgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgtgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacctacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tggtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt
     6001 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
     6061 cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt cagggccaac tattgcctga
     6121 aaaagggtaa cgacatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
     6181 taggcgaaga actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc
     6241 tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct caccagcgaa accttaaaac
     6301 tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt gctggatatc ggtatgtccg
     6361 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
     6421 ctgccagcca taatccgatg gattacaacg gcatgaagct ggtgcgcgag ggggctcgca
     6481 cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct ggctgaagcc aacgactttc
     6541 ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat caacctgcgt gacgcttacg
     6601 ttgatcacct gttcggttat atcaatgtca aaaatctcac gccgctcaag ctggtgatca
     6661 actccgggaa tggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagccc
     6721 tcggcgcgcc cgtggaatta atcaaagtac acaacacacc ggacggcaat ttccccaacg
     6781 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac
     6841 acggcgcgga tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg
     6901 aaaaagggca gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg
     6961 aaaaaaatcc cggcgcgaag atcatccatg atccgcgtct ctcttggaac actattgatg
     7021 tggtgactgc tgcaggcggc acgccggtaa tgtcgaaaac cggacacgcc tttattaaag
     7081 aacggatgcg caaggaagac gccatctacg gtggcgaaat gagcgcccac cattacttcc
     7141 gtgatttcgc ttactgcgac agcggcatga tcccgtggct gctggtcgcc gaactggtgt
     7201 gcctgaaagg aaaaacgctg ggcgaactgg tgcgcgaccg gatggcggcg tttccggcaa
     7261 gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc gattaaccgc gtggaacagc
     7321 attttagccg tgaggcgctg gcggtggatc gcagcgatgg catcagcatg acctttgccg
     7381 actggcgctt taacctgcgc acctccaata ccgaaccggt ggtgcgcctg aatgtggaat
     7441 cgcgcggtga tgtgccgctg atggaagcgc gaacgcgaac tctgctgacg ttgctgaccg
     7501 agtaatgtcg gatcttccct taccccactg cgggtaaggg gctaataaca ggaacaacga
     7561 tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc
     7621 aacgcttttc agatatcacc atcatgtttg ccgggctatg gctggtttgc gaagtcagcg
     7681 gactgtcatt cctctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga
     7741 tgctgggcgg catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat
     7801 ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt cagcgccgga ctggtggcgt
     7861 tcaacaatga tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagca
     7921 tcggactggt ggtttgccgt tcatgtattc gcattggggc gggctggctg cgtaatcatg
     7981 gctacaacaa gcggatggtc gccgtagcgg gggatttagc cgccggacaa atgctgatgg
     8041 agagtttccg taatcagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga
     8101 aaccgggcgg cgtttctaac gactgggcgg gtaacctgca acagctggtc gaggatgcaa
     8161 aagcgggcaa gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcacgagtga
     8221 aaaaactggt ccatcaactg gcggatacca cctgttcggt gctgctgatc cccgacgtct
     8281 ttaccttcaa cattctccat tcacgtctcg aagagatgaa cggcgttccg gtggtgccgc
     8341 tgtatgacac gccgctttcg ggggttaacc gcctgctaaa acgtgcggaa gacattgtgc
     8401 tggcgacgct tattctgctg ctgatctccc cggtgctgtg ctgtattgcg ctggcggtga
     8461 aactcagttc accagggccg gttattttcc gccagactcg ctatggtatg gatggcaagc
     8521 cgatcaaagt gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgaccc
     8581 aggcgacgca gaacgatccg cgcgtcacta aagtggggaa ctttctgcgc cgcacctcgc
     8641 tggacgaatt gccgcagttt atcaatgtgc tgaccggggg gatgtcgatt gtcggtccac
     8701 gtccgcacgc agtagcgcat aacgaacagt atcgccagct cattgaaggc tacatgctgc
     8761 gccataaagt gaaaccgggc attaccggct gggcgcagat taatggctgg cgcggcgaaa
     8821 ccgacacgct ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat
     8881 ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt attcaagggc ttcgttaaca
     8941 aagcggcata ttgatatgag cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggct
     9001 attgccacgg tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt
     9061 atcgacaacc accagttcgg cctgctgact gtgtcgctgg tgattatcgc gctggcagat
     9121 acgctttctg acttcggtat cgctaactcg attattcagc gaaaagaaat cagtcacctt
     9181 gaactcacca cgctgtactg gctgaacgtc gggctggggc ttgtggtatg tgtggcggtg
     9241 tttttgttga gtgatgtcat cggcgacgtg ctgaataacc cggacctggc accgttgatt
     9301 aaaacattgt cgctggcgtt tgtggtgatc ccccacgggc aacagttccg cgcgttgatg
     9361 cagaaggagc tggagttcaa caaaatcggc atgatcgaaa ccagcgcggt gctggcgggc
     9421 ttcactttta cggtagttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt
     9481 tatctggtca atagtgcggt gagaacactg ctgtttggct actttggacg caaaatttat
     9541 tgccccggtc tgcacttctc gctggcgtct gtggcaccga acttacgttt tggtgcctgg
     9601 ctgacggcgg acagcatcat caactatctc aacaccaacc tttcaacgct ggtgctggcg
     9661 cgtattcttg gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgtcgta
     9721 ccgccgatga agctgaaccc aatcatcacc cgcgtgttgt ttccggcgtt tgccaaaatt
     9781 caggacgaca ccgaaaagct gcgtgttaac ttctacaagc tgctgtcgat agtggggatt
     9841 atcaactttc cggcgctgct ggggctgatg gtggtgtcga ataacttcgt accgctggta
     9901 ttcggcgaaa agtggaacag cattattccg gtgctgcaat tgctgtgtat tgtgggtctg
     9961 ctgcgctccg tgggtaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc
    10021 agctttaaat tcaacgtatt caaaacattt ctgtttattc cggcgattgt tattggtggg
    10081 cagatggcgg gcgcaatcgg cgtcacgctc ggtttcctgc tggtgcagat tatcaacacc
    10141 attctgagtt acttcgtgat gattaaaccg gttcttggtt ccagttatcg ccagtacatc
    10201 ctgagtttgt ggctgccgtt ttatctctcg ttgccgacgc tggtggtcag ttatgcgctg
    10261 ggcattgtgc tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccgcg
    10321 ggcgtgctgg cgtttgtggt gatgattgtg ctgtcacgcc atccgctggt ggtggaagtg
    10381 aagcgtcagt tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgaattgct
    10441 gttttcggtt ccgacaacgg ttgccggatg cggcgtaaaa cgccttatcc gtcctacggt
    10501 tctgtgctcg ggacctgtag gcctgataag acgcgccagc gtcgcatcag gcaaaaaacg
    10561 gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    10621 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    10681 tacttgatgc catcaacatc ctcaatccac ataccgaagt ggacgtgatg agccgctatc
    10741 cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg ttcctgcaga
    10801 tgacacaaca caatagcgcg gcgggcgttg tcgggcgcat taaaaaagtc ctccgtcgcc
    10861 gctaccagca tcaggtattg ctctcacgcg tcaccgacac tggcaagctg cgcaatatcg
    10921 ccatcgccca gggattcacc gacttcgtgc gcctgctgtc aggttacgac gccattattc
    10981 aggtcggcgg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcgcttt
    11041 gcacatttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggc cccttccagg
    11101 atgagcaatt taaccaactg gcgaactatg tctttggtca ctgcgacgcg ctgatcctgc
    11161 gcgaatcggt cagccttgat ttgatgaaac gcagaaatat caccaccgca aaagtggaac
    11221 atggcgtcga taccgcgtgg ctggtcgatc accacaccga agacttcacc gccagctatg
    11281 ccgttcaaca ctggctggac gttgccgcac aacagaaaac agtggcaatt accctgcgcg
    11341 aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa aaagcctttg
    11401 ccggggtggt caatcgcatt ctcgatgaag gctatcaggt gattgcgctc tccacctgta
    11461 cgggtatcga cagctataac aaagacgacc gcatggtggc gctcaatctg cgccagcaca
    11521 tcagcgatcc tgcccgttac cacgtagtga tggatgaact caacgatctg gaaatgggca
    11581 aaattctcgg agcctgtgaa ctcaccgtcg gtacgcgcct gcactccgcc attatctcaa
    11641 tgaactttgc tactccggcg attgccatca actatgaaca taaatccgcc gggattatgc
    11701 agcagctggg actgccagag atggcgattg atattcgtca tttactggac ggcagcctgc
    11761 aagcgatggt tgcggatacc ttaggccagc ttccggtgct gaacgctcga tttaacgaag
    11821 ccgtcagtcg cgaacgtcag acgggaatgc agatggtgca gtctgtgctt gagcgcatcg
    11881 gggaggtgaa atgaaggtcg gcttcttctt attgaaattc ccgctgtcgt cggaaacctt
    11941 cgtcctcaat caaattaccg cgtttattga tatgggcttt gaagtggaga ttgtcgcttt
    12001 gcaaaaaggc gatacagaga acacccacgc ggcatggacg aaatacaacc ttgtcgccag
    12061 aacccgctgg ttacaggacg aacctgcggg caaagtagcg aaactgcgcc accgcgccag
    12121 ccagacctta cgtggcattc atcgtaaaaa tacctggcat gcgctcaacc tcaaacgcta
    12181 tggtgccgag tcgcggaacc tgattttgtc tgccatttgt ggtcaggtcg caacaccgtt
    12241 tcgcgccgat gtgttcatcg ctcattttgg cccggcgggg gtaaccgcag caaaactacg
    12301 cgaactgggt atcattcgcg gcaaaattgc caccatcttc cacggtattg atatctccag
    12361 tcgggaagtg ctcaaccact acactcccga atatcaacaa ctgtttcgcc gtggcgacct
    12421 gatgttaccg ataagcgatc tgtgggccgg aaggctgcaa aaaatgggct gccccagaga
    12481 aaaaatcgcc gtatcgcgta tgggcgtgga catgacgcgc tttagcccgc gtccggtgaa
    12541 agcgcccgca acgccgctgg aaatcatatc tgtcgcacgc ttaaccgaga aaaaaggcct
    12601 gcatgtggcg attgaagcct gccgtcagtt gaaagagcag ggcgtggcat ttcgctatcg
    12661 catcctcggc attggcctgt gggaacgacg cctgcgcacc atcatcgaac aatatcaact
    12721 ggaagatgtg gtagagatgc cgggctttaa accgagccat gaagtgaagg agatgctcga
    12781 cgacgcggat gtcttcctgt tgccatcggt aacgggcgcg gatggcgata tggaaggcat
    12841 tccggtggcg ctaatggaag cgatggcggt cggtattccg gtggtttcta ctctgcatag
    12901 cggaattcct gaactggtgg aggccgataa atccggctgg ctgctgcctg agaacgatgc
    12961 tcgcgcactg gcacaacgtc tggcggcgtt tagccaactg gataccgacg aactggctcc
    13021 ggtcgtcaaa cgtgcgtgcg aaaaagtcga acacgatttt aaccagcagg tgattaatcg
    13081 agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt aaaacactct
    13141 cccgacgcac cttcctgacg gcaagctcgg cgcttgcctt cctccatacc cctttcgctc
    13201 gcgcacttcc cgcccgacaa agcgttaaca ttaacgacta caacccacat gactggatcg
    13261 cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc ggattcgttt
    13321 gtgacaatat caacaccggc atcttcatcc ctcccggcaa aacgttacac atccttggaa
    13381 gcctgcgcgg caacggcaga gggcgatttg tcttacagga cggcagccag gtgacagggg
    13441 gagagggcgg cggtatgcat aacattaccc tggatgtgcg tggttctgac tgcaccatca
    13501 aagggctggc gatgagcggc tttggcccgg taatgcagat ttatattggc ggcaaaaaca
    13561 aacgggtcat gcgcaacctg accatcgata acctcactgt cagccacgct aattacgcca
    13621 tcttacgcca gggatttcat aaccagatta tcggtgccaa cattaccaac tgtaagttca
    13681 gcgacttaca gggcgacgcc attgaatgga acgtggcaat taacgacagt gatattttga
    13741 tatctgacca tgtcatcgag cgtatcaact gtaccaacgg caaaatcaac tggggcatcg
    13801 gcataggcct tgcgggaagc acttatgata acaactaccc ggaagaccag gcagtgaaaa
    13861 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat gttgaaaatg
    13921 gtaaacattt tgttattcgt aatatcaatg cccgcaatat cacgccggat ttcagtaaga
    13981 aagcgggcat tgataacgcg acagtcgcta tttacggttg tgacaatttc gtgattgata
    14041 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaaat
    14101 atctctcgat accgcaaaat ttccgagtga ataatattca actggataat acccatcttg
    14161 cttataaatt gcgcggcatc caaatctccg ccgggaatgc cgtctccttt gtgtcgctaa
    14221 ctaacattga gatgaagcgt gcctcgctgg agttacacaa caagccgcaa catcttttta
    14281 tgcgaaatat caatgtgatg caggaatcct cagttggacc agcattgagc atgaacttcg
    14341 acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg ctgtctcttg
    14401 caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac aggattaatc
    14461 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg gagtagattt
    14521 gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca atccacaacg
    14581 aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat gctggcgaaa
    14641 aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata atgacgaatt
    14701 taaaagcagt tattcctgta gcgggtctcg ggatgcatat gttgcctgcc actaaggcga
    14761 tacccaaaga gatgctacca atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    14821 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
    14881 aaaaccactt cgacacctct tatgagttag aatcactcct tgagcagcgc gtgaagcgtc
    14941 aactgctggc ggaagtacag tccatctgtc cgccgggcgt gatcattatg aacgtgcgtc
    15001 agggcgaacc tttaggttta ggccactcca ttttgtgtgc gcgacctgcc attggtgaca
    15061 acccatttgt cgtggtactg ccagacgttg tgatcgacga tgccagcgcc gacccgctac
    15121 gttacaacct tgctgccatg attgcacgtt tcaacgaaac gggccgcagc caggtgctgg
    15181 caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagactaaa gagccgctgg
    15241 accgtgaggg taaagtcagc cgcattgttg aatttatcga aaaaccggat cagccgcaga
    15301 cgctggactc agacatcatg gccgtaggtc gctatgtgct ttctgccgat atttggccgg
    15361 aactggaacg tactcagcct ggtgcatggg gacgtattca gctgactgat gctattgccg
    15421 agctggcgaa aaaacaatcc gttgatgcaa tgctgatgac cggcgacagt tacgactgcg
    15481 gcaaaaaaat gggctatatg caggcgtttg tgaagtatgg cctacgcaac ctgaaagaag
    15541 gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    15601 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcataa gttttgctca
    15661 ttgaattttc ctgccgttgc cgtttttata aagcctataa aaacaagaag ttacatagct
    15721 catattattc aaatttttcc aggattttcc ttgtttctac ggctgtttgg taagacaatt
    15781 aacgtttgaa tttttcggat ttggcgcgag tggctaacgc tcgccacatc gttgagatgc
    15841 atgcagtgca ctggtagctg ttaagccagg ggcggtagcg tgtattaata cctctattaa
    15901 tcaaatctgg agcagtctat ttcacagcat gctctatagc tatatggaat aaaaaagtga
    15961 agatacttgt tactggtggc gcagggttta ttggttctgc tgttgttcgt cacataataa
    16021 ataatacgca agatagtgtt gttaatgtcg ataaattaac atacgccggg aacctggaat
    16081 cactcgctga tgtttctgat tctgaacgtt atgcttttga gcatgcagat atctgcgatg
    16141 ctgtagccat gtcgcgtatt ttcgcacagc accagccaga cgcggtgatg cacctggcgg
    16201 cagagagcca cgttgaccgt tcaataacgg gccctgcggc atttattgaa accaatattg
    16261 tggggactta tgttctttta gaagcggcgc gcaattactg gtctgctctg aatgatgaaa
    16321 agaaaaaaag tttccgcttt catcatattt ctactgatga agtttatggt gacttacccc
    16381 atccggatga agcaaataat aacgaagcgt taccgctatt tacggaaacg acagcttacg
    16441 cgccaagtag cccgtattct gcgtctaaag cttccagcga tcatttggta cgtgcctgga
    16501 agcggactta tggtttacca accattgtga ctaattgctc gaacaattat ggtccttatc
    16561 acttcccgga aaaacttatt ccattggtta ttctgaatgc tctggaaggt aaggcattac
    16621 ctatttatgg taaaggggat caaattcgcg actggttgta tgtagaggat catgctcgtg
    16681 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacata
    16741 acgaaaagaa aaacatcgac gtagtgctca ctatttgtga tttgttggat gagattgtac
    16801 cgaaagagaa atcttatcgt gagcaaatta cttatgttgc cgatcgcccg gggcatgacc
    16861 gccgttacgc cattgatgcc gataaaatta gccgcgaatt gggctggaaa ccgcaagaaa
    16921 cgtttgagag cgggattcgt aaaacggtgg aatggtatct ggctaataca aattgggttg
    16981 agaatgtgaa aagcggtact tatcagtcgt ggattgaaca aaactatgag ggccgtcagt
    17041 aatgaatatc ctgcttttcg gcaaaacagg gcaggtgggt tgggaactgc agcgtgctct
    17101 ggctcctctg ggtaacttga ttgcgctcga tgttcactcc actgattatt gtggagattt
    17161 cagcaacccc gaaggtgtgg ctgaaaccgt caaaaaaatt cgccccgatg ttattgttaa
    17221 tgctgctgct cacactgcag tagacaaagc ggaatcagaa ccgaatttcg cgcaattact
    17281 taacgcgaca tgcgtcgaag cgattgcaaa agcagctaat gaagtcggag cctgggttat
    17341 acactactcc actgattatg ttttcccagg caacggtgac acgccatggc tggaaacgga
    17401 tgcaacagca ccgctaaatg tctacggtga aaccaagcta gctggggaaa aagcgttaca
    17461 agaacattgc gcaaagcatc ttattttccg taccagctgg gtatacgctg gtaaaggaaa
    17521 taactttgcc aaaacgatgt tgcgcctggc aaaagaacgc gaagagttgg ctgtaataaa
    17581 cgatcaattc ggcgcaccaa cgggggctga attactggct gattgcaccg ctcatgccat
    17641 tcgcgtggcc gcaaataaac cagaagttgc tggtttgtac catctggttg ctggtggtac
    17701 aacaacctgg cacgattatg ccgctctggt atttgaagaa gcgcgcagag cggggatcaa
    17761 ccttgctctt aacaaactta atgccgtgcc gacaacggcc tatcccacac cagcacgtcg
    17821 tccccataat tctcgcctca ataccgaaaa atttcagcag aactttgcgc ttgtcttgcc
    17881 tgactggcag gtgggcgtga aacgtatgct caacgaatta tttacgacca cggcaattta
    17941 acaaattatt gcatctcgct catgatgcta gagcgagatg aattaaaagg aatggtgaaa
    18001 tgaaaacgcg taaaggtatt attctggctg gtggttccgg cactcgtctt tatcctgtga
    18061 caatggctgt gagcaaacaa ctgctaccga tttatgataa accgatgatt tattatccgc
    18121 tttcaacgct tatgttggcg ggtattcgag atattctgat tatcagtacg ccgcaggata
    18181 caccacgttt tcaacaattg cttggtgatg gaagccagtg ggggcttaat ctgcaatata
    18241 aagtacaacc gagtccggat ggtctggcgc aagcatttat cattggtgaa gaatttatag
    18301 gtggtgatga ttgtgcgctg gtacttggtg ataatatctt ctatggtcac gacctgccga
    18361 aattaatgga taccgctgtt aacagagaga gtggcgcaac ggtttttgcc tatcacgtaa
    18421 atgatcctga gcgctacggt gtggtggaat ttgatgataa cggtacggca attagtctgg
    18481 aagaaaaacc gcaggaacca aaaagtaact atgcggttac tgggctttat ttctatgaca
    18541 atgacgttgt agaaatggcg aaaaacctta aaccatctgc acgtggcgaa ctggaaatta
    18601 ccgatattaa ccgtatttat atggaccaag gacgtttgtc tgtcgctatg atggggcgtg
    18661 gttatgcatg gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg
    18721 caacaattga agagcgtcag gggctgaaag tttcctgccc ggaagaaatt gctcaccgta
    18781 aaggctttat tgatgctgag caagtgaagg tattagccga gccgctgaag aaaaatgctt
    18841 atggtcagta tctgctgaaa atgattaaag gttattaata aaatgaacgt aattaaaact
    18901 gaaattcctg atgtgctaat ttttgaacca aaagtatttg gtgatgagcg gggctttttt
    18961 atggaaagct tcaatcagaa agttttcgaa gaggctgtag gacgaaaggt tgagtttgtt
    19021 caggataacc attctaaatc aactaagggt gtgttacgcg gactgcacta tcagctggaa
    19081 ccttatgctc aaggaaaact ggtacgctgt gttgtcggtg aagtttttga cgtagctgtt
    19141 gatattcgga aatcatcacc aacatttggc aagtgggttg gtgtaaattt gtcggcggag
    19201 aataagcgtc aattatggat accggaagga ttcgctcatg ggttttgcgt tcttagtgat
    19261 gaagcagaat ttgtctacaa aacaaataat ttctattcaa aaatgcaaga gcgtgggatt
    19321 ttatggagcg acaaaagtat aaatatagaa tggccggttc aaaatccatt gctttctgat
    19381 aaagatatta atggtcaaaa atttgtagat gctgattatt ttatatgata aagaaatgta
    19441 ataatgagca taataaaaaa tagtgtctgg aacctttttg gctatgcaat accaacttta
    19501 attgctatcc cctcgctagg atttctcgct cgaggtttgg ggcctgaagg tttcggtgtt
    19561 tatacaattg caattgcact tgttgggtat gctggaattt ttgatgtagg cctgactcgc
    19621 tctgttatta gagaaattgc aattcatcgt gataatcatc atgaaagaac caaggtaatt
    19681 tcaacaagta catctttttt ggtgctattt tcatgctttg gtgctttttt attattgatt
    19741 ttctctgatg gaattgttaa ttatctaaaa atttctggtg ttgaacatag tgatatacaa
    19801 ctagcattta aactgttggc tatttgcatt ccattattta ttctaaatca attatggtca
    19861 gccattcttg agggggatga aaaatttggc attgtaaata ttcaaaaatc tatatcaagc
    19921 tcttgcatcg cgggaattcc ggccatattt gttttttata gtgctacatt gtcggcagcg
    19981 gttgctggtt taatatttgc aagagttatt tcgattttag tctctgccta ttatgtcagg
    20041 aatgatatta aaatttcggg ggttcatttt tgttataaaa cctttaaacg actctttttc
    20101 tttggcggtt ggatgacagt aagtaatatc ataagtccgg tcatggtgta ttttgatcga
    20161 tttatagtgt caaatattat gggggcagat aaagttgcat tttattctgc accagcggag
    20221 gttattctaa aattaggaat aatacctgca gcaatcggga gggcagtgtt tccaaggtta
    20281 agtaacatca aagactttaa agaatttaaa cgtaatgtaa ataaatcatt g
//
LOCUS       DAZXUL010000073        20291 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZXUL010000073 DAZXUL010000000
VERSION     DAZXUL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20291
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.073"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="ACH3L1_002647"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="ACH3L1_002647"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFT4077338.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="ACH3L1_002648"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="ACH3L1_002648"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFT4077339.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3001)
                     /locus_tag="ACH3L1_002649"
                     /pseudo
     CDS             complement(1729..3001)
                     /locus_tag="ACH3L1_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3184..4389)
                     /locus_tag="ACH3L1_002650"
     CDS             complement(3184..4389)
                     /locus_tag="ACH3L1_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4077340.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4489..4845)
                     /locus_tag="ACH3L1_002651"
     CDS             complement(4489..4845)
                     /locus_tag="ACH3L1_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFT4077341.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4981..5088)
                     /gene="rprA"
                     /locus_tag="ACH3L1_002652"
     ncRNA           complement(4981..5088)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACH3L1_002652"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5274..6386)
                     /gene="ydiK"
                     /locus_tag="ACH3L1_002653"
     CDS             complement(5274..6386)
                     /gene="ydiK"
                     /locus_tag="ACH3L1_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFT4077342.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6775..9831
                     /locus_tag="ACH3L1_002654"
     CDS             6775..9831
                     /locus_tag="ACH3L1_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFT4077343.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9828..10238
                     /gene="menI"
                     /locus_tag="ACH3L1_002655"
     CDS             9828..10238
                     /gene="menI"
                     /locus_tag="ACH3L1_002655"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFT4077344.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10338..10526
                     /locus_tag="ACH3L1_002656"
     CDS             10338..10526
                     /locus_tag="ACH3L1_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFT4077345.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11074..11442
                     /gene="sufA"
                     /locus_tag="ACH3L1_002657"
     CDS             11074..11442
                     /gene="sufA"
                     /locus_tag="ACH3L1_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFT4077346.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11451..12938
                     /gene="sufB"
                     /locus_tag="ACH3L1_002658"
     CDS             11451..12938
                     /gene="sufB"
                     /locus_tag="ACH3L1_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFT4077347.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12948..13694
                     /gene="sufC"
                     /locus_tag="ACH3L1_002659"
     CDS             12948..13694
                     /gene="sufC"
                     /locus_tag="ACH3L1_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFT4077348.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13669..14940
                     /gene="sufD"
                     /locus_tag="ACH3L1_002660"
     CDS             13669..14940
                     /gene="sufD"
                     /locus_tag="ACH3L1_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFT4077349.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14937..16157
                     /gene="sufS"
                     /locus_tag="ACH3L1_002661"
     CDS             14937..16157
                     /gene="sufS"
                     /locus_tag="ACH3L1_002661"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFT4077350.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16170..16586
                     /gene="sufE"
                     /locus_tag="ACH3L1_002662"
     CDS             16170..16586
                     /gene="sufE"
                     /locus_tag="ACH3L1_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFT4077351.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16735..17739
                     /gene="ldtE"
                     /locus_tag="ACH3L1_002663"
     CDS             16735..17739
                     /gene="ldtE"
                     /locus_tag="ACH3L1_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFT4077352.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17802..18038)
                     /gene="lpp"
                     /locus_tag="ACH3L1_002664"
     CDS             complement(17802..18038)
                     /gene="lpp"
                     /locus_tag="ACH3L1_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFT4077353.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18349..19761)
                     /gene="pykF"
                     /locus_tag="ACH3L1_002665"
     CDS             complement(18349..19761)
                     /gene="pykF"
                     /locus_tag="ACH3L1_002665"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFT4077354.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(19769..19963)
                     /locus_tag="ACH3L1_002666"
     CDS             complement(19769..19963)
                     /locus_tag="ACH3L1_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077355.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT         5319 a         4862 c         5177 g         4933 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccaacca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcctgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtatccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaaagg ttttgctgtt catttgcggt aattcattag
     2401 ctacgctggc gtccaccaac tggctgggga atttgctttt caacagcatc agcgtgatca
     2461 gtacaaacag aatacccgga ataatcagcc catagccgta ccagatatta ttgagcaaca
     2521 tatagctcac cagcattggg tagaacattt gcccaaatga caccatcgct ttaaccagta
     2581 tgaccgccga accagaggct ttcggaaagc attccatgag cgcggggtag ccacccgtat
     2641 ccagcgctga gttagcgata cctacgcaca ctgccagacc gtaggcgaga gttaaattcg
     2701 ggcaagcggg aataccaaag aagaatagca gatacattat tactgccatt aatatcaccg
     2761 cccgacgacc aaacttatcg gagatcacac cgaagaataa aatactgatc aatcgcccca
     2821 aaccgatacc ggaaattaaa taggcaatgc ccgcgttgtc agtggaaaac ttttctgaca
     2881 gagatgtcat attttgggca agcgtaataa cactaatgcc gtgcaggaaa tagctgaagt
     2941 aaatacaaag aacagccagg ataaatggcg tgctgaaaga taatttcgtt accgtacgta
     3001 ttgttctccg acggtttaca taattactat actgaagatg attttccctg ggtacatggc
     3061 ataaagttta tgtttccagg aatattgcat gtcatagttt gtttttgatc gcgattttcc
     3121 ttagtaagcg cagaaagagt tatttatccg taagatcttg catgacctga aaagaatatt
     3181 gcattaccca cccggagcga cattttcctt tagcgagtga tgccgcgttt ttttgcggct
     3241 gcgtaagccg ataaacagtg ccagtaaaaa accgatggca gtatcgaacc acataatatc
     3301 ggcaatactt ctttgggaca gatgagccgt aatcaacgga atagtaaagg tcgcaatact
     3361 gcccgtactg taatagatcc ctgtagcttt acctttagcg taagggaaac gttcagccat
     3421 taacgtcagg ccaatttgca caacacctcc agcagaggta aaaccaatga caaaagcaaa
     3481 tattatcacc acataaaatg tgggatgcag gcagacggta aacagagcaa taaatgagat
     3541 aaaggtgtac agcatcagta atgttgttgg gcgaacggta ttacgaatga gtggagcggt
     3601 aataaataca caaagcagcg aacccacggt gtagatactg agtagtttga tcgacatagt
     3661 gtatgacatg cctgcaacaa attgtccgta ctgtgccagc cactggctaa ccagataaaa
     3721 cgttgccatt gagatatagc catataaggt ataactggct aaatcgataa ttgaacagcg
     3781 atgttccgta gagctggtgg ttttctttat gacaggtaag cgacgacccg gatggggtgg
     3841 gaacgtacaa cggtataaaa acagagcgtt aataaacata atgcctgcag caatcataaa
     3901 ggaccaaccg aaccacagtt cagcccacac taacaggcta atgattagcg gtaataaaaa
     3961 ttgtccgctg gaaacaaatg ctttaattaa aatattggct gtcccaggtg agcgtggaaa
     4021 agcttccatc aaactgggat aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag
     4081 aaagccaaaa acataagcga taatgatgtt attggtctgc aggatgtcaa aaaagaaggc
     4141 catatagcag cacatcccga gcatgataaa agggcggcga ccaaagcgat cggataataa
     4201 tcctgcaaaa agcaagacac ttaatcgacc aatgcccagc gatgagataa ctatcgagac
     4261 acccgcggca ttagtctgcc aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat
     4321 gacgcccata ccatgcacca ggtaattaaa atacaaccca agtgcggtag ggtaataggg
     4381 atttttcata ggttctactt agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt
     4441 atccattcat tattgttgta acaacgacaa ttcctttctg tggaaaaatt aacaaagccg
     4501 ctccagatcg tgtgcatata attcagcggc taccgattga tagatcttcc agtcaataaa
     4561 attgccatca gggtagactt gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt
     4621 gttgccgatg cggttattta ttttctctat gatggcatga agatgttttt ttcgttgctg
     4681 acgcatagcc aacagttgct cgactacaca atcaggaatg gcgcatttgc cattttccca
     4741 ctgacgccag ctttcactgt caccggtctg ggcaatccag gtcgcgcatt catcaatagt
     4801 catggcaaaa atatggcgga gtgcctggag ttcataagcg ttcatcgtgt aatggctgca
     4861 tgaaataaac aataaaatat aaataacatt atcagcctgc tgacggcttg aagagagtca
     4921 cagtatcttg tgcaacatta ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg
     4981 ttaaaaaaaa gcccatcgtg ggagatggga aaagactaca cacagcaatt cgttgtttca
     5041 ctcaggggat ttccatgctt ataaatcaat atgttgattt ataaccgtga gctaatagta
     5101 ggcatacgga ctttttgcgt cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat
     5161 ttgcgacagg tactaccagc tgaatggcgg taagcaggaa aatatgggga agagcaagca
     5221 aatatcaatt agcggtgatt aacaatcagg cggcagagcc gcctgatgag aaattactta
     5281 ttcggtttct cgatttcgcc aagttcttcg agaatttctt ccggttggtc cgtcggtggc
     5341 gggacttctt ccacccacgc ggcaaacaga cgccaggaaa cggctaacag aaccggacca
     5401 ataaacagac cgatcatccc gaaagcaatc aaaccaccaa taacgccaga gaggatcagg
     5461 atcagcggta aatcggcacc catgcgaatt aacattgggc ggatgacgtt atccagcgtg
     5521 ccaaccacac cgctccacac taacaatacc gttccccagg tggtatcgcc ggtccagtag
     5581 agccagataa tcgccggaat cagtaccggc aacgggccaa gctggacaag gcaggagagg
     5641 atcattaaca ccgttagcaa cgttgcataa ggtacgccgg atacggcgag gccgataccg
     5701 ccaagcactg cctgtactaa cgccgtcacc accacaccca gcgccaccgc gcggatagcc
     5761 tgtgccgcca gcagcacggc ggcatctccg cgaacgcctg ccagacgggt tgcaaaatgg
     5821 cgaatgcctt gtgccacctg ttcaccgcgc cagtacagca gggcactgaa gagaagcatc
     5881 agcgcacaat gcaccataaa gcgcccgata tgcgccgcct gcccaacgaa ccaggtggtg
     5941 gtggtgccaa tataagggcg gactttcgcc atgatcgccg taccccccat atccagcaag
     6001 ttgtgccagc ctgcatacag cttcgcgcca atcaccggaa tggtattaag ccacgccaga
     6061 tcgggtaacg tcatgtcgcc gctggaaatg gctttaatta gcgggccgct gccgtcgacg
     6121 atactgttaa ccagcaaagc aatagggatg ataaacacca tcactaataa cagcgtcatc
     6181 accagaacgg cgagagagcg gcggccaaac atgatctttt gcaaacgtaa caataccggc
     6241 caggtggcga taaccaccgt accggcccat gcaaagccga gaataaaggg ttgaacaatc
     6301 cacagacatg ccacaatcat gatggctaaa aacagcaccg aaagcagaat ttgtgcgaca
     6361 tccctgggct gacgaacatt taccataact acttttcacc ttttttgtgc gtcaaatcgt
     6421 cgacgcgacg tgaacacgcg aaactcacct gcataatgat gagcaatttc agcggttttt
     6481 aacaggccga ttctgccctt aattctgatg ggcgtgttag aaaaaaatgt ggtacaacaa
     6541 ctcaggcaac acgcaaacga tttactcgtc gtatttcaag ttgcatgtgc tgcggctacg
     6601 ttcgttcacc ccagtcactt actattgtaa gctcctggag ttcactcgct tgccgccttc
     6661 ctgcaactcg aaatccatag agtaaataaa catcaccttt cagtgcgcta ttgcagccgc
     6721 ctgaaagaat agtgtatcga cctacatcac agaccgcagg aaagggtcaa tataatgatt
     6781 ccacagattt ccctggcacc cggcgtcgtt caactggtgc ttaatttttt gcaagagctg
     6841 gagcaacaag gttttaccgg cgatgcggcg acaagttatg ccgatcgtct gacaatgtcg
     6901 accgacaaca gtatttacca acttctcccc gatgcggtgg tatttccgcg ttcaaccgca
     6961 gatgtggcgc tgatcgcccg tcttgccgcg caggaacgct attcatcgct gatctttacc
     7021 ccccgcggcg gcggcaccgg cactaacggt caggcgctca accaggggat tattgttgat
     7081 atgtcccgcc atatgaaccg catcatcgaa attaaccctg aagagggctg ggtgcgcgtt
     7141 gaggccgggg tgataaaaga ccaactcaat cagtacctga aaccgttcgg ctactttttt
     7201 gcgccggaac tttcgaccag caaccgggca acgctcggtg ggatgatcaa taccgatgca
     7261 tccggtcagg gatcgctggt ctatggcaaa acgtcagatc acgtacttgg cgtacgcgcg
     7321 gtgttgttgg ggggcgatat tctcgatacg caacctttac ccgtcgagct ggcggaaacg
     7381 ctgggtaaat ccaataccac aatcgggcga atttataaca cggtttatca acgttgccgt
     7441 cagcaacgcc agttaatcat cgacaacttc cccaaactta accgctttct taccggttac
     7501 gatctgcgtc atgtctttaa cgatgagatg accgagttcg acctgacgcg cattctgacg
     7561 ggttcagaag ggacgctggc ctttattacc gaagcgcggc tggatattac gcgcttgcct
     7621 aaagtgcgcc gtctggtgaa tgtcaaatat gactcttttg actctgcgct gcgtaacgcg
     7681 ccgtttatgg ttgaggcgcg ggcgctttcg gtagagacgg tggactcaaa agtgctgaat
     7741 ctggcgcggg aagatattgt ctggcattcc gtcagcgagt tgattaccga tatgcctgac
     7801 aaagagatgc tcggactgaa cattgtggaa tttgctggtg atgatgaggc gctgattgac
     7861 gagcgagtta atgcactctg tgtgcggctt gatgagctga ttgccagcca acaggcaggt
     7921 gtgattggct ggcaggtgtg ccgcgagctg gcgggggttg aacgtatcta tgcgatgcgc
     7981 aaaaaagccg ttggtctgct tggcaatgcc aaaggtgccg ctaagccaat tccgtttgct
     8041 gaggatacct gcgtaccgcc ggaacatctg gccgattata ttgctgaatt tcgcgcgctg
     8101 ctcgacagcc acggcttaag ctacggtatg ttcggtcacg tcgacgcagg tgtcttgcac
     8161 gtccgtccgg cactggatat gtgcgatccg caacaagaga ttttgatgaa gcaaatctct
     8221 gatgacgtgg tggcgctgac tgcgaaatac ggtggtttgt tgtggggcga gcacggcaaa
     8281 ggttttcgcg ctgaatacag cccggcgttt ttcggtgagg aactttttgc agaactgcgc
     8341 aaagtgaaag cggcatttga cccgcataac cgactcaacc cagggaagat ttgcccgcca
     8401 gaaggtctcg atgcgccgat gatgaaagtg gacgcggtga agcgcggtac attcgatcgg
     8461 cagatcccca ttgcggtacg ccagcagtgg cgcggtgcga tggagtgtaa cggcaacggt
     8521 ttatgcttca actttgatgc ccgtagtccg atgtgtccgt cgatgaagat cacccagaac
     8581 cggattcatt caccgaaagg gcgcgcaacg ctggtgcgtg aatggctgcg cttgttggcg
     8641 gatcgcggcg ttgacccact caaactggaa caagaactgc ctgaatccgg cgtcagtttg
     8701 cggacgttaa ttgcccgcac gcgcaatagc tggcatgcga ataaaggcga atatgacttc
     8761 tcacacgaag tcaaagaggc gatgtcgggc tgtctggcct gtaaagcttg ttcgacccag
     8821 tgccccatca aaattgatgt gccggagttt cgctctcgtt ttctgcagct ctatcacacc
     8881 cgttatttac gcccgcttcg cgaccacctc gttgctacgg tcgagagcta cgcaccgctg
     8941 atggcacgcg cgccgaaaac ctttaacttc ttcattaacc agccgctggt gcgcaaactc
     9001 tcggaaaaac atatcggcat ggttgatttg ccactgctgt cggtcccctc gctacaacaa
     9061 caaatggtgg ggcatcgctc ggcaaacatg acgctggaac agcttgaagc tctcaatgca
     9121 gagcagaaag cgcgcacggt gttggtggtg caggacccct ttaccagcta ttacgatgcg
     9181 caagtggtgg cggattttgt ccgtctggtt gaaaaattag gtttccagcc tgtgttactg
     9241 ccattttcgc caaatggcaa agcccagcat atcaaaggct ttcttaatcg ttttgcgaag
     9301 acggcgaaaa agacggcgga tttcctcaac cgtatggcta agcttgggat gccaatggtg
     9361 ggcgtcgatc cggcgctggt actttgttat cgcgatgaat ataaactggc gctgggcgag
     9421 gaacgtggcg agtttaacgt cttacttgcg aatgaatggc tggcaagcgc acttgagtca
     9481 catccagtgg ctacagtcag cggtgaatca tggtatttct ttggtcactg taccgaggtt
     9541 accgctttgc cgggcgcgcc agcacaatgg gccgcgatat ttgcccgttt tggcgcgaaa
     9601 ctggaaaatg tcagcgtggg ttgctgcggc atggcaggga cttacggaca tgaagcgaaa
     9661 aaccatgaaa attcgctcgg gatctatgag ttatcctggc atcaggctat gcagcgactg
     9721 ccgcgtaacc gctgtctggc gaccggatat tcctgccgta gccaggtaaa acgggttgaa
     9781 ggcacggggg tacgccatcc tgtgcaggct ttactggaga ttattaaatg atatggaaac
     9841 ggaaaatcac cctggaagca ctgaatgcta tgggtgaagg aaacatggtg gggttcctgg
     9901 atattcgctt tgaacatatt ggtgatgaca cccttgaagc gacaatgcca gtagactcgc
     9961 ggacaaagca gcctttcggg ttgctgcatg gaggagcatc cgtggtactg gccgaaagta
    10021 tcggttccgt tgccggttat ttatgtaccg aaggtgagca aaaagtggtt ggtctggaaa
    10081 tcaatgctaa tcacgtccgc tcggcacgag aagggcgggt gcgcggcata tgtaaaccgt
    10141 tgcatctcgg ttcgcgtcac caggtctggc agattgaaat cttcgatgag aaagggcgtt
    10201 tgtgctgttc gtcacgattg acgaccgcca ttttgtgacg ttgtcaccag aaaagtgtga
    10261 cgcatattgc ttttggtcat tatctgcaca gtaaacggtg gtatactgtg caggtcttgt
    10321 ctaaaagcga ggatgctatg tctactcaac ttgatcccac ccagctggcc attgaatttt
    10381 tacgccgtga ccagagcaat ctttcacctg cccaatatct caaacgtctg aaacaactag
    10441 aacttgaatt tgctgacctg cttactctct cttctgctga attaaaagaa gaaatctact
    10501 tcgcctggcg tctgggcgtg cattgatcct gacttaatgc cgcaagttcg ccaaatcgac
    10561 ctggattaac ctccgtccag gttcgatgac cttcttaata ctgcttatct accgcctgat
    10621 attgttcaaa aataatacta atcgcagttt gtgttaaaac ggcgggttag ctttatgaga
    10681 ttattcttat cgccccttca agagctaagc cactgagagt gccggagata agcgccggat
    10741 ggggtagaaa cccttaagcc tgtgtcgcac agacttaagg gttttcttat ttctgaacgc
    10801 tgcacttttt tacctatctc ttaaatagtc attttcaata caactttttc tttttcatca
    10861 cagccttaat gctccttaga taataaccat tatctaacaa tgagatacct aattcttagc
    10921 gtgcctgtta acccacacat caggggctat gcttattaaa agcaggcaaa aagaatgttt
    10981 tacggtaaag cccctgcgtt tgctgggttg aactgataat cattatcact aacatgctgt
    11041 tatacgctga aagcgatgaa gtgaggtaaa tagatggaca tgcattcagg aacctttaac
    11101 ccacaggatt tcgcctggca aggcttaacg ctgacacccg cagcggcgat acacatccgc
    11161 gagctggtgg caaagcagcc aggtatggtc ggcgtgcgct taggtgtgaa gcaaacgggc
    11221 tgcgcgggct ttggctatgt gctcgacagt gttagcgagc cggacaaaga cgatctgctg
    11281 tttgaacacg acggcgcgaa gctgtttgtc ccgctgcaat cgatgccgtt tattgatggc
    11341 acggaagtcg atttcgttcg tgaaggactt aatcagatat tcaaatttca caaccctaaa
    11401 gcccagaatg aatgtggctg tggcgaaagc tttagggtat aggcggtact atgtctcgta
    11461 atactgaagc aactgacgat gtcaaaacct ggaccggcgg cccgctgaac tataaagaag
    11521 gattcttcac ccagttagcc accgatgagc tggcaaaggg gataaacgaa gaggtggtgc
    11581 gcgcaatttc ggcgaagcgt aatgagccgg agtggatgct ggagtttcgt ctaaacgcct
    11641 atcgcgcatg gctggagatg gaagaaccgc actggttgaa agcgcactac gacaagctga
    11701 attatcagga ttacagctac tactcagcac catcgtgcgg taattgtgac gacacttgcg
    11761 cgtctgaacc tggcgcggtg cagcaaactg gcgcgaacgc ctttttaagt aaagaggtgg
    11821 aggcggcgtt tgagcagttg ggcgttcccg tgcgggaagg caaagaggtg gcggtggatg
    11881 ccattttcga ctctgtttcg gttgccacca cttatcgcga aaaactggcg gagcagggaa
    11941 ttattttctg ttcctttggt gaggcgatcc acgatcaccc ggaactggtg cgtaaatatc
    12001 tcggcaccgt ggtgccgggg aatgacaact tctttgcagc acttaatgcg gcggtagcct
    12061 ctgacggtac gtttatttat gtgcctaaag gcgtgcgctg cccgatggaa ctttccacct
    12121 attttcgcat taacgcggaa aaaaccgggc agtttgagcg cacaattctg gtggccgacg
    12181 aagacagcta cgtcagctac attgaaggct gttccgctcc ggtgcgtgac agctatcagt
    12241 tgcacgcggc agtggtggaa gtcatcatcc ataaaaacgc cgaggtgaaa tattccacgg
    12301 tacaaaactg gttccctggc gataacaaca ccggcggtat tctcaacttc gtcaccaagc
    12361 gtgctttgtg cgaaggcgaa aacagcaaaa tgtcatggac gcaatcagaa accgggtcag
    12421 cgattacgtg gaaatatccc agctgcattt tgcgcggcga taactccatt ggtgagtttt
    12481 actcagtggc gctgaccagc ggtcatcagc aagcggatac cggcaccaag atgatccaca
    12541 tcggtaaaaa caccaaatcg accattatct cgaaagggat ctctgccgga catagtcaga
    12601 acagttatcg cggcttagtg aaaatcatgc cgacggcaac caatgcgcgc aatttcactc
    12661 agtgcgactc aatgctgatt ggcgctaatt gtggggcgca taccttcccg tatgtcgaat
    12721 gtcgcaataa cagcgcacaa ctggagcacg aggcaacgac atcacgtatt ggtgaagatc
    12781 aactgtttta ctgcctgcaa cgcgggatca gcgaagaaga cgccatctcg atgattgtta
    12841 acggtttctg caaagacgtg ttctcagaac tgccgctgga atttgccgtt gaagcacaaa
    12901 aactcctcgc catcagtctt gaacacagcg tcggataagg aataaacatg ttaagtatta
    12961 aagatttaca cgtcagcgtg gaagataaag ctatcctgcg tggattaagc ctcgacgttc
    13021 gtcccggcga agttcacgcc attatggggc caaacggttc gggcaaaagt accttatcgg
    13081 caacgcttgc cgggcgcgaa gattatgaag tgacgggcgg cacggttgag ttcaaaggca
    13141 aagatttgct tgcactgtcg ccggaagatc gtgcaggcga aggcatcttt atggccttcc
    13201 agtatccggt ggagattcca ggcgtcagta accagttttt cctgcaaacg gcacttaatg
    13261 cagtgcgcag ctatcgcggc caggaaacgc tcgaccgctt tgattttcag gatttgatgg
    13321 aagagaaaat cgctctcctg aagatgccgg aagatttatt aacccgttcg gtaaacgttg
    13381 gtttttccgg cggcgagaaa aagcgcaacg atattttgca aatggcggtg ctggaaccgg
    13441 agttatgcat tcttgatgag tcggactccg ggctggatat tgacgcatta aaagtggtcg
    13501 ccgatggcgt gaactcgctg cgtgatggca agcgctcatt catcattgtt acgcactacc
    13561 aacgcattct cgactacatc aagcctgatt acgttcatgt gctatatcag ggacgaattg
    13621 tgaaatccgg cgatttcacg ttggtcaaac aactggagga gcagggttat ggctggctta
    13681 ccgaacagca gtaatgcgct gcaacagtgg catcacttgt ttgaagctga agggacaaaa
    13741 cgctcaccgc aagcacagca gcatttacaa caattgctgc gtaccggact gccgacacgt
    13801 aaacatgaaa actggaaata tacgccgctg gaagggctga ccaatagtca gtttgtcagc
    13861 attgcgggag atatatcccc acagcagcgt gatgccttag cgttaacgtt agacgccgtg
    13921 cggctggtgt ttgtcgatgg acgttacgtg cccgcgctga gcgatgcaat tgaaggcagc
    13981 ggatatgaag tgagcattaa cgacgaccgt caggggttac ccgacgctat tcaggcggaa
    14041 gtgtttctgc atttgacgga aagcctggca caaagcgtga cgcatatcgc cgtgaagcgc
    14101 ggtcaacggc cggcaaagcc attgctgtta atgcatatca cccagggcgt ggcaggtgaa
    14161 gaggtgaaca ctgcccatta ccggcatcat ctggatctgg cggaaggtgc cgaagcaacg
    14221 gtgatcgaac attttgtcag ccttaatgat gctcgtcatt ttaccggggc acggttcact
    14281 atcaatgtcg cagcgaacgc ccacttgcag catatcaagc tggcgtttga aaacccggtc
    14341 agtcaccact ttgcccataa cgatttgttg ctggctgacg atgccaccgc atttagccat
    14401 agtttcctgc tgggtggcgc agtgttacga cacaacacca gtacgcaact caatggcgaa
    14461 aacagcacgc tgcggatcaa tagcctggcg atgccggtga aaaacgaggt gtgtgatacc
    14521 cgtacctggc tggaacacaa taaaggtttt tgtaacagcc gacagttgca caaaactatc
    14581 gtcagcgaca aaggccgcgc ggtatttaac ggtttgatca acgtcgcgca gcacgccatc
    14641 aaaacggatg gtcagatgac caacaataat ctgctgatgg gcaaactggc ggaagtggat
    14701 acaaaaccgc agctggaaat ctatgcagat gatgtgaaat gcagccacgg cgcgacggtg
    14761 gggcgtattg atgatgaaca gatgttctat ctgcgctcgc gcgggatcaa tcagcaggat
    14821 gcccagcaga tgatcattta cgcctttgct gccgaactga cggaagcact gcgtgatgag
    14881 gggcttaaac agcaggtgct ggcccgaatc ggtcaacggc tgccaggagg tgcaagatga
    14941 ctttttccgt cgacaaagtg cgggccgact ttccggtgct ttcgcgtgag gtaaacggtt
    15001 tgccgctggc ttatctcgac agcgccgcca gtgcacagaa accgagccag gtgattgacg
    15061 ccgaggccga gttttatcgt catggctacg cggcggtgca tcgcggtatt cataccttaa
    15121 gcgcccaggc gaccgagaaa atggagaacg tgcgcaagcg ggcatcgctg tttattaatg
    15181 cccgttcggc ggaagagctg gtgtttgtcc gcggcacgac ggaagggatc aatctggtcg
    15241 ccaatagctg gggcaacagc aatgtgcggg cgggcgataa catcatcatc agtcagatgg
    15301 agcaccacgc taacattgtc ccctggcaga tgctttgcgc acgcgttggc gcagagctgc
    15361 gtgtgatccc tctcaacccc gacggtacgc tgcaactgga gacgctgcct acgctgtttg
    15421 atgagaaaac tcgcctgctg gcaattactc atgtctccaa cgtgcttggc acagaaaatc
    15481 cactggcgga aatgatcacg cttgcgcacc agcatggcgc aaaagtgctg gtggatggcg
    15541 ctcaggcggt gatgcatcat ccggtggatg ttcaggcgct ggattgcgac ttttacgtgt
    15601 tctccgggca taaactgtat ggccccaccg gaattggcat tctttatgtg aaagaagcct
    15661 tgttgcagga gatgccgccg tgggaagggg gcggttctat gatcgccacc gtcagcctga
    15721 gtgaaggcac tacctggacc aaagcaccat ggcggtttga agccggtaca cccaataccg
    15781 ggggcatcat tggtcttggc gcggcgctgg agtatgtttc ggcgctgggg cttaatagca
    15841 tagccgagta tgaacagaat ctgatgcatt atgcgctatc acagctggaa tctgtaccgg
    15901 atctcactct ctatggccaa caaaacaggc ttggcgttat tgcttttaat ctcggtaaac
    15961 accacgccta tgatgttggc agttttctcg ataattacgg cattgctgtg cgtaccggac
    16021 atcactgcgc tatgccatta atggcctatt acaacgtccc tgcgatgtgt cgggcgtcgc
    16081 tggccatgta taacacccat gaagaagtgg agcgtctggt gaccggcctg caacgtattc
    16141 accgtctgct gggataacag ggaggcacta tggctttatt gccggataaa gaaaaattgc
    16201 tgcgtaattt tttacgctgc gccaactggg aagagaaata tctctacatt attgagctgg
    16261 gccagcgtct gccagaatta cgcgccgaag acggaagccc acaaaatagc attcagggct
    16321 gccagagtca ggtgtggatt gtcatgcgcc agaatgcgca gggaattatt gaattgcagg
    16381 gcgacagcga tgcggcgatt gtgaaagggc ttattgcggt cgtctttatt ctctacgatc
    16441 agatgacgcc gcaggatatt gtcaatttcg atgtgcgtcc gtggtttgaa aaaatggcgc
    16501 tcacccaaca tctcacccca tctcgttcac aaggtctgga agcgatgatt cgcgcaattc
    16561 gcgccaaagc cgctgcactt agctaaacta aagggacagc tttcatccgg ttggctcaca
    16621 ttgccggatg gtcaatgttt acactgaccg gcacaggagt gttttggttt caggtgaaca
    16681 taagcaccat ccgacgtttc agcgtgagtc tccggcaaat acaggaggtt tacaatgaaa
    16741 cgcgcgtctt tgcttacact cacgcttatc ggcgctttta gcgccatcca ggctgcctgg
    16801 gcggttgatt atccgctacc accaaccgga agccgactgg ttgggcaaaa tcaaacgtat
    16861 acggtgcaag aaggggataa aaaccttcag gctattgccc gacgttttga tactgcagca
    16921 atgttgatcc ttgaagccaa taacactatc gccccggtgc caaaacctgg tacgacgata
    16981 actattcctt cacaactgtt attacctgat gcaccgcgtc aggggattat cgttaacctt
    17041 gcagagctgc gcctttatta ttatccgccg ggagaaaata ttgtgcaggt ttatccaata
    17101 ggtattggat tgcaggggct ggaaacgccg gtgatggaaa cgcgtgttgg gcagaaaatc
    17161 cctaacccaa cctggacgcc tacggcaggc attcgtcagc gttcgctgga gcgtggcatt
    17221 aaattaccgc cagtcgttcc tgccggacca aataacccgc taggacgtta cgcactgcgc
    17281 ctcgcgcatg gtaatggcga atacctcatt catggcacca gtgcgccgga cagcgttggt
    17341 ttgcgcgtca gttcagggtg tattcgcatg aatgcaccgg atattaaagc cttgttctcc
    17401 agcgtgcgga cgggaacgcc ggtgaaagtg atcaacgaac cggtgaaata ttccgtggaa
    17461 cctaacggga tgcgttatgt tgaagtacat cgaccactat cagcagaaga acagcaaaac
    17521 gttcagacaa tgccatacac actgccagca ggctttacgc aatttaaaga caataaggct
    17581 gtagatcaga agttagtcga taaagcgttg tatcgtcggg cagggtatcc ggtttcggtg
    17641 agcagtggag caactcccgc agccagcaat gcgccttcag tagagtcagc gcagaatggt
    17701 gaaccagagc aagggaatat gttacgcgcg acgcagtagt ggtaaatagc agacaaaaaa
    17761 atggcgcaca atgtgcgcca tttttcactt cacaggtact attacttgcg gtatttagta
    17821 gccatgttgt ccagacgctg gttagcacga gctgcgtcat ctttagcagc ctgaacgtcg
    17881 gaacgcattg cgttcacgtc gttgctcagc tggtcaactt tagcgttcag agtctgaacg
    17941 tcagaagaca gctgatcgat tttagcgttg ctggagcaac ctgccagcag agtagaaccc
    18001 aggattaccg cgcccagtac cagtttagta gctttcatta ttaataccct ctagattgag
    18061 ttaatctcca tgtagcgtta caagtattac acaaagtttt ttatgttgag aatatttttt
    18121 tgatgggaag gcacttattt ttgatcgttc gctcaaagaa gcatcgaacg ctgggtttta
    18181 gataaaaaaa ttgagagaaa acaggatgct tccatcggat tcatcttaaa taaagtgaga
    18241 ttatatagtg aaatccgatt tgatttttta ttgcttctgg ttatcgatta aataaaaaaa
    18301 gcgcccatca gggcgcttcg atatacaaat taattcacaa aagcaatatt acaggacgtg
    18361 aacagatgcg gtgttagtag tgccgctcgg taccagtgca ccagaaacca taactacgac
    18421 gtcacctttg tgtgccagac cgctctgcag agccagttct ttacccagac ggtagaaatc
    18481 atcagtagaa gtgatctctt taaccagctg cggcacaacg cctttgctca gtaccaactg
    18541 atgagccgtt ttttcgttgg tggtcagtgc caggatggtg gcatccggga agtatttacg
    18601 tactgcgcga gcagatttac cgccctgggt agcaaccacg atcagcggag catccagttt
    18661 ttcagcagtt tcaacggcac cacggcatac cgcttcggtg atgcgcagtt tacggttgtc
    18721 attgttgaac tcgagacggc tgttcatcac gcggtcggta cgttcgcaga tggtcgccat
    18781 gatagaaacc gcttccagcg ggtatttacc ttttgcggat tcaccagaca gcatcactgc
    18841 gtcagtaccg tcgaggatgg cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg
    18901 gtttttgatc atggaatcca gcatctgggt cgcagtgata acgactttac gtgcacggat
    18961 acatttttcg atcatcatct tctgggcgaa gataacttct tctaccggga tttctacacc
    19021 caggtcgcca cgcgcaacca tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt
    19081 gaggccttcc tggttttcga ttttggagat gatgtggatg ttttcgccgc cgtgcgcttt
    19141 caggtgctca cggatttcga taacgtcaga acgcttacga ataaaggaag cagcaacaaa
    19201 gtctacgcct ttttcgcaac caaagatcag gtcctgtttg tctttttcag ccagtgctgg
    19261 cagagcaatg gaaacgccag gcaggttcac acctttgttt tcgcccaggt caccgttgtt
    19321 cagcacttta cagataactt tgttaccttc aatggcggta acttccatac cgatcagacc
    19381 atcgtcaacc agtacggtgt tgccaacaga caggtcagta gtgaaacctt catacgttac
    19441 cgcaaccatt tcgctgttgc cgataacaga tttatcagtg gtgaaagtaa aggtctgacc
    19501 agctttcaga gaaacatcgt taccgccttc cagtttcatg gtgcggattt ccggaccttt
    19561 ggtatcaagc aggatagcgg cggttttacc agttttgctc atcacgttgc gcagattctg
    19621 aatgcgctga ccgtgttctg catagtcacc atgagagaag ttcagacgca taacgttcat
    19681 gccagcgtcc agcattttag ctaacatctc ttcagattcg gttttcggtc cgatggtgca
    19741 aacaattttg gtctttttca tgacagtctt agtctttaag ttgagaagga tgggagaaac
    19801 ttgctttctg ggcgcactgc cggaaagcga atccggtatt acgaaagtgg tggtgcctcg
    19861 ctctaaggat aggtgacatc gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc
    19921 aacgtgagtg tctgtttttg agtcgttgag ttctacagtc caaagtgctg aaaccattca
    19981 agagtcaatt ggcgcgcatt atacgctgaa attaatccaa aaggaaaaca aaaacagcgt
    20041 ttcaaaaaat atacaacttg ttagacaata aattgttatc aaagatttaa cgttccaggg
    20101 aaaaggttac gcaaaaactt cgctgtttgc gcaacgtctg gtgccgaagt tgtcagtgtg
    20161 aggataagtg aaagttcatc cggtgtataa atatatcatc gctcaattgc tacagttaaa
    20221 ataatttatc gcgttatttg tctgattttg tttgattttt gtttaattgg ttagattgat
    20281 atatgctgat a
//
LOCUS       DAZXUL010000074        20234 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZXUL010000074 DAZXUL010000000
VERSION     DAZXUL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20234
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.074"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(435..641)
                     /locus_tag="ACH3L1_002667"
     CDS             complement(435..641)
                     /locus_tag="ACH3L1_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFT4077356.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="ACH3L1_002668"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="ACH3L1_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFT4077357.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="ACH3L1_002669"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="ACH3L1_002669"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFT4077358.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4489
                     /gene="kdpB"
                     /locus_tag="ACH3L1_002670"
                     /pseudo
     CDS             2739..4489
                     /gene="kdpB"
                     /locus_tag="ACH3L1_002670"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="ACH3L1_002671"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="ACH3L1_002671"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFT4077359.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="ACH3L1_002672"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="ACH3L1_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8722
                     /gene="kdpE"
                     /locus_tag="ACH3L1_002673"
                     /pseudo
     CDS             8037..8722
                     /gene="kdpE"
                     /locus_tag="ACH3L1_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8884..9018
                     /locus_tag="ACH3L1_002674"
     CDS             8884..9018
                     /locus_tag="ACH3L1_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFT4077360.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA
                     "
     gene            8997..9101
                     /gene="speFL"
                     /locus_tag="ACH3L1_002675"
     CDS             8997..9101
                     /gene="speFL"
                     /locus_tag="ACH3L1_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFT4077361.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9412..11609
                     /gene="speF"
                     /locus_tag="ACH3L1_002676"
                     /pseudo
     CDS             9412..11609
                     /gene="speF"
                     /locus_tag="ACH3L1_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11606..12925
                     /gene="potE"
                     /locus_tag="ACH3L1_002677"
     CDS             11606..12925
                     /gene="potE"
                     /locus_tag="ACH3L1_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFT4077362.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="ACH3L1_002678"
     CDS             complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="ACH3L1_002678"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFT4077363.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="ACH3L1_002679"
     CDS             complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="ACH3L1_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFT4077364.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15377..16141
                     /gene="ybfF"
                     /locus_tag="ACH3L1_002680"
     CDS             15377..16141
                     /gene="ybfF"
                     /locus_tag="ACH3L1_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFT4077365.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16281..16574
                     /gene="ybfE"
                     /locus_tag="ACH3L1_002681"
     CDS             16281..16574
                     /gene="ybfE"
                     /locus_tag="ACH3L1_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFT4077366.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16714..17244
                     /gene="fldA"
                     /locus_tag="ACH3L1_002682"
     CDS             16714..17244
                     /gene="fldA"
                     /locus_tag="ACH3L1_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFT4077367.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17454..17540
                     /gene="uof"
                     /locus_tag="ACH3L1_002683"
     CDS             17454..17540
                     /gene="uof"
                     /locus_tag="ACH3L1_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFT4077368.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17533..17979
                     /gene="fur"
                     /locus_tag="ACH3L1_002684"
     CDS             17533..17979
                     /gene="fur"
                     /locus_tag="ACH3L1_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFT4077369.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18063..18389)
                     /gene="chiQ"
                     /locus_tag="ACH3L1_002685"
     CDS             complement(18063..18389)
                     /gene="chiQ"
                     /locus_tag="ACH3L1_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFT4077370.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="ACH3L1_002686"
     CDS             complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="ACH3L1_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFT4077371.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         4703 a         5046 c         5349 g         5136 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgttaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8461 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8521 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8581 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8641 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8701 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt
     8761 tattattaaa cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat
     8821 aaaacacgaa attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa
     8881 ttaatgattt attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc
     8941 catttcatgg atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg
     9001 aaaataataa ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc
     9061 atcgtaatag ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac
     9121 cccaacgtac aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg
     9181 ctcatatact gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg
     9241 aggtatgacg agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca
     9301 aactactgat ttatttatca gcggtggagc tttgcttttt ttccgacgtg atcgatttct
     9361 cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa
     9421 ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg
     9481 aatattgacg aaagtaatta tattgacgtt gccgccatta ttttatcagt caatgatgtt
     9541 gaacgtggaa aactcgataa aatagacgct actggctatg gcattcctgt ttttattgca
     9601 acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg tgtcttcgag
     9661 cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc tagccattat
     9721 gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggcaat
     9781 agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgtca tccggcaggg
     9841 aatcagttcg tggagtactt cggagaaacg ctgttccgtt ccgacttatg caacgcagac
     9901 gtagcaatgg gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg
     9961 gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac
    10021 aaagtggttt taaacgccct gctaacaccg ggtgatctgg tgctgtttga tcgcaataac
    10081 cacaaatcta accaccacgg agcgttgcta caggctggtg caacaccggt ttatctggaa
    10141 acggcacgta acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt
    10201 tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc
    10261 cgcctcgctg tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg
    10321 gtggataaaa ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat
    10381 gagcaattta tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac
    10441 gatccgggta ttctagttac gcaatccgta cataaacagc aggctggttt ttctcaaact
    10501 tcacaaatcc ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa
    10561 cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc
    10621 gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat
    10681 tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt
    10741 ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
    10801 gcggttgatc tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc
    10861 tatgcagaga atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt
    10921 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
    10981 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta acgccatcct cttcctgctg
    11041 actccggcag aagatatggc caaacttcag caacttgtgc cctgctggta cgcttcgaaa
    11101 aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg
    11161 aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg
    11221 cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg
    11281 tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc
    11341 gtctaccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg
    11401 tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc
    11461 tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg
    11521 aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa
    11581 gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt
    11641 tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac
    11701 aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc
    11761 aatggcactg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat
    11821 gggcggttat gccgagtatg ctttcggtaa atccggtaac tttattgcga actataccta
    11881 cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac
    11941 cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct
    12001 gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat
    12061 taccgtgtgg gggggcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt
    12121 tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt
    12181 aggttcttcc atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa
    12241 tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt
    12301 aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat
    12361 ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt ttacaccaga
    12421 agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg
    12481 gcagttcacc attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat
    12541 tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat
    12601 ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct
    12661 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
    12721 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc
    12781 ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct
    12841 gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt
    12901 tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc
    12961 tcttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct
    13021 tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat
    13081 gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga
    13141 ccgccaatag aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacct
    13201 gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg
    13261 gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg
    13321 ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac
    13381 ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc
    13441 cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat
    13501 ttgaaaccta ccggtacttc taccagctta cggcccaagt cattgaccac acggtcgatc
    13561 atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc
    13621 tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga
    13681 gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca
    13741 cgcagtgcca gcagacccgc catcgcacac tcggaggagc agtccatacg gatcgcaccg
    13801 tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg
    13861 ttgaggttgt aatactcgcc aatgcgcttc cagtattcga taccggaacc gcccaacgga
    13921 tcaacgccca gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggccagc
    13981 ccttccacga acggctgcac cagatcctgc tctttcacat agccggatgc catcgcttcg
    14041 tcgagggaga tacgcttcac gcctttcagt ccatcggcca tcagtgcgtt ggccttgtct
    14101 tccaccactt tggtgacgtt agtatcagcc gggccaccat ttggcggatt gtacttgata
    14161 ccaccatctt ccggcgggtt atgggacggt gtaatcacga taccgtctgc cagcgggcca
    14221 ccttttttat tgtgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg
    14281 ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac
    14341 gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt
    14401 ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg
    14461 tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc
    14521 cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca
    14581 ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc
    14641 aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg
    14701 catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata
    14761 cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc
    14821 atctgccgca aagtacacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc
    14881 ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt
    14941 ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat
    15001 cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac
    15061 cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc
    15121 gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc
    15181 aatcgttttc atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caagagtata
    15241 tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac
    15301 cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga
    15361 aaaaaagaac tttgccatga aattaaatat ccgcgcgcaa actgcacaaa accagcacaa
    15421 taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact
    15481 ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct
    15541 ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg caggatcttg ttgataccct
    15601 ggatgcactg cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt
    15661 aatggcactt actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc
    15721 accggtcgac tatcacgtac gccgtcatga tgagattttc gcagctatca acgcggtcag
    15781 tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga
    15841 agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt
    15901 gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga
    15961 tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga
    16021 tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg
    16081 ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta
    16141 acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac
    16201 tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt
    16261 cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt
    16321 tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc
    16381 tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg
    16441 aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgtaata
    16501 tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc
    16561 agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg
    16621 attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc
    16681 catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc
    16741 agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac
    16801 gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttacgacatt
    16861 ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc
    16921 ttcccgactc tcgaagagat tgatttcaat ggtaaactgg ttgcgctgtt tggttgtggt
    16981 gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt
    17041 gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca
    17101 tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag
    17161 ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat
    17221 ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta
    17281 atagataatg ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctata
    17341 caatgtccca gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat
    17401 taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac
    17461 gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag
    17521 gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg
    17581 cttcctcgtt taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg
    17641 gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat
    17701 cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt
    17761 aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatttg cctcgactgc
    17821 ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca
    17881 aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc
    17941 gattgccgcg aagatgagca cgcgcacgaa ggcaaataag ccagcctgaa cgagaaaagc
    18001 caacctgcgg gttggctttt ttatacaagg gaaataagaa aagcgagagt tacagctctc
    18061 acttatttgt tattactgcg tatttcctgc cagaccttat cacaatcggc tttcaccgcc
    18121 tgatcatttc cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcgtacactt
    18181 tcctggtcgg caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg
    18241 caggcttcaa ttttttctgg cgaaccttgc gcggtgttga tacaggcgct gtatgcctct
    18301 ttcagacggc tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc
    18361 atcatggcga tgagaatcag ttttttcatt gaatgcggca ggcacaaaac ctgccgcgtc
    18421 gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg ctcatcctgg
    18481 aagatgttac cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg
    18541 aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc
    18601 aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc
    18661 tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct
    18721 ggaagattcc agtttttcag gtcatacatc gcactgaaga agaccgcttt ttcgccgtta
    18781 gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga ggaagcgtag
    18841 gttggggtca tacgttgcag gaagtatccc tgctgactgt cagccttaat ccaggtgcct
    18901 tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag
    18961 gcggtgccgt cataaagatc gttgacgctg cgatcgtcaa ctttatcgcg ggtaccatag
    19021 aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca
    19081 aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac gaagttattt
    19141 ttgaagtcgt atttcgcacc gatggagtgc agataatcaa ctttggtggt tttatcgttc
    19201 tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt ccacatgtag
    19261 gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgccccctga
    19321 taagtacccg gcataaagct ccagtgcggc gctaacagcg tttgaccggt tggctgaatg
    19381 taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt atacaggctt
    19441 ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg
    19501 atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt aaacgcagca
    19561 atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag
    19621 gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt
    19681 tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg
    19741 cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcaat agccagcgcc
    19801 agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg
    19861 ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    19921 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    19981 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    20041 gtttgcggtt gaaaggcttg cagaagaata aaaacgtgaa attccagcaa gatgaaagcg
    20101 gttacatttt atttaattat gttttgtgat tttaaattta ttttatggat aaattgcgaa
    20161 gcggaattaa ttcgcgaaga aaagcagaaa aaacgccgca gagcgcggcg tttgggattg
    20221 tttgctattt cttg
//
LOCUS       DAZXUL010000075        20203 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZXUL010000075 DAZXUL010000000
VERSION     DAZXUL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20203
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.075"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..579
                     /locus_tag="ACH3L1_002687"
     CDS             <1..579
                     /locus_tag="ACH3L1_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFT4077372.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="ACH3L1_002688"
     CDS             597..1235
                     /locus_tag="ACH3L1_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFT4077373.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="ACH3L1_002689"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="ACH3L1_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFT4077374.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACH3L1_002690"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACH3L1_002690"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFT4077375.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="ACH3L1_002691"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="ACH3L1_002691"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFT4077376.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACH3L1_002692"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACH3L1_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFT4077377.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="ACH3L1_002693"
     CDS             complement(5552..6613)
                     /locus_tag="ACH3L1_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFT4077378.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="ACH3L1_002694"
     CDS             complement(6610..8007)
                     /locus_tag="ACH3L1_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFT4077379.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="ACH3L1_002695"
     CDS             8163..9161
                     /locus_tag="ACH3L1_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFT4077380.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACH3L1_002696"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACH3L1_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="ACH3L1_002697"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="ACH3L1_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACH3L1_002698"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACH3L1_002698"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFT4077381.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACH3L1_002699"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACH3L1_002699"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFT4077382.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15286)
                     /locus_tag="ACH3L1_002700"
                     /pseudo
     CDS             complement(14232..15286)
                     /locus_tag="ACH3L1_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(15296..16071)
                     /locus_tag="ACH3L1_002701"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="ACH3L1_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(16089..17141)
                     /locus_tag="ACH3L1_002702"
     CDS             complement(16089..17141)
                     /locus_tag="ACH3L1_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFT4077383.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="ACH3L1_002703"
     CDS             complement(17172..18014)
                     /locus_tag="ACH3L1_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFT4077384.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="ACH3L1_002704"
     CDS             complement(18001..18882)
                     /locus_tag="ACH3L1_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFT4077385.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="ACH3L1_002705"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="ACH3L1_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4908 a         5537 c         4945 g         4813 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tat
//
LOCUS       DAZXUL010000076        20074 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZXUL010000076 DAZXUL010000000
VERSION     DAZXUL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20074
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.076"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..89)
                     /locus_tag="ACH3L1_002706"
     CDS             complement(<1..89)
                     /locus_tag="ACH3L1_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077386.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLG"
     gene            245..1741
                     /gene="ravA"
                     /locus_tag="ACH3L1_002707"
     CDS             245..1741
                     /gene="ravA"
                     /locus_tag="ACH3L1_002707"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFT4077387.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1735..3186
                     /gene="viaA"
                     /locus_tag="ACH3L1_002708"
     CDS             1735..3186
                     /gene="viaA"
                     /locus_tag="ACH3L1_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFT4077388.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3191..4183)
                     /gene="asnA"
                     /locus_tag="ACH3L1_002709"
     CDS             complement(3191..4183)
                     /gene="asnA"
                     /locus_tag="ACH3L1_002709"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFT4077389.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4335..4793
                     /gene="asnC"
                     /locus_tag="ACH3L1_002710"
     CDS             4335..4793
                     /gene="asnC"
                     /locus_tag="ACH3L1_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFT4077390.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4883..5326
                     /gene="mioC"
                     /locus_tag="ACH3L1_002711"
     CDS             4883..5326
                     /gene="mioC"
                     /locus_tag="ACH3L1_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFT4077391.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5705..7594
                     /gene="mnmG"
                     /locus_tag="ACH3L1_002712"
     CDS             5705..7594
                     /gene="mnmG"
                     /locus_tag="ACH3L1_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFT4077392.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7658..8281
                     /gene="rsmG"
                     /locus_tag="ACH3L1_002713"
     CDS             7658..8281
                     /gene="rsmG"
                     /locus_tag="ACH3L1_002713"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFT4077393.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8898..9278
                     /gene="atpI"
                     /locus_tag="ACH3L1_002714"
     CDS             8898..9278
                     /gene="atpI"
                     /locus_tag="ACH3L1_002714"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFT4077394.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9287..10102
                     /gene="atpB"
                     /locus_tag="ACH3L1_002715"
     CDS             9287..10102
                     /gene="atpB"
                     /locus_tag="ACH3L1_002715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFT4077395.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            10149..10388
                     /gene="atpE"
                     /locus_tag="ACH3L1_002716"
     CDS             10149..10388
                     /gene="atpE"
                     /locus_tag="ACH3L1_002716"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFT4077396.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10450..10920
                     /gene="atpF"
                     /locus_tag="ACH3L1_002717"
     CDS             10450..10920
                     /gene="atpF"
                     /locus_tag="ACH3L1_002717"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFT4077397.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10935..11468
                     /gene="atpH"
                     /locus_tag="ACH3L1_002718"
     CDS             10935..11468
                     /gene="atpH"
                     /locus_tag="ACH3L1_002718"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFT4077398.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11481..13022
                     /gene="atpA"
                     /locus_tag="ACH3L1_002719"
     CDS             11481..13022
                     /gene="atpA"
                     /locus_tag="ACH3L1_002719"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFT4077399.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            13073..13936
                     /gene="atpG"
                     /locus_tag="ACH3L1_002720"
     CDS             13073..13936
                     /gene="atpG"
                     /locus_tag="ACH3L1_002720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFT4077400.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13963..15345
                     /gene="atpD"
                     /locus_tag="ACH3L1_002721"
     CDS             13963..15345
                     /gene="atpD"
                     /locus_tag="ACH3L1_002721"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFT4077401.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15366..15785
                     /locus_tag="ACH3L1_002722"
     CDS             15366..15785
                     /locus_tag="ACH3L1_002722"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFT4077402.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            16136..17506
                     /gene="glmU"
                     /locus_tag="ACH3L1_002723"
     CDS             16136..17506
                     /gene="glmU"
                     /locus_tag="ACH3L1_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFT4077403.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17668..19497
                     /gene="glmS"
                     /locus_tag="ACH3L1_002724"
     CDS             17668..19497
                     /gene="glmS"
                     /locus_tag="ACH3L1_002724"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFT4077404.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19659..19889
                     /locus_tag="ACH3L1_002725"
                     /pseudo
     CDS             19659..19889
                     /locus_tag="ACH3L1_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
     gene            19959..>20074
                     /locus_tag="ACH3L1_002726"
     CDS             19959..>20074
                     /locus_tag="ACH3L1_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFT4077405.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTA"
BASE COUNT         4979 a         5009 c         5279 g         4807 t
ORIGIN      
        1 cccagagaag tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt
       61 ccaatgtgca taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact
      121 ggatcggtca actgatcctt aactgatcgg tattaccccc ctttgagact attttagtca
      181 ccagcaagaa acgtctatac tcgcaattta cgcagaactt ttgacgaaag gacgccactt
      241 cattatggct caccctcatt tattagcgga aagaatttcc cgcctgagca gttcgttgga
      301 aaaggggctt tatgaacgta gccacgccat ccgcttgtgt ttattagcgg cattaagtgg
      361 tgaaagtgtg ttcctccttg gcccgccagg tattgccaaa agtttgatcg cccggcgctt
      421 aaaattcgcc tttcagaatg cccgcgcgtt tgaatatctg atgacccgct tctccacgcc
      481 ggaagaagtt tttggtcccc tttctattca ggcgctaaaa gatgaagggc gctatgaacg
      541 tttaaccagc ggttacctgc cggaagcaga aatcgtcttt ctggatgaga tctggaaagc
      601 gggcccggca attcttaata ccttgctcac cgccattaac gagcgccagt tccgcaacgg
      661 tgcacacgta gaaaaaatcc cgatgcgtct actggtggcg gcctccaacg agctgccgga
      721 agcagacagc agtctggaag cgttatatga ccgcatgctg attcgtctat ggttagataa
      781 agtgcaagat aaagcgaatt tccgctccat gctgaccagt caacaggatg aaaacgacaa
      841 tccggttcct gatgccttgc aggtcacaga tgaagaatat gaacgctggc agaaagagat
      901 tggtgaaatt acgctgcccg atcatgtatt tgagctgatt tttattctgc gccagcaact
      961 ggataaatta ccggatgcgc cttatgtctc ggatcgtcgc tggaaaaaag cgatccgctt
     1021 attgcaggcc agcgcctttt ttagcggtcg cagtgctgtt gccccggttg atctcatttt
     1081 gctgaaagat tgcctgtggt atgacgcgca aagcctgaat ttgatacaac aacaaattga
     1141 tgtattgatg accggtcatg cctggcaaca gcaagggatg ttgacccgcc tgggcgcgat
     1201 tgtgcaacgt cacctgcaac tccagcagca acaaagcgat aaaacagcct taacgttaat
     1261 tcgtctgggc ggcattttca gccgtcgtca gcagtatcaa cttcctgtta acgttactgc
     1321 ttccactctg actctgctgc tgcaaaaacc gttaaaactg catgatatgg aagtggttca
     1381 tatctccttt gagcgtagcg cgctggaaca gtggctgagt aaaggtggtg aaattcgcgg
     1441 caaactgaac ggtatcggct ttgcccagaa actgaatctg gaagttgata gcacccaaca
     1501 tcttgttgta cgcgatgtaa gtttacaagg cagtacgctg gcacttcccg gttcatcggc
     1561 tgaaggtctg ccaggtgaaa taaaacaaca actggaagag cttgaaagcg actggcgtaa
     1621 gcaacacgct ttattcagcg aacagcaaaa atgtctgttt atccctggcg actggttagg
     1681 tcgcattgaa gacagcctac aggatgtcgg tgcacagatt cgtcaggcac aacaatgcta
     1741 acgctggata cgcttaatgt gatgctggcc gtcagcgaag agggattgat cgaagagatg
     1801 atcatcgcac tgctggcctc accgcagctg gcggtcttct ttgaaaaatt cccacgactg
     1861 aaggcggcaa tcactgatga tgttccccgc tggcgtgagg cgctgcgcag tcggctgaaa
     1921 gatgcccgag tcccgccgga actcaccgaa gaggtgatgt gctatcagca aagccagctc
     1981 ctctccacgc cgcagtttat tgtgcagcta ccacagatcc tggacttact gcatcgtctg
     2041 aattccccat gggcagaaca agcccggcag ttggttgatg ctaacagcac aataacttca
     2101 gcgttacaca cgctttttct ccagcgttgg cgtttaagtc tgatcgtgca agcaacgacg
     2161 ttaaatcaac agctattaga agaagaacgc gaacaactgt taagtgaagt tcaggaacgc
     2221 atgacgctga gcggacaact tgaaccgatt ctcgcagata acaataccgc agctggtcgt
     2281 ctgtgggata tgagcgccgg tcaacttaaa cgtggcgact atcagtttat tgtgaaatac
     2341 ggtgaatttc ttaacgaaca gccggaactg aaacgcctgg cagagcagct ggggcgttct
     2401 cgggaagcca aatcaatacc gcgcaacgat gcgcagatgg aaaccttccg caccatggtg
     2461 cgcgaaccgg cgacggttcc tgagcaggtt gatggtctgc aacaaagcga tgatatttta
     2521 cgtctcctgc cgccagaact ggcgacacta gggataacgg aactggagta tgagttttac
     2581 cgtcggctgg tggaaaaaca gttgctcacc tatcgcctgc acggtgagtc gtggcgtgaa
     2641 aaagtgttcg aacgtccggt ggtacataaa gattacgatg aacagccgcg cgggccgttt
     2701 attgtctgtg tggatacttc cggctcaatg ggcggcttta atgaacagtg tgcgaaagcg
     2761 ttctgcctgg ctttgatgcg cattgctctc gctgaaaacc ggcgctgtta tattatgcta
     2821 ttttccaccg agatcgtccg ttatgagctt tcaggcccac aaggcatcga acaagcaatc
     2881 cgttttttaa gccagcagtt tcgtggcggc accgatcttg ccagttgttt tcgcgccatt
     2941 atggaacgct tgcaaagcag ggaatggttt gatgccgatg cggtggtgat ttctgatttt
     3001 atcgctcagc ggttgcctga cgacgtgacg agtaaagtga aagagctgca gcgggtacat
     3061 cagcatcgct ttcatgccgt ggcgatgtcg gcacacggca aacccggcat catgcgcatt
     3121 ttcgatcata tctggcgctt tgataccggg atgcgaagcc gcctgctcag acgctggcgg
     3181 cgataaatta ttacagcaga gaagggacgc tctcgcgaac agcagctggc catactccac
     3241 actgaacctg gccgatatgc ggcagttgca gcagcagcat agtcaaacga gactggccga
     3301 taccgccgcc gatggtctgc ggcatttcac cgcgcagcag cgcctgatgc cattccagct
     3361 gcagacgatc ttcatcaccg gtcagcgcca gctgatgctt cagcgtgtcg gcatccacgc
     3421 ggatccccat ggaagaaagc tcaaacgcat cttccagtac cgggttccac accagaatat
     3481 cgccgttcag gcccgcatgg cccagctctg acggggtgct ccagtcatca taatccggtg
     3541 cgcgcacgtc gtgacgatga ccatcactca acttgccgcc aatcccgacg aggaataccg
     3601 cgccaagatc tttcgcaatc gcccgctcac gccctttggc atcgagatcc ggataacgag
     3661 acagtaactc ctggctgtgt acgaagtgaa tctgatccgg caggaacggt gccaggccaa
     3721 actcttcgtt aaccgcagct tcggttgctt taattcccgc ccagatcgcc tctaccgtgc
     3781 ttttcagagt cgagagttga cgctcaccgt cgcccattac gcgttcccag tcccactggt
     3841 caacatagac cgagtgcaac ggagaaagac ggtcttcatc ggggcgaagg gctttcatgt
     3901 gcgtgtacag cccttcgccc gcgctgaagt cgtgttgccc taaggtctga cgtttccact
     3961 tcgccagtga atgaaccact tcgaactggg catcaggcag agctttcact tttacctgca
     4021 ccgctttttc acagcccgac aagttatcct gcgtgccatc ccccacacgg ctaagaatcg
     4081 gcgcctggac ttcgatcagc cccagacgtt cttccagttg gcgagaaaag tgagatttca
     4141 cgaagctaat ttggcgttgt ttggcaatgt aagcggtttt cattttttat actcctgcgt
     4201 cctgttgctt atgattaagc aacaaaaacc gacacacatg caataatcat tcaataaaaa
     4261 gcctaacaca cttttgattc attaaaaaag acggctaaaa tagaatgaat catcaatctg
     4321 cataagaaaa tcctatggaa aattatctga tcgacaatct ggaccgtggc atcctggaag
     4381 cattaatggg caatgcgcgc accgcttacg ccgaactggc gaaacaattt ggcgtcagtc
     4441 cggggacgat tcacgttcga gtagagaaaa tgaagcaggc ggggatcatt accggggcgc
     4501 gtattgatgt cagcccgaag cagctcggtt atgacgtagg ctgctttatc ggcattatat
     4561 taaagagcgc caaagactac ccttccgcgc tggcaaagct ggaaagcctt gatgaagtca
     4621 ctgaagccta ttacacaacc ggccactaca gcatctttat aaaagttatg tgccgttcga
     4681 tcgacgctct ccagcatgta cttatcaaca agatccaaac aattgatgaa attcagtcca
     4741 ccgagacatt gatcgtcctg caaaacccga tcatgcgtac catcaagccc tgatcggctt
     4801 ttttaatccc atacttttcc acaggtagat cccaacgcgt tcacagcgta caatacgcca
     4861 ctcttaataa aggtggcggt ttatggcaga tatcactctt atcagcggca gcaccctcgg
     4921 cggtgccgaa tatgtagcag aacacctggc tgaaaagctg gaagaggtgg gttttaccac
     4981 cgaaacgctg cacggtccgc tgttggaaga tttatctgcc tcggggatct ggctggttat
     5041 cagctccacc cacggtgccg gagatattcc ggacaacctt tctcctttct atgaagcatt
     5101 gcaggaacag aagcccgatc tttctgcggt ccgctttggc acaatcggta ttggcagtcg
     5161 tgaatatgac accttttgtg gggctatcga taaactcgag gcagaactca aaaattccgg
     5221 tgcaaaacag acaggcgaaa cactgaagat caacattctt gatcacgaca ttccggaaga
     5281 tccggcagaa gaatggctgg gatcgtggat taatttactc aaataagtat acagatcgtg
     5341 cgatctactg tggataactc tgtcaggaag cttggatcaa ccggtagcta tccaaagaac
     5401 aaccgttgtt cagtttttga gttgtgtata acccctcatt ctgatcccag cttatacggt
     5461 ccaggatcac cgatcattca cagttaatga tcctttccag gttgttgatc ttaaaaatcg
     5521 gatccttgtt atccacaggg cagtgcgatc ctaataagag atcacaatag aacagatctc
     5581 taaataaata gatcttcttt ttaataccca ggatcccagg tctttctcaa gccgacaaag
     5641 ttgagtagaa tccacggccc gggcttcaat ccattttcat accgcgttat gcgaggcaat
     5701 caccatgttt tatccggatc cttttgacgt catcatcatt ggcgggggtc atgcaggcac
     5761 cgaggccgcg atggctgcgg cgcgtatggg tcaacagact ctgcttttga cacacaatat
     5821 cgacactctg gggcagatga gctgcaaccc ggcgatcggc ggtattggga agggacatct
     5881 ggtaaaagaa gtggatgcac tcggcggtct gatggcgaaa gcgatcgatc aagcgggtat
     5941 ccagtttagg atactaaacg caagcaaagg accggcggtt cgcgctaccc gagctcaggc
     6001 ggatcgtgtg cgctaccgtc aggcggtacg tacggcgctg gagaaccaac cgaacctgat
     6061 gatcttccag caggcggttg aagatcttat tgtcgaaaac gatcgcgtgg tcggtgctgt
     6121 tacccaaatg ggactgaagt tccgtgccaa agctgtggtg ctcaccgttg ggacgttcct
     6181 cgacggcaaa attcatatcg gtctggataa ttacagcggt ggccgtgctg gcgatccgcc
     6241 atccattccg ctctctcgcc gtttgcgtga actgccgctg cgcgttggtc gtctgaaaac
     6301 cggtactccg ccgcgtattg atgctcgaac catcgacttt agcgtgctgg cgcaacagca
     6361 tggcgataac ccaatgccgg tattctcgtt tatgggcaat gcgtccctgc atccccagca
     6421 ggtgccgtgt tatatcactc ataccaacga gaaaacccat gatgtgatcc gcagtaacct
     6481 cgatcgtagc ccaatgtatg caggggtgat cgaaggtgtc ggcccacgct actgcccgtc
     6541 gatcgaagac aaagtcatgc gcttcgcaga cagaaatcag catcagatct tccttgaacc
     6601 ggaaggactg acctctaatg aaatttatcc gaacggtatc tccaccagcc tgccgttcga
     6661 tgtgcagatg caaatcgtcc gctctatgca ggggatggaa aacgcgaaga tcgtgcgtcc
     6721 gggttatgcc attgagtatg acttcttcga tcctcgcgac ctgaaaccga cgctggagag
     6781 caagtttatc caggggctgt tctttgctgg tcagattaac ggcactaccg gttacgaaga
     6841 agccgctgcg caaggtttgc tggccggtct taacgctgcc cgtctgtctg ctgacaaaga
     6901 aggttgggct ccggcacgtt ctcaggcgta tctcggcgta ctggttgatg acctgtgcac
     6961 tttaggaacc aaagaaccgt atcgtatgtt tacttcgcgc gcagaatatc gtctgatgct
     7021 gcgcgaagat aatgcggatc tgcgtttgac tgaaatcggt cgtgaactgg gcctggtgga
     7081 tgacgaacgt tgggcgcgct ttaacgagaa acttgagaat atcgagcgtg agcgtcagcg
     7141 tctgaaatcg acctgggtaa ccccgtcggc ggaagctgca gccgaagtga atgctcacct
     7201 gactgcgccg ctttcccgtg aagccagtgg tgaagatctg ctgcgtcgtc cggaaatgac
     7261 ttatgaaaaa ttaaccacgc tgacgccgtt tgcccctgcg ttgacagacg aacaggcggc
     7321 ggaacaggtt gagattcagg ttaaatacga aggttatatc gcgcgccagc aagatgagat
     7381 cgaaaagcag ctgcgtaacg agaacaccct gctacccgcg acactggatt accgccagat
     7441 atccggtctt tctaacgaag tgatcgccaa acttaacgat cacaaaccgg cctctatcgg
     7501 ccaggcttcg cgtatttctg gcgtcacgcc tgcggccatc tccattctgc tggtgtggct
     7561 gaaaaaacag ggtatgctgc gtcgtagcgc ataacgcatt aaaaatgcct ggtaagcacc
     7621 cgcttaccag gcaacgcatc aagaacaggt aatcaccgtg ctcaacaaac tctccttact
     7681 gctgaaagac gcaggtattt cgcttactga tcaccagaaa aaccagctta ttgcctacgt
     7741 gaatatgctg cataaatgga acaaagcgta caacctgact tcggtccgcg atcctaatga
     7801 gatgctggta cgccatattc tcgatagcat tgtggtggca ccgtatctgc aaggtgaacg
     7861 gtttatcgat gtcggcaccg gaccaggact gccaggcatt ccactctcta tcgtgcgtcc
     7921 tgaagcccat ttcactctgt tggatagcct tggtaaacgc gtgcgtttcc ttcgtcaggt
     7981 gcaacatgag cttaaactgg agaatattga accagtacag agcagggtag aagagtttcc
     8041 ttcagagccg ccatttgatg gcgtaattag ccgcgctttt gcctctctga acgatatggt
     8101 gagctggtgc caccatcttc ctggtgagca aggccgtttc tacgcgctga aagggcaaat
     8161 gccggaagat gaaatcgctt tgttgcccga agaatatcag gtcgaatcag tggttaaact
     8221 tcaggttcca gccctggatg gcgaacgtca tctggtggtg attaaagcaa ataaaattta
     8281 atttttatca aaaaaatcat aaaaaattga ccggttagac tgttaacaac aaccaggttt
     8341 tctactgata taactggtta catttaacgc cacgttcact cttttgcatc aacaagataa
     8401 cgtggctttt tttggtaagc agaaaataag tcattagtga aaatatcagt ctgctaaaaa
     8461 tcggcgctaa gaaccatcat tggctgttaa aacattatta aaaatgtcaa tgagtggttt
     8521 ttgttgtgta aatgttattt attaaaacag tatctgtttt tagactgaaa tatcataaac
     8581 ttgtaaaggc atcatttgcc aagtaaataa atatgctgtg cgcgaacatg cgcaatatgt
     8641 gatctgaagc acgctttatc accagtgttt acgcgttatt tacagttttt catgatcgaa
     8701 caggggtagc ggaaaagtcg caattgtatg cactggaaaa atatttaaac atttattcac
     8761 cttttggcta cttattgttt gaaatcacgg gggcgcaccg tataatttga ccgctttttg
     8821 atgcttgact ctaagcctta aagaaagttt tatacgacac gcggcatacc tcgaagggag
     8881 caggagtgaa aaacgtgatg tctgtgtcgc tcgtgagtcg aaacgttgct cggaagcttc
     8941 tgctcgttca gttactggtg gtgatagcaa gtggattgct gttcagcctc aaagacccct
     9001 tctggggcgt ctctgcaata agcgggggcc tggcagtctt tctgcctaac gttttgttta
     9061 tgatatttgc ctggcgtcac caggcgcata caccagcgaa aggccgggtg gcttggacat
     9121 tcgcatttgg cgaagctttc aaagttctgg cgatgttggt gttactggtg gtggcgttgg
     9181 cggttttaaa ggcggtattc ttgccgctga tcgttacgtg ggttttggtg ctggtggttc
     9241 agatactggc accggctgta attaacaaca aagggtaaaa ggcatcatgg cttcagaaaa
     9301 tatgacgccg caggattaca taggacacca cctgaataac cttcagctgg acctgcgtac
     9361 attctcgctg gtggatccac aaaacccccc agccaccttc tggacaatca atattgactc
     9421 catgttcttc tcggtggtgc tgggtctgtt gttcctggtt ttattccgta gcgtagccaa
     9481 aaaggcgacc agcggtgtgc caggtaagtt tcagaccgcg attgagctgg tgatcggctt
     9541 tgttaatggt agcgtgaaag acatgtacca tggcaaaagc aagctgattg ctccgctggc
     9601 cctgacgatc ttcgtctggg tattcctgat gaatctgatg gatttactgc ctatcgactt
     9661 gctgccgtac attgctgaac atgtactggg tctgcctgca ctgcgtgtgg ttccgtctgc
     9721 ggacgtgaac gtaacgctgt ctatggcact gggcgtattt atcctgattc tgttctacaa
     9781 catcaaaatg aaaggcatcg gcggcttcac gaaagagttg acgctgcagc cgttcaatca
     9841 ctgggcgttc attcctgtca acttaatcct tgaaggggta agcctgctgt ccaaaccagt
     9901 ttcactcggt ttgcgactgt tcggtaacat gtatgccggt gagctgattt tcattctgat
     9961 tgctggtctg ttgccgtggt ggtcacagtg gatcctgaat gtgccgtggg ccattttcca
    10021 catcctgatc attacgctgc aagccttcat cttcatggtt ctgacgatcg tctatctgtc
    10081 gatggcgtct gaagaacatt aatttaccaa cactactacg ttttaactga aacaaactgg
    10141 agactgtcat ggaaaacctg aatatggatc tgctgtacat ggctgccgct gtgatgatgg
    10201 gtctggcggc aatcggtgct gcgatcggta tcggcatcct cgggggtaaa ttcctggaag
    10261 gcgcagcgcg tcaacctgat ctgattcctc tgctgcgtac tcagttcttt atcgttatgg
    10321 gtctggtgga tgctatcccg atgatcgctg taggtctggg tctgtacgtg atgttcgctg
    10381 tcgcgtagta agcgttgctt ttatttaaag agcaatatca gaacgttaac taaatagagg
    10441 cattgtgctg tgaatcttaa cgcaacaatc ctcggccagg ccatcgcgtt tgtcctgttc
    10501 gttctgttct gcatgaagta cgtatggccg ccattaatgg cagccatcga aaaacgtcaa
    10561 aaagaaattg ctgacggcct tgcttccgca gaacgagcac ataaggacct tgaccttgca
    10621 aaggccagcg cgaccgacca gctgaaaaaa gcgaaagcgg aagcccaggt aatcatcgag
    10681 caggcgaaca aacgccgctc gcagattctg gacgaagcga aagctgaggc agaacaggaa
    10741 cgtactaaaa tcgtggccca ggcgcaggca gaaattgaag ccgagcgtaa acgtgcccgt
    10801 gaagagctgc gtaagcaagt tgctatcctg gctgttgctg gcgccgagaa gatcatcgaa
    10861 cgttccgtgg atgaagctgc taacagcgac atcgtggata aacttgtcgc tgaactgtaa
    10921 ggagggaggg gctgatgtct gaatttatta cggtagctcg cccctacgcc aaagcagctt
    10981 ttgactttgc cgtcgaacac caaagtgtag aacgctggca ggacatgctg acgtttgccg
    11041 ccgaggtaac caaaaacgaa caaatggcag agcttctctc tggcgcgctt gcgccagaaa
    11101 cgctcgccga gtcgtttatc gcagtttgtg gtgagcaact ggacgaaaac ggtcagaacc
    11161 tgattcgggt aatggctgaa aatggtcgtc ttaacgcgct cccggatgtt ctggagcagt
    11221 ttattcacct tcgtgccgtg agtgaggcta ccgctgaggt agacgtcatt tccgctgccg
    11281 cactgagtga acaacagctc gcgaaaattt ctgctgcgat ggaaaaacgt ctgtcacgca
    11341 aagttaagct gaattgcaaa atcgataagt ctgtaatggc tggcgttatc atccgatcgg
    11401 gtgatatggt cattgatggc agcgtacgcg gtcgtcttga gcgccttgca gacgtcttgc
    11461 agtcttaagg ggactggagc atgcaactga attccaccga aatcagcgaa ctgatcaagc
    11521 agcgcattgc tcagttcaat gttgtgagtg aagctcacaa cgaaggtact attgtttctg
    11581 taagtgacgg tgttatccgc attcacggcc tggccgattg tatgcagggt gaaatgatct
    11641 ccctgccggg taaccgttac gctatcgcac tgaacctcga gcgcgactct gtaggtgcgg
    11701 ttgttatggg tccgtacgct gaccttgccg aaggcatgaa agttaagtgt actggccgta
    11761 tcctggaagt tccggttggc cgtggcctgc tgggccgtgt ggttaacact ctgggtgcac
    11821 caatcgacgg taaaggtccg ctggatcacg acggcttctc tgctgtagaa gcaatcgctc
    11881 cgggcgttat cgaacgtcag tccgtagatc agccggtaca gaccggttat aaagccgttg
    11941 actccatgat cccaatcggt cgtggtcagc gtgaattgat catcggtgac cgtcagacag
    12001 gtaaaaccgc actggctatc gatgccatca tcaaccagcg cgattccggt atcaaatgta
    12061 tctatgtcgc tatcggccag aaagcgtcca ccatttctaa cgtggtacgt aaactggaag
    12121 agcacggcgc actggctaac accatcgttg tggtagcaac cgcgtctgaa tccgctgcac
    12181 tgcaatacct ggcaccgtat gccggttgcg caatgggcga atacttccgt gaccgcggtg
    12241 aagatgcgct gatcatttac gatgacctgt ctaaacaggc tgttgcttac cgtcagatct
    12301 ccctgctgct ccgtcgtccg ccaggacgtg aagcattccc gggcgacgtt ttctacctcc
    12361 actctcgtct gctggagcgt gctgcacgtg ttaacgccga atacgttgaa gccttcacca
    12421 aaggtgaagt gaaagggaaa accggttctc tgaccgcact gccgattatc gaaactcagg
    12481 cgggtgacgt ttctgcgttc gttccgacca acgtaatctc cattaccgat ggtcagatct
    12541 tcctggaaac caacctgttc aacgccggta ttcgtcctgc ggttaacccg ggtatttccg
    12601 tatcccgtgt tggtggtgca gcacagacca agatcatgaa aaaactgtcc ggtggtatcc
    12661 gtaccgctct ggcacagtat cgtgaactgg cagcgttctc tcagtttgca tccgaccttg
    12721 acgatgcaac acgtaagcag cttgaccacg gtcagaaagt gaccgaactg ctgaaacaga
    12781 aacagtatgc gccgatgtcc gttgcgcagc agtctctggt tctgttcgca gcagaacgtg
    12841 gttacctggc ggatgttgaa ctgtcgaaaa ttggcagctt cgaagccgct ctgctggctt
    12901 acgtcgaccg tgatcacgct ccgttgatgc aagagatcaa ccagaccggt ggctacaacg
    12961 acgaaatcga aggcaagctg aaaggcatcc tcgattcctt caaagcaacc caatcctggt
    13021 aacgtctgga ggcttgcctt agggcaggcc gcaaggcatt gaggagaagc tcatggccgg
    13081 cgcaaaagag atacgtagta agatcgcaag cgtccagaac acgcaaaaga tcactaaagc
    13141 gatggagatg gtcgccgctt ccaaaatgcg taaatcgcag gatcgcatgg cggccagccg
    13201 tccttatgca gaaaccatgc gcaaagtgat tggtcacctt gcacacggta atctggaata
    13261 taagcaccct tacctggaag accgcgacgt taaacgcgtg ggctacctgg tggtgtcgac
    13321 cgaccgtggt ttgtgcggtg gtttgaacat taacctgttc aaaaaactgc tggcggaaat
    13381 gaagacctgg accgacaaag gcgttcaatg cgacctcgca atgatcggct cgaaaggcgt
    13441 gtcgttcttc aactccgtgg gcggcaatgt tgttgcccag gtcaccggca tgggggataa
    13501 cccttccctg tccgaactga tcggtccggt aaaagtgatg ttgcaggcct acgacgaagg
    13561 ccgtctggac aagctttaca ttgtcagcaa caaatttatt aacaccatgt ctcaggttcc
    13621 gaccatcagc cagctgctgc cgttaccggc atcagatgat gatgatctga aacataaatc
    13681 ctgggattac ctgtacgaac ccgatccgaa ggcgttgctg gataccctgc tgcgtcgtta
    13741 tgtcgaatct caggtttatc agggcgtggt tgaaaacctg gccagcgagc aggccgcccg
    13801 tatggtggcg atgaaagccg cgaccgacaa tggcggcagc ctgattaaag agctgcagtt
    13861 ggtatacaac aaagctcgtc aggccagcat tactcaggaa ctcaccgaga tcgtctcggg
    13921 ggccgccgcg gtttaaacag gttatttcgt agaggattta agatggctac tggaaagatt
    13981 gtccaggtaa tcggcgccgt agttgacgtc gaattccctc aggatgccgt accgcgcgtg
    14041 tacgatgctc ttgaggtgca aaatggtaat gagcgtctgg tgctggaagt tcagcagcag
    14101 ctcggcggcg gtatcgtgcg taccatcgca atgggttcct ccgacggtct gcgtcgcggt
    14161 ctggatgtaa aagacctcga acacccgatc gaagtcccgg taggtaaagc gactctgggc
    14221 cgtatcatga acgtactggg tgaaccggtc gacatgaaag gcgagatcgg tgaagaagag
    14281 cgttgggcga ttcaccgcgc agcaccttcc tacgaagagc tgtcaaactc tcaggaactg
    14341 ctggaaaccg gtatcaaagt tatcgacctg atgtgtccgt tcgctaaggg cggtaaagtt
    14401 ggtctgttcg gtggtgcggg tgtaggtaaa accgtaaaca tgatggagct cattcgtaac
    14461 atcgcgatcg agcactccgg ttactctgtg tttgcgggcg taggtgaacg tactcgtgag
    14521 ggtaacgact tctaccacga aatgaccgac tccaacgtta tcgacaaagt atccctggtg
    14581 tatggccaga tgaacgagcc gccgggaaac cgtctgcgcg ttgctctgac cggtctgacc
    14641 atggctgaga aattccgtga cgaaggtcgt gacgttctgc tgttcgttga caacatctat
    14701 cgttacaccc tggccggtac ggaagtatcc gcactgctgg gccgtatgcc ttcagcggta
    14761 ggttatcagc cgaccctggc ggaagagatg ggcgttctgc aggaacgtat cacctccacc
    14821 aaaactggtt ctatcacctc cgtacaggcg gtatacgtac ctgcggatga cttgactgac
    14881 ccgtctccgg caaccacctt tgcgcacctt gacgcaaccg tggtactgag ccgtcagatc
    14941 gcgtctctgg gtatctaccc ggccgttgac ccgctggact ccaccagccg tcagctggac
    15001 ccgctggtgg ttggtcagga acactacgac accgcgcgtg gcgttcagtc catcctgcaa
    15061 cgttatcagg aactgaaaga catcatcgcc atcctgggta tggatgaact gtctgaagaa
    15121 gacaaactgg tggtagcgcg tgctcgtaag atccagcgct tcctgtccca gccgttcttc
    15181 gtggcagaag tattcaccgg ttctccgggt aaatacgtct ccctgaaaga caccatccgt
    15241 ggctttaaag gcatcatgga aggcgaatac gatcacctgc cggagcaggc gttctacatg
    15301 gtcggttcca tcgaagaagc tgtggaaaaa gccaaaaaac tttaacgcct taatcggagg
    15361 gtgatatggc aatgacttac cacctggacg tcgtcagcgc agagcaacaa atgttctctg
    15421 gtctggtcga gaaaatccag gtaacgggta gcgaaggtga actggggatc taccctggcc
    15481 acgcaccgct gctcaccgcc attaagcctg gtatgattcg catcgtgaaa cagcacggtc
    15541 acgaagagtt tatctatctg tctggcggca ttcttgaagt gcagcctggc aacgtgaccg
    15601 ttctggccga caccgcaatt cgcggccagg atctcgacga agcgcgagcc atggaagcga
    15661 aacgtaaggc tgaagagcac attagcagct ctcacggcga cgtagattac gctcaggcgt
    15721 ctgcggaact ggccaaagcg atcgcgcagc tgcgcgttat cgagttgacc aaaaaagcga
    15781 tgtaacaccg gcttgaaaag cacaaaagcc agtctggaaa caggctggct ttttttacgc
    15841 gttgacccgt cctgaagagc gttcacatag atcctgctga tataaaacac ccctgttttc
    15901 ccgtttattc attgatcgaa ataagagcaa aaacatccac ctgacgctta aatgaaggta
    15961 ctgccttaat tttctgcaga taaaaggcgt gacgatggtc gaaaatggca ctttcgtcag
    16021 cggggataat ccgttattga acaatttatc ctctgtccat ttcacgatga aaaaaatgta
    16081 gttttttcaa ggtgaagcgg tttaaattcg ttctcaaatt acagtcagga cgcgtatgtt
    16141 gaataatgct atgagcgtag tgatccttgc cgcaggcaaa ggcacgcgca tgtattccga
    16201 tcttccgaaa gtgctgcata cccttgccgg gaaagcgatg gttcagcatg tcattgatgc
    16261 tgcgaatgaa ttaggcgcag cgcacgttca cctggtgtac ggtcacggcg gcgatctgct
    16321 taaacaggcg ctgaaagacg acaaccttaa ctgggtgctt caggcagagc agctgggtac
    16381 gggtcatgca atgcagcagg ccgcaccttt ctttgccgat gatgaagaca ttttaatgct
    16441 ctacggcgac gtgccgctga tctctgtcga aacactccag cgcctgcgtg atgctaaacc
    16501 ccagggtggc attggtctgc tgacggtgaa actggatgat ccgaccggtt atggacgtat
    16561 cacccgtgaa aatggcaaag ttaccggcat tgttgagcac aaagatgcca ccgacgagca
    16621 gcgtcagatt caggagatca acaccggcat tctgattgcc aacggcgcag atatgaaacg
    16681 ctggctggcg aagctgacca acaataatgc tcagggcgaa tactacatca ccgacattat
    16741 tgcgctggcg tatcaggaag ggcgtgaaat cgtcgccgtt catccgcaac gtttaagcga
    16801 agtagaaggt gtgaataacc gcctgcaact ctcccgtctg gagcgcgttt accagtccga
    16861 acaggctgaa aaactgctgt tagcaggcgt tatgctgcgc gatccggcgc gttttgatct
    16921 gcgcggtacg cttactcacg ggcgcgatgt tgaaattgat actaacgtta tcatcgaggg
    16981 caacgtgact ctcggtcatc gcgtgaaaat cggcaccggt tgcgtgatta aaaacagcgt
    17041 gattggcgat gattgcgaaa tcagtccgta taccgttgtg gaagacgcga atctggcagc
    17101 ggcctgtact attggcccgt ttgcccgtct gcgtcctggt gctgagttgc tggaaggtgc
    17161 acacgttggt aacttcgttg agatgaaaaa agcgcgtctg ggtaaaggca cgaaagccgg
    17221 gcatctgact tacctgggcg atgcggaaat tggcgataac gttaacatcg gcgcgggaac
    17281 cattacctgc aactacgatg gtgcgaataa atttaagacc attatcggcg acgatgtgtt
    17341 tgtcggttcc gacactcagc tggtggcccc ggtaacagta ggcaaaggcg cgaccattgc
    17401 tgcgggtaca actgtgacgc gtaatgtcgg cgaaaatgca ttagctatca gccgtgtgcc
    17461 gcagactcag aaagaaggct ggcgtcgtcc ggtaaagaaa aagtaattct ggccgggtaa
    17521 tccggtcatc tgggatgagg agataacata atctccctcc cacaagcagt aactataaaa
    17581 ataaccccac tctctacaag gctcggggcg cccgaaaaaa cgggcataca ggttgaccga
    17641 caacgatata aatcggaatc aaaaactatg tgtggaattg ttggcgcgat cgcgcaacgt
    17701 gatgtagcag aaatccttct tgaaggttta cgtcgtctgg aataccgcgg atatgactct
    17761 gccggtctgg ccgttgttga tgcggaaggt catatgactc gcctgcgtcg cctcggtaaa
    17821 gtccagatgc tggctcaggc agcggaagaa catcctctgc atggcggcac cggtattgct
    17881 cacactcgct gggcgaccca cggtgaacct tcagaagcta acgcgcatcc gcatgtttct
    17941 gaacacattg tggtggtgca taatggcatc atcgaaaacc atgaaccgct gcgtgaagag
    18001 ttaaaagcgc gtggctatac cttcgtttct gaaaccgaca ccgaagtgat tgcccatctg
    18061 gtgaactggg agctgaaaca aggcgggact ctgcgtgagg ctgttctgcg tgctatcccg
    18121 cagctgcgcg gtgcgtacgg tacagtgatc atggactccc gtcacccgga taccctgcta
    18181 gcggcacgtt ctggtagtcc gctggtgatt ggcctgggta tgggcgaaaa ctttatcgct
    18241 tctgaccagc tggcgctgtt gccggtgacc cgtcgcttta tcttccttga agagggcgat
    18301 attgcggaaa tcactcgccg ttcggtaaac atcttcgata aaactggcgc ggaagtaaaa
    18361 cgtcaggata tcgaatccaa tctgcaatat gacgcgggcg ataaaggtat ttaccgtcac
    18421 tacatgcaga aagagatcta cgaacagccg aacgcgataa aaaacaccct taccgggcgc
    18481 atcagccacg gtcaggttga tttaagcgag ctgggaccga acgccgacga actgctgtcg
    18541 aaggttgagc atattcagat cctcgcctgt ggtacttctt ataactccgg tatggtttcc
    18601 cgctactggt ttgaatcgct ggcaggtatt ccgtgcgacg tcgaaatcgc ctctgaattc
    18661 cgctatcgta aatctgccgt gcgtcgtaac agcctgatga tcaccttgtc acagtctggc
    18721 gaaaccgcgg ataccctggc gggcctgcgt ctgtcgaaag agctgggtta ccttggttcg
    18781 ctggcaatct gtaacgttcc gggttcttct ctggtgcgcg aatccgatct ggcgctgatg
    18841 accaacgcgg gtacggaaat cggcgtggcg tccactaaag cattcaccac tcagttaact
    18901 gtgctgttga tgctggtggc gaagctgtct cgcctgaaag gtctggatgc ctccattgaa
    18961 catgacattg tgcatggtct gcaggcgctg ccgagccgta ttgagcagat gctgtctcag
    19021 gataaacgca ttgaagctct ggcagaagat ttctctgaca aacatcacgc gctgttcctg
    19081 ggccgtggcg atcagtaccc aatcgcgctg gaaggcgcat tgaagctgaa agagatctct
    19141 tacattcacg ctgaagccta cgctgcaggc gagctgaaac acggcccgct ggcgctgatt
    19201 gacgcggata tgccggttat cgtcgttgca ccgaacaacg aactgttgga aaaactgaaa
    19261 tccaacattg aagaagttcg cgcgcgtggc ggtcaattgt atgtcttcgc cgatcaggat
    19321 gcgggttttg taagtagcga taacatgcac atcatcgaga tgccgcatgt ggaagaggtg
    19381 attgcaccga tcttctacac cgttccgctg cagctgctgg cttaccatgt cgcgctgatc
    19441 aaaggcaccg acgttgacca gccgcgtaac ctggcaaaat cggttacggt tgagtaatca
    19501 ctatcataga taaaaaaggg ctgctgtggg cggacaataa agtcttaaac tgaacaaaat
    19561 agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag
    19621 tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat
    19681 tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg catggtaaag
    19741 actatattcc atggctaaca gtacaagaag ttccttcttc aggtcgttcc caccgtattt
    19801 attctcataa gacgggacga gtccatcatt tgctatctga cttagagctt gctgtttttc
    19861 tcagtcttga gtgggagagc agcgtgctag ggaaggtgcg aacaagttcc tgatatgaga
    19921 tcatcatatt catccggagc gcatcccaga gggacatcat gagccatcaa ctcaccttcg
    19981 ccgatagtga attcagcact aagcgccgtc agacccgaaa agagattttc ctctcccgca
    20041 tggagcagat tctgccatgg cagaatatga ccgc
//
LOCUS       DAZXUL010000077        20065 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZXUL010000077 DAZXUL010000000
VERSION     DAZXUL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20065
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.077"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            285..1379
                     /gene="gcvT"
                     /locus_tag="ACH3L1_002727"
     CDS             285..1379
                     /gene="gcvT"
                     /locus_tag="ACH3L1_002727"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFT4077406.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1403..1792
                     /gene="gcvH"
                     /locus_tag="ACH3L1_002728"
     CDS             1403..1792
                     /gene="gcvH"
                     /locus_tag="ACH3L1_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFT4077407.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1911..4784
                     /gene="gcvP"
                     /locus_tag="ACH3L1_002729"
     CDS             1911..4784
                     /gene="gcvP"
                     /locus_tag="ACH3L1_002729"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFT4077408.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5051..5794
                     /locus_tag="ACH3L1_002730"
     CDS             5051..5794
                     /locus_tag="ACH3L1_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFT4077409.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5851..7284)
                     /gene="bglA"
                     /locus_tag="ACH3L1_002731"
     CDS             complement(5851..7284)
                     /gene="bglA"
                     /locus_tag="ACH3L1_002731"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFT4077410.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            7329..7639
                     /gene="yqfB"
                     /locus_tag="ACH3L1_002732"
                     /pseudo
     CDS             7329..7639
                     /gene="yqfB"
                     /locus_tag="ACH3L1_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            7803..8462
                     /locus_tag="ACH3L1_002733"
     CDS             7803..8462
                     /locus_tag="ACH3L1_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFT4077411.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8658..9638)
                     /gene="ygfZ"
                     /locus_tag="ACH3L1_002734"
     CDS             complement(8658..9638)
                     /gene="ygfZ"
                     /locus_tag="ACH3L1_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFT4077412.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9881..10147
                     /gene="sdhE"
                     /locus_tag="ACH3L1_002735"
     CDS             9881..10147
                     /gene="sdhE"
                     /locus_tag="ACH3L1_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFT4077413.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            10128..10535
                     /locus_tag="ACH3L1_002736"
     CDS             10128..10535
                     /locus_tag="ACH3L1_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFT4077414.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10575..11096)
                     /gene="fldB"
                     /locus_tag="ACH3L1_002737"
     CDS             complement(10575..11096)
                     /gene="fldB"
                     /locus_tag="ACH3L1_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFT4077415.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11208..12104
                     /gene="xerD"
                     /locus_tag="ACH3L1_002738"
     CDS             11208..12104
                     /gene="xerD"
                     /locus_tag="ACH3L1_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFT4077416.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12129..12839
                     /gene="dsbC"
                     /locus_tag="ACH3L1_002739"
     CDS             12129..12839
                     /gene="dsbC"
                     /locus_tag="ACH3L1_002739"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFT4077417.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            12845..14578
                     /gene="recJ"
                     /locus_tag="ACH3L1_002740"
     CDS             12845..14578
                     /gene="recJ"
                     /locus_tag="ACH3L1_002740"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFT4077418.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14669..15767
                     /gene="prfB"
                     /locus_tag="ACH3L1_002741"
     CDS             join(14669..14743,14745..15767)
                     /gene="prfB"
                     /locus_tag="ACH3L1_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFT4077419.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15777..17294
                     /gene="lysS"
                     /locus_tag="ACH3L1_002742"
     CDS             15777..17294
                     /gene="lysS"
                     /locus_tag="ACH3L1_002742"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFT4077420.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17337..17885)
                     /gene="idi"
                     /locus_tag="ACH3L1_002743"
     CDS             complement(17337..17885)
                     /gene="idi"
                     /locus_tag="ACH3L1_002743"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFT4077421.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            17940..18011
                     /locus_tag="ACH3L1_002744"
     CDS             17940..18011
                     /locus_tag="ACH3L1_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077422.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(18008..18133)
                     /gene="yqfG"
                     /locus_tag="ACH3L1_002745"
     CDS             complement(18008..18133)
                     /gene="yqfG"
                     /locus_tag="ACH3L1_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFT4077423.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18135..19583)
                     /gene="uacT"
                     /locus_tag="ACH3L1_002746"
     CDS             complement(18135..19583)
                     /gene="uacT"
                     /locus_tag="ACH3L1_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFT4077424.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         4948 a         5011 c         5376 g         4730 t
ORIGIN      
        1 agctcttaat attgtcttat tcaaattgac tttctcatca catcatcttt gtatagaaat
       61 tggtgtattt tttggttttt tattctgtcg cgatttttgc attttttaac cataagctaa
      121 tgtgatgatc aattttacct tatggttaac agtctgtttc ggtggtaagt tcaggcaaaa
      181 gagaacgatt gcgttgggga ccgggagtgg ctccgatgct gggttttgtg gtgataattt
      241 caccatgaaa aagttgtcag ccccgcttat tcaatgagga caagatggca caacagactc
      301 ctttgtacga acaacacacg ctttgcggcg ctcgcatggt ggatttccac ggctggatga
      361 tgccgctgca ttacggttcg caaatcgacg aacatcatgc ggtacgtacc gatgccggaa
      421 tgtttgatgt gtcgcatatg accatcgtcg atctccgcgg cagccgcacc cgggagtttc
      481 tgcgttatct gctggcgaac gatgtggcga agctcaccaa aagcggcaaa gccctttact
      541 cggggatgtt gaatgcctct ggcggtgtga tagatgatct catcgtctac tactttactg
      601 aagatttctt ccgcctcgtt gttaactccg ccacccgcga aaaagacctc tcctggatta
      661 cccaacacgc tgaacctttc ggcatcgaaa ttaccgttcg tgatgacctt tccatgattg
      721 ccgtgcaagg gccgaatgct caggcaaaag ctgccacact gtttaatgat gctcagcgtc
      781 aggcggtgga agggatgaaa ccgttctttg gcgtgcaggt gggcgatctg tttattgcca
      841 ccactggtta taccggtgaa gcgggctatg aaattgcgct gcccaatgaa aaagcggccg
      901 atttctggcg tgcgctggtg gaagcgggcg ttaagccctg cggtctgggg gcgcgtgaca
      961 cgctgcgtct ggaagcgggc atgaatcttt atggtcagga gatggacgaa actatttctc
     1021 ctttagccgc caacatgggc tggactatcg cctgggaacc gacagatcgt gactttatcg
     1081 gtcgtgaagc cctggaagtg cagcgtgagc atggtacaga aaaactggtt ggtctggtga
     1141 tgaccgaaaa aggcgtgctg cgtaatgaac tgccggtacg ctttaccgat gcgcagggca
     1201 accagcatga aggcattatc accagcggta ctttctcccc gacgctgggt tacagcattg
     1261 cgctggcgcg cgtgccggaa ggtattggcg aaacggcgat tgtgcaaatt cgcaaccgtg
     1321 aaatgccggt taaagtgaca aaacctgttt ttgtgcgtaa cggcaaagcc gtcgcgtgat
     1381 ttactttttt ggagattgat tgatgagcaa cgtaccagca gaactgaaat acagcaaaga
     1441 acacgaatgg ctgcgtaaag aagccgacgg cacttacacc gttggtatta ccgaacatgc
     1501 tcaggagctg ttaggcgata tggtgtttgt tgacctgccg gaagtgggcg caacggttag
     1561 cgcgggcgat gactgcgcgg ttgccgaatc ggtaaaagcg gcgtcagaca tttatgcgcc
     1621 agtaagcggt gaaatcgtgg cggtaaacga cgcactgagc gattccccgg aactggtgaa
     1681 cagcgaaccg tatgcaggcg gctggatctt taaaatcaaa gccagcgatg aaagcgaact
     1741 ggaatcactg ctggatgcga ccgcatacga agcattgtta gaagatgagt aacggcttta
     1801 ttcctcttct gcgggagagg atcagggtga ggaaaattta tgcctcaccc tcactctctt
     1861 cgtaaggaga gaggttcaca attcactgca cgtttcagga accatcgctc atgacacaga
     1921 cgttaagcca gcttgaaaac agcggcgctt ttattgaacg ccatatcgga ccggacgccg
     1981 cgcaacagca agaaatgctg aatgccgttg gtgcacaatc gttaaacgcg ctgaccggcc
     2041 agattgtgcc gaaagatatt cagcttgcga caccaccgca ggttggcgca ccggcgaccg
     2101 aatacgccgc actggcagaa ctcaaggcta ttgccagtcg caataaacgc ttcacgtctt
     2161 acatcggcat gggttacacc gccgtgcagc taccgccggt tatcctgcgt aacatgctgg
     2221 aaaatccggg ctggtatacc gcgtacactc cgtatcagcc tgaagtctcc cagggccgcc
     2281 ttgaagcact gctcaacttc cagcaggtaa cgctggattt gactggactg gatatggcct
     2341 ctgcttctct tctggacgag gccaccgctg ccgccgaagc aatggcgatg gcgaaacgcg
     2401 tcagcaaact gaaaaatgcc aaccgcttct tcgtggcttc caatgtgcat ccgcaaacgc
     2461 tggatgtggt ccgtactcgt gctgaaacct ttggttttga agtgattgtc gatgacgcgc
     2521 aaaaagtgct cgaccatcag gacgtcttcg gcgtgctgtt acagcaggta ggcactaccg
     2581 gtgaaattca cgactacact gcgcttatta gcgaactgaa atcacgcaaa attgtggtca
     2641 gcgttgccgc cgatattatg gcgctggtgc tgttaactgc gccgggtaaa cagggcgcgg
     2701 atattgtttt tggttcggcg caacgcttcg gcgtgccgat gggctacggt ggcccacacg
     2761 cggcattctt tgcggcgaaa gatgaataca aacgctcaat gccgggccgt attatcggtg
     2821 tatcgaaaga tgcagctggc aataccgcgc tgcgcatggc gatgcagact cgcgagcaac
     2881 atattcgtcg tgagaaagcg aactccaaca tttgtacttc ccaggtactg ctggcaaaca
     2941 tcgccagcct gtatgccgtt tatcacggcc cggttggcct gaaacgtatc gctaaccgca
     3001 ttcaccgtct gaccgatatt ctggcggcgg gcctgcaaca aaaaggtctg aaactgcgcc
     3061 atgcgcacta tttcgacacc ttgtgtgtgg aagtggccga caaagcgggc gtactgacgc
     3121 gtgccgaagc ggctgaaatc aacctgcgta gcgatatcct gaacgccgtg gggatcaccc
     3181 ttgatgaaac caccacgcgt gaaaacgtaa tgcagctttt cagtgtgctg ctgggcgata
     3241 accacgggct ggagatcgac acgctggaca aagacgtggc tcacgacagc cgctctatcc
     3301 agcctgcgat gctgcgcgac gacgaaatcc tcacccatcc ggtgtttaat cgctatcaca
     3361 gcgaaaccga aatgatgcgc tatatgcact cgctggagcg taaagatctg gcgctgaatc
     3421 aggcgatgat cccgctgggt tcctgcacca tgaaactgaa cgccgccgcc gagatgatcc
     3481 caatcacttg gacggaattt gccgaactgc acccgttttg cccgccggaa caggctgaag
     3541 gttatcagca gatgattgcg cagctggctg actggctggt gaaactgacc ggttacgacg
     3601 ccgtttgtat gcagccgaac tccggcgcac agggcgaata cgcgggcctg ctggcgattc
     3661 gtcattatca tgaaagccgc aacgaagggc atcgcgatat ctgcctgatc ccggcttctg
     3721 cgcacggaac taaccccgct tctgcacata tggcaggaat gcaggtggtg gttgtggcgt
     3781 gtgataaaaa cggcaacatc gatctgactg atctgcgcgc gaaagcggaa caggcgggcg
     3841 ataacctctc ctgtatcatg gtgacttatc cttctaccca cggcgtgtat gaagaaacga
     3901 tccgtgaagt gtgtgaagtc gtgcatcagt tcggcggtca ggtttacctt gatggcgcga
     3961 acatgaacgc ccaggttggc atcacctcgc cgggctttat tggcgcggac gtttcgcacc
     4021 tcaacctgca taaaactttc tgcattccgc acggcggtgg tggtccgggt atgggaccga
     4081 tcggtgtgaa agcgcatctg gcaccgtttg taccgggtca tagcgtggtg caaatcgaag
     4141 gcatgttaac ccgtcagggc gcagtttctg cggcaccgtt cggtagcgcc tctatcctgc
     4201 caatcagctg gatgtacatc cgcatgatgg gcgcagaagg gctgaaaaaa gcaagccagg
     4261 tggcaatcct caacgccaac tatattgcca gccgcctgca ggatgccttc ccggtgctgt
     4321 ataccggtcg cgacggtcgc gtggcgcacg aatgtattct cgatattcgc ccgctgaaag
     4381 aagaaaccgg catcagcgag ctggacattg ccaagcgcct gatcgactac ggtttccacg
     4441 cgccgacgat gtcgttcccg gtggcgggta cgctgatggt tgaaccgact gaatctgaaa
     4501 gcaaagtgga actggatcgc tttatcgacg cgatgctggc tatccgcgca gaaattgacc
     4561 aggtgaaagc cggtgtctgg ccgctggaag ataacccgct ggtgaacgcg ccgcacattc
     4621 agagcgaact ggtcgccgag tgggcgcatc cgtacagccg tgaagttgcg gtattcccgg
     4681 caggcgtggc agacaaatac tggccgacgg tgaaacgtct ggatgatgtt tacggcgacc
     4741 gtaacctgtt ctgctcctgc gtaccgatta gcgaatacca gtaattcact gattcgagta
     4801 ttttctaaag gcgcttcggc gcctttttag tcagatgaca aagtacaaaa gtgctcagac
     4861 agtcccctcg cccctttggg gagagggtta gggtgagggg aacaggccgg cactggcgcg
     4921 aatatttacc ctcaccccga ccctctccct gaaagggcga gggggaaaag cgtgccaaca
     4981 ttgaagattg agccagtttg ttagcaatct caaagatacg tcaacgaatt aatttttctc
     5041 ggaaaaacaa atggctatag cacttgtgac cggtggcagt cgcggcatcg ggcgggcaac
     5101 tgcattactg ttggcgcaag aagggtatac ggtggcggtt aattatcagc aaaacctcca
     5161 cgcggcgcag gaagtgatga acttaataat gcaagccggt ggcaaagcat tcgtgctcca
     5221 ggcggatatc agcgacgaaa accaggtcgt tgcgatgttt acagcaatcg atcagcacga
     5281 tgaaccgcta gcagcgctgg tcaataacgc cgggatcttg tttacccagt gcaccgttga
     5341 aaaccttacc gcagagcgaa tcaaccgagt actttccacc aacgtgacgg gatattttct
     5401 ctgctgccgc gaggcggtaa aacgcatggc gcttaaaaat ggtggcagtg gcggcgctat
     5461 cgtcaatgtc tcttcggtgg cctcacggtt gggttcgcca ggggaatatg ttgattacgc
     5521 ggcatcgaaa ggggcgattg attcgttaac caccggacta tcgctggaag tcgccgcgca
     5581 ggggatccgc gttaactgcg tgcggccagg gtttatttat accgaaatgc acgccagcgg
     5641 cggcgagcct ggacgcgtcg atcgcgttaa gtcgaacatc cccatgcagc gtggtggaca
     5701 ggcagaagag gtcgcgcagg ccattgtctg gctactaagt gataaagcct cttacgtcac
     5761 gggaagtttt atcgatttgg cgggcgggaa ataaaacagg gaagttgtct gaccggatgc
     5821 aacaagtatt gcatccggta cttcatcgac ttaaagcttc tcgccgttgc tggcaatcac
     5881 ctctttgtac cagttaaagc tcttcttacg tgaacgcgac atatcgccag taccgtcgtc
     5941 atgtttattc acatagataa agccgtagcg tttgctgtac tgcccggtag tgaatgacac
     6001 gcagtcgatg cagccccacg gtgtgtagcc catcagatcc acaccatcgt aagtcactgc
     6061 ttttttcatc tcttcaatat gggcgcgcag gtagtcaatg cggtagtcgt cgttgatgct
     6121 gccatcttct tccactttgt cgtaagcgcc aaaaccgttt tcgacaataa acagcggcct
     6181 ctgataacgc tcatacagtt cgcaaagggc atagcgcaga cctactggat caatctgcca
     6241 gccccagtca gacgctttaa catacgggtt cggtacgctg ccttcaaaac cagagatcgc
     6301 atcgccggtg ccgccttcgg ccttcactgc gttggtcatg taatagctga aaccaagata
     6361 atcgcaggtg ccttcgcgca gtacatcgag atcgccgtct tccattttga tattaaatcc
     6421 gcgacgttcc cactcgttca atacatagga cgggtaatag ccgcatagct gcacatcggt
     6481 aaagacgtag cgttcgcgca tcgactcctg agcgaacatc acatcgtccg ggttacatga
     6541 gtagggatag agcggcacca tcgccagcat acagccgact ttcatctccg ggttaatgcg
     6601 acgcgcggct ttcactgcca gggcgctggc gacaaactgg tgatgcagca cctggtacat
     6661 cgtctcttcc gggttgtcat gctcggtata caccacgccg gagcagcagt aaccaaacag
     6721 cggcgcacgc cagttacgct ggttgtttat ttcgttgaag gtcatccagt atttgacttt
     6781 atgcttgtag cgttcgaata ccacttccgc gaaacggaca aagaaatcaa cgaatttacg
     6841 gttggtccag ctaccgtatt gctgcaccag atgcagcggc atttcaaagt gggagagggt
     6901 tatcaccggt tcgatgttgt atttcagcag ttcatcgaac agagagtcgt aaaacttcag
     6961 cccttcttcg tttggctgag cttcatcacc ttttggaaaa atacgcgtcc aggcgatgga
     7021 agtccgaaaa catttgaagc ccatttcggc aaatagcttg atgtcttcct tgtagtgacc
     7081 ataaaaatca acggcttcat ggtttggata gtattttcct ggcaagactt ctttggtgat
     7141 ttcgcgcggc acgccgtgtg cgccacaggt cagaacgtca caaatgctcg gtcctttgcc
     7201 gcctttgttc cagccgcctt cgacctgatg agcggcaacg gcgccacccc ataagaaatc
     7261 tttcggtaag gtgagttttt tcactatcat aggctccaga tcagtcaatt cttacaggaa
     7321 atagtaccat gcagccaaac gacatcactt ttttcaacgt ttccaggatg acattctggc
     7381 tgggcgtaaa accatcacca tccgcgacga gtctgaatcg cacttcaaaa cgggtgatgt
     7441 gcttcgtgtc ggacgttttg aagatgacgg ttatttttgc acgattgaag tcaccgcaac
     7501 ctcaaccgta acgctggata cactgacgga aaaacatgca gagcaggaaa atatgaccct
     7561 gcctgaactg aaaaaggtca ttgctgacat ctatcccggt cagacacaat tttatatgat
     7621 tgaatttaaa tgtctttaaa ttgaactcac gcctgttagt tgaaatttcg catttaattt
     7681 catgattttt aggagattag tctcaaatca gaatttattt tagctaacag gtgttcactg
     7741 gaactattct cagttacgct ggagaggtat acatgtgcgt gtacgtttct ctggagtaag
     7801 ttatggttca gaagcccctc attaagcagg gatattcgct ggcagaggaa attgccaaca
     7861 gcgtcagtca cggcattggg ctggtgtttg gtatcgttgg gctggtgttg ctactggttc
     7921 aggcggtgga tctcaatgcc agcgccacag cgataaccag ttacagcctc tatggcggca
     7981 gtatgatcct gctgttcctc gcttcgacgc tctatcacgc cattcctcat caacgggcaa
     8041 aaatgtggct gaagaaattt gaccattgcg ctatttacct gttgattgcc ggaacctaca
     8101 cgccgttttt gctggtgggg ctggattctc cgttagcgcg cgggttgatg attgttatct
     8161 ggagcctagc attgctgggg attctgttca aactgaccat cgcgcaccga ttcaaaattt
     8221 tatctctggt gacttatctg gcgatgggct ggctgtcgct ggtggtaatt tatgaaatgg
     8281 cagttaagct cgcggcgggc agcgttacct tactggcggt aggcggcgtg gtttattcgc
     8341 tcggggtgat tttctacgtc tgcaaacgca ttccatacaa ccatgccatc tggcacggct
     8401 tcgtgctcgg cggtagtgtg tgccactttc tggcgatcta tttgtatatt gggcaggcgt
     8461 aatgggtatg cctttgtagg cctgataaga cgcgtcagcg tcgcatcagg catcggagcg
     8521 caaccgccgg atgcggcgtg aacgccttat ccgatctacg ggcctcgtcc ctgttgtgat
     8581 aaaggcatca aatcccagac atgataagac gcgtcagcgt cgcatcaggc gataaatgcc
     8641 tgatgcgata cagagattta ctcttcgagc gaatacggca gcggctcgat atgcagtgta
     8701 ttcgcatcgt cgcgtacgcg gaagatgctg tccggttcca tatcgttatt catgacgacc
     8761 tgtaccacga cctgaccatc ttccagtttt accgcagcca gcaccgtacc ggtacgacgc
     8821 cagttctcgc ccattttcag ctctaagtct tcaccagctt ccggcagtcg gctggcacta
     8881 cctgccagca accagagcgc gcgtttgttg gcaccacgga attttgctcg cgccaccatc
     8941 tcttgtccgg tataacagcc tttcttaaag ctgataccgc ccagcgcctg gagattggtc
     9001 gcctgtggga taaactgccc gctgttggcg gcatcaatca ccgggaaacc agcttcaatg
     9061 tttaatgcca gccactgttg gctattgttc agttccgctt caccgcgcag tttatcggtc
     9121 agcatattag cagtagcttc atcggttacg atcaggaaac gttctgccgg gtgttcaaac
     9181 catagcaaag tggtcgcgcc ttctttgact acctgttttt ctttcgaagg cagttcgcta
     9241 aagagatttg ccagcgcggc gcgcgcctga aaaccggcaa caccaagcag cacacgctcg
     9301 tcgtctggcg cgatggtcac tttagagaat accgcatatt ttttcagttc agtcagctgc
     9361 ggttcacgca cgctgcgccg ttcaatccat gcaaagccat cgccgtcgcg gaacagacgt
     9421 aaattgctcc acattttacc tttggcgtcg caatgggcgg cgagcaggtg ctgatcttct
     9481 gccatctggc tgacatctgc tgtcacctga ccctgcatat atttttcgct gtccgcgcca
     9541 gtaatggtgg caagcgccca gtcatcaagc gtcatcagcg tcagcggtaa acgggcagaa
     9601 gccgtaggct gacgtggagg aaaaggtgta aaagccataa tagagtcctg agttgcttaa
     9661 cggcgtatta atgactaatg gtaaaagagc tatgaggcaa tgcaagtgct ttcactacgc
     9721 gatctgtgcc ttctgcgagg cgactgccaa aaaaatgaag cccttgaatt gttgtgaatt
     9781 aatttcgcga tcgcgatggt gtttactgat attcccgtaa aagaagtcga aaaacacgtt
     9841 acaatagccg ggtactggat tttcgtgaga caggaagaag atggacatta acaacaaagc
     9901 ccgaattcat tgggcatgcc gccgtggtat gcgcgaactc gatatttcaa tcatgtcgtt
     9961 tttcgaacat gagtacgaca gcttaagcga tgacgaaaaa cgcatcttta ttcgtctgct
    10021 ggaatgtgac gatccggacc tgtttaactg gctgatgaat cacggtaaac cggccgatgc
    10081 agaactggaa ataatggtcc gactcatcca gacacggaac cgggaacgtg gtcctgtggc
    10141 aatctgattt gcgcgtctcc tggcgcgcac agtggctttc cttgctgatt catgggctgg
    10201 ttgccgctgt tattttactc atgccctggc cgctcagtta caccccgtta tggatggtgt
    10261 tactttcgct ggtggtattt gattgcgttc gcagccagcg gtgtattaat gctcgccagg
    10321 gggaaattcg cttgttgatg gacgggcgtt tgcgttggca agggcaggag tggagcatcg
    10381 tcaaagcacc gtggatgatt aagagcggca tgatgctgcg tttacgttct gatggcggta
    10441 aacggcaaca tttatggctg gcagccgaca gcatggacga agccgaatgg cgggatttac
    10501 ggcggatttt gttgcaacaa gagacgcaaa gataaagatg acggcaaagc atatgcgcta
    10561 agccgtcagt gagatcaggc gtaatgctct gccatttcgt tgaggatttg ctcgcaccag
    10621 ctctgaatac gctcgtcgct aaggtcatac tggttagttt catccagcgc cagacccacg
    10681 aacagttgcc cgtcagcaat caccggtttc gggctggtaa attcatatcc ttccgttggc
    10741 cagtagccga cgaacttcac gcctttggtc gagagtttgt catgcagcat accgagcgca
    10801 tcgaggaacc actcgccgta tcccagttga tcgccaagcc catacagcgc aataatttta
    10861 ccttcaaggt tcaggtcgtc gagctgatcc cagacggctt cccagtcttc ctggatttca
    10921 ccaaaatccc aggtcgggat acccagaatg agcacatcgt actgctccat taatttcggg
    10981 gagtcgtcct tgaggttatg taaggtcacc agttctgggc cgataatatc gcggattttt
    11041 tctgccgcca tttcggtgta acaggtgctg gaaccgtaaa aaagacccat attcataacg
    11101 taagcttctc gattctgctg tggcttttgc agctagtgta tcagaatcgc tttagtcacg
    11161 gcacaataag gcataatgga tcaaatgagt gaccataagg ggcgcaagtg aaacaggagc
    11221 tggcacgcat cgagcagttt cttgatgctc tgtggctgga aaaaaatctg gctgaaaata
    11281 cgttgaacgc ttaccgtcgc gatctgtcaa tgatggtgga gtggttgcat caccgcgggt
    11341 tgacgctggc gacggcgcaa agtgacgatt tgcaggcatt actggcagaa cggctggagg
    11401 gcgggtataa agccaccagc tcagcgcgtt tgctgagtgc agtgcgccga ttgttccagt
    11461 atctttatcg cgaaaagttt cgtgaagacg atcccagtgc gcatctcgct tcaccgaaat
    11521 tgccccagcg tttgccaaaa gatttaagcg aagcgcaggt cgaacgttta ttacaggcgc
    11581 cattaattga tcagccactg gagctacgcg ataaagccat gcttgaagtg ttgtatgcta
    11641 ccgggctgcg tgtctctgaa ctggtcgggc tgacaatgag tgatatcagc ctgcgtcagg
    11701 gcgtagtgcg ggtcattggt aaaggcaaca aagagcgcct ggtgccgtta ggggaagagg
    11761 cggtttactg gctggaaacc tatctggaac atgggcgtcc gtggctgctg aatggtgtgt
    11821 caattgacgt gttgtttccc agccagcgtg cgcagcagat gacgcgacag accttctggc
    11881 accgcattaa acattatgcc gtgctggcgg gtatcgacag cgaaaagctg tcaccgcatg
    11941 tgttgcgtca cgcttttgcc actcatttat taaatcatgg tgcggattta cgcgtggtgc
    12001 agatgctact gggccacagc gatctttcca ccacgcaaat ttatacgcat gtcgctaccg
    12061 agcgtctgcg gcaacttcat caacagcatc acccgcgggc gtgatgtctg aaaagaacgg
    12121 gaagatttat gaagaaaggt tttatgttgt ttactttgtt agcggcgttt tcaggctttg
    12181 ctcaggctga tgacgcggca attcaacaaa cgttagccaa aatgggcatc aaaagcagcg
    12241 atattcagcc cgcgcccgta gctggcatga agacagttct gactaacagc ggtgtgttgt
    12301 acatcaccga tgatggtaaa catatcattc aggggccaat gtatgacgtt agtggcacgg
    12361 ctccggtcaa tgtcaccaat aagatgctgt taaagcagtt gaatgcgctt gaaaaagaga
    12421 tgatcgttta taaagcgccg caggaaaaac acgtcatcac cgtgtttact gatattacct
    12481 gtggttactg ccacaaactg catgagcaaa tggcagacta taacgcgctg gggatcaccg
    12541 tgcgttatct tgctttcccg cgccaggggc tggacagcga tgcagagaaa gaaatgaaag
    12601 ctatctggtg tgcgaaagat aaaaacaaag cgtttgatga tgtgatggca ggtaaaagcg
    12661 tcgcaccagc cagctgcgac gtggatattg ccgaccatta cgcacttggc gtccagcttg
    12721 gcgttagcgg tactccggca gttgtgctga gcaatggcac acttgttccg ggttaccagc
    12781 cgccgaaaga catgaaagaa ttcctcgacg aacaccaaaa aatgaccagc ggtaaataat
    12841 tcgcgtgaaa caacagatac aacttcgtcg ccgtgaagtc gatgaaacgg cagacttgcc
    12901 cgctgaattg cctcccttgc tgcgccgttt atacgccagc cggggagtac gcagtgcgca
    12961 agaactggaa cgcagtgtta aaggtatgtt gccctggcag caactaagcg gtgtcgaaaa
    13021 ggccgttgag atcctttaca acgcttttcg cgaaggaacg cggattattg tggtcggtga
    13081 tttcgacgcc gacggcgcga ccagcacggc tctaagcgtg ctggcgatgc gctcgcttgg
    13141 ttgcagcaat atcgactacc tggtaccaaa ccgtttcgaa gacggttacg gcttaagccc
    13201 ggaagtggtc gatcaggccc atgcccgtgg cgcgcagtta attgtcacgg tggataacgg
    13261 tatttcctcc catgcgggag ttgagcacgc tcgctcgttg ggcatcccgg ttattgttac
    13321 cgatcaccat ttgccgggcg acacattacc cgcagcggaa gcgatcatta accctaactt
    13381 gcgcgactgt aatttcccgt cgaaatcact ggcaggcgtg ggtgtggcgt tttatctgat
    13441 gctggcgctg cgcacctttt tgcgcgatca gggctggttt gatgagcgcg gcatcgcaat
    13501 tcctaacctg gcagaactgc tggatctggt agcactgggg acagtagcgg acgtcgtgcc
    13561 gctggacgct aataatcgca ttctgacctg gcaggggatg agtcgcatcc gtgccggaaa
    13621 gtgccgtcca gggattaaag cgctgctgga agtggcaaat cgcgatgcac aaaaactagc
    13681 cgccagcgat ttaggttttg cgctgggacc gcgtctcaat gctgccgggc gactggatga
    13741 tatgtccgtt ggtgtggcgc tgttgctgtg cgacaacatc ggcgaagcgc gcgtgctggc
    13801 aaatgaactc gatgcgctaa accagacgcg aaaagagatc gaacaaggaa tgcaggttga
    13861 agccctgacc ctgtgcgaga aactggagcg cagccgtgac acgctacccg gcgggctggc
    13921 aatgtatcac cccgaatggc atcagggcgt tgtcggtatt ctggcttcgc gtatcaaaga
    13981 gcgttttcac cgtccggtta tcgcctttgc gccagcaggt gacggtacgc tgaaaggttc
    14041 cggtcgctcc attcaggggc tgcatatgcg tgatgcgctg gagcgattag acacactcta
    14101 ccccggcatg atgctcaagt ttggcggtca tgcgatggcg gcgggtttgt cgctggaaga
    14161 ggataaattc gaactctttc aacaacggtt tggcgaactg gttactgagt ggctggcccc
    14221 ttcgctattg caaggcgaag tggtgtcaga cggtccgtta agcccggccg aaatgaccat
    14281 ggaagtggcg cagctgctgc gcgatgctgg cccgtggggg cagatgttcc cggagccgct
    14341 gtttgacggt catttccgtc tgctgcaaca gcggctggtg ggcgaacgtc atttgaaggt
    14401 gatggtcgaa ccggtcggcg gcggtccact gctggatggt attgctttta atgtcgatac
    14461 cgctctctgg ccggataacg gcgtgcgcga agtgcaactg gcttataagc tcgatatcaa
    14521 cgagtttcgc ggcaaccgca gcctgcaaat tatcatcgac aatatctggc caatttagcg
    14581 tcatcttctc tataaaaaag agcgtggatt gggtacaatc ccgctcttat caccgcattt
    14641 tgactagctc aataaaagaa atcagaccat gtttgaaatt aatccggtaa ataatcgcat
    14701 tcaggacctc acggaacgct ccgacgttct tagggggtat ctttgactac gacgccaaga
    14761 aagagcgtct ggaagaagta aacgccgagc tggaacagcc ggatgtctgg aacgaacccg
    14821 aacgcgcaca ggcgctgggt aaagagcgtt cctccctcga agccgttgtc gacaccctcg
    14881 accaaatgaa acaggggctg gaagatgttt ctggtctgct ggaactggct gtagaagctg
    14941 acgacgaaga aacctttaac gaagccgttg ctgaactcga cgccctggaa gaaaaactgg
    15001 cgcagcttga gttccgccgt atgttctctg gcgaatatga cagcgccgac tgttacctcg
    15061 atattcaggc ggggtctggc ggtacggaag cacaggactg ggcgagcatg cttgagcgta
    15121 tgtatctgcg ctgggcagaa tcgcgtggtt tcaaaactga aatcatcgaa gagtcggaag
    15181 gtgaagtggc gggtattaaa tccgtgacga tcaaaatctc cggcgattac gcttacggct
    15241 ggctgcgtac agaaactggc gttcaccgcc tggtgcgtaa aagcccgttt gactccggcg
    15301 gtcgtcgcca cacgtcgttc agctctgcgt ttgtttatcc ggaagttgat gatgatattg
    15361 atatcgaaat caacccggcg gatctgcgca ttgacgttta tcgcgcgtcc ggcgcgggcg
    15421 gtcagcacgt taaccgtacc gaatctgcgg tgcgtattac ccacatcccg accgggatcg
    15481 tgacccagtg ccagaacgac cgttcccagc acaagaacaa agatcaggcc atgaagcaga
    15541 tgaaagcgaa gctttatgaa ctggagatgc agaagaaaaa tgccgagaaa caggcgatgg
    15601 aagataacaa atctgacatc ggctggggca gccagattcg ttcttatgtc cttgatgact
    15661 cccgcattaa agatctgcgc accggggtag aaacccgcaa cacgcaggcc gtgctggacg
    15721 gcagcctgga tcaatttatc gaagcaagtt tgaaagcagg gttatgagga accaacatgt
    15781 ctgaacaaca cgcacagggc gctgacgcgg tagtcgatct taacaatgaa ctgaaaacgc
    15841 gtcgtgagaa gctggcgaac ctgcgtgagc agggaattgc cttcccgaac gatttccgtc
    15901 gcgatcatac ctctgaccaa ttgcacgcag aattcgacgg taaagagaac gaagaactgg
    15961 aagcgctgaa catcgaagtc gccgttgctg gccgcatgat gacccgccgt attatgggta
    16021 aagcgtcttt cgttaccctg caggatgtgg gtggccgcat tcagctgtac gttgcccgtg
    16081 acgatctgcc ggaaggcgtt tacaacgagc agttcaaaaa atgggacctc ggcgacatcc
    16141 tcggcgcgaa aggtaaactg ttcaaaacca aaaccggcga actgtctatc cactgtaccg
    16201 agctgcgtct gctgaccaaa gcactgcgtc cgctgccgga taaattccac ggcttgcagg
    16261 atcaggaagc gcgctatcgt cagcgttatc tggatctcat ctctaacgat gaatcccgta
    16321 acacctttaa agtacgctcg cagatcctct ctggtattcg ccagttcatg gtgaaccgcg
    16381 gctttatgga agttgaaacg ccgatgatgc aggtgatccc aggcggtgcc gctgcgcgtc
    16441 cgtttatcac ccaccataac gcgctggatc tcgacatgta cctgcgtatc gcgccggaac
    16501 tgtacctcaa gcgtctggtg gtcggtggct tcgagcgtgt attcgaaatc aaccgtaact
    16561 tccgtaacga aggtatttcc gtacgtcata acccagagtt caccatgatg gaactctaca
    16621 tggcttacgc agattacaaa gatctgatcg agctgaccga atcgctgttc cgtactctgg
    16681 cacaggatat tctcggtaag acggaagtga cctacggcga cgtgacgctg gacttcggta
    16741 aaccgttcga aaaactgacc atgcgtgaag cgatcaagaa atatcgcccg gaaaccgaca
    16801 tggcggatct ggataacttc gactctgcga aagcgattgc tgaatctatc ggtatccacg
    16861 ttgagaagag ctggggtctg ggccgtatcg ttaccgagat cttcgaagaa gtggcagaag
    16921 cacatctgat tcagccgacc ttcattactg aatatccggc agaagtttct ccgctggcgc
    16981 gtcgtaacga cattaacccg gaaatcacag accgctttga gttcttcatc ggtggtcgtg
    17041 aaatcggtaa cggctttagc gagctgaatg acgcggaaga tcaggcgcaa cgcttcctgg
    17101 atcaggttgc cgcgaaagac gcaggtgacg acgaagcgat gttctacgat gaagattacg
    17161 ttaccgcact ggaacatggc ttaccgccga cagcaggtct ggggattggc atcgaccgta
    17221 tggtaatgct gttcaccaac agccatacca tccgcgacgt tattctgttc ccggcgatgc
    17281 gtccggtgaa ataagcatta cgttatgctc acaaccccgg caaatgtcgg ggttttttat
    17341 ttaagctggg taaatgcaga taatcgtatt ctggcttcgc gatttgtcgc ctgcatcacc
    17401 atccacggac tgaacgccca cggcgtggca tcaataccgc gtaatacatc agctaaatca
    17461 caccattgat aatccatcac ttcatcatcg ttgatctgta acgcactggt ggtgcgtgcg
    17521 gcaaataccg gacacacttc attttccaca atgcctctcg gatcggtggc gcggtagcga
    17581 aagtcaggat agatagattc aggaggcgta atttccacgc caagctcata acggcaacgg
    17641 cggatcaccg cgtcttcgct gctttctccc agttgtgggt gcccacaaac cgagttagtc
    17701 cacacgccag gccacgcttt tttgctcagt gcgcggcggg taactaataa ttgccccttg
    17761 gcattaaaca gccaactgga gaacgcgaga tgtaagaggg tgtctgccgt gtgtgcggca
    17821 tacttttcca gcgtacccgt gggaactccc tgtgcattca ataaaatgac gtgttccgtt
    17881 tgcataattt ctcacatgta attctgatca ctcatcatta tagataattg aagagtgaaa
    17941 tgattaacca agagagcgtt tatcgacaac cgcccgtttt gagcggatgc cgacaggtaa
    18001 aaaccattta aaaaagtagc atcatatatt tgaagcgact ttcaatggcc attgcaacac
    18061 agtcagaaat gacaggaata gagagagtaa aaagcaacaa cagactgaat atagcgcgca
    18121 ttaaaaaatt catattattc tccatgctca tttttcttca gcttgcgcat cagaagccac
    18181 atacgtactg tcctgacttc taaatcttta tcagagacgg attccttcac caggtcagca
    18241 tgatgctgat agccattaaa gaagacgttc agcacaacgg cgctaagggt tgcgagcata
    18301 atgccgctat gtagcagcgg ttgcagtacg gccggtaact tagaaaagaa atcgtgagag
    18361 agcgtcggag tcatgccaac gccgagactg atcgccacaa tatagaggtt gtaacggttg
    18421 gtggtgtagt tacagcgcga cagaattcga atccctgtcg ccagtaccat gccgaacatc
    18481 actagaccag cgccgcccag cacaaattgc ggaatggagg ctaccagcac cgccattttt
    18541 ggcaccatgc caaacaggat taatataatt cccgaagaaa tacacaccca gcggctatga
    18601 acgcgcatca cgctaaccag gccaacgttt tgagaaaatg acgtgtgggg gaagctgtta
    18661 aacgtgccgc ctatcattgt ccctacgcca tcgacacgca gcccgcaaat aatatcgtgt
    18721 gaagagagtt tacgaccgac tatttcaccc agtgccagga acatccccat cgactcgata
    18781 aacacgatga ttaacacggc agtcatggtc agaatggaaa cgggatcgaa aatcggcata
    18841 ccaaacgaca tcggggtgac aatcgcaaac cacgaggcat catgtagccc ggataaattg
    18901 acttcattca tcatccacga aagtaaaaag ccaaatacaa tccccagtaa tacggcgacg
    18961 ttggacataa atcctttcgc atagcgagta atgagcaaga taaaaattaa gacggcaaag
    19021 gagataccta aataaacggg attaccatat tgcggattcc ctttacctcc agcggcccag
    19081 tcaataccca cctgaatgat gctaagccca atagaagtaa taaccacacc ggtaaccagt
    19141 ggcgggaata aaggcatcaa gcgaccaata agtggcgcta ataatgtggt gataaaaccc
    19201 gcggcgatag tggcaccaaa tatccccagc aggccgatat ccgggttcat cccaatggct
    19261 atcattggtg ttacagcagc aaaggtcacc gacataatca ccggcaggcg gatccccata
    19321 aagcggccga taccgataca ttgcaataat gtgacgatcc cgcagcaaaa gagatccgag
    19381 ctaatgagca tcgcaatagc atcttttgag aggcccagtc ggtcaccaat cataagagga
    19441 acagcgactg cacctgcgta catgacaagg acgtgttgca gaccgagtat gatcagcttt
    19501 cctggtgata atatgcggtc aacctcatca gttgggcggt cttgcccaga agatgaggga
    19561 agttgggaat ctatggcgct catcagtgtt tctcctttcg cccgccagaa taaaaagcaa
    19621 ccgtttacgt taatgaaact gtctaaacac atgctcgtgg agtgccagtt tttgcgagga
    19681 aggggagtta gagacaggaa ttgcggaggc aatcacataa tcgtgcttaa accacgaaaa
    19741 tgacaagaga aactgacaat ggaattatct gtaattttct catattgata tttcattaat
    19801 cattctggca tgtcgcactc tcgcatttaa tcgtttttat ctggatagcg ctcttttgat
    19861 cggcaatgtt gaaataatga aagcggcata gattgagggg caataatttg aactcagtcg
    19921 caaaattata attaaaacgg tcatgctacc taatatatgc ccgtttattg catcgcgaaa
    19981 tttgagaaga cataactcat taaatgaaaa ggatgcaaat gaatacgttt atcgctgctg
    20041 aagctgcgga atgtataggc tgtca
//
LOCUS       DAZXUL010000078        19848 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZXUL010000078 DAZXUL010000000
VERSION     DAZXUL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19848
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.078"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..169
                     /locus_tag="ACH3L1_002747"
     CDS             <1..169
                     /locus_tag="ACH3L1_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HFT4077425.1"
                     /translation="EQIPTGSPTSYVLYLLVLVALFLLIPILIYAFRKSDWKDPNSDF
                     EPFSWEKRTSN"
     gene            203..1012
                     /gene="murI"
                     /locus_tag="ACH3L1_002748"
     CDS             203..1012
                     /gene="murI"
                     /locus_tag="ACH3L1_002748"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFT4077426.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(1131..1825)
                     /locus_tag="ACH3L1_002749"
     CDS             complement(join(1131..1579,1579..1825))
                     /locus_tag="ACH3L1_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFT4077427.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(2344..3717)
                     /gene="eutA"
                     /locus_tag="ACH3L1_002750"
     CDS             complement(2344..3717)
                     /gene="eutA"
                     /locus_tag="ACH3L1_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFT4077428.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(3692..4171)
                     /gene="eutP"
                     /locus_tag="ACH3L1_002751"
     CDS             complement(3692..4171)
                     /gene="eutP"
                     /locus_tag="ACH3L1_002751"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFT4077429.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(4184..4519)
                     /gene="eutS"
                     /locus_tag="ACH3L1_002752"
     CDS             complement(4184..4519)
                     /gene="eutS"
                     /locus_tag="ACH3L1_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFT4077430.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(4812..7091)
                     /gene="maeB"
                     /locus_tag="ACH3L1_002753"
     CDS             complement(4812..7091)
                     /gene="maeB"
                     /locus_tag="ACH3L1_002753"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFT4077431.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            7380..8330
                     /gene="tal"
                     /locus_tag="ACH3L1_002754"
     CDS             7380..8330
                     /gene="tal"
                     /locus_tag="ACH3L1_002754"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFT4077432.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            8350..10353
                     /gene="tkt"
                     /locus_tag="ACH3L1_002755"
     CDS             8350..10353
                     /gene="tkt"
                     /locus_tag="ACH3L1_002755"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFT4077433.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(10456..11499)
                     /locus_tag="ACH3L1_002756"
     CDS             complement(10456..11499)
                     /locus_tag="ACH3L1_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFT4077434.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(11625..12200)
                     /gene="nudK"
                     /locus_tag="ACH3L1_002757"
     CDS             complement(11625..12200)
                     /gene="nudK"
                     /locus_tag="ACH3L1_002757"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFT4077435.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(12268..14247)
                     /gene="aegA"
                     /locus_tag="ACH3L1_002758"
     CDS             complement(12268..14247)
                     /gene="aegA"
                     /locus_tag="ACH3L1_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFT4077436.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            14453..16170
                     /gene="narQ"
                     /locus_tag="ACH3L1_002759"
                     /pseudo
     CDS             14453..16170
                     /gene="narQ"
                     /locus_tag="ACH3L1_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            16372..19485
                     /gene="acrD"
                     /locus_tag="ACH3L1_002760"
     CDS             16372..19485
                     /gene="acrD"
                     /locus_tag="ACH3L1_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFT4077437.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(19584..19643)
                     /gene="ypfM"
                     /locus_tag="ACH3L1_002761"
     CDS             complement(19584..19643)
                     /gene="ypfM"
                     /locus_tag="ACH3L1_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFT4077438.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         4819 a         5162 c         4997 g         4870 t
ORIGIN      
        1 cgagcaaatc ccaactggta gtccaaccag ctatgttctt tatttgttag tattagttgc
       61 cctattttta ttaattccaa tattaattta cgccttccga aaatctgact ggaaagatcc
      121 aaactctgat ttcgaaccat tttcttggga aaaaagaact agtaattaaa ttttccataa
      181 gctacaaaag atgaggttaa atatgtttag tgctgaatct ccaattggtg taatagactc
      241 aggtgtcggt ggattgacat cggtaaagga aataattaat ttgcttcctg gtgaagatat
      301 tatctattgt ggagataata tgaatgcacc ttatggcaac cgttctgccg atgatatcat
      361 cagtttgact aaaaaaatgc tgactttttt acagtcaagg aatgtcaaac tagttgctgt
      421 cgcatgtaat actatatcct caactttgga atccgaagaa tacagcggat atgctaagag
      481 ctttccgttt cctatcttat ccatcattga acctgcagta gaagatgtga ttcgccagca
      541 atataaaaat gttggtatta tcgctaccga gtttactatt aaaacggggt gtcacaaaga
      601 gctaataaaa aaattgaatt caacaataaa tgtatttgga gaaccaagca aaaatcttgc
      661 aatgttaatt gaggaaggta atcttaatgc tcctgcaatc ttgaatgaca tcaaaaaaca
      721 tgttaaccat ttaatttccc tccatccagt aaatgaaatt attctcggct gtactcacta
      781 tccaattgtt cagaattttt ttgaagcagt tgcaccggat ataaaattta ttaatcctgc
      841 acatgatcag gctatctcta ttaaaaatca cttgggacag ctaaatttat taaactcctc
      901 aaatatagga actttgcaaa taaatactag tggaagtatg gagatctaca aaacggtact
      961 aggggaattg agcattacga aacctcatac attcagtatc aggcagtttt aaatttatcc
     1021 ggtacaccgt aaaatcccgt ccttctgaac tgcaccccaa aagttggaca ccaactgagt
     1081 aaggtgcatt ttatggacaa acccagttac atactgggta gtgcatccaa ttagtagaac
     1141 atgtgttttt cgataaacgc tccgatcact ttttcgtgga tctcaactga gcgtgagaag
     1201 cagattgttt tacgacccaa ccgcttaatg cgggtgcgta gcgtcagatt attacgctca
     1261 atgcgttggg tgaatatttt gccggtcaga tgcttattct tcggcacctc ccggccatag
     1321 ctgccccagt catcgctggt cagcatgccg atgttgaagg gtgtaagcag tgccagtagc
     1381 tcccggcacg tttgatcggt tcggggacca aaagtgtagg ccagtacacc gcctgttttg
     1441 gtgttgtacg cgtacccgag ccaatgttgc cgggctttac tgccaacgta gctccattgc
     1501 tcatcaagct cgcagataaa cgccacatca gcatgggcaa caggcgaaga agttattcgc
     1561 tttggcgcga gttttttaaa gtccggatga cggtgttact accaattttc agtgtcctgg
     1621 cggtatcgcg aaccccggca ccattaaagg ccatttcagt tatcagctct ttcatacccg
     1681 gcttacgtgc ttgataagta taagtgaact gaaacacacg gtggcagtca cggcagcgaa
     1741 gtctgtcacg gcctttaggg ttctgaccat ggcggtaaac ctgagctgac tgacaacggg
     1801 gacaatgaat attaacgctg gtcatgagat aacctcaaaa gcccgtatta tacatcagat
     1861 tcaactaatt agaggcatca cccatccagc attataaaac atactaacaa taactaaatt
     1921 ataataatca caacttacaa tcaaataata aatgcataag attaacaatg attttttcgg
     1981 gttatattta tctaaaaaag cagaacatta tgtaacttat tattttctaa gcgaaaattt
     2041 attgtgcggt cagactaaca aacttgcgta ccacccgaag aacatgcgaa gatttaagta
     2101 ttctttcttt tttggttttt atttttcatt ttctcttttc ttctctttcc ggttctgttc
     2161 tggttttggt tttttccttt ttatttttat tctttcttca gggttatgct aaaacaccaa
     2221 aacatttttc accacaaaag catccaatca tcttgccaga ccaatcacct taaccggata
     2281 cactgccgga tataatccca ctaacaccca gacaccaagc accatacctg ataactaccg
     2341 tattcaggaa ggaaatgcga gtgatttcac cgtcaccggc acaaccgatc cgccaaaaag
     2401 aggcgtacca atgtcgatat agtcccccgc gcggacaatc acttcgtcaa tgactgccaa
     2461 cgggagttgt tgtagctgtg ggcgcaacaa catgcccaga gctttaccaa agtcctgccc
     2521 ggccaccacc agcaggggat gcggattcgg aaaacgcgcg acgaaatcga ccagcgcgtt
     2581 gatgaccgtc agaaccgcgg cgtaacgcac aggcagcgag gcgggaagcg ccagcacgta
     2641 cacgtcagtt ttgggatcaa gatccagctg aagcagcgcc tgttgccagg cactcaccag
     2701 atccgtttca tcaatcggga tcgccaccgg caaattgcgc agcggcagtt gtacgccctc
     2761 cagccagatt gtgctgccag agagcgaaag ggtatgtgca cccgcgccaa tcaccgtgga
     2821 gcgtacggtt tgcgccggaa actgcacatt catctcacgc aggcgcggat ggtcatgcag
     2881 cgccgttgcc agaagtgggc caatatcggc aaaacagaac gggttggcgg gctggtggcg
     2941 ataacattcg cccacgcctc cagaaagcgt aatgatttcg ggcgtaacac ctgcgggcag
     3001 caaaccggtt tgcatcaatg cctgcgcgag cggcgaaagc gttccgtcaa tcacttcgac
     3061 aatcagcgcc gccatccgcc gggtaacctg caccagctgc gcgccggtca gcgaacgggc
     3121 gtcagtgcct gcaccgaagc actcatccac aatcatctgc cccggtttat gagcgtaaac
     3181 cacgcgcccc tggctgtcgg tttccagcag gcgaccaccg acgttgagac aggcagtgcc
     3241 gctgattttt ccggcatcga acagggcgta gttcgcggtg ccaccgccga tatcgatatt
     3301 cagtacccga cacagccgtt gttcagaaag ggtttgcgcc ccggctccgt gaccggcgat
     3361 cacggattcg aggtgcggcc cggcgctggc gacgacaaaa tcgcccagcg actgtttttc
     3421 cttcatcact ttttccatca gctgtgcgac caggctttcg gtaaactggc cttccggcag
     3481 ctgggcaatg atggtttcgc ggatgcgctg gctttcggtt ttgtcggctg gcacgctggc
     3541 gggcacggac gccgttaccg ggatcgattc atcacattcg gtgatggtaa aacccagtag
     3601 atccgccact tcacgggctt ccggggtaat aatgctggcg cgcagaacca cggacattgc
     3661 ctgttcgccg cgcgcgtgtg cttcacgaat atcattcgct gtgatgagtt ttttcacctg
     3721 cttcctcctg tttggtgaga gatgccagat aatccaccag ttgctgcacg ctttgcgggt
     3781 cgtggctgtt tagctcgaaa atcggctctt cgaaacccgt ctcaagcaat aactttcgcg
     3841 ttgcggcgac gtcagcatct ggcatgtccg ttttactgat gacggcgatt tgccgtttgc
     3901 tgacgccaat atccagcaac ccggcaggca accgactttc cgggtcattt gcgccatgga
     3961 catagatcag catgtctaca tcctgtagcg tggtaattaa ggcgtgatac cagcggggat
     4021 ggctaaaata ttcacccggc gtatcaatat cgcccttatc attaaattcc acggccagtg
     4081 tttttctggc gagggtataa ttcccctgca acgcattaaa taacgttgtt tttcctgccc
     4141 cgacgctacc cacaaacgca attcgtttca tgaatgcctc gtattaactt ttggtcattt
     4201 cacaaagcgt ataatttaat aagcgaccta aaccgctgac ggtttgtgat aacgcttctt
     4261 ctactgcgcc caccgagcca taaatcacca atgcaccgct gaagcggtcg agaaaaccga
     4321 tatgcacatc agcggctttc agcgctaaat caccggcgat catcgccgtt tcgccgggag
     4381 ttagcgtcat aatgccaatt gcgcccgcat ccggaacgcc gattttcttc gccagttcct
     4441 caccgggatg cgcgatgaga tgcgccagtg tgacctgttt gccgggcaca aattcctgaa
     4501 tgatgcgttc tttatccatg agtcgccgcc tgtgaaaaac tttcgtgtat cgtgacaatt
     4561 tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg tagatcaata
     4621 agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat gaatataaat
     4681 aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac tcagtaataa
     4741 gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta agcgtgagag
     4801 ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc caccatattg
     4861 acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc cacgcccatc
     4921 agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt gtaactaatg
     4981 cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa agggctgtcc
     5041 ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat ttcaccatca
     5101 atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat tttgctcgac
     5161 gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg cggctcaata
     5221 ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc ttctgcgtct
     5281 ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag cagcagcgca
     5341 ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt caccacacta
     5401 aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc cccacgctga
     5461 accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc ctgttcctga
     5521 gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt aaagcgcgga
     5581 tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa gcccagtttc
     5641 tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc cagtcccagc
     5701 gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg cagaactacg
     5761 cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt ggttttgtaa
     5821 acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg cgtcgccacg
     5881 cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa gcgcggatca
     5941 aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc atacgctgaa
     6001 gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac cgccgccagt
     6061 ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa gatgaacggg
     6121 aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca gatgatggca
     6181 tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt cgccagcgcc
     6241 aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac tttcgggccg
     6301 gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg tttgccgtca
     6361 tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc ctgatagata
     6421 acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc taccagcagg
     6481 ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac gtcggagatg
     6541 tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat tgccgtgccg
     6601 tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg ttcaatatag
     6661 aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg ttcgagcgcg
     6721 gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc aaatacatca
     6781 atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt gcctgccagc
     6841 gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc aaccaggtta
     6901 ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag acaaggtgcg
     6961 gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg cttggttgga
     7021 gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact ttgttttaac
     7081 tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt gtgggcaaca
     7141 gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga ctttgagcaa
     7201 gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac tgatgttacc
     7261 tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt tagcgaaatt
     7321 tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga gtatttccta
     7381 tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc tgcgatattg
     7441 agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg ttactcaagg
     7501 ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg ggtaaaaaaa
     7561 atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg gtcaatttcg
     7621 gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat gcacgcctct
     7681 cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg tatcagcaac
     7741 aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa ggaattcgcg
     7801 cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg ttttctttta
     7861 cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt gtagggcgta
     7921 tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa gaagatccgg
     7981 gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat gaaaccattg
     8041 tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc tgcgatcgac
     8101 tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca gtggtacgta
     8161 aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa gcggagttcc
     8221 gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc attcgtctgt
     8281 tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa accagccacg
     8341 gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag tatggatgct
     8401 gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga tattgccgaa
     8461 gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta tgatcgcgac
     8521 cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct acatctgacc
     8581 ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc gaaaacccca
     8641 ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc gcttggacaa
     8701 ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc gcagtttaac
     8761 cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga cggctgcctg
     8821 atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact aggcaagctg
     8881 attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg ctggtttacc
     8941 gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga aatcgacggt
     9001 cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa agataagccg
     9061 tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc aggtaaggaa
     9121 gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca aaaactgggc
     9181 tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga tgcccgtgaa
     9241 aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa aaaggctcat
     9301 ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa ggactgggag
     9361 aaaacgactc agaaatatat caatgagtta caggcaaatc cggcgaaaat cgctacccgt
     9421 aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt gctcggcggt
     9481 tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc gctgaaggaa
     9541 gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac cgctatcgcc
     9601 aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct gatgtttgtt
     9661 gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca gattatggtt
     9721 tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc tgttgagcaa
     9781 ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga tcagatggaa
     9841 gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc actgatcctc
     9901 tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga gattgctcgt
     9961 ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat tgccaccggt
    10021 tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg tcgcaatgta
    10081 cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga atatcgggag
    10141 tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat tgccgattac
    10201 tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta cggggaatct
    10261 gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat tgtggcaaaa
    10321 gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc tgtatgttcc
    10381 cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt tttagggccg
    10441 catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg gcccatgcca
    10501 gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac cgtcaaagcg
    10561 ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg tcaccagttc
    10621 acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct cctggccgtt
    10681 gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac gcgacacaat
    10741 ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca tcagtgcttt
    10801 atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg agcagcgcag
    10861 accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga gtgagagtgg
    10921 cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg gtacgctgag
    10981 aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct tttgctgagc
    11041 atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat tgccgtcgcg
    11101 taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg ttgccgtatc
    11161 atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg ccaacggctc
    11221 accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg acttcaaccc
    11281 accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc tcagggtcat
    11341 caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat tattacaggt
    11401 gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag acgtcggcaa
    11461 caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga ccccagaagt
    11521 tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca atcggatttg
    11581 tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc cattaaatgt
    11641 gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc gccggttttg
    11701 atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc atcttcaaca
    11761 ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa gtggattagc
    11821 tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc gcccacttca
    11881 tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc gttatccagc
    11941 agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca ggtagcgaca
    12001 cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag gatcgtcgca
    12061 ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc tttgcgcgtg
    12121 agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt gtctttaatg
    12181 agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta tattacgctg
    12241 tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta cccccagcca
    12301 gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca ccagatccgc
    12361 accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt aacggtactg
    12421 gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt gcgactccag
    12481 ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg ctggcatgac
    12541 aaattcactg ccctcaatcg gtacaggtcg ccgacgcccc tgggcatccg gctcgccaag
    12601 acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct caagcgccac
    12661 cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca cctctttctt
    12721 cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac tcgcgccgtg
    12781 gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta ccacgacgtt
    12841 aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca tcacctgttt
    12901 agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt cattgggtaa
    12961 acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt attgttccaa
    13021 aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt gaatccccat
    13081 cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga aagaaggaat
    13141 gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca ccgccacgcc
    13201 attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga taatcgccac
    13261 ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg ccaacgcctg
    13321 atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat cgcgaatagt
    13381 acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt ccggcagggt
    13441 gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca ccagttcaat
    13501 ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt gctcgccgaa
    13561 cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca gataaatttc
    13621 atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac gcgcgggcgt
    13681 tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg tactggcatg
    13741 ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca tgccggagag
    13801 tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac aggcaggacc
    13861 gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt ttcctgccgc
    13921 gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc aggatttaca
    13981 gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga ttgcgccatt
    14041 agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg cactacgttg
    14101 ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca ggacatgttg
    14161 ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca gacattgctg
    14221 actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta aaaaccttat
    14281 tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt tcatgtgccc
    14341 agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat cactgactaa
    14401 attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc gtgtgattgt
    14461 taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc tgctgtcgat
    14521 tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg ctgaggctat
    14581 caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact tgcaaagtgg
    14641 cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt caccggtatt
    14701 aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg cgcatctgaa
    14761 tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt ggtatcaggc
    14821 caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac agcactacgc
    14881 tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg gtattttcac
    14941 gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc tgaatcagct
    15001 ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc tggataccag
    15061 tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga gcgagctgca
    15121 taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc tccacgagac
    15181 caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa caccagccag
    15241 attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga agcagctgaa
    15301 tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc aaaccctgag
    15361 ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga actgcactgg
    15421 caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc gatgctggga
    15481 cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt gatggaagaa
    15541 cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc ttacttacgt
    15601 atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc acaaagtatc
    15661 atggccgatt tttcccaggc gttgaatgat gcttatcggc agttacgcga gctgttgact
    15721 actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga aatgctggat
    15781 acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc aaccctggca
    15841 ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt gctgaatgcg
    15901 atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc ggacggcaat
    15961 cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga acccgaaggt
    16021 cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac gctgactttt
    16081 tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc agagggtgag
    16141 gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc cctctccctt
    16201 taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt cagcaatctc
    16261 atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt gccgctacag
    16321 tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt aatggcgaat
    16381 ttctttattg atcgccccat ttttgcctgg gtgctgacaa tcctgttgtg tctgacaggt
    16441 accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc accgaatgtg
    16501 cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac cgtgacccag
    16561 gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc tcagagcagt
    16621 ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc ggacgaagcc
    16681 gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa
    16741 aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat tgccttcgtc
    16801 tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag taatattcag
    16861 gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc gcaatattcc
    16921 atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc taaagatgtc
    16981 actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg tggtacacct
    17041 tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct gcaaacacca
    17101 gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt aaggctgggc
    17161 gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg cttcaatggt
    17221 aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat ggcgacagcg
    17281 gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg actggaatac
    17341 aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt ggtgaaaacg
    17401 ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca aaacttccgc
    17461 gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt ctccgtactt
    17521 tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct ggcgatcggt
    17581 ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa
    17641 ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg ggcactggtc
    17701 gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg cggcaccacc
    17761 ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct gtcagtactg
    17821 gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact gaaaaaaggt
    17881 gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa ccgcaacgcc
    17941 gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt
    18001 tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac gtcgttctta
    18061 ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg ttcaacccaa
    18121 caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca tgaaaaagac
    18181 aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg gcaaaacgtg
    18241 gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac cggcacctcg
    18301 tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc tcgcgttatc
    18361 gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga tatggagttg
    18421 caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca gttgctggcg
    18481 ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga tgacagtccg
    18541 cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga tatcgacgat
    18601 attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt tatggatcgc
    18661 ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct gccagatgac
    18721 atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc tgctttcgcg
    18781 acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta ttctgcggtt
    18841 gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga tattatggaa
    18901 tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat gtcgtatcag
    18961 gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct ggtggtattc
    19021 ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat gctggtcgtg
    19081 ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga aaacgacgtt
    19141 tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc
    19201 gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc gacgctccac
    19261 gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat cttcggcgta
    19321 ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt gggtactggc
    19381 gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc gctgttcttt
    19441 gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg
    19501 acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag cgggttcttg
    19561 ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa gtctttccag
    19621 ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat tctacggaaa
    19681 caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc cgggggacaa
    19741 accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac agcaacattt
    19801 cgagatattc atacggcatc taatactgat ttaattctgg ttaaaata
//
LOCUS       DAZXUL010000079        19698 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZXUL010000079 DAZXUL010000000
VERSION     DAZXUL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.079"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACH3L1_002762"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACH3L1_002762"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFT4077439.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACH3L1_002763"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACH3L1_002763"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFT4077440.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACH3L1_002764"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACH3L1_002764"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFT4077441.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="ACH3L1_002765"
     CDS             6038..6658
                     /locus_tag="ACH3L1_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFT4077442.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="ACH3L1_002766"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="ACH3L1_002766"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFT4077443.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACH3L1_002767"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACH3L1_002767"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFT4077444.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACH3L1_002768"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACH3L1_002768"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFT4077445.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="ACH3L1_002769"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="ACH3L1_002769"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFT4077446.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10068..11069)
                     /locus_tag="ACH3L1_002770"
     CDS             complement(10068..11069)
                     /locus_tag="ACH3L1_002770"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFT4077447.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     ELLYRSMKFVSARNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="ACH3L1_002771"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="ACH3L1_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="ACH3L1_002772"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="ACH3L1_002772"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFT4077448.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="ACH3L1_002773"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="ACH3L1_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFT4077449.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACH3L1_002774"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACH3L1_002774"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFT4077450.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="ACH3L1_002775"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="ACH3L1_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFT4077451.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="ACH3L1_002776"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="ACH3L1_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFT4077452.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="ACH3L1_002777"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="ACH3L1_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFT4077453.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5506 c         5031 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAZXUL010000080        19481 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZXUL010000080 DAZXUL010000000
VERSION     DAZXUL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19481
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.080"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..356
                     /locus_tag="ACH3L1_002778"
     CDS             <1..356
                     /locus_tag="ACH3L1_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFT4077454.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="ACH3L1_002779"
     CDS             complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="ACH3L1_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFT4077455.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="ACH3L1_002780"
     CDS             complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="ACH3L1_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFT4077456.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3111..3263
                     /gene="mokC"
                     /locus_tag="ACH3L1_002781"
     CDS             3111..3263
                     /gene="mokC"
                     /locus_tag="ACH3L1_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFT4077457.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="ACH3L1_002782"
     CDS             complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="ACH3L1_002782"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFT4077458.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="ACH3L1_002783"
     CDS             complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="ACH3L1_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFT4077459.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6879..7283
                     /locus_tag="ACH3L1_002784"
     CDS             6879..7283
                     /locus_tag="ACH3L1_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFT4077460.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7309..8022
                     /gene="msyB"
                     /locus_tag="ACH3L1_002785"
     CDS             7309..8022
                     /gene="msyB"
                     /locus_tag="ACH3L1_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFT4077461.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8171..8737
                     /gene="satP"
                     /locus_tag="ACH3L1_002786"
     CDS             8171..8737
                     /gene="satP"
                     /locus_tag="ACH3L1_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFT4077462.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8772..9359)
                     /gene="mog"
                     /locus_tag="ACH3L1_002787"
     CDS             complement(8772..9359)
                     /gene="mog"
                     /locus_tag="ACH3L1_002787"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFT4077463.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9474..10427)
                     /gene="tal"
                     /locus_tag="ACH3L1_002788"
     CDS             complement(9474..10427)
                     /gene="tal"
                     /locus_tag="ACH3L1_002788"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFT4077464.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10706..12136
                     /locus_tag="ACH3L1_002789"
                     /pseudo
     CDS             10706..12136
                     /locus_tag="ACH3L1_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            12206..12982
                     /gene="yaaA"
                     /locus_tag="ACH3L1_002790"
     CDS             12206..12982
                     /gene="yaaA"
                     /locus_tag="ACH3L1_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFT4077465.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13135..13431)
                     /locus_tag="ACH3L1_002791"
     CDS             complement(13135..13431)
                     /locus_tag="ACH3L1_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFT4077466.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="ACH3L1_002792"
     CDS             complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="ACH3L1_002792"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFT4077467.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="ACH3L1_002793"
     CDS             complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="ACH3L1_002793"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFT4077468.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="ACH3L1_002794"
     CDS             complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="ACH3L1_002794"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFT4077469.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18354..18473)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="ACH3L1_002795"
     CDS             complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="ACH3L1_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFT4077470.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18688..19374)
                     /locus_tag="ACH3L1_002796"
     CDS             complement(18688..19374)
                     /locus_tag="ACH3L1_002796"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFT4077471.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         4700 a         5137 c         4751 g         4893 t
ORIGIN      
        1 attatcagtt tcatcatata agatttgact gcatccaggg cgagcaaggt gaattagttt
       61 ttgaacccag tgacgttgaa tattactttg aaccagtgac gatacaggag tcaggaacca
      121 gcatattaaa aaatgagtta gaaaacagtg aggacggtgc gggtcaaata ggctttcaac
      181 tctccaatga tggtacgaat gaaatccagt atggtaaaag caattactac tcttttcagc
      241 atccccatga aggaagtaat cagattcctc tatttattcg cccgcgtaca tatggaaaca
      301 atgtctcaag cgggcaaata atgagccgag tcaaaatcgt ggtaatgtac aactaatcta
      361 tctcccccac cttaactttg ggggagctgc cgattaacgc accgctggac taaaaagcgc
      421 agaataatcc gtattgcaga ttcgctgtac cgccgggtgc tgaatcatcc gctcagcaaa
      481 aatagcatgg tactcttcca tcacattctc gacgcgacca atttctacga cagttttatc
      541 ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag attgcattgt gcatcgcacc
      601 aaaagctttc atcaaagcgg catcatcaaa ctcgccgagg atttctacgt ttaatccctg
      661 ggagttaaac cagttaagca atttgcgccc taacattgaa cgtcgcccag gaatcaaaag
      721 tcgccgttct tccagacaag ccgggaacgg tttttctggt ggtggatttg tacaccagaa
      781 actcacgcca cattcgccaa ttctcacgga gaacaggcct tcctgctgcg tagagtctat
      841 cggacagtca gaaatgatca tatccagttt atgctgactt aattgctcca gcagcatttc
      901 gtgggtggat tcgaagcagc gaagatgaat gggctcgcct tctaccactg cggcgttaag
      961 tacgctactg accaggcgtt tggaaagtgc atcagccacg ccaacgtcaa acaataaatt
     1021 ggattctttg cgatagttca caatatccag catttcctgg cttaaggtga acattttatc
     1081 ggcatagcga tagaccagtt ctcccagctc gctgggttcg agaccacgtc ccttgcgttt
     1141 aaataatttg ccttgcaggc gctcttccag cgctcgaatc tgtccggtaa tggtttgtgg
     1201 agttaaataa agcgcctccg ctgcgccaac cacggaacct tctttataga catgccagaa
     1261 gtaatacaag tggttgtaat tgatatgaga catgctcatt tctctccctg ataacaatga
     1321 aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc aaactgatgg acgcaaacga
     1381 acgcgtaacc agctgtatcc aattaccgcc gaagagatag aaccgaccag gataccgagt
     1441 ttcgcccagt taatcagttc tggatctacg ctaccaaagg ccaggctggc aataaagata
     1501 gacatagtaa aaccgatacc gcacaggatc cccaccacca taatttgctg ataagtcgtt
     1561 ccctcaggca gatgcgccag tttcaaacgc agcgccaacc agcagaacag actaatcccc
     1621 agcggtttgc caatcagcaa gccagcgatg atccccaatg gcagaatgga ggtcaagcca
     1681 tccagcgtga cgccttgcag tgaaacgcca gcattagcaa atgcaaacag cggcaaaatc
     1741 agatacgcca cccacgggtg caacacatgc tccagtcgct tcgctggaga acgcccatgc
     1801 ttctctttca aaggaataaa gaagccgaca attacccccg ccagagttgc gtgaaccccc
     1861 gatttcaaca ccgcagtcca caacaccacg ccaacaagaa tatagacgcc cgtgcggcgt
     1921 acaccacaca gattcaatac cgcgagtacc gcaattgcta cagccgcgac gccaagggag
     1981 gccatcgata agtcattagt gtagaacaat gcgatgataa tgatggcccc aagatcgtcg
     2041 ataatagcca gagccatcaa aaagatcttc agcactaacg gaacacgact tcccaacagc
     2101 gccagtacac caagtgcaaa agcaatgtca gtagccgccg ggatcgccca cccttcgcgg
     2161 gtaatcggat cggcatagtt aaaagccaga tagagtaatg ccggcacaat catcccacca
     2221 atagcggcga taactggaaa tgcggcctgg cgtaagctgg ctagcgatcc ttgcatcagt
     2281 tcacgtttaa cttccagacc gactaacagg aaaaataccg ccatcagcgc atcatttatc
     2341 cataacagca tgtttttgtt gatttcgagt gaaccaaccc ggagctgaac cggcgtctcc
     2401 agaaagtcgt gataccatcc actggttgcg ccgctgttgg ccataatcat cgccaggata
     2461 gcggcaatga taagaataat gcctcccgag gcatcactgc taaagaatcg atgcagatgt
     2521 ttcacttttt atttctcttt caggtgaata gatcgttttt actattttac cccgcacgaa
     2581 tcatcgttaa aatcagatta tagtgtagga ataattcgtt ttttacgagc cattgaagaa
     2641 tttgcgccat cagcggtgag atgcgttttt gacggctgta agcgctcacg agtgacccta
     2701 agcccagata atcatagctt aaggtgagga ttcctgaggg attaattagg aaaagtaata
     2761 attaatttgt tgtcagtcgg cgatgcttgt taggccaata tttcaaaaat aatccagctt
     2821 gtcgtcaata attgcgatga agagataaat taaagttaaa taattaaatc attaaaaaat
     2881 tacgccacaa aaatgcgagg cgtcttcagg attagaatcg ataaaacaaa atgggttaaa
     2941 gagagcgata taatagcggc gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg
     3001 cagatagaga aaagccccga gtgatatttt accatcaacc cgaggcctcc tatatgctga
     3061 acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc
     3121 ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg
     3181 taacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt
     3241 tcacggctta cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg
     3301 tgctcgcata tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg
     3361 gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga
     3421 gcgcgggctg ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag
     3481 ctgtttctgc ttttcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc
     3541 accctgtgcg ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc
     3601 ggtctgggtt tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat
     3661 ttcgccaccc agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc
     3721 ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg
     3781 tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac
     3841 ccctgccggg attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca
     3901 tttgttgcac ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg
     3961 tacagcgaag aatccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt
     4021 ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt
     4081 cggaatgcgg atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt
     4141 atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc
     4201 gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac caccgccgcc
     4261 catgcctcct tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga
     4321 gtcggtcaga acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc
     4381 accctggtta cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttctgatttc
     4441 acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt
     4501 ttttaaattg cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca
     4561 gtataattac ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt
     4621 cgacaacatc gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct
     4681 gggcatgttg ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca
     4741 ttttcgcttc gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg
     4801 cagactcgat agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct
     4861 tacgggtgct gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact
     4921 tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt
     4981 tcagaccaga agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg
     5041 cggaaacgtg caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc
     5101 cgcgcggtgc cgggttgata ccatctaggt tgaactgacc cagagatttg ttatcagccg
     5161 cacgtttacg ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg
     5221 tagagaacac ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca
     5281 tcacaccgcc catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt
     5341 cttttacgtc accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt
     5401 ccgggttaac gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca
     5461 ttggcatacg agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc
     5521 cagcatcctg cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca
     5581 ggctttccag tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg
     5641 cagtgatgta tggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt
     5701 tttctgccgc ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct
     5761 gatctttctt gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac
     5821 ccaggtgggt atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt
     5881 cgatttcgat aatagaaata tcgaaagtac caccccccag gtcataaacc gcgatagtac
     5941 ggttgccagt gcctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga
     6001 tacgttttac ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat
     6061 cgttaaagta tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt
     6121 cagcggtttt cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct
     6181 ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga
     6241 tggaaacatc acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg
     6301 caaacagggt gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag
     6361 tttcaccatc ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct
     6421 ccagcacgcg aggaatggtg ccatccataa tcgctacaca agagttggta gtacccaggt
     6481 cgataccaat tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt
     6541 gtgagtctgt aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag
     6601 actcactcac tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat
     6661 gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa
     6721 ttgacagcaa ttttattcat ataaagaatg aatcgcagcc aaggcgttca tcaaaaaatt
     6781 gtaataaaaa gaaaagatta cgtgcctgaa tcttctcttt atcagcagta aactagtggg
     6841 tattcatccc cctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt
     6901 ccgccagcct ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac
     6961 tcctcggcgt cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg
     7021 tttgtcgcat tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagtg
     7081 gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag
     7141 aaataaaaga agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac
     7201 gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc
     7261 tgaaaattat cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat
     7321 tacctgaatg attcagatct ggattttctt cagcattgta gtgaggaaca gttggcaaat
     7381 ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc gcctctccag catactgatg
     7441 cgcaacgaac tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag
     7501 ctgattgccg gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga
     7561 cacggtaaat tgtatcgggc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc
     7621 gacaaagaga tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat
     7681 acctggaaga aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg
     7741 gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt
     7801 gtgtcgcatt tgctttccag ccaactgacc cgcattttac gcacccacgc agcaatgagc
     7861 gtacttgggc atggtttgct gcgcggcgcg gggctgggag gccctgtggg cgcagcatta
     7921 aatggggtta aagcggtcag cggcagcacc tatcgcgtga cgattccagc cgtactgcaa
     7981 atcgcctgcc tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa
     8041 attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc
     8101 gccctgaaaa cactacagtt attcagggaa attatttcac cattcattcg atgatgattt
     8161 ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt
     8221 cggcatgacc accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat
     8281 tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga
     8341 gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct
     8401 gacgctggtt gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca
     8461 gttccttggt gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac
     8521 tctgaaaggc gcgcgcgtcc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct
     8581 gctggcgatc ggtaacattg ccggtaatgc cgcgatcatc cactttgccg gctggattgg
     8641 tctgatctgc ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt
     8701 tggtcgcacc gttctgccga ttggtgaatc ccactaattg tcttgagatc cccccgctcg
     8761 gggggatttt tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc
     8821 acttctggtg ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg
     8881 gcaaaaatac catgcaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct
     8941 ttaatagact tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacgccc
     9001 acctgacgcg aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca
     9061 ccaaagccag gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga
     9121 cgcgccgggc cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc
     9181 tcacacaacg tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca
     9241 aacggcgtgg ttagcgccga tgtcagccat tcttccagcg ctggaatgcc tttatcctga
     9301 taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc
     9361 aagatttttc cgtttaaaca atctcgttaa acagaatgat acactgcgaa gggagtgaca
     9421 gacaggcttc agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc
     9481 agatcgccga tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc
     9541 gccagtttat ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg
     9601 atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct
     9661 tcgctctccg ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc acagcctgcc
     9721 agttccagaa tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg
     9781 tgctctttgt agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga
     9841 gcgtactctt tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaacggc
     9901 gagatcagga acacgcccgc ctccgcacaa gcacgagcct gagcgaagga gaacagcagg
     9961 gtcaggttac agttgatacc ttctttttcc agctgttctg cagcacggat accctgccag
    10021 gtagaagcca gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg
    10081 atcaggcgtt ttgctttcgc aattgacgct tcagtgtcat aggaaagacg cgcatcaact
    10141 tcagttgaga tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt
    10201 ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg
    10261 acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa
    10321 gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg
    10381 gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc
    10441 tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt
    10501 cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact
    10561 gcgttgatat cgcccaccat ttttatacaa aacctcatgt atgctacgca gaagttatca
    10621 agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc
    10681 gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct
    10741 ttggggatcg gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac
    10801 cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca
    10861 tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt
    10921 gggtagcggc aacctggccg gcgttgcgct ggcaattacc gccggtggac caggtgccgt
    10981 cttttggatg tgggttgctg cgtttatcgg catggcgacc tcgtttgccg aatgttccct
    11041 tgcacaactt tataaagaac gtgacgtcaa tgggcagttt cgtggcggac cggcatggta
    11101 tatggcgcgc gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat
    11161 cgcctacggc ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt
    11221 ttcttttgat tttcctccgc tggtgacggg cattattctc gctgtctttg ctctgctggc
    11281 aatcactcgt ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc
    11341 gataatctgg gtactgacca gcctggtgat ttgcgtaata aatatcgggc aacttcccca
    11401 cgtcatttgg tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc
    11461 gggatatacc ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga
    11521 ggtgggaatg ggatcgacgc caaacgcggc agcggcagct gcatcctggc ctccgcatcc
    11581 ggcagcacaa gggattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac
    11641 ggcaagcgcc atgctgatat tactggcggg taacggcaca acctacatgc cgctggaagg
    11701 tattcagctt atccagaagg cgatgcgggt gctaatgggt tcctagggtg ctgaatttgt
    11761 taccctcgtg gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga
    11821 aaacaatctc ttctttttac gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg
    11881 caccttcgca acggtcatcg gcggcacctt gctaagcctt ccgctgatgt ggcaactggc
    11941 agatatcata atggcctgca tggctattac caatttgacc gccattttac tgctctcgcc
    12001 tgtggttcat accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt
    12061 gtttgatccg ttgcgttatc cggatatcgg tcgccagctt tctccggacg cgtgggatga
    12121 tgtttcgcag gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg
    12181 cataaatttc ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga
    12241 ttaccaaagc ccgttgacca ccacgcgcta tacgctgccg gagctgttag acaattccca
    12301 gcagttgatc catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgcat
    12361 cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat gactggcagc cagatttcac
    12421 gccggcgaat gcccgccagg cgattctggc gtttaaaggt gatgtctaca ccggcttgca
    12481 ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc
    12541 cggcttgtat ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg
    12601 gatccgtctt gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac
    12661 caacaagctg aacgaggcgc tcgcagcaca aggcgataat gtggtgatta atctggcgtc
    12721 agatgagtat tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccggt
    12781 gttcctcgat gagaagaacg gcaaatttaa gatcatcagc ttctacgcta agaaagcacg
    12841 cggtctgatg agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactgactgg
    12901 ttttaatagc gaaggttact tctttgatga agattcctcc agcaatggcg aactggtgtt
    12961 taaacgctac gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg aacgccttat
    13021 ccggcctaca aaagcatgca gattcaatat attgcagaat taacgtaggc ctgataagac
    13081 gcgccagcgt cgcatcaggc gctgaatgcc ggattgttac ccggcatttg tcatttagcg
    13141 gtgatgtttg cctggaccat gaccgccgtg atgatcatga ggagctttct tatggtggcg
    13201 cggcggtggc ggcggtccgt gtgggtgcca gcgattgcct cgccattcat aatgttgttt
    13261 ccaccagccg tggtcgcgcc agtgacctcc atcccagtaa tagccacgat tatcacgatc
    13321 gcctatctgt aattttactg acgggactaa cgtaatttcc gcagcctgtg ctgccatggg
    13381 agcgaccaga accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc
    13441 cttttatcat tgcccgttat gggtcgatat ccggagatta cgcgatgaga acgacggttg
    13501 ttattctccg caatcctaat ctctaagtta ccgcatttat tgataatttc tcctttttcc
    13561 ctgtcgtttt tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg
    13621 cctgattgag ataatgaata gattttactg atgattcatc atcaatttac gcaacgcaga
    13681 aaaatcggcg ggcagattat gtgaaagcaa gggtaaatca gcacgttctg ccagctcttt
    13741 tggcagatcc aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg
    13801 cgcggtgccg aggaacaagc catattcgcc tggattcaac tggtcacgca gcgcacgata
    13861 cgctacggca gcgtgcggct ccgaggtgta gcccagttct tttaactcac gcattgtctg
    13921 ttgcgtggtt tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg
    13981 gcggaacaac tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga
    14041 taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt
    14101 atcgttcacg ttggtcgcag caataaaacg tttcaccggc agaccgaatg acttcgccag
    14161 cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg
    14221 cgcctcctgc ggcagctgcg caacagcttc aaagtagtag caaatctgcg ccagcaaacg
    14281 gctgatgtta atcgagttag ccgagtttaa ccccagcgcc actttcagtt cttcatcatc
    14341 aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc
    14401 gatattgccg cccaacgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg
    14461 atagaggata accactttca cattcggcaa accgtagaaa gcatgagcca ctgcagctcc
    14521 ggtatcaccg gaggtcgcgg tcagaatggt cactggctta tcatccgcaa tatgggtcag
    14581 catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa
    14641 ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc
    14701 gcgcacgcgc tcttccagga tttcctgtgg gatttcatca ccaataaacg ccgagaggat
    14761 cttcgcactg cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa
    14821 ttccggcagg tcgtgcggaa aaaacagccc ctgatttttg cccaacccct gggttacggc
    14881 ttgcgcaaag ctgacctgct cgttgtgatc tttcagattg tagagtttca tttagttttc
    14941 cagtactcgt gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg
    15001 caggtagttc ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacatag
    15061 agcgaacaag gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac
    15121 cgcctgccgc gcctgccgga agccaggcag taaccgttca cggtagggtt cagcgataac
    15181 atctttcatc agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc
    15241 agccagatgt cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc
    15301 ccgggcttct gccgtcgaga ctttaattcc cggatacgcc agcacccaca gccactcatc
    15361 aaaccctggc acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc
    15421 aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc cttccagctc
    15481 gcccatcaaa gccagcaaac gagtgtcatt aagcggcttg ccgcagtgtt cattcatcgc
    15541 catcagcgcc gcgaccaccg aacaggcgct ggagcctaag cccgaaccga tcggcatatt
    15601 cttttccaga gtcatcgcca ctggaatttg cttgccaagc tcctggcaaa aacgctccca
    15661 gcactgataa acgatatttt cccgtggttc tgacggcagc ttatcggcaa agcgtccgag
    15721 tttgttgaga ctgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc
    15781 atcaacgggt gccaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga
    15841 agccggggca taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca
    15901 gatcggcaaa gacaccggca gcggtaacgt cattgcccgc accatatccg cgcagcacca
    15961 acggcagcgg ctgataatag tggctataaa aggccagggc gttttcgcca tttttcactt
    16021 tgaacagcgg atcattccca tccacttcgg caatcttcac gcggcagaca ccatcttcat
    16081 caatattgcc aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg
    16141 caaagagatc gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt
    16201 taaactctgc gggcagcaca ggttcaattt caatatccgc cagctccagt tcacgtcccg
    16261 tttcacgagc gagaatcaat agtttacgcg ccacatccat accagaaaga tcatctcgcg
    16321 ggtccggttc ggtataaccc atttcccgcg ccagcgtggt cgcctcggag aaactcatgc
    16381 cttcgtctaa cttgccgaag atataagaaa gcgaacctga aagaatgccg gagaacttca
    16441 ccaattcatc acctgcattg agcagatttt gcaggttctc aataaccggt aatccagccc
    16501 caacgttggt gtcatagagg aatttacgcc gcgatttttc cgccgcataa cgcaactgat
    16561 gatagtaatc catcgacgag gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt
    16621 cgcgcaggaa gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga
    16681 ccgggttcag cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct
    16741 ctttggcttg tgcaagttct tcctgccagt tttccagatt aaggccatgc acactagtga
    16801 gcagagcctt cgagttggca acgccgcaga cacgtaagtc gatatgttta ttcttcagcc
    16861 agctttgctg acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca
    16921 caaacacttc gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag
    16981 tggtcgcatc atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa
    17041 tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac
    17101 gcaaggtgcg cataccatca cctaccaccg agataatggc cagccgttcc gtcaccgcca
    17161 gcggctccag taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag
    17221 ctcgcacaca gtcgctttgt ggaacgcaga aactgatact gtattcggaa gatgattgcg
    17281 taatcagcac cacggaaata cgggcgcgtg acattgcagc aaagacgcgc gccgccatgc
    17341 caaccatccc tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg
    17401 aaatgccctt gaccggtaat tcgtcttcat cacggctggc accaatgagc gtacctggtg
    17461 cttgaggatt tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa
    17521 tggtgcgggg gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg
    17581 acatcgactt caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt
    17641 caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg
    17701 agtagtcgga accgttacgt ccaagcacca ccagttcgcc tttttcatta ccggcggtga
    17761 aacctgccat caacaccatg tgatcagccg gaatgcggct tgccgcaata cggcgggtgg
    17821 actcagcaat atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg
    17881 gatcgataac ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgatcg
    17941 acattttctc gccacggcaa atcagcgcag cgttgatgct atccgggcac tgccccaaca
    18001 aactaatgcc atgcaggaca tgttttattt gggcaaattc ctgatcgacg aaagttttca
    18061 attgcgccag cgggaacccc ggctgggcgg cggcgagtcc cgtcaaaagt tcggcaaaaa
    18121 tacgttcggc atcgctgata ttgggtaaag catcctggcc gctaatggtt ttttcaatca
    18181 tcgccaccag gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct
    18241 gcctggcatt gctttccaga atatcggcaa cacgcagaaa acgttctgca tttgccactg
    18301 atgtaccgcc gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa
    18361 aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc
    18421 gttacctgtg gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt
    18481 tgtgtactct gtaattttta tctgtttgtg cgctatgcct atattggtta aagtatttag
    18541 tgacctaagt caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgcta
    18601 tcagacactc tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa
    18661 gctgaaaaat acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag
    18721 caaacggtgc aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca
    18781 gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc
    18841 gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat
    18901 taatgttgct aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata
    18961 atccgccacc atcggcacac cagtaagcac gtcagccaac gctaactctt cgttagtcag
    19021 cccggaatct tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc
    19081 taacagcggc accagttcta ctggcgtggc gtagtaatga tatttcgccc gactacgcgc
    19141 agtggtggcg acagtgaaat cgacatcgtg taacgattca gccagtgtcg ggaaaacttt
    19201 aatattatca ataatatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg
    19261 tgcctgacta tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc
    19321 tgccccaata ttttctgctc tggcgggtgc gaccaggata atcgttatac gcatattgcc
    19381 actcttcttg accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg
    19441 aatttacaaa ttcttaacgt aagccgcaga aaaagccctt t
//
LOCUS       DAZXUL010000081        19025 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZXUL010000081 DAZXUL010000000
VERSION     DAZXUL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19025
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.081"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1025)
                     /locus_tag="ACH3L1_002797"
     CDS             complement(<1..1025)
                     /locus_tag="ACH3L1_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4077472.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="ACH3L1_002798"
     CDS             complement(1127..2107)
                     /locus_tag="ACH3L1_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFT4077473.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="ACH3L1_002799"
     CDS             complement(2115..2825)
                     /locus_tag="ACH3L1_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFT4077474.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACH3L1_002800"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACH3L1_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFT4077475.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="ACH3L1_002801"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="ACH3L1_002801"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFT4077476.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="ACH3L1_002802"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="ACH3L1_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFT4077477.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="ACH3L1_002803"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="ACH3L1_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFT4077478.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="ACH3L1_002804"
     CDS             9402..9512
                     /locus_tag="ACH3L1_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFT4077479.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACH3L1_002805"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACH3L1_002805"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFT4077480.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACH3L1_002806"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACH3L1_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFT4077481.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11931..12097)
                     /locus_tag="ACH3L1_002807"
                     /pseudo
     CDS             complement(11931..12097)
                     /locus_tag="ACH3L1_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12394..13026
                     /gene="yihA"
                     /locus_tag="ACH3L1_002808"
     CDS             12394..13026
                     /gene="yihA"
                     /locus_tag="ACH3L1_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFT4077482.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACH3L1_002809"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACH3L1_002809"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFT4077483.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="ACH3L1_002810"
     CDS             16558..17490
                     /locus_tag="ACH3L1_002810"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFT4077484.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..18961)
                     /locus_tag="ACH3L1_002811"
     CDS             complement(17531..18961)
                     /locus_tag="ACH3L1_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFT4077485.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADSAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         4653 a         4803 c         4556 g         5013 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcactggcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgagtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt
    18181 tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt
    18241 ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt
    18301 tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    18361 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    18421 aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg
    18481 caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    18541 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    18601 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    18661 gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga
    18721 gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt
    18781 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat
    18841 cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg
    18901 tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca
    18961 tataaatatt ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt
    19021 ccagc
//
LOCUS       DAZXUL010000082        18744 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZXUL010000082 DAZXUL010000000
VERSION     DAZXUL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18744
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.082"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACH3L1_002812"
     CDS             complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACH3L1_002812"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFT4077486.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1572..1768)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2005..3654
                     /gene="pgi"
                     /locus_tag="ACH3L1_002813"
     CDS             2005..3654
                     /gene="pgi"
                     /locus_tag="ACH3L1_002813"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFT4077487.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4117..4359
                     /gene="yjbE"
                     /locus_tag="ACH3L1_002814"
     CDS             4117..4359
                     /gene="yjbE"
                     /locus_tag="ACH3L1_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFT4077488.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            4473..5111
                     /locus_tag="ACH3L1_002815"
     CDS             4473..5111
                     /locus_tag="ACH3L1_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFT4077489.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5108..5845
                     /locus_tag="ACH3L1_002816"
     CDS             5108..5845
                     /locus_tag="ACH3L1_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFT4077490.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5845..7941
                     /locus_tag="ACH3L1_002817"
     CDS             5845..7941
                     /locus_tag="ACH3L1_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFT4077491.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7988..8266)
                     /locus_tag="ACH3L1_002818"
     CDS             complement(7988..8266)
                     /locus_tag="ACH3L1_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077492.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8536..8946
                     /gene="psiE"
                     /locus_tag="ACH3L1_002819"
     CDS             8536..8946
                     /gene="psiE"
                     /locus_tag="ACH3L1_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFT4077493.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8990..10465)
                     /gene="xylE"
                     /locus_tag="ACH3L1_002820"
     CDS             complement(8990..10465)
                     /gene="xylE"
                     /locus_tag="ACH3L1_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFT4077494.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10836..11726)
                     /gene="malG"
                     /locus_tag="ACH3L1_002821"
     CDS             complement(10836..11726)
                     /gene="malG"
                     /locus_tag="ACH3L1_002821"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFT4077495.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(11741..13285)
                     /gene="malF"
                     /locus_tag="ACH3L1_002822"
     CDS             complement(11741..13285)
                     /gene="malF"
                     /locus_tag="ACH3L1_002822"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFT4077496.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(13439..14629)
                     /gene="malE"
                     /locus_tag="ACH3L1_002823"
     CDS             complement(13439..14629)
                     /gene="malE"
                     /locus_tag="ACH3L1_002823"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFT4077497.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            14994..16109
                     /gene="malK"
                     /locus_tag="ACH3L1_002824"
     CDS             14994..16109
                     /gene="malK"
                     /locus_tag="ACH3L1_002824"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFT4077498.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            16181..17521
                     /gene="lamB"
                     /locus_tag="ACH3L1_002825"
     CDS             16181..17521
                     /gene="lamB"
                     /locus_tag="ACH3L1_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFT4077499.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            17673..18593
                     /gene="malM"
                     /locus_tag="ACH3L1_002826"
     CDS             17673..18593
                     /gene="malM"
                     /locus_tag="ACH3L1_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFT4077500.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         4672 a         4852 c         4863 g         4357 t
ORIGIN      
        1 ttttctgctc attattagct ccttctgatg ccattctatt tcaggaagga gtgtccgtta
       61 aactcaggct acctcatagt gacaagaaaa tcaatacggc ccgaaatata gcttccaggc
      121 catacagtat ttactcaaac aaattactat gcagtttttg caccacctgc tcggcatctt
      181 cgccgggcac caggaagcac aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt
      241 tgaacggttc cagtacgccg aatacctctt tgccaacgcc gcaggctttt gacaggtcat
      301 tgccaatcaa cgcgaccagc gccagacctt cttccacctc cacccggcac agtgcggaaa
      361 gctccatcag cagagattgc gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat
      421 caagggttaa tgccacgctc acttctgacg tggtgattaa gtctaccgaa atattatgcc
      481 gcgcgaggat gccgaaaact tccgcgagga agccgcgaga atgcagcata ttcaggctgt
      541 gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt
      601 cagttttatt gcacaccagc gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga
      661 ccgggatatc gctgcgtact gcgggtagca acgttgccgg atgcagtact tttgcaccaa
      721 aagttgccat ctctgccgct tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa
      781 ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt ccagatatca acacgagatg
      841 cgtgtaaagc ctccgccagc aaggctgccg tataatcgct gcctccacgg ccaagcgtcg
      901 ttgtacgacc tttattttcg ctaccgataa atccctgggt gatcactaag ccttcattga
      961 ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc ggctacatct ggctctgcac
     1021 gaccaaatcg gtcgttggta cgcatcactt tacgtacatc aaaccactgt gcctgaacat
     1081 cgcgttcgcg caggatttcg acaaacagca gggtcgacat cagctcgccg tggctgacca
     1141 gctcatctgt cagcgccgga gacgttgcca gcgccgccgc ttctgccaga acagtaatgt
     1201 tctccagcag acgttcaatc tcttcacgga taacgttcgg gtaacgcaga cgttccagaa
     1261 tggcaaactg gatgttgcga atagcgtcga gtttttcgaa tcgctcgcca ggttccagcc
     1321 cttccgctaa agcgaccagc agattagtga taccagcaga agccgagagg actactaaac
     1381 gcacgttggc atcagaaagc acaatatcag cgctgcggtt catggcgtca aaatcagcta
     1441 cgctggtacc gccaaatttg gagacaacaa tttcagacat aactacctcg tgtcagggga
     1501 tccattttca gccttggcac aagggaagag cggaagacgg gtgggcgcag agcgatactt
     1561 cgctactatt ttcacccaga agtgctccac cacttgcgaa acgcccgact gcgaacgctt
     1621 ctggtgacaa cccaggggat tcagcccctg tagccgatga tgaacgtggc cagccgttca
     1681 atcacctcgg cgatgcaccc cctcaggtgt tatcacagga ctggctcctc caacaccgtt
     1741 acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg tagtacattt ataaataaag
     1801 ggtgagcggg gcggttgtca acgacggggt catgcggatt tttcatccac tcctggcggt
     1861 cagtagttca gctaataaat gcttcactgc gctaagggtt tacactcaac attacactaa
     1921 cggcactaaa accatcacat ttttctgtga ctggcgctac aatcttccaa agtcacaatt
     1981 ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc caacgcagac cgctgcctgg
     2041 caggcactac agaaacactt cgatgaaatg aaagacgtta cgatcgccga tctttttgct
     2101 aaagacggcg atcgtttttc taagttctcc gcaaccttcg acgatcagat gctggtggat
     2161 tactccaaaa accgcatcac tgaagagacg ctggcgaaat tacaggatct ggcgaaagag
     2221 tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg agaagatcaa ccgcactgaa
     2281 aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca ataccccgat tctggttgat
     2341 ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga agatgaaaac cttctcggaa
     2401 gcgattatct ccggtgagtg gaagggttat accggcaaag caatcactga cgtagtgaac
     2461 atcgggatcg gcggttctga cctcggcccg tacatggtga ccgaagcact gcgtccgtac
     2521 aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg ggactcacat ctcggaagtg
     2581 ctgaaaaaag taaacccgga aaccacgctg ttcctggtag catctaaaac cttcaccact
     2641 caggaaacca tgaccaacgc ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat
     2701 gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca atgccaaagc cgttggcgag
     2761 tttggtattg atactgccaa catgttcgag ttctgggact gggtcggcgg ccgttactct
     2821 ttgtggtcag cgattggcct gtcgattgtt ctctccatcg gctttgataa cttcgttgaa
     2881 ctgctttctg gcgcacacgc aatggacaag catttctcca ccacgcctgc cgagaaaaac
     2941 ctgcctgtcc tgctggcgct gattggcatc tggtacaaca atttctttgg tgcggaaact
     3001 gaagcgattc tgccgtatga ccagtatatg caccgtttcg cggcgtactt ccagcagggc
     3061 aatatggagt ccaacggtaa gtatgttgac cgtaacggta acgttgtgga ttaccagact
     3121 ggcccgatta tctggggtga accaggcact aacggtcagc acgcgttcta ccagctgatc
     3181 caccagggaa ccaaaatggt accgtgcgat ttcatcgccc cggctatcac ccataacccg
     3241 ctctctgatc accaccagaa actgctgtct aacttcttcg cccagaccga agcgctggcg
     3301 tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg atcagggtaa agatccggca
     3361 acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta accgcccgac caactccatc
     3421 ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa
     3481 atctttactc agggcgtgat cctgaacatc ttcaccttcg accagtgggg cgtggagctg
     3541 ggtaaacaac tggcaaaccg tattctgcca gagctgaaag atgataaaga aatcagcagc
     3601 cacgatagct cgaccaatgg tctgattaac cgctataaag cgtggcgcgg ttaatcatca
     3661 tgcgtgcctg atgccgatat gtaggccgga taaggcgttt acgccgcatc cggcaaccgg
     3721 agcctgatgc caatatcgac attaatgcat agtatgagga gttaaataac cttaaaaaca
     3781 atgccgacaa taagtcggca ttgttttatc agataaatcc ccttgtctgt gatttaacgg
     3841 aaatcatacc gtgaggttaa tcctaaaata gatttttaat cgttgtttat ttcggagaat
     3901 acgcagatta attacttttg ttttttattt taagcttatg atttttattg ttatttaaat
     3961 ataagttgaa acttatattt gatattcatt ccaattatcc taaaactcca tcgctaattc
     4021 cccgcgccgt aattcgcatg ctttagttgt gtatactcga tcccgcccga aatgtttttg
     4081 ggtaaatctc cattcattca atgaagggaa attgttatga aaaaagttct gtatggcatt
     4141 tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca ggtgggcgaa
     4201 gcggcagagt cggcagcaac gtcggtttcg gcggggagtt cctccgcgac cagcgtcagc
     4261 accgtaagct cggcggtggg tgtcgcgctc gcggcaaccg gtggcggtga tggttctaat
     4321 accgggacca ccacaaccac gaccaccagt acccagtaat aaagtatgta tccccaaaat
     4381 aatttgagtc attgcatctg tggcttgaag tatgaaggga tttaccataa ccacactccg
     4441 gtgtggttat tctgcccctc tggagaagag tcgtgaagcg acctgcactc attcttatct
     4501 gcctgctatt acaggcctgt tcagccacga ctaaagagct gggcaattca ctgtgggaca
     4561 gtctgttcgg cacgccaggc gtacagctga cggacgatga tattcaaaat atgccctacg
     4621 ccagccagta catgcagctt aatggcgggc cgcaattgtt tgtggtgctg gccttcgctg
     4681 aagacggaca acaaaaatgg gtcactcagg atcaagccac tcttgtcaca caacatggtc
     4741 gtctggtgaa gactttgctt ggcggcgaca acctgattga agtgaataac cttgccgccg
     4801 acccgctgat taaacccgca caaattgttg atggtgcaac atggacgcgc acgatgggct
     4861 ggaccgagta ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa tgggatggca
     4921 ccgataccgt caaggtcggc agcgatgaaa ccctggttcg cgtgctggac gaagaagtct
     4981 ccaccgacca ggcgcgctgg cataaccgct actggatcga cagcgaaggg caaattcgcc
     5041 agtcggaaca gtatctcggc gcggattatt tcccggtgaa aaccactctc atcaaggcgg
     5101 caaaacaatg attaaacaaa ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt
     5161 cttcgcggca ggaaccgtca aggtgttcag caatggcagc agtgaggcca aaacgctaac
     5221 gggcgcagag catttaatcg atctggtagg gcaacctcga ctggcaaaca gctggtggcc
     5281 tggtgcggtg attagcgaag agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt
     5341 gctgacacgg ctggcagaac agggcgcaga ttccagcacc gacgatgccg ccgccattaa
     5401 cgccttacgc cagcaaatcc aggcattgaa agtgacaggc aggcaaaaaa tcaatcttga
     5461 tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg ttgcagggca actacacgct
     5521 gtgggtcgga ccaccgccgt ccacggtcac gttgttcggg cttatcagcc atcctggcaa
     5581 tcagccattc actccaggtc gcgacgtggc gagctatctc tctgaccaga gcctgctcag
     5641 cggtgcggat cgcagctacg cgtgggtcgt ttacccggac ggacgcacgc aaaaagcgcc
     5701 ggtggcttac tggaacaagc gtcacgtaga gccgatgccc ggcagcatta tttatgttgg
     5761 cctcgcagat tccgtctgga gtgagacgcc tgatgccctt aacgccgaca ttcttcagac
     5821 tctgacgcag cggatacccc aataatgaaa aaaagacatc tgcttagctt actggcgctg
     5881 ggcattagca cagcttgcta cggcgaaaca tatcctgcgc ccattggtcc gtcgcagtcg
     5941 gatttcggtg gcgtaggatt attacaaact cccaccgcgc gtatggcacg ggaaggggag
     6001 ttgagtctga actatcgcga taacgatcag taccgttatt actcagcttc agtgcaactc
     6061 ttcccgtggc tggaaacaac gctgcgctac accgacgtgc gcacccggca gtacagcagc
     6121 gtcgaagcgt tctctggcga tcaaacgtat aaagataaag ccttcgatct caaactgcgt
     6181 ttgtgggaag agagttactg gctgccgcaa gtggcggttg gtgcgcggga tatcggcggt
     6241 acggggctgt ttgatgcgga atatcttgtt gccagcaaag cctgggggcc gttcgatttt
     6301 acgctcggcc tgggctgggg atatctgggc accagcggta atgtaaaaaa tccgctctgt
     6361 tcagccagtg ataaatattg ctatcgcgat aacagctaca aacaggcggg atctatcgac
     6421 ggtagccaga tgttccacgg tcctgcctca ctgtttggcg gcgtggaata ccagacgccc
     6481 tggcaaccgc tgcgtctgaa actggagtat gaaggtaata attatcagca ggattttgcc
     6541 gggaagctgg agcaaaaaag taagtttaac gtcggggcga tttatcgcgt taccgattgg
     6601 gccgacgtta accttagcta tgaacgtggc aacaccttta tgtttggcgt cacgctgcgc
     6661 accaacttta acgatctgcg cccgtcctac atcgataacg cccgcccgca atatcaaccg
     6721 cagccgcagg atgccatttt gcagcattcg gtggtggcga atcagttaac gttgctgaaa
     6781 tacaacgccg gacttgccga tccgcagatc caggcgaaag gcgatacgct gtatgttacc
     6841 ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca tccgtgctaa tcggatcgtg
     6901 atgaacgatc tgccggatgg gatcaaaacg atccgcatta cggaaaatcg ccttaacatg
     6961 ccgcaagtga cgacggaaac cgatgtcgcc agcctgaaaa atcatctcgc cggagagccg
     7021 ttgggccacg aaacgacgct ggcgcacaaa cgcgtcgagc cagtggttcc gaagtccacc
     7081 gagcagggct ggtatatcga caaatcacgc tttgatttcc atatcgatcc ggtgctgaac
     7141 cagtcggtcg gtggcccgga aaacttttac atgtatcagc tgggcgtgat gggaacggca
     7201 gatttgtggc tgacggacca tctactgacc accggcagcc tgtttgcaaa tcttgccaac
     7261 aactacgaca agtttaacta caccaatcca ccgcaggact cgcacttacc gcgcgttcgt
     7321 acccatgtgc gcgagtatgt gcagaacgat gtctatgtga ataacctgca agccaactac
     7381 ttccagcatc tgggcaacgg cttctacggt caggtctatg gaggttatct cgaaaccatg
     7441 tttggcggtg cgggggccga agtgttgtat cgtccgctgg acagtaactg ggcgtttggt
     7501 ctggatgcca actacgttaa acagcgcgac tggcgtagtg caaaagatat gatgaaattc
     7561 accgactaca gcgtgaaaac cggacatctg accgcctact ggacgccatc tttcgcccag
     7621 gacgtgttgg tgaaagccag cgtcgggcag tatctggcag gggataaagg cggcacgctg
     7681 gagatcgcta aacgctttga cagcggcgtg gtggtgggtg gctatgccac gatcaccaac
     7741 gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag gcgtgtatgt ctcggtaccg
     7801 ttggatctct tctcgtctgg cccgacacgc agccgtgcgg cgattggctg gacgccgctg
     7861 acgcgtgacg gtggtcagca acttgggcgt aagttccagt tgtatgacat gaccagcgac
     7921 cgtagcgtaa atttccgcta agtcatggga aaggtgccag ttttcgcact cactggcacc
     7981 ctcattctta atgacctgtt gagccatcag gaacgcagtt cacccccggt ttacgatctg
     8041 gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga tatcggttgc aggtctggtt
     8101 gcctgagcga ctgggccatc attgggctgg agacattgct ttgcggtagg tcggctttat
     8161 cagcagtacc gagcggacca gcataagcag gaagaacaga gactgataac atcaaagcag
     8221 caaaataagg cttcattttt accaccttta tcaggttacg tttcatttgt tccagaggaa
     8281 cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca cctgaatggg tgatttttga
     8341 attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg ccaggcgcgg cttatccgcc
     8401 ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc aaatccaggt tgaacaaaac
     8461 atacacaaaa aatatagatc tccgtcacat ttttgcgtta tacaggaagc tcgccactgt
     8521 gaaggaggta ctgctatgac gtcactctct cgtccgcgcg tggagtttat ctccaccatt
     8581 ttgcagaccg tactcaatct tggcctgctg tgcctcggcc tgattttggt tgtcttcctc
     8641 ggcaaagaaa cggtgcatct ggctgatgtg ctgttcgcgc cagaacaaac cagcaaatat
     8701 gagctggtag aaggactggt ggtttacttt ctctatttcg aatttatcgc gctgattgtg
     8761 aagtactttc agtccggttt tcacttcccg ctacgttact ttgtctatat tgggatcacc
     8821 gccattgtgc gcttgatcat cgtcgatcat aaatcgccac tggatgtgtt gatctactcg
     8881 gctgcgatcc tgttactggt gatcaccctg tggctgtgta attcgaaacg gctgaagcgg
     8941 gagtaaaaag acagcacgcc gaaatggcgc ggcgtgctgg acaggaagat tacagcgtag
     9001 cagtttgttg tgttttcttc gtttccggtt cccagagcgc ttccagctcc tcaagggttt
     9061 tacctttggt ttccgggaca aatttccaca taaacagtgc tgccagaacg cccatacaac
     9121 cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac cagccaggag tttttgtcca
     9181 tcatcgggaa ggtccaggag acgaagtagt tcgccagcca ctgggccgcc accgcgattg
     9241 ccagcgcttt accacgaata gcattcggga agatttccga cagcagtacc cagcataccg
     9301 gaccccagga catggcaaag gcggcaacat agaacagcat cgacagtagc gccacaatac
     9361 ccgatgcctg agtgtaaaac gcggtaccga ggctaaacat accgattgcc attccgagtg
     9421 cgccgataat ttgcagtggc ttacgaccaa atttatccac agtcataatt gccagtacgg
     9481 taaaggtgag gttgataact ccgacaataa tggtctgcaa cagcgcgata tccgtgctgg
     9541 cccccagcgt tttgaacact tccggcgcgt agtacagcac cacattgatg ccgacaaatt
     9601 gctggaagat ggagagcata acgccgatta caatcacgcc cacgccaaac atcagcagac
     9661 gaccaccggt tttgcggcca tgatccaggg agtgtttaat ttcctgtact gcctgagttg
     9721 caagcgtgtt gcccataatt ttgcgcagga tactttccgt ctgttcttgc ttgccgcgtg
     9781 acatcagcca gcgaggactt tctggcacgg tatacagcag cattaagaac agcagtgcag
     9841 ggatacattc cgaggcaaac atataacgcc tgccgtcagt attcagccag ctggcatcac
     9901 cggaacgggc aataaaatag tttacgcagt aaactaaaag ttgcccgaaa ataatcgcaa
     9961 actggttaaa tgaaaccagt tttccgcgaa tatgagctgg agccagttcc gcaatataca
    10021 ttggtgaaag cattgaggct aaaccaacgc caataccgcc aataatacga taaataacaa
    10081 attccgggac ataacctgcc agataaatag gtactgtgtt gtccgggttt atagaggtaa
    10141 aaccaagttc tggccaggca gaacctacac cagaaataaa aaacaggaca gcagaaatct
    10201 taagtgaatc acgacggccg aagcggttac tgcaataacc accgagggca ccgccgatga
    10261 tgcaaccaat cagagcgctg gctacgcaaa atcctaacag ggagttggca gcggattcac
    10321 ttaagttttg tggagcaaca aagacggtat tgagtgactc aacagtaccg gaaataacgg
    10381 cggtgtcgta gccaaataat aaaccaccta atgtagcgac taaggtaatc gaaaatatat
    10441 aactggaatt atactgggta ttcattcaga cctgccttag accattctga tcttttatca
    10501 gtgcattgat gttggaattg tcaggcagtt aaggcaatta tcttttttta caatgcgttt
    10561 acgtaatgtc ttttctgtga tcttaattgt gataattatc aaaaattgat gaaacaatac
    10621 tattgccgtg actcagagca cgaaagagaa ttatcgtaag tgggaaaaca aataacgtaa
    10681 aaataataag ttctatgatg ggaatattaa ccggcgaacg attcagattg cagacgaaag
    10741 aaaaaaaggc gctccgtgga gcgccgaata atagtcacaa gttgggataa cgtaagttga
    10801 gggtgcagcg gcataacatt ggcagaacaa catctttaac ctttcacacc acctgccgtc
    10861 aggccgttga ccagccagcg ttgagccagc aagaagacga tggtgatcgg taatgcagac
    10921 atcacggcag cggcggcaaa gtcaccccac aggtagtttt gcgggttgag gtattgctgc
    10981 atccccacgg ccagggtgta gctgtttacg tcacgcagta acagcgacgc gaccggaact
    11041 tcagtaatgg cagcgataaa cgacaggatg aataccaccg ccagaatcgg tactgacagc
    11101 ggcaacagga caaggcggaa ggcctgccac ggtgtcgcac catccagcgc agccgcttct
    11161 tccagcgaac tgtcgatggt ttcgaaatag cctttgatgg tccagacatg cagcgcaatc
    11221 ccacccagat acgcgaaaat tacgccgccg tgagtattca ggccaatgaa tggaatgtac
    11281 tcacccagac gatcaaacaa cgcatacaac gcgaccagtg aaagtactgc cgggaacatc
    11341 tggaaaatca gcattccttt cagcagcgtc gctttgcctg gaaagcgcat acgggcgaaa
    11401 gcgtaggcgc aggtggtgga gagcgccaca atgccaatcg cggaaatccc agcgaccttt
    11461 accgagttcc acagccacag cagtaccggg aatggcggtg gcgtaatgcg accatcagcc
    11521 tgttcaacgc taaaacctaa cgccagtttc cagtgatccc aggagatttg ctccgggatc
    11581 aggctaccgg tcgcgaagtt tccctgacgc agcgagatag cgacgaccat cagcagcggg
    11641 aacataatcg ccgcgataaa aagtagcagt agcaggtgag taataaacaa acgtgctttt
    11701 tgcgatttcg gttggaccat tgccattttt gttatctccc ttaatcaaac ttcattcgcg
    11761 tggctttcag gttcactatc gccagcgcac ccaccagcag gaagatcagc gtggcaattg
    11821 ctgccgccag accgaagtcc tgacccccgc cgccttcaaa agcgatacgg taggtgtagt
    11881 taacaagcag gtcggtataa ccggctggcg tggtcgtgcc aagacgatcc gggccgccgt
    11941 tggttaacag ctgaatcagc acgaagttgt taaagttaaa ggcgaagctg gcgatcatca
    12001 gcggcgtcag cggtttaatc agcagcggca gcgtaatctt aaagaagttc tggaacggac
    12061 ctgcgccatc cattgctgag gcttcataca aatcgtccgg aatcgctttc agcaagccca
    12121 tgcagaggat catcatgtac ggataaccca gccaggtatt gacgataatc agcatcgtgc
    12181 gagcggtggt cggatcgctg aaccaggcgg gcttcacgcc aaacagcgcg ctcaacatca
    12241 tgttgatttc accgaagctc tggttaaaca atcctttgaa aatcaagatt gaaatgaacg
    12301 atggcaccgc gtagggcaga atcagcagga cgcgatagac cgctttgccg cgcaacgctt
    12361 cccactgcac cagacatgcc agaaccatgc cgaccgccac cgttaaaaag acagtgatca
    12421 gcgagaacac cacggtccag acgaaaatgg cgaggaacgg tttctgaatg ccttcgtcgg
    12481 taaagacgcg ggtaaagttt ttccagccgg tggtcacggt gtaaccgggg cttagctttt
    12541 catcccccca gttgccgtcg gcggtaatgg actggtaaaa gccaatttgg ttattcggac
    12601 gatatttcac gccactctga ttattcgtca acgtgccgtc accgtcgagt gtgtagagcg
    12661 gctgcgtgcc agaaaactgg cgcagggagc tcatcatcac tttgttgcca tccggcagaa
    12721 tggcggtaat gtcactcagc gcctgacgat tctgggtaat cacgcgcaga ttcgcgcgtt
    12781 cgccttcggg ctgggcggtc gtttctttca gttgcagttt ttgctcgccg ccaaatttaa
    12841 acgcgtcgga gaggtaattt ttgccggttt cgccgtcgct gagcgccagt tgccactcat
    12901 cgcccgccgg gtaaagacca aagttatagg ttttgcctgc ttgccaggag cgatctaaca
    12961 acacttcctg cgcacgttca aaagtcagct ggttagtgct gctgtagttg gtgaaggcaa
    13021 tggcgatggt gcagaccaga gggaagagga cgaataatcc cattccagcc attcccgggt
    13081 aaacatagcg ccaggcgtag gctttacgat tggcgaaaat atacagcccc gctgaactca
    13141 atatcagcgt ggtaatggcg aacaggtatt ccccttgtgc gtacattaaa acaacaaggt
    13201 aacccaccag caggccgagc agacctagca ctgaccattt cagcgcgtcg ctttgccacc
    13261 aatgtttctt tttaatgaca tccatggggt tcttcctcat tccaggacgg ataaggcttt
    13321 cacgccttat ccgacaacaa ctgcctgatg cgacactgat gcgtcttatc aggcctacat
    13381 gcgtttcagt tttgtaggcc ggacaaggcg ttcacgccgc atccggcatt tcacagcatt
    13441 acttggtgat acgagtctgc gcgtctttca gggcttcatc gacagtctga cgaccgctgg
    13501 cggcgttgat caccgcagta cgtacggcat accagaaagc ggacatctgc gggatgttcg
    13561 gcatgatttc acctttctgg gcgttttcca tggtggcggc aatacgtgga tctttcgcca
    13621 actcttcctc gtaagacttc agcgctacgg cacccagcgg tttgtcttta ttaaccgctt
    13681 ccagaccttc atcagtcagc agatagtttt cgaggaactc tttcgccagc tctttgttcg
    13741 gactggcggc gttaataccg gtgctcagca cgccaacgaa cggtttagat ggttgaccct
    13801 tgaaggtcgg cagtaccgtt acaccataat tcactttgct ggtgtcgatg ttggaccatg
    13861 cccacgggcc gttgatggtc atcgctgttt cgcctttatt aaaggcagct tctgcgatgg
    13921 agtaatcggt gtctgcattc atgtgtttgt ttttaatcag gtcaaccagg aaggtcagac
    13981 ccgctttcgc gccagcgtta tccacgccca cgtctttaat gtcgtacttg ccgttttcat
    14041 acttgaacgc ataacccccg tcagcagcaa tcagcggcca ggtgaagtac ggttcttgca
    14101 ggttgaacat cagcgcgctc ttacctttcg ctttcagttc tttatccagc gccgggatct
    14161 cttcccaggt ttttggcggg ttcggcagca ggtctttgtt ataaatcagc gataacgctt
    14221 caacagcgat tgggtaagca atcagcttgc cgttgtaacg tacggcatcc catgtaaacg
    14281 gatacagctt gtcctggaac gctttgtccg gggtgatttc agccaacagg ccagattgag
    14341 cgtagccacc aaagcggtcg tgcgcccaga agataatgtc agggccatcg ccagttgccg
    14401 caacctgagg gaatttctct tccagtttat ccggatgctc aacggtgact ttaattccgg
    14461 tatctttctc gaatttctta ccgacttcag cgagaccgtt atagccttta tcgccgttaa
    14521 tccagattac cagtttacct tcttcgattt tggcgagagc cgaggcggaa aacatcatcg
    14581 tcgttaatgc ggataatgcg aggatgcgtg cacctgtttt tattttcata atctatggtc
    14641 cttgttggtg aagtgctcgt gaaaacacct aaacggactc tagtttcttt atacggcaac
    14701 ctctttccat cctccttgcc cctacgcccc accgttgctt tgtgtgatct ctgttacaga
    14761 attggcggta atgtggggat gcgcacataa aatcgccacg atttttgcaa gcaacatcac
    14821 gaaattcctt acatgacctc ggtttagttc acagaagccg cgttctcatc ctcccgcctc
    14881 ctcccccata aaaaagccag ggggtggagg atttaagcca tctcctgatg acgcatagtc
    14941 agcccatcat gaatgttgct gtcgatgaca ggttgttaca aagggagaag ggcatggcga
    15001 gcgtacagct gcaaaatgta acgaaagcct ggggcgaggt cgtggtatcg aaagatatca
    15061 atctcgatat ccatgaaggt gaattcgtgg tgtttgtcgg accgtctggc tgcggtaaat
    15121 cgactttact gcgcatgatt gccgggcttg agacgatcac cagcggcgac ctgttcatcg
    15181 gtgagaaacg gatgaatgac actccgccag cagaacgcgg cgttggtatg gtgtttcagt
    15241 cttacgcgct ctatccccac ctgtcagtag cagaaaacat gtcatttggc ctgaaactgg
    15301 ctggcgcaaa aaaagaggtg attaaccaac gcgtcaacca ggtggcggaa gtgctgcaac
    15361 tggcgcattt gctggatcgc aaaccgaaag cgctctccgg tggtcagcgt cagcgcgtgg
    15421 cgattggccg tacgctggtg gccgagccaa gcgtattttt gctcgatgaa ccgctctcca
    15481 acctcgatgc tgcactgcgt gtgcaaatgc gtatcgaaat ctcccgtctg cataaacgcc
    15541 tgggccgcac aatgatttac gtcacccacg atcaggtcga agcgatgacg ctggccgaca
    15601 aaatcgtggt gctggacgcc ggtcgcgtgg cacaggttgg gaaaccgctg gaactgtacc
    15661 actatccggc agaccgtttt gtcgccggat ttatcggttc gccaaagatg aacttcctgc
    15721 cggtaaaagt gaccgccacc gcaatcgatc aagtgcaggt ggagctgccg atgccaaatc
    15781 gtcaacaagt ctggctgcct gttgaaagcc gtgatgtcca ggttggagcc aatatgtcgc
    15841 tgggtattcg cccggaacat ctactgccga gtgatatcgc tgacgtcatc cttgagggtg
    15901 aagttcaggt cgtcgagcaa ctcggcaacg aaacccaaat ctatatccag atcccttcca
    15961 ttcgtcaaaa cctggtgtac cgccagaacg acgtggtgtt ggtagaagaa ggtgccacat
    16021 tcgctatcgg cctgccgcca gagcgttgcc atctgttccg tgaggatggc actgcatgtc
    16081 gtcgactgca taaggagccg ggcgtttaag caccccacaa aacacacaaa gcctgtcaca
    16141 ggtgatgtga aaaaagaaaa gcaatgactc aggagataga atgatgatta ctctgcgcaa
    16201 acttcctctg gcggttgccg tcgcagcggg cgtaatgtct gctcaggcaa tggctgttga
    16261 tttccacggc tatgcacgtt ccggtattgg ctggacaggt agcggcggtg aacaacagtg
    16321 tttccagact accggtgctc aaagtaaata ccgtcttggc aacgaatgtg aaacttatgc
    16381 tgaattaaaa ttgggtcagg aagtgtggaa agagggcgat aagagcttct atttcgacac
    16441 taacgtggcc tattccgtcg cgcaacagaa tgactgggaa gctaccgatc cggccttccg
    16501 tgaagcaaac gtgcagggaa aaaacctgat cgaatggctg ccaggttcca ccatctgggc
    16561 aggtaagcgc ttctaccaac gtcatgacgt tcatatgatc gacttctact actgggatat
    16621 ttctggtcct ggtgccggtc tggaaaacat cgatgttggc ttcggtaaac tctctctggc
    16681 agcaacccgc tcctctgaag ctggtggttc ttcctctttt gccagcaaca atatttatga
    16741 ctataccaac gaaaccgcga acgacgtttt cgatgtgcgt ttagcgcaga tggaaatcaa
    16801 cccgggcggc acattagaac tgggtgtcga ctacggtcgt gccaacctgc gtgataacta
    16861 tcgtctggtt gatggcgcat cgaaagacgg ctggttattc actgctgaac atactcagag
    16921 tgtcctgaag ggctttaaca agtttgttgt tcagtacgct actgactcga tgacctcgca
    16981 gggtaaaggt ctgtcgcagg gttctggcgt cgcgtttgat aacgaaaaat ttgcctacaa
    17041 tatcaacaac aacggtcaca tgctgcgtat cctcgaccac ggtgcgatct ccatgggcga
    17101 caactgggac atgatgtacg tgggtatgta ccaggatatc aactgggata acgacaacgg
    17161 caccaagtgg tggaccgttg gtattcgccc gatgtacaag tggacgccaa tcatgagcac
    17221 cgtgatggaa atcggctacg acaacgtcga atcccagcgc accggcgaca agaacaatca
    17281 gtacaaaatt accctcgcac aacaatggca ggctggcgac agcatctggt cacgcccggc
    17341 tattcgtgtc ttcgcaacct acgccaagtg ggatgagaaa tggggttacg actacaacgg
    17401 cgatagcaag gttaacccga actacggcaa agccgttcct gctgatttca acggcggcag
    17461 cttcggtcgt ggcgacagcg acgagtggac cttcggtgcc cagatggaaa tctggtggta
    17521 atagcaaaac ctgggccgga taaggcgttt acgccgcatc cggcataaaa acaggttgtc
    17581 attatctgaa aggggcgaaa gcccctctga ttatcgggtt tagcgcgcta ttgcctggct
    17641 accgctgagc tccagatttt gaggtgaaaa caatgaaaat gaataaaagt ctcatcgccc
    17701 tctgtttatc agcagggtta ctggcaagcg cgcctggaat tagccttgcc gatgttaact
    17761 acgtaccgca aaacaccagc gacgcgccag ccattccatc tgccgcgctg caacaactca
    17821 cctggacacc ggtcgatcaa tctaaaaccc agaccaccca actggcgacc ggcggccaac
    17881 aactgaacgt tcccggcatc agtggtccgg ttgctgcgta cagcgtcccg gcaaacattg
    17941 gcgaactgac cctgacgctg accagcgaag tgaacaaaca aaccagcgtt tttgcgccga
    18001 acgtgctgat tcttgatcag aacatgaccc catcagcctt cttccccagc agttatttca
    18061 cctaccagga actaggcgtg atgagtgcag atcggctgga aggcgttatg cgcctgacac
    18121 cggcgttggg gcagcaaaaa ctttatgttc tggtctttac cacggaaaaa gatctccagc
    18181 agacgaccca actgctcgac ccggctaaag cctatgccaa aggcgtcggt aactcgatcc
    18241 cggatattcc cgatccggtt gctcgtcata ccagcgatgg cttactgaaa ctgaaagtca
    18301 aaacgaactc cagctccagc gtgttggtag gaccgctatt tggttcttcc gctccagctc
    18361 cggttacggt aggcaacacg gcggcaccag ctgtggctgc acctgctccg gtgccggtga
    18421 agaaaagcga gccgatgctc aacgacacgg aaagttattt taataccgcg atcaaaaacg
    18481 ctgtcgcgaa aggtgatgtt gataaggcgt taaaactgct tgatgaagct gaacgcctgg
    18541 gatcgacatc tgcccgttcc acctttatca gcagtgtaaa aggcaagggg taattacgcc
    18601 ccacagtgct gattttgcaa caactggtgc gtctcctggc gcaccttttt ttatgcttcc
    18661 ttcctgggat atgagcgatt ttttatagta cttcacttct tcttcactaa gaatgtccat
    18721 tatttcaatg cattatcaga gatt
//
LOCUS       DAZXUL010000083        18491 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZXUL010000083 DAZXUL010000000
VERSION     DAZXUL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18491
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.083"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACH3L1_002827"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACH3L1_002827"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFT4077501.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACH3L1_002828"
     CDS             complement(1672..2667)
                     /locus_tag="ACH3L1_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFT4077502.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACH3L1_002829"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACH3L1_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACH3L1_002830"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACH3L1_002830"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFT4077503.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACH3L1_002831"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACH3L1_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFT4077504.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACH3L1_002832"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACH3L1_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFT4077505.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACH3L1_002833"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACH3L1_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFT4077506.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACH3L1_002834"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACH3L1_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFT4077507.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACH3L1_002835"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACH3L1_002835"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFT4077508.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACH3L1_002836"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACH3L1_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFT4077509.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACH3L1_002837"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACH3L1_002837"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFT4077510.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACH3L1_002838"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACH3L1_002838"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFT4077511.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="ACH3L1_002839"
     CDS             complement(17699..18322)
                     /locus_tag="ACH3L1_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFT4077512.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         5309 a         4227 c         3971 g         4984 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc a
//
LOCUS       DAZXUL010000084        18367 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZXUL010000084 DAZXUL010000000
VERSION     DAZXUL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18367
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.084"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..248
                     /locus_tag="ACH3L1_002840"
     CDS             <1..248
                     /locus_tag="ACH3L1_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFT4077513.1"
                     /translation="TDYLHYDGILSQRGGLEIQSQFLRGNEQVYQCQVAFVLPGERVM
                     MAFTYARTTPLTPADMTRWAEIKKNLRFRMRQEVRTN"
     gene            267..3653
                     /locus_tag="ACH3L1_002841"
     CDS             267..3653
                     /locus_tag="ACH3L1_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFT4077514.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            3656..4036
                     /locus_tag="ACH3L1_002842"
     CDS             3656..4036
                     /locus_tag="ACH3L1_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077515.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(4091..4378)
                     /locus_tag="ACH3L1_002843"
     CDS             complement(4091..4378)
                     /locus_tag="ACH3L1_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFT4077516.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            4507..5016
                     /locus_tag="ACH3L1_002844"
     CDS             4507..5016
                     /locus_tag="ACH3L1_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFT4077517.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            5013..6431
                     /gene="phrB"
                     /locus_tag="ACH3L1_002845"
     CDS             5013..6431
                     /gene="phrB"
                     /locus_tag="ACH3L1_002845"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFT4077518.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(6581..8062)
                     /gene="dtpD"
                     /locus_tag="ACH3L1_002846"
     CDS             complement(6581..8062)
                     /gene="dtpD"
                     /locus_tag="ACH3L1_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFT4077519.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            8333..9076
                     /gene="ybgI"
                     /locus_tag="ACH3L1_002847"
     CDS             8333..9076
                     /gene="ybgI"
                     /locus_tag="ACH3L1_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFT4077520.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            9099..9755
                     /gene="pxpB"
                     /locus_tag="ACH3L1_002848"
     CDS             9099..9755
                     /gene="pxpB"
                     /locus_tag="ACH3L1_002848"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFT4077521.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            9749..10681
                     /gene="pxpC"
                     /locus_tag="ACH3L1_002849"
                     /pseudo
     CDS             9749..10681
                     /gene="pxpC"
                     /locus_tag="ACH3L1_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            10671..11405
                     /gene="pxpA"
                     /locus_tag="ACH3L1_002850"
     CDS             10671..11405
                     /gene="pxpA"
                     /locus_tag="ACH3L1_002850"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFT4077522.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            11441..12232
                     /gene="nei"
                     /locus_tag="ACH3L1_002851"
     CDS             11441..12232
                     /gene="nei"
                     /locus_tag="ACH3L1_002851"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFT4077523.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(12229..13275)
                     /locus_tag="ACH3L1_002852"
     CDS             complement(12229..13275)
                     /locus_tag="ACH3L1_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFT4077524.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(13427..14503)
                     /locus_tag="ACH3L1_002853"
                     /pseudo
     CDS             complement(13427..14503)
                     /locus_tag="ACH3L1_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(14500..15231)
                     /locus_tag="ACH3L1_002854"
     CDS             complement(14500..15231)
                     /locus_tag="ACH3L1_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFT4077525.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            complement(15246..17705)
                     /locus_tag="ACH3L1_002855"
     CDS             complement(15246..17705)
                     /locus_tag="ACH3L1_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFT4077526.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            complement(17756..18322)
                     /locus_tag="ACH3L1_002856"
     CDS             complement(17756..18322)
                     /locus_tag="ACH3L1_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4077527.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
BASE COUNT         4522 a         4438 c         5098 g         4309 t
ORIGIN      
        1 aaacggacta tttacattat gacggaatat tatctcaacg aggcggtttg gaaatacaga
       61 gccagtttct ccggggtaac gagcaggtgt accagtgtca ggtggcattt gtattgccgg
      121 gtgagcgggt catgatggcc ttcacctacg ccagaaccac accgctgacc ccggcagata
      181 tgacgcgctg ggctgaaatt aaaaagaatc tgcgcttcag gatgcggcag gaagtcagga
      241 cgaattaatc atacagggaa cgggacatgg aagaggcatt ctgggcggca agacagggtg
      301 atgccctgct gcatacgtcg tttatggcag atgtgctggg ggcggtgctg gaggtggccg
      361 caaatgtcgt tattgatgcc ctcattgtcg gcacaacctc gttactggcg acgtcagtgg
      421 ggatcacgat ggggtgcacg gccgtcgtgc tgaccggttt tgtggcgggt gtggcgatgg
      481 tgtacaccgg cgtgacagaa aaggtcagcg aagcctgttc agccctcgca aatatgcttt
      541 tcccgcctca gattgaaggt tacatcctca ccggctctgg tgacacatgg atcaacagca
      601 aacctgccgc gcgggcagcg gcgactgcgg catcgcggca ggacatagag gcgcaggaag
      661 cgcaggcgaa ggcagaacag gaagaagcgc agcgtcagga ggatgccaga acgttccggg
      721 atgtggccgg ggaatatctg gaaatggcgg gggccatagc cctggcggtt aacccggtga
      781 cagggcctgt gctgatgtcc cgggctctgc acgaccagct cagtacggaa gaagggcgtt
      841 ctgaaatggt ggatggcgtg acacattttg tgtcggaact atggcagccc actgtggcca
      901 gtgcggcccc gggatccacc ccgacccctg acgataaaat cgactgccat aaacatcctt
      961 cctcactgac gcagtttctc gcacaaaaaa aagccgcctt tctggatgac cccctgggaa
     1021 cggcccttaa cctgctgaat ccgggcggta tgctggaaac cgcctttcag ggagtgaatg
     1081 cggttattgg cagcatcagt aatctgttca aaggcgatga cgagccgccc gcagcggaat
     1141 atatcgcgga aggcgcgcgg gatgtgcgta tcaacagcca gccggcgaca cgcagcgggg
     1201 cacgctgtac ctgcgaggcc agggtggtga atgagccggg gaacggggcg tttgtgtcac
     1261 cggatgtgcg cattggcggt ccgttactgg tggtcaggga tatcagaagc ggcaggtcgc
     1321 agataaccct ggtggcaacc gtggcgctga tgtttcttcg cccgggcaaa atgctttcta
     1381 aaatcgcctg ttttgctgcc agtgtcggga tgagcatgct gacgcagaag gccaccagtg
     1441 cccttccgca tccggtcaat gccgccaccg gggccaaata cctggccgat gacgatgact
     1501 tcgatttcag tctgccgggc cactttccgc tggactggca gcgcgtgtac agcagtcgtg
     1561 acgaacgtac ggaaggaatg tttggtcagg gctggagtgt aatgtatgaa gtcagcctcg
     1621 tgtgcacacc gggtagcgca gacgaaaact gcatgacctt tgtgtcgggc atggggcgac
     1681 gcttggatat ggaagccgta ctgccggggg gcgggtttta cagccccggt gaagggctgg
     1741 cggtaaggcg tggtgagcag gggcactggc ttatcagcag cgatgacggg cagtgttttc
     1801 tgtttgaggc tgacccgcac cacccgcagc gccagcggct gaaaatgctg ggcgaccgta
     1861 acagcaactg tctgaatctg tactacgacg accggggccg cattgtggaa atcagcggcg
     1921 aacagcagcg tccgtgcatc cgtctgtatt acgaactggc ggcgcatccc cggcgggtga
     1981 cacagattta tcagcatttc cctgagactg cacctttact gctgcgtcgt tacagctatg
     2041 acgaggccgg ccacctgaac ggcgtgtacg acagcacggg gcacctgctg cgtgagtttg
     2101 cttacgacga aaaccactgc atgacgctgc accgccagcc gggcggcgaa gggtattatt
     2161 accagtgggg ctggtatgaa ggtccggatg acgcggcatg gcgggtgacg gggcaccaca
     2221 cggacagcgg tgaacagtac cggctggcgt gggacctggc gcagcgcagg ctgtgcgtga
     2281 cggacggact ggggcgcaca cgttatcacc agtgggatgc gcagaatcag gtgacggcgt
     2341 accaggacga agccggacag gtgacgacat tccgctggag cgacgaagaa cgactgttgc
     2401 tggggatgac cgaccctcag ggcggcaaat ggcgctatgt gtatgacctg caggggcata
     2461 tcacggagac gcacgacccg ctggggcggg tggagcaggc gcagtggcac ccggtgtggc
     2521 accagccgga aacggaggtg gacgcggcgg gaaacctgct ggggaggcgc gcgggggaaa
     2581 gggccacggc agaaaacagc gtggtgccgt tcaaccggct gatgtcttac cgtggtgtgc
     2641 actaccgtta tgacgaattt ggccgtgcgg tggaaaagga gggccggagc ggaacgcaga
     2701 gttaccgtta tgatgcggaa caccggatgg tggaagtgac gacagcgcgg gggacatacc
     2761 ggtatgtgta cgatgcactg gggaggcgga cggaaaaaca gcacatcagc cctgacggta
     2821 agccgtacaa ccggacaaaa tttttatggg acggaatgcg gctggcgcag gagagcaggc
     2881 cggagggaac aggcagcctg tacatctaca gggaccaggg gagttatgaa ccgctggcgc
     2941 gggtggataa ggcgggcaaa gagggcccga accggatact gtattttcac acggatgtga
     3001 acggcgcgcc ggaagagatg acggacagcg acgggaaaat cgtgtgggag acggggtatc
     3061 aggtctgggg taacacgata caggagaagg accacggcgg ggtggagcag aacctgcgct
     3121 accagggaca gtacctcgac agggaaacgg ggctgcacta caatctgcac aggtactatg
     3181 atccggatgt cgggcggttc atggtaactg acccgatagg gttgagaggc ggtttaaatc
     3241 tctacagtta tgcacctaat cctctaaaat atgctgatcc acttggatta actccatgtg
     3301 ctgttagtaa ccaaaaagcg aatcgtttat tagatagtag tgaaaccaaa gttactgtta
     3361 ggtctagaag tgacgctgaa caattgttta tggatagata tttaggtcat aattataaaa
     3421 atatgacagg tgagtcaggc cccagcacta agaacttaat ggaatatctt actgaaaata
     3481 aaacaaaagc aggttcatat cattgggatg atataaaaga tccttctgta acaaaacctt
     3541 catatagagt gtcaggtcat gggccaggaa atcctgatgg tgatttacca catttacaag
     3601 ttcatcaaca tggtggaagt gttagacata tatttttccc gtgggagaca taataatgaa
     3661 caatctcaat acattattaa gtggaaaggt tttgctcaaa gaaattagtc cattaatgcc
     3721 tggatataga acatggattg aaatttcatt gatcgatgaa ttaaaaccat cttatccttt
     3781 tagattagat gaatatgcta tgatagggca ttcaccttat gccaatgaat gtaataaaga
     3841 tgaagctaaa tttaaactac gaatatcatc atttttagct tctgacatag ataatgaata
     3901 cgatccatca tatgattatg ttggaaaata tgaaattatc gcttcactta atgagttaaa
     3961 aactaggtta agtactcttc atgtaaactt ggagcaattt ataaattcat cggaagatga
     4021 tgagtatcca ttataataag acagaataaa aagctaggaa ggttttaaat cttcccagct
     4081 tttttaatat ttaactttcg ttcagcacac taaacatccc atcccgcagt tcctgcacct
     4141 cgtcactgtc agcgatgatg gtcaggcccc cctccgaaat cttcaccgtg acaccccgtc
     4201 cggtatcaaa tccggcctct ttcagccagt cgcccttaag gtgcagactg ggatgttgtg
     4261 aataataccg ggctataccg gttttgcggt cctggtggcg ggctctgaca tagccgactt
     4321 tatagcggcg ctgtgttttg gaaatagtga cttctggcgt aatatcgtgt tcagccatga
     4381 ttaactacct tgattagttg cttgcgggga gcgcgctttc gtaatcttgc cctgctagcg
     4441 gattaacttc acaaacatca tttaccaacg cctacactta ctcctgtata ccgttcagga
     4501 gcagtaatga atctacaacg atttgatgac agcaccctaa tccgtatctt cgcccttcat
     4561 gagttacatc gactgaaaga acatggctta acgcgcgggg cgcttctcga ttatcacagc
     4621 cgctataaac tcgtctttct ggcccattct cagcccgagt accgcaaact tggcccgttt
     4681 gtggctgata ttcatcagtg gcaaaatctg gatgattttt acaaccagta ccgccaacgc
     4741 gtaattgttt tgctttctca tcccgccaac ccgcgcgatc acaccaacgt tttgatgcac
     4801 gttcagggtt attttcgccc gcatattgat tccacagaac gccagcagct ggctgcgctt
     4861 atcgacagtt atcgccgtgg cgagcaacca cttcttgcgc cgctgatgcg tatcaaacac
     4921 tatatggcgc tttatcctga cgcctggctt tcagggcagc gttatttcga actttggccg
     4981 cgtgtgatta acttgcgcca ttcaggagtt ttatgactac ccatctggcc tggtttcgcc
     5041 aggatttacg tctgcacgat aatctcgcac tggctgccgc ctgccgcaat tcgtctgcac
     5101 gcgtgctggc gctgtatatc gctacaccac gcaagtggga gacgcataac atgtcgccgc
     5161 gtcaggctga acttatcaat gctcaactga atgggctaca aatcgcgctg gcggaaaaag
     5221 gtattccttt attgttccgt gaagtggatg actttgtcgc cagtgtcgaa atagttaaac
     5281 aggtgtgcgc ggaaaacagc gttactcatc ttttttataa ctatcagtat gaggtgaatg
     5341 agcgagcgcg ggatgtggac gttgaaagag cgctgcgtaa cgtggtgtgt gaaggatttg
     5401 atgacagcgt gatcctgccg ccgggggcgg tgatgaccgg caatcacgag atgtacaaag
     5461 tctttacgcc ttttaagaat gcctggctga aacggctgcg ggaagggatg ccggagtgcg
     5521 tcgctgcacc aaaagttcgt agtagcggat cgataaagcc cgcgccatcc attacgctga
     5581 attatcctcg tcagtctttc gatactgcgc atttcccggt ggaagaaaaa gcggcgattg
     5641 cgcaattacg ccagttttgc gagaacggtg ctggagagta tgagcaacaa cgagattttc
     5701 cggcagtgga aggcaccagc cgtttgtccg ccagcttggc aacgggcggg ttatcgcctc
     5761 gccagtgttt gcatcgcttg ttggcggaac agccgcaggc gctggacggt ggggccggta
     5821 gtgtctggct tagtgagctg atctggcgcg agttctaccg tcatctgatg acgtattacc
     5881 cctcgttgtg taaacatcgt ccatttattg cctggacgga tcgtgtgcag tggcaaagca
     5941 atcccgcaca tttacaggcc tggcagaaag gcaaaacggg atacccgatt attgatgccg
     6001 ctatgcgtca gcttaacagc actggctgga tgcataaccg gctacggatg attacagcca
     6061 gttttctggt gaaagatttg ttgatcgact ggcgcgaagg cgagcgatat ttcatgtcgc
     6121 agctgattga tggtgatttg gcagctaata acggtggctg gcagtgggcg gcttctacgg
     6181 gtactgatgc tgcgccgtat tttcgtattt tcaatccgat aactcagggc gagaaatttg
     6241 accgtgaggg cgagtttatt cgtcgatggt taccggagct gcgcgatgta ccagggaaag
     6301 cggtgcatga gccgtggaag tgggcgcaga aagcaggtgt gaagctggat tatccgcaac
     6361 cgatagtcga gcacaaagag gcgagagtac aaacgttggc agcgtatgag gcggcgcgga
     6421 aggggaaata acagacgcgt caggcaatcg agcccagatg ccggatgcgg cgtgaacgcc
     6481 taatccagcc tacaaatttg tgcaaattca ataaattgca ggaaatacgt aggcctgata
     6541 agacgcgtca agcgtcgcat caggcatcgg tgctggccta ttatgactcc agcgccagcg
     6601 cgcggtttct gaatttcagc gcctgataca gccaaatcat cagtaccagt cctacacacg
     6661 ccagtgcgcc ccaggtaatt tgatcaaata cttcaatata tgcattgatg gagtagttga
     6721 tcgccccgga agcatcaaac gaagcctgcg atgtctgatc ggcaatcacg cccgccagat
     6781 agttcgcgat cgcgccagaa agcagcatgt agatgccggt taatacgccg gtcacgcccg
     6841 gaatttcaat gcgcgtaatt tgcgacatgg caaccgggtc gataaacagt tccgcaaagc
     6901 ccatcaccgc gaggcctaac accatcagtg gcagagaaga gtgaccatac attgcggacc
     6961 agcgggcgct taaggtcaga atgcagaatc cggcgctcat caggccaagg ccaagagcaa
     7021 attttcccca gatgcgcacg gtacgattac ctgcgacgct ttctttaacc acccacgcca
     7081 ggaacacacc gcacagcata actgcgaagg cattaatcga ctggaacatc gcggtcggaa
     7141 cggtataacc aaacatatcg cggttaacga agcggtcgat ataaaggcta atcgagctac
     7201 cgccctgttg tgcgaaggcc cagaacaaca tactgaagaa ggtgagcgtc acaatcagcc
     7261 ccagctcctt ccgctgtttc tggttttctg ctttgcgata aatttttgcc agtacaccca
     7321 ggccaataat agtcgcgaca attaaggcgt ataccgacca ctctttccag aacagcacgg
     7381 taatcagtgc aggcgttgcc accagcagaa ccagcagcca tccccagttc ggcaggagaa
     7441 agtttgtcgc acgcagtact tttttgttaa cgccgcgggt atgagtgaaa tgacgattgc
     7501 cacataagaa aatgaccaga cccgcgatca ttccaaccgc cgccaggcca aagcccatcg
     7561 cccagctgta ctcttcctgg gcgtaaccac aggcgatagg tgcgataata gaccccacgt
     7621 tacccgccgc atacatcagc gagaaaccgc catcacgacg cggatcggtt ggctcataca
     7681 gctcgccgag cagacagctt acgttagatt taaacaggcc atagccgcag acgataatcg
     7741 ccagggacag atagaggaat gacggatgga tctcactggc acccagcacc acatgaccga
     7801 tcgccatcaa caacgccccc agcatcaccg ccatgcgatt gccgagaact ttatccgcca
     7861 aaaagccacc gaggattggc gtgacataca ccagcgaaca gtaggcgcta aataactcgt
     7921 aggcgtgagt atcgttgtat tttagttgat tggtgagata gagaatcagc agggcacgca
     7981 tgccgtaaaa gctaaaatat tcccagattt gcagcgcaac gacatagtaa atagcgcgcg
     8041 gttgtgatgc gtgtttattc ataataattc cacaattgac ggcaacgtca cgtaatagtt
     8101 gcgcggcgtg cttgtgtttc cttaaagtta aaactttgat acagatctga ttatttatgc
     8161 aatatgctgt ctgattgcat aaatatacat tagctgaagc gtgatgatat aaacaatttg
     8221 cgtcttttgg cgtcgggttt gtttcttaat atgtcaaaag attggctatc gacgaacagg
     8281 cgatgtaacg ctatgttaac ggtgtagcta tcagaatacg gaaggcgcga agatgaaaaa
     8341 caccgaactg gaacaactga ttaacgaaaa actgaacagc gcggcgatta gtgattacgc
     8401 gccgaatggt ttgcaggtgg aaggcaaaga gacggtgcaa aaaattgtta ccggtgtcac
     8461 cgccagccag gctttgctcg atgaggcagt gcgtctgggc gcggatgcgg tcattgtgca
     8521 tcatggctac ttctggaaag gagagtctcc ggtcattcgc ggaatgaagc gtaaccgttt
     8581 aaaaacgttg ctggcgaatg atatcaacct gtatggctgg catttgccgc ttgacgcaca
     8641 tcctgagctg ggcaataacg cacagctggc ggcgttactg gggatcacgg tcatgggcga
     8701 aattgagccg ttggtgccgt ggggagagct gaccatgcct gtgccgggaa tggaactagc
     8761 ctcctggatt gaagcgcgtc tgggacgtaa gccgttatgg tgtggcgata ccggacctga
     8821 ggtggtccag cgcgtcgcct ggtgcacggg cggcgggcaa agttttatcg atagcgccgc
     8881 gcgttttggc gtggatgctt ttattactgg cgaagtttct gaacagacca ttcattcagc
     8941 ccgcgagcag ggattgcatt tttatgctgc aggtcaccat gccactgaac gcggtggtat
     9001 tcgcgcattg agcgagtggc tgaatgaaaa taccgatctt gatgtgacct ttattgatat
     9061 tcctaatcct gcataacgaa taatcagagg gatcgaaagt gcaacgagcg cgttgttatc
     9121 tgataggtga aacggcggta gtgctggaac tggaaccgcc ggtgacgctg gctagccaga
     9181 aacggatctg gcgactggcg cagcgtctgg tggatatgcc gaatgtggtt gaagccattc
     9241 ccggcatgaa caatatcacg gtgattttgc gtaatcctga gtcgctggcg ctggatgcca
     9301 tagagcgttt gcaacgctgg tgggaggaga gcgaggccct ggaacctgag tctcgcttta
     9361 ttgaaattcc ggtggtttac ggtggtgcag gcggaccgga tttggcggtg gtcgcggcgt
     9421 attgcgggtt gagcgaaaaa caggttgttg aattgcactc ctccgtggaa tacgtggtct
     9481 ggtttttagg ttttcaaccg ggcttcccgt atctcgggag tttgccggaa caactacaca
     9541 cgccacggcg cgctgaaccg cgcttgctcg ttccggcagg ttctgtcggg atcggcgggc
     9601 cgcagactgg tgtttatccg ctggcaacgc cgggtggctg gcagttgatt ggtcatacct
     9661 cactcagcct gtttgatccg gcgcgtgacg aacccatctt attacgtccg ggagacagcg
     9721 tgcgatttgt accgcagaag gagggaatat gctgaagatt attcgtgcgg gcatgtacac
     9781 cactgtgcag gatggcggtc gtcacggttt ttgccagtcg ggtatcagcc actgtggcgc
     9841 actggatatg cctgcgctgc gcattgctaa cctgctggtg ggtaatgacg ccaatgcccc
     9901 cgcgctggag atcacgctcg gtcagttaac agttgagttc gaaactgatg ggtggtttgc
     9961 cctgacgggt gcaggttgcg aagcgcggtt ggatgataac gccgtctgga ccggctggcg
    10021 attgccgatg aaagcaggcc agcgtttaac gcttaaacgc ccgcagcacg ggatgcgcag
    10081 ttatctggcg gtcgcgggtg gtattgatgt tccgccggta atggggtcat gcagcaccga
    10141 tctcaaagtg gggattggcg ggctggaagg ccgtttactg aaggatggtg actgactccc
    10201 gattggcaaa tcgaagcgtg attttatgga agcgcagggc gttaaacagc tgctgtgggg
    10261 caaccgcatt cgcgccttgc cggggccgga atatcatgag ttcgatcgcg cctcgcagga
    10321 tgcattctgg cgttcgccct ggcagcttag ctcgcaaagt aaccgaatgg gctatcgttt
    10381 acaggggcaa attttaaaac gcatcaccga tcgcgaactg ttatctcacg gtttgttacc
    10441 gggcgtggtg caggtgccgc ataacgggca gccgattgtg ttgatgaacg acgcacagac
    10501 caccggtggt tatccacgta ttgcctgtat cattgaggct gatatgtacc atctggcgca
    10561 aattccgctc ggtcagccga ttcattttgt ccagtgttca ctggaagagg cactgaaagc
    10621 gcggcaagat cagcaacgtt attttgaaca attagcgtgg cggctgcaca atgaaaattg
    10681 acctgaacgc cgatctgggc gaaggctgcg ccagcgacgc agagctatta acgctggttt
    10741 cctctgccaa tattgcttgt ggatttcatg caggcgatgc gcaaaccatg caggcttgcg
    10801 tgcgtgaagc aataaaaaat ggtgtcgcga ttggcgctca cccgagtttt cccgacaggg
    10861 aaaattttgg tcgcagcgcc atgcagctgc cgccggaaac cgtttacgcc cagacgctgt
    10921 atcaaattgg cgcgctggca acgattgccc gtgcgcaagg cggcgtaatg cgtcatgtca
    10981 aaccgcacgg catgttgtac aaccaggcgg cgaaagaagc acaactggca gacgccatcg
    11041 ccagagcggt atacgcttgc gatccggcat tggttctcgt tgggctggca ggaagcgagc
    11101 tgattcgtgc aggcaagcaa tatggcctga caacgcgcga ggaagtgttt gccgatcgcg
    11161 gttatcaggc tgacggctcg ctggtgccgc gaagtcagcc aggcgcgttg attgaaaacg
    11221 aagaacaggc gttggcgcaa acgctggaga tggtgcaaca cggcagagtc aaaagtatca
    11281 ccggcgaatg ggcaacggtc gcggcgcaaa cggtctgcct gcatggcgac ggcgagcacg
    11341 ctctggcgtt cgcccgccga ttacgagcta catttgccaa aaaggggatt gttgtcgcag
    11401 cataaccccg cttaataaag aatgaaaaaa ggatatcacc atgcctgaag gcccggagat
    11461 ccgccgtgca gcggataacc tggaggcggc gatcaaaggc aaaccactaa ctgatgtctg
    11521 gtttgccttc ccgcagttaa aaacttatca atcacaactt atcggtcaac acgttaccca
    11581 tgtggaaacg cgtggtaagg cgttgttaac tcattttccc aacggtttaa cgctctacag
    11641 ccataatcag ctttacggcg tctggcgcgt ggttgatacc ggcgaagagc cgcagaccac
    11701 gcgagtattg cgggtaaaac tgcaaacggc tgacaaaacc attctgcttt atagcgcctc
    11761 ggatattgag atgttgaggc cggaacaact gaccacgcat ccgtttttac aacgcgttgg
    11821 tcccgatgtg ctggatccga atctgacgcc ggaggtggta aaggaacggt tattgtcgcc
    11881 gcgctttcgt aaccgtcagt ttgcaggatt actgctcgat caggcgtttc tggctgggct
    11941 tggcaattat ttgcgggtgg agatcctctg gcaggttggg ttgactggaa atcataaagc
    12001 gaaagatctc aatgcggcgc aactggatgc actcgcacac gcgttactgg agattcctcg
    12061 gttttcttac gctacgcggg ggcaggtgga tgagaataag catcatgggg cgctgtttcg
    12121 ctttaaggtt tttcatcgtg atggcgaact gtgcgaacgc tgtggcggca tcattgagaa
    12181 aaccacgttg tcctcgcgcc cgttttactg gtgtccaggc tgccagcact aggccgaccg
    12241 cttcggcgca taggttgaaa taaaccgcgc aatggctggc cccgtcagca aaatactgaa
    12301 caggcgcagg gtttgcatcg ccataataag cgccatatcg gcattgctcc ctgcggcgat
    12361 gaccgccacg gtatcaagcc cgccgggact ggtggcgagg taggcagtca taaaatcaat
    12421 atgcataaac cgggtcagtc cccacgccat acctgcacaa atagccagca gagcaaaaat
    12481 cgacagcagg atttgcggta gcgggcgtag tgcccgcagt aagatttgtt tatcgaaacc
    12541 aagaccaatc cgccagccaa ttgccatata cgccatcgcc agcagccatt ccggtagttc
    12601 gatggtgatg agctgaccag actggagcac tgcgcccgcc agcatcggga tgagcatcgt
    12661 gccggaagga agacgcagca tgcatcccgc cgtaccggca acgactgcga gcaaaatcgt
    12721 aagcaggaga ttaatgctca ctggcgggaa ccagacgatt tgctggttaa ccgcttcagc
    12781 gttatcaccc agcatcatgc gggtgaccag aacagcggcc cccgcgacga acagcactcg
    12841 cagatattgc ataaacgcca ccaggcgaat atctgcgccg taatcttgcg ccatagcgac
    12901 cattgccgct gcgccgcctg gagaagaacc ccaggcaccg gtatttccag gcagtgagct
    12961 atagcgcacc aataaccaac ccacgatggc gctggaaagc agcgtcacca gtaaaatagc
    13021 aagcacgatc ggccagttgg cggccagcgt ggtgagaatc gaaccagtga ggttttgcgc
    13081 aatcatgcag ccaagaatgg cctgcgcggc gagaaaagcg gagcggggaa gttgcagagt
    13141 aattccgcgc atactgaaga tgatcccggc gatcatcggc ccaagtaata gcgccgccgg
    13201 gagatgtacc gcgaggaaac caatagaaag gagaagtgat agcacacata acattcccca
    13261 ctgcaaaact ggcatccttc gctccctcat tattttctct ttggtgtatc gatacagcat
    13321 aaagaaagcc gggctaggaa aaaataacag caatcaagga aaaggggaaa atcagcaatt
    13381 ttctgaaaga gatgcccttt ccggctgcaa agggcatcat ggcaaatcag ttatagttaa
    13441 ttttaaaaag caccactgta tcgaagggcc cagcctcgat gggggtacct gcaatggacg
    13501 aaagttcagc tgtataggtt ttttcgtaga ccaacatgtc gctggtgaaa tcggcgtatt
    13561 cagcatattt attgaaggta taaggcgagg cgttttcatc cagcaaacgt agcttcagac
    13621 cattaccaat caacaatgct ttatcttctt caaccaacgt ttcttcggta taaaaggagg
    13681 aactgacttt aaatccgtcg gtgcattggt cattttgtga ttttgtcgtc ttaatactga
    13741 atgttttcga cattgtgtgg cgacgaatat ccagcaaatt aaatttacca aaatcaatca
    13801 tctgtgtttc cggtgaaatg gaaaaattga cgctgcaatc cagcacgcga atgttttcca
    13861 gaccagtaat atgatatttc aggttttggg cagtagggtc ttcgttaacg ctaccggcac
    13921 cgtcaaattg cacgacgata tagtcgctaa gcgtactttg ataatcatgc ggcggcattt
    13981 ctcgtatttt gacgtataac cgcatgcgag cgagaaaggt tcgcgacatg tgaatattag
    14041 cgggatcacc cgagcaaatt ttgttctgcc atcccatcag gggtataaat gtcaatgttc
    14101 ttactgtcga tacactggtt agtgtcgatg cggctttttc cctggttggc gtcataatca
    14161 acgccattgt aagttactcc gagttgataa taacgatcct gcacccccgg gtaggggttt
    14221 acccatacgt agacatgttc gctttcaaag ttgccgttgg tattgttgtc acaatagacg
    14281 ggaattttaa tatcgtcaga ttcccagatc ttattttaat atcgtcagat tcccagatct
    14341 tatcgccgag tttggcattg cctggtacag caaaaggttg aatcgcttct gatttttcta
    14401 ccgagccacc ggatgaacca aagtagcagt tcagggcaag cgcagacttc aacgacagaa
    14461 ataacagaca gatgacgagc aacactccct tgccagcact catttcttcg cctccacggc
    14521 ttgtaaatgg cattgtccgc tgcagttgag cgtcgtcatg cgtaacgcgc cgtaatcatc
    14581 catataaccg agataaaact ggctgccgct ataatttccg gtgtttgtgt tgaccgtact
    14641 aaaaggcgca accatcacgg ttttgaagcc ggggagaacg cctttttcat ttcgtcccag
    14701 gtaggcaatg gtcagataat acgcggtagg atttttcagt gtcagttggt tgccctgctg
    14761 gctgacctgt aactgcagtt cgaccttttc tcctgctttc ttgcgtaatg cagccggtcg
    14821 ccagaacacc ttgatacgac tctgaatggc gacttgcaga acggcatggt cgctgttttt
    14881 ttccggggcg ggaggaattt cacgcatatt gtagaaaaat agcgtttcgc gatcgcccgg
    14941 taattttgtg gtcgaggctt gctttactat ccgtacctgt gttgttgctt tcggttccag
    15001 acgttgtatc ggcggcaggg cgaccagaag atcatcgctt ttctcccctt tttcattttc
    15061 gagccaggaa taagcgaggt aggggagttt atcgctacga ttatccaccc gcagactggt
    15121 agctttatca ttagcgttaa acacaatacg agtgcgatcg ggttgtagtg cagcgtggct
    15181 gcacagactg gcaaccaaca acaaaagggg tagtcctttc ataaatgtca ttttcatttc
    15241 cttaattaat gacaaggtaa aatcaggcga tttgcggtgt cttccatatg ttcaggaaga
    15301 tggagcgagc aatgctggct atcaccccag accacggtaa acttttgatt ttcagcgact
    15361 ccgcttaacc aggcatgtcc ttcctcgcca accatcccca cgctaatgcc gctgtcatcc
    15421 tggcggatat ccgctccgag gggaggaaac tgaccgctgg cgttgcgaat gatgacgtta
    15481 acgtctttac cggaacggga agccagtgat ttgtaaccta tcgcgccttc aatccacgtt
    15541 tctttgataa cgttttctgc aactgttacg ccgtcgggta agtcattcat gttcaccgcc
    15601 acggtggaag gctggtaact ggaaatcaac ggcaccaccg caatgccaaa atggttggtg
    15661 tagtcgagat tgccctgaac cggaatatct gccacgccat cggtgctgac catcaggcgt
    15721 ggttcattgg tggagctgcg gcgatgaaac gctgcaccat attgggttgc ggtgaaagaa
    15781 ccgctccagc tgctgctgac ggaactgtaa tcattggcgg cataggtacc agaaatatcc
    15841 cactcacccg ctgaactcag gtgctgatag ttaccgctca cctgggctcc attgtcagga
    15901 cggtcggatt gcagtccggc agacatgccc cagctattac gttcatccag cgtatcgttc
    15961 cacgacatgc ggtgtgtggt gctgtggcta ctgttttgca tgtcatagcc aacccgacca
    16021 ccgttaccga aggggagcga aatcgacagg taaatctggt tgtcgcgatc tttatcttcg
    16081 taatgggttg tattgaatga cgtagaaata gagatatctc tccagtcacc aatatcaaca
    16141 ttaaaacctg ccgtgatgtt ggcggtcgtg gaggcgtctg cattccacca ggtttgatgt
    16201 agcaggttgg cgtaaagatt gaggtttagt ggggtaatcg gttggcccac agataagctg
    16261 atcgtctgtt tttcgtcctg cgcatcgctg tcgttgtatt tgtgatccag atagttggcg
    16321 tagctgtgaa aatgacgatc ggagaaacga taagcggcga gggaaatcgt gctgttagtg
    16381 gcatccactt gtttgctgta attaaaacgg tagcttaagc cccgctcgtc ctgctgagta
    16441 tcaaaatggc tactggccca cgtgacatca aacgacagcg caccaagcca cagcatattt
    16501 tgcccaatac ccattgcggc agaatggtag tcatcaccag aaagcagcag gccgctgtac
    16561 agcgaggtgt ttgacagcat cccccaggaa acttcattgc taaaaaaggt ttcattttca
    16621 gtttgatgtg acatggaggg ccgtggctga cccgcggcca gtttatagcg aacctgtccc
    16681 tgacgagtca ggaagggcgt cgatgctgct gaaacctgga aattgttcac ccgaccatct
    16741 tcttccgtca ctttgacatc cagtgtgccc tgaacagact gattaaggtc gtcaatgata
    16801 aatgggcctg gtgggacttt tttctggtaa atgacgcggc ctgattgact gatcgtcacc
    16861 gtggcattgg tctgtgcaat accgctaatt tgtggggcgt agccgcgtag ttcccatggc
    16921 aacattcgat cgtcacttgc cagtgccgcg ccggtataag aaaaaccgtc gaaaatattg
    16981 ggactaaaat ccgtttcgcc gagggttaat tttgaaccta attgcggtaa cgggcgaaaa
    17041 agataggtac gtgatatttc gcctgactgt tcatggttat catcgctatc agtatgattc
    17101 aactgataat cactgcgtaa gcgccatgcc cccgtgttga ttccggctgt tccataggcg
    17161 ttaagattag tgctgctgct gccatcctgt gggcggtagc tgctggcaaa caagttgtaa
    17221 tccatcagga caccggcgac accttctttc catgtggagg gtggggtcca gttttctgag
    17281 tgccacgcca gccaggcttg cggaatacta atattcagtt gctgattggc ttgatcgaaa
    17341 ttgaagagca tttcaggtcg ggagctaaaa tcgacgcact gatttatcaa tggtaacgac
    17401 tgacggactt caggttttaa gccaaattta tcgaccagta aatcattgat gcacggaatg
    17461 gttttatcgt catttttgtg ccagttaatc ttttgctcgt tactgatttg gttattattt
    17521 accgcaacgc taacaaaata ctcaccggga gcaatgactc ctttttcctt taataatgaa
    17581 atatcaatgc ggtcgcgcat tgatttatcg agaacattca gattgaattc gaccgccaat
    17641 gcgcaaggca tcgccacgac caggcaggat acaaaagaga gtcgataaat attcacggtg
    17701 tccatacctg ataaatattt tatgaaaggc ggcgatagcg ccgccaaagt aatacttatt
    17761 tataatccag cacgtaggtt gcattcgccg taactgcgcc tggcgttaca ggtgtagcct
    17821 gatcaatttg ttccatccag gcaaagaaat tcagcgtttg ttcgctggag gtcggagcca
    17881 ggtcgatatc tggagtagcg gtgcctaaaa cgatattgct ttgatcttta ttcattaaac
    17941 gtacgccgac gccggtggct tcacccgtgc tggtgttgtt gagaatcgga tctgcgccgg
    18001 tggttttcgc tgagctatca aaggtgactg caactttaga aatcttgcca ccggaaccgt
    18061 tgctggagtt ttccagatca cagttgacta agagcaggtc gacagcaacg gcatcgctgt
    18121 gatgtgactg attaatgtgg ctggtggtca cctgaccgag ttcaacctct ttatcaatat
    18181 catcctgatg tatttcacac ggtgcttcaa taacttcacc tttaaagtga atttcaccgg
    18241 atccttcatc agccgcataa acaggtgctg tgaacagcag agatacggcc agtgcggcca
    18301 atgttttttg tcctttaaac ataactgagt cctttaacag aaaagaaaaa agtatatcgg
    18361 tgcaaga
//
LOCUS       DAZXUL010000085        17993 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZXUL010000085 DAZXUL010000000
VERSION     DAZXUL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17993
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.085"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="ACH3L1_002857"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="ACH3L1_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFT4077528.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="ACH3L1_002858"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="ACH3L1_002858"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFT4077529.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /locus_tag="ACH3L1_002859"
     CDS             2826..3980
                     /locus_tag="ACH3L1_002859"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFT4077530.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="ACH3L1_002860"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="ACH3L1_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFT4077531.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="ACH3L1_002861"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="ACH3L1_002861"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFT4077532.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="ACH3L1_002862"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="ACH3L1_002862"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFT4077533.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="ACH3L1_002863"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="ACH3L1_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFT4077534.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="ACH3L1_002864"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="ACH3L1_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFT4077535.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="ACH3L1_002865"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="ACH3L1_002865"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFT4077536.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="ACH3L1_002866"
     CDS             11296..11511
                     /locus_tag="ACH3L1_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFT4077537.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACH3L1_002867"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACH3L1_002867"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFT4077538.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="ACH3L1_002868"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="ACH3L1_002868"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFT4077539.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="ACH3L1_002869"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="ACH3L1_002869"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFT4077540.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="ACH3L1_002870"
     CDS             complement(16247..16786)
                     /locus_tag="ACH3L1_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFT4077541.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="ACH3L1_002871"
     CDS             complement(16802..17320)
                     /locus_tag="ACH3L1_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFT4077542.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GVAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17631..17822)
                     /locus_tag="ACH3L1_002872"
     CDS             complement(17631..17822)
                     /locus_tag="ACH3L1_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFT4077543.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17840..17992
                     /locus_tag="ACH3L1_002873"
     CDS             17840..17992
                     /locus_tag="ACH3L1_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077544.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
BASE COUNT         4242 a         4415 c         4960 g         4376 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagcta cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagattaa atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa
    17461 catatatatt aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa
    17521 agacgtaaaa tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc
    17581 atccgacagc gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt
    17641 accggtgatt cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc
    17701 caggcaccga cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt
    17761 acgatacggg tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc
    17821 acaaaaggtt ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca
    17881 ggacaaaatg gtcaattctt cgttatgtaa aaaagcgtca gtggatacat attttaatgt
    17941 tatggaagtt aatttaatta tttacaatga tgatgtaata atgatgaatt aac
//
LOCUS       DAZXUL010000086        17947 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZXUL010000086 DAZXUL010000000
VERSION     DAZXUL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17947
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.086"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..2263
                     /locus_tag="ACH3L1_002874"
                     /pseudo
     CDS             1..2263
                     /locus_tag="ACH3L1_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2264..3913
                     /locus_tag="ACH3L1_002875"
     CDS             2264..3913
                     /locus_tag="ACH3L1_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFT4077545.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3918..4694
                     /locus_tag="ACH3L1_002876"
     CDS             3918..4694
                     /locus_tag="ACH3L1_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFT4077546.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4969..7818
                     /gene="rcsC"
                     /locus_tag="ACH3L1_002877"
     CDS             4969..7818
                     /gene="rcsC"
                     /locus_tag="ACH3L1_002877"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFT4077547.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8018..8668)
                     /gene="rcsB"
                     /locus_tag="ACH3L1_002878"
     CDS             complement(8018..8668)
                     /gene="rcsB"
                     /locus_tag="ACH3L1_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFT4077548.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8685..11357)
                     /gene="rcsD"
                     /locus_tag="ACH3L1_002879"
     CDS             complement(8685..11357)
                     /gene="rcsD"
                     /locus_tag="ACH3L1_002879"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFT4077549.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12095..13216
                     /gene="ompC"
                     /locus_tag="ACH3L1_002880"
     CDS             12095..13216
                     /gene="ompC"
                     /locus_tag="ACH3L1_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFT4077550.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13328..14383
                     /gene="apbE"
                     /locus_tag="ACH3L1_002881"
     CDS             13328..14383
                     /gene="apbE"
                     /locus_tag="ACH3L1_002881"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFT4077551.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14457..15521
                     /gene="ada"
                     /locus_tag="ACH3L1_002882"
     CDS             14457..15521
                     /gene="ada"
                     /locus_tag="ACH3L1_002882"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFT4077552.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15521..16171
                     /gene="alkB"
                     /locus_tag="ACH3L1_002883"
     CDS             15521..16171
                     /gene="alkB"
                     /locus_tag="ACH3L1_002883"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFT4077553.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            16247..17890
                     /gene="yojI"
                     /locus_tag="ACH3L1_002884"
     CDS             16247..17890
                     /gene="yojI"
                     /locus_tag="ACH3L1_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFT4077554.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         4327 a         4373 c         4838 g         4409 t
ORIGIN      
        1 ttaacctcgt cgctgaaagg taaacctgtt tctgcgcagc taacggtagg cgtggtcgat
       61 gaaatgatct acgcgctgca accagaaatc gcgccgaata tcggcaaatt tttctatccg
      121 ctggggcgta acaatgtgcg taccagctcc agtctgtcgt ttatcagcta tgaccaggcg
      181 ctctccagcg agccggttgc gcctggcgcg actaaccgca gcgagcggcg agtaaaaatg
      241 cttgaacgtc cacggcgtga agaggtggat accgcggcat ggatgccgtc actcacaacc
      301 gataaacaag gcaaagcgta tttcacgttc ctgatgcctg attcgttaac ccgctggcgt
      361 atcaccgcgc gtgggatgaa cggcgacggg ctggtcgggc aggggcgtgc ttatctgcgt
      421 tcggaaaaaa atctctacat gaagtggagt atgccaacgg tgtatcgcgt gggcgacaaa
      481 ccggcggcag gactgtttat cttcagtcag caggataacg aaccggtggc gctggtgact
      541 aaatttgcag gcgctgagat gcgccagacg ctgacgctgc acaaaggggc gaattatatt
      601 tcgctgacgc agaatattca gcaatctggc ttgttaagtg cagaactgca acaaaatggg
      661 caagtgcagg acagcattag cacaaaactg tcttttgtgg acaacagctg gcccgttgaa
      721 cagcagaaaa atgtcatgct cggcggtggc gagaacgcgc tgatgttgcc cgagcaggcg
      781 agtaatatcc ggctacaaag tagtgaaacg ccgcaggaga ttttccgcaa caatcttgat
      841 gcgttagtcg atgaaccgtg gggtggggtg atcaacaccg gtagccgtct gatcccgctc
      901 agtctcgcct ggcgttcgct tgccgatcat caaagtgccg ccgctaacga cattcgtcag
      961 atgattcagg ataaccgtct gcggctgatg caactggcgg ggcccggagc gcgctttacc
     1021 tggtggggtg aagatggcaa tggtgacgcc ttccttacgg catgggcatg gtacgccgac
     1081 tggcaggcca gccaggcgct cggcgtaacg caacaaccgg aatactggca gcatatgctc
     1141 gacagttatg ccgagcaggc ggataatatg ccgttattgc atcgggcgct ggtgctggcg
     1201 tgggcacagg agatgaatct gccgtgcaaa acgttgttga aaggggggga tgaagctatc
     1261 gcccggcgcg gaactaaaga tgaagatttc tctgaggaag acatccgcga tatcaatgac
     1321 agcctgatcc tcgatacacc ggaatctcca ctggcagatg cggtggcaaa cgtcttaacc
     1381 atgacgttgc tgaaaaaagc gcagttgaag tccacggtga tgccacaggt tcagcaatat
     1441 gcgtgggata aagcggcaaa cagcaatcag ccgctggcgc acacggttgt gctgctcaat
     1501 agcggtggtg acgctaccca gacggccgct attttaatgg tttgaccgct gagcaatcca
     1561 ctattgagcg cgcgctggcc atgaactggc tggcgaaata tatggcgaca atgcctccag
     1621 ttgttttgcc tgcgcctgcg ggcgcatggg ctaaacataa gttaactgga gggggcgaag
     1681 actggcgttg ggttggttag ggtgtgccgg acattctctc ttttggtgac gaattatcgc
     1741 cgcaaatgtg caggtccgct ggcgtgaacc ggcaaaaacg gctcaacaaa gtaacattcc
     1801 ggtgaccgtt gaacgccagt tgtatcggct tatccccggt gaagaagaga tgagctttac
     1861 tctgcagccg gtgaccagca atgagattga cagcgatgcg ctgtatctcg atgaaattac
     1921 gcttaccagc gagcaggatg cagttctgcg ctatggtcag gtggaagtac cgctcccgcc
     1981 gggagctgac gttgagcgca caacatgggg catttcagtc aataaaccca acgccgcgaa
     2041 acagcagggg caattgctgg aaaaagcgcg taatgaaatg ggcgaactgg cctatatggt
     2101 gccggtgaaa gaactgacgg gaacggtcac tttccgccat ttgctgcgct tctcgcaaaa
     2161 agggcaattc gttctgcctc ctgctcgtta tgtgcgttcc tatgcacctg cgcagcaaag
     2221 tgtcgcggca gggagtgaat ggaccgggat gcaggtgaaa taagtgaact ggcgcagaat
     2281 cgtctggcta ttggcgttgg ttactctgcc aacgcttgcg gaggagacgc cgttacaact
     2341 ggtgctgcgc ggtgcgcagc acgatcaact gtatcagctg tcgtcatcag gcgtgaccaa
     2401 agtctcagca ttacctgact cgctcaccac gccgttaggg agtttgtgga aactgtatgt
     2461 ttacgcctgg ctggaggata cccatcaacc ggagcagccc taccagtgtc gcggaaactc
     2521 cccggaagag gtttattgtt gtcaggctgg ggagagcata acgcgcgata ccgcgctggt
     2581 acgttcctgt gggctctatt ttgcgccgca gcggttgcat attggcgcag atgtgtgggg
     2641 gcaatactgg caacagcgtc aggcacctgc gtggttagcg tctcttacaa cgctgaagcc
     2701 agaaacctcg gtaacggtta aatcgctgct tgattcatta gcgacgttac ctgcgcaaaa
     2761 caaagctcag gaagtcttac tggatgtggt gctggacgag gcgaaaatcg gtgtcgcatc
     2821 catgctcggt agtcgggtac gggtgaaaac ctggagctgg tttgccgatg ataaacagga
     2881 aatccgtcag ggtggttttg ctggctggtt aaccgatggt acaccgctgt gggttactgg
     2941 cagtggcacc agcaaaacgg tactaacccg ttatgcgacg gtgcttaatc gggttttgcc
     3001 agtgccgacg caggttgcca gtgggcaatg tgtagaggtg gagctgtttg cccgctatcc
     3061 gctgaagaaa atcacagcgg aaaagagtac gacggcagta aaacccagcg tacttaatgg
     3121 gcgctatcgt gtgacgttta ctaatggtaa ccacattacg tttgtcagcc acggtgagac
     3181 gaccttactg agcgaaaagg gcaaactgaa attgcagtcg catctcgatc gtgaagagta
     3241 cgttgccaga gttctggatc gcgaggcgaa aagtacgcca cctgaggcag cgaaagccat
     3301 gactgtggcg atacgtactt ttttacagca aaacgccaat cgggaaggtg attgcctgac
     3361 catccccgac agcagcgcca cgcagcgtgt ttctgcttcg ccagcgacca cgggggcgcg
     3421 aacgatgacc gcctggacgc aggagctcat ctatgcaggc gatccggttc attatcatgg
     3481 cagtcgcgct accgaaggga cactttcctg gcggcaagcc acggcccagg cggggcaggg
     3541 ggagcgttac gaccagatac tcgcgtttgc ttatcccgac aacagcctta gccgctgggg
     3601 agctccacgc tcaacctgcc agttattacc caaagcgaaa gcctggctgg cgaaaaaaat
     3661 gccgcagtgg cggcgtatat tacaaggcga gacggggtac aacgaaccag acgtgtttgc
     3721 ggtctgtcgt ttagtctcag gtttccccta taccgatcgg cagcagaaac ggctgtttat
     3781 tcgtaacttc tttacgcttc aggatcggct ggatttaacc catgagtatc tgcacttggc
     3841 ctttgatggt tatcccaccg ggcttgatga gaactatatc gaaacgctga cccgacaatt
     3901 attaatggac taatgctatg cgaaagattt ttcttccgtt gttactggtg gcactttcgc
     3961 ctgtcgctca cagtgaaggt gtgcaggaag tcgagattga cgcaccgctc tctggctggc
     4021 atccggcaga gggcgaagat gccagttttt cacaatcgat caattacccg gcgtcgtccg
     4081 tcaacatggc agatgatcaa aatatctcag cgcagatccg cggcaaaata aagaattatg
     4141 ctgcggcggg taaagttcag cagggccggc tggtggtcaa cggtgccagt atgccgcaga
     4201 gaattgaatc cgatggttca tttgcacgcc cttatatttt cactgaaggc agcaacagcg
     4261 tgcaggtcat cagcccggat gggcaaagcc ggcaaaaaat gcagttttac tcaacgccgg
     4321 gtacaggaac tattcgtgca cgtttacggc tggttctctc gtgggatacg gacaataccg
     4381 atctcgatct ccatgttgtc acgcccgatg gcgaacacgc ctggtacggt aacaccgtgc
     4441 tgaaaaacag tggtgcactg gatatggatg tcacgacggg gtacggaccc gagattttcg
     4501 ccatgccagc accagttcac gggcgctatc aggtgtatat caactactat ggtgggcgca
     4561 gcgaaacgga attgacgacc gcccaactga cgctgatcac cgatgaagga tcggtcaatg
     4621 agaaacagga aacatttatt gtaccgatgc gtaatgctgg cgagctgacg ctggtgaaaa
     4681 gttttgactg gtgatccatc ccttcacaca acctggctct ctcttttttc agataagact
     4741 gctgggcgat ctgccgacgc caggatataa ccattaacaa gcgtcggcag attggtcatt
     4801 tcttattttt cgtactgaga actgtccatc tactccggct ttcctggtta aggcaatggt
     4861 ttctaagcga tacgcattat tgcaatggtg acagaacatt gcggtaacac gcttttaccg
     4921 ctaccttaac cacactccat cggtcacctg aggcggagct tctccccttt gaaatacctt
     4981 gcttcttttc gtacaaccct gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg
     5041 ctctggctgt tgattgcttt ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga
     5101 gaatcggaaa ttcgtcagga atttaatctg agttccgatc aggctcagcg ctttattcaa
     5161 cgcacctctg atgtgatgaa agagcttaag tacatcgccg aaaatcgctt atcggcagaa
     5221 aacggtgtgc tttccccgcg tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg
     5281 ctgtttgccg actccgattg ttccgcaatg agtaacacct ggcgaggttc tctggagtca
     5341 ttggcgtggt ttatgcgcta ctggcgcgat aatttttctg cggcttacga tctcaaccgg
     5401 gtatttttaa tcggcagtga taacctctgc atggccaatt tcggtctgcg tgatatgcca
     5461 gtggaacgcg ataccgcgtt gaaagcgttg catgaacgca tcaataaata tcgaaatgca
     5521 ccacaagatg atagcggcag taacctctac tggatcagcg aaggtccgcg cccttgcgtc
     5581 gggtattttt acgcgttgac gccagtttat ctggcgaacc ggttgcaggc gcttttgggt
     5641 gtcgagcaga ccatccggat ggagaacttt ttcttaccgg gtacgttgcc gatgggggtt
     5701 accattcttg atgaaaatgg tcataccctg atttcgctta ccggaccaga aagtaaaatt
     5761 aagggcgatc ctcgctggat gcaggaacgc tcctggtttg gctatacgga agggttccgg
     5821 gagctggtgc tgaagaaaaa tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg
     5881 gttgataagg tgctggaacg cattcgcatg ttgatcctta acgcaatttt gctgaatgtg
     5941 cttgccggag ctgcattgtt tactctcgcg cggatgtacg agcgacgtat tttcattccg
     6001 gcggaaagcg acgccctgcg actggaagaa catgagcagt tcaatcgcaa gattgtcgcc
     6061 tccgcgccag tgggtatctg cattttgcgt accgctgatg gcgtcaatat tttaagtaac
     6121 gaactggcgc atacctatct caatatgctt acgcatgagg accgccaacg actgacgcaa
     6181 attatctgtg ggcagcaggt caattttgtt gatgtcctga ccagcaacaa taccaatctg
     6241 caaatcagct tcgtccattc gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg
     6301 gatgtttctt cgcgcgtgaa gatggaagag tcgttgcagg agatggcaca agcagcggaa
     6361 caggcgagcc agtcaaaatc gatgttcctt gccaccgtca gtcatgagct gcgaacgccg
     6421 ctgtatggca ttatcggtaa cctggatctg ttgcaaacca aagagttacc gaaaggcgtc
     6481 gatcgtctgg tgacggcaat gaacaactct tccagcctgt tgttgaaaat tatcagcgat
     6541 attctcgatt tctcgaagat tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca
     6601 ccgcgtgaag tgatgaacca catcaccgcc aactatttac cgctggtggt acgcaagcag
     6661 ttaggcttgt actgctttat tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg
     6721 cgtttacagc aggtcatctc caacctgttg agtaacgcca taaaattcac cgataccggc
     6781 tgtatagttt tgcatgttcg cgctgatggc gattatctct ctatccgtgt tcgcgatacc
     6841 ggcgtgggga ttccggcgaa agaagtggtg cgcttgtttg atcccttctt ccaggttgga
     6901 acgggcgtac agcgaaactt ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg
     6961 atcagcatga tggacggcga tatctcggta gattcagaac cgggaatggg cagccagttt
     7021 accgtgcgta ttccgttgta cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg
     7081 agtggtaaac gctgctggct ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc
     7141 agtttgcagc gcagcggcat cgtcgttaca acatacgaag ggcaggaacc gactcccgaa
     7201 gatgtgttaa tcactgacga ggtagtgagt aaaaaatggc agggcagagc ggtagtgacc
     7261 ttctgtcgtc gccatattgg tattccgctg gagaaagcgc caggggagtg ggtacacagt
     7321 gtggctgctc cgcatgagct accggcattg ttggcgcgta tttatttgat cgagatggag
     7381 agcgacgatc ctgctaacgc tctgccgtcg acggacaaag cggtcagcga taacgacgat
     7441 atgatgattc tggtcgtgga tgatcatccg attaaccgac gtctgctggc agatcagttg
     7501 ggatcgttgg gctatcaatg taaaaccgcg aatgatggcg tcgatgcgct taatgtactt
     7561 agtaagaatc atattgatat cgtgcttagc gacgtcaaca tgccaaatat ggatggttac
     7621 cgcttgacgc aacgcattcg tcagttggga ctgacgttgc cggtaatcgg agtaactgct
     7681 aatgcgttgg ctgaagagaa gcagcggtgt ctggagtccg gtatggacag ctgcctctcg
     7741 aagccggtaa cgctggatgt gataaaacag acgctgacgg tatatgccga gagggtcagg
     7801 aaatcgcggg attcgtagga cggataaggc gtttacgccg catccggcat tcagtgcaaa
     7861 tgccagataa gacactgacg cgtcttatct ggcctacttt aatgtaatct gttgaaataa
     7921 tgggaatcgt aggccggata aggcgtttac gccgcatccg gcaatcggtg caaatgccag
     7981 ataagacact aacgcgtctt atctggccta cagatgatta gtctttatct gccggactta
     8041 aggtcacaga agagagataa ttcagcaggg cgatatcgtt ctcgacgccc agcttcatca
     8101 tcgcagattt cttctggcta ctgatggttt taatactgcg gttcagcttt ttagcgatct
     8161 cggtcactag gaagccttcc gcaaacaggc gcagaacttc actctctttt ggcgagagac
     8221 gcttgtcacc gtaaccacca gcactgattt tttccaacag gcgagaaacg ctttccgggg
     8281 taaatttctt ccctttctgc agcgcggcga gagctttcgg cagatcggtc ggtgcacctt
     8341 gtttcagcac gatcccttcg atatccagat ccaataccgc actaagaatc gccgggttgt
     8401 tgttcatagt cagaacaatg atcgacaggc ttgggaaatg gcgcttgatg tacttgatta
     8461 aggtaatgcc atcgccgtac ttatcgccag gcatggagag atcggtaatc aacacatgcg
     8521 catccagttt cggcaggttg ttgatcagtg ctgtagagtc ttcaaattcg ccgacaacat
     8581 tcacccactc aatttgctca agtgatttgc gaataccgaa caagactatc ggatggtcat
     8641 cggcaataat tacgttcata ttgttcatgt aataggctac cttgctacag caagctcttg
     8701 acataactgt caatgtcgct gatgtatttt tctattcctg gaacatcctt ctcacgaatc
     8761 agatgttcca gcgtttcaca taactgcttg ccgggtacca gatttagcat ggcaaatacg
     8821 cctttaagac gatgagccgt ttgggctaac gcagcaaagt cactggttgc tgcttcagta
     8881 tacagcctct taacatcatc cggtactgtg tctacaaaga gcgcataata gccgctggca
     8941 tggagttgcg cattttcatc tccgcccaga ggcgattctg tcacctcttc ctgtgccagt
     9001 tgcacttcaa ttaattgtaa gaccgcttcc tgcatagcgt tgctcatatt gaagttgacg
     9061 cacaattgac caggcccaat ttctcgtacg ccagactcat catcgcttaa aagcaagcca
     9121 gaggcagtaa gattagacgg attatccgtt aaaaagatat cataatcttg actaattaat
     9181 ctttcatcgg gtgtgataca ggttgcaccc caattttcta acaggcgagt gacaatattc
     9241 cgaatttctg ccgaagtaac atccaccatt acgcagacat catccagtaa acgctcttct
     9301 tcctcttcaa cttccgggtc agcggcgagc attttgatat gcacagagta gcgtgtacca
     9361 agcccatccc gcgttttgat gtttaaatga ccgcccagtt tacgtgccag ttgatcgctc
     9421 agccagaatg ccagcgggtc cgcctttcca taacgatcgt tttgggtctg gttgataaac
     9481 gggaagtgca aattatccat ttcatgaata cttacgcctt ctcccgtgtc cagaatgcgg
     9541 aatgtcaggc ggtcttcaga ggactcatcc tgatcaacct caagggtgat ttttcccaat
     9601 tgcgttgagg tcacggcata ttgcatcagt agcagcaaaa tacgtcgtaa ggcatcgcga
     9661 tcgccgcggc gcatatcgtg tgctttcaga tgattgttaa tcagcagttg cagaccttta
     9721 cgcttgatgg caggcaacac tgaaggcaca acttcatcaa ttaaatcctg cacggagaac
     9781 agcaccgtct cacttttcca gctatcatcc gcaagcatgt tcgctaactg tatttcttcg
     9841 accagccgca ccagcacatc tgcctgattc gccagttgtt tgctttccgg ggcgttgagt
     9901 ttagccgcac tctccgccag ggactgtgcg ggttctttca gcgcatcgcc aatgtttttc
     9961 ataaaggtca tccgcccctg ctggtttttc tcatacagac gctgcgcctg cttgagtttc
    10021 ttgtttacca gcacttcgcg atcctgatcg cgaataatga aaatttgtgt gcgcggcgcg
    10081 acctggctgc ggaacatgcg gatctcatac agctcgttat tgatcgtcgc ctgaataatc
    10141 ccctgatgct gttccgccat ggtggtgatg ttttgcagat tcagatgcgg cagcaaatga
    10201 tcggcaattt tgttacttat gacagtgcgg ttcgattcct gatcgtgaac cagcaggccg
    10261 agcggcaaca gtgagactat ctcttcattg attgcccgta aaatgcgcaa ttcgttattg
    10321 accgccgtgc tggggacgct ttctgtactg cggctggaga aatggcggaa tgtggtatag
    10381 ccaaataacg ccagcgccag caaaccgatg ttcagcagca gtggcagcag aatgttttgc
    10441 aacgtatcca gcaataaggt gccataagga acctgccaga ccaggcgcat atcggtagag
    10501 ttgagtgccg aggagatttc aatcttcgta ctgttaaagt ggatactgac gctatccgtc
    10561 ccttctttct cattatcatt gtttcccgtt gccgtcgcgt ctggctcaag gcggaaactg
    10621 tccagcggca tacccggtgg gatcaaatca ttaatcggca gatcaaaagc cacgaccgtt
    10681 gccagatgtc ccggctggtt gaacgtggta cgcaaggtaa agtaatgacc gttctgccag
    10741 gctaggcggc gcatgttaga aaaactttcg cgttcatcga gggcgttggc ctgttgtaac
    10801 atttctgcac gacgtgaatc aacaatgtca ctgacggtcg attctttaaa tccggaggtg
    10861 agatctttaa ggggtagggt tgagatcagc accagactgt tatcctgacc attcaggtaa
    10921 tacatcgacc acggtacatt ttctgcaccc cacaatgtat ccagataagt ggacatccgc
    10981 tgagtcatct caagcgttga gttgtcgtga gagccaaaga ttaacgcttc cgttttgcgg
    11041 cgtggtttct ccagatagta gacatcctgt ttcaggcgcg tctcttgtaa accttcgccg
    11101 gaggaggggg aggtcgtcgc ggcaatgttg tcgtagatct gcctggtcac gtaacgccag
    11161 gtatcgacgc gcttttggat tgcgtgggta atgtcgacaa tctggtaact tttatctttc
    11221 agccaagcgt taacggcact ttgtaccatt acccccatcg tcaccagtaa cacaatgatc
    11281 aacagtaaaa agaagcgggt aatgctcccc ggtaggagtg aaaagcgggt cgtggccgtt
    11341 gtctctttct gacgcattcg tgtttatgac ctgttaaaac ttcgcgaagt gatagggcag
    11401 tataaagggt acagtgtgaa attccagact cttacctgat tgatgattct ctattctccc
    11461 gtgatattta tcatcctgta tggggggaaa tagatgtaca agcgccattt tgctgtacaa
    11521 ataacctaca aaaagcccaa cgaaattaat tgcaaataaa caaagattgc tggaaattat
    11581 gctgatgtta attatttgtg aaatagttaa caagcgttat agtttttctg tggtagcaca
    11641 gaataatgaa aagtgtgtaa agaagggtaa aaaaaccgaa tgcgaggcat ccggttgaaa
    11701 taggggtaaa cagacattca gaaatgaatg acggtaataa ataaagttaa tgatgatagc
    11761 gggagttatt ctagttgcga gtgaaggttt tgttttgaca ttcagtgctg tcaaatactt
    11821 aagaataagt tattgatttt aaccttgaat tattattgct tgatgttagg tgcttatttc
    11881 gccattccgc aataatctta aaaagttccc ttgtatttac attttgaaac atctatagcg
    11941 ataaatgaaa catcttaaaa gttttagtat catattcgtg ttggattatt ctgcattttt
    12001 ggggagaatg gacttgccga ctgattaatg agggttaatc agtatgcagt ggcataaaaa
    12061 agcaaataaa ggcatataac agagggttaa taacatgaaa gttaaagtac tgtccctcct
    12121 ggtcccagct ctgctggtag caggcgcagc aaacgctgct gaagtttaca acaaagacgg
    12181 caacaaatta gatctgtacg gtaaagtaga cggcctgcac tatttctctg acgacaagtc
    12241 tgtagatggc gaccagacct acatgcgtct tggcttcaaa ggtgaaactc aggttactga
    12301 ccagctgacc ggttacggcc agtgggaata tcagatccag ggcaacagcg ctgaaaacga
    12361 aaacaactcc tggacccgtg tggcattcgc aggtctgaaa ttccaggatg taggttcttt
    12421 cgactacggt cgtaactacg gcgtagttta cgacgtaact tcctggaccg acgtactgcc
    12481 agaattcggt ggtgacactt acggttctga caacttcatg cagcagcgtg gtaacggctt
    12541 cgcaacctac cgtagcaccg acttctttgg tctggttgac ggtctgaact tcgctgttca
    12601 gtatcagggc aaaaacggta gcccggaagg tgaaggcatg accaacaacg gtcgtgaagc
    12661 tctgcgtcag aacggtgacg gcgttggcgg ttctattact tatgattacg aaggcttcgg
    12721 tatcggtgct gcagtttcca gctccaaacg tactgatgac cagaacttcg gtctgaaccg
    12781 ttatgatgag cgttacatcg gtaatggcga ccgcgctgaa acctacaccg gtggtctgaa
    12841 atacgacgcg aacaacatct acctagctgc tcagtacacc cagacctaca acgcaactcg
    12901 cgtaggcaac ttgggttggg cgaacaaagc acagaacttc gaagctgttg ctcagtacca
    12961 gttcgacttc ggtctgcgtc cgtccctggc ttacctgcag tctaaaggta aaaacctggg
    13021 tgtcatcaat ggtcgtaact atgacgacga agatatcctg aaatatgttg atgttggtgc
    13081 gacctactac ttcaacaaaa acatgtccac ctacgttgac tacaaaatca acctgctgga
    13141 cgacaaccag ttcactcgtg acgctggcat caacactgat aacatcgtag ctctgggtct
    13201 ggtttaccag ttctaatctc gatggatatc gaacaaaggg cctgcgggcc cttttttcat
    13261 tgttttcagc gtacaaacac agttttttgg tgtactcttg cgaccgttcg catgaggata
    13321 atcacgtatg gaaataagct ttacccgcgt ggcactgctg gttgccgcgc tcttctttgt
    13381 tggttgcgat caaaaaccac aacccgccaa aacccacgct actgaagtta ccgttcttga
    13441 aggcaaaacc atgggtacct tctggcgtgc cagcatcccg ggcattgatg ccaaacgcag
    13501 tgccgaactt aaagaaaaga ttcagaccca gctggacgcc gacgatcagc tgctttcgac
    13561 ctataaaaaa gattccgcgc tgatgcgctt taacgactcg caaagtttgt cgccgtggcc
    13621 ggtaagtgaa gcgatggccg atatcgtcac cacctcgctg cgcattgggg caaggaccga
    13681 tggtgcgatg gatattaccg tcgggccgct ggtgaatctg tggggctttg gcccggaaca
    13741 acagccggtt caaattccga gccaggaaca gatcgatgcg atgaaagcca aaaccggctt
    13801 acagcacctg acggtcatca atcagtcgca tcagcaatat ctgcaaaaag acctgccgga
    13861 tttatatgtc gatctctcca ccgtcggtga aggttatgcg gcggatcacc tggcacgctt
    13921 gatggagcag gaagggattt cccgctatct ggtgtcggtg ggcggcgcgc tgaacagccg
    13981 tggtatgaac ggtgaaggcc agccgtggcg ggtagcgatt caaaaaccaa ccgataaaga
    14041 aaacgcggtt caggctgtgg tggatatcaa cggtcatggg attagcacct ctggcagcta
    14101 ccgtaactat tacgaactgg acggcaaacg tctttcccat gttatcgatc cgcaaaccgg
    14161 gcgtcccatc gaacacaatc tggtatccgt gacggtgatt gctccgacgg cgctggaagc
    14221 cgatgcctgg gatactggct tgatggtact cgggccggag aaagccaaag aggttgttcg
    14281 ccgggaaggg ctggcggttt atatgatcac caaagaaggc gatagcttta aaacctggat
    14341 gtcaccacag tttaaaagct tccttgtcag cgaaaaaaat taaagcgcaa gattgttggt
    14401 ttttgcgtga tggtgaccgg gcagactaaa ggctatcctt aaccagggag ctgattatga
    14461 aaaacgccac atgcttaact gacgatcaac gctggcaatc tgtcttagcc cgcgacccga
    14521 atgccgacgg cgaattcgtt ttcgccgtgc gcaccacggg tatcttttgc cgtccttctt
    14581 gccgcgccag acatgccttg cgggaaaatg tctccttcta cgcaaatgcc agcgaggcac
    14641 tcgccgccgg atttcgcccc tgcaaacgtt gtcagccaga caaagccaat ccccggcaac
    14701 atcggttgga taaaatcacc cacgcgtgtc gactgctgga acaggaaacg cctgtaacgc
    14761 tggaagcctt agccgaccag gtggcgatga gtccatttca tctacatcgg ttgtttaaag
    14821 ctactaccgg aatgacgcct aaagcctggc aacaggcctg gcgcgctcgc cgtttgcgcg
    14881 aatcgctggc gaaaggggag agcgtgacga cgtctattct taacgccgga ttccccgaca
    14941 gcagcagtta ctaccgcaaa gccgatgaaa cgctgggcat gacggctaaa caattccgtc
    15001 acggtggcga aaatctggcg gtgcgttacg cgctggctga ttgtgagctg ggtcgttgcc
    15061 tggtggcaga aagcgagcgg gggatttgcg cgatattgct gggcgatgat gacgcgacat
    15121 taatcagcga gttgcagcag atgtttcccg ctgccgacaa cgcgcctgcc gatctgacgt
    15181 ttcagcaaca tgtgcgtgaa gtgatcgcca gcctcaatca acgcgatacg ccgctgacgt
    15241 taccgctgga tattcgcggc actgcttttc agcaacaagt ctggcaggca ctgcgcacga
    15301 taccttgtgg tgaaaccgtc agttatcagc aactggctaa cgccatcggc aaaccgaaag
    15361 cggtacgggc cgttgccagc gcctgtgccg ccaacaagct ggctatcgta ataccctgtc
    15421 atcgggtggt ccgtggtgat ggcacacttt ccggttaccg ctggggcgtg tcgcgtaaag
    15481 cgcaactgct gcgccgcgaa gctgaaaatg aggagaggta atgttggatc tgtttgccga
    15541 tgctgaaccg tggcaagagc cactggcggc tggtgcggta attttacggc gttttgcttt
    15601 taatgctgcg gagcaactaa tccgcgatat taatgacgtt gccagccagt cgccgtttcg
    15661 ccagatggtc gctccaggcg gatataccat gtcggtggcg atgaccaact gtgggcatct
    15721 gggctggacg acccatcggc aaggttatct ctattcgccc attgatccgc aaacaaataa
    15781 accgtggccc gctatgccac aaagctttca tgatttatgt caacgtgcgg ctacggcggc
    15841 gggctatcca gatttccagc cagatgcttg tcttatcaac cgctacgctc ctggcgcgaa
    15901 actgtcgctg catcaggata aagacgaacc ggatctgcgc gcgccaattg tttctgtttc
    15961 tctgggctta cccgcgattt ttcaatttgg tggcctgaaa cgtaatgatc cgctcaaacg
    16021 tttgttgttg gaacatggcg atgtggtggt atggggcggt gaatcgcggc tgttttatca
    16081 cggtattcaa ccgttgaaag cggggtttca tccactcacc accgactgcc gctacaacct
    16141 gacattccgt caggcaggta aaaaagaata aaaataagaa ttattattgc tgtgcgcgcg
    16201 aagatgttta aactgcgggc tgtaattcat tgtccgggtt ttctgcatgg aacttcttgt
    16261 acttgtctgg cggcagtatc gctggccatt tatcagtgtg atggcgctaa gcctcgccag
    16321 tgcggcatta ggcattggct taattgcttt tatcaatcag cgccttatcg aaacggcgga
    16381 taccagtctg ctggtgttac cggagtttct gggattattg ctgctgttga tggcagtcac
    16441 tctcggatcg caactggcgc tcaccacttt ggggcatcac ttcgtttacc gactgcgtag
    16501 tgaatttatc aagcggattc tggataccca cgtcgagcgc attgaacaac tcggtagcgc
    16561 ctcgttgctg gcgggattaa ccagcgatgt gcgcaatatc accattgctt ttgtgcgtct
    16621 gccggaactg gggcagggga tcattctcac tatcggttca tcggcgtatc tgtggatgct
    16681 gtcgggcaaa atgttgctgg taacggctat ctggatggcg atcaccatct ggggcggttt
    16741 tgtgctggtg gcgcgggtgt acaaacatat ggcgacccta cgtgaaaccg aagacaagct
    16801 gtacacggat tttcaaactg tacttgaagg gcgcaaagag ctgactctga accgggaacg
    16861 cgccgagtat gtgtttaaca acctctacat ccccgatgcg caagagtatc gccatcatat
    16921 tattcgcgca gacaccttcc atcttagtgc cgtgaactgg tcaaacatca tgatgctggg
    16981 cgctatcggc ctggtgttct ggatggcgaa cagtctcggt tgggctgata ccaacgttgc
    17041 tgcgacctat tcgttgacgc tgttattcct gcgtacgccg ctgctttcgg cggttggcgc
    17101 attgccgacg ctgctgacgg cgcaggtggc gtttaacaag ctgaacaaat tcgcgctcgc
    17161 gcctttcaaa gctgagtttc cgcgcccgca ggcgtttccc aactggcaaa cgctggagct
    17221 gcgtaacgtg acgtttgctt atcaggataa cgcgttttcc gttggtccga ttaatctcac
    17281 catcaaacgt ggcgagctgc tgtttctgat tggcggtaac ggtagcggaa aatcgacgct
    17341 ggcgatgttg ttgacgggct tgtatcagcc acaaagcggc gaaatcttgc tggatggcaa
    17401 acctgtcagc ggcgaacaac cggaagatta tcgcaaactg ttttcggcag tgtttaccga
    17461 tgtctggctg tttgatcaac tgctggggcc ggaggggcaa cccgctaacc cgcaactggt
    17521 tgagaagtgg ctggcgcagc tgaaaatggc tcataagctt gagttaagca acgggcgtat
    17581 tgttaacctg aagttatcaa aagggcagaa aaaacgcgtg gcgctgttgc tggcgctggc
    17641 agaagaacgc gatattatcc tgctggatga atgggcggtg gatcaggatc cacacttccg
    17701 tcgtgagttt tatcaggtgt tgctgccgct gatgcaggag atgggtaaaa ctattttcgc
    17761 tatcagtcat gatgatcatt actttatcca cgccgaccgc ctgctggaaa tgcgcaatgg
    17821 gcaacttagc gagctgacgg gcgaagagcg cgatgccgct tcgcgtgatg ccgttgcccg
    17881 gacggcataa tattacgacg ctgacctggc ggactgctgc cgtcaggtca atatgctaaa
    17941 aatccct
//
LOCUS       DAZXUL010000087        17891 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZXUL010000087 DAZXUL010000000
VERSION     DAZXUL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17891
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.087"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            79..603
                     /locus_tag="ACH3L1_002885"
     CDS             79..603
                     /locus_tag="ACH3L1_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFT4077555.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            684..1358
                     /locus_tag="ACH3L1_002886"
     CDS             684..1358
                     /locus_tag="ACH3L1_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFT4077556.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1374..3836
                     /locus_tag="ACH3L1_002887"
     CDS             1374..3836
                     /locus_tag="ACH3L1_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFT4077557.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3852..4885
                     /locus_tag="ACH3L1_002888"
                     /pseudo
     CDS             3852..4885
                     /locus_tag="ACH3L1_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4967..5305)
                     /gene="rcnB"
                     /locus_tag="ACH3L1_002889"
     CDS             complement(4967..5305)
                     /gene="rcnB"
                     /locus_tag="ACH3L1_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFT4077558.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5524..6354)
                     /gene="rcnA"
                     /locus_tag="ACH3L1_002890"
                     /pseudo
     CDS             complement(5524..6354)
                     /gene="rcnA"
                     /locus_tag="ACH3L1_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6476..6748
                     /gene="rcnR"
                     /locus_tag="ACH3L1_002891"
     CDS             6476..6748
                     /gene="rcnR"
                     /locus_tag="ACH3L1_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFT4077559.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6840..6936
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6971..7759
                     /gene="thiM"
                     /locus_tag="ACH3L1_002892"
     CDS             6971..7759
                     /gene="thiM"
                     /locus_tag="ACH3L1_002892"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFT4077560.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7756..8556
                     /gene="thiD"
                     /locus_tag="ACH3L1_002893"
     CDS             7756..8556
                     /gene="thiD"
                     /locus_tag="ACH3L1_002893"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFT4077561.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8621..9438
                     /locus_tag="ACH3L1_002894"
                     /pseudo
     CDS             8621..9438
                     /locus_tag="ACH3L1_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            9490..10236
                     /locus_tag="ACH3L1_002895"
     CDS             9490..10236
                     /locus_tag="ACH3L1_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFT4077562.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10210..11175)
                     /locus_tag="ACH3L1_002896"
     CDS             complement(10210..11175)
                     /locus_tag="ACH3L1_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFT4077563.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11172..12175)
                     /locus_tag="ACH3L1_002897"
                     /pseudo
     CDS             complement(11172..12175)
                     /locus_tag="ACH3L1_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12215..13267
                     /gene="fbaB"
                     /locus_tag="ACH3L1_002898"
     CDS             12215..13267
                     /gene="fbaB"
                     /locus_tag="ACH3L1_002898"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFT4077564.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     CPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13575..14429
                     /gene="gatY"
                     /locus_tag="ACH3L1_002899"
     CDS             13575..14429
                     /gene="gatY"
                     /locus_tag="ACH3L1_002899"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFT4077565.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14458..15720
                     /gene="gatZ"
                     /locus_tag="ACH3L1_002900"
     CDS             14458..15720
                     /gene="gatZ"
                     /locus_tag="ACH3L1_002900"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFT4077566.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15730..16182
                     /gene="gatA"
                     /locus_tag="ACH3L1_002901"
     CDS             15730..16182
                     /gene="gatA"
                     /locus_tag="ACH3L1_002901"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFT4077567.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16213..16497
                     /gene="gatB"
                     /locus_tag="ACH3L1_002902"
     CDS             16213..16497
                     /gene="gatB"
                     /locus_tag="ACH3L1_002902"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFT4077568.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16501..17856
                     /locus_tag="ACH3L1_002903"
     CDS             16501..17856
                     /locus_tag="ACH3L1_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFT4077569.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4663 a         4093 c         4553 g         4582 t
ORIGIN      
        1 ttttttgatg tgagaaattt gtttccaaga gatacagact cttaacaacc aaaaataaat
       61 tttattaagg aacatattat gaaacgttca attattgctg ccgctgtctt ttcttctttt
      121 tttatgagcg ctggagtatt tgctgcagac gttgataccg gaacattaac cattaaagga
      181 aatattgcag aatctccgtg taaattcgaa gcgggtggtg attcagtaag tattaatatg
      241 ccgactgtac caaccactgt ctttgaaggt aaagctaaat attctaccta tgatgatgca
      301 gtcggtgtaa ccagcagcat gttaaaaatt agctgcccga aagaagttgc tggtgtaaaa
      361 ctctcgttga tcacaaacga caaaataacc ggtaacgata aggcgatagc cagtagcaac
      421 gataccgtag gtgataacag cgatgtccta gatgtttctg caccttttaa cattgagagt
      481 tataaaacag cggaaggtca atatgctatt ccgtttaaag caaaatacct gaaactgaca
      541 gataactcag tgcaatcagg tgatgtgtta tcttctctgg ttatgcgtgt ggcgcaggat
      601 taataaataa aattgcaagt ttctccaggt aataaacatg gccgctatcc ccatggcggc
      661 tttttaattt aagaggcatt aagatgaaag gattattatc tttactcatt ttttctatgg
      721 tccttcctgc acatgccgga attgttatct acgggacgcg cattatttac ccggcagaaa
      781 ataaagaagt tatggtgcag ttgatgaacc agggaaatcg ttcttcgctg ctgcaggcgt
      841 ggattgatga tggcgatacg tcgttaccac cagaaaaaat tcaggttcct ttcatgttaa
      901 cgccaccagt ggcaaaaata ggggcaaatt ccgggcagca agtaaaaatc aaaattatgc
      961 cgaataaact gcccactaat aaagaaagca ttttttatct gaatgttctg tatattccac
     1021 caaatagtcc agagcaagaa ggcaagaatg cactgaagtt tgcgatgcaa aacagaatta
     1081 agttgtttta ccggccagcg ggtattgctc cggtaaataa agcgaccttt aaaaagttac
     1141 tggtaaatcg cagtggcaat ggattggtga taaaaaatga ctcagctaat tgggtgacga
     1201 tttcggatgt caaagctaat aatgttaaag tcaattatga aactattatg attgccccct
     1261 tagaaagtca gagtgttaat atcaaaagta ataatgcaaa taactggtat ctgaccatta
     1321 tcaatgacca cggcaactat attagtgaca aaatttaatt gcaggagctg cctatgttga
     1381 gaatgacccc acttgcatca gcaatcgtag cgttattgct cggcattgaa gcttatgcag
     1441 ctgaagaaac ctttgatacc cattttatga taggtggaat gaaagaccag caggttgcaa
     1501 atattcgtct tgatgataat caacccttac cggggcagta tgacatcgat atttatgtca
     1561 ataagcaatg gcgcgggaaa tatgagatta ttgttaaaga caacccgcaa gaaacatgtt
     1621 tatcaagaga agttatcaag cggttaggca ttaatagcga taacttcgcc agcggtaagc
     1681 aatgtttaac atttgagcaa cttgttcagg gtgggagcta tagctgggat atcggggttt
     1741 ttcgtctcga tttcagtgtc ccgcaggctt gggtggaaga actggaaagt ggctatgttc
     1801 caccggaaaa ctgggagcgg ggtattaatg cgttttatac ttcttattat gtgagtcagt
     1861 attacagcga ctataaagcg tcgggtaaca acaagagtac atatgtacgt tttaacagcg
     1921 ggttaaattt actggagtgg caactgcatt ctgatgccag tttcagtaaa acaaataaca
     1981 atccaggggt gtggaaaagc aataccctgt atctggaacg tggatttgcc caatttctcg
     2041 gcacgcttcg cgtgggtgat atgtacacat caagcgatat ttttgattct gttcgcttca
     2101 gcggtgtgcg gttgtttcgt gatatgcaga tgttgcctaa ctcgaaacaa aattttacgc
     2161 cacgggtgca ggggattgct cagagtaacg cgctggtaac tattgaacag aatggttttg
     2221 tggtttatca gaaagaggtt cctcctggcc cgtttgcgat tacagatttg cagttggccg
     2281 gtggtggagc agatcttgat gttagcgtga aagaggcgga cggctcggta accacctatc
     2341 tggtgcctta tgcagcggtg ccaaatatgc tgcaacccgg cgtgtcgaaa tatgattttg
     2401 cggcgggggc gagcaagcaa agtgattttg tccaggcagg ttatcagtat ggttttaata
     2461 acttattgac gctgtatggc ggcacgatgg ttgctaataa ttactatgcg tttaccctcg
     2521 gaacgggttg gaacacgcgc attggcgcaa tttccgtcga tgccacgaag tcgcatagta
     2581 aacaagacaa cggcgatgtg tttgacgggc aaagttatca aattgcctac aacaaatttg
     2641 tgagccaaac gtcgacgcgt tttggtctgg cggcctggcg ttattcgtcg cgtgattacc
     2701 ggacatttaa cgatcacgtt tgggcaaaca ataaagataa ttatcgccgt gatgaaaacg
     2761 atatctatga cattgccgat tattaccaga acgattttgg ccgtaaaaat agcttttctg
     2821 ccaatatgag ccagtcattg ccagaaggct ggggttctgt gtccttaagt acgttatggc
     2881 gagattactg ggggcgtagc ggcagcagta aggattatca gttgagttat tccaataact
     2941 ggcggcggat aagctatacc ctcgcggcaa gccaggcgta tggcgagaat catcatgaag
     3001 agaaacgttt taatattttt atatcaattc cctgtgattg gggtgatgac gttacgacgc
     3061 ctcgtcggca aatatatatg tctaactcaa cgacgtttga tgatcagggt tttgcctcaa
     3121 ataatacggg attatcagga acagtaggga gtcgggatca gttcaattat ggtgtcaacc
     3181 tgagtcatca acatcaggga aatgaaacga cagctggggc gaatttgacc tggaacgcgc
     3241 cggttgcgac agtgaatggc agttatagtc agtcgagtac ttatcgacag actggagcca
     3301 gtgtttcagg gggcattgtc gcctggtcgg gtggcgttaa tctggcgaac cgtctttccg
     3361 aaacgtttgc tgtgatgaat gcgccaggaa ttaaagatgc ttatgtcaat gggcaaaaat
     3421 atcgcacaac aaaccgtaat ggagtggtgg tatacgacgg aatgacacct tatcgggaaa
     3481 atcacctgat gctggatgtg tcgcaaagcg atagcgaagc agaattacgt ggcaaccgga
     3541 aaattgccgc cccttatcgc ggcgcggttg tactggttaa ttttgatacc gatcagcgca
     3601 agccctggtt tataaaagcg ttaagagcgg atggtcaacc attaacgttt ggttatgaag
     3661 tcaatgatat ccatggtcat aatattggcg ttgtcggcca gggaagccag ttatttattc
     3721 gcaccaatga aataccgcca tcggttaatg tagcaattga taagcaacaa ggactttcat
     3781 gcacaatcac cttcggtaaa gagattgatg aaagtagaaa ttatatttgc cagtaatagt
     3841 caggtggttc tatggaaatt cgcataatgc tatttatatt aatgatgatg gttatgcctg
     3901 tgagctatgc ggcatgttat agtgagttat ctgttcagca caatttggtt gttcagggga
     3961 attttgcact tactcaaaca caaatagcga catatgagca taattttaat gattcgtcat
     4021 gcgtgagtac aaatactatc acccctatga gcccatcgga tattattgtt ggactttata
     4081 acgataccat taaattaaat ttacattttg aatggaccaa taaaaacaac atcacgttgt
     4141 caaataatca gagcagtttc accagtggtt attcggtaac ggtgacacct gcggccagta
     4201 atgcaaaagt gaatatttct gcggggggcg gtggttcagt gatgattaat ggtgttgcga
     4261 cattatccag tgcttcatca tcgacacgcg ggagtgccgc agtacaattt ctactgtgtt
     4321 tattaggtgg caagtcatgg ggtgcatgtg taaatagcta cagaaatgca ttggcacaaa
     4381 atgcaggtgt ctattccttc aatctgacat tgtcatacaa cccgataacc acaacctgca
     4441 aacaggacga tttattaatt actttagaca gtattcccgt tttacaatta ccagccacag
     4501 gtaacaaagc aacaataaat agtaaaaaaa gggatattat tctgcgttgt aaaaatttat
     4561 taggtcaaca aaatcaaaca tcacggaaaa tgcaggtgta tttatcaagt tctgacttgt
     4621 taaccaacag caacacaata ctgaaaggtg cggaagataa tgcgtaggat ttattcttga
     4681 aagtaatggt tcgccagtca cacttttaaa tatcactaac agcagtaaag gatacacaaa
     4741 tttaaaagaa attgcggcaa agtcaaaact tacagataca acggtttcaa ttccgataac
     4801 agccagttac tacgtctacg atacaaacaa agttaaatct ggcgcactgg aggcaaccgc
     4861 attaatcaac gtgaaatacg actaataaca agcaaaacga gcaagtggct aataataaaa
     4921 aataacaagg tgtctgcatt tccccctcat gatgaggggg ctttttttag cgatgataaa
     4981 aaatctcacc gtcgtaggct ttaatgattt taccgtcggt gtcgctgatc aacacgtacg
     5041 cgccacccat ataggtccag tgcgtcccgg catcaggcgc gggcaggtta cgtaactgcc
     5101 actgcttaat gttgtactca tcggtgcggt acatctctgg gacggtatca cctggtttga
     5161 aatgcttaaa gtcagcaatg aaactactta actcatactt agaaattccc gaggtattgg
     5221 tcgaacccgc ggtggctggt gcggcccagg cggcactggt aaccagcaaa agcacaccca
     5281 gcaacatctt atttttaatg gtcataaact ctccactttg gggatctgta atatcagtcg
     5341 catatctttc ctgtcataca atataactga caagcgatat tttgttagtg atcccatgat
     5401 ttcgtaaaaa gtgtaaatga aaggatgccc taaaagcatc cttagcgagc agagagtcag
     5461 cggaaatagc tgatttaaga atcatcctgg gctgaagata acatgcaagc ctaaaggatt
     5521 gagttatcgc attatgccca tgaagccatg tacgcccata tacacaccga ctaagccaat
     5581 caacagactg gaaaaatagg gggcgcgttt agcgagagtg ttaaatccgc tccagcgttt
     5641 tgcgacctgc tgaacgctga ttgctgcgcc aacgcctacg gtgacaagcg ttaacgccag
     5701 gccaatgctg aaactgacga ccagtgttgc gcccagtgtc agggctttca actgaatgca
     5761 tatcaacagc acggtaattg ctgccgggca ggggataaag ccaccggtta agccaaataa
     5821 caaaatttgc cagttgatga cctctctacc atcaaagcgt cgtttaatgt cattggcatg
     5881 ggctcgtgca tgggcatcct gatactcgcc atgttcgtga tggtgatgat gtccttggtc
     5941 gtggtgatgt tcgtgatcgt gatgatggtg atgatgctca taatcatgcc cgtgcatatt
     6001 ctccagccag ttgcgttcgc cgcgctaggt acgccagaac atccagaacg cggtgccaat
     6061 aatgatcact gcggaaatca gctggagcca cggttctgct gattgagcag taaagcgctt
     6121 actgatcacc atcccgccaa aggcaatcaa ccagaccacc gcggtatgcg aaatggttgc
     6181 tgccagtccg agcatcaccg cctgtttaat ggtgcctttg atggcgatga taaacgccgc
     6241 catcatcgtt tttgagtgcc ctggttccag gccatgaagc gcaccaagta agatggcgct
     6301 ggggatgaag aaccaggcgt ttccttgctg aagaagagtt gtaaattcgg tcatgataat
     6361 aattcttagt attaattcgg caatctgatt ctactccccc ccagtacctg atactacccc
     6421 cccagtagat taatagtgct atgatttttc atattcttgt taaccaggtg ttgccatgtc
     6481 tcatacaatc cgtgataaac agaaactgaa agcgcgtgcc agtaagattc agggccaggt
     6541 cgtggcgctc aagaaaatgc tcgacgagcc gcacgaatgc gctgcagttt tacaacagat
     6601 tgctgctatc cgtggagcgg taaacggtct gatgcgggaa gtgattaaag gtcatctgac
     6661 ggaacacatc gttcaccagg gggatgagct aaaacgtgaa gaagatctgg atgtcgttct
     6721 gaaggtgctg gattcatata tcaaataatt tattaacgcg attgtaaaac tgccgttttt
     6781 cctcgtttac aacgggggcg ctggacatta ccatcctcct ctgcgattta tcatcgcaac
     6841 caaacgactc ggggtgccct tctgcgtgaa ggctgagaaa tacccgtatc acctgatctg
     6901 gataatgcca gcgtagggaa gtcacggacc accaggtcat tgcttcttca cgttatggca
     6961 ggagcaaact atgcaagtcg acctgctgag ttcagcgcaa tctgcgcacg cgttacacct
     7021 ttttcaccaa cattcccctc ttgtgcactg catgaccaat gatgtggtgc aaacctttac
     7081 cgccaatacc ttgctggcgc tcggtgcatc gccagcgatg gttatcgaaa ccgaagaggc
     7141 cagtcagttt gcggctatcg ccagtgcctt gttgattaac gttggcacac tgacgcagcc
     7201 acgcgcacag gcgatgcgtg ctgccgttga gcaagcaaaa agctctcaaa cacgctggac
     7261 gcttgatcca gtagcggttg gtgcgctcga ttatcgccgc catttttgtc atgaactttt
     7321 atcttttaaa ccggcagcga tacgtggtaa tgcttcggaa atcatggcat tagctggcat
     7381 tgctaatggc ggacggggag tggataccac tgacgccgca gctaacgcga tacccgctgc
     7441 acaaacactg gcacgggaaa ctggcgcaat cgtcgtggtc actggcgagg tggattatgt
     7501 taccgatgga catcgtatca ttggtattca cggtggcgat ccgttaatga ccaaagtggt
     7561 aggaactggc tgtgcattat cggcggttgt cgctgcctgc tgtgcgttac caggcgatac
     7621 gctggaaaat gtcgcatctg cctgtcactg gatgaaacaa gccggagaac gcgcagtcgc
     7681 cagaagcgag gggccaggca gttttgttcc acatttcctt gatgcgctct ggcaattgac
     7741 gcaggaggtg caggcatgaa acgaattaac gctctgacga ttgccggtac tgatccgagt
     7801 ggtggtgcgg ggattcaggc cgatcttaaa accttctcgg cacttggcgc ttatggttgc
     7861 tcagttatta ctgcactggt ggcgcaaaat acccgtggcg tacagtcggt gtatcgcatt
     7921 gagcctgatt ttgtcgccgc ccagctcgat tcggtgttca gcgatgtgcg aatcgatacc
     7981 actaaaatcg gtatgttggc ggaaaccgat attgttgaag cggtggcaga acggttgcaa
     8041 cgttatcaga tccaaaacgt ggtactcgac accgttatgc tggcaaaaag cggcgacccg
     8101 ctgctttcac cttcggcggt tgctacgctg cgcagtcgat tattgccaca ggtttcatta
     8161 ataacgccaa acttgcccga agctgccgcc ttgctcgacg cgccacacgc gcgcaccgaa
     8221 caggaaatgc tggaacaagg gcgatcgctg ttggcgatgg gctgtggcgc agtgctaatg
     8281 aaaggtggtc atctggatga tgagcaaagc ccggactggc tgtttacccg cgagggtgaa
     8341 caacggttta ccgcaccgcg cattatgacc aaaaacaccc acggcactgg ttgtacactc
     8401 tctgcggcgt tggctgcact acgcccgcgc catacaaact gggctgacac cgtacaggag
     8461 gcaaaaatct ggctttcatc ggcgttagcc caggccgaca cgctggaagt tggtcacggt
     8521 attggtccgg ttcaccactt ccacgcctgg tggtgagcac cgtactatag ctgctttggt
     8581 tattgtcagc ctttggcact gacattcagg gatgaaacca atgcagctaa gaattaccag
     8641 tcgaaaaaaa ctcaccgctc tattatgcgc tcttgggcta atttccatcg ttgcaattta
     8701 tccgcgtcag acggtgaatt ttttctattc gacagcagtt cagattacag actacatcca
     8761 cttctacggt tatcgcccgg ttaaatcttt cgctattcgc attcctgcca gttacaccat
     8821 tcacggatta gatgtttcgc gctggcagga gcgtatcgac tggcagcggg tggcaaaaat
     8881 gcgcgacaac gatatccgct tacagtttgc ttttattaag gctacggaag gcgaaaagct
     8941 ggtggacccc tatttttcgc gtaactggca attaagccgc gaaaatggcc tgctgcgcgg
     9001 ggcgtatcat tatttctccc cgtcggtatc cgcttcagtt caggcgcgat tatttctgca
     9061 aacggtggat ttctcacaag gcgatttccc tgccgtgctg gacgtagaag aacggggaaa
     9121 attatcggca aaagaattac gcaagcgggt aagtcagtgg ctaaaaatgg tcgaaaaacg
     9181 cacgggaaaa aagccgatta tttactcagg agccgttttt tatcacacca atctggcggg
     9241 ctatttcaat gaatatccgt ggtgggtggc tcactattat caacgtcgtc cggacaatga
     9301 cggcatggcc tggcgcttct ggcagcattc cgaccgtgga caggtagatg gcatcaatgg
     9361 tccggtggat tttaatgtgt ttaatggcac ggggatgagt tgcaggcatt cgttgatggg
     9421 attaaagaaa cgccttaaat aagaagcagt gatatactcc caccagactc cctggcagga
     9481 aaaagacaaa tggaacaagc gcatacccag ctgattgccc aactgaacga gcgtatttta
     9541 gcggcggata acacgccgct ttatattaag tttgccgaaa cggtaaaaaa tgccgtgcgc
     9601 agcggggtgc tggagcatgg caatattttg cccggtgagc gtgatttaag tcagttaacc
     9661 ggcgtttcgc gcattacggt gcgcaaggcg atgcaggcgc tggaagaaga gggcgtggtg
     9721 acgcgttcgc gcggttacgg cacgcagatc aacaacatct tcgaatactc gttgaaagaa
     9781 gcgagaggtt tttctcagca ggtggtattg cgcgggaaaa agcccgatac gctatgggtt
     9841 aataagcgtg tcgtgaaatg tcccgaagaa gttgcgcagc agctggcggc cgaagcggga
     9901 agtgatgtct ttttgcttaa gcgtattcgc tatgtcgatg aagaagcggt atcgattgag
     9961 gaatcgtggg ttccggcgca tttaattcat gatgttgatg ccatcggaat ttctctttat
    10021 gactatttcc gcagccagca catttaccca caacgtacgc gttcccgcgt tagcgcccgg
    10081 atgccggatg ccgagtttca gtcacatatt cagttagata gcaaaatacc ggtgctggtg
    10141 atcaagcaag tggcgcttga ccaacagcaa cggcctattg agtacagcat tagccactgt
    10201 cgcagcgatc tatacgtttt tgtgtgcgag gagtagttcc tcgcgcgttg gcgcacaatc
    10261 accgccccga tgcccgacaa cccacgacgc cactgcattg cccagtaata cggcatccgc
    10321 cagtggcaga ccagaggcca gcccggcaag tacgccacct gcatgactgt cgcccgcccc
    10381 aatggtgtct acaacttgcg ttggaaatgc cggaatgcag ccagaagcgt cgttgctgaa
    10441 ataccatgcg ccttctttat cgagacgaac gatcaacggc gcggcaaatt tctcctgcca
    10501 ttgcttgcca agtgttgtta tctctgcgga taaagcaaaa cgttcggcgg caatctcagc
    10561 ctcttgacga ttgagtgaca ctaaaggtcg acaggccatg atccgcgcca gtaatgcgtc
    10621 cgggatatcg ccaatacgtg ggcaaaaatc gataaacggc gtcacgtctt gcagcttttc
    10681 cagccattcc actaacaatt cgccgcaggg cgaggccagt tgataaccgg aaaaatagag
    10741 caggttgcca ggcgcaacgg ttaatcgtgc caaccattgg cgattccatt gattttccac
    10801 accgctgaat gacataaaag tgcgttcgcc atccggctca accagtgcca gacaccagcc
    10861 gttatcgctt tcggcgttat cgatcaggct gattaacccc tctttcgcca tccggttgcg
    10921 aataatctcc gcccacacgc cctgaccgag cggcaaggca ttacccgctt cgatgccgag
    10981 gcgctttaac gccacggcaa tattcagtgc gcagccgcca acgttaacgc tctgctgttt
    11041 cagttcgata tcgcacccac gccaggggag ggcataagcg tcggcgatca cgtcaatgac
    11101 cgccgcgccg acgaccatca ccggctgacg cgtggttaat tcaggcagca gtgtgtgtaa
    11161 tcgagcgccg ctcataccgc ctcccgttgt tgacgatatt tcgccagcgc tgtggcatag
    11221 cggttgaaat caagctgatt taccgcatcc agctccgcct ttaacgcagg atcgacagcg
    11281 ttaatgccat gcaacgcgcc gcaaatcgcc gtcgccatag cacctatggt gtctgtgtcg
    11341 ccgccaagat tggcgcacag gacggcgcag cggtttgggt cggtttgtgc cagttcaact
    11401 agcgcaatgg cgcacggaac ggactcaata gtgctggtgc ctgcgccaac gacctggtaa
    11461 agctgttcgc tggcggattc ggtgccgtcg gcattacgca caattttcag cgcaatctcc
    11521 agacgtgccg ccagtgaggc gctgaacgtg gtggtgcgtt tttgctgtgc atgtcgcgca
    11581 attgaaggca atgaatcaac aatcgctgac cagcgttctc cgtcaatggc acgagaaatc
    11641 gcccatgcga tgactaccgc gcctgcaacc gccagatcgg atttatgggt cgggctggac
    11701 gccagcgcca catcatcaat aaaggaatca acatcacgcg ctggaagcaa acaacctaat
    11761 ggcgagacgc gcatcgctgc gccgttggtt acgccgttgt tttccagttc agcaacgggt
    11821 ttaccgtcgc gaatggcgtt aagcgcaatc ttcgaggtcg gacctagtac gtttttgtta
    11881 aaggcgtcga aacgcaacgc ccagtcgaga atattacgcc caatcagatt cggatcgatc
    11941 ttgccttcac attccagtaa cgcatccgcc agacacaggc catcgaggta tcgtcggtga
    12001 attcggcgcg gttaaaataa caggccgcgt tattctcctt tggtccaggc agaaaacggt
    12061 caatccagcc aaagtgtgct ttaacgcggc tgcgtggcca aagctcggag ggcatcccca
    12121 tcgcatcccc taacgcctgc ccataaagag caccgagaat acgttctgtt ttcatttaac
    12181 ttccccttga atcggaattt acgggagcat agtaatgaca gatattgcgc agttgcttgg
    12241 caaagacgcc gacaaccttt tacagcaccg ttgtatgact attccttctg accagcttta
    12301 tctccccgga catgactacg tagaccgcgt gatgattgac aataattgcc cgccagcggt
    12361 gttacgtaat atgcagacgt tgtacaacac cgggcgtctg gctggcacag gatatctttc
    12421 tattctgccg gttgaccagg gcgttgagca ctctgccgga gcttcatttg ctgctaaccc
    12481 gctctacttt gacccgaaaa acattgttga actggcgatc gaagcgggct gtaactgtgt
    12541 ggcatcaact tacggcgtgt tggtgtcggt atcgcggcgc tatgcgcatc gcattccatt
    12601 tctcgtcaaa cttaatcaca acgagacgct aagttacccg aatacctacg atcaaacgct
    12661 gtatgccagc gtggagcagg ccttcaacat gggcgcggtg gcggttggtg cgactatcta
    12721 ttttggctcg gaagagtcac gtcgccagat tgaagaaatt tctgcggcct ttgaacgcgc
    12781 gcacgagctg ggcatggtga cagtgctgtg ggcctatttg cgaaactccg cctttaagaa
    12841 agatggcgtt gattaccatg tttccgccga cctgactggt caggcgaacc atctggcggc
    12901 aaccataggt gcagatatcg tcaaacaaaa aatggcggaa aataacggcg gctataaagc
    12961 gattaattac ggttataccg acgatcgcgt gtacagcaag ttaaccagcg aaaacccgat
    13021 tgatctggtg cgttatcagt tagctaactg ctatatgggc cgggctgggt tgataaactc
    13081 cggcggtgct gcaggcggtg aaactgacct cagcgatgca gtgcgtactg cggttatcaa
    13141 caaacgcgca ggcggaatgg ggctgattct tggacgtaag gcgttcaaga aatcgatggc
    13201 tgacggcgtg aaactgatta atgccgtgca ggacgtttat ctcgatagca aaattactat
    13261 tgcctgatac gcctcatacg aacagcacca ccccgtagga cggataagac gtttacactg
    13321 catccggcaa cctatgcctg atgcgacgcg gaagcgtctt atcatgccta catagcactg
    13381 ccacgtatgt ttacaccgca tccggcataa aaacacgcgc actttgctac ggcttcccta
    13441 tcgggaggcc gtttttttgc ctttcactcc tcgaacaatt ttcatattgt tgtttttgcg
    13501 atcattatct cgacatttaa aagcaaataa tttcattata ttttgaaatc gaaagcaaac
    13561 gacaagatat gaaaatgtac gtggtatcga caaagcagat gctgaacaac gcacagcgcg
    13621 gcggttatgc ggttccggca ttcaatattc acaatctcga aacgatgcaa gtggtggtgg
    13681 aaaccgctgc caacctgcat gcgccggtca tcatcgccgg aacgcctggc acatttactc
    13741 atgctggtac agaaaatctg ttggcgctgg tcagcgcgat ggcgaagcaa tatcaccatc
    13801 cactggcaat tcatctcgac catcacacga aatttgacga tatcgctcag aaggttcgtt
    13861 ctggcgtgcg ctcagtcatg attgacgcct cgcatttgcc ttttgcgcaa aatatttcac
    13921 gggtcaaaga ggtggtggat ttttgccatc gctttgatgt cagcgtcgag gcggagctgg
    13981 ggcaacttgg cggccaggaa gatgatgtgc aagtcaatga agtcgatgcg ttgtacacca
    14041 accccgcaca ggcgcgtgaa tttgccgagg caaccgggat tgattccctg gcggtcgcca
    14101 tcggcacggc tcacggtatg tatgccagcg caccggtgct tgatttttct cgactggaga
    14161 acattcgcca gtgggtgaac ttaccgctgg tgctgcatgg cgcgtcagga ttatcgacta
    14221 aggatattca gcaaaccatc aaactgggga tatgcaaaat caacgttgca acggagctga
    14281 aaaatgcctt cttgcagtcg ttaaaaaatt acctgaccga gcaccccgaa gcgaccgatc
    14341 cccgggatta tttgcagtcg gctaaatccg caatgcgcga tgtggtgagc aaagtgattg
    14401 ccgattgtgg ctgcgagggc agggcataac gcacctgcca tttaacaagg aaaaaacatg
    14461 aaaacgttaa ttgcccggca taaagctggt gaacatatcg gcatatgttc agtctgttct
    14521 gcccatccgt tggttatcga agcggcgctg gcatttgatc gcaacagcac gcgcaaagtg
    14581 ctgattgaag caacgtcaaa ccaggtcaat caatttggcg gttataccgg aatgacaccg
    14641 gcagactttc gcgaatttgt ttttacgatt gccgataaag ttgggtttgc acgcgaacgc
    14701 attattctcg gcggcgatca tctgggacca aactgctggc agcaagaaaa tgcggatgcg
    14761 gcgatggaaa aatccgtcga gctggtaaaa gcatatgttc gtgctggctt cagtaaaatt
    14821 catcttgatg cgtcaatgtc ctgcgcgggg gataccatac cgttagcacc agaaacggtt
    14881 gcggaacgag ctgctgtgct ttgctttgct gcggaaagtg tggcgacaga ttgccagcgt
    14941 gagcaactga gctatgtcat tggcactgaa gttccggttc cgggcggtga ggccagcgcc
    15001 attcagtcag tacacatcac ccatgttgaa gatgccgcca atactttacg tacgcatcaa
    15061 aaggccttta ttgcccgtgg gctgacagag gcgttaacac gtgtgattgc catcgtggtg
    15121 cagccgggtg tggaatttga tcacagcaat attatccatt atcagccgca ggaagcgcag
    15181 gcgctggcgc aatggataga aaacacccga atggtttatg aagcacattc taccgattac
    15241 cagacccgga cggcttattg ggaattagtc cgcgatcact ttgcaatatt gaaagtcggt
    15301 cccgcattaa cctttgcttt acgcgaggcg atatttgcac tggcacaaat tgagcaggaa
    15361 cttatcgccc ccgaaaatcg cagcggttgc ctggcggtaa ttgaagaagt gatgctcgac
    15421 gaaccgcaat actggaaaaa atattatcgc acgggtttta acgattcatt actggatatt
    15481 cgttacagcc tgtcggatcg tattcgttat tactggccgc atagtcggat taaaaatagc
    15541 gttgaaacga tgatggtgaa tctgcaaggc gtggacatcc cactgggcat gattagtcag
    15601 tatcttccca aacaatttga acgcattcag tccggggaat tatcagcaat gccgcatcag
    15661 cttattatga ataaaattta tgatgttttg cgcgcctatc gctacggctg tgcggaataa
    15721 ggacggtata tgactaacct gtttgttcgt agcggaattt cttttgtcga tcgtagcgaa
    15781 gttttaacct atatcggtaa tgagatgctc gcgaaaggtg tggttcatga tacctggcca
    15841 caggcattaa ttgccagaga agcagaattc cctaccggga taatgcttga gcagcacgcc
    15901 attgcaatac cgcattgtga ggcgattcat gctaagtcgt cagccattta tctgttaagg
    15961 ccaacaaata aagttcattt tcagcaagcg gatgatgata acgacgtggc ggtatcgttg
    16021 gttattgcgt tgattgtgga aaatccgcag cagcaattga aacttttacg ctgtttattt
    16081 ggcaagttac aacagcccga tatcgtcgag acactaatta ctcttcctga aactcagtta
    16141 aaggaatact tcacaaagta tgttttagat tcagacgaat aaatccctct gtaacaataa
    16201 aaaggactat ttatgaaacg caagattatt gtcgcttgcg gaggcgcggt tgcgacctct
    16261 acgatggcgg cggaagaaat taaagagttg tgtcagagtc ataatattcc tgttgaatta
    16321 atccagtgtc gggttaatga aatagaaacc tatatggatg gtgtgcattt gatatgtacc
    16381 actgccaggg tggatcgtag ttttggcgat attccgttag ttcacggcat gccttttgtt
    16441 tctggtgtcg gtatcgaagc attacaaaat aaaattctga ctatcttaca gggatgacct
    16501 atgttttcag aagtcatgcg ttatattctc gacctcggcc ctacagtgat gctgccggtt
    16561 gtcatcatta ttttttctaa aatattaggc atgaaggcag gcgattgctt taaagcgggt
    16621 ctgcatatcg ggattggctt tgttggcatt ggccttgtga ttggcttaat gctggattcc
    16681 attggtccgg cggcgaaagc gatggcggaa aatttcgacc tgaatctgca tgtggtcgat
    16741 gtcggctggc cgggctcttc accaatgacc tgggcgtcgc aaattgcgct ggtggcgatt
    16801 ccgattgcga tattggttaa cgtggcgatg ttactgaccc gtatgacgcg ggtggtaaat
    16861 gttgatatct ggaatatctg gcatatgacc ttcaccggcg cgttgctgca cctggcaacc
    16921 ggttcatgga tgatagggat ggcgggtgtg gtaattcacg cggcgtttgt ttataagctc
    16981 ggcgactggt ttgcccgcga tacccgaaat ttctttgagc tggaaggtat tgctattccg
    17041 cacggtacgt cggcgtatat ggggccgatt gcggtgctgg tcgatgctat catcgagaaa
    17101 atcccaggcg ttaaccgaat taaatttagc gccgacgata ttcagcgcaa atttggccct
    17161 tttggcgaac ctgtcaccgt gggttttgtg atggggctga ttatcggcat cctcgcgggt
    17221 tacgatgtca aaggtgtatt gcagctggcg gtaaaaacgg cggcagttat gctgctgatg
    17281 ccacggatga ttaaacccat catggatggt ttaacgccca tcgctaagca ggcccgtagt
    17341 cgtttacagg cgaagttcgg cggtcaggag ttcctgattg gcctggatcc cgcattactg
    17401 ctggggcata cggcggtggt atcggcaagc ctgattttta ttccgctcac cattttaatt
    17461 gctgtttgtg ttccgggtaa tcaggtactg ccgtttggcg atcttgccac tatcggcttc
    17521 ttcgtggcga tggcggtcgc ggtgcatcgt ggaaatctgt tccgcacctt aatctcgggt
    17581 gtcatcatta tgagcatcac cctgtggatc gcgacgcaaa ctattggttt gcacacccaa
    17641 ccggcggcta atgctggggc gttaaaagcc gggggtatgg tggcttcaat ggatcagggc
    17701 ggttctccca ttacctggtt actgattcag gttttctccc cgcaaaatat tcccggtttc
    17761 attattatcg gcgcaattta tctgaccggt attttcatga cctggcgtag agcgcgtggt
    17821 tttactaaac aagagaaagc cgttctcgca gaataatttt tacctgaggg ggaggttatc
    17881 ccccttaaca a
//
LOCUS       DAZXUL010000088        17763 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZXUL010000088 DAZXUL010000000
VERSION     DAZXUL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17763
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.088"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            159..842
                     /locus_tag="ACH3L1_002904"
     CDS             159..842
                     /locus_tag="ACH3L1_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFT4077570.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            886..5548
                     /locus_tag="ACH3L1_002905"
                     /pseudo
     CDS             886..5548
                     /locus_tag="ACH3L1_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5916..7571
                     /gene="lldP"
                     /locus_tag="ACH3L1_002906"
     CDS             5916..7571
                     /gene="lldP"
                     /locus_tag="ACH3L1_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFT4077571.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYLLTWMIP"
     gene            7571..8347
                     /gene="lldR"
                     /locus_tag="ACH3L1_002907"
     CDS             7571..8347
                     /gene="lldR"
                     /locus_tag="ACH3L1_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFT4077572.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8344..9534
                     /gene="lldD"
                     /locus_tag="ACH3L1_002908"
     CDS             8344..9534
                     /gene="lldD"
                     /locus_tag="ACH3L1_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFT4077573.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9730..10203
                     /gene="trmL"
                     /locus_tag="ACH3L1_002909"
     CDS             9730..10203
                     /gene="trmL"
                     /locus_tag="ACH3L1_002909"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFT4077574.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10256..11077)
                     /gene="cysE"
                     /locus_tag="ACH3L1_002910"
     CDS             complement(10256..11077)
                     /gene="cysE"
                     /locus_tag="ACH3L1_002910"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFT4077575.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11157..12176)
                     /gene="gpsA"
                     /locus_tag="ACH3L1_002911"
     CDS             complement(11157..12176)
                     /gene="gpsA"
                     /locus_tag="ACH3L1_002911"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFT4077576.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12176..12643)
                     /gene="secB"
                     /locus_tag="ACH3L1_002912"
     CDS             complement(12176..12643)
                     /gene="secB"
                     /locus_tag="ACH3L1_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFT4077577.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12706..12957)
                     /gene="grxC"
                     /locus_tag="ACH3L1_002913"
     CDS             complement(12706..12957)
                     /gene="grxC"
                     /locus_tag="ACH3L1_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFT4077578.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13099..13530)
                     /locus_tag="ACH3L1_002914"
     CDS             complement(13099..13530)
                     /locus_tag="ACH3L1_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFT4077579.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13775..15319
                     /gene="gpmM"
                     /locus_tag="ACH3L1_002915"
                     /gene_synonym="pgmI"
     CDS             13775..15319
                     /gene="gpmM"
                     /locus_tag="ACH3L1_002915"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFT4077580.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15353..16612
                     /gene="envC"
                     /locus_tag="ACH3L1_002916"
     CDS             15353..16612
                     /gene="envC"
                     /locus_tag="ACH3L1_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFT4077581.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16616..17575
                     /locus_tag="ACH3L1_002917"
     CDS             16616..17575
                     /locus_tag="ACH3L1_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFT4077582.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17562..17763)
                     /locus_tag="ACH3L1_002918"
                     /pseudo
     CDS             complement(17562..17763)
                     /locus_tag="ACH3L1_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4443 a         4616 c         4646 g         4058 t
ORIGIN      
        1 cttttgaaac gaatgataca tttttatgat gaatgcttaa aacaatattt aatcgacaca
       61 catcactgtc attaatgtaa agcaacatag ctcaccaata atacaatgga caactctacc
      121 ggcattgagc gctggtcgga ggaaataagg atttttacat gaacttgaag aagatttttt
      181 tcagtgctgt cactgtttca gttctctgcg cattaacggg atgtgactac attgaagaag
      241 gcaagccaga aagtagttta ctgaaacaac aagaagaaca taataataaa attgtgctac
      301 ttgaaaaaca acaagcacag ctaaagagtc aactcgaaac tattcaaaaa caacaaactg
      361 gcataataaa cagtacaaag actttaactc acgtaattaa atccgttaaa gaccaacaaa
      421 atacttttat ttttaccgag tttaacccag caaaaacgaa gtatttcatc ctgaacaacg
      481 gttcggtggc tttagcgggt cgagtgttat ccattgacgc cacagagaat ggtagtgtta
      541 ttcatatctc actggtcaac ttattaagta cacctatctc aaacattggt tttaatgcga
      601 catggggtgg tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt
      661 tcaacacatc aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt
      721 tgaccctgaa gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg
      781 aaaatattca gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat
      841 aaatgcagtt agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt
      901 aaagttatct ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc
      961 cgtggcaaga aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg
     1021 ttgtcgtcgt ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg tgttgattac
     1081 ggtagtggat cagctggcga cggctgggtt gctataggca aaggggcgaa agcaaatact
     1141 tttatgaaca ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa
     1201 tatagctctg ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg
     1261 gttagtgcaa tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg
     1321 ggccaatact caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct
     1381 atgggggact cttccaaagc ggaaggagca aacgccattg ccctggggcg tagcagtgta
     1441 gctagcggta ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct
     1501 attgcgatag gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg
     1561 aaggcaaaag ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat
     1621 actatcgcat taggaaatgg cagccaggct ctggcagata atgcaatcgc cataggccag
     1681 ggcaacaaag ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc
     1741 ttaaacacca ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt
     1801 ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg
     1861 gatttagaca ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg
     1921 aaaaatggcg cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc
     1981 attagcgact cggtagcaaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg
     2041 tactgttaca gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc
     2101 gctcgctgta cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc
     2161 tgctcatggt actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat
     2221 ttcagcctcc agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt
     2281 cgaagccaac accaccaata tcaccaacct gacggattcc gttggtgacc ttcaggctga
     2341 tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc aggataccac
     2401 cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg atgctgttaa
     2461 cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca atatcgccaa
     2521 taacacttcc aatattgcca ctaacaccac caacatctct aacctgactg agacggtgac
     2581 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca cggcagctca
     2641 tggcaccgag accaccagca aaataaccaa cgttaaagat ggcgacctga cgactggcag
     2701 caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg cgacgaatac
     2761 caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg tgactaatct
     2821 tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag ctcatggcaa
     2881 caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa ccagttccga
     2941 tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct acttcggcgg
     3001 caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa tcggtgaaac
     3061 aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta gcacgtctct
     3121 cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac acggtactaa
     3181 tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact ccagttctga
     3241 cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg cgctgggggg
     3301 tggtgccgaa gtcaatgcag acggcaccat cactgcgccg acgtacacca ttgctaatgc
     3361 tgattacgat aatgtcggtg atgccctgaa tgctatcgat accactcctg acgacgctct
     3421 gctctgggat gcggacgccg gtgaaaatgg tgcatttagc gccgctcacg gaaaagataa
     3481 aactgccagt gtaatcacta acgtcgctaa cggtgcaatc tctgctgcca gcagcgacgc
     3541 gattaacggc tcacaactct ataccaccaa taagtacatc gctgatgcgc tgggtggtga
     3601 cgcagaagtc aacgctgacg gcaccatcac cgcaccgact tacaccattg cgaacgccga
     3661 gtacaacaac gtcggtgacg ccctggatgc gcttgatgat aacgccctgc tgtgggataa
     3721 gactgccaat ggcggtgctg gagcctacaa tgccagccat gacggtaaag ccagcatcat
     3781 cactaatgtc gctaatggca gtattagtga ggacagtacc gatgcagtga acggttctca
     3841 gttgaatgcg acgaatatga tgattgagca gaacacccaa attatcaatc agctcgctgg
     3901 taacaccgac gcaacctata tcgaagaaaa tggcgctggt atcaactacg ttcgtactaa
     3961 cgacaacgat ttagccttta acgatgcaag cgcctctggt gtcggcgcta cagctgtagg
     4021 ttataacgct gtcgcgtctg gtgccagcag cgtagccatt ggtcagaaca gcagcagcac
     4081 cgttgatacc ggtattgcgc tgggtagcag ctccgtttcc agccgtgtga ttgccaaagg
     4141 ttctcgtgac actagcgtaa cggaaaatgg cgtggtgatt ggttatgaca ccactgacgg
     4201 cgaactgcta ggtgcattgt caattggtga tgacggtaaa taccgccaaa tcatcaacgt
     4261 agctgatggt tcagaagccc atgacgccgt tacggttcgc cagttgcaga acgctattgg
     4321 agcggtcgcc actacgccaa ccaagtactt tcacgccaac tcaacggcag aagactcact
     4381 ggccgttggt gaagactcgc tggcaatggg tgcgaaaact gtcgttaatg gtaatgcagg
     4441 tattggtatc ggtttgaaca ctctggttct ggctgatgcg atcaacggca ttgctatcgg
     4501 cagtaacgca cgcgcaaacc atgcaaacag tatcgcaatg ggtaacggtt ctcagaccac
     4561 ccgtggtgca cagactggct acaccgccta caacatggac gcaccgcaga actctgtagg
     4621 tgagttttct gtcggtagtg aagacggtca acgtcagatc acaaacgtcg cagctggttc
     4681 agcggatacc gatgcggtta acgtgggtca gttgaaagtc actgatgagc gcgtagcgca
     4741 aaatacccag agcattacta acctgaacaa tcaggtcact aatctggata ctcgcgttac
     4801 taatatcgaa aacggtattg gcgacattgt caccaccggt agcaccaagt acttcaagac
     4861 caacaccgat ggcgtagatg ccaacgccca gggtaaagat agcgttgcta ttggttctgg
     4921 ttccattgct gccgctgaca acagcgtcgc actgggtacc ggttccgttg cagaggaaga
     4981 aaatacaatc tctgtaggtt cttctactaa ccaacgccgt atcaccaacg tagctgccag
     5041 tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa tcttctgaag caggcggagt
     5101 gcgctacgac accaaagctg atggttctat cgactatagc aatatcaccc tcggtggtgg
     5161 caacggcagt acgactcgta tcagcaacgt ctccgctggc gtcaacaaca acgacgcggt
     5221 gaactacgcg cagttgaagc aaagcgcgca ggaaacgaag caatacaccg atcagcggat
     5281 ggttgagatg gataacaaac tgtctaaaac tgaaagcaag ttgagcggtg gtatcgcttc
     5341 tgcaatggca atgaccggtc tgccgcaggc ttatacaccg ggtgccagca tggcttctat
     5401 tggtggcggt acttacaacg gtgaatcggc agttgcttta ggtgtatcga tggtgagcgc
     5461 caatggtcgt tgggtctaca aattacaagg tagtaccaat agccagggtg aatactccgc
     5521 cgcactcggt gccggtattc agtggtaatg atccattaag ttagtgtgac taagggcaaa
     5581 cgccaaattg cctgatgcgt tacgcgcatc aggcaagata aggcgctccg ggtcaacaat
     5641 ctaagggcaa ttctctgatg aggattgccc ttttctttac cagacatctc cccccacaag
     5701 aattggccct accaattctt cgcttatctg acctctggtt cacaattccc caattaaaac
     5761 tcacatcaat attgccaata cataacattt agttaaccat tcattgtcat tatccctaca
     5821 caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag caacagactc
     5881 attacacgat gtgcgtggac tctaggagac ctgcaatgaa tctctggcaa caaaactacg
     5941 atcccgccgg gaatatctgg ctttccagtc tgatagcatc gcttcccatc ctgtttttct
     6001 tctttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg acggtagcaa
     6061 tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg ctggcctcgg
     6121 tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg gatcattatt gcagcagtgt
     6181 tcgtctataa gatctcggtg aaaaccgggc agtttgacat cattcgctcg tctattcttt
     6241 cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg tttctgtttc ggcgctttcc
     6301 ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac cgccgcattg ctggtcggcc
     6361 tgggttttaa accgctgtac gccgccggac tgtgcctgat tgttaacacc gcgccagtgg
     6421 catttggcgc gatgggcatt ccaatcctgg ttgccggaca agtaacaggc atcgacagct
     6481 ttgagattgg ccagatggtg gggcgtcagc taccgtttat gaccattatc gtgctgttct
     6541 ggatcatggc gattatggac ggctggcgcg gtatcaaaga gacgtggcct gcggtcgtgg
     6601 ttgcaggcgg ctcgtttgcc atcgctcagt accttagctc taacttcatt gggccggagc
     6661 tgccggacat tatctcttcg ctggtatcac tgctctgcct gacgctgttc ctcaaacgct
     6721 ggcaaccagt tcgcgtattc cgcttcggtg acttgggggc gtcacaggtt gatatgacgc
     6781 tggcccacac cggttacacc gcgggtcagg tgttacgtgc ctggacaccg ttcctgttcc
     6841 tgaccgctac cgtaacactg tggagcatcc cgccgtttaa agccctgttc gcatcgggtg
     6901 gtgcgctgta tgagtgggtg atcaacattc cggtgccgta cctcaataaa ctggttgccc
     6961 gtatgccacc agtggtcagc gaggctacag cctatgccgc cgtgtttaag tttgactggt
     7021 tctctgccac cggcaccgcc attctgtttg ctgcactgct ctcgattgtc tggctgaaga
     7081 tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct gaaagaactg gctctaccca
     7141 tctactccat cggcatggtg ctggcgttcg cctttatctc gaactattcc ggactgtcat
     7201 caacactggc actggcactg gcgcacaccg gtcatgcatt caccttcttc tcgccgttcc
     7261 tcggctggct gggggtattc ctgaccgggt cggatacctc atctaacgcc ctgttcgccg
     7321 cgctgcaagc caccgcagca caacaaattg gcgtctctga tctgttgctg gttgccgcca
     7381 ataccaccgg tggcgtcacc ggtaagatga tctccccgca atctatcgct atcgcctgtg
     7441 cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc aaacacagcc
     7501 tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcttatctc ttaacgtgga
     7561 tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg atcgtgtgcg
     7621 ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg agcgccaact
     7681 ggcgatgcaa ctcggcgtat cacgtaattc actacgcgag gcgctggcaa aactggtgag
     7741 cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt attcgctggc gtcatgacac
     7801 atggtcggag caaaacatcg tccagccgct aaaaacgctg atggccgatg atccggatta
     7861 cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcat ggcatgcggc
     7921 aatgcgcgcc acacctggcg acaaagaaaa gattcagctt tgctttgaag caacgctaag
     7981 tgaagacccg gatatcgcct cacaagcgga cgttcgtttt catctggcga ttgccgaagc
     8041 ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc tgcaatcctc
     8101 agtgaagcat agccgtcagc ggatgtatct ggtgccaccg gttttttcac aactgaccga
     8161 acaacatcag gctgtcattg acgccatttt tgctggtgat gccgacgggg cgcgtaaagc
     8221 aatgatggcg caccttagtt ttgttcacac caccatgaaa cgattcgatg aagatcaggc
     8281 tcgccacgca cggattaccc gcctgcccgg tgagcataat gagcattcga gggagaaaaa
     8341 cgcatgatta tttccgcagc cagcgattat cgcgccgcag cgcaacgcat tctgccgccg
     8401 ttcctgttcc actatatgga tggtggtgca tattctgaat acacgctgcg ccgcaacgtg
     8461 gaagatttgt cagaagtggc actgcgccag cgtattctga aaaacatgtc cgacttaagc
     8521 ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tagcactggc accggtgggt
     8581 ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag ccaaagcggc ggacgcgcat
     8641 ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt cgcgccagcc
     8701 atcaagcgcc caatgtggtt ccagctttat gtactgcgcg atcgcggctt tatgcgtaac
     8761 gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg ttttcaccgt ggatatgccg
     8821 acaccgggcg cacgctaccg tgatgcgcat tcaggtatga gcggcccaaa tgcggcaatg
     8881 cgccgctact tgcaggcggt gacgcatccg caatgggcgt gggatgtggg cctgaacggt
     8941 cgtccacatg atttaggtaa tatctcagct tatctcggca aaccgaccgg actggaagat
     9001 tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga ccttgagtgg
     9061 atccgcgatt tctgggatgg cccgatggtg atcaaaggga tcctcgatcc ggaagatgcg
     9121 cgcgatgcag tacgttttgg tgctgatgga attgtggttt ctaaccacgg tggccgccag
     9181 ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta ttgcagatgc ggtgaaaggt
     9241 gatatcgcca ttctggcgga tagcggaatt cgtaacgggc ttgatgtcgt gcgtatgatt
     9301 gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct ggcaacagcg
     9361 ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa aagagatgaa agtggcgatg
     9421 acgctgactg gcgcgaaatc gatcagtgaa attacgcaag attcgctggt gcaggggctg
     9481 ggtaaagagt tgcctacggc actggctccg atggcgaaag ggaatgcggc atagtcgttt
     9541 gcgccccctc accctaaccc tctccctcag ggagagggga ccgttcggcg ctgtatgtac
     9601 tccctcactc tgaaacgaca ccgcactctt tttttctccc tcgcccctcc ggggagggcc
     9661 ggggtgaggg gaaaaggccg cacggttcac caattttctg ctatcctgcc cccgcactaa
     9721 gggggcagta tgctaaacat cgtactttac gaaccagaaa ttccgccaaa tactggcaac
     9781 atcatccgtc tttgcgctaa taccggcttt cgtctacata tcatcgaacc gatgggattt
     9841 gcctgggacg acaagcgcct gcgccgcgcg gggctggact atcacgagtt taccgccgtt
     9901 acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa atccccagcg cctgtttgcc
     9961 ctcaccacga aaggtacgcc tgctcacagc gccgtaagct atcaggatgg cgattatctg
    10021 atgttcggcc cggaaacacg cggcctgcca gcgagcattc ttgatgccct gcccgctgaa
    10081 caaaaaattc gcattccgat ggtgccggac agccgcagca tgaatctgtc caatgcggtg
    10141 tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc cgggagcgtt gttgagagat
    10201 tagttactgt atgccggata gatgattaca tcgcatccgg cacgatcaca ggacattaga
    10261 tcccatcccc atactcaaat gtatggttaa taccgttgaa atgctggtcc atatccattg
    10321 atggcttatc gctgtctggt ttaccgacaa tacgagccgg aacgccagcg gcggttgtat
    10381 gcggcggcac cggttgcagc accacggaac ctgcgccaat cttcgcgccg cgaccaactt
    10441 caatattgcc gaggattttc gcgcccgcgc caatcatcac accttcacga attttcgggt
    10501 gacggtcacc accagattta cccgtaccgc caagcgtcac agattgcaga atcgatacgt
    10561 cgttttcaat taccgccgtt tcacccacaa cgatgcccgt ggcgtggtcg agcataatgc
    10621 cacggccaat ttttgctgcc gggtgaatat cgacctggaa cgtcacagaa acctggtttt
    10681 gcagaaagat tgccaatgcg cgacgcccct gattccacaa ccagtgaccg atgcgatagg
    10741 cctgcaaggc atgaaaaccc ttcaggtata acaacggggt tgagtattta tcgactgccg
    10801 ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga ggcgatcatt tccgggtcag
    10861 cggcgtaggc ttcttcaacc acttcacgga tagcaatagc aggcataatt ggcgatgaca
    10921 gcttgttcgc cagcatgtag ctcagtgcac tgccaaggtt ttcgtgcttg agtagcgtcg
    10981 cgtggtaaaa actggccagc attggctcac agtccgccag cgttctggct tcggctttaa
    11041 tattgttcca gacaatttcc agttcttcac acgacattgc ttactccaca cgatgagata
    11101 atgaccggcc cgttctgcgc gggccgggtc atagcggtaa caaaggttcc ctggggttag
    11161 tgactgctgc gctcgtcctt gcgtgcacga cctaataatg tcaatgctgc ctcgcgcgcg
    11221 ttttttccgc aatataatac ttgataaatt tcctcggtta ttggcatttc aacgccgaag
    11281 cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc cttccaccac ctgaccaatc
    11341 ttctcctgcg cgctttgtac atccatgccc tgaccgagca tcataccaaa acggcggtta
    11401 cgcgactggt tgtcggtaca ggtaagcacc agatcgccca gccccgccat gcccataaag
    11461 gtggcaggat cggcacccag cgccgcacca agacgcgaca tttcagccag cccacgggtg
    11521 atcagcgccg tacgcgcatt cgcaccaaaa ccgataccgt cagacatccc cgcgccaatg
    11581 gcaatgacgt ttttcaccgc gccgccaagc tgcacgccaa tgaaatccgg gttgctgtaa
    11641 acgcggaaac ttttgccgca gtgcagcagc tgctggagat catcggcaaa ggtctgatcg
    11701 gtcgaggcca gcgaaatagc tgtcggtaaa cctgccgcca gttctttcgc aaacgttggg
    11761 ccagagataa ccgccagcgg aatttgatcg cctaaggcct cacgcgccac gtcctgtaac
    11821 agacgtccgg tttccgcttc cagccctttg gtcgcccaca ccagacgcgc atcaggacgc
    11881 atcagcggtt taatctggcg cagcacttca ccaaagacat ggctgggtac gacgacgaga
    11941 atattacgac tggctgccag cgcagtggcg agatcgcttt caagatggag cgtatcggga
    12001 aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc gttcaagcgt tgcgatatgt
    12061 tcagggtcat ggccccagag gacaacctcg tggccatttc ttgccagggt aatggcaaga
    12121 gcggtgccgt acgagccggc accgatcaca gtcattgaag cattacgttg gttcatcagg
    12181 catcctgatg ttcttcagta ccttcgccag cctgctgctg caaatagttc atgaacagcg
    12241 catcgaagtt aaccggcgca aggttcagtt gcgggaatgt accgcgggat accatgctgg
    12301 tgatgcactc acgagcatac gggaacagaa tgttcgggca gtatgcgccc aggcaatgtg
    12361 ccatctgggt gccttcgata cccgcgatgg agaaaatacc gccctgctga acttcacaca
    12421 ggaacgcggt ttcttcgccc aaggaggccg ttacggtaac acgcagtacc acttcgtata
    12481 cgtcatctgc cagttgggaa gatgccgtat ccagatcaag tttaacttct ggttgccaat
    12541 ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga gatatccttg gtataaatac
    12601 gttggatctg gaaagtcatt tcagtgttgt tttgttctga catgtgtaga aaacccttaa
    12661 gtgttgtcct taaatactgc gcaatgtaat accaacgttc acacgttatt tcagcagggg
    12721 atccagtcca ccacgtgcat ccaatgcata caagtcatca cagccgccaa tgtgctgtgc
    12781 gtcaataaaa atctggggaa ccgtggtgca accgctgcgt ttgatcatct cttcacgctt
    12841 ggcggcattg ccatcgatcg gcaactcctg gaaactcacg cccttgctgc tcagcagtgc
    12901 ttttgcacga tggcaatacg ggcaggtttc tttggtatag atttcaacat tggccataat
    12961 ttagctcctg tttttttacc ctgaaatttc atgttgcagg gcggctgcaa aatggagtgt
    13021 ccccagaagc ttataacaat aagtcgctgg gggcctaatt gcagctaacg gcctgcatca
    13081 tgaaagacga caggtaaatt atttgccgcg caccagaggc aagttttcgc cagcccagcc
    13141 agcaacgccc tctttcagca cgaatacttg cgcaaaacca gctttcgtca gcgcgtttgc
    13201 aggctcctgg cactgcatgc cagaaccgtc taccacgata accgatttgt ctttgtgctt
    13261 ctcaagctca ccaacattgt tggctttgat ttcgctcggc aacagattga tagaacctgc
    13321 gatatggcct ttacggaagt catcacgctg acgtaaatcc acaaccacag cgtcttcttt
    13381 gttgatcaga cgcgtagctt caccacgagt aatcaccttc actttcgagg tcaggctctt
    13441 aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag gcgatactca gtatgggatg
    13501 acggccaaca aattgcataa tttcttgcat ggggggtaac aactcccgac gtagtgatta
    13561 aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa tacagccagc gcgttaactg
    13621 gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa tctggcatcg gcttgccgcg
    13681 agcggtcgta tattttgatc ttcagaggct attttatcga ttcagctgta gtaaaattac
    13741 gcaaattttg actcttgagt atgaggttgt cgcaatgtcg gtttctaaaa aacctatggt
    13801 actggtgatt ctggatggct atggctatcg tgaagaacag caggataacg ccatttttag
    13861 tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt ccgcataccc tgatcgacgc
    13921 ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt aactccgaag taggccacgt
    13981 taacctgggt gccggccgca tcgtgtatca ggacctgact cgtctggacg ttgaaatcaa
    14041 agatcgcgct ttctttgcta atccagtgct ggctggcgcg gtacataaag cgaaaaacgc
    14101 aggtaaagca gtacacatta tgggtctgct ctccgcaggc ggcgtacaca gccacgaaga
    14161 tcacatcatg gcgatggtag aactggcagc tgaacgcggc gcagaaaaaa tctacctgca
    14221 cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct gaatcctcgc tgaaaaaatt
    14281 tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg tccatcattg gtcgttacta
    14341 cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa gcctatgacc tgctgactct
    14401 ggcgcagggt gagttccagg ccgatactgc cgttgctggt ttgcaggcag cttatgctcg
    14461 cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt gcagaaggtc agccagatgc
    14521 ggcaatggaa gacggcgatg cgctgatttt catgaacttc cgtgctgacc gcgcgcgtga
    14581 aatcactcgt gcattcgtga acgctgattt cgatggcttc gcgcgtaaga aagtggttaa
    14641 cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc aaaactgcgg ttgcttaccc
    14701 acccgcttcc ctggttaaca ccttcggcga gtggatggcg aaaaacgaca aaactcagtt
    14761 gcgtatttcc gaaaccgaaa aatatgccca cgttactttc ttcttcaacg gtggcgtaga
    14821 agagtcgttc aaaggcgaag atcgcattct gatcaactca ccgaaagtgg ctacctacga
    14881 tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa ctggttgcgg ccatcaagag
    14941 cggcaaatac gacaccatta tctgtaacta tccgaacggc gacatggtag gtcacaccgg
    15001 ggtgatggaa gcggcggtta aagcggttga agcactggat cactgcgtgg aagaagtcgc
    15061 gaaagcggtt gagtccgtgg gtggacaact gctgatcacc gctgaccacg gtaacgctga
    15121 acagatgcgc gatccggcaa cgggtcaggc acacacggca cacaccaacc tgccagttcc
    15181 gctgatttac gttggtgata agaacgtgaa agcggttgca ggcggcaaac tttctgacat
    15241 cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg caagagatga ctggtaagcc
    15301 gctgttcatc gtggaataat ccctccccat gaggggaaag gcgattaata ccatgacacg
    15361 ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc tacgccagcg ttcttagcgc
    15421 tggcgtattg ttgtgcgcct tttccgccca cgcggatgag cgtgaccaac tcaaatctat
    15481 tcaggccgat atcgccgcaa aagagcgcgc ggtacgccaa aagcaacaac aacgcgcaag
    15541 cctgctcgca caattgaaaa agcaggaaga agcgatctct gaagccaccc gtaagctgcg
    15601 cgaaacgcaa aacacgctca atcaactcaa taaacagatt gatgagatga acgcgtcgat
    15661 tgccaaactg gagcagcaaa aagccgccca ggagcgcagc ctcgccgcac aactggatgc
    15721 cgcattccgt cagggcgagc ataccggtat tcagctaatt ctcagcggtg aagaaagcca
    15781 gcgtggacag cgtttacagg cttatttcgg ctatctcaac caggcgcgac aagaaaccat
    15841 tgcccagttg aagcaaacgc gtgaagaagt caccatgcag catgctgaac tggaagagaa
    15901 acaaagcgag caacaaacgc tgttatatga acagcgcgcc caacaggcga aactgactca
    15961 ggctctgaac gagcgtaaaa agacgctggc ggggctggaa tcttccatcc agcaaggtca
    16021 gcagcagcta agtgaactgc gtgccaacga atcccgtctg cgtaacagca ttgcccgtgc
    16081 tgaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt gaggcccagg cggttcgcga
    16141 gcgccagaaa gaagcgacgc gcaaaggcac cacctacaaa ccgaccgaaa gcgaaaaatc
    16201 gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt caggcattct ggccggttcg
    16261 cgggccgacg ctgcatcgct atggcgagca gctacagggt gaattacgct ggaaagggat
    16321 ggttatcggt gcctctgaag gtactgaagt taaagcaatt gccgacggtc gagtgattct
    16381 ggctgactgg ctgcaaggtt acggtctggt ggtggtggtt gagcacggta aaggcgacat
    16441 gagtctttac ggctataatc agagcgcact ggtgagcgtt ggttcgcagg ttcgcgcggg
    16501 ccagccaatt gcactggtgg gcagcagtgg cggtcagggt cggccttcac tctatttcga
    16561 aattcgccgc cagggtcagg cggtcaatcc acagccgtgg ttgggaagat aagttttgtt
    16621 tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg gcgctctcct cccccgtact
    16681 tgctggcaaa cttgccatcg tcattgatga ttttgggtat cgcccgcaca acgaaaacca
    16741 ggtgctggcg atgccttccg ctatctccgt cgctgtatta cccgattcac cgcacgccag
    16801 agagatggcg accaaagcgc ataacagcgg tcacgaagtg ttgattcatc tcccgatggc
    16861 accgttgagt aaacagccgc tggagaaaaa tacgctacgc ccggagatga gcagcgacga
    16921 aattgagcgc attattcgta gtgcggtcaa taacgtgccc tatgccgtgg ggatcaacaa
    16981 ccacatgggc agcaagatga cctctaacct gtttggtatg cagaaagtga tgcaggcgct
    17041 ggagcgttac aatctttact tcctcgacag cgtaaccatc ggtaataccc aggcgatgcg
    17101 cgccgcgcaa ggtactggcg tgaaggtgat caaacggaag gtgttcctcg acgattcgca
    17161 gaacgaagcg gacatccgtg tgcaatttaa tcgcgcaatt gatctggcgc gtcgcaacgg
    17221 ttcaaccatt gccattgggc atcctcaccc ttcaacggta cgcgtgctac aacagatggt
    17281 ttataacctg ccgccagaca ttacgctggt gaaagccagc agcttgctga atgaaccgca
    17341 ggttgatact tctacaccgc cgaaaaacgc tgtgcctgat gcaccgcgta atccgttccg
    17401 tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct gtttatgcta atcgcttctt
    17461 tgaagtatta agcgaaagca tcagccagag cacgctgatc gtttacttcc agcatcagtg
    17521 gcaaggctgg ggcaaacagc ccgaagcggc gaagtttaac gctagcgcaa attaaaggcg
    17581 ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc attgccataa gcggaaagac
    17641 cacagtaacg cctggtaacc gaccttcacg ctgcgcacat tggtaatcag gcgcttatac
    17701 atacccggaa gtaaatatct cggcaatcat ccgttggcgg gtaagaatat ccggctcttt
    17761 gcg
//
LOCUS       DAZXUL010000089        17647 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZXUL010000089 DAZXUL010000000
VERSION     DAZXUL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17647
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.089"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..674)
                     /gene="chbR"
                     /locus_tag="ACH3L1_002919"
                     /pseudo
     CDS             complement(1..674)
                     /gene="chbR"
                     /locus_tag="ACH3L1_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(682..1032)
                     /gene="chbA"
                     /locus_tag="ACH3L1_002920"
     CDS             complement(682..1032)
                     /gene="chbA"
                     /locus_tag="ACH3L1_002920"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFT4077583.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1091..2439)
                     /gene="chbC"
                     /locus_tag="ACH3L1_002921"
                     /pseudo
     CDS             complement(1091..2439)
                     /gene="chbC"
                     /locus_tag="ACH3L1_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2524..2844)
                     /gene="chbB"
                     /locus_tag="ACH3L1_002922"
     CDS             complement(2524..2844)
                     /gene="chbB"
                     /locus_tag="ACH3L1_002922"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFT4077584.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3143..3481)
                     /gene="osmE"
                     /locus_tag="ACH3L1_002923"
     CDS             complement(3143..3481)
                     /gene="osmE"
                     /locus_tag="ACH3L1_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFT4077585.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3683..4510
                     /gene="nadE"
                     /locus_tag="ACH3L1_002924"
     CDS             3683..4510
                     /gene="nadE"
                     /locus_tag="ACH3L1_002924"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFT4077586.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4740..5627
                     /gene="cho"
                     /locus_tag="ACH3L1_002925"
     CDS             4740..5627
                     /gene="cho"
                     /locus_tag="ACH3L1_002925"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFT4077587.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5587..6162)
                     /gene="ves"
                     /locus_tag="ACH3L1_002926"
     CDS             complement(5587..6162)
                     /gene="ves"
                     /locus_tag="ACH3L1_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFT4077588.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6365..6850)
                     /gene="spy"
                     /locus_tag="ACH3L1_002927"
     CDS             complement(6365..6850)
                     /gene="spy"
                     /locus_tag="ACH3L1_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFT4077589.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7180..8148)
                     /gene="astE"
                     /locus_tag="ACH3L1_002928"
     CDS             complement(7180..8148)
                     /gene="astE"
                     /locus_tag="ACH3L1_002928"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFT4077590.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8141..9474)
                     /gene="astB"
                     /locus_tag="ACH3L1_002929"
                     /pseudo
     CDS             complement(8141..9474)
                     /gene="astB"
                     /locus_tag="ACH3L1_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9471..10949)
                     /gene="astD"
                     /locus_tag="ACH3L1_002930"
     CDS             complement(9471..10949)
                     /gene="astD"
                     /locus_tag="ACH3L1_002930"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFT4077591.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10946..11980)
                     /gene="astA"
                     /locus_tag="ACH3L1_002931"
     CDS             complement(10946..11980)
                     /gene="astA"
                     /locus_tag="ACH3L1_002931"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFT4077592.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11977..13197)
                     /gene="astC"
                     /locus_tag="ACH3L1_002932"
                     /pseudo
     CDS             complement(11977..13197)
                     /gene="astC"
                     /locus_tag="ACH3L1_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13643..14449
                     /gene="xthA"
                     /locus_tag="ACH3L1_002933"
     CDS             13643..14449
                     /gene="xthA"
                     /locus_tag="ACH3L1_002933"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFT4077593.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14616..15326
                     /locus_tag="ACH3L1_002934"
     CDS             14616..15326
                     /locus_tag="ACH3L1_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFT4077594.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15331..16008
                     /gene="ydjY"
                     /locus_tag="ACH3L1_002935"
     CDS             15331..16008
                     /gene="ydjY"
                     /locus_tag="ACH3L1_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFT4077595.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16023..16730
                     /locus_tag="ACH3L1_002936"
     CDS             16023..16730
                     /locus_tag="ACH3L1_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFT4077596.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16730..17278
                     /locus_tag="ACH3L1_002937"
     CDS             16730..17278
                     /locus_tag="ACH3L1_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFT4077597.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17288..17614
                     /locus_tag="ACH3L1_002938"
                     /pseudo
     CDS             17288..17614
                     /locus_tag="ACH3L1_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4366 a         4687 c         4308 g         4286 t
ORIGIN      
        1 atctcattaa taatctgcat tggtgttttg ccataatatc gttgagtcgc tcgcgtcaaa
       61 tattcctgtg atttggctga caacgtcacc atattctcca gcgccgattc actaaactgc
      121 tctttatcat gcatcttttc taccgtactt ttcagccact acggtacatc atcaatcacc
      181 tgttcttcgc ggtaatgacg taaacggtta atgacataaa aagtaaccat ctcaacaaac
      241 tcttccagcc ctgtttcgcg gaaatttaat gaagaaatca ctgtttccac ataggtgaga
      301 aaagcgttat tggtccggta tacctgcgaa gcaacaaagc aataaggcaa caacggcagg
      361 taatgctgct caaaaaagcg tttactgatc ccaacgttca atatgcgcgt ggcaccaaac
      421 tcataaaaac tttgatggtg cgaacctaac ggaataaaaa caaaatcgcc ccgttccagt
      481 aacacgcgct taccgttaat ctcctggaaa taacgcccgg ttaataccag agtaaattca
      541 taatagtcgt gctgatgcag tccgctgata ctctcagttt tgttatagat aaacacatgg
      601 aagtttttgc cattaaacaa ctgctgttct cgggcagtgg caatttccgg cgcgttaatc
      661 actggctgca tcatcgactc cttatgcctt cagtttttca tgaagctcaa ttaattcagt
      721 aatcagttca cgcgcaagca tggacgtcat taaatgatcc tgagcgtgga ccagcaccag
      781 actcactttc atcttacctt cgcccgcatc gccttcaatc agtttcgtct gtaccagatg
      841 cgcttcattc aatgccatcc gtgactgatc catcatggct ttcgctgcgg caaaatcgcc
      901 ctgcttcgcc tgtttcaatg cggcatacgc caggctacgc gcttgtccag agttgatgat
      961 aagccccatc accacttctt ccagctcttc agcttctgtt tgcgtatcag gaatattatc
     1021 gagatccatc atacatcgtt cctctctctt cttaccggca agattacccg taccggcatc
     1081 gattaaaatt tcaggggtta gcgatatctt cttcgctctc ttctttatca atcgcatttt
     1141 gtgctttgtt agccaccaca acaaagggca gataaattaa cgttgcgatg ccaaggttga
     1201 agagtgcgac cagcaatgcg gcgacgctac cgttggtgtt aaagaaggct cccagaccgg
     1261 ttggcatggt ccacggtgca atattggtca ccggaggaat gatgcccatg tagtacgctg
     1321 cgagggtgat tgccgccaga atcggttgta ccagtacaaa cgggataaac atcaccgggt
     1381 tcatgataat tggcagacca aacagaatcg gttcgttaat ctggaagatg ccggacggca
     1441 gcgccagttt tgccacctga cgataatcag cacggcgaga ggcgataaag atagccagga
     1501 tcaggcctaa agtcgcacca ctgcccccaa ggaaaataaa ggagtccagc atcggcttgg
     1561 cccagatatg gaaggtctta ccggctgcca gcgccgcttc gacggaacca tattgctgat
     1621 aggtcgcgat attttccagc gcccacggcg tcataatgcc gttgtccagt gcggtcagcg
     1681 ccagcgcgcc atgaataccg aagaaccaga gcagtggaac aaagatcaca taggcccagc
     1741 ccaccacgct acccaacgat gccagtgggg ttgagatggt atccataatg atctgatgga
     1801 agttggtgcc ccaggtattc aacgcccagg caataatccc catcacggaa agaataataa
     1861 agccgggaat taatgccgag aaagaacgcg atactgaagc aggtacgctg tcgggcagtt
     1921 taatgaccca attgcggcgg acaataaagg taaacatttc tgccaccacc aggccaataa
     1981 taatcccgga gatgatattc gccccaccta accagtttgc gccaaccgca taggcctcac
     2041 cgacactata tggggtgacg gtcataaatg ctgcaacgga taacaaccca gccgccagcg
     2101 catcgacttt acgctcttct gccagcgcca tgccaataaa gaacggtgcc attaaagaca
     2161 ttattcctaa tgttccgtta tatacgttgc cgccaatacc tttcagacca ttaagtgttt
     2221 caatggttga ggcgtcgagg cgaataccta aggaataaaa aacgacccct ccccaaagct
     2281 tagaaaaacg ttgttaatta atacaaacat ggcccccgca agggttaacg gcattaagcg
     2341 aataaagcca tttttgattg cattaacgtg tggctgcttt cctattttaa ctgcaaaagg
     2401 gaggagtacc ttttcaagcg atgcaataac attactcata gaaaaatacc cttaaaaacc
     2461 gcaatttaaa tattacggta ttgatttatg aaataactct ttgacgggaa aatttaaaat
     2521 aaattaattt gctgcggctt ttttaatcgc tgcaaccgca gccttaagca cgcctaaacc
     2581 atcgactttg ccataaagca gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg
     2641 ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat cggcattctg
     2701 acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa cttcatattt
     2761 ttctgcctgt gcgcgcattt ttgataccag taaagagata gacatgcccg cagaacaaaa
     2821 cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct gtcagccaga
     2881 cactccgcaa gccttgacct gcttccatgc tctgggtaac ttgcgaaacc aacatgactt
     2941 tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc tttgacctta
     3001 taaattgttt ctccttgacc tgtggttatg accctcttca catttcgggc aaataattcg
     3061 cgaagataat taagaactca ggccaaaaaa aaccggcgca atggccggtt tccgttgtta
     3121 ctcaagcttt cagacgaatt gattacttcg cagcctgtgg atcagtgtcg tattcagcac
     3181 aggtctgata accggagttg atgacatgtc cggtatcatc taacgcgaca aagtaggttt
     3241 ctgctttacc atcacgttga cccaggatgt aggtctggca agtaccgcga gcatggatca
     3301 tgctcacttc agacgaaggt ttacccgcaa tttgtgcaac ctgcgcccgg ctcatgcctt
     3361 ttttgacgtc tttcaccaca ggctgtacaa actggtcttt ggtacgatca taagctgtac
     3421 aacccgccag catggttaat accgccgctg cactcagaat tcctgccata ttcttgttca
     3481 tattccgtcc tcttgtttat cagcgtgtta gataagcctg gaatacattg ggcgcttttt
     3541 caagcccgtg aacgaaacgg ctccgctttc agaggattcc tgtatgacgt tttaaccacc
     3601 attcagcccg ctgtcgcttg tcgtttcagt agcaacgggt tagctttaag gaagttttgt
     3661 cttttctgtc tggaggggtt caatgacatt gcaacaacaa ataataaagg cgctgggcgc
     3721 aaaaccgcag attaatgctg aagaggaaat tcgtcgtagt gtcgattttc tgaaaagcta
     3781 cctgcaaact tatccgttca ttaaatcact ggtgctcggg atcagcggcg gtcaggactc
     3841 cacgcttgcc ggaaagctgt gccagatggc gattaatgag ctgcgccagg aaactcgcaa
     3901 cgaatcactg caatttattg ccgtacgcct gccctatggt gttcaggctg acgaacaaga
     3961 ttgccaggat gccattgcct ttattcaacc ggatcgcgta ttaaccgtta atatcaaggg
     4021 cgcggtattg gctagcgagc aggcattgcg ggaagcaggc attgaactga gcgattttgt
     4081 ccgtggcaat gaaaaagcgc gtgagcggat gaaagcacaa tatagcattg cgggtatgac
     4141 cagcggtgtc gtggtgggca ccgatcatgc agcagaagcc attaccggat tcttcactaa
     4201 atatggtgac ggcggtacgg atattaatcc gctgtatcgt ctcaacaaac gccagggtaa
     4261 acagttactg acggcattag gttgcccgga acacctttat aagaaagccc caactgccga
     4321 tcttgaagat gatcgccctt ctctgccaga tgaagtggca ctcggcgtga cctatgacaa
     4381 tatcgacgac tatctggaag ggaaaaacct acctgaacag gtcgccagaa caatagagaa
     4441 ctggtatctg aaaaccgaac ataaacgccg tccgccaatt aacgttttcg atgatttctg
     4501 gaaaaagtaa taatttgcac ctattggatt gtgcgaaaaa gagtaatttg ttcacgccgg
     4561 atgcggcgtg aacgccttat tctacctaca aaactatgca aattcaatac attgcaggag
     4621 tcgaataggc ctgacaggcg tagcacgtca gacggtgtaa cctttgtcat caacccgcct
     4681 cttttttaat cgcctcccgc ctgttacact ggatagataa ccagcattcg gagtcaacag
     4741 tggtacggcg tttaacttct ccgcggctcg aatttgaagc tgcggcaatt tatgaatatc
     4801 ccgaacattt acattcattc cttaatgact tacccacccg accaggggtg tatctgtttc
     4861 atggcgaaag tgacaccatg ccgctctata tcggcaaaag cgttaatatc cgcagtcggg
     4921 tgctttctca tttacgcacc ccggatgaag ccgccatgct acggcaatcc cggcgaatta
     4981 gctggatctg caccgcaggc gaaatcggcg ctctgctcct tgaagcgcga ttaatcaaag
     5041 aacaacagcc gctgtttaat aaacggttgc gccgcaatcg ccagctctgt gccctgcaat
     5101 taaatgaaaa gcgcgtcgat gtggtgtatg ccaaagaggt ggatttttca cgagccccca
     5161 acctgtttgg cctgtttgcc aataggcgcg cagctttgca agcattgcag agcatcgctg
     5221 atgaacaaaa actttgttat ggcctgttgg gactggaacc gttaagtcgc ggtcgtgcat
     5281 gttttcgttc agcgctaaaa cgttgcgccg gagcatgctg cggtaaagag agccatgagg
     5341 aacatgcgct acgcttgcgc cagtctctgg agcgtttgcg agtggtgtgt tggccttggc
     5401 aaggtgcggt ggcactgaaa gaacagcacc cggaaatgac tcaatatcat attattcaaa
     5461 actggctgtg gctgggggcg gttaattcgc tggaagaagc gacaacgtta attcggacac
     5521 ccgccgggtt tgatcacgac ggttataaaa ttctttgtaa gccgctgctt tccggtaact
     5581 atgaaattac tgaacttgat ccggcgaatg accagcgagc cagttgattt cactgaacaa
     5641 cagtttacct tgcggttgta aaagccgcag ggtgtgcctg ccatcgaacc agcacacgcc
     5701 ctggtcggta gtgagcagtt tgtcgcccag ttgccacgcc ccgttaatca caaacaccac
     5761 cccgccgcgt gagccaaagg tagtaaacgt acgttcggca attctgactt tcgccttaca
     5821 gacatccagg cgcgtcataa tattgaaatc catcgacatc tgccctgccg tcagtttcgc
     5881 ttttaccacc tggtccgccg taaaggcaaa aggctgtaac ggttttaagg tatggttaaa
     5941 gcggtctgcg ctttcaagga gcatctcgcc gccttccagc aacgtcacta tcctttccat
     6001 gccgggaaac aaagaaaact ccccattcgc cgcgatggat gcaatgctgg cacgccagta
     6061 aaaatcacgt tttgccggtg ggaaagtgca aatttcgcgc gtttcaccgg cagcgtttcg
     6121 ccacagattc accgacattt tacgcatatc aaagtattcc atactcgctc ccggcccgtt
     6181 cttttcattg ttatcgttca ggtaaccgtg gatggttatc ggcaactcgg gtgatttacg
     6241 ttagtggtga tcaggctttt tttacaactg tttgagaaga gaaaagaaaa ccgccgatcc
     6301 tgtccaccgc attactgcaa ggtagtggac aagaccggcg gtcttaagtt ttttggctga
     6361 aagattattc agcagttgca ggcattttac cttttgccgc tggacgttct gtcagacgct
     6421 tctcaaaatt agcattaaat tgctttttct gttccggcgt caggatgttg taaattttgt
     6481 tctgggtttc catgtgcgcc agcatgttag ctttgcgctg ttcttccatt tttgcgatct
     6541 gcgcttcagc ttttgcttta tcgaaggtat cgctggcaat gatgtcatgc attgcgcggc
     6601 gttcttccag cggcggacgt ttcatctggt cacgctggcc tttcatgatt tcgtggatct
     6661 gctgtttctg cgcgtcggtc aggttcaggt ctttgaacat catgtcctga tgcggaccga
     6721 acttgccttt gtggtgcatc atcggcttcg cgtcagccgg tgctgcggta gtggtgtctg
     6781 cggcatgggc caggttagcc gcgccaagag ccagggtaga ggcaacaaac agtgcagtta
     6841 atttacgcat attctatatc cttcctttca gttatttatt acggctttct taagtagcgt
     6901 gccgtgttga cgagattaac tttactgact ttagcgtcaa ttaatcagag caacggtaaa
     6961 acaatgaaag tgtaaaaaac acttttgcgc caattatgga gaaaaaaaga aaatttgatg
     7021 gagagtgatg agagaatatt acaacacgat gattttgcag agattatgaa gaactatacc
     7081 ggatgactgg tgataaataa agcaaataac caggattaat ctgtattaat ttataagaaa
     7141 gcaacttaat acccgcagaa tgatttctgc gggtaagtat tagcttattt tttcgagcat
     7201 taatcccgcg cgtaatccca acgctaccaa cggattaggg aataacacat actctacatc
     7261 atgggttacg gtaaaacgtt cctctccgtc ctgcgccagc aatgttcctt tctcaaacgg
     7321 cataaaattc agcgtgtcac ttgccatatg catttcgaag gacggcgagt ggcgagtaat
     7381 ttgcgaaacc acccgataac ggagcggcgg tgttctcacg ataccgacac tctcaccaga
     7441 tagcagcgca gcaattgcgc tggcagttac tgcaaactgg cgaagatcgt tttgcccaaa
     7501 gggcaacgct ttgccaagtt ccaacgtaca ggccagcgcg ccaaaatgtc tgacgctgaa
     7561 atgggtaaac gtaccaccag gctcctgatg gaacaccagc gcctccagcc ccgccgcacc
     7621 cagccacgtc agaaatttct cgtcccaggg aatgtcgcgt tgcggtaata caccgaactg
     7681 cggatgcaag gagccacgaa ttgcggtatg tagatcaagg tgccaacgca cagattcttt
     7741 gccctggtca taaaaatctt ccaggcactg ttccagttcg cgcgcccgac aggtttctcc
     7801 gctttcagca aatagctgcc aacgaccgcc aaacattcgg ttcatatcgc tatggcaata
     7861 acgtttccct tgcttcagcg caggaggatt cccgaggatc accagcaacc gccaacgtaa
     7921 cgggatctcg ccgtgagata tcgcgccaag caacgcgtcc agcatttcca caggtgccgt
     7981 ctcattaccg tggatccccg cagaaatcac cagcgcgcct tgcggtggcg ttaatggcgt
     8041 cagttccagc acaccatcgc ccagccagcg ccagcgaacg ccgttgattt ctcgctcggt
     8101 gataaccggt tttttacccg ttaaggtcag agcaagaaaa ttatccattg ccccctccct
     8161 cgcgctggaa cggataaacc gaaccgagat tcagtaattg gctcaataca tccagtgctt
     8221 cccgcccttc gcgcagcaat tgcgggtcgg ccagatcggc agcagtaaga cgatcgcggt
     8281 agtaacgatc cacccagtca ttgagcgcat taaacaaggt atcgttcatc atcaccgccg
     8341 gattcaccgc ccggcgttct tcttctgtca ataccacccg caaccgcagg cacgccgggc
     8401 cgccgccatt cgccatgctt tcacgtaagt caaagacttt tagttcgcta atcgggttgt
     8461 cagcggcaag gagttcattg agataacccc atactccggc gtgttcccga cactcctgag
     8521 gcagcaccaa catcatggaa ccatcatcgc ggctcaacag ttggctgtta aacagatagg
     8581 tagacaccgc atccgacacg gaaacctgag ttgccggaac ttctatcgcc ataaaaccgt
     8641 tcacccgcgt gccagtaact gtgactggcg agcgaacgcc tgttggtggc aaaacagcac
     8701 ctggcggtta ctcacggcaa tcacgtcatt atgaaaaacg ctctggtcga taacgtccgg
     8761 gttttgctgg gcgaaaatca cctgttgggg attcacctga ttaagccttg ccaccgcctc
     8821 gctggcttcg cgagtctgtc gcgccggata acgggaaggc cgggtatcat tgccttcttc
     8881 tcgcccgtag acaaaaagta gcatacccgg ttcaccgtaa tgaccgccga gacgattgtg
     8941 gtttgccgcc ccctcatcac cgagcaacgc tacctgcggc aacgccgaat ggacgctaaa
     9001 tttctcttcg tccttaaaaa tcgcttttaa cagcgattca gtgacgggcg cttccagcga
     9061 acggtgaaat ttattgttca ggttggcaac ggtgagatgc actttgccat ccagcgtatc
     9121 ggcagatggc gcgatcgttg ccgcattggc tacccacatt ggcgaagcgg aactgacgct
     9181 ggaaagccag tgcggtgcct ggcgtgcaac tttttccagt acctgctcat cgctaccact
     9241 gaatcccaac tgacgcagca ccggaataaa cggacgctcg tgcggcggaa tcacggcctg
     9301 ggggaatccc gcatcggcaa gggctttcat tttcagtaag ccctgttttg ccgccagtcg
     9361 cgggttagac acctgaaaac ggtgacgggt agaggcttca ttaccaaacg acaggcccgc
     9421 gtaatgatgc gtcagcccta ccagcccgtc gaaattaact tcccaggcgt tcatcgcacc
     9481 acctcatcgg aaaaatccag cccggggtta agcgtggcgg gcaatgttaa cgagtccgac
     9541 tccaggctcg ccataggcca tgcgcagtaa tctgcggcat accaggcgct ggggcgatgg
     9601 ttaccggaag caccaatgcc gccgaatggg gcggtactgg cagcaccagt aagcggtttg
     9661 ttccagttaa caatccccgc ccgcgcctcc agcaacagtt gatcgaattt ttcccgctcg
     9721 ggggaaacca gaccgcaaaa gagtccgaag cgagtgttgt tcgccatccg aatcgcctca
     9781 tcaaaagtat cataacgcca gacgcgcaaa aacggcccga acacctcttc atccggcaca
     9841 ccgccaacgc ccgtcatttc aatgatccct ggtgtcagca acgatgtccc tgcttgtaat
     9901 aagcgcggcg caagcagggt tcgtccgccc atcgcttcca gttgctgcca ggcagtaacc
     9961 acctgctgtg cggcctgttc agaaatcagc ccgccaataa acggctgcgg ttcgtcatcc
    10021 cagttgcccg gcgttaatcg ctggctgacg gcaaccagac tagcaagaaa cgcatcgccc
    10081 tgcgctccgc ttttgagcaa taaacggcgg gcgcaggtgc agcgttgccc ggctgtgaca
    10141 aacgccgact gaatggtcag atggacagcc gcgtcgatat ccgccacctc atcgataatt
    10201 agcggattat taccgcccat ctcaagggcg agaattttct ccggctgacc ggagagctgg
    10261 cgatgcagct ggtagccggt attggcgcta ccggtaaaca gcaaaccgtc gagatcctcc
    10321 agcgcactca gcgcctgacc cgtttcacgc ccgccctgca ccaggttcag cacgcccggc
    10381 ggcaagccag cctgctgcca taaacgcatt accgcttcgc cactccacgg tgtcagttcg
    10441 ctgggtttaa agataatggt gttacctgcc agcaatgccg gaacgatatg tccgttcggc
    10501 aaatgaccag ggaaattata cggcccaaac accgccagca cgccgtgcgg gcgatgtcgc
    10561 aggctcgccg caccgtccgg catttcacta cgctgctcgc cggtacgaac gtgatacgcc
    10621 ttaattgata tcgcgatttt attgatcatc gccgtcactt cggttgccgc ttcccagcgc
    10681 ggcttacccg tttctctggc aataatcgcg gttaattcgg ctttattgct ttccagcaat
    10741 ccggcaaagc gttcgacaac gacctgacgt tcagccaatg agagccgcgc ccagcgcgga
    10801 aacgccgcac gggctgcccg acaagcctgt tcgacctgag cggcatcggc atcattgcct
    10861 tgccataaca cctcgcccga taccggatta cgcttcacac gcgatgcgcc ctggcccgtt
    10921 atccagtcac cgttaatcca taaagtcatg ctgttttctc ctctgcgcac aggcgcacca
    10981 gacgaacgcg atcccctgcg tggcatttga gggcatccag ttgtgcggcg gttaaaatca
    11041 aacgctcggt tgccggatcg gtacgcgcca gcaccacgcg gaaatggtga taattttcat
    11101 tggcgaccag gcaggccgga aaatcgccct gcgcaggctg cccttctgcc acttccacca
    11161 gccgactttt acggatggcg cgcacgcggt cgatgtcaca ctcaagcgtt ggcccgccgt
    11221 cgaagatgtc gatatagttg cggtaacgaa aaccttcttt ctccagcacc gcgcgggcag
    11281 gcgcggtttg cggatgtacc tgaccgatga cgtcctgggc ttcctgggat aaaaagtggg
    11341 tatagatcgg atgtttcggc atcagttctg caataaatgc cttttgcccg gtgccgcaga
    11401 gaaaatcggc gcggctaaaa tccatcgaaa agaagcgttt accgaggctt tgccagaacg
    11461 gtgaatagcc gtgttcgtca atcaccccgc gcatttcagc aaccactttg tcattaaact
    11521 tgtcgcgaaa agccgccata aacataaagc gcgatttcga cagcaaatag ccgttgccct
    11581 ctttgcgcca gtccgggtcg agaaacagcg tgcacagctc gctgctgccg gtgtgatcgt
    11641 tactaagaaa cagcgtcggc aatgcgttat agacattcag ctcttttgag gcgtgaacca
    11701 acgtgccgac gcgatagttg taccagggat cgttcagccc aaccgccacc tcaatagcgc
    11761 aaatccccgc cacggtgcct gtctcgctat cttccagcac gaacacatag ccctgctcgc
    11821 ttttgggcag ttcaccttgc caggttttga ttgccctttc gatacgcgcc gaaagcgtgg
    11881 cttcattggc gggaagcgac gtcaggccgc cgcccgtttt gctggcaagc tgcatcagcg
    11941 ccgagacatc tgagcgctca acgggacgga tgaccatcat gatgaacctc ggctaacaaa
    12001 gtgttcgcaa gcagctgcaa agcgatccag tccggtcgtc acctcttctt cgctgacatt
    12061 gagcgcaggc gcaaaacgca ccacgttgcc acccgcaatc agtaccatca cgcctgcttt
    12121 cgccgcttcc agagagatct gtttcgcttg cccggcgtaa tcggcattca gtacacagcc
    12181 aatcaacaaa cctaagccgc gaacttcact gaacaaacca tagcggtgat taatagtatt
    12241 aagacgctca acaaaccagt cgtgacgctg tttaacgcca ttaagcatct ctggtgtgtt
    12301 gatgagctcc agcactttgc ctgctaccgc cgaggccagc ggattaccgc cataggtggt
    12361 gccatgagtg ccaacggtca tcacgcgggc gcactcttcg gttgccaaca acgcaccgac
    12421 cgggaaaccg ccgcccagcg ctttggcggt ggttaacaga tcgggcgtca cgccgtagtg
    12481 catataggca tacagctcac cagtgcgccc gacgccggtt tgtacctcat caaaaatcag
    12541 caaggcattg tggcggtcac acaattcacg cagaccttgt aaaaacgcgt tgctggctgg
    12601 caccacaccg ccttcgccct agatgggctc gacaatcacc gcacaggtgg cgtcgtcaat
    12661 cagcgcgctg gcagagttaa tatcgttata tgcagcatga cgaatatccg ccggcagtgg
    12721 cgcaaaatcc tgtgaatagg ctggctgccc acccgcactg acagtaaaca gcgtgcgacc
    12781 atgaaacgca tttttgaacg ccacgatgcc actcttatgg ctgccgtagc ggtcgtgagc
    12841 gaatttacgc gccagtttta gcgccgcttc gttggcttcc gcaccggagt tacaaaagaa
    12901 gacgcgatcg gcaaacgtgg cgtcgatcaa ttttttcgcc agtcgcagta ccggctcgtt
    12961 ggtgtaaccg ttgccggtat gccagaactt actcgcctgt tcgttcagcg cttcacgcag
    13021 ttccggatgc gcatggccca gcgcgttcac cgcaatgcca cccgcgaagt cgatatactc
    13081 tttcccctgc tgatcccaca agcgcgaacc ttcgccacgt accggtataa agggtgccgg
    13141 agcgtaaaca ggtatcatcc attcatcaaa gttttcacgc gtaattggct gagacatagc
    13201 gacctctaca gtaaataatt cgttatttgt atgttaacaa taagtaatgt ttgcgctgta
    13261 aatgtagatt gcagggttcg tgccagccag tgataaaagt gcataaacgg cggaggctaa
    13321 ctggaaatca aggagttata accaaaccat atgcatttaa agtgcatata aagtgaatac
    13381 gtttgcgatg tgggtgaata aaaagaataa aaaacgcaat gttatgcaga agtaaaatat
    13441 aattctagaa ttgtgatcat tgacgaaatt tactggaaat tactgcgcca ttctgacgca
    13501 gcgcgcacca aaagcgggca ttttttgcgc catcgttgac atcattaaca accatcgatc
    13561 aaatcactta acaacaggcg gtaagcaacg cgaaattctg ctaccatcca cgcactcttt
    13621 atctgaataa atggcagcga ctatgaaatt tgtctctttt aatatcaacg gcctgcgcgc
    13681 cagacctcac cagcttgaag ccatcgtcga aaagcaccaa ccggatgtga ttggcctgca
    13741 ggagacaaaa gttcatgacg atatgtttcc gctcgaagag gtggcgaagc tcggctacaa
    13801 cgtgttttat cacgggcaga aaggccatta tggcgtggcg ctgctgacca aagagacgcc
    13861 gattgccgtg cgtcgcggct ttcccggtga cgacgaagag gcgcagcggc ggattattat
    13921 ggcggaaatc ccctcactgc tgggtaatgt caccgtgatc aacggttact tcccgcaggg
    13981 tgaaagccgc gaccatccga taaaattccc ggcaaaagcg cagttttatc agaatctgca
    14041 aaactacctg gaaaccgaac tcaaacgtga taatccggta ctgattatgg gcgatatgaa
    14101 tatcagccct acagatctgg atatcggcat tggcgaagaa aaccgtaagc gctggctgcg
    14161 taccggtaaa tgctctttcc tgccggaaga gcgcgaatgg atggacaggc tgatgagctg
    14221 gggggtggtc gataccttcc gccatgctaa tccgcaaaca gcagatcgtt tctcatggtt
    14281 tgattaccgc tcaaaaggtt ttgacgataa ccgtggtctg cgcatcgacc tgctgctcgc
    14341 cagccaaccg ctggcagaat gttgcgtaga aaccggcatc gactatgaaa ttcgcagcat
    14401 ggaaaaaccg tccgatcacg cccccgtctg ggcgaccttc cgccgctaat ttagcagctc
    14461 tcctggctca aactgggtca ggagaattaa ccttgagaaa aatcaacaaa ctgtcagtaa
    14521 tgatttgttg cctgccgtcc tttgttatac cgtcactgcg tttttagttg tctgaccact
    14581 tctctattat caagtttgat ataggaaact ccacgatgaa cgctgagcgt aactttctgt
    14641 ttgcctgtct tatttttgcg ctggtcattt acgctatcca cgctttcggt ttattcgatc
    14701 tgctcaccga tttaccccac ttacagacac tcattcgcca gagcggattg ttcggttata
    14761 gcctctatat tctgttattc atcaatgccc ccctctttct gttaccagga agcatactgg
    14821 tgatcgctgg aggaatagtt tttggcccgc tcttagggac acaactctca ttaattgccg
    14881 caacgctggc ctcctcgtgc tcattcctgc tggcgcgctg gctgggacgt gatttactgc
    14941 tgaaatacgt gggccatagc cataccttcc aggccattga aaaaggcatt gcgcgtaacg
    15001 gtattgattt tcttattctg acccgcttaa tcccgttgtt tccttacaat attcaaaatt
    15061 acgcttacgg attcaccacc atcgccttct ggccttatac ccttatttcg gcactcacga
    15121 ccctgcccgg tattgttatt tataccgtga tggcaagcga tctcgccaat gaaggcatta
    15181 cgctgcgctt tattttacaa ctctgtctgg cgggcctggc gctgtttatt ctcatccagc
    15241 tcgcaaaact ctacgcccga cacaaacatg tggatctgtc tgcttcgcgc cgcagcccac
    15301 ttactcaccc aaaaaatgaa ggatagaacg atgtcgcaac attattcagt gtcatggaaa
    15361 aaaggactgg ctgcactctg cttactggct gttgccgggc ttagcggctg cgatcaaaaa
    15421 gagaatgcgg cggcaaaagt ggaatacgac ggactttcga acagccaacc gctgcgcgtc
    15481 gatgccaata accatacggt aaccatgctg gtgcaaatta atggtcgttt cctcaccgac
    15541 gacactcgtc acggtattgt gtttaaagat ggctccaacg gacataaatc gctgtttatg
    15601 gcttatgcgc ccccgaaagc attttatgaa gccctgaaag aggcaggtgg tacgccgggc
    15661 gaaaacatga cgatggataa taaagaaacg actcatgtca caggcagcaa actggatatt
    15721 tcggtcaact ggcaaggggc ggcaaaagcg tattccttcg atgaagtgat tgttgacagt
    15781 aatggcaaga aactggacat gcgctttggc ggtaatttaa cggcagcaga agagaagaaa
    15841 acgggttgcc tggtgtgtct ggatagctgc ccggtcggca tcgtcagcaa tgcaacatac
    15901 acttatggtg cggtggaaaa acacggcgaa gtgaaattca aaggcaatgc ctcagttctc
    15961 ccggcggata acacgctggc aacggttacc tttaaaatca ccgaataaag ccaagataaa
    16021 ggatgatgaa gatacaatcg cgtaaaatct ggtactaccg tataaccctc atcatcctgt
    16081 tgttcgctat gctgctcgca tgggcgctgc ttcccggcgt ccatgagttt atcaatcgca
    16141 gcgttgcggc gtttgccgcc gtggaccaac agggtataga acgctttatt cagtcttacg
    16201 gcgcactggc ggcagttgtc tcgttcttgt tgatgatttt acaggccatt gctgcaccgc
    16261 tacctgcgtt tttgatcacc tttgccaatg cgtcgctgtt tggcgcgttc tggggcggct
    16321 tgctgtcgtg gaccagttcg atggccggcg cggcgctgtg cttttttatc gccagagtga
    16381 tgggccgcga agtggtggaa aaattaaccg gcaaaaccgt gcttgacagt atggacggct
    16441 ttttcactcg ctacggcaaa cacaccatcc tggtctgtcg gttattgcct tttgtccctt
    16501 tcgatccaat cagctatgct gccggtctga cttcaatacg ttttcgctcg ttttttatcg
    16561 ccaccgggct tggtcaatta ccggcgacta ttgtttattc ctgggcaggc agcatgttaa
    16621 caggcggtac tttctggttt gtcaccgaac tgtttattct gtttgccctg accgtggtga
    16681 tttttatggc gaagaaaata tggcttgagc gccagaagag gaatgactga tgggtttacc
    16741 gccgcttagc aaaattcctt tcattttacg tccgcaggcg tggctgcatc gtcgccatta
    16801 cggcgaggtg ctaagcccga ttcgctggtg ggggcggatc ccgtttatct tttatttggt
    16861 gtcaatgttt gttgggtggc tggagcgcaa acgctcaccg ctcgatccgg tagtgcgatc
    16921 gcttgtcagc gcgcgcattg cgcaaatgtg cctgtgtgag ttttgcgtgg atatcaccag
    16981 tatgaaagtc gccgagcgta ccggcagcac cgataaactg ctggcagtgg ctgactggcg
    17041 gcaaagcccg ctctttagcg atgaagaacg gctggcgctg gagtacgccg aagccgcaag
    17101 cgtcacaccg ccaacagtcg atgatgccct gcgtacccga ctggctgcgc attttgacgc
    17161 tcaggcgctc accgaactga cggcattgat cggcctgcaa aacctgtcgg ctcgttttaa
    17221 ttctgccatg gacattcccg ctcaggggct gtgccgtatt ccggaaaaac gttcttaagg
    17281 agggaagatg cgccattgtg ggtggttgct gggattgtta tcgctgtttt ctttggcaac
    17341 acatgccagt gactggcagg aaattaaaaa tgaggccaaa gggcaaaccg tctggtttaa
    17401 cgcctggggc ggcgataccg caattaaccg ctatctcgac tgggttagcg gcgagatgaa
    17461 aacccattac gctataaacc tgaagattgt tcgtctcgcg gatgccgcag acgcggtgaa
    17521 gcgcattcag accgaagctg ctgccggtcg taaaacgggc ggctcggtgg atctgctctg
    17581 ggtgaacggc gaaaacttcc gcaccttaaa agagtaatgc cagtcagtta agcaactgac
    17641 tggcatt
//
LOCUS       DAZXUL010000090        17488 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZXUL010000090 DAZXUL010000000
VERSION     DAZXUL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17488
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.090"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..290
                     /locus_tag="ACH3L1_002939"
     CDS             <1..290
                     /locus_tag="ACH3L1_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFT4077598.1"
                     /translation="QQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGI
                     SESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
     gene            290..5560
                     /gene="traI"
                     /locus_tag="ACH3L1_002940"
     CDS             290..5560
                     /gene="traI"
                     /locus_tag="ACH3L1_002940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFT4077599.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRTATSEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5580..6326
                     /gene="traX"
                     /locus_tag="ACH3L1_002941"
     CDS             5580..6326
                     /gene="traX"
                     /locus_tag="ACH3L1_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFT4077600.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6381..6941
                     /locus_tag="ACH3L1_002942"
     CDS             6381..6941
                     /locus_tag="ACH3L1_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFT4077601.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            7073..7285
                     /locus_tag="ACH3L1_002943"
     CDS             7073..7285
                     /locus_tag="ACH3L1_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFT4077602.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            7531..7992
                     /locus_tag="ACH3L1_002944"
     CDS             7531..7992
                     /locus_tag="ACH3L1_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFT4077603.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8038..8247
                     /gene="hha"
                     /locus_tag="ACH3L1_002945"
     CDS             8038..8247
                     /gene="hha"
                     /locus_tag="ACH3L1_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFT4077604.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            8285..8875
                     /locus_tag="ACH3L1_002946"
     CDS             8285..8875
                     /locus_tag="ACH3L1_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFT4077605.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            9030..9503
                     /locus_tag="ACH3L1_002947"
     CDS             9030..9503
                     /locus_tag="ACH3L1_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077606.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
     gene            9750..9899
                     /locus_tag="ACH3L1_002948"
     CDS             9750..9899
                     /locus_tag="ACH3L1_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFT4077607.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            10184..10432
                     /locus_tag="ACH3L1_002949"
     CDS             10184..10432
                     /locus_tag="ACH3L1_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFT4077608.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            10677..10751
                     /gene="tap"
                     /locus_tag="ACH3L1_002950"
     CDS             10677..10751
                     /gene="tap"
                     /locus_tag="ACH3L1_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFT4077609.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10744..11601
                     /gene="repA"
                     /locus_tag="ACH3L1_002951"
     CDS             10744..11601
                     /gene="repA"
                     /locus_tag="ACH3L1_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFT4077610.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            12301..12441
                     /locus_tag="ACH3L1_002952"
     CDS             12301..12441
                     /locus_tag="ACH3L1_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077611.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLT
                     CR"
     gene            12514..12783
                     /gene="yacA"
                     /locus_tag="ACH3L1_002953"
     CDS             12514..12783
                     /gene="yacA"
                     /locus_tag="ACH3L1_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFT4077612.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQFERGDVLPHDMVEFSAAAWRDEMSRKIADK"
     gene            12783..13061
                     /gene="yacB"
                     /locus_tag="ACH3L1_002954"
     CDS             12783..13061
                     /gene="yacB"
                     /locus_tag="ACH3L1_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFT4077613.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSVSSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            13384..13962
                     /locus_tag="ACH3L1_002955"
     CDS             13384..13962
                     /locus_tag="ACH3L1_002955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077614.1"
                     /translation="MSKYSCINIIKTKAKKMVHSNTALTQVQALELIAQNSNFSSYHE
                     LIQVAKKTPLESRLLIAAFGDSDFKDVIYNLYGSCTDGPYPSLEIAVEEELSDLIATT
                     NANGFTIEDLEITDAFYDDTKGILHLEVAFLYQGDQLPDHVYSGTEFEVEAKVRLSWR
                     SEKWNFINEDFEVISVVSDTERDWFEEESEEI"
     gene            complement(14556..15665)
                     /gene="wzz(fepE)"
                     /locus_tag="ACH3L1_002956"
     CDS             complement(14556..15665)
                     /gene="wzz(fepE)"
                     /locus_tag="ACH3L1_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFT4077615.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            16540..16710
                     /locus_tag="ACH3L1_002957"
     CDS             16540..16710
                     /locus_tag="ACH3L1_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFT4077616.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
     gene            17356..>17488
                     /locus_tag="ACH3L1_002958"
     CDS             17356..>17488
                     /locus_tag="ACH3L1_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFT4077617.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
BASE COUNT         4515 a         4160 c         4893 g         3920 t
ORIGIN      
        1 cacaacagcc agtgtcttct gtcatcaatg ataagaagtc agatgcaggt gtgagtgttc
       61 cggcaggggg gatcgagcag gagctgaaaa tgaaaccgga agaagagatg gaacagcaac
      121 tgccacccgg gatcagtgaa tccggtgaag tggtggatat ggccgcttat gaggcatggc
      181 aacaggaaaa tcatccggac atccagcagc atatgcagcg tcgtgaagag gtgaacatta
      241 atgtgcaccg ggagcgcggg gaggatgttg agccgggaga tgatttctga tgttgagttt
      301 cagcgttgtt aaatcggcag gcagtgccgg aaactattac accgacaagg ataattacta
      361 tgtgctgggc agcatgggag aacgctgggc cggtcagggg gctgaacagc ttgggctgca
      421 gggcagcgtc gataaggatg tttttacccg tcttctggag ggcaggctgc cggacggggc
      481 ggatttaagc cgcatgcagg atggcagtaa taagcatcgt cccggctacg acctgacctt
      541 ctccgccccc aaaagtgtct ccatgatggc catgctcggg ggggataaac gtcttattga
      601 tgcacataac caggccgtgg attttgctgt ccgtcaggtg gaggcgctgg cttccacacg
      661 ggtgatgacg gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt
      721 taaccatgac accagtcgcg atcaggaccc acagttacac acgcatgtgg tggtggctaa
      781 tgtcacgcag cataacggcg agtggaagac actgagcagt gacaaagtgg ggaaaaccgg
      841 attttccgag aacgtgctcg cgaaccggat tgcgttcggg aaaatatatc agagtgagtt
      901 acgtcagcgc gttgaggcgc tgggttatga aaccgaagtg gtcggtaagc acggtatgtg
      961 ggagatgccg ggcgtaccgg tggaggcctt ttccagtcgt tcacaggcta tcagggaggc
     1021 cgtcggtgaa gacgcctcgc tgaaatcccg ggatgtggcg gcactggata cgcgaaaatc
     1081 caaacagcac gtcgatccgg aaatcagaat ggctgaatgg atgcagacgc tgaaggaaac
     1141 cgggttcgac atccgggcat atcgtgacgc ggcggatcag cgtgcggaga tccgcacgca
     1201 ggcgcccggg cctgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggcgat
     1261 tgccggatta agtgaacgaa aagtgcagtt cacgtatacg gacgtgctgg ccaggacggt
     1321 cggcatactg ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc
     1381 catcagccgt gagcagctta tccccctcga ccgtgagaag gggctgttca catcaggaat
     1441 tcatgtgctc gatgagctgt ccgtccgggc actcagtcgt gacatcatga aacagaaccg
     1501 ggtgaccgta catccggaga aaagtgtccc ccggacggct ggttacagcg atgcggtcag
     1561 tgttctggca caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg
     1621 gcagcgtgag cgggtggctg aactggtcat gatggcccgg gaacaggggc gggaggtgca
     1681 gattatcgct gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg
     1741 tgaactgata accggacgtc gtcagctgct ggaaggcatg gccttcacgc cgggcagtac
     1801 cgttatcgtc gaccagggtg aaaaactctc cctgaaagag acgttaaccc tgctggatgg
     1861 tgccgcacgt cataacgtac aggtcctgat aaccgacagc gggcagcgaa ccggtacagg
     1921 cagtgcgctg atggccatga aggatgccgg ggtgaacaca tatcgctggc agggcggaga
     1981 acagcgaccg gccaccatca tcagtgaacc ggaccgtaat gtccgctatg cccggctggc
     2041 cggagatttt gcggccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggggt
     2101 acgggaacag gccatactga cacaggccat tcgcagtgag ctgaaaacgc agggcgtgct
     2161 cggacaccct gaggtgacca tgactgcact gtcaccggtc tggctggaca gccggagccg
     2221 ttatctgcgg gatatgtacc gaccggggat ggtgatggag cagtggaacc cggagacacg
     2281 cagtcatgac cgctatgtta tcgaccgggt gacggcgcag agtcacagcc tgaccctgcg
     2341 ggatgcgcag ggcgaaacgc aggtggtgcg tatttcctcc ctggacagca gctggtcgct
     2401 gttccggccg gaaaaaatgc cggtggcaga cggcgagcga ctgagggtga cagggaaaat
     2461 tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc
     2521 gatgacggtt gttgtgccgg ggcgggctga accggccacc ctgcctgtgg ctgattcacc
     2581 gttcacggca ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagtga
     2641 cagtgcgacg gtgtttgcct ccgtcacaca gatggcaatg gacaacgcca ccctgaacgg
     2701 tctggcccgc agcggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc
     2761 ggaaaaactc gcccgccatc cgtcctttac ggtggtttct gagcagataa aggcgcgtgc
     2821 cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc
     2881 acagcaggcc attcatctgg cccttcctgt gctggaaagt aaaaacctgg ccttcagcat
     2941 ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa ggaaccggtt ttactgaact
     3001 gggaggggaa atcaatgcgc agataaaacg cggtgactta ctgtatgtgg atgtggcaaa
     3061 aggctatggc acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct
     3121 tcgccatatt ctcgaaggca aggaggcggt cacgccgctg atggagagag tacccggcga
     3181 actcatggag acgttaacgt cgggacagcg tgccgccacc cgtatgatac tggaaacgtc
     3241 cgaccgtttc acggtggtac aggggtatgc cggtgtgggt aagaccactc agttccgggc
     3301 ggtgatgtca gccgtgaaca tgctgccgga gagtgagcgt ccccgggttg tggggctggg
     3361 gcccacgcac cgtgcggtcg gtgagatgcg cagcgccggc gtggatgcac agacactggc
     3421 gtcctttctg catgacacgc agctgcagca gcgcagcgga gaaacgccgg atttcagcaa
     3481 cacgctgttt ctgctcgatg agagctcaat ggtgggcaat accgacatgg cacgggcata
     3541 cgccctgatt gcggccggtg gcggtcgtgc tgtggccagc ggtgacacgg accagctgca
     3601 ggccatcgcg cccggtcagc ctttccgtct ccagcagacg cgcagtgctg ccgatgtggc
     3661 catcatgaag gagattgtgc gtcagacgcc ggaactgcgg gaggcggtat acagcctgat
     3721 taaccgggat gtggaaaggg cactgtccgg gcttgagagt gtgaaaccgt ctcaggtgcc
     3781 acgtctggag ggcgcatggg caccggagca ctccgtgacg gagttcagtc acagccagga
     3841 agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa ggcgaggctt tcccggatat
     3901 ccccatgaca ctgtatgagg ccattgtccg cgactatacc ggcaggacac cggaagcacg
     3961 ggagcagacg ctgattgtca cgcacctgaa tgaggaccgg cgcgtactga acagcatgat
     4021 tcatgatgca cgggaaaagg ccggtgagct gggcaaagag caggtcatgg tgcctgtcct
     4081 gaacacagcg aatatacgtg acggagaact gcgtcgtctc tccacctggg agaaaaaccc
     4141 ggatgccctt gccctggtgg atagtgtgta tcaccggatt gccggtatca gcaaggatga
     4201 cgggctgata accctgcagg atgcggaagg taacacgcgg ctgatttcgc cccgggaggc
     4261 ggtggctgaa ggtgtcacac tgtacacccc ggacaaaatc cgggtgggga ccggcgaccg
     4321 gatgcgcttc acgaagagtg accgggagcg cggttatgtg gccaacagcg tctggacggt
     4381 gacagcggtt tccggtgaca gtgtcacgct gtcggacgga cagcagaccc gggtgattcg
     4441 ccccggtcag gagcgggcag agcaacatat tgacctggcc tatgccatca ctgcccacgg
     4501 tgcgcagggg gcgagtgaaa cctttgccat cgcgcttgaa ggcacggaag gtaaccggaa
     4561 acagatggcc ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca
     4621 ggtgtacacc gataaccgtc agggctggac ggatgccatt aacaatgccg tacagaaagg
     4681 aactgcccac gatgtgctgg agccgaaacc ggaccgggag gtcatgaatg cgcagcggct
     4741 gttcagtacg gcacgggaac tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc
     4801 ggggctggcc gggggagaca gtccggcacg gtttattgca ccggggcgca aatatccgca
     4861 gccgtatgtg gcactgcctg cttttgaccg taacggcaag tccgccggta tctggctgaa
     4921 cccgctgacc acggatgacg gaaacgggct gcggggattc agcggtgaag gacgggtgaa
     4981 aggcagcggg gatgcgcagt ttgtggccct gcagggcagc cgtaacggcg agagcctgct
     5041 ggctgataat atgcaggatg gtgtccggat tgcccgtgat aaccccgaca gcggtgtggt
     5101 ggtgagaatc gccggtgaag gtcgtccgtg gaatcccggt gccatcaccg gtggtcgcgt
     5161 gtggggggat atcccggaca acagcgttca gccgggagcc ggaaatggcg aaccggtcac
     5221 ggcagaggta ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg
     5281 cgcagatgaa attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac
     5341 agagcaggct gtcagggaga ttgccggaca ggagcgtgac cggactgcca cttctgaacg
     5401 ggaagccgcg ctgccggaga gtgtgctgcg tgaatcacaa cgggagcggg aagcggtccg
     5461 ggaggttgcc cgggaaaatc tgctgcagga gcgtctgcag cagatggagc gggatatggt
     5521 tcgtgacctg cagaaagaga aaaccctggg cggagactga tacaggaaga taaacgctga
     5581 tgacaacgga taacacgaac acgacacgta acgattcact ggctgcacgg accgatacct
     5641 ggttgcagtc atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac
     5701 tggtgctgat ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt
     5761 ttctggccgg gcgaggagcc tttccgctgt ttgccctggt gtgggggctg aatctgtccc
     5821 gtcatgcgca tatccggcaa ccggccatta accggctgtg gggatggggg attattgccc
     5881 agttcgcata ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg
     5941 cagtggcggc acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg
     6001 ccattcttct gatggccctg tgggggcctt tgtccggcac cagttacggc attgccgggc
     6061 tgctgatgct ggcagtcagc caccggctgt accgggcgga agacagagcg gaacgtctgg
     6121 cgctggtcgc ctgtctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag
     6181 cggcggtggc tggtcttgtg atgacggtgc tgaccgttgg tctggtgtcg tgtgcaggaa
     6241 aatcattacc ccgtttctgg cccggggatt ttttcccggt attctacgcc tgtcatctgg
     6301 ctgtgctggg cgttctggcg ctgtgacggg tgtggtatct ttgaccgtaa caggatgatt
     6361 cgtcagaggc agaacacagc atgacagagc agaagcgacc ggtactgaca ctgaagcgaa
     6421 aaacggaagg ggagacgcct gtccgcaggc ggaaaaccat catcaatgtc accacgccac
     6481 caaagtggaa ggtgaaaaag cagaagctgg ccgagaaagc agcccgggaa gcggagctgg
     6541 cggcaaaaaa agcgcaggcc agacaggcgc tgtccattta tctgaacctg ccctcgctgg
     6601 atgaggccgt gaacaccctg aaaccctggt ggccgggatt atttgacggt gaaacgcccc
     6661 ggcttctggc ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag cggaatatcc
     6721 cgctctcgca taaaaaactg cgcagggcgc tgaaggccat cacccgttca gaaagctatc
     6781 tgtgtgccat gaaagccggt gcctgccggt atgatacgga agggtatgtg acggagcata
     6841 tttctcagga ggaggaagcg tatgcggcag cgcgtctgga taaaatccgc cgccagaaca
     6901 ggataaaggc agagcttcag gccgtgcttg atgagaaata aaaaaaagcc tccatcagag
     6961 aggaggcagg gaaataaggc ttaaaaggaa ataacttccg tacccgaaag gaagccaatt
     7021 cagtagatta tttaacacca taatactgac gggttaaaga ggagtgataa agatgaatgg
     7081 attcagaaac agttcacgga acggtcaggt ctggcgctgg cagcgtgccg gaagccgggc
     7141 cgttattctg gaggtcagtg gacgctggat ggaagcggca gaagcatggc gacgggctgc
     7201 cggtgttgcg ccccggacag actggcagca gtttgcccga aaaagggctg aacactgcca
     7261 ccggcgcagc cggggaaggg tgtaacgaaa aaagcagcct tcattactcc agggattcag
     7321 aaggctgaat aatgcatgag atttgctttt tttaatatcc ggtcgggcag tatgaccgga
     7381 cagttctgag tagcacagag ttttttccgt attaagtcgt gattttatat tttgtgatta
     7441 atttcacaaa ataaggtgtt attcagtgtg tgctgcgata ttcaggatta cctgaaatac
     7501 aggtgccatt tatctgatat ggagaataac atgaggaaat atattccact ggtattattt
     7561 atcttttcat ggccggtatt gtgtgcagat attcatggac gggttgttcg tgtactggac
     7621 ggtgatacca tcgaggtcat ggactcacgg aaggcagtgc ggatcaggct ggtaaatatt
     7681 gatgcaccgg aaaagaaaca ggattatggc cggtggtcaa cggacatgat gaaatcactg
     7741 gttgcaggaa aaacggttac cgtcacatat tttcagcgag atcgttatgg tcgtattctc
     7801 gggcaggttt atgcaccaga cggaatgaat gttaatcagt ttatggtccg ggccggggct
     7861 gcctgggtat atgagcagta caacacagat cccgtgctgc ctgtccttca gaacgaagcc
     7921 agacaacaaa aacgtgggct ctggtcagat gctgatccgg ttcccccctg gatatggaga
     7981 caccgtaaat aaagcgatgg caatcttatc attcatctgt tctcagcggg aataaaaatg
     8041 gcgaaaacaa aacaggaatg gctgtatcag ttacggcgat gttcatcagt caatacgctg
     8101 gaaaaaatta ttcataaaaa cagagacagt ttattaaaca gtgaacgtga atcatttaat
     8161 tcagcagcag accaccggtt agcggaatta atcaccggaa agttatatga caggatacca
     8221 aaagagatat ggaaatatgt aaggtaataa ttcagaataa ttaattacca ggagcagata
     8281 aacaatgaag ctgattattt ttattctcat tgttctgatt attgcagcgt tacttatccg
     8341 gattatcctc aaaagtgtaa atcagcattc tcctttgctg atgcagttgc atgcggcagg
     8401 tatacggacc ggtgatgcag agcgaatact gtccggcggt gaatactggc aacgtcagaa
     8461 gacgctgctg acagaaaggg aagtcagttt tatgaaagga ctgttcagaa ttgtggatat
     8521 gaagcggtgg tatctgtgtc cgcaggtacg ggtcgcggat atcgtccagc tgaacgggaa
     8581 tatccggcca cgatcacgcc agtggtggca gttattcagg atggtgtctc agtggcatgt
     8641 tgatgtggtt atcgttgaac ggcgttcgtt cagtattgtt gcagcagtag agctggatga
     8701 tgccagccat ttacgaccgg aacgccggcg ccgggatatt cttctggaag aggttctgag
     8761 gcaggctggt attccgttgc tcagaagcca cgatgccaga aaactgctgc agatgaccgg
     8821 agaatggctg aatacaacag gggctgatca gcagtccccg gaacatcgta gctgacgcct
     8881 tcgcgttgct cagttgtcca accccggaaa cgggaaaaag caagttttcc ccgctcccgg
     8941 cgtttctgta ataaaaagcg ggaaatggtg gtcacagagt aaattccgga accacctgta
     9001 cccaaacttt atataataat ggtgaatata tgaaatatga tgatgtatca gaattgatga
     9061 ttaagcgtga acacaatatt cttattgttg ggaactctgc ctcccacatt gataagtttt
     9121 ttaaggggta ttctatacca ggttctaaat attatgattt cactcagatt aacagtgatt
     9181 cagatgtaga gagaaatgag gatgctgtct cctttatcag ggatgcaatg aatagtgaag
     9241 agcagacaat catttttaat tgtgctgggt ggcctgattt aggtggtgga tcagcagtat
     9301 cacagtttgc aatgatggtc agaaaatttg gtaaacaact tattgtcgct gtaagtgaaa
     9361 aggatgccat caaattaaaa gacaactttg atattattgg gatgttgtca tatggtaaag
     9421 aaaattttat tgccatgagt catacgaaaa acgagttaac tggaggaaaa cgtcggtacc
     9481 ggattaaaaa tggatgtaaa taaaaagcat acttattggc tggagtgacg ccttcgtgct
     9541 tctgtagtaa aaagcgggca atgcaggtaa tagtcagcaa cagggaatgt ggtattatcg
     9601 cggcgggtgt ctgagccttt ctggttcagg caagacgcag gtaccagaaa tgcaaagacc
     9661 ccacttgtta atccattaac tcgtgaggtc tgcatgaagt acattgcact actgattgta
     9721 gcctcttcct tgcagagagg tcaaagttta tgacgaagta tgcccttatc gggttgctcg
     9781 ccgtgtgcgc cacggtgttg tgtttttcac tgatattcag ggaacggtta tgtgagctga
     9841 atattcacag gggaaataca gtggtgcagg taactctggc ctacgaagca cggaagtaaa
     9901 ctgccgggcg gggacggaag tccccgcttt ccggaagtgt gaggtatttc aggggcagac
     9961 acccgacatg ccagaaacag ccggtcccgc ccggggccgg ctcccgggtt aaggcatttc
    10021 ctgttttttc agccatttcg caacaaaaat cacattaaac aacagtaatc cccgttgatt
    10081 tgtgcgccaa cacagatctt cgtcacaatt ctcaagtcgc tgatttcaaa aaactgtagt
    10141 atcctctgcg aaacgatccc tgtttgagta ttgaggaggc gagatgtcgc agacagaaaa
    10201 tgcagtgact tcctcatcag gtacaaagcg agcatacaga aaaggtaatc ctctgacgct
    10261 tgcagaacgt cagcaggcat ccctggcaag aaaacgagct acacataaag aactcagagt
    10321 gttcattcct gctgcgttaa aagcgcaatt gcaggtgatg tgtgaagctg agggcgttac
    10381 ccaggctgag atgattgccg aactgataaa gcagaagagt gcttttagct aactaaaatg
    10441 tagcgacatc acattcttgc ctgtttctgg tgtgcgtgat agattgctga tcgtttaagg
    10501 aattttgtgg ctggccacgc cgtaaggtgg cagggaactg gttctgatgt ggatttacag
    10561 gagccagaaa agtgaaaacc ccgataatct tcgacaagtt tggcgactga gaagattacc
    10621 ggggccatct aaaaaccgta tagccaacaa ttcagctatg cggggagtat agttatatgc
    10681 ccggaaaagt tcaagacttc tttctgtgct cactccttct gcgcattgta agtgcaggat
    10741 ggtgtgactg atcttcaaca aacgtattac cgccaggtaa agaacccgaa tccggtgttt
    10801 acaccccgta aaggtgccgg aacgctgaag ttctgcgaaa aactgatgga aaaggcggtg
    10861 ggcttcactt cccgttttga tttcgccatt catgtggcgc atgcccgttc gcgtggtctg
    10921 cgtcgacgca tgccaccggt gctgcgtcga cgggctattg atgcgctcct gcaggggctg
    10981 tgtttccact atgacccgct ggccaaccgc gtccagtgct ccatcaccac gctggccatt
    11041 gagtgcggac tggcgacgga gtctgctgcc ggaaaactct ccatcacccg tgccacccgg
    11101 gccctgacgt tcctgtcaga gctgggactg attacctacc agacggaata tgacccgctt
    11161 atcgggtgct acattccgac cgatatcacg ttcacatctg cactgtttgc tgccctcgat
    11221 gtatcagagg aggcagcggc cgccgcgcgc cgcagccgtg tggtatggga aaacaaacaa
    11281 cgcaaaaagc aggggctgga taccctgggc atggatgaac tgatagcgaa agcctggcgt
    11341 tttgttcgtg agcgttttcg cagttatcag acagagctta agtcccgggg aataaagcgt
    11401 gcccgtgcgc gtcgtgatgc ggacagggaa cgtcaggata ttgtcaccct ggtgaaacgg
    11461 cagctgacgc gcgaaatcgc ggaagggcgc ttcactgcca atcgtgaggc ggtaaaacgc
    11521 gaagttgagc gtcgtgtgaa ggagcgcatg attctgtcac gtaaccgtaa ttacagccgg
    11581 ctggccacag cttccccctg aaagtgacct cctctgaata atccggcccg cgccggaggc
    11641 attcgcacgc ctgaagcccg tcgatgcaca aaaaaacagc accacataca aaaaacaacc
    11701 tcatcatcca gcttctggtg catccggccc cccctgtttt cgatacaaaa cacgcctcac
    11761 agacggggaa ttttgcttat ccacattaaa ctgcaaggga cttccccata aggttacaac
    11821 cgttcatgtc ataaagcgcc agccgccagt cttacagggt gcaatgtatc ttttaaacac
    11881 ctgtttatat ctcctttaaa ctacttaatt acattcattt aaaaagaaaa cctattcact
    11941 gcctgtcctg tggacagaca gatatgcacc tcccaccgca agcggcgggc cccgaccgga
    12001 gccattttag ttacaacact caaatacaag caccgggaaa accccagtcc agcgcagaac
    12061 tgaaaccaca aagcccctcc tcataactga aaagcggccc cgccccggcc cgaagggccg
    12121 gaacagagtc gcttttaatt atgaatgttg taactacatc atcatcgctg tcagtcttct
    12181 ggctggaagt tctcagtaca cgctcgtaag cggccctcac ggcccgctaa cgcggagata
    12241 cgccccgact tcgggtaaac cctcgtcggg accactccga ccgcgcacag aagctctctc
    12301 atggctgaaa gcgggtatgg tctggcaggg ctggggatgg gtaaggtgaa atctatcaat
    12361 cagtaccggc ttacgccggg cttcggcggt tttactccgg tatcacatgt aaccacagcg
    12421 tgccgcctta catgccgctg acgcggcata ttctggtgac gatatctgag tcgttatata
    12481 ctgtgtatat acgtagtaat gacgaggtga taaatggcac aggttaatat gagtttaaga
    12541 atcgacgctg aactgaagga tgcttttatg gccgctgcaa aaagcatgga ccgtaatggc
    12601 tctcagttaa tccgggattt tatgcgccag accgttgaac ggcagcataa tacctggttc
    12661 cgtgaccagg ttgctgcagg acgtcagcaa ttcgagcgcg gcgatgtgct tccccatgac
    12721 atggtcgaat tttccgctgc tgcatggcgg gatgagatga gcaggaagat tgccgataaa
    12781 tgatggagat attctggacc atgctggcca gccaggacag gaagcgcatt cgtgagtaca
    12841 ttgcggagca gaacctgatg gccgccattg aactggacga acgaattggt tattcagtca
    12901 gcagcctggc cgggcagccg tataaaggcc gcaacggtcg tgtggaaggt acccgggaac
    12961 tggttattca cccgcatttt gttctggttt atgaggtcga cagccagtgg ggaaaagtgt
    13021 atatcctgcg cgtattgcat actgcacaga agtggccata gaggctgtat ggaatcagtt
    13081 gtgaatcatg acgaagaaac attggggcga gcactgatag ggggcagtct ggagaacgga
    13141 aattattgta cggtaagcca gttttctaac caggctgagt gttgtttctc aatttgactt
    13201 aatcagctga tcttgccact ataacgctag gtggtgatgg tcacctggcg cctgtctcgc
    13261 aaagcaatag cccatcttta ttaggtttta cctacatcat acttggctcg ccacatttgt
    13321 gacgctcaga gtccggttag caggctatcg gttctaaaag cgtctgaaat ttggagaaca
    13381 gccatgtcaa aatattcttg tattaacatt atcaaaacca aagcaaaaaa aatggtgcac
    13441 tctaatactg cactcacgca agtccaagcg ttagagctta ttgctcaaaa ttcgaatttt
    13501 tcgagttacc atgaacttat ccaggttgca aaaaaaacac cgttggaatc tcgcttgttg
    13561 atagctgctt ttggtgattc tgatttcaaa gatgtcattt ataacttata cggcagttgt
    13621 accgacggtc cctacccttc tcttgaaata gctgtagagg aagagttatc agacttgatc
    13681 gcaactacca atgcaaatgg ttttaccatt gaagacctag agattacaga tgcattttac
    13741 gatgatacaa agggtatttt gcatttggaa gttgcattcc tttaccaggg cgaccagcta
    13801 ccagaccatg tttactccgg tacggaattc gaagttgagg ccaaagttcg gttgtcatgg
    13861 cgcagtgaaa aatggaattt tatcaatgag gattttgaag tcattagcgt agtatccgat
    13921 actgagcgtg attggttcga ggaagaatca gaagagatct gacagtagga cggtaagcct
    13981 gaattgatac aggcttaccc atagcaatag gggacactct gatgcgagat ataaagagtg
    14041 atctcatagg gggcagtttg gatatagaga attattgtac ggtaagtctg ttttttgaac
    14101 agatttacct atcgcgatag ggtgtactcc cgcgctatac gctgttttca ggggatttga
    14161 actcaatcgg ataacctgaa tgcaataccg caatggcgat aatttaaaag cgatagccgt
    14221 tgacgcaaaa tcaatccgtt acgcatctct attggttaaa taatgcgctt aacgtacaaa
    14281 attttcccat atccaaactg acccctattg agagttcttg aagtggcagc cttactgagg
    14341 ctacactaga aggacagatt tggtgacatg tctcgcacaa gactgttacc acggttcaga
    14401 agttccccaa cttactgaac cttcgaaaaa ccacctcccc aggtggtttt ttcgttttct
    14461 agagtagaag actacggctg taagggaatg atctcaagaa gatcctttga actttttata
    14521 aaagttgctt ataaaatgat gcatcagcac aacttttact caatcacact catctttctg
    14581 gcctccatgg catgacgtaa aagtatatag ccacatgcaa gcatccctcc aatgacagaa
    14641 gaaagaatta caattatggc attacttggc ccatcctttc tgaccggtaa cgttgggctc
    14701 aattgatact gaaatggcat aaaatcaaca tcccccactt tcgccacaac caattgattc
    14761 acaacatatt gacgatttcg caactcccca ctcaattccg aaacatcctt gattgatttt
    14821 ttgatgttca atttagttgc tataccatca gaaccgagag ccacaggaaa atctgggtca
    14881 tccttcataa tctgaccatt gctgtataca ggttttttta ttccagccgc attagcaacc
    14941 tccagtgaat aattgaggcg tcgaatatct gcattcagct ggtttctaat ttttatctca
    15001 tccagtgcaa gaatttcttt ttcaacatta gttttaactt ctagtttatt tcttatatct
    15061 tccatcaaat cccttacaac aagggaagaa acataattaa tatacccaga caacacatcg
    15121 tgagcttctt tacttgttgg agcagtaaaa ctcagagtcc atgatacata caatgatgac
    15181 ttgtcgtttt tcttactggc attatcatca atcgacttca tatttttact caactcaaca
    15241 attgcacgat gcagttcgag agcgctaatg ttattctttt taataatctc cataacataa
    15301 ggagatgatc tcatatactc attcaacgaa ttaacggact gaaatttctt aataaacaga
    15361 gcaaatgcgc tatcacgatt gatatcaaaa tccactccca gaacatgaag cttggaaagc
    15421 tccttattta attcactcca ttgaacagac tcagcaggag taaccacagc agaactagtc
    15481 catttttgtg gcaaaacaaa agaaacaaga aagccaatac aagcaaaaaa tagaatcacc
    15541 ccaacaattt ttatttttgc tgcccataaa acgctaatca agccaaaaag gtcaatttct
    15601 tcattaccat tcggcaacat gttgtaatta gaaaaatgtt cgtcagagac ctttgagatt
    15661 ttcattgaaa aaaacacaat atattaacat atggtttttt tattctatca tatgttagga
    15721 aaaaacctaa aaaccattca gcagacattt aacataacat tttattgcgc gcaccgctaa
    15781 aacgcgctga acgcgattct gccgcaaaca ccgcaaaaag agatgttttc cccggggtaa
    15841 accggaaaaa tgcgtcagga tcgttttcag ctcactgcat agctatgcat gaaagtgaat
    15901 gacgatcggt ttgggcgcat acagtgccca tgagacacat tttcggacat tttcagttcg
    15961 tgaatccctg actcattgct acatatctga acagacaccc ctagcagcgc ccccaggggc
    16021 gtcctgtaaa aaaacacacc gcgccgctag cagcacccct aatataagat aatgtttttt
    16081 ttatgaaaat agtcagtacc acccctaaaa agcggtgttg gcgcagctac aacaccgcgc
    16141 cgacaccgct tttttaaata tcacaaagag agcaagaaga actaatttgt cataactctc
    16201 tatttataaa gaaaaatcag caaaaacttg tttttgcgtg gggtgtgggc atttcaggca
    16261 tgagaacaga ctcgcgtttt tccggagtat cagcggaatt attgattaag aaacactcga
    16321 cgcagagcga aattttatgc aacagcggct gcactgcaca gggattattt atcagttgat
    16381 atgctgtgcc atgatacgtc ctaaagaaac aggacaggat gtgcaattgt aataccgtcg
    16441 cccgcaacgt cttacaatta ccacctgctc agggtccccc ccccgacacc cggaactgac
    16501 ggagaacaac ggaaggtgaa caaataacaa caaactgcgc tgaatatggc gcgatgtatc
    16561 aaaagccaga gcctgacact gcttgaaaaa ctggatgctc tggatgccga cgaacaggcg
    16621 gccatgtgca aacgactgca cgaactcgca gaagaactcc agaacagcat ccagattcgc
    16681 tttgaggccg aaagtgaaac aggaacataa cgaagctgac ggaggcggtc acggctttcc
    16741 cgtgggcgtt ttcgtcagca gacaagcccg tcagggcgcg tcagggggtt ttagcgggtg
    16801 tcggggcgca gccatgaccc agtcacgtag cgataacgga gtgtatactg gcttaattat
    16861 gaggcggcag agcagatggt gagagaacag cgacatatga ggtgtgaaat gctgcacaga
    16921 tacgtaagga gaaaatgcag cttccggcgc tcttccgctt cctcgctcac tgactcgctg
    16981 cgctcggtcg ttcggctgcg gcgagcggtg tctgctcacg caaagacgga tatacggtta
    17041 tccacagaat caggggataa caccagaaaa aacatgtgag caaaaaagag gccccgaaaa
    17101 aaggccgcgc cggtggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    17161 cgacgctcaa gtcagaggtg gcgaaacccg acaggactta aagataccag gcgtttcccc
    17221 ctggtggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
    17281 tgttatggcc gcgttcgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
    17341 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    17401 aactatcgtc ttgagtccaa cccggtaaga cacgacttaa cgccattggc agcggccact
    17461 ggtaaacagg atgtgcgaga gatgtatt
//
LOCUS       DAZXUL010000091        17451 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZXUL010000091 DAZXUL010000000
VERSION     DAZXUL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17451
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.091"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..167
                     /locus_tag="ACH3L1_002959"
     CDS             <1..167
                     /locus_tag="ACH3L1_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFT4077618.1"
                     /translation="LHVISKRYTQRIERHNLNLRQHLARLGRMSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
     gene            511..633
                     /locus_tag="ACH3L1_002960"
     CDS             511..633
                     /locus_tag="ACH3L1_002960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077619.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     gene            630..1196
                     /gene="mntP"
                     /locus_tag="ACH3L1_002961"
     CDS             630..1196
                     /gene="mntP"
                     /locus_tag="ACH3L1_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFT4077620.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(1193..2002)
                     /gene="rlmA"
                     /locus_tag="ACH3L1_002962"
     CDS             complement(1193..2002)
                     /gene="rlmA"
                     /locus_tag="ACH3L1_002962"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFT4077621.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERPDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(2168..2377)
                     /gene="cspE"
                     /locus_tag="ACH3L1_002963"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(2168..2377)
                     /gene="cspE"
                     /locus_tag="ACH3L1_002963"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFT4077622.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2390..2533)
                     /locus_tag="ACH3L1_002964"
     CDS             complement(2390..2533)
                     /locus_tag="ACH3L1_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFT4077623.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(3204..3491)
                     /locus_tag="ACH3L1_002965"
     CDS             complement(3204..3491)
                     /locus_tag="ACH3L1_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFT4077624.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3566..3709)
                     /gene="mgrB"
                     /locus_tag="ACH3L1_002966"
     CDS             complement(3566..3709)
                     /gene="mgrB"
                     /locus_tag="ACH3L1_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFT4077625.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3868..4107
                     /locus_tag="ACH3L1_002967"
     CDS             3868..4107
                     /locus_tag="ACH3L1_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFT4077626.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(4250..5041)
                     /gene="kdgR"
                     /locus_tag="ACH3L1_002968"
     CDS             complement(4250..5041)
                     /gene="kdgR"
                     /locus_tag="ACH3L1_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFT4077627.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            5218..6591
                     /locus_tag="ACH3L1_002969"
     CDS             5218..6591
                     /locus_tag="ACH3L1_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4077628.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6637..7518)
                     /gene="htpX"
                     /locus_tag="ACH3L1_002970"
     CDS             complement(6637..7518)
                     /gene="htpX"
                     /locus_tag="ACH3L1_002970"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFT4077629.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7710..9758)
                     /gene="prc"
                     /locus_tag="ACH3L1_002971"
     CDS             complement(7710..9758)
                     /gene="prc"
                     /locus_tag="ACH3L1_002971"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFT4077630.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9778..10476)
                     /gene="proQ"
                     /locus_tag="ACH3L1_002972"
     CDS             complement(9778..10476)
                     /gene="proQ"
                     /locus_tag="ACH3L1_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFT4077631.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10573..11070)
                     /gene="msrC"
                     /locus_tag="ACH3L1_002973"
     CDS             complement(10573..11070)
                     /gene="msrC"
                     /locus_tag="ACH3L1_002973"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFT4077632.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            11200..12483
                     /gene="yebS"
                     /locus_tag="ACH3L1_002974"
     CDS             11200..12483
                     /gene="yebS"
                     /locus_tag="ACH3L1_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFT4077633.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12452..15072
                     /gene="yebT"
                     /locus_tag="ACH3L1_002975"
                     /pseudo
     CDS             12452..15072
                     /gene="yebT"
                     /locus_tag="ACH3L1_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            15152..16591
                     /gene="rsmF"
                     /locus_tag="ACH3L1_002976"
                     /pseudo
     CDS             15152..16591
                     /gene="rsmF"
                     /locus_tag="ACH3L1_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16709..16945
                     /locus_tag="ACH3L1_002977"
     CDS             16709..16945
                     /locus_tag="ACH3L1_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFT4077634.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            17050..17241
                     /locus_tag="ACH3L1_002978"
     CDS             17050..17241
                     /locus_tag="ACH3L1_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFT4077635.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(17266..>17451)
                     /locus_tag="ACH3L1_002979"
     CDS             complement(17266..>17451)
                     /locus_tag="ACH3L1_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFT4077636.1"
                     /translation="YEWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHR
                     VDIGNLHYIDTGAVFGG"
BASE COUNT         4241 a         4397 c         4206 g         4607 t
ORIGIN      
        1 agctgcacgt tatcagcaag cgttacactc agcgcattga gcgacataat ctgaatctga
       61 gacaacatct ggcaaggctg ggacggatgt cactgtcgtt ctcaaaatcg gtggagctgc
      121 atgacaaggt catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta
      181 ccctcgtatc aactcaataa cttatgaatt tagccaaagc tatgtttagt gtatttttaa
      241 taatcagaca tagcttaggc tatattatct cttcccttat ttgttattta ttttaacgtt
      301 tcattgatat ataaacataa atgaaaatcg ttatcaataa agcaatgaaa taatatattc
      361 caacagttgt tttatattct caaaatatgt taaggttgcg ccctcatttg gggagtagcc
      421 gatttccaga ttccggaaat gtacgtgtca acatactcgt tgcaaaacgt ggcacgtacg
      481 gactgaagac tttcagtcag gcgagaccat atgcacatca atcgctatgc ctgcatgaac
      541 gcgaaaaccc atcgcggctt gttgttcatg tatgcatgtt tgctgggggc gatgatgtgt
      601 tttatggata ccccggtcag gacgttgtca tgaatatcac tgctactgtt cttcttgcgt
      661 ttggtatgtc gatggatgca tttgctgcat caatcggtaa aggtgccccc ctccataaac
      721 cgaaattttc tgaagcattg cgaaccggcc ttatttttgg tgccgtcgaa accctgacgc
      781 cgctgatcgg ctggggaatg ggcatgttag ccagccggtt tgtccttgaa tggaatcact
      841 ggattgcgtt tgtgctgctg atattcctcg gcgggcgaat gattattgag ggttttcgtg
      901 gcgcagatga tgaagatgaa gagccgcgcc gtcggcacgg tttctggcta ctggtaacca
      961 ccgcgattgc caccagcctg gatgccatgg ctgtgggtgt tggtcttgct ttccttcagg
     1021 tcaacattat cgcgaccgca ttggccattg gttgtgcaac cttgattatg tcgacattag
     1081 ggatgatggt tggtcgcttt atcggctcaa ttattgggaa aaaagcggaa attctcggcg
     1141 ggctggtgct gatcggcatc ggcgtccaga tcctctggac gcacttccac ggttaataag
     1201 aacgctgcca gaggtgaata ttaaagtccg tctggcagtc gaacacttct tttgctgcca
     1261 gtgtttgcca gacttctggc ttcgcacgcc aggcaaacgg cgtcatctgc aataatgcga
     1321 cggcttcatc accgcgaaga cgcatcggat aacacaactc cgcactctgc tgtaatgtaa
     1381 aaccttccag ttgttctgca tgaggtgcat gaagatgtac ttcattgtaa atcagcccct
     1441 tcagctccat caaatgtcgc ggtcccggcg tggcagtaat gacccagccg ccgggcttca
     1501 ctactcgtgc taattcttct gctttacacg gcgcgtaaat acgtattatg gcgtccatac
     1561 tggtatcgga aaacggcaaa cggtggctgg aagcgacaca aagagtgacc tgcggatagc
     1621 gtttcgccgc cgcttttatc gctaccttcg agacatccag gccaaacgtg gtgatttcgg
     1681 gcaacgcatc ggcaaatgcg tgtgtgtaat acccttcacc acagccaata tccagcaccg
     1741 ccgtggcctt ttcatcaggc cgttcccgca gttgggcgac aattgcatca cgcagcggct
     1801 gataatgtcc ggcatctaag aatgcgcggc gtgcttgcat catttccgcg ctgtcgcccg
     1861 gatcacgaga ccgtttatgc tgaacgggca gcagattgac atacccttct ttcgccatat
     1921 caaactgatg tcgttgggga cagatatagc tgtttttttc acgcgaaaga ggctgatggc
     1981 aaagtggaca ggaaaacgac atgacaactc cggcaggata ctaaaggccg caagtgtaac
     2041 gcgaatcacg cggcgggcaa atatccgaac gccaggagcg aagataaagc atagtaaaaa
     2101 gcctcgcatt cgacgaggct ttatagcgag attgaagcgt attcacactt cagatcagtg
     2161 gattcgatca gatagctgtt acgttaacag ctgccggacc tttctggccg tcctgaattt
     2221 cgaactcaac gttctgacct tcagccagag ttttgaagcc attaccctgg atagcggaga
     2281 agtgtacgaa cacatctttg ctgccatcag ccggagtaat gaagccaaaa cctttagact
     2341 cgttgaacca cttaacctga cctttaatct ttgccatttg aaaaattcct tagattgttt
     2401 tcttcgcccg caggcataac atagataaaa ctgacacatt actgcatgag gcaccaatat
     2461 aaggctcggc agagaagcgg tattcaacgt caacgtgttt actcaggact tctttactga
     2521 aaatgccaca cataaacaga actgtacctc gtttaacccg aaatctttta tcacatacaa
     2581 cgttaattat ggcaagccat ttttaaacat atctcgatca gacgcacaaa tctcgatacg
     2641 tcttccactt tttttgcaca cttatgcaac ggaatacgcg ccgatatatc attgtgctta
     2701 accttgccag ttcaggcaga tacttaaact ggcgtagttt ctaacatagt tcaataagtt
     2761 cagttgttcc aggccagagg gagaagttat tacatagtgc gtgcaatatc acattttctg
     2821 ctatgcaatg aataaaaagt tatatcactt tttctcataa aacagtcagt taacggctat
     2881 caattaccct aaagagaagt caatccccaa agggattgta aatttaaaat aagaaaaatt
     2941 gatgagtgag caaaaaaaat caagagagaa acgtttcgct aataaataat accacgctta
     3001 atcacaaatc tgatgtaaac atcctaataa attaatgagg aaaatatctt tttctgatat
     3061 cccgttacgc aaataaataa tatgataatt atttgaagtc agaccagagt tttaatttga
     3121 agcagttgca ccatgacagt gcgtataatc aggatgaatt gagtaaccct tcaacgacgg
     3181 ggttactcaa gattgaggaa ggattatcgt tcggcgacca ggcgaataat atcttcgtca
     3241 tcaaccgatt tttgactctc aatggtcttt ttatctgctt tctctggttc gtttgcttcg
     3301 cacaaacgac gtaacagtgc attttgacgt ttttgctgat ccagcaacgc ctcaagcagt
     3361 tcaatctgtt cgttagtccg tgaactggca cgattgataa aaaaccacaa gatgagcccg
     3421 ataagaagaa ccaccaccga tacaaccaaa gacgcaatgt tcatcacgcc tgaatttaca
     3481 acttcgttca tttcaccacc tctgagtaga ggcgctattc taccactgct ggagaggaag
     3541 aaaatctagt gctgaaaaaa tgatatcacc acgggataaa ctggttaatg gcacaaattc
     3601 cgctgaaaaa ttgtacatcc tgatcgcaca tcatgttgaa tacctgcgcc caaagcagca
     3661 agcaagccaa caccacgaca accagaacga cccatcgaaa ctttttcact ccaatctccg
     3721 cttcacctac cttatgtcat taaactagca tgcgttagtt aaacagcgcc taactatgtc
     3781 gatattcgtg ctttttcgga atgagtagct tgcttcagcg catattgccg ctacgattaa
     3841 gcgaacataa aaagagaaga gattgtaatg cgattcatca ttcgcacagt tatgctgata
     3901 gcactggtat ggataggatt attactcagc ggatatggcg ttttgattgg aagcaaagag
     3961 aatgccgcag gattaggttt gcaatgtacc tatctgactg ccagaggcac cagcacggta
     4021 caatacttgc acactaaaag tggtttcttg gagataacag attgcccact gttacgtaaa
     4081 agcaatatcg tggtcgataa tggttaaggg taataaataa ccagattctc actcatgagt
     4141 aaaatattct tatagcaagc gcgtttaata taacgcacct tcaactttat ttttctcatg
     4201 ctaaataaag aacctgtctg tttgatattc cgacaggttc ttactctcat cagaacggat
     4261 agtcgtgata acccatttgg gcagaaattt tgcgcgctgc ggtatgcaac attgcgacat
     4321 attcctgtaa acgctcttca gagaaacgca acgtcgggaa ggagatgctc aaaccggcaa
     4381 tgaccacgcc aaagcgatca aataccggta ccgcaatgca ttgcagccct tcttcctgct
     4441 cttcgttatc ttctccatac ccctgctcgc gcacctggtc cagaacgggt aataacgctt
     4501 ctgtactggt gatggtccgc tcggtactgc gtttatactc cacgccctca agaatttgct
     4561 tcacttcatc gcgatcgcgc catgccagca gtaccttacc aatcgcggtg ctgtacagcg
     4621 gattacgacg cccaatccgt gaatacatgc gcaaattgta catagagtca attttgtgaa
     4681 tgtaaacaat actgtcttcg tccagtgcgc cgaggtggat agtttctttg gtcaggcggg
     4741 agatctcacg catctggata tctgcgctac gaattaaatc gacgttttgt aacgcgcgag
     4801 cgcccagttc aaacaatttc agggtcaggg aatatttctc cgactcccct tcctgcgcca
     4861 cataacctaa ggttttcatg gtctgtaaaa agcgataaac ggtgcttttt gacatcatga
     4921 cgcgctgcga cagctcggtt atccctattt cgcgctcttc acccagcgcc tgcaaaatgc
     4981 caaaaacttt tagcacggaa gatacagaat caggctgttt atccagatct gcgttagcca
     5041 tttatcacct cattgcgagt gttttataaa aatcagaact gttttttatt ataatttcgc
     5101 accagggtgg tcgcaatcca tcttttgccg gttagttaca attctgcgac atccaccgtg
     5161 aatatcagtg ctagaatcat acccctgttg attattcacc aaagatataa aattcctatg
     5221 ccaaaagttc aggccgacgg cctgccattg ccccagcgat acggtgcgat attaaccatt
     5281 gtgattggta tttcgatggc tgtccttgac ggcgcaatcg ccaacgtcgc cctgccaaca
     5341 attgccacgg accttcatgc cacaccagcc agttccatct gggtagtgaa cgcctatcaa
     5401 atcgccattg tcatctccct gctctcgttt tcgtttctgg gcgatatgtt tggctatcga
     5461 cgtatttata aatgcggtct ggtcgttttt ctgttgtctt cactgttctg cgccctttct
     5521 gattcgctgc aaatgctcac ccttgcgcgt gtcatacaag gtttcggcgg tgcagcgttg
     5581 atgagcgtta ataccgcact tatccgcctg atctatccac aacgttttct ggggagaggg
     5641 atgggcataa actcgtttat tgttgccgtc tcttctgctg ccgggccgac aattgctgca
     5701 gcaatcctct ccatcgcatc ctggaaatgg ttatttttaa tcaacgtacc gttaggtatt
     5761 atcgccctgc ttctggcgat gcgttttctg ccacccaatg gttctcgcgc cagtaaaccc
     5821 cgtttcgacc tgcccagcgc cgtgatgaac gcgttaacct tcggcctgct tatcactgcg
     5881 ttgagtggtt tcgctcaggg gcaatcgctg acgttaattg ctgcggaact ggtggtaatg
     5941 gttgttgttg gtattttctt tattcgccgc cagctttctc ttcccgtacc gctgctaccg
     6001 gtggatttac tgcgtatccc gctgttttca ctttctattt gcacatctgt ttgctctttc
     6061 tgcgcacaaa tgctggcaat ggtttccctg cccttttacc tgcaaaccgt gctcgggcgt
     6121 agtgaagtcg aaacaggttt acttctgaca ccgtggccgt tagcaacgat ggtgatggct
     6181 cctctggcag gctatttgat tgaacgcgta catgcaggat tgctgggtgc tttagggttg
     6241 ttcatcatgg ctacggggct tttttccctg gttctgctgc ccgcgtcacc tgcggatatc
     6301 aatattatct ggccgatgat cttatgtggc gctggatttg gcttattcca gtcacccaat
     6361 aaccacacca ttattacctc cgctccgcgc gaacgtagcg gtggggccag tggcatgtta
     6421 ggaacggctc gtctactggg tcagagtagt ggcgcggcgc tggtggcgct gatgctaaat
     6481 cagtttggtg ataatggtac gcacgtctcg ctgatggctg cggctattct ggcagtgatt
     6541 gctgcctgtg tcagtggttt acgtatcact cagccacgag ccagggcata aaaaaagcgc
     6601 gtcgatcagg acgcgctttt tagtatttac ttcatattac ttcaggtatt cacccgtacg
     6661 cagagcttca atacgtttat ccagcggcgg gtgagtcatg aacaactcac tgagcgattt
     6721 cgacttaccg ttaatgcaga aagccatcat gctggttgct tcttgcggtt catagctggt
     6781 tttcaggcgc tgcagcgcgg caatcatttt ctcgcgacca accagttttg ccgaaccggc
     6841 atctgcatgg aattcacgat gacgcgagaa ccacatggtg ataatgctcg ccagaatacc
     6901 aaacaccagt tccagaaccg ttgcaaccgc aaagtagatc agcgggttgc cgttgctctc
     6961 ttcaccttca tcacgatttc cgcccataaa acccgcggca agctgcgcca gaatacggga
     7021 aataaagata acgaaggtgt tcaccacgcc ctgaatcagc gtcatggtga ccatatcacc
     7081 attggcgatg tggctgattt cgtgagcaat taccgcctcg gcttcatccg ggctcatgtt
     7141 ctgcagcaaa ccggtgctga cagcaaccag agaggcatca cggcgcgcac cggttgcaaa
     7201 agcgttgatg tccggcgcat ggtagatagc cacttgcggc atagcgatcc ccgcctgacg
     7261 agcctgggtt gctacagtat tgaccagcca acgttccctt tcgttacgcg gttgctcgat
     7321 cacttccccg ccaacagatc gtaatgccat ccatttggac atcagaagcg aaacgaagga
     7381 accaccaaaa ccgaacagca aggccatgat catcagcccc tgaacgctgc tcgactgtat
     7441 ccctgtcagg ctcagtacca gcccgaaaac gaccattacg gccaggttcg ttagcaggaa
     7501 gagcgcgatt cgcatcataa ttttcttttt acctcagttt aacaaaacgc aatatgcgat
     7561 acccacatcg tatgggttac gcgactattt tcaagtctgg atagtgcgta agtcaccaga
     7621 aagacacaac tttacatttt gtagcatctg atttacggca tcttgccgct gttaaaaaat
     7681 caggcacaat ttcttgtgcc tgattgatat tacttgacgg gagcgggttg ttccgcgggt
     7741 ctggcttttt caagcttcgc cagatcgagt gcgatattca ccgtctcatc cagataagga
     7801 tccggctcct ggtaatcttt cgatagatca tccagcttct tcaactccgg tttaccttct
     7861 cgtttaaagc gttcgttcaa acgcgccaga cgcgtcgcat catcttcatt attctctttc
     7921 tcacgcacag cgtagttcag agaaacgata ttgcgcttgt ccttcatagc gttgaagcgc
     7981 gcgatatcct tcatgatgtt ctggaactca ggatctttcg cgatacgcgc attatgttcc
     8041 ttcagcagct ccggttcaaa ggcagttaaa tctcctgatt tcacataagt cgcggcatcg
     8101 atgctatccc acggcagcgc gttatcttcg aatttctcac ccgtttccgt ttcttcatta
     8161 cccgtcggca tgatgatgtc tggtgttacg cctttacgtt gcgtactgcc gccgttaacg
     8221 cgatagaatt tctggatcgt gtactgcaca gaacccagcg ctggccattc aggacgtaac
     8281 atctgatcgt aaatacggtt caatgaacgg tattgctgaa cggtgccttt accaaacgtc
     8341 ggttcaccca caaccagcgc acgaccgtaa tcctgcattg ccgcggcaaa gatttctgaa
     8401 gccgaagcac tgaaacggtc aaccagtacc accagcgggc ctttatagaa cacctgcccg
     8461 tcggtatcgc tgtcttcacg aaccttgccg ttgttatcgc ggacctgaac aatgggaccc
     8521 gcaggaataa acagacctga gagcgatacc gcttcggtca acgccccacc gccattgcta
     8581 cgcaggtcga tgatcacact gctgacattc tgtttttcca gtttctgcag ttgaactttg
     8641 acatcgtctg tcaaacccac atagaagccc ggaatatcca gcacgccgac tttctcttta
     8701 ccgacggtct tcaccgacat tttaaccgcg cggtcttcga gacgaatacg ttcacgggtc
     8761 aacgttacag tacgggtctt ggtcccttta ccagcaggta aaatttccag acgaacttta
     8821 ctgcccttcg gccctttaat taaggcaacc acatcatcaa gacgccagcc aatcacgtca
     8881 accatcggct tgcctgtttg accaacaccg acaattttgt caccaacgct gatagcttta
     8941 ctcttcgctg ccggaccacc tgccaccatc gaattgataa cggtgtagtc atcatccatt
     9001 tgcagcactg cgccaatacc ttccagtgac aaactcattt cagtgttgaa ctgttcggta
     9061 ttacgcgggg aaagatagtt ggtatgcgga tcgatttcac gcgcaaacgc cgtcattgcc
     9121 agcgagaaaa catcttcgct gttggtttgc gccagacgac gaatggcaaa tttgtagcgg
     9181 cgtgtcaggg tttcacgaat ttctttatcc gtttttcctg tcagcttcag gcttaactcg
     9241 tcgaatttga ctttactgtc ccactgcgcg ttcaactcag cctcgttttt cggccagggc
     9301 gctttgctgc ggtcaaggtt ataagtgtcg ttgccggtga aatccatcgg cttttccagt
     9361 accgacaaag cgtactggta acgttcaaaa cggcgctttt gcgccagatt gtagagatcg
     9421 tagaaaacgt cgagtttgcc tgaacgcagt tcatcgccta actcggtttt ctttttcgcg
     9481 aactgttcaa catcgcttgc cagcagcaca ttgtggctgt aatcgagcag attcaggtag
     9541 cggtcaaaga ttttggccga aaatgcctga tcgaggtcga actggcgata atgagaacgg
     9601 gtgaagcgcg acgttacgcg ctcacttacc gtcgcatgct gcgtctcttc ctttaatacc
     9661 ggaatttgat cagcacgcgt gatatcttct acagcgaagg tctggcctgc tattgcaagc
     9721 aggccagcta acgcggtaag cctaaaaaac atgttcatgc ctggcccggc ctccgtttca
     9781 gaacaccagg tgttctgcgc gcacaatcaa agacataccc gaattcagct ggacgcggac
     9841 gccgtctttg gtgatttcta atacggtggc atccatcgcg ttttgacccg ctttcacctt
     9901 cagggcttgt ccgacagtca gagctgaaat gtcagaaacc ggggtgtgct gttcttcgcg
     9961 aggtgctttt actgtttttg gcgctttctc taccggcttt tgcgcacgag gtttacgttc
    10021 agcgccttct ttgcggcgtg gcgtggtcgg acgtggcttg cgttcgcggc gcggtgcgtc
    10081 ttctttctca ccagcagctg cggcagcttc gcgttttttc gcttgctgtt cagcacgctg
    10141 tgcctgaaca cgcgctttcg cttcttcaag ctgcttgcga gcatgctcta catgttgctc
    10201 gtccagctca ccgcatgggt tgccgtcaag atcgacacgc gttgcgccgg gtttaacacc
    10261 gtaaagataa cgccagctcg aagtgtagag acgtaaagcg gatcgtaatt gcgttttgct
    10321 caggttcatt tccccggcaa cacgatcgac caaatcctga aaaataccaa ttttcagcgg
    10381 acgcgcttca ccttccgcac tgaaacagtg gggaaaacgt tcggccagaa acgcgattac
    10441 ttctttactg ctattcaact taggttgatt ttccatgaaa tttcctgatt acaacggacg
    10501 tagccaacaa gcgcaggcat gaacaggcgt cattataatg acgctaccag taaatgctac
    10561 gttatccgtt gattatcctg cgacgctcgc aaagaatttt ttgtaatccg tcgttgcaag
    10621 cactttttca agctgtgcca caagctgacg taagccttgc tcgtcctcgt ctgtaaagcg
    10681 agcgaagaca gtactatcga tgtcgagaac accaataatc tgatttttca ccaccagcgg
    10741 cagaacaatt tcagaattac tcgccgcatc acaggcaata tgcccgtcaa acgcgtgaac
    10801 atcctcgata cgctgcactt gattgcgggc aaccgctgtg ccgcacaccc cgcgcccgac
    10861 gggtatccgg acacaggcaa ttttgccctg aaatggtccg agtaccagtg tatcgtcctc
    10921 aagcaaataa aaacctgccc agttaacgtc agtgagacgc tcgtataaca acgcactggt
    10981 gttcgcaagc gttgccagaa aactggtttc tcccgccatc agcgcgttaa agtcgcgatt
    11041 taaatccgcg taaaattctg ttttgttcat tacttaatca cttggttgtc ttacctggaa
    11101 ctgccagcct attaaaataa gcattaaatg cgttaatgct caagatcatt cccatcatgg
    11161 gttaagatta atgttaattc ttattacatt tggcacgtca tggctcttaa cacaccacaa
    11221 attacgccga caaaaaagat aacagtgagg gcaatcggcg aggaactgcc gcgtggtgat
    11281 taccaacgtt gcccgcaatg tgacatgctg tttagcctgc ccgagataaa ttctcatcaa
    11341 agtgcctatt gtccgcgctg tcaggcaaaa attcgcgacg ggcgcgactg gtcgctaacg
    11401 cgcctggcgg caatggcctt caccatgctg ttgcttatgc cgtttgcctg gggcgaaccg
    11461 ctgttgcata tctggctgtt aggcattcgt attgacgcca acgtcatgca aggcatctgg
    11521 caaatgacca aacagggcga tgcgataacg ggatcaatgg tctttttctg cgttattggt
    11581 gcccccctca ttctggtgtc ctccatagca tatttatggt ttggtaaccg actgggaatg
    11641 aatttacgtc cggtactgct gatgcttgag cgacttaaag agtgggtaat gctggatatc
    11701 tacctggtcg gcattggcgt tgcttctata aaggtacagg attatgccca tatccaggcg
    11761 ggtgtcggct tgttctcttt tgtggcgttg gtgattttaa cgacggtgac gttgtcacat
    11821 cttaatgttg aggagctgtg ggagcgatat tatccgcagc gccccgctac gcgtagggac
    11881 gagaaacttc gtgtctgtct tgggtgccat tttaccggct atcctgatca gcgtggtctc
    11941 tgcccgcgtt gccatatccc gctacgcctg cgtcgtcgtc atagtttgca aaaatgctgg
    12001 gcggcgctgt tagcgtcaat cgttttgttg ttaccagcca acctattgcc tatttctatc
    12061 atttatctga atggtggccg gcaggaagat acgattcttt ccggaattat gtcgctggca
    12121 agtagcaaca ttgcggttgc gggaatcgtg tttatcgcca gtattctggt accgtttact
    12181 aaagtgatcg tcatgttcac tttactgttg agcattcatt ttaaatgcca gcaaggttta
    12241 cgcacacgca ttctgttact gcggatggtg acctggattg gacgctggtc gatactcgac
    12301 ctgtttgtca tatctttaac catgtcgctg attaatcgcg atcagatcct cgcttttact
    12361 atgggaccgg ctgcgtttta ttttggcgct gccgtaattt tgactattct tgctgtggaa
    12421 tggctggaca gccgcttact ttgggatgca catgagtcag gaaacgcccg cttcgacgac
    12481 tgaagcgcag attaaaaata aacgccgtat ctcacctttc tggctgctgc ctttcatcgc
    12541 gctaatgatt gccggttggc tgatttggga cagttatcag gaccggggta ataccgtcac
    12601 catcgacttt atgtcggcgg atggtattgt tccgggccgt acgcctgttc gttatcaggg
    12661 cgttgaagtc ggaacagtgc aggatatcag cctcagcgac gatcttcgta agattgaagt
    12721 caaggtcagc atcaagtccg atatgaaaga tgcgctgcgc gaagagactc agttctggct
    12781 gatgacgcca aaagcatcgt tggcaggtgt ctccgggctg gacgccctcg tcggtgggaa
    12841 ctatatcggc atgatgccgg gtaaaggtaa agagcaggat cactttgtcg cgctcgatac
    12901 ccaaccgaaa tatcggctgg acaatggcga tctgatgatc cacctgcaag cccccgctct
    12961 cggttcgctg aacagcggtt cattggtcta tttccgcaag atcccggtgg gaaaagtcta
    13021 cgactatgcc atcaatccca acaagcaagg cgtggtgatt gatgtcctga tcgagcggcg
    13081 ttttaccgat ctggtgaaaa aaggtagccg tttctgaaac gtttccggcg ttgatgccaa
    13141 cgtcagtatc agtggcgcga aggtgaaact ggaaagtctg gcggcactgg ttaacggtgc
    13201 gattgccttc gattcacctg aagagtcgaa acctgccgag gcggaagata cctttggtct
    13261 gtatgaagat ctggcccaca gccagcgtgg agtaataata aaactggaac tgccgagtgg
    13321 agcaggatta accgccgact cgacgccgtt aatgtatcag gggctggaag tcggacagct
    13381 gactaaactg gatttaaatc ctggtggtaa agtcactggg gaaatgaccg ttgatccctg
    13441 cttcgtgaaa atacccgcat cgaattacgc aacccgaaat tatcccttag cgatgctaat
    13501 ctcagcgccc tgctgaccgg aaaaaccttt gagttggtgc ccggcgatgg cgagccacgc
    13561 aaagagttcg ttgttgtgcc gggcgaaaaa gccctgctgc aagagccgga cgttctgaca
    13621 ctgaccctga cagcaccgga aagttacggt attgatgcgg gtcagccgct cattcttcac
    13681 ggcgtgcagg taggccaggt tatcgatcgt aaactcacca gcaaaggcgt cacctttacc
    13741 gtcgccatcg agcctcagca tcgagaactg gtaaaaggcg atagcaaatt tgtcgtcaac
    13801 agccgtgtcg acgtgaaggt ggggctggat ggcgttgagt ttctcggtgc cagcgcctca
    13861 gaatggatta acggcgggat acgtattctg ccgggcgata aaggcgagat gaaagccagc
    13921 tatccactgt atgccaatct ggaaaaagcg ctggagaaca gccttagcga tttacccacc
    13981 acaaccatga gtttgagtgc agagacgctg ccggatgtgc aggcaggatc ggtagtgctg
    14041 taccgtaaat ttgaagttgg tgaagttatt accgtgcgtc cgcgagctaa cgcgttcgat
    14101 atcgatctgc acattaaacc ggaatatcgc aaccttttga ccagcaacag cgtgttctgg
    14161 gcagaaggcg gggcgaaagt tcagctgaat ggtagtggcc tgaccgtaca ggcatccccg
    14221 ctctccagag cattaaaggg ggccattagc ttcgataacc tcagtggtgc cagcgccagt
    14281 cagcgtaaag gcgacaagcg aattctgtat gcttccgaaa cagcggcccg tgcggttggc
    14341 aggcagatta cgcttcacgc tttcgatgcc ggaaaactgg cggtcgggat gccaattcgc
    14401 tatctcggta ttgatatcgg gcaaatccag acgctggatc tgattaccgc acgcaatgaa
    14461 gtacaggcaa aggcggtgct ctatccggaa tatgtccaga cctttgctcg cggtggtacg
    14521 cgcttctcag tggtcacacc gcaaatctcg gcagctggcg ttgagcatct agatactatc
    14581 ctccagccgt atatcaacgt cgaaccaggc cgtggcaatc ctcgtcgcga ctttgaatta
    14641 gaagaggcca ccattactga ttcgcgttac ctagatggtt taagcattat tgttgaagcg
    14701 ccggaagccg gttcgttagg catcggtacg cctgtgctgt tccgtggtct ggaagtaggt
    14761 acggttaccg gaatgacgct ggggacattg tcagatcgcg tgatgattgc gatgcgcatc
    14821 agtaaacgct atcaacacct ggtgcgtaac aattccgtct tctggttggc atcgggttac
    14881 agtctggact ttggtctgac gggcggcgta gtgaaaaccg gcacctttaa ccaatttatc
    14941 cgtggcggca tcgccttcgc cacgcctccg ggtacgccac ttgcaccgaa agcccaagaa
    15001 ggcaaacact tcctgttgca ggaaagtgaa ccgaaagagt ggcgtgaatg gggaactgcg
    15061 cttcccaaat aatgcccact gctccggcgt gcctgcgccg gagcgcttat gctaaactgc
    15121 gcgcctgttt ttttgccagt ggtacatgct cgtggcccaa cacaccgttt atttcccgga
    15181 cgcgtttctg acacaaatgc gcgaagctat gccttcgacg ctctcatttg atgattttct
    15241 tgccgcctgt cagcgcccgt tgcgccgcag cattcgcgtt aatacgctga aaatctccgt
    15301 tgctgatttc ctgcaattaa ccgctcctta tggctggacg cttacgccaa ttccgtggtg
    15361 tgaagaaggt ttctggattg aacgcgacaa tgaagatgca ttgccattgg gtagtaccac
    15421 cgagcattta agtggcctgt tttatattca ggaagccagt tcaatgttgc ccgtcgccgc
    15481 cttgtttgct gacggtaatg caccacagcg ggtgatggat gtcgctgctg cgccaggctc
    15541 aaaaacgacg caaattgccg cgcggatgaa taacgaaggg gcaatccttg ccaatgagtt
    15601 ttccgccagt tgggtaaaag tgttacatgc caatatcagc cgctgtggca tcagtaatgt
    15661 tgcgctcaca cattttggtg gccgcgtgtt tggtgcggca gtgccagaaa tgtttgatgc
    15721 cattttgctg gacgctccct gctccggcga aggcgtggtg cgtaaagatc ccgatgcgct
    15781 aaaaaactgg tcacccgaaa gcaatcagga aatcgcagct acccaacggg aactgattga
    15841 cagcgccttt catgcattac gtcctggcgg tacgctggtt tactcgacct ataccttaaa
    15901 ccgggaagaa aacgaagccg tttgccggtg gctgaaagag acttaccccg acgaagtaga
    15961 gtttttacca cttggcgatc tcttccctgg tgcaaacaag gcgctgaccg aagaaggttt
    16021 tttgcatgtt ttcccacaaa tttacgactg cgaaggcttc ttcgttgctc gtctgcgtaa
    16081 aactcaggcg atccccgcct tacccgcccc aaaatacaaa gtgggcaatt tcccgttcag
    16141 cccggtgaaa gatcgcgaag ccggacaaat tcgtcaggcg gctgcaagtg ttggcttaaa
    16201 ctgggatgga aacctgcgac tctggcaacg cgacaaagaa ctgtggttgt tcccggtagg
    16261 cattgaagcc ctgatcggta aagtccgatt ttctcgctta gggattaaac ttgccgaaac
    16321 gcataacaaa ggttatcgct ggcagcatga agcggttatt gcccatgcct cccccgacaa
    16381 tgtaaacgct tttgaactga caccgcagga agcgtaggag tggtatcgcg ggcgcgatgt
    16441 ttacccgcaa gccgcgccag tggcggatga cgtgttggtt actttccagc atcagccgat
    16501 tggtttagcc aaacggattg gttcgcgact gaaaaacagc tacccgcgtg aactggtgcg
    16561 ggacgggaaa ctttttacca gtaacgcatg acagcgcaca aaaaaagcgc actttttgac
    16621 tggcacattc gccggcctca actaggctga aaaattgtgc gatcggactg gtcgtactac
    16681 aatcagcagc taaatggaga gcacgaagat gaaaaccagt gtgcgcatag gcgcttttga
    16741 aatcgacgac ggcgaattac acggtgaatc gccgggtgat cgaacgttaa ccattccttg
    16801 taaatctgac cccgatttat gtatgcaact ggatgcctgg gatgctgaaa ccagtatccc
    16861 tgccctgcta aatggcgaac actctgtcct ttaccgtacc cgttacgatc aacaatctga
    16921 tgcctggatt atgcgtcttg cctgatccaa aaagaacccg tcggcatggc gggttatttg
    16981 tcctggttat tcccccgttg taaaatctct cctaaactta acggtacggc accacacttc
    17041 ggggatgaaa tgttcgcgct ggtacttttt gtttgctacc tggatggcgg ttgtgaagat
    17101 attgttgtgg atgtctacaa cacggaacag cagtgtcttt attctatgag cgatcaacgg
    17161 atccgccatg gcagttgttt tccgattgag gattttatag atggtttctg gcaaccagca
    17221 caggagtatg gtgattttta attattgcag ttgcacaaga gtcagttagc ccccaaagac
    17281 agcaccggta tcaatataat gcaggttgcc aatatccacg cgatgtcgca acggtgtatg
    17341 accaaaccag aaatgatcag cacctgtaat tccctgccct tttgggcgtt cacctaatcg
    17401 cgagcggctc cacaagacct gatgcaaatc aacgtccttt tgccattcat a
//
LOCUS       DAZXUL010000092        17423 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZXUL010000092 DAZXUL010000000
VERSION     DAZXUL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17423
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.092"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(139..3654)
                     /locus_tag="ACH3L1_002980"
     CDS             complement(139..3654)
                     /locus_tag="ACH3L1_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFT4077637.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="ACH3L1_002981"
     CDS             complement(4107..5369)
                     /locus_tag="ACH3L1_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4077638.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="ACH3L1_002982"
     tRNA            complement(5567..5642)
                     /locus_tag="ACH3L1_002982"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="ACH3L1_002983"
     CDS             complement(5748..6455)
                     /locus_tag="ACH3L1_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFT4077639.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6608..6691
                     /gene="yqgH"
                     /locus_tag="ACH3L1_002984"
     CDS             6608..6691
                     /gene="yqgH"
                     /locus_tag="ACH3L1_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFT4077640.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="ACH3L1_002985"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="ACH3L1_002985"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFT4077641.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="ACH3L1_002986"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="ACH3L1_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFT4077642.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="ACH3L1_002987"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="ACH3L1_002987"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFT4077643.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="ACH3L1_002988"
     CDS             complement(11639..11914)
                     /locus_tag="ACH3L1_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFT4077644.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="ACH3L1_002989"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="ACH3L1_002989"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFT4077645.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="ACH3L1_002990"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="ACH3L1_002990"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFT4077646.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="ACH3L1_002991"
     CDS             13874..14200
                     /locus_tag="ACH3L1_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFT4077647.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="ACH3L1_002992"
     CDS             14384..15103
                     /locus_tag="ACH3L1_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFT4077648.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="ACH3L1_002993"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="ACH3L1_002993"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFT4077649.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="ACH3L1_002994"
     CDS             16442..17404
                     /locus_tag="ACH3L1_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFT4077650.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
BASE COUNT         4439 a         4261 c         4338 g         4385 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aac
//
LOCUS       DAZXUL010000093        17092 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZXUL010000093 DAZXUL010000000
VERSION     DAZXUL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17092
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.093"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..510
                     /locus_tag="ACH3L1_002995"
     CDS             <1..510
                     /locus_tag="ACH3L1_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFT4077651.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACH3L1_002996"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACH3L1_002996"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFT4077652.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="ACH3L1_002997"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="ACH3L1_002997"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFT4077653.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="ACH3L1_002998"
     CDS             complement(2878..3075)
                     /locus_tag="ACH3L1_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFT4077654.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACH3L1_002999"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACH3L1_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFT4077655.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACH3L1_003000"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACH3L1_003000"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFT4077656.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACH3L1_003001"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACH3L1_003001"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFT4077657.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACH3L1_003002"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACH3L1_003002"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFT4077658.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACH3L1_003003"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACH3L1_003003"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFT4077659.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACH3L1_003004"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACH3L1_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="ACH3L1_003005"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="ACH3L1_003005"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFT4077660.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACH3L1_003006"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACH3L1_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFT4077661.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="ACH3L1_003007"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="ACH3L1_003007"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFT4077662.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="ACH3L1_003008"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="ACH3L1_003008"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFT4077663.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="ACH3L1_003009"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="ACH3L1_003009"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFT4077664.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACH3L1_003010"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACH3L1_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
BASE COUNT         3993 a         4865 c         4443 g         3791 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc ctcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct agcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta at
//
LOCUS       DAZXUL010000094        16362 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZXUL010000094 DAZXUL010000000
VERSION     DAZXUL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16362
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.094"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..255
                     /locus_tag="ACH3L1_003011"
     CDS             <1..255
                     /locus_tag="ACH3L1_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077665.1"
                     /translation="WRFLPLPDRAKHKQKKARNYAGLAAFHAIRCLAVPDAESFLPDM
                     LINFALINITDRKNEGTNTIDGNWQADEGRRYRDNVRIYF"
     gene            complement(353..784)
                     /locus_tag="ACH3L1_003012"
     CDS             complement(353..784)
                     /locus_tag="ACH3L1_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFT4077666.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(852..3563)
                     /locus_tag="ACH3L1_003013"
     CDS             complement(852..3563)
                     /locus_tag="ACH3L1_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFT4077667.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRIRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVELGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPRMGFEPEGSYQQFNDVFAEMVAWHNAAGITGSGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(3575..3907)
                     /gene="mobA"
                     /locus_tag="ACH3L1_003014"
     CDS             complement(3575..3907)
                     /gene="mobA"
                     /locus_tag="ACH3L1_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFT4077668.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            4140..4475
                     /locus_tag="ACH3L1_003015"
     CDS             4140..4475
                     /locus_tag="ACH3L1_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFT4077669.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     ILVRTKGYTLTDVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(4561..5409)
                     /locus_tag="ACH3L1_003016"
     CDS             complement(4561..5409)
                     /locus_tag="ACH3L1_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFT4077670.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRMDKTEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            5990..6241
                     /locus_tag="ACH3L1_003017"
     CDS             5990..6241
                     /locus_tag="ACH3L1_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFT4077671.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(6272..6517)
                     /locus_tag="ACH3L1_003018"
     CDS             complement(6272..6517)
                     /locus_tag="ACH3L1_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077672.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEV
                     AREIACRLRDIGWPLERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(6517..7443)
                     /locus_tag="ACH3L1_003019"
     CDS             complement(6517..7443)
                     /locus_tag="ACH3L1_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFT4077673.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGALEKAL
                     AIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(7440..7829)
                     /locus_tag="ACH3L1_003020"
     CDS             complement(7440..7829)
                     /locus_tag="ACH3L1_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFT4077674.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            complement(8006..8272)
                     /locus_tag="ACH3L1_003021"
     CDS             complement(8006..8272)
                     /locus_tag="ACH3L1_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077675.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(8365..8799)
                     /locus_tag="ACH3L1_003022"
     CDS             complement(8365..8799)
                     /locus_tag="ACH3L1_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFT4077676.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            complement(9532..10032)
                     /locus_tag="ACH3L1_003023"
     CDS             complement(9532..10032)
                     /locus_tag="ACH3L1_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFT4077677.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(10494..11090)
                     /locus_tag="ACH3L1_003024"
     CDS             complement(10494..11090)
                     /locus_tag="ACH3L1_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077678.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            complement(11087..11806)
                     /locus_tag="ACH3L1_003025"
     CDS             complement(11087..11806)
                     /locus_tag="ACH3L1_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFT4077679.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            complement(11803..12237)
                     /gene="psiB"
                     /locus_tag="ACH3L1_003026"
     CDS             complement(11803..12237)
                     /gene="psiB"
                     /locus_tag="ACH3L1_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFT4077680.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWVMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(12292..14250)
                     /locus_tag="ACH3L1_003027"
     CDS             complement(12292..14250)
                     /locus_tag="ACH3L1_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFT4077681.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYAVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDARAYRNLPEPEAVLTEAED
                     ERLNELMARYDSLENQCEESDLLAAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CIQRGVQLRSEDDVADDADRTEQGLESASVEEISLPLLTKLTSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSKAHSSPARIRLECEHYALTSDAPSGKEGAAFMALMAEHSRF
                     AALLPEGWERDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCAPRPQTDATEHTANLADAA"
     gene            complement(14309..14542)
                     /locus_tag="ACH3L1_003028"
     CDS             complement(14309..14542)
                     /locus_tag="ACH3L1_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFT4077682.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNQYIRDFGILK"
     gene            complement(14600..15127)
                     /locus_tag="ACH3L1_003029"
     CDS             complement(14600..15127)
                     /locus_tag="ACH3L1_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFT4077683.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            complement(15480..15761)
                     /locus_tag="ACH3L1_003030"
     CDS             complement(15480..15761)
                     /locus_tag="ACH3L1_003030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077684.1"
                     /translation="MQGTGVIFRDTVTVSGCLLLRPLRLPAAWRHPVVRPDSDGQLLA
                     HPPVSAEPAGAVSGCRYGPMRENPGLQRPFRSRRPCRAVTCAPCTPDGV"
     gene            complement(15899..16090)
                     /locus_tag="ACH3L1_003031"
     CDS             complement(15899..16090)
                     /locus_tag="ACH3L1_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077685.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(16087..>16362)
                     /locus_tag="ACH3L1_003032"
     CDS             complement(16087..>16362)
                     /locus_tag="ACH3L1_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077686.1"
                     /translation="VMQRLDDAFVYGACDRVVSDIVNELMEEKRASRHVTVPAVMLEK
                     VMALAGSEMKRLYAVGSENGGDGDAFVREELEAMDVVLQALDGETMS"
BASE COUNT         3679 a         4823 c         4123 g         3737 t
ORIGIN      
        1 tggcgcttcc tcccgttgcc agatcgtgcc aaacacaaac aaaaaaaagc ccgcaattac
       61 gcgggcttag cggcatttca tgccatcagg tgcctggctg tgccagacgc ggaatcattc
      121 ctgccagaca tgctgattaa ttttgccctt attaacatca cagacagaaa gaacgagggt
      181 accaatacca ttgatggtaa ctggcaggcc gatgaaggac gccgttaccg ggataatgta
      241 agaatctatt tttgagtatg tcaacataac ctgaggcaaa gtacctcagg ttccagtgta
      301 gtaatcagcc aacctgacag tcttctgtta gccctacaga tatgtcgtgg agctaaccgc
      361 gcatgtcaaa gccggatttt tgaaaatctc tataaacttc cggattgttg aaggccggga
      421 atttagcttt atgggctgct gactttatcg cttcgcaata ggctttatcg ccattactgg
      481 ttgatatttt taacgccgtg ccatcccggg agaattccat atgcaacctg cattttttcc
      541 ttttccattt ttgcggatca tcaagtttag cattaattgc atccctgatt ccccgtgctt
      601 gctcccccca ttcatcctga tcatcccaac gtcctgaact gcaactaccc agggctgtag
      661 ttttatggca atttgaagga tgtaatggcg cacatcccgc cacaaagctg actaaaatag
      721 ccagcataac gattttcttt acccccatat attgctcctc aatccatata gatctcagta
      781 ccatagctct gtcagagcaa agaaaatctt aactaataca agttaagatt tttaatatcc
      841 actcctcatc cctacattcc aggtgatggt ggcgtccact cattatcctg aactggagca
      901 taagatgcgc catgcccccc ggatatgctt ttctgttgcc tgatatattc gtggtagtac
      961 tgttcactac gctgacgatg taaatccaca tccgggcggc tgattacaaa gtgtccactt
     1021 ccggttattc cagctgcatt atgccaggcc accatttccg caaatacatc attaaactgt
     1081 tgatatgagc cttccggttc aaaacccatt cttggctcac gatcaaacaa tacaggagca
     1141 atcagattca gcttatccac cagttcattt ctgtcctgat gatggtagaa caccacacgg
     1201 tccccataat ccacacagac taactcgcca ttcctccgat cacgaaaacg cacactgcca
     1261 tctttctgca gacgggcttc gagaacgcca gtgtcttcat aaagtggagt tcctcccggt
     1321 tcacagagaa tcacgcagcg gtcggcattc gttgttcgtc gcttgtcttt gcgacgatca
     1381 cgataatccc agccacgtaa ctgtgataat gcagctctgt ctccctgtac cgcctgttgc
     1441 tccacccact gccggtagga caaaggatac cacttaccct ccgcaatcag tgacaaccgc
     1501 tcttccttca cactctcctt cagcctgatc agcgcctgca tacgctgcac ttctgcaata
     1561 tggtaatgca gcttacgcaa ttgtggatcc cggaactgca cacggatata cgccttgcgc
     1621 cgacggcagg cagcatgaat ttcccgtaac cgttctccgt aacgtaagtc gggtttacgc
     1681 cagtgctcct tccatgcaag ataacgcgcg cgcaaatctt cccgcgcagc tgcacgggct
     1741 tcacggcgtt cacgacgcag tacaggatcg cgccggaagc cgagctccac ttcatcgctg
     1801 gcagcaagtt ccggattgta gcgacattca ggcttcaccc gttcaaatat atctgcacct
     1861 gcaatctcaa atggccctgc ctgcggctca gccctggata acgtcaaatc cgggtggata
     1921 ctgctggcct tcactggtgt gagctcatga ttaaacgcat ccacaatcac aagaccatga
     1981 tgctgtttct gcaacataag ccctttacgc gcaaacatct catggcacgc ctgccagctg
     2041 aaattcccgc ccgcgatcat atcttcaatc aacgactcac actgaacagt cgtgtacccc
     2101 cacagacttt ccttatcgga aaacagttcg cgctttattg ctccctgtgg acctgtcccc
     2161 atccgaatac gataccattc gtaatcactg acagcctgag taccaagatt acgtccccgg
     2221 aatcgttctc cgggtataac ctgagtaaaa atatccttcg gtacaggctg ccatccacca
     2281 tccagatcct gcatataatc cagcgtcatt aacggccaca cactgtctgc acgtactggt
     2341 gtctgttgtt tatcaaatcc atcatgcagt accagatgcc cgtgctggat ccccatccat
     2401 aatccagcct ttgccagagt tctgtgcaca tccaggcaac tttccagctc accatttctc
     2461 gccatttccg gtaagcgttc ggcaaaatga cggcaggcgt actgtttcag actctccgtt
     2521 tctgccactt tttccggccg gatatcaacg tttatggctt ccggatcata acgaccgggt
     2581 gtccccacct gactgaaaat atctgtcggt acaggctgat attcccccag tttccgcccc
     2641 agtttttcag ctgtccatga cggtccgaac gaactgagtg caacaccttc ccgcgctctg
     2701 tcccagccat ccttcaccac cagttcccct tcctgcatcg tgatatacag accatcactg
     2761 gccagccgtt tatgcaaaga caaccagtct tgtgcgggct cttcacgcag cccggcaatc
     2821 gacatctgcg caatatattc cctgaacgtc tgctttttcc cgcgtcgcca ggcattacgg
     2881 cgctcacgta ccagcgctgt tttgcgcaca atacggttgc cgggtgcatg cacaaagcag
     2941 ccattatcag gcgcgaatcc gtgcttaagt tcaagttcac gacatgcacg gctgagtttt
     3001 tcctggctcc aggaaagcca gttaatataa ccagtttcag gatgtacacg attcacggca
     3061 acatgcacat gcagattatc cgtgtccgta tgaacggcag ccacgtactg atgttttgag
     3121 agttcaagag atttcagtgt gtgacgcaca cagtcaaaaa gctgctcagg acgcgggctt
     3181 tcatgcgcag gccaggaaag aatgtagtgg aaaacggggt ctgtatcatt acgggaaaaa
     3241 acagctttgt ccgccttgta ctgcatctct tcagcagctg tttccagtga attgcagtta
     3301 tggaaacagg ttacgccata gaaatttacc cactgactgc cgtccttcat cacatcaatc
     3361 agggagacaa atttttcgtt acgcaggcgt gttgcataat caacaagccg tctgaaacgg
     3421 ttgcggtgag ggacatcact cttgatttcg ctgtcttctg tgagctcatt ttcctgcaca
     3481 tcatcccgca cagacgtgta cgcaatcagg tcttcaaagg aagacctgcc gtcacgtctt
     3541 ttgtgcggaa taatggcatt catcagcagt tatctcagcg tcccattaag tctcttcgca
     3601 gagcattgac atattcagta atggcaacaa gaacctctgc atactcctta tcaccagtgc
     3661 gttttccctc cacaaacaga tgcttctgaa ggcggccaag acgcataatc tcatcaatca
     3721 tacggtcgtc agtacggcag ttaaccacct tatccagtga agaacttcgt ataaatgttg
     3781 aaaccgtctg tccacttgac tccgcttttt ctcttacgat ttcagcctca tcctccgtga
     3841 atctcgccgt gaattttaca attctcttcc tgttctcact gcccgttcgg gttttcttct
     3901 cactcatggt ctgctcctta ccggggtgtc ggggcaaagc cctgaccagg tggggaatgt
     3961 ctgagtgcgc gtgcgcggtc cgacattccc acatcctgtc ccgtttttca gtccattata
     4021 gcctgttatt tttgtgcatc atagtcttat gcagacggca gaggcagggc gcagccctgc
     4081 caggagtcag tttttgatga taaaatcagg cttcaacatt aaacggagag aaagtgccaa
     4141 tggcgtctaa aaaattttac tcagatgatg acatccagct ggcaaaagct gctttatcgg
     4201 aattgccaga cctcaccgct caacggaaaa cattgcgtga ttttcttgat gccattcgtg
     4261 atgacatcat tatcctggta aggacaaagg gttatacact gacagatgtt cgtgacacgc
     4321 tcaaaaatgc aggatatgaa gtaggagaga aagcacttcg tgacattatt cgggaagcag
     4381 aatcgaaaaa accatcccgc cgttcttcct ctaaaacagc acctaaaaag acagactcag
     4441 gcaggaaaga tggcatcgat atgaacaaca attgatgtga caatgtcaca ttgtgttctg
     4501 agattatgat gattacagaa aaaaaagccc cggcataaat acctgggctt ttctgtttaa
     4561 tcagactcgt ggcggcaatg caccaggata atgccgtgca ataatatcat tcagtttttc
     4621 aacaaggtca gaacgcttaa agacaatgtg tacggtccct ttctggtagc cacgcaccgt
     4681 aaaatattcc ccttctaaca gcttaccgac attgtcaccg ttcccgagaa attcaccata
     4741 ctgcgccccg atgctgttcc tgaagtcagg aacgttgcgc ccctccagca gataaaacgg
     4801 acgtaccagg tcatccagtg cctcccgtcc gctgcagtcc agcgtcacac gcccgttact
     4861 ccagcggtac agtaggtttt caataattat tttcttaccc agacggcagg gattattggt
     4921 tctgtaatcc catgacaatt tccggtagac atcaatcagc ccctgttcaa acgtctgcat
     4981 cttgctggca ttaagatggc ggaatgttgc cagtacattg tccagggtga tttcaggaca
     5041 ggtgtcgctc atcagctggc tgttccactc atcacgctgg catgaactca tgaaggtgta
     5101 catgcccgta tctgtcatca ggcggtccca gattttccgg tcggcaagac gggtgatatt
     5161 ccttttcgct tcttccgtct tatccatgcg acgaacggaa tcagtcacgc agtcttcaaa
     5221 cccgtaaaga aaaatatcac ctgacgcatc gagcagaatc ttgcgcgctg tcgccaggca
     5281 ttccatcgcc tgcatgaaca gtgcaatacc ttcgttacgc tgcgcgatga tgcggtcgat
     5341 agctacagac ggaataacat ctgtcgtgcc ggcagtattc aggtcagcag tgggtaaagt
     5401 ctgattcatc acattgtctc ctcatcgtca cgactgccgg gtcagccagg tcatttcccg
     5461 gaggagcgaa gctccttctg ccgtccgggt cttcggggat ttccccgcag tggacctttc
     5521 cgacggggga cgctcaccac gtcacggggc agttgtccgg gggcagacgc aaaagttttt
     5581 gacgcctgcg gcaaaaagtt ttgtggctgg tacatctctc ccctggacga ctgaaacggg
     5641 atgtggtcat gtatgtaccc ccggaaagtc cgtctgcgga gaacaggccg cagtgcggcg
     5701 gcagacaggc agagacagct gcgccatcag gcgcagtggc cttacacacc gtaaggtgtg
     5761 cagggagcgc cgcatacggc gcgactgaag aaaaacgacg gcacaacaat ggcatgtggt
     5821 ggtcactggc acaccactcc cgcacaggtg aaaacagaga aaaccgcacg acatttacaa
     5881 ccaaaaaaaa gtgaaatttc ttcacttttt cacgcttatt tttcgcgttg atttggtgat
     5941 tatgatgata aaataaagtg aaattttttc actttttcaa aggtgtttta tgaccggctg
     6001 ggaactgcga atctggcgaa aaagcatgct ctggtcacgc gaaaaagcgg ccagagaatt
     6061 tggcgtgacg cagagaacat ggcacgcctg ggaaaatgcg gaacaggtgg acgtcaccgt
     6121 ctggaggacc acccaggcac tttcagtccg tgacctgctg cctcacatgc aggggatgcg
     6181 taaggcagat atcatcaggc gactggaaaa tgaactgggg gaaacggcag ggaatgtgtg
     6241 agcgtgattc acagcctggc tgaatgccag gctactgctc atcaggaaac agctttttca
     6301 gttcttcacc ggaaagtccc gtgacctcct gaatccgttc cagcggccag cctatatccc
     6361 gaagccggca ggctatttcc cgcgccactt caagagcccc ttgtttaaaa ccctcgtcga
     6421 aacctttctg tataagacgt tctgcaatcg tcatgctgtt tgctctgctc ctgctgacaa
     6481 aaaagcccgg cattttgccg ggctttgtgg ttctcatcag tgagtgattt tcttcagttc
     6541 gtcatcggaa agtccggtag cctgcttaat ctgctccggc gtcatcccca ttttctgaag
     6601 ctggcacgca atagccaggg ctttttcaag cgctccttcc atgcgtcctt ccatgcgtcc
     6661 ttccatcctg ccttcctggc gacctttttg ctcaagacgt tctgcgatag tcatcagttt
     6721 atcctcatgc tgcggcagac gctcagccag cgcccgcata aattccggtg atacagagtc
     6781 ccctgcgtta acgatgtaat gaatcagaac acgcacctgc tcgtctgaaa tttccaccat
     6841 gacctgcggg agtttgtcca gcagctccat catgtcacga tggcggatat gcttcatcag
     6901 cagcgtcagt gcggccatcc ggcgatggtt catgatttca ttgtcatcaa cgacagtgat
     6961 atcaaccagc ggaaacggct ttgtgtatat tttagccgca agtgccggat tctcaaaaca
     7021 gtccagccag ttcatgctgt acgggtacgg gctgcgctca ccgtgataga acagcaccgg
     7081 aatcaccagt ggcaacgttt tgtgctgctc cagatgtctc tgcatcgccg ccacactgta
     7141 gcgtaacatg cgaaaaggca tgtacagatt ttctgtgctc tggtgctcga tgagcgtata
     7201 aacataacca tcttcacccc cggtggtttt cacgctccag aggatatcgc tggcgtactg
     7261 atgcaggtcc ggctcaacaa aggtggcggg ttccagcttc agcgtggaca ggtcgcacaa
     7321 ctgccggtac tccgcaggaa gatgcagttc cagaaaatcc ctggcaacgt cgggattcgc
     7381 caggaacgac cggaacgcgg catcatgcgg cgtgggcgtt gtggtgtttt tcttcttact
     7441 cacggttcct ccatcaaatc agcagcatcg cagcaatcat cgcccaaatc cccacaaccg
     7501 agaggacagc aattgcgttc aaagccatga caacaatagc cgcgttctga gtcacggcgt
     7561 atttcttcag caatctcttc tgcggaaagg taatcaaact cctcacgggc cagttccagg
     7621 accagtagat gttgttctgt cggcaatgtg gccacaaaat tatcaatagc agcactcaaa
     7681 cgtcgctgat atagctcata aacaagacca caacggggca ttagttcttc ggataatagt
     7741 tccagttttg agcgaattct gtcagcgttg aaagtgactt catcaaaaga catatgaaaa
     7801 gctttgtcag tatcaaaaca agacttcatt gttatctcca gccctcccgg gccctgaatg
     7861 aaagagaatc tgtttccctt ttctgttttt tgtggagaca ccatgcaaca cagacgtcgt
     7921 gatgtttttc atcttgctca cggttcctcc tggtaaagta cctgaacaca tcctgccacc
     7981 tgcacaggca gacggcaggg gttaatcagt gacagacccg ttcaatttca agcgcgcgga
     8041 gcaggtggcc ccattcgctg actttcatgg cggggatacg tacccgcttc tgctcactgc
     8101 agcgtctggc gatatgaccg cccatcgcca ggacgtccgg gttaatctca tccggtgcca
     8161 gttcctggcg tctgaagcgg gtatggcggt gcaggggttt gttctcccgc aggcactctt
     8221 cacgcagacg ctttacatac tcggcggttg cgaagataat tgatggtctc atggcataat
     8281 ctccatagtt agtgaatgag atgccggact tccgtacggg gaagcgccag cggagaaagg
     8341 cagggtttat cgccctgctt tttttcattc cggacagcga cgcagtgcca gcgcgagcgc
     8401 tttcatcttc cagccttccg gcgtgcggta catcaccttc cagtagccgt cgatgaacac
     8461 ataatggaag cgcacctctt cagtgctttc cgtggcgatg gcctccctga aagaacggta
     8521 ctcacgcgcc tcactgtcca tccggaaacc actgtcacgt ccgtaataag ccgtcacacc
     8581 gtccaccggg ttctgaagcg tatgcccggc gggtttgtcg caacggggac cgaggcaacg
     8641 gatatcaccg tgttcaacca gggcttcggc ctgctgaccg gtggcatagt gggtatgcag
     8701 gatgcgaccg ttccaggtcg gctcaccgtg caggtggcag taaacggtac ggaacacccc
     8761 gtcgcggcaa aaaacggaaa tcgtgctgct gactgtcatg aaaacgtctc cttctgctga
     8821 atcaggtcgc cgggccgacc ggttgctccc ccggaggggc gaaagcccct tctgccgtcc
     8881 acttcttcgg ggtttctccc cgcagtggac ctttccgatg gggcagcgac tgccacggca
     8941 cggggcagtt gtccgggggc agacgcaaaa gtttttgacg cttgcggcaa aaagttttgt
     9001 ggctggtaca tctctcccct ggacggctga aacgtgttgt ggccgcttca gcccccggaa
     9061 agtccacagc ggggaaacag gccgcagggg gggcggcgga agggcagaca actgcgccat
     9121 caggcgcagt ttccttacac accgtcaggt gtgcagggag cgcaggtcac ggcgcgacag
     9181 aggcggcgcg accggaacgg tcgctgcaag cctggttttt cagacatcgg accgtagcgg
     9241 cagcccgaaa ccgcgccagc gggttcggcg gagactggcg ggtgcgccag cagctgcctg
     9301 tcggagtcgg gcctgacgac cggatgccgc caggcagccg ggagccgaag ggtgtccccg
     9361 cccccgggag tcagaacaac agaaacgaca aaacagaaaa atgcctcaca ctatgacagg
     9421 caggaagggt agcgccgcag gcgcggccct cctgtatttc aggcaggcgt aagcctgcca
     9481 gggttttccg ggggcctggc aggccatccc cccgccggag cggggagtaa atcaccgacg
     9541 gaacacatga ccgtcaataa aggtgaagga gtccaggaat aaatcccgcg catacgcctc
     9601 atagtcaaaa tagagcgcca ccgtctccgg cacgtcagcc agcaggccgg tatcactgac
     9661 gaactcaacc gcaaaagcct cctcactgtc agcctcgccc caccaggcat cgcggaagct
     9721 gtcaaaatcc gtctcaccgg tatcatccac ccagagacga taagcctctt cgcagccttc
     9781 atcccgcgcc tggcggaagc cttcaaccca ggcccagttg atatggcatt cagaggccat
     9841 attccccggg aatccctcat aatcctgaaa catcagttcc gggtcggttt catcctggtg
     9901 aagggcacgg caggcggcga aaaagtcgcg ttcatcatca aacgttgtca ggtcaaacca
     9961 gcggccagcg atgcttccac agttgtattt gtgccaggtt ccaacgtata cagcaggtgc
    10021 aacaacagac attcccgaat cctccatagt cacatgttct gcgacaccgg gcggtgccgt
    10081 gcctccatct ttgccgggcc tgcagggatg caagggcgca agggaagccg cagcccgacg
    10141 cacacggctg gcgggagttg gagggtgaga ccgcgaaatc tgagcgggtg aacgaccgga
    10201 aaatcacggc agacgggaag gagggaccgg gtgaccgcag cggttcatct taccttgcat
    10261 cccgcccgca gaagcccggc tacgatggag gcacggcaca gcccgcacac gacacggcgg
    10321 ccacataact gaaaacccac aaggccatgc cccgcatggc cgggtaaaag cggagccggc
    10381 ggcgacgtcc ttattccggc gagagcaggg agcacaccgc atgatctcca gaccacccct
    10441 ggaacgaaac gatcatgacc ggcatgtccc tgcgtaaaat atacatgcct ggttcaccgg
    10501 agaatatctg tgatatccac aagttttctg tcgcgcagcc agtcctgcgc ttcacgcacc
    10561 acacaactgt ccggtttcac cgctttcagc gatgatgcca gtcgcttgat caacatcacc
    10621 agttcccgtg tctgtatgtc cggcattgtc tccggcggag cagggggact ctcatttccg
    10681 acgctcatcc catgcgcttc cagtgtggca cggacaaacc aggcggcatc gaccagttgc
    10741 tgtccaatcc aggccagatc ggcatttctg tcacgggctg ccattcgctc gcccgacagc
    10801 gccttgctga tatggctgcg caccagatta tccaggagtg cctgcgcttc ttccggtgaa
    10861 aagccacctg gtatctctgt gcgcaacagc cctttcctgt acagaagctg cagcagatca
    10921 acctgcgacc gcacagcaac tatcagtaca taacggacaa cattcgcact ggcagtggtt
    10981 tctgtatcat tcagcagttc ttccggatcg ttccccccgg tgttgcagag caacggcaac
    11041 agaggtcgca tccacgcggg aacctggtat ttttcgtctg atttcatcat attacgttct
    11101 ccagcttgtt cgggatcatc cactgctcaa gtgcacgtac ctgtaccggt gcaccacgac
    11161 cggcctcacc ggcttcaaaa atcagccgcc agagtggttc ctcctgccag aaaaaccggt
    11221 caaatgatga cagtgatgga aaacgcccgc accaccccca gaaaatcgtt tccagatcat
    11281 gctcctcaac aacaccagac cagcgggaca accagcttcc gaccgtggcg ggagtatgaa
    11341 acgcaaggtc aatctgcgcc tgagcaaaac atgtctggta ttccagttcc cgtcgttctg
    11401 cctcacgttc ccgctggcgg ctgtatttct gctctgtcag cgtggttttc tgacgacgac
    11461 gctcaccttc acgaaaaggc acggacggga acagacggtg ccccgcatcg gatggcaggg
    11521 gaagaacaca gacttcccca aaactttcaa tcagtttatc cacggcagcc agccacagga
    11581 gacggttgcc atgaaattcc tccttcgtca gcgcaggggc aaaaaagcgg agatcggtca
    11641 gcgaaatccg gcgactgcca ttcaggcagc gaaagaaagc ggtggcataa ggccatgctg
    11701 aaagtgtgcg tccctgacga cgacgttgtt ctgtgacagc caccgcctgc atggcggcct
    11761 gttgttctgc gctgaggggg atcagtgccc gtgaacgtgc gctcatgcct gacctccttc
    11821 tgccatcagg atatggatga tgtcgttaac actgtatccc tgcgcatcca gtgtcgcggc
    11881 aagcgccagc gtgtgactga tcgcgacagg cgtgaagata tgttgcacct gaacgacggc
    11941 gaaaggctgt ccaccaccat tcaccagaac cagcatccag agctgagaga cgtctcccgg
    12001 cgaacataat gccaggtgga aacgttcgtg ggctggcgtg aaacgaacct ggacggggaa
    12061 aaagcccccg aactcactgc catactctcc gttcatcacc cagttagccg gtgcgtccac
    12121 ttcacgcatc acctcgtgag tgagattacg gcgcatatcg cttcccgcag cacggatatc
    12181 ttcatattcc tgtgcgttca tggactgcag ggcattcagg gtcagttcag ttttcatcac
    12241 ggtatctccc ttgctgccgc cccgtctccg gcggggcggt gtggttgttc atcaggcggc
    12301 atcagccagg ttagcggtgt gttcagtggc atccgtctgt ggacgtggtg cacacatcca
    12361 gccaggcacc cagcggttgt ctttcatatg gaactctgca tgttcagccg catcgccttt
    12421 cttcattttc tccgcgtccc gtgcggcacc ggacagtccg gcctcattca gggctgcgat
    12481 aatctgcggc tttttcaggt gtccgaagaa gtttgctttt gtcggctgcc accagtcgcg
    12541 catgtgaaag ccgatggcgg tttccagcga gtccagcgga ctgcgtgacg tgtgaccaca
    12601 ctcgcgggtc tggacaccgt taagactgca cgcggtacag aaactgagca gggataacag
    12661 cacctcctgg ctaagtgaca ggaacgtcgt catatcccgt tcccatcctt ccggcagcag
    12721 ggcagcaaaa cgagagtgtt ctgccatcag cgccatgaat gcggcacctt ccttccctga
    12781 cggtgcatcg ctggtcagcg cataatgctc acattccagg cgaattctgg cgggactact
    12841 atgcgctttg ctgtcaaaca cattcaggca gagcgtccat gccagcagtg ccagggattt
    12901 gtccggttgc tgcatcagtg ctgcctggac tgccagcgtg cgctctgagg tcaatttagt
    12961 cagcaacggc agactaattt cctccactga cgcgctctcc agcccctgtt ccgtgcggtc
    13021 agcgtcgtca gccacatcat cctcactgcg caactgcaca ccacgctgga tacacacatt
    13081 accgtaacgc caggacacca ccacgccact tccggcacgc atctccggcg tccacgccct
    13141 gaccttcgcc atgcagtcaa tcagcttcat ttctgctgcc agcaggtcag attcctcaca
    13201 ctggttttcc agcgagtcgt aacgagccat cagttcgttc agacgttcgt cttccgcctc
    13261 cgtcagcacc gcttccggct ccggcaggtt gcggtatgcc cgggcatctt cacggcactc
    13321 accgacaggc tccatgcgtc cggcgcacca ttcccagcct tcggcttccc gaaggaactc
    13381 tgcgacagcc tggagttttt ccagcagggc ggcatcgagc gccacgcggt cgacataacc
    13441 gtccccgtca tcgctgaaca aatcggtgcg cagttcgtct ggcgagaagg catcagcccc
    13501 cacgaagcgg aatttactgt tccccgccac cgccacttca ctttcggtca ccagacgacg
    13561 aatggtctgt acttccggtt taccgcccca tcccgactgg caggcggctt caaacacctg
    13621 cacctgacgc gcggtgtcgt tctccagcgc cagcgcctga cagtgctctg tggtgatgcg
    13681 gtcttctgcc agcgcatcga ggataaccgg agcaaggtct gccagtttca gcattcgctg
    13741 aacgtggcgg ggtgaatagc ccagcaaatc accgatttgt gcaggcgttt tgccttcctg
    13801 cgccattgcg cggaatccgg caatctgttc ggcagggtgc atatcccgac gctgaccgtt
    13861 ctcggtcatc gatgcggcag tcgccagctc ctgcggaatg accttcacgc gtacaggcca
    13921 gtcagccgga aggatgttgc gctctgccag catgttgagt gctgccagtc ggcgaccgcc
    13981 tgcggcgaca gcgtaacggt caccaggcag ggtatgcacg accagattct gcagcagtcc
    14041 gacgccctta atggactccg ccagttcgct gacggactcc gcagaatacg gcaccgtgcg
    14101 cacattcagc ggtgatttaa tcagcgaggc cagcggcacg ctcacttcct ccgtctgcgc
    14161 cagcagggca gacaggacag tttcctgcgt ttttacagat ttacggcttg ctttgcgggt
    14221 ggtttctggt tcacacttcg ttacaggcat aatcatcccc ttataattgc ttatgtcctg
    14281 ccggacgttc gcgcgtccgg cacctctctt acttaagaat cccgaaatcc ctgatgtact
    14341 ggttcatcca gtacccggca cagtcctcaa aattccagga gcgccagatc aacgtgccat
    14401 cctgacggac aaccaggcga aaatgcgttc cctgatcatc ctcgataaag acgttccggt
    14461 actgtgcggc aacggcttcc gcctgttcgc gggtaaagga gccgtccggc agtccctgaa
    14521 gtattctgaa atattcgctc atcctcatat ctccggtgat gccccgcaca gggcggggcg
    14581 gttgtcacag tcagcccgat cagaacggga tgtcgtctga aaacccgtaa tcctccccct
    14641 ccggcggttg cggctgaggc tctggctgcg cggccttacg tccacggcct ttcgttttcg
    14701 cgccaccttt tttcgtcgcg tcagcactct gtggctgccc attctgctga ggcttcggtt
    14761 gcgcctgagc gttctgctgt ggtgcacgtc ccagcatctg cacggtgccc gtggtcttaa
    14821 caagaatttc agtgacgtaa cgggtgatac cgttatcttc ccagctacgg gtgcgaagct
    14881 gaccttcgat gtagacctgc gcacccttgc gcagatattc acctgccact tccgcgagct
    14941 tgccgaacag caccacgcgg tgccattccg tctgctcccg catctccccc gtctgtttgt
    15001 cacgccagct ttctgatgtg gccacctgca ggtttgccac tgcgcccccg ttggggatgt
    15061 aacggacttc cggatcattg cccagacgcc cgacgaggat gaccttgttg ataccacgtg
    15121 cactcataag aacctcctga ctgaaagatg tgagataaac cggataccgg cgacgggacg
    15181 ttcatgtgcc ccttcagagg agcgaaagct cctcctgccg tccacttcac aggggtttct
    15241 ccccgcagtg gacctttccg atgggggccc gtttaccgcc tcacggggca gctgtccggg
    15301 ggcggacgca aaattttttg ccgggttcat ggctaaaaat tttgtggccg gtgcatctca
    15361 ccctggacag atggaacggg agggggttac gtctgccccc cggaaagtcc acagcgggga
    15421 aacaggccgc agggggggcg gcaggaggga ggactgctgc gccatcaggc gcagtttcct
    15481 cacacaccgt caggtgtgca gggagcgcag gtcacggcgc gacaggggcg gcgcgaccgg
    15541 aacggtcgct gcaagcccgg gttttcacgc atcggaccgt agcggcagcc cgaaaccgcg
    15601 ccagcgggtt cggcggagac tggcgggtgc gccagcagct gcccgtcgga gtcgggcctg
    15661 acgaccggat gccgccaggc agccgggagc cgaagtgggc gcaataaaag acagccagaa
    15721 acggtgacag tgtcacggaa tatcacacct gttccctgca tacagccata cactaccttc
    15781 tgaccggaaa gccgggccgg cagcgccgca ggcgctgcac cccgcgtttc cggcaggcgt
    15841 cagcctgcca ggggttttcg cgcgggctga caggcggcct ccaccgcctg cccctgtatc
    15901 acctgtttct gctgccctga aggatgtagt gaccgcccag gaaaaccgcc tgctgattcg
    15961 ccacaaaatc gcagatttca tcaacaatct gcgcggtggt gagcggtttc tgccccattt
    16021 cacgacgccg cgcattaatc acggccctgt aattacgcag aatttcgcgg gtttctgtgc
    16081 tgatattcat gacatcgtct ccccgtccag cgcctgcaac acaacgtcca ttgcttcgag
    16141 ttcctccctg acgaatgcgt caccgtcgcc cccgttctca ctcccgacgg catacaggcg
    16201 cttcatttca ctacctgcca gcgccatcac tttttccagc atcaccgccg ggactgtcac
    16261 atgacggctg gcgcgctttt cttccatcag ttcattgaca atatcggaaa caactctgtc
    16321 acatgcgcca tatacaaaag catcgtccag ccgctgcatg ac
//
LOCUS       DAZXUL010000095        16166 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZXUL010000095 DAZXUL010000000
VERSION     DAZXUL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16166
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.095"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACH3L1_003033"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACH3L1_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACH3L1_003034"
     CDS             complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACH3L1_003034"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFT4077687.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACH3L1_003035"
     CDS             complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACH3L1_003035"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFT4077688.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACH3L1_003036"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACH3L1_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="ACH3L1_003037"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="ACH3L1_003037"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFT4077689.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="ACH3L1_003038"
     CDS             complement(5877..6218)
                     /locus_tag="ACH3L1_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFT4077690.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACH3L1_003039"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACH3L1_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFT4077691.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACH3L1_003040"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACH3L1_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFT4077692.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="ACH3L1_003041"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="ACH3L1_003041"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFT4077693.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="ACH3L1_003042"
     CDS             12897..14240
                     /locus_tag="ACH3L1_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFT4077694.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="ACH3L1_003043"
     CDS             complement(14302..14508)
                     /locus_tag="ACH3L1_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFT4077695.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="ACH3L1_003044"
     CDS             14833..15387
                     /locus_tag="ACH3L1_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFT4077696.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARSAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..>16166)
                     /locus_tag="ACH3L1_003045"
     CDS             complement(15450..>16166)
                     /locus_tag="ACH3L1_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077697.1"
                     /translation="NETVNAMDNFLISFSLKDNGAEYTITLRGSGFEYEEIPITINEY
                     NSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVV
                     SKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGS
                     SVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSTIE
                     AFKKEQLALPVLVRCKNSET"
BASE COUNT         3852 a         4416 c         3957 g         3941 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcagtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatt
//
LOCUS       DAZXUL010000096        16096 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZXUL010000096 DAZXUL010000000
VERSION     DAZXUL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.096"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..588
                     /locus_tag="ACH3L1_003046"
     CDS             <1..588
                     /locus_tag="ACH3L1_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFT4077698.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="ACH3L1_003047"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="ACH3L1_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFT4077699.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="ACH3L1_003048"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="ACH3L1_003048"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFT4077700.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="ACH3L1_003049"
                     /pseudo
     CDS             complement(1511..1978)
                     /locus_tag="ACH3L1_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
     gene            2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACH3L1_003050"
     CDS             2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACH3L1_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFT4077701.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2701..3330
                     /gene="rplC"
                     /locus_tag="ACH3L1_003051"
     CDS             2701..3330
                     /gene="rplC"
                     /locus_tag="ACH3L1_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFT4077702.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3341..3946
                     /gene="rplD"
                     /locus_tag="ACH3L1_003052"
     CDS             3341..3946
                     /gene="rplD"
                     /locus_tag="ACH3L1_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFT4077703.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3943..4245
                     /gene="rplW"
                     /locus_tag="ACH3L1_003053"
     CDS             3943..4245
                     /gene="rplW"
                     /locus_tag="ACH3L1_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFT4077704.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4263..5084
                     /gene="rplB"
                     /locus_tag="ACH3L1_003054"
     CDS             4263..5084
                     /gene="rplB"
                     /locus_tag="ACH3L1_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFT4077705.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5101..5379
                     /gene="rpsS"
                     /locus_tag="ACH3L1_003055"
     CDS             5101..5379
                     /gene="rpsS"
                     /locus_tag="ACH3L1_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFT4077706.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5394..5726
                     /gene="rplV"
                     /locus_tag="ACH3L1_003056"
     CDS             5394..5726
                     /gene="rplV"
                     /locus_tag="ACH3L1_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFT4077707.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5744..6445
                     /gene="rpsC"
                     /locus_tag="ACH3L1_003057"
     CDS             5744..6445
                     /gene="rpsC"
                     /locus_tag="ACH3L1_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFT4077708.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6458..6868
                     /gene="rplP"
                     /locus_tag="ACH3L1_003058"
     CDS             6458..6868
                     /gene="rplP"
                     /locus_tag="ACH3L1_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFT4077709.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6868..7059
                     /gene="rpmC"
                     /locus_tag="ACH3L1_003059"
     CDS             6868..7059
                     /gene="rpmC"
                     /locus_tag="ACH3L1_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFT4077710.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACH3L1_003060"
     CDS             7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACH3L1_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFT4077711.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7478..7849
                     /gene="rplN"
                     /locus_tag="ACH3L1_003061"
     CDS             7478..7849
                     /gene="rplN"
                     /locus_tag="ACH3L1_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFT4077712.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7860..8174
                     /gene="rplX"
                     /locus_tag="ACH3L1_003062"
     CDS             7860..8174
                     /gene="rplX"
                     /locus_tag="ACH3L1_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFT4077713.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8189..8728
                     /gene="rplE"
                     /locus_tag="ACH3L1_003063"
     CDS             8189..8728
                     /gene="rplE"
                     /locus_tag="ACH3L1_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFT4077714.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8743..9048
                     /gene="rpsN"
                     /locus_tag="ACH3L1_003064"
     CDS             8743..9048
                     /gene="rpsN"
                     /locus_tag="ACH3L1_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFT4077715.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9082..9474
                     /gene="rpsH"
                     /locus_tag="ACH3L1_003065"
     CDS             9082..9474
                     /gene="rpsH"
                     /locus_tag="ACH3L1_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFT4077716.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9487..10020
                     /gene="rplF"
                     /locus_tag="ACH3L1_003066"
     CDS             9487..10020
                     /gene="rplF"
                     /locus_tag="ACH3L1_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFT4077717.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10030..10383
                     /gene="rplR"
                     /locus_tag="ACH3L1_003067"
     CDS             10030..10383
                     /gene="rplR"
                     /locus_tag="ACH3L1_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFT4077718.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10398..10901
                     /gene="rpsE"
                     /locus_tag="ACH3L1_003068"
     CDS             10398..10901
                     /gene="rpsE"
                     /locus_tag="ACH3L1_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFT4077719.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10905..11084
                     /gene="rpmD"
                     /locus_tag="ACH3L1_003069"
     CDS             10905..11084
                     /gene="rpmD"
                     /locus_tag="ACH3L1_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFT4077720.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11088..11522
                     /gene="rplO"
                     /locus_tag="ACH3L1_003070"
     CDS             11088..11522
                     /gene="rplO"
                     /locus_tag="ACH3L1_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFT4077721.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11530..12861
                     /gene="secY"
                     /locus_tag="ACH3L1_003071"
     CDS             11530..12861
                     /gene="secY"
                     /locus_tag="ACH3L1_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFT4077722.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACH3L1_003072"
     CDS             12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACH3L1_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFT4077723.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13156..13512
                     /gene="rpsM"
                     /locus_tag="ACH3L1_003073"
     CDS             13156..13512
                     /gene="rpsM"
                     /locus_tag="ACH3L1_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFT4077724.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13529..13918
                     /gene="rpsK"
                     /locus_tag="ACH3L1_003074"
     CDS             13529..13918
                     /gene="rpsK"
                     /locus_tag="ACH3L1_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFT4077725.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13952..14572
                     /gene="rpsD"
                     /locus_tag="ACH3L1_003075"
     CDS             13952..14572
                     /gene="rpsD"
                     /locus_tag="ACH3L1_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFT4077726.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14598..15587
                     /gene="rpoA"
                     /locus_tag="ACH3L1_003076"
     CDS             14598..15587
                     /gene="rpoA"
                     /locus_tag="ACH3L1_003076"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFT4077727.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15628..16011
                     /gene="rplQ"
                     /locus_tag="ACH3L1_003077"
     CDS             15628..16011
                     /gene="rplQ"
                     /locus_tag="ACH3L1_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFT4077728.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4141 a         3784 c         4292 g         3879 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt gctattgtaa
      721 tcataaccat tctcatttac actttgtgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagccc acgcttattt tttgacatcc tgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atctgacctg cgattagaac
     1321 tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc cgacagcgtt catgactatg
     1381 ttagccgcga tatgatgatc gagatccttg ctgatgaaga aggtcatatc gactggctcg
     1441 aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa ttacctgcaa tcacaaatta
     1501 aagttaaaga ttaacggcct atcagcaaac tatcccagcc tataacgaaa cccgcagccg
     1561 ccagaaatgg cccaaaaggc agcgggtttt ttaatgattc ttttcctctc atgagcagac
     1621 caataactac ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt ggtaagaagg
     1681 cccatgaatg ctaggcgcca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgtcg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaacaa
     1801 ttgcccccca taacgcatcg gctaaaccat caggatgaca aacttgatag aacaaaagac
     1861 ctgaccagag taacgggcac gtaaatctgt caggtaacaa gccatgcttt gcatcccaga
     1921 aaaaaagtag ggctgataaa caagcgtaca aaattagaaa gggaagggta gtgtccataa
     1981 aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagag
     2041 agcgctgatt tgttaacctg attccggtga aaaacgctat cttcacaacc ttacaaattt
     2101 cgtggaatgc agctcgcaat ctgcgcgttg cgattaattt ctacccgatg cttgcgtcag
     2161 gctcagattt gcgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctgaacc
     2221 tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg
     2281 ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa
     2341 ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca
     2401 tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca
     2461 ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc
     2521 gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga
     2581 catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg
     2641 tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca
     2701 atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt
     2761 tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg
     2821 gctaacgatg gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc taaccgtgtg
     2881 accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg
     2941 gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg
     3001 tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt
     3061 accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac
     3121 cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa
     3181 atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt
     3241 gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac
     3301 ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga
     3361 cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct
     3421 ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa
     3481 gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag gcaccggccg
     3541 tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc
     3601 tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag
     3661 catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga
     3721 agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct
     3781 gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcagaaggt
     3841 tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt
     3901 aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt
     3961 ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa
     4021 tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct
     4081 gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa
     4141 gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc
     4201 ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata
     4261 caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta gttaaagtgg
     4321 ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat
     4381 ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc
     4441 aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac
     4501 gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac aaagacggtg
     4561 aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg
     4621 ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta
     4681 ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta
     4741 cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg
     4801 aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc
     4861 atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg
     4921 ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact
     4981 ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca
     5041 acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc
     5101 atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag
     5161 aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc
     5221 tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta
     5281 tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat
     5341 cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa
     5401 ctattgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc
     5461 tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag
     5521 cggctgtaca ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg
     5581 gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga
     5641 agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc accagccaca
     5701 tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca
     5761 tcctaatggt attcgcttgg gtattgtaaa accatggaac tctacttggt ttgcgaacac
     5821 caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga
     5881 actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt
     5941 aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag acgtagaaaa
     6001 actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt
     6061 tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg
     6121 tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc
     6181 taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg cacgtaccga
     6241 atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac
     6301 ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga
     6361 gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa
     6421 gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa
     6481 attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt
     6541 cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc
     6601 agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt
     6661 cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa
     6721 cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt
     6781 tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac
     6841 cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcatt
     6901 gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag
     6961 gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca
     7021 cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc
     7081 tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt
     7141 ttgtgaaaca cccgatctac ggaaaattca tcaagcgtac gaccaaactg cacgtacatg
     7201 acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt
     7261 ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta
     7321 cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc
     7381 cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc
     7441 gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac agactatgct
     7501 gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc
     7561 gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc
     7621 gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg
     7681 tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa
     7741 caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg
     7801 tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca
     7861 tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta
     7921 aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattattgaa ggtatcaacc
     7981 tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa
     8041 aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg
     8101 accgtgtagg ctttagattc gaagacggca aaaaagtccg tttcttcaag tctaacagcg
     8161 aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga
     8221 agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt
     8281 cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga
     8341 taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg
     8401 caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct
     8461 gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat
     8521 ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg
     8581 tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg
     8641 tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc
     8701 tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg
     8761 aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa
     8821 ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc
     8881 aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa
     8941 acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa
     9001 gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt
     9061 gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt
     9121 atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa
     9181 gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc
     9241 gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa
     9301 agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga tgagctgccg
     9361 aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat
     9421 cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaatcggag
     9481 gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt tgacgtaaaa
     9541 atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg tactctcaac
     9601 gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg tgatggttac
     9661 gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat ggttatcggt
     9721 gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg tgcagcggtt
     9781 aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca tcagctgcct
     9841 gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg cgctgataag
     9901 caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga gccttacaaa
     9961 ggcaaggggg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa gaagaagtaa
    10021 ggtaacacta tggataagaa atctgctcgt atccgtcgtg cgacccgcgc acgccgcaag
    10081 ctccaggagc tgggcgcaac tcgcctggtg gtacatcgta ccccgcgtca catttacgca
    10141 caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt agaaaaagct
    10201 atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt gggtaaagct
    10261 gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg ttccgggttc
    10321 caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg ccttcagttc
    10381 taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg caggaaaagc
    10441 tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc tccttcacag
    10501 ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt aaagcgcgtg
    10561 aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg attaacgtcg
    10621 cgctgaataa cggcactcta caacaccctg ttaaaggtgt tcacacgggt tctcgcgtat
    10681 tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg cgcgccgttc
    10741 tggaagtcgc tggggttcat aacgttctgg ctaaagcata tggttccacc aacccgatca
    10801 acgtggttcg tgcaactatt gatggcctgg aaaatatgaa ttctccagaa atggtcgctg
    10861 ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca aagactatta
    10921 aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca acgctgcttg
    10981 gcttgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct gctattcgcg
    11041 gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg cgtttaaata
    11101 ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt ggtatcggtt
    11161 ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaagtctcgt tctggcggtg
    11221 gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg ccgaaattcg
    11281 gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctatctgac ctggctaaag
    11341 tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc ggtatccaga
    11401 tcgagttcgc gaaagtgatc ctggctggcg aagtaacgac tccggtaact gttcgtggcc
    11461 tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa atcgaggaat
    11521 aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg tggcttaggc
    11581 gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg tattggctct
    11641 tttattccga tccctggtat tgatgccgct gtacttgcca aactgcttga gcaacagcga
    11701 ggcaccatca ttgagatgtt taacatgttc tctggtggtg ctctcagccg tgcttctatc
    11761 tttgctctgg ggatcatgcc gtatatttcg gcgtcgatca ttatccagct gctgacggtg
    11821 gttcacccaa cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg taagatcagc
    11881 cagtacaccc gttacggtac tctggtgctg gcaatattcc agtcgatcgg tattgctacc
    11941 ggtctgccga atatgcctgg tatgcaaggc ctggtgatta acccgggctt tgcattctac
    12001 ttcaccgctg ttgtaagtct ggtcacagga acgatgttcc tgatgtggtt gggcgaacag
    12061 atcactgaac gaggtatcgg caacggtatt tcaatcatta tcttcgccgg tattgtcgcg
    12121 ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga cctgcacttc
    12181 ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt tgtgtttgtt
    12241 gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg tcgtcgtgtc
    12301 tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt aatcccggca
    12361 atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt cgggggcggt
    12421 actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca accgctttat
    12481 gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc gttggttttc
    12541 aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc aggaattcgt
    12601 ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac cttggttggt
    12661 gcgctgtata ttacctttat ctgcctgatc ccggagttca tgcgtgatgc aatgaaagta
    12721 ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat ggactttatg
    12781 gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa gaaggcgaac
    12841 ctgaaaggct acggccgata attggtcgcc cgagaagtta cggagagtaa aaatgaaagt
    12901 tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg atggtgtcat
    12961 ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat tttttcgcat
    13021 atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt ttgtatgtct
    13081 gtacgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt acatattaaa
    13141 tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca taagcatgcc
    13201 gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc catcctggct
    13261 gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca aatcgacacg
    13321 ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga aatcagcatg
    13381 agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg tcgtggtctc
    13441 ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc gcgcaaaccg
    13501 atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg cacgtaaacg
    13561 tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca acaacaccat
    13621 cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg gtggttccgg
    13681 tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag agcgttgcgc
    13741 tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag gtccgggtcc
    13801 aggccgcgaa tctactattc gtgctctgaa cgccgcaggt ttccgcatca ctaacattac
    13861 tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc gcgtataacg
    13921 cctcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc ctaagctcaa
    13981 gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg cgatcgatac
    14041 caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc gtctgtctga
    14101 ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg tgctggagcg
    14161 tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg gtgaaaacct
    14221 gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct tcggtgccac
    14281 tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg gtcgtgttgt
    14341 taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg agaaagcgaa
    14401 gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa agccaacttg
    14461 gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg agcgttctga
    14521 tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt aaagcttagt
    14581 accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg
    14641 atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct
    14701 ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg
    14761 cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc
    14821 aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca
    14881 aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata
    14941 tcacccacga cggtgatgtt gaaatcgtca agccgcagca cgtgatctgc cacctgaccg
    15001 atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg
    15061 cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg
    15121 catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc
    15181 gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag
    15241 aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac
    15301 gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc
    15361 gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta
    15421 tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc
    15481 ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg
    15541 gcatgcgctt ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatcacaggt
    15601 taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg tcaactgaac
    15661 cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact ggttcgtcat
    15721 gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt tgagccgctg
    15781 attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc ccgtactcgt
    15841 gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc gagccgtgcc
    15901 ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc gccgatggct
    15961 tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta atctgaagca
    16021 atgtaaaaaa acccgccccg gcgggttttt ttatacccgt agtatcccca cttatctaca
    16081 atagctgtac tctttt
//
LOCUS       DAZXUL010000097        16032 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZXUL010000097 DAZXUL010000000
VERSION     DAZXUL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16032
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.097"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            95..2248
                     /locus_tag="ACH3L1_003078"
     CDS             95..2248
                     /locus_tag="ACH3L1_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFT4077729.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="ACH3L1_003079"
     CDS             2301..4031
                     /locus_tag="ACH3L1_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFT4077730.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="ACH3L1_003080"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="ACH3L1_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFT4077731.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="ACH3L1_003081"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="ACH3L1_003081"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFT4077732.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="ACH3L1_003082"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="ACH3L1_003082"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFT4077733.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="ACH3L1_003083"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="ACH3L1_003083"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFT4077734.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="ACH3L1_003084"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="ACH3L1_003084"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFT4077735.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="ACH3L1_003085"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="ACH3L1_003085"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFT4077736.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="ACH3L1_003086"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="ACH3L1_003086"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFT4077737.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="ACH3L1_003087"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="ACH3L1_003087"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFT4077738.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="ACH3L1_003088"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="ACH3L1_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFT4077739.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="ACH3L1_003089"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="ACH3L1_003089"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFT4077740.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="ACH3L1_003090"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="ACH3L1_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFT4077741.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="ACH3L1_003091"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="ACH3L1_003091"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFT4077742.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="ACH3L1_003092"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="ACH3L1_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFT4077743.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..>16032)
                     /gene="nrdR"
                     /locus_tag="ACH3L1_003093"
     CDS             complement(15689..>16032)
                     /gene="nrdR"
                     /locus_tag="ACH3L1_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFT4077744.1"
                     /translation="RFTTFEVAELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVS
                     SDYVEMAINHIKSQLRATGEREVPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIK
                     EFGEEIARLED"
BASE COUNT         4368 a         3957 c         3861 g         3846 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg tt
//
LOCUS       DAZXUL010000098        15496 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZXUL010000098 DAZXUL010000000
VERSION     DAZXUL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15496
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.098"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            447..1505
                     /gene="citC"
                     /locus_tag="ACH3L1_003094"
     CDS             447..1505
                     /gene="citC"
                     /locus_tag="ACH3L1_003094"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFT4077745.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1520..1816
                     /gene="citD"
                     /locus_tag="ACH3L1_003095"
     CDS             1520..1816
                     /gene="citD"
                     /locus_tag="ACH3L1_003095"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFT4077746.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1813..2721
                     /gene="citE"
                     /locus_tag="ACH3L1_003096"
     CDS             1813..2721
                     /gene="citE"
                     /locus_tag="ACH3L1_003096"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFT4077747.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2732..4264
                     /gene="citF"
                     /locus_tag="ACH3L1_003097"
     CDS             2732..4264
                     /gene="citF"
                     /locus_tag="ACH3L1_003097"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFT4077748.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4268..4818
                     /gene="citX"
                     /locus_tag="ACH3L1_003098"
                     /pseudo
     CDS             4268..4818
                     /gene="citX"
                     /locus_tag="ACH3L1_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="ACH3L1_003099"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="ACH3L1_003099"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFT4077749.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="ACH3L1_003100"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="ACH3L1_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="ACH3L1_003101"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="ACH3L1_003101"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFT4077750.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="ACH3L1_003102"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="ACH3L1_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFT4077751.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8881..8970
                     /gene="yldA"
                     /locus_tag="ACH3L1_003103"
     CDS             8881..8970
                     /gene="yldA"
                     /locus_tag="ACH3L1_003103"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFT4077752.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8974..10212)
                     /locus_tag="ACH3L1_003104"
     CDS             complement(8974..10212)
                     /locus_tag="ACH3L1_003104"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFT4077753.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10433..10861
                     /gene="uspG"
                     /locus_tag="ACH3L1_003105"
     CDS             10433..10861
                     /gene="uspG"
                     /locus_tag="ACH3L1_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFT4077754.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10982..12547)
                     /gene="ahpF"
                     /locus_tag="ACH3L1_003106"
     CDS             complement(10982..12547)
                     /gene="ahpF"
                     /locus_tag="ACH3L1_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFT4077755.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12792..13355)
                     /gene="ahpC"
                     /locus_tag="ACH3L1_003107"
     CDS             complement(12792..13355)
                     /gene="ahpC"
                     /locus_tag="ACH3L1_003107"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFT4077756.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13727..14473
                     /gene="dsbG"
                     /locus_tag="ACH3L1_003108"
     CDS             13727..14473
                     /gene="dsbG"
                     /locus_tag="ACH3L1_003108"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFT4077757.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14683..>15496
                     /locus_tag="ACH3L1_003109"
     CDS             14683..>15496
                     /locus_tag="ACH3L1_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFT4077758.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLP"
BASE COUNT         3830 a         4021 c         3785 g         3860 t
ORIGIN      
        1 agatacgcag cggaaatgcc agtctttgga aaaatgcaaa ctgtttattc tcgttaagct
       61 gcaacattgt tttccttgcg ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt
      121 gaatcaaaat acatagatat taattatgat cgtggcaaat tttattcatt aaatcaatta
      181 aatcacttaa acctgcgagt catttcgtaa ttacattaat taaaaaactt aaaaccatta
      241 aataaataaa aaaccactaa ctctatgtga aataaatcaa aatttcacgc cgaaatactc
      301 cttaggatgt atagcaaaag gagaaaaaga tatacctcga tcacccccct ttctcccaag
      361 tgaaaataaa aggttatcag tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa
      421 catgcacctt caggatacta tttattatgt tcggcaatga tattttcacc cgcgtaaaac
      481 gttcagaaaa taaaaaaatg gcggaaatcg cccaattcct gcatgaaaat gatttgagcg
      541 ttgacaccac agtcgaagta tttattaccg taacccgcga tgaaaagctt atcgcgtgcg
      601 gtggaattgc cggaaatatt attaaatgcg ttgctatcag tgaatccgtt cgcggtgaag
      661 gactggcgct gacattagcc actgaactga taaacctcgc ctatgagcgg cacagcacgc
      721 atctgtttat ttataccaaa accgaatacg aggcgctgtt ccgccagtgc ggtttttcca
      781 cgctgacctg cgtacccggc gtgatggtgc tgatggaaaa cagcgccacg cgactgaaac
      841 gctatgccga atcgctgaaa aaatttcgtc atccagggaa caagattggc tgcattgtga
      901 tgaacgccaa tccctttacg aatggtcacc gttatctgat tcaacaagct gcggcacagt
      961 gcgactggtt gcatctgttt ttagtcaaag aagattcttc acgcttcccc tatgaagacc
     1021 ggctggatct ggtgttaaaa ggcaccgccg atattccacg cctgactgtg catcgcggct
     1081 cagaatacat catctcccgc gctacgttcc cttgctactt cattaaagaa cagagcgtca
     1141 ttaaccattg ttacaccgaa attgatctga agattttccg tcagtacctc gctcccgcgc
     1201 ttggtgtaac tcaccgcttt gtcggtactg aacccttttg tcgcgttacc gcccagtaca
     1261 accaggatat gcgctactgg ctggaaacgc cgactatctc cgcagcgccc atcgaactgg
     1321 ttgaaattga gcggctgcgt taccaggaga tgccgatatc cgcttcccgg gtacgtcaac
     1381 tgctggcgaa aaacgatctc acggctatcg cgccgctggt ccctgcagtc acgctgcatt
     1441 atttgcagaa cctgcttgag cactcccgcc aggacgcggc agctcgtcaa aagacccccg
     1501 catgagaaac aggtgaaaaa tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc
     1561 tggggatgtg atgatacgca tcgccccact cgatacgcag gatatcgacc tgcaaatcaa
     1621 tagcagcgtt gagaaacagt ttggcgatgc aattcgcacc accattctgg acgttctcgc
     1681 ccgctacaac gtgcgcggcg tacagctgaa tgtcgatgac aaaggcgcac tggactgcat
     1741 tttacatgca cgactggaag ccctgctggc acgcgccagc ggtatcccgg ctctgccatg
     1801 ggaggattgc caatgatttc cgcttcgctg caacaacgta aaactcgcac ccgccgcagc
     1861 atgttgtttg tgcctggtgc caatgccgcg atggtcagca actccttcat ctacccggct
     1921 gatgccctga tgtttgacct cgaagactcc gtggcattac gtgaaaaaga caccgcccgc
     1981 cgcatggttt atcacgcgct gcaacatccg ctgtatcgcg atattgaaac cattgtgcgt
     2041 gtcaacgcgc tggattccga atggggtgtt aacgacctgg aagccgtcgt tcgcggtggt
     2101 gcggacgttg tgcgtctgcc gaaaaccgat accgctcagg atgttctgga tattgaaaaa
     2161 gagatcctgc gtatagaaaa agcctgtggt cgtgaacccg gcagcaccgg cctgctggcg
     2221 gcgattgaat ctccgctggg cattacccgc gcagtggaaa tcgctcacgc ttccgagcgt
     2281 ttgatcggta tcgccctcgg tgcagaagac tatgtgcgca acctgcgtac agaacgctcc
     2341 ccggaaggaa ctgaactgct gttcgcacgc tgttccattt tgcaggccgc gcgctctgcg
     2401 ggtattcagg cgttcgatac cgtctattcc gacgctaaca acgaagccgg attcctgcaa
     2461 gaagccgccc acatcaaaca gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag
     2521 attgatctgc tgcacaacct ctacgcaccg acccagaaag aagtggatca cgcccaccgc
     2581 gtcgtagaag ccgctgaagc cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc
     2641 aagatggtgg acggtccggt tatcgatcgc gcccgtctgg tgctctcccg tgcagaactt
     2701 tccggcatcc gcgaagaata aggcaatcaa aatgacgcag aaaattgaac aatctcaacg
     2761 acaagaacgg gtagcggcct ggaatcgtcg cactgaatgc gatcttgccg ctttccagaa
     2821 ctcaccaaag caaacctacc aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct
     2881 ggaagaagcg attcgtcgct caggtttaca ggacggcatg acggtttcct tccatcacgc
     2941 tttccgtggc ggtgacctga ccgtcaatat ggtgatggac gtcatcgcga agatgggctt
     3001 taaaaacctg accctggcgt ccagctccct gagtgattgc catgcgccgc tggtagaaca
     3061 cattcgccag ggcgtggtta cccgcattta tacctctggc ctacgcggtc cactggcgga
     3121 agagatctcc cgtggtctgc tggcggaacc ggtgcagatc cactctcacg gcggtcgtgt
     3181 gcatctggta cagagcggcg aactgaatat cgacgtggct ttcctcggcg tcccgtcctg
     3241 tgatgaattc ggtaatgcca acggctacac cggtaaagcc tgctgcggct ccctcggcta
     3301 tgcaatggtt gatgccgaca acgcaaaaca ggtcgtgatg cttaccgaag aactgctgcc
     3361 ttatccgcat aatccggcaa gcattgagca agatcaggtt gatttgatcg tcaaagttaa
     3421 tcgcgttggc gatgctgcaa aaatcggcgc tggtactacc cgtatgacca ctaacccgcg
     3481 cgaactgctt attgcccgta gcgctgcgga tgtgattgtc aactctggct acttcaaaga
     3541 aggtttctcc atgcaaaccg gcaccggcgg cgcttcgctg gcggtaaccc gtttcctgga
     3601 agacaaaatg cgtagccgcg atattcgcgc cgacttcgcc ctcggcggta ttaccgcgac
     3661 gatggttgat ctgcacgaaa aaggtctgat ccgcaaactg ctggatgtgc agagctttga
     3721 cagccatgct gcgcaatcgc tggcccgtaa ccccaatcac atcgaaatca gcgccaacca
     3781 gtacgctaac tggggttcga aaggcgcatc ggttgatcgt ctcgacgtgg tggtactgag
     3841 cgcgctggaa attgacaggc agttcaacgt taacgtgctg accggttctg acggcgtact
     3901 gcgtggcgct tccggtggtc actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt
     3961 cgcgccactg gtacgcggtc gtattccgac tctggtggat aacgtgctga cctgcatcac
     4021 cccgggctcc agtgtcgata ttctggtcac agaccacggt atcgcagtta acccggcacg
     4081 tccggaacta gcagaacgtc tgcaggaagc gggcattaaa gtggtttcca ttgagtggct
     4141 gcgcgaacgt gcgcgtctgc tgaccggtga accacagccg attgaattca ccgaccgcgt
     4201 cgttgccgtt gtgcgttacc gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga
     4261 ataagccatg cacctgcttc ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct
     4321 gctcgtcagc cgggatgaaa ggcaagcacg gcaacacgcc tggctcaagc accatcctgt
     4381 tccactggtc tcctttaccg tggttgcgcc tgggccgatt aaagacagcg aggtcacacg
     4441 ccgaattttt aatcatcgcg tgacagcctt gcgtgcctta gccacaaagc agggctggca
     4501 aattcaggag caggctgcac tggtttccgc cagcgggccg gagggcatgt tgagcattgc
     4561 cgccccggct cgcgacctca agctcgccac cattgagctt gaacatagtc atcctctcgg
     4621 gcggttatgg gatatcgatg tcctgacgcc cgaaggcgaa attctctccc gccgcgacta
     4681 ttcactgcgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tgttttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cattcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gcggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa tgacgaacgg
     9961 aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc ttcccggttt caacgacttc
    10021 ccccataaat tcatgaccaa aaatatcgcc atgtttaacc tggggtattt tgcctcgata
    10081 aagatggagg tcagagccac agatcgccgt taccgtaata cgcagaataa tatcatctgc
    10141 ctgttcaatg cccggatcgg gaacattttc tacctgaacg tgatgtgggc cgtgataagt
    10201 caatgctttc atgcaacctc cagagacagt tcaaaaaaga gtcagtcttt aagggtagct
    10261 atcacagcgg gaaaacgggc ggtaccgcag gttttgggag aattcctagt gttcagtgag
    10321 aatatattaa tcacagaaac cgttcgtaag gaaaaagaac cagacaaata tcagggataa
    10381 atattataat catggagaat gatttgttca tgattaacag ggagaaaggg ttatgtataa
    10441 ggcaatcatt atgccagttg atgtatttga aatggaattg agcgacaaag ctgttcgcca
    10501 cgctgaattc ctcgcccagg atgacggagt tattcatcta cttcacgtac tacccgggtc
    10561 agccagcctg agcctgcacc gttttgccgc tgatgtgcgt cgttttgaag agcatctgca
    10621 acatgaagca gaagaacgtc tgcaaacgat ggtcagccac ttcaccatcg atccttcccg
    10681 cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa gtcaatgagt tggcagaaga
    10741 actgggggct gatgttgtag ttatcggttc tcgcaaccca tcgatttcga cccatctgtt
    10801 aggttctaac gcctcgagcg tgatccgcca cgccaatctg cctgtactgg tcgttcgcta
    10861 acagataaaa gtatttatgc cgccctgcct ggaacatccc ggcagggctt tttaatgcca
    10921 taaaaaagcc gccaggtttg accccggcgg ctaagcaatt gcaggtgaat cttacttctt
    10981 cttatgcagt tttggtgcga atcaggtagt caaaagcact cagagaggct ttggcacctt
    11041 cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt acagtcaccc gctgcgaaca
    11101 cgcctttgac gttggtttca catttcgcat caatgataat ctcgcccatg cggttacgtt
    11161 cgactgcgcc ttccagccag ttggtgttcg gcagcagacc aatctggaca aaaataccgg
    11221 ccagttcgat gttgtgaata ttgccgctga cacgatctcg atattccaga ccaacgactt
    11281 tgctgccgtc gcctttcact tccgtggttt gcgcattcag aataatgtcg acgtttttca
    11341 ggctgcgcag tttgtcctgc agaacctggt cggctttcat ttctggcgca aattccagca
    11401 gcgttacgtg ctcaacgata cccgccaggt cgattgccgc ttccacgccg gagttaccgc
    11461 cgccgataac cgctacgcgt ttgcctttaa acagcgggcc gtcgcagtgc gggcagtagg
    11521 tcacgccttt agtgcgatac tgatcttcgc ccggaacgtt catgttgcgc cattttgcac
    11581 ccgtcgccac gataatgctg cgtgctttca gtaccgcgcc agaagctgtt tcaatctggt
    11641 gtagaccacc ttcaaccgct gccgggatca gtttgctggc gctctggctg tcgatcacat
    11701 caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc caacttctgc ccttcagtct
    11761 tcggtacaga aatgtagttt tcgatatcaa cggtatcgag gatctgacca ccaaaacgtt
    11821 cgcccatcag accggtacgg atgcctttac gtgcggagta aattgctgcc gctgcacccg
    11881 ccgggccgga accaacgatt aatacgtcat aagcatcacg cttgttcagc tcttctgccg
    11941 cacgattttc cgcgccagta tcaattttgg caacgatttc agtcaacgtc atgcggccct
    12001 gaccaaactc tttcccgttt acgaacactg ccggaacgcc catcacgttg cgatcggtga
    12061 tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg ttcaatacgc
    12121 tcatcaggtt cagcgcctgc accacgtccg ggcagttatg gcaagagagc gagtaatagg
    12181 tttcgaattc aaaatcaccg tcaatatggc gaatctgctc cagcagagac tgcgcttctt
    12241 tcgacggatg accaccggtc cacagcaacg ccagtaccag cgaggtgaac tcgtggccca
    12301 gcggggagcc tgcaaaacgt ggtccctggt tggaacctgg gttggtgatc aggaaagacg
    12361 gcttacgcac tggcaagctg ttatcttctt taaaggtgac tttgtctgac agttctgcga
    12421 tttcagccaa cagttccttg atttctgccg atttagcgct gtcatccagc gtggcaatta
    12481 actcaacagg cttggtcaat ttctcaaggt aagccttgag ttgagttttc atatttgtgt
    12541 cgagcatgtt tatctcctgg gctttaaaca tcatcatgca agcggcctct gcggctacct
    12601 ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac ccgaagaatt
    12661 aggtgaattt ccgaagtatt tcgcgtcaga gcaaggcgac tagcctgtga atccccggga
    12721 gcttacacaa gtaagtgacc ggggtgagcg ggcgacgcca acgccgctat ggcgtgaaag
    12781 acgaaggaaa tttagatttt accaaccagg tccagagacg gagccagagt tgcttcacct
    12841 tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg agctgctttg
    12901 attttacgca gcagatcaga cgcgtcacgg ccaatgcctt cagcggtaac ttcgattgcc
    12961 tggatgatac cctgcgggtc aacaacgaag gtcgcacggt cagccagacc ttcatcttca
    13021 cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat cgcatatttg
    13081 attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg agtatcggta
    13141 gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtagtggtc agcaacgtca
    13201 cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa gacgctccag
    13261 cggccttcgg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa tgcctggttt
    13321 ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt gttttcgatg
    13381 agatgtaagg taaccaattt ttgccgattc ggctaatgcg tttccattat caaatcgata
    13441 agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc agcggcgata
    13501 aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct gaactaccct ttggtatctt
    13561 cagcgcctgc ctgcaggctg aggtgttgcc cttacaaatg caacaacgac atggattaca
    13621 acaccctcat aaacaaaggg caatcacctg atctaagctc ttacctatga cagtgatagg
    13681 ttatgccttt tactcgactt ttgcactgac tgaaaagaac aaattaatgt taaaaaagat
    13741 acttttactg gctctgcttc ctgcaatcgc cttcgcagag gaacttcctt ccccagtaaa
    13801 agcgattgaa aaacagggca ttacaatcat caaaacgttc gatgcccccg gaggaatgaa
    13861 aggttatctc ggaaagtatc aggatatggg cgtcaccatc tacctgactc ccgatggtaa
    13921 gcacgctatc tctggttaca tgtacaacga gaaaggtgaa aacctgagta acacacttat
    13981 cgaaaaagaa atttacgcac cagccggacg cgaaatgtgg caacggatgg aacaatccca
    14041 ctggctcctc gacggtaaaa aagatgcgcc ggtcattgtc tacgtcttct ccgatccgtt
    14101 ctgcccatat tgtaaacagt tctggcagca ggcgcgcccg tgggtagatt ctggcaaagt
    14161 gcaattaaga acattgttgg ttggggttat caagccagaa agcccggcga cagcagcggc
    14221 aattcttgcc tccaaagatc ccgcaaaaac ctggcaacaa tatgaagcct ctggttgcaa
    14281 gcttaagcta aacgtgcctg caaacgtaag tacagagcaa atgaaagtgt taagtgacaa
    14341 tgagaaactg atggacgatc tgggggcaaa tgtcacgccg gctatctatt acatgagtaa
    14401 ggaaaatacg ctgcaacagg ccgtggggtt gcccgatcag aaaacgctta atatcattat
    14461 gggaaataaa taaaaggtaa ttcatggatg gttgaactat atccctgctt ttcaaccatc
    14521 ctaaaaaagc atgaatttct cgctctaccc taagtggctg tgctatttta gaaaaactta
    14581 atcctgagtt aagcaaaaaa acataatcaa taaaaataca tacgcaaaaa acattgatta
    14641 agtgaatata ccatggaaga aaaatataaa ccggagtagt gtatggccaa tctctacgac
    14701 ttgaaaaagt tcgacttaaa tcttcttgtc atatttgagt gtatttacca gcatttaagc
    14761 attagcaaag ctgctgaatc actatatatc accccatcag ccgtgagcca gtcgttacaa
    14821 cgcttacgtg cgcaatttaa tgacccttta ttcattcgct caggaaaagg tatcgcgccg
    14881 acgacaacag ggttgaatct gcatcaccat cttgagaaaa acctcaaagg ccttgaacaa
    14941 acaattaata tagtcaataa gtccgaacta aagaaaaact ttattatcta cggccctcag
    15001 ctcatttctt gctctaataa tagtatgctg atccgctgcc tacggcaaga tgcctctgtc
    15061 gagattgagt gccatgatat cctcatgtca gcagagaatg ctgaagaact gttggttcac
    15121 cgcaaagccg atctggtgat cacccaaatg ccagtgatca gccgttccgt catttgcatg
    15181 ccattgcata caattcgcaa tactctaatt tgtagtaata agcatccgcg aattactgac
    15241 aatagcactt atgaacaaat tatggcagaa gagttcaccc aattaatagc caaatcagca
    15301 ggagttgatg atattcaaat ggaaatagac gagaagttta tgaatcgcaa aatttcattt
    15361 cgtggttcct ctttgctgac aataattaat agcattgcag ttacagattt actgggaata
    15421 gtcccctatg aactttataa ctaccaccgt gactttctta atttaaaaga gataaaacta
    15481 gaacatccgt tacctt
//
LOCUS       DAZXUL010000099        15237 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZXUL010000099 DAZXUL010000000
VERSION     DAZXUL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15237
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.099"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(241..486)
                     /locus_tag="ACH3L1_003110"
     CDS             complement(241..486)
                     /locus_tag="ACH3L1_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077759.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1263..2105)
                     /locus_tag="ACH3L1_003111"
     CDS             complement(1263..2105)
                     /locus_tag="ACH3L1_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFT4077760.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2190..2387)
                     /locus_tag="ACH3L1_003112"
     CDS             complement(2190..2387)
                     /locus_tag="ACH3L1_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFT4077761.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2407..2895)
                     /locus_tag="ACH3L1_003113"
     CDS             complement(2407..2895)
                     /locus_tag="ACH3L1_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFT4077762.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2892..3269)
                     /locus_tag="ACH3L1_003114"
     CDS             complement(2892..3269)
                     /locus_tag="ACH3L1_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFT4077763.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3316..3693)
                     /gene="cbeA"
                     /locus_tag="ACH3L1_003115"
     CDS             complement(3316..3693)
                     /gene="cbeA"
                     /locus_tag="ACH3L1_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFT4077764.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3773..3994)
                     /locus_tag="ACH3L1_003116"
     CDS             complement(3773..3994)
                     /locus_tag="ACH3L1_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFT4077765.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4081..4557)
                     /gene="radC"
                     /locus_tag="ACH3L1_003117"
     CDS             complement(4081..4557)
                     /gene="radC"
                     /locus_tag="ACH3L1_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFT4077766.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4573..5046)
                     /locus_tag="ACH3L1_003118"
     CDS             complement(4573..5046)
                     /locus_tag="ACH3L1_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFT4077767.1"
                     /translation="MKTLSQNTTSSACAPETDLQQLVATLVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(5388..6206)
                     /locus_tag="ACH3L1_003119"
     CDS             complement(5388..6206)
                     /locus_tag="ACH3L1_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFT4077768.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(6385..6516)
                     /locus_tag="ACH3L1_003120"
     CDS             complement(6385..6516)
                     /locus_tag="ACH3L1_003120"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFT4077769.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            complement(6617..7072)
                     /locus_tag="ACH3L1_003121"
     CDS             complement(6617..7072)
                     /locus_tag="ACH3L1_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFT4077770.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(7148..9664)
                     /locus_tag="ACH3L1_003122"
     CDS             complement(7148..9664)
                     /locus_tag="ACH3L1_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077771.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTNPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            complement(9785..12907)
                     /gene="agn43"
                     /locus_tag="ACH3L1_003123"
                     /gene_synonym="flu"
     CDS             complement(9785..12907)
                     /gene="agn43"
                     /locus_tag="ACH3L1_003123"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFT4077772.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            complement(13241..14113)
                     /locus_tag="ACH3L1_003124"
     CDS             complement(13241..14113)
                     /locus_tag="ACH3L1_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFT4077773.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            complement(14223..>15237)
                     /locus_tag="ACH3L1_003125"
     CDS             complement(14223..>15237)
                     /locus_tag="ACH3L1_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFT4077774.1"
                     /translation="NEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIRNV
                     NLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDELAT
                     FFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNPSS
                     HNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMCQW
                     FWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRINH
                     AWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLEHK
                     RRK"
BASE COUNT         3445 a         3976 c         3969 g         3847 t
ORIGIN      
        1 aagtatgtca ggcttttttc cgtaatgctc taacttccac gcattggttt ctgacatcaa
       61 ataaggctat gactttctct cccttttaat ctcattctga tctaaaacca tatcttaaaa
      121 aaatgcatgg gtattttaat tattttgatg ttgatctaac gcactcaagg accattttat
      181 taccgattat ccattctatt taactgttgc gatataaatg tttgttttag gtgctttaat
      241 tcagtacaag tcatcaaacc gtaccagacc ctgactgtca gtgacaccga caggtggggg
      301 cggcggtgtg gggtcaaact gagtgtgtct gcctctggcc cacagagcca gctctgccgc
      361 cattgcccgt ggatgatact ccagctgcac gttgagaccg gaaactgggt tgttgttctc
      421 atccacgaac tgcagcgcca gccagtgcat ccgctgctta ccgcaggcga cttctttgta
      481 cttcattttg tgactcaggc aggtgactgt cgtgttgcct gttgcgtcca ttttgcggtt
      541 aaaccctttt aattcaattt gttaaattgc tttccacctt ttgcggggcg cgacttaacc
      601 aaaatattca ctgttatcat aatatgcaat gggctggagg ttgcaggtca tttagggatt
      661 ttctgtaagc cccgttttgc agaacgttct atctccgatt gagatcctgt ctctcggtat
      721 taccggtagt ctggccataa tcccctatgg atcatattga tatagtaggc ataacaccca
      781 aaagttgact aaagctaaat caatcagagt gaaaaatgtt cggtttgatg ttggggggga
      841 ctgaggaggg gccaacgcag gtggtatgcg ttttacttca gaccggacaa agaccggatg
      901 tatccgtgca gtaatgaatc cgttagccag gtgctcatgt ggaaatagtg tctattgggg
      961 atttgaagtt tgacagaaaa ggccacagtg accagaaggg ggctacttag aagaaatgtt
     1021 caggctgggt ggaagaggta aagcagtgac atcagcattc aaagtgaccg gatatctacc
     1081 agaaccccag aaagcaaaaa accagcccgt aggctggttc ttctaaatag tggtgccgga
     1141 ctcggaatcg aaaatttctt ttatcatatt gatattaatg caatttgttt ggtttgatta
     1201 aaaatgtacc ctcaattata cccgcagtcc cttttgatat cgaaagggaa tcactctcgg
     1261 gatcaggctg agagcacaga aggaaagtac ctggctatta tatcgttaat tctgtcaatc
     1321 agttccagcc ttttgaaagt gatgtgtgca gatcccttct taaagtattt aatgatgaac
     1381 atctcgtctt catagcgctc tttaccctga acggactgga tgtgaccatc cagacgaata
     1441 gtgatgtttt cccggttgtc ggggatcggt ttgccgctga acagatgtag cattctttcc
     1501 aggtcgacga gtcggtcagt ctgttgcccg gtgataagat gaaaccccca ccggtcccac
     1561 ctcaccaggt tgttgacgat aattttactg ccaaatttac agggtgagtt ggttttgtaa
     1621 tcccagctca gccctttgaa gacgttgatc actccccgtt caaacacttc atctttgttc
     1681 tggtgcagtt gttcaaatgt gctcagaatg ttcgcttcgc tgatttccgg aaaattatca
     1741 tattccagtg acctgtacca tgtgtcccgg gtctgcgcat ccatcagact cagcatcccg
     1801 gaacgttgca tcagatcacg ccagagttcg cgatcaagat tgcgtgtgat ggctttcatt
     1861 gcggtttccg gtttctccat cagccagcaa ccgcagcgaa aatcctgttt catggcccag
     1921 tccagagccg ttttacctct aatactttct gtcattgctg atacttcagc cagttgtcgg
     1981 ataagtgtgt cgatttgcag caatgtactc tttcgtacag ccagaatgtg gcggatactg
     2041 gttgagcaga taacgtccgt atgcccgttg accacttccg gacgagaggc tgtgatttca
     2101 gacataatcg atattccttt ttgcagataa acgaaaacgc cagccgtatt caggctggcg
     2161 tcagtggaat gaagtctggt gagtaagact caccggttct gttgcagaaa ctgcagctga
     2221 cgcagggtac ggatatcttc cctggcctgt tcaatacagg gcagcagtgc tgccgaatcc
     2281 gttttatcct catcgttgtc gaagataatc cctgattcgc agtcaatatt gtcctgcagc
     2341 cacgcaatca gaatatccag cgccgtttct gtggttaata atttcatcca gtgtcttttg
     2401 tcctgattac cagtcgaaag tggggaaccc cggcagacat tctgcgccgg cgtccagatg
     2461 aatgagactg acgccataac gccgggtgag agtgacgacc agacgacgga acgttctgga
     2521 caggcccagg cgtttaagcc gcagaaccgg gtacgaccat gcatcggtac gtaacagata
     2581 gccgctgcct gtaaaatgaa tccattctgt ttcgccgtcg tcattagtct ggtgtgacag
     2641 cgagtacagc caggcgttgt ccgtttccgt gatatgtgcg gtgctgcaac gtatgcaggt
     2701 aagggttgtt aacggtggtg gtgtactggt tgcgtgttgg tcggatttat cgaccacgcg
     2761 gagtgaataa ccgttttcag agaccttgtt aatcagttca ctgagattaa caccatcgac
     2821 gtcaacgaca atgcgcccca tgttcagtgc ctgcacgtta acgctgtcgg cttccagcgt
     2881 cagggccagt ttcattgttt cgcttccgga tgcttccccc gggtaatgtt atttaccgtt
     2941 ctgtagttat cgcgggtcat caggccggtt gcccggcgtg cccggagaat atcaatgctg
     3001 ttgattaact gcgacggggc tcctgcgctg aatcccggct ggtcggtacg taccagcgcg
     3061 tatttttcaa cgagaaagtt caccgcgtca cacagtgaaa ttcctgcctc aatatgctgc
     3121 tcaatcacac gttcatcagc gaacggtgtg tcattcagcg tgaggccata gtgctggtcc
     3181 agcagtcggg tgagcagtgt ctgccagatg gtgatgggag acgggcagcc cgatgcctcc
     3241 cgtacgtgag tgtcgggtaa tgtattcatc ttaagttctc tgtaacaggt ggttataaag
     3301 gggagatatc cggtgttata cggtggttgc gggcgctgcc ggtgtcggat aaacagccag
     3361 ataaacgtaa ccacaggagc cgagggtgtc ggcttcgcag gtcagccctt tcgcatacag
     3421 tgtgacggta tgctgatggc ggggattcag ttcaccgcca gtgagcatga gttccagttg
     3481 tttcatcagc agcggaaagg cctggtccag gtggtacgca tctacgtcgc tgaacctgcc
     3541 tctgataccg gcgcggtctg caaggtaatg caggcggtta ccctcctgta ccagtcgggc
     3601 cccgaaacag ggcgtcacgg tgcagggcag cccccaccag gggcggtcgt gattgtcgtc
     3661 gggaggcgtt gtcccgggga gtgtgtctga cacgataaag tcctcacaga aaatcggtta
     3721 agaatggtcc ggtagtggcg ataattctgc tcatcagaat tcccttccag actcagttca
     3781 gggtgacgct catcagccgg acatacggac caaaactgtc cttacggcgt tctgcaaaca
     3841 cagccagtac accgggaata tcctgcactt cacggccggt atatgtatcc gtgctaccgt
     3901 gccagcggta tttaccggta cagaacggaa aaagacggga tgcaggatgc tgacggtgaa
     3961 tacgcatggc ttcaccacgg gtgatgattt tcatgagaat agtcctcctg aatgtgaaag
     4021 taagaaacag ctgagaaaga gaaactgaag tgatatgaag gtgattgtga cggtgacggg
     4081 ttaaagcaga ccgtgttccg caaaggagaa aacctggctg ccaccgacta tcagatggtc
     4141 cggtacccgg atatcaacca gggccagtgc ctgtaccaga cgctcagtga taaggcggtc
     4201 tgccttgctg ggggggactt caccggacgg gtgattgtgc gccagcacca cggcagcggc
     4261 attgtggtac agggcgcgtt taatcacttc ccggggatgg acttccgtac ggttgatggt
     4321 gccggtgaag agggtttcac cggcaatcag ctgattctgg ttgttcagat acagcacccg
     4381 gaactcttca cgctccagtc ccgccatgtt cagaatcagc cattcccgtg ccgcatgcgt
     4441 ggaggtgaag gccacgccgg gttcatgaag atggcggtcc agggttttca gggcccgcag
     4501 aatgaggctg cgctctctgg ttgtcatttc tccgggcaga aaggaaagct gctgcattgt
     4561 ccctgccacc attcagtcga tgatgcgcat aatggcgctg cattccggat gctgcagggc
     4621 gtaatcccgc agccggtaat aatggaccgt catggcataa cactccgtac gacaggcatg
     4681 atgactgtac gtcatcagac aggcagcaat gccggcagct tccggactca tttcagcgcg
     4741 gttaccgttc atggcattga acagtaccca tgtttcgtca tcatcgtcat ccggttcggg
     4801 tgccataaat gccccgccgt tgttcagggt gtacagattc cagataccac cgcagtagtc
     4861 ttcgcacaga cggtccatcc agccgaagac acggggctcc agggtcaccc actgtggaat
     4921 gaggccaaag tgctgcggcc agaagctgat gcgctgttca tcagggacga gggtggcaac
     4981 cagttgttgc aggtcagtct ccggtgcaca ggccgaagag gtggtgttct gagacaatgt
     5041 tttcatgtat gaaatccttg taatgtgaat aaatgagagg gagtaaaggt cgggagctgg
     5101 caacatcacg ttcatcactg accgtgatga cccggtaata tcagggatga atgatattcg
     5161 gctgaaattc cggacagccg tacgcgtaac cgttccggtc acagacggat ggccagatgc
     5221 cattgcctgt atactcagtc tgcacagact gatttctccg ggacggtaaa gcagacgggt
     5281 acaccaggcg cacaggtcat catcagaaat ggaacgacac cccggcagcg cctccggggc
     5341 gtcgggtgat tctggtgcct gaatcacgcc ttcagggaca tgctgcatca gcgcaggctc
     5401 tccagcagcg tttctgccat cacccacaat gcgcggttga gcttaatatc ggtgtcgatg
     5461 ttgtgaatgg cacgggtgtg gatacgtttt ccttttgcac tgcggccgga aatcccgcct
     5521 ttcagcatat tctcctggat ggtctgataa gtactccaca ggtccttacc gtaatcctcc
     5581 cggcgtcgtg gcgtcagaat gtcggcggtg gtgacgggct gatgttcgtc accataacgg
     5641 taagtcagtg ccgcctgtgc cagcgcctgg cgtgccggtg gcggcaggac cagcgactgc
     5701 atggcatcac gtttctcctc aatccggtca aacacgccca ccacttcgta agccccttca
     5761 ataactttct ccaccacgtt tccccggtgc ggaacacgca cttcccccag agactgacca
     5821 cagacacacc cgttctggca gacgaatctg aagtaacccg gcagcatctg gtagctggag
     5881 gtaccgtcat gggagttgag cagaataatt tcagggacat gttctccgtt tatctctccg
     5941 gcccgccgca gacgcagcat gtgttttgtg tatccccggc ggcccgggtc gcgcacacgg
     6001 gtctggcagg cgaaaaaggg ctggaagcct tcccgctgca gactttccag tacggtgatg
     6061 gtggggatgt acgtatagtt ctggcttcgg gacgtgtgtt tctcttcccc gaaaatcccc
     6121 ggcacatggt gcatcagctc ttcatgtgtc agcggacggt cacggcgtat ctggttcgca
     6181 taaccaaaac gactgactaa ttgcatactt tgctccttat cggtggttaa gatttactgg
     6241 tgcaacaaat gaaaaaagcc gcgtctcccg gagaagacgc ggcctgatgg atgaaatcaa
     6301 tgaagtttat tgtctgagaa gccctccact ggcgagccac gcattaagct gttttccggc
     6361 atcagcttca gagttccggc tgcgtcagcg tcgctgacct tccgcgttgc ggataacccg
     6421 acggaaatgc attcccgggt catcctggat aaacacattg cggtaagcgg ttatgacagc
     6481 aaccgcctgc ccgtatgaga aagatccttc agccaggaca tactctgtgt gtaacccggc
     6541 atatctggtt tctcctgata aatagcctct gccatacgtt gtggcagagg ctgaagcatg
     6601 aaactgactt cagggatcag ttaacatttt ttccggaaac ggtaatcagc agtggatggt
     6661 agtcctgggg atcgaaaacc gataacggca gactgacacg atggccgtta ctttcttcag
     6721 ttgctttaat gatttcggtt gtggcgacat tttccacgca ctccgtttcc agaaatgcgt
     6781 ctgtggttcg cgtggcatta ctgtcaccaa aagcttccgt ttccattttt ctggtcacca
     6841 gcgtctgacc atatttgtct ttgagttgca gagtgatggt gagggggcca aatccttcac
     6901 cgtttccgcc attatccagc cggaactggt aagcacaaat atttcccggg agccatatcg
     6961 tatctgtatt gcgtatactg atgtaacgtt gatcctgtgc ccggagtggg gcagaccacg
     7021 ttaaccccag aatgaaggcg gtaatcatgc aggttttgaa caggtgaatc atggtattta
     7081 cctctctgag tcatgacgat gacactggca aatcaggtga taaaacctaa aaggagcaga
     7141 acacacgtta tgccgggagc tccgggggga atgcttcttc cagccggtta accatcttgc
     7201 cgagatggga tgcatcatat tccatgacgg ggcgttgcct gatgatactg accaccagtg
     7261 gtttgattaa catattggtc gcggcccgtt gttgtatacc ggcggcgaaa atgatctcca
     7321 gagcattctg atcgataaca cgggccagat tcgcgttcag attttcaatc tcatgtccgg
     7381 tcagcaggaa actgccggca agccatgtca cctgctcctg aatcattgcc agaattttga
     7441 cgcgatcttc ttcaaccagc gctccggcaa tttttcctga tacagcggag gcaatcattc
     7501 cccccagaac accgccaacc acacgccctg tcagtgcacc aaatggtccc agtggactga
     7561 acaatatccc gcccgccact gacccgacag caccacctgc cacaccggaa gatgccacgg
     7621 caagattcct gatgaactgc gcaccggaga tccgtccccg caacattttg atcatgtcag
     7681 ggctggttgt gacagctacc agtgccagag atgtcaccag cgtacccttc atcactttgt
     7741 tcagggcact gatatttcct gcaccggtcc ccttctgaag cgcctgccgg acagtcgggc
     7801 tggccgtcga aaaatcaata tgcttcagca taccctgaac aacagtgagc cggtgaagtt
     7861 gctgggttgt gacgtagaca gccagtgtgc gggtgaaggt ttttcctgcc tggacagcag
     7921 ctgtctgcag ggcagcatcg cgatcgccgg tgctgagcca gaagaccgat gccgtcaggg
     7981 caaaactgat ccctccggcc gccagactga caaccgaccc ctcggcaata tcataagtga
     8041 ccgattcgat ggtcccgaac cgggtgatat tacgggcctg ggtataagtc agatgtcccc
     8101 gacgaatcag ccgggacgct tctgcgggat tggttacacc gggtacttta ccttcgcgga
     8161 tcttattctt catggtttcc acagcgccgg catactgatc acgggggact tccagttgca
     8221 tgggaccatg atttcccatg taacgatact gtccgttttg tccgtcgaat gccgcaccga
     8281 cgctacgggc tgcagttgaa caatatttcg tctggatttc tgttccgctg accagtctgt
     8341 ctgctccatt tctggcatta ttatcaccga ggatccgtgc gtttttcaga cggactctgt
     8401 cgacaatatg gtttccccgt tcagcggcaa acccatgtcc ctgccctccg gaagcaaaca
     8461 ttttctcatt tgtactccag gtttccggca gtttcatgag aatcagacca ttcgccgttg
     8521 catcggaaat aaaaccggtg taagaacgtt cataaatgtc ctgacacagt gtttctgttt
     8581 cgtgacaaag agaggagcag taccattccg gagccggctt atgattttcc ggggcatgat
     8641 gtacttctgt cagcccgcag gaggcacaga tacgaagctc atccacgtgg tggaatgata
     8701 caaaacggcg gccttctgaa aaggggtacc agtcaccgcc gtccaggcaa cgatcgagaa
     8761 aatcgtcatt cgcttccggt gataaattca ggcggttccg gaagagcgtt ttcagttcat
     8821 caggtgtgaa tgacaggttc tctttcaact gttcgttaaa ccctgccatg tttctttcat
     8881 accaggcatt gttcagtaac cagtttccgt ctgccagttc aaattccctc agttctcccc
     8941 gggaaatccg ttgtgaaatg agtgtcagcc agatgcgctc cgtcagcgtt tcaggaaggc
     9001 tgtaatcgtt ttgcaaactg ctcagccacg atttactgtt gagcaccggt tgttcttcca
     9061 gtgtctggct gatggcactg gtcagtcttt tcagcgcctg gatctggctg gccgcccata
     9121 gcagcgtcac taatgcagcc agccagtaca acaacccgcc ccaccacatc agtgagggtg
     9181 aaatatcgaa ctgcaatgcg atcaatggtg cgtatgcaga acacgccata aggaaaaaag
     9241 cagtataaaa acagcagtaa tacccgttaa tttccttgct gatatcttca ggtgtgagcc
     9301 cgttctgctg aatggttctt atctgcacgg gaagagccag cagaccagcg gcgagagcgg
     9361 tggtcagaat aaattttaca gagtcaaaag tatgagcctc cagtgcgata tgtttaccat
     9421 caagtcccca catgaagctg atgatcattc cggcaatcgt cagaaatacg cctgccagcc
     9481 agcgaatatg gaataaccgg gttatgcgtt cagtgatata gccagccagt aaagccggac
     9541 cgaacagaca cagcaatatg aaccctgcca gcgcagacgc tgtgctggtt aatgctctga
     9601 acaaaaggcg aagaatggca aatcctgcga tcagcagaat cagcgcgccg actatctgta
     9661 acatagtcac tccgtgatga atatcatgtt attgtgaatg ccagtgaatg tggcactgaa
     9721 acgttttccc ccgtccgggt cccggtcatc atgaccggga ccacagagag gcaatgcaga
     9781 attatcagaa ggttacattc agtgtggcct gactgttata accttcagcg ctgctgccgt
     9841 tgatgctgtg ggcataactg gcctgaacgc ccagggtgat attttcccgg acacgggctt
     9901 ccagtccggc ctgcaggtcc agtgacgtac cattctgtga cggtgagaac gtcatgttac
     9961 tgccggctgc ggctgtaccc atgctcatgt ccccccggga gctgaaggtg cggataacag
    10021 aaggctgtac ccaccagttc accggcagtt cacgcacact gtgtttggca ctgccgcgca
    10081 gggtgtcacg ggatgaggtg cctttaccaa agttcatatc gtggtggctg cccagacgga
    10141 agccggcacg cacatgttgt gcactgccat gcccgaactt cacataactg gcgttatcct
    10201 ggccgtcatc cagggagagt ccctgccagg tgtactgcag ttgcggctcc agcatcagat
    10261 tgtcagtgat actgaagggc agaccggttt ccagcgaacc cagccagccc cagccccgga
    10321 cgcggaagtc gttattgtcc gatgaggctt tcatgctgtg gcgggttccc tgggccacaa
    10381 tgtcagccca caggccggag gcgttgtgta tcagattcag gtatccgccc aggctgccgg
    10441 catcatcccg gaccgtgccg gcacgggagc cgtcatcatc cttaacatca acggaagaat
    10501 ggccagcagc accatatacc cccgcggtca cagacatacc ggcaacctct gttctgagca
    10561 ggtcaccctc cagacggacg aagccatagc tgccgctgct ttccggcgtg gctccacggg
    10621 caataccgcc gttgttatcg tgaccgagat gaccgccctg aatgctgaga cggacgctgt
    10681 tattttcacc gcttacaccg gtctgatggc tgcgggagcc tgccagaatc cggtcatagt
    10741 ccattgcctg tgtcagcatg gaggcataca gggggacttc tgcacgataa gcattttcac
    10801 tgcgcagata ccagctctca tcactgtccc ggttgaggga gtagttaaag gcaccggcct
    10861 gcagcttatt cccctggata aaggcccctt cctccgtggt ggcaccgtta atggcttcca
    10921 ccacctgaat acccttaccg ctggtcgcca gccccgacgc actgttgccg gcgttcacca
    10981 ggttcaggat ggtttttccg gttgccctgc caccgtcaat gaccagtctg tcagcattgt
    11041 tctgtgccat atccgggcgt acacgcaggc tgatggtgcc attctgtccg ttcaggtttt
    11101 tcactttcag ggttgccggt acgaacttcc ctgtgcgggt ggaggtgaaa tgaatctgtc
    11161 cggcatggct gaggtcatcc accaccgact gcaccgtggc gttatcggga atattccagg
    11221 tggcaccaga ggcgagagtg acattcgtgg ggtcaatggc accgttcagc acagtgctgc
    11281 cggtcagctt cagggctgta ccgcgctgag caatgacatc cgtggtgacg gtactgtcgt
    11341 tcagcgtcag cgtgccttca ttcaggttga cggctttctg ggtgagtgtg gtgttgctga
    11401 cagtgagtgt gccacttcct gatttttcca cgctgccgtt accggtgaga gcgcctccct
    11461 gcaggagtgc atcgccttca cggatggtca gggtatcgtt gttcaccgtc ttcccggaaa
    11521 gggtaagtat ggcgccgtta ttcagcgtgg tggtgcccgc cagtgtgccg cccctggcgg
    11581 tgaacagtcc gccatttacc gtggtatccg tggccgtatc accggcgttc agcgtgaatg
    11641 aactgccttt ttccagcatc agggcatccg cctgaccgcc tccgatactg aatgcctttc
    11701 cgtcgctccg ggtaccactg acagcggcac cggaatcggc cagcagtaca ccgccatttc
    11761 ccatggatac ggtggtggcg gtgccaccgt tgcggacatc cagcgttccg ccatcatcca
    11821 cgcgggtatt agtggctgtg tgtccggtca gcacatccag gcgtccgcca ttttccagta
    11881 cgacattatc agctttaccc tccacaacag agaatgctcc caggcggttt gtgccggtga
    11941 cggttgcagc tgtactggta accagtgcac cgcccgtgtt ctgggtgaca tcagacgctt
    12001 taccgccggc attcacctgc agctttcctt tctggttgat ggtggtatgc gcggcagttc
    12061 ctccttcctt aatcacctgc cagccatcac ggtttatcag cgtctctgtt gccgtgccac
    12121 cgttgtgtac atactggtta cccccctcca gtcgggtgtt atgtgcctct ccgtgtaccg
    12181 tctggtcacc accggcgtaa atgagggtgt cccgtgccat ccccgaagac cagataacct
    12241 gccggccatt tttattgatg gtggtggatt cagccgtccc tccgaccatc tgaccgctgg
    12301 acacattttc agactccgga ccaccttctg caccggtgtt gacgatggtt ccggttgcca
    12361 gtccgccatg ttttatggtc tggtaaccat cctggttaat aattgtaccg gcagctttcc
    12421 ctcccccgtg tacccactgc tcacctctgt tatccagcgt ggtgttcacc gccagtccgt
    12481 taaggctctg ccctccgcca tcacgaataa ccgtatcact ggcagttcct cctgcctgca
    12541 caatctgacg accatttgcg gtgacagtgg tgtttctgcc tgtgccaccc gcttttatcc
    12601 attgcccgcc ggtgttttcg tcactgtccg gccccagctc aagcccggta ctgacagtca
    12661 cgccatcagc tgttccggat acaaactggt tgtcatggtt taccagtgtt ccgccattca
    12721 ctgtttcacc cgggtgcaca acgatgtcag cagccagcac cgggagtgac gtgaccgcgg
    12781 caagagacag tgcaaccgcc acaccgccac gtttaccctg tgcgcgggcc agttcggagg
    12841 caaccacgaa agcgcccgta atgtgattcc ataccagcct gtagcaggta ttcagatgtc
    12901 gtttcatcag catttcctta atcagagtga gggtgaataa aaaagccggt atccacagat
    12961 gtggataccg gcaggtaaag ggttacagtg ccggaggtcc agctgacgga agtttctgac
    13021 gtgtggctct gttgttccct gaatctcatg tctgtgtgta tctgcgcacg ttgctaacag
    13081 gagttattat tagcataacg atcgatatta acgatcggta aaataggtat cgatcgtttt
    13141 attctattat cagtctattg cacggacaag agagacagta acgaaaacag cgcttttgtg
    13201 agcagactca gaggcaggga ggacatcatc cacaacaggt tcagaagaag atccagttcc
    13261 ataccgcgcg ggcaacagag accacggtgt cacgaacagc gcgcagggcc gtacggacaa
    13321 cagaggcaac acagacgctc tccgctgtgt caaatatccg gtccaccgca ccggtaaact
    13381 gttcacgggc ctgagagcgg acagactccg tgcgcagctc gtcctgcagt cgggtcatca
    13441 gggggctggc ggcatggtcg ggaagcgctg tcatgagcgc actgaccagc gtatccagtt
    13501 cccagccggt gcgggccgat acggcaacaa ccggatgtac gggccggaac agacggaata
    13561 ccgcctccgt tttctcacga atgttctgtt cctgtgcggg agaaggctga atacctgcca
    13621 tatcccattc atggcagggt tccgttttgt cagcctgcgt caccacaaac agcacctgct
    13681 gatgtccgca ctgcaggatg tgtcgccaga aatactcatc cacagacagg gcccggtcat
    13741 cggctttaat cagccacagc accaggtcca gttcaggcag aatgtcacgg tacagggctt
    13801 catactctgc atccctgtcc cggctctcgc ccaccccggg caggtcagtg ataaccatgc
    13861 tgtgaccgtg accactcaga cggaagcgct gcacttcccg ggtgccggcg tgaacatcac
    13921 tgaccggtgt gacctccccc tgaaacagtg cattacagag tgaagattta ccggccccgc
    13981 ttttacccat aatgccaatc acgggttcgt gactggtgag tttgcgcaga tgttccagga
    14041 tgtgacggga aagtgagtaa ggcagggagg agagcggttt ttcaattgcc tcaatggcat
    14101 cagacggatt catacatgta ttcccggtga aaaaagacaa aaccccgcgc accggaaagg
    14161 tggcggggga ttcacggaga taatatctgt ctgagattat ttagaatgta acaggtaatg
    14221 aattacttcc ttcgcttgtg ttcaagatat ttctctgtaa tcagcatttc aaaaaactct
    14281 cctgttctca gaccgtaaac agttctgagc tcatcaagca tttctttcac attgcttctt
    14341 atatgtatat taatggcctt tttaccatca ctccgatatt tgtccttttg ttttttctga
    14401 tgccaggcat gatttattcg tgttctgaac agttcaatgg tatcaggcgc ttctttccat
    14461 aaagcaaagg cgacaagaga ggcaagataa tattctttcg ttgaagatgg tatgaaccac
    14521 cgggtcactg ggttatgtac tggatgtttt tcatcctgtt tttgtttcag tcttgtccag
    14581 aaccactgac acatttcatc atcgtcagga aggagccact taaacttttg acaagcgctt
    14641 ttctttttaa gccacgcctg cctgagttcc tcgaggtata tgctttttgc atttttatta
    14701 attaaagaat ggcaggagtc aaaatatgaa atgattactt ttattcgttc gttatgggat
    14761 gatggattat ccggaaggtt actttttttg taagtgttta acgtttcatc ggtaactgtt
    14821 cgtattttcc tgacttcaat tccgtttttt attcctgatg aattatggtt tattttcggg
    14881 aattttgtat ggtcttcctt cgacagataa atagtagtcc aggtaaagaa agtcgcaagt
    14941 tcgtcagttt tcaaccagag aaaactctct tcaggcagta ttgcctgccc ggcaataatt
    15001 tcaagctcat taatttcgtt attgagtgct tcttcttcag tagtaccaaa agtaccaaag
    15061 gggggaggtg aaacattaca atacttttcc tttaaaaata aattaacatt cctgatacaa
    15121 ttttgtgggt tgtcaggcgc aaagtgaggg aattcattag ggtatttgtg attcagatat
    15181 tcggcataaa accgacattt acggggatct ctgcatttga taagcatctg ttcgtta
//
LOCUS       DAZXUL010000100        15114 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZXUL010000100 DAZXUL010000000
VERSION     DAZXUL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15114
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.100"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="ACH3L1_003126"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="ACH3L1_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFT4077775.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACH3L1_003127"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACH3L1_003127"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFT4077776.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="ACH3L1_003128"
     CDS             1531..2478
                     /locus_tag="ACH3L1_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFT4077777.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="ACH3L1_003129"
     CDS             2548..3354
                     /locus_tag="ACH3L1_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFT4077778.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="ACH3L1_003130"
     CDS             3364..3771
                     /locus_tag="ACH3L1_003130"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFT4077779.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4265
                     /locus_tag="ACH3L1_003131"
     CDS             3771..4265
                     /locus_tag="ACH3L1_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFT4077780.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWWPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="ACH3L1_003132"
     CDS             4331..5128
                     /locus_tag="ACH3L1_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFT4077781.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="ACH3L1_003133"
     CDS             5139..5927
                     /locus_tag="ACH3L1_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HFT4077782.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="ACH3L1_003134"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="ACH3L1_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFT4077783.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="ACH3L1_003135"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="ACH3L1_003135"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFT4077784.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="ACH3L1_003136"
     CDS             complement(8370..10001)
                     /locus_tag="ACH3L1_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFT4077785.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACH3L1_003137"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACH3L1_003137"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFT4077786.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="ACH3L1_003138"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="ACH3L1_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFT4077787.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3593 a         3985 c         3941 g         3595 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggtgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gaggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 cttaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attc
//
LOCUS       DAZXUL010000101        15020 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZXUL010000101 DAZXUL010000000
VERSION     DAZXUL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15020
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.101"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..2465)
                     /locus_tag="ACH3L1_003139"
     CDS             complement(<1..2465)
                     /locus_tag="ACH3L1_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFT4077788.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATS"
     gene            complement(2468..3028)
                     /locus_tag="ACH3L1_003140"
     CDS             complement(2468..3028)
                     /locus_tag="ACH3L1_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFT4077789.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(3154..3504)
                     /locus_tag="ACH3L1_003141"
                     /pseudo
     CDS             complement(3154..3504)
                     /locus_tag="ACH3L1_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(3707..4720)
                     /gene="intI1"
                     /locus_tag="ACH3L1_003142"
     CDS             complement(3707..4720)
                     /gene="intI1"
                     /locus_tag="ACH3L1_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFT4077790.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            4865..5362
                     /gene="dfrA12"
                     /locus_tag="ACH3L1_003143"
     CDS             4865..5362
                     /gene="dfrA12"
                     /locus_tag="ACH3L1_003143"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /protein_id="HFT4077791.1"
                     /translation="MNSESVRIYLVAAMGANRVIGNGPNIPWKIPGEQKIFRRLTEGK
                     VVVMGRKTFESIGKPLPNRHTLVISRQANYRATGCVVVSTLSHAIALASELGNELYVA
                     GGAEIYTLALPHAHGVFLSEVHQTFEGDAFFPMLNETEFELVSTETIQAVIPYTHSVY
                     ARRNG"
     gene            5474..5764
                     /locus_tag="ACH3L1_003144"
     CDS             5474..5764
                     /locus_tag="ACH3L1_003144"
                     /inference="COORDINATES: protein motif:HMM:NF017990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1010 domain-containing protein"
                     /protein_id="HFT4077792.1"
                     /translation="MFIQTAFSFSGVIQCLFCLFSGLRLHGLRRFSVFLASSPCVASA
                     SSYRFCSAVPPRWRSVFSRLAPVAKFKLSVLASGSNISVKPTRILRSAYLAR"
     gene            5770..6608
                     /locus_tag="ACH3L1_003145"
                     /pseudo
     CDS             5770..6608
                     /locus_tag="ACH3L1_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
     gene            6725..7072
                     /locus_tag="ACH3L1_003146"
     CDS             6725..7072
                     /locus_tag="ACH3L1_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFT4077793.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            7066..7905
                     /gene="sul1"
                     /locus_tag="ACH3L1_003147"
     CDS             7066..7905
                     /gene="sul1"
                     /locus_tag="ACH3L1_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFT4077794.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            8033..8236
                     /locus_tag="ACH3L1_003148"
     CDS             8033..8236
                     /locus_tag="ACH3L1_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077795.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            8392..9597
                     /gene="chrA"
                     /locus_tag="ACH3L1_003149"
     CDS             8392..9597
                     /gene="chrA"
                     /locus_tag="ACH3L1_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFT4077796.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            9608..9913
                     /locus_tag="ACH3L1_003150"
     CDS             9608..9913
                     /locus_tag="ACH3L1_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFT4077797.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            10140..10904
                     /locus_tag="ACH3L1_003151"
     CDS             10140..10904
                     /locus_tag="ACH3L1_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFT4077798.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(11397..11981)
                     /gene="mphR(A)"
                     /locus_tag="ACH3L1_003152"
     CDS             complement(11397..11981)
                     /gene="mphR(A)"
                     /locus_tag="ACH3L1_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFT4077799.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(11981..13219)
                     /gene="mrx(A)"
                     /locus_tag="ACH3L1_003153"
                     /gene_synonym="mrx"
     CDS             complement(11981..13219)
                     /gene="mrx(A)"
                     /locus_tag="ACH3L1_003153"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFT4077800.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(13216..14121)
                     /locus_tag="ACH3L1_003154"
     CDS             complement(13216..14121)
                     /locus_tag="ACH3L1_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFT4077801.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            complement(14243..14947)
                     /locus_tag="ACH3L1_003155"
     CDS             complement(14243..14947)
                     /locus_tag="ACH3L1_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFT4077802.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYISSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT         2999 a         4355 c         4496 g         3170 t
ORIGIN      
        1 gaggtggcca gccgcaggat ttcgtcccaa tgggcgcgga cgtgcttgat gttgagcgtg
       61 ccgccgatca tcggcttgag cgcgtcatag gcggcatcgc ccttcgggat gtagagcttg
      121 gtgtcgccca ggtcgcggat gcgcggcgcg aagcggaagc ccaagaggtg catcagggcg
      181 aagacgtgat cggtgaagcc cgccgtgtcg gtgtagtgct cctcgatccg caggtcggat
      241 tcgtggtaca gcaggccgtc gagcacgtag gttgagtcgc gcaggccgac attgaccacc
      301 ttggtgtgga atggcgcgta ttggtcggag atgtgggtgt agaaagtccg tcctgggctg
      361 ctgccatatt ttgggttgat gtgccccgtg ctctttgcct tgctagcggt tcggaaattc
      421 tgtccgtccg atgatgatgt ggtgccatcg ccccagtgcc cggcaaaggg atgccgaaac
      481 tgagcgttga ccagttcagc caacgctgtc gagtacgttt cgtcgcgggt atgccaggct
      541 tgcagccaag cgagcttcgc gtaggtcgtg ccggggcagg actcggccat cttggtcagg
      601 cccaggttga tcgcgtcggc caggatcgtg gtcaacaaca ggttcttgtc cttggccaga
      661 tcgcccgatt tcaagtgcgt gaagtgccgg gtgaagcccg tccactcatc gacttcgagc
      721 agcagttcgg tgatcttgac gtgcggcagg accatggctg tctggtctat cagcgcctgc
      781 gcggtgtcgg gcaccgccgc atccagcggc gtgatcttca agcccgactc ggtgatgatg
      841 gcatccggca ggtcgttggc tgccgccatg cggttgacgg tggcaagttg tgcttccagc
      901 agcgtcagcc gctcatgcag atattgttcg cagtcggtgg ccacggccag cggcaattcg
      961 ctggactgct tgaggctggt gaacttctcg ggcggtacca ggtagtcctc gaagtccttg
     1021 aactggcgtg aaccctgcac ccagatgtcg cccgagcgca gggagttctt caactcggac
     1081 agcgcgcaca gttcgtagta gcgccggtcg atgccggcgt cggtcatcac cagtttctgc
     1141 cagcgcggct tgatgaagcc ggtcggtgca tcggctggca gcttgcgggc gttgtcggtg
     1201 ttcatgccgc gcagcacctc aatggcatca agcacgtttt tggcggcggg cgcggcccgc
     1261 agcttgagca cggcaaggaa ttccggtgca tagcggcgca gggtggcgta gctctcgccg
     1321 atgcgatgca ggaaatcgaa gtcatcgggt tgcgcgagct tctgcgcctc ggtgacgctc
     1381 tcggcaaagg aatcccagga catgacggcc tcgatggcgg caaacgcatc gcggcctgat
     1441 tgcttggcgt cgatcagcgc ctgaccgatg cgcccgtaca gacgtacctt ggcgttgatg
     1501 gccttgcctg acgcctggaa ctgctgctga tgcttattct tggcagcgtt aaacagctta
     1561 cccaggatgc ggtcgtgcag gtcgatgatt tcgtcggtga cggtggccat gccctcggtg
     1621 gccagcgcca cgagagtggc gtagcgccgt tgcggctcga atttggccag gtcggcgggt
     1681 gtcatctggc cgccctcgcg ggcaatcttg agcaggcggt tctggtgaac cagccgctcg
     1741 atgccggtag gcagatcgag tgcctgccat gccttgaggc gttcgatgtg ttccagcata
     1801 tgccgcgaat ttggcttggc cggagactgg cgcaaccaag ccaaccaggt cgtcttgccg
     1861 ttgtcccggc gcttgagcag atcgtcgagg cggcggcgat gcgcgtccgc cagtggttcg
     1921 gccaaggcgt cgtagatgcg ccggttagca cgggtgatcg cctcggcact cgcccgctcg
     1981 acggcgttga gggcgggcag aatgaccgac tgccgccgca ggtgcccgat caaggcgctg
     2041 gccagcacga tgcctttgtc ggtttgcatc gccagctcgg tcagcatctg gacggcctgc
     2101 cggtaatggc tcatggtgaa gggccggaaa ccgaacacgg tttgcagctc gctcaggtgc
     2161 tcgcgccggg tctgctcccg ctggccgtac tcgttccagc tttcgacgcc gaccttgagc
     2221 tggtcggcga ccagcttcaa caagggcggg aacggtagtt catcgacgcc caggatgacg
     2281 ccgggaaagc gcaggtaaca gagctgcacc gcgaagccca gccgattggc tggcccgcgc
     2341 cgctgtcgga tgatcgagag gtcggtatcg ttgaatgtgt aatgtcggat caggtcgtcc
     2401 ttggagtccg gcaacgccag caggctttcc cgctcggcgg cggacaggat ggaacgacgt
     2461 ggcatattta ctgatccgtt ctcaagtatt gatacagggt ttcgcgactg attccgaatt
     2521 cacgagcaag cttggtcttt tgctcgccag cctcgacacg ttggcgcagt tcggcaatac
     2581 gctcagacga cagggatttc ttcctgccac ggtaagcccc gcgttgcttg gcgagcgcaa
     2641 taccctcgcg ctgacgctcg cggatcaggg cgcgctcgaa ctcggcgaac gcgcccatca
     2701 ccgagagcat caggttcgcc atcggagagt cttcgccagt aaaactgagg tgttccttga
     2761 cgaattcgat atgcacgccg cgttgtgtca gcgtttgcac gatccggcgc aaatcatcga
     2821 gattgcgcgc caggcgatcc atgctatgca ccaccacggt gtcgccggtg cgggcgaagc
     2881 ttatcagcgc ttccagttgc ggacgcttga catccttgcc ggatgccttg tcgctaaaag
     2941 cgcgatcaac cttgacgcct tccagttgcc gttccgggtt ctggtcgaag gtgctgaccc
     3001 tgatataccc aatgcgctgt ccagtcatgg aattccctgc aaaatgtcag ggaagactct
     3061 atgaccttca acgagatatg tcaataaatt caaaattcaa tcctatcctg acgcaattta
     3121 cacatggcat ctgacatcag gttagggtat gcctcaacct gacggcggcg aatcacaagc
     3181 gtccggtttg acgctggttt cggtcgcagt gctggcccgc gcctggaagc gctgatagca
     3241 ctccagcccg cagaaatgct ccacgtactc ggccccttcc ggcgtgaagg cggcatcgag
     3301 cgggatttcc ttgcagcaca cgcagcaact ggtgctcggt tcattggcgt tcatggtggt
     3361 gttcctccat tggttgacga agccgacgaa ggccgccgcc ggcatcggcc tggcgaacag
     3421 gaaaccctgc accgtgtcgc aacccgcctg ccgcaaccac gcaaggcagt cgggtgtttc
     3481 cacaccctcg gctaccacct ccattgtcgt tttcagaaga cggctgcact gaacgtcaga
     3541 agccgactgc actatagcag cggaggggtt ggatccatca ggcaacgacg ggctgctgcc
     3601 ggccatcagc ggacgcaggg aggactttcc gcaaccggcc gttcgatgcg gcaccgatgg
     3661 ccttcgcgca ggggtagtga atccgccagg attgacttgc gctgccctac ctctcactag
     3721 tgaggggcgg cagcgcatca agcggtgagc gcactccggc accgccaact ttcagcacat
     3781 gcgtgtaaat catcgtcgta gagacgtcgg aatggccgag cagatcctgc acggttcgaa
     3841 tgtcgtaacc gctgcggagc aaggccgtcg cgaacgagtg gcggagggtg tgcggtgtgg
     3901 cgggcttcgt gatgcctgct tgttctacgg cacgtttgaa ggcgcgctga aaggtctggt
     3961 catacatgtg atggcgacgc acgacaccgc tccgtggatc ggtcgaatgc gtgtgctgcg
     4021 caaaaaccca gaaccacggc caggaatgcc cggcgcgcgg atacttccgc tcaagggcgt
     4081 cgggaagcgc aacgccgctg cggccctcgg cctggtcctt cagccaccat gcccgtgcac
     4141 gcgacagctg ctcgcgcagg ctgggtgcca agctctcggg taacatcaag gcccgatcct
     4201 tggagccctt gccctcccgc acgatgatcg tgccgtgatc gaaatccaga tccttgaccc
     4261 gcagttgcaa accctcactg atccgcatgc ccgttccata cagaagctgg gcgaacaaac
     4321 gatgctcgcc ttccagaaaa ccgaggatgc gaaccacttc atccggggtc agcaccaccg
     4381 gcaagcgccg cgacggccga ggtcttccga tctcctgaag ccagggcaga tccgtgcaca
     4441 gcaccttgcc gtagaagaac agcaaggccg ccaatgcctg acgatgcgtg gagaccgaaa
     4501 ccttgcgctc gttcgccagc caggacagaa atgcctcgac ttcgctgctg cccaaggttg
     4561 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     4621 cggttggtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     4681 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     4741 ttgtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     4801 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag
     4861 ccatatgaac tcggaatcag tacgcattta tctcgttgct gcgatgggag ccaatcgggt
     4921 tattggcaat ggtcctaata tcccctggaa aattccgggt gagcagaaga tttttcgcag
     4981 actcactgag ggaaaagtcg ttgtcatggg gcgaaagacc tttgagtcta tcggcaagcc
     5041 tctaccgaac cgtcacacat tggtaatctc acgccaagct aactaccgcg ccactggctg
     5101 cgtagttgtt tcaacgctgt cgcacgctat cgctttggca tccgaactcg gcaatgaact
     5161 ctacgtcgcg ggcggagctg agatatacac tctggcacta cctcacgccc acggcgtgtt
     5221 tctatctgag gtacatcaaa ccttcgaggg tgacgccttc ttcccaatgc tcaacgaaac
     5281 agaattcgag cttgtctcaa ccgaaaccat tcaagctgta attccgtaca cccactccgt
     5341 ttatgcgcgt cgaaacggct aaccattccg tcaacgggac gccaaaatgc tgcgcatttt
     5401 ggttccctcc gctgcgctcc ggctctcgtt acgtccaacg ttagcaccac tgaaacccag
     5461 ctttatttag ctcatgttta ttcaaacggc atttagcttt tcaggcgtta ttcagtgcct
     5521 gttttgcctt ttttccgggc ttcgcctgca tgggctgcgc aggttttcag tctttttggc
     5581 ctctagccct tgcgtagcaa gcgcaagcag ctatcgtttt tgcagtgctg tgccgcctcg
     5641 gtggcgcagc gttttttcac ggttagcgcc cgtcgccaaa ttcaagttat ccgttttggc
     5701 ttctggttct aacatttcgg tcaagccgac ccgcattctg cggtcggctt acctcgcccg
     5761 ttagacatca tgagggaagc ggtgaccatc gaaatttcga accaactatc agaggtgcta
     5821 agcgtcattg agcgccatct ggaatcaacg ttgctggccg tgcatttgta cggctccgca
     5881 gtggatggcg gcctgaagcc atacagcgat attgatttgt tggttactgt ggccgtaaag
     5941 cttgatgaaa cgacgcggcg agcattgctc aatgatctta tggaggcttc ggctttccct
     6001 ggcgagagcg agacgctccg cgctatagaa gtcacccttg tcgtgcatga cgacatcatc
     6061 ccgtggcgtt atccggctaa gcgcgagctg caatttggag aatggcagcg caatgacatt
     6121 cttgcgggta tcttcgagcc agccatgatc gacattgatc tagctatcct gcttacaaaa
     6181 gcaagagaac atagcgttgc cttggtaggt ccggcagcgg aggaattctt tgacccggtt
     6241 cctgaacagg atctattcga ggcgctgagg gaaaccttga agctatggaa ctcgcagccc
     6301 gactgggccg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     6361 ataaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaataaa acgcctacct
     6421 gcccagtatc agcccgtctt acttgaagct aagcaagctt atctgggaca aaaagaagat
     6481 cacttggcct cacgcgcaga tcacttggaa gaatttattc gctttgtgaa aggcgagatc
     6541 atcaagtcag ttggtaaatg atgtctaaca attcgttcaa gccgaccgcg ctacgcgcgg
     6601 cggcttaact ccggcgttag atgcactaag cacataattg ctcacagcca aactatcagg
     6661 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     6721 tatcatgaaa ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacatc
     6781 cgcattaaaa tctagcgagg gctttactaa gcttgcccct tccgccgttg tcataatcgg
     6841 ttatggcatc gcattttatt ttctttctct ggttctgaaa tccatccctg tcggtgttgc
     6901 ttatgcagtc tggtcgggac tcggcgtcgt cataattaca gccattgcct ggttgcttca
     6961 tgggcaaaag cttgatgcgt ggggctttgt aggtatgggg ctcataattg ctgccttttt
     7021 gctcgcccga tccccatcgt ggaagtcgct gcggaggccg acgccatggt gacggtgttc
     7081 ggcattctga atctcaccga ggactccttc ttcgatgaga gccggcggct agaccccgcc
     7141 ggcgctgtca ccgcggcgat cgaaatgctg cgagtcggat cagacgtcgt ggatgtcgga
     7201 ccggccgcca gccatccgga cgcgaggcct gtatcgccgg ccgatgagat cagacgtatt
     7261 gcgccgctct tagacgccct gtccgatcag atgcaccgtg tttcaatcga cagcttccaa
     7321 ccggaaaccc agcgctatgc gctcaagcgc ggcgtgggct acctgaacga tatccaagga
     7381 tttcctgacc ctgcgctcta tcccgatatt gctgaggcgg actgcaggct ggtggttatg
     7441 cactcagcgc agcgggatgg catcgccacc cgcaccggtc accttcgacc cgaagacgcg
     7501 ctcgacgaga ttgtgcggtt cttcgaggcg cgggtttccg ccttgcgacg gagcggggtc
     7561 gctgccgacc ggctcatcct cgatccgggg atgggatttt tcttgagccc cgcaccggaa
     7621 acatcgctgc acgtgctgtc gaaccttcaa aagctgaagt cggcgttggg gcttccgcta
     7681 ttggtctcgg tgtcgcggaa atccttcttg ggcgccaccg ttggccttcc tgtaaaggat
     7741 ctgggtccag cgagccttgc ggcggaactt cacgcgatcg gcaatggcgc tgactacgtc
     7801 cgcacccacg cgcctggaga tctgcgaagc gcaatcacct tctcggaaac cctcgcgaaa
     7861 tttcgcagtc gcgacgccag agaccgaggg ttagatcatg cctagcattc accttccggc
     7921 cgcccgctag cggaccctgg tcaggttccg cgaaggtggg cgcagacatg ctgggctcgt
     7981 caggatcaaa ctgcactatg aggcggcggt tcataccgcg ccaggggagc gaatggacag
     8041 cgaggagcct ccgaacgttc gggtcgcctg ctcgggtgat atcgacgagg ttgtgcggct
     8101 gatgcacgac gctgcggcgt ggatgtccgc caagggaacg cccgcctggg acgtcgcgcg
     8161 gatcgaccgg acattcgcgg agaccttcgt cctgagatcc gagctcctag ggatcgcctc
     8221 agaaaacgga aaataaagca cgctaagccg taagtaagcg tgctcctgtg aaagccacag
     8281 ctaaaactgc gtagtacaca tagaggtatt tttttctaga tttatatcga atttcgttat
     8341 cgaatttcgc ttattattga tcaactcgtt gcagatcatg aaggcgaaag tatgacgaat
     8401 aaccctaccg atgacagcag accatggtcg gtctttctta tttttctgcg gcttggattg
     8461 acatcttttg gcggccccat tgcgcacttg ggctacttcc gcgccgaatt tgtcacacgg
     8521 cggcgctggc tctccgaacg gagctatgct gacttggtcg cgctttgtca gttcttgcca
     8581 gggcctgcaa gcagccaggt cggcatagcg gtaggactgt ctcgggctgg atacagcggg
     8641 gcgctggctg cttgggctgg cttcacgctg ccgtctgcca tagccttgat cctttttgcg
     8701 ctcggcatct ccagctatgg cgattacgtc tcgcagggcg cgttgcatgg cttaaaagtg
     8761 gtggctgtgg ccgtggtcgc tcaagcagta tggggcatgg cgcgtaacct atgcacggat
     8821 gggctgcgag tcaccatcat ggcaattgct acctgcgtcg ttttacttgt gccgtccgcg
     8881 tggggacagg ttggcgtgat tgctatcgca ggcatcgcag gccggttatt gttcaagcca
     8941 gcgaaagttg ttgagcatga ccccctacct atcacggtca gtcaccgggc cggcgtgctt
     9001 tggctctcgc tgttctttgt cttgctgatt ggcctgccgg tgttggccga actgatgcca
     9061 agtcaaacca tggcaatggt ggattccttc tatcgtgtcg gatcactggt gttcggcggt
     9121 ggtcacgttg tgctgccatt actgcaagcc gaagtggtgc cctccggctg ggtcaacaat
     9181 gaatcctttc tcgcggggta cggggcagct caagcggtgc ccggcccttt gttcacgttc
     9241 gccgcgtttc ttggtgcctc gatgaacacc gccccgtcgg gctggatcgg cggcattgtg
     9301 tgtctgctgg ctatcttcgc gccctcgttc ttgctggtcg tcggatcaat gccattttgg
     9361 gagcgtttgc gccgcaatac aggcatccaa gctgcgctgg ccgggatcaa tgccgctgta
     9421 gtcggcttgc tgctggccgc gctgtatcag cctgtatgga ctagcgccat ctttcagccg
     9481 caagacttcg gcttggcatt agttgccctt gtcgcactta tgttctggaa gctcccgccg
     9541 tggctggttg tcctcggtag cggtgcagct gggtggctgt tgagcgtcgc tctgtgaggc
     9601 acaaaaaatg actgacaaag acctctacgg cggtttgatc cgcctgcaca tccttcacca
     9661 tgcagccgag gaacctgtct ttgggctggg gatcatcgaa gagctacgcc gacacggcta
     9721 cgagatgagc gctggcaccg tgtacccgat gctgcacggc ctggaaaaaa aaggctatct
     9781 gacctcacgc cacgaacgca ccgggcgacg tgaacggcgt gtttatgaca tcactgaaca
     9841 aggcagaact gcacttgctg atgcgaaaac aaaggtcaag gagctgttcg gagagttggt
     9901 agaaggtggt tgagtttttg cttctatgtc aggttgagct ataccctaac tggattgtca
     9961 ttttcagaag acgactgcac cagttgattg ggcgtaatgg ctgttgtgca gccagctcct
    10021 gacagttcaa tatcagaagt gatctgcacc aatctcgact atgctcaata ctcgtgtggg
    10081 ctctgttgca aaaatcgtga agcttgagca tgcttggcgg agattggacg gacggaacga
    10141 tgacggattt caagtggcgc catttccagg gtgatgtgat cctgtgggcg gtgcgctggt
    10201 attgtcgcta tccgatcagc tatcgcgacc ttgaggaaat gctggcggaa cgcggcattt
    10261 cggtcgacca tacgacgatc tatcgctggg tccagtgcta cgccccggag atggagaagc
    10321 ggctgcgctg gttctggcgg cgtggctttg atccgagctg gcgcctggat gaaacctacg
    10381 tcaaggtgcg gggcaagtgg acctacctgt accgggcagt cgacaagcgg ggcgacacga
    10441 tcgatttcta cctgtcgccg acccgcagcg ccaaggcagc gaagcggttc ctgggcaagg
    10501 ccctgcgagg cctgaagcac tgggaaaagc ctgccacgct caataccgac aaagcgccga
    10561 gctatggtgc agcgatcacc gaattgaagc gcgaaggaaa gctggaccgg gagacggccc
    10621 accggcaggt gaagtatctc aataacgtga tcgaggccga tcacggaaag ctcaagatac
    10681 tgatcaagcc ggtgcgcggt ttcaaatcga tccccacggc ctatgccacg atcaagggat
    10741 tcgaagtcat gcgagccctg cgcaaaggac aggctcgccc ctggtgcctg cagcccggca
    10801 tcaggggcga ggtgcgcctt gtggagagag cttttggcat tgggccctcg gcgctgacgg
    10861 aggccatggg catgctcaac caccatttcg cagcagccgc ctgatcggcg cagagcgaca
    10921 gcctacctct gactgccgcc aatctttgca acagagcccg ccgtgctagt ctgctcggtg
    10981 atggtggagt gaagccaacc cgcaatcggg ttatgaatct gcatcgcgat tcgcaatcag
    11041 ctgtctcttg agcatgtcga actcctgcga tgttatctcg ccgtgctcgt gctgctacac
    11101 caacttgtcc aactcatccg ccacaccgct ccccaccacc tgcgatggcc aggttgtttg
    11161 ctgggcgcat tggcggagta tcgtctcgac ccgggacacc cacaccggca caaccttacg
    11221 ggagtgattc actgtcaaag aatcggcccg gtgctctgac gcaagtatcg ggatggtcac
    11281 catttgtaag ccgtagacct gagtggtgat caagacttcg ataccaccga ccgtaccggt
    11341 actaatcgac gacggtcgtg ttcgtcgcct gccgcaggga ctctgcacac ctccgtttac
    11401 gcatgtgcct ggaggagttg gaaatcgtcg tgttcgggaa acattaaaca caggatggca
    11461 gcgatctgag ccagcacatg atcagctagc tcaccatccg gatcgacggc ccactgcatc
    11521 gtcgcgccag cgatgaccga gtgcaggagc aactcagctg ccgcaggagc acctgggggc
    11581 agtcgcttgc ggatcccctc caccaccgcg cggttccgct ggatcgcaag cgtgcgtagc
    11641 tccggcacct ggagctcgta ccaggagatg agatagttca ccgagaagtc gttgcgagtg
    11701 ttcatgctcc gaacgagcac ctgcaaaaat tcccagagcc cttgcggccc tgcgcctatc
    11761 ggtatcgcat tcaggtaatg ccgcacctgc tcgacgccgc gctccatcat cctcaccagc
    11821 agcgtatcgc ggttggtgaa gcgctggatt aacgctgcgc gggagagccc cacctccttt
    11881 gctactccgc tgagcgtgaa ctctatggga ccgcaacgct tcagcactac ggtggcggcc
    11941 tcgagtacct cgtcatcgga cttgagcttg gggcggggca tcagtgttca ccttctgtat
    12001 gggttggggc ggaggctgtg gctgccgccg ccattgtagc aaattgaaga cggagcgaga
    12061 gtagagccac gagccccgca aacacggccg atacaacgag gccagggagc ggaccagcaa
    12121 ggtcgacaaa cgcgccggcc gcaagcataa ccatgggcga ggctgacagc atcaccgccg
    12181 agaccgtgcc gagtacccgg ccgagaagtt ctggcggcgt gcggttgtag atggcagcgt
    12241 tgagaatggg agagactgag ccggtcagca gtcccacgag cgcgcccaac aacatcagca
    12301 ccggcacgcc tggcaactgt gaaagcagaa gcgagcccac cgcagagcca caaaatgcca
    12361 ccgccagcca gttctgcgct gatatccggg cgccgaccga cgcatgaatg gcaatgccaa
    12421 ggagaccacc agcccccatc attgaggaga acagcccgag ctctgctact tggcgtcctg
    12481 catctacaaa cagcgcaggc atgatgacgc tgccgttggc gccaacgatg cccacgaaga
    12541 tcatcactat accaaagaga gggcgcagca ggggttcgct ccagagaaaa gcgacgccgg
    12601 cgcgcatgga gagagtcgcc gtcgtggtca tcgtccgagc ggcacgcgcg ggaagcaccc
    12661 acgcgccgag cagacctgca aggacggagc agaacgccgt cagcccgagc gttggcgcag
    12721 cgccaagcag gccgattgcg gcccccccaa gggccgggcc acctagaatc gcgacgttcc
    12781 cgatcaccgc tttcagtgac gagacgcgct caacggagag cccggcgacg tggccgagtt
    12841 tgggcagctc actgtcctgc gcggccatac cgggtgcgtc gaacgcggca ccgagcacca
    12901 cgcaagcgat cagcccagtg ttcgagaggg cgccaacggc atcgagcagt gggatgctcg
    12961 ccatggccac gccgcccacc acacccgaga tcaatgcgac gggcgcgcgc ccgaaccgat
    13021 cgacgaggcc accaccaacc cacgcgccga tgatggtcgc gatgacgctg ctagcggccg
    13081 tggcgcccgc ccaggccgcg ctctttgtat gagacaggac gaaccatgga agcgcgaggg
    13141 ccgccaccgc gttgccgatc cggaagagaa aggtcgccgc gaacagcgtc gcgagcgggc
    13201 tatatcgacg ttcgctcatt ccgctgcggc gagctgcgcc ttcgccgcag cgaggtactc
    13261 ttcgttaccc gagtcgaggg cgaagagtgc gtaggtgacc gccccgaacg caaggcgctc
    13321 cgcgatgtgg tgggcgagcc gcggccacac ccggccaccg gccgcttcat acgtgaggag
    13381 gagcttcgcg agcccctctt caccaaagac cataaggtgc gcggccatgt cgatggcagg
    13441 gtcatcaacg cgggcctcgc tccagtcgat catcccgctg acgcgctccg tgttgtcgat
    13501 gagcacatgg cccacgtaga gatcgccatg caccaccacg gagaaatctg gccacgacga
    13561 atcgtcgtcg agccagcgct gccaccggtg gaggcgcttg tcgttcacca cgaactcgcg
    13621 tcggacgcgg tcaacgtcgt cggccacctt ctgacgggcc tgcgtcggtg tacggatgag
    13681 catccccgca tccacggcgg cggaaatggg gacggcatgc agggcggcga gcgcggtcgc
    13741 gaagctctcc gcgaagacct ccgagtcctg cggcacgacc cagtcgggcg tggacgaacc
    13801 aggctggatg accatcgcag tcgagtcttc gagcatggga taggcaacga gctcggcgtt
    13861 ggccacgcgc cagtccggca ccgcgaacgg caggcgattc ttgagcattg ccagcacccg
    13921 cgcctctggt tcgaccttcg cgcttacctc ggctcggcgc gggatgcgca gcacccaccg
    13981 acgtccatcg tcgacggtgg cgatcacgat cctatagtcg agcccaagct cattgacagt
    14041 cagcgggcca tggagcttga gcccatgtcg ggctgcaagt gcgtacagtt gggaggtatc
    14101 ggcggtcgtg actacggtca tgattcactc ctgagggctt gacgggttta gccacctaaa
    14161 tataacagtc acgtcggtta tattcaatcc ggcactgttg caaagttagc gatgaggcag
    14221 ccttttgtct tattcaaagg ccttacattt caaaaactct gcttaccagg cgcatttcgc
    14281 ccaggggatc accataataa aatgctgagg cctggccttt gcgtagtgca cgcatcacct
    14341 caataccttt gatggtggcg taagccgtct tcatggattt aaatcccagc gtggcgccga
    14401 ttatccgttt cagtttgcca tgatcgcatt caatcacgtt gttccggtac ttaatctgtc
    14461 ggtgttcaac gtcagacggg caccggcctt cgcgtttgag cagagcaagc gcgcgaccat
    14521 aggcgggcgc tttatccgtg ttgatgaatc gcgggatctg ccacttcttc acgttgttga
    14581 ggattttacc cagaaaccgg tatgcagctt tgctgttacg acgggaggag atataaaaat
    14641 cgacagtgcg gccccggctg tcgacggccc ggtacagata cgcccagcgg ccattgacct
    14701 tcacgtaggt ttcatccatg tgccacgggc aaagatcgga agggttacgc cagtaccagc
    14761 gcagccgttt ttccatttca ggcgcataac gctgaaccca gcggtaaatc gtggagtgat
    14821 cgacattcac tccgcgttca gccagcatct cctgcagctc acggtaactg atgccgtatt
    14881 tgcagtacca gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg cctttgaatg
    14941 ggttcatgtg cagctccatc agcaaaaggg gatgataagt ttatcaccac cgactatttg
    15001 caacagtgcc catcggcgta
//
LOCUS       DAZXUL010000102        14978 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZXUL010000102 DAZXUL010000000
VERSION     DAZXUL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14978
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.102"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..104)
                     /locus_tag="ACH3L1_003156"
     CDS             complement(<1..104)
                     /locus_tag="ACH3L1_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077803.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLN"
     gene            complement(520..1461)
                     /gene="fabY"
                     /locus_tag="ACH3L1_003157"
                     /gene_synonym="yiiD"
     CDS             complement(520..1461)
                     /gene="fabY"
                     /locus_tag="ACH3L1_003157"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFT4077804.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1506..1943)
                     /gene="dtd"
                     /locus_tag="ACH3L1_003158"
     CDS             complement(1506..1943)
                     /gene="dtd"
                     /locus_tag="ACH3L1_003158"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFT4077805.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1940..2812)
                     /locus_tag="ACH3L1_003159"
     CDS             complement(1940..2812)
                     /locus_tag="ACH3L1_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFT4077806.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2806..3405)
                     /gene="yihX"
                     /locus_tag="ACH3L1_003160"
     CDS             complement(2806..3405)
                     /gene="yihX"
                     /locus_tag="ACH3L1_003160"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFT4077807.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3504..4289)
                     /locus_tag="ACH3L1_003161"
     CDS             complement(3504..4289)
                     /locus_tag="ACH3L1_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFT4077808.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDGLPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4323..5219)
                     /gene="yihV"
                     /locus_tag="ACH3L1_003162"
     CDS             complement(4323..5219)
                     /gene="yihV"
                     /locus_tag="ACH3L1_003162"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFT4077809.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5387..6283
                     /gene="yihU"
                     /locus_tag="ACH3L1_003163"
     CDS             5387..6283
                     /gene="yihU"
                     /locus_tag="ACH3L1_003163"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFT4077810.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6307..7185
                     /gene="yihT"
                     /locus_tag="ACH3L1_003164"
     CDS             6307..7185
                     /gene="yihT"
                     /locus_tag="ACH3L1_003164"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFT4077811.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7202..8443
                     /gene="yihS"
                     /locus_tag="ACH3L1_003165"
     CDS             7202..8443
                     /gene="yihS"
                     /locus_tag="ACH3L1_003165"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFT4077812.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8581..9483
                     /locus_tag="ACH3L1_003166"
     CDS             8581..9483
                     /locus_tag="ACH3L1_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFT4077813.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9682..11718
                     /gene="yihQ"
                     /locus_tag="ACH3L1_003167"
     CDS             9682..11718
                     /gene="yihQ"
                     /locus_tag="ACH3L1_003167"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFT4077814.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11764..13138
                     /locus_tag="ACH3L1_003168"
                     /pseudo
     CDS             11764..13138
                     /locus_tag="ACH3L1_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13181..14584
                     /locus_tag="ACH3L1_003169"
     CDS             13181..14584
                     /locus_tag="ACH3L1_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4077815.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14652..>14978
                     /locus_tag="ACH3L1_003170"
     CDS             14652..>14978
                     /locus_tag="ACH3L1_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFT4077816.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3673 a         3856 c         3736 g         3713 t
ORIGIN      
        1 tgatttaata gcgtttgctt cggtgattta ttactcatta gttgtttttc taaattaaca
       61 acagaataac tttcttgttt aatgagatta atattggcaa ccattatagc ctccttccca
      121 cggtttaaga gcaaacatac aacataatga ttcataacat acagagcagt aaatgaatga
      181 tactgacaat gtatgccagc ccattatctt tgcataatat ttgtgagcta tagattttaa
      241 aaatatttat gaaaaaagta tggcaccaga gggggttttg actatcaaaa atcgctcaaa
      301 cctgaactaa tatgatgagt taagcattat ctgcaataat tgccactcag ctctacaaat
      361 aatatttaaa tgctcctgaa cagtgatgta gcaagacacg tatggtgcca acgaagaata
      421 aaaatttata aagttcttat tcaatataac cggaatagtc agggactgat gtaaatgacc
      481 ggaatgaatg agagaggcag caccgcccct ctccataagc tactcttctt cgttcccgcc
      541 ctcttcatac gggccaaatg gcttcgcggg cagaacgata tacgtgcctt caaacactgc
      601 acccggcgtc tcgtcgccaa agatttcgac ctgcatctgc acccgtgctt ttcgtccgcg
      661 cgccagacgg tcgagatcgc cgcttaaggc accaaggtcg gctaccgcat ggggtttacc
      721 gctaatcggt ttgctgtagc ggatatgcgc atccgccaga ataatcgttc cgccgaggtg
      781 gcgttcacgc agcatcagcc agataagccc ccaaccggtg agcgtcgcca gcgagaataa
      841 actcccggca aacagcgtat ggtgcggatt ctgattgccg gtttccggca tggtggtgat
      901 aaatttttgc ccggtatatt gttgaatacg cacgcccatt ttttcactaa gcgggatatg
      961 ttcgtaccac gcctgttgca gctgcgcgca ccagtcgccg cgatgcaaaa tatcatccag
     1021 agtggcgacg ggcttaatca tcaaaaaatg gcgaatcggc gtggtggttg gcgtggtgat
     1081 ttctccctga ttaacaaacc ccagcttggc gaaaaactcc accgcgtctt cacgggcgct
     1141 acaggtcacg cgcttaacgc cttcctgacg cgccaccgac tccagggtca tcgccatcag
     1201 cgttcctaac cctttgtcct gcacgtccgg gtgaacgacc ataaagcgaa tggacgcttc
     1261 attgtcggca ttaatataca atcggcctac cgccaccaga ttaccctgct cgtcgacgac
     1321 catctggtga tgcgccatcg catcccacgc gtcgcgttcc gaaccttttg gttgatgcag
     1381 gggcttacgc aacatttccc agcgaaagtg atagtaacgc tctaactctt cttctgtttg
     1441 tggaacctga aggtgataca tagcgatact ctctcttgtt tcccgtgaca accctggaag
     1501 ctggctcata cctgcaacca gaatgtcacg gggccatcat tgaccagcga tacctgcata
     1561 tcggcagcga agcgtcctgt ttgcgtgttc atctcttgct ggcggcagcg ttcgacgaaa
     1621 tagtcatata acgcctctgc gcgatccggt gatgcgcctt tggagaaact tgggcgcatc
     1681 ccccgttcgg tatctgcggc gagggtaaac tgggaaacca ccagtacact gccgcccgcc
     1741 tgttgcacgt tgagattcat cttgccttcg gcatcgctaa agatgcggta gccgagcaca
     1801 cgctcgcaca gacggtttgc tttctgttcg tcgtcatcct tttcgacacc caataacacc
     1861 aaaagtcccg cgccaatttc gcccgtcact tctccctcca cggtgacgct ggcacgggtt
     1921 acgcgttgaa ttaatgcaat catggttcgt cgtcttcttc ttgttcagct gcttgtttta
     1981 gtttgcggta ttccccgaga gtgacagtaa tttccgcgcc aagcaagacg atacaccatg
     2041 tccagtagac ccaaacaaag agaatgggga tcaccgccag cacaccgtaa atgagctgat
     2101 atgacgggaa catggtgata taaagcgcga aacctttctt tcctgcttcg aacaggagtg
     2161 cggcgacaaa cgcgccgaca atcgcgtcgc ggttaggtac gcggatggta ggaacaatgc
     2221 tgtacagcaa ccagaaggag atccacgaca acagcaacgg aaaaatacgc agcacgttgt
     2281 cgatgacagt attgagatcg ctcgcccagc gcagggagag caaataggaa ctgatcgcca
     2341 gactggcccc tgccagcagc ggccccagcg ttaaaatcat ccagtacacg gcgaacgagt
     2401 aaatttttgg tcgcgctcgt ttactgcgcc agatggtatt caacgcgcta tcgatggagt
     2461 acatcaacaa taacgcggtg acgatcagcc cgcacgcccc aacggcggtc atcttgttgg
     2521 aattagcaac aaattgttcg atataccgct gaataacatc gccagtagca ggcagaaagt
     2581 tggcaaaaat aaagtgacgt aactgaatgc tgacgtcgga aaacatggga aaagcggcga
     2641 aaagcgcaaa aacaacggca accagcggca ctaatgagag caacgacaca taggcaaggt
     2701 tacctgccag ggttgtcatg ttgtcctcat caatacgttg ccagagtagt tttagccagg
     2761 cccatagtgg acgggtacgg tgcctggctt tgtcctgaat ggtttttagc ataacacctt
     2821 cgcgaaatag tccgggatgg tggttttatc tttcaccaga atactggtaa tgcctagctg
     2881 attagctcct tctatattat cggcgttatc gtcgaaaaag accgtatcgc tgggtgaaaa
     2941 accttccgcc tgcaaaacat gctggtaaat tcgtgcttca ggtttgcgca tccccagatc
     3001 ttgcgacaga tagatatggt cagcagcatc acgaatttcc gggtattctt ccggccagaa
     3061 ggtggtatgc aggcggttgg tattggaaag caccaccacg cgatgcccct gctcacgcag
     3121 tttatgcatg atggcgatca cttccgggcg cagcgcaaca aacaccgcct gccagccgtg
     3181 agagaactgc tcgtagctta gcggtagagc catctcatga cacagcgcct ctgcgaacgc
     3241 ttcgtcgcta atttccccac gctcatgctg atgaaacgcc tcccccatat gaaaactctt
     3301 cttaagcgat gccagcggaa tacgcgttaa atcgctccag gctcccagca cacggttaaa
     3361 gtcgatatcg acaatcacat tacctaaatc aaagatatag agcatttttg cctcctttcg
     3421 cgccacaaga aattaactgt agcgggaaag gatgggtttg gctatgtggc aaggtatgaa
     3481 gccaggttaa cggtcacaaa aatctacgcg tcttcctggg gaagaatgat ttcaatttgg
     3541 ctgtcttgta agcgtgattt tagcgcagcg ggcggcagcc cgtcggtaaa gagcgcagtg
     3601 acctgtgcca cgttaccaat ttcaaccgcc gccgaagcat gatacttagt gtgatcggcg
     3661 accagcagaa tatttctcgc gtgcgccatc atcgttttca ccacgttagc ttcgtttaca
     3721 tcaaactcca tcaacgcgcc atcgctctca atcgccccaa cgcttgttac cagataatca
     3781 gcacgaaaat cagccacaaa ggacgccgct gaagggccaa tgatcccgct attatgagag
     3841 cgcaacgtac cgccgggcac catcacttca aagcgcgggt tgtggtaaag aatatgcgcc
     3901 acacgcaggc tgttggtgat tatccgcaaa tgattatggt taagtaacgc ccgggcaaca
     3961 tgctcaacag tcgtaccaat ggtgataaat attgttgaac catcaggaat atagtctgcc
     4021 accgcttcgg caatcgcttt ttttttctcg gtttgcgaaa cctcacgctg ctcgaacgcc
     4081 gtattaacga cgctggaagc ccgacccgcg ccaccgtgat ggcgcgtaat caggccttgc
     4141 tcgcttaatt tacgaatatc ccggcggacc gtctgcgtgg agacatccag cagatttgcc
     4201 agctcatcaa tattcatata cccacgctcg gcgatcagca tgaggagttg gtcgtgccgc
     4261 gggttaccgg ttagttcggt aaggctcatg aaaattcctc gaaaaaccat caccctggca
     4321 ttttatacaa aaagtgacaa aaaagatcgg gtttgatcac agtcagggat cccggctcgt
     4381 ccaccgggac gtgtgcattt taacgccgct acaccgctgg cgaagcggac tgactccgct
     4441 aaatccccac ttgttgccag cgccaccgcc aaagcgccgt gaaaaacatc tcccgcacct
     4501 gtggtatcta ccacatcaac tttgaaggcc ggttgatgct gacgcccacc attttccagc
     4561 cagtcgcagc ctgcgctacc ctgggtcaca tagacatgac catttgtgag cgtttgtgcc
     4621 tgttttagcg cactggccat ctctttcacg cccgttaagc gcgccagacc cggttctgaa
     4681 aaggccgcgt gatcgcttaa tgccaccagc tcactgatat cctgcggcgt aatgtccccg
     4741 tccagaacgg tcatcacacc cgcctgacgg gccagggtga aggctttttt agcgccgtcg
     4801 tgccagcgta catctgccag cacaacatcc cactgagaga aatcaatttc ctccaaccac
     4861 tctgcgtcag gcagcagatc cgggctgggg tagttaatga ttatccgctc gcctttggta
     4921 tccaccatga tggcggattg cgaagatttc gcctggttat accgtttggt gtaacgggtg
     4981 ttaacgcccc aggattccag ctctgccagc aggctgttgc ccgcgtcgtc atcacctacg
     5041 cgaccaataa aatcgacctg cgcccccagc cttgccgccg caaccgctgc cgtcgccgct
     5101 ggcccgccac caacttccgt ataatttctc gccacgtatt taccgctctc cgtcggtaac
     5161 ccttccacgt aatagatgcg atccatcacg gttataccta cacaagcaac acgaatcatg
     5221 gtcattcctt aaacattttg ggatgcgttc attttaattt caccaattgt ttaaaaagtg
     5281 acggctgtca attttttgac gataaattac aaattcgatc aaaaacagac aagcaaagca
     5341 tgtcataaat gacaaaaagt gacatcactg tattcaggag agggttatgg cagcaatcgc
     5401 gtttatcggt ttaggacaaa tgggttcgcc aatggcgagc aatttattgc agcaagggca
     5461 tcaacttcgc gtctttgatg tgaacgccga agctgtgcgg catctggtag acaaaggtgc
     5521 gactcccgcc gccaacccgg cgcaggccgc taaagatgcc gaatttatca ttaccatgtt
     5581 gccgaatggc gatctggtgc gcaacgtgtt gttcggtgaa aacggcgttt gcgaaggctt
     5641 atctaccgat gcgctggtca ttgatatgtc caccatccat ccgctgcaaa ccgataaatt
     5701 gattgccgat atgcaagcca aaggcttcag catgatggat gttccggtag gccgtacttc
     5761 tgcaaatgcc attaccggta ctctgttact gctggctggc ggcaccgctg aacaagttga
     5821 acgtgccaca ccgatcctga tggcgatggg cagtgagttg atcaacgctg gcggtccggg
     5881 catggggatc cgcgttaagc tcatcaacaa ctacatgagc atcgcgctca atgcgctttc
     5941 ggcagaagcc gccgttttgt gcgaagccct gaatcttccc ttcgatgttg ccgtcaaagt
     6001 gatgagcggt accgccgccg gtaaaggtca cttcaccact tcctggccaa acaaagtcct
     6061 cagcggagat ctttctcccg ccttcatgat cgatcttgcc cataaggatc ttggcatcgc
     6121 ccttgatgtc gccaaccagc tgcatgtgcc aatgccgctg ggggccgcct cacgggaggt
     6181 ttatagccag gcgcgcgcag cgggtcgcgg tcgccaggac tggtccgcca ttctggaaca
     6241 ggtccgtgtc agtgccggga tgactgccaa agtaaaaatg taacgactgg ataaaggaat
     6301 aaatgaatga ataagtacac catcaacgac attacgcgcg catcgggcgg ttttgccatg
     6361 ctggcggtcg atcagcgcga agccatgcgc atgatgtttg ccgcggctgg ggcacccgct
     6421 ccggtagccg atagcgtttt aactgatttc aaagttaacg ctgcaaaggc cctctcgcct
     6481 tatgcctcgg cgattctggt agatcaacaa ttctgctacc gccaggtggt tgagcaaaac
     6541 gcgattgcca aaagttgcgc catgattgtc gccgccgatg agttcattcc tggcaacggt
     6601 attccggtcg atagcgtggt tattgaccgc aaaatcaatc cgctacagat caaacaagac
     6661 ggtggcaaag ccttaaaact gctggtgctg tggcgtagtg atgaagatgc gcagcaacgt
     6721 ctggatatgg tgaaagagtt caacgaactg tgccactcac acggtctggt aagcatcatt
     6781 gagccagtcg tccgcccacc gcgtcgtggc gataaattcg atcgcgaaca agcgatcatc
     6841 gatgccgcca aagagctggg cgacagtggc gctgacctct acaaagttga aatgccgctt
     6901 tatggcaaag gtccgcaaca agagcttctc tgtgcttcac aacgtctgaa tgaccatatc
     6961 aatatgccat gggtgatcct ctcttccagt gtcgacgaaa aactgttccc gcgtgccgta
     7021 cgcgtggcaa tgacggcggg cgcatcggga ttcctggcag gccgtgcagt ctgggcatcg
     7081 gtcgtcggtt taccagacaa cgagctgatg ctccgtgacg tttgcgcacc gaaattacaa
     7141 caacttggcg atatcgtcga cgaaatgatg gctaaacgcc gctaagatga ggataaaagg
     7201 aatgaaatgg tttaacaccc taagccacaa ccgttggctg gaacaggaaa ccgaccgcat
     7261 ctttgatttt ggtaaaaatt ccgtagtgcc gactggtttt ggctggttag gcaataaagg
     7321 gcaaatcaaa gaagagatgg gcacccatct gtggatcaca gctcgtatgt tgcacgttta
     7381 ttccgttgct gcggcgatgg gtcgacctgg cgcttacgcg ttggttgatc acggtattaa
     7441 agccatgaac ggcgcactgc gtgataaaaa atatggcggc tggtatgcct gcgtgaatga
     7501 cgagggcgtg gtggatgcct ccaaacaggg ctatcaacac ttctttgctc tgctgggtgc
     7561 cgccagcgcc gtcacaacgg gtcacccgga agcgcgcaag ctgctcgatt acaccattga
     7621 gattatcgag aaatatttct ggagcgaaga agagcagatg tgcctggagt cctgggacga
     7681 agccttcagc aaaaccgaag agtaccgcgg cggcaatgcc aatatgcacg cggtggaagc
     7741 tttcttgatt gtttatgacg tcactcacga caaaaaatgg ctggatcgcg cgattcgcgt
     7801 ggcttccgtg attatccacg acgtcgccag aaataatcat tatcgcgtta acgaacactt
     7861 cgatacccag tggaatccgc tgccggatta caacaaagat aacccggcgc atcgcttccg
     7921 cgcgttcgga ggtacaccag gccactggat cgaatggggc cgtttaatgc tgcacatcca
     7981 tgctgccctg gaagcccgtt gcgaacaacc accagcatgg ctgctagaag atgccaaagg
     8041 tctgtttaac gccaccgtgc gcgatgcctg ggcacccgat ggagcggacg ggattgttta
     8101 taccgttgac tgggaaggaa aaccggtggt ccgcgaacgt gtacgttggc ctatcgtcga
     8161 agcaatgggt acggtctacg cgctctacac cgtcactggc gatcgccagt atgaaacctg
     8221 gtatcaaaca tggtgggagt actgcattaa gtacctgatg gactatgaaa atggttcctg
     8281 gtggcaggag ctggatgcgg acaataaggt catcaccaaa gtctgggacg gcaaacagga
     8341 tatttatcac ctgctgcatt gcctggtgat cccgcgtatc ccgttagcgc ccggcatggc
     8401 tccagcggtt gcagcgggtc tgctggatat taatgcgaaa taactgatct gaaaataggg
     8461 tgcgggtttt cccctcaccc taaccctctc cccaaagggg cgaggggacc gtccggtgca
     8521 gtttttgggt ttgacatcag cctcacggga ggctgtatgt cgttaattaa ggtgccttgt
     8581 atgcaaatca ccaatatgca ttgcagtgga cagaccgttt cacttgccgc gggggattat
     8641 cacgcgacca ttgtgaccgt cggtgctggt ctggctgaat taacctttca gggttgtcat
     8701 ttagtcatcc cgcacaaacc cgaagagatg ccgctggcgc atctgggtaa agtgcttatt
     8761 ccgtggccga accggatcgc caacggctgt taccgttatc aggggcagga atatcagtta
     8821 cccattaatg aacatagttc aaaagccgct attcacgggc tgcttgcctg gcgagactgg
     8881 caaattagtg aattaaccac caccagcgtt accctgacag cttttctgcc gcccagttac
     8941 ggctatccgt ttatgctggc ttcacaagtt gtttattcgc tcaacgcaca cacgggtctt
     9001 tccgttgaga tcgcaagcca gaatatcggc actgtcgcag cgccttatgg cgtgggtatt
     9061 catccttatt taacctgcaa cttaacatct gttgatgaat atctgttcca gcttccggca
     9121 aaccaggttt atgccgtcga tgaacacgta aatccgacaa cgcttcacca cgttgatgag
     9181 ctggatttaa atttcacgca ggcgaaaaaa atagccgcga caaaaatcga ccacaccttt
     9241 aaaaccgcta atgatttgtg ggaaatgacc attacccatc cacagcaggc gctttcggta
     9301 agcctttgtt ccgatcaacc ctgggtgcag gtttatagcg gtgaaaaatt acagcgtcag
     9361 ggactggcag ttgaacctat gagttgcccg cctaacgcct ttaattcagg tatcgactta
     9421 ttattactgg aaccaggaaa accgcaccgc ctgtttttta atatttacgg acagagaaaa
     9481 taacttgtat tgattagcag tcaaaatcat tgaagaacat ttcactcgct aaaggcaaca
     9541 ttatgtatct tcaatttatt aagttaaaaa cagctcagct tctgtaaagt ttttatatat
     9601 cagttttatg agaatcattt tacataagcc tgtaataaca ggaaggcaac aatctcgttc
     9661 tcaactctat ggagattagt tatggatacg ccacgtccac agttattaga ttttcaattt
     9721 catcagaata acgacagttt taccctacat tttcaacaac gtcttatttt aacccataac
     9781 aaagataatc cttgtttatg gattggctca ggtatagcgg atatcgatat gttccgcggt
     9841 aatttcagca ttaaagataa actacaggag aaaattgcgc ttaccgacgc catcgtcagc
     9901 cagtcaccgg atggttggtt aattcatttc agccgtggtt ctgacattag cgccacgctg
     9961 aatatctctg ccgacgatca gggccgttta ttgctggaac tacaaaacga caaccttaac
    10021 cacaaccgta tctggctgcg ccttgccgct caaccagagg accatatcta cggctgcggc
    10081 gaacagtttt cctacttcga tctgcgcggc aaaccgttcc cgctatggac cagtgaacaa
    10141 ggcgttggtc gcaacaaaca aacctatgtc acctggcagg ccgactgcaa agaaaatgcg
    10201 ggcggcgact attactggac tttcttccca cagcctacgt ttgtcagcac gcagaagtat
    10261 tactgccatg ttgataacag ttgctacatg aacttcgact ttagcgcccc ggaataccat
    10321 gaactggcgc tgtgggaaga caaagctacg ctacgttttg aatgtgctga cacatacatc
    10381 tccctgctgg aaaaattaac tgccctgctg ggacgccagc cagaactgcc cgactggatt
    10441 tatgacgggg taacgctcgg cattcagggc gggacggaag tgtgccagaa gaaactggac
    10501 accatgcgta acgcgggcgt gaaggttaac ggcatctggg cgcaggactg gtctgggatc
    10561 cgtataacct cttttggcaa gcgcgtgatg tggaactgga agtggaacag cgaaaactac
    10621 ccgcaactgg attcacgcat taagcagtgg tatcaggagg gcgtgcagtt cctggcctat
    10681 atcaacccgt atgttgccag cgataaagat ctctgcgaag aagcggcaca acacggctat
    10741 ctagcaaaag atgcctctgg cggtgactat ctggtggagt ttggcgagtt ttacggcggc
    10801 gttgtcgatc tcactaatcc agaagcctac gcctggttca aggaagtgat caaaaagaac
    10861 atgattgaac tcggctgcgg cggctggatg gctgacttcg gcgagtatct gcccaccgac
    10921 acgtacctgc ataacggcgt cagtgccgaa attatgcata acgcctggcc tgcgctgtgg
    10981 gcgaagtgta actacgaagc ccttgaagaa acgggcaagc tcggcgagat cctcttcttt
    11041 atgcgcgccg gttctaccgg tagccagaaa tactccacca tgatgtgggc gggcgaccag
    11101 aacgtggact ggagtctcga cgatggcctg gcgtccgtag tcccggcggc gctgtcgctg
    11161 gcaatgaccg gacatggtct gcatcacagc gacattggcg gttacaccac cctgtttgag
    11221 atgaagcgca gcaaagagct gctgctgcgc tggtgcgatt tcagcgcctt cacgccgatg
    11281 atgcgcaccc acgaaggtaa ccgtcctggc gacaactggc agtttgacgg cgatgcggaa
    11341 actatcgccc attttgcccg tatgactacc gttttcacca ccctgaaacc ttatctgaaa
    11401 gaggccgtcg cgctgaatgc gaagtccggt ctgccggtca tgcgcccgct gttcctgcat
    11461 tacgaagacg atgcacatac ctattccctg aaatatcagt acctgttagg ccgcgacatt
    11521 ctggtcgctc cggtacacga agaaggtcgc agcgactgga cgctctatct gccggaggat
    11581 aactgggtac atgcctggac gggtgaagcg ttccggggcg gggaagttac cgttaatgcg
    11641 cccatcggca agccgccggt cttttatcgc gccgatagcg aatgggcggc actgttcgcg
    11701 tcgttaaaca gcatctaatc acatccgccc gtggggaaac tcatgggcaa agggagaaca
    11761 tcgatgagtc acatcacaac ggaagatcca gcaactttac gcctgccctt taaagagaaa
    11821 ctctcttacg gtattggcga cctggcctct aacatcctgc tggatatcgg tacgctttat
    11881 cttttgaagt tttataccga cgttctgggg ctgccaggca cctatggcgg cattatcttt
    11941 ttgatttcaa aattctttac tgcgtttacc gatatgggta ccggcattat gttggattcc
    12001 cgacgcaaga tcggtccaaa aggtaagttc cgtcctttta ttctgtatgc gtcattcccg
    12061 gtcaccttac tggcgatcgc caactttgtc ggcacaccgt ttgatgtcac cggtaaaacg
    12121 gtgatggcca ctattctgtt tatgctttac ggactgtttt tcagcatgat gaactgctcc
    12181 tacggcgcaa tggtcccggc aatcaccaaa aaccccaacg aacgcgcctc actggcggca
    12241 tggcgtcagg gcggcgctac gctgggcctg ctgctgtgca cggtgggatt cgtgccagtt
    12301 atgaatctta tcgaaggtaa tcagaaactt ggctatatct tcgccgccac gctgttttca
    12361 ctgttcggcc tgctgtttat gtggatctgc tactcaggcg tgaaagagcg ttatgtcgaa
    12421 acccaaccaa ccaatccggc gcaaaagcct ggcctgttgc aatctttccg cgcaattgcc
    12481 ggtaaccgcc cactgttcat tctgtgtatt gccaacctct gcaccttagg ggcgtttaac
    12541 gtcaagctcg ccattcaggt ctattacacc cagtacgtac ttaacgatcc catcctgttg
    12601 tcgtatatgg gatttttcag catgggctgt attttcatcg gcgtgttcct gatgcctggc
    12661 gcagtcagac gttttggtaa gaagaaggtc tatatcggcg gcctgctgat ttgggtgctg
    12721 ggcgatctgc tcaactattt cttcggcggc ggctcggtca gctttgtggc gttctcctgc
    12781 ctggcgttct tcggctcagc gcctgaactg ggcgctggtt tccgacaccg tcgagtacgg
    12841 cgagtggcgc accggcgtgc gttcggaagg cacggtctac accggcttca ccttctttcg
    12901 caaagtgtct caggcgctgg ctggtttctt ccccggctgg atgctgacgc aaattggcta
    12961 tgtgccgaac gtcgcacagg ctgaccacac cattgaaggg ttacgccagt tgatcttcat
    13021 ctacccaagc gcactggcgg tagtcaccat tgtggcgatg ggttgcttct acagcctgaa
    13081 cgagaagatg tatgtccgca ttgtggaaga gatagaagcc cgtaaacgca cggcgtaata
    13141 ataataaacg acgccctgcg gggcgttata aggagtgatt atgtctgacc ataatccact
    13201 gacattaaaa ctgaatctgc gggaaaaaat cgcctatggt atgggcgacg tcggttcgaa
    13261 tttaatgctc tgcatcggta ctctgtatct cctcaaactt tataccgatg aattagggat
    13321 gcctgcttac tatggcggga ttatctttct ggtcgcgaag ttttttaccg cgtttaccga
    13381 tatgctcacc ggatttttac tcgactcgcg gaaaaatatt gggcctaaag gcaaatttcg
    13441 tccctttatt ttatatgcag cagttccggc ggcgttaatt gcaacgctcc agtttatcgc
    13501 caccaccttt tgtttgccgg ttaaaacgac gattgccacc acgctgttta tgatgtttgg
    13561 actttcatac agtctgatga actgctcgta tggtgcgatg atcccggcaa ttaccaaaaa
    13621 cccgaatgag cgcgcgcagc tcgcggctta ccgccagggt ggtgcaacca tagggctatt
    13681 gatttgtacc gtggcgttta ttccgttgca gtcgcttttt tctgactcaa ccgtcggtta
    13741 tgcctgtgcg gcacttatgt tctccattgg cggctttatt tttatgatgc tgtgctacag
    13801 aggcgtcaaa gagcattatg tggacacagc gccaaccgga cataaagcca gtattctcaa
    13861 atctttttgc gcgatatttc gtaatccacc attgctggtt ttatgcattg ctaacctgtg
    13921 tactctggcg gcatttaata tcaaactggc gattcaggtc tattacaccc agtatgtgct
    13981 gaatgatatt aatttattgt cgtggatggg attcttcagt atgggatgca tcctcgtcgg
    14041 cgtattactg gtgccagtaa ctgtaaaatg ttttggtaaa aaacaggttt atttagctgg
    14101 catggtgcta tgggcggtgg gtgatatact gaattatttc tggggaagta actctttcac
    14161 tttcgtcatg ttctcttgtg tcgccttttt tggcacggcc tttgtaaaca gcctgaactg
    14221 ggcactggta ccagataccg tagattacgg tgaatggaaa accggtattc gtgccgaagg
    14281 ttctgtttat accggttata ccttctttcg taaaatttct gccgcacttg ctggcttctt
    14341 gccaggcatt atgctgacgc aaattggtta tgttcccaac atcgcgcaaa gcgatgcgac
    14401 attacagggt ttgcgtcagc tcatttttat ctggccttgt gcactggcaa ttattgctgc
    14461 attgacgatg ggattctttt acacactcaa tgaaaaacgg tttgcattga ttattgagga
    14521 aatcaaccaa cgcaaaaata aagagatggc gacagaaaaa aaaacggctt ccgtaacgtt
    14581 ataaatgtca atagccgcta tttccatcct ggtgggtggc ggcctcccta cgtttaaaaa
    14641 atggacttat tatgaaaaag attaatgcaa taattttatt atcttctctg acctctgcct
    14701 cggtatttgc tggcgcatac gtcgaaaaca gggaggcata taatcttgcc tccgatcagg
    14761 gcgaggttat gctacgtgtg ggttataact tcgatatggg cgcgggtatt atgttaacca
    14821 atacctacac ctttcagcga gaagatgaac taaaacatgg ctataatgaa attgaaggtt
    14881 ggtatccttt attcaaacca accgataaat taaccatcca gcccggtggc ttaattaatg
    14941 ataagagtat cagttcgggt ggtgcagtgt atctggac
//
LOCUS       DAZXUL010000103        14644 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZXUL010000103 DAZXUL010000000
VERSION     DAZXUL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14644
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.103"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACH3L1_003171"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACH3L1_003171"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFT4077817.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACH3L1_003172"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACH3L1_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFT4077818.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACH3L1_003173"
     CDS             complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACH3L1_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFT4077819.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACH3L1_003174"
     CDS             complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACH3L1_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFT4077820.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="ACH3L1_003175"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="ACH3L1_003175"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFT4077821.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="ACH3L1_003176"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="ACH3L1_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFT4077822.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="ACH3L1_003177"
     CDS             5920..6417
                     /locus_tag="ACH3L1_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFT4077823.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="ACH3L1_003178"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="ACH3L1_003178"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFT4077824.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="ACH3L1_003179"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="ACH3L1_003179"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFT4077825.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="ACH3L1_003180"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="ACH3L1_003180"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFT4077826.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="ACH3L1_003181"
     CDS             9102..9665
                     /locus_tag="ACH3L1_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFT4077827.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="ACH3L1_003182"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="ACH3L1_003182"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFT4077828.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="ACH3L1_003183"
     CDS             complement(10257..11237)
                     /locus_tag="ACH3L1_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFT4077829.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="ACH3L1_003184"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="ACH3L1_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFT4077830.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="ACH3L1_003185"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="ACH3L1_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFT4077831.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /gene="yggU"
                     /locus_tag="ACH3L1_003186"
     CDS             12540..12830
                     /gene="yggU"
                     /locus_tag="ACH3L1_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFT4077832.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="ACH3L1_003187"
     CDS             12838..13431
                     /locus_tag="ACH3L1_003187"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFT4077833.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="ACH3L1_003188"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="ACH3L1_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFT4077834.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         3532 a         3873 c         3757 g         3482 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaat
//
LOCUS       DAZXUL010000104        14318 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZXUL010000104 DAZXUL010000000
VERSION     DAZXUL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14318
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.104"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..3051
                     /locus_tag="ACH3L1_003189"
                     /pseudo
     CDS             1..3051
                     /locus_tag="ACH3L1_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="ACH3L1_003190"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="ACH3L1_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="ACH3L1_003191"
     CDS             6768..7175
                     /locus_tag="ACH3L1_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077835.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="ACH3L1_003192"
     CDS             7717..8805
                     /locus_tag="ACH3L1_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFT4077836.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="ACH3L1_003193"
     CDS             8816..11095
                     /locus_tag="ACH3L1_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFT4077837.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12224
                     /locus_tag="ACH3L1_003194"
     CDS             11088..12224
                     /locus_tag="ACH3L1_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFT4077838.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12221..14224
                     /locus_tag="ACH3L1_003195"
     CDS             12221..14224
                     /locus_tag="ACH3L1_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077839.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3547 a         3755 c         3655 g         3361 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggtcc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg acacaagcgt cgttgatctc acggcagacg
    11881 ttgccgatcc ggtagagtta ttaatgaaag tgcaattagg cggcggaacc aatatcgcca
    11941 gtgccatgga gtatggtcgg caacttattg aacaacctgc aaaaagcgtc attatcctcg
    12001 tgagcgattt ttatgaaggg ggttcatcat cattgctgac gcaccaagtg aaaaagtgtg
    12061 tccagagcgg catcaaagtg ctgggactgg cagcgctcga tagcacagca acaccttgct
    12121 atgaccgcga tacggcccag gcgctggtta atgtcggcgc acaaatagcc gccatgacac
    12181 cgggcgagct ggcatcatgg cttgcggaga atcttcagtc atgaattcac tacgtccgga
    12241 attattagaa ctgacaccgc aggcactgac ggcgttaagc aatgccggtt ttgttaagcg
    12301 cagccttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga acagcgcttt
    12361 aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacggccagg cactgaaaga
    12421 ggctcagtgc acctatgggg ccagcggcat gtgccgtcat cgcgtgatgc tggtgttaag
    12481 ttatcaacga ctttgtgcta ccgcccagcc tacgggaaaa gaagaggaat gggatccggc
    12541 aatctggctg gaagaactgg ctacccttcc cgatgccacc cgcaaacgcg cacaggcgct
    12601 ggtcgctaaa agcatcacca ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg
    12661 cttaccgatg agcgatgtgc gtttttattc ccgcagcagt attcgtttcg cccgttgtga
    12721 ttgtattgaa ggcacacttt gcgaacatgt cgtactggca gtacaggcct ttgtccaggc
    12781 aaaagcgcag caagcggaat tgactcattt aatctggcag atgcgcagcg aacacgtcac
    12841 atcatctgac gatccgtttg ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca
    12901 actaagccag gcattatggc tgagcggcat cagccagccg cttattcact acgaggccgc
    12961 tttcagccgc gcgcagcaag cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct
    13021 acggcaactg cgcgccagcg ttgatgcctt ccacgcccgc gccagccact atcatgccgg
    13081 agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc
    13141 ccggagcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg
    13201 cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg
    13261 gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat
    13321 tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa gactcaccgg cagctacgcg
    13381 tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc
    13441 aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt
    13501 accgctgtcg cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt
    13561 ggctttgcgg gaatatttac gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt
    13621 cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg
    13681 ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctgc tgacgttatc
    13741 actgccagcc tcagccagcg cgccttatgc cattgaacgc atggcggcgc ttttgcaaca
    13801 aacagacgac cccgtgtgtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac
    13861 actggaacca caggtaatga tgacaaagac tcgtgcctgg gcgctggacg cagaaactgc
    13921 gcctgtggtg gtatcactac cttctgccag cgttctgcct gtgccgtcta ctgctcatca
    13981 gttgctgatg cgctgccagg cgttacttat tcaactgctc cataacggct ggcgctatca
    14041 ggaacagagc gctattaatc aggcagagtt gctggcgaat gacctcaccg cggtgggttt
    14101 ttatcggctg gcacatgtgt tggcacaatt tcgtaataca gaaagcgagg cacgggtaga
    14161 agcaatgaat aacggtgttt tgctttgcga acaattattc cccatgcttc agcaacaagg
    14221 atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta
    14281 ttaggcatat cctatatagc ccctggtgag agtcacca
//
LOCUS       DAZXUL010000105        13964 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZXUL010000105 DAZXUL010000000
VERSION     DAZXUL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.105"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACH3L1_003196"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACH3L1_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFT4077840.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNIYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACH3L1_003197"
     CDS             2219..2767
                     /locus_tag="ACH3L1_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFT4077841.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACH3L1_003198"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACH3L1_003198"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFT4077842.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACH3L1_003199"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACH3L1_003199"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFT4077843.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACH3L1_003200"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACH3L1_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFT4077844.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="ACH3L1_003201"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="ACH3L1_003201"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFT4077845.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="ACH3L1_003202"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="ACH3L1_003202"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFT4077846.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="ACH3L1_003203"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="ACH3L1_003203"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFT4077847.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="ACH3L1_003204"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="ACH3L1_003204"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFT4077848.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="ACH3L1_003205"
     CDS             complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="ACH3L1_003205"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFT4077849.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3551 a         3562 c         3527 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaata tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc ataggaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagagta cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DAZXUL010000106        13667 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZXUL010000106 DAZXUL010000000
VERSION     DAZXUL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13667
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.106"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..589
                     /locus_tag="ACH3L1_003206"
     CDS             <1..589
                     /locus_tag="ACH3L1_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFT4077850.1"
                     /translation="IREQFPLLPSDTRQIAIDSGIKHPVIRGVDQVMSTDFLVDCKDG
                     PFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPWFIFTDKEINPVVKENIEWLY
                     SVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVDIAYDLELGKTLSEIRALTAN
                     GFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            576..2684
                     /locus_tag="ACH3L1_003207"
     CDS             576..2684
                     /locus_tag="ACH3L1_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFT4077851.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            2681..4336
                     /locus_tag="ACH3L1_003208"
     CDS             2681..4336
                     /locus_tag="ACH3L1_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFT4077852.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     NGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHRELNV
                     EQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMSQIAN
                     AHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFEADLR
                     SARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRDVWYE
                     LSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSGIPER
                     IARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKEDYDSS
                     LLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVVKPSD
                     WDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            4339..5865
                     /locus_tag="ACH3L1_003209"
     CDS             4339..5865
                     /locus_tag="ACH3L1_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFT4077853.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAIRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            5866..7482
                     /locus_tag="ACH3L1_003210"
     CDS             5866..7482
                     /locus_tag="ACH3L1_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFT4077854.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(7538..7690)
                     /locus_tag="ACH3L1_003211"
                     /pseudo
     CDS             complement(7538..7690)
                     /locus_tag="ACH3L1_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(7713..8090)
                     /locus_tag="ACH3L1_003212"
     CDS             complement(7713..8090)
                     /locus_tag="ACH3L1_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077855.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(8500..8871)
                     /locus_tag="ACH3L1_003213"
     CDS             complement(8500..8871)
                     /locus_tag="ACH3L1_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077856.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(8932..9429)
                     /locus_tag="ACH3L1_003214"
     CDS             complement(8932..9429)
                     /locus_tag="ACH3L1_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077857.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(9458..10293)
                     /gene="aadA1"
                     /locus_tag="ACH3L1_003215"
                     /pseudo
     CDS             complement(9458..10293)
                     /gene="aadA1"
                     /locus_tag="ACH3L1_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            complement(10351..10875)
                     /gene="sat2"
                     /locus_tag="ACH3L1_003216"
     CDS             complement(10351..10875)
                     /gene="sat2"
                     /locus_tag="ACH3L1_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFT4077858.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(10970..11443)
                     /gene="dfrA1"
                     /locus_tag="ACH3L1_003217"
     CDS             complement(10970..11443)
                     /gene="dfrA1"
                     /locus_tag="ACH3L1_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFT4077859.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            11775..12752
                     /gene="intI2"
                     /locus_tag="ACH3L1_003218"
                     /pseudo
     CDS             11775..12752
                     /gene="intI2"
                     /locus_tag="ACH3L1_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(12940..13179)
                     /locus_tag="ACH3L1_003219"
     CDS             complement(12940..13179)
                     /locus_tag="ACH3L1_003219"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFT4077860.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         3783 a         2869 c         3241 g         3774 t
ORIGIN      
        1 tatacgcgag cagttcccct tattacctag tgataccagg cagattgcaa tagatagtgg
       61 tattaagcat cctgttattc gtggtgtaga tcaggttatg tctactgatt ttttagtgga
      121 ctgcaaagat ggtccttttg agcagtttgc tattcaagtc aaacctgcag cagccttaca
      181 agacgagcgt accttagaaa aactagaact agagcgtcgc tattggcagc aaaagcaaat
      241 tccttggttc atttttactg ataaagaaat aaatcccgta gtaaaagaaa atattgaatg
      301 gctttattca gtgaaaacag aagaagtttc tgcggagctt ttagcacaac tatccccatt
      361 ggcccatatc ctgcaagaaa aaggagatga aaacattatc aatgtctgta agcaggttga
      421 tattgcttat gatttggagt taggcaaaac attgagtgag atacgagcct taaccgcaaa
      481 tggttttatt aagttcaata tttataagtc tttcagggca aataagtgtg cagatctctg
      541 tattagccaa gtagtgaata tggaggagtt gcgctatgtg gcaaattaat gaggttgtgc
      601 tatttgataa tgatccgtat cgcattttgg ctatagagga tggccaagtt gtctggatgc
      661 aaataagcgc tgataaagga gttccacaag ctagggctga gttgttgcta atgcagtatt
      721 tagatgaagg ccgcttagtt agaactgatg acccttatgt acatcttgat ttagaagagc
      781 cgtctgtaga ttctgtcagc ttccagaagc gcgaggagga ttatcgaaaa attcttccta
      841 ttattaatag taaggatcgt ttcgacccta aagtcagaag cgaactcgtt gagcatgtgg
      901 tccaagaaca taaggttact aaggctacag tttataagtt gttacgccgt tactggcagc
      961 gtggtcaaac gcctaatgca ttaattcctg actacaaaaa cagcggtgca ccaggggaaa
     1021 gacgttcagc gacaggaaca gcaaagattg gccgagccag agaatatggt aagggtgaag
     1081 gaaccaaggt aacgcccgag attgaacgcc tttttaggtt gaccatagaa aagcacctgt
     1141 taaatcaaaa aggtacaaag accaccgttg cctatagacg atttgtggac ttgtttgctc
     1201 agtattttcc tcgcattccc caagaggatt acccaacact acgtcagttt cgttattttt
     1261 atgatcgaga ataccctaaa gctcagcgct taaagtctag agttaaagca ggggtatata
     1321 aaaaagacgt acgaccctta agtagtacag ccacttctca ggcgttaggc cctgggagtc
     1381 gttatgagat tgatgccacg attgctgata tttatttagt ggatcatcat gatcgccaaa
     1441 aaatcatagg aagaccaacg ctttacattg tgattgatgt gtttagtcgg atgatcacgg
     1501 gcttttatat cggctttgaa aatccgtctt atgtggtggc gatgcaggct tttgtaaatg
     1561 cttgctctga caaaacggcc atttgtgccc agcatgatat tgagattagt agctcagact
     1621 ggccgtgtgt aggtttgcca gatgtgttgc tagcggaccg tggcgaatta atgagtcatc
     1681 aggtcgaagc cttagtttct agttttaatg tgcgagtgga aagtgctcca cctagacgtg
     1741 gcgatgctaa aggcatagtg gaaagcactt ttagaacact acaagccgag tttaagtcct
     1801 ttgcacctgg cattgtagag ggcagtcgga tcaaaagcca tggtgaaaca gactataggt
     1861 tagatgcatc tctgtcggta tttgagttca cacaaattat tttgcgtacg atcttattca
     1921 gaaataacca tctggtgatg gataaatacg atcgagatgc tgattttcct acagatttac
     1981 cgtctattcc tgtccagcta tggcaatggg gtatgcagca tcgtacaggt agtttaaggg
     2041 ctgtggagca agagcagttg cgagtagcgt tactgcctcg ccgaaaggtc tctatttctt
     2101 catttggcgt taatttgtgg ggtttgtatt actcggggtc agagattctg cgtgagggtt
     2161 ggttgcagcg gagcactgat atagctagac ctcaacattt agaagcggct tatgacccag
     2221 tgctggttga tacgatttat ttgtttccgc aagttggcag ccgtgtattt tggcgctgta
     2281 atctgacgga acgtagtcgg cagtttaaag gtctctcatt ttgggaggtt tgggatatac
     2341 aagcacaaga aaaacacaat aaagccaatg cgaagcagga tgagttaact aaacgcaggg
     2401 agcttgaggc gtttattcag caaaccattc agaaagcgaa taagttaacg cccagtacta
     2461 ctgagcccaa atcaacacgc attaagcaga ttaaaactaa taaaaaagaa gccgtgacct
     2521 cggagcgtaa aaaacgtgcg gagcatttga agccaagctc ttcaggtgat gaggctaaag
     2581 ttattccttt caacgcagtg gaagcggatg atcaagaaga ttacagccta cccacatacg
     2641 tgcctgaatt atttcaggat ccaccagaaa aggatgagtc atgagtgcta cccggattca
     2701 agcagtttat cgtgatacgg gggtagaggc ttatcgtgat aatcctttta tcgaggcctt
     2761 accaccatta caagagtcag tgaatagtgc tgcatcactg aaatcctctt tacagcttac
     2821 ttcctctgac ttgcaaaagt cccgtgttat cagagctcat accatttgtc gtattccaga
     2881 tgactatttt cagccattag gtacgcattt gctactaagt gagcgtattt cggtcatgat
     2941 tcgaggtggc tacgtaggca gaaatcctaa aacaggagat ttacaaaatg gttatgagcg
     3001 tgttcaaacg ggagagttgg agacatttcg ctttgaggag gcacgatcta cggcacaaag
     3061 cttattgtta attggttgtt ctggtagtgg gaagacgacc tctcttcatc gtattctagc
     3121 cacgtatcct caggtgattt accatcgtga actcaatgta gagcaggtgg tgtatttgaa
     3181 aatagactgc tcgcataatg gttcgctaaa agaaatctgc ttgaattttt tcagagcgtt
     3241 ggatcgagcc ttgggctcga actatgagcg tcgttatggc ttaaaacgtc atggtataga
     3301 aaccatgttg gctttgatgt cgcaaatagc caatgcacat gctttagggt tgttggttat
     3361 tgatgaaatt cagcatttaa gccgctctcg ttcgggtgga tctcaagaga tgctgaactt
     3421 ttttgtgacg atggtgaata ttattggcgt accagtgatg ttgattggta cccctaaagc
     3481 acgagagatt tttgaggctg atttgcggtc tgcacgtaga ggggcagggt ttggagctat
     3541 attctgggat cctatacaac aaacgcaacg tggaaagccc aatcaagagt ggatcgcttt
     3601 tacggataat ctttggcaat tacagctttt acaacgcaaa gatgcgctgt tatcggatga
     3661 ggtccgtgat gtgtggtatg agctaagcca aggagtgatg gacattgtag taaaactttt
     3721 tgtactcgct cagctccgtg cgctagcttt aggcaatgag cgtattaccg ctggtttatt
     3781 gcggcaagtg tatcaagatg agttaaagcc tgtgcacccc atgctagagg cattacgctc
     3841 gggtatccca gaacgcattg ctcgttattc tgatctagtc gttcccgaga ttgataaacg
     3901 gttaatccaa cttcagctag atatcgcagc gatacaagaa caaacaccag aagaaaaagc
     3961 ccttcaagag ttagataccg aagatcagcg tcatttatat ctgatgctga aagaggatta
     4021 cgattcaagc ctgttaattc ccactattaa aaaagcgttt agccagaatc caacgatgac
     4081 aagacaaaag ttactgcctc ttgttttgca gtggttgatg gaaggcgaaa cggtagtgtc
     4141 agaactagaa aagccctcca agagtaaaaa ggtttcggct ataaaggtag tcaagcccag
     4201 cgactgggat agcttgcctg atacggattt acgttatatc tattcacaac gccaacctga
     4261 aaaaaccatg catgaacggt taaaagggaa aggggtaata gtggatatgg cgagcttatt
     4321 taaacaagca ggttagccat gagaaacttt cctgttccgt actcgaatga gctgatttat
     4381 agcactattg cacgggcagg cgtttatcaa gggattgtta gtcctaagca gctgttggat
     4441 gaggtgtatg gcaaccgcaa ggtggtcgct accttaggtc tgccctcgca tttaggtgtg
     4501 atagcaagac atctacatca aacaggacgt tacgctgttc agcagcttat ttatgagcat
     4561 accttattcc ctttatatgc tccgtttgta ggcaaggagc gccgagacga agctattcgg
     4621 ttaatggagt accaagcgca aggtgcggtg catttaatgc taggagtcgc tgcttctaga
     4681 gttaagagcg ataaccgctt tagatactgc cctgattgcg ttgctcttca gctaaatagg
     4741 tatggggaag ccttttggca acgagattgg tatttgcccg ctttgccata ttgtccaaaa
     4801 cacggtgctt tagtcttctt tgatagagct gtagatgatc accgacatca attttgggct
     4861 ttgggtcata ctgagctgct ttcagactac cccaaagact ccctatctca attaacagca
     4921 ctagctgctt atatagcccc tctgttagat gctccacgag cgcaagagct ttccccaagc
     4981 cttgagcagt ggacgctgtt ttatcagcgc ttagcgcagg atctagggct aaccaaaagc
     5041 aagcacattc gtcatgactt ggtggcggag agagtgaggc agacttttag tgatgaggca
     5101 ctagagaaac tggatttaaa gttggcagag aacaaggaca cgtgttggct gaaaagtata
     5161 ttccgtaagc atagaaaagc ctttagttat ttacagcata gtattgtgtg gcaagcctta
     5221 ttgccaaaac taacggttat agaagcgcta cagcaggcaa gtgctcttac tgagcactct
     5281 ataacgacaa gacctgttag ccagtctgtg caacctaact ctgaagattt atctgttaag
     5341 cataaagact ggcagcaact agtgcataaa taccaaggaa ttaaggcggc aagacagtct
     5401 ttagagggtg gggtgctata cgcttggctt taccgacatg acagggattg gctagttcac
     5461 tggaatcaac agcatcaaca agagcgtctg gcacccgccc ctagagttga ttggaaccaa
     5521 agagatcgaa ttgctatacg acaactatta agaatcataa agcgtctaga tagtagcctt
     5581 gatcacccaa gagcgacatc gagctggctg ttaaagcaaa ctcctaacgg aacctctctt
     5641 gcaaaaaatc tacagaaact gcctttggta gcgctttgct taaagcgtta ctcagagagt
     5701 gtggaagatt atcaaattag acggattagc caagctttta ttaagcttaa acaggaagat
     5761 gttgagctta ggcgctggcg attattaaga agtgcaacgt tatctaaaga gcggataact
     5821 gaggaagcac aaagattctt ggaaatggtt tatggggaag agtgagtggt taggctagct
     5881 acatttaatg acaatgtgca ggttgtacat attggtcatt tattccgtaa ctcgggtcat
     5941 aaggagtggc gtatttttgt ttggtttaat ccaatgcaag aacggaaatg gactcgattt
     6001 actcatttgc ctttattaag tcgagctaag gtggttaaca gtacaacaaa gcaaataaat
     6061 aaggcggatc gtgtgattga gtttgaagca tcggatcttc aacgagccaa aataatcgat
     6121 tttcctaatc tctcgtcctt tgcttccgta cgcaacaagg atggagcgca gagttcattt
     6181 atttacgaag ctgaaacacc atatagcaag actcgttatc acatcccaca gttagagcta
     6241 gctcggtcat tatttttaat taactcctat ttctgtcgaa gctgtttgag cagtaccgct
     6301 ttacagcaag agttcgacgt tcagtatgag gttgagcgag atcatttaga gataaggatc
     6361 ttacccagtt catcgtttcc taaaggggcg ttagagctgt cggccgtagt gcagcttttg
     6421 gtttggttgt tttcggatca agatgttatg gattcgtatg aaagtatttt taggcactat
     6481 caacaaaata gagaaattaa gaacggcgtt gaaagctggt gctttagctt tgaccctccg
     6541 cccatgcagg gttggaaatt acatgtaaaa ggacgttctt ctaacgagga taaggattat
     6601 ttagttgagg aaatagtagg tttagaaatc aacgctatgc ttcctagcac aacagctatt
     6661 agccatgcct cttttcagga aaaggaggca ggtgatggta gtacgcagca catagcggtt
     6721 tcaacagagt cagttgttga tgatgagcat ctacagttgg acgatgagga aacagccaat
     6781 atagacacag acacacgagt catagaggct gagccgacat ggataagttt tagtagacct
     6841 agtcgaattg aaaaatctcg cagggcaaga aaaagtagcc aaactatttt agaaaaagaa
     6901 gaagcaacaa caagtgaaaa tagtaatttg gttagtactg atgagccaca cttaggtggt
     6961 gtcctagcag cggcagatgt gggtgggaag caggatgcaa ccaattacaa ctctattttt
     7021 gctaatcgat ttgctgcttt tgatgagcta ctttcaattc taaaaactaa atttgcatgt
     7081 cgggtgcttt ttgaagaaac cttggttttg ccaaaagttg ggcgtagccg attacatctg
     7141 tgtaaagatg gctcaccaag agtgattaaa gccgttgggg tgcaacgtaa tggcagtgaa
     7201 tttgtattgc tagaggtgga tgcatcggat ggggtgaaaa tgctttctac caaagtgttg
     7261 agtggcgttg atagcgaaac atggcggaat gattttgaaa agatacggcg tggagtggtg
     7321 aagagctcat tgaattggcc aaatagtttg tttgatcaat tatatggaca agacgggcat
     7381 agaggggtga atcatccaaa ggggttgggg gagctgcaag tatcgagaga ggatatggaa
     7441 gggtgggctg agagagtggt tagagagcaa tttacgcatt aaaggaatga ctgaaagagc
     7501 ctgtaaaccc ttttgtgtaa gtgcttttgc cggtcagtta aaggtggcca tttaaacggt
     7561 caccaaattc gatcataaaa cggttcatgg ccggcttcca gttgcggatc ggcatcgtcc
     7621 atttcttggt cgccgcctgg atagccaggt acaccacctt catcgctgat tcgtccgtag
     7681 ggaacacctt gagttcatat taatggaatt ttctacaaat agcctccgtg gttttgaggg
     7741 gggattacag acgatccata gtagtaatcc aatgagttct tgagcgcggc gacatgtttg
     7801 gacgccttgg caaaaattag agcctgcttg aagtgcaggc gagcacgtgc ttggacgatt
     7861 gatccattcg cggtcaaaaa ctcaatcttg gatgacagcg tgtcaggaaa tccaatatcg
     7921 aagtcccgcc ccgtgtcaaa gtagggcatt tcatgatcaa aggacgaagc ttcccaggcc
     7981 acttccctaa tattccagcc gctagaaaca agatttcgta cgacttcatc aacttttttg
     8041 ctttccaact tgtcacaaag tgcataggca agagctttgg cggcagacac gtgcgtttcc
     8101 tcttaaacat aggtcgaacg gatacccgag gcaaagcgga gcattagtcc caaagcatct
     8161 ccggtagatc cggaggctcc atactgaagg catggcatct tgggcattga tatttgaaac
     8221 ttctctctag atagcaggga tttcttggcc ttgcctcatg ctgatagctt gtaaatatca
     8281 cacgatcctt agccccacaa agtgagcata ctccgtagct ttcatttata gaaatcgtcg
     8341 ttagattgtc gcagcatgtg cacgttccga ctatttgctc aggaagcatc gccatacctt
     8401 ggcctaggta aagaagatca gactcgtagc cacatcggtg gcaactgagc ttgtacgcaa
     8461 tgcccatctg gttgtctcac ctgaatcgta acgtcagagc tattcatcca tccattcggc
     8521 atcaatgtat gccactaagc cgaccagctg accgtttgga ctgaagtatg ttgcctgcac
     8581 cccacttgac atctcatcaa taccaccgca ctgcgctagg cgccggtccc acttgcccac
     8641 agaagcgtca ggatcggctg agttccccaa gacccttgtg agccatgcct cctttaactc
     8701 aacctctcga atgattgcgt cggccagctc ctccgcgtta tctagttcga catagtctgg
     8761 aattcggacg tacgttctga acacagctgg atacttactt gggcgattgt catacatgac
     8821 atcaacaatg tacccgtttg tgtaaccgtc tcttggaggt tcgtatgaca ctagtggttc
     8881 ccctcagctt gcgactagat gttgaggcct aacattttat tagagagcag gctagttgct
     8941 tagatacatg atcttcaggc cgttatctgt cagggcaagc gaaaattggc catttatgac
     9001 gaccaatgcc ccgcagaagc tcccatcttt gccgccatag acgccgcgcc ccccttttgg
     9061 ggtgtagaac atccttttgc cagatgtgga aaagaagttc gttgtcccat tgttggcaat
     9121 gacgtagtag ccggcgaaag tgcgagaccc atttgcgcta tatataagcc tacgatttcc
     9181 gttgcgacta ttgtcgtaat tggatgaact attatcgtag ttgctctcag agttgtcgta
     9241 atttgatgga ctattgtcgt aattgcttat ggagttgtcg tagttgcttg gagaaatgtc
     9301 gtagttggat ggggagtagt catagggaag acgagcttca tccactaaaa caattggcag
     9361 gtcagcaagt gcctgccccg atgccatcgc aagtacgagg cttagaacca ccttcaacag
     9421 atcgcgcata gtcttcccca gctctctaac gcttgagtta agccgcgccg cgaagcggcg
     9481 tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct cgcctttcac
     9541 gtagtgaaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttgtccaag
     9601 ataagcctgc ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc
     9661 ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg
     9721 ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag
     9781 cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc
     9841 ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc
     9901 cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca
     9961 ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac
    10021 aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc
    10081 caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac
    10141 cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg
    10201 tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg
    10261 agtcgatact tcggcgatca ccgcttccct catgatgttt aactcctgaa ttaagccgcg
    10321 ccgcgaagcg gtgtcggctt gaatgaattg ttaggcgtca tcctgtgctc ccgagaacca
    10381 gtaccagtac atcgctgttt cgttcgagac ttgaggtcta gttttatacg tgaacaggtc
    10441 aatgccgccg agagtaaagc cacattttgc gtacaaattg caggcaggta cattgttcgt
    10501 ttgtgtctct aatcgtatgc caaggagctg tctgcttagt gcccactttt tcgcaaattc
    10561 gatgagactg tgcgcgactc ctttgcctcg gtgcgtgtgc gacacaacaa tgtgttcgat
    10621 agaggctaga tcgttccatg ttgagttgag ttcaatcttc ccgacaagct cttggtcgat
    10681 gaatgcgcca tagcaagcag agtcttcatc agagtcatca tccgagatgt aatccttccg
    10741 gtaggggctc acacttctgg tagatagttc aaagccttgg tcggataggt gcacatcgaa
    10801 cacttcacga acaatgaaat ggttctcagc atccaatgtt tccgccacct gctcagggat
    10861 caccgaaatc ttcatatgac gcctaacgcc tggcacagcg gatcgcaaac ctggcgcggc
    10921 ttttggtaca aaaggcgtga caggtttgcg aatccgttgc tgccacttgt taaccctttt
    10981 gccagatttg gtaactataa tttatgttag aggcgaagtc ttgggtaaaa actggcctaa
    11041 aattgctggg gatttcagga aagtaaacat caccttccgg ctcgatgtct attgtagata
    11101 tatgtagtgt atctacttga tcgatcaggc ttttgtatat ctccccacca cctgaaacaa
    11161 tgacatgatc cgttattttc tttaggttgg ttaaagcatc tttaattgat ggaaagatca
    11221 atacgttctc attgtcagat gtaaaacttg aacgtgttac gaccgcatac tttcggttgg
    11281 gtaatgctcc cattgattca aaagtcttgc gtccaaccaa cagccattgg ttataggtaa
    11341 tagctttaaa caggagctgt tcacctttgg cactccatgg aatatcaggg ccattcccga
    11401 taactccatt cttcgatata gctaccatta gtgatagttt cacaattctt cctcagaggt
    11461 taacatttta ttagtaagca tgctcgtttt gtcacccgct gatgcttacc gttaattaat
    11521 ataatcactt gtattaaata gactttcttt aaaaatacaa gacactctgt tattacaaat
    11581 cgtgcatgcc gtctatcctg cttgcacgat gcacattatc tcacccaaag aactcataac
    11641 attttaattt atttagcatt aaattaaaac tctaccggta aacaagcatc tctaggcgta
    11701 aaaaacaggc acttttttaa atcccagcta gaataggctg tataggcaga cagttgcaag
    11761 acaaggagcg ttttatgtct aacagtccat ttttaaattc tatacgcacg gatatgcgac
    11821 aaaaaggtta tgcgctgaaa actgaaaaaa cttacctgca ctggattaag cgttttattc
    11881 tgtttcacaa aaaacgtcat cctcagacca tgggcagtga agaggtcagg ctgtttttat
    11941 ccagcttagc aaacagcaga catgtagcca taaacacgca gaaaatcgct ttaaatgccc
    12001 tagctttttt gtacaacagg tttttacaac agccgttggg cgatattgat tatatccctg
    12061 caagcaagcc tagacggcta ccctctgtta tctctgcaaa tgaagtgcaa cgcattttgc
    12121 aggttatgga tactcgcaac caagttattt ttacgctgct gtatggtgca ggtttgcgca
    12181 ttaatgaatg cttgcgtttg cgggttaaag attttgattt tgataatggc tgcatcactg
    12241 tgcatgacgg taagggtggg aaaagcagaa acagcctact gcccacgcgc ctaatcccag
    12301 caataaaata actcattgag caagcgcggc ttattcagca agacgacaac ttacaaggcg
    12361 tagggccatc gctgcctttt gctttagatc acaaataccc ttctgcttat cgacaagcgg
    12421 cgtggatgtt tgtctttccc tccagcacgc tctgcaacca cccgtataac ggcaaattat
    12481 gccgccatca tctgcatgac tccgttgcgc gaaaggcatt gaaggcagcc gtacaaaaag
    12541 caggcatcgt tagcaagcgt gtcacttgtc atacatttcg tcactcgttt gctacgcatc
    12601 tattacaagc ggggcgtgat attcgcactg tgcaagaact cttagggcat aacgatgtta
    12661 agaccacgca aatctatacg catgtgttgg gtcagcattt tgccggcacc accagtcctg
    12721 cggatggact gatgctactt atcaatcagt aaaagtagat aaatatcttt atgtagccct
    12781 gagtagcccc tgatactttt tatggtgaaa aagataaaca atcatagagg ggataaatat
    12841 gagccagttc taccattgtt cagataagtt taagcaagat actgtcaatc acagtgacca
    12901 ttcatggtca ttacacttcc tccagttgcc agacaatgcc taacataaaa acgcaaaagc
    12961 ccaaaagtca gagcttaggt gttctaggcg ttttctaagc gctgaagaaa tacgtcttac
    13021 cgtagattgc gtgcgtttct gaagcacatt ttccttatac atggctttat cccaggcagc
    13081 ttcatcaagc tcttggagta acaagccagt aataatgcga cactctcggg tttgtagcga
    13141 gccgctggtg agatcggagt tatagcggaa aggggtcatt tgtaattatt tgtgggtgga
    13201 ggatagttaa cttattattg tagttggaag aggctgcttt ccaaacatta gcacctcgta
    13261 atgcttgtta ctgtatatta atttatgctc ttagcgcatg ttaatgggct attacaatgc
    13321 ataatgtcca catcaagata actgtggctt aacacctgta caagcgaaaa aaatcaatca
    13381 cctgtccgga aatacgggat cactgtaaag tgttggtgag gcagtctagg agggggattt
    13441 aatttgggat actgatgaaa ggattatgat cagttggata taggatcgta ctaagctgag
    13501 cggcaggtta gctagattag ttaaccagat aagtgaaatc tagttccaaa ctattttgtc
    13561 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc
    13621 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcc
//
LOCUS       DAZXUL010000107        13248 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZXUL010000107 DAZXUL010000000
VERSION     DAZXUL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.107"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACH3L1_003220"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACH3L1_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACH3L1_003221"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACH3L1_003221"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFT4077861.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="ACH3L1_003222"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="ACH3L1_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACH3L1_003223"
     CDS             complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACH3L1_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFT4077862.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACH3L1_003224"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACH3L1_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFT4077863.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRLGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="ACH3L1_003225"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="ACH3L1_003225"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFT4077864.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="ACH3L1_003226"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="ACH3L1_003226"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFT4077865.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8270..9031
                     /gene="rhaD"
                     /locus_tag="ACH3L1_003227"
     CDS             8270..9031
                     /gene="rhaD"
                     /locus_tag="ACH3L1_003227"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFT4077866.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="ACH3L1_003228"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="ACH3L1_003228"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFT4077867.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9656..10102
                     /locus_tag="ACH3L1_003229"
     CDS             9656..10102
                     /locus_tag="ACH3L1_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFT4077868.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10113..11564
                     /locus_tag="ACH3L1_003230"
     CDS             10113..11564
                     /locus_tag="ACH3L1_003230"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFT4077869.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11554..12624
                     /locus_tag="ACH3L1_003231"
     CDS             11554..12624
                     /locus_tag="ACH3L1_003231"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFT4077870.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12624..>13248
                     /locus_tag="ACH3L1_003232"
     CDS             12624..>13248
                     /locus_tag="ACH3L1_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFT4077871.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3158 a         3458 c         3492 g         3140 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtcg tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 aggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat
     9481 gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
     9541 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
     9601 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
     9661 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
     9721 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
     9781 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgcagt
     9841 gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc gcaaagcgca
     9901 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
     9961 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
    10021 tcaccaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc tggccttgtt
    10081 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
    10141 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    10201 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
    10261 accgcttatc cagcgaagag attgccgctg ccgattacgt cattcttgct accgggcgtg
    10321 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
    10381 ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
    10441 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
    10501 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
    10561 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    10621 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
    10681 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    10741 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
    10801 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
    10861 tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
    10921 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
    10981 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
    11041 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
    11101 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
    11161 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
    11221 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
    11281 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
    11341 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
    11401 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg
    11461 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
    11521 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
    11581 tgcgaagcca gcgccgttag cggcgatgaa caggaagttc gcgacatcct gataaacacg
    11641 ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    11701 ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
    11761 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
    11821 tcgatgctca accatcgggt aaccatacgc acacacaagg gagtgaaaat ccctggtgtg
    11881 attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt
    11941 gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg
    12001 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    12061 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
    12121 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
    12181 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt gctggatacc
    12241 gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    12301 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
    12361 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccgga
    12421 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
    12481 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
    12541 ctgctcacgt tgatacgggg ttttctgacg accttaaccg cggagaaagt caacgcgttt
    12601 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    12661 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
    12721 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
    12781 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    12841 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat actttcactc
    12901 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc
    12961 cccagttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct ggtttgggcc
    13021 atttcattga tgagacagaa gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag
    13081 atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat ttatccacgg
    13141 cctgcgacaa ccaacaccgc tggccgctca tacagggtga ttatctctcc agcctgattc
    13201 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaat
//
LOCUS       DAZXUL010000108        13237 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZXUL010000108 DAZXUL010000000
VERSION     DAZXUL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13237
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.108"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1177)
                     /locus_tag="ACH3L1_003233"
     CDS             complement(<1..1177)
                     /locus_tag="ACH3L1_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4077872.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="ACH3L1_003234"
     tRNA            complement(1376..1460)
                     /locus_tag="ACH3L1_003234"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="ACH3L1_003235"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="ACH3L1_003235"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFT4077873.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="ACH3L1_003236"
     CDS             2804..4306
                     /locus_tag="ACH3L1_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFT4077874.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACH3L1_003237"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACH3L1_003237"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFT4077875.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACH3L1_003238"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACH3L1_003238"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFT4077876.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACH3L1_003239"
     CDS             complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACH3L1_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFT4077877.1"
                     /translation="MSIIILATISTTQENK"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="ACH3L1_003240"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="ACH3L1_003240"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFT4077878.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="ACH3L1_003241"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="ACH3L1_003241"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFT4077879.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="ACH3L1_003242"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="ACH3L1_003242"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFT4077880.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..13067)
                     /locus_tag="ACH3L1_003243"
     CDS             complement(11871..13067)
                     /locus_tag="ACH3L1_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFT4077881.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
BASE COUNT         3421 a         3410 c         3428 g         2978 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12901 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12961 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    13021 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13081 attaaaaaat ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13141 aatactctta tcagtaatag gctggcaaaa actttattgc tttataagac gcttgcgcac
    13201 gtaagaagag agtggtatta aacaaatgta taattac
//
LOCUS       DAZXUL010000109        13181 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZXUL010000109 DAZXUL010000000
VERSION     DAZXUL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13181
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.109"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            137..1636
                     /gene="pitB"
                     /locus_tag="ACH3L1_003244"
     CDS             137..1636
                     /gene="pitB"
                     /locus_tag="ACH3L1_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFT4077882.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1685..2377)
                     /locus_tag="ACH3L1_003245"
     CDS             complement(1685..2377)
                     /locus_tag="ACH3L1_003245"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFT4077883.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2551..3264
                     /locus_tag="ACH3L1_003246"
     CDS             2551..3264
                     /locus_tag="ACH3L1_003246"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFT4077884.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3296..4054
                     /locus_tag="ACH3L1_003247"
     CDS             3296..4054
                     /locus_tag="ACH3L1_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077885.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            4100..5449
                     /locus_tag="ACH3L1_003248"
     CDS             4100..5449
                     /locus_tag="ACH3L1_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFT4077886.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5449..6273
                     /locus_tag="ACH3L1_003249"
     CDS             5449..6273
                     /locus_tag="ACH3L1_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077887.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            6285..6845
                     /locus_tag="ACH3L1_003250"
     CDS             6285..6845
                     /locus_tag="ACH3L1_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFT4077888.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6876..7946
                     /gene="lptF"
                     /locus_tag="ACH3L1_003251"
     CDS             6876..7946
                     /gene="lptF"
                     /locus_tag="ACH3L1_003251"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFT4077889.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            7943..9022
                     /gene="lptG"
                     /locus_tag="ACH3L1_003252"
     CDS             7943..9022
                     /gene="lptG"
                     /locus_tag="ACH3L1_003252"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFT4077890.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9058..10229)
                     /locus_tag="ACH3L1_003253"
                     /pseudo
     CDS             complement(9058..10229)
                     /locus_tag="ACH3L1_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(10229..10477)
                     /locus_tag="ACH3L1_003254"
     CDS             complement(10229..10477)
                     /locus_tag="ACH3L1_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFT4077891.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10510..11424)
                     /locus_tag="ACH3L1_003255"
     CDS             complement(10510..11424)
                     /locus_tag="ACH3L1_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFT4077892.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11421..13109)
                     /locus_tag="ACH3L1_003256"
     CDS             complement(11421..13109)
                     /locus_tag="ACH3L1_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFT4077893.1"
                     /translation="MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         3164 a         3237 c         3425 g         3355 t
ORIGIN      
        1 gaatgaatgc ttattgtctg atacacaaga aataacactc tttttatcgt taaaaaatga
       61 tatttcactt tgcccatgcc gttaaagtca ctgataatgc gtccgttcgt aaattcaaaa
      121 tggcgtaatc taatatatgc taaatttatt tgttggcctt gatatatata cggggctttt
      181 gttattactt gctctggcat ttgtgttgtt ctacgaagca atcaatggtt ttcatgacac
      241 ggcgaatgcg gtggcaaccg ttatttatac tcgtgccatg cagccacaac ttgctgtggt
      301 gatggcggca ttttttaact tttttggcgt gttattgggc ggacttagcg ttgcctatgc
      361 cattgtccat atgttaccaa tcgatttgtt gctgaatatg gggtcaactc acggtttggc
      421 gatggtcttt tccatgctgc tggcggcgat tatctggaac ctgggaacgt ggttctttgg
      481 tttaccggcc tccagttcgc acaccttgat tggtgcgatt atcggcatcg gtttaaccaa
      541 cgcgctgtta accggctcat cggtgatgga tgcgttaaac ctgcgtgaag taaccaaaat
      601 tttctcctcg ctgattgttt cccctatcgt cggcctggtc attgcgggtg gtctgatttt
      661 cctgctgcgc cgtttctgga gtgggacgaa aaagcgtgac cgtattcacc gcattccgga
      721 agatcgcaaa aagaaaaagg gcaaacgtaa accgccattc tggacgcgta ttgcgctgat
      781 tgtttccgct gcgggcgtgg cgttttcgca cggcgcgaac gacgggcaga aagggatcgg
      841 cctggtaatg ctggtactgg tggggattgc ccctgctggc ttcgtcgtca atatgaatgc
      901 gtccggctat gaaattaccc gtacccgcga tgccgttacc aacttcgaac actacctgca
      961 acagcatcct gaactgccgc agaagttgat tgcgatggaa cctccattgc ctgcagcatc
     1021 gactgatggc acgcaagtaa cagagtttca ctgtcatccg gcaaatacct ttgatgcgat
     1081 tgcgcgcgtt aaaacgatgc tgccaggcaa tatggaaagt tacgagccgt taagcgtgag
     1141 tcagcgcagc cagctgcgcc gcattatgct gtgcatctcc gatacctccg cgaagctggc
     1201 gaaactgcca ggcgtcagta aagaagacca gaacctgctg aaaaaactgc gcagcgatat
     1261 gttaagcacc attgagtacg ctccggtgtg gatcatcatg gcggtagcac tggcgctcgg
     1321 cattggcacc atgattggct ggcgtcgtgt agcgatgacc atcggtgaga agattggtaa
     1381 gcgcggcatg acgtatgcgc aaggcatggc ggcacaaatg acggcggcag tgtctatcgg
     1441 ccttgccagt tatattggga tgcctgtctc cacaacacac gtcctctcgt ctgcggtagc
     1501 agggacgatg gtggtggacg gcggtgggtt acagcgtaaa acggtaacca gcatcctgat
     1561 ggcgtgggtg tttactttac cggcggcaat ttttctttct ggtgggctgt actggatagc
     1621 attgcagttg atttaacctt cctgaaaatg cccggtcctg gcgatcgggc atttccattt
     1681 ttgactagtg ataaccacgc gcggtcataa aatccgtaat cgctttttct gcatcaacca
     1741 acacctgttc cagtggctga ttggcatcga tatcaaccag ttgtgcacca ccaaaggtta
     1801 actgtggcgt tatggcaatc ttcctcgcca gcgattcccg tttatggtcg ggtttacgtg
     1861 cacaggcaac ttcaaggtca acattgagtt tgatgaccag atcaggctta tggctcgcca
     1921 tccagtgaaa cgctttacgt tcctggcttg ccagccatga gacaaaacga ccaccttcaa
     1981 cgttaggtgg gaacaccgta ccatcgtaag cgccaggaat ttggtcctga ggataacggt
     2041 cggttagaac aattaacccg cgacgacgac aggcaagcat atgacgaaag cgcagtaagc
     2101 gacgggcgac aaacgctgta attaccagcg ccggaactgg tccaggcaat ttttttgctg
     2161 ttttcacctg atttcgttca attgttttat gtaaggattt tcccatcaaa ggtaattttg
     2221 tcactgcacg accgacattt ccggcctgtt ttcctaaatg aactctttcg gcagcaccat
     2281 atttttcgac aacggtaata agatgttcac acaccgttga cttgcctgaa gcatcgctac
     2341 caataacggc aattaatgga ggtgttattg actgcatgaa tatatccttt aatttaaata
     2401 tccattaaaa atatttattt ggttaatatg tttttatgaa agcgtaattc aggtcaatgt
     2461 cacaattagt cattgtcaca ataaggttga acggatattc tgagcacgga ccacgtaata
     2521 ttcaatacat tatttactgc cgggtttttc gtgaatcaac gtaatatgtc aataattaat
     2581 tccacgccag tgcgtgttat tgccattgta ggatgtgatg gttcaggtaa atcgaccctc
     2641 acggcaagcc tggtaaatga actggcagca agaatgccaa cagaacacat ttatctcggg
     2701 caatcgtccg ggcgaattgg cgaatggatt tcacagctcc ctgttattgg cgcaccttgt
     2761 gggtgttatc tgcgaagtaa agcggcacat gtacacgaaa agcccttaac tccgcctggc
     2821 aatattactg cgctggttat ctatctgctt tcctgctggc gggcgtacaa gtttcgcaaa
     2881 atgttgtgta aaagccagca aggctatctg ctcatcaccg accgctaccc gcaggttgaa
     2941 gtgccgggtt ttcgctttga tggcccgcaa ttggcaaaaa ccacgggcgg taacggttgg
     3001 ataaaaatgt taaggcagcg cgagttgaag ctgtaccaat ggatggcgtc ttatttaccc
     3061 gtattgttga ttcgtcttgg cattgatgaa caaaccgcgt ttgcgcgtaa acctgaccac
     3121 caactggcag cgttacagga aaaaatcgcc gttacgccgc aactgacatt caatggcgcc
     3181 aagatccttg aactggatgg tcggcaacca gccgatgaaa ttatgcaagc gtcactacgc
     3241 gcaattcacg ccgcgctttc ctgatcacca aaatggatag gggatgattt aacgaatgga
     3301 tgcactacaa acacaaactg ttaatagtac aaccgcgccg cagcccaact acattccggg
     3361 gctgattgcg gtggtcgggt gtgatggcac cggtaaatcc acgctgacca ccgacctggt
     3421 gaaatcgctg caacaacact ggcaaaccga gcgccgctat ctggggctgc tctccggcga
     3481 agacggcgac aaaatcaaac gattgccgtt ggttggcgtc tggctggaac ggcgattggc
     3541 ggccaaatcc tcgaaaaccc aaagcatgaa aaccaaatct ccggcgctat gggcggcggt
     3601 gattatgtac tgcttctcgc tgcgaagaat ggcgaatcta cgcaaggttc agcgactggc
     3661 gcaaagtggc gttctggtgg tcagcgatcg cttcccgcag gctgaaattt cgggctttta
     3721 ttatgacgga ccggggatcg gcgtcgaacg tgcgaccggg aaaatcagca tgtttctggc
     3781 gcagcgcgaa cggagtttat accaaaaaat ggcgcaatac cgtcctgaat taatcattcg
     3841 tttggggatt gatattgata ctgccatctc ccgcaagcct gaccatgagt atgccgagct
     3901 gcaggacaaa atcggcgtca tgtcgacgat tggctataac ggcacaaaaa ttcttgagat
     3961 tgattccaga gcgccctaca gcgaagtgct ggaacaggta cagaaggcgg tttctctggt
     4021 tgccatcgtt tctgaccgcc gaagtttaac ttaaggctgg cgggatattt tttactatca
     4081 acgataaggt caggctgaat tggcgggttt taacatcaaa cattggtttg cagatggcgc
     4141 gtttcgcacc attattcgca atagcgcctg gttaggctcc agtaatgtcg agagcgcctt
     4201 gctggggctg ttggcgctct cgtgtgccgg taaagggatg acgcccgcca tgtttggtgt
     4261 actggtgatt gtgcaatcgt acgccaagtc gatcagcgat tttattaagt ttcagacatg
     4321 gcagctggtg gttcagtacg gaacgccagc cttgaccaac aataatccgc agcaatttcg
     4381 caatgtcgtc tcattttcct tctcgctgga tatcgtcagc ggcgcggtgg cgattgtcgg
     4441 cggcattgcc ttactgccat tcctttccca ttcattaggt ctggatgacc aaagcttttg
     4501 gctggcggcg ctgtattgca cgctcattcc ttcaatggct tcctccacgc cgaccggcat
     4561 tctgcgtgcg gtagatcgct ttgatttgat tgctgtacag caggcgacga aaccttttct
     4621 gcgcgcagcg gggagcgtcg cagcctggta ttttgacttt ggttttgtgg gttttgttat
     4681 tgcctggtac gtgtcgaatc tggttggcgg caccatgtac tggtggtttg ccgcgcgcga
     4741 attacgccgt agaaatatcc ataacgcctt caaattgaat ctgtttgagt ctgcccgaca
     4801 cattaaaggc gcgtggagtt ttgtctggtc aaccaacatt gcccactcca tctggtcggc
     4861 gcgtaactcg tgcagcacag tgttggtggg gatcgtgtta ggacccgctg ccgccgggtt
     4921 atttaaaatc gccatgacat tcttcgacgc cgccggaacg ccagcgggtt tgctgggtaa
     4981 aagtttttac ccagaggtga tgcgtttaga tccgcgcacc accagaccgt ggttgttagg
     5041 tgtgaagtcc ggtttactgg cgggcggaat cggtattctg gtggcgctcg cggtgttgat
     5101 cgtcggcaag ccgctcattt cgctggtgtt tggcgttaag tatctcgaag cgtatgacct
     5161 gattcaggtg atgctgggcg caattgtgat ttcgatgctg ggcttcccgc aggagtcatt
     5221 gctattgatg gcgggaaaac agcgcgcttt tctcgtggcg caaaccatcg cctcaatcgg
     5281 ctacatcgta ttgctgttca tgttctgtca tctgtttggt gtgctgggag cagcgtttgc
     5341 ttacttcggt gggcaatgtc tggacgtggc gctctcgtta attccaacct taaaggcatt
     5401 ttttcagcgc cattccttgc tttataacgc agccggagag aaatcctgat gatgagaaaa
     5461 tatttcccgc ttgaggcgag cgagcgtcta tttgttgcga ttgaagaaga tgatgtcgtt
     5521 gatgcacagg ttagtttgcc gccgactatt gcactaagtt gtaccaccga aatcattcat
     5581 gataattacg cgttatgcct gcaattctgg ctcaatggcg ttgatcgtca ggaattactg
     5641 cgtttagttc gcaaacaagc caaaggcgat gagctgactg ctgatgagcg caaacaattt
     5701 aaatacatgc gagcgcgcta caagcacctg cgttttgcgc aacggctgta tttaaagaag
     5761 catcaggccg gatttttatt cggcaaaacg accgtttttc tggggcgttt tcaggacggc
     5821 tttcgtaacg gtaagaaaaa tattgtgtcg tattacggca acctgttacg gatttattta
     5881 agctctccgg tctggtcgtt ggtgaactat tcattacgcc atagccagtt agaaagcgta
     5941 agcagtttta ttgcctatcg gcaaaagcaa atgcataccc tgaaagagat tattgccaaa
     6001 ccgcgactaa ccggcaggga atttcatgat gtgcgaaaaa tcatcagcca gcaggtttct
     6061 tattacgaca ccttaagatc gcttgatcct gaaaataaag aggcgttaca gatttcccgt
     6121 ttcctcgccg ccattaatgg cctgatggga gataaacacg acgacatggt ggcggatgat
     6181 atggaaaacc gccagtcgta ttatgcaccg ttggcgctgg atagcgatat ttgtcagcgc
     6241 ttagagctgc tgatttcgcg ttttccgcta tagccggagc aatgatgtca tctcgtttga
     6301 ttattgcgtt gattatcatg ctcttagccc cgggtgtgca ggcacacaat ttcgtcactg
     6361 gcaaaacggt tacccctgtt tatatccagg aaggcggcga gctgcttctg aatagcgacg
     6421 acgaaattca ctaccaaaag tggaacagca cacaattagc gggaaaagtc cgcattattc
     6481 agtatatcgc cgggcgtaag tcggcgaaga aaaagaactc actgttaatt aaagcggttg
     6541 aagcggcaaa ctttccgcag gatcgttttc agcccaccac cattgtgaac accgatgatg
     6601 ccatctttgg tactggctat tttgttgttg gcaaaatcga aaaaaataaa cgccgctatc
     6661 cgtgggcgca attcgttatt gacggcaatg gacagggacg cgttgcctgg cgcttgccgg
     6721 agcagagttc aaccattctg gtgctgaata aagccgggca aattcagtgg gcaaaggacg
     6781 gcagcctgac gccggaagag gtagatcatg tcattgcgtt ggcgcagaaa ctgatcaatg
     6841 aataaaaaac tcaaaggatt gagggcgtta gtgagatgaa gctggttgaa cactacatca
     6901 tgcgtggtac gcgccgtctg gtgctgatta tcgtcggttt tctgatcttt attttcgcca
     6961 gctactccgc acagcgttat ctgaccgaag cggcaaatgg gacattagct cttgatgttg
     7021 tgctggatat cgttttctac aaagtactga ttgcactgga gatgttgtta cctgttggtc
     7081 tgtatgtgtc agttggcgtg acgctagggc agatgtatac cgactcggaa attaccgcta
     7141 tctctgcggc ggggggcagt ccgggacgct tgtacaaagc cgttctttat ctggcgatac
     7201 cgctaagtat ttttgtcacc cttctgtcga tgtatggtcg tccgtgggct tatgcgcaga
     7261 tttatcaact ggagcagcag tcgcagtcgg agctggatgt tcgccagttg cgggcaaaga
     7321 aatttaacac taacgataac ggacgaatga tcctttcgca gacggttgat caggataata
     7381 atcgcctgac tgacgcgctg atttatactt ctactgccaa tcgaacccgt attttccgcg
     7441 ccggttcggt tgatgtggtt gacccatcac ccgagaaacc gaccgttatg ttgcacaacg
     7501 gcactgccta tcttctcgat catcaggggc gtgacgacaa cgaacagatc taccgtaatc
     7561 tgcaattaca tctgaatccg ctggatcaaa gcccgaacgt caaacgcaaa gcgaaatcgg
     7621 tcacggagct ggcgcgctcc gcctttcctg ccgatcatgc cgaactgcaa tggcgacaaa
     7681 gccgtggcct gaccgcattg ttgatggcgc tgctggccat ttcattaagt cgggtaaaac
     7741 cgcggcaagg gcgattttca acgttattgc cactgacgtt gctgtttatt gccatttttt
     7801 atggcggcga cgtctgccgt acactggtgg ctaacggcgc gattcccctc attcctggtt
     7861 tgtggttagt gcccggactc atgctaatgg gcctgctgat actggtcgca cgtgacttct
     7921 ctttgctgca gaaattttcc cgatgaatgt tttcagtcgc tatttaatcc gtcatctctt
     7981 tctcggtttt gccgccgctg cagggctatt gctgccgctt tttaccacct tcaacctgat
     8041 taacgaactg gatggtgtca gcccgggcgg ttatcgctgg actcaggcgg tgctggtggt
     8101 gctaatgacc ttaccgcgca cactggtcga actttcgccg tttatcgcgt tattgggtgg
     8161 tattgttgga ctggggcagt tatcgaaaaa cagtgagctt accgccattc gcagcacggg
     8221 gttttctatc ttccgtattg cactggtggc gctggttgcc gggatattgt ggactgtttc
     8281 gttaggcgcg atagatgagt gggtggcgtc gccattgcag caacaggcgt tgcaaatcaa
     8341 atcgaccgcc accgcgttgg gggaggacga tgacattacc ggcaatatgc tgtgggccag
     8401 gcgtggtaat gaatttgtga cggtgaaatc gctgaacgag cagggccagc cggtgggcgt
     8461 ggagatattt cattatcgcg acgatctctc gcttgaatcc tacatttatg cacgcagtgc
     8521 caccattgaa gacgacaaaa cgtgggtcct gcatggtgtg aatcataaaa aatggctgaa
     8581 tggcaaagaa acgctggaaa cattagataa tcttgcctgg caatcggcct tcaccagtat
     8641 gaatcttgaa gagttatcaa tgccggggaa tactttttct gtccgtcagc ttaatcatta
     8701 catccattat ttgcaggaaa ccgggcaacc cagcagcgaa taccaccttg cactgtggga
     8761 aaaactgggg caacccatcc tgaccctggc gatgattttg ctggctgtgc cgtttacctt
     8821 tagtgccccg cgttcgcctg ggatgggtag ccgtctcgct gtaggcgtca tcgtgggctt
     8881 actcacctgg atcagctatc aaatcatggt taatctggga ttgctatttg cgttaagcgc
     8941 acctgttacc gcgctcggtt taccggtagc gtttgtgttg gtggcgttga gcctggtgta
     9001 ttggtatgac agacaacatt aaacccgccg tggcgcaaac accacggcgg gctcacatta
     9061 ctgcttcaga gtagcaaatg cctgaatgat ggcatcgatt tgctcacgac tgtgggcagc
     9121 atttacgcta cagcgcagca gcgtgatgcc tgcaggcgcg gcaggcggca ggataagatt
     9181 cacatacacg cccggcgaga taagctcgcg ccagatgcgt aaaccttcct ctttcgaacc
     9241 gattattact ggcactaccg ggctaatatg tgaacttaac tcataaccca gtttttgcag
     9301 accgtcatac agatggttgg cgttatccat cagcttttgc cgcagctctg gttgtgtggc
     9361 aatagttctt aacgacgagc gcacggtcgc aatgcaggac ggagaaggag aggcggtaaa
     9421 aatatacggg cggctgccgt agcgcagcac ttccatcact tccgaaacga cagcaaaacc
     9481 gccaatcgag gccagacttt tactgaaagt accaacgata atatccacat catcttcaac
     9541 gccgacagct tcagccagtc cgcgccccgt tgcgcctaac acgccaaacg aatgcgcttc
     9601 atcgacaatc aaataaccac cgagacgacg tttgatatca acaatttccg ccagcggcgc
     9661 aacgtcaccg agcatgctgt agatgccctc aacgatgata atcgcctctt tcgcccgttc
     9721 accgaggcga accatgcgcc gttccagatc tttagcatcg ttatggcgga aacgaataat
     9781 ttccgcgcca ccgagagaac agacgtcata aatactggcg tggctgtcgg catccagtaa
     9841 caccacggcg ttatggtcgg caagtgcgct gatgacgcct aaattagcgg tataaccggt
     9901 tgaaaaacga tagcggtagg gcgattgaaa aactcggcaa tctctttctc taacgccagg
     9961 tgcggcgcat aactgccgtt tgccatacga gaaccggtgg tgccggtgcc ttgggccgct
    10021 aacgcggcct gaccttcgct gatggcatca tgattaaagg ttaaaccgag ataattattg
    10081 gtcccggcca gaatcacttt tttattgccg atccggcctt cggtggcgga atacacttca
    10141 tcaatacagg tgccaaacgg tgttaacccg ttgtcactaa attgcagacg ctcgccagca
    10201 aggcgcgcat atttatcgta tagccccatt atttattctc cagccatgga agtagagttt
    10261 ccattaattg cgcaggggtt ttgacatcca gcagaatatt aataggaatg gaaatatcga
    10321 atctgtcttc cagcatcatt aagagatcca tcactttgat cgactccagg ccaagatcgt
    10381 tgacgagatc gctgtcgggc ttaatttcca caccgttttc gaccaaatcc tgtaaacagg
    10441 aaaggatgta atccattact atttcacgat ttaccataga aacgttaaca cacctttaaa
    10501 acaagacaat caaaatagcc cgttgcgtaa tgcgtgttcc aggctaatcc cgggaaacca
    10561 attgatatct tcagaaatac ggttattact tgccgaccag tcggcgtggg ttaattcacg
    10621 aattttcgag cgggtcagca taggttcttt acctgccagg cgactcaacg cggtactgat
    10681 atccgcaagg cctgtgagta ccggcagagg aatgtcaacc attcgcacgg aaccacaacg
    10741 ggctttggcg gcaagctgtc gtacgcgttg ccagtcatag ccaccggcga cgccatcgca
    10801 taattcatag gtttgcgtct gtacagtttc ggcgcttaac cactgaccca ctgcttgcgc
    10861 aaaatcggtg acgtgaagaa aagagagttg tgtatctggc gcaccaagtc gtggcagcag
    10921 gccgcgtagc atccagtcaa acagcggttt taactcttta tcgccgggac catacacggc
    10981 tgtcgggcga aaaacgccca gcgtaatttc gtccgccatt gctgccagtc gttgctcggc
    11041 gacgtatttg gattttgcat accaggagag ctcgggatgg cgcgccgcca gcgaagagat
    11101 aaacagaaaa cgttggcaaa agccgctctc ttttgctgct tgcatcaggc gcaggctgcc
    11161 gtcaacgtta cagcgggtga aaatctcttc tttgtgcccg cgcacttgcc cggcgcaatg
    11221 gaccactacg ctggctccgg caaccagctc gctaagtgaa tgagtatctt ccagcgaacc
    11281 gcgcacccag gtgagattat cattgacgtg agcgcgggca gtacgcgtca atgcgcgaac
    11341 gtgaaagccg cgggcgagca ggttatcgat aatatattta ccgataaacc cggtagcgcc
    11401 cgtcaccgcg acagtttgat ttatgccagg gattcctgca cattaagact ggcagcataa
    11461 gccttctgat aacgtttttt cgcttccgca cgggcaggct tgccggagga cgttcgggga
    11521 atactgtggg gcggcaacag atcgatagcc gcggtcacgc caaattcgct ttggatccgt
    11581 gccgccagcg cgtggataag ctgcccgcga cgttcttcgt cgctgatccg acactggatc
    11641 tgcaaaatga ttttttcctg ggcggtaaca aaagcaatcg catcgccaga atgaatttcc
    11701 ggttcctgtt ccgctatata ttcaatatcc tgcggccaga tattacggcc acgaataata
    11761 atcagatctt taatgcgtcc ggtgacatac agataaccgt ccagcagata accgaggtcg
    11821 ccggtgtcta accagcccgt cgcggcaatc tcgtcttgcg aaacctggtc gccaaagtaa
    11881 ccgctcatca gactgggacc ggagatgcaa atatggccta ccacacgttc cgcgaccggc
    11941 atacctgctt cattgcggat ttcaatacca tgttccggca acgctttgcc gcagttgacg
    12001 aaagtcgata cggcgcgtgt ctctgcaccc ggcgcgacgg ctttaccctg atattcgagg
    12061 atgtcgcgat ccacttcgtt aaccacaacc ccggaggctt catcagagaa gctgacagcc
    12121 agcgcatttt ctgccagtcc gtagcacggc atgaaagttt tattgtcaaa gttaacctga
    12181 cggaaacatt cagcgaattg atggagttgt tctgcggaga tcggctcagc accaatacca
    12241 gcgacgcgcc agcaggaaag atccagttca gcgagatctt tttcattcac gcggcgttgg
    12301 cacaattcat agccaaacgg cggcgcaacg gaaacggtgc cgcgattttt actgatcaat
    12361 ttaagccatt gcaaaggacg catggcaaaa tcctgagtgc gcaaataatc tactgaaagt
    12421 tgcgtggcga cggggggcag gagaaagccg accagtccca tatcatggta gaaaggctgc
    12481 caggagacgc agcggtcgct agggcgtaat ttaatgccat cgtggcttat agcacgtaga
    12541 ttagccatca cttcgcgatg ggtgataatg acgccacggg gaaaacgggt gctgccggag
    12601 gtgtactgga ggtaggcgat atcgttcgga actggacgct ggagcgcaac atcggcttcc
    12661 ggtaatgcct taaaccaagc gtggcttaaa acatgtaatt cggggttgtt atgcgtcgcg
    12721 gcattgacca gtggcaacca ctcatcacca gtgataatgg ctgcgggctg gcaacttgcc
    12781 agtaaaccct ggagtttggc gctccaggaa tcccgctgac cgacgcccat tggaatcgcc
    12841 aacgggacgg cgactaagcc agcatactga caggcaaaaa aagcctctac aaacccgcta
    12901 ctcgtttcgg caatcagcgc cacgcgttcg ccttttttca ggttcagcga taacaaccgc
    12961 ttcgcaccag cttcggcacg cgtttttaat gtctgatatt ccagttgatc ttcaagttgg
    13021 caacgtctgt cataaaaatt cattccggct ctactcagag cggcgtagtc caaagcatca
    13081 accagcgttg gaaaatcggc atagcgcata ggtagggaat gcgtaaagat tttattagac
    13141 atatacacca cgaaaaaatt ggatatcaaa aataaaatga c
//
LOCUS       DAZXUL010000110        13125 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZXUL010000110 DAZXUL010000000
VERSION     DAZXUL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13125
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.110"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(109..429)
                     /locus_tag="ACH3L1_003257"
                     /pseudo
     CDS             complement(109..429)
                     /locus_tag="ACH3L1_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(635..1354)
                     /locus_tag="ACH3L1_003258"
                     /pseudo
     CDS             complement(635..1354)
                     /locus_tag="ACH3L1_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1370..1702)
                     /gene="cusF"
                     /locus_tag="ACH3L1_003259"
     CDS             complement(1370..1702)
                     /gene="cusF"
                     /locus_tag="ACH3L1_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFT4077894.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1726..3108)
                     /gene="cusC"
                     /locus_tag="ACH3L1_003260"
     CDS             complement(1726..3108)
                     /gene="cusC"
                     /locus_tag="ACH3L1_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFT4077895.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3265..3948
                     /gene="cusR"
                     /locus_tag="ACH3L1_003261"
     CDS             3265..3948
                     /gene="cusR"
                     /locus_tag="ACH3L1_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFT4077896.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3938..5380
                     /gene="cusS"
                     /locus_tag="ACH3L1_003262"
     CDS             3938..5380
                     /gene="cusS"
                     /locus_tag="ACH3L1_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFT4077897.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5530..7767
                     /gene="nrfB"
                     /locus_tag="ACH3L1_003263"
     CDS             5530..7767
                     /gene="nrfB"
                     /locus_tag="ACH3L1_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFT4077898.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7754..10722
                     /gene="nfrA"
                     /locus_tag="ACH3L1_003264"
                     /pseudo
     CDS             7754..10722
                     /gene="nfrA"
                     /locus_tag="ACH3L1_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10723..11613
                     /locus_tag="ACH3L1_003265"
     CDS             10723..11613
                     /locus_tag="ACH3L1_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFT4077899.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11796..12557
                     /gene="envY"
                     /locus_tag="ACH3L1_003266"
     CDS             11796..12557
                     /gene="envY"
                     /locus_tag="ACH3L1_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFT4077900.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12603..12679)
                     /locus_tag="ACH3L1_003267"
     tRNA            complement(12603..12679)
                     /locus_tag="ACH3L1_003267"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12643..12645),aa:Arg,seq:tct)
     gene            12922..>13125
                     /locus_tag="ACH3L1_003268"
     CDS             12922..>13125
                     /locus_tag="ACH3L1_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFT4077901.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3218 a         3405 c         3331 g         3171 t
ORIGIN      
        1 atccctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
       61 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcagt tgtcgaacca
      121 gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa aataagtgcg
      181 tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc tggcatcctt
      241 caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat gccagcgata
      301 gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc catcagcatc
      361 agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt ttgtgtcgct
      421 tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac atctggtaca
      481 caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg tagacatact
      541 caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact acagtggaca
      601 atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg accacgttat
      661 ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg
      721 ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga atatccgcct
      781 ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc gccgtaccgc
      841 cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga atggtcaggt
      901 cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac accttgtcga
      961 taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa ctgacattcg
     1021 tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt ttcaccccca
     1081 gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct tcatcggcat
     1141 atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg aaccgcgtat
     1201 tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc attttttctg
     1261 cgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata ccgcccgcga
     1321 tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt tactggctga
     1381 ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac gccactttgt
     1441 cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc atggtcatct
     1501 ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg ctttccagat
     1561 caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg ctcatggttt
     1621 catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga ctgaacattg
     1681 cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt gctgccaacc
     1741 gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat tcaggtcaag
     1801 taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca gataacttac
     1861 cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg acgccagata
     1921 acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg catctgccac
     1981 ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct gctggcgaat
     2041 ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga tctcaatttt
     2101 aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat cgctgctggc
     2161 ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg cagcaccaat
     2221 attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc gctgcaataa
     2281 gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc tgtttactgt
     2341 ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat tattcgcctg
     2401 cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca ccccgcgagc
     2461 ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga caaacgcata
     2521 tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat acgccagctg
     2581 ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg cgcgctgggc
     2641 ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta agcgaccgaa
     2701 aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg tggctgaatc
     2761 gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg ggtagcggtc
     2821 ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg ccatgcgcaa
     2881 atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat caacaaaaaa
     2941 ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt tctggctgag
     3001 tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca gtgaacaacc
     3061 agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag gctcataatt
     3121 tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg tgacaggaaa
     3181 atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta ctctagaatt
     3241 gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc
     3301 ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt ggccgacaac
     3361 gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat cctcgatatt
     3421 atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc caataaaggg
     3481 atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa ggggctggag
     3541 ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct ggcgcgggtg
     3601 cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca ggttgccgat
     3661 ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat cactctgacc
     3721 agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt gctgccccgc
     3781 tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa cgccattgat
     3841 gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa gctgattcaa
     3901 accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc catttcagcg
     3961 cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca tcgcggcgtt
     4021 tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg agcaggacat
     4081 taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc ctgacgaaac
     4141 gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca acgtgttgat
     4201 ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc ctgatatccg
     4261 cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg tgtatctcct
     4321 ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac acagcaactg
     4381 gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt atacgctcta
     4441 catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga ataaacttat
     4501 tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg cggtacataa
     4561 aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct cgaaagatct
     4621 cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg tactgtcgtt
     4681 caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt tctcagcgga
     4741 tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg aaatcgccct
     4801 cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc tcgaagagct
     4861 gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg ataacaacca
     4921 gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt
     4981 tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg acaaacgtca
     5041 ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt ctaatgccct
     5101 tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg atcaccaggt
     5161 gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac cgcgattgtt
     5221 tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta gcggtattgg
     5281 gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg taacgtcaga
     5341 tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca aacggcaact
     5401 tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg gtaaaaagct
     5461 gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc gttaatgtaa
     5521 ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg cttaaaagta
     5581 atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt ttttattgat
     5641 gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg ctacccgcga
     5701 atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat ggttccggcg
     5761 tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac gctcgactac
     5821 gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca acgtgatgtt
     5881 gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg tcctggtccc
     5941 accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt tgaacgtagc
     6001 gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat ttcaccgatg
     6061 gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat cccagtgtat
     6121 ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga gttttcagaa
     6181 ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc cagcgcaggc
     6241 gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg taacggtatt
     6301 gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct gaaagaaaaa
     6361 ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga acgcgagcag
     6421 cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga atatttcccc
     6481 gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat tgttttccag
     6541 ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct ctggcgcgac
     6601 cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat gatccagctt
     6661 ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct ttctattttt
     6721 agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat ggttaaccgc
     6781 atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg cctgctttcc
     6841 gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg cgtgctgaaa
     6901 caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac gcatgacttc
     6961 cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct gctggaaaat
     7021 caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga aggtctacgc
     7081 ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc acaggcgctg
     7141 gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc gtcctcgctg
     7201 attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct gcgtctggaa
     7261 aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct ggcggccctg
     7321 acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca aattgtcaca
     7381 gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat gttgtacaat
     7441 gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg gcaatatgtg
     7501 ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa tcgttcagca
     7561 attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa gtttttggtc
     7621 accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca acgcgaatta
     7681 caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca ggttgcgcaa
     7741 ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt ctggtttact
     7801 gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca gcgcagaaga
     7861 gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata aggcgctgaa
     7921 ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata tacaccaaca
     7981 ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc attttggtca
     8041 tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag gagatgcccg
     8101 acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga ctgttgaaga
     8161 actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc gcagtgaagt
     8221 cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac tgaacgatgc
     8281 gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc aacgggcaat
     8341 ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc agcagaacac
     8401 attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc agctggacga
     8461 tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat atattactta
     8521 cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc tcattgaaaa
     8581 taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt tatctaaata
     8641 cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg ataaccgcca
     8701 gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg acgcagcgca
     8761 aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg ctgtcagcgt
     8821 ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc agcaagaacc
     8881 gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg agcagtcacg
     8941 cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc gtgtcagcca
     9001 gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag caacgccggc
     9061 gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt ggcaaagtca
     9121 gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg atatgtcgcc
     9181 ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca cgctacccgg
     9241 tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct ggcaacatcg
     9301 tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg cggcctggca
     9361 gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca acaccgctca
     9421 ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac aacgtgagct
     9481 gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag ctcagccaga
     9541 actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca actcttacgt
     9601 tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga gtgatttgcg
     9661 cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg gttacgcctt
     9721 gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc ataaagggct
     9781 tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc tggatgacat
     9841 gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc aggcataacc
     9901 ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca tgaggaggtc
     9961 ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg cgcaatgagt
    10021 accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag ctacggacaa
    10081 ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct gctctcagtt
    10141 tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt gaaaaatccg
    10201 atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt cctcgccgtc
    10261 gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct gcgcgccagc
    10321 gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg ttcaggctgg
    10381 tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat tcaggcgtgg
    10441 acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac tattgagcct
    10501 tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc gcagcttggc
    10561 ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc ctggccgcac
    10621 aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca acgtaacgga
    10681 gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa gttcattttc
    10741 gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg tattatctgg
    10801 caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct gatgagtcag
    10861 ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg cgatgcattt
    10921 acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca acagactggc
    10981 ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca gaaacagtcg
    11041 tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca gcaagccaga
    11101 ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc ggaaattgac
    11161 gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt aaacaacgcg
    11221 cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt tttcgccgga
    11281 aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac cggagttaat
    11341 gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg tgaacgttat
    11401 ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt ttatgaactg
    11461 tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc agaaattgcc
    11521 tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc tctgcgctat
    11581 ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt cgggtacgac
    11641 ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta aaaagcatgc
    11701 taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa gcatagatcc
    11761 tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc agtgaacctt
    11821 gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat gctacgttta
    11881 cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt gaactctcaa
    11941 cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat tatctgttgt
    12001 ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc ccggttatcg
    12061 cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc aagcagcaat
    12121 ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta ttgtctaact
    12181 ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc agcgtccgcg
    12241 acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg cgaattgtcg
    12301 ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac gaaaatacca
    12361 gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg ttattgatgg
    12421 ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg tcgtacttta
    12481 tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc aaacagcgac
    12541 aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa atgaagaaga
    12601 aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc gttgctctat
    12661 ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga attatacggt
    12721 caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac gaccgcgtct
    12781 gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg cgtctgaatc
    12841 aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg aaaattgtcc
    12901 gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga tactcatcct
    12961 atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca gattgtcctg
    13021 aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt tgatttaatc
    13081 attatggata tagacttgcc cggaacagac ggttttacct tcctg
//
LOCUS       DAZXUL010000111        13098 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZXUL010000111 DAZXUL010000000
VERSION     DAZXUL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.111"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACH3L1_003269"
     CDS             complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACH3L1_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFT4077902.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="ACH3L1_003270"
     CDS             1131..1931
                     /locus_tag="ACH3L1_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFT4077903.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="ACH3L1_003271"
     CDS             2009..2779
                     /locus_tag="ACH3L1_003271"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFT4077904.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACH3L1_003272"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACH3L1_003272"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFT4077905.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACH3L1_003273"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACH3L1_003273"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFT4077906.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="ACH3L1_003274"
     CDS             5034..5756
                     /locus_tag="ACH3L1_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFT4077907.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACH3L1_003275"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACH3L1_003275"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFT4077908.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACH3L1_003276"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACH3L1_003276"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFT4077909.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="ACH3L1_003277"
     tRNA            7149..7225
                     /locus_tag="ACH3L1_003277"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="ACH3L1_003278"
     CDS             complement(7356..8402)
                     /locus_tag="ACH3L1_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFT4077910.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACH3L1_003279"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACH3L1_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACH3L1_003280"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACH3L1_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFT4077911.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACH3L1_003281"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACH3L1_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFT4077912.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="ACH3L1_003282"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="ACH3L1_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACH3L1_003283"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACH3L1_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3393 c         3484 g         3214 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtgt
//
LOCUS       DAZXUL010000112        12920 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZXUL010000112 DAZXUL010000000
VERSION     DAZXUL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12920
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.112"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..252
                     /locus_tag="ACH3L1_003284"
                     /pseudo
     CDS             1..252
                     /locus_tag="ACH3L1_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(862..1238)
                     /gene="rnpB"
                     /locus_tag="ACH3L1_003285"
     ncRNA           complement(862..1238)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACH3L1_003285"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1271..2416)
                     /gene="garK"
                     /locus_tag="ACH3L1_003286"
     CDS             complement(1271..2416)
                     /gene="garK"
                     /locus_tag="ACH3L1_003286"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFT4077913.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(2513..3403)
                     /gene="garR"
                     /locus_tag="ACH3L1_003287"
     CDS             complement(2513..3403)
                     /gene="garR"
                     /locus_tag="ACH3L1_003287"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFT4077914.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(3433..4203)
                     /gene="garL"
                     /locus_tag="ACH3L1_003288"
                     /pseudo
     CDS             complement(3433..4203)
                     /gene="garL"
                     /locus_tag="ACH3L1_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(4219..5553)
                     /gene="garP"
                     /locus_tag="ACH3L1_003289"
     CDS             complement(4219..5553)
                     /gene="garP"
                     /locus_tag="ACH3L1_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFT4077915.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5928..7499
                     /gene="garD"
                     /locus_tag="ACH3L1_003290"
     CDS             5928..7499
                     /gene="garD"
                     /locus_tag="ACH3L1_003290"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFT4077916.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(7551..8375)
                     /gene="agaR"
                     /locus_tag="ACH3L1_003291"
     CDS             complement(7551..8375)
                     /gene="agaR"
                     /locus_tag="ACH3L1_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFT4077917.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            8624..9904
                     /gene="kbaZ"
                     /locus_tag="ACH3L1_003292"
     CDS             8624..9904
                     /gene="kbaZ"
                     /locus_tag="ACH3L1_003292"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFT4077918.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9927..10400
                     /gene="agaV"
                     /locus_tag="ACH3L1_003293"
     CDS             9927..10400
                     /gene="agaV"
                     /locus_tag="ACH3L1_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFT4077919.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10411..11190
                     /gene="agaW"
                     /locus_tag="ACH3L1_003294"
     CDS             10411..11190
                     /gene="agaW"
                     /locus_tag="ACH3L1_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFT4077920.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11180..11989
                     /gene="agaE"
                     /locus_tag="ACH3L1_003295"
     CDS             11180..11989
                     /gene="agaE"
                     /locus_tag="ACH3L1_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFT4077921.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            12076..12510
                     /gene="agaF"
                     /locus_tag="ACH3L1_003296"
     CDS             12076..12510
                     /gene="agaF"
                     /locus_tag="ACH3L1_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFT4077922.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12507..>12920
                     /locus_tag="ACH3L1_003297"
     CDS             12507..>12920
                     /locus_tag="ACH3L1_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFT4077923.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         3144 a         3446 c         3246 g         3084 t
ORIGIN      
        1 tacaatcatt gaagattatg aaaatatcaa agaaaatgct tatctggtcg tcccccaaat
       61 taattatact atggatttaa acatagaaga ctctagctca gaagagttaa taccctcgag
      121 aaagacgaaa attctccgga caatggcttt gaggtcgggg aatataacac atatgaagca
      181 tataactcag agaatcaata ttttaccaga gaggactata cgtataatta cgacctttta
      241 aatgcaatat agacttaata acttcttaca aagtctatta ttaataccgt agatattcat
      301 cttaccctgt gcaccaaaag caaaaactta tcgacgcggg tttgaaactg atggcaaccg
      361 caaaaatgcc tgatgcgcta cgcttatcag gcctacgcca tctctgcaat atattgaatt
      421 tgcgtgcttt tgtaggcagg ataaggcgtt cacgcagcat ccggcatgaa taaagcgcac
      481 tcaacaatct gaaacccgcc ggaacggttt attacgtaca tcaggtgaaa ctgaccgata
      541 agccgctttc ttttgggtat agtgtcgtgg acagtcattc atctttctgc ccctccaaaa
      601 gcaaaaaccc gccgaagcgg gtttttacgt atatcaggtg aaactgaccg ataagccgct
      661 ttcttttggg tatagcgtcg tggacagtca ttcatctttc tgcccctcca aaagcaaaaa
      721 cccgccgaag cgggttttta cgtacatcag gtgaaactga ccgataagcc gctttctttt
      781 gggtatagcg tcgtggacag tcattcatct ttctgcccct ccaaaagcaa aaacccgccg
      841 aagcgggttt ttacgtaaaa caggtgaaac tgaccgataa gccgggttct gtcgtggaca
      901 gtcattcatc taggccagca atcgctcact ggctcaagca gcctacccgg gttcagtacg
      961 ggccgtacct tatgaacccc tatttggcct tgctccgggt ggagtttacc gtgccacgga
     1021 ctgttaccag ccgcgcggtg cgctcttacc gcaccctttc acccttacct gatcccgctt
     1081 gcacgggcca tcggcggttt gctctctgtt gcactggtcg tgggtttccc ccccaggcgt
     1141 tacctggcac cctgccctat ggagcccgga ctttcctccc ctccacccgt ctcccccgaa
     1201 gaggacgacg acgaagcggc gactgtctgg tcagcttcgg cgcgcagtat agagggtttg
     1261 cgcgcccttg tcaccccgtg ttgcgcattc caatcgccag tgtcgcggca atattacgtg
     1321 aagcacggca gatattgtca taagccccgc ggaatgcttc gtccaacgta cctatgctgg
     1381 tcaatacgct gaagaccgca tcaatgccat gctgatgtac aacgccaaca tcatcggtca
     1441 ggctacccgc aatgccaatc accggtttat ggtacttctt cgccacgttt gcgacgccaa
     1501 tcggtacctt gccgtgaata ctctggctgt caatacgccc ttcaccggtg atcaccagcg
     1561 tacaatcgtg aatatgttcc tccagattca gcgccgtagt gacgatttca ataccacttt
     1621 tcagttccgc accaagaaac gccatcagcg ccgcacccat accgcccgca gctcctgcgc
     1681 cggggacatc ttttacatca acatgcagcg cttttttaat gacatcggca tagtgagaga
     1741 ggttattgtc cagctcaaca atcatcgctt cactggctcc cttttgtggg ccaaagatgc
     1801 gcgatgcgcc gttatcgccc accagcggat tggtgacatc acaagcgacg cgaatgacgc
     1861 aatcttttaa gcgcggatcg aggccggaaa tatcaatatc attcagagta ttaagactac
     1921 cgccgccaaa accaatttca ttgccgttgg cgtcgcataa tttcgccccc agcgcctgta
     1981 ccatgcctgc gccgccatca tttgtagcgc taccgccaat gccgataata atgtttgtcg
     2041 cgccgctctc cagcgcctgc aggattaact cgcctgtgcc gcgtgaagtg gtcacgagtg
     2101 gatcgcgttt ttccgcaggt accagctcca gcccactggc cgccgccatt tcaataaacg
     2161 cggttttgcc atcgccggag atcccccaac tggcattcac tttctcgccc agcggccctg
     2221 taacccaggc gtgacgttca gaaccctggg tggctgcaat catcgcttcc accgttcctt
     2281 cgccaccgtc ggcaaccgga atagaaacgt actgtgcatc aggaaaaatt tcccgaaatc
     2341 ctttttctat cgcctgcgca acctcgctgg cagataaact ttctttataa gagtctgggg
     2401 cgattacgat tttcatacct atgcctgtta ccacatgacg ccggagggcg tttctcttat
     2461 tcggcctgga ttccaggccc ggattgcaat acgccatccg ggcacgacgt cattaacgag
     2521 taacttcgac tttcgccagt ttttcgtagt agcacaccag ggcgctatga tccgccgttc
     2581 ctaaaccatc tgctcgcagt gcctgcatca tctccataac cgcagctgtg agcggcagtt
     2641 gcgcgccgac gccgtgggaa gtatccagcg catttgccag atccttaata tgcagatcaa
     2701 tacggaagcc cggcttgaag ttgcggtcca tcaccatcgg cgctttggca tccagcacgg
     2761 tactgcccgc cagtccaccg cgaattgcct gatacaccag gtccgggtta acgcccgctt
     2821 tagttgccag cgttaacgct tctgacatcg cggcaatatt cagcgccaca atgacctgat
     2881 tcgccagttt ggtgacgtta cctgcaccaa ttgccccggt atgcaccacg gaacccgcca
     2941 ttgctttcat caaatcatag tatttgtcga aaatagcctt gtcgccgccc accatcactg
     3001 acagcgtacc gtcgatggct ttcggttcac cgccgctcac cggagcatcc agcatatcaa
     3061 tgcctttcgc tttcagcgct tcgctgattt cacggcttgc cagcggtgcg atagaactca
     3121 tatcgatcaa taccgtacct ggcttcgcgc cttcaataat gccattctca cccagcgcca
     3181 cctctttcac atgaggggag tttggcagca tggttatgat gacgtcgcac tgttcagcga
     3241 tcgctttagc cgtagaggct gtttctgcac ctgcagcaat cacgtcagca atagcttctg
     3301 ggttacggtc agcaaccacc agcgagtaac ctgctttcag aaggttttta ctcattggtt
     3361 tacccataat ccccaggcca ataaaaccaa ctttcatagt catatcaatc atctctcttg
     3421 ttgcggtggt ggttattttt taaaggtatc agccagttta tgagtggcag agcggaagac
     3481 gccgagatcg ctgccgacag ccacaaacgt cgcgccccat tccagataac gacgcgcatc
     3541 ggcttcgacc ggcgcgagga taccgctggg tttgccgtgc gcgctggcac ggttaaaaat
     3601 gtgctgaatt gctttttgta catccgggtg tgatgcattg ccgagatggc ctaatgccgc
     3661 ggccagatcg ctggggccga cgaagatgcc gtctacgcct tcggtagcgg caatggcatc
     3721 gacgttatct acgccctgct gactttctat ctggaccaga atagtgatgt tcttgttcga
     3781 ctgagcgaaa taatccgcca cggtgccaaa catattggcg cggtgagaaa cggagacgcc
     3841 gcgaatgcct tccggtgggt aacgggttga tgccaccgcc tgctctgctt cctcttttgt
     3901 ttctacaaaa ggaatcagga agttatagaa accgatatcc agaagacgct taataatttc
     3961 cggctcgttg gtcggcactc acactactgg cgcgctggcg ctgcctttca aggccattaa
     4021 ctgcggaata aacgtggaga tatcgtttgg cgcatgttcg ccatccagca ccagccagtc
     4081 aaacccagcc aaaccaagaa cttcagtgct aatcgggtta gagagtgctg accagcaacc
     4141 aatttgtacc tgtttcgcag ccagtgcggc tttgaattta ttcgggaaaa catcgttatt
     4201 catcgcttat acctttgctt atttctgcaa ttccatacgt ttaatgtcgc caactacgaa
     4261 gaggtagcag accatcgcca tcagcgctga acatcccacg aaaaccagtg ctgcattgaa
     4321 ggagtgcagt tcacttacca ggtagccaat caccagtgga gtgacaatgg aggcaacatt
     4381 gccaaagacg ttaaagacgc cgccacagag gccaacaatc tctttcggcg cggtgtcaga
     4441 aatcaccggc cagcccagag caccaaatcc tttgccaaaa aaagccagcg ccatcagcat
     4501 gaccaccagc gtggtgttgt tggtgtagtt acataagatg atggtggaag ccagcaacat
     4561 tcccagcaca atcggtagct tacgtgccag ggtcagggat aaaccgcgtt tgatcagata
     4621 atccgagaag acacctccca gcacgccgcc cgcaaaacca cacagtgccg gaatcgaggc
     4681 gaccagaccc actttcagaa tcgacatgcc tttttcctgc accagataaa tcgggaacca
     4741 ggtgaggaag aaccaggtga tggtgttgat aaaatattgt ccgaaaaata cgcccaacat
     4801 catgcggtta gagagcaatt gcttgatgta atgcagtttg ggaccgcttg ctgccgcgct
     4861 gcccggcttt ttgtggtcca tatcgaccac cgcgccattt tcagagataa acttcagctc
     4921 ttcctcagac atacgtgggt gatctgtcgg gttatgaatc aacttgatcc acagcgccgt
     4981 cagcacaaaa caaatcaccc ccataacggt aaagacgtgc tcccatcccc aggcgaaagt
     5041 cagccagcca agcagaggcg aaaagagcgc cagcgagaaa tattgtgccg agttaaagat
     5101 ggcggaggca gtaccacgtt ctttcgtcgg gaaccaggcg gcgacaatac gggcgttcgc
     5161 cgggaaggat ggagcttccg agaatcccag cataaagcgc ataaagaaca tggagatccc
     5221 tgcccaagcc agcgggaaca tatcaacaaa gccttgcagg aaggtgaaca gcgaccagaa
     5281 aaagaggctg taggtgtaaa cttttttcga gccaaactta tcaagcaacc agccgccggg
     5341 gatttgcatc agcaagtagg cccagccaaa agcggagaag atgtaaccca tcgaaaccgc
     5401 acttaactgc aactcttttg ccacttcggt accagcaata gacagcgttg cacgatcggc
     5461 gtagttaacg gcggtaacaa taaaaataat cagtaatatt aaatagcggg tatgcacacc
     5521 tttccttttt tcgtcaacgg tgtccagaat cattttattt acctcgggta cttatgctga
     5581 tttttattat tatggggaag gtgttattta taagtttcat ttatgccgta acgacaatga
     5641 actcgggaat tagtataagc agcgcgagaa taataatcat tgtgcaaatg ctaatttaat
     5701 taatactttt taaatattat tttgagcata tgcacataag gttgcgcgct aaagcacaga
     5761 tttgcgcttt accttgccgg gcggcactgc aatccctaaa atgattgaca ttgatcacat
     5821 ttctgcgttt aaactcctga cattcttatt tcacccaatg aaatcattta tttttaaatg
     5881 agaccaggtc ctcattttaa taacccctgg ctggagaata ttgcacaatg gccaacatcg
     5941 aaatcagaca agaaacgcca actgcgtttt atataaaagt tcacgacaca gataatgtgg
     6001 caattattgt taatgataat ggcctgaaag caggaacgcg ttttccggat gggctggaat
     6061 taattgaaca tattccccag gggcataaag tcgcattgct ggacattccg gctaatggtg
     6121 aaattattcg ttatggcgaa gtgattggtt acgccgtgcg tgcaatccca cgcggaagct
     6181 ggatcgacga atcaatggtt gtactaccgg aagcgccgcc gttacacacg ctgccactgg
     6241 caaccaaagt cccggaaccc ttaccgccgc tggaaggata cacctttgag ggctatcgca
     6301 atgccgatgg cagcgtgggc accaaaaacc tgctcggcat caccaccagc gtccactgtg
     6361 tggcaggcgt ggtggactat gtagtaaaaa tcattgaacg cgatctgcta ccgaaatacc
     6421 cgaacgtcga tggcgtggtg gggctgaatc atttgtacgg ttgtggcgtg gcgattaacg
     6481 caccggcagc agttgtacct attcgtacca ttcacaatat ttcgctgaac cctaactttg
     6541 gcggcgaagt aatggtgatt ggcctgggtt gtgaaaagtt gcagcctgag cgcctgctga
     6601 ctggaacgga tgatgtgcaa gctattccag tagaaagcgc cagcattgtc agtttgcagg
     6661 atgaaaagca tgtcggtttt cagtccatgg tcgaggatat tttgcaggtc gccgaacgcc
     6721 atctacaaaa actgaatcaa cgtcagcgag aaacctgtcc ggcttcagaa ctggttgtcg
     6781 gcatgcattg cggtggcagc gatgcgtttt ctggcgtaac ggcaaacccg gcggttggct
     6841 atgcgtctga tctactggtg cgctgcggcg caacggtgat gttttcagaa gtcacggaag
     6901 tgcgtgacgc gatccatctg ctgacaccac gcgcagtgaa cgaagaggtc ggcaaacggc
     6961 tgctggagga gatggagtgg tacgataact atctcaatat gggaaaaacc gaccgcagcg
     7021 ccaacccttc gccgggcaac aagaaaggcg gtctagcaaa cgtggtagag aaagcgctcg
     7081 gctccattgc taaatcgggc aaaagtgcaa ttgttgaagt gctgtcgccc ggtcaacgcc
     7141 cgactaaacg cggattaatt tacgccgcga cgccagccag cgattttgtc tgtggcacgc
     7201 aacaggtggc ttcgggtatc accgtgcaag tgtttacgac cggtcgtggt acgccgtacg
     7261 gcctgatggc ggtacccgtc attaaaatgg caccccgcac cgagctggcg aaccgctggt
     7321 ttgatttaat ggatattaat gcgggcacca tcgctaccgg cgaagaaact attgaagagg
     7381 agggctggaa gttgttccac tttattctcg acgtcgccag cgggaagaag aaaaccttct
     7441 cggatcaatg gggactgcat aaccagctgg cggtgtttaa cccagcaccg gtgacctgat
     7501 aattcaattc gtattatgcc cgatagcaca cgtttatcgg gcatattgag tcacagcgaa
     7561 gacgcgtgat ccccgaccag aatcacttca accccagcct ttcgcagtcc ttccagacta
     7621 tccgcaggaa tgccttcatc aacaatgatc atgtcgatac gttgagtatc aatgatctta
     7681 tgtaaactgg aacgattgaa tttactggaa tcggtgacca cgatgatccg ttccgcaact
     7741 tcgcacatcc gacggcttaa acgggcttca tcttcattat gtgtgctgac gccgcgctcc
     7801 agatcgatcg catctacacc aagaaacagc atatcgaagt ggtaattttg cagcgattgc
     7861 tcagcctgat cgccgtaaaa agattgcgac tgacggcgca aatgcccgcc ggtcatcagc
     7921 agctcaacgc cttccgcttc cagcaacgca ttagccacgt tcataccgtt ggtcatcgca
     7981 attacgtcag tgtacttgcg catcagacga gcaatctcaa aagtggtggt cccggaatcg
     8041 aggatcaccc gatgacctgg ctgaatcaac tcaacggcag ctttcgcaac gctgcgtttc
     8101 atcgcggtgt tcagtgcgct tttatcttcc actgatggct cgactgacgg cgtcgtgcta
     8161 tcgcagatca acgcgccacc ataggcacgc acagcgatcc cctgcttttc cagaaacgcc
     8221 agatcattgc ggatcgtcac agtagatacg ccatacaatg ccgacagatc gttaacctgc
     8281 acactccctt gctgtcgcag acgctgaatg atctgttctc gtcgctcgct ggtgcctgtc
     8341 actcgcttct cacctgaagc gtcggtatta ctcatagtaa gtcctttcgt aaaactttcg
     8401 tttcatttcg ttttgcctat taacgccttt ctattaagca aatgcaagcc caccttgccc
     8461 attggcgcaa gctactctcg tttcagtgac tttcattacg tttcttttgt gaatcagatc
     8521 agaaaactat tatctttcgt tttattttta tctcaccatg acgcagtatc aattgaaaca
     8581 aaacgaaaga tcaatatcgc agtaatctga actggagagg aaagtgaaac atctgacaga
     8641 aatggtgaga cagcacaaag cgggcaaaac aaatgcaatt tatgccgttt gttccgcaca
     8701 tccgctggtg ctggaggctg caatccgcta cgccagtgca aaccaaacgc cgttactgat
     8761 tgaagcaacc tccaatcagg tagaccagtt cggcggttat accggaatga cgcccgccga
     8821 ttttcgcggc tttgtttgtc agctcgccga ctcgttgaat ttcccgcagg atgcgctgat
     8881 tctgggtggt gaccatctgg ggccaaaccg ctggcaaaac ctgccagccg ctcaggcaat
     8941 ggccaatgcc gatgatttga ttaaaagcta cgttgcggca ggattcaaaa aaatccacct
     9001 tgattgcagc atgtcctgtc aggacgatcc aattccctta actgatgaca tcgtggctga
     9061 acgcgccgcc cgtctggcga aagtggcgga agaaacctgt cgcgaacact ttggcgaagc
     9121 cgatctggag tatgtcattg gtaccgaagt gccggtacct ggcggcgcgc atgaaacctt
     9181 aagcgagctg gcggtcacca cgccggatgc cgcccgcgcc acgctggaag cccatcgtca
     9241 cgcctttgaa aagcaaggtt tgaatgccat ctggccacgc attattgccc tggtggttca
     9301 acccggcgtc gaattcgatc acaccaacgt tattgattat cagcccgcca aagcgagcgc
     9361 cttaagccag atggtcgaaa actacgaaac gctgattttc gaagcgcact ctaccgatta
     9421 tcaaacgccg caatctctgc gccagctggt gattgaccac tttgccattc tgaaagttgg
     9481 cccggcgctg accttcgccc tgcgtgaagc ccttttttct ctggcggcga ttgaagaaga
     9541 actggtacca gcgaaagcct gttccggtct gcgtcaggtg ctggaaaacg tgatgctcga
     9601 ccgcccggaa tactggcaaa gccactacca cggtgacggc aacgcgcgtc gtctggcgcg
     9661 tggttacagc tactcagatc gcgtgcgcta ttactggccg gacagccaga ttgatgacgc
     9721 tttcgctcat ctggttcgta atctggcgga ttcaccaatt ccgctaccgc tcatcagcca
     9781 gtatctgccg ctgcagtacg tgaaagttcg ctccggcgag ctgcaaccga cgccacggga
     9841 actcattata aaccatattc aggacatcct ggcgcagtac cacacagcct gtgaaggcca
     9901 ataagcaaaa caaagaggaa catgctatgc caaatattgt tttaagccgg attgatgaac
     9961 gcttgattca cggtcaggtc ggcgttcagt gggtcggatt tgcgggggca aatctggtgc
    10021 tggtagccaa cgatgaggtt gccgaagatc cggtacaaca aaacctgatg gaaatggtac
    10081 tggcagaagg gatcgccgta cgtttctgga cgctgcaaaa agttatcgac aacattcatc
    10141 gcgccgccga tcgacagaaa atcctgctgg tttgtaaaac acccgccgat ttcctgacgc
    10201 tggtgaaagg tggcgttccg gtgaatcgca ttaacgttgg caatatgcac tacgccaatg
    10261 gcaaacaaca aatcgccaaa acggtttctg tggatgcggg cgatatcgca gcatttaacg
    10321 acctgaaagc cgctggggtg gaatgcttcg ttcagggcgt cccgacagag cctgctgtgg
    10381 acctctttaa attactttga gggattcatc atggaaatca gcctgttgca ggcatttgcg
    10441 ttgggcatta tcgcctttat cgctggccta gatatgttta acggcttaac ccatatgcac
    10501 cgcccggtgg tcctcggccc gttggtcggg ctggtacttg gcgatctgca taccggaatt
    10561 ttaaccggcg atacgctgga actagtgtgg atggggctgg ccccgctggc gggcgcacag
    10621 ccgcctaacg tgattatcgg tactatcgtc ggcacggcgt ttgccattac tactggcgtg
    10681 aaacccgatg tcgcggtcgg tgtcgccgta cctttcgctg tcgcagtaca gatggggatt
    10741 accttcctgt tctcggtgat gtctggcgtg atgtctcgct gcgaccggat ggcggagaac
    10801 gccgatactc gcggcattga acgcgtgaac tatctggcac tgctggcact cggcactttc
    10861 tattttctct gcgctttcct gcctatctac ttcggcgcgg aacatgcaaa aaccatcatt
    10921 gatgtcctgc cgcaacgatt aatcgacggc ctcggcgtcg cgggcggcat catgccagca
    10981 atcggttttg ccgtgctgct gaaaatcatg atgaaaaacg tctacatacc ctacttcatc
    11041 ctgggctttg ttgccgcaac ctggctcaag ttaccggtgc tggctattgc tgccgccgcg
    11101 ctggcgatgg cgctgatcga tctgctgcgt aaatctcctg aaccgactca acctgcggca
    11161 cagaaagagg aatttgaaga tggcatctaa tcaaaccacc ctgccgaacg tcagcgaaaa
    11221 tgaagaaaca ctgctgactg gcgtcaatga aaacgtgtat gaagatcgga gcattgccgc
    11281 ggagctgacg aaaaaagata tcaaccgcgt cgcctggcgt tccatgctgt tacaagcgtc
    11341 ttttaactac gaacgtatgc aggcttctgg ctggttgtac ggtctgctgc ctgctctgaa
    11401 aaagatccac actaataaac gcgacctggc gcgcgccatg aaggggcata tgggtttctt
    11461 caatacccat ccgtttctgg tgacgtttgt tatcggcatt atccttgcga tggagcgttc
    11521 taagcaggac gttaacagta ttcagagcac caaaattgcc gtcggtgcgc cgcttggcgg
    11581 gattggcgat gcgatgttct ggctaacgct actgccgatt tgtggcggga taggcgcaag
    11641 cctcgcgcta caaggctcta ttcttggcgc agtcgtcttt attgtgctgt tcaacgtggt
    11701 acacttgggg ctgcgttttg gtctggcgca ttatgcttac cgcatgggcg tggcggcgat
    11761 tccccttatt aaagcgaata ccaaaaaagt cggccatgct gcgtcaatcg ttggaatgac
    11821 ggtaatcggc gcactggtgg caacctatgt tcgtttaagc accacgctgg aaatcacggc
    11881 gggcgacgca gtagttaagt tacaggctga tgttatcgac aaactgatgc ctgccttctt
    11941 accgctggtc tacaccctga ccatgttctg gctggtacgc cgcggctaga gtccgctgcg
    12001 cctgattgcg gttaccgtgg ttctagggat cgtcggtaaa ttctgccatt tcctttaaat
    12061 acaatgaggt attagatgtt aagtattatt ttgacagggc atggcgggtt tgccagtggc
    12121 atggaaaaag caatgaagca aatcctcggc gatcaatcgc agtttattgc catcgatttt
    12181 cccgaaacct caagcgccgc gctgcttaca tcgcagcttg aagaggccat cgcccaactg
    12241 gattgtgaag acggcattgt ttttctgacg gatttgctgg gcggcacgcc gtttcgcgta
    12301 gcttcaacgc tggcgatgca aaaaccgggc tgtgaagtga ttaccggcac caatttgcaa
    12361 ctgctactgg agatggtgct ggagcgcgaa gggttaagcg gtgaagaatt ccgtgtacag
    12421 gcgctggagt gtggacaccg agggctgacc agtctggtgg acgagttagg ccgctgccat
    12481 gaagaatgtc cggtcgagga aggaatatga cacacgttct gcgcgccaga aggctgctga
    12541 ctgaagaggg atggttcgat gaccatcagt tgcgtgttgc tgacggtgtc atcgcagcaa
    12601 tcgaaccgat tccagtgggc gtgactgaac gcgatgcgga acggctctgc cccgcttaca
    12661 tcgacaccca tgtacacggt ggtgcgggcg ttgatgtaat ggatgacgcg ccggatgtac
    12721 tcgacaagct ggcaatgcac aaggcacgcg aaggtgtcgg cagttggtta ccgaccaccg
    12781 taaccgcgcc gcttaatacc attcatgcgg cgctgaaacg tattgctcaa cgttgccaac
    12841 gcggcggacc tggtgcgcaa gtgctgggga gttatctcga aggaccgtac ttcacgccgc
    12901 agaataaagg cgcgcatccg
//
LOCUS       DAZXUL010000113        12733 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZXUL010000113 DAZXUL010000000
VERSION     DAZXUL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.113"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1038
                     /locus_tag="ACH3L1_003298"
     CDS             <1..1038
                     /locus_tag="ACH3L1_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFT4077924.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1039..3204
                     /locus_tag="ACH3L1_003299"
     CDS             1039..3204
                     /locus_tag="ACH3L1_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFT4077925.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3218..4129
                     /locus_tag="ACH3L1_003300"
     CDS             3218..4129
                     /locus_tag="ACH3L1_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFT4077926.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4129..5424
                     /locus_tag="ACH3L1_003301"
     CDS             4129..5424
                     /locus_tag="ACH3L1_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFT4077927.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5469..5699
                     /locus_tag="ACH3L1_003302"
     CDS             5469..5699
                     /locus_tag="ACH3L1_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077928.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5677..6177
                     /locus_tag="ACH3L1_003303"
     CDS             5677..6177
                     /locus_tag="ACH3L1_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFT4077929.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6177..7595
                     /locus_tag="ACH3L1_003304"
     CDS             6177..7595
                     /locus_tag="ACH3L1_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFT4077930.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7595..8443
                     /locus_tag="ACH3L1_003305"
     CDS             7595..8443
                     /locus_tag="ACH3L1_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFT4077931.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8443..8898
                     /locus_tag="ACH3L1_003306"
     CDS             8443..8898
                     /locus_tag="ACH3L1_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFT4077932.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8901..9593
                     /locus_tag="ACH3L1_003307"
     CDS             8901..9593
                     /locus_tag="ACH3L1_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFT4077933.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9603..10934
                     /locus_tag="ACH3L1_003308"
     CDS             9603..10934
                     /locus_tag="ACH3L1_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFT4077934.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10935..12665
                     /locus_tag="ACH3L1_003309"
     CDS             10935..12665
                     /locus_tag="ACH3L1_003309"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFT4077935.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         3435 a         3143 c         3355 g         2800 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgcagact ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt
     1141 agccgagtgt cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga
     1201 caattcgatg ttgttcgccc ggaggtcagg aaactggtcg cagagatgcg ccggaaccct
     1261 gtcgacgttc tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg
     1321 gggatgtatc gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt
     1381 cgtgagcaga tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac
     1441 gatccaacaa gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac
     1501 gtcatatggg acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg
     1561 attaacgcct tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg
     1621 gataccctgc catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat
     1681 gatgtcgtct acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc
     1741 taccgcgacc cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc
     1801 atcgacgacc tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg
     1861 cgtgtgtata agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc
     1921 ggagagcata ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag
     1981 tgctacgagg gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg
     2041 tcgttcaacg ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag
     2101 caaatcgaag gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag
     2161 aacaggactg atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac
     2221 cctgaagtgc cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa
     2281 gtggctagtc ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc
     2341 aatcaactga acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc
     2401 gcaatgcgac gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct
     2461 cgtcatgtaa cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa
     2521 gttgtcgatt accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag
     2581 tgctatacag acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt
     2641 caggagttgc tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa
     2701 tacttcacgc tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa
     2761 ctggtgatga tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag
     2821 gcacaacaac agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg
     2881 caaggtcagg ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct
     2941 gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat
     3001 atggacctcg acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag
     3061 caacagcgca gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga tgcagaccag
     3121 actcattcac aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc
     3181 ggagtagccg agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca
     3241 ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt
     3301 tgtcgataat gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tccggaaaga
     3361 cgatgagaaa ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga
     3421 acgcaaacgc cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc
     3481 ggagcacctg cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc
     3541 cgaagatgca ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca
     3601 ggcaaacagt gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca
     3661 gggtcgcaaa actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa
     3721 gcactacgag gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt
     3781 catgcaactg gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc
     3841 cgccgctctc atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat
     3901 ggacgggcaa tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc
     3961 tcgagccaag cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt
     4021 tgctgcaaat atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt
     4081 agagacgtac cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat
     4141 gaaggtcaac tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg
     4201 ccaatggcgt caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc
     4261 aacaccgtgt ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact
     4321 ggcaacgcaa ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac
     4381 gatttcttcg agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc
     4441 caggcatccg ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact
     4501 gaaatgggct cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct
     4561 ggctgggatt ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac
     4621 atgggcatca gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta
     4681 gatggtgaca tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt
     4741 cagattgctg gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca
     4801 accgcgactg gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt
     4861 gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc
     4921 accaccggat ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag
     4981 atggcgaaga atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt
     5041 actcacatcg aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa
     5101 gagaaggctt acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg
     5161 aacgtggcaa caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct
     5221 cagccgatcc cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt
     5281 ccaggtattg gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt
     5341 aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt
     5401 ggtctgccta accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg
     5461 agacaagcat gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg
     5521 gctacgactt tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt
     5581 tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc
     5641 gtaaaaagcc tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat
     5701 ttagttcttg cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc
     5761 gaaccgcagt ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt
     5821 ggcggtgatg cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct
     5881 ccggatccgg gcgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac
     5941 cttgcctgcc gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tataaccact
     6001 gccagatacg ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct
     6061 aaatgtaagt ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag
     6121 tggaatggtt ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc
     6181 ctattcagca acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata
     6241 tcgactatct gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat
     6301 atcttcgctc attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg
     6361 tcgagtacaa catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag
     6421 gagaaagtga agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga
     6481 catcacaggc ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca
     6541 aaaccgtctc aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc
     6601 gcgacattac gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt
     6661 ttatcactga ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg
     6721 agtctcagcc tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg
     6781 gttcatcgac tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt
     6841 atgttgcaca accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc
     6901 cattcgctga ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata
     6961 tcatcgactc cggtcaggtg tcaccaattg ccagcgcgag cattgagaaa atcctccgct
     7021 cctacactgc tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg
     7081 agttgctgat tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaatcgcca
     7141 atggcccaca atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg
     7201 acttcgttta cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga
     7261 aactgcaatt cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc
     7321 cactcttcaa agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg
     7381 ttgcgcagta cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc
     7441 gtgagcagat gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc
     7501 gagtagggcg catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg
     7561 tcactctgtc tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc
     7621 ggtaatcatc caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca
     7681 gtcgtatctg ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc
     7741 caacgaggcc ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat
     7801 tctcgctgac catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga
     7861 gcgaatcaga caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga
     7921 aagcgatatt tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca
     7981 tgaaacgcgc atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta
     8041 ctctggtgta tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac
     8101 gctcacaccg tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt
     8161 tgtgttcaac gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag
     8221 cggaaccgcc aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt
     8281 cagcagtcgt aatgcggcga caacaaccga caacatcctg ttagctacgt tcttcagcgt
     8341 ggataaagac ggctttcttg ccacaaatgg cagtacgtta accattcagt cgaatggtgc
     8401 ggcgtttact gccacaacca tcaagataat cgcggagcag tgatgattac attcaaacca
     8461 acgcgaaaca tcgacctgat agaagcagtc ggaaatcacc ctgacattat cgccgggagc
     8521 aacaacggtg atggatacga ctacaaacct gaatgccgtt actttgaggt gaacgtgcac
     8581 ggtcagtttg gcggcattgt ttactatcag gagattcagc cgctgacatt agattgccac
     8641 gccatgtacc tgccagagat tcgcggcttc agcaaggaaa ttgggctggc gttctggcga
     8701 tacattctga ctaacaccac cgttcagtgc gtaacatcgt tcgctgcacg caaattccga
     8761 cacggtcaga tgtactgcgc aatgattggc cttaagcgtg taggaaccat caagaaatac
     8821 ttcaaaggcg tggatgacgt gactttttac agcgccacac gcgaagaact aatcgacttc
     8881 ctgaatcacg ggagatagcc atgttatatg catttaagct gggcagaaaa ctgcgcggcg
     8941 aggaacctta ttgccctgaa aaaggcggga aaggtggcag ttctgataaa agcgcaaagt
     9001 atgcagcaga agcccagaag tatgctgcag acctgcaaaa tcagcagtgg cagacgatca
     9061 tgaaaaacct tgctccgttc acgcctcttg cggagcagta tgttaaccag cttcagaacc
     9121 tttccagttt agaaggtcag gggcaggcac ttaatcagta ttacaactct aagcagtata
     9181 aagaccttgc agggcaggcg cgttaccaga gtcttgctgc tgcggaggcg acgggtggac
     9241 ttggttcgac agccacaagc aatcaactgg ctacgatcgc gccgacactc ggtcagtctt
     9301 ggttatcaaa tcagatgagc aattacaaca atctggcaaa cgttgggctt ggtgcgctgc
     9361 aaggtcaggc aaacgccggg cagacgtacg ccaacaacat gagcagcatt gcacagcaaa
     9421 gcgcagctct tgccgctgct aatgccaata aaccatcaag tcttcagact gcaattagcg
     9481 gtggcacgtc tggtgcgatt gccggtgcag gtcttgccag ccttttggga acatcaacgc
     9541 cttggggcgc tggtattggt gctggtatcg gattgcttgg ctcgttgttt taagggggaa
     9601 tcatggctac ttggcaagga acaaacggcg gattgttggc tggtatcggc ggcgtcaact
     9661 caaacgctcc gagcgtaaat gacatcggca atacgcttca gcttatcagg cagaacaatg
     9721 atattgagcg ttcaggcgct aacaatgttg ggctgactgc tttgcaaggt ctttcaggta
     9781 ttgcgggtgt ttttcagcag gaaaagcagg ctcagcggca gaaagaattt cagcaggcgt
     9841 acgctaatgc ttatgcgtct ggtgatcgcg gtgctttgcg tcagttggct actcaatatc
     9901 cagaccagat tgaatccgtt cgtaaaggca tgggattcat tgatgaagag cagcgtaatt
     9961 ctatcggcac cttagcggct ggcgcacgcc ttgcgtcatc gtctccagaa gcaatgcaat
    10021 catggctgca aaacaacgcc ggtgagttag ctcgtgttgg cgttaatcct catgacgtcg
    10081 ctcagatgta ccaacagaac ccgcggcagt tcggcgaatt tgtcgatcac ctggggatga
    10141 acagtctcgg gcccgaaaaa tactttgacc tacaggataa aatgcagggt cgccaggtta
    10201 ccatgcgcgg tcaggatctg gattcgcaaa ccgccgctcg gaatcaggca atcacaatgc
    10261 gcggacaaga tatccaggcg aatttaggtc agcagcgcat taatctggac gcagaaacaa
    10321 accgtattaa caacgaaaat aagcgccttg accggatgct atcagcagaa actaacgacc
    10381 tgaagcgcca ggaaatacag agccgcatag cagccaacaa ccagcagttg cagcagaagc
    10441 agcaagcgct aaatgatggc tacaaagacg gcatcaacac cctcacaacc agcatgttca
    10501 ctctgaacga tatcgttagt tctccttcac ttaagagcat tacaggctta cgtggagtaa
    10561 tccccaacgt tccaggctca caggctgcag acactcaggc acgacttgat acctttaaat
    10621 cacaagcata cctgacagcg gttcaggcca tgcgaggcat gggcgcactt tctgatgccg
    10681 agggcaaaaa gctcgaccag gctgttggtt cgctgcagaa ctcgcagagc gaggagtcct
    10741 ttcgtcgcaa cgctggcgtc atcctgaaca cgctcaacca gaagcgtaat gaggcggttg
    10801 gtaagtacgt tcagcaaaac ggtatcaagc gagtggaagc gcctcaggct tctatagatt
    10861 acctgaagca gcaccccgag ctgtcaatcg acttcattaa tcgctacgga tatcttccat
    10921 ctttggggca gtaaatggct aattaccgtg atttgttaga gcaggctggc gcacgttacg
    10981 gtgtgccaga agggttgatg actgcactgg gtgccaagga gtcttcttac aaccctgccg
    11041 cagtaagctc cgccggggct gtaggattga ctcaggtcat gcctgggaca tggcgtgata
    11101 tgggttatac cgatgagcaa atgcaaaacc ccgaatatca ggctgacgct ggcgcgcgct
    11161 atctggcaaa gatgtaccag cagtttggta actggcgtga ctctcttcag gcttatcacg
    11221 acggtcccgg caacgttatg aaggcaaagc gtggtgaata tacgccagga cctgaaggcc
    11281 gcggttacgt tgatgatcgc tttgctcaat gggctggtga cccggtgaca gactcaacag
    11341 tcgaacagcg cgccacctct gcaaaggtac atcctcagca agaccctaac aacccgtttg
    11401 cacaactgga agtacagtca tccgaacaag tatcggcatc aggtgtgcag tcagacccaa
    11461 ataatccatt tgctcagatt gagcagcagg cagccagtca gcagccacct caacccgtaa
    11521 gttctgtcgc accgaaacct gttcagcaac aaacagttaa tcaggccaat aatgcaccaa
    11581 cacgggaaga accatcattg atgcaacaag ctggcgattg gctcactggt ggtcaaagtg
    11641 cagggcaaat tgcagaacag gctggtcgtg gtctggtaaa cataccattt gacgtattgc
    11701 agggtggcgc aagtctgatt aatgcaatca gtcaggggct tggggggcca aaagtattgg
    11761 atgatgttta tcgtccagta gacagaccga cagaccccta cgcgcaagct ggagagtcaa
    11821 taggcggtta tcttgttcct ggagcaggtg tagctggaaa catggtcatt ggttctctcg
    11881 ctgacgcggc gaatcaacgg ggtgattttg ccgaaaatgc cgctattaat gccggactta
    11941 gcattgctac gcatggcctg ataaatggcg ttacccgtgg tgttcgtggt gcatcaaata
    12001 taattagtgg caataaaaca tctgcacaga gagcgaccac tgcgccaaca gaaacatcac
    12061 cattctccgg tgatgccgct gcagcaacaa atcctgcggt tcatgccgca gaggcaagag
    12121 tagcacaagg tgtaccaatg acgcctgcga cgaagaaccc agaggaagtc gttcgcacag
    12181 tagcagcaca aaaaaggcca aatctcgctt catcgcttga tgaactagat atcaatcctc
    12241 aggctgaagt tctggagtct gctgaaaggc ttaatgttga ttcattactc ccttcacact
    12301 tttccgggaa cgagcaatac aaggcagttg agcaagcaat caagtcccgt gcgggttctg
    12361 ctctacaggt gcaggaaaat gaagcaatca ggcagctagc acagggcgcg ggtgagataa
    12421 ttgatcgcgt ttccggtgca aaagatgctc ttggtatgag cgacaagttt attgatacgg
    12481 tcaatggaag aatgtctgcg ctgatgaaac gaagcgacca gctttatcgc aatgttgaaa
    12541 aggcgatgcc tgcaggtgca aaaattgatg cgccatcaac aaggtcaatg ctcaaacagg
    12601 tggcagaaga tcttggcggg atgaaaaacc ttgaccctat tgaaaagaga gttgaggtag
    12661 cctgagttta acggacactc cttcctgaaa tagaatggca tcagaaggag ctaataatga
    12721 gcagaaaaac cca
//
LOCUS       DAZXUL010000114        12499 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZXUL010000114 DAZXUL010000000
VERSION     DAZXUL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12499
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.114"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="ACH3L1_003310"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="ACH3L1_003310"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFT4077936.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="ACH3L1_003311"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="ACH3L1_003311"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFT4077937.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="ACH3L1_003312"
     CDS             3115..3399
                     /locus_tag="ACH3L1_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFT4077938.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACH3L1_003313"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACH3L1_003313"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFT4077939.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACH3L1_003314"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACH3L1_003314"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFT4077940.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACH3L1_003315"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACH3L1_003315"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFT4077941.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="ACH3L1_003316"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="ACH3L1_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFT4077942.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9585..10685
                     /gene="ompC"
                     /locus_tag="ACH3L1_003317"
     CDS             9585..10685
                     /gene="ompC"
                     /locus_tag="ACH3L1_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFT4077943.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10849..11334
                     /locus_tag="ACH3L1_003318"
     CDS             10849..11334
                     /locus_tag="ACH3L1_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFT4077944.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11444..12499
                     /locus_tag="ACH3L1_003319"
                     /pseudo
     CDS             11444..12499
                     /locus_tag="ACH3L1_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT         3278 a         3099 c         2914 g         3208 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt
     9481 tttaatcacc attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt
     9541 cgtttagaaa atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag
     9601 ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata
     9661 acaaggatgg taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg
     9721 atgataagcg tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc
     9781 aaatcaatga tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg
     9841 atgaagccaa cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta
     9901 gtgaatttgg ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat
     9961 ggaccgatat gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga
    10021 cataccgtac caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag
    10081 ggctgaactt tgccgcgcaa tatcaaggca aaaataaacg tactgacaac agtcatcttt
    10141 atggtgctga cgacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt
    10201 atgatggctt tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa
    10261 gagccgcagc taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta
    10321 taaagtatga tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga
    10381 ccacctatgg ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat
    10441 atcaattcga cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc
    10501 tgggccgcta cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct
    10561 tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact
    10621 ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt
    10681 tctaacgtcc tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt
    10741 atgactcctt aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat
    10801 attattccat atcctccggg gacatcttac tataatattt attgatgagt gattatgacc
    10861 aatattaata cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct
    10921 aacaaagaaa ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg
    10981 gttaataatg gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg
    11041 aacatggcat ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg
    11101 acagttaata cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat
    11161 gccagtctcg ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa
    11221 gaaccaaaac tggaaaacat ttatattaat cataacaatc tggaaagttt agatttaaaa
    11281 aatcagcatt cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc
    11341 tgatagttat aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt
    11401 ctcccggcaa gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat
    11461 attacacaat catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt
    11521 aaacgccaaa aacactacaa aactggaata tttaaactta agcaataaca atttactgcc
    11581 aaccaatgac attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg
    11641 catcaacaat gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga
    11701 tgacactaat gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg
    11761 gctgcaaaac ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac
    11821 caactacgat agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt
    11881 tcctcaaggc agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa
    11941 tattgatata gataggcttt catcagttac ttattttagt gcggcacata atcaactaga
    12001 gtttgtgcaa ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt
    12061 aactgatatt gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa
    12121 actaacaagt ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa
    12181 tttgctttct gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc
    12241 tgctaacaac cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa
    12301 aagtttaaga ctggacaata ataaaataac ccacattgat actaataata catccgatat
    12361 tggaacttta ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg
    12421 gaagaacaat tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa
    12481 gcttaatact ggcctggca
//
LOCUS       DAZXUL010000115        12322 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZXUL010000115 DAZXUL010000000
VERSION     DAZXUL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12322
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.115"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            34..435
                     /gene="gpFI"
                     /locus_tag="ACH3L1_003320"
     CDS             34..435
                     /gene="gpFI"
                     /locus_tag="ACH3L1_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFT4077945.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="ACH3L1_003321"
     CDS             447..818
                     /locus_tag="ACH3L1_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFT4077946.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..1395
                     /locus_tag="ACH3L1_003322"
     CDS             805..1395
                     /locus_tag="ACH3L1_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFT4077947.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            1392..1793
                     /locus_tag="ACH3L1_003323"
     CDS             1392..1793
                     /locus_tag="ACH3L1_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFT4077948.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            1804..2544
                     /locus_tag="ACH3L1_003324"
     CDS             1804..2544
                     /locus_tag="ACH3L1_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFT4077949.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2603..2989
                     /locus_tag="ACH3L1_003325"
     CDS             2603..2989
                     /locus_tag="ACH3L1_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFT4077950.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2998..3327
                     /locus_tag="ACH3L1_003326"
     CDS             2998..3327
                     /locus_tag="ACH3L1_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFT4077951.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3299..6340
                     /locus_tag="ACH3L1_003327"
     CDS             3299..6340
                     /locus_tag="ACH3L1_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFT4077952.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            6340..6669
                     /locus_tag="ACH3L1_003328"
     CDS             6340..6669
                     /locus_tag="ACH3L1_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFT4077953.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6669..7367
                     /locus_tag="ACH3L1_003329"
     CDS             6669..7367
                     /locus_tag="ACH3L1_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFT4077954.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            7372..8115
                     /locus_tag="ACH3L1_003330"
     CDS             7372..8115
                     /locus_tag="ACH3L1_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFT4077955.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            8079..8654
                     /locus_tag="ACH3L1_003331"
     CDS             8079..8654
                     /locus_tag="ACH3L1_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFT4077956.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            8715..12194
                     /locus_tag="ACH3L1_003332"
     CDS             8715..12194
                     /locus_tag="ACH3L1_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFT4077957.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            12262..>12322
                     /locus_tag="ACH3L1_003333"
     CDS             12262..>12322
                     /locus_tag="ACH3L1_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HFT4077958.1"
                     /translation="MRKLYAAILSAAICLAVSGA"
BASE COUNT         2909 a         2910 c         3923 g         2580 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atccgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccgctgtc cggaccgctg acacaggcat ttgaagatgc ccgcgaccgc
     1321 atcattgctg cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta
     1381 catctgagta aatgaaccga cacacacaaa tccgtcagag cgtgctggca cgccttcggg
     1441 aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcagg
     1501 aactgcctgc cgtggcggtg tggctgagtg atgctcagta caccggaaaa atgacggatg
     1561 aagatgactg gcaggctgtt ctgcatattg ccgtcttcat ccgggcacag gcaccggatt
     1621 cagagctgga tatgtggatg gagagcacca ttttcccggc cctgaatgat gtaccggcac
     1681 tttccggact catcgacacc ctgaacccac tcggttttaa ctatcaacgt gataatgaga
     1741 tggctacctg ggcgatggcg gaaatcacgt accagatcac gtacacgaat taaggaggtg
     1801 gtaatgacca caccaaatcc actggcaaag acgaaaggtg cggggacgac gttctggatg
     1861 tataccggca acggcgatgc gtttgcgaac cctttgtcgg acactgactg gctgcgtctt
     1921 gcgatggtga aggatctgca acctggcgaa atgaccgctg atgcagaaga tgacacttat
     1981 ctcgatgatg aagatgcaga ctggaaaacg acaacccagg ggcagaaatc cgttggtgat
     2041 acttcggcga cgctggcctg gcgtccgggt gacagcgggc agaaaaaact ggttcagttg
     2101 ttcgactccg gtgaagtctg cgcgtttcgt atcaaatatc ccaacggcac tgttgatgtt
     2161 ttccgcggct ggctgagctc actgggtaaa accattgcct caaaagacgt gatgacccgc
     2221 actgtgaaaa tcagcggtgt ggggcgtccg tatctggcag aggaaggcac tgaaaccgtg
     2281 ggtgttaccg gactgacggt ggcaccggca tctgtcagtg tcaaagcggg agcaaccacc
     2341 acactgacct ttacagtaaa acctgacggg gccagtgaca aagcgatcag tgtgcattcg
     2401 tcagatcctc agactgcttc ggtgaccctg agcgggcttg tggccacggt gaaaggcgtg
     2461 aagcagggca gtgtcagcat cgtgggcatg acctctgacg gagagtttgt ggcagtggct
     2521 gcggtgaccg tcagcgcacc ataacaggac gatactcatc attgccccgg ttatccgggg
     2581 ctttttgcat ccggagaaca tgatgtttct gaaacaggat acgtttaatt atgaaaaaca
     2641 gtccgtggtg ctcagtgagc tgtccgggct gcagagaatt gaatatctga cgtttgttca
     2701 gcagcgaacg gcaaagtttg atgcacagga gggagaactg ccggaggctg aacgacagat
     2761 tgcttttctg cgtatgggaa tggatatcaa tgcctggctg gtttcccgct cactgtggaa
     2821 tgctgagcag tctcaggatg ttgagacgct ttgcgcatcc attatgacaa catggtcgta
     2881 tgatgcgctg ggcgcggggg cggagagggt tctgtcgctg agcggtatgg ggaccattga
     2941 gaatgccggg gatgatgatc atgaggcgct gacgccggaa aagtcctgac gcgggaaatg
     3001 cagtttgtca tgcggcttgc ccgggagttc cggcgggcag actggcggcg gatgctgtcg
     3061 gatatgtcgg ccactgagct tggtgagtgg ggcgattatt tccggatgca gagcttcagt
     3121 gatgtgtgga tggatgcgca gtttgcctcg ctgaaggcat tgatcgtgag aatggtgtcc
     3181 ggcagcagcg atgctgcggt ggctgatttc agccttttac cggaagagaa cgggataccg
     3241 gagcgaacgg acgaagaact gatgcatctt ggggaaggta tttccggagg tgtgcgttat
     3301 ggaccagata gccaacctgg tcattgattt ggggattgat gcggcagagt ttaaaaatga
     3361 aattccccgt atcaaaaacc ttctgaatgg tgcagccagc gatgcagaac ggtcttctgc
     3421 ccgtatgcag cgttttatgg agcgtcagac tcaggcggcc cggcagacaa tgcaggcggc
     3481 ttcttcggct gcaacagcag catcagccca tgcgcagacg gtggagaaga atgcacgggc
     3541 tcatgaacgc atggcccgtg aggtggaaca aacccgtctg cgcgtggatg ccctgaatca
     3601 gaaaatgcgc gaggaacagg cgcaggccag ggcactggcg gaggcgcagg ataaagcggc
     3661 tgccgccttt tatcgccaga ttgacagtgt gaaacaggcc ggtgcggggc ttcaggaatt
     3721 acagcgtatt cagcagcaga tccgacaggc cagaaacagt ggcggggttg gtcagcagga
     3781 ttatctggcg ctgatttcgg agatcacggc gaaaacccgt gccctgacgc aggcagagga
     3841 acaggccacc cggcagaaag cggcgtttat ccgccagctt aaagagcagg caacccgcca
     3901 gaatctgtcg tcttctgagt tgcttcgcgc cagggcggct cagctggggg taagcagtgc
     3961 tgcagaagtg tatatccgca aaatggagcg ggcaggaaaa gccacacatt cgctggggct
     4021 gaaaagtgcg gcagcccggc gggagctggg ggtgttaatc agtcagatgg cgcgaggcaa
     4081 ttttggtgcg ctgaggggat ccgggataac gctggctaac cgtgccggat ggacaggcgc
     4141 actgatgtcg ccgaaaggca tgatgactgg cggcgttatt ggcggacttg tcgcggcggt
     4201 cctgggtctg ggtaaagcct ggcatgacgg ccggaaggag ggcgagaaat ttaaccgtca
     4261 gctggcgctg acgggacatt atgccggtgt cactgtcggg cagttgtgga aactcagccg
     4321 ggccatatcc gggaatggta tcacgcaaca tgcggcagcc ggtgcgctgg cacaggtggt
     4381 gggcagtggg gcatttcatg gaaacgatat tggtatggtg gcgaaagctg ccgcacagat
     4441 ggagcgatcg gttggccagt cggtcagcga taccataaat cagtttaagc ggctgaagga
     4501 tgatcctgta aatgccgcga aggcgctgga caatgcgctg cattttttga atgccactca
     4561 gcttgagcag atacgcgtcc ttggggagca ggggcggtcc agtgatgcgg cccggatcgc
     4621 catgtctgcg ctggcagagg aaaccggtaa acgcacatct gatattgata ataatctcaa
     4681 tgcgctgggt agtacgctgc aaaccttgtc tgactggtgg aagcagttct gggatgcggc
     4741 catgaatatt ggtcgtgaag actcgctgga tgcgcagatt gatgcgttac aggaaaaaat
     4801 tcagcgcgcg aaaaaatatc cgtggacaaa cgcctccaca caggtggagt acgatcagca
     4861 gcgtcttaac gatcttcagg agaaaaaacg ccggaaggat ttgcaggatg caaaagcgca
     4921 ggcagaacgg aattaccagg agcaacagaa acgccggaat gctgaaaatg ccgcgctgaa
     4981 ccggatgaat gaaacggaag ctgcacgaca tcagcgggaa attgcgcgta ttaatgccat
     5041 gcagtacgcc gaccaggctg tcagggatgc ggcgatacag cgtgaaaatg aacgttacga
     5101 aaaagccatt aagaaaaata cacgggcaac ccgtaatgat gaggccaccc ggttattgct
     5161 gcagtacagt cagcagcagg cacaggtgga agggcagatt gctgctgcca gacagtcagc
     5221 aggcattgcc actgaaagga tgactgaagc gcataaacag cttctggccc tgcagcagcg
     5281 catcagcgac ctggacggga aaaagctgac ggcagatgaa aagagtgtgc tggcccgtaa
     5341 gaatgagctg attcaggcgc tgacgctgct ggatgtgaaa cagcaggagc tgcagaaaca
     5401 gacagcgctt aacgacctga gaaaaaaaac ggttcagctg accagccagc tggcagacaa
     5461 agaacgtgca ctgcgtgagc agcacaatct ggatattgcc actgcaggta tgggggataa
     5521 gcagcggcag cgctaccagg cacagttgcg catccggcag gaataccggc aacagttgca
     5581 acagcttgag aatgacagtc gccagaaagg cacttacggg acggaggact accggagggc
     5641 tgaggaggtg ctgaagggga gcctgaagcg acaactgaat gaaaacaaac gctactggca
     5701 ggaactggaa gtggcgcagg gcgactggaa aaacggtgcc atgcgggcgt ttcagaattt
     5761 tacggcggat gcggataatg cggcgggaac ggcagaacag atgtttacag tggcattcag
     5821 cagtgccggt aatgcactgg cgacattctg taccaccggt aagctgaatt ttaaatcctt
     5881 cacctcttcc ctgttgtcag atatggccag aattatggca cagatggcca tgatgcaggc
     5941 ggtaaagggc gtcggttctt tattcggctt cacgactaat gctgatggcg gtgtttacca
     6001 gtctgctgat ttgagtcgct acagtggcac ggtggttaac cgtccgacgt tttttgcttt
     6061 tgcaaaaggc gcgggtgtga tgggggaagc gggacctgaa gccattctgc cactgcgtcg
     6121 cggtgctgat ggtaagctgg gggttgtggc ggatactggt ggttcaggca tggtgatgtt
     6181 tgccccgcag tacaacattg agatcaataa cgacggcacg aacgggcaga taggtccggc
     6241 tgccctgaag gtggtttatg acctcgggaa aaaagcggcc gcggacttta tgcaacagca
     6301 ggcccgtgat ggtggccggt taagtggagc ataccggtaa tggagacgtt tcactggaaa
     6361 gtgcgcccgg atatgaatgt ggtatcagag ccgaaagtgg tgacagtgaa gctgggcgat
     6421 ggttatgaac agcgtcgtgc ggcgggactg aataaccagt tgtcgactta cagcgtgacg
     6481 atacgtgttc gtaaaggtga acacccatct ttaaaagcct ttctggaacg gcacggtggc
     6541 gtccgcgcat ttcagtggac gccaccttat gactggaaac tgatcagggt ggtttgtcgt
     6601 aaatggtcgg caagcgtggg ggcgttatgg gtgactgtaa cggccgattt tgaacaggtg
     6661 gtgaactgat gcaggatatc cggcaggaaa cactgaatga atgcacccgt gcggagcagt
     6721 cagcccgggt ggagctctgg gaaatcgatc tgacagaggt cggtggtgag cgttattttt
     6781 tctgtaatga gcagaacgaa aaaggtgagc cggtcacctg gcaggggcgg cagtatcagg
     6841 cataccccat tcaggggtcg gggtttgaac tgaacggcag gggctgtgct gcccgtccga
     6901 cactgacggt ctctaacctg cacggcatgg tcaccgggat ggcggaagat ctgcagagtc
     6961 tggtcggcgg aacggtggtc cggcgtaagg tttacgcccg ttttctggat gcggtgaact
     7021 tcgtcaacgg aaacagcgac gccgatccgg aacaggaggt gatcagccgc tggcgcatcg
     7081 agcagtgcag cgaactgagc gcggtcagtg cctcttttgt gctggccaca ccaacggaga
     7141 cggatggcgc ggttttcccg gggcgtatca tgctggcgaa tacctgtatg tggacttacc
     7201 gttctgatga gtgtggttac acgggcaggg cagtggctga cgagttcgac aaaccaacaa
     7261 cggatatccg gaaggacaaa tgcagcaagt gtatgcgcgg gtgtgagttg cgcaacaata
     7321 ccggtaattt cggcggtttc ctttccatca ataaactttc tcagtaaatc catgacacag
     7381 acagaatcag cgattctggc gcacgcccgg cgatgtgcgc cagcggagtc gtgtggcttc
     7441 gtggtgagaa cgccggaggg agacaggtat cttcccagcg agaatatctc cggtgagccg
     7501 gaggaacggt tccggatggc tccggaggac tggctgcggg cacaaatgca gggtgagatt
     7561 gtggcactgg tccacagtca tcccggtggt ctgccctggc tgagtgaggc tgaccggcgg
     7621 ctgcaggtgc agagtgattt gccgtggtgg ctggtctgcc gggggacgat tcacaagttc
     7681 cgctgtgtgc cgcatctcac cgggcggcgc tttgagcacg gggtgacgga ctgttacacg
     7741 ctgttccggg atgcttatca tctggcgggg attgagatgc cggattttca tcgcggggat
     7801 gactggtggc gtcacggtca gaatctctat ctggataatc tggaggccac agggctgtat
     7861 caggtgccgt tgtcatcagc acaaccgggc gatgtgctgc tgtgctgttt tggttcatcg
     7921 gtgccgaatc atgccgccat ttactgcggc gatggtgagc tgctgcacca tattcctgaa
     7981 caactgagta aacgagagag gtataccgac aaatggcagc gacgcacaca ctccctctgg
     8041 cgtcaccggg aatggcacgc atctgccttt acggggattt gcaacgattt ggccgccgca
     8101 tcgaccttcg tgtgaaaacg ggggctgaag ccatccgcgc actggccaca cagctcccgg
     8161 cgtttcgtca gaaactgagt gacggctggt atcaggtgcg cattgccggg cgtgatgcag
     8221 gtgaaaccga attgtctgcc cgtcttaatg agccgctggc aaatggtgca gtgatccaca
     8281 tagtaccgcg tctggtggga gctaaaagtg gcggtgtgtt tcaggcggtg ctgggggcag
     8341 ctgttatggc ggttgctata tggatgccgg gggtaggaat tatggcgagt aatctgctgt
     8401 tttctctcgg tgccagtatg acgcttggcg gtgttgcaca gatgctggca ccgaaagcca
     8461 gaactccccg tacacagaca acggataacg gcaaacagaa cacctgtttc tcctcactgg
     8521 ataacatggt tgcccagggc aatgttctgc ctgttctgta cggtgaaatg cgcgtggggt
     8581 cgcgggtggt atctcaggag atcagcacgg cagacgaagg ggatggtggt caggttgtgg
     8641 tgattggtcg ctgatgcaaa atgtttcatg tgaaaccgcc tgcgggcggt tttgtcgttt
     8701 atggagcgtg aggaatgggt aaaggcagca gtaaggggca taccccgcgc gaagcgaagg
     8761 acaacctgaa atccacgcag ttgctgagtg tgattgatgc catcagcgaa gggccggttg
     8821 acggtccggt ggatggatta aaaagcgtgc tgctgaacgg tacgccggtc ctggatagcg
     8881 aggggaagac aaacttttcc ggtgttacgg tggtgttccg cgccggcgag caggagcaga
     8941 caccgccgga ggggtttgaa tcttccggct cagagactgt gctgggtacg gaagtgaaat
     9001 acgacacgcc gatcacccgg accatcacgt cggcaaacat tgatcgtctg cgcctgacct
     9061 tcggtgtgca ggcactggtg gaaaccacct caaaggggga ccggaatccg tcggaagtcc
     9121 gcctgctggt tcagatacag cgtaacggtg gctgggtgac ggaaaaagac atcaccatta
     9181 agggcaaaac cacttcgcag tatctggcct cggtggtggt gggtaacctg ccgccgcgcc
     9241 cgtttaatat ccggatgcgc aggatgacac cggacagcac cacagaccag ctgcagaaca
     9301 aaacgctctg gtcgtcgtac accgaaatca tcgatgtgaa acagtgctac ccgaacacgg
     9361 cgctggtcgg cgtgcaggtg gattcagagc agttcggtaa ccagcaggtg agtcgcaatt
     9421 atcatcttcg cgggcgcatt ctgcaggtgc cgtcgaacta taacccgcag acgcggcaat
     9481 acagcggtat ctgggacgga acgcttaagc cagcatacag caacaacatg gcctggtgtc
     9541 tgtgggacat gctcactcat ccgcgctacg gcatggggaa acgtcttggt gcggcagatg
     9601 tggacaaatg ggcgctgtat gtcatcggcc agtactgcga ccagtcagtg ccggacggat
     9661 ttggcggcac ggagccgcgc atcacctgta acgcttacct gaccacacag cgtaaggcgt
     9721 gggatgttct cagtgatttc tgctcggcga tgcgctgtat gccggtatgg aacgggcaga
     9781 cgctgacgtt cgtgcaggac cgaccgtcgg ataaagtgtg gacctataac cgcagtaatg
     9841 tggtgatgcc ggatgatggc gcgccgttcc gctacagctt cagcgccctg aaggaccgcc
     9901 ataatgtcgt tgaggtgaac tggattgatc cggacaacgg tcatgagacg gcgacagagc
     9961 ttgtggagga cacgcaggcc attgtccgtt acggtcgtaa cgtcacgaag atggatgcct
    10021 ttggctgtac cagccgggga caggcgcacc gcgccgggct gtggctgatt aaaacggaac
    10081 tgctggaaac gcagaccgtg gacttcagcg tcggcgcaga agggcttcgc catgtaccgg
    10141 gtgatgtcat tgaaatctgc gatgatgatt atgccggtat cagcaccggt ggtcgcgtac
    10201 tggcggtgaa cagccagacc cggacgctga cgctcgaccg tgaaatcacg ttgccgtcct
    10261 ccggtaccac gctgataagc ctggttgacg gaaatggcaa tccggtcagc gtggaggttc
    10321 agtccgtcac cgacggcgtg aaggtgaaag tgagccgggt tcctgacggc gttgcagaat
    10381 acagcgtgtg ggggctgaag ctgccgacgc tgcgccagcg cctgttccgc tgcgtgagta
    10441 tccgtgagaa cgacgacggc acgtatgcca tcaccgccgt gcagcatgtg ccggaaaaag
    10501 aggccatcgt ggataacggg gcacactttg acggtgaccg gcgcggcacg gtgaatggtg
    10561 tcacgccgcc agcggtgcag catctgaccg ccgaagtcac cgcagacagc ggggaatatc
    10621 aggtgctggc gcgatgggac acgccgaagg tggtgaaggg tgtgagcttc ctgcttcgcc
    10681 tgaccgtggc agcggacgac ggcagtgagc ggctggtcag cacagccagg acgacggaaa
    10741 ccacataccg cttcaggcag ctggcgctgg ggaattacag tctgacagtc cgggcggtaa
    10801 atgcccgggg gcagcagggc gatccggcgt cggtatcgtt ccggattgcg gcaccggcag
    10861 cgcctgtcac tattgaactg ataccggggt attttcagat aacggtggtc ccgaaactgg
    10921 ctgtatatga cccgacggtg cagtttgagt tctggttttc ggaaaagcgg attgcggata
    10981 tcaggcaggt tgaaaccagc gcccgctatc ttggcacggc gctgtactgg atagccgcca
    11041 gtatcaatat caggccgggc catgattatt atttttacgt tcgcagtgtg aacaccgttg
    11101 gcaaatcggc atttgtggag gccgtcggtc gggcgagcga tgatgcggaa ggttatctga
    11161 gtttttataa agggttgatc aataaaacgc atctcggcaa ggagttgtgg acgcagattg
    11221 ataacggtca gcttgcgccg gacctgactg aaatcaggac gtccattacg aatgtcagca
    11281 atgaaatcac gcaaaccgtc aataaaaaac tggaaaatca gagtgctgca atccagcaga
    11341 tacagaaagt tcaggttgat acaaataata acctgaacag catgtgggct gtgaagctgc
    11401 aacagatgaa ggacggacgc ctttatattg cgggtatcgg tgccggtatt gagaatacgc
    11461 cagcaggaat gcagagtcag gtgctgctgg cggcagacag gattgcgatg attaatcctg
    11521 cgaatggcaa cacaaagccg atgtttgttg gtcagggcga tcagatattc atgaacgacg
    11581 tgttcctgaa acgcctgacg gctccgacca ttaccagcgg cggtaatcct ccggcatttt
    11641 ccctgacacc tggcggacgg ctgacggcga aaaatgccga tatcagcggt aacgtgaacg
    11701 cgaactccgg gacgctcaac aacgtcacga ttaataagaa ctgtcgggtt ctgggaaaat
    11761 tgtccgcgaa ccagattgaa ggcgatctcg ttaaaacagt gggcaaacct ttcccacggg
    11821 actcccgggc accggagagg tggccatcag ggaccattac cgtcagggtt tatgacgatc
    11881 agccgtttga tcggcaaatt gttattcccg cggtggcgtt tcgcggtgct aaacatgagc
    11941 ggaagaataa caatatttat tcgtcatgcc gcctgatagt gaagaaaaac ggtgctgaaa
    12001 tttataaccg aacgaccctg gataatacgc tgatatatac gggtgttatt gatatgcctg
    12061 ccggtcacgg tcacatgacg ctggagtttt ctgtatcggc atggctggta aatggctggt
    12121 atcccacagc aagtatcagc gatttgctgg ttgttgtgat gaagaaatcc acagcaggta
    12181 tcagtatcag ctgaatttta taacccagat acgggcacca gaaatggtgc cttttttatt
    12241 gcagaaaagc gagaggtaat tatgcgtaaa ctttatgctg ccattttgtc cgcagccatc
    12301 tgtctggctg tatccggtgc gc
//
LOCUS       DAZXUL010000116        12267 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZXUL010000116 DAZXUL010000000
VERSION     DAZXUL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12267
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.116"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            758..841
                     /gene="dinQ"
                     /locus_tag="ACH3L1_003334"
     CDS             758..841
                     /gene="dinQ"
                     /locus_tag="ACH3L1_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFT4077959.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACH3L1_003335"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACH3L1_003335"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFT4077960.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACH3L1_003336"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACH3L1_003336"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFT4077961.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="ACH3L1_003337"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="ACH3L1_003337"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFT4077962.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACH3L1_003338"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACH3L1_003338"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFT4077963.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="ACH3L1_003339"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="ACH3L1_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            7850..7903
                     /locus_tag="ACH3L1_003340"
     CDS             7850..7903
                     /locus_tag="ACH3L1_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077964.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="ACH3L1_003341"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="ACH3L1_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFT4077965.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="ACH3L1_003342"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="ACH3L1_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFT4077966.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="ACH3L1_003343"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="ACH3L1_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFT4077967.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="ACH3L1_003344"
     CDS             10967..12166
                     /locus_tag="ACH3L1_003344"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFT4077968.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         2997 a         3120 c         3295 g         2855 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggtgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagc
//
LOCUS       DAZXUL010000117        12219 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZXUL010000117 DAZXUL010000000
VERSION     DAZXUL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.117"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..493)
                     /locus_tag="ACH3L1_003345"
     CDS             complement(<1..493)
                     /locus_tag="ACH3L1_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFT4077969.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="ACH3L1_003346"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="ACH3L1_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFT4077970.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="ACH3L1_003347"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="ACH3L1_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFT4077971.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="ACH3L1_003348"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="ACH3L1_003348"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFT4077972.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="ACH3L1_003349"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="ACH3L1_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFT4077973.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="ACH3L1_003350"
     CDS             3646..4155
                     /locus_tag="ACH3L1_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFT4077974.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="ACH3L1_003351"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="ACH3L1_003351"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFT4077975.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="ACH3L1_003352"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="ACH3L1_003352"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFT4077976.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="ACH3L1_003353"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="ACH3L1_003353"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFT4077977.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="ACH3L1_003354"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="ACH3L1_003354"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFT4077978.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="ACH3L1_003355"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="ACH3L1_003355"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFT4077979.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="ACH3L1_003356"
     CDS             complement(8797..9690)
                     /locus_tag="ACH3L1_003356"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFT4077980.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="ACH3L1_003357"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="ACH3L1_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="ACH3L1_003358"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="ACH3L1_003358"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFT4077981.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11580..>12219
                     /gene="allE"
                     /locus_tag="ACH3L1_003359"
     CDS             11580..>12219
                     /gene="allE"
                     /locus_tag="ACH3L1_003359"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFT4077982.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2965 a         3158 c         3118 g         2978 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatacaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 ccacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgctg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg caggaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttata
//
LOCUS       DAZXUL010000118        12152 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZXUL010000118 DAZXUL010000000
VERSION     DAZXUL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12152
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.118"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            126..995
                     /locus_tag="ACH3L1_003360"
     CDS             126..995
                     /locus_tag="ACH3L1_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFT4077983.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="ACH3L1_003361"
     CDS             complement(1198..1551)
                     /locus_tag="ACH3L1_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFT4077984.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACH3L1_003362"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACH3L1_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFT4077985.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="ACH3L1_003363"
     CDS             complement(3379..3672)
                     /locus_tag="ACH3L1_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFT4077986.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="ACH3L1_003364"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="ACH3L1_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFT4077987.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /locus_tag="ACH3L1_003365"
     CDS             complement(5220..5696)
                     /locus_tag="ACH3L1_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFT4077988.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="ACH3L1_003366"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="ACH3L1_003366"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFT4077989.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="ACH3L1_003367"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="ACH3L1_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFT4077990.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="ACH3L1_003368"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="ACH3L1_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFT4077991.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="ACH3L1_003369"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="ACH3L1_003369"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFT4077992.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="ACH3L1_003370"
     CDS             11052..11627
                     /locus_tag="ACH3L1_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFT4077993.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="ACH3L1_003371"
     tRNA            11734..11809
                     /locus_tag="ACH3L1_003371"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2931 a         3140 c         2980 g         3101 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa ca
//
LOCUS       DAZXUL010000119        12119 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZXUL010000119 DAZXUL010000000
VERSION     DAZXUL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12119
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.119"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            169..1310
                     /gene="yghO"
                     /locus_tag="ACH3L1_003372"
                     /pseudo
     CDS             169..1310
                     /gene="yghO"
                     /locus_tag="ACH3L1_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            complement(1434..2204)
                     /gene="glcC"
                     /locus_tag="ACH3L1_003373"
     CDS             complement(1434..2204)
                     /gene="glcC"
                     /locus_tag="ACH3L1_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFT4077994.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2455..3954
                     /gene="glcD"
                     /locus_tag="ACH3L1_003374"
                     /pseudo
     CDS             2455..3954
                     /gene="glcD"
                     /locus_tag="ACH3L1_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            3954..5006
                     /gene="glcE"
                     /locus_tag="ACH3L1_003375"
     CDS             3954..5006
                     /gene="glcE"
                     /locus_tag="ACH3L1_003375"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFT4077995.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            5017..6236
                     /gene="glcF"
                     /locus_tag="ACH3L1_003376"
                     /pseudo
     CDS             5017..6236
                     /gene="glcF"
                     /locus_tag="ACH3L1_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6241..6645
                     /locus_tag="ACH3L1_003377"
     CDS             6241..6645
                     /locus_tag="ACH3L1_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFT4077996.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            6667..8838
                     /gene="glcB"
                     /locus_tag="ACH3L1_003378"
     CDS             6667..8838
                     /gene="glcB"
                     /locus_tag="ACH3L1_003378"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFT4077997.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9193..10779
                     /locus_tag="ACH3L1_003379"
     CDS             9193..10779
                     /locus_tag="ACH3L1_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HFT4077998.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         2948 a         2897 c         3275 g         2999 t
ORIGIN      
        1 aaattgacaa aaggcattga ggcgtttctg tgtagcctct gaccttatca taatcattaa
       61 ttaatactat ttattcgtcg tttgtattca ttgagttgat tattaagatg ccgctcgttg
      121 ctgttgcaac ctgtttttca ccattaatta tttgttatcc acagagatat ggaatgcgat
      181 ctgctgatga ttaaaattga aaaagtaatt aataaaaatg accttaaggc atttatcgct
      241 tttccgtcat cactttatcc cgacgatcca aactggatac cccctttatt cattgaacgc
      301 aacgagcatt tgtctgcgaa aaatccgggg acggaccata tcatctggca ggcgtgggtg
      361 gcaaaaaaag cggggcagat agttgggcgt attaccgcgc aaatagatac cttgcaccgt
      421 gagcgttacg gtgaagatac cggtcatttc ggcatgattg acgccattga tgatccgcag
      481 gattttgccg cgttgtttgg cgcagcggaa gcgtggttga agtcacaagg tgcaagtaag
      541 atcagcgggc ctttcagcct gaatatcaat caggaaagcg gattactgat tgaaggtttt
      601 gacacaccac cctgtgcgat gatgccacac ggcaaaccgt ggtatgccgc gcatattgaa
      661 caactgggtt atcacaaagg cattgattta ctggcgtggt ggatgcagcg aaccgatctc
      721 actttctctc cggcgctaaa aaaactgatg gatcaggtgc gcaaaaaggt gaccattcgc
      781 tgcatcaatc gtcagcggtt tgccgaagag atgcagatcc tgcgtgagat tttcaactct
      841 ggctggcagc acaactgggg atttgtgccg tttaccgaac atgaattcgc gaccatgggc
      901 gaccaactta aatatctggt gccggacgat atgatctaca tcgctgagat tgattctgca
      961 ccctgcgcgt ttattgtggc ttgcctaaca tcaacgaggc gattgccgat ctgaacggat
     1021 cgctctttcc cttcggctgg gcaaaattgc tgtggcgttt gaaagtcagc ggtgtgcgaa
     1081 ctgcgcgagt accgctgatg ggcgtgcgcg acgagtatca gttcagccgc atcggcccgg
     1141 tgattgcgct gttattgatt gaagccttac gcgatccgtt tgcccgccgg aagatcgatg
     1201 cgctggagat gtcatggatc ctcgaaacca atacaggcat gaataacatg ctggaacgca
     1261 ttggggcgga gccgtacaag cgttaccgat tgtatgaaaa gcaaatttaa taccagatcc
     1321 tgtttagcgc aggggcggtc attcgggaga gggtatagat gaacagattt aacaccagat
     1381 cctgttaagt gcaggggcgg tcccctcgcc ttggggatag ggtaaaactc tgctcacatt
     1441 cccaccttgt tcatctccag cggcaccgcc gagcgaatca aatggtggcc ttccagctcg
     1501 atatcgtgga gattcttttt cacggtccgt acatgatcgc gcgctgcgcg ctgagcgacg
     1561 tgcggcagcc gctgcaacac cgcgttgtag atccgcgcat gctggcgatc gatctgcttt
     1621 ttttgctgtg gtcgatggta gagattattt acgctggcaa acactgaatt aaacatcaga
     1681 tcggtcaatg attgcagcgt aaacaccagc acctgattgt gagaagcctg acaaatggcg
     1741 agatggaaag cgtgatccaa ctgcgcatgt tcgatcagcg aaatctcttt gtggttctca
     1801 ctggcggcga gcattttttc ataacagcgg gttatcacaa caaaatcagc ctgcgttccc
     1861 agcgttgccg ccagccttgc cgattcgccc tccagtaatg cgcgaacgtc gagcagatcg
     1921 tacagcgttc gcggctgcgt actgaacaga tggatcagcg ggctggtgtc ctgctcccga
     1981 ttaagccgtg cgacatgaga atcgcgaccc tgcgccgttt caataatccc gcgcccgcgc
     2041 agcacggtca gcccttcacg cagtgcggag cgtgagaagc cgagcttttc acacagtcga
     2101 cgttccgagg gaagcggctg accgaccttc agtacgccgt cgataattaa ccgttcgata
     2161 ctctctgcaa ccacttcgca aatagggcga cgttcatctt tcattcccgg acctcgtgca
     2221 caggtaggac caatttttct gttcttaact cgcaaaacac gcacatcacg caagtgatta
     2281 tgttacatca atttaacatt aagttaacca agacaaggtc acagagctgg aaaaaaaatg
     2341 gtctgaccgg tagctaaaga gatagacgaa aacgaaaagc ccgcttaata actgttcaca
     2401 gaagcagcgc gcaaaaatca gctgccacac aacacaacaa agcgaagcct actcatgagc
     2461 atcttgtacg aagagcgtct tgatggcgct ttacccgatg tcgaccgcac atcggtactg
     2521 atggcactgc gtgagcatgt ccctggactt gagatcctgc ataccgatga ggagatcatt
     2581 ccttacgagt gtgacgggtt gagcgcgtat cgcacgcgtc cattactggt tgttctgcct
     2641 aagcaaatgg aacaggtgac agcgattctg gctgtctgcc atcgcctgcg tgtaccggtg
     2701 gtgacccgtg gtgcaggcac cgggctttct ggtggcgcgc tgccgctgga aaaaggtgtg
     2761 ttgttggtga tggcgcgctt taaagagagc ctcgacatta accccgttgg tcgccgcgcg
     2821 cgcgtgcagc cgggcgtatg taacctggcg atctcctagg ccgttgcgcc gcataatctc
     2881 tattacgcgc cggatccttc ctcacaaatc gcctgttcaa ttggcggcaa tgtggcggaa
     2941 aatgccggtg gcgtccactg cctgaaatat ggtctgaccg tacataacct gctgaaaatt
     3001 gaagtgcaaa cgctggacgg cgaggcgctg acgctgggat cggacgcgct ggattcacct
     3061 ggttttgacc tgctggcgct gttcaccgga tcggaaggta tgctcggcgt gaccaccgaa
     3121 gtgacggtaa aactgctgcc gaagccgccc gtggcgcggg ttctgttagc cagctttgac
     3181 tcggtagaaa aagccggact tgcggttggt gacatcatcg ccaatggcat tatccccggc
     3241 gggctggaga tgatggataa cctgtcgatc cgcgcggcgg aagattttat tcatgccggt
     3301 tatcccgtcg acgccgaagc gattttgtta tgcgaactgg acggcgtgga gtctgacgta
     3361 caggaagact gcgagcgggt taactacatc ttgttgaaag cgggcgcgac tgacgtccgt
     3421 ttggcacagg acgaagcaga gcgcgtacgt ttctgggccg gtcgcaaaaa tgcgttcccg
     3481 gcggtaggac gtatctcccc ggattactac tgcatggatg gcaccatccc gcgtcgcgcc
     3541 ctgcctggcg tactggaagg cattgcccgt ttatcgcagc aatatgattt acgtgttgcc
     3601 aacgtctttc atgccggaga tggcaacatg cacccgttaa tccttttcga tgccaacgaa
     3661 cctggtgaat ttgcccgcgc ggaagagctg ggcgggaaga tcctcgaact ctgcgttgaa
     3721 gttggcggca gcatcagtgg ggaacatggc atcgggcgag aaaaaatcaa tcaaatgtgc
     3781 gcccagttca acagcgatga aatcacgacc ttccatgcgg tcaaggcggc gtttgacccc
     3841 gatggtttgc tgaaccctgg gaaaaacatt cccacgctac accgctgtgc tgaatttggt
     3901 gccatgcatg tgcatcacgg tcatttacct tttcctgaac tggagcgctt ttgatgctac
     3961 gcgagtgtga ttacagtcag gcgctgctgg agcaggtgaa tcaggcaatt agcgataaaa
     4021 cgccgctggt gattcagggc agcaatagca aagccttttt aggtcgccca gtcaccgggc
     4081 aaacgctgga tgtgcgttgt catcgcggca ttgttaatta cgacccgacc gagctggtga
     4141 taaccgctcg tgccggaacg ccgctggtgg cgattgaagc ggcgctggag agcgccgggc
     4201 aaatgcttcc ctgcgagccg ccgcattatg gtgaagaagc cacctggggc gggatggtcg
     4261 cctgcgggct ggcggggccg cgtcgcccgt ggagcggttc ggtccgcgat tttgtcctcg
     4321 gcacgcgcat cattaccggc actggaaaac atctgcgttt tggtggcgaa gtgatgaaaa
     4381 acgttgccgg atacgatctc tcacggttaa tggccggaag ctacggttgt cttggcgtgc
     4441 tcactgaaat ctcaatgaaa gtgttaccgc gaccgcgtgc ctctctgagc ctgcgtcggg
     4501 aaatcagcct gcaagaagcc atgagtgaaa tcgccgagtg gcaactccag ccattaccca
     4561 ttagtggctt atgttacttc gacaatgcgt tgtggatccg ccttgagggc ggcgaaggat
     4621 cggtaaaagc agcgcgtgaa ctgctgggtg gcgaagaggt tgtgggtcag ttctggcagc
     4681 aattgcgtga acaacaactg ccgttcttct cgttaccagg taccttatgg cgcatttcat
     4741 tacccagcga tgcgccgatg atggatttac ccggcgagca actgatcgac tggggcgggg
     4801 cgttacgctg gctgaaatcg acagccgatg acaatcaaat ccatcgcatc gcccgcaacg
     4861 ctggcggtca tgcgacccgc tttagtgccg gagatggtgg ctttgccccg ctatcggctc
     4921 ctttattccg ctatcaccag cagcttaaac agcagctcga cccttgcggc gtgtttaacc
     4981 ccggtcgcat gtacgcggaa ctttgaggag caggctatgc aaacccgatt aaccgaagag
     5041 atgcggcaga acgcgcgctg gaagccgaca gcatcctgtg cgcctgtgtt cactgcggat
     5101 tttgtaccgc aacctgccca acctatcagc ttctgggcga tgaactggac gggccgcgcg
     5161 ggcgcatcta tctgattaaa caggtgctgg aaggcaacga agtcacgctt aaaacacagg
     5221 agcatctcga tcgctgcctc acttgccgca attgtgaaac cacctgtcct tctggtgtgc
     5281 gctatcacaa tttgctggat atcgggcgtg atattgtcga gcagaaagtg aaacgcccac
     5341 tgccggagcg aatgctgcgc gaaggattgc gccaggtagt gccgcgtccg gcggtcttcc
     5401 gtgcgctgac gcaggtaggg ctggtgctgc gaccgttttt accggaacag gtcagagcaa
     5461 aactgcctgc tgaaacggtg aaagctaaac cgcgtccgcc gctgcgccat aagcgtcggg
     5521 ttttaatgct ggaaggctgc gcccagccta cgctttcgcc caacaccaac gcggcaactg
     5581 cgcgagtgct ggatcgtctg gggatcagcg tcatgtcagc taacgaagca ggctgttgtg
     5641 gcgcggtgga ctatcatctt aatgcgcagg agaaagggtt ggcacgggcg cgcaataata
     5701 ttgatgcctg gtggcccgcg attgaagcag gtgccgaggc aattttgcaa accgccagcg
     5761 gttgcggcgc gttcgtcaaa gagtatgggc agatgctgaa aaacgatgcg ttatatgccg
     5821 ataaagcgcg tcaggtcagt gaactggcgg tcgatttagt cgaacttctg cgcgaggaac
     5881 cgctggaaaa actggcaatt cgcggcgata aaaaactggc cttccactgc ccgtgtaccc
     5941 tacaacatgc gcaaaagctg aacggcgaag tggaaaaagt gttacttcgt cttggattta
     6001 ccttaacgga cgttcccgac agccatctgt gctgcggttc agcgggaaca tatgcgttaa
     6061 cgcatcccga tctggcacgc cagctgcggg ataacaaaat gaatgcgctg gaaagcggca
     6121 aaccggaaat gatcgtcacc gccaacattg gttgccagac gcatctggcg agcgccggtc
     6181 gtacctctgt gcgtcactgg attgaaattg tagaacaagc ccttgaaaag gaataaaaaa
     6241 atgaaaacta aagtcattct tagccagcaa atggcgagtg caattattgc cgcaggtcag
     6301 gaagaggcgc agaaaaataa ctggtctgtt tccattgctg ttgccgatga cggcggtcat
     6361 ctgctggcgt taagtcgcat ggacgattgc gcgccgattg cggcttacat ctcccaggag
     6421 aaagcgcgta ccgccgcgct ggggcgtcgt gaaactaagg gctatgaaga gatggtgaac
     6481 aacggacgta ccgcgttcgt gactgcgccg ttactgacgt cgctggaagg cggcgtaccg
     6541 gttgttgttg atgggcaaat tattggtgcc gtgggcgttt ctggtttaac cggagcacag
     6601 gatgcgcagg tcgcgaaagt ggcagcagcg gtgttggcga aataagcgaa aacgaggaga
     6661 gaaacaatga gtcaaaccat aacccagggc cgtttacgca ttgacgccaa ttttaaacgt
     6721 tttgtggatg gagaagtttt gccgggcgtg gaactggacg ctgctgcatt ctggcacaat
     6781 gttgatgaga tcgttcacga tctggcacca gaaaatcgtc agttgctggc agaacgcgat
     6841 cgcattcagg cggcgcttga tgagtggcat cgcagcaatc cggggccggt aaaagataaa
     6901 gcggcctata aatctttcct tcgtgaactg ggctacctgg tgccgcaacc ggagcgcgtg
     6961 acggtggaaa ccacgggcat tgacagcgaa atcaccagtc aggcggggcc acagctggtg
     7021 gttccggcaa tgaacgcccg ctacgcgctg aacgcggcga acgctcgctg gggctcactg
     7081 tacgatgcgt tatacggcag cgacatcatc ccgcaggaag gggcgatggt cagtggctac
     7141 gatccgcagc gcggtgagca ggttatcgcc tgggttcgac gtttcctcga tgaatcacta
     7201 ccgctggaaa acggcagcta tcaggatgtg gtggcgttta aggtggtcga taaacaatta
     7261 cgcatccagt tgaaaaatgg taaagaaacc acgttacgta ctccagcaca gtttgtcggt
     7321 taccgtggcg atgccgctgc gccgacctgc attttgctga aaaataacgg cctgcatatt
     7381 gaactgcaaa ttgatgccaa cgggcggatt ggcaaagacg atccggcgca catcaacgat
     7441 gttatcgtcg aagctgctat cagtaccatt ctcgactgcg aagattcggt cgcggcggtt
     7501 gatgcggaag ataaaatcct gctgtaccgc aatctgctgg gcctgatgca ggggactctg
     7561 caagagaaaa tggagaaaaa cggtcggcaa atcgtacgta aactgaatga cgatcgtcag
     7621 tacaccgccg ccgatggctc tgaaatttct ctgcacggac gctcgttgct gtttatccgc
     7681 aacgtgggtc atttgatgac cattcctgtg atttgggaca gcgaaggcaa tgaaatcccg
     7741 gaaggtattc ttgatggcgt catgactggc gcgattgccc tctatgattt aaaagtgcag
     7801 aaaaactcgc gcactggcag cgtctatatt gtgaaaccga aaatgcacgg tccgcaggaa
     7861 gtggcgttcg ccaacaaact gtttacccgc attgagacaa tgctcggtat ggcaccgaat
     7921 accctgaaaa tgggcattat ggatgaagaa cgccggacct cgctgaactt gcgtagctgt
     7981 atcgctcagg cgcgcaaccg cgtggcgttc atcaataccg gtttcctcga ccgtaccggc
     8041 gatgaaatgc attcggtgat ggaagctggc ccgatgctgc gtaaaaacca gatgaaatcg
     8101 acgccgtgga tcaaagccta cgaacgtaat aacgtgcttt ccggtctgtt ctgtgggctg
     8161 cgcggtaaag cgcaaatcgg taaaggcatg tgggcaatgc cggacctgat ggcagacatg
     8221 tacagccaga agggcgacca actgcgtgcc ggggcaaaca cagcctgggt tccgtcacca
     8281 accgctgcta cgctccatgc gctgcactac caccatacca acgtacagag cgtgcaagcc
     8341 aacattgccc agaccgagtt cagtgctgaa tttgaaccgc tgctggacga tctgctgact
     8401 attccggtgg ctgaaaacgc taactggtcg gcgcaagaga tccaacaaga gctggataac
     8461 aacgtgcagg ggattctggg gtacgtggtg cgttgggtgg agcaggggat tggttgttca
     8521 aaagtgccgg atattcacaa cgtggcgctg atggaagacc gcgcaacgct gcgtatctcc
     8581 agccagcata tcgccaactg gttacgtcac ggtattctga ccaaagaaca ggtgcaggcg
     8641 tcgctggaga atatggcgaa agtggttgat cagcaaaatg ctggcgatcc ggcttatcgt
     8701 ccgatggcgg ggaatttcgc taactcgtgt gcttttaaag ctgccagcga tttaatcttc
     8761 ctcggcgtga aacagccaaa cggttatacc gaaccgttat tacacgcctg gcgtttacgc
     8821 gaaaaagaaa gtcattaata gcgtcaaaaa agccccggca agttttattg tcggggctat
     8881 gatagtaact gcggtagtac atttatcccg gaaataaccg gagcgattat cagggtttaa
     8941 caatattaaa tacccgtagc gcattactag cgggcctaaa acaacaatat ggacatctta
     9001 acgatactct gcgcctgcgt gtgcggaaca gaaaccctta cccgatatac ttgttatgcc
     9061 agaggtagca attgcgttag cggcgtactg ttgctgtaaa gtataacgcc tatattttac
     9121 agttcagtcc tcttttgctt attacaacct taagtatatc aagcatataa agataataag
     9181 agactgaaca atatggttac ctggacccaa atgtatatgc cgatgggagg actggggcta
     9241 tccgctctgg tcgccctgat cccgataata ttcttcttcg ttgcactcgc ggtattacgt
     9301 ctgaaaggac atgttgctgg agcaataacc cttatattat ctatcctgat tgcgatattc
     9361 gcctttaaaa tgccgattga tatggcattt gctgctgcgg gctatggctt tatttatgga
     9421 ttatggccaa ttgcgtggat tattgtcgcg gcggtgttcc tgtataaatt aaccgttgcc
     9481 agcgggcagt tcgatattat ccgcagctcg gttatctcca tcaccgacga tcagcgtttg
     9541 caggtgttac tgattggttt ctcctttggt gcgttgctgg aaggagctgc tggctttggt
     9601 gcgacggtgg cgtttggtgc gttgggcgtg ccgattctgg tcgccggtca ggtaacggga
     9661 atcgatccgt tccacattgg cgcaatggcg ggacgtcagt taccgttcct gtcggttctt
     9721 gtgccgttct ggctggtagc aatgatggac ggctggaaag gggtgaaaga gacgtggcca
     9781 gcggcgctgg ttgctggggg aagcttcgct gtcactcagt tctttacctc taactatatt
     9841 ggtccggaac tgccggatat tacttcggcg ctggtgagta tcgtctcact cgctttattc
     9901 cttaaagtct ggcggccgaa aaataccgaa acggcaatca gcatggggca atccgcaggt
     9961 gcgatggtgg taaataagcc atcttctggc ggtcccgtgc cttcagaata tagtctgggg
    10021 caaatcattc gtgcgtggtc accgttttta atcttaacgg tgctggtcac catctggacc
    10081 atgaagccgt ttaaagcgtt atttgctccg ggcggcgcgt tttattcact ggtgattaat
    10141 ttccagatcc ctcatttgca tcaacaagtg ttgaaagcgg cacccattgt cgcccaacca
    10201 acgccaatgg atgcggtgtt taaattcgac cctctctcgg ctggcggcac cgctattttt
    10261 attgcggcga ttatctctat cttcatcctc ggcgtgggca tcaagaaagg gattggcgtc
    10321 tttgccgaaa tgctaattag tctgaagtgg ccgatattgt cgattggcat ggtgctggca
    10381 ttcgccttcg tcaccaacta ttccggcatg tccaccacgc tggcgctggt actggcaggt
    10441 acaggcgtga tgttcccgtt tttctcaccg tttctcggct ggctgggcgt attccttacc
    10501 gggtcggaca cttcttcaaa cgccctgttt ggttcactgc aatcgaccac ggcgcagcaa
    10561 atcaacgtct ctgacactct gctggtggca gcaaatacca gcggcggcgt aactggcaag
    10621 atgatctccc cgcaatctat cgccgtggcc tgcgccgcga cgggcatggt gggccgagaa
    10681 tctgaactgt tccgctacac cgtgaagcac agtctgattt ttgccagcgt tatcaccctg
    10741 ctgcaggcgt atgtgtttac cgggatgtta gtctcgtaaa tcaataaagc cggatgaagg
    10801 taaatatcac ccggctttcc atttgtctgt cgtttatctc gccagggaac cgtttttacg
    10861 attggcttct ttatacgtag tctccgtttt acgatgcgaa ttgtttaata tttatacaac
    10921 ttggcttctg tgctggttat aaagtaccca ctttcatgag atcaatttta gtatgttatt
    10981 acattgcttc atctgttgct ttcatgacga atggcgtttt ttctcttatt tatatttttc
    11041 actagaacaa tgagtagatc aaaattccca aaactcccaa aaacataaga ttattcgcaa
    11101 agctgatttt gcagaataat gagaactttt aagatttgtc gaggatcaat tttaagcaat
    11161 ttatctccac ttgttacata aaagatgccg ttaggttaat aagttaatct tgctataaca
    11221 gtgttcgtta ctgcctatta ataaagatat ttgggatagg gtaacgtgga tgaggattcg
    11281 aaatcatccg tcttgcggaa agtgaaatgt tggcagatgt taatattatg tatgtatttt
    11341 aactccaaca acacaccaat cattttgtta gaaataagaa aatttatggt taaataattt
    11401 ggtgattatg aagcattaaa tcgtaatgcc aaatattgtt cgctttggag agtattattg
    11461 cctgtttgtg tctgttgagc agaaaaatca tctgaatttg aatactttta ttaggtgtga
    11521 ataaatgaaa aaatcattaa aatcattaaa atcattaaaa tcattaaaat cattaaaatc
    11581 attaacttat atgaatattt cagagttgga gttcgaatgt tgacacctga gtatttcctg
    11641 ctaaaaatag ccctgcttta cggatggttg tattgcaata acacgaaagt agtaagagat
    11701 taaaggtatc tgtcagcgat aactctgcaa gtgatgacat tttgtaaacc aaataatgta
    11761 aatcagcaat gtatcttttc acttaattat aacaaatgtg gtattcacta agaatctgat
    11821 acgagcttac atcacgacta ttttagtctc atcgcgggat acagggatgc accgatggat
    11881 ctcagcacac cgtaatgcaa tacctgaaca aatagaacag taaagtggtt cagtattaat
    11941 gaaagctctt attttccgtt gggaaaactt aaaatagatg agaaggcttt ttgatttctt
    12001 atgtgatctc atttcgagta ttgagaaatt tgtgattttt aaatttttca ataagccctg
    12061 taagggattg gggttttatg agaaataaag agacgtattg tctttgttta aacggtacg
//
LOCUS       DAZXUL010000120        12113 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZXUL010000120 DAZXUL010000000
VERSION     DAZXUL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.120"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..371
                     /locus_tag="ACH3L1_003380"
     CDS             <1..371
                     /locus_tag="ACH3L1_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4077999.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="ACH3L1_003381"
     CDS             complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="ACH3L1_003381"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFT4078000.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="ACH3L1_003382"
     CDS             complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="ACH3L1_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFT4078001.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="ACH3L1_003383"
     CDS             complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="ACH3L1_003383"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFT4078002.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="ACH3L1_003384"
     CDS             complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="ACH3L1_003384"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFT4078003.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3449..5050)
                     /gene="tap"
                     /locus_tag="ACH3L1_003385"
     CDS             complement(3449..5050)
                     /gene="tap"
                     /locus_tag="ACH3L1_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFT4078004.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5096..6757)
                     /gene="tar"
                     /locus_tag="ACH3L1_003386"
     CDS             complement(5096..6757)
                     /gene="tar"
                     /locus_tag="ACH3L1_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFT4078005.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="ACH3L1_003387"
     CDS             complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="ACH3L1_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFT4078006.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="ACH3L1_003388"
     CDS             complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="ACH3L1_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFT4078007.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9395..10321)
                     /gene="motB"
                     /locus_tag="ACH3L1_003389"
     CDS             complement(9395..10321)
                     /gene="motB"
                     /locus_tag="ACH3L1_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFT4078008.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10318..11205)
                     /gene="motA"
                     /locus_tag="ACH3L1_003390"
     CDS             complement(10318..11205)
                     /gene="motA"
                     /locus_tag="ACH3L1_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFT4078009.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="ACH3L1_003391"
     CDS             complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="ACH3L1_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFT4078010.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11913..>12113)
                     /locus_tag="ACH3L1_003392"
     CDS             complement(11913..>12113)
                     /locus_tag="ACH3L1_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFT4078011.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2783 a         3388 c         2934 g         3008 t
ORIGIN      
        1 acactacgct aatgagttgg gatgccgtgc tctatgatct acgtagtgaa aaaattatca
       61 gtaaagataa ttacttgacc gacttaaacc gcagacatct tgaacttgtt cttcatacta
      121 accccaatcc aagcggttcg gtaacgaaga ttttacgcac tatttttact aagcaggtaa
      181 agagtttagg gccggaactc tgtcatttct tgcaccatga actgacgaaa aaacgttatg
      241 aagtatggca tcactacgat gtactgcact accagtcatc gtcattttct cgttcacaat
      301 ttaatgcatt gataaattta ctggcgcaac aatcacgctt cgctgatcta aaattatcaa
      361 actacaaata accatcctcc acgtactccc tgaacgatac ccgccaccct gctatcggct
      421 tggcatcatc gcgcaggccg aagttcaggc aatacaaatc aaaatccaag actatccaac
      481 aaatcgtcca cctgatcctg actggctacc acgccggctt tgctggtatc gacctgaggt
      541 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
      601 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata
      661 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
      721 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
      781 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
      841 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
      901 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
      961 atggcttccg ccgcttcggc aatggcctga accagcccca gttcccgcaa actgtcgcgc
     1021 agcatatgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca
     1081 ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt
     1141 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     1201 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     1261 caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt
     1321 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     1381 gtcgacacca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     1441 cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     1501 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaattgt tgccaacatt
     1561 tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg
     1621 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     1681 tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca
     1741 accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga
     1801 tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat aatttgcgcc actacgcgcc
     1861 agttccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac acgttctccg
     1921 tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg
     1981 gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag cggcaacggt
     2041 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     2101 tcagaactca acaacggccc tgccttcagc gttgtcggtg ctgacaatgg cttatgtgct
     2161 gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     2221 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc
     2281 gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag aaaccatcac aacgggcatt
     2341 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca
     2401 tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcaac cagcggatca
     2461 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
     2521 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
     2581 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcgaggtgg ctaaagtttt
     2641 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
     2701 tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
     2761 acggccccgg cacggtgtac tgtttcgcca gtaaattcag cggggcaaaa tcaacatagt
     2821 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
     2881 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgatag ataccgcttc
     2941 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggca
     3001 cggtgcccaa tgtgtcagcc agagtcatcg caatgctgta cggctcttcg ccggtcgaag
     3061 ccgccgcgct ccatacgcga tcctcgccag aacggcgacg tgcgtgatcc gcgagcagag
     3121 ggaaatgatg agcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
     3181 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
     3241 tcagtcccag agaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt
     3301 cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
     3361 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
     3421 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
     3481 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
     3541 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg
     3601 agttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
     3661 gccgttgttc ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg
     3721 aactgacaat atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
     3781 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
     3841 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
     3901 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
     3961 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
     4021 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
     4081 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
     4141 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat
     4201 tgcctgcgac aatctccgcg atcccaatgt gcatctcatg gcttccctta cgcacatcac
     4261 tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct
     4321 cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat
     4381 ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc
     4441 tgatgtagat cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt
     4501 tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct
     4561 caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa
     4621 gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac
     4681 ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc atcgccataa
     4741 aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt
     4801 taatgtcatc cgccggatag ctaagcgctg tcagagtgcc tgctttgttc agcgcggtac
     4861 tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct
     4921 cgccgtgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc
     4981 caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac
     5041 gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaaa actcgtcaaa
     5101 atgtttccca gtttggatct tgttcagtaa ttcgcagtcg tggctgtgcc ggtggttgct
     5161 cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga
     5221 acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagct gcggcggcag
     5281 ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca
     5341 atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa
     5401 tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga
     5461 ccagcaccga accggtatca atgcgcgaga cggagtcttc aatgagggct ttgatctctt
     5521 ttgccgccag ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac
     5581 cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag
     5641 tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg
     5701 cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt
     5761 cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg
     5821 tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag
     5881 tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat
     5941 ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc
     6001 tctgcgccag gtcacccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac
     6061 caccggcgat ttcgcaaatg tgagcaataa tttttgccag cggagtaagc aacatacggc
     6121 gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt
     6181 gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt
     6241 cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg
     6301 gctgagcgaa ataagctcca gtattgccat aatcaagata atcaatcagt tcagttaacg
     6361 ctgtgtaata gtttttatat ttttcatcaa aattacggct ggtagcgacc atttcaggta
     6421 acggtgccat gcttttgaat tttttataat gcgtcgcggc ctgggccaat gttttcctgg
     6481 cgctatcgag caattcaact ttggcgttac tttgttgatt ggaggaatcc atcatcatcc
     6541 gcaccgctga acgactcagg ttaatgcgcg tttgcagcat taaatcccag gttgacgtca
     6601 gctcgccctg ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa
     6661 gggaagaaaa aaacaaactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca
     6721 ttaccaacag cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt
     6781 tgatctcgtt atcggcaaga aggggggaaa ctttattgct gatgccaccc gccgcgaaat
     6841 tgaaataaaa aacccgatgc gcagatcatc gggttcattt caattgagga aatcgggaga
     6901 attacgccac ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt
     6961 tttcgatgtt caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt
     7021 ctgttgaaag cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa
     7081 gcacgtctga aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga
     7141 taactaccgt gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa
     7201 caatcggcac aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg
     7261 caatccgtgt tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt
     7321 actcttcatc accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct
     7381 ttgttacatt cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt
     7441 cgccatacgt tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac
     7501 gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc
     7561 aaggttttta accacaacct ggtgttgacc aattaattga tccaccagca aggcgtagcg
     7621 gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg gcttcggttt tcgcgcccgc
     7681 gacgttgaac actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac
     7741 ccgctcgccg ccggccagtg gatggagatc ggcttcacgg ggttgcagtg attccataac
     7801 agcattcagc ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat
     7861 ggccagcgtc agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat
     7921 ttcgacatga ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg
     7981 cccggagacg tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcattgc
     8041 aacttcgtcg tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag
     8101 aattcgctca cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc
     8161 ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt
     8221 ttctggcagt tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat
     8281 aatgcgttct atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac
     8341 ctgcttgccg agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata
     8401 ttccatcggc atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg
     8461 taactgcccc atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg
     8521 ctgggcaagc atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac
     8581 cttttctacc gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc
     8641 cacgcggccg gttggcgctt gttcggctgc cagtttaagc acaggtgggg tagctatttt
     8701 tggcgaaact tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac
     8761 cgctgtgatg tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc
     8821 tttcaccacg tcatttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc
     8881 gccggccttc aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tttgctcatc
     8941 ttgcggttca cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt
     9001 cgcttctaat gccagttgac gcaaggcctg gcagatataa tcgtagctgg cggcatccgg
     9061 ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa
     9121 cagattgata atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt
     9181 ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt
     9241 gatcgagtgg gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg
     9301 caaaaccagc agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt
     9361 ctgataaaaa tcgcttatat cgatgctcac gctgtcacct cggttcggct gatggcattg
     9421 tgggaacact gacctgtggt gcaacctcag gtttttctag ggcgcttact ggctcattct
     9481 ggctttcggc gttttcatgc aaaatggcct gttcggcttg tttgttcagt accaacaggc
     9541 tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca
     9601 tgccgacgac acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg
     9661 atgcattggc ccgatcggca gaaagctccc agttgctgta acctttctca ccgctggcgt
     9721 agggaaaatc atcggtatga cctgaaaggc taatacggtt gggaataccg ttcagcacag
     9781 gcgcgatggc gcgcagaatg tcgcgcatat agggttcgac atcggcactg ccggttctaa
     9841 acatcgggcg attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga
     9901 ttttgagatg gagacgtaac gcccgcagtt tcggatcgga ctctatcaac tggtcgagat
     9961 caccccgcaa tttccgcaat cgactttgct ccatgcgttt tttcagctct tcgatgttcg
    10021 gctgcttatt cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc
    10081 tttcactatt ggaaatgcga tcgccgcccg taaccgctgt cgccagtgga gtccggaagt
    10141 actccgcaat ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa
    10201 aaaaggccat catcgcagtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg
    10261 ccccgtggct tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca
    10321 tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag
    10381 ttcaataaac gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat
    10441 aggcggtgcg taaccgttca gattagaaag cagagtgact ttgacgcact gcatcatttt
    10501 gctggtttcg gcgcttttct gacgtaaaac actcgctagt ggggaaataa atccgtaagc
    10561 caataaaata ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc
    10621 ggcaggacga tcggctgaac ctaacgcgtg aacgcccccc attacagccg cgacaatacc
    10681 aaacgccgga agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct
    10741 ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc
    10801 gctgataatc aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat
    10861 gcgtgggtag ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga
    10921 aaacatcccc atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat
    10981 atacattgct ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc
    11041 tttaatcgct ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat
    11101 caccagttca gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc
    11161 gaaaactgta ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca
    11221 ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggaaaaaaaa
    11281 agcagcggta cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc
    11341 ctgtactctc tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt
    11401 acgtcttttt actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg
    11461 ctggtgagcg tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa
    11521 tccactttca acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gtggtccttc
    11581 ttctgcttgt gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc
    11641 attacacaaa ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac
    11701 gttttgttcc caggtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg
    11761 gcttccgcgc agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct
    11821 ttccagcatc tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg
    11881 cgcttcctga acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct
    11941 tcaggctgat taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc
    12001 tggagatcgt caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa
    12061 cggaagtgac aaaccagctg attggtttct gccagcttaa ccatttgcgg aag
//
LOCUS       DAZXUL010000121        12105 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZXUL010000121 DAZXUL010000000
VERSION     DAZXUL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.121"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..129)
                     /locus_tag="ACH3L1_003393"
     CDS             complement(<1..129)
                     /locus_tag="ACH3L1_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078012.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDW"
     gene            complement(341..1081)
                     /gene="cysQ"
                     /locus_tag="ACH3L1_003394"
     CDS             complement(341..1081)
                     /gene="cysQ"
                     /locus_tag="ACH3L1_003394"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFT4078013.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            1271..3214
                     /gene="cpdB"
                     /locus_tag="ACH3L1_003395"
     CDS             1271..3214
                     /gene="cpdB"
                     /locus_tag="ACH3L1_003395"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFT4078014.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3340..3720)
                     /locus_tag="ACH3L1_003396"
     CDS             complement(3340..3720)
                     /locus_tag="ACH3L1_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFT4078015.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3809..4669
                     /gene="qorB"
                     /locus_tag="ACH3L1_003397"
     CDS             3809..4669
                     /gene="qorB"
                     /locus_tag="ACH3L1_003397"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFT4078016.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            4778..5743
                     /locus_tag="ACH3L1_003398"
     CDS             4778..5743
                     /locus_tag="ACH3L1_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFT4078017.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5851..6513
                     /gene="ytfE"
                     /locus_tag="ACH3L1_003399"
     CDS             5851..6513
                     /gene="ytfE"
                     /locus_tag="ACH3L1_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFT4078018.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6558..7970)
                     /gene="cycA"
                     /locus_tag="ACH3L1_003400"
                     /gene_synonym="dagA"
     CDS             complement(6558..7970)
                     /gene="cycA"
                     /locus_tag="ACH3L1_003400"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFT4078019.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(8278..8898)
                     /gene="fklB"
                     /locus_tag="ACH3L1_003401"
     CDS             complement(8278..8898)
                     /gene="fklB"
                     /locus_tag="ACH3L1_003401"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFT4078020.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            9117..9662
                     /locus_tag="ACH3L1_003402"
     CDS             9117..9662
                     /locus_tag="ACH3L1_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HFT4078021.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(9646..10293)
                     /locus_tag="ACH3L1_003403"
     CDS             complement(9646..10293)
                     /locus_tag="ACH3L1_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFT4078022.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            10505..11296
                     /locus_tag="ACH3L1_003404"
     CDS             10505..11296
                     /locus_tag="ACH3L1_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFT4078023.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            11306..>12105
                     /locus_tag="ACH3L1_003405"
     CDS             11306..>12105
                     /locus_tag="ACH3L1_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFT4078024.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         3077 a         3117 c         3019 g         2892 t
ORIGIN      
        1 ccaatctttt ttcttaaatg catgctgaat attattaaaa aaatcgtttg aatgtgtttt
       61 tgtatgcgat gactttacaa ataactccac cgcaccaata aaattattgt gtctgatcct
      121 gggtaacata tggctacctt attttatttg gttattttat gtcgccatta aatatctcat
      181 tatagaagta aactatcaaa aaacgctcat tattaaaatg taacttacta gcaattgaat
      241 ttttgcactt taaatacaat ctctttttat ttataacttt ttaggttggg ataaatcagg
      301 atggcagaat caggaaatac actgtttctg ccatctgaat ttagtaaatg gacactctga
      361 accccggatt caggaacgac tcgcgcggag tgtaatccag cggtttaccc tgccagtcgt
      421 gaacgtgcgc tccggcagct gcagccacag catgtccggc ggcggtgtcc caaatattcg
      481 ttggtccgaa gcgcgggtac agctgcgcct gtccttccgc caccaggcag aatttcagcg
      541 aagagccgat ggacgtggtc tgatgttcgc caagctgttg cagatactct ttcagctccg
      601 catctgcatg ggaacggctg ataaccacca gcggcgggcg cgcatcgcgg acctgaatca
      661 gcttgcgcac accgcactct tctttccagg ccttgccttc tgccgcgctg tacattacgt
      721 tcattaccgg cgcatacacc acacctaaaa tcggtttgcc atggtcaatg agcgcaatgt
      781 taacggtgaa ttcgccatta cgtttaataa actctttagt accatccagc gggtctacca
      841 gccagtaacg ctgccagtgc tgacggactt cccaaccggg aggatcttct tcagaaagga
      901 ccggaatatc cggtgccagc gtacgtaaac cgtccatgat aacggtgtga gcggcaatat
      961 ccgctgccgt taccggagaa ttgtccgctt tgctgacgac gtccatcggt ttcgtcccgt
     1021 cgtagacctg cataatggca tcgcctgcat tccgtgcaag ctggcatact tgatctaaca
     1081 tttctccacc tcgtctctgt gagcggtgtt aacttattgt tttacttata ccctatcgtt
     1141 aatgaatgcg ccaactgtga tagtgtcatc attttcaaag cgtaaaattg tggcattctt
     1201 cactgttcta taagtaaggc gtttattctc ccttttcttt cgtattcccg atgataaaag
     1261 gatgtccctg atgattaagt ttagcgcaac gctcctggcc acgctgattg ccgccagtgt
     1321 gaatgcagcg acggtcgatc tgcgtatcat ggaaaccact gatctgcata gcaacatgat
     1381 ggatttcgat tattacaaag acaccgccac ggaaaaattc ggactggtac gtacggcaag
     1441 cctgattaac gatgcccgca atgaagtgaa aaacagcgta ctggtcgata acggcgattt
     1501 gattcagggg agtccgctgg ccgattacat gtcggcgaaa ggattaaaag caggtgatgt
     1561 tcatccggtt tataaggcgc tgaatacgct ggattatacg gtcggtacac tcggcaatca
     1621 tgaatttaac tacggtctgg attacctgaa aaatgcgctg gcgggagcga aattccctta
     1681 tgtaaatgcc aacgtcattg acgccagaac caaacagcca atgtttacac cgtatttaat
     1741 taaagatacc gaagtggtcg ataaagacgg aaaaaaacag acgctgaaga ttggctatat
     1801 tggcgtcgtg ccgccgcaaa tcatgggctg ggataaagct aatttatccg gaaaagtgac
     1861 ggtgaatgat attaccgaaa ccgtgcgcaa atacgtgcct gaaatgcgcg agaaaggtgc
     1921 cgatgtcatt gtcgttctgg cgcattccgg gctgtctgcc gatccgtata aagtaatggc
     1981 ggaaaactca gtttattacc tcagtgaaat tccgggcgtt aacgccatta tgtttggcca
     2041 tgctcacgcc gttttcccgg gtaaagattt tgctgatatc gaaggggctg atatcaccaa
     2101 aggcacgctg aatggtgttc cggcggtaat gccgggcatg tggggcgatc atcttggggt
     2161 ggtcgactta caactcagta atgacagcgg taaatggcag gtgacgcagg cgaaagcgga
     2221 agcacggccg atttacgaca tcgccaataa aaaatccctc gcggcggaag acagcaagct
     2281 ggtagaaaca ctcaaagccg atcacgatgc cacacgccag ttcgtcagca agccaatcgg
     2341 taaatctgcc gacaatatgt atagctatct ggcgctggtg caggacgatc cgaccgtgca
     2401 agtcgtgaac aacgcgcaaa aagcgtatgt cgaacattac attcagggcg atccggatct
     2461 ggcaaaactg ccggtgcttt cagctgccgc accgtttaaa gttggtggtc gcaaaaatga
     2521 cccggcaagc tatgtggagg tggaaaaagg ccagttgacc ttccgtaatg ccgccgatct
     2581 ttatctctac cccaatacgc tgattgtagt gaaagccagc ggtaaagagg tgaaagagtg
     2641 gctggagtgc tccgccggac agtttaacca gattgctcct aacagcacga aaccacagtc
     2701 actcatcaac tgggatggtt tccgcactta taactttgat gttattgatg gtgtgaatta
     2761 tcagattgat gttacccaac ccgcccgcta tgacggcgag tgccagatga ttaatgccaa
     2821 tgcagagcgg attaagaacc tgacctttaa tggcaagccg attgatccga acgctatgtt
     2881 cctggttgcc accaataact atcgcgctta cggcggcaaa tttgccggta cgggcgacag
     2941 ccatatcgct tttgcttcac cggatgagaa ccgctcggtg ctggcagcgt ggattgctga
     3001 tgagtcgaaa cgtgcggggg aaattcatcc ggcggcagat aacaactggc gtttagcacc
     3061 gatagctgcc gataagaaac tggatatccg tttcgaaact tccccgtcag ataaagccgc
     3121 agcgtttatt aaagagaaag ggcagtatcc gatgaataaa gtcgcgaccg atgatatcgg
     3181 gtttgcgatt tatcaggtgg atttgagtaa gtaaaacact tctttttcgg cctataaatc
     3241 aaccgcatcc ggcatttatt ggcggatgcg atgctgccgt atcttatcca ccctacaagc
     3301 catgaaccgt agaccggata agcgcagcgc atccggcagt tatgccgcac gttcatcccg
     3361 caccgccaac acctcgggca aattcaactc aatccagtcc gccagtgcag caaccttttc
     3421 gctcacctgc tcgcccagcg gcgtgaggct atattccaca tgcggcggca ccaccggata
     3481 cgcgatacgg ttaagaaaac catcctgttc taacgcctgt aacgactgcg caagcatctt
     3541 ttcactcacc ccaccgattt tgcgccgcag gtcgctaaag cgatgagtac cttcgcgtag
     3601 cgccaccaga atcaacaccc cccaacggct ggtgacgtgt ttcaacacct cgcgcgacgg
     3661 gcactgttcc gcaaagagat taccctcttt cagttgttgt gacaggctaa cctgactcat
     3721 ttcatactta cctttttgca cgtacttact aaaagtaagt ttaggtgtta gcgtatttaa
     3781 acacaagaca aaacgatgga gacttcccat gatcgctatt actggtgcca ctggccaact
     3841 tggtcactat attattgaat ccttgatgaa aacggttcct gccagccaaa tagtggctat
     3901 cgttcgtaat ccggcaaaag cccaggccct ggcagcacaa ggcattaccg tgcgtcaggc
     3961 tgactacagc gatgaagccg cactgacatc tgcacttcag ggagtggaaa aactactgct
     4021 gatctcttcc agcgaagtgg gtcaacgtgc cccgcagcat cgtaatgtta ttaatgccgc
     4081 aaaggcggct ggcgtgaaat ttatcgctta taccagcctg ctacatgcag atacctcccc
     4141 gctcggcctc gccgatgagc acatcgagac ggagaaaatg ctggctgatt ctggcatcgt
     4201 ttacaccctg ctgcgcaacg gctggtacag cgaaaactac ctcgccagcg ccccggcagc
     4261 actggaacac ggcgtattta tcggtgcggc tggcgatggc aaaatcgcct cagcaacgcg
     4321 ggcagattat gcgacagctg cggcacgcgt gattagcgaa gccggtcacg aaggcaaggt
     4381 ttacgaactg gcgggcgata gtgcctggac gttgacacag ttagcggcag agctgaccaa
     4441 acagagcggc aaacaggtta cctatcaaaa tctgagcgaa gccgatttcg ccgcggcact
     4501 gaaaagcgtc ggactgcccg acggactggc ggatatgctg gcggattctg acgttggcgc
     4561 atcgaaaggt ggtctgtttg ataacagcaa aacgcttagc aaattgattg gctgcccaac
     4621 gacaacgtta gccgaaagcg taagccatct ttttaatgtt aataactagt taattaaagt
     4681 ggcatcctcc cgcatcctct ctgataatga cgggatgccg ggagcaatca tgtctgcttc
     4741 ctgaacgttt cttctgacag accaatggat gccagtaatg attagcggcg tgctgtacgc
     4801 cctgttagca gggttgatgt gggggcttat ttttgtcggg ccgttgatcg tgccggaata
     4861 cccggcgatg ttgcagtcga tggggcgtta tctggcgtta gggttaattg cgctacccat
     4921 tgcctggctg ggacgcgtgc gtctgcgtca gttggcgcgt cgggactggc ttaccgcctt
     4981 gatgctcact atgatgggca acctcattta ttacttctgc cttgccagtg ccattcaacg
     5041 tactggcgcg cctgtttcca cgatgattat cggcaccctg ccggtggtga ttcctgtctt
     5101 tgccaatctg ctttatagcc agcgcgacgg caaactcgcg tggggaaaac tcgccccggc
     5161 actgatttgt attggcatcg gcctggcgag tgtgaatatt gctgagttaa accacggact
     5221 ccccgatttt gactgggcac gttatacctc tggcatcgtg ctagcgttag tttccgtggt
     5281 ctgctgggca tggtatgccc tgcgcaacgc ccgctggctg cgggaaaatc ccgacaaaca
     5341 tccgatgatg tgggcgacgg cgcaggcgct ggtcacgctg ccggtttctc tcatcggcta
     5401 tctcgtcgcc tgttactggc tgaatataca aacgccggac ttctccttac cttttggccc
     5461 ccgtccgctg gtgtttatta gtctgatggt tgcgatagcc gtgctttgct catgggttgg
     5521 cgcactctgc tggaacgtcg ccagccagcg attaccgaca gtgattctcg ggccgctgat
     5581 tgttttcgaa acgctggcag gtttgctgta caccttttta ctccgccagc aaatgccgcc
     5641 gctaatgacg ctgagcggta tcgcgctgtt agtgattggc gtggtcatcg cggtcagagc
     5701 aaaaccagaa aaacctttaa ctgaatctgt ctcagaaagt tgacatgctg gcagtgagtt
     5761 aaataagcct ctgctacgta agggttatag cttttgcctt aaaggtgtat ttaaaataca
     5821 tcttatctta ttaagaatga ggtatcagct atggcttatc gcgaccaacc tttaggtgaa
     5881 ctggcgctct ctatccctcg cgcttcggct ctgtttcgta aatatgatat ggattactgc
     5941 tgtggcggta agcagacgct ggcgcgcgcg gcggcacgta aagaactgga tgttgaggtc
     6001 attgaagctg aactggcaaa gcttgctgaa caaccgattg agaaagactg gcgtagcgcc
     6061 ccgctggcag aaatcattga ccatatcatc gtgcgctacc acgatcgtca ccgcgagcag
     6121 ctgccggagc tgatcctgca agcgactaaa gtcgagcgcg ttcacgccga caaaccgagc
     6181 gtgccaaaag ggctgacaaa atacctgacc atgctgcatg aagagctttc cagccacatg
     6241 atgaaagaag agcagatcct cttcccgatg atcaaacaag gcatgggcag ccaggcaatg
     6301 gggccaatca gcgtaatgga aagcgagcac gatgaagcgg gcgaactgct ggaagtgatc
     6361 aaacacacta ccaataacgt cacaccgccg ccagaagcct gcaccacctg gaaagcgatg
     6421 tataacggca ttaatgaact gattgatgac ctgatggatc acatcagtct ggaaaacaat
     6481 gtactgttcc cacgcgccct agcgggtgag tgatctaaaa gctggatggc attgcgccat
     6541 ccagcatgat aatgcggtta tttccgcagt tcagcagccc gcttcttacc aataaacagc
     6601 cagcccaacc ccagcgcgat aaaccacagc ggggtaacca gcagcgcctg gcgagtgtca
     6661 tcttccagtg tcagcaacac gaccacgaac acaaagaacg ccatacatac ccagcacatc
     6721 agcttgccga gcggcatctt gtagatcgac ttttcatgca gatgaggacg ctgtttgcgg
     6781 tacaccaggt aagagcaaag gataatcgtc cagacgaaca taaacagaat cgcggaaacg
     6841 gttgtaatca tcgtgaacgc gccaatcacg ctaggattca catacaacat caccacgccg
     6901 ccgagcagac agatacacga gaaggtcaac cctttcgcgg gtactgcgcg cttagaaagt
     6961 ttagcgaacg ctttcggcgc aacaccttcc tgcgccagac caaacagcat acggctggta
     7021 gagaagacac cgctgttagc ggaagacgcc gcagaggtca gcaccacaaa gttgatcacg
     7081 ctggcagccg caggcaaacc taccaacaca aacagctcaa cgaacgggct tttctccggg
     7141 actaccgaac tccacggtgt cacggacata atcacaatca gcgcgaagac gtagaacata
     7201 atgatacgga tcggaatgga gttaatcgcg cgtggcagtg atttctccgg atctttggtt
     7261 tccgcagctg ttgtacctac cagctcaatc cccacgaaag cgaaaaccgc tatctggaat
     7321 ccggcaaaga agccacttaa acctttcggg aaccagccgc cgtcattcca caaatgcgcg
     7381 aatgacgctt ccacaccggt cggtgactga aagtgcatcg ccaccatgac caggccgacg
     7441 acaatcagcg acacgatggc gacgattttg atcatcgcaa accagaactc catctcaccg
     7501 aacattttca cggtggcaag gttgagcgtc agcagcagca ctatcaccgc cagcgaggcg
     7561 acccagtcgg agagatcggg gaaccagaac tgagcataag ccgtgatcgc caccacgtct
     7621 gccataccgg ttacaaccca gcagaaccag taagtccagc cggtgaaata tcccgcccac
     7681 ggccccagta aatcggaagc gaagtcacta aaagatttgt attccagatt cgaaagcagc
     7741 aattccccca ttgcccgcat cacgaaaaag agcataaaac caatgatcat ataaacgaaa
     7801 atgatcgacg gcccggcaag gctaatcgtt ttgccggacc ccataaacaa cccagtacca
     7861 atggcacctc caatggcaat aagctgaaca tgtcggtttg tgagattgcg ccgtagcgac
     7921 tgttcagccg gagcctgatc atcggcaacg acttttacct gatctaccat gttttttctt
     7981 cctgtacctg tctgtgttgt tcaggctcta cggcctttag tcagtctatg atacgacgaa
     8041 tctgtatcgg gactcatcga tattaggtaa caatacgcgg gatgaatact aagatttaga
     8101 tctaatgtta attttatgtt taaagtgagt gtcatatcac ggtgataacg cgaaacagct
     8161 cacaaaaata cacgcttacg gggtctttgc aacacaaaat agcgccgttt ggcaaactga
     8221 agggtttatt gctgaatgcc tgctcccctc tggttcgagg ggaacagaat gcgctgctta
     8281 gaggatttcc agcagttcga cttcaaacac cagggtgctg aacggaggga tggatgcacc
     8341 tgcgccgcgc tcgccatatg ccagttcctg cgggatagtc agttcccatt tagaacctac
     8401 cggcatcaga gtcagtgctt caatccagcc agggatcacg ccattaaccg ggaattcagc
     8461 gggttcacca cgagcaacgg agctgtcaaa cacggtgccg tcgatcagtt taccggtgta
     8521 atgaacacga acgcggtcgg tacgtgccgg aattgcgcct tcaccctggt tgatcacgcg
     8581 gaattgcagg ccagattcgg tgctattcac accttctttt ttggcgtttt cttccaggta
     8641 tttcacgcct tcagcagcca tcgcctggaa acgctgacga cgaacggcat cggcgcgttc
     8701 gtggatttca cgcagcgcgc gatgcaccac atcaaccggc acagccggat gtttgccttc
     8761 cagcgcatcg gcaatacctg caaccagtgc ttctggcagc agcccttcca ggccagattc
     8821 actcagttgt tgccctacct gcaaaccaat gccgtagctt gcttgcgctt cgatggtgtc
     8881 aaaagttggg gtggtcatcg tatttccttt cttagggtat aaaagtagcg ggcagcatat
     8941 cagccacact tctttgggta aaactttgtc gcaggaatga tgacaaatcc cggggaaaaa
     9001 gaaacaatag caatatcccg tatgaatata ataaaggcgg gtttaccacg gacatcatag
     9061 cgttagctta ttcataggct atgattgagg aacaagacgt ggagcaggag gaaaccatgc
     9121 ccgggcgctt tgaactaaaa ccaaccctgg agaaagtctg gcacgcgccg gacaattttc
     9181 gcttcatgga cccgctgccg ccgatgcatc gtcgggggat cattattgcc gccattgtat
     9241 tggtggtcgg atttctgctt ccatctgatg atacgcccaa cgcgtcggtg gtgacgcgtg
     9301 aagcgcagtt agatattcag tcgcaatcac aaccgccgac agaagaacag ccgcaaacta
     9361 cccaaacgca accattccag ccagatagcg gaattgataa tcaatggcgc tcttaccgtg
     9421 tcgaacccgg taaaacgatg gcgcagctgt tccgcgacca cggactgccc gctaccgatg
     9481 tctatgcaat ggcgcaggta gaaggcgcgg gtaagccgct gagtaatttg caaaatgggc
     9541 agatggtgaa aatccgccag aacgccagcg gcgtggtgac gggcttaacc attgatacgg
     9601 ggaataatca acaggtcctg tttacccgcc agccggatgg cagttttatt cgtgcgcggt
     9661 agatgaaggg tcaaccaaaa gccagctaag gattgtctct ttgatgacct tacgtctggc
     9721 ttccagtgcc tggggggaaa ccatactgat atgataaact aggccaagcg tatgctggtt
     9781 gatcaaataa tatcccccaa gcgcagcaat attaatattc acctgcaatg ggtcgatacc
     9841 tggcttaaaa atattgtatt tcttcccttc atccaataag gattccatta actgcaagtg
     9901 agcatggtta atttctaaga gacgctgtga ttttgcataa tgcacacctt tactttgatt
     9961 ttcactatgc acaattttta aaaaccaagg attagcaata taataatccc atgtaaaatc
    10021 aataattttc tcaatggctt tttgtggcgg aagatctgag aaatcaaggg ccttttcttt
    10081 ttgtcgaata tcgttccagg tatattccag cacttcgata aataaatttt ctttattacc
    10141 aaagtggtga taaaccgtct gtttactaca ccctgctgcc tttacaatat tatctactct
    10201 ggccccttca taaccatatt cggcaaattc attaaccgca catagcagca atttttcctt
    10261 caggctctgt acgttgctgg tatcaatata cataacgctt ccccacgacc tcttcgtggt
    10321 gactatcata acctgatttg atttcagctt cagaaaaaag ggcaaaaact tgtactttca
    10381 tcaggttatc aaatgtcctg tgatcgccat cgcatatcaa ccaactagat ggtttatctt
    10441 tttatcctcc agtcgcaagc taatcatcac caaggaaaca aaattcaaaa aagagagcta
    10501 ttgtatgaaa tccactcgtc ctgttgccgt gattaccgga gccactcgcg gtattggaaa
    10561 gggctgtgcg caggagcttg ctcgtggcgg gtttaacctg ctgattaacg atcgtcccga
    10621 tgccgacagc gtagaaaaac tgcatatcac acaacaagaa tgtttggcgg aaggcgtgga
    10681 agtgatttgc ttccctgctg atgtcggtga tctatctctg catgaggaga tgctcgacgc
    10741 ggcgcaaaac cagtgggggc ggctggactg tctgctcaat aacgcgggga tttcagtgaa
    10801 aaaacgcggt gacctcctcg acctcgaacc agacagcttc gatcaaaaca ttgccattaa
    10861 cacccgcgcg ccgttcttcc ttgctcaggc attcagtaag cgcttactgg cacagccgaa
    10921 accggaagcg gagctgccgc accgctcgat tattttcgtc agttccatca acgccatcat
    10981 gctggcgatg aatcgcggcg aatacaccat tgccaaaacc gccgtttctg ccgctgctcg
    11041 tctgtttgct gctcgccttt gtaatgaaca aattggcgtt tatgaagtgc gccctggtct
    11101 catcaaaact gatatgacta ttccggcaac ggcttattac gacgaactga ttgccaaagg
    11161 tctggtgccg tggggacgtt ggggttatcc ggctgatatt gcttccaccg tccgcgcgat
    11221 ggcggaaggt aaactgattt acacctgtgg tcaggcggtc gcaatcgacg gcggcttaag
    11281 tatgccgcgc ttctgaggct gaatcatgag agatctgtta caacgcccgg atttattcag
    11341 tatcaatacc gcgacactgg gctataaaac gccgctaccc gccattattg atgcctgcgc
    11401 cgcacgcggt attggcgcaa ttgccccctg gcgcagggag ttgcagggtg aagatctcca
    11461 gcaaatcacc cgccagcttg ccgccagtaa catgagcgtt tccggtttgt gccgtagcac
    11521 ttactacacc gcgccgacac tggcggaacg caaactggcg attgatgata accgccgggc
    11581 gctggacgac gcagcggtgc ttaacgccgc ctgttatatg caggtggttg gcggtctgcc
    11641 aacgggcact aaagatctat atgaagcgcg agaacaagtg aaacaaggga ttcgccagtt
    11701 gctgccacat tcgaaagatg tcggcgtgcc aattgcgctg gagccactgc acccgatgac
    11761 agctgccgat cgctcttgcc tgtgcacgtt acgtcaggcg ctggactggt gtgatgaact
    11821 cgatcccgat ggtgagtttg gcctcggcgt ggcggtggat gtttatcacg tctggtggga
    11881 tccggatctc gccagtcaga tcctgcgtgc cggaaaacgc atcctcgcgt ttcatgtttc
    11941 cgactggtta atgccgacca ctgatctggt caatgaccgt ggtatgccgg gagatggcgt
    12001 catcaatatt ccgtcaattc gtcggctggt tgaaaacgcc ggatttaatg gtgctattga
    12061 actggaaata ttctcgcctt actggtggca gaaagatatt aacag
//
LOCUS       DAZXUL010000122        12034 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZXUL010000122 DAZXUL010000000
VERSION     DAZXUL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12034
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.122"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..248)
                     /gene="yefM"
                     /locus_tag="ACH3L1_003406"
     CDS             complement(<1..248)
                     /gene="yefM"
                     /locus_tag="ACH3L1_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFT4078025.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     misc_feature    548..671
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            718..1617
                     /gene="hisG"
                     /locus_tag="ACH3L1_003407"
     CDS             718..1617
                     /gene="hisG"
                     /locus_tag="ACH3L1_003407"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFT4078026.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1623..2927
                     /gene="hisD"
                     /locus_tag="ACH3L1_003408"
     CDS             1623..2927
                     /gene="hisD"
                     /locus_tag="ACH3L1_003408"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFT4078027.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2924..3994
                     /gene="hisC"
                     /locus_tag="ACH3L1_003409"
     CDS             2924..3994
                     /gene="hisC"
                     /locus_tag="ACH3L1_003409"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFT4078028.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3994..5061
                     /gene="hisB"
                     /locus_tag="ACH3L1_003410"
     CDS             3994..5061
                     /gene="hisB"
                     /locus_tag="ACH3L1_003410"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFT4078029.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5061..5651
                     /gene="hisH"
                     /locus_tag="ACH3L1_003411"
     CDS             5061..5651
                     /gene="hisH"
                     /locus_tag="ACH3L1_003411"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFT4078030.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5651..6388
                     /gene="hisA"
                     /locus_tag="ACH3L1_003412"
     CDS             5651..6388
                     /gene="hisA"
                     /locus_tag="ACH3L1_003412"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFT4078031.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6370..7146
                     /gene="hisF"
                     /locus_tag="ACH3L1_003413"
     CDS             6370..7146
                     /gene="hisF"
                     /locus_tag="ACH3L1_003413"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFT4078032.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7140..7751
                     /gene="hisIE"
                     /locus_tag="ACH3L1_003414"
     CDS             7140..7751
                     /gene="hisIE"
                     /locus_tag="ACH3L1_003414"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFT4078033.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="ACH3L1_003415"
     CDS             complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="ACH3L1_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFT4078034.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="ACH3L1_003416"
                     /pseudo
     CDS             complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="ACH3L1_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="ACH3L1_003417"
     CDS             complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="ACH3L1_003417"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFT4078035.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         3011 a         3062 c         3048 g         2913 t
ORIGIN      
        1 tcaataatgt ccttttccgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
       61 ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga attgtattct
      121 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taatgaggat cggggcatga
      181 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
      241 gtacgcatgt aaatctccta ttttgtacag ttcattgtac aatgataggt gttaattaac
      301 tatttattaa ttagtttgta gatcaagaca ttgccagcga gacgaaaaat cagactttgc
      361 tctttttggt gctgtcaagt tagaggacag tcctcttagc cccctccttt ccccgctcat
      421 tcattaaaca aatccatcgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
      481 gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagattttt atgacacgcg
      541 ttcaatttaa acaccaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
      601 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
      661 ggctttttta ttgcgcggtt gttaacggtt cagacaggtt taaagaggaa taagaaaatg
      721 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
      781 cgcgaattac tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg
      841 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgatgacat tccctgtctg
      901 gtaatggacg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
      961 cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccctgcgtcg tctggatttc
     1021 ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc
     1081 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
     1141 aaaggtatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc gccgcgtgcc
     1201 gggctggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc
     1261 ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa
     1321 atggaagaat ctaaacagca actgatcgac aagctactga cccgtattca gggtgtgatc
     1381 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc
     1441 atcgccctgc tgccaggtgc cgaacgccca accattctgc cgctggcggg tgatcaacag
     1501 cgcgtagcga tgcacatggt gagtagcgaa accctgttct gggaaacgat ggaaaaactg
     1561 aaagcactgg gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg
     1621 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
     1681 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt aacgatattc
     1741 tcgataacgt gaaaacgcgt ggcgatgagg ccctgcgtga atacagcgcg aagtttgata
     1801 aaaccacggt taccgcgctg aaggtgtctg ctgatgagat cgccgccgcc agcgaacgcc
     1861 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gagtattgaa accttccaca
     1921 ctgcgcaaaa actgccgccg gtagatgtag aaacgcagct aggcgtgcgt tgccagcagg
     1981 tcacgcgccc ggtggcttca gtgggtttgt atattccggg cggctccgcc ccgctcttct
     2041 caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
     2101 gctcaccgcc gccgattgcc gatgaaattc tttatgcggc gcagctgtgc gatgtgcagg
     2161 acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg
     2221 tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc
     2281 aggtaagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc
     2341 tggtgattgc tgacagcggc gctacgccgg attttgtggc ttctgatttg ctttctcagg
     2401 ctgaacacgg ccctgactca caggtaattt tactgacccc cgatgctgat atggcgcgtc
     2461 gcgttgccga ggccgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accacccgcc
     2521 aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtggagatct
     2581 ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgc gaactggtcg
     2641 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg
     2701 actacgcctc cggcaccaac cacgtgctgc cgacttacgg ttacaccgcc acctgttcca
     2761 gcctcgggct ggcagatttc cagaaacgca tgaccgtaca ggaactgtcg aaagaaggtt
     2821 tctccgcgct ggcttcgacc attgaaacac tggccgccgc cgagcgcctg accgcccaca
     2881 aaaatgccgt tactttgcgt gttaacgtcc ttaaggagca agcatgagca ccgtgactat
     2941 tactgattta gcgcgtgaaa acgtccgcaa cctgacaccg tatcagtcgg cgcgtcgtct
     3001 gggcggtaac ggcgacgtct ggctgaacgc caacgaatat cccacagccg tggagtttaa
     3061 gcttactcag caaacgctca accgctaccc ggaatgccag ccaaaagcgg tgattgaaaa
     3121 ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg gcgcggacga
     3181 aggtattgaa ctgctgatcc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
     3241 cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggtgtcg aatgccgcac
     3301 agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca agctggacgg
     3361 cgtaaaagtg gtctatgttt gcagccccaa caaccccacc gggcaactga tcaatccgca
     3421 ggattttcgc accctgctgg agctaacccg cggtaaggcg attgtggttg ccgatgaagc
     3481 ttatatcgag ttttgcccgc aggcatcgtt agctggctgg ctggcggaat atccgcacct
     3541 ggcgattttg cgtacccttt cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac
     3601 gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atccccccct acccgctctc
     3661 gacgccggtt gccgacattg ccgcacaggc gttaagcccg cagggaatcg tcgccatgag
     3721 cgaacgggtg gcgcaaatta ttacagaacg cgaatacctg attgccgcac tgaaagagat
     3781 cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc gctttaaagc
     3841 ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
     3901 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
     3961 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
     4021 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
     4081 ctcgcctttg aaccgggcgt gatcccggaa ttgctgaagc tgcaaaaagc gggctacaag
     4141 ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca agcggatttt
     4201 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa
     4261 gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa
     4321 ctggtggagg gttatctggc tgagcaagcg atggatcgtg ccaacagtta tgtaattggc
     4381 gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac
     4441 cgcgaaaccc tgaactggcc aatgattggc gagcaactca ctaaacgaga ccgttacgcc
     4501 catgtagtgc gcaacaccaa agagacgcag attgacgtcc aggtgtggct ggatcgcgaa
     4561 ggtggcagca agattaacac cggcgttggc ttctttgatc acatgctgga tcagatcgcc
     4621 acccacggcg gtttccgcat ggaaatcaat gtcaaaggcg acctctatat cgacgatcac
     4681 cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac
     4741 aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc
     4801 gcactggata tctctggtcg cccgcacctg gaatataaag ccgagtttac ctaccagcgt
     4861 gtgggcgatc tcagcactga aatgatcgaa cacttcttcc gttcgctctc ttacaccatg
     4921 ggcgtgacac tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agaaagcctg
     4981 ttcaaagcct ttggtcgtac cctgcgccag gccatccgcg tcgaaggcga caccctgccc
     5041 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga tactggctgc gccaacctga
     5101 actctgtgaa gtctgccatt gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg
     5161 acattgtgtt gctggccgat aaactgtttt tacccggcgt cggcaccgca caagcagcga
     5221 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
     5281 tgggcatctg cttagggatg caactcctgg gacgacgcag cgaagagagc aatggcgtcg
     5341 acttgctggg catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctgccactgc
     5401 cacatatggg ctggaaccgc gtttatccgc aggcaggcaa ccgcctgttt caggggattg
     5461 aagacggcgc gtacttttac tttgttcaca gctacgcaat gccagtcaat ccgtggacca
     5521 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
     5581 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc
     5641 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
     5701 tcagggcgat tacggcaaac aacgcgatta cggtaacgac ccgctgccgc gcttacagga
     5761 ttacgctgcg caaggtgccg aagtgttgca cctggtggat ctgaccgggg caaaagatcc
     5821 ggctaagcgt caaatcccgc tgattaaaac cctggtcgcg ggcgttaacg tcccggtgca
     5881 ggttggcggc ggtgttcgta gtgaagaaga cgtggcggcg ttactggaag ctggcgttgc
     5941 gcgcgtggtg gtcggttcca ccgcagtgaa atcaccggac gtagtgaaag gctggtttga
     6001 acgtttcggt gccgatgcgc tggtgctggc actggatgtc cgtattgacg agcaaggcaa
     6061 caagcaagtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt
     6121 ggaaacctat ctgcccgtcg gcctgaaaca tgtgttgtgt accgatatct cgcgcgacgg
     6181 cacgctggcg ggatctaacg tctctttata tgaagaagtg tgtgccagat atccgcaagt
     6241 ggcgtttcag tcctccggcg gcattggcga cattaacgat gttgctgccc tacgtggcac
     6301 tggcgtgcgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca ccgtgaagga
     6361 ggccatcgca tgctggcaaa acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg
     6421 gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc actggcaaaa
     6481 cgctacgccg aagaaggtgc agatgaactg gtgttctacg atatcaccgc ttccagcgat
     6541 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt
     6601 tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
     6661 gataaaattt ccatcaactc ccctgcgctg gcagacccaa cattaattac tcgcctggcc
     6721 gatcgctttg gcgtgcagtg tattgtggtt ggtattgata cctggtacga cgccgaaacc
     6781 ggtaaatatc atgtaaatca atataccggc gatgaaagcc gcacccgcgt cactcaatgg
     6841 gaaacgctcg actgggtaga ggaggtgcaa aaacgcggcg ccggagaaat cgtcctcaac
     6901 atgatgaatc aggacggcgt gtgtaacggt tacgacctca agcagctgaa aaaagtgcgt
     6961 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctg
     7021 gaagccttcc gcgatgccga cgttgacggt gcgctggcag cttccgtatt ccacaaacaa
     7081 ataatcaata ttggcgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
     7141 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacggt ctgatgccgg
     7201 tgattgtgca acacgcggta tctggcgaag tgttaatgct gggctacatg aacccggaag
     7261 ccttagacaa aaccatcgaa agcggtaaag tcaccttctt ctcgcgcact aaacagcgac
     7321 tgtggatcaa aggcgaaacc tcgggcaatt ttctcaatgt ggtaagtatt gccccggact
     7381 gcgacaacga cacattactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca
     7441 ccagtagctg cttcggcaac accgctcacc agtggctgtt cctgtatcaa ctggaacaac
     7501 tgctcgccga acgtaaatat gccgacccgg aaacctccta caccgccaaa ctgtatgcca
     7561 gcggcactaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag
     7621 caacggtaca tgatcgcttt gagctgacca acgaggcatc tgacttgatg tatcacctgc
     7681 tggtgctgtt gcaggatcag gatctggatt taacgacagt aattgagaac ctgcataaac
     7741 ggcatcagtg agttacgggc tgagcaggtg tgatgttgtt gtcacacccg gagccattgc
     7801 ccgatgcgca aacttatcgt gcctacacat tatctcaaca cacgtaggcc ggataagatg
     7861 cgccagcatc gcatccggca aaaaaaacgg gcatggtgtc accaccctgc ccgtttctct
     7921 taaatgcaca ataatattac ttcgcgttgt aattacgcag agcgttacgc cccaacacaa
     7981 tccccgcgcc aaccatgccg cccagaagca ctgccagaat caaggtgatt gcctttttcg
     8041 gactatcgcg acgaataggt aacgtcggtt tcatcacata gcggtaagca tgaatatcaa
     8101 gatcatcaaa ctttaatttt tcaatatcca acaggttttg acgtgtctga tagtagtttg
     8161 gtgagaacac caacggacga gtcgcttcat gcttaatcat cgactccagc gcttcgctcc
     8221 ctagaaggaa caacgtatct tgcgtcacat cttcagtctg ctgaacctgt ggctttgtca
     8281 cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc agatctttct
     8341 gctcctgcgc gaccacttcc tgggttctta aagagtcctg caagtttttc cgtcccagag
     8401 caatgttgtc cttgagatcc ttttctagct cttgattcac tttatcatca acttgctgaa
     8461 tgtattgggc caacttcatt tgtgcgccct ctgcagtttg cccaacataa gaaacagtca
     8521 atggtaattg ctggttctta acagaaggtt cgatggtaag tttttctggc tcttcctgat
     8581 tatccagcgt ttctgctaat gcagagaagg cagaactgaa gcgaccaatt aacgtctcct
     8641 gcaaatccga tactttcggt gcagcctgac catagataac attcatggca ttgttatagc
     8701 cagcaatttg ccccacgtcg ggctgagtga taattgctgt tgacgtccat ttctccttcg
     8761 ctactgccaa ataaccaata gccagggcaa tagccacaat gacggaaatg ataattgtca
     8821 tcttgccacg ccacaactgc actagtaaat caatcaaatc aatctgttcc gggtcatggt
     8881 tttgcccaga aacattatta ttttctactc tcatcattac cctaactgac ggaaatttcc
     8941 aagtccgcat agtttagcga taatccccgt gaatgctata ggataagtga taaaaataat
     9001 gaattattgc ggaggtaaca aaagacaatc agggcgtaaa tagccctgat aacaagatgt
     9061 tagtcgctgc caaagagatc gcgggtgtag accttatctg ccacatcctt aagctcttct
     9121 gccatacggt tggagataat gacgtcggct tgttgtttga aggtggcgag atcacgttcc
     9181 aggcgagagt tgaagaatga gtcttctttc atcaccggct cgtagatgat cacttcaacg
     9241 cctttcgcct tgatacgctt cataatcccc tgaatagaag acgcacggaa gttatctgac
     9301 ccgctcttca taatcagacg ataaataccc accacttgcg gtttgcatcg acaattgccg
     9361 agatcaggtt attcggcaca gactggtagt tcgccagtaa ctgcttggta tctttcggca
     9421 gacaataacc accataacca aacgacggat tgttgtaatg gttgccaata cgcggatcga
     9481 gacaaacgcc ttcgataatc tggcgagtat tcagacctaa actttctgca taactatcca
     9541 gttcattaaa gtatgctacg cgcatcgcca gataggtatt agcgaaaagt ttaatcgctt
     9601 ctgcttcagt ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta
     9661 acaacgcagc aaaacgttct gcgcgttctg aacgctcacc gatgacaata cgtgacggat
     9721 ggagattatc gtaaagggct ttaccttcgc ggaggaattc cggagagaaa ataatatttt
     9781 cagtacgata tttcttatgc atcgctgcgg taaaaccaac gggaaccgtt gatttgatga
     9841 ccataaccgc ataaggattt atctcaacta cgtctttaat tactgattct acactggatg
     9901 tattgaaata attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag
     9961 catcccggta ggcttcattt ttatctaatg tggcattaaa gtgtatttta tctgattgca
    10021 aaaactgctg aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc
    10081 gtgacggtaa aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg
    10141 acaagcctac atagccagta ccggaaatgg tgattttcat ttcatgctct cagaattaac
    10201 ttaactgtga atcatgatgt ttttagcatc gtgataagat gctaaaagtt ttaacgctac
    10261 gttgtcagat ggtcaacgca acgaaaaaca attcagattt tctttataag aatagctgat
    10321 gtttattaag ttaatattaa gcagtgaaat ttagtctata agatatttgg caaaaaaaag
    10381 cccggtgcaa tatacgccgg gcctcaattt tattgttggt taaatcagat taatccagcc
    10441 attcagtatg gaacacacct tctttatcaa tgcgcttata agtatgcgcc ccgaaatagt
    10501 cacgctgtgc ctggatcagg ttcgcaggca gaaccgcggc acggtagcta tcgtaatagg
    10561 caaccgcagc ggcgaaggtc ggaaccggga taccgttctg tactgcataa gcgacgacat
    10621 cgcgcagcgc ctgctggtag tcatcggcaa tttgcttgaa gtacggggcc agcagcaggt
    10681 tagcgatctg cggattttcg gcataagcat cggtgatttt ctgcaggaac tgcgcacgga
    10741 tgatgcagcc agcacggaaa atcttcgcga tttcgccgta gttcagatcc caattgtatt
    10801 cttcagacgc agcacgcagc tgagagaagc cctgagcgta agaaacgatt ttgcccagat
    10861 acagcgcact gcgaactttt tcgatgaact cagccttgtc gcctgctgac tgagcttgcg
    10921 gaccagagag aactttagat gcggcaacgc gctgatcttt cagagaagag atataacgtg
    10981 caaacacaga ctcggtaatc agcgacagcg gttcgccgag atccagcgcg ctctggctgg
    11041 tccatttacc ggtgcctttg tttgctgctt catccagaat cacatcaacc aggtagttac
    11101 cgtcttcatc ttttttggtg aagatatctt tagtgatgtc gatcaggtag ctgctaagtt
    11161 caccgttatt ccactcggtg aaagtctggg ccagttcttc attagataga ttcaggccac
    11221 ctttgagcag agaatacgct tcagcaatga gctgcatatc tccgtattca ataccattgt
    11281 gaaccatctt cacataatgg cctgcgccgt cggcaccaat ataagtaacg catggctcgc
    11341 cgtcttctgc cacggcagcg attttagtca ggataggcgc aaccagctca taagcctctt
    11401 tctgcccgcc aggcataata gaaggacctt tcagcgcgcc ctcttcaccg ccggaaacac
    11461 cggtaccgat gaagttaaag ccctctgctg aaagctcacg attacgacga atggtgtcct
    11521 ggaagaaggt attaccacca tcaatgatga tgtcaccttt atcgagatat ggtttcaggg
    11581 aatcaatcgc agcatccgtg cctgcacctg ctttcaccat taacaggatg cgacgaggcg
    11641 tttccagaga ttcaacaaac tctttcaccg tatagtaagg agccagtttc ttgcctggat
    11701 tttcagcaat cacttcttcc gtcttttcgc gggagcggtt gaaaatagag acggtataac
    11761 cacgactttc gatgttgagc gcaaggttgc gccccatcac tgccataccg actacgccga
    11821 tctgttgctt tgacattgtt tactcctgtc ttctatatta ttaaccgtct attaaccgtt
    11881 atattattaa ccgtcgtgat tttcatcttc taatcaagga atacatatgg ttacagcatg
    11941 cttcttactt tatagcgata agtatgccat tgggtgtatc cataagaact ttgaattttg
    12001 gatacttttc tggattaaaa ttagcatagc ttcg
//
LOCUS       DAZXUL010000123        12002 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZXUL010000123 DAZXUL010000000
VERSION     DAZXUL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12002
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.123"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..2251)
                     /locus_tag="ACH3L1_003418"
     CDS             complement(<1..2251)
                     /locus_tag="ACH3L1_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HFT4078036.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTEGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="ACH3L1_003419"
     CDS             complement(2275..2898)
                     /locus_tag="ACH3L1_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFT4078037.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="ACH3L1_003420"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="ACH3L1_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACH3L1_003421"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACH3L1_003421"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFT4078038.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /gene="ubiG"
                     /locus_tag="ACH3L1_003422"
     CDS             7506..8228
                     /gene="ubiG"
                     /locus_tag="ACH3L1_003422"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFT4078039.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..11931)
                     /gene="yfaL"
                     /locus_tag="ACH3L1_003423"
                     /pseudo
     CDS             complement(8356..11931)
                     /gene="yfaL"
                     /locus_tag="ACH3L1_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2780 a         3325 c         2957 g         2940 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag ctatccagcc aggccccctg
     8881 cttctgatta ccgtgctgat accagcttga ggtcagccca acggcataac cgtggttctg
     8941 gttatcggcg cgagttccgg tcatattcga gcggctgtcg ccctgattat cactgtagcc
     9001 accaaccgcc ccaagcatcc actcgccatc cgtgccccag cgcccgctaa acaagccgcc
     9061 gctaagctgc accgtagaag tgtcttcctg ctgagccagt tgccctgccg ccgtgtaatg
     9121 ataacgtccg ccgataacac gtaaattcag cgtctgacca tcgccaccag cgtgatcgcg
     9181 tcgctccatc ataaacgcct gatttgccgc ccgcaggtta ttgagataac cgccaacttt
     9241 ggcattcagc accggctggt aagcaggcgt aggggccggg tcgggggttg ggtcaggatc
     9301 cggcgtggga tcaggatcgg gagtcgggtc tggctcaggt ggcgatggcg gcgttacttc
     9361 ttgcgatcgc agataccagt cgttgttatc ttccactagc gtgtagtcat acgctcccat
     9421 gttgacgtag ccactgccag ccagactgaa ctgtgcattg ttttgaaact gcgtgggatc
     9481 agctgcgaaa tcaaccactt taatgcctgt cgatgtcggc tcaccaatcc ctgtaatgga
     9541 gttaaccacc acagttgtgt tgccagcagt attaccgttc atcaccaatt gatcgcttac
     9601 cgagtcatcg ccgtttaatt cgctgtcgaa tagtagcgta ccgccgccgg tataatcacc
     9661 gtttaccgtc agcgtatcgc cagcgacgcc attttgcagg ctaaccgcac cggacgtatt
     9721 ggtcagatca ccattaaccg tcacgtcagc aagataggtc aggctgtcag tttcaaggtt
     9781 ggtattaagc tcgcctgtca acgtcacgcc atcggtagca tttaccagcg tcccatcgcc
     9841 gttaaatagt gaggcattaa gggcgacgga agtatcctgc ccggtcagac gcaaaaccgt
     9901 accatcgctg atgtcgatag tgccgctgga agtagcatca attgactgaa tatcctgatc
     9961 cgcgccagta acgaacgttg ccccatcacc aacccacagt gacgaagcat aaggaaagat
    10021 atccgcaaca tcgcctttca gggtgccttc ttcgacacgt accgccgact gcgttgtacc
    10081 gctggcggtc agctccagcg tacccgcccc cgttttagta aatgtgctac cctcatcctg
    10141 atgctgcccg ctgctgtcag ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac
    10201 tgccacttca ccgtcggcgc gcatttcaat atcacgccca tgctcagtcg tcgtagagcg
    10261 agtatcgaca ttggcagtaa tctccatcac gctttcttgt tacttatcgg taaagataac
    10321 ctggcggtta tagtgggtat ctccaagctg cgagttgtcg ctcaccatca gcgtaccgga
    10381 ggcgatttgt gttgtgccca aataactatt attgtttgcc agacttacct gcccgtcgct
    10441 cgcatcgacc accacgtaga cgccatcttt cccgtcgccg ccgatatgca aattgtcgcc
    10501 acctgcaaga tctaccacat cctgactgcc gataaccgtt ccgctgctgc cactgacttc
    10561 aaggccatca ttgtatgatg tgccgctctg ccaagtggag aaatcagaga gatcgagcac
    10621 accgccgttt aacacgatcg actgcggatc gtcctggaga gcggtaagat ctgccgcgtc
    10681 gccttccagc gaaagcaccg caccatcatc aacgactata tcgccggtta gcgccatcga
    10741 ctgcgcccct gccagcacgt agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc
    10801 gatgatgcca gcaaaactgc cctgattaac agtaacgttg ccaccagcat cgatatttaa
    10861 agtgccattc tgaaagcccg tcaatgcgtg cacaaaagtt tgttgggtcg agccaacgtt
    10921 tagctcagcc tgattctgat actgatcaat actccctatc gtcagaccgt agcagtcttg
    10981 cggatcgtcc tggcaatgcg tatcgccgac attcatcagg gagttgctgc gacccagggt
    11041 aacttcaccg ttttcaatct gcatctcacc agtaaagtca ttgttatctg cattaagtac
    11101 cagatcgccg gaacctgttt tggtgattaa cccggtacca gcaatagagt caacagctcc
    11161 gtcattctct gtattgccga taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc
    11221 acttaagccg agatacataa agccacccgc cgcagtggag ggaccatctc catagcctgc
    11281 tgcgctattg ttctcatcga ctaacacgcc gccgttctgg ctgtagctgt catcaacaga
    11341 aatatcaata agatagggag ccgtcgcgct attggtatat atcgccccgc cataaccttc
    11401 ggctgtgtta tttgtaaagg cggtgttatt tattatcgta taacctgaag gatgcttgct
    11461 gtcgctatta ttatcggtaa catcgattgc cccgccatcg ccatcactgt aacttgttga
    11521 tgtatatgcc tggttattgt taaaaacatc atcacttaaa tagacgtcat tattaatggt
    11581 ataaattgcg ccacctttgc catcattagc gatattattg atggcgttag tgacacgtaa
    11641 atcgacggca ccagtatcat tagtaccaga agaatagatt gcgccaccat agccgcctgc
    11701 gacgttaccg gaaaaaataa catcagtaag attcagcgtt gagttttctt tagcaaatat
    11761 ggccccgccg ttattatatt ctcctgtaac ggtgttattg gcaaacagag tcattccagt
    11821 ttcattttct ggtaatagtg aaaattccgc tccttgttgc aaaaatacgg ccccaccgct
    11881 ggcattattg gtcatatccg aaaaaaccag ccattgccca tcggcgatag actggccccc
    11941 tgaatctcca gacaaccagt atcacttatt taagtgatag tcttaatact agtttttaga
    12001 ct
//
LOCUS       DAZXUL010000124        11824 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZXUL010000124 DAZXUL010000000
VERSION     DAZXUL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11824
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.124"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..407
                     /locus_tag="ACH3L1_003424"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACH3L1_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..4690
                     /gene="pic"
                     /locus_tag="ACH3L1_003425"
     CDS             572..4690
                     /gene="pic"
                     /locus_tag="ACH3L1_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFT4078040.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4841..5083)
                     /locus_tag="ACH3L1_003426"
                     /pseudo
     CDS             complement(4841..5083)
                     /locus_tag="ACH3L1_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5122..6072)
                     /locus_tag="ACH3L1_003427"
                     /pseudo
     CDS             complement(5122..6072)
                     /locus_tag="ACH3L1_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6123..6698)
                     /locus_tag="ACH3L1_003428"
                     /pseudo
     CDS             complement(6123..6698)
                     /locus_tag="ACH3L1_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6702..7102
                     /locus_tag="ACH3L1_003429"
                     /pseudo
     CDS             6702..7102
                     /locus_tag="ACH3L1_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7505..8086)
                     /locus_tag="ACH3L1_003430"
     CDS             complement(7505..8086)
                     /locus_tag="ACH3L1_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078041.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(8133..>11824)
                     /gene="sigA"
                     /locus_tag="ACH3L1_003431"
     CDS             complement(8133..>11824)
                     /gene="sigA"
                     /locus_tag="ACH3L1_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFT4078042.1"
                     /translation="LDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELP
                     VPDFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVH
                     RLNKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQY
                     LFGQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVF
                     HGVTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLS
                     VSGGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVA
                     GDNLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALS
                     ESGNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYH
                     GNVSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKE
                     GNNNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKL
                     NLENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGD
                     SAGESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKA
                     IELAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASI
                     LFGVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSAL
                     QSLKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLT
                     GSNNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQ
                     EHTTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWA
                     RIMSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTK
                     SVGAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRY
                     HVTEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGK
                     DWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFG
                     LEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         2994 a         2788 c         2857 g         3185 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgtccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgggta gtggtacgca gtacactaag gacaaggacg gaaatttagt
     1201 taaggttgcc ggtggatatg cttttaaaac aggaggaacc acaggagttc ctctgatatc
     1261 tgatgcaaca atagtctcta atcccgggca aacttataat cctgtaaacg gccctttacc
     1321 tgactatgga gcccctgggg atagtggttc tcctttgttt gcttatgata aacaacaaaa
     1381 aaaatgggtt attgttgctg tattaagagc atatgcaggt attaatggtg ctacgaactg
     1441 gtggaatgtc ataccaacag attatctgaa ccaggttatg caggacgatt tcgatgcccc
     1501 cgtagacttt gtttccggac tgggccccct gaactggaca tacgacaaaa catcaggcac
     1561 aggtaccctg agccagggca gtaaaaactg gaccatgcac gggcagaaag acaatgacct
     1621 caatgccggt aaaaatctgg tattcagcgg gcagaatggt gcaattatcc tgaaagacag
     1681 tgtgactcag ggtgccggtt atctcgaatt taaagacagt tacaccgtat ctgctgaatc
     1741 cggaaaaaca tggacgggtg ccggcattat tactgacaag gggacgaatg taacctggaa
     1801 ggtcaacggc gttgccggtg acaacttgca taagctgggg gaaggaaccc tgaccataaa
     1861 cggaacaggt gtaaacccgg gaggactgaa aacgggagac ggtatcgttg tacttaacca
     1921 gcaggcagac actgcaggta atatccaggc cttcagttca gtgaacctcg ccagcggacg
     1981 tccgaccgtg gtgctcgggg atgcccgtca ggtcaatccg gataacattt catggggata
     2041 ccggggaggt aagcttgacc ttaatggtaa tgccgttacc ttcacccgac tgcaggctgc
     2101 tgattacggg gcggtgatta caaataatgc acagcaaaaa tcccagcttt tactggatct
     2161 taaggctcag gatacaaatg tcagtgaacc gacgattgga aatatatccc cctttggtgg
     2221 taccggcact ccaggaaacc tgtacagcat gatactcaac agccagaccc gcttctatat
     2281 tctgaaatct gccagctatg gtaacactct gtgggggaac agcctgaatg atccggctca
     2341 gtgggagttt gttggcatga acaaaaacaa agcagttcag acagtaaaag ataggatcct
     2401 ggccgggcgg gcaaaacaac ccgttatctt tcatggtcag ctgaccggga atatggatgt
     2461 cgccattcca caggtgccgg ggggaagaaa ggtaatcttt gatggtagcg tgaacctgcc
     2521 ggaaggtacc ctgagtcagg acagtggcac cctgatattc cagggacatc cggttatcca
     2581 tgcctccatc agtggcagtg caccggtcag cctgaaccag aaagactggg aaaaccgtca
     2641 gtttacaatg aaaacactgt cgctgaaaga cgctgacttc catctttcac gtaacgcctc
     2701 gctgaacagt gacattaagt cggataacag ccatatcaca ctgggaagtg acagggcatt
     2761 tgtggataaa aatgacggaa caggaaatta tgtcattccg gaggaaggta cctctgtccc
     2821 ggacaccgtg aatgacagga gccagtatga agggaatatt acgctgaacc ataactcagc
     2881 cctggatatc ggcagcaggt tcaccggggg gattgacgct tatgacagtg ccgtcagcat
     2941 cacctctccg gacgtcctgt tgacagcccc gggtgctttt gccggcagtt cactgacagt
     3001 gcatgatggc ggtcatctta cagcactgaa cggtcttttc agcgacgggc atattcaggc
     3061 cggtaagaac ggcaaaatca ccctgagcgg tacaccggtt aaagatacgg ctaatcagta
     3121 tgcccctgct gtatatctga cggacggata tgacctgacc ggcgataacg cagcactgga
     3181 aattacccgt ggagcacatg cttccggtga tattcatgcc tctgcggcat caacagttac
     3241 catcgggtct gacacgccgg cagaactggc ttctgcggaa acggctgcat cggcgtttgc
     3301 cggcagtctt cttgagggct ataacgcagc attcaatggt gccataaccg gtggcagggc
     3361 tgatgtcagt atgcataatg cactgtggac tctgggtggg gactctgcca tccacagtct
     3421 taccgtcaga aacagccgta ttagttctga aggagaccgt acattccgta ccctgacggt
     3481 gaataaactg gatgcaacag gcagtgattt tgttttgcgt acggacctga aaaatgccga
     3541 taaaattaat gtgactgaaa aagccactgg ttcagataac agcctgaacg tcagctttat
     3601 gaataatcct gctcagggac aggccctgaa tattcctctg gtcacggcac cggcgggaac
     3661 ttcagcagag atgtttaagg ccggcacccg ggtgacaggt ttcagtcggg tgaccccaac
     3721 cctgcatgtt gataccagtg gtggcaatac gaagtggata ctggatggtt ttaaagcgga
     3781 ggctgataaa gccgctgccg cgaaggctga cagtttcatg aatgccgggt ataaaaactt
     3841 catgacggaa gttaacaatc tgaacaaacg tatgggtgac ctgcgtgaca caaacggtga
     3901 tgccggtgcc tgggcgcgca tcatgagtgg tgccggttct gcagacggtg gttacagtga
     3961 taattacacc catgttcagg tcggctttga caaaaaacat gaactggacg gtgtggacct
     4021 gtttaccggt gtcacgatga cctataccga cagcagtgca gacagccatg cattcagcgg
     4081 aaagacgaaa tcggtggggg gcggtctgta tgcttcagca ttgtttgagt ccggtgccta
     4141 tatcgatttg attggtaaat atattcacca tgacaatgat tacacaggta actttgctag
     4201 cctgggaacg aaacactaca acacccattc ctggtatgcc ggtgctgaaa cgggttaccg
     4261 ctatcacctg acagaggaca cgttcattga gccgcaggct gaactggttt acggcgccgt
     4321 gtccgggaaa acattccgct ggaaagacgg tgatatggac ctgagcatga agaacaggga
     4381 cttcagtccg ctggttggaa gaacaggggt tgaactgggc aagaccttca gtggtaagga
     4441 ctggagtgtg acggcccgtg ccggaaccag ctggcagttt gacctgctga ataatggaga
     4501 gaccgtactg cgtgatgcgt ccggggagaa acggataaaa ggagagaagg acagccggat
     4561 gctgtttaat gttggtatga atgcgcagat aaaggacaat atgcgctttg gtctggagtt
     4621 tgagaagtca gcctttggta aatataacgt ggataatgcg gtaaacgcga atttccggta
     4681 tatgttctga tgtgggggta aagggctgcc ggaaggcagc ctttctcaga gactcttaaa
     4741 atataaaact gaaggtcttc ggtaaggggg agtgtattag acgtcaacta gttttgccga
     4801 ctggcgcgag cctgtttaag attctgtgta aatgcctttc tcagaagtga ccgtccaggc
     4861 ggtcaccgaa ctcgataata aagcggctca ttgccattcg ccagtccttc aacggcatcg
     4921 tccatttctg ggacgcagac tggattgcca gccacaccac ttttttcacc gagtcgtctg
     4981 tcgggaacac tttacgcttt ttgatcgcat ggcggatcac gctgtttagc gactcgatgg
     5041 cattcgtcgt atagatcact ttgcggatgt ccgttggata agcggtaagc gtacagcgag
     5101 ggccgtattg acggggatgt gttattcagc tggcagtgct atgcgccacg gaagcagttc
     5161 gctgacccgg ttgaccggcc agtctgctat gacgccaagc acatggcgaa ggtagctttc
     5221 tggatccacg tcattcagtt tgcacgtccc gatcaggctg tacagtagcg cgccccgctc
     5281 accaccatgg tcagagccga agaacaggaa gtttttacga cccagactga ccgcccgcag
     5341 ggcattttca gcgatgttgt tgtcgatttc cacccagcca ttactgcagt acacgttcag
     5401 ggccttccac tgtttcagta ggtatgcgaa cgcttttgcc gtatccgagt gacgcgacag
     5461 tgttttcatc tgttgctgta tccggtcata cagtgactgc atcagtggcg cggctcttgc
     5521 ttttcttgcc gccagacgct gttctgctga acatccccgg acttctgcct cgatggtata
     5581 cagttcaccg atacgctgca gggcttccgt ggtgatgtcg gttggcactc ttgcatgcac
     5641 atcgtggatt tttctccggg catgggccat acacgcagct tccgttattc tgccggattc
     5701 gtataacgcc cggtaaccac cgtaagcatc tgcctgaagc acaccgctgt aaccggctag
     5761 gtgattctgt ggatggatac ctttccggtc cgggctgtac gcgaaccaga ccgccggggg
     5821 catctgtgaa ccggcgttac ggtcatcacg gacatagacc cacagccggg cagttcttgt
     5881 tttaccactg cccggctcct ggaccggaac ggggatatca tccgcatgaa ctttaccggg
     5941 catcagtaca tactggcgca ggacgtcata cagcggctcc agcagttcag caacggcacc
     6001 ggtccagcga cccagtgtgg cacggctcag ctccacgccc tgacgacggt atatttctga
     6061 ctggcggtat aactgtcaac gacggatgaa aagtggctct tccgcaacct gggtggagat
     6121 tttcatgcca gtggactcat taccctctgc ctgagtagtt caaatcctgc tcgaccatac
     6181 atctggcgtt tcagcatctt cttcaggcga tttacatgac cttcgacaac accattactc
     6241 cagcgactac tgattgcctc acatatagct gcagcatcag cttccatccc cgccgccacg
     6301 cgccgaaatt ctgctgaacc gctttcgtgg actcgagtga accaactgct tagctgtgat
     6361 ttattttggg ttttcagaat acggtagaac tcgagtgcca gttgctgcgc tattttcagc
     6421 tcaggttctt tttcacacat cagactaata aatcgggaag cataattttc ttcatccctg
     6481 gttattctcc agggcatcaa ccagcggctt acccgggaga ctgaaggaag tctgacggca
     6541 gtagtaaccg gtgggttcca gcctttacgc catttggcaa ccgcatccct gacgatggtt
     6601 tcactgcctg taaatccccg ggccaccatt tcccgccata tccggctggc gttataatta
     6661 ccactttctc gttgctcttt taaccactcc ctccatggtg tcagcgaaaa acgaaaccgc
     6721 tgttgaaatc cctggaaagc tggttgcgga aaagatgaag acccagtcgc gacactcaga
     6781 gttggcgaag gcgttcgcgt acgcacttaa ccagtggccg gcactgacgt actatgcgaa
     6841 cgatggctgg gtggaaatcg acaacaacat cgctgaaaat gccttgcggg cggtcagtct
     6901 gggtcgtaaa aacttcctgt tcttcggctc tgaccatggt ggtgagcggg gagcgctact
     6961 gtacagcctg atcgggacgt gcaaactgaa tgacgtggat ccagaaagct accttcgcca
     7021 tgtgcttggc gtcatagcag actggccggt caaccgggtc agcgaactgc ttccgtggcg
     7081 catagcactg ccagctgaat aacacatccc cgtcaatacg gccctcgctg tacgcttact
     7141 catcacctgc tattaatccc cgagtcagta cttcgcggcc tgacgcgatc accctactca
     7201 gtacttcaga gcccgcctcc ggtacctgcc tggcgatcgc ccctgtgcaa caaaataaat
     7261 caaccgttat caaaccacga tcgttcgtat gcgccgccga tgacgcgcgc ctgaaaaacg
     7321 cgtcaatatg agttccccgg aagcttacga taatacgctt atgaacatta caacacgctc
     7381 atgcggaacc tggatggggg aaggaacggt gaagctgtta gcgaatgacc ggcgtttata
     7441 caaacgccgg tcatgatgaa tgatatttca gaatatacct cgggctaaaa taccgggaca
     7501 gagatcaggc gtgattaatc tgcagcattt ttaataacag aagtactgag cttactttac
     7561 ctttgatgat gtaatagtta tttccgtcgg tactcatttt tccctgaaat aatgtcacgg
     7621 atgtttcttc ctgctgatcc tgcaggcgca gtcgtaaggt catattttta atgagctgag
     7681 atccgggtgg caaggggtat ttgctgtcag atactgggcg actgagtctg atgtggcgcg
     7741 tatacagccc gttttcgtac agggtggcct ctgttatggt gtcagtgacc agcatgtggg
     7801 agacaatgct gtattctttt ttaaagcacc caccagaaac ggtgattgta atgataatgg
     7861 tcttctcgat cgagatgtaa atacccgttt cgttatactg tccgtatcgg aaccatgaga
     7921 actcagttgc attccagcca tgctccagaa tatgtgcatt tttttgcctg atagtttctt
     7981 tttttattcg ctcatgttta aggcataaca ggcagataaa taatgtcaga aaaagaaagc
     8041 taataatgag agcaagagcg atatagtctg tcacagggta atacatattc tctccggtag
     8101 aagaagggcc gcaaacgcgg cccgggctgt tatcagaaag agtaacggaa gttggcgttg
     8161 atcgcgttat ccacgttgta tttaccaaat gccgatttct caaactcaag accgaagcga
     8221 agattatcgc gaatttcggc gttgagacca acattcatga gaatacgacc gtctttttca
     8281 cctttgatac gtttctcacc ggacgcatca cgcagtacgg tttcaccgtt ggcaaacagg
     8341 tcaaactggt agccaaggcc ggcgcgggct gtgactttcc agtccttacc ggagaaggat
     8401 ttacccacat caacaccggt acgcccaatc agcggattaa agtccttatc cttcatggtc
     8461 acgctcattc cgcgatcctg ccagtcgaac cgtttacctg atacggcccc ataaaccagt
     8521 tctgcctgcg gctcaatcca ggagtcttct gttacatgat agcggtagcc tgcttcagca
     8581 cccacataca aggaatgaga actgtagtct tttgttccga gtccagcaaa ggtcgccgag
     8641 tactcattat catggtgaac atacttacta atcaggtcga tataggcacc agagtcaaat
     8701 atagcggaag catacagacc tgccccgacg gacttcgtct tgccactgaa tgcattactg
     8761 ctggcatgac tgtcggtata cgtcatagtc agaccagtga aaaggtcaag tccatccagc
     8821 tcatgttttt tatccacacc aatctgcaca tgggtgtagt tgtcactgta tccaccagaa
     8881 gctgaacctg ctccgctcat gatgcgggcc catgcaccgg cctcgccatt ggtgtcacgc
     8941 agatccccca ttcgtttatt caggttgttg acttctgtca ggaaggcttt gtaattaccg
     9001 gacatatacg attttgctgc accggaagat gctgtatttt cagccacctt atatccggtc
     9061 agggtccacg tggtatgttc ctgatcctct ttcgtgctga tcacaggaga tatgttgctg
     9121 aaacctatgg cctgagtact ggctttaaat atctctttat tgcttccttt tggggctgtt
     9181 atcaggtcaa cgtttaatcc tgagttatta tttcccactt tctgtatatc gaccgacaga
     9241 ctgttattgc ttccggttaa ctgattttta acaatgagtt tatccgattg atttacagac
     9301 tggctcagat cagcccgcat aatcacagca gtattagtgg catccagtgt gtttactgtc
     9361 acagtatgga aacccgaatt ctctatgtta ctaaacaaca aaagactgtc gcgggttttt
     9421 aatgattgca gggctgactg tccgtttact ctccataagg tgttatacat gctggctgag
     9481 gctgcaggag catcaatacc tccggtataa gaagtatcaa agccacccag caaacctgta
     9541 gccagcggtg tgtgtgtggt tttgtccagg ttatcttctt tatactggtc aacaccaaac
     9601 agaatagatg cttttttgtc tgattttata ttaccattga cccaggcata attttgagcc
     9661 tttaacgtgg caccatcttc tgtcatatta tatccatctg acatatagat tgccgggctg
     9721 tatttattat tttctgtagg ggtgcctgtt aatgtcaggg ttccggccag ttcaatagct
     9781 ttatctacat acatttttcc ctgggcggta acagtagctc ctttgttgat aatcaggtct
     9841 gtattaatta aggataatgc accagtcata gtaacagttg atttatcacc aacagttatt
     9901 ttgctgtttt gagcactcag actgccgttg aaactacttt cgcctgctga atccccggag
     9961 cgaagctgtt gtctgaatcc aaaaccttcg ccagtaatgt ttcttcctga gaacatgtca
    10021 atgtatgcag ttttatttcc cagaacaatt tgagagtttt tagcctctat gtgtcctttt
    10081 acattagcat cccggcctat actcagggtt gcgttttcta aatttaattt ccggaaatta
    10141 aatttacgac tttcccagtc gggctgggaa aaagaggcta tctgattgtt ggacttatat
    10201 tccgtattgt aacgtttatt tacagtgctt tcctggtcct ttatggcagc agaatagtct
    10261 ttttgacata aaaaaggaat tggacagttg ttattgcctt ctttaaatat ggcatgttct
    10321 gtcgcatgtc cttgtaatgt taactgtgca ttccggacag agatatcgtt tttgatatcg
    10381 actgagccat caaagatcag attggcatta ttgttatgtt gctgggtagt attgataata
    10441 tgatttatgc ttatattacc tgatacattc ccatggtaca tatatgcatc agtattagta
    10501 agtgataatg ttgattgctt cacgtttgag ttcgttacgg ttgttcccgc atctgttgct
    10561 gcgattttct gaaatgactg gtcatagccg tttagatcca gtgttccgcc atttctggta
    10621 aaatagattt cgccattacc gctttcgctt aaagcgtctt tggcatttat ttttaccgta
    10681 cctttacctc cggccatgta aattttattg aaggcttttt cagcactaag gatgacagtc
    10741 ccattaccta ttttaaggtt atttccctgt gctattttta catccagagt accactacct
    10801 attttatgca gattatctcc ggcaacgccc ttaacattcc attcaacggt ggtattatta
    10861 tcaatatcta tcccggcacc tttataagac ttatcttttc cgtacacttt atatgtctgg
    10921 ttcttatcga atataagccc gccaatgcca agatcggtat cctgcttgag cgttaatacc
    10981 cctcctcctg agacagacag gtccttatct tttataatcg atgataactc ttgtttttta
    11041 ccatccagct gcatagtgtt tgtgaaattc ttttttaaat tgtttaccag aatattatta
    11101 aataacgagt agtttgtcca ggctatattg gtactactga taaaaccaac tgtactggta
    11161 acaccatgga aaacccattt ttgttgttct ttatcatata gataagaacc agaaccacta
    11221 tcgccaaaaa ttggtgagtt tttaaaaggg gttctgttgg tataaatgta actcttgttt
    11281 ttccagtcca gattaaaaag acttgcgctt aacaagtcgg gattatagtt ttgtccaaac
    11341 agatattgtt tgccgtttaa tatcgttgag gttgttccgg cacctgctct gaagccaatt
    11401 atttgttcct taccgttgta gtttacgcca tatcttttca tcgcatcttc gggagaaagt
    11461 gagaagtcgg cactctccgt aacccctgtt gtttccacga cgaatttatt cagacgatga
    11521 accgaaaagt cgccagatga tactctgtta cttgctttat atgctgtctg atcccatgac
    11581 tgtgttgtta ttgcatgatg ctgtgtaccg ttatgagtcg cagtaacact gtacgcacct
    11641 ccaattgatg ttgttgcacc tttgttggaa acagcagaaa aatcaggtac aggtagttct
    11701 ggaaaatgaa atatctttcc atctttcatc attaattgaa catttgttgc acccggtctg
    11761 aactctcctc tgttttgagc aaggtcaaga taatcgcggg tccatatatt atttatatcc
    11821 agca
//
LOCUS       DAZXUL010000125        11682 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZXUL010000125 DAZXUL010000000
VERSION     DAZXUL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.125"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..346
                     /locus_tag="ACH3L1_003432"
     CDS             <1..346
                     /locus_tag="ACH3L1_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFT4078043.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
     gene            complement(463..738)
                     /gene="yjfN"
                     /locus_tag="ACH3L1_003433"
     CDS             complement(463..738)
                     /gene="yjfN"
                     /locus_tag="ACH3L1_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFT4078044.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="ACH3L1_003434"
     CDS             complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="ACH3L1_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFT4078045.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1398..2147
                     /gene="yjfP"
                     /locus_tag="ACH3L1_003435"
     CDS             1398..2147
                     /gene="yjfP"
                     /locus_tag="ACH3L1_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFT4078046.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="ACH3L1_003436"
     CDS             complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="ACH3L1_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFT4078047.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="ACH3L1_003437"
     CDS             complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="ACH3L1_003437"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFT4078048.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4426..5823
                     /gene="ulaA"
                     /locus_tag="ACH3L1_003438"
     CDS             4426..5823
                     /gene="ulaA"
                     /locus_tag="ACH3L1_003438"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFT4078049.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5839..6144
                     /gene="ulaB"
                     /locus_tag="ACH3L1_003439"
     CDS             5839..6144
                     /gene="ulaB"
                     /locus_tag="ACH3L1_003439"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFT4078050.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6154..6618
                     /gene="ulaC"
                     /locus_tag="ACH3L1_003440"
     CDS             6154..6618
                     /gene="ulaC"
                     /locus_tag="ACH3L1_003440"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFT4078051.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6632..7282
                     /gene="ulaD"
                     /locus_tag="ACH3L1_003441"
     CDS             6632..7282
                     /gene="ulaD"
                     /locus_tag="ACH3L1_003441"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFT4078052.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7292..8146
                     /gene="ulaE"
                     /locus_tag="ACH3L1_003442"
     CDS             7292..8146
                     /gene="ulaE"
                     /locus_tag="ACH3L1_003442"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFT4078053.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8146..8832
                     /gene="ulaF"
                     /locus_tag="ACH3L1_003443"
     CDS             8146..8832
                     /gene="ulaF"
                     /locus_tag="ACH3L1_003443"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFT4078054.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8961..9236)
                     /gene="yjfY"
                     /locus_tag="ACH3L1_003444"
     CDS             complement(8961..9236)
                     /gene="yjfY"
                     /locus_tag="ACH3L1_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFT4078055.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9563..9958
                     /gene="rpsF"
                     /locus_tag="ACH3L1_003445"
     CDS             9563..9958
                     /gene="rpsF"
                     /locus_tag="ACH3L1_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFT4078056.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9965..10279
                     /gene="priB"
                     /locus_tag="ACH3L1_003446"
     CDS             9965..10279
                     /gene="priB"
                     /locus_tag="ACH3L1_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFT4078057.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10284..10511
                     /gene="rpsR"
                     /locus_tag="ACH3L1_003447"
     CDS             10284..10511
                     /gene="rpsR"
                     /locus_tag="ACH3L1_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFT4078058.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10553..11002
                     /gene="rplI"
                     /locus_tag="ACH3L1_003448"
     CDS             10553..11002
                     /gene="rplI"
                     /locus_tag="ACH3L1_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFT4078059.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11073..11636)
                     /locus_tag="ACH3L1_003449"
                     /pseudo
     CDS             complement(11073..11636)
                     /locus_tag="ACH3L1_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2767 a         2871 c         3087 g         2957 t
ORIGIN      
        1 cggcaatatt atgtgcctgg atgtgttgcg cgttctcaat aagcaagcgg gcgtatacga
       61 cttattgtcg gaagcatttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt
      121 tcgtcgttta cagcagcagc tgcgtaagcc agctgaagaa ctggggcgag agattactca
      181 tcagctattc ctgctgggct gcggtgcgca aatgttgaaa tatgcttctc cgccaatggc
      241 gcaggcgtgg tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat
      301 ccagaatgat ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga
      361 tgcgacgctg gtttcgatta actaaatgaa atatgtgaaa attgtaggcc ggacaaggcg
      421 ctcgcgccgc atccggcatt gttcatttgc tgtagatgac actcatgcat acagtatcgc
      481 ctgtacgcgc cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga
      541 cgcaccttgt tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga
      601 aatattgcta actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc
      661 gaattctgca gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga
      721 ggcaagtaat tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt
      781 ccatcgcggt aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc
      841 atagggacgc aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca
      901 aaatggcttg tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt
      961 aataatcagc ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag
     1021 aaccccgaac catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc
     1081 gaatctcttg cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc
     1141 tacaggcact taacatcaac accaataata aactggcaaa ccggtaaata acgctattac
     1201 gtttcctgct aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa
     1261 ttgttacctg gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa
     1321 cattatgaac taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag
     1381 acaaaaagga gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc
     1441 ttcatgctta tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg
     1501 gctttacttc atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt
     1561 tgcgggtgat catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc
     1621 ggcggttaaa tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac
     1681 gtgcggcaat agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt
     1741 cgatgggcgc gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg
     1801 ccagcatgat gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga
     1861 tacctgaaac ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt
     1921 gggaagcgac aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc
     1981 tcgacgatga cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga
     2041 cgggacggga taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc
     2101 ctgaggcgtt agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc
     2161 tgaccccttg cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca
     2221 gcatatcgat ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc
     2281 tatcaaccag taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg
     2341 tcagcatatc ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc
     2401 agtgcccggc atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt
     2461 tgttgtactg tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg
     2521 ccagcggtag ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca
     2581 gaaacgcggt ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg
     2641 ccgctttagc aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg
     2701 tccagcgcgg gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc
     2761 cgctttcgcc aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct
     2821 caacgacttt ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat
     2881 gtctttgtgc ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg
     2941 aaatagccag gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac
     3001 gacagattac aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa
     3061 accgcgcgga tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc
     3121 aacctgccag ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag
     3181 cacgcggatc tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat
     3241 aaccactttc gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc
     3301 ggtaataccg cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt
     3361 accgtgcttc gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc
     3421 aggggttttg aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc
     3481 cgctttttga tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg
     3541 aatttcaatg tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt
     3601 cggtacgccc cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc
     3661 atcagcacag ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc
     3721 gtgagtcgcc agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt
     3781 ggtgcgtagg tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg
     3841 tttcattaac gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac
     3901 gttggtgcca ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa
     3961 tgtgccaggg gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg
     4021 gaaagtgctc aggatccagg attctcgggt gatacttttc actttactca tcgccattta
     4081 ccttcatgat agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat
     4141 tccttcagga aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt
     4201 gagtggggaa agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata
     4261 attgtttcgt tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca
     4321 tttaatcata aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt
     4381 ccgggtgtgc ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac
     4441 aacatcttta ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt
     4501 gtgacctgtc tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg
     4561 attaaaacca taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc
     4621 ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat
     4681 acttacgcgt cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt
     4741 tacgcggtgt tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc
     4801 ggtattcgta ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc
     4861 gttacgttgt ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg
     4921 gtttcgctct actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg
     4981 gacggctgcg gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa
     5041 gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg
     5101 ctgaatattt tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt
     5161 gccattctgc tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg
     5221 acggtctata tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag
     5281 ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg
     5341 atccctggtg cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc
     5401 gtggtctggg gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc
     5461 gcctgcggtt cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc
     5521 accatcggcg tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg
     5581 gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt
     5641 gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc
     5701 tccatcggta tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc
     5761 gctggccgcg cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct
     5821 taataaggag ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc
     5881 agttccatga tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac
     5941 acggtaaata gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc
     6001 atcgcttcta cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc
     6061 gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg
     6121 catttcccgc aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag
     6181 aataaatcca tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc
     6241 gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     6301 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     6361 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     6421 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     6481 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     6541 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     6601 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     6661 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     6721 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     6781 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     6841 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     6901 ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca
     6961 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     7021 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     7081 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     7141 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     7201 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     7261 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     7321 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     7381 gttgggcttc gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct
     7441 tgactggagc cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg
     7501 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     7561 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     7621 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     7681 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     7741 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     7801 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     7861 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     7921 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     7981 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     8041 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgtgtgattg
     8101 ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa
     8161 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg
     8221 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     8281 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     8341 gaaggggagt atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac
     8401 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg
     8461 gggctggcga tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg
     8521 tgtacgcgtg gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa
     8581 gtgattatcg aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat
     8641 cagcacggac cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg
     8701 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat
     8761 atcgacagct acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac
     8821 gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt
     8881 ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
     8941 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
     9001 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct
     9061 tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg
     9121 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
     9181 catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat
     9241 acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta
     9301 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt
     9361 gacggaatgt gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt
     9421 atttctgctc cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca
     9481 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
     9541 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
     9601 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
     9661 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
     9721 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
     9781 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
     9841 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
     9901 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
     9961 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
    10021 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
    10081 agccggcttt caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa
    10141 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
    10201 atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
    10261 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
    10321 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
    10381 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
    10441 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
    10501 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
    10561 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
    10621 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    10681 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    10741 agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    10801 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    10861 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    10921 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    10981 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg
    11041 tcggcgtttt gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca
    11101 ttctcttgtg tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt
    11161 atgtctcggt cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt
    11221 gataaggaac gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat
    11281 gcagcaatga tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata
    11341 taaagatcga ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt
    11401 acaacgtatc ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca
    11461 acctcgtgta ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat
    11521 ttcagttctg cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaata
    11581 ttatctaatg tagcttcatg gttccatcca aaagaattat caccattcat tgttactgaa
    11641 tcgccacggg tttaacagac acctcagagt catttaagat gg
//
LOCUS       DAZXUL010000126        11655 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZXUL010000126 DAZXUL010000000
VERSION     DAZXUL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.126"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..794
                     /locus_tag="ACH3L1_003450"
     CDS             <1..794
                     /locus_tag="ACH3L1_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFT4078060.1"
                     /translation="SETKAAYRCFSNDKVSANKIMTPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="ACH3L1_003451"
     CDS             complement(744..1937)
                     /locus_tag="ACH3L1_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4078061.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="ACH3L1_003452"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="ACH3L1_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFT4078062.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="ACH3L1_003453"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="ACH3L1_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFT4078063.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="ACH3L1_003454"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="ACH3L1_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFT4078064.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="ACH3L1_003455"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="ACH3L1_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFT4078065.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="ACH3L1_003456"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="ACH3L1_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFT4078066.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="ACH3L1_003457"
     CDS             complement(10573..10716)
                     /locus_tag="ACH3L1_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078067.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..>11655
                     /locus_tag="ACH3L1_003458"
     CDS             10955..>11655
                     /locus_tag="ACH3L1_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFT4078068.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT         2956 a         2897 c         2992 g         2810 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tggga
//
LOCUS       DAZXUL010000127        11355 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZXUL010000127 DAZXUL010000000
VERSION     DAZXUL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.127"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            373..2013
                     /gene="trg"
                     /locus_tag="ACH3L1_003459"
     CDS             373..2013
                     /gene="trg"
                     /locus_tag="ACH3L1_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFT4078069.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2051..2974)
                     /locus_tag="ACH3L1_003460"
     CDS             complement(2051..2974)
                     /locus_tag="ACH3L1_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFT4078070.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3191..4534
                     /locus_tag="ACH3L1_003461"
     CDS             3191..4534
                     /locus_tag="ACH3L1_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFT4078071.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4759..6414
                     /gene="opgD"
                     /locus_tag="ACH3L1_003462"
                     /gene_synonym="mdoD"
     CDS             4759..6414
                     /gene="opgD"
                     /locus_tag="ACH3L1_003462"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFT4078072.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6554..6778
                     /locus_tag="ACH3L1_003463"
     CDS             6554..6778
                     /locus_tag="ACH3L1_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFT4078073.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6841..7377
                     /gene="rimL"
                     /locus_tag="ACH3L1_003464"
     CDS             6841..7377
                     /gene="rimL"
                     /locus_tag="ACH3L1_003464"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFT4078074.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7372..8352)
                     /gene="ydcK"
                     /locus_tag="ACH3L1_003465"
     CDS             complement(7372..8352)
                     /gene="ydcK"
                     /locus_tag="ACH3L1_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFT4078075.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8476..9468
                     /gene="tehA"
                     /locus_tag="ACH3L1_003466"
     CDS             8476..9468
                     /gene="tehA"
                     /locus_tag="ACH3L1_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFT4078076.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATSPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9465..10058
                     /gene="tehB"
                     /locus_tag="ACH3L1_003467"
     CDS             9465..10058
                     /gene="tehB"
                     /locus_tag="ACH3L1_003467"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFT4078077.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10361..11017
                     /locus_tag="ACH3L1_003468"
     CDS             10361..11017
                     /locus_tag="ACH3L1_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFT4078078.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(11035..11355)
                     /gene="tnpA"
                     /locus_tag="ACH3L1_003469"
                     /pseudo
     CDS             complement(11035..11355)
                     /gene="tnpA"
                     /locus_tag="ACH3L1_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2928 a         2656 c         2856 g         2915 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagttgat agaaatatcg
       61 gtcggggcct tcgtctttct gattcccggt tagcctgaaa acagaaagtc tcaggcaccc
      121 gcaggcatcc tatgaggttt ccttagggac gaaaataatc acttcacgaa attgcgtgct
      181 gttttccaga atttttcgtc attcgggtta gccagtttag ccattcgtta ctctcttcat
      241 tccaatagca ttaattttct ctgcaataat tgttgtaaaa atgtgacgca aagaggtttt
      301 tggtcataag taattaccgt caagtgccga tgactttcta tcaggagtaa acctggacga
      361 gagacaacgg taatgaatac aactccctca cagcgattag gttttttgca tcacatcagg
      421 ctggttccgt tatttgcctg cattcttggc ggtatcttag ttctattcgc attaagttct
      481 gctctggctg gctatttcct ctggcaggcc gatcgcgatc agcgtgatgt tactgcggag
      541 attgagattc ggaccgggtt agcgaacagt tcagattttt tgcgttcagc ccggatcaat
      601 atgattcagg ccggggctgc gagtcgtatt gcggaaatgg aagcaatgaa gcgaaatatt
      661 gcgcaagccg aatcggagat taaacagtcg cagcaaggtt atcgtgctta tcagaatcga
      721 ccggtgaaaa cacctgctga tgaagccttc gacactgaat taaatcaacg ctttcaggct
      781 tatatcacgg gtatgcaacc tatgttgaaa tatgccaaaa atggcatgtt tgaagcgatt
      841 atcaatcatg aaagtgagca gatccgaccg ctggataatg cttataccga tattttgaac
      901 aaagccgtta agatacgtag caccagagcc aaccaactgg cggaactggc ccatcagcgc
      961 acccgcctgg gtgggatgtt catgattggc gcgtttgtgc ttgccctggt catgacgctg
     1021 ataacattta tggtgctacg tcggatcgtc attcgtccac tgcaacatgc cgcacaacgg
     1081 attgaaaaaa tcgccagtgg cgatctgacg atgaatgatg aaccggcggg tcgtaatgaa
     1141 atcggtcgct taagtcgtca tttacagcaa atgcagcatt cactggggat gacagtaggg
     1201 actgttcgac agggcgcgga agagatttat cgtggcacca gcgaaatttc agctggcaat
     1261 gcggacctgt catctcgcac cgaagaacaa gcggcggcta tcgaacaaac tgccgccagc
     1321 atggagcaac tcactgcgac ggtgaaacag aatgcggata acgcgcatca tgccagcaaa
     1381 ctggcgcaag aggcttctat taaagccagc gatggcgggc agacggtttc cggtgtagta
     1441 aaaacgatgg gcgctatctc cacgagttcg aagaaaattt ctgagatcac cgccgtcatc
     1501 aacagtattg ctttccagac gaatattctg gcactgaatg ctgccgttga agccgcgcga
     1561 gcgggtgagc aagggcgtgg atttgccgtt gtcgccagcg aagtacggac actcgcaagc
     1621 cgcagcgctc aggcagcgaa agagattgaa ggcttgatca gtgaatcagt caggttaatt
     1681 gacctggggt cggatgaggt ggcaacggca gggaaaacca tgagcactat tgttgatgcc
     1741 gtcgcgagtg tcacacatat catgcaggaa atcgccgccg cctcggatga acaaagtaga
     1801 ggcataactc aggttagcca ggcgatttct gaaatggata aggtgacgca acagaatgct
     1861 tctctggtag aagaggcctc agcagcggcg gtgtcccttg aagaacaggc tgcacgatta
     1921 actgaggcgg tgaacgtatt ccgtctgaac aaacattctg tgtcggcaga acctcgcgga
     1981 gcgggtgaac cagttagttt cgctacggtg tgaaaatgtt caaggaggga tcgacagatc
     2041 ccttcacctt tcagaacggc attgattttc gaatagcgtt aatcatcaac tggcaaccag
     2101 aagagaacgt cgcatctacg cgggtcagta ttccaatcgg ttcgcctgca ccatgtcccg
     2161 gaacaggcag ggccaccagc gtggcatgac gcaggtcgtc ttttacagcg ccagaaggga
     2221 caaaccacac gtaatcgtat tcaacggtaa gttgacgaga tagcgaagta gacagcgttt
     2281 cgatacaacc cgaaggaatt ttacagccct ggctctgcac taatgcatct gaatgctggc
     2341 gtggcgcagt gccttctggt gatacaacga ccggccattc cagcacccgg cttagcgtta
     2401 cgttctcctg aagtagcggg tgattagggc ggacaaccag cttcaacgat tcaagaaaca
     2461 gcagttcgta attaagcccg gtcatcagtt caggatctga catccgacca atgccgatat
     2521 cgatttcccc ggtttttaaa cccgccagaa tcatagggtt actcattgtc gcaacttgca
     2581 aggtcgtctc tttttgttgc tgatgaaact gacctataac cgaaggtaat atccccagtg
     2641 ccgcagtagg tagtgcacca accctgacga catcattatt aagaccttct ttacgatgaa
     2701 gcgactgtcc ggcagtgttg atggcgtcaa gaactctgac tgcatgcgtt aaaaattgtt
     2761 cgccgggtaa ggtaagttgc gccccctgac gaccacgctc aaacaagcga gcgcccgtca
     2821 gctgctccag ttcattcaat gtattagaga gcgcaggttg actcaaatta agggtttcag
     2881 ctgcgcgccc caaagttcct tgttgtgcga cagctacgaa tgtatgaagg tggcgcaaac
     2941 ggatgcgctg actaaacaga ctattttttt ccataagcga tgttaaaaac gaagcggtgt
     3001 cgctgacaag tgaagttgtt tgattatgat aacttgattg caaaatatta ttaacaatta
     3061 aagcaattat gttacagcaa aatggataat attgatgttt tcgcggcgag atcacagttt
     3121 gtaaattctt cccgccagag tgaatgcggt tacctacact ccagattact gaccactgga
     3181 ggcagacact atggcgaaca gcatcacggc ggatgagatt cgggaacagt tttcgcaggc
     3241 aatgtcagcc atgtaccagc aagaagttcc gcaatatggc acgctgctgg aactggtagc
     3301 tgatgtgaat ctggctgtgc tggaaaacaa tcctcaactg cacgaaaaaa tggtaaatgc
     3361 agacgagctg gcgcgactga atgttgaacg tcatggggcg attcgcgttg ggactgcaca
     3421 agagcttgct actcttcggc ggatgtttgc cattatgggg atgtacccgg tgagctatta
     3481 cgatctctcg caggcagggg tgccggtaca ttcgacagca tttcggccca ttgatgatgc
     3541 ttctctggcg cgtaatccct tccgcgtttt tacatcctta ctccgccttg agcttatcga
     3601 gaacgaaatt ttgcgccaga aagcggcgga gattctgcgt cagcgcgata tcttcacccc
     3661 acgttgtcga caactgttag aggaatatga gcagcagggc ggttttaacg aaacacaggc
     3721 acaggagttt gtgcaggaag ccctggaaac gtttcgctgg caccagttag caacggtaga
     3781 tgaagaaacc tatcgcgcct tgcacaacga acatcggttg attgctgatg tggtctgttt
     3841 tcctggatgc catatcaacc acctgacgcc acgtacgctg gatattgacc gggtgcagtc
     3901 gatgatgcct gaatgcggaa ttgaacccaa aattctcatc gaggggccgc cgcgccgcga
     3961 ggtaccgatt ttactacgcc agaccagctt taaagcactg gaagagacgg tgttgtttgc
     4021 ggggcagaaa cagggcacgc ataccgcgcg ctttggtgaa attgagcagc gtggcgtggc
     4081 attaacgccg aaagggcgac aactgtatga tgatcttctg cgtaacgctg gaaccgggca
     4141 ggataatctc actcaccaaa tgcatttaca ggaaaccttc cgcacttttc ctgacagtga
     4201 gtttttaatg cgtcagcaag ggttggcatg gttccggtac cgtctgacgc cttcgggtga
     4261 ggcgcatcgt caggcgattc atcctggaga cgatccacag cccttaattg aacgtggttg
     4321 ggtagtggcg caacccatca cctatgaaga tttcttgccc gttagcgcgg cggggatctt
     4381 ccagtcaaat ctgggtaatg aaacgcagac acgcagtcac ggtaatgcca gtcgcgaagc
     4441 atttgagcag gcgttgggtt gtccggtttt ggatgagttc cagctttacc aggaagcgga
     4501 agaacgcagt aaacgtcgct gtggtttgct ttaaattctg accatccgcc tttgcaaaaa
     4561 tttgcctgat tttacaaacg aatcaggctc atcccatcga cataaaaaaa atgccgattt
     4621 atgcatattc tctcagttca acaattggat tattaataaa tattgtctag agtgagcggt
     4681 cataaataag cactttcttg ccgctgaaaa cgaccagcgc gggaccattc acaacaccag
     4741 aaggactcac tttcaggtat ggatcgtaga cgatttatta aaggttcaat ggctatggcc
     4801 gccgtgtgcg gtaccagcgg cattgcttct cttttttctc aggcagcatt cgcggcagat
     4861 tctgatattg ccgacgggca aacccagcgt tttgacttct ccattctaca gtcaatggcg
     4921 cacgacttag cgcaaacagc gtggcgtggt gcgcctcgtc cgttacctga cacgctggcg
     4981 acaatgacgc cgcaggctta taacagtatt caatacgacg ccgaaaaatc gctctggcat
     5041 aacgttgaga accgtcaact ggacgctcag ttcttccata tgggaatggg attccgtcgc
     5101 cgcgttcgta tgttttctgt agatccagca acacatctgg cgcgtgaaat tcactttcgc
     5161 ccggagttgt tcaaatacaa cgatgcaggt gttgatacca aacaattaga agggcaaagc
     5221 gatctcggct ttgccggttt tcgcgtgttt aaagcccccg aactggcgcg ccgtgatgta
     5281 gtatcatttc tcggcgcgag ttatttccgc gccgttgatg atacatatca atacggtttg
     5341 tcggcccgcg gcctggcgat cgacacttac accgacagta aagaagagtt ccccgacttt
     5401 accgccttct ggtttgatac ggtaaaaccg ggggcaacta cctttaccgt ttatgcgttg
     5461 ctcgatagcg ccagcattac tggtgcctat aagttcacta tccattgtga gaaaagtcag
     5521 gtgattatgg atgtggaaaa tcacctgtat gcgcgcaaag acattaaaca gctgggcatt
     5581 tcgccgatga ccagtatgtt cagctgcggt actaatgaac gtcggatgtg cgatacaatt
     5641 catccgcaaa ttcatgactc tgatcgtctg tccatgtggc ggggcaacgg cgagtggatt
     5701 tgccgtccgc tgaataatcc gcaaaaattg cagttcaatg cttacaccga caacaacccg
     5761 aaagggtttg gtttattgca actggatcgt gacttctccc attatcagga cattatgggc
     5821 tggtataaca aacgcccaag tctgtgggtg gaaccgcgta acaagtgggg taagggcacc
     5881 atcggcctga tggaaatccc aacaacgggc gaaacgctgg ataacattgt ctgcttctgg
     5941 cagccagaaa aagctgtaaa agcaggtgat gagtttgcat tccagtatcg tctgtactgg
     6001 agtgcgcaac cgcctgttca ttgcccatta gcgcgcgtta tggcgacgcg taccggcatg
     6061 ggcggtttcc cggaaggttg ggcgccaggt gaacactatc ccgaaaaatg ggcgcgtcgt
     6121 tttgccgtcg atttcgttgg tggtgatctg aaagctgccg cgccaaaagg cattgagccg
     6181 gtgattacgc tttccagtgg ggaagcgaag caaatcgaaa ttctctatat tgaacccatc
     6241 gatggttatc gtattcagtt tgactggtat ccgacttcgg actccactga tccggtcgat
     6301 atgcggatgt atctacgttg tcagggggac gctatcagtg aaacatggct gtatcagtat
     6361 ttcccgccag cgccggataa acgtcagtat gttgacgacc gcgtgatgag ttaatcgttt
     6421 tttcttcggc accttcttcg ggaggtgccg tctggttaaa cacgatcccg ctcgcatttt
     6481 tccctaagtt aaatgagtaa tctgatggtg tgtatttcag atacaccgtg tcagccacta
     6541 acagggagtg cgtatgtttc cagaataccg agatttaata tcccgtctga aaaacgaaaa
     6601 tcctcgcttt atgtccttgt tcgataaaca caataaactt gatcatgaaa ttgccagaaa
     6661 ggaaggttcc gacggtcgag ggtacaatgc ggaagtggtc cgcatgaaaa aacaaaagct
     6721 acagttaaaa gatgagatgc tcaaaatcct gcagcaggag agcgtcaaag aggtgtaaac
     6781 tttcctaagc cgccagccag gcggcttttt taacaacagc atggattgac tggagataag
     6841 atgactgaaa cgataaaagt aagcgaatca cttgaattac atgctgttgc ggaaagtcac
     6901 gtcacacctc tttatcagtt aatctgtaaa aataaaacct ggttacagca gtcgctaaac
     6961 tggccgcagt tcgttcagag tgaagaggac acgcgaaaaa cggtgcaggg taatgtgatg
     7021 ttgcatcaac gcggctatgc caaaatgttc atgattttca aagaagatga acttatcggc
     7081 gttatctcgt ttaatcgtat tgaaccactg aataaaactg ctgaaatcgg ctactggctg
     7141 gacgaatctc atcaggggca ggggatcatt tctcaggcgc tgcagacatt gattcatcat
     7201 tacgctcagt ctggtgaact tagacgcttc gtgatcaaat gtcgggtgga caatccgcaa
     7261 agcaaccagg tcgctttgcg caatggtttt atccttgaag gttgcctgaa acaggctgag
     7321 ttcctgaatg atgcctatga tgatgtgaac ttatacgcgc gtattatcga ttcataataa
     7381 ccctgccagc ggcgttcgcg taatgcgctc ctcgccgtta atgacttttg gaccacggac
     7441 atgaaccgtg tcgccatcaa aggcttcaac gacggcgtgg tcggttaact ccacatggtt
     7501 ttcaattatc accgcacctg taatacggct ctcaccttgt attacaacgt gctcgtcgag
     7561 cagaatcggt ccaccacgta ccacggcatt tccgccaatc aagacatgat gttttaacac
     7621 acaattacct tccacaatgg catattccgc cacctgcgaa ctgtaatgaa tcgtcggaat
     7681 ggcatcttct tctatgccag ctttcacctg cgcgtgaccg tagactttag cgcaatcgca
     7741 tagccagacg ttgttctctt cattaccttc aacactggca aaatcaaaaa cttcggcgcg
     7801 atgttcaata aaagcatacc ggacgacggc atcgccataa atttgtgcct ggtggacaat
     7861 acgcgaggcg ctaacccttg cgcgatcata aatttggagc aggagttgat ggtcgggcgt
     7921 taagccttgt gcggcgacga tcatagaatg ttgatcaatt aaggcgtgac caaatattct
     7981 acattgcacg taaactaatg agtcatgaat ggtgacactg tcacttatat atgcaccctg
     8041 gctgatttca ctattatcga tccagacgtt atccgttgcg taaacctctc cccacagcac
     8101 gctggtgccg gtgatacgcg tattaccgga aatcaccgtt ccgccgaacg caatagcatt
     8161 ctggtcgtaa atccagcaat ttccttcctg tgctaacacc gtttcgcgat cgatccagcc
     8221 gccagcggtt cctgctatca catcgttaaa atcgctaatg gcgataatct gccgtaataa
     8281 cacacttttt ttagtgccat cttcctgata actaaaggct cgctgttctt cacttaggcg
     8341 atatttgcgc atagcgtttt cccacaggtg acttactata accgtagcaa attctgcggc
     8401 tctggctatg ctcgagaaaa ttccataaaa tgcatttcaa atatacttta taaattaaac
     8461 aaaatgagta agaagatgca gagcgataaa gtgctcaatt tgccggcagg ctactttggt
     8521 attgtgttgg ggacgatagg gatgggattt gcctggcgct atgccagcca ggtttggcag
     8581 gtcagccact ggttagggga tgggctggtg attctggcga tgatcatctg gggattattg
     8641 actagcgcat ttattacccg actcatacgc tttccgcata gcgtgctggc ggaagttcgc
     8701 catccagtgc tgagcagttt tgtgagtttg tttccggcaa cgacgatgct ggtggcgatt
     8761 ggttttgttc cgtggtttcg cccactggcg gtgtgcatgt tcagttttgg tgtcgtggtt
     8821 cagttggctt atgccgcctg gcaaactgcg ggattatggc gcggatctca ccctgaagaa
     8881 gctacctcgc ctggactgta tctgccgaca gttgccaaca actttatcag cgcaatggcc
     8941 tgtggtgcgt tgggctacac cgacgccggt ctggtgtttt taggcgcagg cgttttctca
     9001 tggctaagcc ttgaaccggt gatcttgcag cgtctgcgca gttcgggaga attacccacg
     9061 gcactgcgga catcactcgg cattcagctc gctcctgcgc tggtggcctg tagtgcctgg
     9121 ctgagcgtca acggcggcga gggtgacacg ctggcgaaaa tgcttttcgg ttatggactg
     9181 ctgcaactgc tgtttatgct acgtctgatg ccatggtatc tctcccagcc atttaatgct
     9241 tcattctgga gtttctcgtt cggcgtatct gcactggcaa ccaccggttt gcatctgggg
     9301 agtggcagcg ataatggatt tttccatacg ctggcggtgc cgctgtttat ctttaccaat
     9361 tttattattg caatactgct catccgtact tttgcgcttc tgatgcaggg aaaattgtta
     9421 gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca aagaatgatc attcgtgacg
     9481 aaaactattt tactgataaa tatgaattaa cccgcacaca ctctgaagta ctggaagcgg
     9541 tgaaagtggt taaaccgggt aaaacgctgg atctgggctg tggcaatggt cgtaacagtc
     9601 tttacctggc agccaatggt tatgatgttg acgcatggga taaaaatgcc atgagtatcg
     9661 ccaacgtcga gcgcattaaa tccattgaaa atctggataa tttacacacc cgggtcgttg
     9721 atctgaataa cctcacattt gatggacagt acgattttat tctttcgact gtggtgctga
     9781 tgttccttga ggctaaaacc atcccagggt tgattgccaa tatgcaacgt tgcactaaac
     9841 ctggtggtta caacctgatt gtggcggcga tggataccgc tgattatcca tgtaccgtcg
     9901 gcttcacgtt tgccttcaaa gagggagaat tacgtcgata ttacgaaggc tgggagaggg
     9961 tgaaatacaa tgaagacgtc ggcgagctgc accgcaccga cgccaacggt aatcgtatta
    10021 aactgcgttt cgccacgatg ctggcacgta aaaaatgacc cggtaagcac aaaacgcgtg
    10081 aaaattcccc acgctgagat gatttactgt tcttcttttc ggtaagcata ttttttatcg
    10141 aagggatgtg aaattaatca cagtagtcga agttttttag cagcttaact tactgaaatt
    10201 taactactga tgattgactt agcccctttt tcggcattga ctatgtcgtc tgaaaagggg
    10261 ctgaaaaatt tattttcacc aacacttttt ttgccacaac acgaagcggc gctttttgct
    10321 ataacttaga aagtaatata atcatctcag gaaactattc atgcgtacca catcatttgc
    10381 gaaagttgca gctttatgcg gcttgttggc tctgtctggt tgtgcctcta aaatcaccca
    10441 gccagataaa tataacaatt actctgattt aaaagaaaca acctcagcaa caggtaaacc
    10501 tgttttacgt tgggtagacc cgagttttga tcaaagcaaa tatgacagca tcgtctggaa
    10561 cccaatcact tattatccgg taccaaaacc ttctacccag gtagggcaga aagttctgga
    10621 taaaattttg aactatacca acaccgaaat gaaagaagcg atagcgcagc gtaaaccagt
    10681 ggttaccacc gctgggccgc gtagtctgat tttccgtggg gccattaccg gtgtagatac
    10741 cagcaaagaa gggctgcaat tctatgaagt ggttcctgtt gcattagtgg ttgcggggac
    10801 gcaaatggct acaggccacc gtaccatgga tactcgcctc tattttgaag gtgagctgat
    10861 tgatgcagcg actaataaac cggttatcaa agtcgttcgt cagggcgaag gtaaagacct
    10921 gaataacgaa agtacgccaa tggctttcga aaatattaaa caagttattg atgacatggc
    10981 gaccgatgcc accatgtttg acgttaacaa aaagtagttc cagacgcgcc atcgttagat
    11041 ggcgcttttt atccggtgcg ccgtaaaacc ccatccttca gggcggggat ataaggcgcg
    11101 gttttccacc tgaccaggtg tttgctgttg ttcaatgtat tggcggatga cggatattgg
    11161 cgcacctccg cagctactgg caaaatagcc aggactccac aaaacgcctt tgtagtaata
    11221 cctgactgca atatctggtc gatctcgtcg cagtaaccta cccgatacgc ctttgaggct
    11281 gtttaccaga ctggatatcg ccagtttggg aggttagtta attaacaaat ggacgtgatc
    11341 tggttcgcca tccat
//
LOCUS       DAZXUL010000128        11110 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZXUL010000128 DAZXUL010000000
VERSION     DAZXUL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.128"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACH3L1_003470"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACH3L1_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFT4078079.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACH3L1_003471"
     tRNA            complement(621..695)
                     /locus_tag="ACH3L1_003471"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="ACH3L1_003472"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="ACH3L1_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="ACH3L1_003473"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="ACH3L1_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFT4078080.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /gene="yfdF"
                     /locus_tag="ACH3L1_003474"
     CDS             complement(2932..3990)
                     /gene="yfdF"
                     /locus_tag="ACH3L1_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFT4078081.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="ACH3L1_003475"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="ACH3L1_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFT4078082.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="ACH3L1_003476"
     CDS             6068..6352
                     /locus_tag="ACH3L1_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFT4078083.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="ACH3L1_003477"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="ACH3L1_003477"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFT4078084.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="ACH3L1_003478"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="ACH3L1_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFT4078085.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="ACH3L1_003479"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="ACH3L1_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFT4078086.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2778 a         2542 c         2747 g         3043 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtcagttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtga aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
    11101 ttaaggatgt
//
LOCUS       DAZXUL010000129        11050 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZXUL010000129 DAZXUL010000000
VERSION     DAZXUL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.129"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..75)
                     /gene="ytiD"
                     /locus_tag="ACH3L1_003480"
     CDS             complement(<1..75)
                     /gene="ytiD"
                     /locus_tag="ACH3L1_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFT4078087.1"
                     /translation="MADYAEINNFPPELSSSGDKYFHLR"
     gene            409..1239
                     /locus_tag="ACH3L1_003481"
     CDS             409..1239
                     /locus_tag="ACH3L1_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFT4078088.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="ACH3L1_003482"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="ACH3L1_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFT4078089.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="ACH3L1_003483"
     CDS             complement(2368..3828)
                     /locus_tag="ACH3L1_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFT4078090.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="ACH3L1_003484"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="ACH3L1_003484"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFT4078091.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="ACH3L1_003485"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="ACH3L1_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFT4078092.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="ACH3L1_003486"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="ACH3L1_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFT4078093.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8392)
                     /locus_tag="ACH3L1_003487"
     CDS             complement(7949..8392)
                     /locus_tag="ACH3L1_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4078094.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="ACH3L1_003488"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="ACH3L1_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFT4078095.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..>11050)
                     /gene="fimD"
                     /locus_tag="ACH3L1_003489"
     CDS             complement(9005..>11050)
                     /gene="fimD"
                     /locus_tag="ACH3L1_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFT4078096.1"
                     /translation="NRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKN
                     KWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAP
                     VIHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQI
                     FTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQ
                     LADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTN
                     IQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQL
                     TVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKG
                     RDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNL
                     SYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHA
                     NGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTN
                     TLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQ
                     SSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2686 a         2827 c         2683 g         2854 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgtgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgacgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     3901 ggtgggtatt aacggataaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt tgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc gtcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaata gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccgatt
//
LOCUS       DAZXUL010000130        11036 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZXUL010000130 DAZXUL010000000
VERSION     DAZXUL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11036
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.130"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            157..504
                     /locus_tag="ACH3L1_003490"
                     /pseudo
     CDS             157..504
                     /locus_tag="ACH3L1_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            557..952
                     /locus_tag="ACH3L1_003491"
     CDS             557..952
                     /locus_tag="ACH3L1_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFT4078097.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="ACH3L1_003492"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="ACH3L1_003492"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFT4078098.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="ACH3L1_003493"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="ACH3L1_003493"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFT4078099.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACH3L1_003494"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACH3L1_003494"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFT4078100.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="ACH3L1_003495"
     CDS             complement(5323..6423)
                     /locus_tag="ACH3L1_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFT4078101.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="ACH3L1_003496"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="ACH3L1_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFT4078102.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="ACH3L1_003497"
     CDS             complement(7571..8137)
                     /locus_tag="ACH3L1_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFT4078103.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="ACH3L1_003498"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="ACH3L1_003498"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFT4078104.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(10128..10172)
                     /locus_tag="ACH3L1_003499"
     CDS             complement(10128..10172)
                     /locus_tag="ACH3L1_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078105.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            10263..10751
                     /locus_tag="ACH3L1_003500"
     CDS             10263..10751
                     /locus_tag="ACH3L1_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFT4078106.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(10869..10937)
                     /locus_tag="ACH3L1_003501"
                     /pseudo
     CDS             complement(10869..10937)
                     /locus_tag="ACH3L1_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
BASE COUNT         2690 a         2814 c         2634 g         2898 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctt tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgccctg aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc
    10981 agatcatcgt ttccgatgga agcataataa gctttttctg cttctgccgg aggagt
//
LOCUS       DAZXUL010000131        10856 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZXUL010000131 DAZXUL010000000
VERSION     DAZXUL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10856
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.131"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            109..1008
                     /gene="lysO"
                     /locus_tag="ACH3L1_003502"
     CDS             109..1008
                     /gene="lysO"
                     /locus_tag="ACH3L1_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFT4078107.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1152..2804
                     /gene="hcp"
                     /locus_tag="ACH3L1_003503"
                     /gene_synonym="priS"
     CDS             1152..2804
                     /gene="hcp"
                     /locus_tag="ACH3L1_003503"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFT4078108.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            2816..3784
                     /gene="hcr"
                     /locus_tag="ACH3L1_003504"
     CDS             2816..3784
                     /gene="hcr"
                     /locus_tag="ACH3L1_003504"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFT4078109.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3917..5635
                     /gene="poxB"
                     /locus_tag="ACH3L1_003505"
     CDS             3917..5635
                     /gene="poxB"
                     /locus_tag="ACH3L1_003505"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFT4078110.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5672..6673
                     /gene="ltaE"
                     /locus_tag="ACH3L1_003506"
     CDS             5672..6673
                     /gene="ltaE"
                     /locus_tag="ACH3L1_003506"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFT4078111.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6684..8114
                     /locus_tag="ACH3L1_003507"
     CDS             6684..8114
                     /locus_tag="ACH3L1_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFT4078112.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8213..9226
                     /locus_tag="ACH3L1_003508"
     CDS             8213..9226
                     /locus_tag="ACH3L1_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFT4078113.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9223..10053)
                     /gene="amiD"
                     /locus_tag="ACH3L1_003509"
     CDS             complement(9223..10053)
                     /gene="amiD"
                     /locus_tag="ACH3L1_003509"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFT4078114.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10050..10373)
                     /locus_tag="ACH3L1_003510"
     CDS             complement(10050..10373)
                     /locus_tag="ACH3L1_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFT4078115.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10584..>10856)
                     /locus_tag="ACH3L1_003511"
     CDS             complement(10584..>10856)
                     /locus_tag="ACH3L1_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFT4078116.1"
                     /translation="VKVAADAFLEIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYIN
                     DKSIEILLLSCFAAADQKAIQRYRPQSQDGEDFRSHLQYKLPLSAH"
BASE COUNT         2475 a         2881 c         2871 g         2629 t
ORIGIN      
        1 ccctctgatg cataacattt ccttttaatt gcaactttac tcgcccttcc gctttcagta
       61 gtgtgtcatc gcgcatttcg acttcttctt tgcaaggact ggattttcat gttttctggg
      121 ctgttaatca ttctggttcc cctgattgtg ggttacctca ttccgcttcg ccaacaagct
      181 gcgttaaaag ctattaatca gctattaagc tggatggttt accttattct cttttttatg
      241 ggtatcagtc tggcgtttct cgataacctc gccagtaacc tgttggcgat tctgcattat
      301 tctgccgtca gtattaccgt tattttactg tgtaatattg ccgccctgat gtggctggag
      361 cgaggcctgc cgtggcgcaa ccaccatcag caagaaaaac tcccgtcgcg tattgcgatg
      421 gcgctggagt cgctaaaact gtgcggcgta gtagtgattg gttttgccat tggtctgagt
      481 ggactggctt tcttacaaca cgcgaccgaa gccagtgaat acacgttaat tttgctactt
      541 ttcctcgttg gtattcagtt gcgcaataat ggcatgacct taaagcagat tgtccttaat
      601 cgccggggaa tgattgtcgc cgtggtggtg gttgtcagtt cattaattgg tggtttaatt
      661 aacgccttta ttcttgatct ccccatcaat accgcgctgg caatggcctc cggtttcggc
      721 tggtattctc tttccggtat tttattgacc gaatcttttg gtccggtaat cgggagcgcg
      781 gcgtttttta atgatctggc ccgtgaactg attgctatta tgttgatccc tgggctgatt
      841 cgccgcagcc gctctactgc actgggctta tgcggtgcca catcaatgga tttcaccctg
      901 cccgttcttc aacgtactgg cgggctggat atggtcccgg cggcaattgt tcacggtttt
      961 attcttagcc tgttagtgcc gatcctcatc gcctttttct ctgcgtaata cctctctggc
     1021 ggtagttccc tgccgccaaa attgcgctaa atcaatctcc cttaaagttg catgaaaaat
     1081 cccttttatc cccgcgttaa gcgtcttacc cttaaacatg tatattaaat ataactttaa
     1141 aaggtgtgat catgttttgt gtgcaatgtg aacaaactat ccgtactccg gcagaaaacg
     1201 gctgctcata cgcgcagggg atgtgtggta aaacggcgga aacttctgac cttcaggatt
     1261 tactcatcgc ggcgctgcaa gggctttctg cctgggcggt aaaagcgcgt gaatacggca
     1321 tcatcaacca cgatgttgac agcttcgcgc cacgtgcctt tttctcaacc ctgaccaacg
     1381 ttaacttcga ttctccgcgt attgtcggct acgctcgtga agcgattgcc ttgcgcgagg
     1441 cgctgaaagc acaatgcctg gctgtagatg ccaacgcgcg cgtcgataac ccgatggctg
     1501 acctgcaact ggtgagcgat gatctcggcg agctgcaacg tcaggcagca gaatttaccc
     1561 ctaacaaaga taaagcggcg attggcgaaa acattctcgg cctgcgtctg ctgtgcctgt
     1621 atggcctgaa aggtgcggcg gcctatatgg aacacgcgca tgttctcggt caatacgaca
     1681 acgatattta tgcccagtac cataaaatca tggcgtggct ggggacctgg cctgccgata
     1741 tgaacgcgct tcttgagtgt tcaatggaaa tcggccagat gaacttcaaa gtgatgagca
     1801 ttctggatgc aggcgaaacc ggtaaatacg gtcacccgac gccaacccag gtcaacgtca
     1861 aagcgacggc gggtaaatgc attctgattt ccggtcacga tctcaaagat ctctacaacc
     1921 tgctggaaca gaccgaaggc acgggcgtta atgtctacac ccacggcgaa atgctgcctg
     1981 cgcatggcta cccggagctg cgtaaattca agcatctggt cggtaactac ggcagcggct
     2041 ggcagaatca gcaagtggag ttcgctcgtt tccctggccc catcgtgatg acctcgaact
     2101 gcatcatcga cccaaccgta ggcgcttatg acgatcgtat ctggacccgc agcatcgttg
     2161 gctggcctgg cgtgcgtcat ctggatggcg aagatttctc tgcggtcatc gcccaggcgc
     2221 aacaaatggc gggcttcccg tacagcgaaa ttcctcacct gatcaccgtg ggctttggtc
     2281 gccagacgct gcttggtgca gccgatacgc tgattgatct ggttagccgt gaaaaactgc
     2341 gtcatatctt cctgcttggt ggctgtgacg gcgcacgcgg cgagcgccac tacttcaccg
     2401 atttcgccac cagcgtgccg gatgactgcc tgatcctgac cctcgcctgt ggtaaatatc
     2461 gctttaacaa acttgagttt ggcgatatcg aaggtctgcc gcgtctggta gatgcaggtc
     2521 aatgtaacga tgcttactca gcaattattc tggctgtcac tctggcagag aaactgggct
     2581 gtggtgtgaa cgatctgccg ctgtcgctgg tgctctcctg gtttgaacag aaagcgattg
     2641 tcattctgct gacgctgctt tctctgggcg tgaaaaatat cgtcaccggc ccgactgcac
     2701 ctggtttcct gacaccagac ctgctggcgg tgctgaacga gaaattcggc ctgcgttcta
     2761 tcaccactgt tgaagaagac atgaagcaac tgttgaacgc gtaaggagtt tgtcgatgac
     2821 gatgccaacg aatcaatgcc cgtggcggat acaggttcat cacattacgc aagaaacgcc
     2881 ggatgtgtgg acgatttccc tgatttgcca cgatcactac ccatatcgcg ccgggcaata
     2941 tgcactggtc agcgtgcgaa actcagcgga aacgctgcgt gcttacacca tttcctccac
     3001 gccaggcgtg agtgaatata tcaccctgac cgtgcggcgg attgatgacg gtgtcggctc
     3061 ccagtggctg acgcgcgatg taaaacgcgg tgattatctc tggctttcgg acgcgatggg
     3121 ggaatttacc tgcgacgata aagcagaaga taaattcctg ttgctggcgg caggctgcgg
     3181 cgtcacgccg attatgtcga tgcgtcgctg gcttgcgaag aaccgtccac aggccgatgt
     3241 gcgggtgatc tacaacgtgc gtacgccgca ggatgttatt ttcgccgatg agtggcgtaa
     3301 ctatccggta acgctggtgg cagaaaataa cgttaccgaa ggctttatcg ctggtcgcct
     3361 cactcgcgaa ctgctggcag atgtacctga cttagcttca cgtaccgtga tgacctgcgg
     3421 cccggctccg tatatggatt gggtagagca ggaagtgaaa gcgctcggcg tgacgcgttt
     3481 ctttaaagag aaattcttca ccccagtagc agaagcggcg accagcggtc tgaaattcac
     3541 caaactgcaa ccggcacgag aattttacgc cccggttggc accacgctac tggaggcgct
     3601 ggaaagcaat aacgttccgg ttgtcgctgc ctgccgtgcg ggtgtttgcg gctgctgtaa
     3661 gacaaaagtg gtttccggtg aatatacggt gagcagtaca atgacgctga ccgacgccga
     3721 aatcgctgaa ggttacgtac tggcctgctc ctgccatccg cagggggatt tggttctcgc
     3781 ataatcgcct tatgcccgat gatattcctt tcatcgggct atttaaccgt tagtgcctcc
     3841 tttctctccc atcccttccc cctccgtcag atgaactaaa cttgttaccg ttatcacatt
     3901 caggagatgg agaaccatga aacaaacggt tgcagcttat atcgccaaaa cactcgaatc
     3961 ggcaggcgtg aaacgcatct ggggagtcac gggcgactct ctgaacggtc ttagcgacag
     4021 tcttaatcgc atgggcacca tcgagtggat gtccacccgc catgaagaag tggcggcgtt
     4081 tgccgctggc gctgaagcac aactttcagg agaactggcg gtctgtgccg gatcgtgcgg
     4141 cccaggcaac ctgcacttga tcaacggcct attcgattgc caccgcaata acgttccggt
     4201 actggcgatt gccgctcata ttccctccag cgaaattggc agcggctatt tccaggaaac
     4261 ccacccacaa gagctattcc gcgaatgtag tcactattgc gagctggttt ccagcccgga
     4321 gcagatccca caagtgctgg caattgctat gcgcaaagcg gtgcttaacc gtggcgtttc
     4381 cgttgttgta ttaccgggcg acgtggcgtt aaaacctgcg ccagaagggg caaccacgca
     4441 ctggtatcat gcgccacagc cagtcgtgat gccggaagaa gaagagttac gcaaactggc
     4501 gcaactgctg cgttattcca gcaatatcgc cctgatgtgt ggcagcggct gcgcgggggc
     4561 gcataaagag ttagttgagt ttgccgggaa aattaaagca cctattgttc atgccctacg
     4621 cggtaaagag catgtcgaat acgataatcc gtatgatgtt ggaatgaccg ggttaatcgg
     4681 attctcgtca ggtttccata ccatgatgaa cgccgacacg ttagtgctac tcggtactca
     4741 gttcccctac cgtgcgttct acccgaccga tgccaaaatt attcagattg atattaatcc
     4801 agccagcatc ggcgcgcata gcaaggtaga tatggcgctg gtcggcgata tcaaatcaac
     4861 cctgcgcgca ttgctgccac tggtggaaga aaaaaccgat cgcaagtttc tggataaagc
     4921 gctggaagat taccgcgatg cccgcaaagg gctggacgat ttagctaaac cgagcgagaa
     4981 agccattcac ccgcaatatc tggcgcagca aattagtcat tttgccgccg atgacgccat
     5041 ctttacctgt gacgtcggca cgccaacggt atgggcggca cgttatctga aaatgaacgg
     5101 caagcgtcgc ctgttaggtt cgtttaacca cggttcgatg gctaacgcca tgccgcaggc
     5161 gttgggtgcg caggcaaccg agccggaacg tcaggtggtc gccatgtgcg gcgatggcgg
     5221 ttttagcatg ttgatgggcg atttcctctc agtagtgcag atgaaactgc cggtgaaaat
     5281 tatcgtcttt aataacagcg tgctgggctt tgtggcgatg gagatgaagg ccggaggtta
     5341 cctgacagac ggtactgagc tgcacgacac caactttgcc cgcattgccg aagcgtgcgg
     5401 cattacgggt atccgtgtag aaaaagcctc tgaagttgat gaagccctgc aacgcgcctt
     5461 ctctatcgac ggtccggtgt tggtggatgt ggtggtcgcc aaagaagaat tagccattcc
     5521 accgcagatc aaactcgaac aggccaaagg ttttagcctg tatatgttgc gcgcaattat
     5581 cagcggacgc ggcgatgaag tgatcgaact ggcgaaaacg aactggctaa ggtaaaaagg
     5641 gtggcatttc ccgtcataat aaggacatgc catgattgat ttacgcagtg ataccgttac
     5701 ccgaccgagc cgcgccatgc tcgaagcgat gatggccgcc ccggttgggg acgacgttta
     5761 cggagacgac cctaccgtta atgctctgca ggactatgca gcagagcttt ccggtaaaga
     5821 agccgccatt tttctgccta ccggcactca ggccaacctg gtcgctctgc tcagtcactg
     5881 cgaacgtggc gaagagtata ttgtcggtca ggccgcgcat aactatctgt ttgaagccgg
     5941 tggcgcagcg gtgctgggca gtattcagcc gcaacccatc gacgcggctg ccgacggcac
     6001 gctaccgctg gataaagtgg cgatgaaaat caaacccgac gatatccatt tcgcccgcac
     6061 caaattactc agtctggaaa acacccacaa cggcaaagtg ctgccgcgtg aatacctgaa
     6121 agaagcatgg gaatttaccc gcgagcgcaa tctggcgctg catgttgacg gtgcgcgcat
     6181 ctttaatgcc gtggtggctt acggctgcga actgaaagag atcacgcaat attgtgattc
     6241 gttcaccatt tgcctgtcga aaggtcttgg gacgccagtc ggttcattac tcgtcggtaa
     6301 tcgtgattac attaaacgtg ccatccgctg gcggaaaatg gccggtggcg ggatgcgtca
     6361 gtccgggatt ctggctgccg ccgggatgta tgccctggaa aataacgtcg cacggttgca
     6421 ggaagatcac gacaacgccg cctggatggc ggagcaactg cgtgaagcag gcgcggatgt
     6481 gatgcgtcag gacactaata tgctgtttgt tcgcgtcggg gaagaaaatg ctgccgcgtt
     6541 aggcgaatac atgaaagcga gaaacgtgct gattaacgcc tcgccgattg tccgcctggt
     6601 gacgcatctt gacgtctcgc gcgaacaact ggcggaagtc gccgcccact ggcgcgcatt
     6661 cctggcgcgt taaggagaga gacgtgccgc aacgcatttt agttctcggt gccagtggct
     6721 acattggtca gcatctggtg cgcacactca gccagcaagg gcatcagatc ctagcggcgg
     6781 cacgtcatgt cgacaggctt gcaaagctgc aactggcaaa cgtcagttgc cataaagtcg
     6841 atctcagctg gccggataac cttccggccc tgttgcagga tatcgatacc gtctattttc
     6901 tggtgcacag catgggcgaa ggcggcgatt ttatcgctca ggagcgccag ctggctctca
     6961 acgtccgcga tgcgctacgt gaagtaccag ttaagcaatt aatctttctc agttcgttgc
     7021 aggccccgcc acatgagcag tcggatcatc tgcgagctcg tcaggctacg gcggacattc
     7081 ttcgtgaagc gaatgtacca gtgaccgaac ttcgggccgg aattatcgtt ggcgcaggtt
     7141 cagcggcgtt cgaagtcatg cgcgatatgg tctacaacct gccagtgtta acgccgccac
     7201 gctgggtacg ttcacgcacc acgcccatcg cgctggaaaa cttgctgcac tatctggtgg
     7261 cgctgttaga ccatccagcc aacgaacacc gcatcttcga agccgccgga ccagaggtgc
     7321 tcagttatca gcaacagttt gaacatttta tggcggtgag cggtaagcgc cgctggttga
     7381 tccccatccc cctccccacc cgctggattt cggtgtggtt tctcaatgtg attacttccg
     7441 taccgcccac caccgccagg gcgttgattc aggggctgaa acacgatctg ctggcagatg
     7501 ataccgcgct acgtgcactc atcccacaac ggctgatcgc tttcgatgac gcggtacgta
     7561 gcacgttgaa agaggaagaa aaactggtca actccagcga ctggggctac gacgctcagg
     7621 cctttgcccg ctggcgaccg gagtacggtt atttcgccaa acaggcgggg tttaccgtta
     7681 aaacgtccgc cagccttgcg gctttatggc tggtggtgaa ccaaatcggc ggtaaagagc
     7741 gttatttctt tggcaatatt ttgtggcaga cacgggcgtt gatggaccgc gcgatcggtc
     7801 ataatctggc gaaaggccgc ccggagcgcg aatatttaca gaccggcgat gcggtggata
     7861 gctggaaagt gattgtcgtt gaaccgaaaa agcaacttac gttgttattt ggcatgaaag
     7921 cgccggggct gggacgactg tgttttaccc tggaagataa aggtgactat cgtactatcg
     7981 atgtccgcgc tttctggcat ccgcacggta tgccggggct gttttactgg ttattgatga
     8041 tccccgcgca tctgtttatt tttcgcggaa tggcaaaaca aatcgccaga ctggcagaac
     8101 aaagcacaga ttaatcgaca aaacctgcgt tattctttca tgtttcccat tgcatcatgg
     8161 gggaaatcac ggaagaatgc gcacgtcatt ttctcaacaa cagtggattg atatgaaggt
     8221 actggttacc ggcgccacca gcggcttagg tcgaaacgcg gtagagtttt tatgccagaa
     8281 aggcatcagc gtgcgtgcga gcggtcgcaa cgaagcaatg ggtaaattgc tggaaaaaat
     8341 gggcgcagag tttgttccga cggatctgac cgagctggtt tcatcacaag ctaaagtgat
     8401 gctcgcgggc attgatacgc tgtggcactg ctccagcttt acctcaccct gggggacaca
     8461 acaggctttc gatctggcta acgttcgcgc cactcgccgc ctgggtgaat gggctgtcgc
     8521 ctggggtgtg cgtaacttta ttcatatctc ttccccctcc ctgtacttcg attatcacca
     8581 ccatcgcaat attaaagaag attttcgccc tcaccgcttc gccaacgagt ttgcccgcag
     8641 caaagcagcc agcgaagaag tgatcaatat gctttcgcag gcgaatccac aaacgcgctt
     8701 tactattctg cgcccacaaa gtctgttcgg accgcacgat aaagtcttta ttccccgtct
     8761 ggcgcatatg atgcaccact acggcagtat tctgttaccg catggcggca gtgcgctggt
     8821 cgatatgacc tactatgaaa atgccgtgca cgcaatgtgg ctggcaagcc aggaagcctg
     8881 cgataagcta ccttccgggc gtgtgtacaa catcaccaac ggcgagcatc gcacactgcg
     8941 cagcatcgtg cagaagctga tcgacgagtt gaatattgac tgtcgtattc gttccgtccc
     9001 ttacccgatg ctggatatga tcgcccgcag catggagcgt ttaggccgca agtcagcaaa
     9061 agagccgccg ctgacccact acggcgcctc caagttgaat tttgacttta cgctggatat
     9121 tacgcgtgcg caggaagagt taggttatca gccggtcatc accctggatg aaggcatcga
     9181 gaaaactgcc gcctggctac gcgaccacgg aaaactgccg cgctaatcct gcccgtattt
     9241 ctccagcaat gcttcggcaa tcgcctgagt ttctgcatcc gcttcgccgt tatataacgt
     9301 cgggcggaaa tgcatctgga atgccataat cacgcgccgc tgctcgcgcg gtgtcatatc
     9361 aggttttacg tcataaccgt agcgcgccaa aagctccagc aatgacgcgg tatctaccgg
     9421 tgtgtgcggc gcgcgcccgg caaggtaaaa gttcacccgc tgcgcatccg gccaggcacc
     9481 aatcccctgc tgcgccagtt gctgccaggg aaataatggc cccggatcgt ctttgcgctg
     9541 cggtgcgata tccgcatgtg ccactacgtt ttccggcttg atgtgataac gggcaataat
     9601 atctttcgcc agcggaataa gcgcctgaat ctgtgcctgt tcaaacgggg caaaatattt
     9661 cactccggat gatttttgcc agccacggtt ttccagctca atgccaatag aggtgtcgtt
     9721 aaggcgcgtt gccccgcgcc aggcgctaat ccccgcatgc caggccagtt cttgttccgg
     9781 caccagttgc cagatgcgcg gtttaccgtt gtatcgcggt ggcaccgcag ggatcagata
     9841 atgcgagctg acctgcttat cagtcagtgt cgccagcgag ctatcaaaat catctgcggt
     9901 gtagtggatc accagcactt taatgcgcgg atacgccgcc tgcgcctggt gtcgggtatc
     9961 aagctgatat ccctctttct cgacaatgcc tttttcgcct gcacaccctg ccagtaacag
    10021 agcggcagcg accagccaga agactcttct catcgacgcg ttttcaccgc cgtaccgctg
    10081 acgctaacca tcagcatact gccgttttgc ccgaccgttt cgtagtcgat atcaatacca
    10141 actacggcat cggcccccag cgcccgcgcc tgggagccta attcctcaaa ggcgatctcc
    10201 cgtgctttac gcagttcttt ttcatacgca ccggaacgtc cgccaacgat atcgcggata
    10261 ccggcaaaga aatcacggaa aatattggca cctaaaatcg cttcgccggt caccacaccg
    10321 caatattcaa cgatggtcag gccttccaga gttggggttg tagaaaattg catgatcgat
    10381 ctccttgttt gttaagcctt cgatctctaa agcattatcg gactgatatg ccatgttggg
    10441 aagaaggttt tgatcagttt aggaaatcat ctcattgtgt caaaaacacc cacttaagtc
    10501 tttacgatta tccgttgcat aacccgatat tggtttcatt atagggtacc gcttacctgt
    10561 cttattatgt tgcaccaaca acattaatga gcggaaagag gcaacttata ttgtaaatga
    10621 ctcctaaagt cttccccatc ttgtgactga ggtctatacc gctggattgc tttttggtca
    10681 gccgccgcaa aacaagaaag taataagatc tcaatgcttt tgtcattaat ataagcgaag
    10741 atcattcttg caaactcgta tataaacgcc cgatcctgag cgcttaatag tttaatacaa
    10801 tatttatttt taagagagaa aataatttca aggaaagcat ccgcagcaac cttcac
//
LOCUS       DAZXUL010000132        10746 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZXUL010000132 DAZXUL010000000
VERSION     DAZXUL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10746
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.132"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            110..1510
                     /locus_tag="ACH3L1_003512"
     CDS             110..1510
                     /locus_tag="ACH3L1_003512"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFT4078117.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            1666..3201
                     /gene="gadC"
                     /locus_tag="ACH3L1_003513"
     CDS             1666..3201
                     /gene="gadC"
                     /locus_tag="ACH3L1_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFT4078118.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            3332..4651
                     /locus_tag="ACH3L1_003514"
     CDS             3332..4651
                     /locus_tag="ACH3L1_003514"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFT4078119.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            5028..6098
                     /gene="dosC"
                     /locus_tag="ACH3L1_003515"
                     /pseudo
     CDS             5028..6098
                     /gene="dosC"
                     /locus_tag="ACH3L1_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            6098..6715
                     /locus_tag="ACH3L1_003516"
                     /pseudo
     CDS             6098..6715
                     /locus_tag="ACH3L1_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            6973..7305
                     /gene="ddpX"
                     /locus_tag="ACH3L1_003517"
                     /pseudo
     CDS             6973..7305
                     /gene="ddpX"
                     /locus_tag="ACH3L1_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            7319..8215
                     /gene="ddpC"
                     /locus_tag="ACH3L1_003518"
     CDS             7319..8215
                     /gene="ddpC"
                     /locus_tag="ACH3L1_003518"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFT4078120.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8212..9186
                     /locus_tag="ACH3L1_003519"
     CDS             8212..9186
                     /locus_tag="ACH3L1_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFT4078121.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            9179..10105
                     /locus_tag="ACH3L1_003520"
                     /pseudo
     CDS             9179..10105
                     /locus_tag="ACH3L1_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(10161..10592)
                     /gene="osmC"
                     /locus_tag="ACH3L1_003521"
     CDS             complement(10161..10592)
                     /gene="osmC"
                     /locus_tag="ACH3L1_003521"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFT4078122.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2616 a         2648 c         2709 g         2773 t
ORIGIN      
        1 ataaataaca ttaggatttt gttatttaaa cacgagtcct ttgcacttgc ttactttatc
       61 gataaatcct acttttttaa tgcgatccaa tcattttaag gagtttaaaa tggataagaa
      121 gcaagtaacg gatttaaggt cggaactact cgattcacgt tttggtgcga agtctatttc
      181 cactatcgca gaatcaaaac gttttccgct gcacgaaatg cgcgacgatg tcgcatttca
      241 gattatcaat gatgaattat atcttgatgg caacgctcgt cagaacctgg ccactttctg
      301 ccagacctgg gacgacgaaa acgtccacaa gttgatggat ttatccatta acaaaaactg
      361 gatcgacaaa gaagaatatc cgcaatccgc agccatcgac ctgcgttgcg taaacatggt
      421 tgccgatctg tggcatgcgc ctgcgccgaa aaatggtcag gccgttggca ccaacaccat
      481 tggttcttcc gaggcctgta tgctcggcgg gatggcgatg aaatggcgtt ggcgcaagcg
      541 tatggaagct gcaggcaaac caacggataa accaaacctg gtgtgcggtc cggtgcaaat
      601 ctgctggcat aaattcgccc gctactggga tgtggagctg cgtgagatcc ctatgcgccc
      661 cggtcagttg tttatggacc cgaaacgcat gattgaagcc tgcgacgaaa ataccatcgg
      721 cgtggtgccg actttcggcg tgacctacac cggtaactat gagttcccgc aaccgctgca
      781 cgatgcactg gataaattcc aggccgacac cggtatcgac atcgacatgc acatcgacgc
      841 cgccagcggt ggctttctgg caccgttcgt cgccccggat atcgtctggg acttccgcct
      901 gccgcgtgtg aaatcgatca gtgcttcagg ccataaattc ggtctggctc cgctgggctg
      961 cggctgggtt atctggcgtg acgaagaagc gctgccgcag gaactggtgt tcaacgttga
     1021 ctacctcggt ggtcagattg gtacttttgc catcaacttc tcccgcccgg cgggtcaggt
     1081 gattgcacag tactatgaat tcctgcgcct tggtcgtgaa ggctatacca aagtacagaa
     1141 tgcttcctac caggtcgctg cctatctggc ggatgagatc gccaaactgg ggccgtatga
     1201 gttcatctgt accggtcgcc cggacgaagg catcccggcg gtttgcttca aactgaaaga
     1261 tggtgaagat ccgggataca ccctgtacga cctctctgaa cgtctgcgtc tgcgcggctg
     1321 gcaggttccg gccttcactc tcggcggtga agccaccgac atcgtggtga tgcgcattat
     1381 gtgtcgtcgc ggcttcgaaa tggactttgc tgaactgttg ctggaagact acaaagcctc
     1441 cctgaaatat ctcagcgatc acccgaaact gcagggtatt gcccagcaga acagctttaa
     1501 acatacctga taacgtttaa cggtaacggt gtcccgaaac gaacccgttt cgggataatt
     1561 tccaaagtct gttcactggc attagcaacg gaaaatattg ttctgaatac gcttcagaac
     1621 aaaacaggtg cggttccgac aggaataccg ttttaggggg ataatatggc tacatcagta
     1681 cagacaggta aagctaagca gctcacatta ctcggattct ttgccataac ggcatcgatg
     1741 gtaatggctg tttatgaata ccctaccttc gcaacatcgg gcttttcatt agtcttcttc
     1801 ctgctattag gcgggatttt atggtttatt cccgtgggac tttgtgctgc ggaaatggcc
     1861 accgtcgacg gctgggaaga aggtggtgtc ttcgcctggg tatcaaatac tctgggtccg
     1921 agatggggat ttgcagcgat ctcatttggc tatctgcaaa tcgccattgg ttttattccg
     1981 atgctctatt tcgtgttagg ggcactctcc tacatcctga aatggccagc gctgaatgaa
     2041 gaccccatta ccaaaactat tgcagcactc atcattcttt gggcgctggc attaacgcag
     2101 tttggtggca cgaaatacac ggcgcgaatt gctaaagttg gcttcttcgc cggtatcctg
     2161 ttacctgcat ttattttgat cgcattagcg gctatttacc tgcactccgg tgcccccgtt
     2221 gctatcgaaa tggattcgaa gaccttcttc cctgacttct ctaaagtggg cacactggtt
     2281 gtatttgttg ccttcatttt gagttatatg ggcgtagaag cttccgcaac tcacgtcaat
     2341 gaaatgagca accccgggcg cgactatcca ctggctatgt tactgctgat ggtggcggca
     2401 atctgcttaa gctctgttgg cggtttgtct attgcgatgg tcattccggg taatgaaatc
     2461 aacctctccg caggggtaat gcaaaccttt accgttctga tgtcccatgt ggcaccggaa
     2521 attgagtgga cggttcgcgt gatctccgca ctgctgttgc tgggtgttct ggcggaaatc
     2581 gcctcctgga ttgttggtcc ttctcgcggg atgtatgtca cagcgcagaa aaacctgctg
     2641 ccagcggcat tcgctaaaat gaacaaaaat ggcgtaccgg taacgctggt catttcgcag
     2701 ctggtgatta cttctatcgc gttgatcatc ctcaccaata ccggtggcgg taacaacatg
     2761 tccttcctga tcgcactggc gctgacggtg gtgatttatc tgtgtgctta tttcatgctg
     2821 tttattggct acattgtgtt ggttcttaaa catcctgact taaaacgcac atttaatatc
     2881 cctggtggta aaggggtgaa actggtcgtg gcaattgtcg gtctgctgac ttcaattatg
     2941 gcgtttattg tttccttcct gccgccggat aacatccagg gtgattctac cgatatgtat
     3001 gttgaattac tggttgttag tttcctggtg gtacttgccc tgccctttat tctctatgct
     3061 gttcatgatc gtaaaggcaa agcgaatacc ggcgtcactc tggagccaat caacagtcag
     3121 aacgcaccaa aaggtcactt cttcctgcac ccgcgtgcac gttcaccaca ctatattgtg
     3181 atgaatgaca agaaacacta accatgtctg aatggttata agacaaggga gcgataattc
     3241 atcgctccct ttttcgtgct tgctgtcgta ttgacttaac cgggataagt acgagaatga
     3301 gcgcacatct gtttaccgga aaccagcaca tatggatatc tgctcccgaa acaagaaatt
     3361 aacgattaga agaccagcga tactggttgc attggcactt ttactgtgta gttgtaaaag
     3421 cacgcctcca gagtccatgg tgacaccacc agcaggttca aagccaccag ccacgacgca
     3481 acaatcgtca caaccgatgc gtggcatctg gctggccacg gtttctcgcc tcgactggcc
     3541 accggtttcc tcggttaaca ttagtaatcc caccagccga gcccgtgtac aacaacaggc
     3601 gatgatcgac aaactggatc atctccaacg tctcggcata aacacggtct ttttccaggt
     3661 caagccggac ggtaccgccc tgtggccatc gaaaattttg ccgtggtccg atcttatgac
     3721 cggtaagatt ggtgaaaatc cgggttacga tccgctgcaa ttcatgctcg acgaagccca
     3781 caagcgtggg atgaaagtac acgcctggtt taacccctat cgcgtatcgg ttaatacgaa
     3841 gcccggtact atcagggaac tgaatagcac tctgtctcaa caaccggcga gcgtctatgt
     3901 gcaacaccgt gactggatca gaacgtctgg cgatcgcttt gtcctcgacc cgggcatccc
     3961 tgaggttcag gactggatca catcaatagt cgctgaagtg gtttcccgct atccggtaga
     4021 tggcgtgcag tttgacgact atttctatac tgaatcaccg ggttcacggc taaatgataa
     4081 cgaaacgtac cgtaaatacg gaggcgcatt tgcgtcaaaa gcagactggc ggcgcaacaa
     4141 tactcagcag ttaattgcaa aggtatcaca caccattaaa agcattaagc cgggagtcga
     4201 atttggcgtt agcccggcag gcgtgtggcg taaccgatca cacgatccgc tcggttccga
     4261 tacccgaggc gcggcagcct atgacgaatc ctacgcagac acccgtcgat gggtggaaca
     4321 aggattgctg gattacattg ctccccaaat ttactggccg ttctcacgga gtgccgcgcg
     4381 ttatgacgtg ttggcaaaat ggtgggcgga tgtcgttaaa ccaaccagga cccgcctgta
     4441 tatcggtatc gccttctata aagtgggtga accttcaaag atagagccag actggatgat
     4501 taacggcggc gtaccggaac tgaaaaagca gctcgatctt aacgatgcgg taccagaaat
     4561 tagcggcacc atcttgttcc gtgaggacta tctgaataaa ccgcagactc aacaagcggt
     4621 cagctatctg caaagtcgtt ggggcagtta aactaccatg cttactttgt aacaagccgg
     4681 tgctttaccc accggcttgt tacaccttgt gaaatatccc actagtaatc atgcttacat
     4741 aagtcaaatt aaccacgcaa ttaaccacac ttagttaaac agctataaat ccctaataaa
     4801 aatgtatgaa tatgaaaaac catacgatat tcgctgtcac cgaaaaataa ttacgatggg
     4861 ctatactctt ctgaagaaca gtagcgtaga ttgaaactgt cttaagtcgc ttatttcttt
     4921 tcagtatatc ttcatatttc aggagaatat ggtgcagtta gtcgatctcg cacgttgcgt
     4981 ttcgttttga ttccgtctga tttcccagcc ttataagggt gtgaattatg gagatgtatt
     5041 ttaaaagaat gaaagatgag tggaccggac ttgtcgaaca ggcagatcct ctcattcgtg
     5101 ctaaagccgc agaaattgcc cttgcgcatg ctcattatct gagtatcgag ttttatcgaa
     5161 ttgtccgcat cgacccgcat gccgaagaat tcctgagtaa tgaacaagtt gagcggcagt
     5221 tgaaaagtgc gatggaacgc tggattatta acgtgctttc tgcccaggtt gacgatgtcg
     5281 aaaggctaat acaaatccag cataccgtcg cggaagtgca tgcccgcata ggaattccgg
     5341 tagaaattgt cgagatgggg tttcgggtgc tgaaaaaaat cctctatccg gtaatcttct
     5401 cttcggatta ttccgccgca gaaaaacttc aggtctacca tttctcgatt aacagtattg
     5461 atatcgcgat ggaagtgatg acccgcgcgt ttacctttag tgacagtagt gcctcaaagg
     5521 aagatgaaaa ctatcgtatc ttctcgttac tggaaaacgc cgaagaagaa aaagaacggc
     5581 aaatagcctc aatactttca tgggaaatag atattatcta taaagtcctg ctggattctg
     5641 atttaggcag cagtttgcct ttaagccagg ctgattttgg cctgtggttt aaccataaag
     5701 gtcgacatta ttttagcggc attgccgaag tgggccatat ctcccgtctg attcaggatt
     5761 tcgacggtat tttcaatcaa accatgcgta acaccaggat tttgaataac agaagtttgc
     5821 gggtgaaatt tctattacag ataagaaata ccgtatcgca gattattacc ttgctgcgtg
     5881 aattgtttga agaagtatcg cgccacgaag tcggtatgga tgtactgacg aaattactta
     5941 accgccgttt cctgccgact atcttcaaac gcgaaattgc ccatgccaac cggaccggta
     6001 caccgctgtc agtgctgatt attgacgttg ataaattcaa agagatcaac gatacgtggg
     6061 gccataacac tggcgatgaa attctgcgta aagtctcatt cctctcgcag aagagattgg
     6121 tgaaatcgaa aatattgggc gctgggtcat cgcggaagct tgccgtcagt tagcagaatg
     6181 gcgtagccag aatattcata tcccggcgtt atccgtgaac ttgtcggcgc tgcactttcg
     6241 cagtaatcag ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta ttgacggcca
     6301 ccagctgacg gtggaaatca cggaaagcat gatgatggaa cacgataccg aaatctttaa
     6361 gcgcattcag atcctgcgtg atatgggcgt gggtttatcg gtagatgatt ttggcacggg
     6421 cttttccgga ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa ttgacaaaag
     6481 ctttgtcgat cgttgtctga cggaaaaacg catcctggcc ttacttgaag ccattaccag
     6541 cattgggcaa agcctcaatt taaccgtcgt ggcggaaggg gtcgaaacca aagagcaatt
     6601 tgagatgcta cgtaagatcc actgtcgcgt tattcaggga tatttctttt cccgccccct
     6661 tcccgccgaa gaaattccag gctggatgag cagcgtgtta ccgctgaaaa tctgacaaat
     6721 tcctctcgcc cgcactcgcg ggttttcctt taaggtgaca ctgtcactta aatactgtga
     6781 tttcagctac gattccggga gattccttcc ttaactttcc ctcttgcacc aaaactgcac
     6841 tacgctgcac aaatgttgat caaaagttaa aaaatataac tttaagccag cattctggcg
     6901 cttatcccgg catggcatga gatctgcata agcggaaagc gcagcaattt ttgtcttata
     6961 caggaaccgt ttatgtcgga taccaccgaa ctggttgatt tagccgtgat cttccctgat
     7021 ctggagatcg aattgaaata tgcctgcgtt gataacatca caggtaaagc tatttatcag
     7081 caagcgcgtt gtctgctaca taaagatgcg attaccgcgc tggcgaaaag tatcagtatc
     7141 gcccagctgt caggcttaca actggtgatt tacgatgcgt atcgcccaca acaagcacag
     7201 gcgatgttgt ggcaagcctg cccagacccg caatatgttg ttgatgtgac ggtcggttct
     7261 aatcacagcc gtggcacggc gctcgacctg acgcttcgtg atgacgtgga ggtggtgaat
     7321 gatgctaagc gaggaaacgt ccgccgtacg cccacaaaaa caaacgcgat ttaacggtgc
     7381 aaaactggta tggatgctga aaggcagtcc gctcaccgtg accggcgcag tcatcattgt
     7441 attaatgcga ctgatgatga ttttttcacc gtggctggcg acgcatgatc ccaacgccat
     7501 tgatttaacc gcccgccttt tgccgccttc tgcggcgcac tggtttggca ccgatgaagt
     7561 gggacgcgat ctgtttagcc gcgtactggt cggcagtcag caatcaattc tcgccggatt
     7621 agtggtggtc gccattgcgg gtatgattgg ttcgctactc ggatgtctat ccggtgtgct
     7681 tggcggacgc gcagacgcca tcatcatgcg catcatggac attatgctgt cgattccttc
     7741 gttggtactg acaatggcac tggctgccgc tctcgggccg agtttattta acgccatgct
     7801 ggcgattgct attgtgcgaa ttccctttta tgtgcgcttg gcgcgggggc aagcattagt
     7861 tgtacgccag tatacctatg ttcaggcggc gaaaaccttt ggcgcgtctc gttggcatct
     7921 gatcaactgg catattttac gtaactccct accgccgctg atcgtgcagg catcgctgga
     7981 tatcggtagc gcgattttaa tggccgccac gttgggattt attggcctgg gtgctcaaca
     8041 accgagtgct gaatggggcg cgatggtagc gaatggtcgc aactatgtac tcgatcagtg
     8101 gtggtattgc gcatttccgg gggcagcgat tttgcttacc tccgtcgggt ttaatctctt
     8161 tggtgatggt attcgcgatc tgcttgaccc gaaagcagga ggaaagcagt catgacccaa
     8221 cccgttctgg acattcaaca gctgcatttg agtttccccg gttttaacgg tgatgttcac
     8281 gcgctcaaca atgtgtcctt gcagattaac cgcggcgaaa ttgtcggtct ggtgggagaa
     8341 tccggctcag gtaaatcagt caccgcaatg ctgattatgc gtctgctacc gacgggcagt
     8401 tattgcgtac atcgggggca gatttcactg ctgggagaag atgttcttaa cgctcgggaa
     8461 aaacagcttc gtcagtggcg cggcgcacga gtggcgatga tctttcagga accgatgacc
     8521 gcccttaatc cgacacgtcg aataggtctt cagatgatgg acgtaatccg ccatcatcaa
     8581 ccaataagtc gtcgggaagc cagagctaaa gcgattgcac tgctggaaga gatgcaaatc
     8641 ccggatgccg tggaagttat gtcgcgctat ccgtttgagc tttcaggtgg tatgcgccag
     8701 cgggtaatga ttgcgccggc attctcctgc gagccgcaat tgattattgc cggcgaaccg
     8761 acgacggcgc tggacgtcac agtacagttg caggtactgc gtttgcttaa acataaagca
     8821 cgcgccagtg gaactgcggt actgttcatc agccatgata tggccgtggt gtcgcaactg
     8881 tgcgatagcg tttacgtgat gtatgccgga agcgtaattg aaagcggcgg gacggcagac
     8941 gttatccatc atccccggca tccgtatacc attggtttgc tgcaatgcgc accagaacat
     9001 ggagtaccac gccagccatt acccgccatt ccagggacgg taccaaacct cacccatttg
     9061 cctgacggct gcgcttttcg cgatcgttgc tatgcggcag gtgcacagtg tgcgctgaca
     9121 gcgtgtggtg acaacaacca gcactgcacc tgttggtatc cgcagcagga ggtcataagt
     9181 gtctgacacg ttattaacgt tacgcgacgt ccatatcaat tttccggccc gtaaaaactg
     9241 gcttggcaaa tctacggaac atgttcatgc cattaatggt attgatttac agatccgccg
     9301 tggtgaaacc ttagggatcg tcggcgagtc aggctgcggc aaaagcaccc tcgcacagct
     9361 tttaatgggc atgctgcaac cgagccacgg gcagtacatc cgttcaggct cacaacgcat
     9421 tatgcagatg gtgtttcagg acccgctctc ttcgcttaat ccgcgcttac cggtgtggcg
     9481 catcatcaca gaatcgctct ggatagctaa gcgtagtagt gaacaacagc ggcgagcgtt
     9541 ggcagaggag ttggctgtgc aggtgggtat tcgtccggag tatctcgacc gcctgcctca
     9601 tgcgttctcc ggcgggcagc ggcaacgcat cgccattgcc agagcactct cttcgcagcc
     9661 tgacgtgatt gtgcttgatg agccaacctc ggcgctggat atctccgtgc aggcgcagat
     9721 cctcaatttg ctggtaacgc tacaggaaaa tcacgggctg acttatgtgc tgatttcaca
     9781 caatgtctcg gtgatacgtc atatgagcga tcgggtggcg gtgatgtatc tcgggcagat
     9841 tgtagaactg ggcgacgcgc aacaggtgtt gactgcttcg gcacatccat acacccgatt
     9901 attgctggat tccctccccg ccattgataa accgctggag gaagaatgag cattacgtaa
     9961 aacggatctg ccgggaaacc gcacgttgcc gcaaggctgt tttttctacg aacgttgccc
    10021 tctggcaacc cacggatgtg aagtccggca atcattagca ataagggagg acggacgtga
    10081 cctccggtgc tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg
    10141 acgcgtcatc cggcaatgct ttacgatttc aactggtaat ccagcgtaat ttccgctttc
    10201 agtacctgag agaccgggca tcctgctttt gctttctgga tgatgccgtc aaaggtagag
    10261 gcatcaatac ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat cgcaaaaccg
    10321 gcatccactt tatccagcga cacatcggcg gtggtatcaa ttgatgttgg cgtgaatccc
    10381 gcttccccca gcattaatga aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc
    10441 agttcttcag ggttggttcc tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt
    10501 tggttcagca cgccgctctc ggtggatact gttcccttcc cgcgtttgat atcgccttcc
    10561 cagtgtgcct gacctttctt atggattgtc attgttgctc tcctgtgggc ttaaaaagga
    10621 aattatagac tacgtgagaa acagcacgag gataagcagg atattccgaa taaaatacgg
    10681 cgaaaatatt acttctgcac ttaaattaaa ttcccacaat agataaccgg gaatatatat
    10741 ctcaag
//
LOCUS       DAZXUL010000133        10465 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZXUL010000133 DAZXUL010000000
VERSION     DAZXUL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.133"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="ACH3L1_003522"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="ACH3L1_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFT4078123.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="ACH3L1_003523"
     CDS             214..402
                     /locus_tag="ACH3L1_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFT4078124.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="ACH3L1_003524"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="ACH3L1_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFT4078125.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="ACH3L1_003525"
     CDS             597..770
                     /locus_tag="ACH3L1_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFT4078126.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="ACH3L1_003526"
     CDS             complement(819..1892)
                     /locus_tag="ACH3L1_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFT4078127.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4705)
                     /gene="torS"
                     /locus_tag="ACH3L1_003527"
                     /pseudo
     CDS             complement(1994..4705)
                     /gene="torS"
                     /locus_tag="ACH3L1_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="ACH3L1_003528"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="ACH3L1_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFT4078128.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="ACH3L1_003529"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="ACH3L1_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFT4078129.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="ACH3L1_003530"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="ACH3L1_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFT4078130.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="ACH3L1_003531"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="ACH3L1_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10465
                     /gene="torD"
                     /locus_tag="ACH3L1_003532"
     CDS             10277..>10465
                     /gene="torD"
                     /locus_tag="ACH3L1_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFT4078131.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2524 a         2685 c         2789 g         2467 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tgactgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattccagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccacg
//
LOCUS       DAZXUL010000134        10408 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZXUL010000134 DAZXUL010000000
VERSION     DAZXUL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.134"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1302)
                     /locus_tag="ACH3L1_003533"
     CDS             complement(<1..1302)
                     /locus_tag="ACH3L1_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078132.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLL"
     gene            complement(1577..2941)
                     /gene="tdcG"
                     /locus_tag="ACH3L1_003534"
     CDS             complement(1577..2941)
                     /gene="tdcG"
                     /locus_tag="ACH3L1_003534"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFT4078133.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(3013..3402)
                     /locus_tag="ACH3L1_003535"
     CDS             complement(3013..3402)
                     /locus_tag="ACH3L1_003535"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFT4078134.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(3416..5710)
                     /gene="tdcE"
                     /locus_tag="ACH3L1_003536"
     CDS             complement(3416..5710)
                     /gene="tdcE"
                     /locus_tag="ACH3L1_003536"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFT4078135.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(5744..6952)
                     /gene="tdcD"
                     /locus_tag="ACH3L1_003537"
     CDS             complement(5744..6952)
                     /gene="tdcD"
                     /locus_tag="ACH3L1_003537"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFT4078136.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(6978..8309)
                     /gene="tdcC"
                     /locus_tag="ACH3L1_003538"
     CDS             complement(6978..8309)
                     /gene="tdcC"
                     /locus_tag="ACH3L1_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFT4078137.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(8331..9320)
                     /gene="tdcB"
                     /locus_tag="ACH3L1_003539"
     CDS             complement(8331..9320)
                     /gene="tdcB"
                     /locus_tag="ACH3L1_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFT4078138.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(9419..10357)
                     /gene="tdcA"
                     /locus_tag="ACH3L1_003540"
     CDS             complement(9419..10357)
                     /gene="tdcA"
                     /locus_tag="ACH3L1_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFT4078139.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         2769 a         2733 c         2398 g         2508 t
ORIGIN      
        1 aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt tgtgcaatgc
       61 cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga aaatcgggct
      121 acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc aggccagcaa
      181 aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct cggcaggcat
      241 cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac
      301 gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca ctttcaccca
      361 cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc
      421 gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg cgtagaaaaa
      481 gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt tatgccgcgc
      541 cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta acgtctggat
      601 aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca ccagcagccc
      661 cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg acacgcccag
      721 cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga ataacaattt
      781 ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat aaaagggact
      841 gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac tatcgttggt
      901 gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga gcgcacctaa
      961 caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat ctttacattc
     1021 tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg ccgggtaacc
     1081 aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca ccgggagaaa
     1141 aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg tgtcagtact
     1201 gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac ccgccggggt
     1261 cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc gctcaatatt
     1321 ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc tacgtttttg
     1381 caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat cttcacaatc
     1441 aagactttaa aataacggag atggataaga attttctact aaattaatca caagagaata
     1501 ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca cccaaggatg
     1561 aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg cgtgatgttt
     1621 cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc actttatcga
     1681 gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt actgctttca
     1741 cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca accggatcgc
     1801 aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc tgcgccggac
     1861 tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg ccaatctccc
     1921 cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc gcgccaatag
     1981 cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga cggaacttat
     2041 cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt gccgttacca
     2101 cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg atccagtcga
     2161 tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga cgcagcgcta
     2221 cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt
     2281 caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt tccgctttgc
     2341 tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg ttgtaatcac
     2401 acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt
     2461 gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag taataggttt
     2521 tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc tcatggcgag
     2581 gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca atatgcgcac
     2641 cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca gggatctcat
     2701 caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg acatccgtgg
     2761 catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata tggctcgtcg
     2821 cggttaataa gccgctactt tccagccgat caataaaact ttttccggca ttcattggcc
     2881 ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat gcactaatca
     2941 tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg cgaccgtccc
     3001 gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct tcacatcttt
     3061 cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa
     3121 gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga tgaacacggt
     3181 catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt tgacgttttc
     3241 gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct gcgggcaaac
     3301 cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa cataagggcc
     3361 gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc cggcgtcaga
     3421 gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc agtgcgttga
     3481 agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc gggtgctcga
     3541 tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga ccgccttcga
     3601 catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt ttacgtactg
     3661 gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc ccatctttgg
     3721 cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct ttgcggtcac
     3781 gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg
     3841 tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc gactgggtag
     3901 ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt tcaaccaggt
     3961 cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga
     4021 tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg gcatatttga
     4081 tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc atagtgcgat
     4141 agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg tcgtgcatgt
     4201 agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg aagtgatcga
     4261 ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct gttttcggcc
     4321 cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc agcgttttcg
     4381 ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt gggctgacgc
     4441 agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg tcattttcat
     4501 actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag gcaatcggta
     4561 attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata gtgtgcaggg
     4621 tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc cccatcccgc
     4681 cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat tccggtgtac
     4741 gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc tgtgcctgct
     4801 gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg aacgatgccg
     4861 tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga taagcgaagt
     4921 agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca aagccatatt
     4981 tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc tcctcacgca
     5041 gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga tcggcaaatt
     5101 gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg cgatagtcac
     5161 cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca gatttacggc
     5221 agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt ttacgcagat
     5281 cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg aatgaacttt
     5341 taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca tccgtttgca
     5401 ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg gtaattgtgg
     5461 tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata cggatgcctt
     5521 ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga aaagattcat
     5581 cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt tcgcttttcc
     5641 agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg gtatcaatat
     5701 ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca aattctgcgg
     5761 gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct tcgttagtcg
     5821 gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg ccaaaagagt
     5881 tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa tgttccatga
     5941 ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata attccatcca
     6001 gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga acaaaggttt
     6061 taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga acacgtaaat
     6121 ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg cgttccaggt
     6181 cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag tcgacatcgc
     6241 cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag gtgtcaacac
     6301 tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc acaaccaggc
     6361 cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca tagcggtgcg
     6421 acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc cacggcaggc
     6481 cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat accgccacct
     6541 gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt gcgtaattat
     6601 gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg gtaataatgg
     6661 cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag gccacgctgt
     6721 catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct tcgtagctgt
     6781 ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt tccgagttaa
     6841 taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt acggaaaact
     6901 taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc atctcttttc
     6961 tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt cagcagacca
     7021 atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga cggcgcttta
     7081 cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc gcccatggct
     7141 tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga gcccatgatg
     7201 aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc acctttataa
     7261 ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg accgaagaaa
     7321 gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt aatcgcgaac
     7381 gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag cactggaata
     7441 ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca gctaaaggca
     7501 aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg ggaacattta
     7561 cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga aaccacgaag
     7621 gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc cagccagaca
     7681 gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac ctggtcgata
     7741 actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct ggcgataaac
     7801 ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca gatgacgaaa
     7861 gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc aaatccgagc
     7921 tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat ccacagcagt
     7981 gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt accaaaatgc
     8041 tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca cagacgcgcc
     8101 agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag aagaatcggg
     8161 atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc gattgccgta
     8221 ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg ttttgtctgg
     8281 ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa ttaagcgtca
     8341 acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga aataatactg
     8401 acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc agcacatgcc
     8461 agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat catactgttt
     8521 ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac gatttcgtaa
     8581 gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt agttcggtgc
     8641 gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc tgactgtacg
     8701 ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc agcaattaaa
     8761 ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc cataatttcc
     8821 agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg gataaaaata
     8881 cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa gttatcacca
     8941 tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt tggcgcacct
     9001 tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga aaccccttgc
     9061 gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc ggtcagtgaa
     9121 cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat attttcaaac
     9181 ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg catgcctgtt
     9241 ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat agcaaccggc
     9301 agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg taggtaaccg
     9361 taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa acaaacaact
     9421 aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac tctttggcta
     9481 attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat accgcggcat
     9541 attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta gaaccaaaag
     9601 gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca agattataaa
     9661 ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt ctttgtaacg
     9721 tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac tgttcgttct
     9781 tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct accagcacaa
     9841 actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct gcacttaacg
     9901 taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg
     9961 cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg atcatccctg
    10021 acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc accgcttcag
    10081 aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg gattcggaac
    10141 gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc cgcaccacta
    10201 attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc ggttgagtta
    10261 accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc tgaaagacta
    10321 ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt aattacctca
    10381 ttgacggcat gaagtgtatc aaaatgaa
//
LOCUS       DAZXUL010000135        10116 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZXUL010000135 DAZXUL010000000
VERSION     DAZXUL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10116
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.135"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            134..223
                     /locus_tag="ACH3L1_003541"
                     /pseudo
     CDS             134..223
                     /locus_tag="ACH3L1_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            525..1520
                     /gene="pgl"
                     /locus_tag="ACH3L1_003542"
     CDS             525..1520
                     /gene="pgl"
                     /locus_tag="ACH3L1_003542"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFT4078140.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1561..2514)
                     /locus_tag="ACH3L1_003543"
                     /pseudo
     CDS             complement(1561..2514)
                     /locus_tag="ACH3L1_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2698..3750
                     /locus_tag="ACH3L1_003544"
     CDS             2698..3750
                     /locus_tag="ACH3L1_003544"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFT4078141.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3826..5259
                     /locus_tag="ACH3L1_003545"
     CDS             3826..5259
                     /locus_tag="ACH3L1_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFT4078142.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5442..7622
                     /locus_tag="ACH3L1_003546"
     CDS             5442..7622
                     /locus_tag="ACH3L1_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFT4078143.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7763..9046)
                     /locus_tag="ACH3L1_003547"
     CDS             complement(7763..9046)
                     /locus_tag="ACH3L1_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFT4078144.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9181..>10116)
                     /locus_tag="ACH3L1_003548"
     CDS             complement(9181..>10116)
                     /locus_tag="ACH3L1_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4078145.1"
                     /translation="TEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILAS
                     RGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTL
                     SDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMEL
                     AVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCK
                     EILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQ
                     ISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2455 a         2360 c         2553 g         2748 t
ORIGIN      
        1 tcaatgtact gtttttttct ttaaaaaaac agtacattct attttataat gactgtgtat
       61 tcccatggaa ggagatcacc caattctccc ccccgacttt tgacgcagtg tcttgttaac
      121 agggggattg accatgctta ttggctatgt acgcgtatca acaaatgacc agaataccga
      181 tctacaacgt aatgcgttta tttgtgccgg aagtgagttg attgttgcgc gaatgggaag
      241 ttggccagaa gttgttatgg catagtgttt acggtcgttg atacaggtaa acaaaagcgg
      301 ggagagtcat actccctgaa tagtcgcgaa aatatctgtg gcattgtcaa attcgtgtcg
      361 gtgactaata ttttagtaat atgccattcg aaatggagtg ctaacttatt gatttaattg
      421 atgtgcgata ttgtatttta tcgtgttttg ttgtctgttt tttatccagt aagtgttgtt
      481 atatgtatga aagataaggg atttatcatt tttgaggtgt gttcatgaag caaacagttt
      541 atatcgccag ccctgagagc cagcaaattc acgtctggaa cctgaatcat gaaggcgcac
      601 tgacgctgac acaggttgtc gatgtgccgg ggcaggtgca gccgatggtg gtcagcccgg
      661 acaaacgtta tctctatgtt ggtgttcgcc ctgagtttcg cgtcctggcg tatcgtattg
      721 ccccggacga tggcgcactg acctttgccg cagagtctgt gctgccgggt agtccgacgc
      781 atatttccac cgatcaccag gggcagtttg tctttgtagg ttcttacaat gcgggtaacg
      841 tgagcgtaac gcgtctggaa gatggcctgc cagtgggcgt cgtcgatgta gtcgaggggc
      901 tggacggttg ccattccgcc aatatctcac cggacaaccg tacgctgtgg gttccggcat
      961 taaagcagga tcgcatttgc ctgtttacgg tcagcgatga tggtcatctg gtggcgcagg
     1021 accctgcgga agtgactacc gttgaagggg ccggcccgcg tcatatggta ttccatccaa
     1081 acgaacaata tgcgtattgc gtcaatgagt taaacagctc agtggatgtc tgggaactga
     1141 aagatccgca cggcaatatc gaatgtgtcc agacgctgga tatgatgccg gaaaacttct
     1201 ccgacacccg ttgggcggct gatattcata tcaccccgga tggtcgccat ttatacgcct
     1261 gcgaccgtac cgccagtctg attaccgttt tcagcgtttc ggaagatggc agcgtgttga
     1321 gtaaagaagg cttccagcca acggaaaccc agccgcgcgg cttcaatgtt gattacagcg
     1381 gcaagtatct gattgccgct gggcaaaaat ctcaccacat ctcggtatac gaaattgttg
     1441 gcgagcaggg gctactgcat gaaaaaggcc gctatgcggt cgggcaggga ccaatgtggg
     1501 tggtggttaa cgcacactaa ccgctgattt aaccggcgca gtctctcctg cgccggtgta
     1561 ttaacttatc tcctgtaacg catgtctctg gcgttcgacg atattggtcc acaaattgtc
     1621 tttatcgtca gtccacaaat taatcagcaa ggaaaaaaag cgttcagctg ccggagaaag
     1681 cacggcatct ttgcggcgaa taatccccaa tgtccgacgt ataacgggtt ccaccagtgg
     1741 gataccaata atcgaagaat agggcgcatg gggcattgcc agaccaggaa gcgccgaaat
     1801 ccccagtcct gcctccacta gtcctaatga cgtcgaaaga tgacgcactt cgtaaaacca
     1861 gtccagcttc cagggcttgt cggctagctg ttgttctatc aacaatcggt tgccgctgga
     1921 ggaacgtacg ccaatcattt tgtaacccac taactcctgc cattctacaa gctacttttt
     1981 ggccaatgga tggtcacgcc gacaggccag cacgaatggc tcgttgacca gtggggtaaa
     2041 atcaatggat gaatttgtga cgttgttcat gtttatgcca aagtctgatt cgttacacag
     2101 caccgattcc atgcaattat tcgtgccttg ttccagaatc cgcactttaa tattgggata
     2161 cagctcatta aatttaccga ttgccagcgg taaaaaataa aatactgcgg tcggaataca
     2221 cgctaatgtc accataccac gatgataagc attcatatca cgaatattaa aaagcgtttc
     2281 atcgaatttc tttattaatt cccgcgcctc cgggagcaac ctttttcctg cttttgttaa
     2341 cgtaaccttg cgagttgtac gctcaaatag ttgaatatgt aaatcttcct ccatcttttt
     2401 tattcttcgc gttaatgcag gttgcgtaat attgagtaat ttagcggcat tgttaaatga
     2461 actggactct gccagtatga caaatgcctt catacttgat aattcatgct tcattattac
     2521 tccggaaaat ggaagcgacg attttggggg gctggtcgtt aaaaatttta actgcattta
     2581 gccaacttaa attaatgaaa aaatgttatt aatcgttgag ctaaagtcat tagagatgct
     2641 ttgtccttaa tgtaaccata tcgcaataag ttatgttttt aaattgaggg cattattatg
     2701 aaaaaaatac cctgcgtgat gatgcgaggt ggaacctcga ggggcgcgtt cctgttagcg
     2761 gaacatttac ccgaagatca aacgcagcgc gataaaatat tgatggcaat tatgggttcc
     2821 ggtaacgatc tggaaattga cggtattggc ggcggtaatc cactgaccag taaagtcgcc
     2881 attattagcc gttccagcga tccgcgtgct gatgtcgatt atctgtttgc tcaagttatc
     2941 gtccatgagc aacgtgtcga taccacgcct aactgcggca atatgctgtc tggcgtaggg
     3001 gcattcgcca ttgaaaatgg tttgattgca gcgacttcgc cagttacccg tgtacgtatc
     3061 cgcaacgtca atacgggtac gttcatcgaa gctgatgtgc aaacgccaaa tggtgttgtc
     3121 gagtacgagg gtagcgccag aattgacggc gtaccgggta ctgccgcacc ggttgcgctc
     3181 actttcctga atgccgctgg aacgaaaact ggaaaagttt tcccgactga taatcagatt
     3241 gattattttg acgatgtccc ggtgacctgt atcgatatgg cgatgccagt cgtcattatt
     3301 ccggctgaat atctgggaaa aacaggttat gaattaccgg cggaactgga tgccgacaaa
     3361 gcattattag cccgcattga atctatccgg ctacaagcgg gtaaagcaat gggcttaggt
     3421 gatgtcagta atatggttat ccctaaacct gtgcttattt ctccagcgca gaaaggcggg
     3481 gcaattaatg tgcgttattt tatgccgcat tcttgccatc gcgcgctggc gataaccggt
     3541 gctattgcta tttccagtag ttgtgcattg gaaggcaccg tcacccgaca aatcgtccct
     3601 tctgtaggat atggcaatat caatattgaa caccccagtg gtgcgctcga cgttcattta
     3661 agtaatgaag gtcaggatgc cacgacgtta cgcgcatctg ttattcggac aaccagaaaa
     3721 atattttccg gtgaagttta tcttccctga aaaaattcgt tgtcaggata aggacaatca
     3781 ataaaggact tctgtatgag tcatacagaa agaacaggat tttaaatgaa taagaaatcg
     3841 ttatggaagc taattctgat attagcgatc ccatgtatta ttggttttat gccagctccg
     3901 gcaggattaa gcgaactggc gtgggtgctt tttggtattt acctggcggc cattgtgggt
     3961 ctggttatca agcctttccc ggaacctgtc gtactgttaa ttgccgttgc tgcctcaatg
     4021 gtggtggtcg gtaacttatc cgacggtgcg tttaagacca ccgccgtatt aagcggttac
     4081 tcttcaggta ctacctggct ggtgttctcg gcgtttacct taagcgccgc atttgtgacc
     4141 accggtttag gtaaacttat tgcctatctg ctgattggta aaatcggtaa caccatgctg
     4201 ggtctgggtt acgttacggt attcctcgat ctggtactgg ctccggcaac accgtctaac
     4261 accgcgcgtg cgggcggcat tgtgttaccg atcatcaaca gcgtggcggt ggctttgggg
     4321 tccgaaccgg aaaaaagtcc gcgtcgtgtc ggacattacc tgatgatgtc catttacatg
     4381 gtcaccaaaa ccaccagcta tatgttcttt accgcaatgg cggggaacat tctggcgctg
     4441 aaaatgatca acgacattct gcacctgcaa attagctggg gtggatgggc gctggccgcc
     4501 ggattgccgg gcatcattat gctgctggtc accccgctgg taatttacac catgtatcca
     4561 ccagaaatta aaaaggtgga taacaaaacc atcgctaaag cgggccttgc cgaactggga
     4621 ccgatgaaaa tccgcgaaaa aatgctgctc ggtgtctttg tgctggcgct actgggctgg
     4681 attttcagta agtctctggg ggttgatgaa tccactgtgg caatcgttgt tatggcgacc
     4741 atgctgctgc tgggtatcgt tacctgggaa gacgtggtta aaaataaagg cggctggaat
     4801 accttaatct ggtacggcgg tattatcggc ttaagctcct tattatcgaa agttaaattt
     4861 ttcgaatggt tagctgaagt ctttaaaaat aacctggcat ttgatggtca cggtaacgtt
     4921 gctttcttcg ttattatttt cctcagcatc atcgtgcgtt atttcttcgc ttccggtagt
     4981 gcctatatcg ttgctatgtt acctgtattt gccatgctgg cgaacgtctc cggcgcaccg
     5041 ttaatgttaa ccgcgctggc actgttgttc tccaactcct atggcggcat ggttactcac
     5101 tatggcggcg cggcaggtcc ggtcatcttt ggcgtgggtt acaacgatat taaatcctgg
     5161 tggttggtcg gtgcggtact gacgatatta accttcttgg tgcatatcac cctcggcgtg
     5221 tggtggtgga atatgctgat cggctggaac atgctgtaaa tatactcgtc atacttcaag
     5281 ttgcatgtgc tgcgtctgcg ttcgctcacc ccagtcactt acttatgtaa gctcctgggg
     5341 attcactcac ttgtcgcctt cctgcaactc gaattattta gagtatatcc atttattatc
     5401 tttctgcgca cttcacggtg cgcagatatc tggagcattt gatgatcaag ttatctgaaa
     5461 aaggcgtgtt tctcgccagt aataacgaaa taattgccga agaacatttc accggcgaaa
     5521 ttaaaaaaga agaagcaaaa aaaggcacta ttgcctggtc tattctctct tctcataata
     5581 cgtccggaaa tatggataaa cttaaaatta agtttgattc attagcctct cacgatatta
     5641 cctttgttgg tattgtacag accgctaaag cgtccggaat ggaacgtttc ccgctgccgt
     5701 atgtgcttac caactgccat aactcactct gcgccgtcgg cggcaccatt aacggtgatg
     5761 accatgtttt tggtttatcg gcagctcagc gttatggcgg tatttttgtg cctccgcata
     5821 ttgcggtcat ccatcaatat atgcgtgaga tgatggctgg cgggggcaaa atgatcctcg
     5881 ggtcagacag ccacacccgt tacggtgcat tagggacaat ggcagtcggt gagggtggcg
     5941 gtgagttggt aaaacagctg cttaatgaca cctgggatat cgactatccg ggcgtggttg
     6001 cggtgcatct gaccggaaaa cctgcgccgt atgtggggcc acaggatgtg gcgctggcta
     6061 tcattggcgc ggtgttcaaa aacggttacg tcaaaaacaa agtcatggag ttcgttggac
     6121 cgggcgttag cgcgctctct accgatttcc gtaacagcgt tgacgtgatg accacggaaa
     6181 ccacctgttt aagctccgtc tggcaaactg atgaagaagt gcataactgg ctggcgctgc
     6241 acggtcgcgg tcaggattac tgccagctta accctcaacc gatggcgtac tacgatggct
     6301 gcatcagcgt tgatttaagc gccatcaaac caatgattgc gctgccgttc cacccgagca
     6361 acgtgtatga aatcgacaca ctgaaccaga acctgaccga cattctgcgt gagattgaaa
     6421 ttgagtccga acgcgtggcg cacggtaaag ccaaactctc gctgctggat aaagtggaaa
     6481 atggtcgcct gaaagtgcag caggggatta tcgcgggctg ttctggcggt aactacgaaa
     6541 acgtcatcgc ggcggcgaat gcactgcgcg gtcaatcctg tggcaatgac accttctcgc
     6601 tggcggttta cccgtcatca cagccggtgt ttatggatct cgccaaaaaa ggtgtggtag
     6661 cagatttgat tggcgcaggc gcaatcatca gaaccgcatt ctgtggccca tgctttggcg
     6721 cgggcgatac gccaatcaac aacggtttga gtattcgcca caccacgcgt aacttcccga
     6781 accgcgaagg ctctaagcca gccaatgggc agatgtcagc ggtggcgttg atggacgctc
     6841 gttctatcgc tgcgactgcg gcaaacggtg gctatttaac ctctgccagc gaacttgatt
     6901 gctgggacaa cgtgccggag tacgccttcg atgtaacgcc gtataaaaac cgtgtttatc
     6961 agggctttgt gaaaggggca actcagcaac cgctgattta cggaccgaac attaaagact
     7021 ggccggaatt gggtgcgctg actgacaata tcgtcctgaa agtgtgctcg aagatcctcg
     7081 acgaagtcac caccaccgac gaactgattc cttccggtga aacctcttct tatcgttcaa
     7141 atccgattgg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc
     7201 agattgctgg tcaggagcat attgatccgc tgcaaactga aattggtagc atggtctatg
     7261 cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg
     7321 gcggtctggc gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca
     7381 actgggggat gttaccgctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca
     7441 tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg
     7501 tgatccatga agatgcgccg gtaacggaaa ttacgcttta catggaaagc ctgacggctg
     7561 aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt
     7621 aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgctggcg
     7681 catcttatcc gacctacaaa tcgcatcgaa ccgtaggccg gataaggcgt ttacgccgca
     7741 tccggcaaat agttaatagc tcttacttct tctcctctgc aaccactttg ctgcccacgc
     7801 cgcggttatt gtattcccac atgcggttgt agttagtgtc attcagattg cgctgtattt
     7861 cgtcgttatc atctacgctg ccggtattac ccgcaaacgg acgattagag atcaccgcat
     7921 cggcccacgg tttagccgtg ttaaaacctt cgttgatggc gctatcacgg atcaccacct
     7981 gaccgttggt attagcatca acatccagcg agcggcccag ttgcgccacg ccatcaccgg
     8041 aagcattgaa acggctgttt acggcgagga aaccgtaata gatgttagac agcgtagccg
     8101 gtgcaaacac atatgcttct tgctgggtac gtgagttcac cacgcggaat tcggtgttat
     8161 cgaacaccac tgcgccgcga ccagaaacga tatccacatc cccttcaata tagctgttgg
     8221 tcaccagcgt acgcggctga cggttggttt ccagacggtt ctgcacaccg ctgttggtga
     8281 caaagaaggt gttctgacga ccgagaatgt taacgttgtt aatctgtact tggtcaccgt
     8341 cagtacgcag tgctaccgcc ggatggttac ctgcatctac gctatcgccc agcgtgtttt
     8401 cgatggtcag attttgcagt tgcagtccat tgttttgtga ccagaagacc gcagaacaca
     8461 gaacaccgat actgtcgctg cgtttgctct ggcagctatc gtacatatac cacgctggtt
     8521 tacctggcat atatttgccg cgcgggttga cgtcgtgacg ccagtcggca gggctcatgc
     8581 ccccatcaag ggaaagccca atcttcacat caatcggttt ttcacctgta ccgtacagag
     8641 tgattccacc tggagcagca gggacataca ccgttccctg atactcacca ggcatcacgg
     8701 caatatactg gcgcttgttg gtgcgcttga taattgccgc atctaccgcc gcctgaatcg
     8761 tggtatgcgt tacaccttga gtacccgccg ggccgacaac aaagtcaggt tgcgcaggca
     8821 gggttatcgg ggacggattc cacgctgcag cacctggtgt cagggatgca aaatagtgtt
     8881 gcgcatcgaa attcagcgct tcttttgccg acagaatcgg gcgagaagag gtaccaggcg
     8941 cagtttgatc agaaggacgt tgatcgggtg gtgttgagct acaggcggtc agcgtcacgc
     9001 caaaagccaa tgccagcgcc agacgggaaa ctgaaaatgt gttcacaggt tgctccgggc
     9061 tatgaaatag aaaaatgaat ccgttgaagc ctgctttttt atactaagtt ggcattataa
     9121 aaaagcattg cttatcaatt tgttgcaacg aacaggtcat tatcagtcaa aataaaatca
     9181 ttatttgatt tcaattttgt cccactccct gcctctgtca tcacgatact gtgatgccat
     9241 ggtgtccgac ttgtgcccga gaagatgttg agcaaactta tcgcttatct gcttctcata
     9301 gagtcttgca gacaaactgc gcaactcgtg aaaggtaggt ggatcccctt cgaaggaaag
     9361 acctgatgct tttcgtgcgc gcataaaata ccttgatact gtgccggatg aaagcggttc
     9421 acgacgagta gatgcaatta tggtttctcc gccaagaatc tctttgcatt tatcaagtgt
     9481 ttccttcatt gatatcccga gagcatcaac atgcaatgtt gtagggatgg caatttttac
     9541 gcctgttttg ctttgctcga cataaagata tccatctacg atatcagacc acttcatttc
     9601 gcataaatca ccaactcgtt gcccggtaac aacagccagt tccattgcaa gtctgagcca
     9661 acatggtgat gattctgctg cttgataaat tttcaggtat tcgtcagccg taagtcttga
     9721 tctccttacc tctgattttg ctgcgcgagt ggcagcgacc gggtttgttg ttatatggcc
     9781 ttcagctatt gcctctcgga atgcatcgct cagtgttgat ctgattaact tggctgatgc
     9841 cgccttgccc tcgtctatgt atccattgag cattgccgca atttcttttg tggtgatgtc
     9901 ttcaagtgga gcatcaggca gccccctcct tattgcttta attttgctca tgtaatttat
     9961 gagtgtcttc tgcttgattc ctctgctggc gaggattttt tcgtagcgat caagccatga
    10021 atgtaacgta acagaattat cactgttgat tctcgctgtc agaggcttgt gtttgtgtcc
    10081 tgaaaataac tcaatgttgg cctgtatagc ttcagt
//
LOCUS       DAZXUL010000136        10066 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZXUL010000136 DAZXUL010000000
VERSION     DAZXUL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10066
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.136"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            23..976
                     /locus_tag="ACH3L1_003549"
     CDS             23..976
                     /locus_tag="ACH3L1_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFT4078146.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            1185..2150
                     /gene="lipA"
                     /locus_tag="ACH3L1_003550"
     CDS             1185..2150
                     /gene="lipA"
                     /locus_tag="ACH3L1_003550"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFT4078147.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2251..2454)
                     /gene="tatE"
                     /locus_tag="ACH3L1_003551"
     CDS             complement(2251..2454)
                     /gene="tatE"
                     /locus_tag="ACH3L1_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFT4078148.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2583..3371)
                     /locus_tag="ACH3L1_003552"
     CDS             complement(2583..3371)
                     /locus_tag="ACH3L1_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFT4078149.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            3464..3847
                     /gene="crcB"
                     /locus_tag="ACH3L1_003553"
     CDS             3464..3847
                     /gene="crcB"
                     /locus_tag="ACH3L1_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFT4078150.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(3901..4110)
                     /gene="cspE"
                     /locus_tag="ACH3L1_003554"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3901..4110)
                     /gene="cspE"
                     /locus_tag="ACH3L1_003554"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFT4078151.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4285..4845)
                     /gene="pagP"
                     /locus_tag="ACH3L1_003555"
     CDS             complement(4285..4845)
                     /gene="pagP"
                     /locus_tag="ACH3L1_003555"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFT4078152.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            5434..6819
                     /gene="dcuC"
                     /locus_tag="ACH3L1_003556"
     CDS             5434..6819
                     /gene="dcuC"
                     /locus_tag="ACH3L1_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFT4078153.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(6860..7201)
                     /locus_tag="ACH3L1_003557"
                     /pseudo
     CDS             complement(6860..7201)
                     /locus_tag="ACH3L1_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(7539..8048)
                     /locus_tag="ACH3L1_003558"
     CDS             complement(7539..8048)
                     /locus_tag="ACH3L1_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFT4078154.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            8220..8456
                     /locus_tag="ACH3L1_003559"
     CDS             8220..8456
                     /locus_tag="ACH3L1_003559"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4078155.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            8431..10062
                     /locus_tag="ACH3L1_003560"
                     /pseudo
     CDS             8431..10062
                     /locus_tag="ACH3L1_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         2627 a         2363 c         2354 g         2722 t
ORIGIN      
        1 tgaataaaat tggagtaagt gcgtggatag taataatcaa attgaaccct gtttaagcag
       61 aaagtcgtca gaaggtaaac cacaaatatt tacaacctta cgaaatattg atcttaacct
      121 tctgactatt tttgaagctg tatatgtaca taaagggatc gttaatgcag cgaaagtgct
      181 taatctgacc ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta tattccctga
      241 cccattgttt attcgcaaag gccagggtgt cactcctacc gcatttgcga tgcatctaca
      301 tgagtatatc agtcagggcc ttgagtccat tcttggcgcg ctggatatcg aaggaagcta
      361 tgataagcaa cgaacgataa ctattgcgac cactccctcg gtcggagccc tggtccttcc
      421 tgtcatctat cgggcgatta aaactcacta tccgcagctt ttactgcgca acccgcccat
      481 cagcgacgcg gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatcg ataacatgtt
      541 ttgcaccaat cgtacggtgc aacatcatgt tctgttcacc gacaatatgg tgttaatttg
      601 ccgcaaggga aatccactac tctctttaga agatgacaga gagactatcg acaacgctgc
      661 gcatgtactt ctgttaccgg aagggcaaaa tttcagcggt ctgcggcaga gagttcaaga
      721 gatgtttccg gaccggcaaa tcaatttcac cagctacaac attttgacaa tcgctgcact
      781 ggttgccaac agtgacatgt tagcgattat tcccagccgt ttttataacc tgtttagtcg
      841 ttgctggccg ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa tagatttctc
      901 tatccactac aacaaattca tcctgcgtga tcccatcctg cacggagtaa tagatgttat
      961 ccgtaatgca ttttgaaaaa gtattgttac agccagatca cagcgcagaa cgataaaatg
     1021 gcacaatcca acaacaattt tacattttcg cgaccgcttt ggctgctttc aggtccgttt
     1081 caatgatata ctgccagtcg ttaattcaaa aatagttgat aattacaaca atctattgaa
     1141 ttgaaacgct ttccttcgta attcgcaact ggaacacgca cgctatgagt aaacccattg
     1201 tgatggaacg cggtgttaaa taccgcgatg ccgataagat ggcccttatc ccggttaaaa
     1261 acgtggcaac agagcgcgaa gccctgctgc gcaagccgga atggatgaaa atcaagcttc
     1321 cagcggactc tacacgtatc cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt
     1381 ctgtctgcga ggaagcctcc tgccctaacc tggcggaatg cttcaaccac ggcacagcaa
     1441 cgtttatgat cctcggcgct atttgtaccc gccgttgtcc gttctgtgat gttgcccacg
     1501 gtcgcccggt agctcctgat gccaatgaac cagtgaaact ggcgcagacc attgccgata
     1561 tggcgctgcg ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc gatggcggtg
     1621 cccaacactt tgcggattgt attactgcca ttcgggaaaa aagcccgcaa atcaaaattg
     1681 aaactctggt gccggatttc cgcggtcgaa tggatcgtgc tctggatatt ctgactgcaa
     1741 cgcccccaga tgtgttcaac cataacctgg aaaacgtacc gcgtatttac cggcaggtac
     1801 ggcctggcgc agattacaac tggtcgctga agctgctgga acgctttaaa gaagcgcatc
     1861 cggaaatccc gaccaagtct ggtctgatgg tgggtctggg tgaaaccaat gaagaaatta
     1921 ttgaggtaat gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt
     1981 tgcagccaag ccgccatcac ctgccggttc aacgttacgt tagcccggat gagttcgacg
     2041 aaatgaaagc cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc
     2101 gctcttctta ccacgccgat ttgcaggcga aaggaatgga agttaagtaa tcctgtaata
     2161 attacttaca tctgtctgat aaaaaacccg ctttttgaag cgggtttttt gtatcgaaca
     2221 agatattgag ggagcgtcct gctcgccacg tcactcttta tgagagagct tttcagcctg
     2281 aagatcaacg tctgcgcctt ttttcgccgc agcatcgtca tcattcatcg ccttcttgaa
     2341 ccctttaatg gccgctccaa ggtctccgcc cagcgtacgt aacttcttag tcccaaacag
     2401 cagaacgacc agcgccgcaa ctaccagcag tttggtaata ctaatctcac ccatagatac
     2461 cttcttgaca tataaccggc aacagaacgc gccttaaaaa ccagagaata ttaacggtca
     2521 ttcgacgcgc gcacacaata gcaatctgta acaaggtgaa tcaagcgccg agtaaaaaaa
     2581 catcataata attgcggcgg cgcaaagcga cggttgttta agatgggcag ttgcgcgcgc
     2641 acctgacgca cacgttcggg cgtcacttcc gccataatga gtgcaggcat ttctgacgct
     2701 gccgcaatgg tgacgccaaa gggatctata atccggcttt gcccgatatt tttgttcccg
     2761 cactcccccg ccgccaccat ataacaggtg gtatccagcg cacgagcggc aagcaacgtt
     2821 gaccagtgat gctctttgag cggcccacga acccaggcgg caggaagtac caggatttca
     2881 gctccctgta aagcctgtgc cagcgccagc tctggaaagc gtaagtcata acaggtcatc
     2941 agaccgacct tcatcccttc cacctccagt aacggagcta tttcattacc agcatcaaca
     3001 tggcgtgatt cctgaatggc aaatgcatca tagagatgca gtttggcata acgggcgacg
     3061 atgtttcctg cctgaagtgc caccagcata ttccatgcgc gccccggcgt tgaaggaaga
     3121 tgaatcgtca gaattgtcgt catcatgtta cgtttacttt ctcgccgtaa acgtccgagg
     3181 aattcgcctt ccagcagctg tgctgattta accgatagat ctgcatcatg atcatcgcgc
     3241 gccagcaatg cttccggcag gacaaacagc gatacgtcgt tttccgccgc ctgcgccatc
     3301 aacgaggcac aaatctcagc gttcttttcc cacacagatg taacagcaaa ctgtcctgca
     3361 gcaaccagca ttgccacctc ctgtcagagt cgtaaaattc gccaacgacg attattcggc
     3421 gttacactta tcactcatac aaatcaaata gcaggatttt gcagtgttac aacttctttt
     3481 agcagttttt attggcggtg gtacgggaag cgtagcgaga tggctgttaa gtatgcgatt
     3541 taacccgctg catcaggcga ttccgttggg gacgctggca gcaaacctga ttggggcatt
     3601 catcatagga atgggattcg cctggttcag caggatgacg aacattgatc cagtgtggaa
     3661 agtattaatc accaccggat tttgtggcgg tctaacaacc ttctcaacat tttcggcaga
     3721 agtggtgttt ttgttacaag agggccgctt tggctgggca ttactgaacg ttttcgtcaa
     3781 ccttctgggg tcttttgcca tgaccgcact ggcattctgg ctgttttcgg cctcaaccgt
     3841 acactaaaga ccaaaaaaaa cccgctgatt aagcgggttt tgaattcttg ctgacgtatc
     3901 ttacagagcg attacgtttg cagcagaagg gcctttggca ccgttagtga tttcgaactc
     3961 tacgcgctga ccttcagcaa gagttttaaa accattagtc tggattgcag agaagtgtac
     4021 gaacacgtct ttgctgccgt cttccggagt aatgaaaccg aatcctttgg actcattaaa
     4081 ccacttaacg ttacctttaa tcttagacat caaaattacc tttacatgaa aaatagacac
     4141 aaatgctgtg tcggttatca gtacaccaat tgtggtacgc tttgtccagt gcaactttac
     4201 taaaaagtga taaaagtcgc gttatttttg atgttgctta ttttagttat tgattttaaa
     4261 gaagttacta aaacttcatt tgtctcaaaa ctgaaagcgc atccaggcaa agtacacatt
     4321 gccattgttg taggtacccg gaatgtaggt catctgaaaa gtcactgggc cataacccac
     4381 ggaggccaat ggcagtagaa ccgggagagg gatgtaattc cagttatcgc gtgccgttac
     4441 gccagcggtg aatcccagac ctaaatgaaa attttcatcc gccagcggtc gccaggtact
     4501 ttcccatccg tatccggcaa tcggttccca tttgttccac gagtccttaa atgccatggc
     4561 atacaggcca tgccagtttc ctttttcatc ccaacgcgac aggccaaaac cgccacccca
     4621 cggtcgctcg ttatagcgat cggttttttc tttgtcgtaa gcgaaacgtg catgccaggt
     4681 gatggcagga atatataaat cataatgttc aggctgttgc caggtttgtg caatattttc
     4741 tctaaacgtt gtcatcctct catctgcgtt agcaaaaact ttaccaacgc tgattaactg
     4801 aataaaaaca aaggaaaaga tagcgacata tttactcacg ttcatttgtg accataaaac
     4861 atttatcaaa aatctactac tagcatagca aagctacaat taacataacc ttaatagacc
     4921 caacataaag aataatctga atagctgctc tatttatttc cgcttgttca agttaaatat
     4981 ttccgcgcat cgattaaaga tgatgcgtcg ttttctgcga tgggaatagt caaaaaagaa
     5041 aaaccaagtc ttttttgatg acaaaatgca atcaaggaaa aattatttta tttttttaac
     5101 gagatacaac aatcatctta acgaagtata taatatatat cgtcaaccca tgctcatatt
     5161 agattatttt ttgagcgcac agccgcagct aacaccattg caattaacaa atttgcatca
     5221 atccaccatc aatttacaca cattattatg tgataattgc caaccgctaa atatgcgttt
     5281 tgttatctat gtataaaaac agcaacttca atgtcttaat ggcagttttt cttgatttta
     5341 atcagcattc atcgccaatt tattgggcat attttttcct taagctttag gaagttttta
     5401 ttatttactt tgggcctgga gacaggaaaa attatgctga cattcattga actccttatt
     5461 ggggttgtgg ttattgtggg tgtggctcgc tacatcatta aagggtattc cgccactggt
     5521 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
     5581 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
     5641 atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggcttt
     5701 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
     5761 ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt tgcttgtctg
     5821 atgtcactgg ccgtctcttc cgcaaccggt ctgggggttt tgctgatggc aaccctgttt
     5881 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagcag ctgccatttg tgcctccccg
     5941 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca ggcttccgaa
     6001 atgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc
     6061 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
     6121 catgaaatgt tagatgtcag tgaaatcacc accactgccc ccgcgtttta tgccattttg
     6181 ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac
     6241 atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc
     6301 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg
     6361 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
     6421 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     6481 ctgatgctgg tattggtgat tctgaccatg ctggcggcag tcacgaccgg ttcaggcaat
     6541 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac
     6601 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     6661 gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta
     6721 aaacgcacct cggtgccggt gcttgttggg ctagtgattg ttatcgttgc tacagagctg
     6781 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     6841 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc
     6901 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     6961 cagtggtgcg gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     7021 gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggcagtt
     7081 cgtctttggg ttcaccgcga gcataggcat taaacatctc atcaatttgc ttctggctgg
     7141 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     7201 gtgtattgat tcacttgaag tacgaaaaaa ccgggagaac attggattat tcgggatctg
     7261 atgggattag atttggtggg gcttgcaagc ctgtagtaca aattttaggc gttaaccaat
     7321 gaaacgcgaa agatagtaaa aaattgcttt tgtttcattg aaaaaacgaa aaacaaaaac
     7381 actataaatc atttcaatca tagcttcaaa aaacgttcaa aaccgataac aaccaggctg
     7441 tcatcaaatg actcatatca caaatcagct catgccgttt aggtatgtta catgtgtgat
     7501 tatgtgaggt gaagtatgtt ttagctggct catggttgtt atacggcttt tttcgcatct
     7561 tgtggttcca acaaaggagc tacaggctct tcctgtttat gaacaccata cattgacaaa
     7621 atctcttggc gagtttctgg aggtaattta tgcagcgcca aagctgtttg tagtgtttgg
     7681 cgatcaatac ccagcgttgc caatagggtg tcaacggaga tggaggggcg ttcttgcatt
     7741 aatccggaaa gcccatattt agccatgttt agctctatct ctcggacttc attttctcct
     7801 agtttattga ttaacaggga gaacattttc aatgtatcag cattcatttc ttcgatagca
     7861 attttttgca tagccggtat tgccgggata tattcaaccc caggcccgcg acgcccttct
     7921 tgtgaacgct tcggccatcc ttctttcttt gctcgtttat gcaccccctg tattgtccca
     7981 ggcattccat ccagttcagt aatttgtttg ggtgtcatcc actggtttat ctcatttgcg
     8041 cttttcataa actaagttta ccagatttat ctatataaaa atcaatggat tacaaaataa
     8101 accacattaa ctgatataaa ccggtttggt ttatttggta atgtataaaa cagataatca
     8161 agacgaatta tttgttttgg taacccttta tcatacgcaa tatggttggt gtgcgaaaga
     8221 tgagtaacga ttggcatcgg gccgacgtga ttgcggccct caaaaaaaca ggaacccctc
     8281 tggcaaaatt gtccagacgt tcaggttatt caaccgggac gcttgcaaat gcattagtca
     8341 ggccatggcc taaaggtgaa cgcattattg ctgacgcctt aaacatgaaa cctgaggaaa
     8401 tttggcctag tagatatcta agggatcaca atgagagagt ggatatcagc aattgaatgc
     8461 atgggtttat ctggtttacc tggttcatta atgggggttc acaaaagagc tcagagagaa
     8521 ggatggctca agagaagaag agaaggaatt agaggggttg cttacgaata ccatatatct
     8581 tctcttccta acattgctcg agctgaagtt ttgctccaac aaggaaaatt taaaaccagt
     8641 cagggatgtt ttgaaatcgc ccgccccacg ctggaagctc atgattatga tcgtgaggca
     8701 ctatggagca aatgggataa agccagcgat tcccagcgca gcctggctga aaaatggttg
     8761 ccctcaattc aggccacaga cgaaatgctg aaccagggga tttcaacgaa aacggctttt
     8821 gcgaccgttg cagggcatta ccaggtcagc gcatccactt tgcgggacaa gtattaccag
     8881 gtacagaagt ttgcgaagcc tgactgggcg gctgcacttg ttgatggacg tggagcatcc
     8941 cgtcgcaatg ttcacaaaag tgaatttgac gaggatgcct ggcagtttct gattgcagat
     9001 tatctgcgac cggaaaaacc cgccttccgc aaatgttatg agcgtctgga actggcagcc
     9061 cgcgagcatg gctggagtat tccctcccgt gccacggcct ttcgccggat tcagcaactg
     9121 gacgaggcaa tggttgttgc ctgtcgtgaa ggtgaacatg cactgatgca tctgataccg
     9181 gcacagcagc gaactgtgga acacctggac gccatgcagt ggatcaacgg cgacggttat
     9241 ctgcataacg tctttgtacg ctggtttaac ggtgatgtga tccgtccgaa aacatggttc
     9301 tggcaggatg tgaaaacccg aaaaattctg ggctggcgct gcgatgtgag cgagaacatt
     9361 gattcaattc gcctctcgtt catggatgtt gtgactcgct acagtatccc ggaggatttt
     9421 cacatcacca ttgataacac ccgtggtgct gcgaataaat ggctgacggg aggcgcgccc
     9481 aatcgctacc gctttaaggt aaaagaggac gatccaaaag gactgttttt actgatgggt
     9541 gcgaaaatgc actggacaag cgttgttgcc ggtaaaggct ggggccaggc aaaacctgtt
     9601 gaacgtgctt tcggtgttgg tgggcttgag gaatacgttg ataagcatcc ggcactggct
     9661 ggcgcatata cggggccaaa tccgcaggca aaacctgata actatggcga ccacgctgtt
     9721 gatgcagagc tgtttctgaa aacctttgcc gaaggtgtgg cgatgttcaa tgccagaaca
     9781 ggccgtgaaa cagaaatgtg cgggggcaaa ctctcgtttg atgatgtttt cgagcgtgaa
     9841 tacgccagaa cgattgtgcg taagccaacc gaagaacaaa aacggatgct gttactgcct
     9901 gccgaggcgg tgaacgtttc acgcaaaggc gagtttacgc ttaaagttgg cggctccctt
     9961 aaaggcgcga aaaacgttta ttacaacatg gcattaatga atgccggagt gaaaaaagtt
    10021 gtggtcagat ttgatccaca gcagttacac agcacggttt attgag
//
LOCUS       DAZXUL010000137         9976 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZXUL010000137 DAZXUL010000000
VERSION     DAZXUL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9976
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.137"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            41..1170
                     /gene="ompN"
                     /locus_tag="ACH3L1_003561"
                     /pseudo
     CDS             41..1170
                     /gene="ompN"
                     /locus_tag="ACH3L1_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            1311..1745
                     /gene="uspF"
                     /locus_tag="ACH3L1_003562"
     CDS             1311..1745
                     /gene="uspF"
                     /locus_tag="ACH3L1_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFT4078156.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1921..2856
                     /gene="ttcA"
                     /locus_tag="ACH3L1_003563"
     CDS             1921..2856
                     /gene="ttcA"
                     /locus_tag="ACH3L1_003563"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFT4078157.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2985..4352)
                     /gene="dbpA"
                     /locus_tag="ACH3L1_003564"
     CDS             complement(2985..4352)
                     /gene="dbpA"
                     /locus_tag="ACH3L1_003564"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFT4078158.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(4382..4555)
                     /gene="ynaL"
                     /locus_tag="ACH3L1_003565"
     CDS             complement(4382..4555)
                     /gene="ynaL"
                     /locus_tag="ACH3L1_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFT4078159.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4830..5813)
                     /gene="zntB"
                     /locus_tag="ACH3L1_003566"
     CDS             complement(4830..5813)
                     /gene="zntB"
                     /locus_tag="ACH3L1_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFT4078160.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5827..5874)
                     /gene="ydaN"
                     /locus_tag="ACH3L1_003567"
     CDS             complement(5827..5874)
                     /gene="ydaN"
                     /locus_tag="ACH3L1_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFT4078161.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6068..7300
                     /gene="dgcM"
                     /locus_tag="ACH3L1_003568"
                     /pseudo
     CDS             6068..7300
                     /gene="dgcM"
                     /locus_tag="ACH3L1_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(7321..7884)
                     /gene="smrA"
                     /locus_tag="ACH3L1_003569"
                     /pseudo
     CDS             complement(7321..7884)
                     /gene="smrA"
                     /locus_tag="ACH3L1_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(8138..9592)
                     /locus_tag="ACH3L1_003570"
     CDS             complement(8138..9592)
                     /locus_tag="ACH3L1_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFT4078162.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(9589..>9976)
                     /locus_tag="ACH3L1_003571"
     CDS             complement(9589..>9976)
                     /locus_tag="ACH3L1_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFT4078163.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         2753 a         2487 c         2267 g         2469 t
ORIGIN      
        1 ggggataaaa ggcaaataaa ataactaaag gatttattca atgaaaagaa aagtactggc
       61 acttttaatt cctgccctgc tcgccgcagg tgctgcacat gcagccgaag tttataataa
      121 agacggcaac aaattagatc tgtatggcaa agttgatggc ctgcattatt tttctgataa
      181 ttcagcgaaa gatggcgacc agagctatgc gcgtctgggt tttaaaggcg aaacccaaat
      241 taacgatcaa cttactggct acggtcaatg ggaatacaat attcaggcaa acaacactga
      301 atcttcaaaa aaccagtcat ggacccgtct ggcatttgcc ggactgaaat ttgcagatta
      361 cggttctttc gattacggac gtaattatgg cgtaatgtac gacatcgaag gctggaccga
      421 tatgctgcct gaatttggcg gtgactctta taccaatgca gacaacttta tgactggtcg
      481 agccaatggc gtcgcgactt atcgtaatac tgatttcttc ggtctggtaa atggtctgaa
      541 cttcgcggtg cagtatcaag gtaacaacga aggagccagt aatggtcagg aaggcaccaa
      601 taacgaacgc gatgttcgtc atgaaaacgg tgacggctgg ggtctttcca caacatatga
      661 tttaggcatg ggctttagcg ctggtgcggc atacacctct tctgaccgca ccaatgacca
      721 ggttaaccat actgcggcgg gtggtgataa agcagacgcg tggactgctg ggctaaaata
      781 cgatgctaac aatatttacc tagcaaccat gtattcagaa acgcgtaata tgaccccgtt
      841 tggcgacagc gattatgcag ttgcgaacaa aacccagaac tttgaagtta ctagtaccag
      901 tttgattttt gtctgcgtcc ggcagtctct ttcctgatgt ctaaaggccg tgaccttcac
      961 gctgctggtg gtgcagacaa cccggcaggt gttgatgata aagatctggt taaatacgcc
     1021 gatgttggcg cgacttacta tttcaataaa aacatgtcca cctatgttga ctataaaatc
     1081 aacctgttgg atgaagatga cagtttctac gctgccaacg gcatctctac cgatgatatt
     1141 gtcgctttag gtctggttta tcagttctaa atcctcctgc ccgctgttat ggcgggcttt
     1201 ttctgcttat tcttcctcct ttgatctaaa ttaaaaatgt gaactccgtc attacacaaa
     1261 aagtgtcatc tggcgttaca ctttatgcgg atactaaaac aggaggtttt atgaacagaa
     1321 cgattcttgt ccctatcgat atttcagatt cagaattaac tcaacgcgtg attagccacg
     1381 ttgaggcaga ggcaaagatt gatgatgcag aggttcattt cctgacggta ataccttcac
     1441 tgccctacta tgcctctctg ggtttagcgt attccgcaga attaccggca atggatgacc
     1501 tgaaagcgga agccaaatcg caactggaag agatcattaa aaaatttaaa ctgccaaccg
     1561 acagagtgca tgtccatgtt gaggaaggct cgcccaaaga ccgcattctg gaattggcga
     1621 agaagatccc cgctcatatg atcatcattg cttcccatcg accggatatc accacttatc
     1681 tgctcggttc caacgccgca gctgtagtgc gtcacgcaga gtgctccgtg ctggttgtgc
     1741 gctgacacta acgcccgcac attgctgcgg gctttttgat tcatttcgca aatgtgctga
     1801 cattttcccc tctaatccgt accatacacg ccacagtttt tatatcagat ttcttatgct
     1861 gagcgttccg gcatgacgca tactcttctg atgccatata acgaattgag tcgcttttaa
     1921 atgtcgcaaa atcaagaaat tagtaagaaa gaacaataca acctgaacaa attacaaaaa
     1981 cgtctgcgtc gtaacgtggg cgaagccatt gctgacttca atatgattga agaaggcgat
     2041 cgcatcatgg tttgcctctc cgggggtaaa gacagctata ccatgctgga gattctgcgc
     2101 aatttgcagc aaagcgcgcc aatcaatttt tcgctggtgg ctgttaacct cgatcaaaag
     2161 caaccgggct tcccggaaca cgttctgccc gagtatcttg aaaagctggg cgttgagtac
     2221 aagattgttg aagagaacac ttacggtatc gtgaaagaga agattccaga gggcaaaacc
     2281 acttgctcac tgtgttctcg gctacgtcgc ggtatccttt atcgcaccgc aacggaactg
     2341 gggacgacga agatcgcgtt gggtcaccat cgtgacgata tcctgcaaac gttgttctta
     2401 aatatgttct acggcggcaa gatgaaaggt atgcctccga aactgatgag cgatgatggc
     2461 aaacatatcg ttattcgtcc actggcctac tgccgcgaga aagatattca gcgatttgcc
     2521 gatgcaaaag cgttcccgat tattccgtgc aacctgtgcg gttcacagcc taacctgcaa
     2581 cgtcaggtga ttgctgacat gttgcgtgac tgggataaac gttatccagg gcgtatcgag
     2641 acgatgttca gcgcgatgca gaatgtggtg ccgtcgcatc tgtgcgatac caacctgttc
     2701 gatttcaaag gcattaccca cggttctgaa gtggttaacg ggggtgatct ggcgtttgat
     2761 cgcgaagaga tcccactaca accggcgggc tggcagccag aagaagatga aaatcagttg
     2821 gatgagttac ggctgaatgt ggttgaagtg aaataaccag gatagcgccc gatgcgcaag
     2881 cgtatcgggc tactcttatg gaggccggat aagacgcggc cagcgtcgca tctggcaatc
     2941 ccgaataaga tgtttactct tgcacccggc aattcaacat ttcattattt taataaccgc
     3001 acccggcacg tttttccttt aatcttcccg ccctgtaact gtttccatgc tttatgagca
     3061 acagcctgac ggaccgcgac atagacatgc gccggatgca cggcgatttt gccaatatct
     3121 gcgccatcaa gcccgatatc tcctgtcagt gcacctaata catcacccgg gcgcattttg
     3181 gcttttttcc cgccatcgat acacaacgtt gccatttctg cttccagcgt cacaatggaa
     3241 ctattagctg gcggcgtttg ccagttaagt tttatctgca acatgtcaga aatgatattg
     3301 gcccgctgtg cttcttccgg agcacagaaa ctgatcgcca gaccgctatt tcctgcacga
     3361 gctgtacgac cgatgcgatg tacatgaact tcagggtccc acgccagctc aaagttcacc
     3421 accagctcaa gcgatttaat atccagacca cgcgcagcaa catcagtcgc aaccagtaca
     3481 cgggcgctac cgttagcaaa acgtaccagg gtctgatcgc gttgctccag atcgccgtgt
     3541 aacgacaatg cactttgctc tacttcattc agcgcgtcgc agacagcctg gcaatctttt
     3601 ttggtattgc aaaacaccac acaagaggat ggctgatgca agcttaataa ccgttgcaac
     3661 agaggaattt tgcctttact ggatgtctca taaaattgtt gttcaatggg tggcaaagca
     3721 tctgttgagt caatttcaat cgccaaagga tcgcgttgca ctcgtccgct gattgcggcg
     3781 atggcttccg gccaggttgc cgaaaacaga agcgtctgtc gagatgcagg cgcaaaacgg
     3841 atgacatcat caatggcatc gctaaatccc atatccagca tgcggtcggc ctcatccatc
     3901 accaaagtat tcagcgcatc cagtgatacc gtgccttttt gcaagtgatc cagcaaacga
     3961 cccggcgttg ccacgataat atgcggcgca tgttgcagcg aatcacgctg cataccgaac
     4021 ggttgaccac cgcacaacgt caaaattttg gtatttggca gaaaacgcgc cagccgacgc
     4081 aattcacctg ccacctgatc cgccagttca cgcgtaggac acagcactaa agcctgggtt
     4141 tgaaatagcg acgcatcaat ttgctgtaac aagccgaggc caaaagccgc cgttttgccg
     4201 ctgccggttt tcgcctgcac gcgaacatct tttccggcaa ggatcgccgg aagcgcggcg
     4261 gcctgcaccg gcgtcatggt taaataaccc aactcattaa ggttcgtgag ttgggcggga
     4321 ggcaaaacat tcagggtaga aaaagcggtc acaatctatt ctcgtggtca tcgacgcaaa
     4381 gttagcaggc gcgtatcctc gcagatctac gctcacgatg cgacaattta atcggttctt
     4441 catcgggtgg tgggtcaggc atgggttgcg ggcgagggat cggatcgggc actggaacag
     4501 gatcgccagg aatcggttca gggacaggaa tttgcaaata aataagtgtc gtcatatttc
     4561 cctccggtca ttgggtggac tcttaaaggg tagacgctga taaataacag gcaaaaaaaa
     4621 gccgactcat caaagtcggc gtcgtacgaa tcaattgtgc tatgcagtaa ttcaaaaaag
     4681 gaagtaagac aatatggagc gcaacgccca tcgcttgacg ttgcattcac ctgcaaggga
     4741 gatattgccc tgaatgggta gagagtttat tgacttcgct caaactttgc ggcgtttttg
     4801 tatacagaca gccggaaaaa ttgcttttgt tacaaccatt tactacggtg caaccataaa
     4861 gcaacaccac caataagaac aactaacaga atacaaaaaa ttgaaaatcc gaattgccac
     4921 ccgccgccag ggatcccacc aaggttgacg ccaaataacc cggtcagaaa ggtactgggt
     4981 aaaaagacca ttgccatcaa cgacattgta taggtacgac gagctaaatt ttcctgcatc
     5041 acctgagcga tttcatccgc catcacgcca gtccgtgcta tacaggcgtc gatttcgtca
     5101 aggccgcgcc caaggcgatc ggcaatatcc tgcatccgac ggcgttggtc atcgctcatc
     5161 cacggcaaac gttcactggc aagacgagca taaacatcac gttgcggtgc catatagcga
     5221 cgcatcacaa ttaattgttt gcgcagcaga gccaggaatc cacgcggtgg aatttgctga
     5281 tcaaggagat tatcttcaag gtcgataatt ttatcgtgca gctgctcgat aaattcactg
     5341 gaatgatcgg tcaacgcatc gcacacatcc accagccatc ccccgcaatc ggtcggaccc
     5401 gtgccctctt ccagatcgct caccacatcg tccagcgcca gcactttgcg ttgtcgggtc
     5461 gaaacaatta accgcccgtc catatataca cgcatggcga ccagttgatc ggggcgttca
     5521 tcggtgctgc cgtttataca gcgcaatgta atcagcgtgc cttcaccgaa acggctgact
     5581 cggggccgtg tgctctcgcc cgccagcgca tcacgtacgt tattgggaag cagcggtgtt
     5641 gtcgccagcc attgggcgct atcatggtgt acataattaa ggtggagcca gcagggatgc
     5701 gcttcatcaa tcacatctgt attttccagc ggtttaacgc cgcctctacc atccagcatc
     5761 caggcaaata ctgcatccgg gacattaacg tccgatccct taatcgcttc cactgtgcct
     5821 ccatcatcaa cgcattattt tgtagtctag ccttctggcc ctgttacgca acatctcatc
     5881 accccattac cctgaaatga ttaataaaat tctgtctaaa ttgaatacaa aaagcaaaat
     5941 gcttttccgt atacaaaccg tgtgaagtgt taaatagcgt ctatcattat cagaattatc
     6001 tgatcatatg acgtggcttt tttgcgatcg gatagcaaca aaaattgata aaaataacgg
     6061 gatctcaatg attacgcaca acttcaatac cctggactta ctcaccagtc ctgtctggat
     6121 tgtttcgccc tttgaggaac agttaattta tgccaatagc gcggcgcgac tgttgatgca
     6181 agacctcacg tttagtcagc tacgaaccgg accctattcc gtctcctcac aaaaagaact
     6241 gccgaaatac ctctccgatc tgcaaaacca acacgatatt atcgaaatcc tcactgttca
     6301 gcgtaaagaa gaggaaacag cattaagctg tcggcttgtt ttgcgagagc tgacagaaac
     6361 agaaccggtg attattttcg aaggtatcga agcgccggca acgctgggtt taaaagccag
     6421 tcgctcggca aattatcagc gcaaaaaaca aggtttttat gcgcgctttt ttctgactaa
     6481 ctctgcacca atgttgttga ttgacccgtc acgagatgga caaatcgtcg atgctaacct
     6541 cgccgcgctc aatttctatg gttataacca tgaaacgatg tgccagaaac atacctagga
     6601 aataaatatg cttgggcgtc gcgtcatgcc tatcatgcat gaaatttcgc atttacccgg
     6661 cggtcataac cctttgaatt ttgttcataa actggcggat ggatctactc gtcatgtgca
     6721 gacctatgcc ggaccgattg aaatttatgg cgacaagctc atgttatgta ttgtgcatga
     6781 tattactgag caaaaacggc tggaggagca gctggaacat gctgctcacc atgacgcgat
     6841 gaccggatta ctgaatcggc gacagtttta tcacattacg gaaccaggcc aaatgcagca
     6901 tctcgccatc gctcaggatt acagcttgtt gctcatcgac accgatcgtt ttaaacacat
     6961 taacgatctc tatgggcatt ctaaaggtga tgaggtgtta tgcgccctcg cccgccccct
     7021 cgaaagttgc gctcgcaaag gcgatttggt gtttcgttgg ggaggcgaag agtttgtctt
     7081 attgctacca agaaccccac tggataccgc gctttcgctg gctgaaacta tccgcgtaag
     7141 cgtggcaaaa gtgagtattt cgggcttacc acgctttacc gtcagcattg gtgtggcgca
     7201 tcacgaagga aatgaaagca tcgatgaact gtttaaacgc gttgatgatg ctttgtatcg
     7261 ggcgaaaaat gatggacgca accgcgtgct ggcggcataa gccgcggatg cgtctcgaga
     7321 tcaacgactg cgcttagcgt ggcgctccca gttttcttgc ttcgcctgcg ccgttttacg
     7381 tagtgcgacg taacacgccc cgctgccgcc atgatgcggt agcgcggtgc aatatgcctg
     7441 aacatcatca aattcggtca gccagcgcgc cacatagctg cggacaatat tggcatgtga
     7501 tttatcttcc cgccctttac catgaataat cagcacatta cgcaaaccat ccgccagggc
     7561 ttgttgaatg aaactgaaca ccattttgcg gcactcttcc accggctggc gcaaaagatt
     7621 caggctcgcc tgttgcggat atttaccact gcgcagctta tccagcaccc catgctacaa
     7681 cccttcctgc cgaaactcca gcggctgact tagcgggatg atgtcgagaa atccggtagt
     7741 gaggaaatta tcaagctgca gcgtgtcgat acgctgcggc gcacgttggt tacgcgttgg
     7801 atgccagtgg acatctgtag cacgtttcag cggctggaca tcttccatgg cgtcaagaaa
     7861 cagcgatttg tcgtcaaggt tcatgtaacc tcctatgcac cttcacacgt cgtgattcag
     7921 acgggttaat tgttcatact tactgcatcg ttcccttcac agattgaaac aatactctac
     7981 ttcatacctg tgagtatctg cgcaggcggg gcattttata ctagcctgtt agcagtatcg
     8041 aacagtttag cccgagttta attcagcaca ggctccgatc aggatgattt attcatagca
     8101 ggaaccaaca aaaatgcgat aaataacaga ccactgatta gcagaaaggt accgaaaatg
     8161 ccatagtttg cgcctccctc agcatcaatt gactcaataa tttggttgaa gccagtattt
     8221 ccgatgtctg gtcacttcta ctgcgattaa aaaataaagc acttctggaa atgaagtcat
     8281 tcaccgtatg gggaacagaa accatttttt cacccattct cgaaaaatga aaattagttc
     8341 gattattgag aatagagcca cacagggcaa taccaatcgc gcccccaaga ttacgtgtta
     8401 aattaaatac accagaggct aacttcagcc gttcttttgg aattcccccc agcgttaatg
     8461 ttacgatggg ggccatcgca aactgctgcg aaatccctct aattgcctgt ggaaacagta
     8521 attcctgcca tccccattcg tgggttatag gggtaaaaag atacattgaa aacacaaatc
     8581 cgcccagccc cgccatcaac agccatcgca gatttatttt tttacttaac cagaagtaaa
     8641 agggaacaga aaataactgg aaaatacctg ttgtgcaaac ggcaaaacct atctcttcgg
     8701 catttaatcc gcgaatctgc cccaaaaaca caggtattaa atatacagta gagaatatcc
     8761 caacccctcc agaaaaagaa aaaaaacatc ccagagtgaa atttttatcc ttaaaagcat
     8821 gaagatccat tatcggattg gatattgtaa gtgtacgcgc agcaaataaa ataaaactca
     8881 ccaatgccag aactgatgtc agcaaaatag tattatcatc cagccatccc cagcgcgctc
     8941 cttcttcaag ggtatattcc agacatccca gagtcatggc caacaggata atactcggat
     9001 aatcagcaac tttcagtaaa gagagatcag gtttatcaaa attaaccaga aaaggaatac
     9061 tcaaaactaa atatatgcca ggtaatatat tgatataaaa tagccatcgc cagtcgaggt
     9121 tatcagtgag ccagcccccc agagttggcc ctagcgtcgg tgacagtgag gccagcgcac
     9181 tgactaccgc agcagccaac cccaactctt taccctggta ataaataaac gccatcgtga
     9241 aaactaaagg gatcatggat gcgccagcag ctccctgtaa agcacgaaac aaaatcatga
     9301 tctgaatatt ccatgcaagt ccacacgcga tactcattaa agtaaatatt cccgcggata
     9361 gagtaaaaag ccaacgagtg gaaaataccc gtgacagcca cccagagagc ggaataacaa
     9421 taatctcagc aattaaatag ctggtctgaa gccaggccat ttcgtcttta cctgcggaca
     9481 gaccaccacc tatttcattt agggatgacg atacaatttg aatatcaagc tgagcaataa
     9541 ataatcccat acacatactg gcgaaagcaa tatgtttacg catagacatc atctctcatc
     9601 aacactgacg gtgacggaca gtcccgggcg aagaagttgc tttaattcct ttgcgtcgtc
     9661 aaatgcaatc cgtaccggga cacgctgaac aattttggtg aaattgccag tggcattttc
     9721 tatcggaatc aagctgaaac tggctcctgt cgctggggaa aggctttcaa tgtgaccgtg
     9781 aaacacttcc cctttgagaa tatcagcacg gatctcagcc ttcattcctg gcttcattcc
     9841 cgctatttgg ttttctttat aatttgcatc aacccacaac tcactgactg gtactagtga
     9901 aactaacgat gtgccgcctt ccacccaact gcctgtatgg gcgctgcggt ttgcgacaat
     9961 gccatcaatc ggcgca
//
LOCUS       DAZXUL010000138         9762 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZXUL010000138 DAZXUL010000000
VERSION     DAZXUL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.138"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            383..1501
                     /locus_tag="ACH3L1_003572"
     CDS             383..1501
                     /locus_tag="ACH3L1_003572"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFT4078164.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="ACH3L1_003573"
     CDS             1567..1815
                     /locus_tag="ACH3L1_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFT4078165.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="ACH3L1_003574"
     CDS             1880..2248
                     /locus_tag="ACH3L1_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFT4078166.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="ACH3L1_003575"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="ACH3L1_003575"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFT4078167.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="ACH3L1_003576"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="ACH3L1_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFT4078168.1"
                     /translation="MQDLISQVEDLAGIEIDHITSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACH3L1_003577"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACH3L1_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFT4078169.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACH3L1_003578"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACH3L1_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFT4078170.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACH3L1_003579"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACH3L1_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2388 a         2704 c         2368 g         2302 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacatca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg ca
//
LOCUS       DAZXUL010000139         9745 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZXUL010000139 DAZXUL010000000
VERSION     DAZXUL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9745
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.139"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="ACH3L1_003580"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="ACH3L1_003580"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFT4078171.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="ACH3L1_003581"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="ACH3L1_003581"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFT4078172.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="ACH3L1_003582"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="ACH3L1_003582"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFT4078173.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="ACH3L1_003583"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="ACH3L1_003583"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFT4078174.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="ACH3L1_003584"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="ACH3L1_003584"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFT4078175.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="ACH3L1_003585"
     CDS             complement(6031..6360)
                     /locus_tag="ACH3L1_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFT4078176.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACH3L1_003586"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACH3L1_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFT4078177.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="ACH3L1_003587"
     CDS             7998..8171
                     /locus_tag="ACH3L1_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFT4078178.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACH3L1_003588"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACH3L1_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFT4078179.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2553 a         2279 c         2429 g         2484 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttg
//
LOCUS       DAZXUL010000140         9681 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZXUL010000140 DAZXUL010000000
VERSION     DAZXUL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.140"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(44..214)
                     /locus_tag="ACH3L1_003589"
                     /pseudo
     CDS             complement(44..214)
                     /locus_tag="ACH3L1_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACH3L1_003590"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACH3L1_003590"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFT4078180.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="ACH3L1_003591"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="ACH3L1_003591"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFT4078181.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="ACH3L1_003592"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="ACH3L1_003592"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFT4078182.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="ACH3L1_003593"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="ACH3L1_003593"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFT4078183.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="ACH3L1_003594"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="ACH3L1_003594"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFT4078184.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="ACH3L1_003595"
     CDS             5109..6242
                     /locus_tag="ACH3L1_003595"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFT4078185.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="ACH3L1_003596"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="ACH3L1_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFT4078186.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="ACH3L1_003597"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="ACH3L1_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFT4078187.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="ACH3L1_003598"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="ACH3L1_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFT4078188.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="ACH3L1_003599"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="ACH3L1_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFT4078189.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="ACH3L1_003600"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="ACH3L1_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFT4078190.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="ACH3L1_003601"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="ACH3L1_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFT4078191.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2480 c         2466 g         2270 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt t
//
LOCUS       DAZXUL010000141         9649 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZXUL010000141 DAZXUL010000000
VERSION     DAZXUL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.141"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="ACH3L1_003602"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="ACH3L1_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFT4078192.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1102..1185
                     /gene="yqiM"
                     /locus_tag="ACH3L1_003603"
     CDS             1102..1185
                     /gene="yqiM"
                     /locus_tag="ACH3L1_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFT4078193.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1207..1869
                     /gene="yqjA"
                     /locus_tag="ACH3L1_003604"
     CDS             1207..1869
                     /gene="yqjA"
                     /locus_tag="ACH3L1_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFT4078194.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="ACH3L1_003605"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="ACH3L1_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFT4078195.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /gene="yqjC"
                     /locus_tag="ACH3L1_003606"
                     /pseudo
     CDS             2403..2757
                     /gene="yqjC"
                     /locus_tag="ACH3L1_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2795..3100
                     /locus_tag="ACH3L1_003607"
     CDS             2795..3100
                     /locus_tag="ACH3L1_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFT4078196.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="ACH3L1_003608"
     CDS             3103..3507
                     /locus_tag="ACH3L1_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFT4078197.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="ACH3L1_003609"
     CDS             3497..3796
                     /locus_tag="ACH3L1_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFT4078198.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="ACH3L1_003610"
     CDS             3982..4278
                     /locus_tag="ACH3L1_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFT4078199.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="ACH3L1_003611"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="ACH3L1_003611"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFT4078200.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="ACH3L1_003612"
     CDS             5627..5995
                     /locus_tag="ACH3L1_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFT4078201.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="ACH3L1_003613"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="ACH3L1_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFT4078202.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="ACH3L1_003614"
     CDS             7203..7904
                     /locus_tag="ACH3L1_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFT4078203.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /gene="yhaL"
                     /locus_tag="ACH3L1_003615"
     CDS             7927..8091
                     /gene="yhaL"
                     /locus_tag="ACH3L1_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFT4078204.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /gene="cyuA"
                     /locus_tag="ACH3L1_003616"
     CDS             complement(8303..9613)
                     /gene="cyuA"
                     /locus_tag="ACH3L1_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFT4078205.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
BASE COUNT         2408 a         2523 c         2404 g         2314 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 taattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaa
//
LOCUS       DAZXUL010000142         9609 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZXUL010000142 DAZXUL010000000
VERSION     DAZXUL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.142"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(29..388)
                     /locus_tag="ACH3L1_003617"
     CDS             complement(29..388)
                     /locus_tag="ACH3L1_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFT4078206.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(385..660)
                     /locus_tag="ACH3L1_003618"
     CDS             complement(385..660)
                     /locus_tag="ACH3L1_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFT4078207.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(719..1843)
                     /locus_tag="ACH3L1_003619"
     CDS             complement(719..1843)
                     /locus_tag="ACH3L1_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4078208.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(1843..4578)
                     /gene="rbbA"
                     /locus_tag="ACH3L1_003620"
     CDS             complement(1843..4578)
                     /gene="rbbA"
                     /locus_tag="ACH3L1_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFT4078209.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(4575..5642)
                     /locus_tag="ACH3L1_003621"
     CDS             complement(4575..5642)
                     /locus_tag="ACH3L1_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFT4078210.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(5705..5818)
                     /gene="yhiY"
                     /locus_tag="ACH3L1_003622"
     CDS             complement(5705..5818)
                     /gene="yhiY"
                     /locus_tag="ACH3L1_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFT4078211.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(6008..7630)
                     /locus_tag="ACH3L1_003623"
     CDS             complement(6008..7630)
                     /locus_tag="ACH3L1_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFT4078212.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(7892..9481)
                     /locus_tag="ACH3L1_003624"
     CDS             complement(7892..9481)
                     /locus_tag="ACH3L1_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFT4078213.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWIYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
BASE COUNT         2486 a         2409 c         2123 g         2591 t
ORIGIN      
        1 tattctgcgt aatgtacgtc gctaaaattt ataaatgatt caaacgctca ttcagtgttt
       61 caataatata ggtattaaca gagacttgtt gctgcgcagc cgcattatta aggcgttcac
      121 tcagcgattc tggatagcgt aacgtaaagg tcttaatctt ttctgtacgc gcatacggct
      181 caatacccgc agctttacaa tcctcaagat actctcgcag ggaaagctct ccttcttttt
      241 gcagcccctg aatactgtca gagacaaaat cacaataacc tgacaagccc aggaatttgc
      301 cacgaaatgc attcagttct ggcacgtagg taataacagc tggttgcccg gcaatctcca
      361 tagagtttgg cgtcttcagt ttgatcatgg ttttacccct atacttagta accagtcaca
      421 cacgctttct acagcgcctt tatcggtatc tggcgacgga tgaggccgat ggaaacacgc
      481 aacgctcata tttagtatga acttgcaacg cgaacctctt ccttccttaa tttctccgcc
      541 taatgcttta accagtgact ctatatcaga ccacttgatc ccttgaggaa caggagtttt
      601 aaatatttga tccaatgtat ttttttgttt cttccgtaaa gcctgtacct tggttctcat
      661 ggcatgtcct tatgacttca ctttatgaca tcacagtatg aagtcaagtt tcccttcctt
      721 acgccattgt cccaatcgtc ttcctgaatc gcagcagtgc gatggtgaaa aacgcgccgc
      781 caattgccat cagcgtcagg aactgcggcc agacgatttc gaatccggca ccccggtaga
      841 ggatggcctg cgcgaggcta acaaagtgtg tcgtcggcat ggtcagcata atgtcctgca
      901 ccatctgcgg catactttcg cgcggcgtgg atccaccgga aagcatttgc agcggcagca
      961 gcaccagaat caccagcagc cccagttgcg gcattgaacg cgctatcgtc cccataaaaa
     1021 tgccgattga cgtggtggca aacagactga gcgccacgcc cagcataaac agcgggatcg
     1081 agccttcaat cggtacgccc agtacacctt tcaccatcag caccagcgat aatcccgata
     1141 ccaccagcac caccagcccc atcgaccaga tcttcgccat catgatctca aacggcgtta
     1201 tcggcatcac cagtaaatgt tccaccgtgc cgtgttcacg ctcgcggatc agcgccgatc
     1261 cggtcaatac aatcgccagc atggtaatgt tgttgatgat cgccatcacc ccgccaaacc
     1321 acgcgggatc aaggttcggg ttaaagcgca tccgggtttc cagcgatacc aacggttcgc
     1381 tgttatcacg gtagcgcgcg acaaagctgt tcacttcacc gttgataata ttctggatat
     1441 acccattgcc ggtaaatgcc tggctcatgc gcgtggcatc gacgttcacc tgaatatccg
     1501 gctggcgtcc ggcgaggaca tcacgctgaa aattaggcgg aatatttatc gcgaaggtat
     1561 agcgtccggc gtccagtccg gcatccatct catcggcggt gatcatctcc ggtggcaaaa
     1621 accacggacg atagaagctg ttaacgatcc ggttcgataa ctgcgattga tccatatcgg
     1681 caatggcgat cggcgcgagg ttcaacgatc ctggcgtaac ggtcgctgac gaatacaccg
     1741 acaccgtaaa cgagaagaca atcagcgtca gcatcgcttt atcaccgagc agactgcgca
     1801 actctttgat acccagatta aaaatattgc gtaaatggcg catcatccct cctgtttttt
     1861 cagcagcagg atgcttaagc ccatcaccag cgggatggct atcagcaacg ggataaaaag
     1921 ttgccacaaa tcagtcagat ccagcgcttt cgagaacgtt ccgcgggcga tagtcagaaa
     1981 atgactggtc gggtaaacct cgccgatcca acgtccaggc ccttccagcg aagctaccgg
     2041 atcgatcatc ccggaaaact gtgtcgccgg gatcaatgtg ataatcgccg ttccgaaaat
     2101 ggcggcaatc tggcttttca taaaggtgga gatcagcagc cccattccag tggcaatgat
     2161 gatatacagc agcgccgcca aggtgagcgt caggaaactg cctttatgcg gtacgccaaa
     2221 cacaaacacc gacaggccgc agagcaggaa aaagttcagc atccccagcg cgatgtacgg
     2281 cagctgtttg ccaagcaaaa attcgctacg ggtggtgggg gtcacgtaaa ggttgataat
     2341 cgaccctagc tctttttccc gcaccacgct aagggcgctt agcattgacg ggatcatcat
     2401 cagcagaagc gggatcaccg ccggaacaat cgctggcagg ctttttacgt ccgggttata
     2461 gcgataacgc gtctcaatgt tcatcagccc gctttggctg gcgggtgtcg attgtcggct
     2521 cgccacatcc tgaagccaac tctggtgcat tgcctgcacg taacctttta ccgtttcggc
     2581 acgactgggc atcgctccgt cgatccagac gccgagttcc acaggcgtac cacgcgcgat
     2641 atcgcgcccg aaattgggcg ggatctcaat ggccaccgtg atatcgcccg cacgcatccg
     2701 acgatcaagc tcgtcataac tggtgagcgg cggctgttcg ataaagtaac gggaaccgga
     2761 gaggttgagt gtccacgcct ggctactgac ggtctggtcg cggtcgagca ccgcaaagcg
     2821 caggttttcc acatccatac tgatgccgta acccattatc agcatcagga tcaccgttcc
     2881 catcagcgcc agcgtcgaac gtactggatc gcgtcgcagt tccagcgctt cgcggcggct
     2941 gtagctaaac agacggcgca ggctaaatcc ctgacgcggc gcgtgggtgg tgtcgtgtac
     3001 cacgggcggc gcttcggctt cgttgctctg ccctgccgct tcctgcaaat aggcgataaa
     3061 tgcctcttcc agactggcgg ctccgcgttt ctcaaccagt tcctgcggtg taccgctggc
     3121 aagcactttt ccggcgtgca tcagtgagat gcggtcgcaa cgttccgctt cgttcataaa
     3181 gtgggtggag atgaagatag tcactttgtc ctggcgcgag agatcgacca tcaactgcca
     3241 gaacatatcc ctcgccaccg gatcgacacc agaagtaggc tcatcgagga ttaacatctc
     3301 cgggcgatga atcaccgcca ccgccagcga aagccgctgg cgaatgccga gcggcaatga
     3361 ctccggcaga acatcttcaa cgtcgttgag cttaaaacgc tcgctcattt cagccactcg
     3421 cgcgggaatt tccgcttccg ggatgtgaaa caaacgggca tgtaactcca ggttttgccg
     3481 cacggtgagt tcgttataga gcgaaaacgc ctgcgacata tagcccacac gacggcgggt
     3541 atcgatatct tttggatcaa ccggttgccc aaatagccac gcctcacctt cgctggcggg
     3601 cagcagtccg gtaagcattt tcatggtggt ggatttaccg cagccgttcg aaccaaggaa
     3661 tccaaaaatc tccccgcgtg gaatgcggaa attgacgtga tcaacggcaa cgaaggaacc
     3721 aaaacgcatg gtcagatcgc gcgcttcgat ggcaatctct gcgttttcag gttgatacgg
     3781 tgggatcact accgcctgat gcgcctggcg ttgcgcttgc ggtaacagat taataaatgc
     3841 ttcttccagc gtggcgcttt gcgtttgctg ccgcagctct tcggcgctgc cggttgccag
     3901 cacttctccg gcattcatcg ctaccagcca gtcgaagcgt tcggcctctt ccatataggc
     3961 ggtggcgacc agcacgctca tattgctctg ccgctggcga atactgtcga tcagatccca
     4021 gaactgggcg cgggagagcg ggtcaacccc cgttgttggc tcatcaagga tcaacagttc
     4081 cgggtcgtgg attaacgcgc agcacagccc aagtttttgc ttcatcccgc cggagagttt
     4141 acctgccgga cgatcgcgaa acggtgctaa cccggtgctg gtcagcagct cattaattcg
     4201 cgcttcccgc tccgctttat cgtgaccgaa caggcgagcg aaaaaatcga cattttcata
     4261 caccgacaag gtgtggtaga ggtttttgcc cagcccctgc ggcatccagg cgatgcgcgg
     4321 gcagacgtct cggcgatgct tcgggtcgtg catatcgcca cccagcacca ccacattgcc
     4381 ctgctcaatg acgcgggcac cggaaatcag tgacaataaa ctcgacttcc cgacgccatc
     4441 cggaccaatc agcccgacca tgcaacgggc ctgaatatcg agagtgatat tgttcagcgc
     4501 aacggttttt ccataatgct ggctcacgcc cgccagttgc gcgacaggcg ggacgggaac
     4561 cagttccaga tgcgtcattg cggcaacctc accacgaggt cgtcaggcca cggaagttct
     4621 tcattcaccc gcacccacgc tacgcccggt aaaccggttt tgacatattc cagatgctgc
     4681 tggagtaatt ccggtgggat acgcgctttg acgcggaaca tcagtttcag ccgttcatcg
     4741 ctggtttcga cggtttttgg cgtgaactgg gcgacactgg cgacaaaact gatggttgca
     4801 ggaatacgca gatctggcgc ggcatcgagg atcagccggg cttcaccgcc cagtttcagc
     4861 gtgcccgcct gttcggttgg caggaagaag gtcatataga cgtcgctgag atcgaccata
     4921 ttcagcaccc gaccgcctgc cgccagcact tcccctggct cggcaacccg atactgcacg
     4981 cgtccgtcac gcggggcttt cagttcactg tcatcgatat ctgcggcaat gcgccgttca
     5041 gtggcttgcg ctgcttcgac ccgagtttgc gcctgaatga tattggtgcg tgctgcttct
     5101 atagccgctt tagaagccga aacctgggct ttcgctgatt ccagcgcagc tcgggcgctc
     5161 tcagcggcgg cgcggtcgtc atccagctgt tgcgcagaaa tagcccctcg ttgggcgagc
     5221 gagcgggaac gcgtatgacg tttggcaaca gagtccagtt cggcctggcg ttgattgacc
     5281 agcgactgtg cggcacgagt ttcactttgt cgttgctcca gcaaagcctg cgcggcggca
     5341 acggcgcttt gtgcctcttt gatttgcgcg atggcttcca gtcgctgttc ctgcaacacg
     5401 cgagtatcca tcttcgccag cacttcgcct tcgcgaacaa actggccttc tttcaccaga
     5461 atggtgtcga tacgcccggc aattttgctg gcgatatcca cttccgtcgc ttcaatgcgc
     5521 ccattgctga cagcaaagcc ttccggcaca cccgccgggc gcaacagcca ccacgccacg
     5581 acggcagcca ccgccagtgc cccgacaacc caccacgcca gatggcgctt actcttatcc
     5641 ataatcgacc cgccataatc cctgtaagca aacgacgcgt gttacaactg ccgccgaacg
     5701 acgttcagcg gattcttcca tccgggataa gaaggaaacg acaaatacga ccagcgcgca
     5761 gagcattgag cgccaacggg gaaggcttag tgaaaacggg tagtaatgtt gtcatcatca
     5821 gcattcctgg ccgtaaatga aacacgcata ctcataagtg tgatctataa aactggctga
     5881 caaaacggta tccatcctgt tggatcgcat ttcacgcctg ttcagttgca ggaacagagc
     5941 attccttttt acgttttatc aacagtctca accagatagt aacattaata ttcatgttgt
     6001 ctatggttca gagctgtaac attgtgcgcc ttctaatcaa aggcataagt ccatttctgt
     6061 ttttttcatg ctggtaagac ttccagggag tggttatctc tggccctgca ccagggttaa
     6121 tttggcaatt atgcatgctg aacattcttc cagcatgttt actgtcacgg gcgttaagac
     6181 ctaaaactgt ccccgtttca cctatgcgtt ctctcccacc atttccgtta tgtgcaacaa
     6241 cttgatttat acctaacatt tgtgaacatg ctaatagtgc ttcaagttta ggtctgtaat
     6301 ctgtcgaaac agaaaaataa cgattaaagg atcgacaaga gatagcccgg ctagttttaa
     6361 actttttgcc attaattata gcatgatgct ttttattaat aatttccgct aattcgaccg
     6421 gatcgagagc attggtcatt tcatcaatag agacagttat cggtcctata atgtaacgtt
     6481 ttcgctgatc atcaaaagtt atcccatgat gatttgccat tattttgtat ttggggtcat
     6541 agaaacatac aggataatct tttatcccgt cgtaggtatc ctcatcaagt ggtttcatgc
     6601 gcgtgaagtt ttgcatatag ttaccattgc aattcgtttc atgatttcca gctaatacat
     6661 gaacattttc attgatactc attatgcgtt ttaacaaatc gatgataaat tgatcgccac
     6721 agatattact aaatcgatcg ccggtttggt cgccaagaaa cgtacatggc gtgactgcgt
     6781 ctttattaat aaccaaataa ggctgaagac tttctaaaac attatctctt ctgttcagta
     6841 tactgattat tgtattaaca ttagtgtcaa gttgctcatt aaagttctcc cttagcaata
     6901 cgttgagaac ttcatcttct atattaagca actggctaag gaaattttct ccttcagcca
     6961 tgattcccaa atgtcctgtt gcaaaaagcg cgtacaaaac tgcaccgact gagccatcgg
     7021 tatcgccaaa ataggtcggc ctggtacttg tgggatcaac aggtgaacaa ttggagaaat
     7081 aggttctgtc agcgaactgg ggttttataa ttgtttcgaa tttaatatca aatacattgt
     7141 attgcgatat ttcccagttc cagatgggtt tgtacgactc agtaactgca acatcattag
     7201 cattatcatt tactgtcata ttattaagta aataaacaat gctgcaccaa ttaatatcgg
     7261 tattatcaag agggcaaccg taatttttac tattaattgt cacaataaac tgataacagt
     7321 tattcctacg tagaatatga gcgcgcatgc ccgatagcat actatatttt tctgaatgat
     7381 tcgtttgtat ggtaaatgaa attttgtcat cttgaataga ttgttggaaa cgttctattt
     7441 gccaatcatc aattttttcc acaacttcac cgagcattgt cctggcaata cttgatatat
     7501 catcacctat accatttgca atatacccaa acagttcttt agtgaagttg gtaacatgct
     7561 cttgcaccat atcatttgtt gcgattgtcg ttgtactgcc attagtgcct gccacagttc
     7621 caattttcat cttttacctc atattattca ccgcgaggaa tacatagtta acattttatc
     7681 ttatgacata cacctcatat tcgatgggga aataattata ttttgcacaa atcattcagc
     7741 cttaaagcat aatatgaaat ttcttatctc atagcagaga aagtccctaa atattgtaga
     7801 ggcgtcatca gtacatacct ctacaatcga ttaagaaaac ctatgaaata ctggcaaaat
     7861 taaaaagaga taccacagca ctcatgaagt tttattgagg ttgaaaacaa ctaagccctg
     7921 ggacaccttt tttatcacca cgttcgttag gtgctatctt ccaagaattt agccccatgg
     7981 tataattatc aattttttga tgctggtcta cccctaatcc ataatggcca aatttctgtt
     8041 tagttttttt gagcgtctgt ccacatttga agatatcttc aggttttggt ctgaaagcag
     8101 tagtgttatt aaaataattt tcatatacag gtgtgggtgc tatagcattg cttctaatta
     8161 gatggagaag atgatcatcc atgtgttgtt tatgtttttt attacatata tttgctaatt
     8221 cctcaggatc aattgggtct ttcatctgat taaatggaac ctgcaaagca ccgagacaat
     8281 aacattgatt ttcttcattt cttattattc cgtgatgact ggttaaaact ttagtttctg
     8341 agttataatt acaaacatcg agcgttttaa ttaagttata cgtatcaccg gcagagagtt
     8401 tataattttc tagtcgtgca gtatagttac cattaaaatt gatttcatga tttccagcca
     8461 gaacgatgac atttttatta atacggctat ctttattacc ctccatattt cgcatagaat
     8521 ttagcaaagt aaggatatat ttatcaccaa aaatagtact aaatctatcg cctgtatgat
     8581 ccccaaggaa gatacaaggg gttatcgcat cgtcctttaa aaaaacatgt tgatgcaact
     8641 cattcaatat catgtctctc gtatttaata aattgtagag caatttatta tttttatgta
     8701 tgtatgcaga tgaagccatg tcttcgtgtt ttaataattc acataataaa ctccaaccct
     8761 tttccctgat gcctatatgc cctgaaacga gcaatgaata aagtacagcg cctactcttc
     8821 catctgtatc gccaaaataa gccgtgttgg cattttgtgg gttaatcaca ccacattgtt
     8881 tataaaagtc tattttagaa aacacagatt taacatagct atcaaactta tccttaggaa
     8941 acgtctcttt agagacatcc caacgccagt cagctttaaa attaccgcta agaagagtaa
     9001 agaaattttc aggtatgata gaaagcccat taactttata aattatgtct gatttatctc
     9061 cggagaaatt aataaaataa taaccatcct tacactcaat cttaactgta atgggagacg
     9121 ataattttat cacatggtta ttgtctggta taattgtaaa gataaacgtg ttagtagtat
     9181 tatcggcaaa actattatat tcttgaatat ctatagatgg ctcactattg taaatccata
     9241 ttttctccat ctgaagacta tgttcattta ccctccagtg accatgttgc aggaaataga
     9301 tgatatgcat gccaaaatcg ttatctattt gcgaatcatt atccagctta tcaatttcaa
     9361 ctatctttga acgatgtgtt ttatcaacct tgctatggtg ttgatgatta ttaactttat
     9421 ctgaatgctt atctttatgg tttgaaggga tgtacttaaa tatattgtca attgctttca
     9481 taaataaccc gcgatatttt tgacctgtta gtaaaaaata aatgatacat gtctgaaaga
     9541 catatttacg acgatataaa acaataatac ttatatgttc gctgatttta agcaccgaga
     9601 ctatccggt
//
LOCUS       DAZXUL010000143         9598 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZXUL010000143 DAZXUL010000000
VERSION     DAZXUL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.143"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            25..1605
                     /locus_tag="ACH3L1_003625"
                     /pseudo
     CDS             25..1605
                     /locus_tag="ACH3L1_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            1828..2325
                     /gene="ubiC"
                     /locus_tag="ACH3L1_003626"
     CDS             1828..2325
                     /gene="ubiC"
                     /locus_tag="ACH3L1_003626"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFT4078214.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            2338..3210
                     /gene="ubiA"
                     /locus_tag="ACH3L1_003627"
     CDS             2338..3210
                     /gene="ubiA"
                     /locus_tag="ACH3L1_003627"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFT4078215.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(3365..5788)
                     /gene="plsB"
                     /locus_tag="ACH3L1_003628"
     CDS             complement(3365..5788)
                     /gene="plsB"
                     /locus_tag="ACH3L1_003628"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFT4078216.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5959..6327
                     /gene="dgkA"
                     /locus_tag="ACH3L1_003629"
     CDS             5959..6327
                     /gene="dgkA"
                     /locus_tag="ACH3L1_003629"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFT4078217.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            6437..7045
                     /gene="lexA"
                     /locus_tag="ACH3L1_003630"
     CDS             6437..7045
                     /gene="lexA"
                     /locus_tag="ACH3L1_003630"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFT4078218.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            7118..8443
                     /gene="dinF"
                     /locus_tag="ACH3L1_003631"
     CDS             7118..8443
                     /gene="dinF"
                     /locus_tag="ACH3L1_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFT4078219.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            8559..8768
                     /locus_tag="ACH3L1_003632"
     CDS             8559..8768
                     /locus_tag="ACH3L1_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFT4078220.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(8810..9325)
                     /gene="zur"
                     /locus_tag="ACH3L1_003633"
     CDS             complement(8810..9325)
                     /gene="zur"
                     /locus_tag="ACH3L1_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFT4078221.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         2345 a         2184 c         2481 g         2588 t
ORIGIN      
        1 ttatttctgt attacagagg ttttatgata ggtcatatca caaattctgg cggcaataac
       61 tctttgatga aacatgatgt ggtgcaagga aataatatag tagatcttga tttactacgt
      121 aatttcaatg gggtgacagg tttaaacaga gattacttta tttatatcag tgatatattt
      181 ttaaatataa aacaacggaa cgaaaaaaat catgcaataa atatgttacg tgaagtctca
      241 attagtaatg atactataag tgtaaaattc tacagaaatg aaaaaataga atgcgcttgt
      301 aattttctga tggataaaga tgcgcagggg tatatcgacc tgtctgattt tgatttaaca
      361 aattgtcatt ttaaaggtga cgttatttct aaggtgtctt ttttatcatc aaatctacaa
      421 catgtaactt tcgaatgtaa agaaattgag aattgcaatt ttactaaggc aacagttgat
      481 aatgtcatat ttaaatgtcg acgtttacac aatgtgattt ttctcaaaac gagtggcgaa
      541 tgtgttgatt ttagccaaaa tattcttgat acagttgact tctcgcagag tcaacttggt
      601 catagtaatt ttcgcgaatg tcagattaga aattcaaact tcgataattg ttatctttac
      661 gcttcgcact tcaccagagc agagtttctg tctgccaaag aaatatcatt tattaaatcg
      721 aatttgacag ctgttatgtt tgatcatgtg cgaatgtcga cagggaattt ttaagattgc
      781 attacagaac aattggaatt aactattgat tattcagata tatttgggaa tgaagatctc
      841 gatggttata tcaataacat tataaaaatg attgatacat tgccagataa tgcaatgata
      901 ttgaaatccg ttctggcagt aaaactggtg atgcaattaa aaattctcaa tattgttaat
      961 aaaaacttta ttgagaatat gaagaaaaca tttagccatt gtccttatat aaaagatcca
     1021 attatacgta gttatataca ttctggggaa gatgacaagt tcgataattt tatgcgtcaa
     1081 catcgattca gtaaggtgaa tttcgatacc caacagatga tccattttat taacagattt
     1141 aatatgaata aagggctggt tgataaaaat aacaattttt ttatccaact tatcgatcag
     1201 gccttacgat caacggatga tatgatcaaa gcaaatgcct ggtatcttta taaagagtgg
     1261 attcgtagtg atgatgtttc acctatattt atagaaactg aagaaaagtt aagaaccttt
     1321 aacactaata aattaacacg aaacgataat atatttatcc tgttctcctc tgtcgatgat
     1381 gggcctgtta tggtggtaag ctcccagcgc ttacatgata tgttgaatcc tacaaaagat
     1441 accaattgga attccacgtg tatctataaa tccagacata agatgttgcc tattaatctt
     1501 actcaggaaa cacttttcag ctccaaatct catggtaaat atgcgctttt cccaattttt
     1561 actgcgagtt ggcgagctac tcgtataaag aataaaggta tttaagtaaa ggaaaacatc
     1621 accgttccta gcatcatggg cggtgatgcc cctatggttg ccctcgccag cacgggcatc
     1681 ggcaaagcgt aaggttcaac agcgttttac cacttcatgc gattgttgcg tttttgttgc
     1741 gcattagatc acttaatttg ctttacatct cccgtaaaca cttttctgcg atacaatgcc
     1801 tttacgttat gtaacggaga gttcggcatg tcacaccccg cgttaacgca actgcgtgcg
     1861 ctgcgctatt ttaaagagat ccctgccctg gatccgcaac tgctcgactg gctgttgctg
     1921 gaggattcca tgacaaaacg ttttgaacag cagggaaaaa cggtgagtgt gacgatgatc
     1981 cgcgaggggt ttgtcgagca gaatgaaatc cccgaagaac tgccgctgct gccgaaagag
     2041 tctcgttact ggttacgtga aattttgtta tgtgccgatg gtgaaccgtg gcttgccggt
     2101 cgtaccgtcg ttcctgtatc aacattaagc gggccggaac tagcgttaca aaaattgggt
     2161 aaaacgccgt taggacgcta tctgttcaca tcatcgacac taacccggga ttttatcgag
     2221 ataggccgtg atgccggact gtgggggcga cgttcccgcc tgcgattaag cggtaaaccg
     2281 ctgttgctaa cagaactgtt tttaccggcg tcaccgttgt actaagagga aaaaaatatg
     2341 gagtggagtc tgacgcagaa taagctgctg gcgtttcatc gcttaatgcg tacggataag
     2401 ccaattggcg cgttactgct gctctggcca acattatggg cgttgtgggt ggcgacaccg
     2461 ggcgttcctc agctctggat catggcggtg tttgtcgcgg gtgtctggct gatgcgcgct
     2521 gctggttgcg tggtgaatga ttatgctgat cgcaagtttg atggtcatgt taagcgcacg
     2581 gcgaaccgac cacttcccag cggcgcggta acagagaaag aggcgcgcgc gctgtttgta
     2641 gtgctggtac tgatttcgtt tttactggtt ctgacgctga atacgatgac cattctgttg
     2701 tcgattgccg ctctggcatt ggcgtgggtg tacccgttta tgaagcggta tacccatctc
     2761 ccgcaagtgg tgctgggcgc ggcgtttggc tggtcgattc caatggcttt tgccgctgtg
     2821 agtgagtcgg tgccattgag ttgctggtta atgttcctcg ccaatattct ctgggcggtg
     2881 gcttacgaca cgcagtatgc gatggttgac cgcgatgatg atgtgaagat tggcattaaa
     2941 tccacggcaa tcctgttcgg ccaatacgat aaattgatta ttggtatttt gcagattggc
     3001 gtactggcac tgatggcgat catcggtgag ttaaatggct taggctgggg atattactgg
     3061 tcaattgtgg tggctggcgc gctgtttgtt tatcaacaaa aactgattgc caaccgcgag
     3121 cgtgaagcct gctttaaagc atttatgaat aataactatg ttggtctggt actgttttta
     3181 ggactggcaa tgagttactg gcatttctga tgatgtaaaa aagccggatg atcatccggc
     3241 tttcttctgg gttgcctgat gcgttgcgct tctcaggcct acccggtgaa tcgcaattta
     3301 ttgaatttgc aggtcatggt aggccggata aggcgttttc gccgcatccg gcaattctct
     3361 ctgattaccc ttcgccctgc gtcgcactct caatcgtcaa acgcacgtct gatgtaatca
     3421 actccgccag caactgataa accttcatcg tttctgctgg ttcggcatcg ccgctatcgc
     3481 tgatataccc ttcatcacgc agcgtcagca ccagagaact gaacaccgcc ttgtcgaaga
     3541 actccggcgc gttgatgccg tgcagcacgg agagacgttg cgcaacggtg cggctctctt
     3601 tctccagcgt accgcggttg atcgacgggt tggcactcaa caaccagaag gtgatggcat
     3661 aacgttgcag cgtttcgcgc gcgcctgcgg ccagcagctg tagggtgcga gaatgcgccg
     3721 ggttgatatg caactcatca tcttgcaggg taatcagccc ctgacgttgc atctcatttg
     3781 ccagcgcatc aataacgtcc ggcaactcgt cgcgatccca gcgcaggaac agctccgctt
     3841 tcagcattgg gtaaagcaca ttgacgtgct ccatcaatac gtcgcgggag atgtggcgat
     3901 gctgggtgac aattgccgcc atcagcgaag gcagcaccaa catatgcgca atgttgttgc
     3961 gatagtaggt catcagcacc gcttgctcgc gtggcagaat gatgatgtcg ccgatagtgt
     4021 ctttctcgac ttcaaacttg ttcatttgca gcgcgtgatc gataagctcg ctggcgctgg
     4081 ctgaaggaac ggtagagtcc gtggagtaag gtacgttgcg catcagatcc aggtagcagt
     4141 tgagttgctc ggttaactgc tcgcgggtga gtgagcgctg acgtgatgcc agtagcgcag
     4201 tacagcacag gttcatggcg tttgccgcgc ctgcgttgtt aatgcgtacc atcagatcgg
     4261 cagcaatatt attgaccgtc ggcgttaacc aggccggacg caccgcttcg atgggatcga
     4321 tagattcacg ccagtctggt acgtgctggt taaggtaggt catcaacggc attggttcac
     4381 cgaagttgac gtaaccctga ccgagattac gcagcttgct taaaccgcgc agcatctgcg
     4441 gcaggctctc tttctctttc gtcgcgccgc gcagttcttt ggcgtaagta cccacttcca
     4501 tgacgtgctc ataaccgata tagatcggaa tcagcgtaat cggacgcgtg ccgccacgca
     4561 gcatcgcctg aatggtcatc gacagcgtac cggttttcgg atccagcaaa cgccccgtgc
     4621 gggaacgacc gccttccacg aagtactcga cggaataacc acggctgaac agttcgccga
     4681 gatactcacg gaaaacggtg gaataaagtt tattgccttt aaaagtacgg cgaataaaga
     4741 acgcccccag acggcggaaa atcggcccgg ccggccagaa gttcaggttg atcccggcgg
     4801 cgatatgcgg cggcaccagt ccctggtggt acagcacgta agaaagtagc aggtagtcca
     4861 tgtgactgcg gtggcaaggc acatacacca gctcatgacc gtcgtgggcc agctggcgaa
     4921 cgcgctcggc gttatggacg ttgatgccct gataaaatcg gttccaggtg aagcccagaa
     4981 tgcggtcagt caggcggatc atctcgtaag agaaattcgc cgcaatctct tccatcagcg
     5041 caatcgcgtt ctgctgcgct ttttcatggg agattttttt gctgcgcgct tcatcttcta
     5101 ccgctttggc aatggcgcgg gaggcgagca gcttattaaa cagatcctga cgagcaggca
     5161 gacgtgggcc tacggcagcc agacgttgac gggcaaagtg catacgcgcc acgcgcgcca
     5221 gtttctgagc gatagtttta tccgtgccgt gttcatccgc catacggcgc agcgacactg
     5281 acggcgagaa acgcacaaaa ctgtcgcgac cgagccacag tacagcgaaa aatttctgta
     5341 cgccgttaag catacgcagc ggcgggttca cttcgccttt ttcacgcccc ggcgcgcgac
     5401 caaacatcac cgatactggc accatctgca catccagatt tgggttgcta cggtgcaaat
     5461 cgagatagtc gtggaaaagc ttaatagact cttctttcgg cgtgtaatag gtgaatacac
     5521 gcggcccgcc gtgaatgaac acatagcgcg gcagtagcgt gccgtcgatt tccagcggct
     5581 ctaacgggtc aggcaaatca tgtgccagac actgggcgcg caacgtcagc aaatctgctt
     5641 tcgagttgta cggtaaaacg tacataattg gacgagaggt atccagcccc agttccgggg
     5701 caggatctgc cggaatagac ttgcttttta ccaggatgct taatggtaaa ttcagtaatt
     5761 tgtagtaaat tcgtggccag ccggacataa acgatgtaaa gcctctggtt aataatgcaa
     5821 atgcgcggca aggatagcag aaagtcatgg gaaattctgt ggtatccgct catgtttcgc
     5881 gcggcgttac gcaaacccga atcatcggat ttaacggtac actgatattg acgctcataa
     5941 tgtaaaaagg ttctttcaat ggccaataat acaactggat tcacccgaat tatcaaagct
     6001 gctggctatt cctggaaagg tttacgcgct gcatggatca acgaagcggc attccgtcag
     6061 gaaggcgtag cggtattgtt ggcggtggtc atcgcctgct ggctggatgt ggacgcaatt
     6121 acccgcgtgc tgcttatcag ctccgtgatg ctggtgatga ttgtggaaat cctcaatagc
     6181 gccatcgaag cagtggttga ccgaattggc tctgaatacc atgagctttc cggacgcgca
     6241 aaagatatgg gatccgccgc ggtgctgatt gccattatcg tcgccgtgat tacctggtgc
     6301 atcctgttat ggtcgcattt tggataaccc ttccagattt cgataaatct ctggtttatt
     6361 gtgcagttta tggttccaaa atcgcctttt gctgtatata ctcacagtat aactgtatat
     6421 acacccaggg ggcggaatga aagcgttaac ggccaggcaa caagaggtgt ttgatctcat
     6481 ccgtgatcac atcagccaga caggtatgcc gccgacgcgt gcggaaatcg cgcagcgttt
     6541 ggggttccgt tccccaaacg cggctgaaga acatctgaag gcgctggcac gcaaaggcgt
     6601 tattgaaatt gtttccggcg catcacgcgg gattcgtctg ttgcaggaag aggaagaagg
     6661 gttgccgctg gtaggtcgtg tggctgccgg tgaaccgctt ctggcgcaac agcatattga
     6721 aggtcattat caggtcgatc cttccttgtt caagccgaat gctgatttcc tgctgcgcgt
     6781 cagcgggatg tcgatgaaag atatcggcat tatggatggc gacttgctgg cagtgcataa
     6841 aactcaggat gtacgtaacg gtcaggtcgt tgtcgcacgt attgatgacg aagttaccgt
     6901 taagcgcctg aaaaaacagg gcaataaagt cgaactgttg ccagaaaata gcgagtttaa
     6961 accaattgtc gttgaccttc gtcagcagag cttcaccatt gaagggctgg cggttggggt
     7021 tattcgcaac ggcgactggc tgtaacatat ctctgagacc gcgatgccgc ttggcgtcgc
     7081 ggtttgtttt tcatctctct tcatcaggct tgtctgcatg gcattcctca cttcatctga
     7141 taaagcactc tggcatctcg ccttacccat gattttctcc aatatcaccg ttccgttgct
     7201 gggactggtc gatacggcgg taattggtca tcttgatagt ccggtttatt tgggcggcgt
     7261 ggcggttggt gcaacggcga ccagctttct ctttatgctg ttgctgtttt tacgcatgag
     7321 caccaccggg ctgactgcgc aggcttatgg tgccaaaaat cctcaggcat tagcccgtgc
     7381 gctggtgcaa ccgttgctgt tggcgttggg ggccggggcg ttaattgcgc tgttgcgtac
     7441 gccgattatc gatctggcgc tgcatattgt tggcggtagt gaagcggtcc tggaacaggc
     7501 gcggcgcttt cttgaaatcc gctggttaag cgctccggcg tcgctggcga atctggtatt
     7561 actcggttgg ttactcggcg tgcaatatgc ccgtgcgcca gtaattttgt tagtggtcgg
     7621 caatatcctc aacattgtgc tggatgtctg gctggtgatg gggctgcata tgaacgtgca
     7681 gggcgcggcg ctggcgacgg ttattgcgga atatgcaaca ttgctgattg gtctgctaat
     7741 ggtgcgtaaa atcctcaaac tacgcggaat ttccggcgaa atgctgaaaa ctgcctggcg
     7801 aggaaacttc cgtcgcttgc tggcgcttaa ccgcgatatc atgctgcgtt cgctgttgtt
     7861 gcaactctgt ttcggcgcga tcaccgtact tggtgcgcga ctggggagtg acattatcgc
     7921 tgttaacgcg gttctgatga cgctactcac ctttaccgcc tatgcgcttg atggttttgc
     7981 ctacgcggtt gaagcgcact ccggtcaggc atacggtgcg cgcgacggta gccagttgct
     8041 ggatgtatgg cgggcagcgt gccgccagtc ggggattgta gcgttactat tttcggtggt
     8101 ttatttgctg gcaggggaac acatcattgc gttgctgacg tcgttaaccc agattcagca
     8161 gctggctgac cgctatctta tctggcaggt gattttgccg ttggttggcg tctggtgtta
     8221 tctgctggac ggcatgttta taggcgcaac gcgtgccgcc gaaatgcgta acagtatggc
     8281 ggtggccgcc gcaggttttg cgctgacgct ccttacgctg ccgtggctgg gtaatcatgg
     8341 tttgtggctg gcattaaccg tctttctggc gttgcgcggg ctttctctgg cggctatctg
     8401 gcggcgtcac tggcgcaacg gtacctggtt tgccgcaacg tgacggttaa aaattctgaa
     8461 taaattatcc taagccaaat tgctgactac acttaatctc acgttcagaa gaaaagtgaa
     8521 cgtactctca ttcacaacct aacgatgagg tcttgattat gaataaagat gaagccggcg
     8581 gtaactggaa acagtttaaa ggtaaagtga aagagcaatg gggcaaactg accgatgatg
     8641 atatgacgat cattgaaggt aaacgtgatc aactggtcgg taaaatccag gaacgttatg
     8701 gttatcagaa agatcaggca gaaaaagagg tcgtggattg ggaaacccgc aatgaatatc
     8761 gctggtaatt aatccctcct gcccgacgtg tacaaggatg tacgccctct taacgcggtt
     8821 tctttttcac ctgcacagag tgatcatgct ggcactgttc aggatgacga cacgcttcca
     8881 cttccacgca tgccgcacag agcccatgtg cttcaatcac attatgccgc agggcaaacc
     8941 ccatttttgc cgccagcgta tgcataatgt cttccacgcc ttctgcacac tcttctttca
     9001 ccgcgccgca gcgatcgcaa ataaacatgg ctgacgtatg cgtgggctgg tcgaacagat
     9061 gacagagcac ataactgttg gtggattcca ccttatgcac aaaaccttgc tcaagcagaa
     9121 aatccagcgc gcgataaacc gttggcggct tggcttgcgg ttcagcttcg cgcagtaaat
     9181 caagcagatc ataagcgctg atagcgccat cctgcagact catcaggcgc aacacttcca
     9241 ggcgctgtgg ggtcaggcgc acattacgct gcgcgcagat tttttcagcc tgcgctaata
     9301 actcctgcgt tgtggtcttt tccatcgggc acctcaaagt gggggttaag aatgtcctta
     9361 ctttaccatg ttccaggaaa aacaccgaga acccttattg ttgccgtaat gttgattttc
     9421 tgttttgtag gtaaggtgtt gtgttgcctt atcgtaccat tatcaacacg ataataatta
     9481 ataattcatt tttaaataaa ttgcttcaag ttaactcgct atttatttaa ctgggtttaa
     9541 acgcatacct gtagttcaga aattatgagc gatttttgat agtgaccaga tggcaaaa
//
LOCUS       DAZXUL010000144         9572 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZXUL010000144 DAZXUL010000000
VERSION     DAZXUL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9572
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.144"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..133
                     /locus_tag="ACH3L1_003634"
     CDS             <1..133
                     /locus_tag="ACH3L1_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFT4078222.1"
                     /translation="SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            230..382
                     /gene="hokE"
                     /locus_tag="ACH3L1_003635"
     CDS             230..382
                     /gene="hokE"
                     /locus_tag="ACH3L1_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFT4078223.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            733..885
                     /gene="hokE"
                     /locus_tag="ACH3L1_003636"
     CDS             733..885
                     /gene="hokE"
                     /locus_tag="ACH3L1_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFT4078224.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1007..1627)
                     /gene="entD"
                     /locus_tag="ACH3L1_003637"
     CDS             complement(1007..1627)
                     /gene="entD"
                     /locus_tag="ACH3L1_003637"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFT4078225.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1802..4042)
                     /gene="fepA"
                     /locus_tag="ACH3L1_003638"
     CDS             complement(1802..4042)
                     /gene="fepA"
                     /locus_tag="ACH3L1_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFT4078226.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4285..5487
                     /gene="fes"
                     /locus_tag="ACH3L1_003639"
     CDS             4285..5487
                     /gene="fes"
                     /locus_tag="ACH3L1_003639"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFT4078227.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5490..5708
                     /gene="ybdZ"
                     /locus_tag="ACH3L1_003640"
     CDS             5490..5708
                     /gene="ybdZ"
                     /locus_tag="ACH3L1_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFT4078228.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5705..9550
                     /gene="entF"
                     /locus_tag="ACH3L1_003641"
     CDS             5705..9550
                     /gene="entF"
                     /locus_tag="ACH3L1_003641"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFT4078229.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2105 a         2505 c         2630 g         2332 t
ORIGIN      
        1 cagtaatata agcgaactga aggatgctgt tacggaatat attgaatact ataacagcag
       61 aagaattagc ctgaaattaa aaggtctgac tccaattgaa tatcggaatc agacctatat
      121 gcctcgtgta taactgtcca actttttggg gtcagtacac cctggggctg ccactccaaa
      181 ccagacaatt tggatggtag tcccttcttc gcatggaggt aatataaaca tgctgacgaa
      241 atatgccctt gtggcagtca tagtgctgtg tttgacggta ctgggattta cgcttctggc
      301 cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat attgagttca gggctgttct
      361 cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa tccccgcata tccggttgtc
      421 aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg cggtcagtga tggctgcggg
      481 cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga agtccgaaac aggactcact
      541 gtataaataa acagctattt tgctaaggaa gggtaagata acggcgggtg cctgaagctt
      601 tccggtttca ggttaactct gaggtttgga aagatgaagc cccaagagat atttctatca
      661 atcctggggc tgccactccg aacctcaaca actcggatgg tagtcccttc ttcgcatgga
      721 ggcaatataa acatgctgac gaaatatgcc cttgtggcag tcatagtgct gtgtttgacg
      781 gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg agttcaccgt aaaggaacgt
      841 aatattgagt ttaaggctgt tcttgcttac gaaccgaaga agtagccgtt gtgcggggag
      901 taatccccgc atatccggtt gtcaggtcag agtggtaagg cacctgcttt acactttcgc
      961 ccgcagtcag tgatggctgc gggcgaatcg taccagatgt tgtcaattaa tcgtgttggc
     1021 acagagttat gactattttt tcttttatct gccagtgcac agcaaacatc tcattctcac
     1081 gatgaatgat gacctgctgt ttattccagc taattatctg atagtccaga aaacctgcat
     1141 cagtttggat ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgca agtgtcagcg
     1201 ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg ttctgctggt gtaataatgt
     1261 tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc ttcgatatca acgccaatcg
     1321 gttgacggga aaccacggcc aatgccgtag ccccgcagtg gctaatactg ccgtatacct
     1381 ccgcaggcca caccggttgg cgtagctcgc cgattgcggg cacacattta tagccatatt
     1441 cccgcaaggc ataaacagca gcgatccgtc cggctaaatg ctctgtttta cgtttacgtc
     1501 cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa atcctgctca caaaaattcg
     1561 ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag gtatgcgtag
     1621 ttttcatatc gaccatattc gagactgatg acaaacgcaa aactgcctga tgcgctacgc
     1681 ttatcaggcc tacgtgatcc tgcaatatat tgaattggca agatctttgt aggccggata
     1741 aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt gtcaacaatc tgacgttagc
     1801 atcagaagtg agtattaatg ctcatatacc acgtacgtcc cggctcgtta taggtatacg
     1861 cgccggcacc ggcgatatag ttggctcctg ccagatcgcc cgtggtctgg gcattacctg
     1921 cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc ggtcagactg acattcttcg
     1981 tcacatccca ggtcgcgctc agacccacaa tgctgtaagg actgatttct ttggtttccg
     2041 ggccaaccgc tggctgacct ttatagttgt acttcttcgg ctgctgcttg ccgtaccagg
     2101 tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca gctcagcgtt gagttcaacg
     2161 tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt gttttcactc ttcagcatat
     2221 aagtgatgtt attggtccac atcaccgttt cgctaactgg tacgtttaac gatccttcca
     2281 gaccttcaac cactgctttc ggcacgttat cccactgata gagatcagtg ccgactgcgt
     2341 tttgccctac agccacatag cccgcttcaa tcttattgcg ataatcgtta cggaaccagg
     2401 tgacgcccgc cagccagccg tcgcgtttga actccagacc aatctctttg ttgatgctgg
     2461 tttctacttt caggtcatcg ttaccttgca gatagcagcc gcccgcgctg gcatagcagc
     2521 cctgaccttt actgtagaga atgtagttcg ggttagtctg gtataggctc ggcgctttat
     2581 aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc taaaccttgc gatatgttca
     2641 gcgccgggct ccagttattg ccgacaatac tgtgatgatc gaaacgcagc cccggcgtta
     2701 cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa cagcgagaaa atttctgctt
     2761 ttgaatacgg gctacggtcg gtggcactca cgccatcaat agcgccaccg gtattcgttc
     2821 cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg ttgctgattc cactccgtac
     2881 ccagcgtcag cgtctggtta acgaggaaat caatcggcag gttaacttcg ctgtgcagca
     2941 tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc tttttcgtta aatttccctt
     3001 cggtaccgcc cgccagacct tccggaatac gcgagttacg ggtgtgttca tactgcaccc
     3061 agttgctggt ggtcacgccg ttatcccagc caccgttcca ggtcagcgcg tagttctggc
     3121 gatacaggcg gttggtttca tcgccatatt tcgagcgagt gtaagcgtca gagttggtgt
     3181 tctgggtatc gcccgcatac aggttaccct ggcggctgta acccgcttcc agttccagcg
     3241 attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat atctttattg atcacccctt
     3301 cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg cgcggactga tggccctggt
     3361 tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc atacaagcgg aagctgaatt
     3421 cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt ggtggcacct tcctctttat
     3481 gttctggcgc attgaaatat gcgtcccagg agccgtgcca ctcgccgctg ccttttttgg
     3541 taatgatgtt aaccacgccg cccgccgcgc cgttgccata acgcgcagct gccggaccac
     3601 gcagaacttc aatacgttca atcatttcag gtggcaccca ggaggtatca ccacgggtat
     3661 cgcgctcgcc acgccagccc tgacgcaccg agttacggct gcttaccggc ttgccgtcaa
     3721 tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc aatctgtcgg ttattcccac
     3781 gctgaccact ggtggagtta ccggtcaggt taacgcctgg catggtacgg atgatctccg
     3841 acacatcgcg ggcaaccggg tttttgcgga tttcatctgc ggtgatggtc gaaacgccag
     3901 gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt atcgtcatgt gaaacaggtg
     3961 tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc cagattgacc aacaaggcca
     4021 gggaatgaat cttcttgttc attgttttat tcctgcattt ttgccacgaa ttgcaactgt
     4081 cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc gaaaaccttt gatcctgaaa
     4141 gacacgcagt gcagttggtt aattaatgtc cgcgcttccc acggcgcgcc attacgctat
     4201 tgcaaatgca aatagttatt aataacatta tcaatatatt tctgcaatca atgaaaaatt
     4261 gcacagtaaa catggggtta tggtgtgacg gcgttaaaag taggaagtga gagctggtgg
     4321 cagtcgaaac atggcccgga atggcagcgt ctgaatgacg aaatgtttga ggtcactttc
     4381 tggtggcgtg atccccaagg ttctgaagaa tactcgacga taaagcgcgt atgggtctac
     4441 atcactggtg tgaccgatca tcatcagaac agccagcccc agtcgatgca gcgaattgca
     4501 ggtactgatg tctggcagtg gacgacacaa ctcaatgcca actggcgcgg cagctactgc
     4561 tttattccca ccgaacgcga tgacattttt tctgcaccat cccccgatcg cctcgaatta
     4621 cgtgaaggct ggcgaaaact attaccccag gcaatagccg atccgctgaa tccacaaagc
     4681 tggaaaggcg ggctagggca cgctgtttct gcactcgaaa tgccgcaagc gcctctgcaa
     4741 ccgggatggg attgtccgca agcgccagaa ataccagcca aagaaattat ctggaaaagt
     4801 gaacggttga aaaattcacg tcgtgtatgg atttttacca ccggcgatgt aacagcagaa
     4861 gaacgcccgc tggcagtttt gctcgatggc gaattttggg cgcaaagtat gcccgtctgg
     4921 cctgtgctga cttcgctgac ccatcgtcag caacttcctc ccgccgtgta tgtgttgatc
     4981 gacgctatcg acaccacgca ccgcgcccac gaactgccgt gtaatgcgga tttctggctg
     5041 gcagtacagc aagagttatt acccctggtg aaagttattg ccccttttag cgatcgcgcc
     5101 gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt ccgcgctgta tgccggactg
     5161 cactggcctg aacgctttgg ctgtgtatta agccagtcag gatcgtactg gtggccgcat
     5221 cggggcgggc agcaagaggg cgtgttactt gaaaagctaa aagctggtga agttagcgcc
     5281 gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc cgatgatcat gcgggccaat
     5341 caggcgctgt atgcgcaatt acaccctata aaagaatcca ttttctggcg tcaggttgac
     5401 ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc aggggttaat cgacctctgg
     5461 caaccacttt tccatgacag gagttgaata tggcattcag taatcccttc gatgatccac
     5521 agggagcgtt ttacattttg cgcaatgcgc aggggcaatt cagtctgtgg ccgcaacaat
     5581 gcgccttacc ggcaggctgg gacattgtgt gtcagccgca gtcacaagag tcctgccagc
     5641 agtggcttga agcccactgg cgtactctga caccggcgaa tttcacccag ttgcaggagg
     5701 cacaatgagc cagcatttac ctttggtcgc cgcacagccc ggcatctgga tggcagaaaa
     5761 actgtcggat ttaccctccg cctggagcgt ggcgcattac gttgagttaa ccggagaggt
     5821 tgatgcgcca ttactggccc gcgcggtggt tgccggatta gcgcaagcag atactttgcg
     5881 gatgcgtttt acggaagata acggcgaagt ctggcaatgg gtcgatgatg cgctgatatt
     5941 cgaactgcca gaaattatcg acctgcgaac caatattgat ccgcacggta ctgcgcaggc
     6001 attaatgctg gcggatttgc aacaagattt gcgcgtcgat agcggtaaac cactggtctt
     6061 tcatcaactg atacaggtgg cagataaccg ctggtactgg tatcagcgtt atcaccattt
     6121 actggtcgat ggcttcagtt ttccggccat tacccgccag atcgccaaca tttactgcgc
     6181 attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg cctttcgctg atgtagtgga
     6241 agagtaccag caataccgcg aaagcgaagc ctggcagcgt gatgcggcat tctgggtgga
     6301 acagcgtcgc caactgccac cgcccgcgtc actttctccg gcacctttag cggggcgcag
     6361 cgcctcggca gatattttgc gtctgaaaat ggaatttacc gacggggaat tccgccagct
     6421 ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc cttgcgctga cggctttgtg
     6481 gctggggcga ttgtgcaatc gcatggacta cgccgccgga tttatcttta tgcgtcgact
     6541 gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac gttttgccgc tgggtattca
     6601 tattgcggcg caagaaacgc tgccggaact ggcaacccga ctggcaaccc gactggcagc
     6661 acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc gaacaaattg tccgtgacag
     6721 cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta ctcaatatta aggtatttga
     6781 ttaccaactg gatattcctg gtgttcaggc gcaaacccat accctggcaa ccggtccggt
     6841 taatgacctt gaactggccc tgttcccgga tgaacacggt gatttgagta ttgagatcct
     6901 cgccaataaa cagcgttacg atgagccaac gttaatccag catgctgaac gcctgaaaat
     6961 gctgattgcc cagttcgccg cggatccggc gctgttgtgc ggcgatgtcg atattatgct
     7021 gccaggtgag tatgcgcagc tggcgcagat caacgccact caggttgaga ttccagaaac
     7081 cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca ccggatgctc cggcgctggc
     7141 agatgcgcgt tacctgttca gctatcggga aatgcacgag caggtggtgg cgctggcgaa
     7201 tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg gcggtggcac taccgcgctc
     7261 ggtctttttg accctggcac tccatgcaat agttgaagct ggcgcggcct ggctaccgct
     7321 ggataccggc tatccggacg atcgcctgaa aatgatgctg gaagatgcgc gtccgtcgct
     7381 gttaattacc accgacgatc aactgccgcg ctttagcgat gttcccaatt taaccaacct
     7441 ttgctataac gccccgctta ccccccaggg cagtgcgccg ctgcaacttt cacaaccgca
     7501 ccacacggct tatatcatct ttacctctgg ctccaccggc aggccgaaag gggtaatggt
     7561 cgggcagacg gctatcgtca accgcctgtt gtggattcaa aatcattatc cgctcacagg
     7621 cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat gtctcggtgt gggagttttt
     7681 ctggccgttt attgccgggg ctaaactggt gatggctgaa ccggaagcgc accgcgaccc
     7741 gctcgctatg cagcaattct ttgccgaata tggcgtaacg accacgcact ttgtgccgtc
     7801 gatgctggca gcatttgttg cctcgctgac tccgcaaacc gctcgccaga gttgcgtgac
     7861 gttgaaacag gttttctgta gtggtgaggc cttaccggct gatttatgcc gcgaatggca
     7921 acagttaacc ggcgcgccgt tgcataacct gtatggcccg acggaagcgg cggtagatgt
     7981 cagctggtat ccggcttttg gcgaggaact ggcacaggtg cgcggcagca gtgtgccgat
     8041 tggttatccg gtatggaata cgggtctgcg tattcttgat gcgatgatgc atccggtgcc
     8101 gccgggtgtg gcgggtgatc tctatctcac cggtattcaa ctggcgcagg ggtatcttgg
     8161 acgacccgat ctgaccgcca gccgctttat tgccgatcct tttgctcctg gtgaacggat
     8221 gtatcgtacc ggagacgttg cccgctggct ggataacggc gcggtggagt acctcgggcg
     8281 cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa ctgggcgaaa tcgatcgcgt
     8341 gatgcaggcg ctgccggatg tcaaacaagc cgttacccac gcctgtgtga ttaaccaggc
     8401 ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat ctggtatcgc aatcgggcct
     8461 gccgttggat accagcgcat tgcaggcgca gcttcgcgaa acattgccgc cgcatatggt
     8521 gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc gccaacggca agctggatcg
     8581 caaagcctta ccgttgcctg aactgaaggc acaagcgcca gggcgtgcgc cgaaagcggg
     8641 cagtgaaacg attatcgccg cggcattcgc gtcgttgctg ggttgtgacg tgcaggatgc
     8701 cgatgctgat ttctttgcgc tgggcggtca ttcgctactg gcaatgaaac tggcagcgca
     8761 gttaagtcga cagtttgctc gtcaggtgac gccggggcag gtgatggtcg cgtcaaccgt
     8821 cgccaaactg gcaacgatta ttgatggtga agaggacagc tcccggcgca tgggattcga
     8881 aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg ttttgtttcc atccggcgtc
     8941 cggttttgcc tggcagttta gcgtgctctc gcgttatctc gatccacaat ggtcgattat
     9001 tggcattcag tcgccgcgcc ctcatggacc catgcagaca gcgacaaacc tggatgaagt
     9061 ctgcgaagcg catctggcaa cgctacttga gcaacaaccg cacggcattg cggcgcggct
     9121 gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg gatacctggc cgccagagac
     9181 gcaaaactgg caggaaaaag aggctaatgg tctggacccg gaagtgctgg cggagattaa
     9241 ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt acttcaacgg agttgtttac
     9301 caccattgaa ggcaactacg ctgatgctgt gcgcctgctg acgactgctc atagcgtacc
     9361 gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg cttcaggaag gtatgagccc
     9421 cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc tatcgtcagg attgtgcgca
     9481 tgtggatatt atctctccag gggcgtttgt aaaaattggg ccgattattc gcgcaacgct
     9541 aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAZXUL010000145         9558 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZXUL010000145 DAZXUL010000000
VERSION     DAZXUL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9558
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.145"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACH3L1_003642"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACH3L1_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFT4078230.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="ACH3L1_003643"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="ACH3L1_003643"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFT4078231.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="ACH3L1_003644"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="ACH3L1_003644"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFT4078232.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="ACH3L1_003645"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="ACH3L1_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFT4078233.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="ACH3L1_003646"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="ACH3L1_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFT4078234.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="ACH3L1_003647"
     CDS             4025..4615
                     /locus_tag="ACH3L1_003647"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFT4078235.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="ACH3L1_003648"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="ACH3L1_003648"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFT4078236.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="ACH3L1_003649"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="ACH3L1_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFT4078237.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="ACH3L1_003650"
     CDS             complement(6551..6658)
                     /locus_tag="ACH3L1_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFT4078238.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="ACH3L1_003651"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="ACH3L1_003651"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFT4078239.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="ACH3L1_003652"
     CDS             7648..7875
                     /locus_tag="ACH3L1_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFT4078240.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACH3L1_003653"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACH3L1_003653"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFT4078241.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..>9558)
                     /locus_tag="ACH3L1_003654"
     CDS             complement(9447..>9558)
                     /locus_tag="ACH3L1_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078242.1"
                     /translation="GNHYDVYLPPELFSQAYKLKNKEMNAQLDYLNRYAT"
BASE COUNT         2156 a         2501 c         2540 g         2361 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgcca
//
LOCUS       DAZXUL010000146         9538 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZXUL010000146 DAZXUL010000000
VERSION     DAZXUL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.146"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACH3L1_003655"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACH3L1_003655"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFT4078243.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACH3L1_003656"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACH3L1_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFT4078244.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACH3L1_003657"
                     /pseudo
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACH3L1_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4131..4886
                     /gene="actS"
                     /locus_tag="ACH3L1_003658"
     CDS             4131..4886
                     /gene="actS"
                     /locus_tag="ACH3L1_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFT4078245.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="ACH3L1_003659"
     tRNA            4965..5038
                     /locus_tag="ACH3L1_003659"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6783
                     /locus_tag="ACH3L1_003660"
     CDS             5173..6783
                     /locus_tag="ACH3L1_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4078246.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6815..7234
                     /locus_tag="ACH3L1_003661"
     CDS             6815..7234
                     /locus_tag="ACH3L1_003661"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078247.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKVSWR"
     gene            8353..9099
                     /locus_tag="ACH3L1_003662"
     CDS             8353..9099
                     /locus_tag="ACH3L1_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFT4078248.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9397..>9538
                     /locus_tag="ACH3L1_003663"
     CDS             9397..>9538
                     /locus_tag="ACH3L1_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078249.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLP"
BASE COUNT         2432 a         2362 c         2421 g         2323 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtctagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acacttctta aagtctacta acttactgaa tcctaaggga tacggcttaa cgccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgaagtagtg agcagaccta
     5161 aggtctcagt acatgctcaa gtcaagaacc tacctttatc agcgtaatgg tgtcttttac
     5221 atccgtctta ggatgaagac cactagtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc tttacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc catgcagaca accctaacgc ctcttatgag
     5401 gagctacggg agcacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgtgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgacat taagtccaac
     5521 cttcgggaag ctgtagcgac cgagcgtctg actgtagacc agcatcgcta tattaatgac
     5581 gttatcggtg tgcttaaggc ctgtcaggat agactcaatg gcgatagctc tggcctattg
     5641 tcttaccttg agcctgagac tggcagtcta agaccgtccg tctccctatc tgtattggcg
     5701 gaacctgaag tccctgaacc taaagcactg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg cccagaactg gaagccagcg accctaagcg aaaaccgagc gtcacactct
     5821 acgctaatcg agatattcga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gagttcgtga agtcctccag cagctaccga agaaccgcaa gcaacgcttt
     5941 aagtctatgc cgctgtctga cttgctgaac cgggagtcta agactgactg tctggacgtc
     6001 gttaccatta acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacttga tagctaagaa cctgaccgaa ggtcttgagc tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtgatgc gttcagtcct gagcaggtag ggcaactact ggtcgcagct
     6181 aaagcgtact cgcagaagac ctcaggtaag ccataccatt attacgtcac cgctctggct
     6241 gcgattacag gtgcgagact taacgaggtg gcccaacttc aggttaaaga tgtcaggacc
     6301 actgaggctg ggaccgtttt tatccatatc aacgaagacg atagcagcct gccgggtaag
     6361 agtatcaaga acgcccacag tgaccgctgt gtcccgttgg tagatggtgc ttatggtttt
     6421 gtattggctg actttatgtc actcgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtgttca atggcctcaa gctaatgaag aacggctacg gtgagcaggt tagcaagtgg
     6541 tttaaccgaa cgttgctgcc caaagtcctc gctgaccgta gcggcttagc ttttcactcg
     6601 tttagacata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtggaaacgc tgaaagaaaa attagcggag tcattgtcag taaaaaaaat agacggaaag
     6781 taatcgcata gggatagcgg aatgtgagga cgtaatggtc aaagaatttt tagttagcaa
     6841 agaaaataag ccttcatatg aatgtatgaa tactgctgaa acttgtttgg ggctggctgg
     6901 aatggcttcg gaagattggc aacgtgagca tcattgccgc acaggtataa tatttactgc
     6961 ttttgctatt gaggcaatgt tcatcttcta tagaaaacag gttgaccccg gctacgataa
     7021 gacgcagaaa gagtgcagaa agacaatgca caagaatacg ttaaaattgt gcggtataaa
     7081 caattatatg gggacaaaac cttaccaaat tatcaaagag tgtcttgagg taagagacgc
     7141 tattgctcac ggcgattcgt atacttcgag ctttaatttc tcggcggatc atttggataa
     7201 ccaagacgat attgcaaaag tgtcctggcg gtaaactctg aacaatttcg gggattaact
     7261 gttgagcgcc ttgcaaaata cattgaaatg gcaaagcata ttgaccatga gatttctgat
     7321 aatggttaca ggccttctga aggttacctt ccagcagccg agcgcagaag tctgatgtta
     7381 gcttttggcg tcagtggtgt gtcaatgtgg cccagtgctt gagcgataat acacctcact
     7441 atagaggaat ggacccttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat
     7501 agcagtcacg acccatacac acattcatta agtctcaggc ttccggtagc ggaccgattg
     7561 gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccatt ccttttaacg
     7621 taagctatct tcccttttga caggcgggga gcagtagacc acatccgact ttaagttggc
     7681 tctgtgataa tcgctgcgcg gataactgaa gaaccaagac accattactg agttctgcca
     7741 tccatttgtg gacaggcgga aactcaaaga ggttagatca gtggtgtctg aggttggagc
     7801 tttgagtctt gaccagcagg ctggaggaga ccatgagttt atcttgttat ggattgtgtt
     7861 atcacaattc caataagaag aactttaagt ccagcctcca ctcccgccaa cccgaagtaa
     7921 aattatggtc gctatgatgt ggtctccatg cttcgggtct tactggaagt actgaacact
     7981 ttcgcgtcaa gttctccgaa gttcattcct gtttgactaa actatcacta tagggaccat
     8041 atctcgcctc tgacctcctg agagacggca agcgagaaac cgtggtccac gctagcaagc
     8101 tagccaccag cctaaggcaa gacagcctct ggtcagcctg tgtgttcttt ggtctcgtga
     8161 cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa ccttcatctc tatggtcatc
     8221 gcagaccact aaccccaatt acctcaagtc attctgtttg cgtcctgttg cctgctggtc
     8281 tccctttacg tgggggcttg agtaggtcag tggggtgcga cttcagggtg caaacaaaag
     8341 gagtacacgt aaatgtccca acaagaaatc agcatcatta acctcgacca gcttgtgtcc
     8401 atgacctcag ttgaaattgc tgagctgaca ggaaaagaac ataaacacgt tttacgagat
     8461 attcgtaata tggtcgaaga gcttaacggg gccaaaactg agcactgctc aactttgagc
     8521 agcgagctta acgggtccaa atttgggctc gttggcgagg aagtatacaa ggacgcgaaa
     8581 ggtgagagcc gaacaatgta tcgtctcgac cgtaagcata ctttcattct ggtcgctggc
     8641 tattccgttc acctccgcgc caagtgttac gaccatattc agacgttaga gcgtcgagtc
     8701 cttcaactgg aagaccagaa gaagcgagca gccattcagt ccgctaaccg ccgaggcgtg
     8761 acttggggcg actactgcaa aacctatggt ctgcccgctc agaagctaat gaccgcactt
     8821 ctccagcatc gtggcctctt tcgtaagaac ccaatcagta acgagtggtc tgtaaatccg
     8881 aagtattccg actgctttcg tatcattaag ccgtcagacc agaagttctc agcaggtggg
     8941 tataacttcc ggttcaacgc taaggggctg gaggtcttcg gtaagcctga gatggttgac
     9001 aagatgcgag gcatccttat tgcattcact ggcaccgacc agcagaagca agagcacctc
     9061 ctgaagctgg cacaatccgg taaggtggag ggaatctaag atgatgattg acccgcgcac
     9121 acctgagggg cgcatgactc tccgttaccg tggctaccgg actgaagtcc tcctcagaga
     9181 gcttggtcta gacccggaag atgagacccg ccagcaccaa tcccgagacg agctaatagc
     9241 ccaactggtc gctatgaaac tgccgcttaa ccgttagtag ctcacctact caatacctaa
     9301 tttaaacacc ttttgagatg accgatgcgg ggagacccgt aggccttact gtgcctgcga
     9361 taccgatagt ctcaactctc aagaggagaa ttacctatga tgttcaacaa caacaactgg
     9421 aagctgtcag tcaccgacat caacctttat gaaaacactg tgagtctgga cggtcagtcc
     9481 tatccgttgt cgctcgctat caagaccctc attccgggct acctgtcagg cttaccgt
//
LOCUS       DAZXUL010000147         9523 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZXUL010000147 DAZXUL010000000
VERSION     DAZXUL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.147"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..593)
                     /locus_tag="ACH3L1_003664"
     CDS             complement(<1..593)
                     /locus_tag="ACH3L1_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFT4078250.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACH3L1_003665"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACH3L1_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFT4078251.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="ACH3L1_003666"
     CDS             complement(1422..1961)
                     /locus_tag="ACH3L1_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4078252.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACH3L1_003667"
     CDS             complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACH3L1_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFT4078253.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNIGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3055..3651)
                     /gene="fimE"
                     /locus_tag="ACH3L1_003668"
     CDS             complement(3055..3651)
                     /gene="fimE"
                     /locus_tag="ACH3L1_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFT4078254.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4129..4731)
                     /gene="fimB"
                     /locus_tag="ACH3L1_003669"
                     /pseudo
     CDS             complement(4129..4731)
                     /gene="fimB"
                     /locus_tag="ACH3L1_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6185..6901
                     /gene="nanC"
                     /locus_tag="ACH3L1_003670"
     CDS             6185..6901
                     /gene="nanC"
                     /locus_tag="ACH3L1_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFT4078255.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6921..8027
                     /gene="nanM"
                     /locus_tag="ACH3L1_003671"
     CDS             6921..8027
                     /gene="nanM"
                     /locus_tag="ACH3L1_003671"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFT4078256.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8092..9072
                     /locus_tag="ACH3L1_003672"
     CDS             8092..9072
                     /locus_tag="ACH3L1_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFT4078257.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            9182..9387
                     /locus_tag="ACH3L1_003673"
                     /pseudo
     CDS             9182..9387
                     /locus_tag="ACH3L1_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         2788 a         2078 c         1997 g         2660 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgat attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
     2701 cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt tgttggctta
     2761 atattctatt attatcttta tttatagatt tttatattgc atgaggtggt ttttggagag
     2821 aagaatgagg aagatgcgtc gagccacaga aacgttagct ttacatatag cggaggtgat
     2881 gtgaaattaa tttacaagag aaataattta catatcaaat agttagatgc tttttgtcgt
     2941 tttttaatat ttttatgctt gagaaaaaat acgtaactta tttatgatat ggacagtttg
     3001 gccccaattg tcttgtgttt atttgttttt taactttatt atcaataagt taaatcaaac
     3061 ctcttctctt tttaattttt cgtttatgag attatttctt tcccataatc cggcaaaacg
     3121 agcagcatta ctggcggtat aacgcacagt atggcgaata tttcgatgcc cgagataatc
     3181 ctgaattaaa cgggtatctg caccacgctc cgccagttca taaccgcaag catgccttaa
     3241 catatgagga tgagtctgcg ttacggttcc agcttcaata ccggcatcgc gaataatgcg
     3301 ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatag cgtcggtccg
     3361 gtcagcgcct ttccagttag cacgttcctg ggtccagcgt tccacggctt cacgctcatc
     3421 aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat ttattcgacc
     3481 ttcattaagg tcaaggtcct gataatgcag atcaagcagt tcactaatac gcatcccatg
     3541 ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt aacaaaccgc
     3601 ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca caatagtagt
     3661 actcctaact gagatatatt tgaatgaata cctataggaa acctcaatcg gtcaaaatta
     3721 gcccaataga taaaaagata accacacacg aagttatgtg gctatcagtc aattacatta
     3781 aacatcatac aaaaataaaa tatcactgat gtgacaacat ctattttcat tgatttgatt
     3841 caatataaca atagattaca gaggattaac ttccagttac ttaaaaaata aagaaaaaag
     3901 tgtggaaaca ggagaattta agtgatgtgt aaacttatga atgattttgt ttatttattt
     3961 tgaagaacat aaatcattag attttgtatg ggtatggtta acttaatgtt tattatgata
     4021 attaagaatc acacttacaa taacatcaat caataaatgg ttaatcattt ttgatatatc
     4081 gtaagaataa tgtagttttt aacaccatcc ctggtatctc aactatctct ataaaacagc
     4141 atgacgctgt cgtcctctgg ctctatccca gatgccgtaa aaacgccctg cattgctggc
     4201 ggtataccag acagtatgac gaatattgcg atgcccaaga taatcctaga taagtcgcgt
     4261 atctattccc atattcgcca aagcaaaacc acacgaatag cgtaacatgt gcggatgaat
     4321 ctccagtgac aacccggcat taccaccaga agtcgagata atatggtaaa actgttgccg
     4381 agaaagcgga ttccccttac gtgataaaaa tacccactcg ctctcagcat gcggataaga
     4441 agtacggata ctcaaccagt tttttaaagc ctgaacttct ttgttcaata gcgggtgcgt
     4501 tgttgaaaag ccttttttta atcgatggat atatatacac tttgctttaa gatcaatatc
     4561 cgaaatcctc aatcgacaaa tttcactcgc ccggaaacca tgaataaaac aaagcaaaat
     4621 cagacaataa ttacgtgttg catgaggccc ggtatttgct gctttaagga gtgactcgat
     4681 ttcactatgg gtaaggaagt tccttttttt gttatcagcc ttattcctca tcgttttccc
     4741 ttataattac agacgcgcac tagctgtgct gggttagcaa taatccaaca tttttatcct
     4801 gattatgttt ataaaagcga acgtttgctt aataacttac gtaagtggac cagttcttct
     4861 gagcgaactt aaatggagta gcgatagtta cttataataa ctttgccata caaacgccat
     4921 gctcttgcat gctatgtacc tttatatatt tatcaatctg atacgaatct aataataacc
     4981 ctcatcatta atgcaactaa tcttatctat atatcatgtg atatgttctg taacaagtaa
     5041 aatcaacata aaaaattcag caaaatttaa tatttgttaa actagccgtc tcaaatatga
     5101 cattattagt taactgcatt tgccacaaaa tggctatttc atggaacgca gtaatttctt
     5161 agtggctatt atcatgctaa ttcttagatc cctagcgatt attcctgcct ggttcgtcag
     5221 cttaggccat gagattgtta cattcctttt tatcctggag atatggggga acgcaggcga
     5281 cgctttttca tctacatccg ttggtaaata tagctttggt cactacgact taagccttcc
     5341 tcaatcacag ctattcgccg ggcgatacgg caaacaaact aagtgaaaca tcatcctgaa
     5401 aacaccaaca tcaacaagcc tctccagatc gacatcagga gtgaccagtt acaagccaca
     5461 aaccaagaaa catatttcat gatgagaatt atgctcagcc tgatggcgag taaaccctta
     5521 ttttcaccac tctcactgag ttagcttgca aagctttctt tgtttagatt ctaaataaat
     5581 tatttataaa acgtatagtt aaaagaaaat ctttagtttt tgaagaccac cgcaagtgtt
     5641 cgtctggctt cacatggcat cttcttctta gaaaaagatc gacatatttt gtgacgcgaa
     5701 ttgcaaatct ggttttgttg tatggattgc gtgatttttg atctggtata acaggtataa
     5761 agatgcacca cgatagtcaa tgagactggg catctcgtaa tctatggcaa acatcacttc
     5821 agttctttca catcgggtga tgaaaatgca cttcagtctg aaaggaatat gaaaatgaga
     5881 tcaacagaca ctctatttta tgactctggg taaaatgggt tgagtaagtg atataactta
     5941 cgaatattca aatcaattaa aaatcagaag agattttata ctcaggtatt taatctggat
     6001 ctctgtttat ttaaataatg ttaaaagaga attttcacag gagaccttat acaaaaaata
     6061 taaaatacag ttacccgttg ccaaagacac tataagcctg gcaaaaaaat attacacaac
     6121 ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca aatgagtgaa
     6181 atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt cgtccccatt
     6241 aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa gccacgccta
     6301 tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag
     6361 taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca atgatgttca
     6421 ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt
     6481 aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt cctgggacgc
     6541 gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt accgacaaca
     6601 agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat atgtcactta
     6661 ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca aacagaatga
     6721 ttatcgctat gcaaaccaca agaaatgggc gacggaaaat gcctttgttc tacaatacca
     6781 tatgacgccc gatattacgc catacataga atatgactac cttgaccgcc agggtgttta
     6841 caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta
     6901 gtagacagga gacagtcaca atgaataaaa ctataatggc gcttgctatc atgatggctt
     6961 catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa agtggtaccg
     7021 gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtaca gcatggtaca
     7081 agctagatac acaggccaaa gataaaaaat ggactgcgtt agctgcattc cctggtggac
     7141 cgagagagca agcaacctct gcatttattg atggcaatct gtatgtgttt ggcggcattg
     7201 gcaaaaacag cgagggattg actcaggtat ttaatgacgt acacaaatac aaccccaaaa
     7261 ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggt catgtgactt
     7321 ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc ttcaatggct
     7381 attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa atcaacgccc
     7441 actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg tcttttgatc
     7501 cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg gctggtgcgg
     7561 cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa ccaggattgc
     7621 gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg aataagcttg
     7681 atcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc aatgattctc
     7741 ttatatttgc tggaggggcc ggattcaaag gttcacgaga aaattaccag aacggtaaga
     7801 actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt tggcataacg
     7861 ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta tcattgccct
     7921 ggaataatag tttattaatt attggcggtg aaactgcagg cggcaaagcg gtgacggatt
     7981 cagttttaat ttctgtgaag gataataaag tcacagtaca aaactaacgc ttcagggccc
     8041 cggtaaaggg gccctgctat caatttgtca tttgtattac gcgaaaatta tatgaacgca
     8101 ataatatcac ccgattatta ctatgttctt accgttgctg gtcagtctaa tgccatggcg
     8161 tatggcgaag gactgccatt accggacagg gaagatgcgc ctcattccag aattaaacaa
     8221 ttagcgagat ttgcgcatac gcatcccgga ggcccgccat gtcactttaa cgacattatt
     8281 ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca tcctctggca
     8341 acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc acggaaatta
     8401 ctgcccttca ttcctgataa tgcagggatt cttatcgttc cgtgttgccg tggcggatcg
     8461 gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag ccatgatgct
     8521 tgtcgttggg gaacggatac tccgctgtac caggatttag tcagcagaac gcgagccgca
     8581 ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg cgaatttgac
     8641 ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt tgaagccttt
     8701 cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc gccgtggttt
     8761 tgcggcgata ccacctggta ctggaaagaa aatttcccgc attcgtatga agctatttat
     8821 ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca gcaacaaggt
     8881 gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac gggatattac
     8941 ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag tcatttcagc
     9001 acggcagccc gtcgggggat tatttctgac agatttgtag aagcaatttt gcagttttgg
     9061 cgcgaaaggt gagcggggag tttattcatg ggggatgtcc ttgttcgttc tcagagtaga
     9121 caacaaaaaa caaatgagat caaggtaata tctcttcact tgcgggctcg aacagcaggc
     9181 aattgccaat ataataatct ggcacaaggg tgaaaaacgc gcaccgcata aaccgttgtt
     9241 attgcttaca gattagccgg atgccttaat agacatccgc gccttttcga ttatggctcg
     9301 gaaatctacg aacatctaca cagtttactg gaacgttttg ggccacagcg ttcgcagcac
     9361 cgtcctgata tgccgttctg gcgattataa gtcgacggat tctggcaaat agacgatgat
     9421 gatatgctta atgatgttca aagaaattag gccattatta tattattgct tgcattttct
     9481 ttcattccta aaagcacccg ccattaatcc caggcgcatt atg
//
LOCUS       DAZXUL010000148         9494 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZXUL010000148 DAZXUL010000000
VERSION     DAZXUL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.148"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..190)
                     /locus_tag="ACH3L1_003674"
     CDS             complement(<1..190)
                     /locus_tag="ACH3L1_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFT4078258.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="ACH3L1_003675"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="ACH3L1_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFT4078259.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="ACH3L1_003676"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="ACH3L1_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFT4078260.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="ACH3L1_003677"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="ACH3L1_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFT4078261.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="ACH3L1_003678"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="ACH3L1_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="ACH3L1_003679"
     CDS             complement(3262..3381)
                     /locus_tag="ACH3L1_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFT4078262.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACH3L1_003680"
     CDS             complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACH3L1_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFT4078263.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /gene="ymgI"
                     /locus_tag="ACH3L1_003681"
     CDS             3628..3801
                     /gene="ymgI"
                     /locus_tag="ACH3L1_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFT4078264.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="ACH3L1_003682"
     CDS             3803..4147
                     /locus_tag="ACH3L1_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFT4078265.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="ACH3L1_003683"
     CDS             4151..4486
                     /locus_tag="ACH3L1_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFT4078266.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="ACH3L1_003684"
     CDS             complement(4543..6864)
                     /locus_tag="ACH3L1_003684"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFT4078267.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="ACH3L1_003685"
     CDS             complement(6981..7190)
                     /locus_tag="ACH3L1_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078268.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7590..7809)
                     /gene="ymgF"
                     /locus_tag="ACH3L1_003686"
                     /pseudo
     CDS             complement(7590..7809)
                     /gene="ymgF"
                     /locus_tag="ACH3L1_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
     gene            complement(7941..9464)
                     /locus_tag="ACH3L1_003687"
     CDS             complement(7941..9464)
                     /locus_tag="ACH3L1_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFT4078269.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2660 a         2206 c         2079 g         2549 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc acaaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgctgg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcagccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa taca
//
LOCUS       DAZXUL010000149         9440 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZXUL010000149 DAZXUL010000000
VERSION     DAZXUL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.149"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1154
                     /locus_tag="ACH3L1_003688"
     CDS             <1..1154
                     /locus_tag="ACH3L1_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078270.1"
                     /translation="EDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKY
                     SAKDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNV
                     PKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACE
                     AHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFN
                     QSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVM
                     GCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKD
                     LFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="ACH3L1_003689"
     CDS             1833..2051
                     /locus_tag="ACH3L1_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFT4078271.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /gene="yiiF"
                     /locus_tag="ACH3L1_003690"
     CDS             2269..2511
                     /gene="yiiF"
                     /locus_tag="ACH3L1_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFT4078272.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACH3L1_003691"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACH3L1_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFT4078273.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACH3L1_003692"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACH3L1_003692"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFT4078274.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="ACH3L1_003693"
     CDS             complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="ACH3L1_003693"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFT4078275.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="ACH3L1_003694"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="ACH3L1_003694"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFT4078276.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..9391
                     /gene="fdhD"
                     /locus_tag="ACH3L1_003695"
     CDS             8558..9391
                     /gene="fdhD"
                     /locus_tag="ACH3L1_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFT4078277.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2311 a         2315 c         2446 g         2368 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     9301 agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     9361 ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa
     9421 ttgcaggact tattcatttc
//
LOCUS       DAZXUL010000150         9302 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZXUL010000150 DAZXUL010000000
VERSION     DAZXUL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9302
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.150"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="ACH3L1_003696"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="ACH3L1_003696"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFT4078278.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="ACH3L1_003697"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="ACH3L1_003697"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFT4078279.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="ACH3L1_003698"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="ACH3L1_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFT4078280.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="ACH3L1_003699"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="ACH3L1_003699"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFT4078281.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="ACH3L1_003700"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="ACH3L1_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFT4078282.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="ACH3L1_003701"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="ACH3L1_003701"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFT4078283.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="ACH3L1_003702"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="ACH3L1_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFT4078284.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="ACH3L1_003703"
     CDS             complement(8064..9206)
                     /locus_tag="ACH3L1_003703"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFT4078285.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..>9302)
                     /locus_tag="ACH3L1_003704"
     CDS             complement(9215..>9302)
                     /locus_tag="ACH3L1_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078286.1"
                     /translation="KEKYKHLLYQKKFLDGVFTAIKYNVMKG"
BASE COUNT         2576 a         2161 c         2044 g         2521 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccagaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tg
//
LOCUS       DAZXUL010000151         9165 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZXUL010000151 DAZXUL010000000
VERSION     DAZXUL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.151"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..923
                     /locus_tag="ACH3L1_003705"
     CDS             <1..923
                     /locus_tag="ACH3L1_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4078287.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="ACH3L1_003706"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="ACH3L1_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFT4078288.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACH3L1_003707"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACH3L1_003707"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFT4078289.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="ACH3L1_003708"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="ACH3L1_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFT4078290.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="ACH3L1_003709"
     CDS             4446..4805
                     /locus_tag="ACH3L1_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFT4078291.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACH3L1_003710"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACH3L1_003710"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFT4078292.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="ACH3L1_003711"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="ACH3L1_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFT4078293.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="ACH3L1_003712"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="ACH3L1_003712"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFT4078294.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2093 a         2218 c         2475 g         2379 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaagcgtatt atgtacaccc cgcgc
//
LOCUS       DAZXUL010000152         9029 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZXUL010000152 DAZXUL010000000
VERSION     DAZXUL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.152"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACH3L1_003713"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACH3L1_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFT4078295.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACH3L1_003714"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACH3L1_003714"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFT4078296.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACH3L1_003715"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACH3L1_003715"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFT4078297.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACH3L1_003716"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACH3L1_003716"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFT4078298.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="ACH3L1_003717"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="ACH3L1_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFT4078299.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /gene="smg"
                     /locus_tag="ACH3L1_003718"
     CDS             5562..6035
                     /gene="smg"
                     /locus_tag="ACH3L1_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFT4078300.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="ACH3L1_003719"
     CDS             6064..6606
                     /locus_tag="ACH3L1_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFT4078301.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="ACH3L1_003720"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="ACH3L1_003720"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFT4078302.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="ACH3L1_003721"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="ACH3L1_003721"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFT4078303.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="ACH3L1_003722"
     CDS             8003..8260
                     /locus_tag="ACH3L1_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFT4078304.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="ACH3L1_003723"
     CDS             complement(8236..8790)
                     /locus_tag="ACH3L1_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFT4078305.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2259 a         2192 c         2252 g         2326 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DAZXUL010000153         8837 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZXUL010000153 DAZXUL010000000
VERSION     DAZXUL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.153"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            5..2177
                     /gene="espL4"
                     /locus_tag="ACH3L1_003724"
                     /pseudo
     CDS             5..2177
                     /gene="espL4"
                     /locus_tag="ACH3L1_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2146..3883)
                     /gene="aceK"
                     /locus_tag="ACH3L1_003725"
                     /pseudo
     CDS             complement(2146..3883)
                     /gene="aceK"
                     /locus_tag="ACH3L1_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4067..5371)
                     /gene="aceA"
                     /locus_tag="ACH3L1_003726"
     CDS             complement(4067..5371)
                     /gene="aceA"
                     /locus_tag="ACH3L1_003726"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFT4078306.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5401..7002)
                     /gene="aceB"
                     /locus_tag="ACH3L1_003727"
                     /pseudo
     CDS             complement(5401..7002)
                     /gene="aceB"
                     /locus_tag="ACH3L1_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7271..8200)
                     /gene="metA"
                     /locus_tag="ACH3L1_003728"
     CDS             complement(7271..8200)
                     /gene="metA"
                     /locus_tag="ACH3L1_003728"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFT4078307.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8357..8800
                     /locus_tag="ACH3L1_003729"
     CDS             8357..8800
                     /locus_tag="ACH3L1_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFT4078308.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2303 a         2143 c         2148 g         2243 t
ORIGIN      
        1 tcgtattcct cgtagttctt tctctgcaaa tattaataat acagcccaga caaatgaaca
       61 ccaaaccctg agtgaattgt tttataaaga actcgaggat aaattttctg gcaaggagct
      121 ggcgacgcct ctattaaaaa gcttctcaga gaactgtaga caaaatgttc gtcatatctt
      181 tagcaacaag gattttgtca ttaaattttc cacgtccgtc ttacaagctg ataagaaaga
      241 aattacgatg attaataaaa acgaaaacac gacactcact caaaccattg ctacaatatt
      301 tgaagaatac ctaatggaaa ttttacctca acgctcagac actcttgata aacaagaatt
      361 aaaccaaaaa tcagatagaa aagaaaaaga attcccaaga attaaactta atggtcaatg
      421 ttatttcccg gggcgcccca aaaccgtata gtataccgac acattgctgc acaatatatt
      481 aatgatattt atcagaatgt tgattacaaa ccccatcaag atgattactc ttcagctgaa
      541 aaatttctca ctcacttcaa caaaaaatgc aaaaaccaga ctttggcgtt gatttccagc
      601 cgtcctgagg ggcgttgcgt tgctgcctgc ggtgatttcg ggctagttat gaaagcatat
      661 tttgacaaga tggaatcaaa tggcatcagt gttatggcag ccatattact ggtggataac
      721 catgctttga cggtccggct aagaataaag aacacaactg aaggatgtac ccattacgtg
      781 gtttcggttt atgatcctaa tgtaactaac gataaaataa gaattatgag cgaaagcaaa
      841 gaggatatta aacactattc tctgatggat tttatgaatg tagattatag cctcctgaaa
      901 tggtcaaatg atcatgttat taaccaatct gttgcaataa ttccagcact tccgaaagaa
      961 cagctattga tgttaaaagg aactgtggat gaaataaccc ctccattatc accagcaacg
     1021 atgaatttgc taatggcaat tggtcagaat caccaactta cgcaactgat gattcagctc
     1081 cagaaaatgc cagaactaca tagaacagaa atgttgactg cctataatag tattaacctc
     1141 cccggtttat atttggctat aaattatggc aatgcggata tcgttgagac tattttcaat
     1201 tcattgtcag aaacaggata tgaagggtta ctctcgaaaa aaaatctcat gcatattctg
     1261 gaggcaaaag ataaaaatgg tttttctgga ttatttttag cgatatcacg taaggataaa
     1321 aatgttgtaa cctcgattct gaacgtctta cctaaactgg ccgcaacaca tcatttagat
     1381 aatgaacagg tgtataaatt cctgagtgcc aaaaatagaa cgtccagcca tgttttatac
     1441 catgttatgg cgaatggtga tgccgacatg ctgaaaattt ttttggtcgc gttacctttg
     1501 ttaattcgca catgtcattt gactaaagaa caggtactcg atctcctgaa ggcaaaggat
     1561 ttttatggtt gcccaagact atacctggcg atgcaaaatg gacatagcga tatcgtgaaa
     1621 gttattctcg aagcattgcc ctgtctggcc caggaaatta acatttcagc ttccgatatt
     1681 gtagatcttc tgaccgctaa aagtcttgcg cgcgacacgg gtttgtttat ggccatgcag
     1741 cgcggacata tgaacgttat taatactatt tttaacgcat tacccactct ttttaatacg
     1801 tttaaattcg ataaaaaaat atgaagcccc tcctcctggc aaataattct aatgaatatc
     1861 ccggtttgtt ttcagcgata cagcataaac aacaaaacgt tgtagagacg gtttatcttg
     1921 ctttatctga ccatgcacgc ctgtttggat ttaccgctga agatattatg gatttttggc
     1981 aacacaaagc gccacaaaaa tactctgcct ttgagttggc ttttgaattt ggtcaccggg
     2041 ttattgctga attaatcctt aatacattaa ataagatggc tgaaagcttt ggctttacgg
     2101 ataaccctcg atacattgcg gagaaaaatt atatggaagc tttactcaaa aaagcatctc
     2161 cccataccgt acgctaaatc tttgcctgcg ccgatacgca taaacatctt ccacatgccc
     2221 ttcacgtatg cggttttgta gtgcgcgcca gtaatcagcg cggaacaggt cggcgtgcat
     2281 ctcttcaaac agcggaccaa tacgcgggtc ggcgcatagc cagtggcgaa actcttccgg
     2341 gaaaacatcg cccggcgaga cgctgtacca cggttcgctg gcaagttcgt cttccggata
     2401 gcgcggcggc gggatgtcgc ggaaattcac ttccgtcatg tagcaaattt catcgtaatc
     2461 ataaaaaacc acacgcccgt gacgggtgac accgaagttt ttaaacagca tgtcgccagg
     2521 gaaaatgtta gcagcggcaa gctggcgaat agcgttaccg tattcttcaa tggcgtcgcg
     2581 caactgctga ccttccactt gttccagcca gatattgagc ggcaccatcc gccgctcgat
     2641 tatagagatg gcgaattaca atctgctcac caagatcggt gattttttcc gctgcttcct
     2701 ggagcagtaa ttccattaat accggggaaa tatgccgctt ctccagcaca aagttttcaa
     2761 actcctgggt gtccgccatt cggcccacac ggtcgtgctc tttcaccagt tgatagcagg
     2821 cacgaacgtg agcggcagac atctctttct gcggcgcgaa cttgtctttg atgactttga
     2881 atacccgatc aaagcccggc agcgtaaaca ccaacatcac cattccacga atacccggcg
     2941 cttcaatgaa ctgctcatta cagccctgta gataaacgag atattcgcgg tagctttcgg
     3001 ttttggcgtg cttctggcag ccgatagcca tatacaattc agcagtggtt ttacctggca
     3061 gaatttcccg tagccactcg accagcgctg cgggcagcgg tgcataaacc ataaaataag
     3121 aacgcgcaaa gccaaaaaca atgctcgctt cggcggtcgt cgtcaggcag gtatcaataa
     3181 ataactcgcc gtcgtccgtc tggtggatcg gcagtaagaa gggcaacgtg ccggaaggcg
     3241 tgatcagttt gcccaccagc caggcggctt tattgcggta aaacagttcg ttcgccacct
     3301 gtaaatgact ttccgcgagg ttgtctgtcc ccagcgtttc cgtcagatgg cgaatgatgt
     3361 aatgaatgtc acggctttta ttctgccagc gcaggcgcag cggcaggtcg ctgataacgc
     3421 gcatcagtag agattcccag ccgtgatcgg ggtgaaagtc tttcgccagc gggcggggaa
     3481 tggtacgaaa gcggcgctct ggctgagagc taaagataaa aagccgctcg ggagtaagcg
     3541 agcggtggtc aaataaccga cagtacacgg agttaaaaaa gctctccgca atctcgaagc
     3601 gcgggtaatc cggcaacagc cgcgtgtaat gctctttaac gcgcagtaaa aatgccgcgt
     3661 ccgtactttg gccgttagta atgcagcgca gttgctccac gaccagacca acgtgatgat
     3721 cgtaaagatg gatacggttt ttcatcgcct gctggacagc atgccagtcg gcctgttcga
     3781 aacgctgctg cgccccggaa gtcacttcga ggaatcgacc atactgagcg tcgaagcctt
     3841 gcaaaatggt ttgagcaatc agtaattcca ggccacgcgg catttagcgc cctcatcagg
     3901 agcagagaat tgccggatgc ggcgtaaacg ccttatccag cctacgttcg gcaacggttg
     3961 taggcatgat aagacgcgca agcgtcgcat caggcatcgt gcaccgattg ccggatgcgg
     4021 cgtaaatgcc ttatccggcc tacagtcagc aacgtttgta ggttgcttag aactgcgatt
     4081 cttcagtgga gccagtcagc gcggtgactg aagacgtgcc gccctgaata atagtcgtca
     4141 ctttatcgaa gtaacctgta cccacttcct gctggtgaga tacgaaggta tagccatctt
     4201 tcgcggcggc aaattccggc tgctgcactt tctcaacata gtgcttcata ccttcgccct
     4261 gcgcataagc gtttgccagg tcgaacatgt taaaccacat gctgtggata cctgctaggg
     4321 tgatgaactg gaacttgtag cccatatccg acagctgctg ctggaagctg gcaatagttt
     4381 tgtcgtcgag gtttttctgc cagttgaacg acggcgagca gttataagcc agcagtttgc
     4441 ccggatattt cgcgtggata gcttgtgcaa agcgacgcgc cagttccaga tccggcgtgg
     4501 aggtttcaca ccagaccagg tcagcatatg gcgcatacgc caggccacgg ctgatcgctt
     4561 gctcaatgcc cgcatgagta cggaagaagc cttcactggt acgctcgccg gtaataaatt
     4621 cgctgtcata cgggtcgcaa tctgaggtga tcagatccgc cgcatcagca tcggtacgtg
     4681 caaccagcag ggttggaacg cccgtcacgt cagctgccag acgcgccgcg accagtttct
     4741 gaatagcttc ctgagttggc actaaaactt tgccgcccat gtgaccgcat ttcttcactg
     4801 acgccagctg atcttcgaag tgaactgccg cagcaccggc ttcaatcatc gctttcatca
     4861 gttcaaaggc attcaggaca ccgccaaaac cggcttccgc atcggcaacg atcggcagga
     4921 agtaatcgac atagcgcgga tcgcccggct caatgcccac ggaccattgg atctgatcgg
     4981 cacgacggaa ggtgttgttg atccgctcca ccacagctgg caccgagttt gccggataga
     5041 gcgactgatc cggatacatg ctggccgcca ggttagcgtc cgccgctacc tgccatcccg
     5101 acagatagac tgcttcaata cccgctttcg cctgttgcag cgcctgaccg ccagtcagtg
     5161 caccgaggtt gttgatgtag ccttttttcg actcaccgtg cagcagacgc cacattttcg
     5221 ctgcgcccag ttgcgccagc gtgcattcag gattgactga accgcgtaat ttcaccacat
     5281 cttccgcact gtatgggcga gtaatgcctt cccaacgcgg ttgagtccac tctttctgta
     5341 attcttcaat ttgttgtgta cgggttttca tgtgcagatg ctccatagtt atgtggtggt
     5401 ttacgctaac aggcggtagc ctggcagggt caggaaatca attaactcat cggaagtggt
     5461 gatctgttcc atcaagcgtg cggcatcgtc aaaacggccc tgggagaaac gttcttcgcc
     5521 cagttcgctg gcaatgactt tcatctcttc gcccagcatc tggcggaaca aggctttggt
     5581 caccggtttg ccattgctca acgttttttg atgatggatc cactgccaga tcgaggtacg
     5641 ggaaatttca gccgtcgccg catcttccat caggccataa atcggcacgc agccgttgcc
     5701 ggagatccac gcttcgatgt actgcacagc cacgcgaatg ttagcgcgca taccttcttc
     5761 ggtgcgttca ccatcacaag gtgccagcag ctgatcggca gtaatcggcg cgtcttgttc
     5821 gcgcaatact tcaagctgat ttttacggga gccgagaatg tcgttgaata ccgccattgc
     5881 cgtgtccgca aggcctgggt gagcgatcca tgtgccatcg tgaccgttat tggcttccag
     5941 cgctttatcc gcttttactt tgttgagcac ctggttatta cgctcttcat ctttgctcgg
     6001 aataaacgcg gccatgccgc ccatcgcaaa agcaccgcgt ttatggcagg ttttaatcag
     6061 caggcgtgag taagcattca ggaatggttt atccatcgtc acggcctgtc tgtctggcag
     6121 gacacgatcg ggatagtttt tcaacgtttt gatatagctg aagatgtaat cccagcgacc
     6181 gcagttcaga ccaacaatat ggtcgcgcag cgcgtgaagg atttcgtcca tctggaacac
     6241 ggcgggcagc gtttcaatca gcaacgtcgc cttgatggtg ccgcgcggca gattaaagcg
     6301 atcttctgca tagctgaaga cttcgctcca ccaggccgct tcctgctagg actgggtttt
     6361 cggcagatag aaatagggac cgctgccctt tgccaacagt gcctgatagt tgtggaagaa
     6421 atagagcgca aaatcaaaca ggctaccggg gattgcctca ccacgccagg tgacatgttt
     6481 ttccggcaag tgcagaccgc gtacccgaca aatcaaaacc gctggattgg gcttgagctg
     6541 ataaattttg cctgcttcat tggtatagct gatggtaccg ttaaccgcat cgcgcaggtt
     6601 aatttgcccg tcgatcactt tgttccagtc tggtgccagt gaatcttcga aatcggccat
     6661 aaagactttc acattggcgt tcagtgcgtt gatcaccatc ttgcgctcta ccgggccagt
     6721 tatctctacg cggcggtctt ctaagtccgc aggaatcccg cgaattttcc aatcagcatc
     6781 gcgaatggaa gccgtttccg aaataaaatc aggcaacgtt ccgttatcaa tatcttgctg
     6841 ctgctgaatg cgcgctgcca gaagtttatt gcgttgtggc gtaaaatgcg tcaccagctc
     6901 agtcagaaat tctaccgctt cggcagtaag aatttgcttc tcctgctcgc catacggcct
     6961 tgtgaaagcc agttcatcgg ttgttgttgc ctgttcagtc atcgtgcagc tcctcgtcat
     7021 ggatccgaaa acttcccctc tgtgaacgaa ggaacgttgt gcgcttttcg ttcagcacaa
     7081 ctaagactac tcatttaaaa tgcaaaatca aaaacaattt ccattttaat taaaaatact
     7141 tgataactta ttgataacaa tatgaataaa atttattcac ttgctgaggt gcgtttcgga
     7201 aataaaaaag gcacccgaag gtgcctgagg taaggtgctg aatcgcttaa cgatcgacta
     7261 tcacagaaga ttaatccagc gttggattca tgtgccgtag atcgtatggc gtgatctgat
     7321 agacgtaata gttgagccag ttggtaaaca gcaaattacc gtgactacgc cagctcgctc
     7381 gcggtttgtt ttgcggatcg ttgtgcggga aatagttata cggtacatcc gggtctagtc
     7441 cggcttccac atcgcgtaaa tattcctgcg ccagcgtttg cgcatcatat tcgggatggc
     7501 ccgtcacaaa ggcaatgcgc ttatctttgc tggcaaacag atatgcatcc ccttcttccg
     7561 tctctgccag aatttccaga tcggtgtaat cacgaatcaa cgctgccgga aagtcagcat
     7621 agcgcgaatg cggtgccagg aatgaatcat caaagccacg cgtcagaagc gcatgaggat
     7681 ggagaatatg atgctcgtaa acgccagaga gtttgtcggt gcgagtttgc ttaggaatgc
     7741 cgtagaggat attgagcgcg gcctgtaccg cccagcagac aaacagcgtc gaggtgacgt
     7801 gatctttcga ccactccagc acctgtttga tctgcggcca gtaagcgaca tcattaaact
     7861 ccaccaggcc cagcggcgca ccggttacaa tcagaccgtc aaagttctgt tcctgaatat
     7921 cttcaaagtt acagtagaag ttgttcagat gctctgcggg cgtgttgcgc gattcacggg
     7981 aatcgatgcg caacagctga atatcgacct gcaaaggtga gtttgaaagc aggcgcagaa
     8041 actgattttc agtttcaatc ttcttcggca tcaggttaag gatcagaacc ttaagtggac
     8101 gaatttcctg accagacgca cgagaagttg tcatcacaaa gacgttttct tcacgcaaga
     8161 aattgacggc gggtagctcg tccggcacac gaatcggcat aacctgatta cctcactaca
     8221 tacgcttata cgtttagaca tccagatagc tgaagataac cagaaaattt gccaatgtcg
     8281 agcctgcatg ttgaaggtga gaaagtttca aggaaaaggc tacgttagaa tataagaatg
     8341 acgaaaagga gagaaaatgg ttattagtat tcgccgctca cggcatgagg aaggggagga
     8401 actcgttgcg atttggtgtc gatctgtcga tgccacccac gattttttat cagcagagta
     8461 tcgggccgag ctggaagagc tggtgcgttc ctttctgccg gaagcaccgt tgtgggtcgc
     8521 ggttaatgaa cgggagcagc cggttgggtt tatgttgtta agtgggcagc atatggatgc
     8581 gctgtttatc gatcctgatg tgtgcggctg cggcgtgggt cggatgctgg tggagcatgc
     8641 gctctcgatg gcaccggaac tgacaaccaa cgttaatgag caaaatgagc aggcggttgg
     8701 gttctataag aaggtgggtt ttaaggttac gggacgctct gaggtggacg atttggggaa
     8761 accgtatccg ttgctgaatc tggcgtatgt gggggaataa gccgcctctg ccgtgcctgc
     8821 ccaagctgga gaagacg
//
LOCUS       DAZXUL010000154         8794 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZXUL010000154 DAZXUL010000000
VERSION     DAZXUL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8794
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.154"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..263
                     /locus_tag="ACH3L1_003730"
     CDS             <1..263
                     /locus_tag="ACH3L1_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HFT4078309.1"
                     /translation="STGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANSIKSRR
                     VISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
     gene            complement(289..966)
                     /gene="istA"
                     /locus_tag="ACH3L1_003731"
                     /pseudo
     CDS             complement(289..966)
                     /gene="istA"
                     /locus_tag="ACH3L1_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
     gene            complement(1053..1414)
                     /locus_tag="ACH3L1_003732"
                     /pseudo
     CDS             complement(1053..1414)
                     /locus_tag="ACH3L1_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1753..2307)
                     /locus_tag="ACH3L1_003733"
                     /pseudo
     CDS             complement(1753..2307)
                     /locus_tag="ACH3L1_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            2450..2572
                     /locus_tag="ACH3L1_003734"
                     /pseudo
     CDS             2450..2572
                     /locus_tag="ACH3L1_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2834..3400)
                     /gene="ipgB2"
                     /locus_tag="ACH3L1_003735"
     CDS             complement(2834..3400)
                     /gene="ipgB2"
                     /locus_tag="ACH3L1_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFT4078310.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(3682..3996)
                     /locus_tag="ACH3L1_003736"
     CDS             complement(3682..3996)
                     /locus_tag="ACH3L1_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078311.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(4074..4184)
                     /locus_tag="ACH3L1_003737"
                     /pseudo
     CDS             complement(4074..4184)
                     /locus_tag="ACH3L1_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4508..5185)
                     /gene="ospD1"
                     /locus_tag="ACH3L1_003738"
     CDS             complement(4508..5185)
                     /gene="ospD1"
                     /locus_tag="ACH3L1_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFT4078312.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(5557..5694)
                     /locus_tag="ACH3L1_003739"
                     /pseudo
     CDS             complement(5557..5694)
                     /locus_tag="ACH3L1_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5776..6074)
                     /locus_tag="ACH3L1_003740"
                     /pseudo
     CDS             complement(5776..6074)
                     /locus_tag="ACH3L1_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6663..6822)
                     /locus_tag="ACH3L1_003741"
                     /pseudo
     CDS             complement(6663..6822)
                     /locus_tag="ACH3L1_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6826..8055)
                     /locus_tag="ACH3L1_003742"
                     /pseudo
     CDS             complement(6826..8055)
                     /locus_tag="ACH3L1_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8075..8422)
                     /gene="tnpB"
                     /locus_tag="ACH3L1_003743"
     CDS             complement(8075..8422)
                     /gene="tnpB"
                     /locus_tag="ACH3L1_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFT4078313.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(8422..>8794)
                     /gene="tnpA"
                     /locus_tag="ACH3L1_003744"
     CDS             complement(8422..>8794)
                     /gene="tnpA"
                     /locus_tag="ACH3L1_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFT4078314.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
BASE COUNT         2260 a         2063 c         1951 g         2520 t
ORIGIN      
        1 gttcaacagg aagaattctg gttctgagtc tgctggcaac gttgagcact atcgtaatgt
       61 ggttactcgg ttatcacgct gaaaataaag ggttacacct gaaatatcag gctaacagca
      121 ttaaatcccg gcgggttatc tcatatctga cgttagcgaa gaatgttctt cgacactcgc
      181 cacttatttt aagacgaaca gtactgagca cagttctaaa ccatctgtcc agaacctacc
      241 gaaatatggt gctggtttat tagcagtaat tttgtgggga tccctcagcc tgaaggggcg
      301 acacagtcgg ggagagaagc ccatctcctt gccgaactgc cacagcgaag gatggaaccg
      361 gtgctgaccg gtctgatatg cgtcacgttg cagaaccaca gttttcatat tgtcatacaa
      421 cacttcgcgc gacacaccac caaagaagcg gaacgcatta cgatggcagg tctccagcgt
      481 gtcataacgc atattgtcag tgaattcgat gtacaacatt cggctgtatc cgggaacagc
      541 aacgaacacg tgaagcggtg agcgaccatt acgcatagtg ccccagtcaa cctgcatctg
      601 tcgtccgggt tcagtttcga accgaacggc aggctcctgc tcctgaggaa ccgagagaga
      661 acgaatgaat gccctgagaa tggtcattcc gccacgatat ccctggtctc tgatctcgcg
      721 agcgattacc gttgccggga ttttgtaagg atgagcatcg gcgatgcgtt gacgaatata
      781 atcccggtat tcatccagga gtgaagcaac agcaggtcgc ggcgtatatt ttggcggctc
      841 agattttgcc tgcaaataac gtttaacggt attgcgggag agccccagtt ctctggcaat
      901 cgcccggcta ctcattccct gcttgtgcag gattttaatt tccataactg tctcaaaagt
      961 gaccataagc tctcctgaat caggagagca gattacccct tggatctgat ttcaggcgtt
     1021 gggtgtggat cactattgca ccgttcgtga cagtgttgtt gcgccttgct gccaacgaag
     1081 ctccactgcc catcaagttc acagataagg gcaacatcag cgtgagcgac tggcgcagat
     1141 gttatccgcc ggggcgggag ctttttaatg tccgtatgac ggtattgatg ccgattttca
     1201 gcgtacggcc agtatcgcga actccggcac cgttgaaagc catttcagtg atttggtttt
     1261 taactcccgg tttacgggcc tcgtaacagt aagtgagctg aaacacccgg tgacagtcac
     1321 ggcaacggaa tcgatcatgt ccttttgggt tctgcccgtg acggtaaacc tgaacggact
     1381 ggcaacgggg gcaatgaaag ttaactgtga ccatgaaaga acctaaaagc gggtattata
     1441 acccctaatt caactaattg gaggcattgt catttttaat ctatggatac acttaaaaat
     1501 cctctcacct tacgttaaaa accttctcat gtttagagtt ttttattatg tttatcataa
     1561 tgatatgacg atctgagttt ttaaaatcaa gaagaaaaga taagtttttt aaatgcacag
     1621 tgcctgtgga taactttgcg taaatccgga ggacagatca ctctgaagca agacaacagc
     1681 atctgaaaac aatatgctca cgtttcaacc acagggcgta agtgatccta cccacgtaat
     1741 atggacacag gcctaagcga ggttctggtt ttcaaattgt tccggactga ggccgccaca
     1801 ccaactgtgc cgtcgccagc gattgtaatc acattcgata taattaaaca ctgttgccct
     1861 cattatttcc cggctgataa agtgttctcc gtggagacat tccactttca gcgaatgaaa
     1921 gaagctttcc acgcaggcat tatcgtagca gcagcccttt gcgctcatac tgccacgcag
     1981 attatgccac ttcagcagcg cctgataatc tgctgaacag tactgacttc ctcggtccga
     2041 gtgaacgata acgttccggg gtctcttacg ccgccacagc gccatctgca gggcatcgca
     2101 ggccagttgc gccgtcaggc gtggtgacat ggaccagcca ataacggcac gtgaccacag
     2161 gtcaatgacc acagccagat acagccagcc ttcatctgta cgtaagtacg tgatgtctcc
     2221 cgcccacttc tggttcggac cactggcgta aaaatcctgc tccagcagat tttctgacac
     2281 aggcaggcca tgtgcgcggt aactgaccgt attgctgggt aagatcaatt tgctcactgg
     2341 tatttactac gtgcatcgaa tgagtgatca agagccgtat gacgcgagag tgtcacgtac
     2401 ggttccgtaa gcagccggag agtgaaactc tctccggctg actcgactcg caggttccga
     2461 cagcggtgac gaacatgcag cggtgttgtt ctcgctgatc ggcacatgcc gtctgaacaa
     2521 tgtggagcca gaaaaatggc tgcgttacgt tattgaacat atccaggact gaccggcaaa
     2581 ccgggtacgc gatctgttgc cttggaaagt tgatctgact tctcagtaaa tatcaatatg
     2641 attctgacga gccgcttacc tttgagcaga gaaaggggag agggatccca catccatgag
     2701 ctaggaaaat acaaaaactg gctatacaac aattcaatat attaggactt acttgcgagg
     2761 agaaatatag caccgctcag gcaattcctg ggtatttact tttcttagtt tctttactcg
     2821 ggatgttctg tcatcagaaa ggcgattcta aatttgtaat atagtcactc atattcccac
     2881 gtaatttctt tagatcaata tattgcacat ttttctcaaa aaactgatcg gaaataagat
     2941 ttgcaacatt gcttgttgta tcatttttaa cgcttctatt catcccttca caaagttcgt
     3001 ccattttctt tttgaaataa ctgcttcctg atacaagatg atttatggaa ctttgtgcag
     3061 cattgatgtc tagagaaatg cgataatctt tctcaacctg tttaaatatc tgtttacgga
     3121 gattatcgcc gatttttcgg ctgtgttctg cacaaaactt atcaatacat tggtttacca
     3181 cccgggatac gtagactttt ttttcatcat ttttccaatc tgtaatattt tcaccgattt
     3241 gttctgaaac agagctcaaa atactagtat ttattttggt tgatgatatt tttactatcc
     3301 ttttccccat tgtacagcgg ataatctcat ccacctttcc tgaaaaatat cttttgtgag
     3361 ataaactgat tccaaaatta ttaaaagatg ttccaagcat tataatcacc ttgtcatgta
     3421 aaatagtaac attgtcatta ttttagctga ttcaaacaat tctgtaaatg ctttgagttc
     3481 tttactccct tggctcaatg atggaccgtc tggaatccca gagactaaac tctctgagaa
     3541 agagagtatg aaaaaacttg gggcagtccc taaatgaagg gaccaacagc ggtaaaataa
     3601 ccggcatcat tcataatgat gccctgttag ttgtagttat gaagatttac gtcattaaaa
     3661 ttgctgttcg tagtgtcagt cctatggtct ggcatcagct gaggatcgta gccgatacat
     3721 cactggcttc acgacattcg tgttgaggct attcatgaaa actccacgct gaaaacgccg
     3781 ttttgtataa acggtcacgg tatgcccgga gccactgttg ctgataaatt cgataaaacc
     3841 ttggcgtttc ttgaagctat cgttaatgcg gataatgaaa caacgattgg tgagattcgc
     3901 tcttttgccg acgccctgga cgctgtccgg ttcaatcgca acaaaatcaa ccggcagctg
     3961 agcaaaccca accttgcctc tctggcgctg gagcatgaag tgatttggct gggacgtagc
     4021 cgctgacagc ttctataaaa gtatgatcct acccacgtaa tatgaacaca ggcttaagtg
     4081 aggttctggt tttcaaattg ttcaggactg agacctccac accaactgtg ccgtcgtcag
     4141 cgattgtaat cgcacttgat ataattaaac actgtatatt tcatttgtgg agtctccagt
     4201 ctgagagggc tttatgcatt ccgtattggg cactatgatg ccagaaatct gcaaggctcc
     4261 acaccttcct gtatttccct tcctctatca cagtgcactt tacttaatag ggtgggatgt
     4321 gttcattaca tttattgtgt tttttatgat tgtttttgcg tccagtgtcg ttcgtcatgg
     4381 gaaactggtt ctatgggtag tatcgctcag tctagttaac tgtttgtttc tttttagaaa
     4441 ttaagcagat cacacaattc ggggagagat ccttctaagt gcaattcttt gcagctattt
     4501 agcacactta aaacaactct tgtattttgt cagaaataat attaaacatg tcttgttcat
     4561 tattgttaag tctgtctgct atttctgtaa gttttatccc actatgatgg catatattta
     4621 tatatgccat catagcctcg atattccctt tccctgcggc cagaaataat cctggtaacc
     4681 cctcattatc tcttacatcc aatagtttaa tcgtttcttt tggtgataaa tcaaaagttt
     4741 taataaaatc tccataagct tgaatcgcag cggaatgacc ttcttgaaga gctatgaaca
     4801 aagcagattc cccagcatta tttcgtgctg caagaacttc tagtttttcc tctttgggta
     4861 tttcggctgc caacagcgat tcaccgtatt tacgcgctgc atcggcattt ccatgccaaa
     4921 tcaatgaaaa caaattatgg cggttatact ccttattatt ctttgtcgga atggatatgt
     4981 tctgtatttt ggccaatttt ctgaaagaaa caccactata tcccccccct gatttttctt
     5041 cattgatggc gtgggatata gcggtatttg tcacgttaat gatattattt ttcattcctt
     5101 tattttcatt agcagtattg ctgcctatta ttaagtgcat atttttgttg tttgctggat
     5161 gtaatccata gttatttatt gacatttttg ttcatccttt ttgctaaaaa aaaacaataa
     5221 cctatttttg taatgtaata gatattagtc tattgctaat ctaaaaaaac cattgccaca
     5281 ttgaatggct atattcacca ataaattctt tcgatttcgt atattgacat atcgtagata
     5341 tatcttatcc ctaagattca ataatcgagt ccgtttacca ttctgtgcaa gcgccttcac
     5401 tcagaagtga ccgtcaaggc gatcaccaaa cttgataata aagcagctca ttgccatacg
     5461 ccagtcctta aggggcgttg tccatttctg tgatgctgcc tagatttaac tgccagctcc
     5521 ggcttatcta catctaaata tgtgagccta ataccgctac actttgtcca gactatgttt
     5581 ttctccggga agtgggctaa ccgtctccat aaagtgacga acttttttca acagtggcca
     5641 tattgaacgg cactgatgat tacgggttat tgtctcatga agagccggcc acatcactga
     5701 cagcagtgac ttcaggcctt cagcaccaga cagcctgaac cagttaatcc atcgcccgac
     5761 aaatttagta atctagggct ggtttttctt cagctgttgc ggttcgaaag tgccattacg
     5821 atcacgcaga gtacggattt ccagaggttc atctccagta gtgataatct tcgttaagca
     5881 accattgcgg gaattgctgc cagatctaag ctgatttttc tcgcacccaa gatgatgagt
     5941 catattagca ttgagtacgg tttcattttc agcatccaat caaactgact gagatcttca
     6001 ggatttttga gacttttggc tagttcatta gctaaagccc gcaacttatt agatgtcaac
     6061 tgtttttcat tcataaattc accttcattt gatatcaaat taaatagttc aacatcagag
     6121 aattatacaa acttatgtac aggctcctat gatcttcgaa tcctgaagat tatagtgaaa
     6181 ataagttttc acgttgaaat cccacatagt tagtatatga taaataatca tggatgacct
     6241 gattaacctc ttggggtagt gcttttatag aagaataaat attctactaa tttaatacat
     6301 cacctaaaat agtattgttt tttatggttg gttgaaggtc gtggtgatga tgttagtcct
     6361 tttgagaata ttccagtgcc agatcttact tataataaag gggcttggca gtttggataa
     6421 actattgttc ttattccact aatacaaact ttttgtaaag tatattaaat atacttcatt
     6481 tcatactgaa atccccggaa gtattcaatc aaacaattac accataaaag gaagaggggg
     6541 ccctaattaa gttgtcaagc atgtttatga tacctgcagg gtataaattt tctggctgca
     6601 agcagacaca tataaaacgt ctttattgat cctacccaca taatatggac acagtcgtaa
     6661 gtgaggctct ggttttcaaa ttgttccgga ctgagaccac cacaccaacg gtgccgtcgc
     6721 cagcgattgt aatcgcactc gatataacta aacactgttg cccgcactat ttcccgatgg
     6781 ataatagtgt tctccgtgga gacattccat tttcagcgaa tgaattttca tctgttgctg
     6841 tatccagtca tacagtgact gcatcagtgg cgcggctctg gcttttcttg ccgccagacg
     6901 ctgttctgct gaacatcccc ggacttctgc ctcgatggca tacagttcac cgatacgctg
     6961 cagggcttcc gtggtgatgt cggttggcac tcttgcatgc acatcgtgga tttttctccg
     7021 gacatgagcc atacatgcgg cttccgttat tctgccggat tcgtacaacg cccggtaacc
     7081 accgtaagca tcggcctgaa gcactccgct gtaaccggcc agatgattct gtggatggat
     7141 acctttccgg tccggtgagt acgcgaacca gaccgccgga ggcatttccg aaccggcgtt
     7201 acggtcatca cgaacgtaga cccacagccg ggcagtcctt gttttaccgc tgcccggctc
     7261 ctggaccgga acggggatat catcggcatg gactttaccg ggcatcagca catactggcg
     7321 caggatgtca tacagcggtt ccagcagttc agcaacagca cctgtccagc gccccagtgt
     7381 ggcacggctc agctccacgc cctgacgacg gtatatttct gactggcggt ataacggcag
     7441 atagtctgca tattttccag caacaacatg ggccagaagc cccgctccgg cataactgcg
     7501 tgcaatgggt tttgaaggca ccggtgcctg cacgatatgg tcacaccggc aacaggccag
     7561 tttcggacgt tgtgtttcga taaccttaaa ggcgctgctg ataagctcca gctgctctga
     7621 cacatcacat cccagagaac tgagttcgcc accacaggcc gggcagcatt cctcttccgg
     7681 acggatgacc cgggtttcac ggggaagtga ggctggtaac ggtttacggg ctgaagactg
     7741 gcgcagggga gatggcagta ccgggtcata ttgctcaccc agcgtttccg ccatttcttc
     7801 ctgaagtgcg ctgattcgct cctgtgcttc cagtatctgc cgttcggttt ttgcgcgaag
     7861 tttttctgag cttttaccga actgcatgcg ctgcagtttc gcaaccagcg ccttcagccg
     7921 gttgatttcg gaagcataag ccgccacccg ctgtgagagc aggcggttgt atttcgccat
     7981 ctggcggatg gtgtcctgtt gcgtctgcaa cagtgcccgc aggcgggcat tctcatgagc
     8041 aagtgaggtg tccatatcct cactttacaa cgggttatat gcggatgcca gcgcgttccg
     8101 ttcgtttcgg gtgtttccag tcgatgccct caagaagcat ggataactgg gccggagtaa
     8161 ggtgcacctt gccgtcacgg gtgaccggcc agacgaagcg gccccgctcc aggcgtctgg
     8221 taaagaggca cagtccgtca ctgtcagccc acaacacttt tatctggtca ccccggcgtc
     8281 cgcggaagat gaacagatgc ccggagaacg ggtcatcctt caggacgttc tgaacttttg
     8341 agaccaggcc gttaaagcca tttcgcatat cggtgatacc tgcaaccagc cagatacgcg
     8401 aacctgcagg gaaagatatc atcagtggct gctccctttt atttcgcgga taagtatctg
     8461 taataacgcc ggcgtcagtt tacctttaag cctgagagtt ccggccggca gaaccagctc
     8521 acagcacaga ctgtcggacg gtgtatttat ctgctctggt tcctgtgccg gagccgggat
     8581 tttattatcc ggctccggcg ttaacgtcac gggaagcagt gcaggcatat tttttccgga
     8641 aggcagcagg ccacctttcc ggtattgatg gcgccagttg aagagcaggt tatcgttgat
     8701 tccgttttca cgggcgatct gcgccacaca ggctccgggc tgcagtgact gctccactaa
     8761 agcgatttta aattcataag gaaaattagg ccgc
//
LOCUS       DAZXUL010000155         8768 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZXUL010000155 DAZXUL010000000
VERSION     DAZXUL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8768
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.155"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..439
                     /locus_tag="ACH3L1_003745"
     CDS             <1..439
                     /locus_tag="ACH3L1_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFT4078315.1"
                     /translation="QKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQ
                     FAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYA
                     KAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACH3L1_003746"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACH3L1_003746"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFT4078316.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKNSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACH3L1_003747"
     CDS             complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACH3L1_003747"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFT4078317.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACH3L1_003748"
     CDS             complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACH3L1_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFT4078318.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4441..5148)
                     /locus_tag="ACH3L1_003749"
     CDS             complement(4441..5148)
                     /locus_tag="ACH3L1_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFT4078319.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACH3L1_003750"
     CDS             complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACH3L1_003750"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFT4078320.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6006..6209)
                     /locus_tag="ACH3L1_003751"
     CDS             complement(6006..6209)
                     /locus_tag="ACH3L1_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFT4078321.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6368..7876
                     /locus_tag="ACH3L1_003752"
                     /pseudo
     CDS             6368..7876
                     /locus_tag="ACH3L1_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7873..>8768
                     /locus_tag="ACH3L1_003753"
     CDS             7873..>8768
                     /locus_tag="ACH3L1_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFT4078322.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQ"
BASE COUNT         2036 a         2388 c         2252 g         2092 t
ORIGIN      
        1 gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg
       61 cgtgttttat gtcgcgggag ataaagtcgg catcgttcgt ctggatgctt ttaaagccag
      121 taaagatatt cagtttgcgg tgcagtcagg accaatgttg atggaaaacg gtgtaattaa
      181 tccgcgtatt catcccaacg tcgcctcacg taaaattcgt aacggtgttg ggattaataa
      241 acatgggaat gccgtgtttt tgttgagcca gcaggcgaca aatttttatg attttgcctg
      301 ttatgccaaa gcgaaactga acgttgagca gctgctttat cttggtggca cgatttcaca
      361 tatgtatatg aaaggcgggg caatcccgtg gcaacgctat ccttttgtca ccatgatttc
      421 cgtcgagcgg aaaggttaga atgatagctt tgtgtcagat actaaatatg ctgctgatgc
      481 cggatgcggc gtaaacgcct tatccggcct acatgacgtt gcaatttatt gaatctgaat
      541 gattttttag gccaaataag gtgcgcagca ccgcatccgg caacgttaca ccgactccag
      601 ccgggcgtaa gctgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg
      661 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      721 tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg
      781 atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg
      841 caactcgcca ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
      901 ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
      961 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1021 ttccataccg acgataaaca cctgcgggaa ctccagacct ttcgccgagt gcatggtcat
     1081 cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg
     1141 ggataggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg
     1201 cgttgccgtc accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1261 ctgctcgtac atggtacgca ggccggagtt tttaattacc cggtcagtct gtacatgcag
     1321 cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc
     1381 gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag
     1441 tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1501 ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag
     1561 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccgccgta
     1621 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacacgcg actgggcgtt
     1681 actgcggtag agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat
     1741 gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg
     1801 ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttat tgttttcaat
     1861 cagggcgtta gcggcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     1921 aatttcggca ccggggaaat cattaaggaa acgctgaata ttctccacct gcgccccgcg
     1981 ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc
     2041 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2101 attggtaaaa cgttcgcggt agtgttgtag gatatgcggc ttgttaagcc acagttcgtg
     2161 agcgcgcagc agcagctcgg cgaagtccac caggcccgcg cgatcgcacg cttcctgata
     2221 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2281 cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2341 ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc
     2401 gctatcgagg atctggaaat cctgcggcag attggcgtcc atatggtgcg cacgcagcag
     2461 gcggtgcgcc agcccgtgga aggtgcctac ccacatgccg ccctggctgg tgcccatcag
     2521 ttgtccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat
     2581 cgagtatggc gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg
     2641 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2701 ttcgcgttgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2761 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     2821 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgtggc
     2881 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc
     2941 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3001 atctcgccga tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt
     3061 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3121 agttccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3181 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta
     3241 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3301 gcttgttcaa tcgaacgaaa acgccaacgt gtcacgtcgt gataaatctc gggttccgct
     3361 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3421 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3481 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3541 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3601 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagtagct
     3661 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3721 atttatgtgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3781 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     3841 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     3901 caatcggcag gcggcgctct ttgctgcctt tccccatcac ccacacttcg ccggactcca
     3961 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca
     4021 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4081 gccgattcat atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg
     4141 aaactccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4201 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4261 gtacagcaaa attgcgtacc atcgctgcat cgcattgttg ccagctttgc aggccgtttt
     4321 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagtagg gttatcgggc
     4381 taagctgacg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4441 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4501 taactgcgtt ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacaac
     4561 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4621 cggtagcacc accagcagct ccggtccgtt aagcggccca agatagtgct gttcctgccc
     4681 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4741 cgacggcgca cctaagcgcc agcgatctgg aaacaggcgc agactcgcac cagctaggcc
     4801 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     4861 gagactgcgc tgcaggtaga gtaggcgata aaacagccct tcgttggcga tagcctgctc
     4921 catcaacaac gccatgttct cttccagaac atgaatgtga ttacgtgcgc gggccatgtg
     4981 ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc
     5041 gcgcgcattg cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc
     5101 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5161 ccatcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5221 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcgtccag caaaccttgc
     5281 tgaatcccta ccgccaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5341 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5401 ttggcgcgct ccggaaaacg ctcgtgactt tccagaacag gaccaagcgt ttctaccgcc
     5461 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5521 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5581 aacggcacgg cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca
     5641 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg
     5701 gtcagtcctt tcagacgcac aaaacgggca aagcagcgcg caccgttacc gcactgcgcc
     5761 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     5821 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc caggcgacga
     5881 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     5941 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6001 gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc
     6061 accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca
     6121 ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat
     6181 acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct
     6241 gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc
     6301 tgaaaagtga ggaaatgctg cgagtataac gcttactcgt gaggatttat gttctgctgg
     6361 cagtttcgtg atcaacacct cgcgccagga tggctttatg cactatcgca atgtggtttg
     6421 tttgttgtct tgttcattgt ttttatcgtc ggcatgggga tgtcggcttt ccgcacctga
     6481 acataatatt taccagaaac agggaaaagg cgtggtgtac ctgcgccctt ataaagaaac
     6541 aaatctttct ctacctgacg tgaattacaa gagtttgcgt cggttaccca atttattaat
     6601 agacccgaca acactagacg aatgggataa agaaccgcct ctgactgatt tgacaaccga
     6661 ctacctctat gagggcgcac aggcctggta tcctcattat tcatggcata gcgatggtcg
     6721 caatatcctc tacgcgggtg aagttgtgca aaaccctccc ggcacacctc ccgtcgatgt
     6781 cgcctcgttc aaggcatggg gtgattttgc cgcagataaa cacagtctct attttgaagg
     6841 caagcgcacc gatgacaatg gtggtggaaa tagcttggat atcaaaacgc tgcatcaggt
     6901 ggaattccgg cctccctggg atcctgactt actggggctg atattacgtg acgctaattt
     6961 cttgtatatc aatgggcatc gcctcgctga ccccgagagt ttccgtgtgc tggcgcagaa
     7021 gtcctgggat cagcgaggaa agttttccac cacgtttaac ccctgcattg ccgtaccatt
     7081 tggcccctgg gataccctgg ctcgtacacg gaccaaaatc ctgctcaacg gcgaacagct
     7141 tgatgccgac ccggacacct tttccgtcgt gcgctggatg cccggctcac tcttgacctg
     7201 gcgtgataaa aacgggctgc agcgtaaagt cctcgacaag gaaaatctgg cgtgggatga
     7261 agatttaaca aagcactgtc tggatttttc tctgctggaa aagaaagtgt tctggcgtaa
     7321 aggacctgct tgtaaacagg aagaattacc cggactcgat ccggagcagt ttcaacccat
     7381 cagtgatgct gtcgcccagc atcaggactc gctttatacc atcatcgaaa cagagtctgg
     7441 taaccgtaag ctggagatcg tgaaacttga tgatcccaat cttattatca acaaacgttt
     7501 caacgccggg aaacgccacg gctatttact tacgcgtgcc gaagggtagc cataccattc
     7561 cggtttacac gtgtttgaat ctgacggacc gctgatctta ctggataacc gttctccgga
     7621 tgaacgcgaa gcccatctta atgaccatcc ctttttgcgc agatggtatg cccgcgataa
     7681 ccgctacatt tacagctttg atggcgcgca gctctggcga taccgcaccg ctgatccgaa
     7741 acaagttcgc ttaatctgga aggaacaaca ttcgggatat gtctatggcg taaattacaa
     7801 aacgggatat ctggacggaa aaattactga tgacggcgaa tttattcctg ccccgcgcaa
     7861 tgaggcgaca aaatgaaggg tcaaataatc acagttgcac tcatcctgtt gggacacaca
     7921 tttctgacgc ctgctgtaca ggccataggg ttcgactatt acaatgatca cggtgttatg
     7981 tcttacggta aaggttattg gggggataag aaaatcattg cccagtttcc ggagatgagc
     8041 agggccggtt tgcgccttgt gaaaaatatc tctgggaaaa cgggttatat accttccgat
     8101 gaaataagca tagaaaatga ggaataccac tgggtgacag acggccgcgt tattctctgg
     8161 cgtggcaaaa tcgttagcaa tccaccggga acactcactg tcgatattgc cagctttcag
     8221 gctatgggcc gcttcgcggt cgataaatat agcctctatt ttgacggaca gcgcaccgaa
     8281 agtaatagcg gcgcgtcacg tgttgagctg gcgacactaa aagctatcga aggtaactct
     8341 accacgctgg tggatagtaa aaatctctac ttgtccggtc gacgtcaggg gagcagcagt
     8401 gatgttactg tattagaaaa aagatggtgg ggtattaatc cacgtcttat gagtgtaaac
     8461 agaaattcat attccaatga tctgcttatc cgcagtgggc agaatattta tttaaatggc
     8521 gttcacctta cggcgaatgc agactcattt gagataattc gctggatacc tcattcactg
     8581 ctggtttttc gcgacaataa gggtctgcat cgttatccct ttggtcaatt atcaggcaaa
     8641 gcgatacccg tagatgatga cgtctctttt gaagtagggg aaagtcgcgt tcgctggcgt
     8701 aaacagctca cgcccgaccg tcagtggagc aagtggatag acctaccagg tattgaacct
     8761 gaacaatt
//
LOCUS       DAZXUL010000156         8727 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZXUL010000156 DAZXUL010000000
VERSION     DAZXUL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.156"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(96..1358)
                     /locus_tag="ACH3L1_003754"
                     /pseudo
     CDS             complement(96..1358)
                     /locus_tag="ACH3L1_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1520..1595)
                     /locus_tag="ACH3L1_003755"
     tRNA            complement(1520..1595)
                     /locus_tag="ACH3L1_003755"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1560..1562),aa:Asn,seq:gtt)
     gene            complement(1696..2493)
                     /gene="mtfA"
                     /locus_tag="ACH3L1_003756"
     CDS             complement(1696..2493)
                     /gene="mtfA"
                     /locus_tag="ACH3L1_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFT4078323.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2587..2676
                     /locus_tag="ACH3L1_003757"
     tRNA            2587..2676
                     /locus_tag="ACH3L1_003757"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2621..2623,aa:Ser,seq:cga)
     gene            complement(2704..3754)
                     /locus_tag="ACH3L1_003758"
                     /pseudo
     CDS             complement(2704..3754)
                     /locus_tag="ACH3L1_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3754..3894)
                     /locus_tag="ACH3L1_003759"
                     /pseudo
     CDS             complement(3754..3894)
                     /locus_tag="ACH3L1_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            3921..4337
                     /locus_tag="ACH3L1_003760"
     CDS             3921..4337
                     /locus_tag="ACH3L1_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078324.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            4989..5144
                     /gene="hokD"
                     /locus_tag="ACH3L1_003761"
     CDS             4989..5144
                     /gene="hokD"
                     /locus_tag="ACH3L1_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFT4078325.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            5312..5590
                     /locus_tag="ACH3L1_003762"
     CDS             5312..5590
                     /locus_tag="ACH3L1_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078326.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            5592..6650
                     /locus_tag="ACH3L1_003763"
     CDS             5592..6650
                     /locus_tag="ACH3L1_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFT4078327.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            6651..7016
                     /locus_tag="ACH3L1_003764"
     CDS             6651..7016
                     /locus_tag="ACH3L1_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFT4078328.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            7013..7701
                     /locus_tag="ACH3L1_003765"
                     /pseudo
     CDS             7013..7701
                     /locus_tag="ACH3L1_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            7895..7970
                     /locus_tag="ACH3L1_003766"
     tRNA            7895..7970
                     /locus_tag="ACH3L1_003766"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7928..7930,aa:Ile,seq:cat)
     gene            7972..8048
                     /locus_tag="ACH3L1_003767"
     tRNA            7972..8048
                     /locus_tag="ACH3L1_003767"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8006..8008,aa:Lys,seq:ttt)
     gene            8051..8126
                     /locus_tag="ACH3L1_003768"
     tRNA            8051..8126
                     /locus_tag="ACH3L1_003768"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8084..8086,aa:Thr,seq:tgt)
     gene            8130..8204
                     /locus_tag="ACH3L1_003769"
     tRNA            8130..8204
                     /locus_tag="ACH3L1_003769"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8163..8165,aa:Gly,seq:tcc)
     gene            8497..8712
                     /locus_tag="ACH3L1_003770"
     CDS             8497..8712
                     /locus_tag="ACH3L1_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFT4078329.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         2258 a         2005 c         2177 g         2287 t
ORIGIN      
        1 aggtgttttt cgtcatgaag ttgataaaag tctatataga accacctcct gtagaggtgg
       61 tggtaatttg ccagagtgtg ggattcgtta gtatactatg ggataaactt atttttacca
      121 attgtataag gcggtgcata agattctcgg catgcttcca gataatccga ccaccactgc
      181 atcatcgctt tgcgggcttc caggtgctct gccttatgaa tataagccat gcgcacggta
      241 ttgcgctcct gatgactcat ctggcgttct actgcgtcct ttgcccataa acccgattcc
      301 atcagcgcac tgcatgccat tgcccggaag ccgtgaccgc agatatcctt tttcgtgtcg
      361 taacccatca cccgcagtgc cttattgacc gtgttttcac acattggctt atacgggtta
      421 tggtcgccgg ggaagatcag tttattatta ccggtgatat ccttaatctg tttcagaatg
      481 gcgatggact gttctgagag ggggacgata tgcggcattc gcatttttgc cccgcggccg
      541 gaataacgca cgccaataat gggttctcgc gtcgcgggta tcgtccagac tcggtttgtg
      601 aaatcaatct ctgaccagcg ggcgaaacgc agttcactgg agcgaatgaa cacatgcagc
      661 atcagcagaa cggcatgccg ggtcagttca cggccctgat gatatgcccc aatacgttca
      721 agcagttcag gcagccgctc cagcggcagg gcaggatagt gccgtctgac aggaggtgtg
      781 gtcacgccgc caaggtttgc tgcagggttc gtatcgatta actcctgatg gaccgcatgg
      841 cgcattatgt tactcaggtg ctgccgtgtg cgggacgcaa cctccagcag acctttttcc
      901 tcgatccctt tcagcaggtc aatgaaatga cggggtttaa gttctgatac aggtaggttc
      961 ccgatgaccg gaaagatgtg attgttcagg ctggcaagca gacggtcggc ggtgttctgc
     1021 gaccatttcc tgttactttt atgtcacgcc agcgccatgt ttttaaaaac tttctccggt
     1081 gttcgtgagc cacgttcagc agcccgctgc tgtaccgggt tgatattcag cgccagcatt
     1141 ttacggatac cttcacgttg ctgtcgcgca tcagacaggg agatggctgg ataggcaccc
     1201 agcgcaatgc gggattcttt accgctaata cggtatttga gataccagtg gcgggagcca
     1261 cccggcttga ccagcagata cagaccgtga gaatcggaga ctttaaaggg tttatcagaa
     1321 ggcttgaggg tgcggatttt tgcgtcggta agggatatat gggggtcact ccattatcga
     1381 actaacctga tcccagattt gaccaccaat ttttcccgat gcgtagggta aaatcaaaat
     1441 gcaccgagaa gagttttcac actaacctgt tgaatcaaca tcagataaag attcgcaagg
     1501 atgcatgaaa acagaaaatt ggctcctctg actggactcg aaccagtgac atacggatta
     1561 acagtccgcc gttctaccga ctgaactaca gaggaatcgt gagaacgggg cgcatattac
     1621 ttagcggtac cttgtctgtc aacactaaat ttcataagcc atttcaattg gttaattaat
     1681 cttcaaagtt gttaattaat gaacattcgt cgctgaaaac gagtctgtat cattagcgtg
     1741 atgcagtctc tgcaaaggat cttgttgata aaattgacag aaacgttgcc acaatgaagg
     1801 gaaacgagga gcaaaaagtt ctggggcgct aaagaaatat tcagaaagta cggcaaaaca
     1861 ttcagcagga tcactggcag cataagcatc aatgctcgcc gcattctcac caactaattc
     1921 gatttcttcc tgaatgttgt tcattgcagc atgaagatcg tgttcccagc cagcaacctc
     1981 acgcaacgaa ataaagggaa ctccgctggc gcgatcgccg ttacgggtgt ccagcttatg
     2041 agcgacttca tgaataatca ggttaaaacc agaagcatca aaagaatctt gtatatccaa
     2101 ccagttcaaa acgataggcc cttgctgcca gctctgacct gactgaacaa tacgttggtt
     2161 atgcaccaga ccgatatcgt cttcccattc atcatcgacc acaaatggcg caggataaat
     2221 taagacttca tgaaaaccat ccagccattc cagtcctaac tccagaacgg gcaggcaaaa
     2281 tagaagtgct atccggcagc ttcttaatga attcagctca aagccctgta aaggaacaag
     2341 ccgtttttgc tgtaaaaaac gttcggcaag agcgaccaat ttgctttgtt cctgttctgt
     2401 cagacacgtt aaaaggggga tcgatagtgc ttcctgccag ggaagggcag tttgatgtgc
     2461 tgattcttgt actttccagg gccacttaat catcgttttg ctcgcaaact cgtcacttga
     2521 acaaaattgc acggacaggg actgttaaaa tgccaaattt cctggcatca tggcaaccat
     2581 ctgaacggag agatgccgga gcggctgaac ggaccggtct cgaaaaccgg agtgggggca
     2641 actccaccgg gggttcaaat ccccctctct ccgccaaaat tcaatcactt atacatcatt
     2701 aagtcagtga caaaaatcac acttggaatt acttggaata ttttcttgga atattttcag
     2761 gtaacgggac atcaagtgtc ggtgaaactt taaccttcct gtcatagatt agcacttgcc
     2821 cctcggtttt gtgaccagag aaaagttgct tatcccgact gcttccttca tagtctgaaa
     2881 ttcctttcgc cttcagatca tgaaaggtga agtcggttaa aatacctgaa attttgcctg
     2941 cgcgatttct tgcttctacc cacatttcgt taaagccttt gtacatatat cggttgccgt
     3001 attgattgct gattacatag gcggatgttg gtaactgttt tgctttttcg atcgccgcct
     3061 gtaatcgtgg actccatgct tttatctgtt tttttcctgt tttcccttgc tggataaaga
     3121 tcccgtcgtt tccaatctgc tcccatttca gcgataacac atcggaaacc ctcgctgcac
     3181 acagataggc aatttccatt gcgataaaaa caggaagagg tgcaacgctt aatactgcct
     3241 ggtattcttt gtcggttaca tatcgttcgc ggtttttggc cttgaattta cttacacctg
     3301 cacatgggtt agccttcacg taccctcgct cataccccca actgtaaacg cgggacatac
     3361 tgcttttttc atggttggct tgcgttttac tctgttcccc tctcttgtcc atgtatcgac
     3421 ggatgtgttc tggttttatg gaatctgccg gcaccttacc gaatacggca agcaactttt
     3481 tttgatgttg cagataatct ttttgtgttc ttggactgag gtcactgtaa taggcgctgg
     3541 cgaggaattt ttcccacaag cgaccgaatg tcattgcacg atcgcgatta tttacagttt
     3601 cctcatactt tttccataaa gcagctaaac catccttgat ggcggttagt gttacagatt
     3661 ctctggatgt tggtttccat acataactat atttatttgg gtatacattt ggaggtaatt
     3721 tttcgtgttc aggatttttc cttcgtcttc ccatcagatc gcaccaaaat tcggctctac
     3781 ctcgcgtggt ggtaaagttt tattgcaggt aaatagatcc cggctgacaa tcggtttgcc
     3841 actacgattg gtatagaacg gaagcccgtt ttccattaac cattttcgct ggtggcagca
     3901 ttaccagggc ggtaatgaaa atgactgaat taacaaaaga acaattaatc gaagaagcca
     3961 aattaaaaat agcgattacg aaatgccacc ccaattcagg gatggcgcga gtagagggcg
     4021 agttattcaa aattgcacgg gcatcgctgg aagcagagcc gatagcgtgg cgatatcgct
     4081 acgtgaaaaa aggtgttatg gactctcagg gggagttgtg ggttggtgac tggaaatatg
     4141 taccgaaaaa agaggattgt aacgacaggc cgaactatga aattcaggcc ttattcactg
     4201 ccccaccagt cccggttaca tcagaagaac tggttaaagc tgtgcacttt tatgaacaat
     4261 taaaacgcga aaatccacca gcatccggca accagattaa tgggttaact atgtcggtta
     4321 aacgaccagc caactgaaaa agcggaaacc tgattatagg ttgccagata aggcaataag
     4381 ctacctggcg cggaacggac tgataagtat ggggaatgtt ttacgatgag tatttagact
     4441 aaagagtttg taacgctatg taagtgattt tttctggttt agatatttat atgtccggcc
     4501 aaattgaggt gtgtttaaat gttattgcac attgattgta gggggaataa tgaaaaacgc
     4561 attgcagttt ttgtttgttg cgttctggtt gttcgcatca tgtatgccca tcatcttcac
     4621 agcaaggtat atggaaaaaa ttgatgtttt gatattaatg tttggacata taaatgccct
     4681 ttttttaggg gtgttcatgg cggtcatgtg cattgaatac tggcggtaaa tacagcgaac
     4741 gctattggtt tagttggata tttactgtgc cggacaaaaa cggtttgcag ggaaatctta
     4801 gttaagtaga atgactgcgg gtgcttgagg ctatctgtct caggcatgaa caccaaaagg
     4861 cagatagaga aaagccccag ttaacattac gcgtcctgca agacgcttaa cattaatctg
     4921 aggctcaatc catgctgaac acatgtaggt tagcctctta cgtgccgaaa ggcaaggaga
     4981 agcaggctat gaagcagcaa aaggcgatgt taatcgccct tatcgtcatc tgtttaaccg
     5041 tcatagtgac ggcactggta acgaggaaag acctctgcga ggtacgaatc cgaaccggcc
     5101 agacggaggt cgctgtcttc acagcttacg aacctgagga gtaagagacc cggcggggga
     5161 gaaatccctc gccaccgctg atgtgtcagg catcctcaac gcacccgcac ttaacccgct
     5221 tcggcgggtt ttgttttttc tggtcgttct ggtttacaat ccatccgtca gcctgaacaa
     5281 ctggcacctg ctgcgccagc agagaaaaca gatggcgcac gataccaaat tttacaattc
     5341 ggataactct gccgcccctg ccagcaggca cgggcggcgt tctcatgcat tcaaatctga
     5401 cgggtatcag cacgacccct gcaccgaaga acaggctgaa tggctgattc agcgctaccg
     5461 cagacacgga tacgagatta agaaagccct cagtctcgat tatcgtcact ggataatctt
     5521 cgtcaggctc ccttattccg aacggccacc gcgtccgtcc tgcacattcc agcaacggat
     5581 ctggaggtaa tgtgcgggta ttacttcgac ctgttctggt tccggaactc gggctggtgg
     5641 tccttaagcc cggtcgtgaa tccatgccgg tattccacaa tacccgggta ctggtggagc
     5701 cggaaccgaa aagcatgcgt aatctgccgt ccggggtcgt tcctgccgtt cgccagccgc
     5761 tagtggaaga caaaacattg ctgccgtttt tcagtaacgc acgggtgatt cgtgctgctg
     5821 gtggtgctgg cgcattgtct gactggctgt tgcgccatat taaatcctgc cagtggccac
     5881 acggcgatta tcatcacagc gaaaccgtca ttcaccgtta tggtaccggc gcaatggtat
     5941 tgtgctggca ctgcgacaac cagctgcgtg accagacatc cgaatcactc gagcaacttg
     6001 ctcatcaaaa cctgtcagca tggatgattg acgtcatcgg tcacgcaata agcggtacgc
     6061 aggagcgtga attatctctg gctgaattat cctggtgggc ggtccgcaat caggtggcgg
     6121 acgcgctacc ggaagcggta ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct
     6181 caatgtaccg tgaaagcgac atcgtgccgg gagagcagac cgccaccagc atactgaagc
     6241 agcgcacaaa aaatcttgcg ccgctgcctc acgcccacca gcaacagaat ccaccacagg
     6301 aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat ctccagcgcc
     6361 agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca
     6421 tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga ctgggaggga
     6481 tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac
     6541 tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg ttgctgattc
     6601 gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa atgaccgaac
     6661 gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga cgtcgtggca
     6721 gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg ctaattgttc
     6781 gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt attgcagagc
     6841 caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg gatgcgctga
     6901 cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt gtgcgcggtc
     6961 agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc gcatgaataa
     7021 ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt tgagtggggc
     7081 aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag ggcgttacag
     7141 gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc gggataatcc
     7201 accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg tcagtccggt
     7261 tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc actataaagc
     7321 ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg cgataacaca
     7381 gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag ggaaaacgca
     7441 ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag aactttttgg
     7501 tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa ctaaaaactg
     7561 gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc gactggatag
     7621 tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa
     7681 agtgttgcaa aagtagattg aaaggcatat atttatgcaa atctgatact ttgccgattt
     7741 tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg tgccacttat
     7801 ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt gaatgcttgc
     7861 aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt ggtgagagcg
     7921 agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca accgccacta
     7981 gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga gtccccggtg
     8041 gcggtccagt gccgacttgg ctcagtaggt agagcaactg acttgtaatc agtaggtcac
     8101 cagttcgatt ccggtagtcg gcaccatatg cgggcatcgt ataatggcta ttacctcagc
     8161 cttccaagct gatgatgcgg gttcgattcc cgctgcccgc tccagtcaga gtctttcagt
     8221 ctgcgatgat gggaaatccc ggagtgactg aaagacgttt aagttatgaa tgatcgcttt
     8281 tttttgcaaa attgctgtgc agaaatacta accttcgggc aggcgatcat tcataagcac
     8341 tctgctttta ttccgattaa ctgtgagtgg tttgttggat agagtgcttt ccttattgta
     8401 tatatcgttt cgcccgcttt tgcggttttt tcttttcaaa tccctttcat ttctcagtgt
     8461 aaaactacgc catccgttat ttgcggaggt gaggctatga aatccatgga caaaatttca
     8521 acgggcattg cctacggcac ctccgcaggc agtgctggct actggttttt acagtggctt
     8581 gatcaggtca gtccgtcaca gtgggctgcg attggtgtac tggggagtct ggttctgggc
     8641 ttcctgactt atctgacaaa tctgtacttc aaaatcagag aagacaagcg taaggctgcg
     8701 agaggtgaat aatgtcgcca tcattac
//
LOCUS       DAZXUL010000157         8693 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZXUL010000157 DAZXUL010000000
VERSION     DAZXUL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.157"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACH3L1_003771"
     CDS             complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACH3L1_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFT4078330.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            696..914
                     /locus_tag="ACH3L1_003772"
     CDS             696..914
                     /locus_tag="ACH3L1_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFT4078331.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            922..1863
                     /gene="ribF"
                     /locus_tag="ACH3L1_003773"
     CDS             922..1863
                     /gene="ribF"
                     /locus_tag="ACH3L1_003773"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFT4078332.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1906..4722
                     /gene="ileS"
                     /locus_tag="ACH3L1_003774"
     CDS             1906..4722
                     /gene="ileS"
                     /locus_tag="ACH3L1_003774"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFT4078333.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4722..5216
                     /gene="lspA"
                     /locus_tag="ACH3L1_003775"
     CDS             4722..5216
                     /gene="lspA"
                     /locus_tag="ACH3L1_003775"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFT4078334.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5303..5752
                     /gene="fkpB"
                     /locus_tag="ACH3L1_003776"
     CDS             5303..5752
                     /gene="fkpB"
                     /locus_tag="ACH3L1_003776"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFT4078335.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5754..6704
                     /gene="ispH"
                     /locus_tag="ACH3L1_003777"
                     /gene_synonym="lytB"
     CDS             5754..6704
                     /gene="ispH"
                     /locus_tag="ACH3L1_003777"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFT4078336.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6770..7661
                     /gene="rihC"
                     /locus_tag="ACH3L1_003778"
                     /pseudo
     CDS             6770..7661
                     /gene="rihC"
                     /locus_tag="ACH3L1_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
     gene            7828..8649
                     /gene="dapB"
                     /locus_tag="ACH3L1_003779"
     CDS             7828..8649
                     /gene="dapB"
                     /locus_tag="ACH3L1_003779"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFT4078337.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         2000 a         2155 c         2458 g         2080 t
ORIGIN      
        1 aggtctaatg aggagatatt tatgaatggt taaagtatat aggaatttaa ttcaaaaata
       61 gagtggtctt aagtttaata cctcaatgtg tatcacaata tccatattct ttgtggggga
      121 gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct
      181 taagccacga agagttcaga tagtacaacg gcatgtctct tttgactatc tggcaaccgg
      241 cagtgtgttc tctcacgcat cacaaaagca gcaggcataa aaaaacccgc ttgcgcgggc
      301 tttttcacaa agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg
      361 ttagccttat gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc
      421 acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata
      481 gctgcgtata ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta
      541 cgagcctttt cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa
      601 ctcccaaatg tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc
      661 ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg
      721 atggcgcagg attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg
      781 cagcaggcgg tcagtttctt cccgtgattt gctccatggc aatgaaaagc cacttctttc
      841 tgatttcggt actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat
      901 tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc
      961 ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc
     1021 gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg
     1081 ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc
     1141 cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg
     1201 cgtttcgaca ggcgttttgc ggcgttaacc gcgcaaaatt tcatcagcga tcttttggtg
     1261 aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt
     1321 gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga tatcaccagt
     1381 acgcaaactt tttgcgaagg tggtgtgcgt atcagcagca ccgccgtgcg tcaggcgctg
     1441 gctgatgaca atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg
     1501 cgtgtagtcc acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg
     1561 ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt
     1621 gaaaagccgt tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc
     1681 cagcagctgg aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa
     1741 gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa
     1801 gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct
     1861 taagcctgtt atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa
     1921 tcaaccctga atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa
     1981 ccgggaatgc tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa
     2041 aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat
     2101 attggtcact cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc
     2161 ggttatgact cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa
     2221 gtagagcaag aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag
     2281 tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc
     2341 gtgctgggcg actggtcgca cccgtatctg actatggact tcaaaactga agccaacatc
     2401 atccgcgcgc tgggcaaaat catcggcaat ggtcacctgc acaaaggcgc gaagccggtg
     2461 cactggtgcg tagactgccg ttctgcactg gcagaagcgg aagttgagta ttacgacaaa
     2521 acttctccgt ccattgacgt cgctttccag gcggtcgatc aggatgcgct gaaagcgaaa
     2581 tttggcgtaa gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg
     2641 actctgcctg cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag
     2701 atcgacggcc aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc
     2761 ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg cagagcttga gctgctgcgc
     2821 tttacccatc cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg
     2881 gatgccggta ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc
     2941 ggtcagaaat acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg
     3001 ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg
     3061 ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc
     3121 tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacac cgcagtggtt cgtcagcatg
     3181 gatcagaaag gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggctcccg
     3241 gactggggcc aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc
     3301 cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg
     3361 catccgcgta ccctcgaact gatggaagaa gtggctaaac gcgttgaagt tgacggcatc
     3421 caggcgtggt gggatctcga tgcgaaagag atcctcggcg acgaagctga ccagtatgtg
     3481 aaagtgccgg atactctcga cgtatggttt gactccggat ctacccactc ttctgttgtt
     3541 gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag
     3601 caccgtggtt ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg
     3661 tatcgtcagg tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa
     3721 tccatcggca acaccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg
     3781 cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg
     3841 aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac
     3901 ctgaatggtt ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat
     3961 cgctgggccg taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca
     4021 tacgatttcc acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc
     4081 ttctacctcg acatcatcaa agaccgtcag tacaccgcca aagcggatag cgtggcgcgt
     4141 cgtagctgcc agactgcgct gtatcacatc gctgaagcgc tggtgcgctg gatggcacca
     4201 atcctctcct tcaccgctga tgaagtgtgg ggctatctgc cgggcgaacg tgaaaaatac
     4261 gtcttcaccg gtgagtggta cgaaggcctg tttggtctgg cagacagtga agcaatgaac
     4321 gatgcgttct gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa
     4381 gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa
     4441 ccggaactgg cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc
     4501 tccggcgcta ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta
     4561 ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg
     4621 cactacaccc aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc
     4681 agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt
     4741 caacagggct acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca
     4801 aatacctgat cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta
     4861 atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct
     4921 ggcagcgttg gttctttgcc ggtattgcga ttggtattag cgtgctcctg gcagtgatga
     4981 tgtatcgctc gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg
     5041 gcgcgctggg caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact
     5101 tctacgtcgg cgactggcac ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg
     5161 gtgcggcact gatcgtgctg gaagggtttt taccttccaa agcgaaaaaa caataataaa
     5221 tcctgccgga tgcggcctac agattgctac gaaatcgtag gccgcatccg gcaaaaatcc
     5281 tcaaaaataa gagcaaacct gcatgtctga atctgtacag agcaatagcg ccgtcctggt
     5341 gcacttcacg ctaaaactcg acgatggcac caccgccgag tctactcgca acaacggtaa
     5401 accggcgctg ttccgcctgg gcgatgcttc tctttctgaa gggctggagc aacacctgct
     5461 ggggctgaaa gtgggcgaca aaaccacctt ctcgctggag ccagatgcgg cgtttggcgt
     5521 gccgtcaccg gacctgattc agtacttctc ccgccgtgaa tttatggatg caggcgagcc
     5581 agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc ctggtgtgat
     5641 ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg ccgggcagac
     5701 cgttcatttt gatattgaag tgctggaaat cgatccggca ctggaggcgt aacatgcaga
     5761 tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc agcattgttg
     5821 aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg gtgcataacc
     5881 gctacgtggt cgatagcttg cgcgagcgtg gggctatctt tattgagcag attagcgaag
     5941 tgccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg gttcgtaacg
     6001 aagcgaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg accaaagtgc
     6061 atatggaagt tgcccgcgcc agtcgccgtg gcgaagaatc tattctcatc ggccacgccg
     6121 ggcacccgga agtggaaggg acgatggggc agtacagcaa ccccgaaggg ggaatgtatc
     6181 tagtcgaatc gccagacgat gtgtggaaac tgacggtcaa aaacgaagag aagctctcct
     6241 ttatgaccca aaccacgctg tcggtggatg acacgtctga tgtgatcgac gcgctgcgta
     6301 aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc acgactaacc
     6361 gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg gtcggttcga
     6421 aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc aaacacgcgt
     6481 ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt aaatgcgtcg
     6541 gcgtgactgc gggcgcatcg gctccggata ttctggtgca gaatgtggtg gcacgtttgc
     6601 agcagttggg tggtggtgaa gccattccgc tggaaggccg tgaagaaaat attgttttcg
     6661 aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt agcagcctaa
     6721 gttatgcgaa aatgccggtc ttgttaccgg cattttttat gaagaaaaca tgcgtttacc
     6781 tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg ccgcgatttt
     6841 tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct cggttgagaa
     6901 aactacccgc aatgccctgc aactgctgca tttctggaat gcggagattc cgctcgccca
     6961 gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc acggcgaatc
     7021 gggaatggct ggctacgact ttgttgagca caaccgaaag ccactcggga taccggcgtt
     7081 tctggcgatt cgggatgccc tgatgcgtgc accagagccc gttaaccaat attgcgctgt
     7141 tacttttaca atgcccggaa tgtaaaccgt atattcgtcg tctggtgatc atgggcggtt
     7201 ctgctggtcg cggcaactgt acaccaaatg ccgagtttaa tatcgctgcc gatccagaag
     7261 cggctgcctg tgtcttccgt agcggcatcg aaattgtcat gtgcggtctg gatgtcacca
     7321 atcaggcaat attaacccct gactatctcg ctactttacc ggagttgaat cgcaccggaa
     7381 aaatgctcca cgccctgttt agccactacc gtagcggcag tatgcaaagc ggtctgcgaa
     7441 tgcacgatct ctgcgccatc gcctggctgg tgcgcccgga cttattcacc ctcaagccgt
     7501 gttttgtcgc ggtagaaact caaggcgaat ttacctcagg cacgacggtg gttgatatcg
     7561 acggttgcct gggcaaacca gccaatgtac aggtggcatt ggatctgaat gtaaaaggct
     7621 tccaacagtg ggtggctgag gtgctggctc tggtgccgta acctgtcaca tgttatcggc
     7681 atgcagtcat tcatcgactc atgcctttca ctgatatccc tccctgttta tcattaattt
     7741 ctaattatca gcgtttttgg ctggcggcgt agcgatgcgc tggttactct gaaaacggtc
     7801 tatgcaaatt aacaaaagag aatagctatg catgatgcaa acatccgcgt tgccatcgcg
     7861 ggagccgggg ggcgtatggg ccgccagttg attcaggcgg cactggcatt agaaggcgtg
     7921 cagctgggcg cggcgctgga gcgcgaagga tcttctttac tgggcagcga cgccggtgag
     7981 ctggctggtg cagggaaaac aggcgttacc gtgcaaagca gtcttgatgc gataaaagat
     8041 gattttgatg tgtttatcga ttttacccgt ccggaaggta cgctgaacca tctcgctttt
     8101 tgtcggcagc atggcaaagg gatggtgatc ggcactacgg ggtttgacga agccggtaaa
     8161 caggcaattc gtgacgccgc cgccgatatt gcgattgtct ttgccgccaa ttttagcgtt
     8221 ggcgttaacg tcatgcttaa gctgctggag aaagcagcca aagtgatggg tgactacacc
     8281 gatatcgaaa ttattgaagc acatcataga cataaagttg atgcgccgtc aggcaccgca
     8341 ctggcaatgg gagaggcgat cgcccacgcc cttgataaag atctgaaaga ttgcgcggtc
     8401 tacagtcgtg aaggccatac cggtgaacgt gtgccaggta ccattggttt tgctaccgtg
     8461 cgtgcaggtg acatcgttgg cgaacatacc gcgatgtttg ccgatattgg cgagcgtctg
     8521 gagatcaccc ataaggcgtc cagccgcatg acatttgcta acggtgcggt aagatcggct
     8581 ttgtggttga gtggtaagga aagtggtctt tttgatatgc gagatgtgct tgatctcaat
     8641 aatttgtaac cacaaaatgt ttattatgat gcaaaagtaa cacatttaat tta
//
LOCUS       DAZXUL010000158         8679 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZXUL010000158 DAZXUL010000000
VERSION     DAZXUL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.158"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(42..134)
                     /locus_tag="ACH3L1_003780"
                     /pseudo
     CDS             complement(42..134)
                     /locus_tag="ACH3L1_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(813..2009)
                     /gene="hemY"
                     /locus_tag="ACH3L1_003781"
     CDS             complement(813..2009)
                     /gene="hemY"
                     /locus_tag="ACH3L1_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFT4078338.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2012..3217)
                     /gene="hemX"
                     /locus_tag="ACH3L1_003782"
     CDS             complement(2012..3217)
                     /gene="hemX"
                     /locus_tag="ACH3L1_003782"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFT4078339.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3239..3979)
                     /gene="hemD"
                     /locus_tag="ACH3L1_003783"
     CDS             complement(3239..3979)
                     /gene="hemD"
                     /locus_tag="ACH3L1_003783"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFT4078340.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3976..4917)
                     /gene="hemC"
                     /locus_tag="ACH3L1_003784"
     CDS             complement(3976..4917)
                     /gene="hemC"
                     /locus_tag="ACH3L1_003784"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFT4078341.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5304..7850
                     /gene="cyaA"
                     /locus_tag="ACH3L1_003785"
     CDS             5304..7850
                     /gene="cyaA"
                     /locus_tag="ACH3L1_003785"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFT4078342.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7890..8210)
                     /gene="cyaY"
                     /locus_tag="ACH3L1_003786"
     CDS             complement(7890..8210)
                     /gene="cyaY"
                     /locus_tag="ACH3L1_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFT4078343.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8258..>8679)
                     /locus_tag="ACH3L1_003787"
     CDS             complement(8258..>8679)
                     /locus_tag="ACH3L1_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFT4078344.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1976 a         2240 c         2235 g         2228 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagcagttga cggggtatct
       61 gctgcgctgg ccatgagagg aagagcggcg gcgacagcaa caacaagacg tttgggtgaa
      121 aacgaaaatt ccatgcaaaa tgctccggtt tcatgtcatc aaaatgatga cgtaattaag
      181 cattgataat tgagatccct ctccctgaca ggatgattac ataaataata gtgacaaaaa
      241 taaattattt atttatccag aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac
      301 ctctggcgca gttgatatgt aaggcaggtt tattatatcg cgttgattat tgatgctgtt
      361 tttagtttta acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa
      421 taagtatgtg taggatcaag ctcaggttaa atatacactc aggaagttat tactcaggaa
      481 gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct
      541 ctgtggcata ataaacgagt agatgctcat tccatctctt atgttcgcct tagtgcctca
      601 taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct
      661 tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca
      721 gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgca tcagaaaggt
      781 cccggaggtg ctttcctccg ggtgagaagg aactactgtg gcgggttatt ctgcaacgtt
      841 aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc
      901 gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag
      961 gcgagcgatg cttcctgcca ttcaccgtgc ttcatcagtg actgacccaa tgtgctccac
     1021 aacagcgggc gatcaccgac gtttttgatt tgctggcgta gcactttttc cagctgttcc
     1081 gggttattgg ttttcagacg cgggatcggc agcagtaggc gatcgtcata ctggcgtttc
     1141 aggccgtcga tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc
     1201 gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag
     1261 ttacgcaaac cttcactacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc
     1321 tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg
     1381 atgatatcca gcagagaact ccatgcccct gtgcggatat acgcctgttc cgccagacgt
     1441 aatacttcag gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca
     1501 tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca
     1561 ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt
     1621 tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttcagc gtgatcggca
     1681 tttttcgcca tcagcttttc aacttgctga taatcacctt ccgccagttt cagcagcgcc
     1741 tgttcggtct gcttacgcgc acggcgacgc ttacgtccga caaaccatcc acgggtgtgc
     1801 gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc
     1861 agaatcaata tgatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg
     1921 atcagcacat aaccctgatg accggcaatc atcgggccaa ccactatccc cgcaatcagc
     1981 aaaacaaaga gtaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag
     2041 tatcaccttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt ttagcttccg
     2101 ttgtccccgc tgccggttgt gccagcaggt tacgcacgcg agtctgcatc agtttttcca
     2161 gcatcgcctg gctttgcagg gtttccggaa gatccatcga gatattttgc tggcttaact
     2221 ggtccacctc gtcgaggaac gctttggtgg tggcatcatc agtatcgtag taagcacgta
     2281 cccaggtgga gacgttctcc agcgcctggc gataagtctc ttcctgatga cgcggtacag
     2341 cttgtgctgc gaccagcagg cgagagcgaa tattttcgcg cagatagata tcctgatttg
     2401 gcgctaacag cggtacggcg gtgtcatcac gacggcgaat cgtaatgaag ttgtccataa
     2461 agttctgcca gcttttttgc agattgatac gccattcgct gatggaactg gaaagctctt
     2521 caccgtctga atccatcggc gaaccatcgc tgtcattatc ggccagacgc aggttatcta
     2581 cctgatttga aagctgatta agcttaagga tgatgccgtc ataatccacc tgcgatactg
     2641 cagaaaggct ggcgatatca tcggtaattg cccgacgaac ggtaatcaga ctcgggtcat
     2701 tcatatccgc caggctggcg tctgcacttt tcagcaacgc tgcagaggtc gtgacgtcct
     2761 gatcgctcca cagcttccgt ccggcgagtt tcaccagaaa atcggcctga gccagcagcc
     2821 aggttttagc atcgctgccg gaaatggtgg cgatcttttg ttggacttca tccaactgtt
     2881 ttgccagcgt ttcttgctga cgattcgcct gcttaagctg cgcagcttgt tgcttaataa
     2941 tgccttccag ctcggctttt tggctctcct gggctttttg caatgccgtc agttggttag
     3001 ccagggcatc gctggtggcg gtctgattga cggcctgttg tttaccccag ccatacaaac
     3061 cgacacccgc cgccagagca atagcgatag ccaccgcgct gagaatcaat gcagtattgt
     3121 tcttactctt tttttctgtt gcgacaggtt gtgacgtggt gtccacggcc tccctggtct
     3181 cttcaaccac ggcggaggtt ttttcttgtt ccgtcattat ggcttcctgt tatgagagtt
     3241 attgtaatgc ccgtaaaagc gcatcgttgt cagcgttatc ggcgacctta atgtcttgcc
     3301 agcccagttc ccgggcgagt ttcgccaaac gctcactgac gaccaatagt cgacagtgta
     3361 gtaaccagtg ctcacgatac cattgtggga tcagcgacca gagttgctgc aacatttcac
     3421 cgctggtaac aacgaccatc gtcacctcgc gggattgcca gcgcatcgct tcttctgcac
     3481 catcgtaatg gattgcgcat cgttgataac attcacaaaa agtgacctca gcaccgcgca
     3541 ccgtcagggt atccccaatt agctcacgac cgccattgcc acgtaatatc agcgcacgtt
     3601 tgcccgcaat attttgtaat tcaggtaatt gtagcaagac ttcgctgatt tcccgatcct
     3661 gtgggtagag aatcttctgt ccgcttacgg tatgtagcgc cagtgcggtg gtgcgtccaa
     3721 tggcgaaata agtaggtagt tggggccatt tacgatcttg ctgatgcagc tgtgattggg
     3781 caaaagcaac cgcgtgttgc gagagggcaa acaacagatc gctctccccc agcgctgcca
     3841 gttgatcagc aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc ggaaaatgcc
     3901 aggccacctg ccccagtgtg cgcagacggc tcactaactc ttctccagcg ggagacgggc
     3961 gggtgaccag gatactcatg ccggggcctc tccgttatag acttcagcga ggatctcgcg
     4021 cgcgccgtta ttcagtagct cttctgccag cgaaatcccc atttgttcgg catcttgcgg
     4081 cgcaccgcgg cgttcaccgc gaataatctg cgaaccgtcc ggcgcgccga ccagcgcaca
     4141 tagccagatt tcgccatcaa taagctcggc gtagctacca attggcacct gacatccgcc
     4201 ttcgagacgg gtattcatgg cgcgttctgc ggtaacgcgc agtgcagttt cgtggtgatt
     4261 cagcgcggca agcagctcgc gagtgcgtgt atcatcaagg cggcattcaa taccgactgc
     4321 accttgtcct accgccggaa gagaaatctc gggtggtaac gcggcgcgaa tgcgggactc
     4381 cagacctaaa cgttttagtc cggctacggc aagaatgatg gcatcgtatt cgccgttatc
     4441 tagtttgctc aggcgagtgc cgacgttgcc gcgcagggag cggataatca gatccgggcg
     4501 gcgttcagcc agttggcact ggcgacgtaa actggacgtc ccgacgatac tgcctgctgg
     4561 taacgcatcc agactgtcat agttattgga cacaaaggca tcgcgaggat cttcacgctc
     4621 gcaaatagtg accagcccca gaccttgcgg gaattcaacc ggcacatctt tcattgaatg
     4681 tacggcgata tcagcgcgat tctccaggag cgcgacttcc agctctttta caaataagcc
     4741 ttttccgcct actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg tcaccatcgg
     4801 taccagttca acgaccaggc ccggatggct cgccatcaac ttgtctttga catagtgtgc
     4861 ctgccagagt gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt ctaacatgct
     4921 tgttaccgtc attatcatcc gtggtccatc ctaacatcct tgccagagtg atgtcagtgt
     4981 tgtggtgaaa cgtagacgcc tgcgcaaacc gtaaaatgag gtctggcagt ggatcctgac
     5041 aggcgtttca cgccgtcgta ataaggaatt tacagagaat aaacggtgct acacttgtat
     5101 gtagcgcatc tttctttacg gtcaatcagc aaggtgttaa attgatcacg ttttagacca
     5161 ttttttcgtc gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt acctttgaca
     5221 tacgaaatat cccgaatgcc gcgtgttacc gttgatgttg gcggaatcac agtcatgacg
     5281 ggtagcaaat caggcgatac gtcttgtacc tctatattga gactctgaaa cagagactgg
     5341 atgccataaa tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct gcattccaac
     5401 aggtctacag tctactgccg acattgttgc actatcacca tccgcgaatg ccgggttacc
     5461 ttgatggtaa cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact caacgccact
     5521 acctgaacga gcttgaactg tatcgtggaa tgtcagtaca ggatccaccg aaaggtgagc
     5581 ttccaattac tggtgtatac accatgggca gcacctcgtc cgtagggcaa agttgttcct
     5641 ctgacctgga tatctgggtc tgtcatcaat cctggctcga tagcgaagag cgccagttac
     5701 tacaacgtaa atgcagcctg ctggaaagct gggccgcctc gctgggtgtg gaagtcagct
     5761 tcttcctgat tgatgaaaac cgcttccgtc ataatgaaag cggcagcctg gggggcgaag
     5821 attgtggctc cacccagcat atactgctgc ttgacgaatt ttatcgtact gccgtgcgtc
     5881 tcgccggtaa gcgtattctg tggaatatgg tgccgtgcga cgaagaagag cattacgacg
     5941 actatgtgat gacgctttac gcgcagggcg tgctgacgcc aaatgaatgg ctggatctcg
     6001 gtggcttaag ctcgctttct gctgaagagt actttggtgc cagcctttgg cagctctaca
     6061 agagtatcga ttccccatac aaagcggtgc tgaaaacact gctgctggaa gcctattcct
     6121 gggaataccc gaacccacgt ctgctggcga aagatatcaa acagcgtttg cacgacggcg
     6181 agattgtatc gtttggcctc gatccatact gcatgatgct ggagcgtgtt actgaatacc
     6241 tgacggcgat tgaagatttc acccgtctgg atttagtacg tcgctgcttc tatttaaaag
     6301 tgtgcgaaaa actcagccgt gaacgcgcct gtgtaggctg gcgtcgcgca gtgttgagcc
     6361 agttagtgag cgagtggggt tgggacgaag ctcgtctggc aatgctcgat aaccgtgcta
     6421 actggaagat tgatcaggtg cgtgaggcgc acaacgagct gctcgacgcg atgatgcaga
     6481 gctaccgtaa tctgatccgc tttgcgcgtc gcaataacct tagcgtctcc gccagtccgc
     6541 aggatatcgg cgtactgacg cgtaaactgt atgccgcgtt tgaagcattg ccaggtaaag
     6601 tgacgctggt aaacccgcag atttcacccg atctctcgga accgaatctg acctttattt
     6661 atgtgccgcc gggccgggct aaccgttcag gttggtatct gtataaccgc gcgccaaata
     6721 ttgagtcgat catcagccat cagccgctgg aatataaccg ttacctgaat aaactggtgg
     6781 cgtgggcatg gtttaacggc ctgctgacct cgcgcacccg tttgtatatt aaaggtaacg
     6841 gcattgtcga tttgcctaag ttgcaggaga tggtcgccga cgtgtcgcac catttcccgc
     6901 tgcgcttacc tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatt cgccatctgg
     6961 cgattatcgt taacctggaa tatgacccga cagctgcgtt ccgcaatcag gtggtgcatt
     7021 ttgatttccg taagctggat gtcttcagct ttggcgagaa tcaaaattgc ctggtaggta
     7081 gcgttgacct gctgtaccgc aactcgtgga acgaagtgcg tacgctgcac ttcaacggcg
     7141 agcaatcgat gatcgaagcc ctgaaaacta ttctcggcaa aatgcatcag gacgccgcac
     7201 cgccagatag cgtggaagtc ttctgttata gccagcatct gcgcggctta atccgtactc
     7261 gcgtgcagca actggtttct gagtgtattg aactgcgcct ttccagcacc cgccaggaaa
     7321 ccgggcgttt caaggcgctg cgcgtttctg gtcaaacctg gggcttgttc ttcgaacgcc
     7381 tgaatgtatc ggtacagaag ctggagaacg ccatcgagtt ttatggcgct atttcgcata
     7441 acaaactgca cggcttgtca gtgcaggttg aaaccaatca cgtcaaatta ccggcggtgg
     7501 tggacggctt tgccagcgaa gggatcatcc agttcttttt cgaagaaacg caagacgaga
     7561 atggctttaa tatctacatt ctcgacgaaa gcaaccgggt tgaggtgtat caccactgcg
     7621 aaggcagcaa agaggagctg gtacgtgacg tcagtcgctt ctactcgtca tcgcacgacc
     7681 gcttcaccta cggctcaagc ttcatcaact tcaacctgcc gcagttctat cagattgtga
     7741 aggttgatgg tcgcgaacag gtgattccgt tccgaacaaa atcgattggc aacctgccgc
     7801 ccgccaatca ggatcacgat acgccgctat tacagcagta tttttcgtga tgagcgtgcc
     7861 ggaaagcgag gcttatccgg catgcaatct tagcggaaac tgactgtttc acccgcctgt
     7921 tgcgtcgccg cctgttccag caaatcccag aaggtttcgc cgctgcgatc gcaaatccat
     7981 tcatcgcctt tcaggtcaaa atggtagccg ccctgtttgg ttgccagcca cacctggtgc
     8041 agcggctcct ggcggttaat aatgattttg ctgccgtttt caaaggtaat ggtcagtacg
     8101 ccgccgttga tttcgcagtc gatatcgctg tcgccatccc tgtcgtccag acgctcttca
     8161 atggtcagcc acagttgatc agccaggcga tgaaattcac tgtcgttcat tgttgtatcc
     8221 tgtttttaag tgatggcggc agtatagcgg catggggtca gggcttcaaa gtttgcacct
     8281 ctgctgctgc gttccggcat gattcatccg tcaccggaat aatgatgtct ctgttttgaa
     8341 cttcacgtac gttctcctct ctgacacgag ttttcgccgg gcagggggag ggcgtaatcc
     8401 ttacctctgt atcggtccat gtataaacat tgcgatcgtc ataagcgaag cgatcgtttt
     8461 tagttagcga accgttaact ggaataattt gcaagctctt tgaccgtaat tggcggataa
     8521 agtaagcatg ctgtttgccg ctattaaatg agcgatcaat gaccaggtct ggcgtatcca
     8581 gcgggattat ctcaagctgg tcttcaccaa atgtcgataa ttttgttaca tacagccgat
     8641 ctttatattg cgcaatattg ccagtaatca gatggaatt
//
LOCUS       DAZXUL010000159         8548 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZXUL010000159 DAZXUL010000000
VERSION     DAZXUL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.159"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            16..270
                     /gene="yjbL"
                     /locus_tag="ACH3L1_003788"
     CDS             16..270
                     /gene="yjbL"
                     /locus_tag="ACH3L1_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFT4078345.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            294..1001
                     /locus_tag="ACH3L1_003789"
     CDS             294..1001
                     /locus_tag="ACH3L1_003789"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFT4078346.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            1364..2401
                     /gene="dusA"
                     /locus_tag="ACH3L1_003790"
     CDS             1364..2401
                     /gene="dusA"
                     /locus_tag="ACH3L1_003790"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFT4078347.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2535..2777
                     /gene="pspG"
                     /locus_tag="ACH3L1_003791"
     CDS             2535..2777
                     /gene="pspG"
                     /locus_tag="ACH3L1_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFT4078348.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(2943..3926)
                     /locus_tag="ACH3L1_003792"
     CDS             complement(2943..3926)
                     /locus_tag="ACH3L1_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFT4078349.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            4009..5424
                     /gene="dnaB"
                     /locus_tag="ACH3L1_003793"
     CDS             4009..5424
                     /gene="dnaB"
                     /locus_tag="ACH3L1_003793"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFT4078350.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5477..6556
                     /gene="alr"
                     /locus_tag="ACH3L1_003794"
     CDS             5477..6556
                     /gene="alr"
                     /locus_tag="ACH3L1_003794"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFT4078351.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6809..8002
                     /gene="tyrB"
                     /locus_tag="ACH3L1_003795"
     CDS             6809..8002
                     /gene="tyrB"
                     /locus_tag="ACH3L1_003795"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFT4078352.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(8225..8548)
                     /locus_tag="ACH3L1_003796"
                     /pseudo
     CDS             complement(8225..8548)
                     /locus_tag="ACH3L1_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027387.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2166 a         1963 c         2151 g         2268 t
ORIGIN      
        1 aaaggtagat gaatcatgtt gaagataata cccggtgcaa ctggatactt taataaaaca
       61 ctcaattcaa atcaattcga aaataaagat gcaaccaaag ataaattgga taatagaggt
      121 tcaatcaaag gtaaattaaa taatatatat ggtaaatcta ttgattatgc agcgctgcgt
      181 catcgggata taattattgc aaaaatagat ttgtttattc aacgaatcac gcataattta
      241 tggcatgcac gtaaaaaaat gtgtttttaa ttgagcacat aaatgatctc aaaatgtggg
      301 tcaataagta tactgacgat tgcactgatg aggatttaaa cgatcgtgac tttattgcat
      361 cagttgttga ccgggctatt tttcatttcg cgattaatag tatatgtaat cctggggata
      421 ataaagatgc aatgcccatt gaacaatgta cttttgatgt agaaactaag aatggccttc
      481 cctccacggt tcagctattt tatgaggaat ctaaggataa tgaaccttta gcgaatatac
      541 attttcaagc aataggttct ggttttttaa cgtttgttaa tgcctgccag gaacatgatg
      601 acaacagctt aaaattattt gcttcgctgt taatttcact ttcatattct agtgcctacg
      661 cagatttatc agaaacagtg tatattaatg aaaataatga gagctacctg aaagctcagt
      721 ttgaaaaatt atatcaacgt gatatgaaga agtacctggg agagatgaag cgtctggcag
      781 atgggggaga aatgaatttt gatggctatc tggataagat gtcacatctg gtgaatgaag
      841 gaacgctcga tcctgatatt ttaagcaaaa tgcgagatgc tgcaccacaa ttaattagct
      901 tcgcgaagtc gtttgaccca acctcaaagg aagagattaa aatacttaca gacacttcta
      961 aattaattta tgacttgttc ggggttaaat cggagaaata atatgtgaag ttcttcgata
     1021 gtatggaagg cattatataa aaggacccaa tatttattgg gttctttttt ctctatcaat
     1081 gctattagca gggagatata tcaccagagt ttaatgtgtg attttttatt tatcgtcgaa
     1141 cctggattgt ttatcattgg ccttaacaaa gttaacggct aataagatta tttccatcac
     1201 ttcgtgagag cttcatgcct tgagaggatc tcaattttct tttgcaatga gacaggcgct
     1261 tcctgttgtt atggtatagt accccgctat tgagcctcct gaacagtgaa gctgaataac
     1321 ataaccccat gatatatcga taaaataatc tctacatttg aaaatgcacg gtaattctga
     1381 aatgcaaaaa atcaaccaaa ccagcgcaat gcctgaaaaa actgacgttc actggagtgg
     1441 tcggtttagt gttgcaccaa tgctcgactg gacggacaga cattgccgct atttcttgcg
     1501 tctgctttcc cgcaatacgt tgctttatac cgaaatggtg accacagggg cgataattca
     1561 cggtaaaggt gattatctgg cgtacagtga agaagaacat ccggtagcgt tgcaactggg
     1621 cggtagcgat ccggcggcgc tggcgcagtg tgcaaagctg gcagaagcgc gcggatatga
     1681 tgagatcaac ctgaatgtcg gctgcccgtc tgaccgggtg cagaacggca tgtttggtgc
     1741 gtgtctgatg ggtaatgcgc agctggttgc cgactgcgtg aaagcgatgc gcgatgtggt
     1801 gtcgattccg gtgacggtga aaacgcgtat tggcatcgac gaccaggaca gctatgaatt
     1861 tctctgcgat ttcatcaata ccgtttccgg caaaggcgag tgtgagatgt tcatcatcca
     1921 cgcacgtaaa gcctggcttt cggggttaag cccgaaagaa aaccgtgaaa tcccgccgct
     1981 cgattatccg cgtgtgtatc aactgaagcg tgacttcccg catctgacaa tgtcgattaa
     2041 cggtggtatc aagtcgctgg aagaagctaa agcgcatttg caacatatgg atggcgtgat
     2101 ggtcgggcgc gaggcgtatc agaatccggg tattctggcg gcggtagacc gggagatctt
     2161 tggttcctcg gataccgatg ccgatccggt ggcagtagtg cgtgccatgt atccgtacat
     2221 tgagcatgaa ctcagccagg ggacgtatct cggccatatt atccggcata tgctgggttt
     2281 gttccagggt attcctggcg cgcggcagtg gcggcgttat ttaagtgaaa atgcccataa
     2341 agcgggtgct gacattaacg tgctggaaca cgcgctcaaa ctggtggcgg ataagcgtta
     2401 acttttcacc aaaaagtagt caaattcacc acgccctgcg caccgtcgcg gggcgttttg
     2461 ctgttaaatc aatagattat ttttggcatg attcttgtaa tgccagcaag agatttcata
     2521 tttgggagag catcatgctg gaactacttt ttgtgattgg cttttttgtc atgctgatgg
     2581 tcaccggcgt ttcgttgctg ggcattatcg ccgcgctggt tgtggcgacg gccattatgt
     2641 tcctcggcgg tatgctggca ttgatgatta agttgctgcc gtggttacta ctggcgattg
     2701 cggtggtgtg ggttattaag gcgattaaag caccaaaaat gccgaaatat cagcgttatg
     2761 accgctggcg ttactaaggg attgtgcgga tgatcacaac ctaaggtttt atccttagaa
     2821 caaaatagga attgataatc aaatctgtca ctattgcgcc tctaacagat tcatcgtgct
     2881 gtaccctaca tacagccgaa ctataaaaag aaagggcttc ccaggtggaa gccctatttc
     2941 ttttatggaa tcagcaggct ggaaccttgc gtcgcccggc tttccagaat ctcatgcgca
     3001 cgctgcgcat ccttcagcgg atatttctgc tgctcggcga catcgacctt aatcacaccg
     3061 ctggcaatca aagagaacag ttcattactg gcctcggtta attcctcccg cgtggtgata
     3121 tagccttgca gggaagggcg tgtcacatac aacgagcctt tttgattgag aatgcctaag
     3181 ttcacaccgg taaccgcacc tgatgagttg ccaaaactga ccattaagcc gcggcgttgc
     3241 aggcaatcca gcgaccgttc ccaggtgtct ctgcccacgg aatcgtacac tacgcgcact
     3301 ttcttaccgc cggtgatctc tttcaatcgc tcgaccagat tctcttcacg atagttaata
     3361 acctgccacg cgccggcttt tagcgcgctc tgcgcttttt gcgcggttcc tacggtgccg
     3421 atgagtttcg cgcccagggc ttttgcccac tggcaggcaa ttaagccaat gccgccagcc
     3481 gctgcgtgga acaggaactg ttcatcgggt ttaatttcat aggttttgcg cagcagataa
     3541 taaaccgtta agcctttcag gaaggatgcc gcagcttgct caaaagaaat tgccgcaggc
     3601 agaatcgccg ctttatccgc attaatgtta tgcacagagc tgtaagcgcc taacgccgac
     3661 tgcgcataga ctacatgatc gccagcctta atatgcttta caccactgcc gactttactc
     3721 acgatgcctg ccgcttcggt acctaatccg ctgggtagcg atggcggcgg gtaaaggccg
     3781 ctgcggatat acgtgtcgat aaaattgatg ccgatggctt tattttcgac ctggatttca
     3841 ttctccgccg gatcggcagg agtgaactct acggcttgaa gtacttccgg gccaccgtgc
     3901 ttgtgaaatt caattcgtgt tgccatgtgt cctccagaac gtatcgtcag ggtctgcttc
     3961 atatggtaaa gtttcgaccc attctttatt tcggtaactc cattcactat ggcaggaaat
     4021 aaacccttca acaaacagca ggctgaaccc cgcgaacgcg atccacaagt tgccgggctg
     4081 aaagtgcctc cgcactcgat cgaagcggag cagtcggtgt tgggcggttt aatgctggat
     4141 aacgaacgct gggatgatgt agccgagcgt gtggtagcag acgattttta cacccgccca
     4201 caccgtcata tctttactga aatggcgcgt ttgcaggaaa gcggtagtcc tatcgatctg
     4261 attacccttg cggaatcgtt ggaacgccag gggcaactcg atagcgtcgg tggttttgct
     4321 tatctggcag agctgtcaaa aaatacgcca agtgcggcga acatcagtgc ttatgcggac
     4381 atcgtgcgtg aacgtgccgt tgtccgtgag atgatctcgg ttgcgaatga gattgctgaa
     4441 gccggttttg atccgcaggg gcgtaccagc gaagatctgc tggaccttgc tgaatcccgc
     4501 gtctttaaaa ttgccgaaag tcgtgcaaac aaagacgaag ggccgaagaa catcgccgat
     4561 gtgctcgacg caaccgtggc gcgtattgag cagttgtttc agcagccaca cgatggcgtt
     4621 accggggtaa acaccggtta tgacgatctc aacaaaaaaa ccgctggctt gcagccgtcg
     4681 gatttgatca tcgtcgccgc gcgtccgtcg atgggtaaaa caacatttgc gatgaacctc
     4741 gtcgaaaacg cggcaatgtt gcaggataaa ccagtactta tcttctcgct ggagatgcca
     4801 tcagaacaga tcatgatgcg ttctctggcg tcgctgtcgc gcgttgacca gactaaaatc
     4861 cgtaccgggc agctcgatga tgaagactgg gcgcgcattt ccggcaccat gggtattttg
     4921 ctcgaaaaac gcaatatcta tatcgatgac tcttccggcc tgacgccaac ggaagtgcgt
     4981 tcccgcgcac gccgtattgc ccgtgaacac ggcggcatcg ggcttatcat gatcgactac
     5041 ctgcaactga tgcgcgtacc ggcgctttcc gataaccgta cgctggaaat tgcagaaatc
     5101 tcccgctcgc tgaaagcact ggcgaaagaa ctgaacgtgc cggtggtggc gctgtcccag
     5161 ttgaaccgtt ctctggaaca acgtgccgac aaacgtccgg tcaactccga cctgcgtgaa
     5221 tctggctcta tcgagcagga tgcggacttg atcatgttta tctatcgtga tgaggtgtat
     5281 cacgaaaaca gtgatttaaa aggcatcgcg gaaattatta tcggtaaaca acgtaacggc
     5341 ccaatcggga cggtacgcct gacctttaac ggtcaatggt cgcgcttcga caactatgcg
     5401 gggccgcagt acgacgacga ataataatta ttttatgaat taggtaatta aagcaaacaa
     5461 ttatcaagga acacaaatgc aagcggcaac tgttgtgatt aaccgccgcg ctctgcgaca
     5521 caacctgcaa cgtcttcgtg aactggcacc tgccagtaaa atggttgcgg tggtgaaagc
     5581 gaacgcttat ggtcacggtc ttcttgagac cgcgcgaacg ctccccgatg ctgacgcctt
     5641 tggcgtagcc cgtctcgaag aagctctgcg actgcgtgcg gggggaatca ccaaacctgt
     5701 actgttactc gaaggctttt ttgatgccag agatctgccg acgatttctg cgcaacattt
     5761 tcataccgcc gtgcataacg aagaacagct ggctgcgctg gaagaggcta gcttggacga
     5821 gccggttacc gtctggatga aactcgatac cggtatgcac cgtctgggcg taaggccgga
     5881 acaggctgag gcgttttatc atcgcctgac ccagtgcaaa aacgttcgtc agccggtgaa
     5941 tatcgtcagc cattttgcgc gcgcggatga accaaaatgt ggcgcaaccg agaaacaact
     6001 cgctatcttt aatacctttt gcgaaggcaa acctggtcaa cgttccattg ccgcgtcggg
     6061 tggcattctg ctgtggccac agtcgcattt tgactgggtg cgcccgggca tcattcttta
     6121 tggcgtctcg ccgctggaag atcgctccac cggtgccgat tttggctgtc agccagtgat
     6181 gtcactaacc tccagcctga ttgccgtgcg tgagcataaa gccggagagc ctgttggtta
     6241 tggtggaacc tgggtaagcg aacgtgatac ccgtcttggc gtagtcgcga tgggctatgg
     6301 cgatggttat ccgcgcgccg cgccgtccgg tacgccagtg ctggtgaacg gtcgcgaagt
     6361 accgattgtc gggcgcgtgg cgatggatat gatctgcgta gacttaggtc cacaggcgca
     6421 ggacaaagcc ggggatccgg tcattttatg gggcgaaggt ttgcccgtag aacgtatcgc
     6481 tgaaatgacg aaagtaagcg cttacgaact tattacgcgc ctgacttcaa gggtcgcgat
     6541 gaaatacgtg gattaatcgt tctgtaatat ttgattgtct gtgccggatg cggcgtgaat
     6601 gccttatccg gccaataaaa tcctaaaaat tcaataagtt gatgttcttt catgctctta
     6661 taaaggtcgt gcctctggcg gatgtacgtt tatcattagt ctcactctgt tgctaattgc
     6721 cgttcgctcc tgaacatcca ctcgatcttc gccttcttcc ggtttattgt gttttaacca
     6781 cctgcccgta aacctggaga accatcgcgt gtttcaaaaa gttgacgcct acgctggcga
     6841 cccgattctt acgcttatgg agcgttttaa agaagaccct cgcagcgaca aagtgaattt
     6901 aagtatcggt ctgtactaca acgaagacgg aattattcca caattgaaag ccgtggcgga
     6961 ggcggaagcg cgcctgaatg cgcagcccca tggcgcttcg ctttatttac cgatggaagg
     7021 gcttaacagc tatcgccatg ccattgcgcc gctgctgttt ggtgcggacc atccggtact
     7081 gcaacaacag cgcgtagcaa ccattcaaac ccttggcggc tcaggggcat tgaaagtggg
     7141 cgcggatttc ctgaaacgct acttcccgga atcaggcgtc tgggtcagcg atcctacttg
     7201 ggaaaaccac gtagcaatat tcgccggggc tggattcgaa gtgagtactt acccctggta
     7261 tgacgaagtg actaacagcg tgcgctttaa tgacctgttg gcgacgctga aaacattacc
     7321 tgcccgcagt attgtgttgc tgcatccatg ttgccacaac ccaacgggtg ccgatctcac
     7381 taatgatcag tgggatgcgg tgattgaaat tctcaaagcc cgcgagctta tcccattcct
     7441 cgatattgcc tatcaaggat ttagtgccgg tatggaagag gatgcctacg ctattcgcgc
     7501 cattgccagt gctggattac ccgctctggt gagcaattcg ttctcgaaaa ttttctccct
     7561 ttacggcgag cgcgtcggcg gactttctgt tctgtgtgaa gatgccgaag ccgctggccg
     7621 cgtactgggg caattgaaag caacagttcg ccgcaactac tccagcccgc cgaattttgg
     7681 tgcgcaggtg gtggctgcag tgctgaatga cgaggcattg aaagccagct ggctggcgga
     7741 agtagaagag atgcgtactc gcattctggc aatgcgtcag gaattggtga aggtattaag
     7801 cacagagatg ccagaacgca atttcgatta tctgcttaat cagcgcggca tgttcagtta
     7861 taccggttta agtgccgctc aggttgaccg actacgtgaa gaatttggtg tctatctcat
     7921 cgccagcggt cgcatgtgtg tcgccgggtt aaatacggca aatgtacaac gtgtggcaaa
     7981 ggcgtttgct gcggtgatgt aatgcaggaa agcaggctgg agttacccag cctgcagtga
     8041 aattaaactg tcgtcgcttt cactatttct ttgtagatga tttttttgat gccgttgttc
     8101 tacgtgagag ataataaaac gttgtcagtt cttttattgt taagtttatc ccaattatct
     8161 ggaattcctt tccctatttt ttcgtgtgga gtaataattt ctgcacatct aaccattatt
     8221 aaatttaata ataagtatcg ggtttgttac gatagaatat ttctatatcc tcaagaatat
     8281 gctttgcctt attgcctgtg actttctgag attcaattat agtatcgtta aattctaatg
     8341 ttaaagagaa ctcttttttc cgctttgtgt aagtttaacc ttatattgat taccaatatc
     8401 aaataaaaag gtcctgtaat gagaataatc acctttaacc tcctcggcaa aacagcaaag
     8461 cgtgcaaata tacataaagt ctttcttacc cctgattttg ttaactaaat caataaatgc
     8521 gtgaaaaact ttacttgcaa tagaactt
//
LOCUS       DAZXUL010000160         8528 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZXUL010000160 DAZXUL010000000
VERSION     DAZXUL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.160"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..189
                     /gene="csgC"
                     /locus_tag="ACH3L1_003797"
     CDS             <1..189
                     /gene="csgC"
                     /locus_tag="ACH3L1_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFT4078353.1"
                     /translation="GSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVS
                     DGQALHLSQQWTPSSEKS"
     gene            310..621
                     /gene="ymdA"
                     /locus_tag="ACH3L1_003798"
     CDS             310..621
                     /gene="ymdA"
                     /locus_tag="ACH3L1_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFT4078354.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            716..1249
                     /gene="ymdB"
                     /locus_tag="ACH3L1_003799"
     CDS             716..1249
                     /gene="ymdB"
                     /locus_tag="ACH3L1_003799"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFT4078355.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1191..2672
                     /gene="clsC"
                     /locus_tag="ACH3L1_003800"
     CDS             1191..2672
                     /gene="clsC"
                     /locus_tag="ACH3L1_003800"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFT4078356.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACH3L1_003801"
     CDS             complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACH3L1_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFT4078357.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4231..5766
                     /gene="mdoG"
                     /locus_tag="ACH3L1_003802"
     CDS             4231..5766
                     /gene="mdoG"
                     /locus_tag="ACH3L1_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFT4078358.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5759..8302
                     /gene="mdoH"
                     /locus_tag="ACH3L1_003803"
     CDS             5759..8302
                     /gene="mdoH"
                     /locus_tag="ACH3L1_003803"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFT4078359.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2150 a         2025 c         2222 g         2131 t
ORIGIN      
        1 ggcagttcag ggcaaagtca gacgaagcaa gaaaagaccc tctcattgcc tgctaatcaa
       61 cccattgctt tgacgaagtt gagtttaaat atttccccgg aagatcgggt gaaaatagtt
      121 gttactgttt ctgatggaca ggcacttcat ttatcacaac aatggacgcc ctcttcagaa
      181 aagtcttaat ttgttgaaat atcgagcata agatgaatct ggagagaatg gtctgctgcg
      241 aatcagccaa cctgaaagta tggataacac aaccttcaag gatgactaat cattgaggaa
      301 atagaataaa tgttcagacc ttttttagac tctcttatgc tcggcagttt gttttttcct
      361 tttattgcca ttgcaggaag caccgcgcaa gggggcgtga tccattttta tggccaaatt
      421 gtggaaccgg catgtgacgt cagcacccag tcatcacccg tagaaatgaa ctgcccacag
      481 aatggttcta ttccgggcaa aacctactcc agcaaagcgt taatgagcgg caatgtcaaa
      541 aacgcgcaaa tagcctcagt aaaagtgcag tatcttgata aacagaaaaa gctggctgta
      601 atgaacatcg aatataacta agtttctgaa caactcacgt tgctgagcag aaaaatgcga
      661 tttaaccaaa aagcctgctg tacacttaag aaacaagaag gtgtaaggag gcgttatgaa
      721 aacgcgtatt catgttgtgc agggtgatat taccaaactg gccgttgatg tgattgtgaa
      781 tgcggctaat ccgtcattaa tgggaggcgg cggcgtcgat ggggccattc atcgcgcagc
      841 gggtccggcc ctgctggatg cctgtttaaa agtcaggcaa cagcagggcg attgccctac
      901 ggggcatgcc gttattacgc ttgcaggcga tcttcccgct aaagccgtag tacacaccgt
      961 cgggccagtc tggcgtggtg gggaacaaaa cgaagaccag cttttgcagg atgcctatct
     1021 gaatagccta cgactggtgg cggcaaacag ctatacgtca gtggcttttc ctgcaatcag
     1081 tactggggtt tatagttacc ctcgtgcggc agcggctgaa atcgcagtta aaaccgtttc
     1141 agaatttatt acccgtcacg ctttacccga acaggtatac tttgtctgtt atgatgaaga
     1201 aaacgcccac ctctacgaaa gactccttac ccaacaagga gatgaatgat ttgccccggc
     1261 tggcgagcgc ggtgctgcca ctgtgttcgc aacatcccgg tcagtgtggt cttttccctc
     1321 tggagaaaag tctggatgcg tttgccgccc ggtatcgtct ggccgaaatg gcagagcata
     1381 cgctggatgt tcagtattac atctggcagg acgatatgtc ggggcggtta ctgttttccg
     1441 ccctgttagc cgcagcaaag cgtggcgttc gcgtccgttt gttgctggac gacaacaata
     1501 cgcccggact tgacgacatt ttgcgcttgc ttgacagtca tccacgcatt gaagtccggc
     1561 tttttaatcc tttctcgttt cgcttgctac gtccgcttgg atatatcacc gacttttccc
     1621 gtctcaatcg ccggatgcac aataaaagtt tcactgtcga tggcgtggtt accctagtgg
     1681 gaggacgaaa tattggtgat gcctattttg gtgcagggga agagccactt ttttcggatt
     1741 tagatgtcat ggcaataggc cccgtggtag aggatgttgc cgatgatttc gcccgctact
     1801 ggtattgcaa atcggtttca cccttacagc aggtgctgga tgtcccggaa ggtgaaatgg
     1861 cggatcgcat cgagttaccc gcctcctggc ataacgatgc catgacgcat cgttatttac
     1921 gcaaaatgga atccagtcca tttataaatc atctggttga tggaacgttg ccgcttatct
     1981 gggcgaagac acgtttatta agtgatgatc cggcgaaagg ggagggcaag gcaaaacggc
     2041 attcactgtt accgcagcgc ctgttcgata tcatgggctc acccagtgaa cgcatcgata
     2101 ttatctcttc ctattttgta ccgacacgcg caggtgtggc gcaactctta cggatggtga
     2161 gaaaaggggt aaagattgcg atcctaacca attctcttgc cgctaacgat gttgctgtcg
     2221 tccatgccgg atacgcgcgc tggcgcaaaa aattgctccg ctatggcgtg gaattatatg
     2281 aactcaagcc gacgcgtgaa caaagcagta cgttacacga tcgcggcata accggtaatt
     2341 ccggtgccag cctgcatgct aaaactttta gcatcgatgg taaaacggtg tttatcggct
     2401 ctttcaattt cgatccgcgt tcaacattgc tcaatactga aatgggcttc gtgatagaga
     2461 gcgaaacgct ggcacagtta attgataaac gctttattca gagccagtac gatgcggcct
     2521 ggcagctccg tctggacagg tggggacgga tcaactgggt tgatcgtcat gcaaagaaag
     2581 aggttattct caaaaaagaa cccgccacca gtttctggaa gcgggttatg gtcagactgg
     2641 cgtcgatatt gcccgtggaa tggttattgt aaatgtggct taacgggctg gtgctttatc
     2701 gttctcacgc ttaacaaccg gtttaccaga gaacaaaaac ttcagtaacg ggatgcgtaa
     2761 atgaatttca tacagaatta tcgcaatccc tactacgaat atcaggccac agagaaaacc
     2821 aagccagttg gaggtgatgt gcggtgtaat gtatgcgccg aaaaacagcg ttaacgggtg
     2881 gtgaaccaga tagataaaca gcgatgcgtt aacaaaataa gtcacccgcg ctgactggaa
     2941 gttaagcaaa cggtggccaa aggagaagac cacattcacc atccacaggc cgaggaccat
     3001 ggtgatcacc gactcggttt cgtacatcca ggcatcgcca ctgccatagc gctggttgag
     3061 taaataagcg acaaacgcca atgctgcagc gagggtacag ccacgagacg gcgtggtaaa
     3121 caaggcttta agatgaggga aaatgaaagc cagtgcgccg aggataaaga acggtaaata
     3181 aaatagcgtt tgcatgacaa taaaattgaa catgccatta ctcagaatgg gtggatacac
     3241 aataaaaatc gttcttctta ttaccgcata gccgatgccg aggcataaaa aaatcaccga
     3301 tagttttacc atcgagaatt ttttattcgt tttatcagaa ttttctaaat tatttctgat
     3361 gcgcttaaat atccatacac acagcgtcgt catgactact aacaccagta aaaaccacag
     3421 gtgtgatatt aattcccagg ccaacgtatt atatttgtca tacaatgata gcccaggcca
     3481 actttccgct ttccctttga catattgcag cataataaat tgcggtaatg tcagtagggg
     3541 gatggctgtt aacatcggga tacctacacg ttcgacacgt actttccacc attttttcaa
     3601 gggatagcgt aaaaaaagca tgtaggaaaa gtatccggat ataacgaaaa atacctgcat
     3661 gcggaacgag tggatgaagt cattaaaaag ggttagccac aatgacggtt cggcgctatt
     3721 cacatgccat gtatggctcg aatagattaa agaaatatga aaagggatcc ctaacaacat
     3781 cagccaggcg cggatggagt cgaggaaata ttcacgttgc gcgggtactg gattcatata
     3841 tggttaacta atctcggatt tttcgtctta tccctgtcgg gttatgcctt taggcttgtt
     3901 gccatagcga caccgacctg accgcgccag gcgcaggctt caaggttttt atgcatagca
     3961 tcatcgctac cactaaccag aatggaagcg tctgtaagac ggttgataaa taaatttgct
     4021 ggcaaaccct acacgaagtc gatgcttctg tctttaggag aagcacggaa agtgaaaacg
     4081 gttgcaatca ggtgcttaat ccatgagcca gcgtgctgaa cgataccggg attcagttgt
     4141 cggaatggct ggttatccat taaaatagat cggatcgata taagcacaca aagggggaag
     4201 tgcttactaa ttatgaaaca taaactacaa atgatgaaaa tgcgttggtt gagtgctgca
     4261 gtaatgttaa ccctgtatac atcttcaagc tgggctttca gtattgatga tgtcgcaaag
     4321 caagctcaat ccttagccgg gaaaggctat gaggcgccta aaagcaactt gccctccgtt
     4381 ttccgcgaca tgaaatacgc ggactatcag cagatccagt ttaatcatga caaagcgtac
     4441 tggaacaatc tgaagacccc attcaaactc gagttctacc atcagggtat gtacttcgat
     4501 accccggtca aaataaatga agtgactgcc accgcagtca aacgaatcaa atacagcccg
     4561 gattatttca ctttcggcga tgttcagcat gacaaagaca cggtaaaaga ccttggtttt
     4621 gccggtttta aagtgcttta cccgatcaac agcaaagata aaaacgatga aatcgtcagc
     4681 atgctcgggg ccagctattt ccgcgtgatt ggtgcaggtc aggtttatgg cctttctgcc
     4741 cgcggcctag caattgatac cgctttgcca tcgggtgaag aatttccacg cttcaaagag
     4801 ttctggatcg agcgtccaaa accgactgat aaacgtttaa ccatttatgc attgcttgac
     4861 tcgccgcgtg cgacaggtgc ttacaaattc gtggttatgc cagggcgtga cacggttgtg
     4921 gatgtgcagt cgaaaatcta tctgcgcgat aaagtcggca aactgggggt tgcaccgtta
     4981 accagtatgt tcctgtttgg gccgaaccaa ccgtcgcctg caaataacta tcgtccggag
     5041 ttgcacgact ctaacggtct gtctatccat gctggtaatg gcgaatggat ctggcgtccg
     5101 ttgaataacc cgaaacattt agcggtcagc agcttctcca tggaaaaccc gcgaggcttt
     5161 ggtctgttgc aacgaggtcg tgatttctcc cgctttgaag atctcgatga tcgttacgat
     5221 ctccgtccaa gcgcatgggt gactccgaaa ggggagtggg gcaaaggcag cgttgagctg
     5281 gtggaaattc caaccaacga tgaaaccaac gataacatcg tcgcttactg gacgccggat
     5341 cagctgccgg agccgggtaa agagatgaac tttaaataca ccatcacctt cagccgtgat
     5401 gaagacaaac tgcatgcgcc agataacgca tgggtgcaac aaacgcgtcg ttcaacgggg
     5461 gatgtgaagc agtcgaacct gattcgccag cctgacggta ctatcgcctt tgtggtcgat
     5521 tttaccggcg cagagatgaa aaaactgcca gaggataccc cggtcacagc gcaaaccagc
     5581 attggtgata atggtgagat agttgaaagc acggtgcgct ataacccggt taccaaaggc
     5641 tggcgtctgg tgatgcgtgt gaaagtgaaa gatgccaaga aaatcactga aatgcgtgct
     5701 gcgctggtga atgccgatca gacgttgagt gaaacctgga gctaccagtt acctgccaat
     5761 gaataagaca actgagtaca ttgacgcaat gcccatcgcc gcaagcgaga aagcagcatt
     5821 gccgaagact gatatccgcg ccgttcatca ggcgctggat gccgaacacc gcacctgggc
     5881 gcgggaggat gattccccgc aaggctcggt aaaggcgcgt ctggaacaag cctggccaga
     5941 ttcacttgct gatggacagt taattaaaga cgacgaaggg cgcgatcagc ttaaggcgat
     6001 gccagaagca aaacgctcct cgatgtttcc cgacccgtgg cgtaccaacc cggtaggccg
     6061 tttctgggat cgcctgcgtg gacgcgatgt cacgccgcgc tatctggctc gtttgaccaa
     6121 agaagagcag gagagcgagc aaaagtggcg taccgttggt accatccgcc gttacattct
     6181 gttgatcctg acgctcgcgc aaactgttgt cgcgacctgg tatatgaaga ccattcttcc
     6241 ttatcagggt tgggcgctga ttaatcctat ggatatggtt ggtcaggatt tgtgggtttc
     6301 ctttatgcag cttctgcctt atatgctgca aaccggtatc ctgatccttt ttgcggtact
     6361 gttctgttgg gtgtccgccg gattctggac ggcgttgatg ggcttcctgc aactgcttat
     6421 tggtcgcgat aaatacagta tatctgcgtc aacagttggc gatgaaccat taaacccgga
     6481 gcatcgcacg gcgttgatca tgcctatctg taacgaagac gtgaaccgtg tttttgctgg
     6541 cttgcgtgca acgtgggaat cagtaaaagc caccgggaat gccaaacact ttgatgtcta
     6601 cattctgagt gacagttata acccggatat ctgcgtcgca gagcaaaaag cctggatgga
     6661 gcttatcgct gaagtcggtg gcgaaggtca gattttctat cgccgccgcc gtcgccgcgt
     6721 gaagcgtaaa agcggtaata tcgatgactt ctgccgtcgc tggggcagcc agtacagcta
     6781 catggtggtg ctggatgctg actcggtaat gaccggtgat tgtttatgcg gcctggtgcg
     6841 cttgatggaa gccaacccga acgccgggat cattcagtcg tcgccgaaag cgtccggtat
     6901 ggatacgctg tatgcgcgct gtcagcagtt cgcgacccgc gtgtatgggc cactgtttac
     6961 agccggtttg cacttctggc aacttggcga gtcgcactac tggggacata acgcgattat
     7021 ccgcgtgaaa ccatttatcg agcactgcgc actggctccg ctgccgggcg aaggttcctt
     7081 tgccggttca atcctgtcac atgacttcgt agaagcggcg ttgatgcgcc gtgcaggttg
     7141 gggggtctgg attgcttacg atctcccggg ttcttatgaa gaattgccgc ctaacttgct
     7201 tgatgagcta aaacgtgacc gccgctggtg ccacggtaac ctgatgaact tccgtctgtt
     7261 cctggttaag ggtatgcacc cggttcaccg tgcggtgttc ctgacgggcg tgatgtctta
     7321 tctctccgct ccgctgtggt ttatgttcct cgcgctctct actgcattgc aggtagtgca
     7381 tgcgttgacc gaaccgcaat acttcctgca accacggcag ttgttcccgg tgtggccgca
     7441 gtggcgtcct gagctggcga ttgcactttt tgcttcgacc atggtgctgt tgttcctgcc
     7501 gaagttattg agcattttgc ttatctggtg caaaggaacg aaagaatacg gcggcttctg
     7561 gcgcgttaca ttatcgttgc tgctggaagt gctgttttcc gtgctgctgg ctccggtacg
     7621 catgctgttc catacggtct tcgttgtcag cgcgttcctt ggctgggaag tggtgtggaa
     7681 ttcaccgcag cgtgatgatg actccacttc atggggcgaa gcgttcaaac gccacggctc
     7741 acagctgctg ttggggttag tgtgggctgt tgggatggcg tggctggatc tgcgtttcct
     7801 gttctggctg gcaccgattg tcttctcgtt gatcctgtca ccgtttgttt cggtgatttc
     7861 cagccgtgcc accgttggtc tgcgcaccaa acgctggaaa ctgttcctga tcccggaaga
     7921 gtattcaccg ccgcaggtgc tggttgatac cgatcggttc cttgagatga atcgccaacg
     7981 ctcccttgac gatggcttta tgcacgcagt gtttaacccg tcatttaacg ctctggcaac
     8041 cgcaatggcg acagcgcgtc accgcgccag taaggtgctg gaaatcgccc gtgaccgcca
     8101 cgttgaacag gcgcttaacg agacgccaga gaagctgaat cgcgatcgtc gcctggtgct
     8161 gctaagcgat ccggtgacga tggcccgtct gcatttccgc gtctggaatt cccctgaaag
     8221 atattcttca tgggtgagtt attacgaagg gataaagctc aatccactgg cattgcgtaa
     8281 accggatgcg gcttcgcaat aaaaacgtag ttgcctgatg cgctacgctt atcaggccta
     8341 catcgttcct gcaatttatt gattttgcaa gattttgtag gtcggataag gcgttcacgc
     8401 cacatccggc aaaaacaacg agccaataaa aataccggcg ttatgccgta atgccagtca
     8461 gttaagcaac tgactggctc tttttcgggg ctgtggggta tttccagggc ctctccttta
     8521 ccactctc
//
LOCUS       DAZXUL010000161         8480 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZXUL010000161 DAZXUL010000000
VERSION     DAZXUL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.161"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            45..398
                     /locus_tag="ACH3L1_003804"
     CDS             45..398
                     /locus_tag="ACH3L1_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFT4078360.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(435..2513)
                     /gene="flhA"
                     /locus_tag="ACH3L1_003805"
     CDS             complement(435..2513)
                     /gene="flhA"
                     /locus_tag="ACH3L1_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFT4078361.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2587..3597)
                     /gene="hypE"
                     /locus_tag="ACH3L1_003806"
     CDS             complement(2587..3597)
                     /gene="hypE"
                     /locus_tag="ACH3L1_003806"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFT4078362.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3594..4715)
                     /gene="hypD"
                     /locus_tag="ACH3L1_003807"
     CDS             complement(3594..4715)
                     /gene="hypD"
                     /locus_tag="ACH3L1_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFT4078363.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4715..4987)
                     /gene="hypC"
                     /locus_tag="ACH3L1_003808"
     CDS             complement(4715..4987)
                     /gene="hypC"
                     /locus_tag="ACH3L1_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFT4078364.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4978..5850)
                     /gene="hypB"
                     /locus_tag="ACH3L1_003809"
     CDS             complement(4978..5850)
                     /gene="hypB"
                     /locus_tag="ACH3L1_003809"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFT4078365.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5854..6204)
                     /gene="hypA"
                     /locus_tag="ACH3L1_003810"
     CDS             complement(5854..6204)
                     /gene="hypA"
                     /locus_tag="ACH3L1_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFT4078366.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6416..6877
                     /gene="hycA"
                     /locus_tag="ACH3L1_003811"
     CDS             6416..6877
                     /gene="hycA"
                     /locus_tag="ACH3L1_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFT4078367.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7002..7613
                     /gene="hycB"
                     /locus_tag="ACH3L1_003812"
     CDS             7002..7613
                     /gene="hycB"
                     /locus_tag="ACH3L1_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFT4078368.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7610..8480
                     /locus_tag="ACH3L1_003813"
                     /pseudo
     CDS             7610..8480
                     /locus_tag="ACH3L1_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT         1898 a         2304 c         2284 g         1994 t
ORIGIN      
        1 gattaagatg taatataaat gcatcttaat gaggtggagg cagcatgtcc ggcaagcgta
       61 tctctcgtga aaaactgacg attaaaaaaa tgatcgatct ttatcaagcg aaatgcccgc
      121 aggcgtcagc ggagccggag cattacgagg cgttgtttgt ttacgcgcaa aagcggctgg
      181 ataaatgtgt gttcggcgaa gagaaaccag cctgtaagca gtgtccggtg cactgttacc
      241 agcctgcaaa gcgtgaagag atgaaacaga ttatgcgttg ggccgggccg agaatgctgt
      301 ggcgtcatcc gatcttaacc gtgcgtcatc tcatcgatga caagcgtccg gtgccagaac
      361 tgccggaaaa atatcgtccg aaaaaacctc gtgaatagta tgccggacag gcagatctgt
      421 ccggcaattt gcagttaaat caatgccgat ttatcaattc ccagccgctt catccgtgac
      481 agcagggtcg tgcgtttcag ccccagacgt tgcgcagcgc ctttaggccc ggcaaccacg
      541 ctgttggttt ctttcagcac gcgcacaatc aactgatatt catcttcgcc ctcctgggcg
      601 acaaccgttg cggcaggcgg cgtttcaggc tccggtaaag caatatctgg caatgacagc
      661 tgcagcacgt tgccgcgtgt tagcaatact gcgcgctcaa tgacgttttc cagttcgcgt
      721 acgttacccg gccactccat gttgctcaag gtgcgcagcg tctcggcagg aatgctgtcg
      781 atattgcgcc ccagacgacg ggcaattttg aaggtaaagg ctttcgccag cagcggaata
      841 tcttccggac gctcgcgtag tggcggcagg tgaatcggga atacgttcag gcggtaatag
      901 agatcgctac ggaactctcg gtcggcgacc atttttttca gatcgcggtt agtcgcggca
      961 attaaacgta cgtccgtctg aatgattttg ttgctgccga gacgttcaaa ctcctgttcc
     1021 tgcaatacac gcagcaactt cggctgtaac tccagtggca tatcgcccac ttcgtcgagg
     1081 aacagagagc ttttatccgc cagttcaaaa cgaccaatgc gctgggcgtt ggcaccggta
     1141 aaagccccac gctcatgacc aaacagatcg ctttccagca atccggcagg catcgccgcg
     1201 cagttcattt tgaccatgcg gcgattatta cgcccactga gattatggat cgcacgggca
     1261 atcagctctt tacccgagcc agtttcaccg aggatcagca cggtactgtc actttgcgcc
     1321 accatttcaa cttgtttaag cacgctgtac atggcttcgc tgcggccaat aatctcgcca
     1381 aattcactat caacattgtt gagctgctcg gtcagggcga ggttttcatc aaccagccgt
     1441 tctttcagac gatggatttc ctgataggcg agggcgttat cgacagcgat tgccacacgt
     1501 tcggcaatct ggcgcagcaa attcagattg gtagtggtaa acactttctc ttcgcattgc
     1561 gccagtttca gcacgcccag catggtgtcg ccagacatca gcggtaacag gcacaaggtt
     1621 tgaatctggt tgccccaggt gtcgaacaac atgcgttcat agggggctaa atcgtcccgc
     1681 tcgtagagat tgatcagcag catctcttta cttttgaaca cgcgttcggt gagggttccg
     1741 gcttcatcga cttcgctctg ttcgtgggcg ggatgttgtt tatcaagata gtgagtggag
     1801 tagatgttga gtttgttttt acggtggctg cgtaagacaa tgctgatatc gtcaatgtcg
     1861 aaatagtaat ggatttcttt ggcgacttcg ctgaccagtt cgtccatatc caggcgggaa
     1921 agcaccgcgt tggtaatggc aaccaggatg cggaagttat cgcgctcccg gcataacaac
     1981 tcatagtcga cattgttgac gacgcggctc tggatttgtt cggtgacgac agaaacgatc
     2041 tgcgtaaatg tttgcagacg attgaactct ttttcgctcc agggacgctc gtcataacga
     2101 ataaattcac agccaccaaa aatatgccct cccgccgcca gtggcatcag gcaatagtga
     2161 ccaaatttgg ggtatagccc acctgcggcc agctgcggcc aggtttcaca aaattcttcg
     2221 taactgcaat gcagcgtatc agggcgcgac aaaatgctgc gtaccggacc gtgtgccaga
     2281 acagtttcgt cttcatattt aatgggggtg tctttttcac gcgacgcgta ataagacgca
     2341 cgttgagtct gcgcttgcca caacacaata gccgcgttgt cggcgagcgc agaacgcttt
     2401 accagttgcg aaagagcctc acacagcgag gccagatcgg gctgctgcaa tagtgtccga
     2461 gtgatgtcga acaacccttg ctgtccgaga tcactcatcg gtgtatatga cattttgttc
     2521 ttccaggaaa gcaccgcaaa gcggtgcaaa aggtcattga ctaactatag gagatttaga
     2581 attttattag catatacgcg gaagcggttc ggcgtgtggt aaatcgaggg ttcgtttcac
     2641 gccatacaga ccggcaagac gaacaccttt acgttccacc acttcaccaa tcagcgccgc
     2701 gtctttcccc agtggatggg aatgtaacgc tgccagcact tgctctgccg cgttgcgttc
     2761 aacggcgatc accagtttgc cttcgttggc aaagttcagg gcgtccagtc ccagcaattc
     2821 gcaaacgccg cgcacggcag gtttaaccgg cagcgctgat tcagaaattt caataccaca
     2881 accgcaggct gccgcgaact catgaaccac cgcgtttaca ccaccacggg tggcatcacg
     2941 cagcgctttc acgccgggaa tgtcacgcag cgtctgaata agcggcgtca gcaccgcgca
     3001 gtcgctgacc agttcgccat ccagccccag ctgctcacgc aggttaagga tagtcgcccc
     3061 gtggtcgccg agtgtaccgc taaccagcaa tacatcgcct gcggttagcg tctgtgcgcc
     3121 ccagtgaata ttcgccggaa ttgcgcccat gccagcggtg ttgataaaca gtttatctgc
     3181 cgcgccgcgc tgcaccactt tagtatcgcc agtaacgatg gcaatgcctg ccgtgcgggc
     3241 ggtttctgcc atgctggtca ctacggcttt cagtgtctcc atcggcaatc cttcttcgag
     3301 gataaagcca caggagagat agcgcggaat agcgccactg cccgcaacgt cattcgcggt
     3361 gccgcaaatc gccagcttgc cgatattacc gccaggaaag aacagcgggt caatgacgta
     3421 actgtcggtg gagaacgcca gacggtcgcc ttccgctacc agctgcgcca gatcaagacg
     3481 tgcctgatct tcctgctctg ccagccacgg gttggcaaag gcttccataa acaggctgtt
     3541 gattaattgc tgcatcgcct ggccgccgct gccgtgggcg agttggatat tattcacgct
     3601 tcactctcct gctgacgata ctgataccac gcggcgcacg ctccttcgga ggagaccatc
     3661 agtgcaccaa atgcggtttg aggattacag gtgttaccaa acagcgggca ttgatgcggc
     3721 ttacatttgc ccgttaatac ctcaccacaa cgcgcgcgcg ggtcatcgca gacctgctgc
     3781 ggtgccgggc ggaaatgtgc ttcggcatcg aatcgttgat aatccggcgt caggtgcacg
     3841 ccagaagatt caatcacgcc taagccgcgc cattcgctgt cgccgttgac acagaacaca
     3901 tcggcaatcg cctgttgcgc cagtaggtta ccggcatccg gtaccacccg acgatactga
     3961 ttctctacct tgctgtgggc cgctattttc tgctccacca gcatgaccac gccttgcagt
     4021 agatcaaggg gttcgaaacc agcgaccacc agcggacgat gaaaatcgct ggcgataaaa
     4081 ttataggcat cagtgccgat aaccatacta acgtggcccg gcgcgaggaa cgcgtcgata
     4141 ccgttatccg gctgttccag caaactgcgc agcgtcggga taagcgtaat gtgctggcag
     4201 aagaagtaaa aattctgcac atcacgcgct ttcgcctgtt gcagagtgat ggcggtggtc
     4261 ggcatggtgg tttcaaaacc taagccgaag aacaccactt tgcgggttgg attctcctgc
     4321 gccagtttca acgcatccat cggcgagtaa acgatgcgca catcggcacc gcgtgctttt
     4381 gcctgcaaca gcgatccctg tttccctggt acgcgcatgg cgtcgccaaa ggtacagaag
     4441 atgacttccg gatggctggc aatctccacg caggtgtcga ttctacccat cggcagtacg
     4501 cacaccgggc aacccggacc gtggataaac tcaacgtttt caggcagtaa ctggtcgagg
     4561 ccgaatttaa aaatggcgtg ggtatgaccg ccacacactt ccataatccg cagagggcgt
     4621 tcggcggtgt aagagagatg tgaagcacgt tcgcgcagat gctcaattaa ctgcatcacc
     4681 tgttccggcg cgcgatattc atcaacaaaa cgcattattt ttcctcgcca tacaacagcg
     4741 cgccgacatc cggctcaacg tcaaacatgt tttgtaaggc gtcgagagtg tcgcgtgctt
     4801 cggcttcatt aattacgctc atggcaaagc caacgtgaac cagtacccac tggcccacgc
     4861 gcggctgacc gttttcatcg cagctgccga ctaacgttaa atcgacatcg cgctgaatgc
     4921 cgcagacgtc gactttcgcc tggttgccgt caatggtgcg gatctggccg ggaacgccta
     4981 tgcacatcgc tgtgtctcca gccagttcag ccactggtcc atcccttcgc cgctggtggc
     5041 ggaaataagg atgatttcaa tttctggatt gacttcgcgg gcgcaggcga tgcacttctc
     5101 aacgtcaaag ttgagatacg gcaacaggtc aactttgttg agcagcatca gcgaggcggc
     5161 ggcaaacata tgcggatatt tcagcggttt gtcttcacct tcggtaacgg aaagcaccgc
     5221 tactttgtgt ttttcaccga gatcgaagct ggccgggcat acgaggttgc caacgttttc
     5281 gataaacaga ataccgttat cgtccagtgg cagacgcggt gcggcgtcgg caatcatctg
     5341 tgcgtcaaga tggcagcctt taccggtgtt cacctgaatc gctggtgtac cggtagcgcg
     5401 aatgcgtgcg gcatcgttca cggtttgctg gtcgccttca ataaccgcgc acggaacgct
     5461 gtctttcagg cgcattaagg tttccgtcag cagggtggtt ttaccggaac cagggctgga
     5521 aaccaggttg agcaccagtt gcttgcgggc agcaaagcgc gcgcggttgc gttcagccag
     5581 acggttgttt ttgtccagca cgtcaatttc gacttccagc atccgacgct ggctcatgcc
     5641 cggtgtgtga gtgcccgctt cgccatgacc gtaatgcagg tcgccttctt cagtctggct
     5701 gggggtaaat tcaggcgctt taatgccggt gattttcatc tgcgggcgtg ccgccggggc
     5761 aaatggcgcg ctacgaaacg cggaatgagg gttatgttca tcaccctcga tatacaggtt
     5821 gccttcaccg caaccgcatg ttgtacacat cgctcactcc tggtctattt ctatccgccg
     5881 aatctgtaaa ccgtcgtctg ccacaatctg tagcatgtca ccatgacact gtggacagcg
     5941 gcggacgcgc tgggtcagta gcgtcacata ctgttggcat gtttcacacc agcattcggc
     6001 ctcttgttct tcgaggtgca gtttacaacc ttccgccacg ctgccgcggc aaaccagatc
     6061 aaaacaaaag gcaagagagc tggtttcgac acaagaaaat gcgccaattt tgagccagac
     6121 cccagttacg cgttttgcgc cgtgttttgc ggcctgctgt tcgatcaatt ccagtgcccg
     6181 ttggcagagg gttatttcgt gcatatcgcc tcccattaac tattgccagc tacaagcaat
     6241 aattgcgcca gtgttgatta tccctgcggt gaataatgtc gatgatgtcg aaatgacacg
     6301 tcgacacggc gacgaaattc atctttagct taaaaatctc tttaataaca ataaattaaa
     6361 agttggcaca aaaaatgctt aaagctggca tctctgttaa acgagtaacc tgacaatgac
     6421 tatttgggaa ataagcgaga aagccgatta catcgcacag cggcatcgtc gcctacagga
     6481 ccagtggcac atctactgca attcgctggt tcaggggatc acgttatcga aagcgcgcct
     6541 gcatcacgcc atgagctgcg cgccggacaa agaactctgt ttcgtccttt ttgaacattt
     6601 tcgtatttac gtcaccctgg cggatggctt taacagccac accatcgagt attacgtcga
     6661 aacaaaagat ggcgaagaca aacagcggat tgcgcaggcg caactgagca ttgacggcat
     6721 gattgatggc aaggtcaaca tccgcgatcg cgaacaggtt ctggaacact atctcgaaaa
     6781 aatcgctggc gtttacgaca gcttatacac cgccattgaa aacaatgtgc cggtgaattt
     6841 aagccaactg gtaaagggac aaagcccggc agcatgagct gaggctttgc ccgttttgca
     6901 ggcgttacgc ctgtttgggg atgggcgtgt cgatgagtgt cgaaaatgac atttcatcgg
     6961 catgttttcg tcaaaaatga caatcacctg aggaatgcct ggtgaatcgt tttgtaattg
     7021 ccgactccac gctctgtatc ggctgccaca cctgtgaggc cgcctgttca gagacgcatc
     7081 gccagcacgg cctgcaatca atgccgcgcc tgagagtgat gctcaatgaa aaagaatctg
     7141 cgccgcagct ctgtcaccac tgtgaagatg caccctgcgc ggtggtctgc ccggttaacg
     7201 ccatcacccg cgttgatggg gccgtgcagt tgaatgaaag tctgtgcgta agctgcaaac
     7261 tgtgcggcat cgcctgcccg tttggcgcaa ttgaattttc cggcagccgt ccgctggata
     7321 ttccggcaaa cgccaatacc ccgaaagcgc caccggcacc gcctgctccg gcgcgtgtca
     7381 gcacattgct tgactgggtg ccaggtattc gcgcgatcgc cgtcaaatgt gacctttgta
     7441 gctttgatga acaaggtccg gcctgtgtgc ggatgtgccc gactaaagcc ctgcatctgg
     7501 tggataacac cgatatcgcc cgcgtcagca aacgtaagcg tgagctgacc tttaacacgg
     7561 actttggcga tctcaccttg tttcagcagg ctcaaagtgg agaggctaaa tgagcgcaat
     7621 ttccctgatc aatagcggcg tggcatggtt tgtcgccgcc gctgttctgg catttctctt
     7681 ttcaaaaagc gctaagtggc tggatagccg gaattggcgg cgcggttggt agtctgtata
     7741 cggcagccgc gggcttcact gtactgactg gcgcggttgg cgtgagcggt gcgctgtcgc
     7801 tggtaagcta cgatgtgcaa atctccccgc ttaacgcgat ttggctgatt accctcggcc
     7861 tttgcggtct gtttgtcagc ctctataaca ttgactggca tcgccacgcg caggtgaagt
     7921 gcaacggctt gcagatcaat atgttgatgg ctgccgccgt ctgcgccgtc atcgccagca
     7981 acctcggcat gttcgtggta atggccgaag tcatggcctt gtgtgcggtg ttcctcacca
     8041 gcaacagcaa agagggcaaa ctgtggtttg cgctgaggcg tcttggcact ctgctgctgg
     8101 tgattgcttg ctggctgctg tggcagcgtt atggcacgct ggatctgcgc ctgctggata
     8161 tgcgtatgca acagctgccg ctcggttccg atatctggct gctcggtgtg attggctttg
     8221 gcctgctggc tgggattatt ccgctgcacg gctgggtgcc gcaggcacat gctaacgcct
     8281 ctgcgccagc tgccgcgttg ttttctacgg tagtcatgaa aattggcctg ctgggcatgt
     8341 taaccctgtc actgctgggc ggtaatgcac cgctgtggtg ggggatcgcg ctgctggtgc
     8401 tcggcatgat caccgcgttc gtcggtggtc tgtatgcgtt gatggaacac aacatccagc
     8461 gcctgctggc ttaccacacc
//
LOCUS       DAZXUL010000162         8438 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZXUL010000162 DAZXUL010000000
VERSION     DAZXUL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.162"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            356..1288
                     /gene="glaH"
                     /locus_tag="ACH3L1_003814"
     CDS             356..1288
                     /gene="glaH"
                     /locus_tag="ACH3L1_003814"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFT4078369.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1308..2576
                     /gene="lhgO"
                     /locus_tag="ACH3L1_003815"
     CDS             1308..2576
                     /gene="lhgO"
                     /locus_tag="ACH3L1_003815"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFT4078370.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2599..4047
                     /gene="gabD"
                     /locus_tag="ACH3L1_003816"
                     /pseudo
     CDS             2599..4047
                     /gene="gabD"
                     /locus_tag="ACH3L1_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            4055..5341
                     /gene="gabT"
                     /locus_tag="ACH3L1_003817"
                     /pseudo
     CDS             4055..5341
                     /gene="gabT"
                     /locus_tag="ACH3L1_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            5579..6979
                     /gene="gabP"
                     /locus_tag="ACH3L1_003818"
     CDS             5579..6979
                     /gene="gabP"
                     /locus_tag="ACH3L1_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFT4078371.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7000..7662
                     /gene="csiR"
                     /locus_tag="ACH3L1_003819"
     CDS             7000..7662
                     /gene="csiR"
                     /locus_tag="ACH3L1_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFT4078372.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7663..8112)
                     /gene="kbp"
                     /locus_tag="ACH3L1_003820"
     CDS             complement(7663..8112)
                     /gene="kbp"
                     /locus_tag="ACH3L1_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFT4078373.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8196..8354)
                     /locus_tag="ACH3L1_003821"
     CDS             complement(8196..8354)
                     /locus_tag="ACH3L1_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFT4078374.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1914 a         2208 c         2335 g         1981 t
ORIGIN      
        1 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
       61 atgtttagtt tactaacggt cattttgtag tgaagccatt tactgttttt tatcgaccag
      121 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
      181 ctcactttgc cttaataccc tacgttaaat gttactaatt tgttgctttt gatcacaata
      241 agaaagcaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
      301 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
      361 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
      421 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
      481 caaacagttt ctcgaacagg ttgccgaatg gcccgtgcag gcgctggagt acaaatcgtt
      541 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
      601 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtaggtat
      661 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
      721 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
      781 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
      841 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgactggga
      901 acatctggac cactatttcc gccatccact ggcgcgtcgc ccgatgcgct ttgccgcgcc
      961 gccgagcaaa aacgtcagca aagatgtttt ccatccagtg ttcgacgttg atcaacaggg
     1021 tcgcccggtg atgcgctata tcgaccagtt cgtccagcca aaagattttg aagaaggcgt
     1081 gtggttgagc gagctttccg atgccattga aaccagcaaa ggcattcttt ctgtacccgt
     1141 tcccgttggc aaattcctgt tgataaacaa cctgttctgg ctgcacggtc gcgaccgctt
     1201 tactccgcac ccggatctgc gccgtgaact gatgcgtcag cgtggctatt tcgcttacgc
     1261 cactcaccac taccagacgc atcagtaagc gcaaaggaat tgagcggatg tatgattttg
     1321 tgattattgg cggcggcatc atcggcatgt cgaccgccat gcaactgatt gatgtttatc
     1381 cggatgcccg cattgcgttg ctggaaaaag agtccggccc ggcctgtcac cagacgggcc
     1441 acaacagcgg cgtgatccat gccggggtct attacacgcc cggcagcctg aaggcgcagt
     1501 tttgcctggc ggggaaccgt gccaccaaag ccttttgcga tcaaaacggc attcgctatg
     1561 acaactgcgg caagatgctg gtcgccacgt ctcagctcga aatggaacgg atgcgcgcct
     1621 tatgggaacg cacagcggcg aacggtatcg agcgcgagtg gataaacgcc gtggaactgc
     1681 gcgagcgcga accgaatatc accgggcttg gtggcatttt tgtgccgtcc agcggcattg
     1741 tcagctaccg cgaagtgacg gcggcaatgg caaaaatctt ccaggccaga ggcggcgaga
     1801 ttatttataa cgccgaagtc agcgccctca gtgagcataa aaacggcgtg gtgatacgca
     1861 cccgtcaggg cggtgaatat gaagcatcga cgctgattag ctgttccggg ctgatggctg
     1921 accggctggt gaaaatgctc ggcctcgaac cgggctttat tatctgcccg ttccgtggcg
     1981 agtatttccg ccttgcgccg gagcataacc agattgttaa ccacctgatt taccccattc
     2041 ccgaccccgc gatgccattt ttgggcgtgc atctcacccg catgatcgac ggcagcgtga
     2101 ccgtcgggcc aaacgcggtg ctggctttca aacgcgaagg ctatcgcaag cgcgacttct
     2161 catttagtga cacgctggag attttgagct cgtcggggat tcgccgggtg ctgcaaaacc
     2221 atctacgctc aggactgggc gagatgaaaa actcgctgtg caaaagcggc tatctgcggc
     2281 tggtgcaaaa gtattgtccc cggctttcgt taagcgatct ccagccctgg cccgccggtg
     2341 tgcgggcgca ggcagtatcg ccggacggca agctgattga cgattttctg tttgtcacca
     2401 ccccacgcac gatccacacc tgcaatgcgc cctccccggc agcgacatca gcaattccta
     2461 ttggtgcgca tattgtcagc aaggtacaaa cgctgttggc aagccagagt aaccccggac
     2521 gcacgctgcg agcggcacgt agtgtggatg ccttacacgc cgcatttaat caataacctt
     2581 tgaaaacagg acgtaacaat gaaacttaac gacagtaact tattccgcca gcaggcgttg
     2641 attaacgggg aatggctgga cgccaacaat ggtgaagtca tcgacgtcac caatccggcg
     2701 aacggcgaca agctgggtag cgtgccgaaa atgggcgcag atgaaacccg cgccgctatc
     2761 gacgccgcca accgcgccct gcccgcctgg cgtgcgctca ccgccaaaga acgcgccacc
     2821 attctgcgca actggttcaa tttgatgatg cagcatcagg acgatttagc gcgcctgatg
     2881 accctcgaac agggtaaacc gctggctgaa gcgaaaggcg aaatcagcta cgccgcctcc
     2941 ttcattgagt ggtttgctga agaaggcaaa cgtatttatg gcgacaccat tcctggtcat
     3001 caggccgata agcgcctgat tgttatcaag cagccgattg gcgtcaccgc cgccatcacg
     3061 ccgtggaact ttccggcggc gatgattacc cgtaaagccg gtccggcgct ggcggcaggc
     3121 tgcaccatgg tgctgaagcc cgccagtcag acgccgttct ctgcgctggc gctggcggaa
     3181 ctggcgattc gcgcgggcat tccggctggc gtgtttaacg tggtcaccgg ttcggcaggc
     3241 gtggtcggta atgaactgac cagcaacccg ctggtacgca aactgtcgtt taccggttag
     3301 accgagattg gccgccagtt aatggaacag tgcgcgaaag acatcaaaaa agtgtcgctg
     3361 gagctgggcg gcaacgcgcc gtttatcgtc tttgacgatg ccgatctcga taaagccgtg
     3421 gaaggcgcgc tggcctcgaa attccgcaac gccgggcaaa cctgcgtctg cgccaaccgt
     3481 ttatacgtgc aggacggcgt gtatgaccgc tttgccgaaa aattacagca ggcggggagc
     3541 aaactgcaca tcggcgacgg gctggagaaa ggcgtcacca tcgggccgct gatcgatgaa
     3601 aaagcggtag caaaagtgga agagcatatt gccgatgcgc tggagaaagg tgcgcgtgtg
     3661 gtttgcggcg gtaaagcgca cgaacgcggc ggcaacttct tccagccaac cattctggtg
     3721 gacgttccgg ccaacgccaa agtgtcgaaa gaagagacgt tcggccccct cgccccactg
     3781 ttccgtttta aagatgaagc cgatgtgatt gcacaagcca atgacaccga gtttggtctt
     3841 gccgcctatt tctacgcccg tgatttaagc cgcgtcttcc gcgtcggcga ggcgctggag
     3901 tacggcatcg tcggcatcaa taccggcatt atttccaatg aagtggcccc attcggcggc
     3961 atcaaagcct cgggtctggg tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa
     4021 atcaaatata tgtgcatcgg tctttaactg gagaatgcga aggaacagca ataaagagtt
     4081 aatgcagcgc cgcagtcagg cgattccccg tggcgttggg caaattcacc cgattttcgc
     4141 tgaccgcgcg gaaaactgcc gggtgtggga cgtttaaggc cgtgagtatc ttgatttcgc
     4201 gggcgggatt gcggtgctca ataccgggca cctgcatccg aaggtggtgg ccgcggtgga
     4261 agcgcagttg aaaaaactgt cgcacacctg cttccaggtg ctggcttacg agccgtatct
     4321 ggagctgtgc gagattatga atcagaaggt gccgggcgat ttcgccaaga aaacgctgct
     4381 ggttacgacc ggttccgaag cggtggaaaa cgcggtaaaa atcgcccgcg ccgccaccaa
     4441 acgtagcggc accatcgctt ttagcggcga gtatcacggg cgcacgcatt acacgctggc
     4501 gctgaccggc aaggtgaatc cgtactctgc gggcatgggg ctgatgccgg gtcatgttta
     4561 tcgcgcgctt tatccttgcc cgctgcacgg cataagcgag gatgacgcta tcgccagcat
     4621 ccaccggatc ttcaaaaatg atgccgcgcc ggaagatatc gccgccatca tgattgagcc
     4681 ggttcagggc gaaggcggtt tctacgccgc gtcgccagcc tttatgcagc gtttacgcgc
     4741 tctgtgtgac gagcacggga tcatgctgat tgccgatgaa gtgcagagcg gcgcggggcg
     4801 taccggcacg ctgtttgcga tggagcagat gggcgtggcg ccggatctta ccacctttgc
     4861 gaaatcgatc gcaggcggtt tcccgctggc gggcgtcacc gggcgcgcgg aagtgatgga
     4921 cgctgttgca ccgggcggtc tgggcggcac ctatgccggt aacccgattg cctgcgtggc
     4981 tgcgctggaa gtgttgaagg tgttcgagca ggaaaatctg ctgcaaaaag ccaacgatct
     5041 ggggcagaag ctaaaagacg gattgttggc gatagccgaa aaacacccgg agatcggcga
     5101 cgtacgcggg ctgggatcga tgattgccat cgagctgttt gaagatggcg atcacagcaa
     5161 accggacgca aaactcaccg ccgagatcgt ggcacgcgcc cgcgataagg gcctgattct
     5221 tctctcctgc ggcccgtatt acaacgtgct gcgcatcctt gtaccactca ccattgaaga
     5281 cgctcagatc cgtcagggtc tggagattat cggtcagtgt tttgatgagg cgaagcagta
     5341 gcaccgtccc catgcaggag gcgacgctgc gcgtcttgtc cggtctacga gaatccaagt
     5401 cggataaggc gtttacgccg catccgacaa tctgtacgtg aacaggaata aatctatgtc
     5461 ggccggataa ggcggagccg ctctccggca aaaagaatca ataacaatta aacgcgtgac
     5521 ccgacgcggg aaatgtcggg gcgctctccc aagtgacaca ctttcgagag gattcaggat
     5581 ggggcaatca tcgcaaccac acgagttagg cggcgggctg aaatcacgcc acgtcaccat
     5641 gttgtctatt gccggtgtta tcggcgcaag tctgtttgtc ggttccagcg tcgccatcgc
     5701 cgaagcggac ccggcggtat tactggccta tctgttcgcc ggattactgg tggttatgat
     5761 tatgcggatg ctggcggaaa tggcagttgc cacgcccgat accggttcgt tttccaccta
     5821 tgccgataaa gccattggcc gctgggcggg ctataccatc ggctggttgt actggtggtt
     5881 ttgggtactg gttatcccac tggaagccaa catcgccgcc atgatcctgc actcgtgggt
     5941 tccaggcatt cccatctggt tattttccct cgtcattacc ctcgccttaa ctggcagtaa
     6001 tttattaagc gttaaaaact acggcgaatt tgagttctgg ctggcgctgt gcaaagtcat
     6061 cgctatcctg gcctttattt tccttggtgc agtcgcaatt agcggttttt acccttatgc
     6121 cgaagtgagc gggatctcaa gattgtggga tagcggcggc tttatgccca acggtttcgg
     6181 tgcggtatta agcgcgatgt tgatcaccat gttctcgttt atgggcgcag aaattgtcac
     6241 cattgccgcc gcggaatccg acatgccgga aaaacatatt gtccgcgcca ccaactcggt
     6301 tatctggcgt atttctatct tctatttgtg ctctattttt gtcgtcgtgg cattaatacc
     6361 gtggaatatg ccggggctga aagccgttgg atcttatcgc tcggttctgg aattgctcaa
     6421 tattccccat gcgaaattaa tcatggactg tgtgatatta ctttccgtaa ccagttgcct
     6481 gaactcggcc ctgtataccg cctcaaggat gctctactcc ttaagccgtc gcggtgatgc
     6541 tcccgcggta atgggtaaaa tcaaccgcag taaaaccccg tacgtggcgg tgttactctc
     6601 caccggcgca gcattcttaa cggtggtggt gaactattac gcgcctgcga aggtatttaa
     6661 atttctgatc gacagctccg gcgctatcgc cctgctggtt tatttagtca tcgccgtttc
     6721 acagttgcgg atgcgcaaaa ttctgcgagc agaaggaagc gaaattcgct tgcgaatgtg
     6781 gcttgatccc tggctcacct ggctggtcat cggctttatt acctttgtgt tggtagtgat
     6841 gctattccgt ccggcgcaac agttagaagt gatctccacc ggcttattag cgatagggat
     6901 tatctgtacc gtgccaatta tggctcgctg gaaaaagctg gtattgtggc aaaaaacacc
     6961 cgttcataat acgcgctgat aatgatcagg agtcacacca tgaccattac gtctctggat
     7021 ggctatcgct ggctgaagaa cgatattatt cgcggtaatt ttcaaccgga tgaaaaatta
     7081 cggatgagtt tactgacatc gcgttatgca cttggcgttg ggccgttgca ggaagcactt
     7141 tctcaactgg tggcggaacg gctggtcacg gtggtgaatc aaaaagggta tcgggtggcg
     7201 tctatgtcag agcaggagct gctcgatatt ttcgacgccc gcgccaatat ggaagcgatg
     7261 ctggtcagtc tggcgattgc ccgcggtggc gatgaatggg aggcagacgt tctcgcaaaa
     7321 gcgcatctgc tcagtaagct ggaagcctgt gacgccagcg agaaaatgct tgatgagtgg
     7381 gatctgcgtc atcaggcgtt tcatacagca attgtggcgg gctgtggttc tcactatttg
     7441 ctgcaaatgc gtgaacggtt gtttgatctg gcggcgcgtt atcgatttat ctggctgcgg
     7501 cgaacggtgc tttcggtgga aatgctggag gataaacacg atcagcacca gaccctgact
     7561 gcggcggtac tgccgcgaga tacagcgcgc gccagtgagt taatgcgcca gcatttactg
     7621 acgccaattc ccattatcca gcaggcgatg gctggcaatt aactactctt ccggaatacg
     7681 caacacttgc cccggataaa ttttatccgg gctttttagc atcggtttat tcgcttcgaa
     7741 gattttattg tacagattag cgttaccgta gacctgtttg gaaatggcac tcagagtgtc
     7801 gccagactta acggtataaa actggctggc agtggctggt gtcgccgttt tcacctgatc
     7861 atcaacactg gcaataccgg aaatattccc caccgcaaca aggattttct ccttcgcctc
     7921 ctgactcagg ccgtcaccag tgaccgtcgc tttgccgtcg gcaatttgaa tgttcacttt
     7981 atcggcatca ggtataccgg ttttgctcag atgctcctgc accttcttcg cctgatcgtc
     8041 tttatcgtgc tgacctgtaa ccgcatccca gagtttttct ccggcatctt taacaaaatt
     8101 gaacagaccc atagctacct cattcgttaa cggaaacact cagaaagtgt agcagaggaa
     8161 cgcggcgcgt taacgccgcg ggggtgagca caggtttaat cgcgggtttg cacccagaac
     8221 gcgtgaatca aaccggggat atacccaagc agtgtcagca gaatattaat aatgaacgcc
     8281 caaccgaacc ctttaccgag cagcacgccg atcggcggca gaatgatggt gatgacgatt
     8341 ctccagaaac ccatatgtac tccctataag aaaattactc attgtttaaa aagagatttt
     8401 atctcttaag cgtagtaatt ttagcggagc ctgccagt
//
LOCUS       DAZXUL010000163         8332 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZXUL010000163 DAZXUL010000000
VERSION     DAZXUL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.163"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(168..566)
                     /locus_tag="ACH3L1_003822"
     CDS             complement(168..566)
                     /locus_tag="ACH3L1_003822"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFT4078375.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="ACH3L1_003823"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="ACH3L1_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFT4078376.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..6128
                     /gene="traI"
                     /locus_tag="ACH3L1_003824"
                     /pseudo
     CDS             875..6128
                     /gene="traI"
                     /locus_tag="ACH3L1_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="ACH3L1_003825"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="ACH3L1_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFT4078377.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="ACH3L1_003826"
     CDS             6949..7509
                     /locus_tag="ACH3L1_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFT4078378.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="ACH3L1_003827"
     CDS             7640..7852
                     /locus_tag="ACH3L1_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFT4078379.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8096..>8332
                     /locus_tag="ACH3L1_003828"
     CDS             8096..>8332
                     /locus_tag="ACH3L1_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFT4078380.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2086 c         2671 g         1662 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc cagtgcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggggc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 agtcgatttt ttatattttg tgattagttt cacaaaataa ggtgttattc aatgtgtgct
     8041 gcactattca ggggggctga aatacaggtg gcatttatct gatatggaga ataacatgag
     8101 gaaatatatt ccactggcat tatttatctt ttcatggccg gtattgtctg cagatattca
     8161 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cactgaaggc
     8221 ggtgcggatc aggctggtaa atattgatgc accggaaaag aaacaggatt atggccggtg
     8281 gtcaacagac atgatgaaat cactggtcgc aggaaaaacg gttaccgtca ca
//
LOCUS       DAZXUL010000164         8309 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZXUL010000164 DAZXUL010000000
VERSION     DAZXUL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.164"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            82..390
                     /locus_tag="ACH3L1_003829"
                     /pseudo
     CDS             82..390
                     /locus_tag="ACH3L1_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            362..2289
                     /locus_tag="ACH3L1_003830"
                     /pseudo
     CDS             362..2289
                     /locus_tag="ACH3L1_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2273..2476
                     /locus_tag="ACH3L1_003831"
     CDS             2273..2476
                     /locus_tag="ACH3L1_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFT4078381.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2473..4066
                     /locus_tag="ACH3L1_003832"
                     /pseudo
     CDS             2473..4066
                     /locus_tag="ACH3L1_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            4056..5561
                     /locus_tag="ACH3L1_003833"
     CDS             4056..5561
                     /locus_tag="ACH3L1_003833"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFT4078382.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5598..5945
                     /locus_tag="ACH3L1_003834"
     CDS             5598..5945
                     /locus_tag="ACH3L1_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFT4078383.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            6003..7031
                     /locus_tag="ACH3L1_003835"
     CDS             6003..7031
                     /locus_tag="ACH3L1_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFT4078384.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7083..7469
                     /gene="gpFI"
                     /locus_tag="ACH3L1_003836"
     CDS             7083..7469
                     /gene="gpFI"
                     /locus_tag="ACH3L1_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFT4078385.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7481..7858
                     /locus_tag="ACH3L1_003837"
     CDS             7481..7858
                     /locus_tag="ACH3L1_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFT4078386.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7845..>8309
                     /locus_tag="ACH3L1_003838"
     CDS             7845..>8309
                     /locus_tag="ACH3L1_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFT4078387.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1936 a         1951 c         2558 g         1864 t
ORIGIN      
        1 gcagccttca gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt
       61 ccgctattgc cagtacacct caaactactc attgccagat gggaactgac cgcagaacag
      121 gctgtcgcac agcagttaaa aaatcaggtg tcaaaaggaa atcttatcga taccggattt
      181 tgtatttttg cccttagtaa gctggcgatg gctttatcca gtacgcttga ctccattcct
      241 ttgtccatgc aacgacagtt cccggattta acgccgcgtc atattgatca tctgaaaatc
      301 cttattgcta agggagcgaa tcagtgcgcg cgtgcggggg ataaattacc ggatttactc
      361 gatgaatata tcagaacaac aactgagtaa tatgatggtg gcagtcacta tcgcactgca
      421 gccactggtc agggtattgc cggtgacggc tgtagaatgg gccgatcaaa attattacct
      481 gcctaaagaa tcgtcatacg gtgaggggga atggaaaacg ctgcagtttc aggtcgccat
      541 catgaactgc atgggcaatg acgagattcg tacagttaac ctgattaaat ctgcccgtgt
      601 tggttataca aaaatggtgc ttggtgtgat cgggtatttt attgagcaca aatcccgcaa
      661 cactcttctt tttcagccga cggactcagc tgctgaagat tttatgaagt ctcacgtgga
      721 agcaacaatt cgtgatgttc cttgcctgaa aaaactttct ccctggctgg gccggaaaca
      781 ccgggataat acgctcacac tgaagcgttt ttcctctggt gtggggttct ggtgtttggg
      841 tggtgctgct gccaaaaact accgtgaaaa atctgtcgat gtggtctgct atgacgaact
      901 ctcctcgttt gaaccggatg tggaaaaaga aggttcgcca acactgcttg gcgataaacg
      961 tattgaaggt tcggtatggc caaaatccat acgcggttca acgccaaaaa ttaaaggctc
     1021 ctgtcagatt gaaaaagctg cgaatgaatc tgcgcatttc atgcggtttt atgtcccttg
     1081 ccctcattgc ggggaggagc agtatctgaa gtttggcgat gatgcgacgt cgtttggcct
     1141 gaaatgggag aagggtaaac cggaaacggt gtattacctg tgtgaacata atggctgcgt
     1201 gatccgtcag tctgaacttg atcagaccgg ggggcgctgg atttgtgaca ataccgggat
     1261 gtggacgtgt gacggtctga cgtttttcag tgcttcgggt aatgaaattc cgccgccgcg
     1321 ctccatcact ttccatatat ggacggcgta cagtccgttc accaccaggg tacagatagt
     1381 ctgtgactgg ctggatgcac tgaaagatcc caacggcgtg aaaacttttg tgaacaccac
     1441 gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg tactgatgga
     1501 taaggttgtg ccttacacgg caacggtgcc tgtccgggtg gtttatctga cggcgggcat
     1561 tgactcgcag cgaaaccgtt ttgagatgta tgtctggggg tgggctccgg gagaggaagc
     1621 ctttctggtg gataaaatca tcatcatggg acgtcctgat gaggaagaga cgctgttacg
     1681 tgtggatgcg gcgatcaata aaaaataccg ccatgcagac ggtaccgaaa tgaccatttc
     1741 ccgtgtctgc tgggatatcg gggggatcga tggcgaaatc gtgtatcaga ggtcaaaaaa
     1801 acacggtatt ttccgggtac tgccggtaaa aggtgcctct gtctatggca agccggtgat
     1861 caccatgccg aaaacccgca atcagcgggg tgtgtatctg tgtgaggtgg ggacggatac
     1921 cgcaaaagaa attctctatg cccgaatgaa ggccgatccc acccctgtga atgaggccac
     1981 atcgtatgcc atccgctttc ctgataatcc ggagattttt tcgcagacag aggcacaaca
     2041 actggtggcg gaggaactgg tggaaaagtg ggaaaaagga aagatgcgtc tgttgtggga
     2101 taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctatg cggcattacg
     2161 tgtgtccgtg caacgctggc aacttgatct ggctgtgcag ttgcatcccg caaggatgca
     2221 actgcacaac cgacccttga agaactggca gcgaagctgt ccggaggagt gaatggttac
     2281 agtcgctgaa ctgcaggcgc tgcgtcaggc gcgccttgat ttattaaccg gtaaacgggt
     2341 tgtgtcggta cagaaagacg gtcgcaggat cgaatatacg gcagcgtccc tgaatgagct
     2401 taaccgtgcg atcaacgatg cggagtcggt tctgggtacg acccgacgcc gccgaccgct
     2461 gggagtgagg ttatgaaacg aacgcctgtc ctgattgatg tgaacggcgt tccgcttcgt
     2521 gagagtctca gctacaacgg aggcggcgca ggatttggcg ggcaaatggc agagtggtta
     2581 ccaccggcgc agagtgccga tgcggccctg ttgcctgcat tgcgtctggg gaatgcccgg
     2641 gcagatgatc tggtgcgcaa taacggtatc gcggctaatg cggtggccct gcataaggat
     2701 catatcgtcg ggcacatgtt tcttatcagc taccgtctga actggcgttg gctggggatg
     2761 cgggagacca cggcaaaaag ttttgtcggg gaggtggaag cagcctggtc ggaatacgca
     2821 gaaggaatgt ttggcgagat cgacgtggaa gggaaacgca cgtttacgga atttatccgt
     2881 gaaggggtgg gcgtgcatgc ctttaacggc gaaatctttg tgcagccggt ctgggatacg
     2941 gaaaccacgc agttattccg tacgcgtttt aaagccgtga gtccgaaacg ggtggacacg
     3001 ccaggacacg gtatggggaa ccactttctg cgggccgggg ttgaggtcga tcggtatggg
     3061 cgggcgattg cgtaccatat ctgtgaggat gattttccgt tctctggtgc cggacgctgg
     3121 gagcggatcc cgcgtgagct tcccaccggg cgtccggcca tgctgcatat ttttgagccg
     3181 gtggaggacg ggcagacccg tggggctaat cagttttaca gcgtaatgga gcggatgaag
     3241 atgctggact ccctgcaggc cacgcagctt cagtcggcca ttgtgaaggc gatgtatgca
     3301 gccacaatcg aaagtgaact tgataccgaa aaggcatttg aatatatcgc gggggcaccg
     3361 caaggtcagc aggataatcc gcttattaat attctggaga agttcacccg ctggtatgac
     3421 acgaataacg tgacgctggg cggtgttaaa attccgcacc ttttttcctg gcgatgcgct
     3481 gaacctgcag acagcgcagg attcagacaa tggattttcg gcgcttgaac aggcgctgct
     3541 gcggtatatt gcagccggtc ttggcgtttc ctacgaacag ttgtcccgtg attactcgaa
     3601 ggtcagttac tcaagtgccc gcgcctccgc caatgagtcg tggcgctatt ttatggggcg
     3661 gcgaaaattt attgcggccc ggctggccac gcagatgttt tcctgctggc tggaagaggc
     3721 gcttcttcgg gggattattc gtccgccacg ggcgcgtttt gatttttacc aggcgcgttc
     3781 agcctggtca cgggcagagt ggattagctc cggaagaatg gccattgacg ggctcaagga
     3841 ggtccaggaa tcggtgatgc gcattgaggc cggactgagc acgtatgaga aagaactggc
     3901 actgatgggc gaagattatc aggacatttt ccgccagcag gtcagggaat ccgcagagcg
     3961 cgaaaaagcc ggactctcac gtccggtgtg gatagcccag gcgtatcagc agcagatagc
     4021 ggagagtcgc aggccggaag aggagacaac atcacgtgag acgtaatctt tcacatatta
     4081 tcgctgcggc attcaatgag ccgctgcttc tggagcccgc ctatgcgcgg gttttctttt
     4141 gcgcgctcgg gcgtgagatg ggcgcagcaa gcctttcggt accgcaacag caggtacagc
     4201 ttgacgctcc cgggatgctg gctgaaacgg acgagtacat ggccggaggc aaacgacctg
     4261 cccgtgttta ccgggtggtg aacggtattg ctgtactgcc ggtgaccggc acgctggtac
     4321 accggctggg tggcatgcgg ccattttccg gaatgactgg ctatgacggc attgtcgcct
     4381 gtcttcagca ggcaatggcg gacactcagg tacggggcgt actgctggac attgacagtt
     4441 ctggcgggca ggccaccggc gcgtttgact gcgctgacat gatttaccgt ctccggcagc
     4501 tgaagcctgt ctgggcactg tgcaatgaca cggcctgttc agccgccatg ttactggcat
     4561 cggcctgctc ccgacggctg gttacccaga catcccgtat cggctccatt ggggtgatga
     4621 tgggccatgt cagctatgcc ggtcatctgg cacaggccgg tgtggatatc acgctgattt
     4681 acgccggatc gcacaaggtg gatggcaatc agtttgaatc cctgccagca aaggttcgcc
     4741 aggacatgca gcagcgcatt gatgcggcgc gccggatgtt tgctgaaaaa gtggcgatgt
     4801 ataccggttt atctgtggat gcggttatgg gaacagaggc cgccgttttt gaaggtcagt
     4861 ccggtattga ggccgaactg gcggatgaat taatcaatgc gtcggacgcc atcagcgtga
     4921 tggctgcgac gctgaacaca cacgatacag gaggcactat gccgcaatta actgcaacgg
     4981 aggctgccgc gcaggagaac cagcgagtga tggggatcct gacgtgtcag gaagcgaaag
     5041 gacgtgaaca actggccacg atgctggcag ggcaacaggg tatgagcgtt gagcaggccc
     5101 gggcgattct ggccgtggcg gcaccgcaac aatctggtgt atctgcgcag agtgaagccg
     5161 atcgcattat ggcatgtgaa gaggcgaaag gacgtgaaca actggcggca acgctggcgg
     5221 cgatgccgga gatgacggtg gaaaaagccc gtccgattct ggctgcttca ccgcaggcga
     5281 atgccggacc ctcacttcgt gatcagatta tggccctgga tgaggcaaaa ggggcagagg
     5341 cgcaggctga aaaactggcg gcctgtccgg ggatgaccgt ggagaatgcc cgggctgtac
     5401 tggcggcagc ctcaggcagg gcagaaccgg tttctgcatc cacaaccgcc atgtttgaac
     5461 atttcatggc gaaccattca ccggcagcgg tacagggtgg cgtgccacag acgtcagcag
     5521 acggtgatgc ggacgtgaaa atgctcatgg ccatgccatg aagtcagtgc tgaccatcaa
     5581 tatgaggttt ttacaatatg gtaacgaaaa ccatcactga acagcgtgcg gaagtacgta
     5641 tttttgccgg taatgatccg gcccataccg ccacaggctg cagcgggatt tcctcggcaa
     5701 caccggctct gacgcccctg atgctggatg aggataccgg gaaactggtg gtctgggacg
     5761 gacagaaggc aggccatgtg gttggcatac tggtactgcc gcttgaaggt acagagacgg
     5821 tgctgacgta ttacaagtcg gggacctttg cgacggaggc catccactgg cctgagagag
     5881 tggatacaca caaaaaggcg aatgcctttg ccggcagtgc cctgagtcac gcagcgctgc
     5941 cgtaacattt tatcaggtca ctgcggtagc ctgactgatt tctgaatgaa aggaactgat
     6001 ttatgggatt gtttacgacc cgccagttac tcggttatac cgaacaaaaa gttaaatttc
     6061 gtgcgctgtt tctggagctg tttttccgcc gtacggtgaa tttccatacc gaagaggtaa
     6121 tgctggacaa aattaccgga aaaacgccgg tggcggccta tgtctccccg gttgttgaag
     6181 gaaaggtgtt gcgtcaccgt ggcggtgaaa ctcgtgtact gcgcccgggc tacgttaagc
     6241 cgaaacacga atttaattac cagcaggcgg tggagcgtct tcccggtgaa gatccggccc
     6301 agctgaacgg cccggcttac cgtcgcctgc gtatcatcac cgataacctc aaacaggaag
     6361 agcacgccat tgtccaggtg gaagagatgc aggcggtgaa tgccgtgctg tatggcaaat
     6421 acaccatgga aggagagcag tttgaaactg ttgaggtcga ttttggccgg tcggtgggaa
     6481 ataacattac gcaggcagga gggacggagt ggagcgctca ggaccgcgat acctttgatc
     6541 cgacgcatga tctggatgcg tactgtgatt ttgcttccgg caccatcaat attgccatta
     6601 tggatggtaa ggtctggcgt ctgctgaatg gctttaagct gttccgcgaa aaactggata
     6661 cccgtcgtgg ctcaaattca cagctggaaa cggcagtgaa agacctgggg gcggtggtgt
     6721 ccttcaaagg gtattatggt gatctggcaa tcgtggtggc aaaaacgtcc tatgtggcag
     6781 aggacggtac cgaaaaacgt tatctgccgg acggcacact ggtactggga aatacggccg
     6841 cagagggtat tcgttgctac ggtgccattc aggatgcgca ggcattgtct gaaggcgtgg
     6901 tggcgtcttc ccgttatccg aaacactggc tgaccgtggg tgatccggcg cgtgaattca
     6961 ccatgacgca gtccgcgccg ctgatggtgc tgccggatcc ggatgagttt gtggtggtgc
     7021 aggtgaaata atccgtgagc gggagctaaa tgctcccgtt tttcattcaa tcaggagcgg
     7081 gtatggctac gaaagaagaa aacctgaaac gtctttgtga gctggctgag aggctggggc
     7141 gtgaaccgga tgtctccggt agtgcggcag atattgcgca gcgagtggcg gaactggagg
     7201 aagagcttgg agatgcgggg gaggtcgcag agccggatac gtactcacca tcgaaagatg
     7261 atccgaccgc gcataaaggc gagtcagttc ctgaacagcc aggcattgtt accggagacg
     7321 aaacggggca ggttacggtg gtggcactgg cgacactgca tacaaaatgt aatggttcag
     7381 tgatatttgt ttcaccggga acgtcgtttc gtgtttctgc tgaggtggct gccagcatgg
     7441 cggcaggtgg tctggcaaaa aggcagtaac aggagatgtc gtggacggtt tcgataatct
     7501 gtttgatgct gcgcttgccg gtgtcaatga agtcattctc cgtgatatgg ggatcagcgc
     7561 cgtcattacg tccggagaac ttgaggggac acacctgaca ggggtttttg atgatcccga
     7621 aagtatttct tttgttgccg gggggatccg gcttgaggat tcctcaccgt gcctgtttgt
     7681 gaaaacggca gatatatcgc agttacgccg tcaggatacg ttaactatcg gggatgattc
     7741 tttctttgtg gatcgtatta ctccggatga cgggggatgc tgttatatcc ggttaaggcg
     7801 gggcagtcct gccccacaga ataatgccag aatgaggcga tatgatgaag ggacttgaga
     7861 atgccatccg caatctgaac agcctcgata cccgcatggt gccacagacc agcgcatggg
     7921 cggtaaaccg tgtggcggca aagattgttt ctgtcgccac acggcaggtt gcgcagaata
     7981 ccgttgccgg ggataaccag gtgaaaggta ttcccctgaa aacggtcagg gagcgtgtca
     8041 ggctgcttaa agccagcccc tcaggaaaaa tgtatgcccg catgcgtgtt aaccggggta
     8101 acctgcccgc cattaagctg ggtaccgcac aggtcagact ggcccgctcc cggcatggca
     8161 gcaactcacg tcatcgtggc agcgtactga aagtggggaa ataccttttc cgggatgcgt
     8221 ttattcagca actggcgaat ggtcgctggc atgtgatgcg gcgtattgac ggcaaaaatc
     8281 gttaccccat tgatgtggtg aaaatcccg
//
LOCUS       DAZXUL010000165         8280 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZXUL010000165 DAZXUL010000000
VERSION     DAZXUL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.165"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            8..658
                     /locus_tag="ACH3L1_003839"
     CDS             8..658
                     /locus_tag="ACH3L1_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078388.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            630..1064
                     /locus_tag="ACH3L1_003840"
     CDS             630..1064
                     /locus_tag="ACH3L1_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFT4078389.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1837..2886)
                     /locus_tag="ACH3L1_003841"
     CDS             complement(1837..2886)
                     /locus_tag="ACH3L1_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFT4078390.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3337..4527
                     /locus_tag="ACH3L1_003842"
     CDS             3337..4527
                     /locus_tag="ACH3L1_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HFT4078391.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4917..6377)
                     /gene="gtrII"
                     /locus_tag="ACH3L1_003843"
     CDS             complement(4917..6377)
                     /gene="gtrII"
                     /locus_tag="ACH3L1_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HFT4078392.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGASTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6374..7303)
                     /locus_tag="ACH3L1_003844"
     CDS             complement(6374..7303)
                     /locus_tag="ACH3L1_003844"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFT4078393.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7300..7662)
                     /locus_tag="ACH3L1_003845"
     CDS             complement(7300..7662)
                     /locus_tag="ACH3L1_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFT4078394.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(7924..>8280)
                     /locus_tag="ACH3L1_003846"
     CDS             complement(7924..>8280)
                     /locus_tag="ACH3L1_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFT4078395.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
BASE COUNT         2530 a         1597 c         1687 g         2466 t
ORIGIN      
        1 gtaatccatg catcgtatag acacgaaaac cgcgaagaag gataagttcg gcgcgggtaa
       61 gaacggtttt acccgtggta acccccagac tggcacgcct gccaccgatc tggatgatga
      121 ctactttgac atgttgcagg aggagctttg cagcgtggtg gaggcatccg gtgccagcct
      181 ggagaagggg cggcacgatc agttgcttac cgcacttcgc gcgctgctgt taagccgcaa
      241 gaatccgttt ggcgatatca aatcggatgg cacggtgaaa acggctctcg aaaaccttgg
      301 tttgggagaa gcagctaaaa ggaatgtagg tacaggggcg aatcagatac ctgatatgag
      361 cctgttcgcg tcaattaata ccgtaacggc tgctgcgcaa aaatttccgt ctggattaat
      421 tttacagtgc ggtcagttga atggtgcccc gaatgtatct tcaacatacg ggatgaggtt
      481 cccgatgacg ttctcaagag taattgctgt cgtagttaca ttgaacgtta ctggcgcggc
      541 tgggcagccg actgtatcgg cgacaagtgt ccagaacact ggatttaata ttactgtgtc
      601 gcccggttca ggatacggtt catctgctga tgcgtattac attgcaatgg gatattaaca
      661 aaatgtcata tttttattct gcatcgacaa acggatttta ttcgactgaa tttcacggca
      721 ccaatattcc tgatgatgca gtggaaatct cggaatcaga gtggaaaaca ctgattaatg
      781 cacagagtgt aacaaaaatg attacctgtg gtgagaacgg tcatcctgtc attgttgacc
      841 gtccttctcc aacaccagaa caattagcct taataaatga tgaaaagaaa tctgcactga
      901 tagcagaggc aacgaatgta atagctccgc ttcaggatgc ggttgattta ggtatggcaa
      961 cagatgatga aacgaaactg ttactggcat ggaaaaaata tcgggtgctg cttatgcgtg
     1021 ttaatgtagt aaaacccgag tggcctatgc atccaaataa atagacttgt taatctagga
     1081 taattgttgg aataatactc gtttttcagt aggttcaggt agcctgggta taatgctcac
     1141 ttctacaatg atttcaactt attttattta attgatatta cgaaacttaa ctcatacata
     1201 aacaatatga ctcaatactg tcctaaatag ctgcgcagaa aagtgctcca ctttcgctca
     1261 gcccttgctg ggcgaagcat caggaaggcg ttctcagcta ggttgtgtta tatctggctt
     1321 gagttttgtt gcccatacgc aacacattac ccatctgtaa acgacaagtg tggcagaatt
     1381 aggtgcagct atttagacta tatcgacgca gtaacgataa tagatatcga aactgtacta
     1441 gatattagct acttatggtt accgggggga gtcttttgag cactggtgtt atcaattggc
     1501 ttcaaataat tcgccaataa attatattgc gatagctcat caccttccag gttgtttata
     1561 gccagtttat cagcgtcacc acctactgtt tatgctttta tttctttgat taataagtat
     1621 atgatatttt aattcatctg gtgtatttct ttctcatgtg ttggtcgact gaatatctgt
     1681 tcagtgaagg ggtaatttta tgtttgcact tcgaacattc ccttcagttg ctaggaaaga
     1741 aaaccgtaac atttgcgcat taattttaac agtataatct gagcataaat atgcgctata
     1801 tatctataaa aaggcgcaat taattgcgcc tttttatcat tttatcaaaa atggcccacc
     1861 gactgtcctc gatagccaat ggccaatttt atctgataca tatacacaaa tacaggtata
     1921 tatgattgcg cagataggat ttggaatagt cacgctgaag cctataattg ctaaagccat
     1981 agggataaat aatttggtga tatactccat tccacatatg ttaagagtat ttcttccagc
     2041 tcttacaata aagtcatttc cacaaattat ttttgctatg aaaatattaa agattatcaa
     2101 gcctaccgtg gtgataatag tattaatagc ttgaatataa ctacttgata ttatgtctgc
     2161 attgaatatt gagataatct taaaaagtaa aggtttctgg ttaagtagtt gatacgcact
     2221 cattatagcg gtaattgaga agacaatgaa accaatttta gattttccaa aaaacctgtc
     2281 acgtgttaat tctaaaaaca tacatctacc gagaggcaat aaccaccagt atgccatagc
     2341 ggagtctatg ttccaaaacc attgaggatc tagtaatggg ttatgattga gcactgtttg
     2401 tgacaacatg aacgataaca gtgatatgat taaaatcact atattgttct tgactatttc
     2461 tctaaagatt gcatcaattg ctattataac aaaaaggcag tttataaacc atattgttcc
     2521 tacgaattgg ttatttctaa caccatatat tatatcataa atgtggttgt atatataatc
     2581 aatcgtctca cctgagttaa ttgtgtttat tattaatata gaaaatgcaa aagtgaaata
     2641 tggaatcatt aatctgtaaa actttgactt gatataatcg aaaacagata agtcattttt
     2701 tttgatagta aaaaaaccag cagcgaaaaa gaatagtggg acgtgataac taaaaacaaa
     2761 aggatacaat ttgccagccc ccaacccaag gtgacctaaa tatatagcaa aaataccgag
     2821 aaattttaaa gcatcaaccc aatcatgtct tatttttctg ttgctgtcga ttttgttatt
     2881 atacatttaa agtaggctct ccagttttgt gtgattttta aacactcatt gcaaatatga
     2941 atgcctgaaa gtttatgatt attaatcaac gagttcaata ttaatttatt atttttcaaa
     3001 gtcgcactat ataggcattt acttccgcag taccaccgat agtatcgata tcaaatcagc
     3061 ctgcggccgc gataactttc gtaacattct gccagcggct ttgttgaata aatcaggttc
     3121 aagataatcg tctctgcatc aggttagagt ttcaggcaat acatcctctt tctggcatac
     3181 ctgccactct cgcaccatgg tcatagcctt tacgatctgc atactcacca gaccagagcc
     3241 cgctcccttt taggctgagg aatccccaga aaagaagaca ggcatagagt tttgtgatct
     3301 actgattgcg ctaacaaatt caatgagatc acctctatgc ctgcccgtaa agtatgccag
     3361 acttttttcc gtaatgcttt agcttccacg catcagtatc gacaaaatgc cattattgat
     3421 tctgctgctg ctttagtcgg tggcgcatct ctttctctta ccagcattgg ccggcattta
     3481 cccggtcctg ctcgcgtgaa agataaaata aaacgcgttg actgtctgtt gggtaataaa
     3541 cgacttcata atgatatccc tctgatattc aaaaatatta catccatgat gacaaataaa
     3601 ctgtcgtggt gtgttatcgc tgttgactgg agcggttacc cttttcagga atatcatgtc
     3661 cttcgtgcca gcctcctctg cgacggtcgc tctatccccc tgatgagtca ggtcttcccg
     3721 tcaaaaaaaa ataacgaggc ggtggaaatc gcttttctgg atgcgctttc cggggccatt
     3781 tctccccgga ctcgtgttgt tattgttact gatgctggtt ttcaaagtgc atggttccgt
     3841 catatcaaat cacagggatg ggattttatt ggtcgtatca ggggggtagt gaagttcagg
     3901 ctggatagtg acaaagataa gtggctggac ataaaaatgt gcagggggag ttcagaggca
     3961 aaatacctgg ggacgggaac cctggcccgg aaaaaacgct cgcagtgtga aggacatttt
     4021 tatctttata aacattcacc taaaggcaga aaaagcaggc gtgccagagg gaggccgggc
     4081 ttaccaacaa cggaaaaaga acaaaaagcc gcaggcagag aaccatggtt aatattcagc
     4141 aatacagctg agtttaatgc caaaaaaatc atgaaactgt acagtcgaag aatgcagata
     4201 gagcaaaact ttcgggatga aaaaagtgaa cgctttggtt ttggccttcg ggcgagcaga
     4261 agtcggaggg gagagcgttt tctggtatta agtctactgg ttacactggc cagtatcgta
     4321 ctctggctac ttggttatca ttttgaaaat aaaggatttc atttaaagta tcaggctaat
     4381 agtctcaaaa aacgaagagt attgtctttt ctgacgctgg ctgaaaacgt cctgagattt
     4441 aaccctgaac ttctacgacg agcacaacct gaaaagatat tgtgccggtt ggccagcact
     4501 taccgaagta tggtgctggc ttactgatga aaattttctg gggatccctc agcctttcgg
     4561 ggaggaatga gggctctgat attcttttgc cgtagttcat cgtgaaaccg tcggatgtca
     4621 tttggcctga gtgacccatg cctcccaaaa gcagagtcgc tagtgttatg taattaggcg
     4681 gttaaacttc aattctcagt gttaagtttg ggtaaacgct tgtaaatggt attgctaatt
     4741 ttttaattga ctaatattag aaacggttag tgtgattgta tatagatatt tgagtgtaag
     4801 gtatttaaag gggcattcag cccctccgtc tatttgtgat tgcatccatt gatgtgtgta
     4861 ttattttgag cttgaaaaag ggaggcgctc tttaataata aataaatcta tcaaagctaa
     4921 atattaaatg gaagccaccc ttttttctct gatagtttta ttatcaccaa accatttatt
     4981 tcagctatcg aatctggatt cggccaggaa ggtaaactct tcacaattgg ataaatcttc
     5041 tctatttgtt ctttatttgc tggtttgcaa ttacagattc catagtaatt cataaatgca
     5101 accatcctaa aatagttgcc accatcccac caaaaaaaag attttccaaa agtgtctgcg
     5161 ctcatggaga gcatatttcc tgagtcgttt gaaccatata tatatatata ttttccgtta
     5221 agggaaatgc cttttgtttg agatgtgtat gatatttctt tagctaaaga gatatctttc
     5281 tgtcttgcga gagtatcatt caaaaataga tttttggatg ttgaagcgcc attaaatata
     5341 ataaataaaa aaaacatgca gtttaggtat tttatagagc gaaaattgct taagcaagaa
     5401 aacaaaatta ctgctactat aggcattaaa acaaacaggc gaggtggcgc ccctgagcca
     5461 acaacaacga taaatataac cggaagtata taaatgaaaa tgattgatga aatcaaataa
     5521 attgatcttg tttttaattt atatgttagg gtaaacatca gaattaataa aggaatcagt
     5581 aaccacttgt ataggtttaa accagtggga ggattgttat agaagttata tatattatct
     5641 atggctgatt taacaagcca tttaatatcg cttgcatttt gtatataatt tgagatgtac
     5701 tcgttcgatt caagcgaaaa ataatgcttg atagctttgg ttaatagcag gtaaattaag
     5761 gtagataaag ctatgaggga tagagataaa catgaactat aaaaagattc acgaatattt
     5821 ttctcatttc gtattatcga atttatctgc aacccaatga cgaatgcaat aatatatgtt
     5881 acgaatgttt ggtagattgc cattgaaaga atggacagta ctataccaga aaatatcaca
     5941 atcctatttt tttgcgagtg aaaaataatt gctgatatcg ctgccagtag aaaagcaatt
     6001 cccacagtat cagcttggtt aagaaactct aattgatagg agatctgagg gaaggtaata
     6061 aatactaaca tacctatcaa taattcataa gattctagct ttagcgatct gcagattata
     6121 aatgctgaaa tgatgataaa agataaggct attaatggtg ttatataaag tgaaaaaggc
     6181 tcagggagaa agtaatgtcg caaaaaagtg tgaaaccacc gcccaagcgt gattgtttga
     6241 taaaaattat ttgtaaactc gccatcaata ttaagtgttc catcgtacaa ccacccccaa
     6301 taacaataaa ttgatattgc aaaacatgat atgaaggcaa ggagatttgc atttttaaat
     6361 aaattaattt taatcatggg ttacctcgat gagatttttt tatgatgtat ttgggtctat
     6421 tttttacttc aatatagatt ctacctatat attctccgag aacaccaatc ccgatcagtt
     6481 gcactccacc caagaaaagt attgatacaa gcagggaggg atacccgcgt actgggttac
     6541 caaagacaag ggtgtctata atcatccatg caccatataa aaatgaaatg cttgcaacaa
     6601 acaagcctat ataagtccat acgcgaagag gaaaggttga aaaacttgtg ataccttcca
     6661 gtgccagatt ccataatttc cagccattaa attttgagat gccagcaaca cgctctgcgc
     6721 gtacatattc aacgacatcc gtcggaccac ccacccagct cagtatgcct ttcatgaaaa
     6781 gattgcgctc aggcaacagt ttaatgttct ccacaacctc acgagacatg agtcgaaaat
     6841 ctccgacatt ttcctcgatc tttggggtgc ttattttgtt gtgtaattta tagaaccact
     6901 cagctgtctt acgtttcagt cgtccatcag ttgagcggtc tgaacgttta gcaagcacca
     6961 tgtcagcacc tgcctgccac ttttcaataa gatgagggat aacctcaatt gggtcttgca
     7021 ggtcgacatc aataggaatt acagcatcgc cgcttgcatg gtctaaccct gcaaataagg
     7081 ctggttcttt accaaagttg cgtgtaaatg acagcggaac aactagcggg tctgaaacag
     7141 ccagcgcgtt aataattgac tctgtggcat ctttacttcc gtcatttatg aatacaattt
     7201 ctacttcata tggcttcaac tcttggaatt cacgtaccgt tttatagaaa acaggtatcg
     7261 cttcttcttc attgaagaca ggaacgacaa gagatatttt catttcgcat ccctaaagac
     7321 aatgaacttt gaatagacga aaccgcacac cagactgatg gcggagaagg tgataagagt
     7381 gactattgga ggaagtgagc atttatcagc tgcccaacca acaatcacac tcaatattcc
     7441 cataaatccc acgtataaca tgtagcgcat cgctgtagtc aatgctttga atgtgaatct
     7501 tgcattcgcg aagaagctaa agctcacagc cactacgaaa cctgtgaagt ttgccagagc
     7561 ctgactggta tgtgcggcat agatacatac accaaaaacc acccagtgta taagtgtgtt
     7621 cagcacacct atagatgtgt actttacaaa taactttaac atttatttaa tcaatgagct
     7681 ctgaaaggca tgaagtctat catccaagtc tcaattgatc gatacttgct atgtctgatg
     7741 agacaaaact gagacacata aggcctcaca atggcttgca aggctttaca tgttttgatg
     7801 tggtgggacg tgtgagcgca gtgttgatgg gataatcctt tgaattacaa gcggattctt
     7861 ataattcgta atgcgaaggt cgtaggttcg actcctatta tcggcaccat ttaaatcaat
     7921 aagttacctc gcatttaagt aaactacgtt ctcctcttgt gccgtatttg tgccattgcg
     7981 acttataatc gcatcgattt tgctcgcgtg ctcggtaaga tgcccggctg aaaggtgggc
     8041 gtatctttga accatttcga gagtttccca tcctcccatc tctttaagcg caagaagaga
     8101 gacaccggac tgaaccagcc agcttgccca ggtatgcctc aggtcatgga agcggaagtt
     8161 gctaatgcct gcccgcttta acgctccctt ccatgccttg ttgctgtcgg ttctcatctt
     8221 ccttaccgct gctgtttttg ttccgtcgct tcggtaggca ggtttggtgt ggacaaacac
//
LOCUS       DAZXUL010000166         8110 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZXUL010000166 DAZXUL010000000
VERSION     DAZXUL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.166"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..932
                     /locus_tag="ACH3L1_003847"
     CDS             <1..932
                     /locus_tag="ACH3L1_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFT4078396.1"
                     /translation="KVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIKDEDLS
                     NSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGTLNACS
                     AMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSFSKLDL
                     INFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNFNNANL
                     SNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFF
                     EEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(1086..2735)
                     /locus_tag="ACH3L1_003848"
     CDS             complement(1086..2735)
                     /locus_tag="ACH3L1_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFT4078397.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2886..4235)
                     /gene="ghxP"
                     /locus_tag="ACH3L1_003849"
                     /gene_synonym="yjcD"
     CDS             complement(2886..4235)
                     /gene="ghxP"
                     /locus_tag="ACH3L1_003849"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFT4078398.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4941..5405)
                     /gene="soxR"
                     /locus_tag="ACH3L1_003850"
     CDS             complement(4941..5405)
                     /gene="soxR"
                     /locus_tag="ACH3L1_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFT4078399.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5491..5814
                     /gene="soxS"
                     /locus_tag="ACH3L1_003851"
     CDS             5491..5814
                     /gene="soxS"
                     /locus_tag="ACH3L1_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFT4078400.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5817..7394)
                     /gene="pdeC"
                     /locus_tag="ACH3L1_003852"
                     /pseudo
     CDS             complement(5817..7394)
                     /gene="pdeC"
                     /locus_tag="ACH3L1_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            7818..8099
                     /locus_tag="ACH3L1_003853"
     CDS             7818..8099
                     /locus_tag="ACH3L1_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFT4078401.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         2249 a         2083 c         1855 g         1923 t
ORIGIN      
        1 ttaaggtggt atattttata aataatgtcg ccgtccataa taatactatc gaaattccac
       61 agatagtaaa tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag
      121 atgaagattt atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg
      181 actgtaatat gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata
      241 attgtattct cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg
      301 catgctccgc tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa
      361 ataccttcct tgactattca aatttttata tggcgtacat ggctgaggta aatctttata
      421 aagtaatagc gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt
      481 taattaactt tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga
      541 atataaactt aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa
      601 tggttatatg taccggctct aatatggcta atgttaattt taataatgcc aatttaagca
      661 actgccattt caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc
      721 gggttaattt cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg
      781 aaaatatccc cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt
      841 gtacctctca tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga
      901 ttactgcaga tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt
      961 gactttacaa gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc
     1021 tgttaaaggt gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt
     1081 tcggcttact gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat
     1141 ttttgcagcg tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt
     1201 tcagaacgca atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt
     1261 tcaacgtcgt tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc
     1321 tccgtaagca actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt
     1381 ttctggatcg ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc
     1441 aattgttgcg aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg
     1501 ccgacaaaca gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc
     1561 gggaagacgt taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc
     1621 gccccacgca ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac
     1681 tccatcggct tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg
     1741 aaacggacca gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt
     1801 tcgacgttag ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc
     1861 agacctaaca gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg
     1921 tttgcgcgca ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc
     1981 atcccggcgg caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa
     2041 gaagcgaatg gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag
     2101 cggctgagga atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata
     2161 ccgccaatgg ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc
     2221 cccatcgcca ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac
     2281 gcttcgccct gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg
     2341 gagagcgcca cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc
     2401 ggaatgcccg gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc
     2461 agcgccagcg cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt
     2521 ttccagccat cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca
     2581 aactccacat gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata
     2641 agcggaagcg ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg
     2701 agggtcatta tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt
     2761 atgatctgag aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca
     2821 cacaatgtgc ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg
     2881 aaagattaga tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc
     2941 gtaccgatgt tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg
     3001 aagccgatca tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc
     3061 agacccgcca tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc
     3121 agcaggccaa cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc
     3181 ggagagagga acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt
     3241 ttaccgcccg ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc
     3301 accaggccag agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg
     3361 atctgcccat ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg
     3421 gcatcaaata ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc
     3481 gcgcccataa tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc
     3541 atggcgaaaa cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata
     3601 ccgataatgg tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc
     3661 gccagacctg ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc
     3721 agaccatcaa gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg
     3781 aacagaccga taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc
     3841 cagctacgga tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca
     3901 cccagcgcga caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc
     3961 gtcagggaga tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata
     4021 gaaccgagtc cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct
     4081 ttacccaaca tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac
     4141 ccggcaacca cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg
     4201 agtgaaccgc cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa
     4261 ttttcgtcaa gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca
     4321 ggtttgcggc aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac
     4381 gggtggtaaa aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt
     4441 tgctgctttt ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac
     4501 gttatcgtcg atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat
     4561 aaaaaatcgt ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga
     4621 agaaacgtcg gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct
     4681 cgaatgaggt gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa
     4741 gacaaagacc ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga
     4801 taattccaaa agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg
     4861 gaacaggcag caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt
     4921 cagcttgtcc ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct
     4981 tcttctccta agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca
     5041 caaccaatac atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc
     5101 aactcttctc gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct
     5161 tctggcaaca cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg
     5221 attttgataa ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg
     5281 ttacggatac tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca
     5341 ccgctgcgtt tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt
     5401 tccataaatc gctttacctc aagttaactt gaggaattat actccccaac agatgaatta
     5461 acgaactgaa cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt
     5521 atcgcatgga ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa
     5581 tcaggctatt caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt
     5641 ggcgattaca ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag
     5701 cgtccgattt ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc
     5761 gttttccgtc ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat
     5821 tgcccgcgcg ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc
     5881 ggcatcgcct tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag
     5941 ttaacctgct cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc
     6001 agctcgatga tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca
     6061 aacgatttat cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag
     6121 tagccaatac caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt
     6181 aatcggcgtc attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg
     6241 ctgcggacgc accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt
     6301 atgaaaatcg gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta
     6361 cgcgcccaga tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc
     6421 ctccttttct gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg
     6481 ccaacgtaac aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg
     6541 gtaataaaga caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa
     6601 atagttttgt cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc
     6661 gggcaacgca aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc
     6721 aacaatggct gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct
     6781 ttgtacggtt aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt
     6841 gcttaacgag aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc
     6901 atcagacatc acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg
     6961 catataggtc attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac
     7021 gttaggttca cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag
     7081 cgatgagcat aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata
     7141 ttcaacattc gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca
     7201 tcgctcacta aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca
     7261 aaggttcgga gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa
     7321 agaatgatgg gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt
     7381 gcacgatgac tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa
     7441 accctggcta ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt
     7501 tgaaattgat acctttttaa tcgtcacctg gatgaaaata attcattgaa aaattaattt
     7561 tctacaggaa tacagaggcg gcgggaagat atgcccttaa tgccactgaa aaaccacata
     7621 atttgtacag caataaaagt cacggcctaa tatggctaac aaacaatcaa ttgtgatata
     7681 gttcacaaaa ttaatgaaac aaacagagtg tttcattttt gtgttctgca agccccgcga
     7741 tagccagagt gtaccacgcc tcccgtgaac aacgccgcgc tgtccagggc atcggctctt
     7801 ttcagaggaa ttgtttgatg gctaccctca ccactggcgt ggttcttctt cgctggcaac
     7861 ttcttagtgc cgtaatgatg tttctggcca gcacactcaa catccgtttt cgtcggtctg
     7921 attatgtcgg gcttgcggtg atcagcagcg gtctgggcgt tgtttccgcc tgttggttcg
     7981 caatggggtt gcttggcatc acaatggcgg acatcaccgc tatctggcac aacatcgagt
     8041 cggtgatgat agaagagatg aatcagacac cgccacaatg gccaatgatt ttgacctgat
     8101 acgtagaagc
//
LOCUS       DAZXUL010000167         8070 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZXUL010000167 DAZXUL010000000
VERSION     DAZXUL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.167"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..92
                     /locus_tag="ACH3L1_003854"
     CDS             <1..92
                     /locus_tag="ACH3L1_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078402.1"
                     /translation="GAVQRAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(106..297)
                     /locus_tag="ACH3L1_003855"
                     /pseudo
     CDS             complement(106..297)
                     /locus_tag="ACH3L1_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            295..619
                     /locus_tag="ACH3L1_003856"
                     /pseudo
     CDS             295..619
                     /locus_tag="ACH3L1_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(649..3561)
                     /locus_tag="ACH3L1_003857"
     CDS             complement(649..3561)
                     /locus_tag="ACH3L1_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFT4078403.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            4044..4886
                     /locus_tag="ACH3L1_003858"
     CDS             4044..4886
                     /locus_tag="ACH3L1_003858"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFT4078404.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FCYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4889..5977
                     /gene="capU"
                     /locus_tag="ACH3L1_003859"
     CDS             4889..5977
                     /gene="capU"
                     /locus_tag="ACH3L1_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFT4078405.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5982..6932
                     /gene="virK"
                     /locus_tag="ACH3L1_003860"
     CDS             5982..6932
                     /gene="virK"
                     /locus_tag="ACH3L1_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFT4078406.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6997..7941
                     /gene="lpxM"
                     /locus_tag="ACH3L1_003861"
                     /gene_synonym="msbB"
     CDS             6997..7941
                     /gene="lpxM"
                     /locus_tag="ACH3L1_003861"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFT4078407.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2169 a         1810 c         1926 g         2165 t
ORIGIN      
        1 ccggggcggt tcaaagggcc ttcccgagag tggtaaagga gaggccctgg aaatacccca
       61 cagccccgaa aaagagccag tcagttgctt aactgactgg cattaaggcc gccggggacg
      121 tttttttacc accgggagtt tgggcataac ggtgctttca gtaagtacga caggtactgt
      181 ggaggcactt ccgtaaagaa gggcatcaag ctcctgctcc gacatacctg caggcaaagg
      241 ccatgaaagc cctgatttcc ggaagcgagc gaacagtcca caaactgttg aacgtggtgt
      301 cagcggcgga cagggataag ccctcgtcag cagttccgtc agcactgcga cagcgttctt
      361 tacccggtca aaacagcgtt gcggtgcacc acgcctgacg gatgaactgc gtgctcaggg
      421 ttaccacttt aacgtaaaaa ccgtggcggc aagcctgcgc cgtcaggggc tgagggcaaa
      481 ggcctcccgg aagttcagcc cggtcagtta ccgcgcacat ggcctgcccg tgtcagaaaa
      541 tctgctggag caggattttt acgccagtgg tccgaaccag aagtgggcgg gagacatcac
      601 gtacttacgt acagatgaag ttcggttaca tcctgtcagc acggaacctc acgctttttg
      661 agcggcacga cggatccccc ccacccagta tccgacagac ttttcactgc gttcgtaaaa
      721 acccttcttc cctcccagca tatccactaa ttccggtgac atatcctgca gataagtctg
      781 caaatcatgc cccagatctt ccggtgactg caactcataa accgcgcgcc acacatcacc
      841 cttccttatt ttaagccccg ggttaagaat atactgtccg tggttcagcc tcagaaaaag
      901 cttttttcca taaggatttt tgctgttaac ttcatgttga gagagctggc tgctgatata
      961 actccttcgt ttgcggcgtt ccggcagcaa cgcttcagga agatgaacca cacactctgc
     1021 cagtcgtgcc gcactgaagg cttccgaaaa aaacagagca cttcccagat gcgtgtggaa
     1081 cagcgcctgc atcaggatca ccagaaacat tgccatctgc cggttgtcca gtttgatgag
     1141 gcgtccttcc acctgcagtg acagactttc aggtttcagc agggtataca actgcgcact
     1201 tttctgttgc gcataacggg gcgtactgac cgccgctgac agaacttgat gataagccgc
     1261 cagcccgtca ttaccggtca gcaagggatc tgcaccacgc tccagcagta actgaaccag
     1321 tgcaatatta cctgcccggg ctgctgacat cagcggagta agattaaacg gcgaacggtg
     1381 atcaacacca taacgttcaa tatcccgcaa cacggcaacc gggttattca ttgagtatgg
     1441 ccggaaatac ttcagctcca gtaactgcag agtcttcgtt ctgggctgcc ttgtgggctt
     1501 aaaatcttcc cggtacagtg cattatacag cgggaacagg gaataaatca gactgtactc
     1561 cagcgcctgc agttgtatgg ttttatcggc tgtacctgcg aggatatgct gtttccactg
     1621 tcgtgcatcc tctgcggtga taatctgcca tggcatctca gatgtctgca ggatccggct
     1681 acggatggtg tccgcctgct cctgcttccc ctgtttttca agcagatgtg cggttttctg
     1741 ccactcatca cggctggaaa tctcttcctg cagattcaga ggctcttcct gatgcgtgag
     1801 cgcaagcaga ctccacagag gatgctccac ctgctgttca acaagataca cattgtgttt
     1861 agcacgggtg agcgcaacat acagcgcgtt ggtaaaaaat ttatagattt ccgctgaacg
     1921 atcctgcctg tcccgcggac gggaataacg ggcctcgccc tcaagatcag ccggtgtaag
     1981 accctcgcaa atatcatcaa atgcctgccg ggcagcgctt actatattgt aaaggatcac
     2041 cgtttcgtat tccagcccct taacctcctg aaccgaaaag accagcggcg tactgaacca
     2101 gcatcgcgcc tgtgctttct gttccggatg catcacaatg accgctacac gattcgaaag
     2161 tgaggttttg gcattcagtt cctgctttgt ctcttcccta tcgtccagca accggatggt
     2221 cccttctgcc tgaccgcagc ttctgacaaa atggtgactt tcccgatcaa tagcactaaa
     2281 gcggacctgt ttgagtctca gcaaccggtt agccagtgca gtgacgtggt gcccgttacg
     2341 gtaattcgcc tgcaggatat taacggttgt gtcgtttccc tgttgctgcc ggaaaaagag
     2401 acttttgagc gaactccatg agaaaaagct cggatgaact atctgattgg catcaccaca
     2461 gagtaaaaac tgaccgggat gacgtagcgt tttcagtacc agttgcagtt gaacaggcgt
     2521 catatcctga acttcatcaa caaaaatgac atcataacgg ggagttgcca acgaaagata
     2581 ctgatgactg agcagattac tgtcatactg atgcgactgt ttcagccatg cgatataacg
     2641 ttcaaacagg acatagaccg tcgggcggtc ctccatgcca tagagcgact gccgtatccc
     2701 cagtgacaga tacgcctccc gggacagcgg cccattcccg gaggcaaccg caccgatcac
     2761 gcccctgaac tcctcataca gggtatgggc agcccccagt gcggcgatag ccctgttgcg
     2821 gggcagccag tcgctgaacg cactgaaagt cacctccctg ccttccggaa tgcgcagagt
     2881 ttccagaaat tccgtcagcg aaagaaagtc aatattctgt acctcactcc cttcccctga
     2941 agcgtcatag agagtacgcg ctttttccac cagaaatgag gaaaggctga gatacagtat
     3001 atcaccagca gcctgcttca ttttttccag taccagcgcc gtcttaccac ttcccgccga
     3061 gccaacaatc accagcggta gcgggtgttc gtacagtgcc tgctgatcat catcaaagga
     3121 cagcatttta tccaggcggt ggaaacgccc atgagacgga ttgatatacg tcagcgcttc
     3181 agtcgcgata tcaacaggat ccggcactgg ttgttgttgt aagcgccctt catcaatcac
     3241 cacgtttctg cgaagaaaac gtgaagtgtt ataggcatga ttgcgaatat attccagaac
     3301 aaggcagata gtttcaccgt ggtagcgata cagcgaaaac agtaatcggt cccggatact
     3361 gagccgggcg cggtaaagat tggtgtcaat tttacgaaca tcagcctgaa caaagttatc
     3421 ggtggcaata gcctgtgcaa attttttata acccgggatc gtgtcaggat tcagagtatg
     3481 ataaagacag attttcatgg ctgccggttt ccgtcgtgca aaaaacatgc tgattttacg
     3541 aaggaatgcc ttactgaaca accttgcccg gaataccccc cgaaatcaga tggaacaatc
     3601 agaaaaatgc tctgcagtta tggaataaag aaggtggggt ggtacgccgg gagggcgatc
     3661 cgctggaaat ggcgcagtag ctgcaggcct gtcatgatgc aggaatagaa gttcgtgtac
     3721 aggttaatga aagcgtcacc ccgtaaataa actctgcgct gaaattcctt tttttcattg
     3781 gagtacctgt gttgttctga ggtgaacata tcacctctgt tcaggtagcc aaattcagtg
     3841 tgccacctca aggttcttag tgagtcgttt ttaaaagata tgataatata tcattgttta
     3901 tgaattgttg atgtttaatt tagtaaatta tatcaaatat gccatgggtt tgctatgtta
     3961 ttcatccagt atctggaaat aaaatgcacc agctttattg ggttttcctt ttgtctctgc
     4021 ttgctaggtg ccagaaactt taaatgttaa acgaaggggg cattttgttc aaagccaatc
     4081 atgttccggt attaatgtat catcatgtca gtcattgccc cggactggtt acactttctc
     4141 ccgtgacatt ccggaagcaa ataaaatggt tggctgaaaa caactggaaa acactgtcat
     4201 cagatgaact ggagtttttt tatcggggcg gaaaactccc ccggaaaagc gttatgttga
     4261 catttgatga tggatacctt gacaactggt ttcaggtata tcctttatta aaagagttta
     4321 atctcaaagc acatattttt ttaattaccg gttttattgg gaatggtccg gttcgtcact
     4381 ctcctggaaa ggaatattct catcgcgatt gcgaacatca aatagcgaca gggaatgctg
     4441 ataatgttat gcttcgctgg tctgaagtaa acgaaatgtt gcaatctggt ctggtcgaat
     4501 ttcatgttca tacgcatact catacacgct gggataaaaa gttcagttca cgagaagaac
     4561 agtgtaaaca tcttcggcag gacttgttga gcgggaggga atatttgaaa gaaatgacag
     4621 gaaaatgcag taaacatttg tgttggccgg aaggttatta taacaaggat tatattcagg
     4681 tcgctgaaga acttggattc tgttatttat atacaacaga aagaagaatg aatgctcctg
     4741 ctaaaggaac tacacggata ggaagaataa gcaccaaaga acgggaaagt tgcgcatggc
     4801 tgaaaagacg acttttttat tatacaaccc cgtttttttc atcgttgctg gcctttcata
     4861 aggggccaag attaccggat gattaataat gaatatacta tttacggaat catcaccgaa
     4921 tataggtggt caggaattac aggctgttgc tcaaatgaag gccctgaaga aaatggggca
     4981 ttcagttctg cttgtctgca gggaaaacag caaaattgct tttgaagcca gtaaattggg
     5041 gattgatatc acattcgcgt tatttcgaaa cagtcttcac atccctactg catggagatt
     5101 actcggaata gttcatggtt ttcagcccaa tgcaatcgtt tgtcacagtg ggcatgatag
     5161 caatattgtt ggtttagtac ggttattcac taggaaacat ccattcagaa ttatcaggca
     5221 aaagacatat ttgacacgaa aaacaaaagt tttttcaata aatcattttt gcgatgaggt
     5281 gattgttccc ggaacaagta tgaagacaca tctggagcag gaaggatgtc gaacccgggt
     5341 tactgttgtg cctccaggct ttgatttcca gaaattatac gttgattccc gaaacagttt
     5401 gcctccaaat gttctttctt ggctggcgtc ccgaagggga tgccccgtta ttgctcaggt
     5461 aggaatgttg cgcccggaaa aagggcacga atttatgttg aatttactgt tccatttaaa
     5521 aatgaatgga cgacagttct gttggttgat tgtggggtct ggttcgcctg aactgcggga
     5581 gcatttacag tatcagattg acagtatggg catgcatgat gatgttttta ttgctgacaa
     5641 tgtttttcct gccgcccccg tatatcgggt tgccagtctg gtggttctgc cttcagaaaa
     5701 cgaatcgttt ggtatggtac tggcagaagc atcggcattt tctgtgcctg tactggccag
     5761 tcagattggt ggaatccctg atgttattca gaataaccag accgggacat tgttaccagc
     5821 aggtaataag cacgcatgga tgtgcgccct gaatgatttt tttaatgacc ctgggcgttt
     5881 ttatcagatg gctcgccagg caaaacagga tatagaagag cggtttgata ttaataaaac
     5941 tgcgttaaaa atactcacat tagcgaagca caagtaacaa tatgttttct gtaagtaact
     6001 tatcatttat cggtttcctt aaaaggattg ttttttcctc agattcactc ccggggaagt
     6061 gggaacacag aaaattccgg ttcatgtaca ttttgcgatg tgctataaat ccggttgcca
     6121 gtattcgata ttattacgaa ctgcgttcct tgcagtgcat tgaggatatt ctggcaatac
     6181 aacccacgtt gccagcaaga attcatcgac cttatttgca taaagggggg cgagcctggt
     6241 cccgggggca atatattctt gagcattatc gctttgtgca aaatttgcca gaaaaatatt
     6301 ctgaattttt gtttccacaa aaatcggtat cattagtcca attcatcggg aaagatggag
     6361 aggattttga tatccagtgt tctcccagtg gttttgaccg ggaaggagag ttgatgttgt
     6421 cattgttttt caataaaata gtgattgccc ggttaacatt ttcagtcatt ctgactcaaa
     6481 atggacacac cgcttttatt ggcggattac agggagcccc caaaaatacc ggacctgata
     6541 ttattcggtg cgcaacccgg gcctgttatg ggcttttccc aaaacggatt atttttgaag
     6601 cattttgtgc gttgatgaaa gcttgtaatg tctctgagtg tctggcagtc agtgagcaca
     6661 gtcatgtttt ccggcaattg agatactggt atcagaaacg caaaaccttt gttgctgtct
     6721 acagtgattt ctgggagtct gtagcaggga aaacctgtgg tgactggtac aaattaccaa
     6781 cacaggtggt tcgaaagccg ctaagcaata tagctagtaa aaaacgctct gagtatcgaa
     6841 aacgatacgc tttgctggat tatatccatg aaactgctat ccgctctttg gatgcatatc
     6901 ctgtaaactc agaacatcag gacttaaatt aaggtttgat gtattctctg aataaaatat
     6961 taatgatgat tttggtaggg gcattcgcac taaataatga aaaaatacaa atccgagttt
     7021 attcctgaat ttaagaaaaa ttatctttcc cctgtttact ggtttacatg gttcgttttg
     7081 ggaatgattg caggtatttc aatgtttccc ccttcattca gagatcctgt cttggccaaa
     7141 atagggcgtt gggtgggcag attgagcaga aaagctcgtc gcagggcgac gattaattta
     7201 tcgctttgtt tcccggaaaa gagtgataca gaacgggaaa taattgtcga caatatgttt
     7261 gccacagcat tgcaatccat agtgatgatg gcagaactgg caattcgtgg tccggaaaag
     7321 ttccagaaac gagtgttctg gaaaggcctc gaaattcttg aggaaattcg gcacaataac
     7381 agaaatgtga tttttctggt tccccatggc tggagcgtgg atattcctgc aatgttgctg
     7441 gcagctcagg gggaaaaaat ggccgccatg tttcatcagc aacgaaatcc agtgattgat
     7501 tatgtctgga attctgtacg gcgtaaattc ggggggcgct tacattcccg ggaggatggg
     7561 ataaaaccat ttattcagtc agtacgccag ggatactggg ggtattacct tccagatcag
     7621 gatcatggtc ctgaatacag tgaatttgct gatttttttg cgacctataa agcgacatta
     7681 ccaattattg gacgtctgat gaacatcagt caggctatga ttataccgct tttcccggtt
     7741 tatgatgaaa aaaaacattt cctgactatt gaagttcggc caccaatgga tgcatgcatt
     7801 gccagcgcgg acaataaaat gattgcccga caaatgaaca aaacagtgga aattttggtg
     7861 gggtcacatc cggaacagta tatctgggtt ttaaaattgt taaaaacgcg caaatcaaac
     7921 gaagcggacc cgtacccttg aaattataaa gtacaggtat ttccactagt tgtttcttac
     7981 aggttaccaa tcgaaacaca tcccccttcc gtgtgttgcc cgtttggcac ggaggactgt
     8041 gtagatgcag ttcgttaaat ttaatttatg
//
LOCUS       DAZXUL010000168         7909 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZXUL010000168 DAZXUL010000000
VERSION     DAZXUL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.168"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..251
                     /locus_tag="ACH3L1_003862"
     CDS             <1..251
                     /locus_tag="ACH3L1_003862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078408.1"
                     /translation="DSYVPHKAFFSLSLYGTSWNRIFDLVNRDICWGGAICSLKRETR
                     LSARNTEIKKPGHFFTKWPGKNNFYFLTVLSLIEMSQN"
     gene            complement(256..331)
                     /locus_tag="ACH3L1_003863"
     tRNA            complement(256..331)
                     /locus_tag="ACH3L1_003863"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(296..298),aa:Asn,seq:gtt)
     gene            complement(432..1903)
                     /gene="yeeO"
                     /locus_tag="ACH3L1_003864"
                     /pseudo
     CDS             complement(432..1903)
                     /gene="yeeO"
                     /locus_tag="ACH3L1_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            2064..2139
                     /locus_tag="ACH3L1_003865"
     tRNA            2064..2139
                     /locus_tag="ACH3L1_003865"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2097..2099,aa:Asn,seq:gtt)
     gene            complement(2177..3127)
                     /gene="cbl"
                     /locus_tag="ACH3L1_003866"
     CDS             complement(2177..3127)
                     /gene="cbl"
                     /locus_tag="ACH3L1_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFT4078409.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3229..4146)
                     /gene="nac"
                     /locus_tag="ACH3L1_003867"
     CDS             complement(3229..4146)
                     /gene="nac"
                     /locus_tag="ACH3L1_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFT4078410.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4474..4549
                     /locus_tag="ACH3L1_003868"
     tRNA            4474..4549
                     /locus_tag="ACH3L1_003868"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4507..4509,aa:Asn,seq:gtt)
     gene            complement(4605..5540)
                     /gene="ldtA"
                     /locus_tag="ACH3L1_003869"
     CDS             complement(4605..5540)
                     /gene="ldtA"
                     /locus_tag="ACH3L1_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFT4078411.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5602..6681)
                     /gene="cobT"
                     /locus_tag="ACH3L1_003870"
     CDS             complement(5602..6681)
                     /gene="cobT"
                     /locus_tag="ACH3L1_003870"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFT4078412.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6693..7436)
                     /gene="cobS"
                     /locus_tag="ACH3L1_003871"
     CDS             complement(6693..7436)
                     /gene="cobS"
                     /locus_tag="ACH3L1_003871"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFT4078413.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7433..>7909)
                     /gene="cobU"
                     /locus_tag="ACH3L1_003872"
     CDS             complement(7433..>7909)
                     /gene="cobU"
                     /locus_tag="ACH3L1_003872"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFT4078414.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2186 a         2122 c         1713 g         1888 t
ORIGIN      
        1 gcgacagcta cgtcccgcat aaggcatttt tctctttatc tttgtacggt acttcatgga
       61 acagaatttt tgaccttgtg aatcgtgata tctgttgggg aggggcaatt tgctcactga
      121 agcgtgagac tcgattaagc gcacgaaaca cagaaatcaa aaaacccggc cactttttta
      181 caaagtggcc gggtaaaaat aatttttatt ttttaactgt cctgagtctc atagagatgt
      241 ctcaaaacta aaatttggct cctctgactg gactcgaacc agtgacatac ggattaacag
      301 tccgccgttc taccgactga actacagagg aatcgtgaga acgaggcgaa tattagcgat
      361 gcccacccac aatgtcaaag cctgtttttt aaatttgaaa tcgtttgctg aaataatctg
      421 cattttgtcg tttattccga cacaactggc tttttttcac acttttgcgg ctcggatcga
      481 gggtatttcc acagccaacg tccagtaacc attcgccagt aaaacagcac ggcccgcaca
      541 gcccagtcgg caaacattcc catccagaca ccaaccacac cccagccaag catgattccc
      601 tgcacataac cgactacaac ccggcaaccc cacatgctca acatagaaac ccacatggcg
      661 taacgggcat cacgagcacc cttaaatcca gcgggtagca cccatgaggc ggaccaaata
      721 ggcataaata aagcatttag ctaaatcaga atcacaacga cgtgtttaac ctgtggatcc
      781 tgggtgtaaa acgatgccat aaccccggca aagggagccg ttagccaggc gatggccgtt
      841 aatccaagag tggacagcca gaacacatgc cgcaactgaa tctctgcttg cgctatctgc
      901 cctaccccca accttcggcc tgtaatgatc gtagaagcag aaccgagcgc acttccgggt
      961 aagttgataa gagccgcaat tgagaacgcg ataaaatttc cggcaataac gctggtcccc
     1021 atcccggcaa cgaacatttg ggttaataac cgaccactgg taaataacac tgattcgaca
     1081 ctcgctggaa taccaatccc catgacttcc cagataatgc taaaattcag cggtttaaaa
     1141 tagctcttta acgaaatcct tagcgcagga ttaaaaccaa tcgccagcac ccacaaaatt
     1201 gcaactgcgc caatataacg agaaatggtt aaacccaacc cagccccgac caggagaaaa
     1261 ggccgtaaat caatatgccg ctaataataa tattaagaat attcaggcta ccgttaatca
     1321 gtagcggtat tttcgtattc cctgcaccac gaagtgcccc gctaccaata agagtgatgg
     1381 cagcggctgg ataactgagt acggtcagct ccagataagt caacgccagt gctttaactt
     1441 ctgtcgtggc atcacccgcg acgaaatcaa taatttgttc gccaaaatga tgaattagcg
     1501 ttgccaacag tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg
     1561 ctcgtcgtcg atcccgctta ccgagactaa atgctacaac gacagtagta ccaagatcga
     1621 tagctgcaaa aaaagccata atgaccatat tgaagctgtc cgccaacccc acgccggcca
     1681 tcgcatcttt tcccagccaa ctgaccagaa aagtgctcag gacccccatc aacaggacac
     1741 aggcgttctc catgaagata ggaacagcaa gcggggttat ctcgcgctag aacaacactt
     1801 tgtagctctt gcgtttagag tgccagcgag tgccgtgaac aacctggcgt aaagcagagg
     1861 agatattcaa agccgacctt aattgcagaa agtgaaacca catttcaaat gatgagggag
     1921 aatcagcaaa gctgcaaaga ttttcgccaa caaattgtct gcaaatgcaa caaactgttg
     1981 atagaaacgg caaacagttg ggaaatttaa aaatcgggtt tgacaaaata tttttcgccg
     2041 ttaagatgtg cctcaacagc gattcctctg tagttcagtc ggtagaacgg cggactgtta
     2101 atccgtatgt cactggttcg agtccagtca gaggagccaa atttctgaaa agcccgcttt
     2161 tatagcgggc tttttgctat atctgataat caatttcctc ttcactgttt tccatcacct
     2221 ggcgcttgat atcctcaact gacagtcctg cattacaaag ttccagaaag cgccaaacat
     2281 agttacgctg aagttgtcct cgcttcagtc ccaaccagac agtattagca tcgaaaagat
     2341 gccgcgtatc caggcggatt aaattctctt cctcttgttc gccgctggat tgttcggcaa
     2401 ctaatccgat cccaagccca agagcaacat aggttttaat gacatcagaa tcctgtgcac
     2461 ttaatacaat atctgccggc aaacctttgc gggtaaatgc gtcatcagtg cgtgagcgcc
     2521 ccgtaattcc ctgtcggtaa gtaattaacg gccacttcgc tattgattcc agcgtcaatg
     2581 gtgtaatttg cgttaaggga tgatcgagtg gaacaagcaa actatggtgc caacaaaacc
     2641 atgggaaggc gacgagctgc gggtcattac tcaaacgctc gctggcgata ccaatatcag
     2701 cttcgccatt ttgcaacaac gtcgcaattt cctgtggcga accctggatt agctcgagcc
     2761 gaacctccgg gaaaagttcg cgaaaagctt taatgacctc tggcaagcta taacgtgcct
     2821 gagtatgcgt cgttgcaata gtgagaacac cagacgtatc gttggtaaac aggtctgcaa
     2881 gccgacgaac attactggct tcattcagaa tacgttctgc aatgaccagt aatgctttgc
     2941 ccggttcagt catccccagc agtcgcttac ctcgtcgaac aaatatttcg atgccaaatt
     3001 catcctccag ttcccgaata tgacggctaa cgcctgactg tgaggtaaaa agcatattcg
     3061 caacctctgt caggttgtaa tcctgacgtg cagcctcgcg gattatcttt agttgttgga
     3121 aattcacggt aaactccggg cagttcagat atcccgttat tgttaaagtc taatgcccgg
     3181 cataacaaat aataaaaacc cgcatcttat tccatcccga tataacgctt agctcaccaa
     3241 ttgccactgc cttttttcca tcactggaga actaatcact gacattaaca actctttcac
     3301 tgcctgtgcc tgtggtgaca agtttgctct ggcgggtaaa tttaaggaca aagagagact
     3361 cattgaagga gtggtaatgc gtgacatcca cccatttact gcgccacata acgaacgcgc
     3421 agccgattca ggtaatactg caacgcccat gccgctggca atcgctgcgg taagcgtggc
     3481 aatagactca atttcaccaa tgacttttgc cgtgagccgc cgtagggaaa aagcctcatc
     3541 aacacgaagt ctaacagcgc tgtaatcact ggggagaaaa aggttcattt gcgcaatagc
     3601 attaacatca acgttttgcc ccgggcaatc ttgagttcct accaggaaaa gatcttcttt
     3661 cagcaaagcc tgactggata ccccagccac aggggaatgc tcataaatca ccgccatatc
     3721 gagttggtga tttatcagtt tttcgttaag cactgcacca ctattttcat gaagatagat
     3781 aacgacctcc gggaattcag cgcgaaccgc ctgtaataag ggcatggtga tggatgacgc
     3841 agcggttcct ggcgcaaagc caatcgagac ttgccccgat aatgactgac caacgttatg
     3901 caccgccagt tgggcctgct cacactgacg taaaatggcc cgtgcatggg tatagagaat
     3961 ttttccggcg tctgttggcg taacgccccg ttttgtgcga atcaaaagct gttgatttaa
     4021 ctcaccttcc agtatggcaa cctgttggct gagcgctggt tgtgcgatat gcaatacttc
     4081 agcagcctgg gtcaggctac caatatctac aatttttacg aagtatttca ggcgtctgaa
     4141 gttcatgttg cctccggttt ttaagaatcg gcccaagtgc cgccattact tacaaccagt
     4201 atgcaagatg cctgccagtt ctattttggc gttgatgcgc aagctaacca actgtcaaat
     4261 aagagattat gatagattcg tcattggccc tttttatcag cagtcgcatt cacctgccct
     4321 ataaaaggag ggtatgcacc acgatggttc attacccaat aagattgaaa gctcaccact
     4381 ttgttgaaat tggcagcaaa caaacaaaaa aaatgcattt caccctttga catcaccatg
     4441 cactgccatt aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg
     4501 cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa attcaaaaaa
     4561 gcctgctttc gagcaggctt tttactttct aattaccaac gctcttaaaa catctgtctt
     4621 gaaccagaac taatttgcac aggcattccc gatcgatgtt gcaacgcagc atttgcgcga
     4681 tttacatcaa cttcttgccc gttgataaac gcccgcaaag atggggttac cggcaatggc
     4741 acttttcggt cagactcata ttctgcacga ttgcgcgaca gcggctcatg aacttccagc
     4801 cactttgaac catccggttc cgttgtgtat ttcactggct gatcaataat ttgtacacga
     4861 gtgcctacag gaacattatc aaacagatat ttgatatcgt cattacgcag acgaatacag
     4921 ccctgactta cacggagccc aataccaaaa ttggcattgg taccgtggat ggcatacaac
     4981 ctgccaatat aaatcgcgta cagccccatg gggttatcgg gccccgcagg aacaaatgcg
     5041 ggcaaactct cccctcgttt cgcatattcg cgccggatgt tcggcgttgg cgtccaggtt
     5101 ggtgcttctt gtttacgttc aacggtagtc acccagttac gcggggtttc tcgcccagcc
     5161 tggccgatac caataggaaa gacttccact gtattactgt ctggcggata gtaataaaga
     5221 cgcatttcag cgacgttaac cacaatccct ttacgaacag tggcgggcaa aatcagttgc
     5281 tgcggaatgg tgagttgtga gccaggcttc ggcaaaaaaa catcagcgcc cggattcgct
     5341 tccagcatat tacttaaccc ttgcccgtat tgtgcggcaa aagtctccag cggctgagta
     5401 ttgtgatcag gaacagttac agtaagcgac tgccccacta aacggctacc ctctggaggt
     5461 aatggataag ttaccgccag gctattttgg ctggcaaaaa gcagagcaaa tgagcaaaga
     5521 atatttacac gacgcatcat gtccctttcc taagtcgcga atactatccg ataagtatag
     5581 cttttatcag acttttcgtt tttagctgtt caaatcagaa gtcgtattcc ccggtagaac
     5641 aatattactg gcagcaagtt cgcccatgtt gttgtatatc gcacaagcag cttcgatgat
     5701 ggacatcgcc agagctgcac cactcccctc acctaaacgc atatccatat tgagataagg
     5761 ctccagcccc aaatgcgaga gcgctatacg cgcgcctttt tctgccgaca agtgagaagg
     5821 aatgagatac ggtttgattg caggagacat ctggcaggct gcgagcgcag cagcataaga
     5881 aagaaatcca tccagcaaca cgggtaaacc gcaggaagca gcacctaaca tcactccagc
     5941 tattccgacc aaatcaaatc cacccacttt tgccaggaca tcaacaccat cctgaggatt
     6001 tggttgattc aacgtaatcg cccgacgcac aacatcaatt ttattagcca gtttatctgt
     6061 cggcaggttt gcgccaatcc caaccacttc ttcaggatcc cggccagtga ttgtgctgac
     6121 tattgccgct gccggtgtcg tgtttgccat ccccagttca cctacaccaa acagcgtgac
     6181 accgtttttc gccagctccc gcgtataaca tatgacgtcc aaaagcaact tttcagcctg
     6241 acggcgactc attgccggag ctgaagcaat attgccgcta cctcgtgcga cacgcatgtt
     6301 gataagcccg gggataggct cagcagtatc aataccaaca tcaattacgt ggacgttagc
     6361 gcccgcttgt gctgccagca cacacacgcc ggttgttcca cgggtcatat tttcagcctg
     6421 tatggctgtc acttcttttg gagaaatagc gaccccttcc tcccagacgc cgtgatcggc
     6481 acacataacc agtaccgctt ttttgcccac atgcggtatg ccattcaccc ccggcattcc
     6541 tgccagttgt atggcaagcg cctccagctt tcctaggcta ccaacaggtt tgaataaccc
     6601 gtcaacatgc cgttgtgcac gcgacatagc ggcagaattg atggctggga tcgtattcag
     6661 taaatcggca attatttgca tgtcacgttt ctttatagca gtgccagcag gaagaccagt
     6721 tcaccaagct ctatggctgc tcccagcgta tcgcccgttt gcccgcccag cgtacgtttg
     6781 agaagctgac cgaggatgaa aatcgccacc atcgtcacca ccatagcagc cacaccatgc
     6841 atacccagca ataatacggc agcaaaaata gcggctaagc cgagtgtgac gcaggtttgt
     6901 cgcccatcaa ttttgccaat gaacacattg ccaagccctt cttcccgcgc ataacgatga
     6961 cgatacatca ataatgcggc agtcccgcgg ctgaccgcac atgctgccgc cagcgatgca
     7021 aggatcggtt cgccacgcag agccaactcg cttaacacca gaatctttgc cagtaccaca
     7081 aaaatcaatg ccagaccgcc gtgggtgcct aaacggctat cacgcatgat ttccaacatg
     7141 cgatcgcgac tacgtgcaga aaatacgcca tcacaggtat cagcgaggcc gtccagatgg
     7201 aattccccgg tcatcagcgc cagcacaagt acgctaaaca gtgccgccag tggtgcgcca
     7261 caccatgcct gcagcaccat gaagaccagc ccgctaatcg cgccaagcaa taatccaatc
     7321 aaaggaaaag taataatacc gcgagaataa tgttcgaaat ccagtccctg ggaccagcga
     7381 cgcggtacag gcagacgcgt aataaatgag agcatcgccc aaaataattt gctcatttga
     7441 tttttactcc aatacccgaa accaccagcc atacttcatt tgccgcagcg gccaactgct
     7501 gatttacccg cccggcaata tcacgaaaat gtcgtgccag acgactctcc ggcacaatcc
     7561 ccattcccac ttcgttagtc actaatacaa cctttgcggg gcaacgttgg caggcagcaa
     7621 tcaacgactg aatctcagca ttaatcgcct gttccatcgc ctgataatcc cattcatcag
     7681 ggtctttatc gccgccataa tcaaacaaca gattagtcac cattgtggta acgcattcca
     7741 gcaacacagc ctcattcggg ttaatgtctg catgaattaa ttcatcaaga tgttgccagc
     7801 gctccactgt gcgccagtgc tccgggcggc tttgccgatg atgttctatc cgtgcagcca
     7861 tctcatcatc aaggatttgc gaggtagcga tatacagaac ctgtgaaga
//
LOCUS       DAZXUL010000169         7747 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZXUL010000169 DAZXUL010000000
VERSION     DAZXUL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.169"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1015
                     /gene="ydfJ"
                     /locus_tag="ACH3L1_003873"
     CDS             <1..1015
                     /gene="ydfJ"
                     /locus_tag="ACH3L1_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFT4078415.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1104..2564
                     /locus_tag="ACH3L1_003874"
     CDS             1104..2564
                     /locus_tag="ACH3L1_003874"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFT4078416.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="ACH3L1_003875"
     CDS             complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="ACH3L1_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFT4078417.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2981..3667)
                     /gene="rspR"
                     /locus_tag="ACH3L1_003876"
                     /gene_synonym="ydfH"
     CDS             complement(2981..3667)
                     /gene="rspR"
                     /locus_tag="ACH3L1_003876"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFT4078418.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="ACH3L1_003877"
     CDS             complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="ACH3L1_003877"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFT4078419.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4639..6684
                     /gene="dcp"
                     /locus_tag="ACH3L1_003878"
     CDS             4639..6684
                     /gene="dcp"
                     /locus_tag="ACH3L1_003878"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFT4078420.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6729..7247)
                     /locus_tag="ACH3L1_003879"
     CDS             complement(6729..7247)
                     /locus_tag="ACH3L1_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFT4078421.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7523..>7747
                     /gene="ydeI"
                     /locus_tag="ACH3L1_003880"
     CDS             7523..>7747
                     /gene="ydeI"
                     /locus_tag="ACH3L1_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFT4078422.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1942 a         1813 c         1957 g         2035 t
ORIGIN      
        1 catcttgctg gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg
       61 tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt
      121 tgtggctatg ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt
      181 cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctggcgag
      241 cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt
      301 tgagaaggtt aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt
      361 ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg
      421 tttaattcag actttccttg caggctattt agtacagacg ttattgttta acaaagcaat
      481 tccaacagat gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg
      541 ttggttatcc gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat
      601 tgtgctggcg tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat
      661 tatggttgca ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa
      721 cattaccatg gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa
      781 agaaattggt ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac
      841 catgacggaa tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt
      901 aatctctgcg ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc
      961 tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct
     1021 caatcccttc tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc
     1081 atatttaaat ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc
     1141 ctgtttatga tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc
     1201 accgtgcgca tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg
     1261 gatattatga ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcaag
     1321 ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg
     1381 tcgttaaaaa agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt
     1441 gtgaaccgca aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc
     1501 cggcgaccgg acagttaatg ctcgagcacc cgatggtcgc tgccgacgtg caaaatcccc
     1561 accagccgaa aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag
     1621 gacttcccgc atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc
     1681 gtgacgttgt cacttcctac gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag
     1741 ataacgtgac tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata
     1801 cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac
     1861 ctttccgcca gtgggttatc gaagataact ttgtagccgg acgtccggaa tgggaaaaag
     1921 cgggagccga actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca
     1981 acggcagtca ttcattcctg gcatatctgg gttatcttgc tggatatcag cacattaatg
     2041 actgtatgga agatgaacat tatcgtcatg cggcgtatgg catgatgttg caggaacaag
     2101 cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac
     2161 gctatagcaa cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga
     2221 aattgccaca gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg
     2281 atctgctggc gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg
     2341 gaaatccgat agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta
     2401 gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg
     2461 atttgccaga caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag
     2521 cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc
     2581 atcggcggca ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt
     2641 tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac
     2701 cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc
     2761 tgccagtggt gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc
     2821 tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata
     2881 ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg
     2941 ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt
     3001 cgctgttttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca
     3061 ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct
     3121 ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa
     3181 cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca
     3241 ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg
     3301 caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc
     3361 tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca
     3421 cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc
     3481 cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa
     3541 cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc
     3601 gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga
     3661 cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac
     3721 aagcaataac ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg
     3781 gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc
     3841 gttgacaccc accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga
     3901 taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca
     3961 cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag atccgtacgc
     4021 agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca
     4081 tacggccagc tgcctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc
     4141 atacccggta agacggcgcg cgtcatatat actaggcctt tgttgttggt atcaatcatc
     4201 gtttcccagt cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca
     4261 ttatttacca ggatatcaat attgctccac tcggcaggga gcgatgccag catctcttca
     4321 atagcggcgc ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct
     4381 gttaactcct gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga
     4441 ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc
     4501 atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg
     4561 ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct
     4621 cgcgttcagg agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg
     4681 tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag
     4741 ggaatgcaac aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat
     4801 ttcaacaata ctattctggc actggaacaa agcggagaat taattacccg cgttaccagc
     4861 gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag
     4921 ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg
     4981 cgggtagatg ctgtctggca gcgccgtgaa tccctggggc ttgatagtga atccatccgc
     5041 ctggtggagg ttattcatca acgttttgtc cttgccggag ccaaacttgc acaagctgat
     5101 aaagcaaaat taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag
     5161 cggttacttg cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca
     5221 ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat
     5281 aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc
     5341 gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat
     5401 gccaatgata cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca gcaggcgaaa
     5461 ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca
     5521 gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat
     5581 gaattagcct ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg
     5641 tgggactggg cattttatgc ggaacaggta cggcgggaga aatttgatct tgatgaggcg
     5701 cagctcaagc catattttga attaaacacg gtgttaaatg aaggtgtatt ctggaccgcg
     5761 aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttccggtcta ccatcctgac
     5821 gttcgtgtgt gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat
     5881 ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca
     5941 acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc
     6001 ggtgagcctg cgttgttact ctgggatgat gtaataacct tattccatga atttggtcat
     6061 acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg
     6121 cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta
     6181 ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag
     6241 aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc
     6301 gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat
     6361 gattttgaat tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc
     6421 tatcgcagca gttatttcgc ccatattttt ggtggcggat atgctgcagg ttattacgct
     6481 tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga
     6541 ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc tttccagagg taacagcgaa
     6601 gatctggaac gcctgcatcg acaatggcgt ggtaaggctc ctcagattat gccgatgctg
     6661 caacatcgtg gcttgaacat ataaacacca ccaatggctg gcctggagat atcgccaggc
     6721 cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct
     6781 aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata
     6841 tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg
     6901 tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc tgttgccatt
     6961 tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagactgctg gcttacgctg
     7021 aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt
     7081 gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta
     7141 gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata
     7201 gtgtcggtat ctgcgagttg tacaatttta tctttattaa tgttcactgt tttttgtcca
     7261 cgctattata tgtaaaggtg atttgctttt tctgtatgct cgtccaatat aataattttc
     7321 aaacatataa atccgattat tctgaaaaca tgtttaagac tcttcaatct tcttaacttt
     7381 agtgagtcaa attctctgaa tatttgacag ctagtgggct gattttaaat gccttataac
     7441 aattggcagt attcttattt ggcaattata aaaatagcaa ccagacttaa taagtcaaac
     7501 caaacgcaat taaggagtta gtatgaaatt tcagtccatt gtattagcaa gttttcttgt
     7561 catgccttat gcgttggcag atgaccaggg cggtttaaaa caggatgcag cgccaccgcc
     7621 gcctcatgca atagaagatg gctatcgcgg aaccgatgat gcaaaaaaaa tgaccgttga
     7681 tttcgcaaaa aacatgcacg atggcgcttc ggtttcacta cgcggtaatt tgatttctca
     7741 caaagga
//
LOCUS       DAZXUL010000170         7721 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZXUL010000170 DAZXUL010000000
VERSION     DAZXUL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.170"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..832)
                     /locus_tag="ACH3L1_003881"
     CDS             complement(<1..832)
                     /locus_tag="ACH3L1_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4078423.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     E"
     gene            1014..2438
                     /gene="sbcB"
                     /locus_tag="ACH3L1_003882"
     CDS             1014..2438
                     /gene="sbcB"
                     /locus_tag="ACH3L1_003882"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFT4078424.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2481..2708)
                     /gene="tsuB"
                     /locus_tag="ACH3L1_003883"
     CDS             complement(2481..2708)
                     /gene="tsuB"
                     /locus_tag="ACH3L1_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFT4078425.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2722..3780)
                     /gene="tsuA"
                     /locus_tag="ACH3L1_003884"
     CDS             complement(2722..3780)
                     /gene="tsuA"
                     /locus_tag="ACH3L1_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFT4078426.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="ACH3L1_003885"
     CDS             complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="ACH3L1_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFT4078427.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="ACH3L1_003886"
     CDS             complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="ACH3L1_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFT4078428.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5530..6513)
                     /locus_tag="ACH3L1_003887"
     CDS             complement(5530..6513)
                     /locus_tag="ACH3L1_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFT4078429.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6559..7383)
                     /locus_tag="ACH3L1_003888"
     CDS             complement(6559..7383)
                     /locus_tag="ACH3L1_003888"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFT4078430.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="ACH3L1_003889"
     CDS             complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="ACH3L1_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFT4078431.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2067 a         2012 c         1812 g         1830 t
ORIGIN      
        1 gttcgaagat tggacggcgg ataacattac ccatcttgct atgctctttc ggtacggtcc
       61 atacgttgtc cagcaggtca aattctgtct ttgttgccag cctaagctct gagagcctcg
      121 ccccccacag cataagcatc tgatgaagga gcttatttga cgtagacgca cggcttcttt
      181 caatagcaag ccaaatctta gccagttcgt gatacgacag tacccgatcc cctacctcag
      241 cgcgggagcc gaagtccctt ggctggatgc tcataattgc gcaactatct atcaactgac
      301 gccgcatgca ccaactaatt gctgatctta gttgacttag cacctgcctt gctcggcgtg
      361 gattatcttt ttcttcttcg gtaagcaggt caacccattg cttaaccgtg atagaagatg
      421 ccggacgatt agggaaggcg tcatgcatgc gcttcataac cgttgatcga taaagtgcct
      481 gggtcttttc tctgagagtt gtagagacgt agttgtcgaa ccagtagtcg agacactggg
      541 cgaccgtcat ggagttctcc accttctctt caaaataggt gcgtggatcc gttcctgaga
      601 aatagagctt tcgcaagtca gcagtgatct gtctggcatc cttcaaagac agggatgggt
      661 atcgaccaag cccaagtcga ttaggcttgc catgccagcg atagcggtac tggaactgga
      721 tgaccccctt cggtgaaatt cgtacgctga ggccatcggc atcagccact tcttgtgggc
      781 ccgaatatgg tttaccataa atagtacgca gttttgtgtc gcttatagcc ataaataata
      841 tttggtacgc tcagaaataa tagtttggca cacatctggt acacaatatc acatggacaa
      901 cagcgcacag taaacaacta tatttgacgt tcgtagacat actcaggtga aaaaagggtt
      961 gcttttcgag ttataacaga cagttaatta actacagtgg acaatgctag acaatgacag
     1021 acacagataa acaacctacc ttcctctttc acgattacga aacctttggc acgcaccccg
     1081 cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg
     1141 gcgaacccga agtcttttac tgcaagcccg cggatgacta tttaccccgg cctggagcag
     1201 tattaattac cggtattacc ccgcaggagg cacgggcgaa aggagaaaac gaagccgcgt
     1261 ttgccgcccg tattcactcg ctttttaccg taccgaagac ctgtattctg ggctacaaca
     1321 atgtgcgttt cgacgacgaa gtcacacgca acgtttttta tcgtaatttc tacgatcctt
     1381 acgcctggag ctggcagcat gataactcgc gctgggattt actggatgtt atgcgtgcct
     1441 gttatgccct gcgcccggaa ggaataaact ggcctgaaaa tgatgacggt ctaccgagct
     1501 ttcgccttga gcatttaacc aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga
     1561 tggctgatgt gtacgccact attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc
     1621 tgtttgatta tctctttacc catcgtaata aacacaaact gatggcgttg attgatgttc
     1681 cgcagatgaa acccctggct cacgtttccg gaatgtttgg ggcatggcgt ggcaatacca
     1741 gctgggtggc accgctggcg tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt
     1801 tggcaggaga catttcgcca ttactggagc tggatatcga cacattgcgc gagcgtttat
     1861 ataccgcaaa agccgatctt ggcgataacg ccgccgttcc ggttaagctg gtgcatatca
     1921 ataaatgtcc ggtactggcc caggcgaata cgctacgccc ggaagatgcc gaccgactgg
     1981 gaattaatcg tcagcattgc ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc
     2041 gtgaaaaagt ggtagcgata ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg
     2101 atgcacagct ttataatggc tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc
     2161 tggaaaccga gccgcgtaat ttaccggcac tggatatcac ttttgttgat aaacgaattg
     2221 aaaagttgtt gttcaattat cgggcacgca acttcccggg gacgctggat tatgccgagc
     2281 agcaacgctg gctggagcac cgccaccagg tcttcacgcc agagtttttg cagggttatg
     2341 ctgatgaatt gcagatgctg gtacaacaat atgccgatga caaagagaaa gtggcgctgt
     2401 taaaagcact ttggcagtac gcggaagaga tcgtctaatt tgaagccgcc gccgctggta
     2461 cggcggagga caatgttgag tcaggctttt tgaacggtga tgctccacgc tgcatcgcca
     2521 atctgctgat aatcggtgat ggcatgtcct tcttctgccg cccactgtgg gattgcttca
     2581 gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc cgctggccat ttctgccagt
     2641 gctgcttttg cttctatgag tggaaacgga cagacctgcg tcaccacatc cagcttttta
     2701 atcgccatct ttacacctta cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg
     2761 cggtcgaaca tacacaagcc aggacgcagt ccagactccg agaatcataa atacaaggcc
     2821 aatccagccc tgccaggtca tcattgcagt cataaccagt ccattaccga tagagcaacc
     2881 acctgcaata ctggcaccga agcccatcag tacaccacct aacccgctac gtaatgttgt
     2941 ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct ttggctgcaa taaatgcctc
     3001 cacgaagatc cctaacacca ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac
     3061 cagaaattga agaatattgg ccgttgggga agtgactcct aacccaaaca tgcgtccggt
     3121 tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa
     3181 gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg gcggtaaggt
     3241 cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg ttatcaccag
     3301 caaaacggta accaacggcc acacagacaa attaaaagtc tcagcaatag agttatgttc
     3361 agtagtgtag tgctgcaagg tttgatttaa accactggcg tgtggagaac gcatcaccgc
     3421 actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct cacccgcgcg
     3481 ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga aaagataccc
     3541 acctataaca gtaccaagcc acgggaacgc cccggcttcg taagtcagta ggcccgcctg
     3601 aattaacgca aaaaccccca cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat
     3661 gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg
     3721 ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca ttgaaaacat
     3781 aaattaatta accagatgaa tgttaatggg gaaatttttc atgactggtg gaatgcagac
     3841 caataaccaa attctctaaa ttagaacaaa tggttattaa tgaggatttc gataaaaaag
     3901 aataaaaaaa ccggagccaa atgttcattc gactccggtt tattattaga aaagatgatt
     3961 atgctacgtc ttcgtactgc ggaaccggat tacggaagct cttggtgacg caagccaggt
     4021 aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct tccaggttaa
     4081 cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat
     4141 ctttcagcgt cttgttacgc ttctcacgga tccagaactg cgagatgacc gacaggttaa
     4201 caaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt accaggtcga
     4261 agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc
     4321 atttcgggtg tacataaccg aagaagcttt tcgggaacac gccgtcacga cccattacgt
     4381 acatcagacg cgctacgcct gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg
     4441 agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct
     4501 gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag
     4561 ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca
     4621 cgcgctctgc atctttggtt tcttccgaca gattgctgat gccgtcaaag ccggtaaagg
     4681 agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg ccagaccaga
     4741 acggacgcgt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca taaacaacca
     4801 tgcccagaat caccgcgatc agcactacct gcaacacgac gattacagtg ttgacgttcg
     4861 ctacggattt cagactacgc aggttaaagg cggtcataaa ggccaccagc gccaccacaa
     4921 acatccacga tggaatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt
     4981 tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag
     5041 tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa
     5101 ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg
     5161 tcggcacatg accatccgtg aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg
     5221 gctgcatata ggctagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt
     5281 ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt tattctcctt tacggtgata
     5341 accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc
     5401 gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt
     5461 gaagcaaaaa ataaccgacg ctaattaaac gtcggttttt aatcattttt tgacagcggc
     5521 gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca taataatttt
     5581 tctttgaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcactatt
     5641 tgctaaaatt tcatctcgcc accagccgct ggcaagccct gcggtctgtt cattccagcc
     5701 aatccagaca ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact
     5761 atcgagaaaa cgttgcgcca gataccgtgg taaatagccg cagccaaggc cgctaatttg
     5821 cagctccagt ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg
     5881 tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc gatatcgttt
     5941 aatgatacga cggtttaacg gttcttcttc ctgggctaac ggatgatgag gcgcgaccgc
     6001 aaagacttgt tcaagatcgc ccaaccgcga gaaaccaaac tcactggaag atggtggctc
     6061 atgcatcgcg ccaacgataa tatccgctcg cccctgagtc agggcatccc aggaaccacc
     6121 gagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat
     6181 gagaggcgca agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc
     6241 ccagccttca tgcagtttga tcgcctgctt ttccagttct cgcacggtat gcagaacttc
     6301 ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt gtccgctacg
     6361 atcaagcaat tgaatattta ggtcgctttc cagcttgtga acggtgtaac tcagagcgga
     6421 cggcgtcttg tagagtttgg ctgacgccgc cgcaaaactg ccctcttttt ctatggcatc
     6481 gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt gcatgcggcg
     6541 tcctgctggc gtgatcgttt actccaacgg cattaccagc ggatcgggat actggtattc
     6601 aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag
     6661 actatttctg aactgcggag gttgcagccc cagtaaacgg gccatctgcg gatagaaaac
     6721 attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg
     6781 taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc
     6841 accatcaggg gcggtttttc cggcaaagaa gcgtcccgga tgacgtcccg gtccgaccag
     6901 gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag
     6961 ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt ctttaaccgt
     7021 gccttgcgcg tcgccataga cagatgttga gctggtaaaa ataattcggg gaatacgatg
     7081 ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggggcc
     7141 gctacgacgt gccggaagcg taatgaccag cgcatcggca tccataaggg catccagatc
     7201 gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa tgccactcat
     7261 tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc
     7321 tgaaagtgac atcgccagcg gcatacctaa ccaccctaac ccgacaattg cgaccttttt
     7381 catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga
     7441 caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca atgagcagtg
     7501 aatcatcagt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg gaccagaaac
     7561 ctgacaaatt atgtttcagg ggttctggtt tccccttacc ttcaaatggt gttctgcggg
     7621 tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc tgccagtaca
     7681 gataatcatc ccatgattcc tcagaccaga ttagtttcac t
//
LOCUS       DAZXUL010000171         7631 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZXUL010000171 DAZXUL010000000
VERSION     DAZXUL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.171"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            268..918
                     /gene="csgD"
                     /locus_tag="ACH3L1_003890"
     CDS             268..918
                     /gene="csgD"
                     /locus_tag="ACH3L1_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFT4078432.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            923..1312
                     /gene="csgE"
                     /locus_tag="ACH3L1_003891"
     CDS             923..1312
                     /gene="csgE"
                     /locus_tag="ACH3L1_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFT4078433.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1337..1753
                     /gene="csgF"
                     /locus_tag="ACH3L1_003892"
     CDS             1337..1753
                     /gene="csgF"
                     /locus_tag="ACH3L1_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFT4078434.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1780..2555
                     /locus_tag="ACH3L1_003893"
                     /pseudo
     CDS             1780..2555
                     /locus_tag="ACH3L1_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(2619..3110)
                     /locus_tag="ACH3L1_003894"
     CDS             complement(2619..3110)
                     /locus_tag="ACH3L1_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFT4078435.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3212..3766)
                     /gene="ycdY"
                     /locus_tag="ACH3L1_003895"
     CDS             complement(3212..3766)
                     /gene="ycdY"
                     /locus_tag="ACH3L1_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFT4078436.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3790..4527)
                     /gene="ycdX"
                     /locus_tag="ACH3L1_003896"
     CDS             complement(3790..4527)
                     /gene="ycdX"
                     /locus_tag="ACH3L1_003896"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFT4078437.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4582..5520)
                     /gene="ghrA"
                     /locus_tag="ACH3L1_003897"
                     /pseudo
     CDS             complement(4582..5520)
                     /gene="ghrA"
                     /locus_tag="ACH3L1_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            5755..5842
                     /locus_tag="ACH3L1_003898"
     tRNA            5755..5842
                     /locus_tag="ACH3L1_003898"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5789..5791,aa:Ser,seq:gga)
     gene            complement(6035..7021)
                     /gene="ycdU"
                     /locus_tag="ACH3L1_003899"
     CDS             complement(6035..7021)
                     /gene="ycdU"
                     /locus_tag="ACH3L1_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFT4078438.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(7018..7546)
                     /locus_tag="ACH3L1_003900"
                     /pseudo
     CDS             complement(7018..7546)
                     /locus_tag="ACH3L1_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         2182 a         1848 c         1670 g         1931 t
ORIGIN      
        1 acaacttttc tatcatttct aaacttaata aaaccttaat gttaacattt taatataatg
       61 agttacattt tgttacatgt ttaacacttg atttaagatt tgtaatggct agattgaaat
      121 cagatgtaat ccattagttt tatattttac ccatttaggg ctgatttatt actacacaca
      181 gcagtgcaac atctgtcagt acttctggtg cttctatttt agaggcagct gtcaggtgtg
      241 cgatcaataa aaaagtgggg tttcatcatg tttaatgaag tccatagtat tcatggtcat
      301 acattattgt tgatcactaa accttctttg caggcgacag ctctcttgca gcaccttaaa
      361 caatcgctag caattacagg aaaattacat aatattcaac gttctctgga cgatatctct
      421 tcaggctcta ttattcttct ggatatgatg gaagcggata aaaagcttat ccattattgg
      481 caggatactt tgagcaggaa aaacaacaat atcaaaatat tgttgctaaa tacgcctgaa
      541 gattacccgt accgcgacat tgaaaactgg cctcatatca acggcgtttt ttatgccatg
      601 gaggatcaag aacgtgttgt caatgggttg caaggcgtcc tgcgtggcga atgctacttt
      661 acgcaaaagc ttgccagtta cctgattaca cattctggta actatcgtta taacagcacg
      721 gaatcagccc tccttactca tcgggaaaaa gagatcctga ataagctgcg tatcggcgcg
      781 tctaataacg agatcgctcg ttcgttgttc atcagcgaaa atacggttaa aacgcatctt
      841 tataatcttt tcaagaagat agccgtaaaa aaccggacac aagcggtttc ctgggcaaac
      901 gataacctca ggcgataaag ccatgaaacg ttatttacgc tggattgtgg cggcagaatt
      961 tctgttcgcc gcagggaatc ttcacgccgt tgaggtagaa gtcccgggat tgttaactga
     1021 ccatactgtt tcatctattg gccatgattt ttaccgagcc tttagtgata aatgggaaag
     1081 tgactatacg ggtaacttaa cgattaatga aaggcccagt gcacgatggg gaagctggat
     1141 cacaataacg gtcaatcagg atgttatttt ccagactttt ttattcccgt tgaaaagaga
     1201 ctccgagaaa actgtcgtct ttgcactgat tcaaactgaa gaagcactaa atcgtcgcca
     1261 gataaatcag gcgttattaa gtacggacga tttggcgcat gatgaattct aaataaaaaa
     1321 ttgttcggag gctgcaatgc gtgtcaaaca tgcagtagtt ctactcatgc ttatttcgcc
     1381 attaagttgg gctggaacca tgactttcca gttccgtaat ccaaactttg gtggtaaccc
     1441 aaataatggc gcttttttat taaatagcgc tcaggcccaa aactcttata aagatccgag
     1501 ctataacgat gactttggta ttgaaacacc ctcagcgtta gataacttta ctcaggccat
     1561 ccagtcacaa attttaggtg ggctactgtc gaatattaat accggtaaac cgggccgcat
     1621 ggtgaccaac gattatattg ttgatattgc taaccgcgat ggtcaattgc agttgaacgt
     1681 gacagatcgt aaaaccggac aaacctcgac catccaggtt tcgggtttac aaaataactc
     1741 aaccgatttt taagccccag cttcataagg aaaataatca tgcagcgctt atttcttttg
     1801 gttgccgtca tgttactgag cggatgctta accgccccgc ctaaagaagc cgccagaccg
     1861 acattaatgc ctcgtgctca gagctacaaa gatttgaccc atctgccagc gccgacgggt
     1921 aagatctttg tttcggtata caacattcag gacgaaaccg ggcaatttaa accctacccg
     1981 gcaagtaact tctccactgc tgttccgcaa agcgccaggg cttacaaaac ctgcttaacg
     2041 agcgcaaggt tattcgtgcg gcacaagaaa acggcacggt tgccattaat aaccgaatcc
     2101 cgctgcaatc tttaacggcg gcaaatatta tggttgaagg ttcgattatc ggttatgaaa
     2161 gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca gacacgcaat
     2221 accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc ggtgagatcc
     2281 tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc ggggttttcc
     2341 gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca aacgaacccg
     2401 ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt aatgatggta
     2461 tcgaccgtgg tctgtgggat ttgtaaaata aagcagaacg gcagaatgac attctggtga
     2521 aataccgcca tatgtcggtt ccaccggaat cctgacagat aaaaatgccg gaattaataa
     2581 tccggcattt actgtttaag cgaaagcgtg agtgcccctc acgctttatt tttgatttcc
     2641 tgctcacggt gagccgtctt tgcactacgc gccgccagcc acaggccgct gtttttcatt
     2701 gcgtaaccaa ataccagccc aagcgccagc gaaggtaaca ccagtttcca gtcaccctgc
     2761 ccggcaaacg tcgcgcatgc gcctataaac gtccccggaa caaatgaaag cagcagctgt
     2821 ttggcctgaa tacacatcag aaacgcgaca ataccggtta tgacataacc gagaatttcc
     2881 agatgtggtg ccagtgcact accgtaaata atgaccatcg cccacaccac gccactaagc
     2941 agggttgcag cggagatcgc cagccctttc agcccacctt gcgggcaagc aaagtaggcc
     3001 gtacagccca ggaagcccgc ccagcttagt aagcccaggg aaacagccac ccatccccag
     3061 ataccggaga gaatgcctgt tgtgattgca atagagagaa gtatgttcat gccgcgcatg
     3121 atagcagaaa acgcggccat gaatgagatc gagcacacat tttatgcaac aacaaattat
     3181 ctgttgcagt tatgagtgac gatgatcaca tttactcttc agaatcttct tccagctcgt
     3241 cccacattgc actaatggca tcacgggtta gcggtgccat ggtgcgccag aaaggcgtgg
     3301 ttgcatgggc ctccactttg ccgaggaacg caccacacca gggtaacaga tactcactga
     3361 acagtgtttc cagtgcttcg ctctcatctt ccgttgactg atcttccagc caggaagccg
     3421 caagcagcaa tgtgccgatg tgatcggctg gcgtatccgc cagtagcatc cctcgttcgg
     3481 aaagaaaagc gcgcacttcc gcttccgttg cgtcctcaac ccatgcgcta cgatatggcg
     3541 gcacggcaca ttcatcgccg ataaacaacg cattgtaatc ggcagagact tgcgtcatat
     3601 cacagctttt ctgtaaacgc gtcagtaact catcctgctc cagtggccag ttcgcagcca
     3661 gtttcccctc acgaatcaga gtaaacagcg gcaccagtaa aggatcttgc ggttggcggt
     3721 aatagagcga acccagcaca cgacagagga tagaaaactc gttcattaat atatttccat
     3781 tactagtaat taaaggtctg caaattccgc aatcggtgcc ataccgcgag attcaaggaa
     3841 gttcagtaag cggcgcggag aaacattcaa aatgcgctct agcggaaaat ctaccgcgtc
     3901 gaggatttta agacactctt caaattcccc catggtaaac gcggtgtgag aatccgagcc
     3961 taatgccacc caacctccag catcacgtac cgctgcagct accgcacgac agttgtcttc
     4021 actgccctta cgtgagtgta aaaatgagga attattgatt tccagcgcca cctgatgttt
     4081 cgcggctgct tcagcaacgg ctttaacatc tatttcatat ttcggatttc cgggatggct
     4141 tattatatgc acattgccgc tggcgatagt ggagatcatc gcttgtgtat tggtcgcttt
     4201 gtcatgtggc gcaaaaaccg gctcatgaaa accggcaata atcagatcca gcgagtcaaa
     4261 cattttaccg ctgcagtcaa tttcaccatc aacattttta atgttagctt caatgccgcg
     4321 caggatccct accccatcaa ccactcgcgg ccagatacgc atgttaatga agtgccagtg
     4381 atgcggcgca tcttccatat ccgggccatg atcggtgatc gcaaaaagtt taatgccctt
     4441 ttgtttggcc tgggcaatgt aatcactcaa tgtgctatat gcatgtgtgc tggcaacggt
     4501 atgcatatga aggtcgacgg gatacataac tctctcctgt attcaattcc cgccaaggat
     4561 agcaggaatc ctgatgcttt attagtagcc acgtgcgcgg tcgacttgcc cgcagaccct
     4621 ctcccctttt tcgagctggg caatagtgcg agaaatgtac tccacagctt cagcgggacg
     4681 ggtaatcgcg gcgacatgtg gtgttatcgt cacgcgtgga tgttgccaga gcggactttc
     4741 aggcggtaag ggttcacgat taaaaacatc cagcattgcg cctttaactt tcccgctatc
     4801 cagcgccgcg agcaggtcat cttccacaac atgaacacca cgcgccaggt tgaggagata
     4861 cgcgccatca ggtaattttt cgagtaattg ttgattaata atgccgacgg tttcaggggt
     4921 attcggtaac aaattaatca atacccgaca ttggctcaga aatgcagaca gttcttcccg
     4981 tccggcaaag ctttgcacgc caggccacga tttacgggtt cgactccagc aacgcagcgg
     5041 aaagcgctag gtttgcagac tctgagcaac tttacagccc agtacgcctg cgcccaaaat
     5101 gccgatggta aaatcttccc gatgatattc aggcagcggt tgccaatgcg aactattttg
     5161 ctggatgcga taatcgtcaa aacgtcgaaa ccaatgcagc acctgactga cagcatattc
     5221 ctgcatttgc tcgcccatac cggtatcttc caggcgaaaa agtggaacag aagggttcag
     5281 catttcaggg tgtgcctgta gcttgctcaa aatagaatca acaccggccc cgagtgcgaa
     5341 caccgcttta agatcgcgcc ctgccagcat ttcaacagga ggatgccaga ctaaaacata
     5401 atcagcagag tcattatctc cgcttttcca tgccctgact cttgcctgag gaatagcttt
     5461 gcgcagtgcc tcaatccacc attgggtatc gaacgttggg tgataaaaga tgatatccat
     5521 actgactccc gaaaagcgtt gtgcgaaatt tattcgcact tatcgttatg atctacaaag
     5581 gccaccagca taacaaatcc gtggtcggtg gcaaaaaaag cagatttcgc ttattaaaac
     5641 cacacattga ttgaatgttt gaataaacgc gcgatttttt caaaaagttt gttgacctca
     5701 ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca atttggtgag
     5761 gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg tatcgggggt
     5821 tcgaatcccc ccctcaccgc catatttaaa gaagagcgcg tacgaaagta cgggcttttt
     5881 tttcgtatat tgtacacacc gggtgccccc gcccacgtct gttagggcga ggggaaattg
     5941 tgcacatcaa ctgtctggtt actcagtcaa caatcaaaat gcgtaagata gtacagaaca
     6001 tatatgcgtt atctggtcac ttcctgtaac ttcctcactt attaaaatgc ccccaacgga
     6061 aatacatgtc acatgccatc ataaaatcgt tatgccagag gaatcgacaa tggaaaaaat
     6121 agtaaattaa gcacggtaca tagcagagta aaaaataggc ttcaaaccaa atccgccatt
     6181 ccacaagcgt tgccaatacc gacaccagcg taatccacat catttcaata acccgcaata
     6241 tgaataaccg aagccaaagc ggcttggcaa aaaaggtcga caacgtattt tcactggaga
     6301 aaaacagatc gatttcctgc aaaaaaaacc tgcccagaaa agcgggccgc ttggagaagc
     6361 gcagaaaaaa cagattaatc gccaacacta cgccacaaag aatgagcaat gcaattatcg
     6421 atttaaacgt taaccactgc ccctctgtca caaaagaatc actgttacgc agcgggctga
     6481 tagtaagcag gttaacaacc gttgagatgc acaccgaaag cagcacatag cgagtcagac
     6541 ttttatattt tacttttttc gctactgaaa tagcgacctg cttgacataa ctggtgcgga
     6601 tcatccgaaa cagcagaaag atgaccaacg gcccaagtaa cgaaatcagg aacagcgcat
     6661 ataacgaggg tagaagctgg tatagccccc aactcattgc actcaacatg attaacgaca
     6721 gaaatcctgc caccagaggt tgtgtaagaa ataattctat tttgtggttc tgctttttct
     6781 taaaccacga aaaatcgata ttcttcgtga cgcatttata agcccagtac gccttgagat
     6841 caaaaataaa attatacagc accagggcca gaatcccact aactacacca atatcctctg
     6901 atggaaaagt aaaccctgca tttttccaat aaaagcctat cagaataagt ccaatagcat
     6961 aaatgtatat cagatttcgt ttatcctgaa agcgaataaa agttaaataa tcaggaatca
     7021 tttgaatacc tttgcatcac taaatgcttt acgacaatgc ataactcgtt gctgatccac
     7081 gccagcaaaa tcatttaaca tattccccaa atagggctta ttacttattt caaggaaagc
     7141 taccggggtt ggcatattca gaatatgtat cgagagattc agtccatttc accgtatgag
     7201 ttaagtgtaa tgagagattt tctttgataa ttgacggagc gacctcgctt tttgctgtta
     7261 cgttcatcag aacctgatgc tctggcgaag cgatatccag tccggcaaga taatcgcgca
     7321 ttgcctgaac gccgtcttcc atcaaacgcg tgtgccaggc accgctcaca ccgagtttaa
     7381 ccggttcgta tccagcggcc atcagcagcg tggtaaattc attcaacgca gcctgcgttc
     7441 ccccaataac ctgctggcgc ggcgtgttat cacagctaat atccagcgca atgcctgatt
     7501 ccgtaatcat cgtctgcagt tgttcgcggt tgatgccttt aaccgcctga tcttacccag
     7561 caatagtgga cacgcggcta agtgagtaaa ctctcagtca gaggtgactt acatgacaaa
     7621 aacagtatca a
//
LOCUS       DAZXUL010000172         7539 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZXUL010000172 DAZXUL010000000
VERSION     DAZXUL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.172"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="ACH3L1_003901"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="ACH3L1_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="ACH3L1_003902"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="ACH3L1_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFT4078439.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="ACH3L1_003903"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="ACH3L1_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="ACH3L1_003904"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="ACH3L1_003904"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFT4078440.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="ACH3L1_003905"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="ACH3L1_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFT4078441.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="ACH3L1_003906"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="ACH3L1_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFT4078442.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="ACH3L1_003907"
     CDS             complement(6510..7202)
                     /locus_tag="ACH3L1_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFT4078443.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1916 a         1828 c         1986 g         1809 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccgggat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagaaaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc ctccctcgcg ccgctgagaa aaagcgaag
//
LOCUS       DAZXUL010000173         7379 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZXUL010000173 DAZXUL010000000
VERSION     DAZXUL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.173"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1545
                     /locus_tag="ACH3L1_003908"
     CDS             <1..1545
                     /locus_tag="ACH3L1_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFT4078444.1"
                     /translation="FTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREV
                     VDTYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARF
                     GVYSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDN
                     FTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIG
                     VVDRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGV
                     KRILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWW
                     KGKLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLT
                     WITSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTV
                     ENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKK
                     ALSEAK"
     gene            1597..2754
                     /locus_tag="ACH3L1_003909"
     CDS             1597..2754
                     /locus_tag="ACH3L1_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFT4078445.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2720..2842)
                     /locus_tag="ACH3L1_003910"
     CDS             complement(2720..2842)
                     /locus_tag="ACH3L1_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078446.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3045..4730
                     /locus_tag="ACH3L1_003911"
                     /pseudo
     CDS             3045..4730
                     /locus_tag="ACH3L1_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4768..7128
                     /locus_tag="ACH3L1_003912"
     CDS             4768..7128
                     /locus_tag="ACH3L1_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFT4078447.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7185..>7379
                     /locus_tag="ACH3L1_003913"
     CDS             7185..>7379
                     /locus_tag="ACH3L1_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078448.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT         2164 a         1615 c         1568 g         2032 t
ORIGIN      
        1 tttacgccga ctgaatacag taccaaagga aagccaaata ttatcgtact aaccatggat
       61 gatcttggtt atggacaact tccttttgat aagggatctt ttgacccaaa aacaatggaa
      121 aatcgtgaag ttgtcgatac ctacaaaata gggatagata aagccattga agctgcacaa
      181 aaatcaacgc cgacgctcct ttcattaatg gatgaaggcg tacgttttac taacggctat
      241 gtggcacacg gtgtttccgg cccctcccgt gccgcaataa tgaccggtcg agctcccgcc
      301 cgctttggtg tctattccaa taccgatgct caggatggta ttccgctaac agaaactttc
      361 ttgcctgaat tattccagaa tcatggttat tacactgcag cagtaggtaa atggcacttg
      421 tcaaaaatca gtaatgtgcc ggtaccggaa gataaacaaa cgcgtgacta tcatgacaac
      481 ttcaccacat tttctgcgga agaatggcaa cctcaaaacc gtggctttga ttactttatg
      541 ggattccacg ctgcaggaac ggcatattac aactcccctt cactgttcaa aaatcgtgaa
      601 cgtgtccccg caaaaggtta tatcagcgat cagttaaccg atgaggcaat tggcgttgtt
      661 gatcgtgcca aaacacttga ccagcctttt atgctttacc tggcttataa tgctccgcac
      721 ctaccaaatg ataatcctgc accggatcaa tatcagaagc aatttaatac cggtagtcaa
      781 acagcagata actactacgc ttccgtttat tctgttgatc agggtgtaaa acgcattctc
      841 gaacaactga agaaaaacgg acagtatgac aatacaatta ttctctttac ctccgataat
      901 ggtgcggtta tcgatggtcc tctgccgctg aacggggcgc aaaaaggcta taagagtcag
      961 acctatcctg gcggtactca caccccaatg tttatgtggt ggaaaggaaa acttcaaccc
     1021 ggtaattatg acaagctgat ttccgcaatg gatttctacc caacagctct tgatgcagcc
     1081 gatatcagca ttccaaaaga ccttaagctg gatggtgttc ccttgctgcc ctggttgcaa
     1141 gataagaaac aaggcgagcc acataaaaat ctgacctgga taacctctta ttctcactgg
     1201 tttgatgagg aaaatattcc attctgggat aattaccaca aatttgttcg ccatcagtca
     1261 gacgattacc cgcataaccc caacactgag gacttaagcc aattctctta tacggtgaga
     1321 aataacgatt attcgcttgt ctatacagta gaaaacaatc agttaggtct ctacaaactg
     1381 acggatctac agcaaaaaga taaccttgcc gccgccaatc cgcaggtcgt taaagagatg
     1441 caaggcgtgg taagagagtt tatcgacagc agccagccac cgcttagcga ggtaaatcag
     1501 gagaagttta acaatatcaa gaaagcacta agcgaagcga aataactaaa ccttcatgcg
     1561 gcggattttt ccgccgcctt attgagcgag atagcgatgc acgttacagc caagccctcc
     1621 agttttcaat gtaatctcaa atgtgattac tgtttttacc ttgaaaaaga gtcgcagttt
     1681 actcatgaaa aatggatgga tgacagcact ctgaaagagt tcatcaaaca atatatcgaa
     1741 gcgtctggca atcaggtcta ttttacctgg caaggcggtg aacccactct ggctggcctg
     1801 gattttttcc gtaaagttat tcactatcaa caacgctatg caggccaaaa acgtattttt
     1861 aatacattac aaacgaatgg cattttattg aataatgaat ggtgtgcctt tctcaaagaa
     1921 catgaatttc tggttggtat ctcgatcgat ggcccccagg agttacatga ccgttacaga
     1981 cgcagtaatt caggtaacgg tacttttgca aaagtgatag cagccatcga gcgtctgaaa
     2041 tcatatcaag tagagtttaa tacgttaacc gtcattaata acgttaatgt ccattatcct
     2101 cttgaggttt atcatttttt aaaatctatc ggcagtaaac atatgcaatt tatcgaattg
     2161 ctagaaaccg ggacgccgaa tattgatttc agtggtcata gtgagaacac attccgtatc
     2221 attgattttt ctgtgcctcc cacggcttat ggcaagttta tgtcaaccat ttttatgcaa
     2281 tgggttaaaa acgatgtggg tgaaattttc attcgtcagt ttgaaagctt tgtcagccgt
     2341 tttttgggga atgggcatac aagttgtatt ttccaggagt cctgcaagga taatctggtt
     2401 gttgaaagta atggagacat ttacgaatgc gaccattttg tctatccaca gtacaaaatt
     2461 ggaaacatta ataaatctga actcaaaacg atgaacagtg tacaactgac agcgcaaaaa
     2521 aaacggattt cagcgaaatg tcagcaatgt gcatataaac ctatctgcaa tggcggttgt
     2581 cctaagcatc gtattactaa agtaaacaat gagactgttt cttatttttg cgaaggttat
     2641 aaaatccttt tttcaaccat ggtaccttat atgaacgcca tggttgagtt agctaagaac
     2701 agagtaccgc tttaccacat tatggatgtt gcaaaacaaa tggagaataa ttaataacgc
     2761 tctcttttaa ctccattctt agcctccttc ttatgacctt aacaaggcac ccgacataaa
     2821 ttcctgtcgg gtgtttcgtc atcatcatct tcccttgcga aaatcctggc ttacgtggta
     2881 tgcgtcaggg cattagcaat acccttgatt atcaatagca taaccataac cactgaaaat
     2941 taaaaatttc tatatatttc atattgttaa atgatgctta aataaatttc atcagtaaat
     3001 cattgccata gataatcatt ggatggcaca atcaattttc gctaatgata accattccca
     3061 ttacgttttg tatgttgata gcaaagtatc tctgtctgct gaagcccttt tggctgcgta
     3121 agaataataa aacttcagtg ttgttaatca ttattatcct cgcaatgatc cttggcgtgg
     3181 ttaaaattca ggtctggtta aacgactgga ataatgattt tttcaatgcg ctaagccaga
     3241 aagaaaccga taagctttgg cagctcgttc tttggttccc tgccctgctg gggatctttg
     3301 tattaatatc cgcaaacaaa acatggttaa ttaaactact gactattcgc tggcgcgaat
     3361 ggttaacaga ttactacctt aatcgctggt ttgcagataa aaactactac ttcacgcaga
     3421 tctacggcga gcataaaaat acagataacc ctgaccaacg tatcgctgaa gacattctct
     3481 tactgatcaa caaaacgctc agtctttcat ttggcttcat ccagtcactt agcatgctga
     3541 tcacctttac cgttattctt tggcaaagcg cgggtacgct ctcattcact gttggtggaa
     3601 cagaatggaa tatccagggg tatatggtct ataccgtcgt gctcattgtg atcggtggaa
     3661 ctttatttac ccataaggtg ggtaaacgta ttcgtccgct taatgtggaa aaacaacgta
     3721 gcgaagcgac atttcgaact aatcttgtgc agcataataa gcaggcagaa ctgattgcgc
     3781 taagcaatgc tgagtctcta caacgccagg agttgcgcga caattttcat accattaaag
     3841 agaactggca tcgtttaatg aatcgtcagc ggtggcttga ttactggcaa aatatttatt
     3901 cgcgctcgct tagcgttctt ccctactttc tgttattgcc gcagtttatt agcgggcaga
     3961 tcaatctggg cggactgatg aaatcgcgcc aggcatttat gctagtatcg aacaatttaa
     4021 gctggtttat ttataaatat gacgaacttg ctgaactggc tgcggttatc gatcgcttgt
     4081 atgagttcca tcaactcact gaatagcgcc ctacgaataa gcctaaaaat tgccaacatg
     4141 cggtacaagt ggctaatgcg agtattcgta cgcctgataa taagatcata ttagagaact
     4201 tgaactttca tgtttcgcca ggcaaatggc tattactgaa aggctactct ggtgcgggaa
     4261 aaaccacact gcttaaaaca ttatcccact gctggccgtg gtttaaaggt gatatttctt
     4321 ctcctgctga cagttggtat gtgtcacaaa caccgttaat caaaagcggc ttactgaaag
     4381 agattatttg taaagcactt cccctgcccg tagacgataa atcgttgagc gaagtactgc
     4441 atcaggttgg tctgggaaaa ttggctgcgc gtattcatga ccacgatcgc tggggagata
     4501 ttctttccag cggcgaaaaa caacgtatcg ccctggcacg attaatttta cgacgtccga
     4561 aatggatatt tcttgacgaa actacctctc atcttgagga acaagaggct atacgcttac
     4621 tgcgtttagt gcgtgaaaaa ctacccacaa gcggcgtcat tatggttaca catcaacccg
     4681 gtgtctggaa cctggctgat gatatttgtg acattagcac ggttttataa caacaataaa
     4741 agccctgctg tacggagata taaataaatg aagcgagttc ttattcctgg cgtcatttta
     4801 tgtggcgctg atgtggcgca ggccgtcgat gacaaaaaca tgtacatgta tttttttgaa
     4861 gagatgacgg tctatgctcc tgtccctgta cccgtaaacg gcaacacgca ttacaccagt
     4921 gaaagcatcg agcgtttacc gaccgggaat ggcaatatca gcgatctgct gagaaccaac
     4981 cctgcggtac gcatggattc aacgcaaagt acctcgttga accagggaga tattcgccct
     5041 gagaaaatct ctattcacgg tgcgtcgccc taccagaatg cctatttgat tgacggtatt
     5101 agtgcaacta ataacctgaa cccagcgaat gagtccgatg ccagtagtgc aaccaatatt
     5161 agcgggatgt cacaggggta ttatcttgat gtcagcttac tggacaatgt gacgctttat
     5221 gacagttttg tgccggttga atttggtcgc ttcaatggcg gggtaattga tgcaaagatc
     5281 aaacgcttca acgctgatga tagcaaggtg aaattgggtt atcgcactac gcgtttggac
     5341 tggttaacat cgcatatcga tgagaataac aagagcgcat ttaatcaagg ttcttcagga
     5401 agtacctatt tctctccaga ttttaaaaag aacttttata ccttgtcgtt taatcaggag
     5461 ctggctgata actttggcgt taccgccggt ttatcgcgcc gccagtctga tatcacccgc
     5521 gcggattatg tttcgaatga cggcattgtc gccggtcggg cacagtataa aaacgttatc
     5581 gatactgcat tgagcaaatt tacctggttt gccagcgacc gctttaccca cgatttaacc
     5641 ttaaaatata ccggctccag ccgtgattat aataccagca ccttcccgca gtctgatcgc
     5701 gaaatgggta ataaatccta tggtctggca tgggatatgg atacgcaact cgcatgggcc
     5761 aaactacgta ccaccgttgg ttgggatcat attagtgatt atacccgtca cgatcatgac
     5821 atctggtaca ccgaactttc atgtacatat ggtgatatta cagggcgttg tacccgtggc
     5881 ggattaggac acatttccca ggctgtagat aattacacct tcaaaacacg cctggactgg
     5941 caaaaattcg ccgtgggtga tgtttcgcat caaccctact tcggcgcgga atacatctat
     6001 tccgatgcat ggactgaacg ccataaccag tctgaatcct atgtgattaa tgctgcagga
     6061 aagaaaacta accataccat ttaccataaa ggtaaaggca gcctgggaat tgacaactac
     6121 acgctgtata tggcggatca cattagctgg cggaatgtgt cgttaatgcc cggtgtgcgt
     6181 tatgactatg acaactatct gtcaaaccac aatatctccc cgcgctttat gacggaatgg
     6241 gatatttttg ctgatcaaac ctcaatgatt accgccggtt ataaccgtta ctatggcggg
     6301 aatattcttg atatgggatt acgtgatatc cgcaatagct ggacggaatc ggtatcaggt
     6361 aataaaaccc tgacgcgtta tcagaatttg aaaacgcctt ataacgatga actggcaatg
     6421 ggattgcagc aaaaaatcga taagaacgtt attgcacgcg caagtgaagc gcatgatcaa
     6481 atcagcaaaa gcagtcgtac cgacagcgcg actaaaacca ccattactga atataacaac
     6541 gatggcaaaa ccaaaacgca ttcgtttaac ctcagttttg aactggccga acccctgcat
     6601 atccgccagg tagatattaa cccgcaaatt gtctttagct atatcaagag caagggcaac
     6661 ttgtcgttaa acaatggtta tgaggagagc aataccggtg ataaccaggt ggtttataac
     6721 ggtaatctgg tctcttacga tagcgttcca gtggcagatt ttaataaccc attaaagatc
     6781 tccttaaaca tggatttcac gcatcaaccg agcgggttag tgtgggcgaa tacgctggcc
     6841 tggcaagaag cgcgtaaagc tcgcattatc cttggtaaga caaatgcgca atacatcagc
     6901 gaatattcag attacaagca gtatgttgac gaaaaactgg atagcagcct gacctgggac
     6961 acccgcttgt cctggacgcc acaatttctg aaacaacaaa acctgacgat cagtgccgat
     7021 attctcaatg tactggatag caaaaccgct gttgatacaa cgaacaccgg tgtggcgacc
     7081 tacgccagtg gccgtacttt ctggcttgat gtcagcatga aattttaata gttcggattt
     7141 tacttaaccg ggtaaattcg cccggttttc gcatggagat aattatgaga aacctctgtt
     7201 tcttactgac gttagtggca actctgttgc tccccgggcg gctgattgcc gccgccttac
     7261 cgcaggatga aaagttaatt accgggcaac tggacaatga cttgcgatat atgatttatc
     7321 cgcatgctca tccaaaggat caggtaaatt tatggctgca aattcatacc ggttcattg
//
LOCUS       DAZXUL010000174         7193 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZXUL010000174 DAZXUL010000000
VERSION     DAZXUL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.174"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..249
                     /locus_tag="ACH3L1_003914"
     CDS             <1..249
                     /locus_tag="ACH3L1_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFT4078449.1"
                     /translation="VEIDNNIGENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTC
                     KQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            258..977
                     /locus_tag="ACH3L1_003915"
                     /pseudo
     CDS             258..977
                     /locus_tag="ACH3L1_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1316..2086)
                     /locus_tag="ACH3L1_003916"
     CDS             complement(1316..2086)
                     /locus_tag="ACH3L1_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078450.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRVARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(2131..2617)
                     /locus_tag="ACH3L1_003917"
                     /pseudo
     CDS             complement(2131..2617)
                     /locus_tag="ACH3L1_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2577..2798)
                     /locus_tag="ACH3L1_003918"
     CDS             complement(2577..2798)
                     /locus_tag="ACH3L1_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078451.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2799..3482)
                     /locus_tag="ACH3L1_003919"
     CDS             complement(2799..3482)
                     /locus_tag="ACH3L1_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFT4078452.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3546..3857)
                     /locus_tag="ACH3L1_003920"
     CDS             complement(3546..3857)
                     /locus_tag="ACH3L1_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078453.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3854..4756)
                     /locus_tag="ACH3L1_003921"
     CDS             complement(3854..4756)
                     /locus_tag="ACH3L1_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFT4078454.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            5196..6155
                     /locus_tag="ACH3L1_003922"
     CDS             5196..6155
                     /locus_tag="ACH3L1_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFT4078455.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            6155..6550
                     /locus_tag="ACH3L1_003923"
     CDS             6155..6550
                     /locus_tag="ACH3L1_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFT4078456.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6733..7113
                     /locus_tag="ACH3L1_003924"
     CDS             6733..7113
                     /locus_tag="ACH3L1_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFT4078457.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1967 a         1848 c         1755 g         1623 t
ORIGIN      
        1 gtggaaatag acaacaacat cggtgaaaac gcgttacgat cggtggcggt tggaagaaaa
       61 aattatctct ttttcggctc agacaaggga ggagaaagtg cggcgatcat ctacagtctg
      121 ctggtcacct gcaaacagaa cgaagtggag ccggaggact ggttgcgcga agtgatcgag
      181 aagctcaatg actggccgtc gaaccaagtg catgaactgc tgccctggaa cttctcgtct
      241 gtaaaataat ccttacgcta cgtacttctc ggggcgctta cgttgatgca gaagccatat
      301 gtgaagcggc ttcacgacca tcaatgcgtt ttgtccagcc aagaactgag acccagcaag
      361 caataagggc attacaccgg gtcagagaat cgctcatcag agataaggtg aaaacaacca
      421 accagatcca cggtttcctg ctggaatttg gaatcagcct gccgacaggg gatgccgtaa
      481 taaaacggtt gtctctcgta ttggctgaac acgaaatccc cgaatatctg agccgccttt
      541 tggtgaggtt gcatacccac tacctttacc ttgttgagca aatagcagag ctggaatcag
      601 aattaagtca gtcaatcaac gctgatgata ccgctcagcg cattatgaca attcctggtg
      661 ttgggccgat tactgccagc ttactatcgt cgcaacttgg tgacgggaaa cagttttcat
      721 gcagcagaga ttttgcggct tccacagggc tcgttccccg gcagtatagc actggaggaa
      781 aatcgactct gttgggtatc agtaagcgag gtgataaaaa tttacgccgg ttactggttc
      841 agtgtgcccg ctcattcatg atgcagcttg aacgccagca tggaaaactt gctgagtggg
      901 ttcgggagca actcaacaag aagcattcga atgtggttgc ctgcgctctg gcaaataaac
      961 tggcccgaat tgcctaggca atcactactc agcaaaaaga atatcaggcc tgatatatag
     1021 atgctgacaa aactcaatta gcttattgca aacaataaga tacaagttac cgtctggttt
     1081 tgcgaagatt gattattgat gacatgaacg gcaaatcgac ctgattaaaa acctaacagg
     1141 ggaaaatggc tcactgaagc cgaggggatt tttaggatta tcaggtgcgg aactcatcga
     1201 ggcgcgggca tcccttgccc acagagacgc cggatagatt taagcaagcc caccattaat
     1261 caaaaatcag tgttgcaaaa acgggggtga ccatagattt tccccgccat cgtcatcatt
     1321 tccggatttt tgccagggca tccgccatgc tgcggatttc ggcccgggct tcccggacaa
     1381 cttcccgcag aacaccacgg tcaaaccacc ggcggggcgg ttcatccggt atacgcagca
     1441 catcgcaggc cagcccactg gacaggatat gctcaataca atcgctgacc agatacattt
     1501 ccaccgccga aaaacgtgcg acccggttca ggatggcatc gagcaccacg ggcaggtttt
     1561 catcgtcttc atcgagccag caggggtgat cgctttcctt cacgaccagg cgaaagcggc
     1621 gcgtctggcg ggtcagcaac gtctgaccgc ttgtggtcag cgccgcccgg atacgccgcg
     1681 catcttctgc cccccggcaa ctgacactgc atactgcatc gcgcaccgca tcaagaactg
     1741 ccctgatttc gtcttcggtc agccattcac cggcatacgg gaaagacacc tgcgttccgt
     1801 ccgccggaag taccggcagc gactgccact gactgccgga cagaacaagt tccgctccgg
     1861 ttccggtcag tttctgaatg gcggaggcca ggtgcggcgg cagcacgcca tcgtggcggt
     1921 taacgagtaa cgtcgccagc cggggcgcat ccagccgcac gtaaatgctg atccagcccg
     1981 gatagttcgg gttacattgg tcaacatccg tctcttccgg ccagccctgc gccagcagca
     2041 ggttacgggc gtgacgacaa atcagagcat tcagcgtttc attcataatt catgaactcc
     2101 ctgtatcgcc gcccacaggc ggcgataaca tcagtcgttt tcgtgtgtgt tgtttttcag
     2161 caggctgcgg gcctgatcaa ccatcgcctg tcggcgggta acgtattccg ggagggggaa
     2221 accgtgatcc cgggcagccc attcacgttt ccacagtgcc tgttcagcga tctgagtcag
     2281 ggtctccagc agttcagcag ggacggtcac ctggcggttt tcgcgcacgt tgctgataat
     2341 ctccatcgcc gcggcggaag aaataccgga ctcaatccgc tagtcttccg ggatgtcctc
     2401 caggtgcgcc aggacggtcc tgatttcatg atcggacagg gaaatatcca ttccgtcagc
     2461 gagggcctga atctcttccg gggaccagat aaccagcatc agcggcgtgt ctcccgaata
     2521 ctttgctgcc agcgcccggc atagcgtttc gcatgttccg tacatactct ctcccctcaa
     2581 tctttgaagc aggaatccag ttccagaatt tcggtatatg cctccatcaa gaggcggttt
     2641 gcgcgaaaca acgtctgacg caccagacgt tccggcaggg tgtcagcatt aaagctgtac
     2701 cgccgctcca tctcattcat ggttgtacgc aggtcgcaca tggcattggc cacctccgcc
     2761 acttcggcgc gaagtttttc gtgtccggca tagttcattt acgcagcctc cggcataaac
     2821 cagtcatcat tcgcggcccc ctgctgcatg gcccgctgta cggcagccag tcgttgctgt
     2881 ccggcacggt gatactgctc aagcagctcg ataccgatat accggcgtcc ggactggagg
     2941 gcggcgacgc aggttgaacc gctgcctgca aacgggtcca gcactatcgc attcgggtgt
     3001 gtgaagctct caatcagcgg ttgcaggctg gtaacgggct tttccgtcgg gtgatggcgg
     3061 ttgcccgaat atttccagcc caacacgtcc ggcagcgggt tttgtggcag acgtggacgg
     3121 ccttttgcca ggatgtaggc gcattcgtgg cgatagccca catatgcggc cttcgatgtg
     3181 tagtttttgg tgaagaccag gtgaccaaca acgctgaatc ccgcattttt ccaggcagac
     3241 ataaagcgat cgacgcggtt ccagccatag aagctcacca tcagcgcgtc ttttttgagt
     3301 acgcggtaca tttcattaca ggccggttgc agccattcat cagttttatc accagcaatg
     3361 gtacgcccct gacggtcacg aaaaccgacg agatatggcg gatcggtgag gatgaaatca
     3421 atggcgttat caggaatacg ggccataaca tcgatgcagt taccgaggac aaaacgggac
     3481 ataacagatc cttctcttac agagggcacc cctgtggtgc ccgttccccg ctgcctgtgg
     3541 gctgttcatc gcttccggta acgggtcagg ccgaccgtgg aataccgcag ccgtcgtctt
     3601 tttgacagca aggttatgcc gcgaaagcgg cttgaagggc gttcatcaga aaagggcgac
     3661 gggctggcgt acactgtcac cgacaccagc cgggagcggt aaaagcgccc gtcccgtgcc
     3721 cgccggacgc gggacactga ccacaggcag cgtttatgcc gcgccgcgtc agccggcgca
     3781 gtaaaaagag gagcacggcg acatcctttg tcggaaaaga aacgggcggt tgcccgcccg
     3841 tctccgtcat acttcatccg ccataatgcg ccacattgtc gactatccac gcaggtccgg
     3901 tgacttccgg ctgctcatcg tcatctgtcg gggaagacca ttcaagttcc ccccacagca
     3961 cattgacgag ctctccacgc tcatacaact gccagccaca gaaatcacag ccctgttcgg
     4021 cataccagtg ctccagcgtg cagctgaaac ggcggcttaa ttctgcaata acgtcgcttt
     4081 ccggctgaca ccacggagtg tcgaaatcca cctgaatgaa gttttctccc tgactgctga
     4141 tattcaggtc atatccacaa ggccacttca cgccataccg ttccgtatac cagtgatatg
     4201 cagaaggaac accgttcaga acaccaccat taaagccgtt cacttcaaca tccaggcggg
     4261 tcggcagaac cgtcagcagg tcaaacggca tggttttttc cggcactacg ttgtcacaca
     4321 gacggttcca ccacaccgat acatcctcgt taccccatat gtcacaccag tcacctcttt
     4381 ttgcggtgaa atgcacagtg atggtttccc tggcatcatc cggtaatcct tcccaacgac
     4441 gctggccagt accgctctgt aaccagagtt catgcagcat caggcagttt tgttcatcca
     4501 gcaacacacc gttctgcagg tgtgtcagcc agcgggtgaa cgcgatattt tccgtcgata
     4561 cagcaccacg tccggcagcc gtcactccgg ggcactgttc agagcggata ttttcagtga
     4621 tctgcagaag tccggcactt ccggccagaa acagctgaat accttcattt gtggcgcggc
     4681 gataaagcgg tgtgactgca ccgctggcca gtcgtttaat ctcagcgatc tgtgccggct
     4741 caccaggaaa atacatacga ttcgagcacc agtttggcat gggaattctc ctctcacatg
     4801 cggcctccat caggccgtgc cgcacctgta accgggcgac cgcatacgaa tgcaggtgcg
     4861 gccccgaagc cgcagcgcgt ggaggaggaa tgacgaaacg tgaccgggtg aggaggccgg
     4921 cgacagcgac acacttgcat cggatgcagc ccggttaacg tgccggcacg gcctggttaa
     4981 atagtatccg tctcagttcg tggcgcaaaa tgagaaacca ataagaactc aaaatgaacc
     5041 acaacaagaa cataaagaga ctctaaaaga gcaacaccgt ttgacataga ctcaaaatga
     5101 gtcacaataa gaaacaataa aaaactcaaa aagatgcacc gacataaagg atgctcaatt
     5161 tgagataaac acaaactcaa aacgaggcaa aaagaatgct aaaagtatca tgcgatgatg
     5221 gttcaactaa cgtaaaactg gcctggcttg aagatggcga ggtaagaacc agtctgtcag
     5281 gaaacagctt taaagaaggc tggaacccgg gactgtttaa tgccggcaaa gtttacaatt
     5341 acgtcgtgga tgaaaaaaaa tacacctatg atttgggaag cactgctgtc ataggtacaa
     5401 cacatgtcag ttatcaatac agcaccacca atctgcttgc catacatcat gcactgctga
     5461 catccggcct tcagccacag gatgtagaac tgacagtaac attacctgtg accgaattct
     5521 ttgataatga caatcaacca aatgaagaaa gaatagaaag aaaaaaggca aatgttctcc
     5581 gtgagatatc tctgaataaa ggggaaacat ttaaaattaa aaaagttaat gtaatgcctg
     5641 aatctttgcc agctgcattt gaatcactaa aaaaagataa agtaaacaaa cttgaacgca
     5701 gcctgattat cgatcttgga ggaaccactc tggattgtgg tttaattctc ggtgcatttg
     5761 aaggcatttc tgaaatccgt ggctattcag aaatcgggac atcccgcatc actcataccg
     5821 tcatgaatgc gctgacgaaa gcctccacac catgtaatta ttttattgct gatgagttaa
     5881 taaaaaacag acacgataat gaatatctcc agacattaat aaatgatgta gctgaaataa
     5941 aaaacatttc tcatgtaata gacagagaag tgaaatctct ggcagaaagc atcaggcagg
     6001 aaatttcaac attctcagga atgaaccgaa tctatctcac tggcggagga gccgagctta
     6061 tttaccctca cataaagcaa tatttcccga atctgaaagt gaacaaggtg gatgaacctc
     6121 agtttgcatt ggtgaaagca atggtgcatg cgtaatggaa agcagtgatc caaaaaaaag
     6181 aaagaaagtt gtggcatacc tgcacccggc actttatccg caggacaacc tgacacaaca
     6241 aacaatagac tctctcccag tacaaatgcg gggagatttc tacagacaat cccttatttg
     6301 cggagcagct ctttattctg ttgccccccg actgttaacg ctaatcagcg tttttttcag
     6361 tgagaaaatc actgcagaaa atctggtgaa acttatagaa caaacaacag gatacacatc
     6421 cacatcaatt gatataagtg tgttaaaaaa catcatagag gcatcttcag aaaacaaatc
     6481 agagagcatc acttcaaaag atgatttcga agaacaaacc agacgcaatc tttctatgtt
     6541 aaaaaaataa taaaagaaga gcaggttcag tcaattacct gctcttcttt tatacatact
     6601 aaaaaatcag atttttacga atattcagat tatcataaaa aatgcaacag acaggaatgc
     6661 aataattttg cattatctcg ttgcttgtaa tgcaaatatg cattattgtc agtgtgtgac
     6721 aataacagag agatgacgat ggcgaccatt aacgcacgta ttgatgatga tataaagaac
     6781 caagcagatg aagtgctgaa gctgatgaat atcagtcaaa ctcaggctat tgcagctttt
     6841 tatcagtata ttactgaaca aaagaaactg ccatttgtga tcacctcaat tgtaaaaacg
     6901 ccgcatgatt tattacgtga aagtacagac atgttagctg aagctcttgc agtgatttcc
     6961 aatctacagg tgtggactga acagcaggat gggattggaa aagcaaagct aatggagtat
     7021 taccgccgcc tcgacgcact gtactgttgt gcaaaagaga aaataggtct tctttcagat
     7081 aaccgggatg ctgaactagg ctgtgtccct taatcatctg agttatagta actgtctgtt
     7141 tacacaacat attgaactat ggctcgctac gaccttcccg atgaggcatg gac
//
LOCUS       DAZXUL010000175         7159 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZXUL010000175 DAZXUL010000000
VERSION     DAZXUL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.175"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1076
                     /locus_tag="ACH3L1_003925"
     CDS             <1..1076
                     /locus_tag="ACH3L1_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HFT4078458.1"
                     /translation="AEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTA
                     STDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPV
                     NVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVA
                     GQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYV
                     SYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENI
                     SDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAIT
                     LHDAYKASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="ACH3L1_003926"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="ACH3L1_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFT4078459.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /gene="yoaI"
                     /locus_tag="ACH3L1_003927"
     CDS             complement(1623..1727)
                     /gene="yoaI"
                     /locus_tag="ACH3L1_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFT4078460.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="ACH3L1_003928"
     CDS             1897..2343
                     /locus_tag="ACH3L1_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFT4078461.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="ACH3L1_003929"
                     /pseudo
     CDS             complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="ACH3L1_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3215..4395
                     /gene="nimT"
                     /locus_tag="ACH3L1_003930"
                     /pseudo
     CDS             3215..4395
                     /gene="nimT"
                     /locus_tag="ACH3L1_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4450..4797
                     /locus_tag="ACH3L1_003931"
     CDS             4450..4797
                     /locus_tag="ACH3L1_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFT4078462.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="ACH3L1_003932"
     CDS             complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="ACH3L1_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFT4078463.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5256..6281
                     /gene="dgcP"
                     /locus_tag="ACH3L1_003933"
     CDS             5256..6281
                     /gene="dgcP"
                     /locus_tag="ACH3L1_003933"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFT4078464.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6314..6412
                     /locus_tag="ACH3L1_003934"
     CDS             6314..6412
                     /locus_tag="ACH3L1_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFT4078465.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6415..6489
                     /gene="yoaJ"
                     /locus_tag="ACH3L1_003935"
     CDS             6415..6489
                     /gene="yoaJ"
                     /locus_tag="ACH3L1_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFT4078466.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6650..7027)
                     /locus_tag="ACH3L1_003936"
                     /pseudo
     CDS             complement(6650..7027)
                     /locus_tag="ACH3L1_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(7021..>7159)
                     /locus_tag="ACH3L1_003937"
     CDS             complement(7021..>7159)
                     /locus_tag="ACH3L1_003937"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4078467.1"
                     /translation="AGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTS
                     C"
BASE COUNT         1727 a         1743 c         1744 g         1945 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttacctggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gaggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggt accgctctag
     1261 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcataaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccccaa gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc aggcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caatgccccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
     2881 acttgattgc tatgcttcac cccgcccggt atccaaaccg catattgtgg cggcaccatc
     2941 cacaaagcat tttccaccgt acaggtaatt gcaccatgta atgccagtat cagctgtcct
     3001 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
     3061 accgcggctt catggtgtcg gtgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctggcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcgcactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgccttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc ggcaagttct
     4201 gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt gctggcagcc
     4261 tgtgggccgc cgctaatggg taaaatacac gatgctaacg gtaactggtc cgtaccactg
     4321 atgggtgttg caatactttc actactgatg gcgattttcg gactttgcgc cgggagagac
     4381 aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcttgcgt gaaagggaga
     4441 acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt
     4501 tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga tttagccctt
     4561 gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt tcacggcgaa
     4621 gtcgtcgatt ttgcaacctt tcgcgagcaa tatcgcgcag aactggcgca acacgagcaa
     4681 gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct ctacgcagca
     4741 aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag cttgtgattt
     4801 tagtacggca tccggcggtt atttttcacc agccggatgg tcccgtcgcc acaatgccca
     4861 ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt gcgctgtctg
     4921 cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat ttgccatacc
     4981 aatctgcggc ggtttaggtg cttctggctg agaaggggtt gaacaaccag ccaggaccag
     5041 caagcaaggc agaacaaaac tgataatttt catttattga tctcacatat ttatccaagc
     5101 ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt cactcaaaac
     5161 atattacgtc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa agattaaata
     5221 atcagcactt acccgccgta tcctggagtt gttccgtgtc agatcagatt atcgcccgcg
     5281 tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag cttctggaga
     5341 tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc
     5401 gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta
     5461 ccgtcttgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg
     5521 atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atcaccacat
     5581 ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca
     5641 gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgccggac
     5701 tgattgcaca atatattcaa aaagaggcac tggttgaaca gctgcacgaa gccaatgctg
     5761 cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg cgggcgattt
     5821 ttgaaaatct gacgacgctg ttttccctcg cccggcatct taaccataag ataatgatcg
     5881 cgtttatcga tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg
     5941 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
     6001 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
     6061 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
     6121 ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
     6181 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
     6241 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
     6301 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
     6361 cgtattttta gcatggtttt ttattggctg ctatgctgcc ccgggagcat aaagatgaaa
     6421 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
     6481 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
     6541 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataacgg
     6601 ctgaagcggc gcacgaaaaa cgctttcgcg tttttcgtgc gccgcttcac ccctggttca
     6661 gcatttcgtc tgcagtctga accgcaggca accatttttc agcaagtctg cgctgggaat
     6721 cgctggcttt atcccatttg ctccacagtg cctcacgatc gtaatcgtgc gcttccagcg
     6781 tggggcgggc gatttcaaaa tacccctagc ttgtttctat ttgctcagat tttaataaca
     6841 actcagcacg tgctgccgct ggtagaactg atatatgaaa ttccaaagcc tttgaacccg
     6901 tacgcttgcg acgtagttct ggatgattgg ctgcaagctt ttcaagattg cggcgaatac
     6961 tcacctcatg tttaggaaag catgacattc ctacgcattc ttgcgctgtt acccaatggg
     7021 ttaacatgat gttttctcca tataccgaga aggccaaatc tgggctggtt ccatatccaa
     7081 atatctagca atgattgctt cgtatcgtgg tatatggcag cgaaatacgt tacgcatagt
     7141 gtcaggcttt aagcctgcc
//
LOCUS       DAZXUL010000176         7149 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZXUL010000176 DAZXUL010000000
VERSION     DAZXUL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.176"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..161)
                     /locus_tag="ACH3L1_003938"
     CDS             complement(<1..161)
                     /locus_tag="ACH3L1_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078468.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLT"
     gene            239..1333
                     /gene="ddlA"
                     /locus_tag="ACH3L1_003939"
     CDS             239..1333
                     /gene="ddlA"
                     /locus_tag="ACH3L1_003939"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFT4078469.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1357..1569)
                     /locus_tag="ACH3L1_003940"
     CDS             complement(1357..1569)
                     /locus_tag="ACH3L1_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFT4078470.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1829..2137
                     /locus_tag="ACH3L1_003941"
     CDS             1829..2137
                     /locus_tag="ACH3L1_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFT4078471.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2196..3290)
                     /gene="yaiW"
                     /locus_tag="ACH3L1_003942"
     CDS             complement(2196..3290)
                     /gene="yaiW"
                     /locus_tag="ACH3L1_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFT4078472.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3303..4523)
                     /gene="sbmA"
                     /locus_tag="ACH3L1_003943"
     CDS             complement(3303..4523)
                     /gene="sbmA"
                     /locus_tag="ACH3L1_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFT4078473.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4875..6032
                     /gene="ampH"
                     /locus_tag="ACH3L1_003944"
     CDS             4875..6032
                     /gene="ampH"
                     /locus_tag="ACH3L1_003944"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFT4078474.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6033..6536)
                     /locus_tag="ACH3L1_003945"
     CDS             complement(6033..6536)
                     /locus_tag="ACH3L1_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4078475.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6624..>7149)
                     /locus_tag="ACH3L1_003946"
     CDS             complement(6624..>7149)
                     /locus_tag="ACH3L1_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFT4078476.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         1815 a         1826 c         1727 g         1781 t
ORIGIN      
        1 gttaacttat aattaagaga aaaaaactca ccgcattgcc atttaagcca ggaataaatt
       61 agcttcccgt tcatcatttt tgtgatcttg cgcacatctt tgagataacg cactttcttc
      121 accagtacgt tgcacgcttt gtcagcagaa gattgcaaca atctttcaga aggccgcgaa
      181 tcccccgttt tgtcgtggta atgtatgcac tttgtgttag acgggatagg tttttaagat
      241 ggaaaaactg cgggtaggaa tcgtttttgg tggtaaatca gcggaacatg aagtgtctct
      301 gcaatcggca aaaaacattg tcgatgccat tgataaaagt cgcttcgacg ttgtgctgct
      361 gggcattgat aaacaagggc aatggcacgt cagcgatgcc agcaattatc tgctaaatgc
      421 agacgatcct gcccatattg cgttgcgccc ttcggcgacc agccttgcgc aggtgccagg
      481 taaacatgag catcagctta tcgacgcgca aaacggtcag ccgttgccga cggtggatgt
      541 cattttcccg attgtccacg gtacgctggg cgaagatggt tccttgcagg gaatgctgcg
      601 ggtcgccaat ttaccgtttg taggttctga tgttctggct tcagcagcct gtatggataa
      661 agatgtcacc aaacgtctgc tacgcgatgc cgggctgaac attgcgccat ttattaccct
      721 gacgcgcgct aatcgtcaca acatcagttt tgccgaagtg gagtctaaac tggggttacc
      781 actgtttgta aaaccggcta atcagggctc ttctgttggt gtcagcaaag taaccagtga
      841 agaacagtac gcaattgccg tcgatctggc gttcgagttc gatcataaag tgatcgttga
      901 gcaagggatc aaaggtcgtg agatcgaatg cgcagttctg ggcaacgaca atccgcaagc
      961 cagcacctgt ggcgagatcg tactcaccag cgatttctat gcctacgaca ccaagtacat
     1021 tgacgaagat ggcgcgaaag tggtagttcc ggcagccatt gcaccagaga tcaacgataa
     1081 gatccgggcg attgccgttc aggcttatca gacgttggga tgcgcaggca tggcgcgtgt
     1141 agacgtgttt ttaaccccag agaacgaagt ggtgatcaac gagatcaaca cactgcctgg
     1201 cttcaccaac atcagtatgt atccgaagct gtggcaagcc agcggtctgg gttacaccga
     1261 tctgatcaca cgtttgattg agctggcgct ggagcgtcac gctgcggata acgcattgaa
     1321 aaccacaatg taatatttta acgcccgata atattcttat tgggcgtttt cagtttcttc
     1381 atcgcgacga cgaataatca accccgccag ccagaaacta atcacccacg tcaccagacc
     1441 gacggcatag gtctgccaac cttttgcttc aaatcccagt acccccacca cgccattaag
     1501 aatgaatata aggccaatgg cgaacgcata gtagtgccag tcacggcgga tttttacagg
     1561 cagattcatg gcagctccgt taacgaaaaa caacatcctt gcatattctg taacaaccgt
     1621 ctgtggtgta gacaacattt tcgtaattat taataaattt aacttaaggt tgccattttg
     1681 tgactcacaa cgaagattca cgcttcggga atattcccaa catcaattta ccatgattct
     1741 gatattgaca atctaaatga cctgtcagac ttgccataag ccgctggtat cacaccagaa
     1801 gagaagcaga tgacttccgg aggtttttat ggctgatttc accctgtcaa aatcgctgtt
     1861 tagcggaaaa tatcgcaatg cctcttcaac gcctggcaac attgcctatg cgttgtttgt
     1921 gctgttttgc ttttgggctg gggcgcaatt gctgaacctg ttagtgcatg cgcccggcgt
     1981 ctatgagcgt ttaatgcagg tccaggaaac aggtcgccca cgggtggaaa ttggtttagg
     2041 tgtcggcacc attttcgggc tgatcccgtt tttagcaggc tgcctcattt ttgcagtggt
     2101 ggcgctatgg ctgcactggc gacatcgccg ccagtaaaat ttacgttgcc agatgcggcc
     2161 tgaaacgcct tatctggcct gacattcctg tttgatcact gtttcataca acgcgcccgc
     2221 cgttcgtcta cgcgcttcgc aaaccaggcc gtagtcaggt tgcgggtgat tttcgggctt
     2281 tccagttgaa tgccaggcaa catttcgcgg ggtagtgatt tacccgtttt cgcttcggca
     2341 agttgataaa ctttcttgta cagcgccgtc tcctcaaaag agaagctgtc acctttctct
     2401 aactgacggc gaatttcgct gtcgctcatc cccagttttc ccgccagttt gcgcgttgcc
     2461 agttcggttt tcccgggttc tttactgtcg taacgaatta aatcaccgtc cagtgccagc
     2521 ttcacgccgc tggccttact gactgcgttc tgaaacgcgg cattacggct ggcgtaccag
     2581 ccagcgttaa aatcagcaaa gcggtatatc ggtgcgctat agctggcggg ataattcagt
     2641 aaatggtaag taccaaacca caacccgccg cggcgactga agacttcctg acgaactgta
     2701 ccgtccattt tccacggata ccctttggta tgctgttcgg caaaagcaat gctgacctgc
     2761 atcggcccac cggtgcgcac cggattaagc gaaccaaaca gcgtctgccc cattggtacc
     2821 atgctgatca gatcgtcaaa aatcgcgctc aattgctttt ctgtacgcac cgaatctaac
     2881 cgttcgctat aacttttgcc gtttggcgat ttgattttta gcgcggtatg aaccagaaaa
     2941 gcaggaatgt gcatccgttc agcacgacgg tcaatctctt gccaggcgat cttgcttaag
     3001 cccggaacgg ccggatctgc ctgataattt gactcctgct gcgccaccgc cagcaccgag
     3061 tagacgtttt ccagcgtcgg tgccagcccc tgactttcaa aggtggtggc gagatctttc
     3121 gcccaggcat cgcggtcttt cacgctcgca ggcatcttct gccgcacgac gctcgctaca
     3181 tctatcgctt tttcgccctt tttcaacggt tgcggagcct gactactaca ccccgctaaa
     3241 accagaaccg ccaacaatga cagcgaagat agatgattga cgcgcgacat tacttctcct
     3301 ttttagctca aggtatgggt tacttcctga attttgtcac catccagctc atgttcaaag
     3361 ctgcgcagac gtttgtagat agacatcaac tcaaccagtg tggtccatga gttaatcagg
     3421 tactggaaag caccgcgaac ctgaccaaaa acgttggtaa tctgcgtcat caggccgagc
     3481 gtaatcgtac cggcaacaat tgacggaaac agcaagaaca aaccgaaaac gttatcgacc
     3541 tgcaaataga gaatgcgggc gatgttgaaa tacatatagt gaaaatagag gcgaaaatag
     3601 tttttccgta cggcgctaaa cagctcgcgt accgtaggcg gcgtcgcgcg cgtggcatcg
     3661 tcttcaccat aaaccagctc tttacggtag gcggcctcta ctcgctggtt tttaaactcc
     3721 agccccggca gtttgatccc taccactgcc agcaatccgg tccccatcag cgaccagacg
     3781 atagcggcaa tcaccagacc atacggaatg tgcccgataa tcggcagctc cggcacatgc
     3841 gcggagagcg ttaccagcac cggcaggaag gcgatcaacg tcatgatggc gttgataaaa
     3901 ctgacgccca tattctccag cgttgaagca aaacgcatgg tgtcttcctg cacacgctgt
     3961 gcggcacctt cgatatgacg cagttgttgc cagttcgcca tgtaatattc gttcatcgcc
     4021 gtacgccagc ggaacacgta gtgactgaca aagaagttgt tcagtacact gatcaccacc
     4081 gcgatcagcg caatccccag aaagacgccc acttcgcggt aaaattgttc gatagtgact
     4141 ttatgcggcg aacttagcgc ggtttgaatc agatcataga acggcgcata ccaggcgttg
     4201 acggcgaccc cgacttccac caaaaaccaa gtgacgaaga taatcagcgc agtaccgagt
     4261 attgaccagt attgccaacg gtgcgggctg tagataaacc agaaaaatgc aaaaagtcct
     4321 acgcaaacaa tgtagtaagc gtaaaaaatc aggaaatcca acgaccagaa acgcgcggcg
     4381 ctaatcggga tctgcccgga agcgccggtg atacgtgcca cccagtcacc cccaccggct
     4441 tgccagaaga taacggcaat caatgcccaa acaaaggccg agagaaaaaa cgttcccggc
     4501 tttgggaaaa aagacttaaa catatgcact cctgctaact tcttatcgtt ttaacaactg
     4561 ctgtgtatta accgcatgac cgctacaaag cgtcagccaa atgaaaccgc ccattgtgac
     4621 ccatagcaag gcgcttagtt cgccagcctg accataagaa ttgtttcgac ggatttcaca
     4681 aaccaaagat tgtttacata agcggattaa cacgcgcagg atcacaaatg caggcattgg
     4741 tgagcaggaa aaagatcgtg tttacgcgcc gttacgtttt taacactatt attgcactaa
     4801 ttcgcccttt gcaatccatc aatgagtagt ataaatacgc tcagttacct tcattcaatc
     4861 tatggacacc accgttgaaa cgtagtctgc ttttttctgc cgtgctgtgt gcggcgtcat
     4921 tgacctccgt ccatgcggcg cagccgatca ctgaaccgga gtttgcctct gatattgtcg
     4981 atcgttatgc cgatcatatt ttttacggca gcggtgccac ggggatggcg ctggttgtta
     5041 tcgacggtaa ccagcgcgtc tttcgtagtt atggtgaaac gcgacctggt aataacgttc
     5101 gcccgcagct ggattcggtc gtgcgtattg cttccctcac caaactaatg accagtgaaa
     5161 tgctggtgaa attgctcgac caggggaccg taaagttaaa cgatccgcta agtaaatatg
     5221 ctccgccagg cgcacgcgtg ccgacctaca atgggacgcc cattacccta gtgaatctgg
     5281 caacccatac cagcgccctg ccccgtgaac agcccggtgg cgcggcacat cgtccggtgt
     5341 ttgtctggcc aacgcgcgag caacgctgga aatacctttc tacggcgaag ctgaaagccg
     5401 cgccaggcag tcaggcggcg tactctaacc ttgcgttcga cctgctggca gatgcactgg
     5461 cgaatgcgtc cggcaagcct tatacccagt tgtttgaaga gcagattacc catccattgg
     5521 ggatgaaaga caccacctac accccctcac cggatcagtg ccgccgttta atggttgctg
     5581 agcgcggtgc cagtccgtgc aataacacgc tggcggcaat tggcagcggc ggcgtctatt
     5641 ccacgcctgg cgatatgatg cgctggatgc agcagtattt gtcatctgat ttttatcagc
     5701 gcagtaacca ggcagatcgc atgcagacgt tgatttatca gcgcgcgcag tttacgaaag
     5761 tgattggcat ggatgtcccc ggcaaagcgg atgcgcttgg tttaggctgg gtatatatgg
     5821 ccccgaaaga gggtcgtccg aggattattc agaagacagg cggtggtggc ggtttcatta
     5881 cctatatggc gatgatcccg cagaagaata ttggcgcgtt tgtggtggtc actcgttcgc
     5941 cgttgacgcg ctttaaaaat atgagcgatg gcatcaatga tctggtgacc gagctaagcg
     6001 ggaataaacc gttggttatc cccgcatcct gattaatact ctgaaggcaa acggttgata
     6061 gcgaccagtt tgcctttatt catttcgata tagccgcctt tacgcaaagc agcgagaact
     6121 tctgcgacga cagaacgcga tatgcgtgta cgttgatgga tataattcat cacaccaata
     6181 cgtgatcgca attcttcatt ccagtcaatc attgataata atgttgcgcg ggtttgtttg
     6241 gctggtagat gatatcccgt acaatttcct tctgatatta atttgtatgg tatatcgttt
     6301 ttcattaaac catcagccag ccccataata taaggagcct gggtaatacc gataagtacg
     6361 ttttcttctc tacgcagaga gataactcct tctaaaataa cgaaagtatc ttcgttattg
     6421 acatcactgg aaaatataac ttgcttttca ttattaaact cgaagcgcgt accgtatctg
     6481 gacaaacatt tatcgagctt accaaattcc tgaagaggtt taactacaga taacatttgc
     6541 gcgtcctttg cagtaatgcc cgtcaaatcc ttgacgggca ttatttagat taaattacca
     6601 gtatttcttc ggagtgaaga atattaccaa gtatatttaa cacccacgtt cgcggaccag
     6661 tcttgatcta cgtcaccacc accgaggtag ttagcatcgg tataggcgct gaagttcttg
     6721 gtgaagctga actgagtacc cagaccaaca cgtaccgcag acccttcagt accgttatcg
     6781 atggaatcac cgttcacatc gttatcgttg ttagagtcgt cgtagacgta agccagtttg
     6841 aagtacggag tcagagcctg atcttcgctg taggtgaagg tataacctgc atctacaccc
     6901 agttcataac gcatgctgtc gtaaggctga ccgtcaactt tcatgtcgtt gctcagctgg
     6961 tagtcatcac cagactggaa cagaccagaa atgctgccgt aaggagtcac gtaaccagca
     7021 tcacccagtt tgaagtcgta accggttttc aaaccgaagc cccaagcgtc ggagttggtg
     7081 ctaccgtcaa cgtaagtacc gttgctcatg gttgcagaca ggtcgttgtt gaagtgagag
     7141 tagctcaag
//
LOCUS       DAZXUL010000177         6938 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZXUL010000177 DAZXUL010000000
VERSION     DAZXUL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.177"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..815)
                     /locus_tag="ACH3L1_003947"
     CDS             complement(<1..815)
                     /locus_tag="ACH3L1_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFT4078477.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACH3L1_003948"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACH3L1_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACH3L1_003949"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACH3L1_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1398..1874)
                     /locus_tag="ACH3L1_003950"
     CDS             complement(1398..1874)
                     /locus_tag="ACH3L1_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFT4078478.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACH3L1_003951"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACH3L1_003951"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFT4078479.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="ACH3L1_003952"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="ACH3L1_003952"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFT4078480.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="ACH3L1_003953"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="ACH3L1_003953"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFT4078481.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="ACH3L1_003954"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="ACH3L1_003954"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFT4078482.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="ACH3L1_003955"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="ACH3L1_003955"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFT4078483.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1673 a         1741 c         1933 g         1591 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga atccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcga
//
LOCUS       DAZXUL010000178         6893 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZXUL010000178 DAZXUL010000000
VERSION     DAZXUL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.178"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="ACH3L1_003956"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="ACH3L1_003956"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFT4078484.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACH3L1_003957"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACH3L1_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFT4078485.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACH3L1_003958"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACH3L1_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFT4078486.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="ACH3L1_003959"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="ACH3L1_003959"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFT4078487.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="ACH3L1_003960"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="ACH3L1_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="ACH3L1_003961"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="ACH3L1_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFT4078488.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="ACH3L1_003962"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="ACH3L1_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFT4078489.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1943 a         1570 c         1448 g         1932 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcg
//
LOCUS       DAZXUL010000179         6807 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZXUL010000179 DAZXUL010000000
VERSION     DAZXUL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.179"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..742
                     /gene="yneK"
                     /locus_tag="ACH3L1_003963"
     CDS             <1..742
                     /gene="yneK"
                     /locus_tag="ACH3L1_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFT4078490.1"
                     /translation="IFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQFPMINLGAPI
                     KRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKD
                     PNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQESPHFQDLFL
                     FFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHE
                     DLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            892..2082
                     /gene="ydeA"
                     /locus_tag="ACH3L1_003964"
     CDS             892..2082
                     /gene="ydeA"
                     /locus_tag="ACH3L1_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFT4078491.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2107..2772)
                     /gene="marC"
                     /locus_tag="ACH3L1_003965"
     CDS             complement(2107..2772)
                     /gene="marC"
                     /locus_tag="ACH3L1_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFT4078492.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2984..3418
                     /gene="marR"
                     /locus_tag="ACH3L1_003966"
     CDS             2984..3418
                     /gene="marR"
                     /locus_tag="ACH3L1_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFT4078493.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3438..3821
                     /gene="marA"
                     /locus_tag="ACH3L1_003967"
     CDS             3438..3821
                     /gene="marA"
                     /locus_tag="ACH3L1_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFT4078494.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3853..4071
                     /gene="marB"
                     /locus_tag="ACH3L1_003968"
     CDS             3853..4071
                     /gene="marB"
                     /locus_tag="ACH3L1_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFT4078495.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4102..5001)
                     /gene="eamA"
                     /locus_tag="ACH3L1_003969"
     CDS             complement(4102..5001)
                     /gene="eamA"
                     /locus_tag="ACH3L1_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFT4078496.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5268..6383
                     /gene="ydeE"
                     /locus_tag="ACH3L1_003970"
                     /pseudo
     CDS             5268..6383
                     /gene="ydeE"
                     /locus_tag="ACH3L1_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            6510..6605
                     /gene="mgtS"
                     /locus_tag="ACH3L1_003971"
     CDS             6510..6605
                     /gene="mgtS"
                     /locus_tag="ACH3L1_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFT4078497.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            6605..6708
                     /gene="mgtT"
                     /locus_tag="ACH3L1_003972"
                     /pseudo
     CDS             6605..6708
                     /gene="mgtT"
                     /locus_tag="ACH3L1_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
BASE COUNT         1742 a         1679 c         1572 g         1814 t
ORIGIN      
        1 tattttttgc gctcccaacc caaagcctgt cactatgggg cgcgtatttc agaccggcgt
       61 cgagcgtgtg ctctttctgt ttatgaatga ttttattgaa caatttccaa tgatcaacct
      121 tggcgccccc atcaaaagag cgcatacacc acatattgaa cccctgcctc cggatcacca
      181 taccgctgca gattacttac gccagtttga tttgcttgtc ctgaatttta tctctcgcgg
      241 taattttgtc atactccccc gattatggaa taactctgag gttcacagat ggttcgtcaa
      301 taaagatcct aacttgatca ccgccattct cgacataacg gacagcgaat taaaagagga
      361 tttattgcaa agcctgatgg attcattagg ttctaacaaa catgtacaac ccgaagtctg
      421 catctgcttc ttatcccttt tagcagaaca agaatctcct cattttcagg acttgttttt
      481 atttttcgcc aatatgttat tgcactatca ccaatttatg aatcccaatg aaagtgatct
      541 gaatgacgtg ttgatgccag catcattaag tgatgataaa attatcaaac atatggcgcg
      601 caggaccctc aaactgtttg taaaaaatga aacgccgcca aaagttactc acgaagacct
      661 ggtgaaaaac aggcctcgct cccctgtcag accacctata cccgcaaccg ccaaaacgcc
      721 agacctccct gaacgtcatt aaaccgtgat gttaccgact ctctgactcg gaacagaatc
      781 agcgtcagag aaacggaaaa cgcgatccag atcacaaatg cattgtattc acatcattaa
      841 ccgttttaag atcatttcat cactttttcg caactcaccc gataatctgt tatgacaaca
      901 aacactgttt cccgcaaagt ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc
      961 atcttcaaca ccaccgaatt tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt
     1021 cacatgcaaa ccgctcaggt cggcatcatg ttgaccattt acgcatgggt agtagcgcta
     1081 atgtcattgc cttttatgtt aatgaccagt caggttgaac ggcgcaaatt actgatctgc
     1141 ctgtttgtgg tgtttattgc cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt
     1201 ctggtgatca gtcgcattgg tgtggctttt gcacatgcga ttttctggtc gattacggcg
     1261 tctctggcga tccgtatggc tccggccggg aagcgggcac aggccttgag tttaattgcc
     1321 accggtacag cactggcgat ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat
     1381 ttcggctggc gaatgacctt cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc
     1441 ctgattaagt tacttccctt actgcctagt gagcattccg gttcattgaa aagcctcccg
     1501 ctattgttcc gccgcccggc attgatgagc atttatttgt taactgtggt ggttgtcacc
     1561 gcccattaca cggcatacag ctatatcgag ccttttgtgc aaaacattgc gggattcagc
     1621 gccaactttg ccacggcatt actgttatta ctcggtggtg cgggcattat tggcagcgtg
     1681 attttcggta aactgggtaa tcaatatgcg tctgcgttgg taagtacggc gattgcgctg
     1741 ttgctggtgt gtctggcact gctgctacct gcggcgaaca gtgaaataca cctcggggtg
     1801 ctgagtattt tctgggggat cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg
     1861 ctggcgctgg caccagatgc taccgacgtc gcgatggcgc tattctccgg catatttaat
     1921 attggaatcg gggcgggtgc gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg
     1981 atgattggtt atgtgggcgc ggtgcctgct tttgctgcgt taatatggtc aatcattata
     2041 tttcgccgct ggccagtgac actcgaagaa cagacgcaat agttgaaagg cccattcggg
     2101 ccttttttaa tggtacgttt taatgatttc caggatgccg ttaataataa actgcacacc
     2161 catacatacc agcaggaacc ccatcaggcg ggagatcgct tcaatgccac ccttgcccac
     2221 cagccgcata attgcgccgg agctgcgtag gcttccccac aaaataaccg ccaccaggaa
     2281 aaagatcagc ggcggcgcaa ccatcagtac ccaatcagcg aaggttgaac tctgacgcac
     2341 tgtggacgcc gagctaataa tcatcgctat ggttcccgga ccggcggtac ttggcattgc
     2401 cagtggtaca aaggcaatat tggcactggg ttcatcttcc agctcttccg acttgctttt
     2461 cgcctccggt gaatcaatcg ctttctgttg cggaaaaagc atccgaaaac cgataaacgc
     2521 gacgattaag ccgcctgcaa ttcgcagacc gggaatcgaa atgccaaatg tatccatcac
     2581 cagttgccca gcgtaatacg ccaccatcat gatggcaaat acgtacaccg aggccatcaa
     2641 cgactgacga ttacgttcgg cactgttcat gttgcctgcc aggccaagaa atagcgcgac
     2701 agttgttaat gggttagcta acggcagcaa caccaccagc cccaggccaa ttgctttaaa
     2761 caaatctaac attggtggtt gttatcctgt gtatctgggt tatcagcgaa aagtataagg
     2821 ggtaaacaag gataaagtgt cactctttag ctaaccttgc atcgcattga ccaaaacttg
     2881 aaccgattta gcaaaacgtg gcatcggtca attcattcat ttgacttata cttgcctggg
     2941 caatattacc ccctacaact aattacttgc caaggcaact aatgtgaaaa gtaccagcga
     3001 tctgttcaat gaaattattc cattgggtcg cttaatccat atggttaatc agaagaaaga
     3061 tcgtctgctt aacgagtatc tgtctccgct ggatattacc gcggcacagt ttaaggtgct
     3121 ctgctctatc cgctgcgcgg cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt
     3181 cgacctggga gcactgaccc gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag
     3241 gttgccgaac ccgaatgata agcgcggcgt actggtaaaa cttaccacca gcggcgcggc
     3301 aatatgcgaa caatgccatc aattagttgg ccaggacctg catcaagaat taacaaaaaa
     3361 cctgacggcg gacgaagttg caacacttga gcatttgctt aagaaagtcc tgccgtaaac
     3421 aaaaaagagg tatgacgatg tccagacgca atactgacgc tattaccatt catagcattt
     3481 tggactggat cgaggacaac ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt
     3541 cgggttactc caaatggcac ctgcaacgga tgtttaaaaa agaaaccggt cattcattag
     3601 gccaatacat ccgcagccgt aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg
     3661 agccgatact ctatctggca gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa
     3721 ccttcaaaaa ttactttgat gttccgccgc ataaataccg gatgaccaat atgcagggcg
     3781 aatcgcgctt tttacatcca ttaaatcatt acaacagcta gttgataacg tgacaacgtc
     3841 actgaggcaa tcatgaaacc actttcatcc gcaatagcag ctgcgcttat tctcttttcc
     3901 gcgcagagcg ttgcggaaca aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg
     3961 gttgttcccc catcgcagga acaaccaccg tttgatttaa atcacatggg tactggcagt
     4021 gataagtcgg atgcgctcgg cgtgccctat tataatcaac acgctatgta gtttgttctg
     4081 gccccgacat ctcggggctt attaacttcc cacctttacc gctttacgcc accgcaagcc
     4141 aaatacattg atatacagcc cggtcataat gagcaccgca cctaaaaatt gcagacccgt
     4201 taagcgttca tccaacaata gtgccgcact tgccagtccg actacgggca ccagtaacga
     4261 taacggtgca acccgccagg tttcatagcg tcccagtaac gtcccccaga tcccataacc
     4321 aacaattgtc gccacaaacg ccagatacat cagcgacaag atggtggtca tatcaatagt
     4381 aaccagactg tgaatcatgg atgcggaacc atcgagaatc agcgagacaa caaagaaggg
     4441 aatgattggg attaaagcgc tccagattac cagcgacatc accgccggac gcgttgagtg
     4501 cgacatgatc tttttattga agatgttgcc acacgcccaa ctaaatgctg cagccagggt
     4561 caacataaag ccgagcatcg ccacatgctg accgttcaga ctatcttcga ttaacaccag
     4621 tacgccaaaa atcgctaagg cgatccccgc caattgtttg ccatgcagtc gctccccgaa
     4681 agtaaacgcg ccaagcacga tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag
     4741 tccagcaggc ataccaaagt taatggcgca gaacagaaag gcaaactgcg caaaactgat
     4801 ggttaatcca taccccaaga gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat
     4861 ggccggaaaa gcgaccagca taaagcgcaa accggccagc atcagcggtg gcatgttatg
     4921 aagccccact ttgatgacca caaaatttag cccccatacg accactacca gtagcgccaa
     4981 caccccatct tttcgcgaca ttctaccgcc tctgaatttc atcttttgta agcaatcaac
     5041 ttagctgaat ttacttttct ttaacagttg attcgttagt cgccggttac gacggcatta
     5101 atgcgcaaat aagtcgctat acttcggatt tttgccatgc gtccgatggt taataacaac
     5161 gacgaggcaa gaagggcgct ggtagagcgt cgtagggata agttcatttg tctgtccggc
     5221 agtgcgtttc gttatgtttt attttagaga tgtaaagaaa tagcatggct acgctgccat
     5281 ttatgaccat ttacttgagt cgccagtaca gcctgagtgt cgatctaatc ggttatgcga
     5341 tgacaattgc gctcactatt ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata
     5401 agttcgacaa gaaacgctat atgttactgg caattaccgc cttcgccagc ggttttattg
     5461 ccattccttt agtgaataac gtgacgctgg ttgtgctctt ttttgccctc attaactgcg
     5521 cctattctgt ttttgctacc gtgctgaaag cctggtttgc cgacaatctt tcgtccacca
     5581 gcaaaacgaa aatcttctca atcaactaca ccatgctaaa cattggctgg accatcggtc
     5641 cgccgctcgg cacgctgttg gtgatgcaga gcatcaatct gcccttttgg ctggcagcta
     5701 tctgttccgc gtttcccatg cttttcattc aaatatgggt aaagcgcagc gagaaaatca
     5761 tcgccacgga aacaggcagt gtctggtcgc cgaaagtttt attacaagat aaagcactgt
     5821 tgtggtttac ctgctctggt tttctggctt cttttgtaag cggcgcattt gcttcatgca
     5881 tttcacaata tgtgatggtg attgctgatg gggattttgc cgaaaaggtg gtcgcggttg
     5941 ttcttccggt gaatgctgcc atggtggtta cgttgcaata ttccgtgggc cgccgactta
     6001 acccagctaa catccgcgcg ctgatgacag caggcaccct ctgtttcgtc atcggactgg
     6061 tcggttttat tttttccggc aacaacctgc tattgtgggg tatgtcagct gcggtattta
     6121 ctgtcggtga aatcatttat gcgccgggcg agtatatgtt gattgaccat attgcgccgc
     6181 caggaatgaa agccagctat ttttccgccc agtctttagg ctggcttggt gccgcgatta
     6241 acccattagt gagtggcgta gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct
     6301 tagcgttggt gatcattgct gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt
     6361 gggggcagcc cgcgctttgt tgatttaagt cgaacacaat aaaaatttaa ttcagccttc
     6421 gtttaggtta tctctgctaa tatctttctc attgagatga aaattaaggt aagcgaggaa
     6481 acacaccaca ccataaacgg aggcaaataa tgctgggtaa tatgaatgtt tttatggccg
     6541 tactgggaat aattttattt tctggttttc tggccgcgta tttcagccac aaatgggatg
     6601 actaatgaac ggagataatc cctcacctaa ccggcccctt gttacagttg tgtacaaggg
     6661 gcctgatttt tatgacggcg aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg
     6721 atagatttgt gttttgcttt tacgctaaca ggcattttcc tgcactgata acggatcgtt
     6781 gacacagtag catcagtttt ctcaatg
//
LOCUS       DAZXUL010000180         6677 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZXUL010000180 DAZXUL010000000
VERSION     DAZXUL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.180"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1299
                     /locus_tag="ACH3L1_003973"
                     /pseudo
     CDS             1..1299
                     /locus_tag="ACH3L1_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(2030..3019)
                     /locus_tag="ACH3L1_003974"
     CDS             complement(2030..3019)
                     /locus_tag="ACH3L1_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078498.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(3104..3595)
                     /locus_tag="ACH3L1_003975"
     CDS             complement(3104..3595)
                     /locus_tag="ACH3L1_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078499.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(3916..4416)
                     /locus_tag="ACH3L1_003976"
     CDS             complement(3916..4416)
                     /locus_tag="ACH3L1_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078500.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(4406..4921)
                     /locus_tag="ACH3L1_003977"
     CDS             complement(4406..4921)
                     /locus_tag="ACH3L1_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078501.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(5352..5720)
                     /locus_tag="ACH3L1_003978"
     CDS             complement(5352..5720)
                     /locus_tag="ACH3L1_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078502.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(6132..>6677)
                     /locus_tag="ACH3L1_003979"
     CDS             complement(6132..>6677)
                     /locus_tag="ACH3L1_003979"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFT4078503.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
BASE COUNT         1644 a         1710 c         1756 g         1567 t
ORIGIN      
        1 gtgcagcggc agcggtacaa caggccagcg aaaaagtgat ctggggttcc tgttccgtca
       61 actgtggtag ccgctgtgca cttcgtctac atgttaaaga taatgaagtg acctgggtgg
      121 aaactgacaa taccggcagc gatgagtacg gcaaccatca gggtacgcgc ctgtttgcgc
      181 ggtcgctcca tccgccggcg tattaatcat cccgatcgct tgaatgaccc aatgaaacgc
      241 gtgggtaaac gcggtgaagg caaattcgaa cggattagct gggatgaagc cctggatact
      301 atcgccagta gcctgaaaaa aaccgtcgaa caatatggca atgaagctgt atatattcag
      361 tactcttcgg ggattgttgg cggcaatatg acccgctctt cgccatcagc ctcggcggtc
      421 aaacgcctga tgaactgcta cggcggttca ctcaatcagt atggctccta cagcaccgcg
      481 caaatttcct gtgccatgcc ctacacctac ggcagtaatg atggcaacag caccacggat
      541 attgaaaaca gcaagctggt cgtgatgttt ggtaacaacc cggcagaaac ccgcatgagc
      601 ggtggtggca tcacttatct tcttgaaaaa gcgcgcgaga aatcgaacgc caaaatgatt
      661 gttatcgatc cgcgatatac cgatacggct gccggtcgtg aagaggaatg gctccctatt
      721 cgcccgggca ccgatgccgc gctggttgcg ggtattgcct gggtattgat taacgaaaat
      781 ctcgttgatc aaccttttct cgataaatac tgcgtcggct atgacgaaaa aaccttaccc
      841 gcagatgcac ccaaaaatgg tcactataaa gcctatattc ttggtgaagg tgacgataaa
      901 acagcaaaaa cgccgcagtg ggcttcgcaa attaccggta tcccggtaga ccgtatcatc
      961 aaactggcgc gtgaaattgg cacaacaaaa cccgcttata tctgctaggg ctgggggccg
     1021 caacgccagg caaacggcga gctgactgca cgcgctattg ctatgttacc tattttgacg
     1081 ggcaatgtcg gcatcagcgg cggaaatagt ggcgcgcgtg aatcgaccta taccattacg
     1141 atagaacgcc taccggtgct ggataatccg gtcaaaacgt caatctcctg cttcagctgg
     1201 acggatgcta tcgatcatgg cccgcaaatg acggcactcc gcgacggtgt ccggggcaaa
     1261 gataaactgg atgtgcccat taagttcatc tggaactaca aggacttgaa gcggaccctt
     1321 agtaggcctg aggtcctgca ttgagtcaag accttgtgtc attctttgtg ccagacttgt
     1381 gtcattagac gggatgtcta gacggctggt caggaccctc cggtggcctc ggtctggact
     1441 tgaggcgggc tatgggggaa gtctgctggc ttaatgtatt agatggtcat tcacattttt
     1501 ctgtcagaaa ccatcggtcc cactgtgagt acaaacctaa gcccccagaa gcccacccac
     1561 agccttagac agagtgtcga atacattcac accgagtcga ccggagagga ccatatagag
     1621 gcccacagag aggaacacga gtttatagac cagagtcagg agggacctta ggtcttcgga
     1681 agtcatggac ataaagcccc cccctatctt ctattgcact accgcactag acaactagta
     1741 caaaacaaag gcccagacca ttggtccaga ccgtagttag ttaatcacag tgacaagcca
     1801 ccacctgaag tcctgctgat attcagtcca gacttacctc tgctcatggt cttacctccc
     1861 tcggtctcct gaccttgggt atcgactaca gcttgctgag cgtcatctgg aggagctaca
     1921 ggaggagcta caggaggcgg gaccgaagcc ccaccttgag tcccaccacc acctgtcagg
     1981 cccttggcga tggacctgaa gcctttacta atgctcttac ccattggtct tacgcttcgg
     2041 tcttggtgaa cttcaggcgt ttgactacag gcaggacggc agtagctgag ccagtgccca
     2101 tcttgatggt cgtagcagct tcagcgacaa cgaacacttt accgaggatt tccaagtcgt
     2161 gggactgaga gtcgccatca cgtgaaagga cgaccgtaat attctcaccg tcgtgacgga
     2221 agcagccgaa gctcagggcc gtgaagcgtt tagccagcat gaatacaccc ttgtcttcac
     2281 cgttgtaata ggcgatgtct gtccccagca tatcgacgat agcgtcttca ccggagaagc
     2341 cttgcttagc agccagacgg gacagtgctt tcagttggct ctcatgagcg ataataccgt
     2401 agtcagacac ctcagaaccg aaagaagact ctgtgttctc atacagagcg tccatgatag
     2461 cgtctgcctt agcggtcagg ctaccagtca ggacaggaag ctctacggtc ttcagcagct
     2521 tagaggattc cagaacgtcg accatgcgag cgttgatggt acggaccaca ttctcacaga
     2581 tgttcatagc gtcctgctga agtgtgccct gagtgaatcc ggtggatgac ttggcgtccc
     2641 agagcttggt ctgctcagag atgagaatag cctgctcgat tggttcgacc acgatttctt
     2701 gtccagacgg aacggtcacg acttcaggtc gcttgccacc acggacagga acgaccacag
     2761 agcggtcagc cagagtgtga gcaatgattg gggtcggtaa gctgttacgg gtcggcagga
     2821 cttctacagg gaacgggatg ttctcttgag cgtctttaag tacaccgttc agcatagcct
     2881 taaccgtctt agctaactgg agggcataga cctgcgggtc agcgttagtc ggcagacctt
     2941 cattgacctg aatgttgtcg gtatcggtga tgcgtactgg ctcaatgcca aactggaaag
     3001 cgtctttaat cgtagtcata ataatcaatc tcctaaatta gtttaaatac tgtgagtcct
     3061 caaaactgag gagtccctaa catcgggtca gggactgtgt gttttacaag ctgtccatcc
     3121 agttaccgcc tgactcaagg tcagcgacaa tagcggcacg ggagcgacca gcggaatggg
     3181 tagcactgcg gaggctctca ccataggtcc ggcgttcagc tttgacctgt tcgcggcgct
     3241 gtagctcttt ctgccactct gcatcggtca tgttctcttc acgcatacgc tcagcctgaa
     3301 gtcgttgctc tttagcgata cgctcagctt cagctcgttt gttcatctgg cggcgaccat
     3361 aggcggtcat caagtcatta ctgaagaagt tgccaatggt cacgccagct tcagccagag
     3421 cttccagtaa ctccattgcc tcgcggctgg tagacggcag gcctgacagg tagcccggaa
     3481 tgagggtctt gatagcgagc gacagcggat agggctgacc gtccagactc acagtgtttt
     3541 cataaaggtt gatgtcggtg actgacagct tccagttgtt gttgttgaac atcataggta
     3601 attctcctct tgagagttga gactatcggt atcgcagacg cagtagcgcc tacgggtctc
     3661 cccgcatcgg tcatctcaaa aggtttaaaa ttaggtttgg tatggtcagg actgcttagc
     3721 aaaggctcgg cgtaactgat tgtctaaatc acggtccacc agcatagcta tcagctcgtc
     3781 tcgactgtag taagggcgct cagggtcttc gactccaagt ctcagcagag acagcagatg
     3841 acgtgtgggc aggccacggt aacgaagggt cgctttgccg ataggtgtac gagggtcaat
     3901 catagccatg attacttacc tccactcaca gcgaccagcg gtcacagctt gatagtctga
     3961 atactcagcc tgtgagactt cacgtccggc gatgtagagg cctgtgccat gacgctggat
     4021 gaattccttg gtctccggct tgatgtcctc agcagacgct acagcagact gcacggtggc
     4081 gctggaggtc atagacacga aggctcgggt tactgccagt ccgtacccaa ggtccaacca
     4141 ccgaccgaac tctatagcgg ctactgggtc agccagccac gaggtcccta agtgacccat
     4201 gttaatcttt ttaacaaacc cgttttctgg gttggttaga cttctaccag ccttggtcct
     4261 catgaagcga gcaacctcgg tactacggtt atcttctacc ttaccaatct gaatgcctac
     4321 ccggcgtatc tcatccagtc gaaacaatcc gtcctgactg gtgatgtcct gaccgaagat
     4381 atgaatggtg tattcctgct tattgtcaga agtcataaga ggcacctccc gccagagctt
     4441 cggcaaagga cttgaagcgc tgttcttggt cagctttcgc ctgaaggcgt tcggcatcag
     4501 gtgggaccag cttatcaaag gtgccgtttt cgtaatcttc agttaggacc tgaaggcggc
     4561 gggaccattt gctctccacc tgcatcatcg cgttagagat gtgcttcaag tcggcatcag
     4621 agcgtgacgc tatccaatcc atctcttctg agtcaccctc acaggtgtaa ggattgttga
     4681 aatagaagat gtcctgcagg ccacgcttca gctcttcctc ggtgtgtgtc tggaggtatg
     4741 actggatagg ctccgcgtac atctcccgga ttttctcata gtcccagtct tccgcctggg
     4801 cataccatcg ggataacttc agcaggtcta agttatagcg acagcaatcg acactggact
     4861 ctgcattggt cagcaggatg attaagaact ggcggttagt tactggtaat tcggtattca
     4921 tacaaagatg ctcctttggt aaagcacttt ctgtctccac atggagtccg caccattcag
     4981 acgcatccta aacggaccca tcagaaagtg cttaacgaaa gtgaatggtt aaatgttgac
     5041 ggtctggagt gaccatgttc tccatgtacg tataaaggtg cgaccgcatg tcaggacgag
     5101 ctgtaaggac tccagatggt gagcggtgat agacttaagc caatgtagtg agtagacgag
     5161 ctactaaccg agacgactaa agaacgctaa cgagctggag gtcgatacag gaggtctgtc
     5221 agaggcggtc acgggccttg attacttagg agagatactt aatgtcggtt gaccagatgc
     5281 tcgcatgttc tgacgcatca gactcagagg cggaggctgg tctccctata gtgatagttt
     5341 agggtccgta atcatgaacc agctttactg gcgtggtact ggacggattt actggcgctg
     5401 acgacggtag catagacgta gcgagcgtag cgagttgaaa ccataggttc acccaaggca
     5461 acacgcttac ggctcaggga ctcctgcaca gcgcttacag ttaatgtgtc gtgcgttagt
     5521 agttgggtca gaatcaagta tgggctgaca ggcttggtat tgcttgccat tgattcgttc
     5581 aggcgaccag caccagtaag gacatcatcc agccaaccac tcaggtcgcc ctgaccatcg
     5641 aatgacagca ggtccccgca gtgctgctgc aagtaggctt tggtgaacgt gttgtcgtga
     5701 gttgcgattg gtcgtgccat agagtatctc ctgtagttga attagttggt tcccctatag
     5761 tgataatcaa gtccatcact ggtgatgcat acagtagcgc gacaggatgt gactcacagg
     5821 ttgaaccgaa tgtcaaggac taaagacgcc tcaggaatta aggaagtata tataaagaga
     5881 gaccagaggc gactcacagt taaacaggaa tgtcaagagg agaccatagg aatttggagg
     5941 gactggagtt taggacgcag tttaaataag accacagaca gacttagagt cggacagccg
     6001 aaggcggacc agtgggccta tagtcaggag ggattatgat tattatcacc ctccctaact
     6061 tcagtcagac ttcagtgacc caaggtctca accatcaagc cgtatatggt ctccaccttg
     6121 ggtctcccta cttaaagtaa agctgtctta agcacatcag ccagcctacc cacagcgtgt
     6181 cctttcccgt acaggtcata cgtaattgac tgactgctgt gacctaagat gccttgagcg
     6241 tccaccagat tgactccacg ctctttcatt cgggtcgcca gccaatgtct cagactgtgg
     6301 agagagacat cctgagagtc tcctagggcc tcagtcagga cccttgagtt aaaccagcta
     6361 gagtaggcgc tcggagtcat cccgaagaga ggaccctcat caggctgcat gtctacccac
     6421 gacaggaagg acgtcaggtc aaacccataa gcgccatcgg ttaacggcac aagtcggact
     6481 gaatgtttgt tcttcacaga cttcccgtcc ccatcttcat tgatgtcgat acagaccagt
     6541 ccattgtcca aggttacaat gtcacgttta gtcaggtggc agacctcagc agaccgagcg
     6601 ccagtgacca cagacaccag agacgcccat ctacgggcct cagaggtcgt gtgagccgtg
     6661 tgtcgtgtga actgata
//
LOCUS       DAZXUL010000181         6567 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZXUL010000181 DAZXUL010000000
VERSION     DAZXUL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.181"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..843
                     /locus_tag="ACH3L1_003980"
     CDS             <1..843
                     /locus_tag="ACH3L1_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HFT4078504.1"
                     /translation="KAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIE
                     APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQL
                     GNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVKALKQHPRKNKTAINI
                     EYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQW
                     ERSQ"
     gene            825..1076
                     /locus_tag="ACH3L1_003981"
     CDS             825..1076
                     /locus_tag="ACH3L1_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078505.1"
                     /translation="MGEISVKTGNNARNGGKNSLNRLIRCVCGKKIGPDPRNFNQRVS
                     LGRNDLLIRTFPKQLITSKNYSLVLWRESRTRIIVKISA"
     gene            1134..1496
                     /locus_tag="ACH3L1_003982"
     CDS             1134..1496
                     /locus_tag="ACH3L1_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFT4078506.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            1499..2584
                     /locus_tag="ACH3L1_003983"
     CDS             1499..2584
                     /locus_tag="ACH3L1_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFT4078507.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            2655..3263
                     /locus_tag="ACH3L1_003984"
     CDS             2655..3263
                     /locus_tag="ACH3L1_003984"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFT4078508.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            3361..4752
                     /gene="selA"
                     /locus_tag="ACH3L1_003985"
     CDS             3361..4752
                     /gene="selA"
                     /locus_tag="ACH3L1_003985"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFT4078509.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            4749..>6567
                     /gene="selB"
                     /locus_tag="ACH3L1_003986"
     CDS             4749..>6567
                     /gene="selB"
                     /locus_tag="ACH3L1_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFT4078510.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR"
BASE COUNT         1525 a         1693 c         1880 g         1469 t
ORIGIN      
        1 aaggcgggca atggccgacg gccctatccg ctggagacca tgctgcgtat tcactgcatg
       61 cagcattggt acaacctgag cgacggtgcc atggaagatg ccctgtacga aatcgcctcc
      121 atgcgcctgt ttgcccgatt atccctggat agcgccctgc cggatcgcac caccatcatg
      181 aatttccgcc acctgctcga gcagcatcaa ctggcccgtc aattgttcaa gaccatcaat
      241 cgctggctgg ccgaagcagg cgtcatgatg acccaaggca ctttggtgga tgccaccatc
      301 attgaggcac ccagctctac caagaacaaa gagcagcaac gcgatccgga gatgcatcag
      361 accaagaaag gcaatcagtg gcactttggc atgaaggccc acattggtgt cgatgccaag
      421 agtggcctga cccacagcct agtcaccacc gcggccaacg agcatgacct caatcagctg
      481 ggtaatctgc ttcatggaga ggagcaattt gtctcagccg atgccggcta ccaaggagcg
      541 ccacagcgcg aggagctggc cgaggtggat gtggactggc tgatcgccga gcgtcccggc
      601 aaggtaaaag ccttgaagca gcatccgcgc aagaacaaaa cggccatcaa catcgaatac
      661 atgaaagcca gcatccgtgc cagggtggag cacccgtttc gcatcatcaa gcggcagttc
      721 ggcttcgtga aagccagata caaggggctg ctgaaaaacg ataaccaact ggcgatgtta
      781 ttcaccctgg ccaacctgtt tcgggtggac caaatgatac gtcagtggga gagatctcag
      841 taaaaaccgg aaataacgcc agaaatggtg gaaaaaatag cctaaatagg ctgattcgat
      901 gtgtttgcgg gaaaaaaatc ggcccagatc cgagaaattt taatcagcga gtcagcttgg
      961 gaagaaatga cctgcttatt cgcaccttcc ctaagcaact catcacctct aaaaattatt
     1021 ctttggtgtt atggcgagaa tcgcgaaccc ggattattgt caaaatatca gcctgactcc
     1081 cttctggctg ccagccaaaa aacaacactg ataaacgtga atccgaggca gatatgtttc
     1141 taaactattt cgcgttggga gtgctgatct tcgtctttct ggtgattttt tatggaatca
     1201 tcgcgataca tgacatccct tatctgattg ccaaaaagcg caatcacccc catgccgacg
     1261 ctattcatac ggcgggctgg gtgagcctgt ttactctgca tgttatctgg ccgtttctgt
     1321 ggatctgggc gacgctctat caaccggagc gtggctgggg tatgcagtca catgttgcgt
     1381 cgcaggagaa agcgactgaa ccggaaatcg ccgcactttc tgaccgaatt tcccggctgg
     1441 agcatcaact cgccgccgag aaaaagactg actattccac gttcccggag atctaattat
     1501 ggatctattg attgttttaa cttacgtggc gctggcgacg gcggcgctgg gaggcgtgtt
     1561 tctggtgagt ggtttgattt tgttgatgaa ctacaaccac ccttacactt ttaccgcgca
     1621 aaaggcagtg atagcgatcc ctatcacgcc acaggtgacg ggaattgtta ctgaagtcac
     1681 tgacaagaat aatcagctta ttcaaaaggg cgaggtgctt tttaagctcg acccggttcg
     1741 ttaccaggcg cgagttgaca gacttcaggc tgacctgatg acggcgacgc ataatataaa
     1801 gacgctgcgt gcgcagctca ctgaagcgca ggccaacacc acccaggttt cagcggagcg
     1861 cgaccgtctg tttaaaaatt atcaacgtta cctgaaaggc agccaggcgg cggtgaatcc
     1921 gttctcggaa cgtgacatcg acgatgcgcg gcaaaatttc ctcgcgcagg atgcgttagt
     1981 gaaaggctcg gtggcggagc aggcgcagat ccagagtcag ctcgacagta tggtcaacgg
     2041 cgagcaatcg cagattgtga gcttaagagc gcaacttact gaagcaaaat ataatcttga
     2101 gcagactgtc attcgcgcac caagcaatgg ctacgtcact caggtactga tccgcccagg
     2161 tacatacgca gctgccttgc cgctgcgtcc ggtgatggtc ttcatccccg agcaaaaacg
     2221 gcaaattgtc gcccaatttc ggcaaaactc gctgttacgt ctgaaacctg gcgatgatgc
     2281 ggaagtggtg tttaacgcgc tacctgggca ggtgttccac ggcaaattga ctagtatttt
     2341 acctgtcgtg ccaggcggtt cttatcaggc gcagggggta ttgcaatcat taacggtcgt
     2401 tcccggcacg gacggtgtgc tgggaaccat tgaactggac cctaacgatg atatcgatgc
     2461 cttacccgac ggcatctacg cccaggtggc ggtttactcc gaccatttca gccatgtttc
     2521 ggtgatgcgg aaagtgctgc taagaatgac cagttggatg cattatcttt atttggatca
     2581 ttgagactta tatcacctgt gttttgcgtg agcctgagcc atactgatat ttttcctcgc
     2641 acaaaggact cggcatgaaa ctcgtcggta gctacaccag cccgtttgta cgcaaacttt
     2701 ttattctgtt gttagaaaag ggcataactt tcgaatttat taatgaactg ccctataacg
     2761 cggataacgg cgtggcgcaa tttaacccgt tagggaaagt gccggtgctg gtgaccgaag
     2821 agggcgaatg ctggtttgat tcgccgatca tcgctgaata tattgagtta atgaatgtcg
     2881 ctccggcgat gttgccgcgc gatccgctgg agtcgttgcg ggtgcgcaaa attgaggcgc
     2941 tggcggatgg cattatggat gccgggctgg tatcggtgcg tgaacaggcg cgtccggcgg
     3001 cgcagcagtc tgaagatgaa ttgttacgcc agcgggagaa aatcaaccgc agtctggatg
     3061 tgctggaagg atatctggtc gatggcacac tcaaaaccga tacggtcaat ctggcgacta
     3121 tcgccattgc ctgtgctgtc ggatatctca atttccgccg cgttgcgcca ggttggtgtg
     3181 tcgaccgccc acacttagtc aaactggtcg aaaacctgtt tagccgcgaa agttttgctc
     3241 gcaccgaacc gccaaaggct tgatgcgcga tatgtcctcc tgacccatct cacgttacaa
     3301 tccgtggtta tgttaaacgc ccttctccgt gtgagagggc cttgatcagc caggtttcct
     3361 atgacaaccg aaacgcgttc cctctatagt caacttccgg ctattgatcg cttattgcgc
     3421 gatagctcct tcctttcttt gcgtgatact tatggtcaca cccgcgtggt ggaattgttg
     3481 cgtcagatgc tcgacgaagc gcgagaagtg attcgtggca gccagacgct gcctgcgtgg
     3541 tgtgaaaact gggcgcaaga agtcgatgcc cggttgacga aagaggcgca gagcgcgctg
     3601 cgtccggtga tcaacctgac gggaaccgtg ctgcatacca accttgggcg agctttacag
     3661 gcggaagccg cggtggaagc cgttgcgcag gctatgcgtt cgccagtgac cctcgagtac
     3721 gatctggacg acgccggacg cggacatcgc gatcgggcgc tggcgcagct gctgtgccgt
     3781 attacggggg cggaagatgc ctgtatcgtc aataacaatg cggcggcggt gttattgatg
     3841 ttggcggcca ctgccagcgg gaaagaggtg gtggtatctc gcggcgaact ggtggagatt
     3901 ggcggcgcgt ttcgtattcc cgatgttatg cgtcaggcag gctgcaccct acacgaagta
     3961 gggaccacca accgcacgca cgcgaatgat tatcgtcagg cggtgaatga aaataccgca
     4021 ctgttgatga aagtacatac cagtaactac agcattcagg ggttcaccaa agcgatagac
     4081 gaagctgaac tggtggcgct cggcaaagag ctggatgttc cagtggtgac tgatttaggc
     4141 agtggctcgc tggtcgatct tagccagtac ggtttgccga aagagccaat gccgcaggag
     4201 ttgattgcgg cgggcgtcag tctggtgagc ttctccggcg acaagttgtt aggcgggccg
     4261 caggccggga ttatcgttgg taaaaaagag atgatcgccc gcctgcaaag ccacccgctg
     4321 aagcgtgcat tacgcgcgga taaaatgacc ctcgcggcgc tggaagccac gttgcgtctt
     4381 tatttacacc ctgaagctct gagtgaaaaa ttaccgaccc tgcgcctgct tacccgcagc
     4441 gcagaggtca ttcaaatcca ggcacaacgt ttacaggctc cccttgtcgc acattacggc
     4501 gcggagtttg cggtacaggt tatgccatgt ctttcgcaga ttggcagtgg ttcgctgccg
     4561 gttgatcgcc tgccgagcgc ggcattaacg tttacacccc atgatggacg cggtagccac
     4621 cttgagtcat tagccgcccg ctggcgtgaa ttgccagtgc cggtgattgg tcgtatttat
     4681 gacggacgat tgtggctgga tttacgctgc cttgaagatg agcaacggtt tttggagatg
     4741 ttgttgaaat gattattgcg actgccggac acgttgacca cggcaaaaca accttattgc
     4801 aggcgattac tggcgtaaat gctgaccgtc tgccggaaga aaaaaagcgc ggcatgacca
     4861 tcgatctcgg ctatgcctac tggccgcagc cggatggtcg cgtgcctggt tttatcgacg
     4921 ttcccggtca tgaaaagttt ctttccaaca tgctggcggg cgttggtggt atcgatcacg
     4981 cgctgttggt ggtggcgtgc gatgacggcg tgatggcaca gacccgtgag catctggcga
     5041 ttttgcagct gaccggtaac ccgatgctga cagtggcgct gaccaaagcc gatcgcgtgg
     5101 acgaagcgcg tgttgatgag gttgaacgcc aggtaaagga ggttctgcgg gaatacggtt
     5161 ttgctgaggc aaaactgttt atcaccgcag caaccgaagg tcggggaatt gatgccctgc
     5221 gcgagcatct gcttcagttg ccggaacgcg agcacgccag ccaacatagt ttccgcctcg
     5281 cgattgaccg cgcatttacc gtaaaaggtg ccgggctggt cgtcaccggt acggcgttaa
     5341 gcggggaagt gaaggtaggc gattcactct ggctgactgg tgtaaataaa ccgatgcgtg
     5401 tacgtgcgct gcatgcgcaa aaccagccaa cagaaaccgc caatgccggg cagcgtatcg
     5461 cgcttgacat cgcgggtgat gcggaaaaag agcagattaa ccgtggcgac tggctgcttg
     5521 ccgatgtgcc gccagagccg ttcacacggg tgattgtcga gcttcaaacc catgcaccgc
     5581 tgacccagtg gcagccgctg catattcacc acgccgccag ccacgtcacg ggacgcgttt
     5641 cactgctgga agataacctt gctgaactgg tcttcgacac cccgttatgg ctggcagata
     5701 acgaccgcct ggtattgcgc gatatctctg cccgcaacac gctggccgga gcgcgcgtcg
     5761 tgatgcttaa cccgccgcgt cgcggtaaac gtaaaccaga gtatctgcaa tggctggctt
     5821 ccctcgccag ggcacagaat gatagcgaag cgttagccgt tcacctgcaa cgcggcgcgg
     5881 ttaatcttcc tgatttcgct tgggcgcgcc agctcaacgg cgaagggatg cgcgaactgc
     5941 tgcaacagcc tggctatatt caggctggtt atagcttgtt gaatgcgccg gttgccgctc
     6001 gctggcaacg gaaaattctc gacacattag cgacttatca tgagcaacat cgcgatgaac
     6061 ctgggcctgg gcgcgaacgt ctgcgacgta tggcgttgcc aatggaagat gaagcgctag
     6121 tactgttgct gattgaaaag atgcgcgaaa gcggcgacat cctcagtcat cacggttggc
     6181 tgcatctgcc ggatcacaaa gcgggcttca gcgaagagca gcaggccatc tggcaaaaag
     6241 cagagccgct gtttggtgac gagccgtggt gggtgcgtga tctggcaaaa gagacgggta
     6301 ccgacgagca ggcaatgcgc ctgacgctac gccaggcggc gcagcaagga ataattaccg
     6361 cgatcgtcaa agatcgttat taccgtaacg atcggattgt cgaatttgcc aatatgatcc
     6421 gcgatctcga tcaggagtgt ggttcaacct gcgcggcgga tttccgcgat cgcttaggcg
     6481 taggccgaaa gctggcaatt cagattctgg aatattttga ccgcattggc tttacgcgtc
     6541 gtcgtggaaa tgatcattta ttgcgcg
//
LOCUS       DAZXUL010000182         6520 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZXUL010000182 DAZXUL010000000
VERSION     DAZXUL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.182"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..115
                     /locus_tag="ACH3L1_003987"
     CDS             <1..115
                     /locus_tag="ACH3L1_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078511.1"
                     /translation="LDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="ACH3L1_003988"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="ACH3L1_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFT4078512.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="ACH3L1_003989"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="ACH3L1_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..>6520
                     /gene="narH"
                     /locus_tag="ACH3L1_003990"
     CDS             5646..>6520
                     /gene="narH"
                     /locus_tag="ACH3L1_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFT4078513.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1558 a         1713 c         1690 g         1559 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg actcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct
//
LOCUS       DAZXUL010000183         6461 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZXUL010000183 DAZXUL010000000
VERSION     DAZXUL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.183"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(139..993)
                     /locus_tag="ACH3L1_003991"
                     /pseudo
     CDS             complement(139..993)
                     /locus_tag="ACH3L1_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1101..1379
                     /locus_tag="ACH3L1_003992"
     CDS             1101..1379
                     /locus_tag="ACH3L1_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078514.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="ACH3L1_003993"
     CDS             complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="ACH3L1_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFT4078515.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="ACH3L1_003994"
     CDS             complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="ACH3L1_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFT4078516.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNCSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3533..3865
                     /gene="hdeA"
                     /locus_tag="ACH3L1_003995"
     CDS             3533..3865
                     /gene="hdeA"
                     /locus_tag="ACH3L1_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFT4078517.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3981..4307
                     /gene="hdeB"
                     /locus_tag="ACH3L1_003996"
     CDS             3981..4307
                     /gene="hdeB"
                     /locus_tag="ACH3L1_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFT4078518.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4371..5017
                     /locus_tag="ACH3L1_003997"
                     /pseudo
     CDS             4371..5017
                     /locus_tag="ACH3L1_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(5059..5589)
                     /locus_tag="ACH3L1_003998"
     CDS             complement(5059..5589)
                     /locus_tag="ACH3L1_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFT4078519.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5745..6311)
                     /gene="slp"
                     /locus_tag="ACH3L1_003999"
     CDS             complement(5745..6311)
                     /gene="slp"
                     /locus_tag="ACH3L1_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFT4078520.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1872 a         1350 c         1385 g         1854 t
ORIGIN      
        1 tttatcgata tcgaagcatt gagcattttt aactcgtttt atcttgaatg caatcggcgc
       61 ataacttatt gaatcccatc aaatcccgtc tttttttacc cattcccggt tatcttgaat
      121 aaattaccta gtcataaata cattctggcg gctaccttca tccactaatg ccgtgacata
      181 catgccaggc agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc
      241 cgtggtttca tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt
      301 accattttcc agattgagct gtactggcgt actgccctga acctgtttga tttgcccact
      361 ggcgacctct tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat
      421 cgggtccaga cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc
      481 gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg
      541 cagattgatc gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc
      601 attcagctgg gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa
      661 cgatgcctgg cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag
      721 cgagcctttg gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga
      781 atcgccctgg ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac
      841 ctggggacgt atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac
      901 attgaccgaa ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt
      961 ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa
     1021 cagctttctt cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta
     1081 aattgcgcgc gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag
     1141 ccagtaaggt gggatacagg cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg
     1201 ttgccgttct gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat
     1261 acacatacca ggggaatcaa gtcgttttag cctctccttt agtaatcacc gggggtaatg
     1321 aaatcagcat ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac
     1381 taaaaataag atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg
     1441 tacataatgt tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg
     1501 atattgcttt catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa
     1561 aaacataatt gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt
     1621 ttgatacttt ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg
     1681 tgataaggtg ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct
     1741 tcttcatcaa ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct
     1801 acttttttga tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc
     1861 caaaagccct gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta
     1921 gccgttatcg tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg
     1981 ttcaatatag taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca
     2041 ctaatcaata cttcagattt atccatcacg cactccatat attgatccta agtaagattc
     2101 ctggttgtta tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca
     2161 agaataagtt atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt
     2221 aatccgattt aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc
     2281 tttcttttaa cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta
     2341 tatagcgtct ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg
     2401 aacccggatt acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact
     2461 ttcccacagc acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt
     2521 ttgaaatggc taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat
     2581 aatcactagc aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc
     2641 ggagagagta ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg
     2701 ggtaattatt gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat
     2761 atcagttcaa ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca
     2821 aggaatatcc aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct
     2881 ttcattgaac gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa
     2941 ccggcaataa atgccgcaat ggcaaaaatg cccagctccg gtgcacggat gaagaaatag
     3001 ccgatcaata aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga
     3061 ctgcagttgc tgaataaccc gacaataagc gcaatacccg agcagattaa taatgcaccc
     3121 actactgtgc ttaaaatatc gccagagacg aacgggaaac tgatacacag caacccgacg
     3181 ataaacagca gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga
     3241 tcaaacttca aaattgttgc cttatctata tataacatag aaccacccta taaaattaag
     3301 aagaaaatcc cctgctatca atctatgcca aaaacgcgtc taagaatgca gtcgatttaa
     3361 taaaaatttc ctaattgcag tatctgatgc atctgtaact cattgtattg aaataaaaat
     3421 atctgatttt gatattttcc atcaacatga catatacaga aaaccaggtt ataacctcag
     3481 tgtcgaaatt gattcgtgac ggctctttca ctttatagtt gaggatatta cgatgaaaaa
     3541 agtattaggc gttattcttg gtggtctgct tcttctgcca gttgtgagca atgcagcgga
     3601 tgcgcaaaaa gcagctgata acaaaaaacc ggtcaactca tggacctgtg aagatttcct
     3661 ggctgtggac gaatccttcc agccaactgc agttggtttt gctgaagcgc tgaacaacaa
     3721 agataaacca gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa ccccagctat
     3781 cgttcaggct tgtactcagg ataaacaagc caactttaaa gataaagtta aaggcgaatg
     3841 ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt acatcgtact tccttgcata
     3901 ttgaacaggc cggaatatct tctttaaaag cagctattcc tcctgttcat atataatctc
     3961 tatattgaat gggttacaaa atgaatattt catctctccg taaagcgttt atttttatgg
     4021 gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc gttggcagcc aatgaatctg
     4081 ctaaagatat gacctgccag gaatttattg atctgaatcc aaaagcaatg accccggttg
     4141 catggtggat gctgcatgaa gaaacagtat ataaaggtgg cgataccgtt actttaaatg
     4201 aaaccgatct cactcaaatt cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa
     4261 atttgtatac cttcaaaaat caagcatcta atgacttgcc gaattaatga ggtacaagta
     4321 aaaaggagta gcaagttgag ccatcttgct gctccttttt gcatttttat atgacagcag
     4381 aatttattat acgtcttata ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc
     4441 aaatgcgcgg caaaggagca gggttacgca cacatgtatt aattggcatg ggaagcgccc
     4501 tgtttatgat tgtttcgaaa tatggttttg ctgacgtgct gtctttagat cacgtcggac
     4561 tcgaccccag ccgtattgct gctcaggtgg tgacgggcgt cgggtttatc ggtgcaggta
     4621 acattctcgt tcgtaaccaa aatattgtag gtctgacgac ggcagcggat atctgggtga
     4681 ccgccgcata ggtatggtta ttggcagcgg tatgtacgaa ctgggtattt atggttcagt
     4741 gatgaccttg ttggtgctgg aagtcttcca tcaattaacc ttccgcctga tgaacaaaaa
     4801 ttaccattta cagctgacat tagtgaacgg taatacggta tcaatgctcg actggtttaa
     4861 gcagcaaaaa ataaaaactg atttagtttc gttgcaggaa aatgaagatc atgaagtggt
     4921 cgccattgat atccagcttc atgccacaac gttgatagaa gacctgttgc gattattaaa
     4981 aggtatggca ggggtgaaag gcgtttcaat tagctaatct gtgtggcagc gtagccagac
     5041 tcaccgtaag cctgaaattc acaccagata atcaatatgc tgatggcgta ccagctcatt
     5101 gatccgctta agattgagga tcatcatgat attgtgttta tggcagtaga acgtttttag
     5161 cgatattttc agtttctcca gaatatcttc agttgtcatt tgattcgcaa tgcagctcag
     5221 aacctgatgt tgcttgcgcg aaagtgacac attcgcttca ggcacagagc agtaactgcc
     5281 attgatgagt gcgacaaaat caatgatagt gatagtccgc ggaacaaagg tcaccggtac
     5341 tttccccaga cagcgtttaa tattaaaggg ggagaaaaca atgacatgga caggttttaa
     5401 tcgttcaatt tgagtgagaa aattagaatg aaatacatta ttcattaatg tatcaataat
     5461 gacgaaggca ttctgatgca acattacgtc gatctcttct tcgttctgta tatgaacgac
     5521 attaccttta ctcagaatgt ttttcatcgc ggtgaagaac atcgtatccc tggtaattat
     5581 aagaaacata accgactcct ggtgcggaca ttaactttat aatcagtttg ccaaaggtaa
     5641 ttaacaggac agggcttata ataataatag ttagcttact gtatattaat tttatgtaat
     5701 acatgatgca gatgctgaga aagcatctgc atctttcggt ggtgttattt gaccagctca
     5761 ggtgttacct gactcaccgc attggtgtag taaggcgcac cccagcccgg ttccggccag
     5821 aatgcaccat agccgtaatc ccacaggtta taggtggtat taaccacttc tctcaaatgc
     5881 cacacctgga tgccctgcat attcacttcc aggaagttat acgggacttt attgataaag
     5941 cctggttgtt caccctgaat ggtaccgagg atggtaacaa agtgattacg atagttcact
     6001 ggatcaagga agccgctttg tctggcgagc agtcggccct gatagttggc ttcaatatca
     6061 ggcttcgcat agctatccaa cggtaataca gcgatttcta acaacgtatc cgttttgcca
     6121 ttgataacgt tgataacctt cccaccaaag cgcgcttgtt gaccaacata taaccccggc
     6181 tggttatgaa cagcaacaaa acttttttga atatcaggtt ggttattgcc tttgatattt
     6241 tgcggaattg aactacatgc ggcaagcaaa aatgaaaggc tgaggatgag tgcacctttt
     6301 gtcatgttca tgttactatc cttatcaaca aatattaaaa ccataataga tatttcttta
     6361 tagttttcat ctgattctga ggtgatatgc ctgatagtaa cgtaaacctg tctgatgttt
     6421 atatcccttc tgtttagttc aattccgtta ttttggggcg t
//
LOCUS       DAZXUL010000184         6321 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZXUL010000184 DAZXUL010000000
VERSION     DAZXUL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.185"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..378
                     /locus_tag="ACH3L1_004000"
     CDS             <1..378
                     /locus_tag="ACH3L1_004000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078521.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACH3L1_004001"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACH3L1_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFT4078522.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACH3L1_004002"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACH3L1_004002"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFT4078523.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="ACH3L1_004003"
     CDS             complement(1787..2476)
                     /locus_tag="ACH3L1_004003"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFT4078524.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACH3L1_004004"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACH3L1_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFT4078525.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACH3L1_004005"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACH3L1_004005"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFT4078526.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACH3L1_004006"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACH3L1_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFT4078527.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1610 a         1709 c         1564 g         1438 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc t
//
LOCUS       DAZXUL010000185         6255 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZXUL010000185 DAZXUL010000000
VERSION     DAZXUL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.186"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACH3L1_004007"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACH3L1_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFT4078528.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACH3L1_004008"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACH3L1_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFT4078529.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACH3L1_004009"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACH3L1_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="ACH3L1_004010"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="ACH3L1_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1661 a         1455 c         1470 g         1669 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgttaa ttaacaaatg atatcttcta tatattttta
     6181 ttgtttatag atggctattt cggtaaaaat gcattttcag aatatcaacc agcggtaaat
     6241 cgaagtaaag ggagt
//
LOCUS       DAZXUL010000186         6091 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZXUL010000186 DAZXUL010000000
VERSION     DAZXUL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.187"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..84
                     /locus_tag="ACH3L1_004011"
     CDS             <1..84
                     /locus_tag="ACH3L1_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078530.1"
                     /translation="YESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACH3L1_004012"
                     /pseudo
     CDS             complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACH3L1_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(1611..2201)
                     /locus_tag="ACH3L1_004013"
     CDS             complement(1611..2201)
                     /locus_tag="ACH3L1_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFT4078531.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACH3L1_004014"
     CDS             complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACH3L1_004014"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFT4078532.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3179..3715
                     /locus_tag="ACH3L1_004015"
     CDS             3179..3715
                     /locus_tag="ACH3L1_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFT4078533.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3756..4616)
                     /locus_tag="ACH3L1_004016"
     CDS             complement(3756..4616)
                     /locus_tag="ACH3L1_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFT4078534.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACH3L1_004017"
     CDS             complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACH3L1_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFT4078535.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACH3L1_004018"
     CDS             complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACH3L1_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFT4078536.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1559 a         1543 c         1510 g         1479 t
ORIGIN      
        1 tatgagtcag tatgcatcgc cgcgatggaa catttaaaaa gtaaagcaac ggcccctata
       61 caaaacggac cgttgccagc ataagaatta acggtgtgcc agttcggcgt cgtcttcgct
      121 atccagaatg gctttatcgg tttgcttcag ccactggctg gtcagcgtgc cagctgtcat
      181 cgagccacta acgtttagcg ccgtacggcc catgtcgata agcggttcaa cggagattaa
      241 cagcgccacc agggttactg gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt
      301 tgcaccacca ccgacaccgg caacgcctgc ggaactaacg gtaacaatac cgaccagcgt
      361 cgcaatccac atcgggtcca gcgggttaat gccaaccgta ggcgcaacca tcaccgcagc
      421 attgccggat acaaaccggc gcagccgttc tgaccaatgg ttgcaccgaa agaggcggcg
      481 aaactggcga tggattcagg aacgcccaga cgacgcgttt gtgcttccac attcagtggg
      541 atagacgcag cactggaacg gctggtaaag gcaaacgtca gcacaggcca taccttacgg
      601 aagtacttca gcggactcac gccattaatg cccagcagaa tgccatgcac agcaaacata
      661 atcagcagac cgaggtagga cgcgacaacg aaacttccca gtttgatgat gtcttgcaag
      721 ttagaacctg caaccacttt ggtcattaga gccagaacgc cgtaaggggt caactgcatg
      781 acaaggcgaa ccagtttcat cacccagctt tgtagggtat cgatagcggt taagacgcgt
      841 tcccctttcg gcgcatcatc cttcagcagt ttcaatgcag ctacgccgag gaatgcggca
      901 aaaattacca cgctgataat tgacgtcgga ttggctccgg taagatcggc aaacgggttt
      961 ttcgggataa aggacaagac cagctgcgga acgctctgat cagagacttt accaacatag
     1021 ttactttcaa tggcgttcag acgtgcagtt tctgcaccac cctgaaccag accttcagcc
     1081 gtcaaaccaa acaggttggt gaccagcaca ccgaccagcg ccgcaatcag cgtggtaaac
     1141 aaaagcgtac cgatggtcag aaaactgatt ttgcctaact gagatgcgtt atgcagacgg
     1201 gcaaccgcgc tcagaataga ggcgaagact aacggcataa cgatcatttg cagcagttga
     1261 acatagccgt taccaacgat gttaaaccac tgtacagaat ctttaagtac ctggctgtca
     1321 gaaccataaa tggtatgcag ggcaaggcca aaaaccacac ccatcaccag acccaccagc
     1381 acttttttcg ccagactcca ctgtttatgg cgggtctgag ccagcgcaaa cagcagtaca
     1441 acgaacacca cgatgttcgc aattaatgga aagttcatcc ccgttctcct gatcttatta
     1501 tttctatcac tgcgtagcag tcatagtgtt ggcgcaagat taacagaact gtgagctggc
     1561 tcttatattc aaatggaatt gcttattcca gaaaggtgat ttttgccttt ttagtattca
     1621 acctgatgct taataagccg atgaaactgg atctgcaagg tattgatcat ttgcgatgac
     1681 caacgaacag cgctgttctc gggtaatgaa tggggcatcc ataccgacca gacaaataat
     1741 gccatgcaga taaaacgttc cagttggttg cccttttggg gaaccaggat aggcaagcgg
     1801 aaacgccagc ggcatggcca gagcagggga acaccggcgg gtgtcagcat atcggcaagt
     1861 atgtggctca aataacccag caccattcct tgtagcgcat cagccggaat gaaccagcct
     1921 tccggaacct taaggtaaaa ggttgccagc agcgcaaata ccgccagcag actgtgggta
     1981 aaaccacgat gcccaaaagc gcgggcgatc ggttttgata tccattttaa tcgctgccca
     2041 agaaacgact ttgggtgatc gatgtccggt aacaaacacg tcaggattgc ggaagggaca
     2101 atatgccacc agtcaccctg tgccagcacg ggcgtcagct cggcattttt ggcaaatacc
     2161 gcacaagcaa tagaaaagag aaggtgacct tccgccgtca tgataaaact cactaaactg
     2221 tcgatacgta cagtatagga tttttataca gtgcacggaa gtggtgacgg tgtaactctt
     2281 tctcttaaat tgacgattaa tccgtcagtt gaatgcaaat tcagataaag aaccaggggc
     2341 agcgtgatca ctgcccctgg tcattaaccg agaaggtctt ttgcggtgag ttctgtcagc
     2401 gatgagagtt tgacgtttgc taatacaaaa cgtggatcat tttgcgcttc tggcgcagga
     2461 acgacgatgg aacgcatgcg ggctgcttta gaagcgatca tgccatttac cgagtcttcc
     2521 agcgccacgc aggtcagtgg gtcaacgccc agttttgctg cgcagtcgag atatacttgc
     2581 ggatgcggct tgctgtaagg cagtttttca gccgaggcga gggcatcgaa actgtcgcgt
     2641 aagtcaaaca tggtcaacac tttttccagc atatgtagtg gtgacgcgga ggccagtccc
     2701 accaataaac cttgttcttt gcataacgca acagcttcgc gcacgcctgg taataatgga
     2761 cgtgtctctt caaccagtga aatggcacgg gcaataaccc gttctactac ttcctgacgg
     2821 cttggcccat tccacggttg ccgggcgtac caaagatcga ccaccatatc gatgcgtaaa
     2881 cctaaggtgt ccggcagctc gttacgacgg gagatatcta cccccaggct tgccatcaca
     2941 tccagttcgg ctcgatccca taaaggttct gagtcgataa gtaatccatc catatcaaaa
     3001 attgcagcaa gaatctgacg cggggttgac attacaacct ctcctttaga gcattatgtg
     3061 taaggtatta agccgtgctt atgattaagc gcctatcata tcgcctttaa agaatgggcg
     3121 aaaacagata tctacaggta aactaagtga aacgacgcgt cttaatcaag gagaactcat
     3181 gacgtatcaa caagctggac gcattgctgt tttgaaaagg attttgggtt gggtgatttt
     3241 tatacctgca ttgatctcta cattgatttc tttgctgaaa tttatgaata cccggcagga
     3301 aaaccaggaa ggcattaatg cggtcatgct cgactttgct catgtcatga tcgatatgat
     3361 gcaggcgaat acgccttttt tgaatctgtt ctggtataac tccccgaccc ctaattttaa
     3421 tggtggtgtg aacgttatgt tctgggtgat tttcatcctg atttttgtcg gactggcgtt
     3481 gcaggattcc ggtgcccgga tgagtcgcca ggcgcgcttc ttgcgggaag gtgttgaaga
     3541 tcaactcatt ctggaaaaag ccaaagggga agaggggcta acgcgtgaac aaattgagtc
     3601 ccgtattgtt gttccgcacc atactatttt cctgcagttt ttctcactat atatcctgcc
     3661 ggttatttgt attgctgcag gttatgtgtt cttttctctg cttgggttta tttaatcaaa
     3721 aggccgcacg gagcggccat tatcctcaac ccatatcatg ctgctaataa tctatccaat
     3781 gactgctgag caataaccaa atgctgaccg ccaaataatc ttgcacgatt taacagcgtg
     3841 tagagttggt aaaccggttg acgttcaagg aaatcggcag gtagcgggga tactgactga
     3901 tagccgtcat agatttgtgg cggctgttca gtatgcagcg gtaacatcgc taggtcgcac
     3961 tctcggtcac cccagtcgca ggcagggtcg aaaatgtacg ggccatccgg acccagcgca
     4021 cagttgccgg accataaatc gccgtgcaac agagaaggct gcggttgatg cgaggccaga
     4081 cgctgctgaa tatgctcgac gatggcgtcg atattgccga aagcgatccc tttctctgct
     4141 gccagttcca actgccagcc aatccgttgt tcagcaaaaa acgttgacca gcgacgttgc
     4201 caggtgttgg gctgtggagt tgtggagagc gagttatcaa aatcgaggcc aaattgtggt
     4261 tggtcactcc attgatgtaa acgcgcaatt tgctgaccaa gaataaatgc gctatgcgca
     4321 tccagcggac gaggtgggag ataatccatc accagaaaac tgtagtcacg gtcagcgcct
     4381 actgcccaaa ccttaggcac ggtgacggtt ttactacgcg acagtaactc cagttggtcg
     4441 gcttcggcgg taaaaccggg aagcagttcc ctttcatcac atttgacgaa aaagtcatgt
     4501 cctgcatagc gcaaatgcca tgcggcatgg acttctccgc caggcagttc attacgcagt
     4561 tcgatttcgc cttcacctaa ctgctcgctc aaaagacgac tgattgcctg ccacatgatg
     4621 tctctcccat gttgtctgcc agatcataag gttagcgcaa gaatgcggtg taaaaacacg
     4681 agctaacgca caccacagac tgattattgc ccaaaaacca tttattttct gttgctattc
     4741 cattcctccc aggtcgtgat ggtgatttca ggatctttgc cggttgcact ttgggtaagc
     4801 cccgaaacca gttctctaat ttcttcttca ctttgggtac tgataagtcc aaaagtgtta
     4861 gtccccagct catggatatt tccctcatca tccgtcatgg tgagcaaaaa accgtcgcga
     4921 gtcaggtaat tattcaactc gttaatttca gtcaatgtat cctcatgcaa catgacggtt
     4981 atgacataat ggacaagatc gccactggcc atagttcacc cctttgttat cacatgactt
     5041 ttgagcatag tcggagaaac gcgttgccga caggttggtg gtgattcccc caatgctggg
     5101 ggaatgtttt tgttagcggg aaaggtaagc gtaaattttt tgcgtatcgt catgggagca
     5161 cagacgtgtt ccctgattga gtgtggctgc actccccgca gctacgccaa aacggaccat
     5221 ctcttcaaga gaggcatttt ctgccagttt cagtgtcatc gcgccgacca tgctgtcacc
     5281 agcgccaacg gtactctggc ttttcaccgg tggtggcacc acctgaatgc agttttcact
     5341 atcgacaccc agcgctcctt gtggaccaag ggaaacgaca acccgtttgg ccttgccgct
     5401 attaacgatt tcctgcgcgg ctttgcggac atcgtctggc tgggtgagtt cgcgattcac
     5461 cagcgcactg agttcttttt ggttaggctt aaccaactcg atgttaccaa tcgccagtgc
     5521 tgcacttaac gcttcgccag aactgtcgat gatgcagcgg atcccttggt tttgcgcagc
     5581 ggaaatcagt tgggttaatt tttccagctt cacacctggc ggcaggcttc cgcttatgac
     5641 caggatggcc ccggattcaa tttccagaac ttgctcttca agctggcgaa actcatcttc
     5701 atttaatgcc gcgcctggca taacaaaacg atactgttca ccgcttgctt ccacatgtac
     5761 gtgtaaattc tgccgggtcc agtctttggc ttctacagta gcgacgggga cattttcatc
     5821 cgccaacagt gaaaccaggt gttcgccggt cgcgccaccc gccgggaaga tcgctgtggc
     5881 actgcctcca agatgggcaa tggcgcgggc gacgttgatg ccgccgcccc cgggttcgaa
     5941 caccggtgcg gtacagcgca gttttccttc gggataaatt tgcggggtaa ttgttgcgct
     6001 atcgagagag ggcgcaagtg tcaacgtata gatacgtacc atcatttcct cctataggct
     6061 gatttcagtc tggcaccgaa tcagcggaaa g
//
LOCUS       DAZXUL010000187         6058 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZXUL010000187 DAZXUL010000000
VERSION     DAZXUL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.188"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..143)
                     /locus_tag="ACH3L1_004019"
     CDS             complement(<1..143)
                     /locus_tag="ACH3L1_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HFT4078537.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="ACH3L1_004020"
     CDS             379..651
                     /locus_tag="ACH3L1_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFT4078538.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACH3L1_004021"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACH3L1_004021"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFT4078539.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="ACH3L1_004022"
     CDS             1111..1731
                     /locus_tag="ACH3L1_004022"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFT4078540.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="ACH3L1_004023"
                     /pseudo
     CDS             complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="ACH3L1_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3114..3767)
                     /locus_tag="ACH3L1_004024"
     CDS             complement(3114..3767)
                     /locus_tag="ACH3L1_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFT4078541.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="ACH3L1_004025"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="ACH3L1_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6058)
                     /locus_tag="ACH3L1_004026"
                     /pseudo
     CDS             complement(5211..6058)
                     /locus_tag="ACH3L1_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1621 c         1599 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DAZXUL010000188         6052 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZXUL010000188 DAZXUL010000000
VERSION     DAZXUL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.189"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            430..1338
                     /locus_tag="ACH3L1_004027"
     CDS             430..1338
                     /locus_tag="ACH3L1_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFT4078542.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="ACH3L1_004028"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="ACH3L1_004028"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFT4078543.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="ACH3L1_004029"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="ACH3L1_004029"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFT4078544.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="ACH3L1_004030"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="ACH3L1_004030"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFT4078545.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="ACH3L1_004031"
     CDS             complement(5673..>6052)
                     /locus_tag="ACH3L1_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078546.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1576 c         1561 g         1424 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 tcgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DAZXUL010000189         6007 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZXUL010000189 DAZXUL010000000
VERSION     DAZXUL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.190"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..281)
                     /gene="yneK"
                     /locus_tag="ACH3L1_004032"
     CDS             complement(<1..281)
                     /gene="yneK"
                     /locus_tag="ACH3L1_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFT4078547.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPV"
     gene            355..1296
                     /locus_tag="ACH3L1_004033"
                     /pseudo
     CDS             355..1296
                     /locus_tag="ACH3L1_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            1360..2286
                     /gene="glsB"
                     /locus_tag="ACH3L1_004034"
     CDS             1360..2286
                     /gene="glsB"
                     /locus_tag="ACH3L1_004034"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFT4078548.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            2286..2630
                     /locus_tag="ACH3L1_004035"
     CDS             2286..2630
                     /locus_tag="ACH3L1_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFT4078549.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            2784..4202
                     /locus_tag="ACH3L1_004036"
     CDS             2784..4202
                     /locus_tag="ACH3L1_004036"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFT4078550.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            4429..5880
                     /gene="uxaB"
                     /locus_tag="ACH3L1_004037"
     CDS             4429..5880
                     /gene="uxaB"
                     /locus_tag="ACH3L1_004037"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFT4078551.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1415 a         1362 c         1611 g         1619 t
ORIGIN      
        1 ttgacaggat aagtgagttt aaactgaacc ttttcattgt atagtgtgtc gttaccccga
       61 aaagaagtcc tctgaatata tgataagtta tcaaggttgt taaatatctt ttttgtggct
      121 gagttggtgt ttaaagacgt gcgccactct tcatcacata ttatggtact gagtgcatca
      181 ataaaatcct ttaaaacttc ttttacttga ggtgcaatat tacgagcggg aaaatcatca
      241 ggaagagata tgtaattgca gatgcttact ggcgtaacca tatttgcccc atatagtcat
      301 aagtcatcgg aacatcacta tatagggcat ttgagctgca agaactatca aaggcaggtg
      361 atgcgtggcg ctgttcccat cattcttgca ggtaacggct acttacttaa acatgcgccg
      421 aatgtgatgg gctgtgctca gctcattgcc caagtgttta aagatgcggg aatcccgcaa
      481 ggcgtatatg gctggctgaa tgccgacaac gacggtgtca gtcagatgat taaagactcg
      541 cgcattgctg ctgtcacggt gaccggaagt gttcgtgctg gagcggcact gaaaaaatgc
      601 gtactggaac tgggcggttc ggatccattt attgtgctta acgatgccga tctggaactg
      661 gcggttaaag cggcggtagc cggacgttat cagaataccg gacaggtttg tgcagcggca
      721 aaacgcttta ttatcgaaga gggaattgct tcggcattta ccgaacgttt tgtgtcagct
      781 gcggcagcct tgaaaatggg cgatccccgt gatgaagaga acgctctcgg accaatggct
      841 cgttttgatt tacgtgatga gctgcatcat caggtggaga aaaccctggc gcagggtgcg
      901 cgtttgttac tgggcgggga aaagatggct ggagcaggta actactatcc gccaacggtt
      961 ctggcgaatg ttaccccaga aatgaccgcg tttcgggaag aaatgtttgg ccccgttgct
     1021 gcaatcacca ttgcgaaaga tgcagaacat gcgctggaac tggctaatga tagtgagttc
     1081 ggcctttcag cgaccatttt taccaccgac gaaacacagg ccagacagat ggcggcacgt
     1141 ctggaatgcg gtggggtgtt tatcaatggt tattgtgcca gcgacgcgcg agtggccttt
     1201 ggtggcgtga aaaagagtgg ctttggtcgt gagctttccc atttcggctt acacgaattc
     1261 tgtaatatcc agacagtgtg gaaagaccgg atctgacgcg acatattcag ctctgatata
     1321 ctcgcaggtc ttttcagacc tgcggtccag gagtagaaag tggcagtcgc catggataat
     1381 gcaattttag aaaacatctt gcggcaagtg cggccgctca ttggtcaggg taaagtcgcg
     1441 gattatattc cggcgctggc tacagtagac ggttcccgat tggggattgc tatctgtacc
     1501 gttgacggac agctttttca ggccggagac gcgcaagaac gtttttccat tcagtctatt
     1561 tccaaagtgc tgagtctcgt tgtcgccatg cgtcattact ccgaagagga aatctggcaa
     1621 cgcgtcggca aagatccgtc tggatcaccg ttcaattctt tagtgcaact ggaaatggaa
     1681 cagggtattc cgcgtaatcc gttcattaat accggtgcgc tggtggtctg cgatatgttg
     1741 caagggcgac taagcgcacc acggcaacgt atgctggaag tcgtgcgcgg cttaagcggt
     1801 gtgtctgata tttcatacga tacggtggta gcgcgttccg aatttgaaca ttccgcgcga
     1861 aatgcggcta tcgcctggct gatgaagtcg tttggcaatt tccatcatga cgtgacaacc
     1921 gttctgcaaa actactttca ttactgcgct ctgaaaatga gctgtgtaga gctggcccgg
     1981 acgtttgtct ttctggctaa tcaggggaaa gctattcata ttgatgaacc tgtggtgacg
     2041 ccaatgcagg cgcggcaaat taacgcgctg atggcgacca gtggtatgta ccagaacgcg
     2101 ggggagtttg cctggcgggt gggactaccg gcgaaatctg gcgttggtgg cggtattgtg
     2161 gcgattgttc cgcatgaaat ggccatcgca gtctggagtc cggaactgga tgatgcaggt
     2221 aactcgcttg cgggtatcgc cgtccttgaa caattgacga aacagttagg gcgttcggtt
     2281 tattaatgca gtctctcgat ccactcttcg cgcgtttatc ccgttcaaaa tttcgctctc
     2341 gctttcgtct gggcatgaaa gagcgtcagt attgcctgga gaaaggcgcg ccagtcatcg
     2401 aacaacatgc ggcggatttt gtcgctaaac gtcttgctcc ggcattaccg gctaatgacg
     2461 gtaagcaaac ccccatgcgc gggcatccgg tgtttatcgc tcagcacgcc accgcgacct
     2521 gctgtcgcgg ttgtctggct aagtggcata acattccgca gggtgtttcg ttaagcgaag
     2581 aacaacaacg ttatatcgtg gcggtaatct atcactggct ggttgtatag atgaatcagc
     2641 catgagaatt gcggaaaacg ataaaatagc attcccaggg tacctttatt tgccgccgct
     2701 gttagcgaac atgctatcct ttatgagaat ttacttatca cgttgagata attgccaccc
     2761 gcaattgtat ggacagataa ttaatgcatg tacaccccat ttcaactttc cgtttgttcc
     2821 aggaaggtca tctgctacgt aatagcatcg ctatttttgc gctaaccacg ctgttctatt
     2881 ttattggtgc agagttacgg ctggttcacg aactttctct tttctggccg ctgaatggcg
     2941 taatggcggg ggtgtttgcc cgctatgtct ggcttaatcg actgcattac tatgcgatca
     3001 gttatgtggc gatgctggtt tatgatgcca taaccaccga atggggactg gtttcactgg
     3061 ttatcaattt ctccaatatg atgtttattg ttaccgtcgc cttactggtc gtgcgggata
     3121 agcgtctggg gaaaaataag tatgagccag taagtgcttt acggctattt aattactgtc
     3181 tgattgccgc attattatgc gctattgtcg gggcgattgg ttcggtcagt attgatagtc
     3241 tggatttctg gcctttgctt gcggactggt tcagtgagca attctcaacg ggcgtgttga
     3301 tcgtgccttg tatgctgacg ttggctattc ctggagtgct gccgcgcttt aaagcagaac
     3361 agatgatgcc cgccatcgcg tttattgtgt cggttattgc ctcggtagtc attggcggag
     3421 cggggagtct ggcgttcccg ctgcctgcat taatctggtg tgcagtgcgc tatacgccgc
     3481 aggtaacatg tctgttgacc tttgtcaccg gtgcggtgga aatcgtactg gtggcaaatt
     3541 cggtgattga tatctcggtc ggttcgccgt tctccattcc ggaaatgttc tccgcacgtc
     3601 tcggtattgc cacgatggcg atatgcccaa ttatggtttc ttttagcgtg gcagcgatca
     3661 atttgctaat gaagcaagtt gcgctgcgag ccgactttga ttttctgact caggtttact
     3721 cacggtccgg tctttatgag gcgctgaaaa gtccatcgct gaaacagaca caacatctga
     3781 cggtcatgct gcttgatatc gactatttca aaagcattaa cgataactat ggacatgaat
     3841 gtggcgataa agtgttaagc gtgtttgccc ggcatattca gaagattgtc ggtgataagg
     3901 ggctggtggc gcgcatgggc ggcgaagaat ttgctgttgc agtgccgtcg gtgaatcctg
     3961 tcgatggtct gctaatggcg gaaaaaatcc gtaaaggcgt tgaactgcaa ccattcacct
     4021 ggcaacaaaa aacgctttat ctcacggtaa gtattggcgt cggtagtggt cgcgcatcgt
     4081 accgaacgct gaccgatgat tttaataaat tgatggtcga agccgatacg tgtctgtatc
     4141 gctcgaagaa agatgggcgc aaccgtacca gcactatgcg ttacggtgag gaagttgtct
     4201 gacatcttta ttcattccgc gcgatttgaa tgcaatgtgt tctaccactt tatgatccag
     4261 gaaaaggcgt atttcctcga ttaaaccatg atccgcgcca cacttttctc ataaaactgc
     4321 tcattaatca tcaggagttg atcaaaacag gggttcggcg tattaaagtt tgctaacctg
     4381 tcgcaccaat tcaaacgggt tgtatgacta atcagaaggg aacccattgt gaaaacacta
     4441 aatcgtcgcg attttcccgg tgcacagtat ccagaacgta tcattcagtt tggtgaaggt
     4501 aacttcctgc gcgcctttgt tgactggcaa atcgatctcc tgaatgagca caccgatctg
     4561 aattctggcg tggtcgttgt tcgtccgatt gaaacttcat tcccgccgtc actgagcacg
     4621 caggatggtc tgtacaccac cattatccgt ggcctgaatg agaaggggga agcggtcagc
     4681 gacgcgcgtc tgattcgctc ggttaatcgt gaaatcagcg tctacagtga atacgatgaa
     4741 ttcctgaaac tggcgcacaa cccggaaatg cgctttgttt tctctaacac caccgaagcg
     4801 ggtatcagct accatgcggg cgataaattc gatgacgcgc cagcggtaag ctatccggca
     4861 aaactgaccc gtctgctgtt cgaacgcttt agccatttca acggtgcgct ggataaaggt
     4921 tggatcatca ttccgtgtga gttgattgac tacaatggcg acgcgttgcg tgaactggtg
     4981 ctgcgctatg cgcaagagtg ggcactgcca gaagcattta ttcaatggct ggatcaggct
     5041 aacagcttct gttctacgct ggtggaccgt atcgttaccg gttatccgcg cgatgaagtg
     5101 gctaaactgg aagaagaact gggttatcac gatggtttcc tcgacaccgc tgaacacttt
     5161 tacctgtttg tgattcaggg gccgaaatcc ttagcgactg agctgcgcct ggacaaatat
     5221 ccgctcaacg tgctgattgt tgacgatatt aaaccgtata aagaacgcaa agtggcaatc
     5281 ctcaacggtg cgcacaccgc gctggtgccg gtggcgttcc aggcagggct ggataccgta
     5341 ggtgaagcga tgaacgatgc tgaaatttgc gcattcgttg aaaaagccat ctacgaagaa
     5401 attattccgg tactggattt gcctcgtgat gaactggaat ctttcgccag tgcagtcacc
     5461 gggcgtttcc gtaacccgta cattaagcat cagctgctgt ctatcgcgct caacggtatg
     5521 accaagttcc gcacccgcat cctgccgcag ctgctggcag ggcagaaggc aaatggcaca
     5581 cttccggcgc gccttacttt cgcattagcg gcattgattg cgttctatcg tggtgagcgt
     5641 aacggagaaa catatccggt acaggacgat gcacactggc tggaacgtta tcagcaactc
     5701 tggagccagc atcgtgaccg tgttatcggt acccaggaac tggtagcgat tgtgttggca
     5761 gaaaaagacc actgggagca ggacctgacg caagtgcctg gtctggtcga gcaggttgct
     5821 aatgacctgg atgcgattct tgaaaaaggg atgcgcgaag ctgtacgtcc gttgtgctaa
     5881 taacaccgtt acttctgaac ccggtttcga ccgggtttct tatttcacta tgttagttac
     5941 aacatcctat attcctttat cattaacagg tgtcacatgc agcgcctgtt aatgttgtaa
     6001 gcttgac
//
LOCUS       DAZXUL010000190         5984 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZXUL010000190 DAZXUL010000000
VERSION     DAZXUL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.191"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(118..436)
                     /locus_tag="ACH3L1_004038"
                     /pseudo
     CDS             complement(118..436)
                     /locus_tag="ACH3L1_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(580..3888)
                     /gene="icsA"
                     /locus_tag="ACH3L1_004039"
                     /gene_synonym="virG"
     CDS             complement(580..3888)
                     /gene="icsA"
                     /locus_tag="ACH3L1_004039"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFT4078552.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4417..5619
                     /gene="virA"
                     /locus_tag="ACH3L1_004040"
     CDS             4417..5619
                     /gene="virA"
                     /locus_tag="ACH3L1_004040"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFT4078553.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5798..>5984
                     /locus_tag="ACH3L1_004041"
     CDS             5798..>5984
                     /locus_tag="ACH3L1_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4078554.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1771 t
ORIGIN      
        1 ttcaggctgt acgcttcccc atgtgatttg cctcgccact tcaccgggct caggaccccg
       61 taggatcttc accgaaaaaa atgcatcacc agttgcttta ttatttgttt taggtagacc
      121 tgttactaat ttatacctac tctgaccaag tctggataag aaaggcacac ctgcacattt
      181 ctacttggaa atgttttctt ttaccatttt gctcaaaaat ttcaacatcc aatattcctc
      241 gtacagaact actcaagtcc tgaatagaaa aagttccagc tggtacagta gagtcatata
      301 ttactcgacc ttgttgagat acaacaacac gtgcattagt ttctgcaata ccagtaatct
      361 gaggcgcata accacgaata tttggcggta gcattcgttc atcgctttct aatgcaattc
      421 ctgtgtaacg ccaagcaaca gcgcattctg gcgcatgatc tggtaaacac gtttggcatt
      481 gatcgcaggc ataccatcat gtgcacaaaa acccaccgag cttttacacg gtgggtccca
      541 gagaaatgca ggacatcaac acgccctgca tttttattat cagaaggtat atttcacacc
      601 caaaatacct tgggtgtctc tgtaactgtt attacccaat tgctgagata cgtttcccca
      661 gatgcttagg ttatctgcaa gacgcccccc cacaccagtt ttgagttcaa tcacattacg
      721 gttgcctatc tggtgatttg acatgccatt cataattaca ccatattgct ttgagctcca
      781 tttccaattc acttccaaaa atggttcgaa cttacgaaga gtctccttat cgatacaact
      841 ctgactaatt aaggatgtac gcaatcccaa ttttgcttga atattattgc ccccagagga
      901 tgtgaccata gtgccattgt gttcaatata gtcgtttgct tttaccccat gcctagttat
      961 ctggacttgt ggattgagat acaatgcgtt gtcaatatta ttatgagcag tccagcgtat
     1021 ggttgggtag atatagccag cttccaaagc tcctgttatt cctttgctgg aatatttctc
     1081 accagttaac ccatctcctt tgactgtatt attaaaccaa ctgtactgca accaagcatc
     1141 catatataga cctgttcgtt ctttttcatc ctgaaaccaa gaactgtaca aaccggcagt
     1201 ataaccgctc acagttcctc gtgaactata actagtatac ttattatgag ttttgctatc
     1261 ctgatatcca taagctccca taatacccac atgcaagcca tcatgatcag tgaagtttgt
     1321 cttcaaaata tctccaccaa tctgataaac catcctgtta gctgtggttt ttaattgtct
     1381 atcagctaaa ttatgctcat tatgtccacc aacagtacga atccacatgg tggtttcgct
     1441 gagctgacct gtagtaggat caacaatagt gctaccgtgt cggtcataga gagaaggact
     1501 aaacatcgtg ttcattgcta tcaggttagc aatataactg ccagcttcag ggcgccatgt
     1561 gtgaatacta ccttcaccca aattaagtga agatacaagc tgtgaactag ctcggcgact
     1621 actcatttga gtagactctt gattgctcat ctgttttgat tcttgattat ccatctgact
     1681 agttagatac cacttattgg tattcagtcc agatacagtg ccctgtttca ggcgatagtc
     1741 ataactacca gcaacaatac ggcctttctg aataaaagca ttcttatcag aagaatctgt
     1801 cgaaataatt tgaacacctt caagagtttt atctccagag cctccctcat ttgtcactgc
     1861 aacataggta ataccagacg catggcctgc aatttccagc cggtcagttt tactgttgtc
     1921 attgccgaga accgcgccca aagataatat tccgcccttt ccatgccaat tacctttctg
     1981 aacataggtc tgaccgacat ttgacgaact attattgaga ataacatgac cgtttttctc
     2041 cagagtcatg ttacctgtga caatgacggg gtcaggcaaa aaaggggcat taatattatt
     2101 gattagcaca gtcccactat ttaataaagt gttaacagtt tggttcatgc ctgaaaaatt
     2161 caaggtagca cctttattaa caataacacc agcggtacgt gtcatagctt caactgtccc
     2221 catttttaga atgcctgcat tgatgttggt gaaaccagag taggtatttt tttccgccaa
     2281 aatgagagtt ccctcccctg attttgttag ggattttccg tcccagctat ttatattatg
     2341 atggctgaga ttatctgcca gaatagtact aacagtaagt tcagcgtttt ctttcagatt
     2401 gaactctcca tgactgtcgc catctgtatc attccagctc aaaccgtaga tcaatttatt
     2461 ctggtcactg atatagcttt ttgtatagtc aatagcacca gtagtgagta acggattgat
     2521 gatagatata ttagaaacac tgaaacctgt tatcccttta tctgcactta gcagtttata
     2581 gtccttctgc gcccagttat ttataatagc ctccactttt acattgttgc caaatgacat
     2641 atttccagaa gcttccagac tactgattcc agctaaattt aaagaactgt tctcaccaaa
     2701 agaaacgtca cctgaaacag tgaatttgtt acctgatagt gaaacagttg tacaatcacc
     2761 agcacataaa tcaccttcaa tagttggtgt ggtcccagtg taatcattgt tgataatttt
     2821 cagaatggat gaattgttaa cctgtacatc accagtaatg acagaacctt catggagtat
     2881 taatgaattg tttccaccag tgatgtggat agcattacct gctacatttt gtgttccatc
     2941 atcttcttta cctgaaatta tatatccgct attagcaatg gtcatattgc taccatttat
     3001 agcatccccc caatgtccac cattaccgcc taaaatagct ccattgttga ttacagacag
     3061 gttatctcct gtgatagcat taccaccgta gccatcataa ccataaccgt aagcagcacc
     3121 tccgttgcct ccagcgaacg tgcccttatt gattacagag aggctgcttc ctgtgatggc
     3181 atcacctcca ttaccgccat caccctcacc ataattgtta cctccattac caccggaaat
     3241 agtccctttg ttgattatgg gtaaattgac tcctgtgacg gcatcacctc cattcccccc
     3301 atcactatca ctataactat cgccaccatg tccacctgaa ataatttctc catttattat
     3361 aaacagattg tctcctgtaa cagcctcacc accatcaccg ttatggtcag caccgctacc
     3421 gccgctacca ccaagaatca tgccttgatt gattatagac aagtcagatc cggtaataga
     3481 gtcaccacca ttaccgccac aactattacc atcattatta tcaccagcac caccatgacc
     3541 tgcgctaatc ataatatcag agttaatccg tagcttagtt ctagatgcat gagaggggtt
     3601 ggaagaagtt atataccacg catccattcc atcttcacca gctcccaagg gagaaagtcc
     3661 atcaacaggt gttaataatt tttcataatt gtcctctgaa aaatgaagtt cttgagtacc
     3721 cgaaagagga gtagcaaaag ctattggccc cccgagcaac agggatgcac caacaacaaa
     3781 aggagaaaaa gacaatttgc atgttttttc ctttaccgta ggtaattctc cggccccccc
     3841 ctgtgaacat tgggtcatat tacaaaaaaa tttgtgaatt tgattcatgc actatattat
     3901 cagtaagtgg ttgataaacc cctgaaaaga ttccgtttta tccggaataa agggacgata
     3961 tatgcaaaaa catattaaac aaagcctcaa ccaaaacatg agagctgtgt tatatatcaa
     4021 tgcaacggga gaaatttacc tgtgtcttgc tttgaaaaaa agagggggaa agctatatgt
     4081 tcgataataa attaataatt ttactatgat agtctgcttc agaccgcaac ttttcagttc
     4141 tttctgtacg cttgcccaaa gtttaaccac tttgtttttc tatacaccca atggttctat
     4201 ctgccccttg atgttaatcc ccaaagccaa taacatgaat gtattcatat cacaactttc
     4261 ctaccagact ctccaatgca ataactgcat aattgttttt agtggaaatg tatcgttttc
     4321 ttaaagagaa gaataacatt ccattttatt atgttcccaa tttaattata ttaatatata
     4381 ttggcattaa taggaaaata catcaggaga aatcaaatgc agacatcaaa cataactaac
     4441 catgaaagaa atgactcatc atggatgtca actgtcaaat caactacaga agtaagttgg
     4501 aataaattga gtttttgtga cattttactg aaaataataa ctttcggcat atattctcca
     4561 cacgaaactc tagccgaaaa acatagcgaa aaaaaactaa tggatagttt ctcaccatca
     4621 ctatcacaag ataaaatgga cggagagttt gcccacgcaa acatagatgg catttcaata
     4681 cggctatgcc ttaacaaagg catatgttcg gttttttatc ttgatggaga taaaatacaa
     4741 tccactcaac tttcatcaaa agaatataat aacttgttaa gttcactccc ccccaaacaa
     4801 ttcaacttag gaaaagtaca tactattaca gctccggtat cagggaattt taaaactcac
     4861 aagcctgcac cagaagttat tgaaacagcc attaattgct gtacatcgat aataccaaac
     4921 gatgattact tccacgttaa agacactgat tttaatagtg tttggcatga tatttacaga
     4981 gatattcgag catctgacag caatagtaca aaaatatatt tcaacaatat tgaaatacca
     5041 ctaaaattaa ttgcagatct aattaatgag ttaggaatta atgaattcat tgatagcaaa
     5101 aaagagcttc aaatgctaag ttacaaccaa gtaaataaaa taatcaattc aaacttcccc
     5161 caacaagacc tatgttttca aacagaaaaa cttttgttta cttcactctt tcaagaccct
     5221 gcatttatat ctgcactaac atctgcattc tggcaatctc ttcacatcac gtcttcctct
     5281 gtggaacaca tatatgccca aattatgtcc gaaaacatag aaaacaggct taatttcatg
     5341 cctgaacaac gagttattaa caattgtgga catattataa aaatcaacgc agttgtccct
     5401 aaaaacgaca ctgcaatctc tgcctctggg gggagggctt acgaagttag ctcatcaata
     5461 ttaccatcgc acataacatg taatggtgtt ggtattaata aaatagaaac aagctatctc
     5521 gttcatgctg gaacacttcc aagcagtgaa gggcttcgca atgctatccc tccagaaagt
     5581 cgtcaagtat catttgccat catatctcct gatgtttaag ccaatattat attggctgcc
     5641 agactgtaaa aaaacgttct tcagaacagc tctataaact attaaaccaa agctaaaagg
     5701 atggggacca atagaatctc agcccatcct ttttatatca ttatgaaccg ccccggaaat
     5761 cctggagact aaactccctg agaaagaggt aaacaggatg actaaaaata cacgtttttc
     5821 ccctgaagtc cgtcaacggg cagttcgtat ggttctggaa agtcagggcg aatatgactc
     5881 acaatgggca gcgatttgtt ccattgcccc aaagattggc tgtatactgg aaactctgcg
     5941 tgtctgggta cgccagcatg agcgggatac cgggggcggt gagg
//
LOCUS       DAZXUL010000191         5890 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZXUL010000191 DAZXUL010000000
VERSION     DAZXUL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.192"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..244
                     /locus_tag="ACH3L1_004042"
     CDS             <1..244
                     /locus_tag="ACH3L1_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078555.1"
                     /translation="EMLSFLTSEEREALTNMLARKGVETVLRLLDKETQELLQLEGRK
                     RLAALMLVDMPDEQVREILEEIEASILSESVNAKAG"
     gene            493..1437
                     /gene="tauA"
                     /locus_tag="ACH3L1_004043"
                     /pseudo
     CDS             493..1437
                     /gene="tauA"
                     /locus_tag="ACH3L1_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="ACH3L1_004044"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="ACH3L1_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFT4078556.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="ACH3L1_004045"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="ACH3L1_004045"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFT4078557.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="ACH3L1_004046"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="ACH3L1_004046"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFT4078558.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTNV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACH3L1_004047"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACH3L1_004047"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFT4078559.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5493..5861
                     /locus_tag="ACH3L1_004048"
                     /pseudo
     CDS             5493..5861
                     /locus_tag="ACH3L1_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1400 a         1455 c         1597 g         1438 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccaat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttggttatt
     5161 attttttggc gtttggttgt gctgctataa attgaataat tcaaactttg ttgtatattg
     5221 ataagatatt tatctggttt gtatttttga tatttataac gcgtatgcat caaaaataac
     5281 attatctgca acatactgaa atatcgtcct tttatcaatt tgatgcgtgt ttttctccat
     5341 aaattatata tgtccacatt cggacttagg ggaaagaata attgaaccat tcgtctgtaa
     5401 cgcagcataa tcgttagcgc gaaacataat atgttttcta tgcattgata attgatggat
     5461 caacttatta cgtccctgag gagggatgac aaatgcactc ctggaaaaag aaacttgtag
     5521 tatcacaatt agcattggct tgcactctgg ctatcacctc tcaggctaat gcagcgacca
     5581 acgatatttc tggtcaaact tacaatactt tccatcacta caacgacgcc acctatgctg
     5641 atgacgttta ctatgatggt tatgtaggct ggaacaacta tgccgctgat agctattaca
     5701 acggcgatat ctacccggtc attaataacg ctaccgttaa cggcgtgatt tctacctact
     5761 atctggacga cggtatttct accaatacca acgccaatag tctgacaatc aaaaacagca
     5821 ctattcacgg tatgatttat tccgagtgca tgacaactga ttagactggc cccctgaatc
     5881 tccagacaac
//
LOCUS       DAZXUL010000192         5821 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZXUL010000192 DAZXUL010000000
VERSION     DAZXUL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.193"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..411
                     /locus_tag="ACH3L1_004049"
     CDS             <1..411
                     /locus_tag="ACH3L1_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4078560.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="ACH3L1_004050"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="ACH3L1_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACH3L1_004051"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACH3L1_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFT4078561.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
     gene            5722..>5821
                     /locus_tag="ACH3L1_004052"
     CDS             5722..>5821
                     /locus_tag="ACH3L1_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078562.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPM"
BASE COUNT         1431 a         1458 c         1387 g         1545 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc gagaaccacg gcagcctgac ggctgcaaat tgaaaactgc
     5641 ccctgttcgt cctggtgttg ccgcacgctc cgcagaatat gtatgctctg aggacctgtt
     5701 ttattaagga ctcatcctct tatgttgacc cgcaaaagta tagataccgt tctgctctct
     5761 gttggtgctg agaagctctc acaacgtgaa tgggactgga tgaagatgct caaacccatg
     5821 g
//
LOCUS       DAZXUL010000193         5800 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZXUL010000193 DAZXUL010000000
VERSION     DAZXUL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.194"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(91..627)
                     /gene="ssb1"
                     /locus_tag="ACH3L1_004053"
     CDS             complement(91..627)
                     /gene="ssb1"
                     /locus_tag="ACH3L1_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFT4078563.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            881..3703
                     /gene="uvrA"
                     /locus_tag="ACH3L1_004054"
     CDS             881..3703
                     /gene="uvrA"
                     /locus_tag="ACH3L1_004054"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFT4078564.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3738..4094)
                     /locus_tag="ACH3L1_004055"
     CDS             complement(3738..4094)
                     /locus_tag="ACH3L1_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFT4078565.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4098..4514)
                     /locus_tag="ACH3L1_004056"
     CDS             complement(4098..4514)
                     /locus_tag="ACH3L1_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFT4078566.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4625..5338)
                     /gene="aphA"
                     /locus_tag="ACH3L1_004057"
     CDS             complement(4625..5338)
                     /gene="aphA"
                     /locus_tag="ACH3L1_004057"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFT4078567.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1395 a         1501 c         1493 g         1411 t
ORIGIN      
        1 agcctccata acggaggctt cttttttatg gctggcagat gctttaatca tccaccttaa
       61 aacaatataa cctattgttt taatgacaaa tcagaacgga atgtcatcat caaagtccat
      121 cggcggctcg ttagacggcg ctgccggagc ggactgctgc gggcgagact gcgcgccgcc
      181 gctgaactga ttgccaccct gcggctgctg aggctgaccc caaccgccct gcggctgacc
      241 accaccgata ttgccacctg ccggagcgcc accaccctga cgaccaccca gcatctgcat
      301 ggtgccgcca acgttcacca cgacttctgt ggtgtagcga tcctgaccgg attgatcggt
      361 ccatttacgg gtacgcagct gaccttcgat ataaacctga gaacctttac gcagatattc
      421 gctcgccact tctgccagtt tgccgaacag cacaacgcgg tgccattcag tctgttcttt
      481 catctcgccg gtcgctttat cacgccagga ttcggaagta gccagcgtaa tgttggcaac
      541 tgcgccacca tttggcatgt agcgtacttc cgggtcctga cccagattac caacgagaat
      601 aaccttgttt acgcctctgc tggccatgtt cgtgtctcct gaaaaaaatc gttctgaata
      661 agtgtaaacg cgcgattgta ccattaccaa tagcgctttt actatgttgt gacctcggtt
      721 ccgggaaaca aacctggcca gacattgtta cacaacactc cggataatgc attccaatac
      781 tgtatattca ttcaggtcaa tttgtgtcat aattaaccgt ttgtgatcgc cggtagcacc
      841 atgccaccgg gcaaaaaagc gtttaatccg ggaaaggtga atggataaga tcgaagttcg
      901 gggcgcccgc acccataatc tcaaaaacat caacctcgtt atcccccgcg acaagctcat
      961 tgtcgtgacc gggctttcgg gttctggcaa atcctcgctc gctttcgaca ccttatatgc
     1021 cgaagggcag cgccgttacg ttgaatccct ttccgcctac gcgcggcagt ttctgtcact
     1081 gatggaaaag ccggacgtcg atcatattga ggggctttct cctgccatct caattgagca
     1141 gaaatcgacg tctcataacc cgcgttctac ggtggggaca atcaccgaaa tccacgacta
     1201 tttgcgtttg ttgttcgccc gcgtcggcga accgcgctgt ccggaccacg acgtcccgct
     1261 ggcggcgcaa accgtcagcc agatggtgga taacgtgctg tcgcagccgg aaggcaagcg
     1321 tctgatgctg ctcgcgccaa tcattaaaga gcgcaaaggc gaacacacca aaacgctgga
     1381 gaacctggca agccagggtt acatccgtgc tcgtattgat ggcgaagtct gcgatctttc
     1441 cgatccgccg aaactggaac tgcaaaagaa acataccatt gaagtggtgg ttgatcgctt
     1501 caaggtgcgt gacgatctta cccaacgtct tgcggagtcg tttgaaaccg cgctggaact
     1561 ttccggtggt acagcggtag tggcggatat ggacgacccg aaagcggaag agctgctgtt
     1621 ctccgccaac ttcgcctgcc caatttgcgg ctacagtatg cgtgaactgg agccgcgact
     1681 gttttcgttt aacaacccgg caggtgcctg cccgacctgt gacggccttg gcgtacagca
     1741 atatttcgat cctgaccgcg tgatccaaaa ccccgagctg tcactggctg gcggtgcgat
     1801 ccgtggctgg gatcgccgca acttctatta cttccagatg ctgaaatcgc tggcagatca
     1861 ctataagttc gacgtcgaag cgccgtgggg cagcctgagc gcgaacgtgc ataaagtggt
     1921 gttgtacggt tctggcaaag aaaacattga attcaaatac atgaacgatc gtggcgatac
     1981 ctccatccgt cgtcatccgt tcgaaggcgt gctgcacaat atggagcgcc gttataaaga
     2041 gacagaatcc agtgcggtac gtgaagaatt agccaagttt atcagcaatc gcccatgcgc
     2101 cagctgcgaa gggacgcgtc tgcgtcggga agcgcgccac gtttatgtcg agaatacgcc
     2161 gctgcctgct atctccgaca tgagcatcgg tcatgcgatg gaattcttca acaatctcaa
     2221 actcgctggt cagcgggcga agattgcgga aaaaattctt aaagagatcg gcgatcgcct
     2281 gaaattcctc gtaaacgtcg gcctgaatta cctgacactt tcccgctcgg cagaaacact
     2341 ttccggcggt gaagcccagc gtatccgtct ggcgagccag attggtgcgg gcctggttgg
     2401 cgttatgtac gtgctggacg agccgtctat cggcctgcac cagcgcgata acgagcgcct
     2461 gttgggtacg cttatccatc tgcgcgatct cggtaatacc gtgattgtgg tggagcatga
     2521 cgaagacgca attcgcgccg ctgaccatgt gatcgatatc ggtcctggtg cgggtgttca
     2581 cggcggtgaa gtggtcgcag aaggtccgct ggaagcgatt atggcggtgc ctgaatcgtt
     2641 gaccgggcag tacatgagcg gtaaacgcaa gattgaagtg ccgaagaaac gcgttccggc
     2701 gaatccagaa aaagtgctga agctgacagg cgcacgcggc aacaacctga aggacgtgac
     2761 gctcacgctg ccagtcggtc tgtttacctg catcacaggg gtttcaggtt ccggtaaatc
     2821 gacgctgatt aacgacacac tgttcccgat tgcccaacgc cagttgaatg gtgcgaccat
     2881 cgccgaaccg gcaccgtatc gcgatattca gggactggag catttcgata aagtgatcga
     2941 tatcgaccaa agcccaattg gtcgtactcc acgttctaac ccggcgacct ataccggcgt
     3001 gtttacgcct gtgcgcgaac tgtttgcggg cgtaccggaa tcccgtgcgc gtggttatac
     3061 gccaggacgt ttcagcttta acgtccgtgg cgggcgctgc gaagcctgtc agggcgacgg
     3121 tgtgatcaaa gtggagatgc acttcctgcc ggatatctac gtgccgtgcg atcagtgcaa
     3181 aggtaaacgc tataaccgtg aaacgctgga aattaagtac aaaggcaaaa ccatccacga
     3241 agtgctggat atgaccatcg aagaggcgcg tgagttcttt gatgcggtgc cagctctggc
     3301 gcgtaagctg caaacgttga tggacgttgg tctgacgtac attcgcctgg ggcagtccgc
     3361 aaccacactt tctggtggtg aagcccagcg cgtgaagctg gcgcgtgagc tgtcaaaacg
     3421 cggcaccggg cagacgctgt atattctcga cgagccgacc accggtctgc acttcgccga
     3481 tattcagcaa ctcctcgacg tgctgcataa actgcgcgat cagggcaata ccattgtggt
     3541 gattgagcac aatctcgacg tgatcaaaac cgctgactgg attgtcgacc tgggaccaga
     3601 aggcggcagt ggcggcggcg aaatcctcgt ctccggtacg ccagaaaccg tcgcggagtg
     3661 cgaagcttcg catacggcac gcttcctcaa gccgatgctg taatcgttaa ggccgctttc
     3721 tgagcggcct tttcctttca gagttgtacc agcaatttac gcttttcttc cggtagtaaa
     3781 ttcactgcct gctgataaga cgcatccacc agataataga tttgcgaatc cggcagcgaa
     3841 ccgtcgagat acacggtgct ccagtgcgct ttattcagat ggcggctagg acgcacatcg
     3901 ctgtgctgct gacgtagcag ctccgccagc tccgggctgg ttttcagcga aacagctggg
     3961 cgattttcta cttctttcac catcgcaaac agtacatctt ccactttgat ctgcgtcgct
     4021 ttccagtcgt tatgcacgct ctgttctgcg cttggttttg ccatgcaata ttgtagcaac
     4081 tccgaaatgg tcatttttta ctccccttgt agtgtcgcga tgatgcgacg cgatccgccg
     4141 tggatgcgat gttcccccag ccagatgcct tgccaagtgc cggtctgaat acgcccttta
     4201 tgtaccggca atacaagcga tgttcccagc attgaggatt tgatatgaga aggcatatcg
     4261 tctgctccct catagtcatg ctcataattt ccgttgtcgg gaacggtgcg gaggaaaaaa
     4321 cactccatgt cgtggcgtac ggtgggatcg cagttctcat taagtgtcag agaggcggag
     4381 gtatgttgca gcaacagatg cagtaagccg atgttaacgc gcggcatatc agccagctga
     4441 ttcagaattt catccgttac cagatgaaac ccacgagatt tggcgctaag cgtgagcgtc
     4501 ttttgatacc acatgtgctg ctccttgata aaactctctt aatcagtttg cagcaagaca
     4561 gcgaaaggat aaaggtgtga ttaaaaaaac agcattcggg agagcatcac tctctcccgc
     4621 tctgtcagta ttctgaattg acgatcacct cttcaccaaa cgcacccgct tgtggcaagg
     4681 gtttgtaggt agagttggag gcgcgcagaa tgcggatacc acgagcgccg acatcgcgtg
     4741 cggcggtaat atcgttatcg gaatcgccat aaaaaattcg gatattttta tcctgcagcc
     4801 attgcgattt tgtattttgc cctggtttat cgcccgcaaa gattaccgga ttcatgttgg
     4861 tggcaggaat atgaaaatta tccgccagcg tttttgaaac cgtttctgtt ttcgtcgggc
     4921 tacgaccagt cacaaagaag atcgcgtcac cgcggcgtac atgcatatca atcagctggc
     4981 gagcgacctc ttttggaatg ctgaattcat cccagccatt attcattttt tcccagaaca
     5041 caggattttt cagataatct tcgctttctg gcgagaaggt ttttttgccg cgccagaacc
     5101 ccggactgga aaaaagtacc gtgtcatcga tatcaaaccc caccgccatt ggcggacgcc
     5161 ctgcgaggct attttcaatt tgtgcgaccg aaacccaatg aattggtgcc tgttcagcaa
     5221 gcctggcaac gttagtccca gggttaagcg gtgaaggaga tgaggccagg gcaacagcgg
     5281 aactgtttag agcgaacaat aagcaaacgg cactgattgc ctgtgtgatc ttgcgcatat
     5341 ttttccctaa atagtcagta tgttgaaact ttttattgtg agatttgttg caaaaaccat
     5401 ctgaccataa cgccagctgc cggggaaagg aaggattatc tgcggttttt gtgatgaaaa
     5461 gcaggatgat tatatttaac ccagtaaata cgttcctgtc tttttttgta taaaaaatgt
     5521 ttattactcg atgtaaggat acattaattt tacgtttatt atgatgaatc ctcggcaaat
     5581 gagcgaaatt tgatagaaca tttatctgct gtacgattta atgaacttaa tgatacagca
     5641 ttacaaaaac aatcgaagtt tataaagatg atttctgatt gaccagcccc ctaccctaca
     5701 atggtgatta attttattac tcgcgatgga gatgatgata tgaatatatc atatgtgaat
     5761 agcaacaaaa cgacatcttt acctgttgag ctagatgcac
//
LOCUS       DAZXUL010000194         5800 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZXUL010000194 DAZXUL010000000
VERSION     DAZXUL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.195"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..946
                     /gene="lpfD-O113"
                     /locus_tag="ACH3L1_004058"
     CDS             <1..946
                     /gene="lpfD-O113"
                     /locus_tag="ACH3L1_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFT4078568.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
     gene            1194..2234
                     /gene="pstS"
                     /locus_tag="ACH3L1_004059"
     CDS             1194..2234
                     /gene="pstS"
                     /locus_tag="ACH3L1_004059"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFT4078569.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEAWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2321..3280
                     /gene="pstC"
                     /locus_tag="ACH3L1_004060"
     CDS             2321..3280
                     /gene="pstC"
                     /locus_tag="ACH3L1_004060"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFT4078570.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3280..4170
                     /gene="pstA"
                     /locus_tag="ACH3L1_004061"
     CDS             3280..4170
                     /gene="pstA"
                     /locus_tag="ACH3L1_004061"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFT4078571.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4262..5035
                     /gene="pstB"
                     /locus_tag="ACH3L1_004062"
     CDS             4262..5035
                     /gene="pstB"
                     /locus_tag="ACH3L1_004062"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFT4078572.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5050..5775
                     /gene="phoU"
                     /locus_tag="ACH3L1_004063"
     CDS             5050..5775
                     /gene="phoU"
                     /locus_tag="ACH3L1_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFT4078573.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1436 a         1398 c         1516 g         1450 t
ORIGIN      
        1 gggatatctt attaccgcga acgaaaataa ggttggaacg acatttaaca ccactgtaac
       61 taatggatcc tcatatcttg gacgctgtca ttgtgatact ggtaacgtag gagaattccc
      121 ctatatctat tatacgtcaa aaataaacca ggcattaact tatgctgggg ttcactctaa
      181 tattaattat tatgatctta atccgaacct tgatgttggg attgctatag atattcttgg
      241 ggttgggtat gtgaatgcac cttttgaata tcatgctaac aacccgtctg gtaatacaaa
      301 gtacaattgc aaccgcattg aacctttaag tatatccagt ggggctaaag cgatagtata
      361 tttttatatc aagaagacat ttgcaggaaa attgattatt cctgaaacaa agatagtgac
      421 attgtatgga acaattagcc gtgacacccc ggtggattac tcacagccga tggctgatgt
      481 ttatattcgg ggcgatatta cagctccgca aagttgcgaa ataaataatt taaagccagt
      541 ttgttttgat tttaaagaaa taccagctgc agatttttca tctgttgttg gaagtgcggt
      601 aacaacacat aaaattacta aaacagtcac tattgagtgt gaaaacctgg ggatactgaa
      661 taccgatgat atcagtacgt ctttttatgc taccgaaccc aatacggaca attcaatggt
      721 cgtaacttca aactctaacg tggggataaa aatttacgac aaaaataata aggaaatcaa
      781 agtgaacggt ggtgagttgc caacagacat ggataaatca acagtgtatg gtgaaaaatc
      841 aggtagtgta actttttcag ctgctcctgc gagccttact ggagctcgcc cagccccggg
      901 gcaatttact gcaacagcga cgataactgt tgagattgtg cgctaatagg tctgacaacg
      961 attgttcatc acatcgttgt catctcaccc atttctttat cacttttaaa tgcatcatga
     1021 caaaaagatg gcatgtggaa ttttcatctt cacatttaaa atcagtaagt tatttggtaa
     1081 ttcctttctg tcataaaaat gtcatattcc ttatatataa ctgtcacctg tttgtcctat
     1141 tttgcttctc gtagccaaca aacaatgctt tatgaatcct cccaggagac attatgaaag
     1201 ttatgcgtac caccgtcgca actgttgtcg ccgcgacctt atcgatgagt gctttctctg
     1261 tgtttgcaga agcaagcctg acaggtgcag gtgcaacctt ccctgcgccg gtgtatgcca
     1321 aatgggctga cacttaccag aaagaaaccg gtaataaagt taactaccag ggtatcggtt
     1381 cttccggtgg cgtaaaacag attatcgcta ataccgttga ttttggtgcc tctgacgcgc
     1441 cgctgtctga cgaaaaactg gctcaggaag gtctgttcca gttcccgacc gtgattggcg
     1501 gcgtggtgct ggcggttaac attccagggc tgaagtctgg cgaactggtg ctggatggta
     1561 aaaccctcgg cgacatctac ctgggcaaaa tcaagaagtg ggatgatgaa gccatcgcca
     1621 aactgaatcc gggtctgaaa ctgccttcac aaaacattgc tgtagtacgc cgcgcagatg
     1681 gctccgggac ttccttcgtc ttcaccagct acctggcgaa agtgaacgaa gcgtggaaaa
     1741 acaacgttgg tactggctct accgtaaaat ggccgatcgg tctgggcggt aaaggtaacg
     1801 acggtatcgc cgcgttcgtt cagcgtctgc cgggtgcaat tggttatgtt gaatatgctt
     1861 acgcgaagca gaacaacctg gcgtacacca aactgatctc cgctgatggt aaaccggtta
     1921 gtccgaccga agaaaacttc gctaatgcag caaaaggtgc agactggagc aaaaccttcg
     1981 ctcaggatct gaccaaccag aaaggcgaag atgcatggcc tattacctct accacgttca
     2041 ttctgatcca caaagatcag aagaaaccag aacaaggcac agaggtgctg aaattcttcg
     2101 actgggcgta caaaaccggg gctaaacagg cgaacgacct ggattacgcc agcctgccgg
     2161 atagtgtagt tgaacaggtt cgcgctgcgt ggaagaccaa tattaaagac agtagcggta
     2221 agccgctgta ctaataaaac tccaggccgg gtacggtgtt ttacgccgca tccggcatta
     2281 caaaatgact ttgtaaacgc gtttaactga agagtaactt atggctgcaa ccaagcccgc
     2341 ttttaaccca ccgggtaaaa agggcgacat aattttcagc gtgctggtaa aactggcggc
     2401 gctgattgtg ctattgatgt tgggtggcat tattgtctct ctgatcatct cctcctggcc
     2461 gagcattcag aaatttggtc tggctttcct atggaccaaa gagtgggatg caccgaacga
     2521 tatctacggg gcgctggtgc cgatctacgg tacgttggtg acttcgttta tcgcgctgct
     2581 gatcgccgtc ccggtgagtt tcggtatcgc cctgttcctg actgagcttg cgcctggctg
     2641 gctgaaacgc ccgctgggta tcgccattga gctgctggca gccattccaa gtatcgttta
     2701 cggcatgtgg ggcctgttta tctttgcgcc gctgttcgcc gtttactttc aggagccggt
     2761 cggcaatatc atgtcgaata tcccgattgt tggcgcgctg ttctctggcc ccgcatttgg
     2821 tatcggtatc ctcgcggcag gcgtgatcct cgccatcatg attattccgt acattgcggc
     2881 ggtaatgcgt gatgtgttcg aacaaacccc ggtgatgatg aaagagtcgg cctacggtat
     2941 tggctgcacc acctgggaag ttatctggcg tatcgttctt ccgttcacca aaaatggtgt
     3001 tatcggcggc atcatgctgg ggctgggccg cgcgctcggt gaaaccatgg cggtgacctt
     3061 tatcatcggt aacacctacc agctcgacag cgcctcgctg tacatgccgg gcaatagtat
     3121 cacctctgcg ctggcgaacg aatttgcgga agcggaatcc ggtctgcacg ttgccgcact
     3181 gatggaactg ggcctgatcc tgtttgtgat taccttcatc gtcctcgccg catcgaagtt
     3241 tatgattatg cgcctggcta agaatgaggg ggcacgctaa tggctatggt tgaaatgcaa
     3301 accactgcgg cgctggctga atctcgccgc aaaatgcagg cgcgtcgccg cctcaaaaac
     3361 cgtattgcgc tgacgctctc gatggcgacg atggccttcg gcctgttctg gctgatctgg
     3421 attttaatgt ccaccatcac tcgcggtatc gacggtatgt cgctggcgct gttcactgaa
     3481 atgacgccgc cgcccaatac ggaaggtggt ggtctggcga acgccctggc gggtagcggg
     3541 ctgttaattt tgtgggccac ggtattcggt acgccgctgg gcattatggc ggggatttat
     3601 ctggcggaat atggtcgtaa atcctggctg gcagaagtga ttcgcttcat taacgacatt
     3661 ctgctctccg cgccgtcgat tgtggttggt ctgtttgtct acaccatcgt ggtggcacag
     3721 atggagcact tctccggctg ggcgggcgta attgcgctgg cgctgttgca ggtgccgatc
     3781 gttatccgca ccactgagaa catgctgaaa ctggtgccgg acagcctgcg tgaagcggct
     3841 tatgcgctgg gtacaccgaa gtggaagatg atctccgcga ttacgctgaa agcgtcggtg
     3901 tccgggatta tgaccggtat cctgctggcg attgcccgta ttgccggtga aaccgcgccg
     3961 ctgctgttta ccgcgctctc caaccagttc tggagcacgg acatgatgca gccgatcgcc
     4021 aacctgccgg tgacgatctt taagtttgcg atgagcccgt ttgcggaatg gcaacaactg
     4081 gcctgggccg gggtattgat cattaccctg tgcgtcctgc tgctgaacat tctggcgcgc
     4141 gttgtttttg cgaagaataa acacggttaa cacttttccg gatgcggcgt gaacgcctta
     4201 cccggcccta cggtaagcct gattagcgaa aagcatcagg tacgatgagg aaaagattgc
     4261 aatgagtatg gttgaaactg ccccgagtaa aattcaggtt cgtaatttga acttctacta
     4321 cggcaaattc catgccctga aaaacatcaa cctggatatc gccaaaaacc aggttacggc
     4381 gtttatcggg ccgtccggct gcggtaaatc gacgctgctg cgtaccttca acaaaatgtt
     4441 tgaactgtac ccggagcagc gtgcggaagg tgaaattctg cttgatggcg acaacatcct
     4501 gaccaactct caggatatcg ccctgctgcg tgcgaaagtg ggcatggtgt tccagaaacc
     4561 gacgccgttc ccgatgtcca tctacgacaa catcgccttt ggcgttcgtc tgtttgagaa
     4621 gctctcccgt gccgacatgg acgagcgcgt gcagtgggca ttgaccaaag ccgcattgtg
     4681 gaacgaaacc aaagataaat tgcaccagag cggttactct ctctctggtg gtcagcaaca
     4741 gcgtctgtgt attgcgcgtg gtatcgccat tcgcccggaa gtgctgctgc tcgacgaacc
     4801 gtgttcggcg ctcgacccca tctctaccgg gcgtattgaa gagctgatca ccgaactgaa
     4861 gcaggattac accgtggtga tcgtcaccca caacatgcag caggctgcgc gttgttccga
     4921 ccacacggca tttatgtacc tgggcgaatt gattgagttc agcaacacgg acgatctgtt
     4981 caccaagcca gcgaagaaac aaacagaaga ctacatcacc ggtcgttacg gttgattcag
     5041 gagtgcgtta tggacagtct caatcttaat aaacatattt ccggccagtt caacgccgaa
     5101 ctggaaagta tccgcacgca ggtgatgacc atgggcggca tggtggagca gcagctttct
     5161 gatgcaatca ccgcgatgca taaccaggac agcgatctgg cgaagcgcgt gatcgaaggc
     5221 gacaagaacg tcaacatgat ggaagtggcg atcgatgaag cctgcgtgcg cattatcgcc
     5281 aaacgtcagc cgacggcgag cgacctgcga ctggttatgg tgatcagtaa aaccattgcc
     5341 gagctggagc gtattggcga cgtggcggac aaaatctgcc gtactgcgct ggagaaattc
     5401 tcccagcagc atcagccgtt gctggtaagt ctggagtcgc tgggccgtca taccatccag
     5461 atgctgcacg acgtgctgga cgcgttcgcg cggatggaca ttgacgaagc ggtacgtatt
     5521 tatcgtgaag ataaaaaagt cgatcaggaa tacgaaggta ttgttcgtca actgatgacc
     5581 tacatgatgg aagattcgcg cactattccg agcgtactta ccgcgctgtt ctgtgcgcgt
     5641 tcaatcgaac gtatcggcga ccgctgccag aatatttgtg agtttatctt ctactacgtg
     5701 aagggtcagg atttccgtca tgtaggcggt gatgagctgg ataaactgct ggcgggaaaa
     5761 gatagcgaca aataattcac cagacaaatc ccaataactt
//
LOCUS       DAZXUL010000195         5744 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZXUL010000195 DAZXUL010000000
VERSION     DAZXUL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.196"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..173
                     /locus_tag="ACH3L1_004064"
                     /pseudo
     CDS             1..173
                     /locus_tag="ACH3L1_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(489..827)
                     /locus_tag="ACH3L1_004065"
     CDS             complement(489..827)
                     /locus_tag="ACH3L1_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078574.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            749..1876
                     /gene="ltrA"
                     /locus_tag="ACH3L1_004066"
     CDS             749..1876
                     /gene="ltrA"
                     /locus_tag="ACH3L1_004066"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFT4078575.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFNGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            1959..2138
                     /locus_tag="ACH3L1_004067"
                     /pseudo
     CDS             1959..2138
                     /locus_tag="ACH3L1_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2165..2650)
                     /locus_tag="ACH3L1_004068"
     CDS             complement(2165..2650)
                     /locus_tag="ACH3L1_004068"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFT4078576.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2906..3886)
                     /locus_tag="ACH3L1_004069"
     CDS             complement(2906..3886)
                     /locus_tag="ACH3L1_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFT4078577.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3886..5085)
                     /locus_tag="ACH3L1_004070"
     CDS             complement(3886..5085)
                     /locus_tag="ACH3L1_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFT4078578.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5259..5393)
                     /locus_tag="ACH3L1_004071"
                     /pseudo
     CDS             complement(5259..5393)
                     /locus_tag="ACH3L1_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5407..>5744)
                     /locus_tag="ACH3L1_004072"
     CDS             complement(5407..>5744)
                     /locus_tag="ACH3L1_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFT4078579.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1574 a         1317 c         1368 g         1485 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg
      361 aagcatcgaa atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc
      421 agaagacacc tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc
      481 agaccatctc agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac
      541 ccattcgtag tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca
      601 ttcattgcag aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt
      661 gaaataccaa aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc
      721 ccccggatgc gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata
      781 caagatctgg aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg
      841 gatcccgaag tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat
      901 tgctcaggga gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga
      961 ttcatatggt taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg
     1021 gtgctggcgt tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag
     1081 acatgatctg gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta
     1141 ctgtcgacgc tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg
     1201 aacacgaggc actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca
     1261 ctatgcattt gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc
     1321 tgacgatatt gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt
     1381 atcggagcgc ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta
     1441 cattgacacg tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt
     1501 caaagtgcgt acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc
     1561 gtcaaatgca gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc
     1621 aacagctgag agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat
     1681 cgagtactac ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca
     1741 gtcacgtctg ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg
     1801 aaagctgacg ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg
     1861 tgcatcaaat gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag
     1921 cagccgggga gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg
     1981 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag
     2041 aaaaatggct gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg
     2101 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag
     2161 ccgcttactt ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa
     2221 gaagtccacg aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat
     2281 tcaaaaaagt ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc
     2341 catgttggca ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct
     2401 gataagcttt aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt
     2461 cccgggccca gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt
     2521 gcaccagcca gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat
     2581 gagcattgat actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct
     2641 gttcagacat ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg
     2701 aaggctgccg tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga
     2761 aactatcttt ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg
     2821 ttcaaaaaaa acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg
     2881 agttgctggc gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag
     2941 caatcatacg atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga
     3001 acgtacgtcc ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca
     3061 aagttacaac cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta
     3121 taagccgcat aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa
     3181 gctcaatact cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca
     3241 gtcgttcact taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag
     3301 gaaaaagaga cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag
     3361 ctgccgacaa tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac
     3421 gtactaaacg caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg
     3481 ccagatgctg agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa
     3541 ccttgcatag caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc
     3601 cagtccgaat cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt
     3661 ttagtgagtc cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag
     3721 tttgagtatc cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag
     3781 tattgagggt aaatacatga actggagccg atatttcttc agtattattc aggatatttg
     3841 tattcagcgt acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct
     3901 tataaattca atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt
     3961 tttcaaagct tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa
     4021 cgactcgccg cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc
     4081 accgaaaatc tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt
     4141 cgacatgaag ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg
     4201 tacaagttca ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg
     4261 cggcaccggt gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag
     4321 atgcgggcct gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt
     4381 ctcacgcaat atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca
     4441 gtttgatgca ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac
     4501 tgacggaacg ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg
     4561 cggtgcggtt gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga
     4621 tgactgggga tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg
     4681 agcacgcagg gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag
     4741 actaccgatg aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg
     4801 atgtgcatag atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc
     4861 tgggcgtttt tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct
     4921 tgcgcgcgtt aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac
     4981 atcagcctga aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt
     5041 atctgcacgc tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta
     5101 tgtaggattt taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca
     5161 tattgtacat gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa
     5221 tattactgta agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc
     5281 agttcaccac cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg
     5341 gaaacatgtt ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc
     5401 ttccaatcag tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc
     5461 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
     5521 ccggttgtta cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg
     5581 cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt
     5641 caacagggct aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa
     5701 cacaccgcca gttttagtgt tgtacgcata ccagagccag tgtt
//
LOCUS       DAZXUL010000196         5738 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZXUL010000196 DAZXUL010000000
VERSION     DAZXUL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.197"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..241
                     /locus_tag="ACH3L1_004073"
     CDS             <1..241
                     /locus_tag="ACH3L1_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078580.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="ACH3L1_004074"
     CDS             complement(238..1179)
                     /locus_tag="ACH3L1_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFT4078581.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="ACH3L1_004075"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="ACH3L1_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="ACH3L1_004076"
     CDS             complement(1684..2904)
                     /locus_tag="ACH3L1_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFT4078582.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="ACH3L1_004077"
     CDS             complement(2908..3651)
                     /locus_tag="ACH3L1_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFT4078583.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="ACH3L1_004078"
     CDS             complement(3542..5008)
                     /locus_tag="ACH3L1_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFT4078584.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQGRRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5738)
                     /locus_tag="ACH3L1_004079"
                     /pseudo
     CDS             complement(5008..5738)
                     /locus_tag="ACH3L1_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1226 a         1619 c         1435 g         1458 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tcccctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAZXUL010000197         5702 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZXUL010000197 DAZXUL010000000
VERSION     DAZXUL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.198"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..170)
                     /locus_tag="ACH3L1_004080"
     CDS             complement(<1..170)
                     /locus_tag="ACH3L1_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078585.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="ACH3L1_004081"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="ACH3L1_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFT4078586.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="ACH3L1_004082"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="ACH3L1_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACH3L1_004083"
     CDS             complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACH3L1_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFT4078587.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /gene="ltnD"
                     /locus_tag="ACH3L1_004084"
                     /pseudo
     CDS             4579..5487
                     /gene="ltnD"
                     /locus_tag="ACH3L1_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            5484..>5702
                     /locus_tag="ACH3L1_004085"
     CDS             5484..>5702
                     /locus_tag="ACH3L1_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFT4078588.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAZXUL010000198         5655 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZXUL010000198 DAZXUL010000000
VERSION     DAZXUL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.199"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..340
                     /locus_tag="ACH3L1_004086"
     CDS             <1..340
                     /locus_tag="ACH3L1_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HFT4078589.1"
                     /translation="LLGKTLGNKGIGLEGGGIFTVGDECHAHFLVEQGKEAKIALEQA
                     GLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYSDHANQLILITDNDSMA
                     ASVTLPWAIK"
     gene            348..1034
                     /locus_tag="ACH3L1_004087"
     CDS             348..1034
                     /locus_tag="ACH3L1_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFT4078590.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1012..1635)
                     /gene="tetR(B)"
                     /locus_tag="ACH3L1_004088"
     CDS             complement(1012..1635)
                     /gene="tetR(B)"
                     /locus_tag="ACH3L1_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFT4078591.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1717..2922
                     /gene="tet(B)"
                     /locus_tag="ACH3L1_004089"
     CDS             1717..2922
                     /gene="tet(B)"
                     /locus_tag="ACH3L1_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFT4078592.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3035..3628)
                     /gene="tetC"
                     /locus_tag="ACH3L1_004090"
     CDS             complement(3035..3628)
                     /gene="tetC"
                     /locus_tag="ACH3L1_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFT4078593.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3716..4132
                     /locus_tag="ACH3L1_004091"
     CDS             3716..4132
                     /locus_tag="ACH3L1_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4078594.1"
                     /translation="MYIEQHSRYQNKANNIQLRYDDKQFHTTVIKDVLLWIEHNLDQS
                     LLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALK
                     YQFDSQQSFTRRFKYIFKVTPSYYRRNKLWELEAMH"
     gene            complement(4142..5272)
                     /locus_tag="ACH3L1_004092"
     CDS             complement(4142..5272)
                     /locus_tag="ACH3L1_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFT4078595.1"
                     /translation="MGEISVKTGNNARNGGKNSLNRLIRCVYGKKIGPDPRNFNQRVS
                     LGRNDLLIRTFPSFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGRSVTLYEKAF
                     PLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGWYWLSRVRGK
                     VQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSRSKGRKNQRS
                     TRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEETFRDLKSPA
                     YGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQANTVRNRNVLS
                     TVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
     gene            complement(5254..>5655)
                     /locus_tag="ACH3L1_004093"
     CDS             complement(5254..>5655)
                     /locus_tag="ACH3L1_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HFT4078596.1"
                     /translation="VTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDV
                     DWLIAERPGKVKTLKQHPRKNKTVINIEYMKASIRARVEHPFRIIKRQFGFVKARYKG
                     LLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
BASE COUNT         1502 a         1106 c         1245 g         1802 t
ORIGIN      
        1 attactagga aaaacattag gtaataaagg tattggattg gaagggggag ggatatttac
       61 ggttggtgat gaatgccatg ctcattttct tgttgaacaa ggaaaggaag ctaaaatagc
      121 gctagagcaa gctggactgt tagtacttgc gatccggaca ccattaattc gtaagttaaa
      181 acaggaaaaa ccgggggaac ttggcgaaat agcacgagta ttggcggaga ataacattaa
      241 tattttagtg caatacagtg accatgctaa ccaactgata ttaataacgg acaatgatag
      301 tatggctgca tctgttacgc tcccttgggc aataaagtga acttgcgatg gctaatttaa
      361 tacgaaaaga ggttaccttt gagtcctcaa tagccgcgat aggggcggct atgtctgaca
      421 tttcacgagt taaaatactc agtgctttga tggatgggcg agcttggacg gccactgagc
      481 taagttctgt ggcgaatata tcagcttcaa cggcgagcag tcatttatct aaattattag
      541 attgccagct aatcacagta gtagctcaag gcaagcatcg ttattttcgg ctagcaggaa
      601 aagatattgc tgaattgatg gaaagtatga tggggatctc cttaaaccat ggcgtacatg
      661 ccaaagtttc cacgccagtg catttacgaa aagcacgtac ttgctatgat catttagctg
      721 gcgaagttgc cgttaagatc tatgattccc tttgtcaaca gcaatggatc actgaaaatg
      781 gttcaatgat cacattaagt ggtattcaat attttcatga aatgggaatt gacgttcctt
      841 ccaaacattc acgtaaaatc tgttgtgcgt gtttagattg gagtgaacgc cgtttccatt
      901 taggtgggta cgttggagcc gcattatttt cgctttatga atctaaaggg tggttaactc
      961 gacatcttgg ttaccgtgaa gttaccatca cggaaaaagg ttatgctgct tttaagaccc
     1021 actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa
     1081 ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat
     1141 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa
     1201 tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga
     1261 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt
     1321 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
     1381 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
     1441 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt
     1501 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
     1561 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt
     1621 atctaatcta gacatcatta attcctaatt tttgttgaca ctctatcatt gatagagtta
     1681 ttttaccact ccctatcagt gatagagaaa agtgaaatga atagttcgac aaagatcgca
     1741 ttggtaatta cgttactcga tgccatgggg attggcctta tcatgccagt cttgccaacg
     1801 ttattacgtg aatttattgc ttcggaagat atcgctaacc actttggcgt attgcttgca
     1861 ctttatgcgt taatgcaggt tatctttgct ccttggcttg gaaaaatgtc tgaccgattt
     1921 ggtcggcgcc cagtgctgtt gttgtcatta ataggcgcat cgctggatta cttattgctg
     1981 gctttttcaa gtgcgctttg gatgctgtat ttaggccgtt tgctttcagg gatcacagga
     2041 gctactgggg ctgtcgcggc atcggtcatt gccgatacca cctcagcttc tcaacgcgtg
     2101 aagtggttcg gttggttagg ggcaagtttt gggcttggtt taatagcggg gcctattatt
     2161 ggtggttttg caggagagat ttcaccgcat agtccctttt ttatcgctgc gttgctaaat
     2221 attgtcgctt tccttgtggt tatgttttgg ttccgtgaaa ccaaaaatac acgtgataat
     2281 acagataccg aagtaggggt tgagacgcaa tcgaattcgg tatacatcac tttatttaaa
     2341 acgatgccca ttttgttgat tatttatttt tcagcgcaat tgataggcca aattcccgca
     2401 acggtgtggg tgctatttac cgaaaatcgt tttggatgga atagcatgat ggttggcttt
     2461 tcattagcgg gtcttggtct tttacactca gtattccaag cctttgtggc aggaagaata
     2521 gccactaaat ggggcgaaaa aacggcagta ctgctcggat ttattgcaga tagtagtgca
     2581 tttgcctttt tagcgtttat atctgaaggt tggttagttt tccctgtttt aattttattg
     2641 gctggtggtg ggatcgcttt acctgcatta cagggagtga tgtctatcca aacaaagagt
     2701 catcagcaag gtgctttaca gggattattg gtgagcctta ccaatgcaac cggtgttatt
     2761 ggcccattac tgtttgctgt tatttataat cattcactac caatttggga tggctggatt
     2821 tggattattg gtttagcgtt ttactgtatt attatcctgc tatcgatgac cttcatgtta
     2881 acccctcaag ctcaggggag taaacaggag acaagtgctt agttatttcg tcaccaaatg
     2941 atgttattcc gcgaaatata atgaccctct tgataaccca agagggcatt ttttacgata
     3001 aagaagattt agcttcaaat aaaacctatc tattttattt atctttcaag ctcaataaaa
     3061 agccgcggta aatagcaata aattggcctt ttttatcggc aagctctttt aggtttttcg
     3121 catgtattgc gatatgcata aaccagccat tgagtaagtt tttaagcaca tcatcatcat
     3181 aagctttaag ttggttctct tggatcaatt tgctgacaat ggcgtttacc ttaccagtaa
     3241 tgtattcaag gctaattttt tcaagttcat tccaaccaat gataggcatc acttcttgga
     3301 tagggataag gtttttatta ttatcaataa tataatcaag ataatgttca aatatacttt
     3361 ctaaggcaga ccaaccattt gttaaatcag tttttgttgt gatgtaggca tcaatcataa
     3421 ttaattgctg cttataacag gcactgagta attgtttttt atttttaaag tgatgataaa
     3481 aggcaccttt ggtcaccaac gcttttcccg agatctcatc tattgaaaca gcttgatagc
     3541 ctttttcaac aaacaatatt cgtgctgagt taaccagtga ttgataggta ctcttaaaat
     3601 tttcttgttg atgattttta ttttccatga tagatttaaa ataacatacc gtcagtatgt
     3661 ttatggtatc atgatgatgt ggtcgtgaca atcttaagaa catttaggtt attttatgta
     3721 tattgaacag cattctcgct atcaaaataa agctaataac atccaattaa gatatgatga
     3781 taagcagttt catacaacgg ttatcaaaga tgttctatta tggattgaac ataatttaga
     3841 tcagtcttta ctgcttgatg atgtggcgaa taaagcgggt tataccaagt ggtattttca
     3901 gcggctgttc aaaaaagtaa caggggtcac actggctagc tatattcgtg ctcgtcgttt
     3961 gacgaaagcg gctgttgagt tgaggttgac gaaaaaaact atccttgaga tcgcattaaa
     4021 atatcaattt gattcccaac aatcttttac acgtcgattt aagtacattt ttaaggttac
     4081 accaagttat tatcggcgta ataaattatg ggaattggag gcaatgcact gagagatccc
     4141 ctcataattt ccccaaagcg taaccatgtg tgaataaatt ttgagctagt agggttgcag
     4201 ccacgagtaa gtcttccctt gttattgtgt agccagaatg ccgcaaaact tccatgccta
     4261 agcgaactgt tgagagtacg tttcgatttc tgactgtgtt agcctggaag tgcttgtccc
     4321 aaccttgttt ctgagcatga acgcccgcaa gccaacatgt tagttgaagc atcagggcga
     4381 ttagcagcat gatatcaaaa cgctctgagc tgctcgttcg gctatggcgt aggcctagtc
     4441 cgtaggcagg acttttcaag tctcggaagg tttcttcaat ctgcattcgc ttcgaataga
     4501 tattaacaag ttgtttgggt gttcgaattt caacaggtaa gttagttgct agaacccatg
     4561 gctcctttgc cgacgctgag tagattttag gtgacgggtg gtgacaatga gtccgtgtcg
     4621 agcgctgatt ttttcggcct ttagagcgag atttatacaa tagaatttgg catgagattg
     4681 gattgctttt agtcagcctc ttatagccta aagtctttga gtgactagat gacatatcat
     4741 gtaagttgct gataggtttc cagttttccg ctcctaggtc tgcatattgt acttttcctc
     4801 ttactcgact taaccagtac caacccagct tctcaacgga tttataccat ggcactttaa
     4861 agccagcatc actgacaatg agcggtgtgg tgttactcgg tagaatgctc gcaaggtcgg
     4921 ctagaaattg gtcatgagct ttctttgaac attgctctga aagcgggaac gctttctcat
     4981 aaagagtaac agaacgaccg tgtagtgcga ctgaagctcg caataccata agtcgttttt
     5041 gctcacgaat atcagaccag tcaacaagta caatgggcat cgtattgccc gaacagataa
     5101 agctagggaa ggtgcgaata agcaggtcat ttcttcccaa gctgactcgc tgattaaaat
     5161 ttcgcggatc tgggccgatt tttttcccgt aaacacatcg aatcagccta tttaggctat
     5221 tttttccacc atttctggcg ttatttccgg tttttactga gatctctccc actggcgtat
     5281 catttggtcc acccgaaaca ggttggccag ggtgaataac atcgccagtt ggttatcgtt
     5341 tttcagcagc cccttgtatc tggctttcac gaagccgaac tgccgcttga tgatgcgaaa
     5401 cgggtgctcc accctggcac ggatgctggc tttcatgtat tcgatgttga tgaccgtttt
     5461 gttcttgcgc ggatgctgct tcaaggtttt taccttgccg ggacgctcgg cgatcagcca
     5521 gtccacatcc acctcggcca gctcctcgcg ctgtggcgct ccttggtagc cggcatcggc
     5581 tgagacaaat tgctcctctc catgaagcag attacccagc tgattgaggt catgctcgtt
     5641 ggccgcggtg gtgac
//
LOCUS       DAZXUL010000199         5644 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZXUL010000199 DAZXUL010000000
VERSION     DAZXUL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.200"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            753..1700
                     /gene="icsP"
                     /locus_tag="ACH3L1_004094"
     CDS             753..1700
                     /gene="icsP"
                     /locus_tag="ACH3L1_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFT4078597.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2262..2634)
                     /locus_tag="ACH3L1_004095"
                     /pseudo
     CDS             complement(2262..2634)
                     /locus_tag="ACH3L1_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            2878..2997
                     /locus_tag="ACH3L1_004096"
                     /pseudo
     CDS             2878..2997
                     /locus_tag="ACH3L1_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            3247..4113
                     /gene="ospB"
                     /locus_tag="ACH3L1_004097"
     CDS             3247..4113
                     /gene="ospB"
                     /locus_tag="ACH3L1_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFT4078598.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            4442..5182
                     /gene="phoN2"
                     /locus_tag="ACH3L1_004098"
     CDS             4442..5182
                     /gene="phoN2"
                     /locus_tag="ACH3L1_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFT4078599.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            5255..5377
                     /locus_tag="ACH3L1_004099"
     CDS             5255..5377
                     /locus_tag="ACH3L1_004099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078600.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(5542..5628)
                     /locus_tag="ACH3L1_004100"
     CDS             complement(5542..5628)
                     /locus_tag="ACH3L1_004100"
                     /inference="COORDINATES: protein motif:HMM:NF017898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078601.1"
                     /translation="MLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         1769 a         1065 c         1131 g         1679 t
ORIGIN      
        1 tataaaagca ccaatgaatt gtccaagtaa acatgagata aaatgtgtag aggataactg
       61 agtttggtca ctttaactgt attagtcgca gtctgactct cgactttaaa aggatgggaa
      121 ttacctgtac tgatagtgag aaagctttcc attgaaataa gtcaaataaa agttgatggc
      181 ttcagtgcta aagtgaggtg cccgctagcc gtattcactc gaatcggaat tcaatgtatt
      241 gcttctgcca tttccataat atttcacggg aaaaacaaaa tctgaaaaac tatacttaag
      301 tgttgtattg agtcagttga gatacaaata catatttatc agcacacgtt acgtcaaata
      361 caggaggttg tcatgttggt actgaaaggc acgttggcta ttgcagccgt gatgacaatt
      421 tattgtctta tcgttgttct tatggaacgc ttttctgatt gatttcatat tggcgagaga
      481 acaagaaggt gagtagaatt gtcgcggaat cctgaaacta tcagcctcag gtatgaacac
      541 caaaaggtag gtagagaaat ctatggcccc cgttacttta ttcgcacaat gaggtatttt
      601 tttattttta caaaagttca ataaatatta taataaaaga tatgttcttg gtaacataaa
      661 caaatattta attaaaataa tgcttggcat tgacgtttat cttataaagt aagaagatca
      721 tggacatttc aaccaaaaaa gtagagttct cgatgaaatt aaaattcttt gtacttgcac
      781 tttgtgtacc tgcgatcttt actacacatg ctaccactaa ctatccactt ttcataccgg
      841 acaacatcag tacggatatt agtctaggat ctctgagtgg caaaacaaaa gaacgcgttt
      901 atcatcccaa ggaagggggg agaaaaatta gtcaactgga ctggaaatac agtaatgcga
      961 ctattgttag aggtggcatc gattggaagc taattccaaa agtgtctttc ggagtttccg
     1021 gttggactac tttaggtaac cagaaagcaa gcatggttga taaagactgg aacaattcca
     1081 atacccctca ggtatggacc gaccagagct ggcatcccaa tacgcatctc cgtgatgcta
     1141 acgaattcga gctgaatctt aaaggttggt tattaaataa tttggattat cgacttggac
     1201 taatagcagg ttaccaagag agtcgttaca gttttaatgc aatgggaggg agttatattt
     1261 atagtgaaaa cggggggagc aggaataaaa aaggggcaca tcctagtggt gaaagaacaa
     1321 taggttacaa acagctcttc aaaatacctt atattggatt aactgctaat taccgccatg
     1381 agaattttga gtttggagca gaactgaaat atagtggttg ggttctttca tctgatacag
     1441 ataaacacta tcagactgag acaattttta aagatgaaat aaaaaaccaa aattactgct
     1501 ctgttgctgc gaatattgga tactatgtca cccccagtgc aaaattttat atagaaggct
     1561 ccagaaatta catttctaat aaaaaaggtg atacatctct ttatgagcaa agtaccaata
     1621 tatctggcac cattaaaaat agtgcaagta ttgaatatat tggttttctc acttccgcag
     1681 gtataaagta tattttttga attggttcac cgagttgatt acgttttcat ggctaaaaat
     1741 tgccgcccat tgtgagtcat attcgccctg actttccaga accatacgaa ctgcccgttg
     1801 acggacttcg ggggaaaaac gtgtattttt agtcattctg tttacctctt tctcagggcg
     1861 tttagtctcc aggatttccg gggcggttca aaaaactctt cgttgattac tgtggactta
     1921 ctgttggcat taccgaccct gagaccggag aaataagaac cgcttagatc atcgtggctg
     1981 ttctcggggt atcaagttac acatgggcag aggctaccca aggtctacta ttatcgcctg
     2041 aaagcgctga tggaacagtg ctatcaggga catgctgatg aaaataccta aataatcagc
     2101 cagcactcta tctttccaaa tccacagcat agcaaagaga gcaaaagagc ctgtaaattc
     2161 agaaattaag gaaacgcctc ttggtgtaca taatctatct tatgttaaat gaaatctaat
     2221 ttcttgatta tatgctggat cgttcgtggg tggctttgcc cctatatttc cagacacctg
     2281 ttatcattgg cccgctgccg cagatattcc cgtggcgagc gataatccag cgcactatgc
     2341 ggatgccatt cgttataatg ctcgaatgcc tctgcaaggt tctttgctgc cgttaacccg
     2401 tcaggtttgg gcatgatact gatgtagtca cactttattg ttttcacgaa gctctttgct
     2461 attctgttac tctccggact ccgcaccgcc gtgttcttcg gttcaacaaa gtcccaacat
     2521 ccgggtgaac tggcgtgttt cattagctcg gtagcatgaa ccattatccg tcagtcactt
     2581 cactggagac gccggaagct cgttgccgaa gcggtgttcc accgctccca gcatgtcgcc
     2641 aacgggagag catctacggt ctgattgaac tgtttattca aattgtccac cgtctgtcag
     2701 tacgctctga acgtcggctg gttaaaacac tgctcgctga ttttcagagg gttcacggta
     2761 aaacagcact tttattccgg attgcggaag cggctctgaa taatccggat gggctggtta
     2821 aagaggttgt gtaccatctg catattgttg aaccagatcg cagcggtaag agaagttcat
     2881 catacttggc gcagttacgc gatgtttctg ctagaggtga tgccgtaaag aacggcagaa
     2941 cattaccgga acaagatagc ggtctacctg cactggtatc agacccgggg cttccccgga
     3001 tgatctccac ggtactttag gtactgtgtc tatattatgt aggtaggatc aattaacttt
     3061 gtagttgaaa ttctttcatt gaaataatga ataaaaaaga caaaccgcat taaacaatgc
     3121 cagaatataa ttgcattttt gttccgtttt tttaaatcat agtatacaaa gaagcttgca
     3181 tactatatga aaatataaca ccctttacta tgatgtttat aaaacaatat atggagtcat
     3241 gtaggtatga atttagatgg tgttagacca tactgtagaa tagtcaataa aaagaatgaa
     3301 agcatatcag atattgcatt tgcacatata ataaaaaggg taaaaaattc atcatgtact
     3361 cacccaaaag cagcattggt ttttttagga gagaaaggtt tttgtgatag caatgatgtt
     3421 ctatctatta tgggacaaca aataccaaga gtatttaaga acaagatgtt atatgattat
     3481 gtttttaaaa atgaaaaaag taaaaatgat tttctaaaaa tggctgaatc atggctacca
     3541 cagagtgaac caatagtaat aaataatgat gatgacgcat tgaatgctgc tgcttatttt
     3601 tctgtaaaaa aagcgaaaat aaaaacagta aacgatactg attttaaaga gtataataag
     3661 gtttatattc ttgggcacgg tagtcctggt tctcatcaat taggccttgg ttcggaactt
     3721 attgatgtac aaacaatcat ttcaagaatg aaagactgtg gtattctaaa tgtgaaagat
     3781 atccgtttta cttcatgcgg ctccgctgat aaagtggctc ctaaaaattt taacaatgcc
     3841 cctgctgaaa gtctttcttg tatccttaac tctctgcctt tttttaagga aaaagaatct
     3901 ttgctagagc agataaaaaa acaccttgaa aacgatgagt cattgagtga tggtctaaaa
     3961 atatccggct atcatggata tggagttcac tatggtcaag agctttttcc ctactcacat
     4021 tatcgttcaa cttcaattcc tgctgatccg gagcatacag taaaaagaag ctctcagaaa
     4081 aagactttta ttattaataa agaactggat tagtataaaa tttttaacct atagttaatt
     4141 gtttgcattt aagatgaagt tattttatct ttagtagaaa agtgcttccc tatgagggaa
     4201 aatcacaaat catcataata cagagacaaa acgatacgaa aaaatagata aaaaacatca
     4261 ttcttttacc acattatttt ccgtgaatat gaaaaataat gttattactt taatataaga
     4321 ctattttttg tttttccatc actctgttca aatttttccg catgacttgt gttttttgta
     4381 atacagctcg ttttttacag ctgaccaaaa tcatcaatta attatgctaa ggaaataaat
     4441 tatgaaaacc aaaaactttc ttcttttttg tattgctaca aatatgattt ttatcccctc
     4501 agcaaatgct ctgaaggcag aaggttttct cactcaacaa acttcaccag acagtttgtc
     4561 aatacttccg ccgcctccgg cagagaattc agtagtattt caggctgaca aagctcatta
     4621 tgaattcggc cgctcgctcc gggatgctaa tcgtgtacgt ctcgctagcg aagatgcata
     4681 ctacgagaat tttggtcttg cattttcaga tgcttatggc atggatattt caagggaaaa
     4741 taccccaatc ttatatcagt tgttaacaca agtactacag gatagccatg attacgccgt
     4801 gcgtaacgcc aaagaatatt ataaaagagt tcgtccattc gttatttata aagacgcaac
     4861 ctgtacacct gataaagatg agaaaatggc tatcactggc tcttatccct ctggtcatgc
     4921 atcctttggt tgggcagtag cactgatact tgcggagatt aatcctcaac gtaaagcgga
     4981 aatacttcga cgtggatatg agtttggaga aagtcgggtc atctgcggtg cgcattggca
     5041 aagcgatgta gaggctgggc gtttaatggg agcatcggtt gttgcagtac ttcataatac
     5101 acctgaattt accaaaagcc ttagcgaagc caaaaaagag tttgaagaat taaatactcc
     5161 taccaatgaa ctgaccccat aaagctggac agcctgtatc aggctatgga gggcccatag
     5221 acaaatctac cctatatgag cataggagga gtctatgggc acaccacgtt ttacccctga
     5281 atttaaggga ttactggaaa ggctgggaca tatccctccg gcagaagcag aaaaagctta
     5341 ttatgctgcc atcggaaacg atgatctggc aacctgagtt cacagataaa acattctcta
     5401 ggaaactcgg ggcggttccg ttcaccacat gcaatgtggt gttgcagggg aacggtctgc
     5461 accatcccct atgtcgatca atataacaga aatgacaact tcagattcag ggcacaacct
     5521 aaatatattt taggtcacct ctcaaatcgt ttgcctgata ccgctccatt ttttaataaa
     5581 aaaatcaatc atttttgaat ctttgctttt cattgcattg tcaagcatag tgtccccact
     5641 attt
//
LOCUS       DAZXUL010000200         5557 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZXUL010000200 DAZXUL010000000
VERSION     DAZXUL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.201"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..641
                     /locus_tag="ACH3L1_004101"
     CDS             <1..641
                     /locus_tag="ACH3L1_004101"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HFT4078602.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="ACH3L1_004102"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="ACH3L1_004102"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFT4078603.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="ACH3L1_004103"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="ACH3L1_004103"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFT4078604.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1538 g         1283 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct ctcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagatatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAZXUL010000201         5530 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZXUL010000201 DAZXUL010000000
VERSION     DAZXUL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.202"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            257..1255
                     /locus_tag="ACH3L1_004104"
                     /pseudo
     CDS             257..1255
                     /locus_tag="ACH3L1_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(1342..1818)
                     /locus_tag="ACH3L1_004105"
     CDS             complement(1342..1818)
                     /locus_tag="ACH3L1_004105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078605.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(1811..2575)
                     /locus_tag="ACH3L1_004106"
     CDS             complement(1811..2575)
                     /locus_tag="ACH3L1_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFT4078606.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            2821..3582
                     /locus_tag="ACH3L1_004107"
                     /pseudo
     CDS             2821..3582
                     /locus_tag="ACH3L1_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(3689..4201)
                     /locus_tag="ACH3L1_004108"
     CDS             complement(3689..4201)
                     /locus_tag="ACH3L1_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078607.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(4340..5488)
                     /locus_tag="ACH3L1_004109"
     CDS             complement(4340..5488)
                     /locus_tag="ACH3L1_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFT4078608.1"
                     /translation="MSITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQP
                     LGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDR
                     KLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAH
                     QSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDG
                     TLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTS
                     QLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDG
                     IAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT         1624 a         1194 c         1016 g         1696 t
ORIGIN      
        1 ggaagacaat tgtaaaaaaa aacaacagaa taagaattaa tcttaggata aatatttatt
       61 tatcatggcc ttttgaccgc taaaaaatgg cactcggcac agtaaacccc gctatagtta
      121 tgctatgcat ttgtctttaa tgatatctcg tcgttatatt aacaggctga aattatcatt
      181 aatgcacatt tcaatattag cagggatacc tgaggagttt aaagtgaaca tatatatcgg
      241 gtagcttttc aaattaatcc ctttgattat aggcttaatt tgcatcgcgt taggtggctt
      301 tgtgctggaa agttcagggc aacgtcacgg gtcatgtgct gatttctctg gcggccatat
      361 gcctggcatt attcactacc gcatttatta tcatttcgca actcacgcgc ggcgttaata
      421 cgttttacaa tacattgttc cccattattg gctatgcggg gtcaattatc accatgatat
      481 ggggttgggc actgttagca ggtaatgatg tgatggcaga cgagtttgtt gccggccatg
      541 ttatttttgg cgttggtatg attgccgcat gtgtatcgac ggtggcagcg tcgtccggtc
      601 actttctgct cattcccaaa aatgcagcgg ggagcaagag cgacggaaca ccggtacagg
      661 cttattcttc attaatcggt aattgcctca ttgccgttcc cgttttactc accctgctcg
      721 gtttcatttg gtctattacg ctgttacata gtgccgacat aactccgcat tatgtcgcct
      781 tgggttaacc gcaatctgtg cctgtctaat tggcctagtg gccacaatcg tccatcaaac
      841 acgtaatacg ttctcatcca aagagcactg gctgtggtgt tattgggtta tttttctcgg
      901 ctcagtcacg gtgctgcagg ggatatacgt cttagtcagt tccgatgcaa gcgcccgact
      961 ggctcccggc attattctca tttgcctcgg aatgatctgt tacagcatat tctcaaaagt
     1021 ctggctactg gcactggtct ggagacgtac ctgttcgtta gccaacagaa taccgatgat
     1081 accggtattt acctgtctgt tttgcttgtt tttgtcatca ttcctcgcgg aaatcgcaca
     1141 aactgagctg gactacttta ttccatctcg ggttctggtt ggattaggcg cagtatgttt
     1201 tacattgttc tctattgttt caatattaga ggccggttct gcgaaaaaaa attaaaattt
     1261 acataatccc agcgttattc atatcttaac gctggttttt tacgaactta gtgaaataat
     1321 gtagaatata aaattataat tttatattgg agcacatgtg ttaatgatca accccgggat
     1381 actaaaaata tagacattat taaacttgct tagcctcgca ttgcctttac ctgttaaaga
     1441 tgcatcgaga aaattgagtc tgaagttctc atcctttatc tgattttcca gtcccgaaac
     1501 tttggcctta gcattacctg agatccgtcc gttactatct attttgtagg taaaaaaata
     1561 cgaatgggaa aatttcctcg gtgtatcttc atcagttaag ccaacaatcg taaaactacc
     1621 gctaccatct ttttttaaaa tgactgaaag gttagcagag tagtataaag aatgatctgt
     1681 tttagcattt cttgttaacg tcgacgtaca ttcaagagac gttgtgctac catcacgagc
     1741 cttccagaga gcaaaaaaac acacctgtag caatgtgaaa acaagtatta atataaaata
     1801 cattttctta ttagtcatat aatagcacct ctgaacaaat tgcataagtt tcgtcattag
     1861 aggattttat acatcttgat aaataccgct gcccttcccc aagcaaataa tgcatactat
     1921 ttacatcata tataaaattt gcacctggca cacagggtaa atgctttttc gaaatttcag
     1981 cggcctctat ttgagattta gcgatgccaa tctcgttaag attctctgaa agatataaat
     2041 cacaggtgtc aatcttacct gatttaacca atggataatc cgttatactg taggtatctt
     2101 taaaagccac ggcaaacatt tcaataataa caattactac cacaagcgaa atcaaaatgc
     2161 gaaaaagagg aatactcctt ttgcgctcta ctttttcaac aacatcttct tgtaaatgag
     2221 aatcatcata agattgttta cttattgtta tgttttcact gatgtaaaag cctactttgg
     2281 gtatcgtaag aatcacattt tcttcaacac caatattttt aaaacttttg cgtaacaggg
     2341 atatatattg gttcaatgtg ttgtttgaca gatcaaatga aaatctctcc aaaacgctat
     2401 caatgattga gttcctgtca accgctccac cattactttc cagtaacact gccaaaatac
     2461 accccgccgt agctggaaaa tttatctcat cctgggtatt tacattcgag agcgagcatt
     2521 tgttcggatc aaaaataatc gcgtcattga taatgaaaaa acatctccgc tccatgcagg
     2581 caacaactca aaataagatt ttgtcatttt agcctattcc tggtggaact gacacaagaa
     2641 actaaaatac aaatataacc ctgaaaaagt catataaaac ttatatccac agaataaaaa
     2701 atcaaacatg tcagttttat caatataaag tatcgcaagg cgaaaagtga tgcaagaata
     2761 ctcctaagta aatgaaagcg caccacatga aattatcaac acatgtaatt tcatcattag
     2821 gtgctatcga aaataactca ggagctactt aagatgaata atgtcttact catgagaccc
     2881 gatgactcag aatttatgat acctgatgac tggtcagggc taaacgcaaa aggcattaca
     2941 tacgaacaag ataatagagt gacagagtct tttctgcctt ttaaatcttt ttattgttca
     3001 ccggtgtgca cgacagttga actcatctca taaaataatt gttttgctat gggtatccgc
     3061 taccttctgg aaaagaaaag agacttaagg ctaaatcact ttaaagcctt ctctcataaa
     3121 tgcgagacaa aacctgacgt gttaattatg accgggtgca ccgtcgctga tacttatagt
     3181 atattctcac gcattgttga agaaaatata atgaaaacga tgacacctat cgtcatcatt
     3241 actgggcatc actcagaaga tctttgttct ctggcatcga aactgggagg agttaaaata
     3301 atatcaccga cagaaaagat tgcaaaaata gaaaacgcta tcaatgatgc ttttaacagc
     3361 aagcctaaaa aaataagatt tttaacagat atactctctc ctcaacaaat tgagaccatg
     3421 aaaatggttg cgcagggtag atccgttaat gagatttcag ccgcaatgaa tattagcatc
     3481 aagaccgtgt atgcacataa agttcgtgct atcaataagc tgggaattaa gaaaaaagtt
     3541 tatgaagcgc ttttttataa agcgctaatt cagttcaact gatgtaaaaa tcaccctcgc
     3601 ttaataaata atatattcta tgtctttttt tataagttaa aaaaggcggg atatcaaatc
     3661 ccgccatgaa attaactaat catctgcatt attctgcgta gatataagcc tgggtacgga
     3721 tggtgtaaga gtctggctca acaacctgtt ctttcatatg ggaaacgacg cgataggcac
     3781 cacctgttcc agtatttttc agcatcatat aacgcttacc gccaatctct tcggaggcag
     3841 ctgaaggagg atcctgctca agcgttgtca gggaaacttt cagtttatgg tccgggttat
     3901 tgtcaccaga aattactgca tccgctgaag tactgctctc cgcataaata tcatccggga
     3961 aagttacagc atagtcgtgg ctctgcccat cagatgcgcc aacaatacct gttgccaaag
     4021 gcgtccatgc agttaacgta ccgctatata cccctttcac tggttgaatg gtattattaa
     4081 caacgatagg cttggtcaaa gtaagattag ctgacgcttc agtaccctgg cttactaaag
     4141 ttcccgcctg agaagcaaaa gctgtccccg ccagcatgat tgctaacaat gttttcttca
     4201 ttatattttc tccatgattt tcatttacac tgcaaaatgc agtttctttc tttgcattta
     4261 cgtgctatcg cgaaaatgct tccaccaaat aatggaaaca tcttcacaaa aataaattat
     4321 gagtttctta atttagaaac tataaacatg tcgccgctat ctggtataac cctgatgcac
     4381 ttttcttcgc aggaagttgg tattttgcga tacaggattg atctttagaa taaccccatt
     4441 tcacattaaa ctcgccttcc gcactcaagc cagaaacata agcaatacca tcttcaccta
     4501 caatgccggc catattttca gtgtctttgc tataactaat cacagcgccg aaggggatgg
     4561 gtttaccctc tttctgtttt aaggttacta atcctttatc cccgctcttc actgccaatg
     4621 tcgcaggcac aacggctcca gctgtcggta caaccaattg actagtttgt ggtaagtcgt
     4681 aattcttacc tatcgttttt tcatccagaa taacatcgtc tcttcggtat ggctgcactg
     4741 gcagtattgc atacccacga aaatcagtac tcagtcccgg ccagccatta acgttaaccc
     4801 ccgttgctcc gggtgcctct ataagggcta atgtcccatc cacattttga ccaaatgtaa
     4861 gtccatccct tgtaataagt atcccaccgc ttaaatttgc acctagctgt ttcatgtttt
     4921 cgctggagct gtaatggcta ctaaactgac catatgatcc atttttgcca acttgcacaa
     4981 tgttcatcgt gctcttcggt tgtgactgat gagcgttagt tatatttgtc gctacatccc
     5041 accatacacc accactattc tgacctgata atgaaacctg atagttatta ccctgctctc
     5101 ccgaacgact catctgtgaa gacgcataaa tttgattttc gtcacccagc gataatggca
     5161 ctgaaatacc cacgcttaat ttccgatcta ctttccattt atgcgatgtc agcaagtcgt
     5221 tttttgccca gttaatggag aatgttatgc ctctccatac cgtactataa ctcaggctgg
     5281 tagaccgcgt gtttcctgtg ccccgggaag aattatacct cgtggtattg aagttcaaat
     5341 tcccgatatt accaaggggc tgggataagg atatcgtaga gccatcgtga ctattcttat
     5401 ctctttttct gttccgttca tcctgtgcac ttactgcttc agtcagtgtg gcataacctg
     5461 atgcataacg gttcgtggtt atagacaata gactggcccc ctgaatctcc agacaaccag
     5521 tatcacttat
//
LOCUS       DAZXUL010000202         5487 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZXUL010000202 DAZXUL010000000
VERSION     DAZXUL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.203"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1632)
                     /locus_tag="ACH3L1_004110"
     CDS             complement(<1..1632)
                     /locus_tag="ACH3L1_004110"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFT4078609.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWV"
     gene            complement(1648..2543)
                     /locus_tag="ACH3L1_004111"
                     /pseudo
     CDS             complement(1648..2543)
                     /locus_tag="ACH3L1_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(2543..3148)
                     /locus_tag="ACH3L1_004112"
     CDS             complement(2543..3148)
                     /locus_tag="ACH3L1_004112"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFT4078610.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3319..>5487)
                     /locus_tag="ACH3L1_004113"
     CDS             complement(3319..>5487)
                     /locus_tag="ACH3L1_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFT4078611.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1582 a         1364 c         1141 g         1400 t
ORIGIN      
        1 tacccacggt ttaccatctt tatgactggt taacaccccg gcaatcagct ccagttgtgg
       61 gtgccacggc tgcccggtgt agatgagaat gccacccggt tcgatggcat tggctagacc
      121 tgcgagtgag ttttttacct gctcattttc gggaaaaagc tcatataggc cagagacaat
      181 cgccagcgta ggccgcggag ttaacgcgct gagttcctcc gggttaaacg catcgccctg
      241 ttcaaaacgc acccgcccag acattccccg ttgggcaatc atctcttgcc cctgtgcaac
      301 atttaactca ctgtaatcac gtaacaaaat atcgcttacg gcaggctcgt ttgccagcgc
      361 atccagtaca tagcgtccat gccctgcggc aatgtcaacc acgcggacgg ctaaaccttt
      421 ggcgtggaga tcggcaacgg cttgtttaat caatatttgc aaatgggttt tgcgctggcg
      481 aataccgcgc catccaacac tgttcaggta gattttgtcg attaaacgcc cgaatgcgtt
      541 actgccttgc ggttgatttt gatagacata atccagggaa ctgcctgaat caaagccggt
      601 actcattccg agatgcaggc ccgcagagtg cgcaccgaac aatttcatcg cctttcgcat
      661 aaagcgatac gctaaatcaa ccggcgataa tggcacgggt ccaccagaaa ggagccgcca
      721 tcgatccgct gatggtcctg tgcggtcttc atgctgataa tcaaattttt gcgatttgtt
      781 agcatataag cggcagataa agctttgcat tttttcaaat gcctgcgccc tgttctcttc
      841 acccaatgtg tcgtgataaa agcctggcag cagatgcagc tctttcagag ggctacgtaa
      901 tctctgataa aaatcaatct gcggttggcg atgcaccaca tagtcatcac ctgatatcag
      961 aagttgcgtg gggagcgtaa tcgccgccgc atcgctaaca atacgttcag acgttttata
     1021 gagatcgagc aagatattga cggcaatcgc ccgtgtgatc agcggatcat tattgaaact
     1081 cgccacccgt tgccgatcgt gggtcaaata gcgccctttc acataggaat taataaaaaa
     1141 tagaccacgc agacgatgcc ataacgccag cgcaggacgt gccagcggca catacaattt
     1201 aaccttaaag gccggagaag ccagcaccag cccacgaatt gcaggtgcat aatcatgaac
     1261 ccatgtggca acaagcactg cgccgacgct ttgcgcgatc actaccacct cttccagtcc
     1321 gacctggctg tcgctggcag caaaacggac aaattcatcg acatcctgca ctgagcgcat
     1381 aagagacggg ctataaccac gcggccccga attttttcca tgcccccggg catcccaggc
     1441 ataaaaagca gtatcaggca tcgccagttc atcaacgaga tgttgtagac gaccagaatg
     1501 ttcatgcccg cgatgaaata agacgatgac ctttttcgcc cctggctgta aagtgggcca
     1561 gtgccgataa tacaatgctg tattatcact ggtggtaaaa aaatgttccc cagggatgcg
     1621 tgaattttcc attttctcta ttttccttca gtaacagcag tagcgaataa aataaaaaaa
     1681 taccggagcg gtgaaaatta atgagtcaat cctgtcaagg agtccgccat gtcctagtaa
     1741 tagttttcca ctatctttaa caccaatatc tcgtttgatg gctgacatca ctacatcgcc
     1801 gcaaaaacca ctaataccaa ttaacaaacc cgctaataat gcctgtaatg tatttagcgg
     1861 tgtcagcaac ggtccaataa tcagtgacgc aatcatggtg gtgattacgc cccccagcag
     1921 accttccagc gtttttcccg ggctgacttt ggggaccact tttcttctgc cgcaggattt
     1981 cccccataaa tactgtgcaa tatcgtttga ctcggttaac gccaaaagaa ataacaccag
     2041 taacgccccg ccctggatat ttatggttgg caacattaat agccaggcgg catgactcaa
     2101 ggcaaaaacg gtcattatcc atccccaaaa aatggcgctc acggtatgct gaaaaccgga
     2161 ggggtcaccc acaaaaactc gccaggtggc taatatcaga aacccagtca aaggtataaa
     2221 taacagaaac agctcaaagc aattaaaacc gataagcaaa tagttaagag gaataaccca
     2281 atataaccaa cgcgaaaaat gtacggatat aagcgtacaa tattctttca atgccagaaa
     2341 acttatcagc gtaaaaaacg tcaacgtcat ccagcgtggg cctgaaatca ccagtgaaaa
     2401 acaaatgacg atgccccacc aggtacgaat acgtaatgta acttcatccc cttttctctc
     2461 aggtaaccgc catagcagaa aacggtttat cagcttgcta aaattaacaa cgcgaaaagt
     2521 gttgccagag atttttccag cattatcttt cagcgctcat aagaagcaca ctccgacaac
     2581 gattaatcgc agtacagata agcagaattg aagcaatgct ccataaaaga ttattccatt
     2641 gcatccactg tggataaatg gcaacggcca gaccccacag accaaatatt aacgcgcgat
     2701 cacttttgcc aaatggaccg gcataactgc gaacaccgtt aatcgtctgc gcgagtaaac
     2761 cgcaaaactc ggtcaatatg gtgcaaaata acatgagtat cacgagtgat gcgttacttt
     2821 ccggtaaaaa taaaaacggt aaatagagcg caatatcgga aataacatcg ccagtctcat
     2881 tcaaaatcgc ccccagacgt gtttgctgat tgcactcacg cgccaacatg ccatccagcg
     2941 cattgagcgc catacggata aaaagcacga tgggcaatag caaaaagagg atgggttgtg
     3001 ccaccaacac caacagcaat ccggtaaaaa gagaaagcgc cagtgccgta agagtgatgt
     3061 gattcgctgt aacgtggtgc ttataaagcc aaaacatcgt cggccttaac agcgactgaa
     3121 agagcggttt tatctggtag agcgtcatta tcaatccttg ataattcggt ttgcgctatg
     3181 gtgcatcaat tcccctgacg gagatatcgc aacacgccgc ttcattaaaa attcagaata
     3241 tatcaaggta ttgggggctc actgtcagga atgattatcc ccaataaatc aagtagttaa
     3301 aaattattac gctgttattc aatacaactc atcaagtttc ctcatcgctt gttcccgtat
     3361 tgcgcgcgtc ttaccgttaa gcgccagctg gcggactaaa tccagttcag ggttaacaag
     3421 ctctaacgcc agcaaaggat cacgtttcag cctcgccttc aatacatcaa ctggaaagcg
     3481 gggattattc atcgccacaa tccaccactc gggatcaatt tctgccgcca gtgccgcaga
     3541 gggggtgtgg cggttgctaa gtgcgccgaa cttgaaaaat gtgagattgc ttttcgccag
     3601 tgtactccac catatacgat ccggttgttt tgccagtaca tccagttgtg ctaacgcatc
     3661 ggggtcgagt gcataataaa cacgtaatgc ttcatcaacc tgcgcacggg agctattttc
     3721 ctgcctcaga aaaaacttcg cccaaatatc attgcgtggc gtgttttgct gtaattcctt
     3781 gagaacaaca gcgggtaaat tatcccaact aactaatgca ctctctaaaa agtcccagtc
     3841 ttccaaacgt atcagggcgg cggcatttaa tagcatctgg tcatcaactt cgccgtggct
     3901 aatcagctca gacaaaatca tatcagcgct ggtttcatat gcatctgaat ctgctttcgg
     3961 attatttttc cgatgcgata aagcacgttg ccaaattttt tgtttcatcg acgtgctcag
     4021 atactgattt ttcgccagat tactccaccc ctcaggatcg ttgaaacgtg tcaataaccg
     4081 ttgtaaagca gcgtcagagt gaattacctg catatcccga cagcaaaagt aggcagggga
     4141 ttttttctcc atccttttaa caaaattaaa ctgtaagtta tctggcaatg ctaaaaataa
     4201 cacacgtatt agatcatcgt cattctcgaa tttttggtat aaataggctg caactttgtc
     4261 actctcttgt ggcgtcatcc gcaataattg accatggtgt ttcatctctg ccagcttgaa
     4321 cgctaagctt gtgacaacat tgtttgatga atcgttcgcc aactgcattg cctgattgta
     4381 agtgagttgt cctccagcac cgacctgacg aatagaggaa tcatcactgc ggatatagcc
     4441 atcaataatg gattcgggca ataaccacgc atctttataa agctcctgaa tgatcatatc
     4501 ccaatctctt ttcgccagag tcatgacgat atccagaggg agattttcac gtagcgcaag
     4561 attcaacgga ccgacaccat caggtagacg actcattcgc gtcattattt cgacagagag
     4621 cggttcacgg tcgagcaacc ccagaacaac aggctggtaa cccaattgct gatcggtcaa
     4681 ctccagaatc tcattgtcag gcatatatct cgccatactt tcaatcgttt gttcatcatt
     4741 gctattaata agttgtttgc gtaggttagc tgaaattcct ttaagccttg ggagagttcg
     4801 cattatggca agatcaaatg tcgcaatatc tcgtccttca aatgcagcaa cgttgccttc
     4861 atccagcatt tgcaattcct gcactaactc aggagaaggc ttagggcgaa acagcgcttc
     4921 ttttttcatc tgattacaga cattccctgc catgcgaata ttgcttcgct taaaaatttc
     4981 tttcagagca tcttcatcat ttattagacc ccaataatat gcatcaggat ttattatcat
     5041 cagcgctttt ttgacatttt cagtgtcatt tttgtcactc gcacggacca tgaattcact
     5101 atttgtcgaa gacgcaacaa caggtgcaat tatgtcattg ataaataccc cacgcgcatt
     5161 aatactcccc ttaatcgtag acggattatc gtaatatgag tcgataaata ccagtccact
     5221 tgcatttatc gaaccggtaa ttaacagtgg tgaatccgta actatattcg ttgccgtcac
     5281 gttaccgaga ataacccatg gattagaaag agagaaagtg gaaatattac ctttgactgt
     5341 caaattgcca agaacaatca atttttgatt actctctata ttaccttcta ccactaaatc
     5401 gccacgatgc actttccagt ccgatgcaga taaaccgaag gggacaagat tcacgctact
     5461 gttgatagct tgttcaactt gttgttt
//
LOCUS       DAZXUL010000203         5473 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZXUL010000203 DAZXUL010000000
VERSION     DAZXUL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.204"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            511..1500
                     /gene="araF"
                     /locus_tag="ACH3L1_004114"
                     /pseudo
     CDS             511..1500
                     /gene="araF"
                     /locus_tag="ACH3L1_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            1570..3084
                     /gene="araG"
                     /locus_tag="ACH3L1_004115"
     CDS             1570..3084
                     /gene="araG"
                     /locus_tag="ACH3L1_004115"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFT4078612.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3099..4085
                     /gene="araH"
                     /locus_tag="ACH3L1_004116"
     CDS             3099..4085
                     /gene="araH"
                     /locus_tag="ACH3L1_004116"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFT4078613.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4252..5052
                     /gene="otsB"
                     /locus_tag="ACH3L1_004117"
     CDS             4252..5052
                     /gene="otsB"
                     /locus_tag="ACH3L1_004117"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFT4078614.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            5027..5473
                     /locus_tag="ACH3L1_004118"
                     /pseudo
     CDS             5027..5473
                     /locus_tag="ACH3L1_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
BASE COUNT         1350 a         1266 c         1466 g         1391 t
ORIGIN      
        1 atgaagtgat gattttataa aacgtttttt catttttgcg agatggctct cattatacgt
       61 gttctgttaa ttaatgagca cagtgataat taatggcgaa atgaatgacg cgcattttcc
      121 acatctttga gttgtggtta ttacaccatt tcaaaaactc aacgccaggt aatgcggcct
      181 attgactggt taaaaagaag acatcccgca tgggtaccga agacaacaag gatttccagg
      241 ctaatcttat ggattaatct gctgtgcatt caacaatttg tctgacaaat tggcttttcc
      301 ttatgtcttt tcccgctaaa tttatgcacg ttctcactgt aattctgcga tgtgatattg
      361 ctctcctatg gagaattaat ttctcgctaa aactatgtca acacggtcac ttatctttta
      421 gataaaaggt aatgctttgt tttccgatta atttaacgaa tgtcattcgt ttttgcccta
      481 cacaaaacga cactaaagct ggagagaacc atgcacaaat ttactaaagc cctggcagcc
      541 attggtctgg cagccgttat gtcacaatcc gctatggcgg agaacctgaa gctcggtttt
      601 ctggtgaagc aaccggaaga gccgtggttc cagaccgaat ggaagtttgc cgataaagcc
      661 gggaaggatt tagggtttga ggttattaag attgccgtgc cggatggcga aaaaacattg
      721 aacgcgatcg acagcctggc agccagtggc gcaaaaggtt tcgttatttg tactccggac
      781 ccgaaactcg gctctgccat cgtcgcgaaa gcgcgtggct acgatatgaa agtcattgca
      841 gtggatgacc agtttgttaa cgccaaaggt aagccaatgg ataccgttcc tctggtgatg
      901 atggcggcga ctaaaattgg cgaacgtcag ggctaggaac tgtataaaga gatgcagaaa
      961 cgtggctggg atgtcaaaga aagcgcagtg atggcgatta ccgccaacga actggatacc
     1021 gcccgccgcc gtacaacggg ttctatggac gcactgaaag ctgcaggatt cccggaaaaa
     1081 caaatttatc aggtacctac caaatctaac gacatcccgg gggcatttga cgctgccaac
     1141 tcaatgctgg ttcaacatcc ggaagttaaa cattggctga tcgtcggtat gaacgacagc
     1201 accgtacttg gcggcgtacg tgcaaccgaa ggtcagggct ttaaagcagc cgatatcatc
     1261 ggtattggta ttaacggtgt ggatgcagta agcgaactgt ctaaagcaca ggcaaccggc
     1321 ttctacggtt ccctgctgcc aagcccggac gtacatggct ataaatccag cgaaatgctt
     1381 tacaactggg tagcaaaaga cgttgaaacg ccaaaattta ccgaagttac agacgtggta
     1441 ctgatcacgc gtgacaactt taaagaagaa ctggagaaaa aaggtttagg cggtaagtaa
     1501 tttgccggaa aaattcccct ctgcatgatg cagagggggt gtgaacgacc agtgattcac
     1561 ggagacgtta tgcaacagtc taccccgtat ctctcatttc gcggcatcgg taaaacgttt
     1621 cccggcgtta aggcgctgac ggatattagt tttgactgct atgccggtca ggttcatgcg
     1681 ttgatgggtg aaaatggcgc aggaaaatca actctcttaa aaatcctcag cggcaactat
     1741 gcgccaacca cgggttctgt agtgattaat gggcaggaaa tgtccttttc cgacacgacc
     1801 gcagcactta acgcgggtgt ggcgattatt taccaggaac tgcatctcgt gccggaaatg
     1861 accgtcgcgg aaaacatcta tctcggccag ctgccgcata aaggcggcat tgtgaatcgc
     1921 tcattgctga attatgaggc gggtttacaa cttaaacatc ttggtatgga tattgacccg
     1981 gacacgccgc tgaaatatct cgccattggt cagtggcaga tggttgaaat cgccaaagcg
     2041 ctggcgcgta acgctaaaat tatcgccttt gatgagccaa ccagctccct ttctgcccgc
     2101 gaaatcggca atcttttccg cgttattcgt gaactgcgaa aagaggggcg ggtgatctta
     2161 tacgtttctc accgtatgga agaaatattt gccctcagcg atgccatcac tgtctttaaa
     2221 gatggacgtt atgtcaaaac ctttaccgat atgcagcagg ttgaccacga cgcgctggtg
     2281 caggcgatgg tcgggcgcga cattggcgat atctacggct ggcaacctcg tagttatggc
     2341 gaggagcgcc tgcgtcttga tgctgtgaaa gcactaggcg tgcgtacgcc aataagtctg
     2401 gcggttcgca gtggtgaaat tgttgggctg tttggtctgg taggggcggg gcgtagcgaa
     2461 ttaatgaaag gcttgtttgg cgggacgcaa atcaccgccg gtcaggttta tatcgaccaa
     2521 cagccgatcg atattcgtaa accgagccac gccattgccg caggcatgat gctctgcccg
     2581 gaagctcgca aagcggaagg cattattccc gtgcactccg ttcgcgacaa tatcaacatc
     2641 agtgccagac gcaaacatgt gctcggcggt tgtgtaatca acaacagttg ggaagaaaac
     2701 aatgccgatc agcacattcg ttcgctcaac atcaaaacgc cgggcgcgga gcaactgatc
     2761 atgaatctct caggcggaaa tcagcaaaaa gccattctgg gccgctggtt atcggaagag
     2821 atgaaggtca ttttgctgga tgaacctacg cgcggcattg atgttggcgc taagcacgaa
     2881 atatataacg taatttatgc gctggcggcg cagggcgtgg cggtgctgtt tgcctccagc
     2941 gacttacctg aagtcctcgg cgttgccgac cggattgtgg tgatgcggga aggcgaaatc
     3001 gccggtgaat tgttacacga gcaggcagat gagcgtcagg cactgagcct tgcgatgcct
     3061 aaagtcagcc aggcagttgc ctgagtaagg agagtatgat gtcttctgtt tctacatcgg
     3121 ggtctggcgc acctaagtcg tcattcagct tcgggcgtat ctgggatcag tacggcatgc
     3181 tggtggtgtt tgcggtgctc tttatcgcct gtgccatttt tgtcccaaat tttgccacct
     3241 tcattaatat gaaagggttg ggcctggcaa tttccatgtc ggggatggtg gcgtgtggca
     3301 tgttgttctg ccttgcttcc ggtgactttg acctttctgt cgcctccgta attgcctgtg
     3361 cgggtgtcac cacggcggtg gttatcaacc tgactgaaag cctgtggatt ggcgtggcag
     3421 cgggtttgtt gctgggcgtt ctctgtggac tggtcaatgg ctttgttatc gccaaactga
     3481 aaataaatgc tctgatcacg acactggcaa cgatgcagat tgttcgaggt ctggcgtaca
     3541 tcatttcaga cggtaaagcg gtcggtatcg aagatgaaag cttctttgcc cttggttacg
     3601 ctaactggtt cggtctgcct gcgccaatct ggctcaccgt cgcgtgtctg attatctttg
     3661 gtttgctgct gaataaaacc acctttggtc gtaataccct ggcgattggc gggaacgaag
     3721 aggctgcgcg tctggcgggt gtaccggttg ttcgcaccaa aattattatc tttgttctct
     3781 ctggcctggt atctgcgata gccggaatta ttctggcttc acgtatgact agtgggcagc
     3841 caatgacgtc gattggttat gagcttattg ttatctccgc ctgcgtttta ggtggcgttt
     3901 ctctgaaagg tggcatcgga aaaatctcat atgtggtggc gggtatctta attttaggca
     3961 ccgtggaaaa cgccatgaac ctgcttaata tttctccttt cgcgcagtac gtggttcgcg
     4021 gcttaatcct gctggcagcg gtgatcttcg accgttacaa gcaaaaagcg aagcgcactg
     4081 tctgatgctt ttttccacaa caacttaacg ttttttccca ccacagccag ccgccacaac
     4141 ggttggctgt tcttcgttgc aaatggcgac ctccgtcaca ctgtctatac ttacatgtct
     4201 gtaaagcgcg gtctgcgcaa cacaataaga aaagagaagg aggagagccg ggtgacagaa
     4261 ccgttaaccg aaacccctga actatccgcg aaatatgcct ggttttttga tcttgatgga
     4321 acgctggcgg aaatcaaacc gcatcccgat caggtcgtcg tgcctgacaa tattctgcaa
     4381 ggactacagc tactggcaac cgcaagtgat ggtgcattgg cattgatatc agggcgctca
     4441 atggtggagc ttgacgcact ggcaaaacct tatcgcttcc cgttagcggg cgtgcatggg
     4501 gcggagcgcc gtgacatcaa tggtaaaaca catatcgttc atctgccgga tgcgattgcg
     4561 cgtgatatta gcgtgcaact gcatacggtc atcgctcagt atcccggcgc ggagctggag
     4621 gcgaaaggga tggcttttgc gctgcattat cgtcaggctc cgcagcatga agacgcatta
     4681 atgacattag cgcaacgtat tactcagatc tggccacaaa tggcgttaca gcagggaaag
     4741 tgtgttgtcg agatcaaacc gagaggtacc agtaaaggtg aagcgattgc agcctttatg
     4801 caggaagctc cttttatcgg gcgaacgccc gtatttctgg gcgatgattt aaccgatgaa
     4861 tctggcttcg cagtcgttaa ccgactgggc ggaatgtcag taaaaattgg cacaggtgca
     4921 actcaggcat catggcgact ggcgggtgtg ccggatgtct ggagctggct tgaaatgata
     4981 accgccgcat tacaacaaaa aagagaaaat aacaggagtg atgactatga gtcgtttagt
     5041 cgtagtatct aaccggattg caccaccaga cgagcacgcc gccagtgccg gtggccttgc
     5101 cgttggcata ctgggggcac tgaaagccgc aggcggactg tggtttggct ggagtggtga
     5161 aacagggaat gaggatcagc cgctaaaaaa ggtaacatta cgtgggcctc ttttaacctc
     5221 agcgaacagg accttgacga atactacaac caattctcca atgccgttct ctggcccgct
     5281 tttcattatc ggctcgatct ggtgcaattt cagcgtcctg cctgggacgg ctatctacgc
     5341 gtaaatgcgt tgctggcaga taaattactg ccgctgttgc aagacgatga cattatctgg
     5401 atccacgatt atcacctgtt gccctttgcg catgaattac gcaaacgtgg ggtgaataat
     5461 cgcattggtt ttt
//
LOCUS       DAZXUL010000204         5401 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZXUL010000204 DAZXUL010000000
VERSION     DAZXUL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.205"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..497
                     /locus_tag="ACH3L1_004119"
     CDS             <1..497
                     /locus_tag="ACH3L1_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HFT4078615.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACH3L1_004120"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACH3L1_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACH3L1_004121"
     CDS             2500..3114
                     /locus_tag="ACH3L1_004121"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFT4078616.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACH3L1_004122"
     CDS             3114..3443
                     /locus_tag="ACH3L1_004122"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFT4078617.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACH3L1_004123"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACH3L1_004123"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFT4078618.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="ACH3L1_004124"
     CDS             4529..4840
                     /locus_tag="ACH3L1_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFT4078619.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..5373
                     /locus_tag="ACH3L1_004125"
                     /pseudo
     CDS             4824..5373
                     /locus_tag="ACH3L1_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1178 a         1344 c         1426 g         1453 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggcaag
     5401 t
//
LOCUS       DAZXUL010000205         5378 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZXUL010000205 DAZXUL010000000
VERSION     DAZXUL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.206"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(75..557)
                     /locus_tag="ACH3L1_004126"
     CDS             complement(75..557)
                     /locus_tag="ACH3L1_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078620.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="ACH3L1_004127"
     CDS             complement(625..2574)
                     /locus_tag="ACH3L1_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFT4078621.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="ACH3L1_004128"
     CDS             complement(2659..2994)
                     /locus_tag="ACH3L1_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFT4078622.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="ACH3L1_004129"
     CDS             complement(2994..3350)
                     /locus_tag="ACH3L1_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFT4078623.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="ACH3L1_004130"
     CDS             complement(3350..4846)
                     /locus_tag="ACH3L1_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFT4078624.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="ACH3L1_004131"
     CDS             complement(4843..5004)
                     /locus_tag="ACH3L1_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFT4078625.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5378)
                     /locus_tag="ACH3L1_004132"
     CDS             complement(5014..>5378)
                     /locus_tag="ACH3L1_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078626.1"
                     /translation="PLLPVHLNERDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGE
                     IVYVGGTLLDLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPG
                     TGPDGDIEHHLEMRFPQN"
BASE COUNT         1282 a         1566 c         1159 g         1371 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg tatttttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5341 ttcatcacgc tcattgaggt gtactggcaa tagcggac
//
LOCUS       DAZXUL010000206         5340 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZXUL010000206 DAZXUL010000000
VERSION     DAZXUL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.207"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACH3L1_004133"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACH3L1_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFT4078627.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACH3L1_004134"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACH3L1_004134"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFT4078628.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACH3L1_004135"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACH3L1_004135"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFT4078629.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACH3L1_004136"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACH3L1_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFT4078630.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1458 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
//
LOCUS       DAZXUL010000207         5261 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZXUL010000207 DAZXUL010000000
VERSION     DAZXUL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.208"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACH3L1_004137"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACH3L1_004137"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFT4078631.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACH3L1_004138"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACH3L1_004138"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFT4078632.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3324)
                     /locus_tag="ACH3L1_004139"
                     /pseudo
     CDS             complement(2698..3324)
                     /locus_tag="ACH3L1_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3454..5214
                     /gene="ycaO"
                     /locus_tag="ACH3L1_004140"
     CDS             3454..5214
                     /gene="ycaO"
                     /locus_tag="ACH3L1_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFT4078633.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1343 a         1356 c         1258 g         1304 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatcac attaccaggc
     3061 ggtataacaa cgccagagtg ataaagacga taagtaccgg caccatcggc acatcatcat
     3121 aaaacgccac atctcccgcc gctgaaccca gcgtgagaat gattaacact tcaaacagcg
     3181 acatctgtcg cacaccgcgt cttccggtca ttttgaggaa caaaaaacca gtacaaaggt
     3241 ataaagacta cgtagtgcaa cttcgccaag gaaatcaaaa ggcactttat caaatgccat
     3301 acggtggaga tcgaacgcct tcatttttat gctcttaacg tggtttatcc gctaaaagca
     3361 tagtcaacag ttcatcggca tggcggcggt gatagcgcct ttgagataaa acccgtatta
     3421 ttgcgcgctt tccgtacgac taaagtgatt ttcatgacgc aaacatttat ccccggcaaa
     3481 gatgccgctc tggaagattc catcgctcgc ttccagcaaa aactttcaga cctcggcttt
     3541 cagattgaag aggcctcctg gctgaatccc gtgcctaacg tctggtctgt acatattcgc
     3601 gacaaagagt gcgcactgtg ttttaccaac ggtaaaggcg caaccaagaa agcggcgctg
     3661 gcttctgcac tcggtgaata tttcgagcgt ctctcaacca actacttttt tgctgatttc
     3721 tggctgggcg aaaccatcgc caacggtcca tttgtgcatt atcccaacga aaaatggttc
     3781 ccactgagcg aaaatgacga tgtgccagaa gggctgctcg atgaccgtct gcgcgcattt
     3841 tacgatccgg agaatgaatt gaccggcagt atgctgattg acctacaatc cggtaacgaa
     3901 gatcgtggta tttgcggtct gccgtttacg cgccagtccg acaatcagac cgtttatatt
     3961 ccgatgaata tcattggtaa cctgtacgtt tctaacggta tgtccgctgg caatacccgc
     4021 aacgaagcac gcgttcaggg gttgtccgaa gttttcgaac gctacgtgaa aaaccgcatt
     4081 attgctgaaa gcatcagctt gccggaaatc ccggcagacg tgctggcgcg ttacccagcg
     4141 gtggttgaag cgatcgaaac actggaaaca gaaggtttcc cgatcttcgc atatgatggt
     4201 tcccttggcg gccagtatcc ggtgatttgc gtggtactgt ttaatcctgc aaacggcacc
     4261 tgctttgcct ctttcggtgc gcatcctgat tttggcgtag cactggaacg taccgtgacc
     4321 gagctgctgc aaggtcgtgg cctgaaagat ttggatgtgt ttactccgcc aaccttcgat
     4381 gatgaagaag tcgctgaaca taccaacctc gaaacgcact ttatcgattc cagcggttta
     4441 atctcctggg acctgttcaa gcaggatgcc gattatccgt ttgtggactg gaatttctcc
     4501 ggcaccacgg aagaagagtt tgctacgctg atggctatct tcaacaaaga agataaagaa
     4561 gtttatattg ccgattacga gcatctgggc gtttatgctt gccgtattat cgtgcctggc
     4621 atgtccgata tttatccggc tgaagatctg tggctcgcga ataacagtat gggcagccat
     4681 ttacgtgaaa cgattctttc gctaccaggc agcgagtggg aaaaagaaga ttacctgaac
     4741 ctcatcgagc aactggatga agaaggtttt gatgacttta cccgcgtgcg tgagctgttg
     4801 ggtctggcga ccgggtcgga taacggttgg tacaccctgc gtatcggtga attaaaagcc
     4861 atgctggcgc tggctggtgg cgatctggaa caggctctgg tctggaccga atggacgatg
     4921 gagtttaact catcagtatt tagtccggaa cgcgccaact attatcgctg cctgcaaacg
     4981 ttgttattac tggcacagga agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt
     5041 cgcatgtacg gcgcagatgc cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg
     5101 ttttacggcc tgcaaccggt agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg
     5161 ttgaaggcct acgaaaagct gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa
     5221 atagcctacg caatgtaggc ttaatgatta gtctgagtta t
//
LOCUS       DAZXUL010000208         5200 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZXUL010000208 DAZXUL010000000
VERSION     DAZXUL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.209"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(56..817)
                     /gene="kduD"
                     /locus_tag="ACH3L1_004141"
     CDS             complement(56..817)
                     /gene="kduD"
                     /locus_tag="ACH3L1_004141"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFT4078634.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(847..1683)
                     /gene="kduI"
                     /locus_tag="ACH3L1_004142"
     CDS             complement(847..1683)
                     /gene="kduI"
                     /locus_tag="ACH3L1_004142"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFT4078635.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(1970..3151)
                     /locus_tag="ACH3L1_004143"
     CDS             complement(1970..3151)
                     /locus_tag="ACH3L1_004143"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFT4078636.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3406..4635
                     /locus_tag="ACH3L1_004144"
                     /pseudo
     CDS             3406..4635
                     /locus_tag="ACH3L1_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5062..>5200
                     /locus_tag="ACH3L1_004145"
     CDS             5062..>5200
                     /locus_tag="ACH3L1_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4078637.1"
                     /translation="MQLYTDREVLNMGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCA
                     NS"
BASE COUNT         1402 a         1290 c         1107 g         1401 t
ORIGIN      
        1 tagagatata aaaaaccctg ccatgcggca gggtcataaa agtaagagga atgaattaac
       61 gcgccagcca accgccatcc acagcaatgg tataaccatt tacataatct gaagcgctgg
      121 aggcaagaaa cactaccggc cccatcaggt cactcggcag tccccaacgg ccagctggaa
      181 tgcgatcgag aatttccgcg ctacgttgtt catctgcccg cagttgttgg gtattgttgg
      241 tcgccatgta acccggagct atcgcattaa cattaatgtt gtgtttagcc cattcgtttg
      301 ccatcaatcg cgtcacaccc atcacgccgc tttttgatgc ggtataagaa ggcacacgga
      361 tcccgccctg gaaggagagc attgacgcga tattgataat cttgccgcca ttgccttgcg
      421 cgataaagtg tttcgccgct gcctgagaca tgaagaatac gctcttgata ttcaggttca
      481 tgacatcgtc ccagtctttt tcgctgaact cgagagcgtc ttcgcggcga atcaatccgg
      541 cgttattcac caggatatca atatgaccaa attcagctac cgcgcgatcc agcagtccag
      601 gaatgccatc aatctttcgc agatcggcgg tcaggcttaa aaaacgacgc cccagcgccg
      661 tgacctgctc gatggtttca gtcggttcaa cgatgttaat gccaacaatg tcacagcccg
      721 cttgcgccag ccccaacgcc atcccctggc ccagcccagt atcacaacca gtgacgaccg
      781 caactttacc ttggagagaa aatgcactta aaatcataac aacaccttat tcgtttatgc
      841 ccacaactag cgcaaatctt taacgcccac atggtccata tcattaaaga cctggttttc
      901 accgaccatg ccccagataa aggtataagc tttggttccg acgccggaat ggatcgacca
      961 gctcggggag atcaccgcct gctcgttatg catcacaatg tgacgcgttt cttgcggctg
     1021 ccccatcatg tggaaaacgc aggcgtcatc atccatattg aaatagaaat aaacttccat
     1081 ccggcgctcg tgggtgtgac acggcatggt gttccacaag ttacctggag ccagctccgt
     1141 cagccccata ctcaattgac aggtttccag tacatccggg acaaaatatt tgttaatcgt
     1201 gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga gatacttcgt ccggtgtgac
     1261 ttttttggtg ggatacgtcg tatgcgcggg tgcgcaattg taataaaact tcgccggagt
     1321 ggcggtatca atactggcaa agacaacttc ttttgcacct ttaccaacat acagggcgtc
     1381 gcggtgaccg atttcatagc attgaccatc gacagtaatc gtaccggcac cgccaatatt
     1441 gataacacct aactcgcgac gttcaaggaa atagcttacg ccgagttgtt taccaacttc
     1501 cccgccaacg gaaaccgttt tagttatcgg cataatgccg ccaacaataa ttcggtcaat
     1561 gtggctgtaa accatggtgt actcatcggc gacaaatacc ttttcaacca aaaattcatt
     1621 gcgcagccct tgggtatcca gcgtttttgc gtgcgcaccg tggatgctct gtcttacgtc
     1681 cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacgatagtg aaacactgtt
     1741 tcgttttctc tataaaggga tgatatgccc atgggctagg gttttcaatt aaaaataaaa
     1801 caatgtttta tttttaattt aaggattaaa aaaagtgcag tgtcgatcac gaatatgtca
     1861 tgagaatgga aaaatgaggg agcccagaaa atgtatgtcg acttgccagc catcattatt
     1921 caacagatta atgacggcag atatttatcg gtaaggagga aatgcagagc tactcgtcac
     1981 gttcaatggt caatgccacg ccctggcccc cgccgataca aagcgttgcc agccctttgc
     2041 gggcattacg tttcaccatt tcatgaacca gagaaaccag gattcggcaa ccggaagcgc
     2101 ctatcgggtg accgagtgcg atcgcgccac cattgacatt gacccgacgc tcatcccact
     2161 caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc tcgataagat
     2221 cgacatcagc caactgccag cctacacgct ccaggcaacg gcgggtcgca tacaccggcg
     2281 caattcccat caatgccgga tctacaccaa cgctggcaaa tgcgcggatg cgggccagca
     2341 cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattaccgca gctgcgccat
     2401 cgtttatgga tgatgcattc cccgcggtca cagaaccgag actatcaaat gaaggattta
     2461 aacgggctaa accttctgca ctggcgtcag tgcgtggctg ttcatcggta tcaacaacca
     2521 acgtctgacc gttactttgg atcattaccg ggacgatctc atctttaaat cgtccggcgt
     2581 caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc aactgacggc
     2641 tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa tcattgaagg
     2701 catcccacaa cccatcatgc accagactgt caaccaactg gctattgcca agctgttcac
     2761 cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg ccacctgcga
     2821 tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt gctttaagac
     2881 cggaaccgca aacgtcatta atcgtgattg cagaaacgct gttaggaaga ccacctttaa
     2941 tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga ccaagaatta
     3001 cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttc acgaccagac
     3061 taccaagttc cacggcggaa tgacccgcta acgcaccacg aaagcagccg ataggtgtcc
     3121 gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta acagtgcaat
     3181 agtaaatgat tgttaattac agttatctta attgtttaaa aaaagtgatt tttatcacaa
     3241 aggaaatatg cctgagcagt agtcagagtc ataactggca cgtaaggttt gcaaccacta
     3301 acccaccaat agatgggtag atagggcgtt aatctcccat acttaacttg gtttatggta
     3361 aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc taatatttgg
     3421 tctaaagaag aaactctgtg aagtttcgcg ctctatggca cagccgttga tgcaggcacg
     3481 ctattccttc ctattcagtt aggttcggca ggggctgtgg tcctgtttat tactgctctg
     3541 gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat cctctcatca
     3601 aaaacatcag caggtgaagg gataacgggc gcggtaacac actactatgg caagaagatt
     3661 ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt gatatatgca
     3721 gtggcaatta ccaactcact tacggaacag ctggcaaagc atatggttat tgatcttcgc
     3781 atccgtatgt tggtgagtct gggtgttgta ttaattctga atctcatttt tctgatggga
     3841 cgtcatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc ctatttctta
     3901 tttctttcca tttaccttgt cggtagttgg caacctgatc tattaacaac ccaggtagag
     3961 ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt tttcgccttt
     4021 agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata tggcgaacac
     4081 gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg cataagtgta
     4141 ctgttctttg tctttagctg cctgctttct attccatctt cgtatattga agcggctaaa
     4201 gaagaagggg tcaccatttt atcggcgctt tctatgctgc cgaacgcccc agcatggttg
     4261 tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct gggtacgtac
     4321 tttggcgtta ttgaaggtgc cacagaggtc gtcaaaacaa cactacagca ggttggtgta
     4381 aagaaaagtc gtgcatttaa ccacgcacta tcaattatgt tggtatcgct gattaccttc
     4441 attgtttgtt gcattaaccc gaacgcgatt tcgatgattt acgcgatcag cggcccgctc
     4501 attgccatga tacttttcat catgcctacg ctgtcaacgt atctcatccc ggcgcttaaa
     4561 ccctggcgtt ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg cgtatcggta
     4621 atgttcttta gctaatatcc gttgtggatg gttcaaaatt gctacaattt tgaaccattc
     4681 catttttcat aatattcatt tgttattatt ccgccctatc acttctctcg tcatatccta
     4741 gaattatcag ttagttaaac acacaaagag atctgaacat cttaaataag actatttaag
     4801 atgcataact tagattcgca agatatcttc tggtcattca aaaacaattt gcgatataga
     4861 tattctcata ccatgagata gttatctgga ccacgttcga ccatgcaaga ttaaatcagt
     4921 gaaacataat catatttaat aacgagaaat gcatttttaa atatcttaat cagaattata
     4981 gcaaatacag attaatcgca atttttccca gcaaaattac aaaatcaagg tgtattgaca
     5041 tcatcaatca ccaatgaaat tatgcaatta tatacggata gggaggttct taacatgggg
     5101 ctttgtagtc gttataaaag tcttacatgc aatagttgct caatgcattg ccaaataatg
     5161 ccagaagagt caccgcgttt gcagtactgc gcaaactcgt
//
LOCUS       DAZXUL010000209         5183 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZXUL010000209 DAZXUL010000000
VERSION     DAZXUL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.210"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..746
                     /locus_tag="ACH3L1_004146"
     CDS             <1..746
                     /locus_tag="ACH3L1_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4078638.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="ACH3L1_004147"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="ACH3L1_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFT4078639.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3810
                     /gene="safA"
                     /locus_tag="ACH3L1_004148"
     CDS             3607..3810
                     /gene="safA"
                     /locus_tag="ACH3L1_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFT4078640.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="ACH3L1_004149"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="ACH3L1_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFT4078641.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..>5183
                     /locus_tag="ACH3L1_004150"
     CDS             5041..>5183
                     /locus_tag="ACH3L1_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFT4078642.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT         1481 a         1104 c         1156 g         1442 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aaccacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctggaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctccgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 gatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt tac
//
LOCUS       DAZXUL010000210         5164 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZXUL010000210 DAZXUL010000000
VERSION     DAZXUL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.211"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..220
                     /locus_tag="ACH3L1_004151"
     CDS             <1..220
                     /locus_tag="ACH3L1_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4078643.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="ACH3L1_004152"
     CDS             complement(392..721)
                     /locus_tag="ACH3L1_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFT4078644.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="ACH3L1_004153"
     CDS             complement(726..911)
                     /locus_tag="ACH3L1_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFT4078645.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="ACH3L1_004154"
     CDS             complement(908..3547)
                     /locus_tag="ACH3L1_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFT4078646.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="ACH3L1_004155"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="ACH3L1_004155"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFT4078647.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5164
                     /gene="hslJ"
                     /locus_tag="ACH3L1_004156"
     CDS             4855..>5164
                     /gene="hslJ"
                     /locus_tag="ACH3L1_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFT4078648.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1308 a         1323 c         1203 g         1330 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5161 aaca
//
LOCUS       DAZXUL010000211         5151 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZXUL010000211 DAZXUL010000000
VERSION     DAZXUL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.212"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            190..447
                     /locus_tag="ACH3L1_004157"
                     /pseudo
     CDS             190..447
                     /locus_tag="ACH3L1_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="ACH3L1_004158"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="ACH3L1_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFT4078649.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="ACH3L1_004159"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="ACH3L1_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFT4078650.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="ACH3L1_004160"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="ACH3L1_004160"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFT4078651.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="ACH3L1_004161"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="ACH3L1_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3215..3431
                     /locus_tag="ACH3L1_004162"
                     /pseudo
     CDS             3215..3431
                     /locus_tag="ACH3L1_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3605..4420)
                     /locus_tag="ACH3L1_004163"
     CDS             complement(3605..4420)
                     /locus_tag="ACH3L1_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4078652.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5151
                     /locus_tag="ACH3L1_004164"
     CDS             4517..>5151
                     /locus_tag="ACH3L1_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFT4078653.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1374 a         1175 c         1226 g         1376 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DAZXUL010000212         5121 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZXUL010000212 DAZXUL010000000
VERSION     DAZXUL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.213"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1739
                     /locus_tag="ACH3L1_004165"
     CDS             <1..1739
                     /locus_tag="ACH3L1_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078654.1"
                     /translation="EMHQKFWDQRYNEISRIIDAEGVFSQEQRRIIYARYEHFYYMMN
                     SYPVHSTLKPEFLRSYCLRTFGVIFLVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCL
                     DHADTESDEAAVKRYLREYLAELGFNKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQ
                     QYIMAIKNEYKKDYREKKLDKDELEKVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLA
                     YYLRVLVREGLWIHGLYGYAAQYLYDFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYS
                     VSLDDKSQEYISRLKDLVFNINDKNGCDDAYLKKIISYFGQLQNEAVHVTSCYETLAV
                     YICLIRKNKINDVLQHYDDMERKGLFGELPSGYVRGALSLLRTALEVKVNRKNIKYGS
                     LFYWLDHVKAYQDAFIEKIPLIDPVYKEGEIQYDANNFTLMRVIKMYNCMLEKISTKP
                     YIAPPYITGLLDDVEKVLDKINILIDKEYVYDGKTLAEVIMENKVLSSRERKETMIGL
                     FTGSKKYTLLQCVEKLGVLVHYVKSPVDEIKNVMMLYGDKAENRNRRRMIYDALTIIC
                     EDDIRNNPPELS"
     gene            3114..3677
                     /locus_tag="ACH3L1_004166"
     CDS             3114..3677
                     /locus_tag="ACH3L1_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFT4078655.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            3784..4130
                     /locus_tag="ACH3L1_004167"
                     /pseudo
     CDS             3784..4130
                     /locus_tag="ACH3L1_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            4194..4756
                     /locus_tag="ACH3L1_004168"
                     /pseudo
     CDS             4194..4756
                     /locus_tag="ACH3L1_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            4784..>5121
                     /locus_tag="ACH3L1_004169"
     CDS             4784..>5121
                     /locus_tag="ACH3L1_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFT4078656.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
BASE COUNT         1489 a          889 c         1114 g         1629 t
ORIGIN      
        1 ttgaaatgca tcaaaaattc tgggatcaac gttataatga gatcagtcgt attattgatg
       61 ctgaaggggt gttcagtcag gagcaacggc ggatcatata tgcccgatat gaacattttt
      121 attacatgat gaattcttat ccagtgcatt ccacactaaa acctgaattc cttcgcagct
      181 attgtctgcg cacttttggg gttatttttc ttgttgtaga tatgtataat acatacagac
      241 cagagaatga ctctgctttt tattatcata tttataattt tttacagaaa tcgtattgtc
      301 cttgtcttga tcacgctgat acagaaagcg atgaagctgc tgttaaacgt tatcttcggg
      361 aatatctggc tgaactggga ttcaacaagg aagattttca tgaaaatgga aagctgtatg
      421 ctctggggaa atatacagga actatacgga aggataacgg gaaaagcaag agcctgatgc
      481 aacaatatat catggctatc aaaaatgaat ataaaaagga ttaccgggag aagaagctgg
      541 ataaggatga actggagaaa gtgctcagga atattgataa attctataat gcattttatt
      601 cattaagtgt cttacttgat atacaacgaa agacgaagat acttcaaagc cttgcatatt
      661 atctcagagt gttggtgaga gaaggactct ggattcatgg tttgtatggt tacgctgcgc
      721 aatatcttta tgactttacc agctttgata caacgcctta tgcaaaaaaa ttactggaaa
      781 tgttttataa gtttcaaaac agtgcagaag gaacattgtc tcgttatagt gtttcacttg
      841 atgataaatc tcaggaatat atttcccgtc tgaaagatct tgtatttaat attaatgata
      901 agaatggttg tgatgacgca tatctgaaaa agattataag ctattttggg cagttgcaga
      961 atgaggctgt tcatgtgacc agctgctacg aaacacttgc ggtgtacatt tgtctgataa
     1021 ggaaaaataa aattaatgat gtgctgcagc attatgatga catggaaaga aaaggattgt
     1081 ttggtgaatt accttcagga tatgtcaggg gggcattatc gttgcttcgg acagcactgg
     1141 aggttaaagt aaatagaaaa aatataaagt atggctcgtt gttttattgg cttgaccatg
     1201 taaaagccta tcaggatgca tttattgaaa aaataccact tattgatcct gtatataagg
     1261 agggtgaaat acagtatgat gcgaacaatt ttaccctgat gcgtgttatt aagatgtata
     1321 attgtatgct tgaaaagatc agtacgaaac catatattgc gcccccatat attacaggtt
     1381 tgcttgatga cgtagagaag gtgctggata agataaatat tctgatagat aaagagtatg
     1441 tttatgatgg taagacgctg gcagaagtca ttatggaaaa caaagtatta tccagtcgcg
     1501 aacgtaaaga aactatgata gggcttttca caggcagtaa gaaatatact ttactgcaat
     1561 gtgttgagaa gcttggtgta ttagttcact atgtgaaatc accggtcgat gaaattaaaa
     1621 atgtgatgat gttgtatggc gataaggccg aaaacaggaa cagacgcaga atgatatatg
     1681 atgcgttaac cattatttgt gaagatgaca tcaggaataa ccctcctgag ctgtcgtgaa
     1741 ttcatctgag tgtgttttgg gtcaataatc tttatatacc ctgctaattt tgctaaccat
     1801 tagcaaaatt agcaggggta ttatctatgt ctggcaagag aaaattctat aatagtatga
     1861 tttatttgtt tgttttgttt ttcaattaga atggtgtgtt tttgtgatct atgtctaatt
     1921 gttttgtatt cttagcgcca tcccattcat gggcagatgt acataccgcc cctattagat
     1981 tgatgggttg catgttcatt gtatctgtaa aatatgccag ttttgacatt aatacataat
     2041 ctgtaattcg gtgtaagctc ttgtctggtg aattatgttg cgtattccgc tctattcgat
     2101 cacggattgg gccaaatcat gatcgattcg aatgccaaac tgcgatcgat tcagatgcca
     2161 ttttgcatta tgttagtggc ttattcttct tgttcttttt tacaggttat gtaagtgtga
     2221 ttatcctgat tactggtcac tcattatata ctgagtcgac aaaacaaatg agtattaact
     2281 gaagttttta tacagtgatc atctgtctgg ttaggttagt tagttccggg agaatcacat
     2341 ttatttcata taccataaat aattaaggtt aattagtatt attagttgga gttatggtat
     2401 tgtgtgtctg tgccgtattg tttgtatgtt tgttgtataa cttatctctt tacgtggtaa
     2461 tgtcactcct ctttatgcta agtattctgt atgtgtgtaa tgcatatatc tccatacact
     2521 acgcagtgaa gcatatgggg gaagctgtat ggtgaattat gtgtgtaatg ttgtcctgta
     2581 cgtacttaac actggttacg tatcggaaca ctattagagc gtgttatgac tccgttacag
     2641 tttataggta taggtaaata tgatcttttc tgtatggaac aatcttctta tggtgtgtgg
     2701 gtatgttctg tataccatta ttcttttatg ctaatgatta aatacccgtg gtctctctcg
     2761 gctttactta atggttcgac aatattaata gcgatgttgc ctgtagctag ccgatactga
     2821 gttttgtgaa tgtcagatta cttcctgtaa agaatttagt ggtgcttcct gaaactcaat
     2881 tctatgaggt gtgtttttcc attgtaattc gtactggttc tcagtttaag agagtctcac
     2941 tgtattctga agtattacct catcacagta atgcggtaat acaaaacagt tttatcaggt
     3001 tgtctgtgct gctggtttac ttcgtttatt aatgccgtgg tacggcgttt ctctactctc
     3061 agtatttacg taattctctc ttcctgtgag ttttataaag taaggactat aaaatgtata
     3121 atgccaatcc caattacaaa atggatttca tgattctgaa agacgtgaat gaacacatgg
     3181 atggtatgtt tcaacgtttt tctaaactgt tacctttcag aattgatttt gcttacagga
     3241 aagacacgcc cagtttcggg cattcctgta agcacagtat gtgtatggaa atataccgtt
     3301 tacttagcga aacccagacg atgttggcgg gatattactg ggtaatggaa tatacaccag
     3361 acaaagggct tcatattcat tttatcggct acctggatgg acaacgtcac aagaactctt
     3421 atcagatatc caggcaactg ggagatatct ggaggcggat cacggaaggg gagggttatt
     3481 ttcatctgtg ccgggctaaa gacaaatacc cggtgcgtat cgatcacgtg attcactata
     3541 gtgataaatc tgcggtagac gacttacgtt acgcccttag ctatctggct aaacaggatc
     3601 agaaagaaca cggtattatt ctgggccgta gcaggcttcc cgaaaaaagt aaccggggtc
     3661 gaccacgaca taactgaatt atgggggctt atgtccgaac tatcgtaagt tatcgcgtgg
     3721 atttgccttc acacgaattt cagtcgtggt gccgccggga gacaggtaca agtgataacg
     3781 catatggttt ataaatctga cagcgatgaa ctagcagtac tgcgggcaga aaatacccgg
     3841 ctggtctcat tacttgaagc tcatgggatt gaatggcgac gtaaaccgca gagccctgtg
     3901 cagtgcgttt tcgtattatc cacggatgag aaagttgcgt tatttcgtcg gttgtttcgt
     3961 gggcgtgatg atgtatgggc ccttcgatag gaaagcaaaa ccagcggcaa atcagggtat
     4021 tcgtctacct ggctaacgaa tggctgttgg gaatatgcag taagccctgg ataaaatgtg
     4081 gggactgcgc tcaccgtcag ttgatccctg tatctgatct catcatctac ctgaggtagt
     4141 gtacaatatg ccgatatcga actgatactt aattaaaata tctagagtgt gatatatctc
     4201 tgagaactat caatacgaat attaaaaaca cttccagaat aacagcaata cggggggatc
     4261 tccattaccc tcctgtgatt gacaacgggg atatcattac ctgtttccct aacctgatcc
     4321 ccggagagat atctcgtttc cgtaactcgc tgaatgcgaa aattaatgca gatcaacacc
     4381 gtaaaaaaca acaaggcaaa cgcacctacg caacgtcggt ttgtattatc tgggcgaaag
     4441 aatatacgca gtcctttaaa tgtcattatc acgtctgcct ttttttaaca aagacgccta
     4501 ttaccatctg ggaaactatg agcgctctga caccctgaga gggttaatta ccggagcctg
     4561 gtacagtgcc ttagggttgg aactggaaga tcaccaggga ttagttcatt tccctgctaa
     4621 tggacgttac atcctggata ttaacgatcc taatttccca gagcagtacc agcgcttatt
     4681 ggtacgtctg gattatttga cgaagctcga cactaaagca tcaggccagc gtaactttgg
     4741 ctacagcggt ttttgaccta ttgtaactat aaggtgattt attatgtata cagaatcaaa
     4801 cattaagctt tcctgtaacg ccctctggca aaagtgtgtc cttgcaactc tgtatcgggc
     4861 tgctgctaca catcctaaaa ttaaattatt acagtttaaa ttaatacctt gccctggtat
     4921 gaataaggca attccttctc tgattgctga ttttactggt gagctgcata gactaatttc
     4981 tgaacatttg cagtgtaagc gacgtaaaga taaatcagtc gagcccaccc ctttatatta
     5041 cctctggagt agggaagctg tggctaacga tgccactcct tatcttatgg tgctgctact
     5101 taataacgat gtctggtgta a
//
LOCUS       DAZXUL010000213         5102 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZXUL010000213 DAZXUL010000000
VERSION     DAZXUL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.214"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            393..2306
                     /gene="mtlA"
                     /locus_tag="ACH3L1_004170"
     CDS             393..2306
                     /gene="mtlA"
                     /locus_tag="ACH3L1_004170"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFT4078657.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            2401..3549
                     /gene="mtlD"
                     /locus_tag="ACH3L1_004171"
     CDS             2401..3549
                     /gene="mtlD"
                     /locus_tag="ACH3L1_004171"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFT4078658.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3549..4136
                     /gene="mtlR"
                     /locus_tag="ACH3L1_004172"
     CDS             3549..4136
                     /gene="mtlR"
                     /locus_tag="ACH3L1_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFT4078659.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4148..4357)
                     /gene="yibT"
                     /locus_tag="ACH3L1_004173"
     CDS             complement(4148..4357)
                     /gene="yibT"
                     /locus_tag="ACH3L1_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFT4078660.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            4641..5003
                     /locus_tag="ACH3L1_004174"
     CDS             4641..5003
                     /locus_tag="ACH3L1_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFT4078661.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1282 a         1272 c         1333 g         1215 t
ORIGIN      
        1 tacataacga ttgtatgacg aaggcataac atgctgtaga tcacatcagg tgaacgccgt
       61 aagaaaatat cttgtgattc agatcacaaa gattcaacaa accatcaaaa caaaaatgtg
      121 acattactca catttaaatg ccatttttag cgaaaatcac cgccttgttt cttttttata
      181 caggcgtttt gtgatgagcg tcacgtcaat tacctctcta ccccctatat ttatgtgact
      241 gatatcacac aaaagcccgt cgactggaca gttaaccgat tcagtgccag atttcgcagt
      301 atctacaagg tccggctacc tctgccgcca cattaacaaa aaacctcggg cttccagcct
      361 gcgcgacagc aaacataaga aggggtgttt ttatgtcatc cgatattaag atcaaagtgc
      421 aaagctttgg tcgtttcctc agcaacatgg tgatgccaaa tatcggcgcg tttatcgcgt
      481 ggggtatcat caccgcgtta tttattccaa cagggtggtt accgaacgag acgctggcga
      541 agctggtcgg gccgatgatc acttatctcc taccgctgct gatcggttat accggtggta
      601 agctggtagg cggcaaacgt ggtggcgtag tcggtgccat taccaccatg ggcgttatcg
      661 tcggcgcaga catgccgatg ttcctcggtt ctatgattgc aggtccgctg ggcggctggt
      721 gcattaagca cttcgaccgc tgggtagacg gtaagatcaa atccggtttt gagatgctgg
      781 tgaataactt ctccgcaggc atcatcggga tgatcctcgc tattctggca ttcctcggca
      841 ttggcccgat tgttgaagcc ctgtccaaaa tgctggctgc gggcgttaac ttcatggttg
      901 tccatgacat gctgccgctg gcgtctatct ttgttgaacc ggcgaaaatc ctgttcctca
      961 acaacgccat taaccacggt atcttctcgc cgctgggtat tcagcagtcc catgaactgg
     1021 gtaaatcaat cttcttcctg attgaagcta acccaggtcc aggtatgggc gtgctgctgg
     1081 cgtacatgtt ctttggtcgt ggtagcgcta aacagtctgc gggcggtgcg gcaatcatcc
     1141 acttcctggg tggtatccac gaaatctact tcccgtatgt gctgatgaat ccgcgtctga
     1201 tcctcgcagt catcctcggc ggtatgactg gcgtgttcac gctgactatc ctgggcggtg
     1261 gtctggtttc tccggcatct ccgggttcta tccttgctgt actggcgatg acaccaaaag
     1321 gtgcttattt cgctaacatc gcgggtgtgt gtgcggcgat ggctgtctcc ttcgttgtct
     1381 ctgctatttt gctgaaaacc agcaaagtga aagaagaaga tgatattgaa gcagcaactc
     1441 gtcgtatgca ggacatgaaa gctgagtcta aaggcacatc tccgctatct gcgggcgatg
     1501 tgactaacga cctgagccac gtacgtaaaa tcatcgttgc ctgtgacgcc ggtatgggtt
     1561 caagtgcgat gggcgcaggc gtgctgcgta agaaaattca ggatgcaggt ctgtcgcaga
     1621 tttcggtcac taacagcgcg atcaacaacc tgccgccaga tgtggacctc gtcatcactc
     1681 accgtgacct gaccgaacgc gctatgcgcc aggttccgca ggcacagcat atttcgctga
     1741 ccaacttcct cgacagcggc ctgtacacca gcctaaccga acgtctcgtt gctgcccagc
     1801 gccatactga aaacgaagtg aaagtgaaag acagtctgaa agacagcttt gacgattcca
     1861 gtgctaacct gttcaaactg ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca
     1921 aagaagaagc gattcgtttt gctggcgagc agctggtgaa aggcggttac gttgagccgg
     1981 aatacgttca ggcgatgctg gatcgtgaaa aactgacccc gacttatctg ggtgagtcta
     2041 tcgcggtgcc acacggtacg gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt
     2101 tctgccagta cccggaaggc gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg
     2161 tgattggtat tgcagcccgt aacaacgagc acattcaggt tatcaccagc ctgaccaatg
     2221 cactggatga tgagtccgtc atcgagcgtc tggcacacac caccagcgtg gatgaagtgc
     2281 tggaactgct ggcaggtcgt aagtaatcca atcctaccct ctcctcacgg agagggtttg
     2341 ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa cattgatgaa ggttaatact
     2401 atgaaagcat tacattttgg cgcaggtaat atcggtcgtg gctttatcgg taaactgctg
     2461 gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc aggtggtact tgatgccctg
     2521 aatgcccgtc atagctatca ggttcatgtg gtcggtgaaa ccgagcaggt agataccgtt
     2581 tccggcgtca atgctgtcag cagcattggt gatgatgtcg ttgatctgat tgctcaggtc
     2641 gatttagtca ctaccgccgt tggcccggtt gtgctggaac gtattgctcc ggctatcgcc
     2701 aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc cgctgaacat catcgcctgt
     2761 gaaaacatgg tacgcggcac cacgcagctg aaaggtcatg tgatgaacgc cctgccggaa
     2821 gacgccaaag cgtgggtaga agagcacgtt ggctttgtcg attccgccgt tgaccgcatc
     2881 gtaccgcctt cggcttcggc aactaacgat ccgctggaag tgacggtaga aactttcagc
     2941 gaatggattg tcgataaaac gcagttcaaa ggcgcactgc cgaacatccc aggcatggag
     3001 ttaaccgaca acctgatggc atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat
     3061 gctataaccg cgtacctcgg aaaactggcc ggtcatcaga ccattcgtga cgctattctc
     3121 gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc
     3181 aagcgctacg gctttgacgc agacaaacat gcggcgtaca tccagaaaat cctcggtcgt
     3241 tttgagaacc cgtatctgaa agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa
     3301 ctgagtgctg gcgaccgtct gatcaagcca ctgctcggta cgctggaata cagccttccg
     3361 cacaagaatc tgattcaggg gattgctggt gcaatgcact tccgcagtga agatgatccg
     3421 caggctcagg aactggcagc actgatcgct gacaaaggtc cgcaggcggc gctggcacag
     3481 atttccggtc ttgatgccaa cagcgaggtt gtatccgagg cggtaaccgc ttataaagca
     3541 atgcaataat ggtggaccag gcgcaggaca ccctgcgccc gaataacaga ttgtcagata
     3601 tgcaggcaac aatggaacaa acccaggccg ttgaaaaccg tgtgcttgag cgtctgaatg
     3661 ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga gcttttgacc gaggcggtaa
     3721 atcttctggt gcttcaggta ttccgcaaag acgattacgc ggtgaagtat gctgtagaac
     3781 cgttactcga cggcgacggt ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg
     3841 ggttgggcgt cattaaccgc caggaatacg aagatgcgga actgctgatg gcattgcgtg
     3901 aagagctaaa tcacgacggc aacgagtacg cctttaccga cgacgaaatc cttggaccct
     3961 ttggtgaact gcactgcgtg gcggcgttac caccgccgcc acagtttgaa ccagcagact
     4021 ccagtttgta tgcaatgcaa attcagcgct atcaacaggc tgtgcgatca acaatggtcc
     4081 tttcactgac tgagctgatt tccaaaatca gcttaaaaaa agcctttcaa aagtaagcaa
     4141 cgtctgctta ctgcccctct acctgcttcg gccgataaag ccgacgataa tactccagac
     4201 gttgtagata taacggcacg ctttcatcgg gtattccgga cggaatcgcg ttacggggag
     4261 gaagtttttt cagatactcc cggaacgcct ggcttgatgc catgaaatct acggctttat
     4321 cgataagaag cggaacgttt tcacctaatt tgcccatgat gatatctccg tattaccccg
     4381 cgccgggaat gcgcggccgc caattttagt ttaggttccc ggtaaagatg acattaagga
     4441 aaacgtgctg aatcctcaat aatcatccgc tgctgtttat tatctttttc tatagatttc
     4501 ttaattaatc aacgaattgc gtagtttttg agcatgttaa tgattacgtt gcagaactat
     4561 tacaatgtca tcagttgtga acaaagcacc tggtcgcgca tactaggggc tataaattta
     4621 tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaag cgtctcagcg
     4681 atcgcctgga cgccatccgc caccagcagg ccgatctgtc gctggttgaa gccgcagaca
     4741 aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgct cgcctgcgtg
     4801 aggttcatag ccaaaaactg agtaaagaag cacagaagct gatgaagatg ccgttccagc
     4861 gcgcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt gttcgcggac
     4921 tggtggttgt gcacccaatg accgcgctgg ggcgcgaaat gggcctggaa gagatgactg
     4981 gattttctaa gactacgttt taagcccctc agacgactgg ttcgatcgta tcaacccgca
     5041 tctggcttgc cttagctggt caatgcgggg actctcctgt ctactcgcct aattaccaaa
     5101 ca
//
LOCUS       DAZXUL010000214         5023 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZXUL010000214 DAZXUL010000000
VERSION     DAZXUL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.215"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="ACH3L1_004175"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="ACH3L1_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFT4078662.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="ACH3L1_004176"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="ACH3L1_004176"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFT4078663.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="ACH3L1_004177"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="ACH3L1_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFT4078664.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="ACH3L1_004178"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="ACH3L1_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFT4078665.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5023
                     /gene="yidZ"
                     /locus_tag="ACH3L1_004179"
     CDS             4378..>5023
                     /gene="yidZ"
                     /locus_tag="ACH3L1_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFT4078666.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1255 g         1416 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cct
//
LOCUS       DAZXUL010000215         5014 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZXUL010000215 DAZXUL010000000
VERSION     DAZXUL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.216"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..297
                     /locus_tag="ACH3L1_004180"
     CDS             <1..297
                     /locus_tag="ACH3L1_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFT4078667.1"
                     /translation="LFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADN
                     QQVKLSMPELEELAAAMVQAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(498..1355)
                     /gene="sitD"
                     /locus_tag="ACH3L1_004181"
     CDS             complement(498..1355)
                     /gene="sitD"
                     /locus_tag="ACH3L1_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFT4078668.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="ACH3L1_004182"
     CDS             complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="ACH3L1_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFT4078669.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="ACH3L1_004183"
     CDS             complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="ACH3L1_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFT4078670.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3033..3947)
                     /gene="sitA"
                     /locus_tag="ACH3L1_004184"
     CDS             complement(3033..3947)
                     /gene="sitA"
                     /locus_tag="ACH3L1_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFT4078671.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4379..>5014)
                     /locus_tag="ACH3L1_004185"
     CDS             complement(4379..>5014)
                     /locus_tag="ACH3L1_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFT4078672.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1323 a         1227 c         1200 g         1264 t
ORIGIN      
        1 ctgttcacgt ttgaatggaa cggtcgtatc tggaatgctg gtcccgactc actgggccgc
       61 ctgtccccgg tagtcatgct ggcaaaatct gtcacagcac aaacacatat ggcgtggagc
      121 gatgccgata atcagcaggt gaaactgtcg atgccggaac tggaagaact ggcggcagca
      181 atggtgcagg cgcaggtcga tcgcaacgat gagatttatc gccgtcagcg ggagctgaag
      241 gaagagctga atagtctgaa ggatttgaat tcggttagga attttatcgt ggaataacag
      301 aagctgcggc acgtcgtatg caggaacgtg ccgcggttgg ctggtaaact ttcgatagtg
      361 cgagtattga atgatttcta ggcgttatcg attttacgta ttttttgcat gatagaattc
      421 gtacctcctc ccaacgacct tccatggctt tccggacttc tgtaggctgg cggacgatgc
      481 tgtcctcgga tgttgtgcta attgggtgat aacagatcct gtgtatgggc gtttcccttt
      541 gtgcgtgcgt tgatgctggt aacggtaaac gtcatgatga acacgaccgc gaataagacg
      601 acaatcgtcg gagctggtgc gctgtcgata aaaaaggaca gccagacacc agtcattgac
      661 actattaccg atatgccagt cgccagtagg agcgcaatat gaaagcgttg tgtcaggagt
      721 actgcaatgg cgccaggcgc aatgagcaga gaaatagaga gaatgatacc tactgctttc
      781 agcgtcgcca caatggtcag ggaaaccatg cacagcagcc cataatgcag ccatctcgta
      841 tgcaaaccgc ttacctgcgc ctgttgataa tcaaagctga acagcaaaaa atcccgccat
      901 tttacgctaa taacaagtgt aaccagcccg gcaataatca ccgtctggat tatgtcgccg
      961 atggttatcc cgagcatgtc gccgaaaaga atatggtcaa gatgtacgtc tggtttaatt
     1021 gcaatataca agatcagacc tgcggcaaac atgccagaaa aaacaatccc cataactgta
     1081 tcttgcttga ttcggctgtt gtctttcagg tatcccgtcg ccaccgcgca gaatactccg
     1141 gcgacgaatg cccctgtagc cagaggtaac cccagaatcc aggtaagcac gatgccagga
     1201 aaaaccgcat gactcatcgc gtcacccatc agtgcccaac ctttcaggac cagaaagacc
     1261 gacagcagcg cacagggtat ggaaactacc agtgaaatca gcagtgcgtg actcataaag
     1321 gtgaactgca gtggctccag taataatgcc atcatgagcg atcctccagc gctttatgcg
     1381 cacgccgacg atttgccaaa agtccatggg tgggcgcaaa tacaaatgct aaaagaaaca
     1441 gcagtgtctg cgccaccacg atgattccac ctgtcgcgcc gtccagaaag taactgaccc
     1501 acgcgccgag gaaactggtt acactgccaa tagttacagc aattatgagc aggcggggaa
     1561 aacgatcggt taacaaccat gctgttgcac cgggagtcac caccagacag atcaccagaa
     1621 aggcaccaac cgtttgcagc gccgcaacgg tcgaaaccga cagcagggtg aagaagagga
     1681 tttttaatct gcccggatgt aatccaatag cacgggcgtg attctcgtcg aaaaaggtca
     1741 ccataagatc tttccatttg aaaaaaagca cgatgatcga gagaatgcca atgatagtga
     1801 gttgcaggat atccgccgga tcgattgcca gaatattgcc caggacgatg gtctgaatgt
     1861 tcacggatgt cgggttcaat gacaccataa acagccccag gccaaaaaag gacgagaaga
     1921 tcaggccgat gatggcatct tcttttaagc gggtgcgttg gttaagaaac agcatgctgc
     1981 ccgctgccag tccgccggaa aagaaggctc ccagagaaaa cggaagcccc agcatatatg
     2041 cccccgcaac acccgggaca atagagtgcg acagcgcatc gccaatcagc gaccagcctt
     2101 ttagcatcag atagcatgac aggaaagcgc agagaccgcc gaccattgcc gagacccaca
     2161 tcgcattgag catatattca tagctgaagg gttccagtag cacgttcatc ttgcttccct
     2221 ctgcaccgct gacggtcgat gtgccacaaa agggcgttca tcgtcagtaa taatgctttc
     2281 ttccgagccg ttaagtgtga catggcgcag tacgccgcta aaagccagct ccaggttctc
     2341 ggctgtaaaa gtggtgtctg tcggcccgct tgccaggacg gtgcctttga ccatgaccgt
     2401 ataatcgcaa aatgtcgtga ctgagccgag attgtgcgtt gagacaagca tcgttttgcc
     2461 ttccgcgcgt aactccctca acaggctgat gattttcgct tcggttttca catcaacgcc
     2521 ggtaaaaggt tcatccagca ggatcacatc accctgctgt gcaattgctc tcgccagaaa
     2581 gacgcgtttc ttttgtccac cggaaagctc gccgatttga cgatggcgaa aatccaccat
     2641 atcgacacgt tccagcgcat cggtgacaat ctgacgatcc ctctttttgg ctatacgcaa
     2701 catacccata tgcccatagc ggcccatcat caccacatct tccaccagta caggaaatga
     2761 ccagtcaacc tcttctgact gagggacgta ggcaaccagg tttttctgta gcgcctgtcg
     2821 cgtgggaata cccagaacag atatctttcc gctggtcaga cgcacaaatc ccataatcgc
     2881 tttaaacaac gtcgattttc cggaaccgtt taccccaacc agagcggcaa tagatccact
     2941 tggtactgtg aaggatgcat cgcgcagtgc tgtgtgcccg ttacgccagg tgacagtaac
     3001 atcattaacg acaatgcctg cagattgcat cattatttct cccttttccc ggctttaatt
     3061 ccctgtacca gcgtactggt agtaacctta agaaggtcga tatacgtagg taccgggccg
     3121 ttttctgtgc tcagggaatc gacatagagc acaccgccgt agtgtgcgcc ggtttcacgc
     3181 gcaacctgac gcgctggttt atcggaaatc gtactctcgc taaagactgc cgggatatga
     3241 tttttcttaa ctatatcaac aaccttacgt acctgctgcg gtgttccttg ttgatcggca
     3301 ttaatcggcc acagataaag ctcttttagc cccaaatcgc gtgcgagata agaaaaagcc
     3361 ccttcactgg tgaccatcca tcgctgattc tcagggagtt ccgtaatctg cttacgcagg
     3421 ggggcaaggg tttgggtaat cttggcttta taagtatcgg cattacgttg gtaggtttgt
     3481 gcatttgccg gatcgtattt tatcaacgca tcacgaatat tatcgacgta aatcagagca
     3541 ttatctggcg acatccatgc atggggatta ggtttgccct catagggtcc ttcggtgatc
     3601 cctactggcg tcacacccga agagacaatt acttctggaa ccccattgag atgctggtaa
     3661 aagcgttgga accacaattc cagattcata ccattggcga gaatcagttg tgccccctgc
     3721 gcacgtttaa tatcgccagg ggtaggctga tactcatgaa tttctgcacc aggcttggtt
     3781 atggatgaga cttctgcagc atctccagcc acgtttttgg ccatatccgc gatgatggta
     3841 aatgttgtaa tgaccttgaa tttttcagtt gcacttgcct gaaatgcgat cgagcaggtg
     3901 agggcgaggc accccaagag catggttact ttttttattg agtgcataat ttagtaccta
     3961 tagtgagcaa aattagaaca aacatagcaa cggctatgtt ttatagcata acctatacaa
     4021 aatgaaaata attatcatta acagctttga ggcctaaaac agggattagg ttgtttcctg
     4081 ctggcgacat gatattgagg aaaaaagtag agcagctatt aggctttgaa atgactttat
     4141 gaggaaaatt gttgatggca aaaatggggc aaaaaagagt gggttatcgt agcttattgt
     4201 tgtcgctgat aatgtttaac atattgaaaa ataaataaaa tacttatgag tcagatagtt
     4261 gtgatttttg cccttacttg ttcaggttgt attgttcttt ctttgtaatt tgttgatttt
     4321 cttgcattat ttcagttctc tggtactaaa tggggcaaat tgggggcaaa ctttgcaact
     4381 acgataaccg cgcattcaac atggctatct gttcgtcgtt catgtcatca atccacatac
     4441 cgtaaatttc atacaccatt tgcgcagttt catgccccat ctggctggct ataaatgccg
     4501 ggttcgctcc tgccgtcaac agccagcagg caaaagtatg tcgcgtatgg tacggattac
     4561 ggcggcgaat accagcacgt tttactgctg cattccatct tgtacccaaa ctgcttaccg
     4621 agtaataagg tttctgtttt tcgttacaca ccctgggcat gaaaacaaaa tgcagttttt
     4681 gtttttcagt tctgccgtac tcccgatgat aaaaagtgat ttcgcttttg cgatgatgcc
     4741 cggtcagttt gtattgctcc ttcagtgctt caagagccgg ctgcagtaat gctaccgtcc
     4801 ggatcccggc atttgttttt gggggaccga acatatcaag tatcgtcagg tttcttctga
     4861 cattcacaat tcccttctcg aaatccacat cctcccacgc cagagctgcc agttccccgt
     4921 gacgaagccc ggagtaaacg gcaaatttcc acaagttttg gctctgtcct ttttcacttt
     4981 ccattaatgc attgaattct gttttagata acgg
//
LOCUS       DAZXUL010000216         4973 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZXUL010000216 DAZXUL010000000
VERSION     DAZXUL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.217"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACH3L1_004186"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACH3L1_004186"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFT4078673.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACH3L1_004187"
     CDS             1020..1703
                     /locus_tag="ACH3L1_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFT4078674.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACH3L1_004188"
     CDS             1700..2161
                     /locus_tag="ACH3L1_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFT4078675.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACH3L1_004189"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACH3L1_004189"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFT4078676.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACH3L1_004190"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACH3L1_004190"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFT4078677.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACH3L1_004191"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACH3L1_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFT4078678.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACH3L1_004192"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACH3L1_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFT4078679.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..>4973)
                     /locus_tag="ACH3L1_004193"
     CDS             complement(4903..>4973)
                     /locus_tag="ACH3L1_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078680.1"
                     /translation="NYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1177 a         1305 c         1238 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctgact caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAZXUL010000217         4862 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZXUL010000217 DAZXUL010000000
VERSION     DAZXUL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.218"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..527
                     /gene="ydcF"
                     /locus_tag="ACH3L1_004194"
     CDS             <1..527
                     /gene="ydcF"
                     /locus_tag="ACH3L1_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFT4078681.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="ACH3L1_004195"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="ACH3L1_004195"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFT4078682.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACH3L1_004196"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACH3L1_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="ACH3L1_004197"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="ACH3L1_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFT4078683.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="ACH3L1_004198"
     CDS             4169..4342
                     /locus_tag="ACH3L1_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078684.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4368..4436
                     /gene="yncP"
                     /locus_tag="ACH3L1_004199"
     CDS             4368..4436
                     /gene="yncP"
                     /locus_tag="ACH3L1_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFT4078685.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACH3L1_004200"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACH3L1_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFT4078686.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="ACH3L1_004201"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="ACH3L1_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1245 a         1119 c         1207 g         1291 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aa
//
LOCUS       DAZXUL010000218         4743 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZXUL010000218 DAZXUL010000000
VERSION     DAZXUL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.219"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            240..2672
                     /gene="hsdR"
                     /locus_tag="ACH3L1_004202"
     CDS             240..2672
                     /gene="hsdR"
                     /locus_tag="ACH3L1_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFT4078687.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="ACH3L1_004203"
     CDS             2739..4208
                     /locus_tag="ACH3L1_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFT4078688.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..>4743
                     /locus_tag="ACH3L1_004204"
     CDS             4208..>4743
                     /locus_tag="ACH3L1_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFT4078689.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFD"
BASE COUNT         1374 a         1154 c         1142 g         1073 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttt
//
LOCUS       DAZXUL010000219         4689 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZXUL010000219 DAZXUL010000000
VERSION     DAZXUL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.220"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            133..1149
                     /locus_tag="ACH3L1_004205"
     CDS             133..1149
                     /locus_tag="ACH3L1_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISShdy1 family transposase"
                     /protein_id="HFT4078690.1"
                     /translation="MTVVTVGIDLAKNVFAVHGVNAAGKAVLVKPLVRRAKLLELIAT
                     LPACLIGIEACTGAHFWAREFQKMGHTVRIMAPQLVSPYRMGGRHGKNDAADAAAICE
                     AVTRPNMRFVPIKSVEQQGQLFVHRAHQGYVSERTALINRIRGLLSELGIVLPQAFKA
                     FLSGVAQVLEDLPGYCNQVIGDLLNELYHIEEKIKNSEKLIQQLARDNSAAQLLMQFR
                     GIGPMTATAIVASIGNGHDFKNGRQFSAWLGLTPRQQSSGGKARLGRITKAGDSYLRT
                     LLVLGARSVLLGAAKNDDPVSRWALQLKERRGYGRALVAIAAKNARMCWAMLHLRDEF
                     RFPA"
     gene            1696..1770
                     /locus_tag="ACH3L1_004206"
                     /pseudo
     CDS             1696..1770
                     /locus_tag="ACH3L1_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1846..2529
                     /locus_tag="ACH3L1_004207"
     CDS             1846..2529
                     /locus_tag="ACH3L1_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFT4078691.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLTAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            2530..2751
                     /locus_tag="ACH3L1_004208"
     CDS             2530..2751
                     /locus_tag="ACH3L1_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078692.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            2681..3199
                     /locus_tag="ACH3L1_004209"
     CDS             2681..3199
                     /locus_tag="ACH3L1_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFT4078693.1"
                     /translation="MAPIACSWKRIPKFWNWIPASKIEKETRMYGTCETLCRALAAKY
                     PGDTPLMLVIWSPEEIQALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSGAAM
                     EIINNVRENRQVTVPAELLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVNQA
                     RALLKNNTREND"
     gene            3244..4014
                     /locus_tag="ACH3L1_004210"
     CDS             3244..4014
                     /locus_tag="ACH3L1_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078694.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIYVL
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGLACDVLR
                     IPDEPSRRWFDRDILREVVLEARTEIRSMADALAKIRK"
     gene            4425..>4689
                     /locus_tag="ACH3L1_004211"
     CDS             4425..>4689
                     /locus_tag="ACH3L1_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFT4078695.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAA"
BASE COUNT         1062 a         1184 c         1322 g         1121 t
ORIGIN      
        1 ctgatgaacg cccttcaagc cgctttcgcg aatgaaaggg acatcttatt cggtctttac
       61 gctggggagt tactttggaa gactgcgtca acacgatgat aagcgtacaa agccattgtt
      121 aagggatcct ctatgactgt tgttactgtc ggtattgatt tagctaaaaa tgtgttcgct
      181 gtgcatggcg ttaatgctgc aggaaaagct gttttagtta agcctcttgt gcgtcgggct
      241 aaattgcttg aattgattgc aacacttcca gcatgtctga ttggaattga agcatgtaca
      301 ggtgcacatt tctgggcaag agagttccag aaaatggggc atacagttcg aattatggcg
      361 ccgcaactgg tttctccata ccgtatgggt ggccgtcatg ggaaaaatga tgctgcagat
      421 gccgctgcca tttgtgaagc tgttacccgt cctaatatgc ggtttgttcc cataaaatca
      481 gttgagcagc aagggcagtt atttgtacac agagctcatc agggatatgt cagtgagaga
      541 acagcattga ttaatcgcat ccggggattg ctcagtgagt taggtattgt tctcccgcag
      601 gcgtttaaag cgtttttatc cggggtggct caggtccttg aggatttgcc cggttactgt
      661 aatcaggtta ttggtgatct tcttaatgag ctttatcaca tagaagaaaa gattaaaaac
      721 agtgaaaaac tcattcagca acttgccaga gataactcag cagcacaact actaatgcaa
      781 ttcaggggga taggcccgat gactgcaaca gcaatcgttg ccagcatagg taatggtcat
      841 gatttcaaga acggacgtca gtttagtgcc tggctggggc taacaccaag acaacaaagt
      901 tccgggggga aagcacggtt agggagaata actaaagccg gcgacagcta tctcagaaca
      961 cttcttgttt tgggggcgag atctgtattg ttgggagcag ctaaaaatga tgatccggtc
     1021 agtcgatggg ccctgcagtt aaaagaaagg cgaggatatg gacgagcgct tgttgcgata
     1081 gcagcaaaaa atgcacgtat gtgctgggca atgttgcacc tgagagatga attcagattt
     1141 cctgcctagt ctactgatta ttaatgagtt tatgaaattg atttctttcc ttgcatgacg
     1201 actgcacgat gatgatttaa aaggtttgga cctgcgccat ggtttactcg agatatgcaa
     1261 gaggctgcca tgtttatgtg gatatctgtt tcgcggatgg agtctatggc gtgcgtcttt
     1321 cgtcagggtt cgttgctaac aacagcacgt taataaccgt ttataggctc gctgtccgta
     1381 accttttact ctgactgtgc aagttgtgtg cagaaatcaa tctcatgata gataaaagaa
     1441 ttattccact gttgcacctg gagtgttcgg tgctcgcttt gctgcgctcc gcctcaatcc
     1501 agttgcaaca gcgtttaatt aaaagcatta aggcttgtgt cattgcgtac ttgagctatg
     1561 caggagattt ttattggttc tctgtttcgc ggatgaagtc tctgacatga gcctttctct
     1621 cataaaaatc gttctatttc atattgatat attaatttca tcatcatcgt tgactctaca
     1681 acagatgtcc ttataggcat agccttgccg tcaaaaagac gacggctgcg gtattccacg
     1741 gtcggcctga cccgttaccg gacgcggtga acagcccaca ggcagcgggg aacgggcacc
     1801 acaggggtgc cctcccggtg ccctctgtaa gagaaggagt ttggtatgtc ccgttttgtc
     1861 ctcggtaact gcatcgatgt tatggcccgt atccctgata acgccattga tttcatcctc
     1921 accgacccgc cgtacctcgt cggttttcgt gaccgtcagg ggcgtaccat tgccggcgat
     1981 aaaaccgatg aatggctgca accggcctgt aatgagatgt accgcgtact caaaaaagac
     2041 gcgttaatgg tgagcttcta tggctggaat cgcgtcgatc gttttatgtc tgcctggaaa
     2101 aatgcgggat tcagcgttgt tggtcacctg gtcttcacca aaaactacac atcgaaggcc
     2161 gcatatgtgg gctatcgcca cgaatgtgcc tacatcctgg caaaaggccg tccacgtctg
     2221 ccacaaaacc cgctgccgga cgtgctgggc tggaaatatt cgggcaatcg ccatcacccg
     2281 acggaaaagc ccgttaccag cctgcaacca ctgattgaga gcttcacaca tccgaacgcg
     2341 attgtgctgg acccgtttgc aggcagcgga tcaacctgcg tggccgctct tcagtccgga
     2401 cgccggtata tcggtatcga gctgcttgaa cagtatcacc gtgccggaca gcaacgactg
     2461 accgccgtgc aacgggccat gcagcagggg gccgcgaatg ataactggtt tgaaccggag
     2521 gctgcgtaaa tgaactatgc cggacacgaa aaacttcgcg ccgacgtggc ggaggtggcc
     2581 aacaccatgt gtgaccttcg ggccaggctg aacgatatgg agcatcgctg tcgctttgat
     2641 tccgatgtgc ttgttgaacg cctgacacgc cagacgctgt atcgcgccaa tcgcctgttc
     2701 atggaagcgt ataccgaaat tctggaactg gattcctgct tcaaagattg agaaggagac
     2761 gagaatgtac ggaacatgcg aaacgctctg ccgggcgctg gcagcaaagt atccgggaga
     2821 tacgccgctg atgctggtta tctggtcccc ggaagaaatt caggccctcg ctgacggaat
     2881 ggatatttcc ctgtccgatc atgaaatcag gactgtcctg gcgcgcctgg aggacatccc
     2941 ggaagaccag cggactgaat ccggtatttc ttccggtgcg gcgatggaga tcatcaataa
     3001 cgtgagagaa aaccgccagg tgaccgtccc tgctgaactg ctggcgtccc tgattcagac
     3061 cgccgaacag gcactgtgga aacgtgagtg ggccgcccgt gataacggcc tcgccgtccc
     3121 ggaatgcgtc acccgccgac aggcggtggt taatcaggcc cgcgccctgc tgaaaaacaa
     3181 cacacgcgaa aacgactgat gttatcgccg cctgcgggcg gcgattcagg gagaccatga
     3241 atcatgaacg aaacgctgaa tgcactgatt tgtcgccacg cccgtaacct gctgctggcg
     3301 cagggctggc cggaagagac ggatgttgac cagcgtaacc cgaagtatcc gggctggatc
     3361 agcatttatg ttctgctgga tgcgccccgg ctggcgacgt tacttatcaa ccgtcacggc
     3421 ggcgtactgc cgccgctcct ggcctctgcc attcagaaac tgaccggaac cggggcggaa
     3481 cttgttctgt ccggcagtca gtggcagtcg ctgccggtac ttccggcaga cggaacgcag
     3541 gtgtctttcc cgtatgccgg agaatggctg accgaagacg aaatcagggc agttcttgat
     3601 gcggtgcacg atgcggtacg cagtgtcagt tgccgggtgg cagaagatgc gcggcgtatc
     3661 agggcggcgc tgaccacaac cggtcagacg ttgctgaccc gccagacgcg ccgctttcgc
     3721 ctggttgtga aggaaagcga tcacccctgc tggctcgatg aagacgacga aaacctgccc
     3781 gtggtgctcg atgccatcct gaaccggggc gctcgttttt cgtcggtgga aatgtatctg
     3841 gtcagtgagt gtgtggagca tatcctgtcc agtgggctgg cctgcgatgt gctgcgtata
     3901 cctgatgaac cgtcccgtcg ctggtttgac cgggatattc tgcgggaggt ggtcctggaa
     3961 gcccggactg aaatccgcag catggcggat gcactggcga aaatccggaa atgatgacgc
     4021 tggcggggaa aaatctccgc cttttccagc cggtccccgg aaagagcaaa aggatgtcgc
     4081 cgcaggctcc gctttacccg gccatgcggg gcatggcctt gtgggttttc agttatgtgg
     4141 cctcagcgcc gtgtgcgggc tgtgccgtgc ctccatcgta gccgggcctg cagggttgca
     4201 aggtaaggtg aaccgctgcg gtcacccggt ccctccttcc cgtctgccgt gattttccgg
     4261 tcgttcaccc gctcagattt cgcggtctca cccttcaact cccgtcagcc gtgcgcgtca
     4321 ggctgcggct tcccttgcgc ccttgcaacc ctgcaggccc ggctacgatg gaggcacggc
     4381 accgcccggt gtcgcggaat atgtgaacaa cggaggaaaa catcatgcaa tatgcgaaac
     4441 ctgtcactct gaacgttgaa gagtgcgacc gtctgtcttt tctgccttac ctgtttggcc
     4501 tggattttct gtatgccgaa gcgtctgtat acgcgctggc gaaaaaaatg atgccggaat
     4561 atgaaggcgg attctggcac ttcatccgcc tgccggacgg tggcggttac atgatgccgg
     4621 atggcgaccg tttccacatg gtgaacggtg caaactggtt tgaccgtacc gtgagcgctg
     4681 atgccgcag
//
LOCUS       DAZXUL010000220         4651 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZXUL010000220 DAZXUL010000000
VERSION     DAZXUL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.221"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1482
                     /gene="lsrA"
                     /locus_tag="ACH3L1_004212"
     CDS             <1..1482
                     /gene="lsrA"
                     /locus_tag="ACH3L1_004212"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFT4078696.1"
                     /translation="KQYSGVNVLKGIDFTLHQGEVHALLGGNGAGKSTLMKIIAGITP
                     ADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLSIKENILFGLAKKQLSMQK
                     MKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSRILILDEPTASLTPAETER
                     LFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTIALSGKTSELSTDDIIQAI
                     TPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTGEGFRNVSLTLNAGEILGL
                     AGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGERLLRGLVYLPEDRQSSGL
                     NLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNIKFNQPEQAARTLSGSNQQ
                     KILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIAAQNVAVLFISSDMEEIEL
                     MADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQEASC"
     gene            1476..2504
                     /gene="lsrC"
                     /locus_tag="ACH3L1_004213"
     CDS             1476..2504
                     /gene="lsrC"
                     /locus_tag="ACH3L1_004213"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFT4078697.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            2504..3496
                     /gene="lsrD"
                     /locus_tag="ACH3L1_004214"
     CDS             2504..3496
                     /gene="lsrD"
                     /locus_tag="ACH3L1_004214"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFT4078698.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            3508..4531
                     /gene="lsrB"
                     /locus_tag="ACH3L1_004215"
                     /pseudo
     CDS             3508..4531
                     /gene="lsrB"
                     /locus_tag="ACH3L1_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            4558..>4651
                     /locus_tag="ACH3L1_004216"
     CDS             4558..>4651
                     /locus_tag="ACH3L1_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HFT4078699.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1031 a         1133 c         1298 g         1189 t
ORIGIN      
        1 aaacagtatt caggggtcaa tgtcctgaaa ggcatcgatt ttacgttgca tcagggggag
       61 gtccacgccc tgctcggcgg caatggtgcc ggtaaatcga cgttaatgaa gattattgcc
      121 ggtattaccc ctgctgatag cggtacgctg gagattgggg gcaacaacta cgccagatta
      181 acgccagttc atgctcatca gctgggcatt tatctcgttc cccaggaacc gctgcttttc
      241 ccaagcctgt cgataaaaga aaacatcctg tttgggctgg caaaaaaaca gctctccatg
      301 cagaaaatga agaacttgct ggcggcgctg ggctgccagt ttgatctgca tagtctggca
      361 ggatcgctgg atgtcgccga tcgccaaatg gtggaaatcc tccgcgggct gatgcgcgac
      421 tcgcggattc tgatcctcga tgaacctacc gcctcgctta cccctgcgga aaccgaacgc
      481 ttgtttagtc gcttgcaaga gctgcttgct actggcgtgg gtattgtttt tatctcgcat
      541 aagctgccgg aaattcgcca gattgccgat cgaattagcg tgatgcgcga cggaaccatc
      601 gccttaagcg gcaaaaccag cgaactgtct accgacgaca ttattcaggc catcacccca
      661 gcggtacggg aaaaatcgct ctctgccagc caaaaattat ggctggagtt acctggtaac
      721 cgcccacaac atgccgtcgg aacgccggtg ctgacactgg aaaatctgac tggcgaaggc
      781 ttcaggaatg tcagcctgac gctcaatgcc ggagaaattc tgggcctggc tgggctggtg
      841 ggagccggac gcacagaact ggccgagacg ctctatggtc tgcgtacttt gcgtggcgga
      901 cgcattatgc tgaatggtaa agagatcaat aaattatcca ctggagaacg tttactgcgc
      961 ggtctggttt atctgccgga agatcgccag tcatccggac tgaatctcga tgcttcgctg
     1021 gcctggaacg tctgcgccct tactcataac cttcgtggat tctgggcgaa aaccgcgaaa
     1081 gataatgcca ccctggaacg ttatcgtcgg gcgctgaata ttaaatttaa ccaaccggaa
     1141 caagctgcac ggacattatc cggtagcaac cagcaaaaaa tcctcattgc caaatgcctg
     1201 gaagcttcgc cgcaagcatt gattgtcgat gagccgacgc gcggcgtgga tgtctcggcc
     1261 cgtaatgata tctaccagct gttgcgcagc atcgccgcac agaatgtggc tgtgctgttt
     1321 atctcctctg atatggaaga gatcgaactg atggcagatc gtgtgtatgt gatgcatcag
     1381 ggcgaaatta cccactctgc actgaccggg cgcgatatta atgtcgagac tattatgcgc
     1441 gttgccttcg gcgatagtca gcgtcaggag gcatcatgct gaagtttatt cagaacaacc
     1501 gtgaaatcac ggcattgctg gcggtggtgc tgctgtttgt attacccggt tttctcgacc
     1561 gccagtattt aagtgtgcaa acgctgacca tggtttatag cagcgcgcaa atcctgatcc
     1621 tgctggcaat gggcgcgacg ctggtaatgc ttacgcgcaa tattgatgtt tcagtgggtt
     1681 cgattaccgg aatgtgcgcg gtgctgttgg ggatgttact gaacgcagga tattcactac
     1741 ctgttgcctg tgtcgcgact ttactgcttg gtttgctcgc gggatttttc aacggtgccc
     1801 tggtcgcgtg gctaaagatc cctgccattg ttgccaccct tggcacgtta gggttgtaca
     1861 gaggcatcat gttgctgtgg actggcggta aatggattga agggttaccc gccgaactga
     1921 aacagctctc cgccccgctg ctgcttggca tttcagcaat tggttggttg acgataattc
     1981 tggtggcatt tatggcctgg ctgctggcga agacggcgtt tggacgcagt ttttatgcca
     2041 cgggcgataa tttacagggc gctcgtcaac tgggcgttcg tacggaagcc attcgcattg
     2101 tggcattttc gttgaacggc tgcatggcgg cactggcggg aattgtgttt gcttcgcaga
     2161 ttggttttat cctcaaccag accggtaccg ggctggagat gaaagcaatt gcagcctgcg
     2221 tgctgggcgg cattagtttg ctcggtggtt ccggtgcgat catcggtgcg gtactcggcg
     2281 catggttcct gacgcagatc gatagcgtac tggtgctgtt gcgcattccg gcatggtgga
     2341 atgattttat cgcgggtctg gttctgctgg cggtgctggt gtttgatgga cgcctgcgtt
     2401 gtgcgctgga acgtaatcta cggcggcaaa aatatgcccg ctttatgacg ccaccgccat
     2461 ccgttaaacc cgcttcgtca ggtaaaaaac gggaggccgc ataatgcgta ttcgctacgg
     2521 ttgggaactg gctcttgccg cactgctcgt tattgagatt gtcgcatttg gtgcaattaa
     2581 cccgcgaatg ttagatctca atatgttgct gttcagcacc agtgacttta tctgcattgg
     2641 cattgtcgct ctaccgctga cgatggtgat tgtcagtggc gggatcgata tttcgtttgg
     2701 ttcgaccatc ggcctctgcg ccattgcatt gggcgtactg tttcaaagtg gtgtgccgat
     2761 gccgctggcg atactcctga ccttactgct cggcgcattg tgcgggctga tcaacgccgg
     2821 attaattatc tataccaaag ttaacccgct ggtgattacg cttggcacgc tgtatctgtt
     2881 tgccggaagc gctctgctgc tttccggtat ggccggagcg acggggtacg aaggtattgg
     2941 tggattcccg atggcgttta cagatttcgc taacctggat gtgctgggac tccccgttcc
     3001 gctgattatc ttcctgatat gtctcctcgt tttctggctc tggctgcata aaacccatgc
     3061 cggacgtaat gtgtttttga ttgggcaaag cccgcgcgtg gcgctttata gcgcgattcc
     3121 agttaaccgc accttatgtg cgctctatgc catgacgggg ctggcgtctg cggtcgccgc
     3181 tgtgctgctg gtatcgtatt ttggttcagc acgttccgat ctcggtgcgt cgtttctgat
     3241 gcccgccatc accgccgtgg tgcttggcgg ggccaatatt tatggtggtt ccggttccat
     3301 tatcggcacc gccattgcgg ttttattagt gggatatttg cagcaaggtt tgcaaatggc
     3361 aggagtgcca aatcaggtgt ccagcgccct ttccggtgcg ctgcttatcg tcgttgtcgt
     3421 aggtcgttcc gttagcctgc atcgccagca aattaaagag tggctggcgc gtcgggccaa
     3481 taacccattg ccataaagga tatcttcatg acacttcatc gctttaagaa aatcgcctta
     3541 cttagcgctc ttggcattgc cgcaatctct atgaatgtgc aggccgcaga gcgtattgca
     3601 tttattccca aactggttgg cgtgggattt tttaccagcg gtggcaacgg cgcacaacaa
     3661 gcgggtaaag agctgagcgt tgatgtgacc tacgacgggc cgacagaacc cagtgtttct
     3721 ggtcaggtac agttgattaa taacttcgtc aatcaaggtt ataacgccat tattcgtttc
     3781 tgcggtttcg cctgatggct tgtgtccggc actgaaacgc gccatgcaac gtggtgtgag
     3841 agtgctgacc tgggactctg atactaaacc ggagtgccgc tcttactaca ttaatcaggg
     3901 aacgcccgcc cagttgggag gtatgttggt ggatatggcg gcgcgtcagg tgaataaaga
     3961 caaagccaaa gtcgcgtttt tctactcaag ccccaccgtt acggaccaaa accagtgggt
     4021 gaaagaagcg aaagcgaaaa tcgccaaaga gcatccaggc tgggaaattg tcactacgca
     4081 gtttggctat aacgatgcca ctaaatcgtt acaaaccgca gaaggaatat taaaagcgta
     4141 tagcgatctc gacgccatta tcgcccccga tgccaacgcc ctgcccgctg ccgcacaagc
     4201 cgcagaaaac ctgaaaaatg acaaagtagc gattgtcgga ttcagtacgc caaatgtgat
     4261 gcgcccgtat gtagagcgcg gcacggtgaa agaatttgga ctgtgggatg tggttcagca
     4321 aggcaaaatt tcagtgtatg tcgcggatgt attattgaaa aaaggatcaa tgaaaacggg
     4381 cgacaagctg gatatccagg gcgtaggtca ggttgaagtc tcgccaaaca gcgttcaggg
     4441 ctatgactac gaagcggatg gtaatggcat cgtactgtta ccggagcgcg tgatattcaa
     4501 caaagagaat atcggcaaat acgatttctg atgtgcatta cttaaccgga gtaagttatg
     4561 gcagatttag acgatattaa agatggtaaa gattttcgta ccgatcaacc gcaacaaaat
     4621 atccctttta ccctgaaagg ttgcggtgcg c
//
LOCUS       DAZXUL010000221         4602 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZXUL010000221 DAZXUL010000000
VERSION     DAZXUL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.222"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="ACH3L1_004217"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="ACH3L1_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFT4078700.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="ACH3L1_004218"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="ACH3L1_004218"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFT4078701.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="ACH3L1_004219"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="ACH3L1_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFT4078702.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4602)
                     /gene="efeU"
                     /locus_tag="ACH3L1_004220"
     CDS             complement(3929..>4602)
                     /gene="efeU"
                     /locus_tag="ACH3L1_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFT4078703.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1116 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAZXUL010000222         4544 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZXUL010000222 DAZXUL010000000
VERSION     DAZXUL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.223"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            259..1116
                     /gene="focA"
                     /locus_tag="ACH3L1_004221"
     CDS             259..1116
                     /gene="focA"
                     /locus_tag="ACH3L1_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFT4078704.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1171..3453
                     /gene="pflB"
                     /locus_tag="ACH3L1_004222"
     CDS             1171..3453
                     /gene="pflB"
                     /locus_tag="ACH3L1_004222"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFT4078705.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3645..4385
                     /gene="pflA"
                     /locus_tag="ACH3L1_004223"
     CDS             3645..4385
                     /gene="pflA"
                     /locus_tag="ACH3L1_004223"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFT4078706.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4467..>4544)
                     /locus_tag="ACH3L1_004224"
     CDS             complement(4467..>4544)
                     /locus_tag="ACH3L1_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078707.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT         1157 a         1117 c         1096 g         1174 t
ORIGIN      
        1 acgatcgata ttgttacttt attcgcctga tgctcccttt taattaactg ttttagcgga
       61 ggatgcggaa aaaattcaac tcatttgtta atttttaaaa tttattttta tttggataat
      121 caaatagtta ctccgtattt gcataaaaac catgcgagtt acgggcctat aagccaggcg
      181 agatatgatc tatatcaatt tctcatctat aatgctttgt tagtatctcg tcgccgactt
      241 aataaagaga gagttagtgt gaaagctgac aacccttttg atcttttact tcctgctgca
      301 atggccaaag tggccgaaga ggcgggtgtc tataaagcaa cgaaacatcc gcttaagact
      361 ttctatctgg cgattaccgc cggtgttttc atctcaatcg cattcgtctt ctatatcaca
      421 gcaaccactg gcacaggcac aatgcccttc ggcatggcaa aactggttgg cggcatttgc
      481 ttctctctgg ggctgattct ttgtgttgtc tgcggagccg atctctttac ttccacagtg
      541 ttgattgttg ttgctaaggc gagtgggcgc atcacctggt gtcagttggc taaaaactgg
      601 ctaaatgtct attttggcaa cctggtcggc gcactgctgt ttgtactttt aatgtggctt
      661 tccggcgagt atatgaccgc aaatggtcaa tggggactaa acgtcctaca aaccgccgac
      721 cacaaagtgc accatacttt tattgaggcc gtctgtcttg gtatcctggc aaacctgatg
      781 gtatgtctgg cagtatggat gagttattct ggccgcagcc tgatggacaa agcgttcatt
      841 atggtgctgc cggtcgcgat gtttgttgcc agcggttttg agcacagtat cgcaaacatg
      901 tttatgatcc cgatgggtat tgtaatccgc gacttcgcat ctccggaatt ctggaccgct
      961 gtcggttctg caccggaaaa tttttctcac ctgaccgtga tgaacttcat cactgataac
     1021 ctgatcccgg ttacgatcgg taatattatc ggcggtggtt tgttggttgg gttgacatac
     1081 tgggtcattt acctgcgtga aaacgatcac cattaatggt tgtcgaagta cgcagtaaat
     1141 aaaaaatcca cttaagaagg taggtgttac atgtccgagc ttaatgaaaa gttagccaca
     1201 gcctgggaag gttttaccaa aggtgactgg cagaatgaag taaacgtccg tgacttcatt
     1261 cagaaaaact acactccgta cgagggtgac gagtccttcc tggctggcgc tactgaagcg
     1321 accaccaccc tgtgggacaa agtaatggaa ggtgttaaac tggaaaaccg cactcacgcg
     1381 ccagttgatt ttgacaccgc tgttgcttcc accatcacct ctcacgacgc tggctacatc
     1441 aataaagcgt tggaaaaagt tgttggtctg cagactgaag ctccgctgaa acgtgctctt
     1501 atcccgttcg gtggtatcaa aatgatcgaa ggttcctgca aagcgtacaa ccgcgaactg
     1561 gacccgatga tcaaaaaaat cttcactgaa taccgtaaaa ctcacaacca gggcgtgttc
     1621 gacgtttaca ctccggacat cctgcgttgc cgtaaatccg gtgttctgac cggtctgcca
     1681 gatgcttatg gccgtggccg tatcatcggt gactaccgtc gcgttgcgct gtacggtatc
     1741 gactacctga tgaaagacaa atacgctcag ttcacctctc tacaggctga tctggaaaac
     1801 ggcgtaaacc tggaacagac tatccgtctg cgcgaagaaa tcgctgaaca gcaccgcgct
     1861 ctgggtcaga tgaaagaaat ggctgcgaaa tacggctacg acatctctgg tccggctacc
     1921 aacgctcagg aagctatcca gtggacttac ttcggctacc tggctgctgt taagtctcag
     1981 aacggtgctg caatgtcctt cggtcgtacc tccaccttcc tggatgtgta catcgaacgt
     2041 gacctgaaag ctggcaagat caccgaacaa gaagcgcagg aaatggttga ccacctggtc
     2101 atgaaactgc gtatggttcg cttcctgcgt actccggaat acgatgaact gttctctggc
     2161 gacccaatct gggcaaccga atctatcggt ggtatgggcc tcgatggtcg taccctggtt
     2221 accaaaaaca gcttccgttt cctgaacacc ctgtacacta tggggccgtc tccggaaccg
     2281 aacatgacca ttctgtggtc tgaaaaactg ccgctgaact tcaagaaatt cgccgctaaa
     2341 gtgtccatcg acacctcttc tctgcagtat gagaacgatg acctgatgcg tccggacttc
     2401 aacaacgatg actacgctat cgcttgctgc gtaagcccga tgatcgttgg taaacaaatg
     2461 cagttcttcg gtgcgcgtgc aaacctggcg aaaaccatgc tgtacgcaat caacggcggc
     2521 gttgacgaaa aactgaaaat gcaggttggt ccgaagtctg aaccgatcaa aggcgatctc
     2581 ctgagctacg atgaagtgat ggagcgcatg gatcacttca tggactggct ggctaaacag
     2641 tacatcactg cactgaacat catccactac atgcacgaca agtacagcta cgaagcctct
     2701 ctgatggcgc tgcacgaccg tgacgttatc cgcaccatgg cgtgtggtat cgctggtctg
     2761 tccgttgctg ctgactccct gtctgcaatc aaatatgcga aagttaaacc gattcgtgac
     2821 gaagacggtc tggctatcga cttcgaaatc gaaggcgaat acccgcagtt tggtaacaat
     2881 gatccgcgtg tagatgacct ggctgttgac ctggtagaac gtttcatgaa gaaaattcag
     2941 aaactgcaca cctaccgtga cgctatcccg actcagtctg ttctgaccat cacttctaac
     3001 gttgtgtatg gtaagaaaac tggtaacacc ccagacggtc gtcgtgctgg cgcgccgttc
     3061 ggaccgggtg ctaacccgat gcacggtcgt gaccagaaag gtgctgtagc gtctctgact
     3121 tccgttgcta aactgccgtt tgcttacgct aaagatggta tctcctacac cttctctatc
     3181 gttccgaacg cactgggtaa agacgacgaa gttcgtaaga ccaacctggc tggtctgatg
     3241 gatggttact tccaccacga agcatccatc gaaggtggtc agcacctgaa cgttaacgtg
     3301 atgaaccgtg aaatgctgct cgacgcgatg gaaaacccgg aaaaatatcc gcagctgacc
     3361 atccgtgtat ctggctacgc agtacgtttc aactcgctga ctaaagaaca gcagcaggac
     3421 gttattactc gtaccttcac tcaatctatg taattagatt tgactgaaat cgtacagtaa
     3481 aaagcgtaca ataaaggctc cacgaaagtg gggccttttt tagcacgaga gccttttttg
     3541 tcagctatct atactttaag gtgactgcca aaacagactc gacgtagcct tcgagctgcg
     3601 caccaacacg gcctcagatg ggccacatct ggagaaacac cgcaatgtca gttattggtc
     3661 gcattcactc ctttgaatcc tgtggaaccg tagacggccc aggtattcgc tttatcacct
     3721 ttttccaggg ctgcctgatg cgctgcctgt attgtcataa ccgcgacacc tgggacacgc
     3781 atggcggtaa agaagttacc gttgaagatt tgatgaagga agtggtgacc tatcgccact
     3841 ttatgaacgc ttccggcggc ggcgttaccg catccggcgg tgaagcaatc ctgcaagctg
     3901 agtttgttcg tgactggttc cgcgcctgta aaaaagaagg cattcatacc tgtctggata
     3961 ccaacggttt tgttcgtcgt tacgatccgg tgattgatga actgctggaa gtaaccgacc
     4021 tggtaatgct cgatctcaaa cagatgaacg acgagatcca ccaaaatctg gttggagttt
     4081 ccaaccaccg cacgctggag ttcgctaaat atctggcgaa caaaaatgtg aaggtgtgga
     4141 tccgctacgt tgttgtccca ggctggtctg acgatgacga ttcggcacat cgcctcggtg
     4201 aatttacccg tgatatgggt aacgttgaga aaatcgaact tctcccctac cacgaactgg
     4261 gcaaacacaa atgggtggcg atgggtgaag aatacaaact cgatggtgtt aaaccaccga
     4321 agaaagagac catggaacgc gtgaaaggca ttcttgagca gtacggtcat aaggtaatgt
     4381 tctaatttcc tcgtaaagcg gcaactttat tgagttgccg cttttttatt ccgcgcttca
     4441 ttctgctaca gcgtttttaa cacatcttat agtgcatcta ccatatcccg tacctgagat
     4501 aataacgact gataatagct cgcgtgaagt tcttccccat cgaa
//
LOCUS       DAZXUL010000223         4530 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZXUL010000223 DAZXUL010000000
VERSION     DAZXUL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.224"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1222
                     /locus_tag="ACH3L1_004225"
     CDS             <1..1222
                     /locus_tag="ACH3L1_004225"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFT4078708.1"
                     /translation="PYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLYP
                     LSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISN
                     YAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVT
                     NIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKW
                     FIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPV
                     IVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLL
                     IANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAA
                     RVFVNDERFTRGGN"
     gene            1222..1956
                     /locus_tag="ACH3L1_004226"
     CDS             1222..1956
                     /locus_tag="ACH3L1_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4078709.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2423..3184
                     /locus_tag="ACH3L1_004227"
     CDS             2423..3184
                     /locus_tag="ACH3L1_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFT4078710.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3258..4530
                     /locus_tag="ACH3L1_004228"
                     /pseudo
     CDS             3258..4530
                     /locus_tag="ACH3L1_004228"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1243 a          862 c         1117 g         1308 t
ORIGIN      
        1 tccgtatggc attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg
       61 cgtagcgttg tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt
      121 gtcgttatac cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg
      181 tttattttgc gtcatcgtgc tggccgctct cgcggacaga ccccgctggc gcggacgtcc
      241 ccttagcggt ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat
      301 ctcaaactat gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga
      361 ccagcgtata cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg
      421 ttgggggtta ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac
      481 aaacatagag agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga
      541 aggtggcatc gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtggccgtg
      601 tatatgccgc ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat
      661 tgccttaatg gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc
      721 tgttccccat ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat
      781 gaatcaggta cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat
      841 tatgaccgcc gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag
      901 agctgaagct gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata
      961 cctgacgcag gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg
     1021 gttcgttcgc gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc
     1081 aatccacaat gatgcagacg taagcgctgc aatgatattc attgcgcaca ggcgcggtga
     1141 ttatctgaca gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg
     1201 ttttactcgc gggggaaact aatggttaac gatctgacgt ataagaaatt agaaagcaca
     1261 aacatcaact gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt
     1321 ggcctggatc tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg
     1381 aaacttcagg ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc
     1441 gtgatatggg taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag
     1501 gtttgtcgtc gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga
     1561 atgacgaatg atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg
     1621 gatattttat tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg
     1681 tttataaaac gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt
     1741 agcacacgta aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag
     1801 tccgcaattc gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt
     1861 aatgctcaac ttatacaaca ggcgataccc gcgttgggaa gagatgcgtc aaggtatgaa
     1921 agatgtaaca atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct
     1981 ttttcgatga cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt
     2041 tttgccacgg gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag
     2101 agagttaata aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg
     2161 ttttttaaat tttcaggttt tggttaaata tttgcttgca aatataggtg attttttatt
     2221 gtaggctttt gaaggctatt ataactgtgc gctaaattaa tgcgttgaat aaaagaaata
     2281 acaaaattaa gaaaagccat atttacgata tgggcaacat aaatataatc taaaaaagag
     2341 taaggcgttg taggttggtt ccgcgttcat taccttgtta gtgaacgcgg atttatcagt
     2401 tgtcgaaatg gtttttataa taatgaaaaa ttttaatgta ctaaccaaag tcgtattttg
     2461 cttgtcgttt ttatcaagcg catttcccac ctatgctgcg gatgatgtca aagtgactca
     2521 aaacacttca gtttttaaac tgaagcttag tgcaacgcga ttaatctatg aacaagggtc
     2581 ggatggcgca gcgataactg tgacaaatcc gcaggattgg ccgatgcttg tgcagagtaa
     2641 agtctttgaa gaagataaag taactgcagc gccctttatt gtcactccgc cactgttcag
     2701 gcttaatcca ggccagcaaa ataaaatgtg ggttgtgcag actgcgccag ctttacaaga
     2761 cagggaaaaa ttacactggc tatgcgccac cggcattacg cctaaaaaga gtgatgtttg
     2821 ggccgagaaa gataaagaaa aaattgctga aaatgtgaac ctgaacatcc aggttaacgt
     2881 acatacctgc ataaaactta ttaccaggcc atctggtatt gaaggcaaga tggttaatgc
     2941 gagtgagaag ttatcgtgga aaaagcaggg tgataaaatt gtcgcggtca atcagtcgcc
     3001 gttctacatc aacctttctt ctttacaggt tggcggtagc aagattgaga aactagatta
     3061 tattgcgccg ttttcgcaac atgagtttgt tatgccaaaa actagcggcc aggatattaa
     3121 atggtctgtg attactgatg aaggtggtga gagtcgtttg tttatatctc atttgcaatc
     3181 ataatgtatt acatttttat tgaatatttt tatgccatgc atattccatg ttttatcgac
     3241 tgtcattaat tttgataatg gccttacttg cgggtcagtt aagtgcccag gagtggtcat
     3301 ttgattcttc acagttggaa ggaaacgtta gtgcggacac tgtggcaatg tttaatcagg
     3361 gagagcaact ccccggaaat tatcgtgtgg aaatttatct taacggtgaa aaagttgatg
     3421 ttggggagtt tccctttcat cgtccggaat cccctgaaga aaaagagctt gttccatgtt
     3481 taaccgttga tgaccttatc cactatggca ttaaaattga taaaagcagc agtgatactg
     3541 acaataaaaa gaatcagtgt tttaaatgga actctattga aggtttgaaa gtaaattatg
     3601 attttgatag ccagcgcgta cagataaccg ttccacaact gtatctgcaa gataaaaaaa
     3661 gtagtttagc accagtgtcg ttatggaatg agggtgtagc tgcattccgc atggtgtatc
     3721 agacaaatat tgatatttcc aaacaaaatg ataatcaatc taccacgcgt aattctcgtt
     3781 atggcaggtt cactccaggg tttaatcttg gtgcctggag gtttcgtagt tcagtgacat
     3841 ggagcaagga acttggccag tcagaaagat ggcaaagggg ttacatgtgg tttgagcgag
     3901 gcataaatgc gattaaaagc cgattaacgc ttggagaatc ttatacgtcg tctgaggtgt
     3961 ttgacagcat accttttcgc ggcgggatgt tagcgaccga cgatgcgatg actccgccgg
     4021 aggacagcta ctacacacct gtggtgcatg gaatagcaca aagtgaggcg caggttatta
     4081 ttaaacaaaa tgggcaaatt atttttaccc gttctgtgcc gccagggcct tttgcgctgg
     4141 ataatttacc aacgctggcc gttggcgggg agcttgatgt tacagtgaga gagagtaatg
     4201 gcgaggagca gtatttttcc gtgccatttc aaacgccggc catcgctctg catgaaggat
     4261 atttcaaata tagtgtgatg ggggggaata taaagaagaa ggtataaaaa gctcccctgt
     4321 ctccgtgggc gaaatcacgg cgatgtacgg gcttccttat ggcgtcacgg tgtatggagg
     4381 gattcaaaac gccactcatt ttaatgcaat atctacgggt attggcattt ctctggggct
     4441 gttagggagt ttatcgacag atattacgcg ttcgattgcg aatttatatt atgggaataa
     4501 atatcgtatt cgttatagta aatccattag
//
LOCUS       DAZXUL010000224         4348 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZXUL010000224 DAZXUL010000000
VERSION     DAZXUL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.225"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..116
                     /locus_tag="ACH3L1_004229"
     CDS             <1..116
                     /locus_tag="ACH3L1_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078711.1"
                     /translation="TISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(113..379)
                     /locus_tag="ACH3L1_004230"
     CDS             complement(113..379)
                     /locus_tag="ACH3L1_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFT4078712.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="ACH3L1_004231"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="ACH3L1_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFT4078713.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            complement(4133..>4348)
                     /locus_tag="ACH3L1_004232"
     CDS             complement(4133..>4348)
                     /locus_tag="ACH3L1_004232"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078714.1"
                     /translation="TFNFLYVILRGLYAFIVTDFIFCWAIALPLIFQHIKRQARTVVR
                     AFFLELDNPYPEHLIGVAFFRFSGHLC"
BASE COUNT         1062 a         1139 c         1130 g         1017 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaacttctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagt
//
LOCUS       DAZXUL010000225         4209 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZXUL010000225 DAZXUL010000000
VERSION     DAZXUL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.226"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..209)
                     /locus_tag="ACH3L1_004233"
     CDS             complement(<1..209)
                     /locus_tag="ACH3L1_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFT4078715.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACH3L1_004234"
     tRNA            complement(324..399)
                     /locus_tag="ACH3L1_004234"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACH3L1_004235"
     tRNA            complement(406..480)
                     /locus_tag="ACH3L1_004235"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACH3L1_004236"
     tRNA            complement(597..681)
                     /locus_tag="ACH3L1_004236"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACH3L1_004237"
     tRNA            complement(690..765)
                     /locus_tag="ACH3L1_004237"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACH3L1_004238"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACH3L1_004238"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFT4078716.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACH3L1_004239"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACH3L1_004239"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFT4078717.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACH3L1_004240"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACH3L1_004240"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFT4078718.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAZXUL010000226         4198 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZXUL010000226 DAZXUL010000000
VERSION     DAZXUL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.227"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            84..752
                     /gene="ykgH"
                     /locus_tag="ACH3L1_004241"
     CDS             84..752
                     /gene="ykgH"
                     /locus_tag="ACH3L1_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFT4078719.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="ACH3L1_004242"
     CDS             complement(995..1690)
                     /locus_tag="ACH3L1_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFT4078720.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="ACH3L1_004243"
     CDS             complement(1683..3110)
                     /locus_tag="ACH3L1_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFT4078721.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="ACH3L1_004244"
     CDS             complement(3121..3840)
                     /locus_tag="ACH3L1_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFT4078722.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAZXUL010000227         4188 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZXUL010000227 DAZXUL010000000
VERSION     DAZXUL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.228"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..192
                     /locus_tag="ACH3L1_004245"
     CDS             <1..192
                     /locus_tag="ACH3L1_004245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078723.1"
                     /translation="SIQSNRDWLSMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGIT
                     NIISIILQQAQDYDVAKIT"
     gene            complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACH3L1_004246"
     CDS             complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACH3L1_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFT4078724.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACH3L1_004247"
     CDS             complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACH3L1_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFT4078725.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1474..4149
                     /gene="adhE"
                     /locus_tag="ACH3L1_004248"
                     /gene_synonym="adhC"
     CDS             1474..4149
                     /gene="adhE"
                     /locus_tag="ACH3L1_004248"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFT4078726.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1022 c          962 g         1036 t
ORIGIN      
        1 tcaattcaat caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt
       61 agtgaaatgc acaataattt aacaattgat tattatttag cactttcctc taccaaaggc
      121 agtggaatta ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg
      181 aaaataacat gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg
      241 agaagaatat ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa
      301 taatgacgaa gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca
      361 ggccggggaa aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca
      421 atagccccat aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc
      481 acggtgccat gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga
      541 aaccaaacag atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg
      601 cccctggccc cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg
      661 cggtttctga tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg
      721 acatcgctat cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac
      781 caaaaagttg tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag
      841 acaggttggc tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc
      901 tgataaagac gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt
      961 tgaagtaaac gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg
     1021 gataatgtta gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca
     1081 tttttcacct cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa
     1141 aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg
     1201 aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta
     1261 cccagaagtg agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc
     1321 taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt
     1381 tcatcggtta agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt
     1441 actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt
     1501 aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag
     1561 caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc
     1621 gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac
     1681 cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg
     1741 tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt
     1801 atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag
     1861 acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg
     1921 gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg
     1981 atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg
     2041 atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct
     2101 atcggtgtag gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt
     2161 gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa
     2221 cagtctgttg ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac
     2281 ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat
     2341 ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc
     2401 ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc
     2461 gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc
     2521 gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct
     2581 tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg
     2641 aaaacagctc gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat
     2701 aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc
     2761 tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa
     2821 aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc
     2881 gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc
     2941 ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa
     3001 actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca
     3061 gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg
     3121 gacgccgcga agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg
     3181 gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa
     3241 gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg
     3301 gttgtaactg acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg
     3361 gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc
     3421 ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag
     3481 ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc
     3541 taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc
     3601 gcgggtatcg catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg
     3661 ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt
     3721 cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg
     3781 caggctcgcc gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac
     3841 cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg
     3901 ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg
     3961 gataaactgt ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg
     4021 ctgatctccg agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa
     4081 gatgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa
     4141 tccgcttaat cagtagcgct gtctggcaat ataaacggcc ccttctgg
//
LOCUS       DAZXUL010000228         4182 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZXUL010000228 DAZXUL010000000
VERSION     DAZXUL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.229"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..186)
                     /locus_tag="ACH3L1_004249"
     CDS             complement(<1..186)
                     /locus_tag="ACH3L1_004249"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFT4078727.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACH3L1_004250"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACH3L1_004250"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFT4078728.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="ACH3L1_004251"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="ACH3L1_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFT4078729.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="ACH3L1_004252"
     CDS             3863..4144
                     /locus_tag="ACH3L1_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFT4078730.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1043 c         1062 g         1061 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAZXUL010000229         4168 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZXUL010000229 DAZXUL010000000
VERSION     DAZXUL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.230"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACH3L1_004253"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACH3L1_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFT4078731.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACH3L1_004254"
                     /pseudo
     CDS             complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACH3L1_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACH3L1_004255"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACH3L1_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            3787..>4168
                     /locus_tag="ACH3L1_004256"
     CDS             3787..>4168
                     /locus_tag="ACH3L1_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFT4078732.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1021 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAZXUL010000230         4114 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZXUL010000230 DAZXUL010000000
VERSION     DAZXUL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.231"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..158)
                     /locus_tag="ACH3L1_004257"
     CDS             complement(<1..158)
                     /locus_tag="ACH3L1_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFT4078733.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACH3L1_004258"
     CDS             complement(308..469)
                     /locus_tag="ACH3L1_004258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078734.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACH3L1_004259"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACH3L1_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            914..1249
                     /locus_tag="ACH3L1_004260"
     CDS             914..1249
                     /locus_tag="ACH3L1_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFT4078735.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="ACH3L1_004261"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="ACH3L1_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="ACH3L1_004262"
     tRNA            complement(2161..2237)
                     /locus_tag="ACH3L1_004262"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="ACH3L1_004263"
     tRNA            complement(2339..2414)
                     /locus_tag="ACH3L1_004263"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="ACH3L1_004264"
     CDS             complement(2559..3380)
                     /locus_tag="ACH3L1_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFT4078736.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="ACH3L1_004265"
     CDS             complement(3395..3751)
                     /locus_tag="ACH3L1_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFT4078737.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="ACH3L1_004266"
     CDS             complement(3764..>4114)
                     /locus_tag="ACH3L1_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFT4078738.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1118 c          793 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAZXUL010000231         4100 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZXUL010000231 DAZXUL010000000
VERSION     DAZXUL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.232"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACH3L1_004267"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACH3L1_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFT4078739.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="ACH3L1_004268"
     CDS             complement(1433..1759)
                     /locus_tag="ACH3L1_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFT4078740.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="ACH3L1_004269"
     CDS             1894..2235
                     /locus_tag="ACH3L1_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFT4078741.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="ACH3L1_004270"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="ACH3L1_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="ACH3L1_004271"
     CDS             complement(2833..3543)
                     /locus_tag="ACH3L1_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFT4078742.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="ACH3L1_004272"
     CDS             3651..3956
                     /locus_tag="ACH3L1_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFT4078743.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1116 a         1035 c          911 g         1038 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgaact actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg
//
LOCUS       DAZXUL010000232         4054 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZXUL010000232 DAZXUL010000000
VERSION     DAZXUL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.233"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACH3L1_004273"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACH3L1_004273"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFT4078744.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACH3L1_004274"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACH3L1_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFT4078745.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACH3L1_004275"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACH3L1_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFT4078746.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
BASE COUNT         1080 a         1060 c          888 g         1026 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gata
//
LOCUS       DAZXUL010000233         4042 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZXUL010000233 DAZXUL010000000
VERSION     DAZXUL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.234"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(126..284)
                     /locus_tag="ACH3L1_004276"
     CDS             complement(126..284)
                     /locus_tag="ACH3L1_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFT4078747.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
     gene            complement(886..1077)
                     /locus_tag="ACH3L1_004277"
     CDS             complement(886..1077)
                     /locus_tag="ACH3L1_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFT4078748.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="ACH3L1_004278"
     CDS             1142..1465
                     /locus_tag="ACH3L1_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFT4078749.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1674..2105
                     /locus_tag="ACH3L1_004279"
                     /pseudo
     CDS             1674..2105
                     /locus_tag="ACH3L1_004279"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="ACH3L1_004280"
     CDS             2143..2673
                     /locus_tag="ACH3L1_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFT4078750.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="ACH3L1_004281"
     CDS             2680..2913
                     /locus_tag="ACH3L1_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFT4078751.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="ACH3L1_004282"
     CDS             complement(3459..3851)
                     /locus_tag="ACH3L1_004282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078752.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAZXUL010000234         3966 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZXUL010000234 DAZXUL010000000
VERSION     DAZXUL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.235"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..224
                     /locus_tag="ACH3L1_004283"
     CDS             <1..224
                     /locus_tag="ACH3L1_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFT4078753.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="ACH3L1_004284"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="ACH3L1_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFT4078754.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="ACH3L1_004285"
     CDS             518..1108
                     /locus_tag="ACH3L1_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFT4078755.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /locus_tag="ACH3L1_004286"
     CDS             1348..1608
                     /locus_tag="ACH3L1_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFT4078756.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="ACH3L1_004287"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="ACH3L1_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFT4078757.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="ACH3L1_004288"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="ACH3L1_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFT4078758.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="ACH3L1_004289"
     CDS             3457..3648
                     /locus_tag="ACH3L1_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078759.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
     gene            complement(3878..>3966)
                     /locus_tag="ACH3L1_004290"
     CDS             complement(3878..>3966)
                     /locus_tag="ACH3L1_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078760.1"
                     /translation="RFTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT         1074 a          984 c          986 g          922 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttaattac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga gcgataggaa ttaaaacccc aaaaagatta
     3721 aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctccatat gaacggaacc
     3781 gacgattaaa ctggatggct ctgattgatt cagggtatga atggcggttt tttgctccgt
     3841 ttccctcaaa atggacgcaa cttcccctct gcggctctca gccgcaccac cgcatccggg
     3901 ccagcggctc atgcatcagg acctgctctg ccagacggta gccccgcttc agccccgtaa
     3961 aacgca
//
LOCUS       DAZXUL010000235         3897 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZXUL010000235 DAZXUL010000000
VERSION     DAZXUL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.236"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..141)
                     /locus_tag="ACH3L1_004291"
     CDS             complement(<1..141)
                     /locus_tag="ACH3L1_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFT4078761.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETL"
     gene            179..648
                     /locus_tag="ACH3L1_004292"
                     /pseudo
     CDS             179..648
                     /locus_tag="ACH3L1_004292"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            681..1081
                     /locus_tag="ACH3L1_004293"
                     /pseudo
     CDS             681..1081
                     /locus_tag="ACH3L1_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1665..2381
                     /locus_tag="ACH3L1_004294"
     CDS             1665..2381
                     /locus_tag="ACH3L1_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078762.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2615..2743
                     /locus_tag="ACH3L1_004295"
                     /pseudo
     CDS             2615..2743
                     /locus_tag="ACH3L1_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2933..3337
                     /locus_tag="ACH3L1_004296"
     CDS             2933..3337
                     /locus_tag="ACH3L1_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078763.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            3400..3897
                     /locus_tag="ACH3L1_004297"
                     /pseudo
     CDS             3400..3897
                     /locus_tag="ACH3L1_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1089 a          821 c          931 g         1056 t
ORIGIN      
        1 cagagtctcc ggcgtacagc caatctttgg agcaatggaa caaattgtcg cccattgtga
       61 gtcatattcg ccctgacttt ccagaaccat acggactgcc cgttgacgga cttcggggga
      121 aaaacgagta tttttagtca tcctgtttac ctctttctca ggaagtttag tctccaggat
      181 tcccggggcg gttcagaatg aacatccgca tcttcctgtt gaaagtcgtc gtcaggcaaa
      241 gggaactcgc ttcctgtggc agcacagcga taaatggatg accgaatcca ggcaggaaaa
      301 gctgatatgg ctgcgtgcac agatgaagct gacgagcctg tgctgggcgc tgaaagaact
      361 ggcaaaggat atctggagca gaccatggag cgaggaaaga cgcaacgact ggcagagatg
      421 gctgcggcca acagtgacgt ccccatgatg aagaatgtgg ctaaaacgat agggaaaagg
      481 ttgtacggta tcctgaacgc catgcgccat ggcgtatcaa acggaaatgc ggaagcactg
      541 aacagcaaga tcaggctgct gagaataaaa gccaagggct accgcaaccg ggagcgcttt
      601 aagctgggag tgatgttcca ctatgggaaa ctgaatatag cgttctaagc cttcccacca
      661 tgatcgggga agccccagat atgaacaggc acattcagag ttactttaaa gagcttactg
      721 aacgtctgag gtgttagagc catgactgcc gcagcattgc tggcggaaat gcctgaatcg
      781 agcagcctct cgagacgtga aatcagcgca cttgtcggtg ttgctcaggt aaaccgggac
      841 tcgggaactc tgcgcggtcg acgaaccatt ttcggtgatt gtgctgggga agaacagctc
      901 tgtacatggc ggcgcttgag gccccccccg gtttaatctg gtgataaagg cgttttacat
      961 gcgcctactt gcagccggaa acgccaaaaa agtggcactg gttgcctgta tgcgtaagtt
     1021 gctgaccatt atgaacgcaa tgctcagaaa aaacgaagaa tggaatgaat cgtatttatg
     1081 aaaactggat tgtgggccat aaatattcca aaaagatagc ccgctccgtt tattaccgga
     1141 acgggcgaga gcatgacatt gtctgttatt ataatgagac tgctgattct acttattttt
     1201 aatttcaaga aaagcgtttt ctacaaagaa ttatgagatt taatgttttg tttagacaat
     1261 attatcattc tgttcaataa tgaaagtctt catcaacaac aagtaaaaac aacgtccctc
     1321 tcatcgaacc ggacttgcag atttcccgca tccggctctc ttgcaacaca tcagaccttc
     1381 acgttcgggc ggttatgggg aaggtccagc aagctgtatg agccctaact gtttgaatac
     1441 cagtagatta ccagaaactt ctatcgattg aagacattac caactagcgt tactaaatgt
     1501 tgaccaagtt ttgttcactt gtctggtttt gatagtagca ttctgcagag tattttgata
     1561 tgataaatat gcattaacaa tgatattatt gttaatttcc tagcatgaaa attaaaagat
     1621 caacttttat ctcgaatatt ttttacatta tcagttagtt tcttatgaat gacaattcat
     1681 tgcttagaaa ttcttcatta ttcatcgcct atatgggatg tgtagggtgg gtcagtgctt
     1741 attcttatgg ctggggaacc tctttttact atggatttcc atggtgggtt gttggtgcag
     1801 gtttggatga tgttgccaga agtttactct atgctatcat agtaatgggt atacttttta
     1861 ccggttgggg gattggtatt ttattttttc ttcttataaa gaaaagaagt aaaatacaag
     1921 acttgagttt ttttagacta ttttttgcta ttaccctact gtttttccct gtaatattcg
     1981 aacttttaat tctcaaacaa tatttcatac ttccattatc actttcattc attattagtt
     2041 cattggttat ttcaataata attcgtatat atggacggat cttttctgta tcatgttttt
     2101 cagatatccc ttttgttaga gaacaccgca ttaagttaat tatggctggt tttttggttt
     2161 atttttggtt tttttccttt cttgttggct ggtataaacc tcaattaaaa aaagaatatc
     2221 agatgctttg ttataataat agttggtatt atgttcttgc tcgttatgat tcacggctcg
     2281 ttttatctag ttcatttaaa gatgacagta acagattctt aatatttaat acagaacagt
     2341 cgggatttta tgaaatcaat gatgtttatg tcagaaagta ataatcctct cccacaaagt
     2401 gaattaaatg aatatataat gcgttcgaag tatatgtatt gagctgtcat actgagaaac
     2461 aaaccttagg gcagcacaat taaagccaga aacaactatg caagaaagtt actaaataag
     2521 tgctcggaac agtaaaacac tcagggggga ctcagtccgg attatgtaat cagataaatt
     2581 gccaaacatc acaaatcacc agccggactg agtgatgccg agcattgcac caataccccg
     2641 tgatgaacga tgcctgatgc agaaagctcc ctataaaaca aagaacaaaa actacgaccg
     2701 gcgattcacc gtcatactga tgctgcatcg tggtgacact gtctgtcact ttcatgttga
     2761 ctgaacaact ctgctgcgaa gaaggtgtac cgccggcggg atttgattgt ccggcgtcta
     2821 gcttccactt ccctgatgga actgaaagtt gttatggccg gtcagcttgc cctgtccgca
     2881 ggacatgagg tgctgtgacg gacagcacca gtaacggaaa gtgacaatga aaatgaatac
     2941 tgcgctgata gttgcactaa tgtgtatgtg gtatgcggta cccgctgctg caaaagagac
     3001 tttactggct atgccacgta attcaacaga acattgctat gcggagatta atgtgcacgg
     3061 accatatggg gtgtatttcc gagtggtccc gcatcccccc gggggaaaga gctgggtcga
     3121 atgtaacagt gactactatt actctgataa accaccggga gtacagatac tcggaacccg
     3181 tgcagggtgt cgggtatacg gaatctgcgg gaccacctcg acactgcatg tggctggaag
     3241 aggggttgtg tgtattaaaa atatatgcag tccacgtggg atgataattc acaggatacg
     3301 gaaacgtcct gtagtggctg taagcgatga gatgtgatct tacccagcaa tagtagacac
     3361 gcggctaagt gagtaaactc tcagtcagag gtgactcaca tgacaaaaac agtatcaacc
     3421 agcaaaaaaa cacgtaaaca gcattcgcct gaattttgca gtgaagccct gaagcttgct
     3481 gaacgcatcg gtgttgctgc cgcagcccgt gaactcagcc tgtatgaatc tcagctctat
     3541 gcgtggcgca gcaaactgca gcaacagatg acctcgtctg agcgtgaaag tgaactgccc
     3601 gcctgaagcg gcaactggcg gagcaggcag aggaactggc tatcctccaa aaggccgcga
     3661 catactttgc gcagcggctg gtatacgtgg tgtcagcgtc ggacaggaat aagccctcgt
     3721 cagcagttcc gtcagcactg cgacagcgtt gtcctcgcgg cggcctttac ccggtcaaaa
     3781 cagcgttacg gtgcaccacg cctgacggat gaactgcgtg ctcagggtta ccactttaac
     3841 gtaaaaaccg tggcggcaag cctgcgccgt caggggctga gggcaaaggc ctcccgg
//
LOCUS       DAZXUL010000236         3880 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZXUL010000236 DAZXUL010000000
VERSION     DAZXUL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.237"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            120..1454
                     /gene="adeQ"
                     /locus_tag="ACH3L1_004298"
                     /gene_synonym="yicO"
     CDS             120..1454
                     /gene="adeQ"
                     /locus_tag="ACH3L1_004298"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFT4078764.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1507..1959
                     /locus_tag="ACH3L1_004299"
     CDS             1507..1959
                     /locus_tag="ACH3L1_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFT4078765.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2170..3360
                     /gene="nepI"
                     /locus_tag="ACH3L1_004300"
     CDS             2170..3360
                     /gene="nepI"
                     /locus_tag="ACH3L1_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFT4078766.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3401..3694)
                     /gene="yicS"
                     /locus_tag="ACH3L1_004301"
     CDS             complement(3401..3694)
                     /gene="yicS"
                     /locus_tag="ACH3L1_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFT4078767.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
BASE COUNT          800 a          863 c         1025 g         1192 t
ORIGIN      
        1 gcgagattat taaagaatat ctattaatgt gcaattgaaa tgtgataatt atcactgaac
       61 cgttgttgtc atttgtttta caaaagcaag ggattaaatc tcctccaatg gacaaaaaaa
      121 tgaataatga caataccgat tacgtgagta atgaatcagg gacgctttcg cgattattta
      181 aactacctca gcatgggacc accgtccgca cagaattgat tgcggggatg accacttttt
      241 taaccatggt gtacattgtt tttgtgaacc cgcaaatcct cggcgcggca caaatggacc
      301 cgaaagtggt gtttgttacc acctgtttga ttgccggtat cggcagtatt gcgatgggga
      361 tatttgctaa cttacccgtg gcgctggctc cggcaatggg gctgaacgcc ttctttgcgt
      421 tcgtggtcgt gggggcgatg gggatctcct ggcagaccgg gatgggcgcg atattctggg
      481 gcgcaattgg gctatttttg ctcacgctgt ttcgtatccg gtactggatg atctccaaca
      541 ttccattaag tttacgtatt ggtatcacca gtggaatcgg attatttatt gccttaatgg
      601 gattaaaaaa cactggtgtt attgtcgcca ataaagacac gctggtgatg attggcgatt
      661 taagttctca cggcgtgttg ttaggtattt tagggttttt tattataacc gtgttgtcat
      721 cacgtcattt tcatgccgcg gtgctggttt ctattgtggt gacgtcttgc tgtggattat
      781 ttttcggtga tgttcatttt agcggcgtct attccattcc gcctgatatt agcggcgtca
      841 ttggtgaagt agatttgagc ggcgcgttaa cacttgaact cgccggtatc attttctcct
      901 ttatgctgat caacctattt gattcatcag gaacattaat tggtgtaact gataaagcgg
      961 gcttaataga tagcaacggt aaattcccca atatgaataa ggcgctgtat gttgatagcg
     1021 tcagttcggt ggcaggcgca tttatcggta cctcgtctgt tactgcctat attgaaagta
     1081 cttctggtgt ggcagtcggt ggtcgcacgg ggctaacggc tgtcgtggtc ggcgttatgt
     1141 tcctgttagt tatgttcttc tcaccgctgg tggcgatggt tcctccttac gcaaccgctg
     1201 gagcgttaat ctttgttggc gtgctgatga cttcgagcct ggcgcgcgtt aactgggatg
     1261 attttaccga atcggtgcct gcgtttatta ccacggtgat gatgcccttt actttctcga
     1321 tcaccgaagg gattgcactc ggctttatgt cgtactgcat catgaaagtg tgcaccgggc
     1381 gctggcgcga tctgaacctg tgtgtggtgg tggtcgcagc tctgttcgca ttgaagatta
     1441 ttctggtgga ttagtaactg gcgagggttt ccagatatca tgagttctga ttacgcagga
     1501 gaactcatga tctggataat gctcgccacg ctggcggtag tgtttgtggt tggttttcgg
     1561 gtgctgacat ccggggccag aaaagcgatt cgccgtctca gcgatcggct gaatatcgat
     1621 gtcgtacccg tggagtcgat ggtcgatcaa atgggaaagt cagccggtga cgaattttta
     1681 cgttatttgc atcgcccgga tgagtcgcac ctgcaaaacg ccgcgcaggt gttgcttatc
     1741 tggcaaattg tcattgtcga tgatagcgaa cagaacctgc tgcaatggca ccggatttta
     1801 caaaaagccc gcctttccgc gccgattacc gatgctcagg tcaggctggc gctgggtttt
     1861 ctgcgtgaaa ccgaacctga aatgcaggat attaatgctt ttcagatgcg ctataacgcg
     1921 ttctttcagc ctgccgaggg cgttcactgg ttgcattgaa accgtttcgt cttatcagcc
     1981 tacagaccgc gcctgacctg tcgggtggat aagacgttca cgccgcatcc ggtaatcttt
     2041 gccctgttcc gtttcgatct taactccagc tcaagcaaca ccgtaaaagt tgcaaaaacg
     2101 ttaaattcgt cacacatttc aatgttaaag tggtcggctt ttcccctgaa acatgccacg
     2161 ggtaacacca tgagtgaatt tattgccgaa aaccgcggcg cggatgccat cacccgaccg
     2221 aactggtcag ccgttttctc ggtggcgttt tgtgtcgcct gtctgattat cgttgagttt
     2281 ttgcccgtca gtttgttgac gccaatggcc caggatttag gcatttcgga aggggttgcc
     2341 gggcaatcgg tgaccgtgac cgcctttgtg gcaatgtttg ccagtttgtt tattacccag
     2401 acaattcagg ccactgaccg ccgctacgtt gttattttgt ttgccgtttt gctgacgctc
     2461 tcctgcttgc tggtttcctt tgctaactca ttcagtttgc ttttaatcgg tcgtgcctgt
     2521 ctggggctgg cgctgggcgg gttctgggcg atgtcggcgt cgctgaccat gcgtctggta
     2581 ccgccgcgta cggtgccgaa ggcgctgtcg gtgatcttcg gcgcggtttc tattgcgctg
     2641 gtgattgccg cgccgttggg cagtttttta ggcgagctta tcggttggcg caatgtcttt
     2701 aatgcggcgg cggtgatggg cgtgctgtgt attttctgga ttatcaaatc attgccttca
     2761 ctgccaggcg aaccctcgca tcagaaacaa aatactttcc gcttattaca acgtccgggt
     2821 gtgatggcag ggatgatcgc catcttcatg tctttcgccg ggcagtttgc tttcttcacg
     2881 tatattcgcc cggtgtatat gaacctggcg ggattcggcg tggatggctt aacgctggtg
     2941 ctgttgagtt ttggtatcgc cagctttatt ggtacgtcgc tttcgtcgtt cattcttaaa
     3001 cgttcggtaa aactggcctt agcaggcgcg ccgttaatac tggctgtgag tgcgttggta
     3061 ctgacgttgt ggggaagcga taaaatcgtt gctaccggcg tggcgattat ctgggggcta
     3121 acttttgcat tggttcccgt cggctggtca acgtggatca cccgctcgct ggccgatcag
     3181 gcagaaaaag ccgggtctat tcaggtggcg gttattcagc ttgctaatac ctgtggcgcg
     3241 gcaatcggcg gttatgcgct ggataatatt ggtctgactt cgccgttgat gttgtccggc
     3301 acattgatgt tgctgactgc attgttggtt actgcaaagg tgaaaatgaa gaaatcctga
     3361 tatcaggtaa agaagcgttg gtcagtagtt gttgctgttc ttacatatcc gggcattcta
     3421 ccttacccaa cgcttcccaa taattgctct gattattacg ttccatcgcc acgatggcct
     3481 cgcgtatatg ttgtttattg ttttcatctg acaacattaa cttttcccac gcttcatctg
     3541 ataatgacgc atgtggcgtg caaattttac gtaaatcttg taataccgtg gttttctctt
     3601 ttgcggactg cagtgagcta aaaggcgact cggcgtatac gatgcctggc atagcgaaaa
     3661 aagtgaaaat aagcagtagt gtcgttggtt tcatggattc aaccttttac aaatgtgtat
     3721 cgaatgaagc ataagtgtag tttgctttca tcgctctcat catgctgtac gaaacgcgct
     3781 tgcgtggtca gtaagaagtg ccagacttta tattccactt ttattccttt ttattcttat
     3841 cgatagcgtt tcgtttttta aaccgcagcg accttaccgc
//
LOCUS       DAZXUL010000237         3824 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZXUL010000237 DAZXUL010000000
VERSION     DAZXUL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.238"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            59..334
                     /gene="ypdI"
                     /locus_tag="ACH3L1_004302"
     CDS             59..334
                     /gene="ypdI"
                     /locus_tag="ACH3L1_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFT4078768.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(411..653)
                     /locus_tag="ACH3L1_004303"
     CDS             complement(411..653)
                     /locus_tag="ACH3L1_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFT4078769.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            1006..1926
                     /gene="lpxP"
                     /locus_tag="ACH3L1_004304"
     CDS             1006..1926
                     /gene="lpxP"
                     /locus_tag="ACH3L1_004304"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFT4078770.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2281..2352
                     /gene="ypdK"
                     /locus_tag="ACH3L1_004305"
     CDS             2281..2352
                     /gene="ypdK"
                     /locus_tag="ACH3L1_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFT4078771.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2417..3655)
                     /gene="alaC"
                     /locus_tag="ACH3L1_004306"
     CDS             complement(2417..3655)
                     /gene="alaC"
                     /locus_tag="ACH3L1_004306"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFT4078772.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
BASE COUNT          994 a          969 c          887 g          974 t
ORIGIN      
        1 gtaaatacag ccataacatc gtatggttct cagtgaacca ttttcaggat aactttctat
       61 gaaagtaaac ttaatacttt tcagcttatt tttattggtc tctattatgg catgcaatgt
      121 ttttgcattt tccatttctg gtggtgtaag tgagaggagc tataaagaga ctgaaaaaac
      181 atcagcgatg acgaccacac actctacaaa acttcagcca tcacaggcga ttttgtttaa
      241 gatgagagaa gatgtgccac cattaaacct cacagaagaa atcacgccta cttatccgac
      301 aaaggcgaat tatcttattc atcctgtgcg atagcgtctg tcacatggca ggtgaacaat
      361 catgtctgat gctgatgtga acacctgccc aaatgagcca tcctgaccca ctatcgcctg
      421 ccatacccca ccataaagcg aatcatcaag ccacaagccg gggcaagaaa cataaatatc
      481 cccgtcacca gttcattacc gccagttaac cacatggtga agtaataaat gagtgcagcg
      541 aggatcacca tgccgaagaa agaagagacc gccgatacca cgttcagtac ctgaccgatg
      601 tagccgctta cgcagacaat acacagggcg agaaacatcc ataaataaat catagctcaa
      661 cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt aatagtaaga caatccttaa
      721 cctctggcaa tttttgctaa gcatcaatga aaacagcacg ttaaatgcaa cgctattgcg
      781 tgcggaaaat tttttacttt gcacgattaa taatcagtag ctgaaagcag tcagccagtg
      841 ataaagtaga gccaccgcat tcatgctcct cgtggttatg tcctgactag tctttacact
      901 ctttacagga accattgtcg tacatgatgg cccaaccaat tgagtgtacc gctgtcgaca
      961 tggaatcttc tgcgatacaa caattcgtat ctacagaagg taactatgtt tccacaatgc
     1021 aaattttccc gcgagtttct acatcctcgc tactggctca catggtttgg gcttggtgta
     1081 ctctggcttt gggtacagct tccttatcct gttctctgct ttctcggcac gcgtattggc
     1141 gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg cccgtaaaaa cctggaactt
     1201 tgtttcccgc agcattctgc ggaagaacgc gagaagatga ttgccgaaaa ctttcgttca
     1261 ctcggcatgg cgctggtaga aaccggcatg gcatggttct ggcccgacag tcgcgtacgt
     1321 aaatggtttg atgttgaagg gttggataac cttaaacgcg cacaaatgca aaatcgcggc
     1381 gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg gcggccgcgt gatgggactg
     1441 tgccaaccaa tgatggctac ctatcgtcca cataataatc agctgatgga atgggtgcag
     1501 acccgtgggc gcatgcgctc taacaaagcg atgatcggca gaaataatct gcgcggcatt
     1561 gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc ccgatcagga ttatggtcgt
     1621 aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg tcgccacaac caatggcacc
     1681 tatgttctct cccgtctctc tggcgcagcc atgttgaccg taacgatggt aagaaaagcg
     1741 gattacagcg gatatcgttt gttcatcacc ccagagatgg aaggctaccc gacagatgaa
     1801 aatcaagccg ctgcttatat gaacaagatt atcgagaaag agatcatgcg cgcaccggag
     1861 cagtacctct ggatccaccg tcgctttaaa acgcgcccgg tgggagaatc gtcgttgtac
     1921 atttaacaaa ctgctcttac tctttccctc ctcaaatcgg ccataataac ctcatgattt
     1981 ttatagcttt tttcttaagt tttttccttc gctgaatatc taattattac cgtcagaaat
     2041 taaactgtcg ccgatatgag acacttgtaa gttactaaaa gtaaataaga aaatgcctct
     2101 tgtctcgttt cagttttagg cgtacattag cgccgtctcg agcagtttag ctcagaattc
     2161 tggggtgatg cccacggtaa aatacagggc ttttaaggtc gctttgcccg gtgtcaactc
     2221 actgtatcag gtgtaatgaa gtcattcagg cgtaacagta attacgcgga gagatgtaaa
     2281 gtgaaatatt tctttatggg catttctttt atggtcatcg tttgggccgg tacttttgcc
     2341 ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag ccatcaggct cctgtttgca
     2401 tttctgacga cggtgtttat tccgcgtttt cgtgaatatg tttgctgctg acgggtagta
     2461 aaccgtcggc acggaacatc gctttaatcc ctctgatggc ctgacgaata cggtcgtggt
     2521 tttcaatcag tgcaaagcga acatgcgtat caccatagtc gccaaagcca atccccggcg
     2581 agacacaaac cttcgcttcg ttaaggagct tcttggcaaa ttccagcgat cccatggccg
     2641 catatggttc cgggattttc gcccagacat acatcgaagc cttcggcatt tcgaccatcc
     2701 agcccgcttc atgcagccct ttaaccaata catcacgacg gcgtttgtac tgttcggcaa
     2761 tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat cgccgccacc tgcaacggcg
     2821 taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag ggcactgacc agcgttttgt
     2881 tgccgaccat aaagccgata cgccagcccg ccatgttgta gcttttcgac agcgtaaaga
     2941 actcgaccgc cacatcgcgt gcacccggca cctgcataat tgacggcgct ttccagccat
     3001 cgtagacgat atcggcatag gccaggtcat ggaccaccag cacatcgtag catttcgcca
     3061 gcgccacaac cttttcgaag aactccagtt ccacgcattg cgcggttggg ttcgacggga
     3121 agccgaggat catcatcttc ggtttcggat aactttcacg aatggcgcgc tccagttcgt
     3181 tgaagaaatc gacaccttcc accagcggca ctgagcgtac ctgcgccccg gcaatcaccg
     3241 cgccataaat atggatcggg taacttggat tcggcaccag caccgtgtca ccatgatcca
     3301 gcgtcgccag catcagatgc gccaggccct ctttcgaacc aatagtgacg atggcttctg
     3361 attccgggtc gatttcaacg tcgtagcgat cctgatacca gcgggaaatg gcgcgacgta
     3421 accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc cgggcgctgg gccacagtac
     3481 ataatttttc gacgatatgc ggcggagtcg caccgtccgg gttacccatg ctgaaatcga
     3541 taatatcttc gccgcgccga cgcgcagcca ttttcagttc agcggtgatg ttaaaaacgt
     3601 agggcgggag acgatcaatg cgcgtaaagc gacgttcagg gcgagtgtca gccataattt
     3661 cctcagatta acgttagcgc ccggaccgtc cgagcgacgc tgccacaatg gtggctgttt
     3721 tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta gaacattttt atcaatggat
     3781 ttaaatgagg aactccactt caaaaacagg aaatttttct acgc
//
LOCUS       DAZXUL010000238         3806 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZXUL010000238 DAZXUL010000000
VERSION     DAZXUL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.239"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            437..2089
                     /gene="ushA"
                     /locus_tag="ACH3L1_004307"
     CDS             437..2089
                     /gene="ushA"
                     /locus_tag="ACH3L1_004307"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
                     /protein_id="HFT4078773.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="ACH3L1_004308"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="ACH3L1_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="ACH3L1_004309"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="ACH3L1_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3595)
                     /locus_tag="ACH3L1_004310"
                     /pseudo
     CDS             complement(2596..3595)
                     /locus_tag="ACH3L1_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAZXUL010000239         3804 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZXUL010000239 DAZXUL010000000
VERSION     DAZXUL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.240"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            46..1458
                     /gene="ospC1"
                     /locus_tag="ACH3L1_004311"
     CDS             46..1458
                     /gene="ospC1"
                     /locus_tag="ACH3L1_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFT4078774.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(1562..1711)
                     /locus_tag="ACH3L1_004312"
                     /pseudo
     CDS             complement(1562..1711)
                     /locus_tag="ACH3L1_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1787..3484
                     /gene="ospD3"
                     /locus_tag="ACH3L1_004313"
     CDS             1787..3484
                     /gene="ospD3"
                     /locus_tag="ACH3L1_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFT4078775.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            3601..3720
                     /locus_tag="ACH3L1_004314"
                     /pseudo
     CDS             3601..3720
                     /locus_tag="ACH3L1_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         1325 a          664 c          702 g         1113 t
ORIGIN      
        1 agggaaagat attaattaaa actgttttca tataaggttc attttatgaa tatatcagaa
       61 acactgaact cagcaaatac ccaatgcaat atagattcta tggataacag attacataca
      121 ttgtttccaa aagtgacatc agtgcgaaac gctgcacaac aaactatgcc agatgaaaaa
      181 aatttaaaag atagtgcaaa tattattaaa agtttcttta ggaaaactat agcagcacag
      241 agttatagta gaatgttctc tcaaggctct aactttaaat ctttaaatat agcaattgat
      301 gcaccatcag acgctaaagc ctcatttaag gctattgagc accttgacag attatcgaag
      361 cattatatat ctgaaataag ggaaaaactt catcctcttt ctgcagagga actcaatttg
      421 ctttcgctaa ttattaattc tgatttaatc ttcagacatc aaagtaattc tgatttgtct
      481 gataaaattt taaacattaa gtcattcaat aaaattcagt ctgaaggaat atgcacaaaa
      541 cgaaacacat acgctgatga tataaaaaaa atagctaatc atgactttgt gttttttggc
      601 gttgaaatct ctaaccatca gaaaaaacac cccctgaata caaaacatca cactgttgat
      661 tttggtgcaa atgcgtatat cattgatcat gactctccat atggatatat gacattaacc
      721 gatcactttg ataatgctat tccacctgtt ttttaccatg agcaccaatc atttttagat
      781 aaattttcag aggttaataa agaagttagt cgatacgtac atggaagtaa aggaattata
      841 gatgtaccaa tattcaatac taaagatatg aagttagggc tcggattata cctgattgac
      901 tttattagaa aaagtgaaga ccaaagcttc aaggagtttt gctatggaaa aaatcttgcc
      961 cctgtggatc tggatagaat cataaacttt gtttttcagc cagagtacca tatacctagg
     1021 atggtaagta cagaaaactt caaaaaagtt aagattagag aaatatcctt agaggaggct
     1081 gttacagcat ctaattacga agaaattaac aagcaggtca ctaacaaaaa aattgctctc
     1141 caggctcttt ttctttcgat tactaatcaa aaagaggatg tcgccttata tatattatct
     1201 aattttgaga taactagaca agatgttatt tccataaagc atgagttgta tgatattgag
     1261 tatctactta gcgctcataa ttcaagctgt aaagtacttg agtattttat caataaggga
     1321 ttggttgatg taaacacaaa gttcaaaaaa actaatagtg gggattgtat gttggataac
     1381 gcaataaaat atgagaatgc agaaatgata aaactattat tgaaatatgg tgcaacatct
     1441 gacaataaat atatttaatc aaaattgaat atcgtttagc aaaaggcagt atttgcacaa
     1501 aatatcgata tattgtttat tgtcagtatg gctcaatgtg ataatagttg gaaagtttga
     1561 tgggtttcgc cccgttgtag cggtagtcga actggaaggt cttcaggcac tgcttacagc
     1621 gatagagcag cccccctgaa ctggaatggc cgttccggat acccccctga gtttcagagt
     1681 aacggtgaca aaccacatca atctttgcca tcaatcatcc aaagggcaaa gagtacaaca
     1741 acactaagtc tgcgtcacaa cccatcaatg aaaggaatat atacatatgc catcagtaaa
     1801 tttaatccca tcaaggaaaa tatgtttgca aaatatgata aataaagaca acgtctctgt
     1861 tgagacaatc cagtctctat tgcactcaaa acaattgcca tatttttctg acaagaggag
     1921 ttttttatta aatctaaatt gccaagttac cgatcactct ggaagactta ttgtctgtcg
     1981 acatttagct tcctactgga tagcacagtt taacaaaagt agtggtcacg tggattatca
     2041 tcactttgct tttccggatg aaattaaaaa ttatgtttca gtgagtgaag aagaaaaggc
     2101 tattaatgtg cctgctatta tttattttgt tgaaaacggt tcatggggag atattatttt
     2161 ttatattttc aatgaaatga tttttcattc cgaaaaaagc agagcactag aaataagtac
     2221 atcaaatcac aatatggcat taggcttgaa gattaaagaa actaaaaatg ggggggattt
     2281 tgtcattcag ctttatgatc ccaaccatac agcaactcat ttacgagcag agtttaacaa
     2341 atttaactta gctaaaataa aaaaactgac tgtagataat tttcttgatg aaaaacatca
     2401 gaaatgttat ggtcttatat ccgacggtat gtctatattt gtggacagac atactccaac
     2461 aagcatgtcc tccataatca gatggcctga taatttactt caccccaaag ttatttatca
     2521 cgcgatgcgt atgggattga ctgagctaat ccaaaaagta acaagagtcg tacaactatc
     2581 tgacctttca gacaatacgt tagaattact tttggcagcc aaaaatgacg atggtttgtc
     2641 aggattgctt ttagctttac aaaatgggca ttcagataca atcttagcat acggagaact
     2701 cttggaaact tctggactta accttgataa aacggtagaa ctactaactg cggaaggaat
     2761 gggaggacga atatcgggtt tatcccaagc acttcaaaat gggcatgcag aaactatcaa
     2821 aacatacgga aggcttctca agaagagagc aataaatatc gaatacaata agctgaaaaa
     2881 tttgctgacc gcttattatt atgatgaagt acacagacag atacccggac taatgtttgc
     2941 tcttcaaaat ggacatgcag atgctatacg cgcatacggt gagctcattc ttagcccccc
     3001 tctcctcaac tcagaggata ttgtaaattt gctggcctca aggagatatg acaatgttcc
     3061 cggacttctg ttagcattga ataatggaca ggctgatgca atcttagctt atggtgatat
     3121 cttgaatgag gcaaaactta acttggataa aaaagcagag ctgttagaag cgaaagattc
     3181 taatggttta tctggattgt ttgtagcctt gcataatgga tgtgtagaaa caattattgc
     3241 ttatgggaaa atacttcaca ctgcagacct tactccacat caggcatcaa aattactggc
     3301 agcagaaggc ccaaatgggg tatctggatt aattatagct tttcaaaata ggaattttga
     3361 ggcaataaaa acttatatgg aaataataaa aaatgaaaat attacacctg aagaaatagc
     3421 agaacacttg gacaaaaaaa atggaagtga ttttctagaa attatgaaga atataaaaag
     3481 ctgaatatta tctcgtggtg atacactgaa tttgaggtaa ccggctcatt taaaccgtct
     3541 ggtctgttac ctccggttcc acaaaaataa tgtccatcat tttgaagtgg acactatcgt
     3601 atggaatacc ggacctggat cactgaagct ttacgccttc atttcgaaga acatttacca
     3661 cgggttgtgg ccggacgtcg cctgggcgta ccaaaatcaa ctgtctgtgg tatgttcgtg
     3721 tgaaccgccc ccggtatccc gctcatgctg gcgaacccag acacgcagtg aaccgccccg
     3781 ggtttcctgg agagtgtttt atct
//
LOCUS       DAZXUL010000240         3790 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZXUL010000240 DAZXUL010000000
VERSION     DAZXUL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.241"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(161..205)
                     /locus_tag="ACH3L1_004315"
     CDS             complement(161..205)
                     /locus_tag="ACH3L1_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078776.1"
                     /translation="MKRNDKILHYEGLN"
     gene            324..2048
                     /gene="ilvI"
                     /locus_tag="ACH3L1_004316"
     CDS             324..2048
                     /gene="ilvI"
                     /locus_tag="ACH3L1_004316"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFT4078777.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            2051..2542
                     /gene="ilvN"
                     /locus_tag="ACH3L1_004317"
     CDS             2051..2542
                     /gene="ilvN"
                     /locus_tag="ACH3L1_004317"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFT4078778.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2554..2640
                     /locus_tag="ACH3L1_004318"
     CDS             2554..2640
                     /locus_tag="ACH3L1_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFT4078779.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2722..3726
                     /gene="cra"
                     /locus_tag="ACH3L1_004319"
     CDS             2722..3726
                     /gene="cra"
                     /locus_tag="ACH3L1_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFT4078780.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERMLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          892 a          893 c         1040 g          965 t
ORIGIN      
        1 cgctaactga atgcagaata ggccagacat gaatgtctgg tttattctgc attttttatt
       61 gaatgtagaa ttttattctg aatgtgtgga ctctctattt taggattaat taaaaaaata
      121 gagaaattgc tgtaagttgt gggattcagc cgatttatta tcaatttaat ccttcgtaat
      181 ggaggatttt atcgtttctt ttcacctttc ctcctgttta ttcttattac cctgcgttta
      241 tgtctctggc tgccaattgc ttaagcaaga tcggacggtt aatgtgtttt acacattttt
      301 tccgtcaaac agtgaggcag gccatggaga tgttgtctgg agccgagatg gtcgtccgat
      361 cgcttatcga tcagggcgtt aaacaagtat tcggttatcc cggaggcgca gtccttgata
      421 tttacgatgc attgcatacc gtgggtggta ttgatcatgt attagttcgt catgagcagg
      481 cggcggtgca tatggccgat ggcctggcgc gtgcgaccgg ggaagtcggc gtcgtgctgg
      541 taacgtcggg tccaagggcg accaatgcga ttaccggcat cgccaccgct tatatggatt
      601 ccattccatt agttgtcctt tccgggcagg tagcgacctc gttgataggt tacgatgcct
      661 ttcaggagtg cgacatggtg gggatttcgc gaccggtggt taaacacagt tttctggtta
      721 agcaaacgga agacattccg caggtgctga aaaaggcttt ctggctggcg gcaagtggtc
      781 gcccaggacc agtggtcgtt gatttaccga aagatattct taatccggcg aacaaattac
      841 cctatgtctg ccctgagtcg gtcagtatgc gttcttacaa tcccactact accggacata
      901 aagggcaaat taagcgtgct ctgcaaacgc tggtagcggc aaaaaaaccg gttgtctacg
      961 taggcggtgg ggcaatcatg gcgggctgcc atcagcagtt gaaagaaacg gtggaggcgt
     1021 tgaatctgcc cgttgtttcc tcattgatgg gaatgggggc gtttccggca acgcatcgtc
     1081 aggcactggg catgctggga atgcacggta cctacgaagc caatatgacg atgcataacg
     1141 cggatgtgat tttcgccgtc ggggtacgat ttgatgaccg aacgacgaac aatctggcaa
     1201 agtactgccc aaatgccact gttctgcata tcgatattga tcctacttcc atttctaaaa
     1261 ccgtgactgc ggatatcccg attgtggggg atgctcgcca ggtcctcgaa caaatgcttg
     1321 aactcctgtc gcaagaatcc gcccatcaac cgctggatga gatccgcgac tggtggcagc
     1381 agattgaaca gtggcgcgct cttcagtgcc tgaaatatga cactcacagt gaaaagatta
     1441 aaccgcaggc ggtgatcgag actctttggc ggttgactaa tggggacgct tacgtgacgt
     1501 ccgatgtcgg gcagcaccag atgtttgctg cgctttatta tccattcgac aaaccgcgtc
     1561 gctggatcaa ttccggcggt ctcggcacga tgggttttgg tttacctgcg gcactgggcg
     1621 tcaaaatggc gttgccagaa gaaaccgtgg tttgcgtcac tggcgacggc agtattcaga
     1681 tgaacatcca ggaactgtct accgcgttgc aatacgagtt gcccgtactg gtggtgaatc
     1741 tcaataaccg ctatctgggg atggtgaaac agtggcagga catgatctat tccggccgtc
     1801 attcacaatc ttatatgcaa tcactacccg atttcgtccg tctggcggaa gcctatggtc
     1861 acgtcgggat ccagatctct catccgcatg agctggaaag caaacttagc gaggcgctgg
     1921 aacaggtgcg caataatcgc ctggtgtttg ttgatgttac cgtcgatggc agtgagcacg
     1981 tctacccgat gcagattcgc gggggcggaa tggatgaaat gtggttaagc aaaacggaga
     2041 gaacctgatt atgcgccgga tattatcagt cttactcgaa aatgaatcag gcgcgttatc
     2101 ccgcgtgatt ggcctttttt cccagcgagg ctacaacatt gaaagcctga ccgttgcgcc
     2161 aaccgacgat ccgacattat cgcgtatgac catccagacc gtgggcgatg aaaaagtact
     2221 tgagcagatc gaaaagcaat tacacaagct ggtcgatgtc ttgcgcgtga gtgagttggg
     2281 gcagggcgcg catgttgagc gggaaatcat gctggtgaaa attcaggcca gcggttacgg
     2341 gcgtgacgaa gtgaaacgta atacggaaat attccgtggg caaattatcg atgtcacccc
     2401 ctcgctttat accgttcaat tagcaggcac cagcgataag cttgatgcat ttttagcatc
     2461 gattcgcgat gtggcgaaaa ttgtggaagt tgctcgctct ggtgtggtcg gactttcgcg
     2521 cggcgataaa ataatgcgtt gagaatgatc tcagtgcgca atttgcagcc caacatgtca
     2581 cgttgggctt tttttgcgaa atcagtggga acctggaata aaagcagttg ccgcagttaa
     2641 ttttctgcgc ttagatgtta atgaatttaa cccataccag tacaatggct atggttttta
     2701 cattttacgc aaggggcaat tgtgaaactg gatgaaatcg ctcggctggc gggagtgtcg
     2761 cggaccactg caagctatgt tattaacggc aaagcgaagc aataccgtgt gagcgacaaa
     2821 accgttgaaa aagtcatggc tgtggtgcgt gagcacaatt accacccgaa cgccgtggca
     2881 gctgggcttc gtgctggacg cacacgttct attggtcttg tgatccccga tctggagaac
     2941 accagctata cccgcatcgc taactatctt gaacgccagg cgcggcaacg gggttatcaa
     3001 ctgctgattg cctgctcaga agatcagcca gacaacgaaa tgcgctgcat tgagcacctt
     3061 ttacagcgtc aggttgatgc cattattgtt tcgacgtcgt tgccacctga gcatcctttt
     3121 tatcaacgct gggctaacga cccgttcccg attgtcgcgc tggaccgcgc cctcgatcgt
     3181 gaacatttca ccagcgtggt tggtgccgat caggatgatg ccgaaatgct ggcggaagag
     3241 ttacgtaagt ttcccgccga gacggtgctt tatcttggcg cgctgccgga gctttctgtc
     3301 agcttcctgc gtgaacaagg tttccgtact gcttggaaag atgatccgcg cgaagtgcat
     3361 ttcctgtatg ccaacagcta tgagcgggag gcggctgccc agttattcga aaaatggctg
     3421 gaaacgcatc cgatgccgca ggcgctgttc acaacgtcgt ttgcgttgtt gcaaggagtg
     3481 atggatgtca cgctgcgtcg cgacggcaaa ctgccttctg acctggcaat tgccaccttt
     3541 ggcgataacg aactgctcga cttcttacag tgtccggtgc tggcagtggc tcaacgtcac
     3601 cgcgatgtcg cagagcgtat gctggagatt gtcctggcaa gcctggacga accgcgtaag
     3661 ccaaaacctg gtttaacgcg cattaaacgt aatctctatc gccgcggcgt gctcagccgt
     3721 agctaatccg cgaacaaaaa tacgcgccag gtgaatttcc ctctggcgcg tagagtacgg
     3781 gactggacat
//
LOCUS       DAZXUL010000241         3738 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZXUL010000241 DAZXUL010000000
VERSION     DAZXUL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.242"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            108..2105
                     /locus_tag="ACH3L1_004320"
     CDS             108..2105
                     /locus_tag="ACH3L1_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFT4078781.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="ACH3L1_004321"
     CDS             2168..3445
                     /locus_tag="ACH3L1_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFT4078782.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          904 a          852 c          941 g         1041 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg tcattgatcc tacccacgta atatggacac
     3721 aggcctaagc gaggttct
//
LOCUS       DAZXUL010000242         3659 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZXUL010000242 DAZXUL010000000
VERSION     DAZXUL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.243"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            128..1279
                     /gene="yiaY"
                     /locus_tag="ACH3L1_004322"
     CDS             128..1279
                     /gene="yiaY"
                     /locus_tag="ACH3L1_004322"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFT4078783.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            1444..2982
                     /gene="aldB"
                     /locus_tag="ACH3L1_004323"
     CDS             1444..2982
                     /gene="aldB"
                     /locus_tag="ACH3L1_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFT4078784.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3201..3344)
                     /locus_tag="ACH3L1_004324"
     CDS             complement(3201..3344)
                     /locus_tag="ACH3L1_004324"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078785.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3527..>3659
                     /locus_tag="ACH3L1_004325"
     CDS             3527..>3659
                     /locus_tag="ACH3L1_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFT4078786.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT          919 a          864 c          949 g          927 t
ORIGIN      
        1 acattgttta atgaatacaa tgtgcttttt attagattaa ttttgataat taataataat
       61 gatcatgttt tcgaatgaaa atcctcagta agctgcccac ccttttttac actttcagga
      121 gtgtgttatg gcatcttcaa ctttctttat tccttctgtg aatgtcatcg gcgctgattc
      181 attgactgat gcaatgaata tgatggcaga ttatggattt acccgtacct taattgtcac
      241 tgacaatatg ttaacgaaat taggtatggc gggtgatgtg caaaaagcac tggaagaacg
      301 caatattttt agcgttattt atgatggcac ccaacctaac cccaccacgg aaaacgtcgc
      361 cgcaggtttg aaattactta aagaaaataa ttgcgatagc gtgatctcct taggcggtgg
      421 ttctccacac gactgcgcaa aaggtattgc gctggtggca gccaatggcg gcgatattcg
      481 cgattacgaa ggcgttgacc gctctgcaaa accgcagctg ccgatgatcg ccatcaatac
      541 cacggcgggt acggcctctg aaatgacccg tttctgcatc atcactgacg aagcgcgtca
      601 taacaaaatg gcgattgttg ataagcacgt gactccgctg ctttctgtca atgactcctc
      661 tctgatgatt ggtatgccga agtcactgac cgccgcaacg ggtatggatg ccttaacgca
      721 cgctatcgaa gcgtatgttt ctattgccgc cacgccgatc actgacgctt gtgcactgaa
      781 agccgtgacc atgattgccg aaaacctgcc gttagccgtt gaagatggca gtaatgcgaa
      841 agcgcgtgaa gcaatggctt atgcccagtt ccttgccggt atggcgttca ataatgcttc
      901 tctgggttat gttcatgcga tggcgcacca gctgggcggt ttctacaacc tgccacacgg
      961 tgtatgtaac gccgttctgc tgccgcatgt tcaggtattc aacagcaaag tcgccgctgc
     1021 acgtctgcgt gactgtgccg ctgcaatggg cgtgaacgtg acaggtaaaa acgacgcgga
     1081 aggtgctgaa gcctgcatta acgccatccg tgaactggcg aagaaagtgg atatcccggc
     1141 aggcctacgc gacctgaacg tgaaagaaga agatttcgcg gttctggcga ctaatgccct
     1201 gaaagatgcc tgtggcttta ctaacccgat ccaggcaact cacgaagaaa ttgtggtgat
     1261 ttatcgcgcc gcaatgtaat catcattcca acaacggctg gcaaattgtc agccgctttt
     1321 tcaactatct ctgtaatcct tgcccgtaaa ttcgtgatag ctgtcgtaaa gctgttaccg
     1381 actggcgaag atttcgccag tcacgtctac ccttgttata cctcacaccg caaggagacg
     1441 atcatgacca ataatccccc ttcagcacag attaagcccg gcgagtatgg tttccccatc
     1501 aagttaaaag cccgctacga caactttatt ggcggtgaat gggtagcccc tgccgatggt
     1561 gagtattacc agaatctgac gccggtgacc gggcagctgc tgtgcgaagt ggcgtcttcg
     1621 ggcaaacgag acatcgatct ggcgctggat gctgcgcaca aagtgaaaga taaatgggcg
     1681 cacacctcgg tgcaggatcg cgcggcgatt ctgtttaaga ttgccgatcg aatggaacaa
     1741 aacctcgagc tgttagcgac agctgaaacc tgggataacg gcaaacccat tcgcgaaacc
     1801 agtgctgcgg atgtaccgct ggcgattgac catttccgct atttcgcctc gtgtattcgg
     1861 gcgcaggaag gtgggatcag tgaagttgat agcgaaaccg tggcctatca tttccatgaa
     1921 ccgttaggcg tggtggggca gattatcccg tggaacttcc cgctgctgat ggcgagctgg
     1981 aaaatggctc ccgcgctggc ggcgggcaac tgtgtggtgc tgaaacccgc acgtcttacc
     2041 ccgctttctg tactgctgct aatggaaatt gtcggtgatt tactgccgcc gggcgtggtg
     2101 aacgtggtca atggcgcagg tggggaaatt ggcgaatatc tggcgacctc gaaacgcatc
     2161 gccaaagtgg cgtttaccgg ctcaacggaa gtgggccaac aaattatgca atacgcaacg
     2221 caaaacatta ttccggtgac gctggagttg ggcggtaagt cgccaaatat cttctttgct
     2281 gatgtgatgg atgaagaaga tgcctttttc gataaagcgc tggaaggctt tgcactgttt
     2341 gcctttaacc agggcgaagt ttgcacctgt ccgagtcgtg ccttagtgca ggagtcaatc
     2401 tacgaacgct ttatggaacg cgccatccgc cgtgtcgaaa gcattcgcag cggtaacccg
     2461 ctcgacagcg tgacgcaaat gggcgcgcag gtttctcacg ggcaactgga aaccatcctc
     2521 aactacattg atatcggtaa aaaagagggc gctgacgtgc tcacaggcgg gcggcgcaag
     2581 ctgctggaag gtgaactgaa agacggctac tacctcgaac cgacgattct gtttggtcag
     2641 aacaatatgc gggtgttcca ggaggagatt tttggcccgg tgctggcggt gaccaccttc
     2701 aaaacgatgg aagaagcgct ggagctggcg aacgatacgc aatatggcct gggcgcgggc
     2761 gtctggagcc gcaacggtaa tctggcctat aagatggggc gcggcataca ggctgggcgc
     2821 gtgtggacca actgttatca cgcttacccg gcacatgctg cgtttggtgg ctacaaacaa
     2881 tcaggtatcg gtcgcgaaac ccacaagatg atgctggagc attaccagca aaccaagtgc
     2941 ctgctggtga gctactcgga taaaccgttg gggctgttct gatataagaa gctggtcgca
     3001 ttgggtattc attgcctgat gcgacgctta cgcgtcttat catgcctacg ggaacctgac
     3061 cgtaggccgg ataaggcgta cacgccgcat ccggcataaa caaagcgcac gttgttacaa
     3121 tttacgtttt aaacctacgt gcgcaataac ccagactacg ccaacagaag tgcgtttctg
     3181 gggcgtgttt tgtcaggagt ttaatctcgc gtttcgatgg tgagcgggcg aaccaggccg
     3241 taaatcacca ttggtgatcc gaggccaatc agcaataaac ggtagataat tgaaggttta
     3301 atacgcttaa tctgtactgt ggatgtttta ctggagttat tcattagttt ggccaatagt
     3361 tccactattt caatatgata aacctcgtcc tgttgaaaaa ccagacagca catttcagaa
     3421 agtatataaa aaacaatatg tcgttatata aataaggaaa ttgcactgca tcatggcatt
     3481 tcataatatg cgttaattgt tcttttttat attctttgag ggaaatatgt tcctggacta
     3541 ttttgcactg ggagtgctta tttttgtatt tctggtgata ttctatggga ttattatttt
     3601 acatgatatt ccctacctga ttgctaaaaa acgtaatcat cctcatgccg acgccattc
//
LOCUS       DAZXUL010000243         3568 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZXUL010000243 DAZXUL010000000
VERSION     DAZXUL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.244"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..77
                     /locus_tag="ACH3L1_004326"
     CDS             <1..77
                     /locus_tag="ACH3L1_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFT4078787.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            435..1493
                     /gene="rfaQ"
                     /locus_tag="ACH3L1_004327"
     CDS             435..1493
                     /gene="rfaQ"
                     /locus_tag="ACH3L1_004327"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFT4078788.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1490..2614
                     /locus_tag="ACH3L1_004328"
     CDS             1490..2614
                     /locus_tag="ACH3L1_004328"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFT4078789.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2607..3404
                     /gene="rfaP"
                     /locus_tag="ACH3L1_004329"
     CDS             2607..3404
                     /gene="rfaP"
                     /locus_tag="ACH3L1_004329"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFT4078790.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          948 a          768 c          860 g          992 t
ORIGIN      
        1 cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat tatctgaaca
       61 taaaacacta tcagtaagtt ggagtcatta cccgtttttg acttaaatag caatttaccc
      121 gccaattctt gttgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg
      181 ctgtacgtgc aggaaggaat cgcagagcaa cagtgaaaga atatggttgc taatgatttt
      241 atttgattga tttctttatt gaaaatcaac aatgtgctta aaaatcattc gggtcggatt
      301 gccgcgaaag aagtgataca ctagcgcgtc aatatcagtg cggtatcagt attcaggtag
      361 ctgttgagcc tggggcggta gcgtgctttt tctgcttcac cttaatcgga taatctcaac
      421 aaaaagagtt acttgtggat aagccatttc gaagaatttt gctcattaag atgcgttttc
      481 atggggatat gttattaact actcccgtca ttagttctct gaaaaaaaat taccctgacg
      541 caaaaatcga tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa aatccagaga
      601 ttaacgcgct ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact
      661 tttttcatct catcaaggta ttacgtgcca ataagtatga ccttatcgtc aatcttaccg
      721 atcaatggat ggttgctata ctggttcgct tattaaatgc tcgtgtgaaa atttcccagg
      781 attatcatca tcggcagtct gctttttggc gtaaaagttt cacccatttg gtgccgttgc
      841 agggtggaaa tgtggtggaa agtaacttat ccgtgctgac accattggga cttgattcgt
      901 tggtgaagca gacaaccatg agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac
      961 ttgatcacgc tggtgttgga caaaattatg tggttatcca acctacggcg cggcaaatct
     1021 tcaaatgctg ggacaacgcc aagttttccg ctgtgattga tgccttacat gctcgtggtt
     1081 atgaagtcgt tctgacgtcc ggcccagata aagacgatct ggcctgcgtc aatgaaattg
     1141 cgcagggatg ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac
     1201 ttggtgcgtt aatcgatcat gcgcagctgt ttattggcgt tgattccgca ccggcgcata
     1261 ttgccgctgc agttaatacg ccgctgatat cgctgtttgg cgcaacagat catattttct
     1321 ggcgtccctg gtcaaataac atgattcaat tctgggcggg agattaccgg gaaatgccaa
     1381 cgcgcgatca gcgtgaccga aatgagatgt atctttcggt tattccggcg gcagatgtca
     1441 ttgctgctgt cgataaatta ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc
     1501 tttttgttta tataaatatt ttccctttgg cggtttgcag cgcgatttta tgcgtattgc
     1561 tcagacagtc gccgcccgag gtcatcatgt tcgggtttat acccagtcgt gggaaggcga
     1621 atgccctgat gtatttgaag tgatcaaagt gccggttaaa tcgcatacca atcacgggcg
     1681 caatgcggag tattttgcct gggtgcaaaa acatttacgc gaacatcccg tcgataaagt
     1741 cgttggattc aacaaaatgc cggggctgga cgtttattat gccgctgatg tttgttatgc
     1801 cgagaaagta gcgcaggaaa aaggcttttt ctatcgcctg acgtcacgtt atcgccatta
     1861 tgccgccttt gagcgggcaa ccttcgaaca gggcaagccg acacagctgc tgatgctgac
     1921 agataagcaa atcgccgatt tccagaaaca ttatcagact gaagcggagc gttttcatat
     1981 tctgccaccg gggatttatc ctgatcgtaa atatagccag cagccagcca atagccgtga
     2041 aatcttccgt aagaagaatg gaataaccga acaacaatat ttattgttgc aggtcggttc
     2101 agacttcacg cgtaaaggtg ccgatcgttc cattgaagca cttgcttcgt taccggattc
     2161 gctgcgccac aacacattgc tatatgttgt tgggcaggat aaaccgcgaa aatttgaggc
     2221 actggcagaa aaacgcggcg tgcgcagtaa tgttcacttc ttctcggggc gcaacgatgt
     2281 ctcggaatta atggcggcgg cggatttatt actgcatcct gcctaccagg aagcggcggg
     2341 aattgtgctg ctggaagcga ttactgcagg attaccggta ctaacaactg ccgtttgtgg
     2401 ctatgcgcat tatattgtcg acgctaattg cggcgaggct attgctgagc cattccgcca
     2461 ggaaacattg aatgagattt tacgcaaagc gttaacgcaa tcttcattgc gccaggcttg
     2521 ggcggaaaat gcgcgacatt atgctgatac acaagattta tacagtctgc cagagaaagc
     2581 ggcggatatc ataacgggtg gtctggatgg ttgaacttaa agagccgttt gccacgttat
     2641 ggcgcggcaa agatcctttt gaggaagtta aaaccttgca gggtgaggta tttcgtgaac
     2701 tggaaactcg ccgtactctg cgctttgaaa tggcgggaaa aagctatttt ctcaaatggc
     2761 atcgcggcac gaccctgaaa gagataatca aaaatttact ctcattgcgg atgccagtat
     2821 taggcgcaga ccgcgaatgg aatgcgattc atcgactgcg ggatgtcggc gttgatacta
     2881 tgtatggggt ggcattcggc gaaaaaggca tgaatccgct gaccagaacc tcgtttatta
     2941 ttaccgaaga tctgacacca accatcagtc tggaagatta ctgtgctgac tgggcgacca
     3001 acccaccaga tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg atggtgcgcg
     3061 atatgcatgc tgcgggcatt aaccatcgtg actgttatat ctgtcatttc ctgctgcact
     3121 tgcctttttc cggtaaggaa gaggagttaa aaatttcggt aattgaccta caccgggcgc
     3181 agcttcgcac gcgcgttcca ggtcgttggc gcgataaaga tcttataggg ctttattttt
     3241 cttcaatgaa tattggcctg actcagcggg atatctggcg ctttatgaag gtgtattttg
     3301 ccgccccgct taaagacatt ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa
     3361 aagccacaaa aatcagggaa agaacgattc gaaaatcgtt gtaacccgta acttattttt
     3421 gccaaaattt tggatacaga ataaatatgt ctcaactcaa tgatagtgac atcatccttt
     3481 ttgagtataa ttttcattat caaaatataa gatctaaaaa tactcttgat atagcatttg
     3541 gtattgacag aaattttctt tttggatg
//
LOCUS       DAZXUL010000244         3563 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZXUL010000244 DAZXUL010000000
VERSION     DAZXUL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.245"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..903
                     /locus_tag="ACH3L1_004330"
     CDS             <1..903
                     /locus_tag="ACH3L1_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFT4078791.1"
                     /translation="PKGRDILREAPEMKYVFIEKHQAEFSIKAMCRVLRVARSGWYTW
                     CQRRTRISTRQQFRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAASLR
                     RQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWPGDITYLRTDEGWLYL
                     AVVIDLWSRAVIGWSMSPRMTAQLPCDALQMALWRRKRPRNVIVHTDRGGQYCSADYQ
                     AQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECD
                     YNRWRRHSWCGGLSPEQFENQNLA"
     gene            complement(935..1480)
                     /gene="ygeQ"
                     /locus_tag="ACH3L1_004331"
                     /pseudo
     CDS             complement(935..1480)
                     /gene="ygeQ"
                     /locus_tag="ACH3L1_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
     gene            complement(2142..3131)
                     /locus_tag="ACH3L1_004332"
     CDS             complement(2142..3131)
                     /locus_tag="ACH3L1_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078792.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(3215..>3563)
                     /locus_tag="ACH3L1_004333"
     CDS             complement(3215..>3563)
                     /locus_tag="ACH3L1_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078793.1"
                     /translation="TSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAER
                     IAKELASQKERTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIV
                     ADLESGGNWMDSL"
BASE COUNT          903 a          852 c          908 g          900 t
ORIGIN      
        1 ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga agtatgtctt tattgaaaaa
       61 catcaggctg agttcagcat caaagcaatg tgccgcgtgc tccgggtggc ccgcagcggc
      121 tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc gtcagcagtt ccgccaacac
      181 tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac agcgttacgg tgccccacgc
      241 ctgacggatg aactgcgtgc tcagggttac ccctttaacg taaaaaccgt ggcggcaagc
      301 ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt tcagcccggt cagctaccgc
      361 gcacacggcc tgcctgtgtc agaaaatctg ttggagcagg atttttacgc cagtggcccg
      421 aaccagaagt ggccaggaga catcacgtac ttacgtacag atgaaggctg gctgtatctg
      481 gcagtggtca ttgacctgtg gtcacgtgcc gttattggct ggtcaatgtc gccacgcatg
      541 acggcgcaac tgccctgcga tgccctgcag atggcgctgt ggcggcgtaa gaggccccgg
      601 aacgttatcg ttcacacgga ccgtggaggc cagtactgtt cagcagatta tcaggcgcaa
      661 ctgaaacggc ataatctgcg tggaagtatg agcgcaaaag gttgctgcta cgataatgcc
      721 tgcgtggaaa gcttctttca ttcgctgaaa gtggaatgta tccatggaga acactttatc
      781 agccgggaaa taatgcgggc aacggtgttt aattatatcg aatgtgatta caatcggtgg
      841 cggcggcaca gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca gaacctcgct
      901 taggcctgtg tccatattac gtgggtagga tcagaattcc atccagtcgt tttgggttgc
      961 taatgatgtc ctgcatccgc ttaatctggt cgggcgtaga catataaccc cacagggtga
     1021 gcagaacctt ttttgcggct acactatttc cttggtctga tactaatgcc atatagcccc
     1081 ataaactatc cctcagccag acagcatcat agtgggtttc tgccgctgtt ttgtcatctt
     1141 cgaaagtaga agcaagaata aacgttcccc agggtttctc cgtcaacgtt aactttcgtg
     1201 tgttttctgt taatgttagc aatccttgag tttcgtcttt ggtatagtga ctctttattt
     1261 cattttttaa tttggctgag tgaatcagaa gacgataatt aggatactta tctggattta
     1321 acgataaata acttttatct gttgctgccc ccctggaatt aagaatgtta aatacagatt
     1381 cgcttgtcac ttctgcctct gtcgcctggc atgtgccgca cgaaaatata ccagcgagca
     1441 gaagagttaa tgcagatttc cctttcataa aataatccat caatcgtttg ttaaaagcgt
     1501 agataaccac ttattcaaca acttgaaaat aaggtatttt acctgagttg ttacaaaagg
     1561 attgcattgc gtaaacgctt tttatttaca aaaaaatggg gaagtgttac agcgattgcg
     1621 aaaagcctga agtgattgaa ttaagataat atagaaaatc agatacagca aaaatggcag
     1681 attgatcagg tgaagataaa ttggagcggg cgaagggagc ctacgacaaa tcgaagacaa
     1741 ccagctaacc tattgtttct taaagacagg tgaatccagt tggttgtagt gatagtcaca
     1801 catttagaga tagaaggtca agacttgaag cacgtaatat tccctctaaa ggaatagaac
     1861 tcacggtctg actacccggc ccttttggtc tggctcaggt caggctatgg gggcttttgg
     1921 ctcgctcttt atattagatg aggtctcaca tttttcagtc aaaactcatc gtctaatagc
     1981 ccatagccag accaccagtc ccagctttag caggaagacc acgagataga ctttgtgtag
     2041 gtccatttgg ccctcctact ttctgtattc acaaactaga ttactagtac aaaataaagg
     2101 ccagacatcc agttcggaag tctgaccaat agtcgttatt gttagttagc ttctttagtg
     2161 aagctcaggc gtttgactac aggcaggaca gcagtagccg agccagtacc catcttgatg
     2221 gtcgtagcag cttcagctac aacaaacacc ttgccgagaa tctcaaggtc gtgagactgg
     2281 gagtctccat cacgtgaaag gaccacagta atgctctcgc catcgtgacg gaagcaaccg
     2341 aagctcagag ccgtaaaacg cttagccatc atgaacacgc cacggtcttc accgttgtaa
     2401 taggcgacgt cagtccccag catatcgacg atagcgtctt caccaccgaa gccctgctta
     2461 gcagccagac gggagagagc cttgaggtgt gactcatggg cgataatgcc gtagtcagaa
     2521 acctcagaac cgaaagaaga ctccgtgttc tcgtacaggg catccatgat agcgtcagcc
     2581 ttagcggtca ggctaccagt cagggccgga agttctacgg tcttcagcag ctttgaggac
     2641 tccagcacgt caaccatctt agagttaatg gtacgaatca cgttatcgca gatgttcagt
     2701 gcgtcctgct ggagcgtccc ctgagtgaaa ccagtggtcg acttctggtc ccaaagcttt
     2761 gtttggtgag acacgaggat cgcctgttca atcggctcaa cggtgatttc agtgccagac
     2821 ggagcggtca caacttcagg tcgcttacca ccacggacag ggaccagcac agagcggtca
     2881 gccagagtgt gagcaatgat tggagtcggt aagctgttac gggtcggcag gacttctaca
     2941 gggaacggga tgttatcttg agcgtcttta agtacaccgt tcagcatagc cttaacagtc
     3001 ttagctaact ggagggcata gacctgaggg tcggcgttag tcggcagacc ttcattgacc
     3061 tgaatgttgt cggtatcggt gatgcgtact ggctcaatgc caaactggaa agcgttttta
     3121 gtcatattca ttataattaa tctccttgag tcaaatactt gaagtcccta acaggcagtc
     3181 agggaccgtg tgttctcaga tgagggacgc ttgcttacaa gctgtccatc cagttaccgc
     3241 ctgactcaag gtcagcgacg atagcggcac gtgagcgacc agcggaatga gtagcgctgc
     3301 ggaggttttc accataggtc cggcgttcag ctttgacctg ttcgcggcgc tgtagctctt
     3361 tttgccattc gtcttcggtc atgaacattt cgcgggtacg ctctttctga gacgctagct
     3421 ctttagcgat acgctcagct tcagctcgtt tgttcatctg gcggcgttgg taggcggtca
     3481 tcaggtcatt actgaagaag ttaccaatgg tcacgccagc ttcagccaga gcttccagta
     3541 actccatcgc ctcgcggctg gtg
//
LOCUS       DAZXUL010000245         3546 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZXUL010000245 DAZXUL010000000
VERSION     DAZXUL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.246"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..226
                     /locus_tag="ACH3L1_004334"
     CDS             <1..226
                     /locus_tag="ACH3L1_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFT4078794.1"
                     /translation="SPYLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYG
                     TPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            148..1266
                     /locus_tag="ACH3L1_004335"
     CDS             148..1266
                     /locus_tag="ACH3L1_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4078795.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHEE
                     NWRLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRV
                     AWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKM
                     RYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRY
                     AHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            1615..2787
                     /locus_tag="ACH3L1_004336"
     CDS             1615..2787
                     /locus_tag="ACH3L1_004336"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFT4078796.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2841..>3546)
                     /locus_tag="ACH3L1_004337"
     CDS             complement(2841..>3546)
                     /locus_tag="ACH3L1_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005078762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078797.1"
                     /translation="CEGNGTQGDSSTGGAQLAINGLNGSCALVLNNPYFEANAGGADL
                     AIDNTGTRPVTVVINGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTTFQSAGDYQ
                     PSAERPYWITGANCEVVDIGCVFTETTSKVTSASALSVTRSGKINANGSIDVATGVSS
                     VNVVSTGVYDVSFSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLS
                     GAGISSSFAFSITRLI"
BASE COUNT         1117 a          745 c          727 g          957 t
ORIGIN      
        1 atctccttac ctcacaacac gacaagcctg cattgcggct cttcagtctc cgctgcatac
       61 tgtccaggtg agcgcgggtg atggcataac agaggaaaga aaatgtcact cttccgcaga
      121 ggtgaaatat ggtacgccag tttcacattg ccgaacggta aaagatttaa acagtctctt
      181 ggaacaaagg acaaaaggca ggcgacagaa ctccatgaca agctaaaggc tgaagcatgg
      241 cgggtcagca aacttggtga aatacctgat ataacgttcg aggaagcgtg tgtcaggtgg
      301 cttgaagaga aagcacataa aaaatcactg gacgatgaca aaagccggat cggattctgg
      361 cttcaacatt tcgcaggaat gcaactaaga gacattactg aatcaaaaat ttattcagca
      421 atgcagaaaa tgacgaaccg gcgtcatgag gaaaactgga gactcagggc agaagcatgc
      481 agaaaaaaag ggaaacctgt tccagaatac acgccaaaac cagcgtccgt tgcaacgaag
      541 gctacgcatc tttcatttat aaaggcccta ctaagagccg cagagcgtga atggaaaatg
      601 ctggataagg caccaattat taaagtgcct caaccaaaga ataaacggat ccgctggctg
      661 gagccccatg aagcacaaag gctgattgat gaatgtccgg agccattaaa gtctgttgtt
      721 gaatttgcac tggcaacagg cttaagacgc tcgaacatca tcaaccttga atggcaacaa
      781 atagatatgc agcgccgggt ggcatggata aacccggaag agagtaaatc aaaccgcgca
      841 attggcgttg cgctgaatga tactgcatgt cgcgtattga aaaaacaaat cgggaatcat
      901 caccgttggg tatttgtgta caaggaaagc tgtaccaaac cagacggaac gaaagcgcca
      961 acggtcagga agatgcggta tgacgcaaac acagcctgga aagcggcgct gagacgagca
     1021 ggtattgatg atttcagatt tcacgacttg agacacacct gggcaagttg gctggttcaa
     1081 gccggagtcc cgttgtcagt gttacaggaa atgggaggct gggagtctat cgaaatggtt
     1141 cgtcgatatg ctcaccttgc acctaatcac cttaccgaac acgcacggca aatagactcg
     1201 atcctgaacc catcggtccc aaattcgtcc cagtcaaaaa ataaggaagg tactaatgat
     1261 gtgtaactta ttgatttaaa tggtgccgat aataggagtc gaacctacga ccttcgcatt
     1321 atgaatctgt agcaccaatc ataactatct gttttagcaa gcattaaccg cattcactaa
     1381 gctatagttg atgacacaaa cagaaagttg atgcatgatg ttgccatata tatgtcacaa
     1441 atacggcaca acgatcttca aacatatagc cacacattca cagaagagca caaagccttg
     1501 caatccagtg caaagcttgg tgtgtctccg ttttgtttca tcagttacac ataactatat
     1561 gatcattaac taaaagtcag tatacttgga gaaattttaa ttgcgtaata gagcatgcat
     1621 atgattgaaa ttaacagttt attattaata acatccgtga tattgatgtc gttattagct
     1681 gtggggttat ttgataaaat ttccccaata aatcttgttg agcacggcag gaataatcaa
     1741 atagatggaa tgcgggggtt cttagcaatt ttcgtgctta ttcatcacgc agcaatttgg
     1801 aatggatact tgtcatctgg agtatgggaa gcaccttcat caaatctgtt agcaaattta
     1861 ggccaagttg gtgtgtcatt cttttttatg attactggtt atctgttctt ttcaaagatt
     1921 atctctggag atcaggactg gacaaggctt tatgtatcaa gattactacg attaacccca
     1981 atgttcatag ttagcctatg tctcattttt atcattgtag gttttaagtc tggatggaga
     2041 atgcaggtat ccacagaaga gctttttgtg tcaataatga agtggttgcc attcactgcg
     2101 ctaggtatgc cgaacattaa tgacgtaaaa gattcattta ctatcaatgc cgctgtaaca
     2161 tggacacttg tatatgaatg gttcttttat ttttctcttc cggtaatttc cgcgctcata
     2221 aaaagaaaag tcagtattta catggttatg attagcgcaa tatcgctatt tgttttcatt
     2281 ttatttttca gtaaaataca catagcatca tttctttttg ggctcctggc atttttgcta
     2341 aataaatcaa agatagtcaa tggaattgcc aaagcaaaag taacaccaat tataataact
     2401 gcgataatgg tttttgaaat gacgtatttc aaaacaactt acgcaccgct accactgatt
     2461 ctttgtggaa taacatttat cattatcgca tcaggttgtg atttgtatgg aatattaaga
     2521 ttaaatataa ccagaaagct aggagaaact acctacagcg tttaccttct gcatgggata
     2581 ttcctttatt gcctaatgac gtggattatt cctaataatt acactgaaaa tacatttatc
     2641 atactggtaa gcacaactgc atttcttatt acgttcacat catgcctaac ttttaaatta
     2701 atagagacgc cattcatcaa attaacaaaa caaaccacaa ctttagtaaa agaattaata
     2761 cccacattaa caaacaacaa tcaataaaca ataaacataa gccgccctac atatatgtcc
     2821 aaatggggcg gcgaatgtat ttatattagt ctggtaatgc taaatgcaaa cgaactggag
     2881 attcctgccc cgctaagcgt attgcgcagg gtcaccctaa atgtatctac gccaatataa
     2941 gtaacatcac aactaactga atcaggcgca gatataggtg ttatctgcac aatataaccg
     3001 ctagcaactg ctgcgagcgg atgagaaaag ctaacatcat acacacctgt agaaaccacg
     3061 ttaaccgatg aaacaccagt agcaacgtcg atactaccat tggcgttaat ttttcccgag
     3121 cgtgtgactg acaatgcaga agccgatgtt accttactgg ttgtttctgt aaaaacacac
     3181 ccaatgtcaa ctacctcgca attagctcct gttatccagt atggtcgttc tgcacttggc
     3241 tgataatccc ctgcgctctg gaacgttgtg ccgttgagta tgactgtaac ttttccccca
     3301 cctgagcttg ttacttgaat attcgtgtgc gtgtatcttg cactgctcac gcgatgaaaa
     3361 tttccaccgt ttataaccac agttacaggg cgcgtcccag tattatctat agcaaggtct
     3421 gcccccccgg cattagcctc aaagtatggg ttgtttaata ccaaagcgca cgaaccgtta
     3481 aggccattta tagctaattg cgcaccacct gtactggaat ccccctgagt accatttcct
     3541 tcacaa
//
LOCUS       DAZXUL010000246         3493 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZXUL010000246 DAZXUL010000000
VERSION     DAZXUL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.247"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..311
                     /locus_tag="ACH3L1_004338"
     CDS             <1..311
                     /locus_tag="ACH3L1_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFT4078798.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="ACH3L1_004339"
     CDS             468..1217
                     /locus_tag="ACH3L1_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFT4078799.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="ACH3L1_004340"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="ACH3L1_004340"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFT4078800.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACH3L1_004341"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACH3L1_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFT4078801.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..>3493
                     /locus_tag="ACH3L1_004342"
     CDS             3361..>3493
                     /locus_tag="ACH3L1_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFT4078802.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     "
BASE COUNT          961 a          908 c          863 g          761 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aac
//
LOCUS       DAZXUL010000247         3471 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZXUL010000247 DAZXUL010000000
VERSION     DAZXUL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.248"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            56..295
                     /locus_tag="ACH3L1_004343"
                     /pseudo
     CDS             56..295
                     /locus_tag="ACH3L1_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
     gene            305..943
                     /locus_tag="ACH3L1_004344"
     CDS             305..943
                     /locus_tag="ACH3L1_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFT4078803.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            946..2109
                     /locus_tag="ACH3L1_004345"
     CDS             946..2109
                     /locus_tag="ACH3L1_004345"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFT4078804.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2193..>3471
                     /locus_tag="ACH3L1_004346"
     CDS             2193..>3471
                     /locus_tag="ACH3L1_004346"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFT4078805.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          779 a          815 c          997 g          880 t
ORIGIN      
        1 gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat tacctacatt
       61 gttaggttac gttatcccat tatccagtgc ggcaatcaat gcggtgagta ttccagacta
      121 tttcgcctgg ggcgggattg cactggtgat tcagttactc gtttttgctg gtgtcagact
      181 ttatatgccc gcattaagcg aaaaaattat taatcacaat accgcagcag gaatgtttat
      241 gggaaccgcc gcgctggctg gcggtatttt taacgcagct tgtatgacat ggtaatggac
      301 gatcatggcc agaaaacgca aatcaagaaa taacagtaaa attggtcacg gagcgatcag
      361 tcgcattggt agaccgaata atccttttga accgcgtcgc aatcgctacg cacaaaaata
      421 cttaacgttg gcgctaatgg gcggtgccgc ttttttcgta ttgaaaggtt gtagcgatag
      481 tagcgatgtc gataacgacg gcgacggaac attttacgcg acagtgcagg attgtattga
      541 tgacggtaat aatgccgaca tttgcgcgcg tggctggaac aacgccaaag cggcatttta
      601 tgccgatgtt ccaaagaata tgactcagca gaactgtcag tctaagtacg aaaattgcta
      661 ctacgacaat gttgaacaga gttggatccc ggtcgtttct ggatttttgt taagccgggt
      721 tattcgtaaa gatcgcgatg agccgttcgt ctataacagc ggcggatcct catttgcttc
      781 ccgccccgtc tggcgcaaca cttccggtga ttactcctgg cgcttcggtt ctggcaaaaa
      841 agagtcttac tcttcaggcg gctttaccac cagaaaagcg tcgaccgttt cgcgcggcgg
      901 ctatggtcgt tcttccagcg cccgtgggca ttggggaggc taatcatgct gagacacaac
      961 gttcctgtgc ggcgggatct ggaccagatc gccgctaaca acggtttcga ctttcatatc
     1021 atcgacaatg aaatctattg ggacgagagt cgggcttacc gttttactct gcgccagatt
     1081 gaagagcaga tcgaaaaacc gactgcggaa ctgcatcaga tgtgccttga ggtggtggat
     1141 cgcgcggtta aagacgaaga gatcctgacg caactggcga tcccgccgtt gtactgggat
     1201 gtgatcgccg aaagctggcg cgcacgcgat ccttcgctgt atggccggat ggattttgcc
     1261 tggtgtggca atgcgccggt gaagctgctg gagtacaacg ccgatacgcc gacctcgttg
     1321 tatgagtcgg cttatttcca gtggttgtgg ctggaggatg cccggcgcag cggcattatt
     1381 ccgcgtgatg ccgatcagta caacgccatt caggaacgcc tgatttcgcg ctttagtgag
     1441 ctttacagtc gggaactgtt ttatttttgc tgctgtcagg acaccgatga agacaggagt
     1501 accgtgctgt acttgcagga ctgcgcccag caggcagggc aggagtcgcg gtttatctac
     1561 attgaagatc tcggtttggg cgtcggcggc gtactgaccg atcttgatga taatgtcatc
     1621 cagcgtgcat ttaagctgta tccgctggag tggatgatgc gtgacgataa cggtccgctg
     1681 ctgcgcaagc gtcgcgagca atgggtggag ccgttatgga aaagtatctt gagtaataaa
     1741 gggctaatgc cgctgctttg gcgcttcttc cctggtcatc ctaaccttct tgcatcctgg
     1801 tttgagggtg aaaaatcgca gattgccgct ggcgaaagct atgtgcgtaa accgatctac
     1861 tcgcgcgaag gcggaaacgt caccattttc gacggtcaga ataacgtcgt tgaccacgct
     1921 gatggtgatt acgccgatga accgatgatc taccaggcgt ttcaacctct gccgcgattt
     1981 ggcgatagct acacactcat cggtagctgg attgtcgatg atgaagcgtg cggaatgggg
     2041 atccgtgaag ataacacact gatcaccaaa gacacctcac gtttcgttcc gcattacatt
     2101 gctggataag aatgttttag caatctcttt ctgtcatgaa tccatggcag tgaccatact
     2161 aatggtgact gccattgatg gagggagaca cagtgcactg gcaaactcac accattttta
     2221 atcaacctat accattaaat aacagcaact tatacctgtc tgatggcgcg ctttgcgaag
     2281 cggtaacgcg tgaaggtgct ggctgggata gcgattttct agccagtatt ggtcagcagt
     2341 taggaacggc tgaatccctt gaactggggc ggctggcgaa tgtaaacccg cctgaactgt
     2401 tgcgctacga tgcccagggg cggcgtctgg acgatgttcg tttccatccc gcatggcacc
     2461 tgctgatgca ggcgttatgt accaatcggg tgcacaatct tgcctgggaa gaagacgctc
     2521 gctccggcgc atttgtggcg cgcgcggcgc gttttatgtt acatgcgcag gttgaggctg
     2581 ggtcgttatg tccagtaacg atgacctttg ccgccacgcc attgttgtta cagatgttac
     2641 ccacgccgtt tcaggactgg accacgccgc tgctgagcga tcgctacgat tctcacttat
     2701 tgccaggtgg gcaaaaacgc ggtttgttga ttggcatggg aatgacggaa aagcagggcg
     2761 gttccgatgt catgagcaac accacccgtg cagagcgttt ggaagatggc tcttatcggc
     2821 tggtggggca taaatggttt ttctcggtgc cgcaaagcga tgcgcatctg gtgctggcgc
     2881 agaccgcggg cggtctgtcc tgcttttttg tgccgcgctt tttgcctgac gggcaacgca
     2941 acgcgattcg cctcgagcgg ctgaaagata agctgggtaa tcgctctaac gccagttgcg
     3001 aagtggagtt tcaggatgcc attggctggt tattggggca ggaaggggaa ggaattcgtc
     3061 tgatcctgaa aatggggggg atgacgcgtt ttgattgcgc cctgggtagc catgccatga
     3121 tgcgccgtgc attttcgctg gcgatttatc atgcacatca acgccatgtt tttggtaatc
     3181 cattgatcca acagcccctt atgcgtcatg tcttaagtcg catggcactt cagcttgaag
     3241 ggcaaacggc gttgctgttt cgtcttgcgc gagcgtggga ccggcgtgcc gatgccaaag
     3301 aagccctgtg ggcgcgttta tttacgcctg cggcaaaatt tgtgatctgc aaacgaggta
     3361 tgccgtttgt ggccgaagcg atggaggtgc tgggcggcat tggttattgc gaggagagcg
     3421 agctgccgcg gctttaccgg gagatgccgg taaacagtat ttgggaaggt t
//
LOCUS       DAZXUL010000248         3456 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZXUL010000248 DAZXUL010000000
VERSION     DAZXUL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.249"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(226..429)
                     /locus_tag="ACH3L1_004347"
     CDS             complement(226..429)
                     /locus_tag="ACH3L1_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078806.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="ACH3L1_004348"
     CDS             complement(431..586)
                     /locus_tag="ACH3L1_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFT4078807.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="ACH3L1_004349"
     CDS             complement(789..1196)
                     /locus_tag="ACH3L1_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4078808.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="ACH3L1_004350"
     CDS             1273..1500
                     /locus_tag="ACH3L1_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFT4078809.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="ACH3L1_004351"
     CDS             1484..1906
                     /locus_tag="ACH3L1_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFT4078810.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="ACH3L1_004352"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="ACH3L1_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="ACH3L1_004353"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="ACH3L1_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          764 c          940 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DAZXUL010000249         3379 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZXUL010000249 DAZXUL010000000
VERSION     DAZXUL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.250"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACH3L1_004354"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACH3L1_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFT4078811.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="ACH3L1_004355"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="ACH3L1_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFT4078812.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..>3379)
                     /gene="ynaI"
                     /locus_tag="ACH3L1_004356"
     CDS             complement(2466..>3379)
                     /gene="ynaI"
                     /locus_tag="ACH3L1_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFT4078813.1"
                     /translation="LFHAVQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTS
                     LIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSL
                     SGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIG
                     WRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAV
                     REMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDI
                     VQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT          957 a          730 c          789 g          903 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaa
//
LOCUS       DAZXUL010000250         3364 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZXUL010000250 DAZXUL010000000
VERSION     DAZXUL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.251"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..247
                     /locus_tag="ACH3L1_004357"
     CDS             <1..247
                     /locus_tag="ACH3L1_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078814.1"
                     /translation="TNGSVRYCQYTSQRHPDKRFIFNGNEIWKRGESTTEYTRAHQRT
                     IRRLRRLIARKQYTEKWFRKYDTWSAGYYELMATKPF"
     gene            308..496
                     /locus_tag="ACH3L1_004358"
     CDS             308..496
                     /locus_tag="ACH3L1_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078815.1"
                     /translation="MNIYFRIVISLAIIACIYGLIVPALISMKDTVAVISGFALACLT
                     PPCIYAIYKGLSFSKDKR"
     gene            493..1320
                     /locus_tag="ACH3L1_004359"
     CDS             493..1320
                     /locus_tag="ACH3L1_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFT4078816.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1364..2113
                     /locus_tag="ACH3L1_004360"
     CDS             1364..2113
                     /locus_tag="ACH3L1_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFT4078817.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2110..2679
                     /locus_tag="ACH3L1_004361"
     CDS             2110..2679
                     /locus_tag="ACH3L1_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078818.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2676..2798
                     /locus_tag="ACH3L1_004362"
     CDS             2676..2798
                     /locus_tag="ACH3L1_004362"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078819.1"
                     /translation="MKIEYQDAAGGESMSWPDAIVTLGMVFAVAFVVYSICRWG"
     gene            2803..3045
                     /locus_tag="ACH3L1_004363"
     CDS             2803..3045
                     /locus_tag="ACH3L1_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFT4078820.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3049..3195
                     /locus_tag="ACH3L1_004364"
     CDS             3049..3195
                     /locus_tag="ACH3L1_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078821.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3204..>3364
                     /gene="xisR"
                     /locus_tag="ACH3L1_004365"
     CDS             3204..>3364
                     /gene="xisR"
                     /locus_tag="ACH3L1_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFT4078822.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          940 a          808 c          861 g          755 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcacaa cgccatcctg acaagcgttt
       61 tatcttcaac gggaatgaga tatggaaacg tggagaatca accacagagt atacccgcgc
      121 acaccagcga acaatcagac gactgcgcag actcatcgcc aggaaacagt atacagaaaa
      181 atggttcaga aaatatgaca catggagcgc cggatattac gaactgatgg caacaaaacc
      241 attctgacgt aaacgaaatt aaccatgacg caattaaata aggcaagccg aatacatcag
      301 gaggaccatg aacatttatt tcagaatagt tatatcactg gcaattatcg catgtattta
      361 cggattaatc gttccggccc tcatatcaat gaaggatacg gtagcagtga tttctggctt
      421 tgctctggcg tgtctgaccc cgccctgcat ttatgccatt tataagggtc tttctttttc
      481 taaggataaa agatgaaaaa agttattcct gctttagccc ttgttctgct gaccaccgga
      541 cttgtcggtt gcgatcgcgt tgagcctggt aatgttggca tcaaggtaaa caaactgggc
      601 gacgataaag gcgtcggaga agtggtcggt gttgggcgct actggacggg atggaacact
      661 gaggtttaca tcttcccgac cttcaaacaa atgaagacct acgatgagcc attcagcttc
      721 cagatgagtg acggtacaac catcggctat cacatcggcg tggcctacaa agttgatcca
      781 tccaaagtta ccacagtctt tcagacctac cgcaaaggcg tggacgacat taccgacacc
      841 gacctgcgcc agaagatagc cgacgcactc aaccgactgg ccagcaaaat gaccaccgac
      901 aaatttatcg acggcggcaa gtctgagctg ctggatgcag ctcttaaaga cattcaggaa
      961 gaaatgacgc ccatcggtat tcaggtaatg agcctctcat atgtgggtaa gccggagtac
     1021 ccgcctactg ttatcgacag cattaatgcc aaagtcacgg cgaaccagaa aaccctgcaa
     1081 tgcgagcagg aagtaaaaca gcgcgaagcg gaagccaaca tgttgcgcgc ggaagctgcc
     1141 ggacaggcag atgcgattcg cacaaaagcc caggccgaag ccgatgctat tcgtttacgc
     1201 ggtgaagctc tgcgccagaa cccaaacgtt atggagctgg aagccatcaa caagtggaac
     1261 ggtacactgc cgcaatacat gaccagcggt gccaacacac catttattca ggttaagtaa
     1321 tacatatgcc cggtattaca cgccgggctg tctggagata aaaatgaata ctgtaaccat
     1381 caacaacaaa cagtttccgg taatcgaata tcgcggtcag cgtgttgtga cgctggcgat
     1441 gattgatgaa gtccaccagc gaccggaagg aaccgcgcgt gcagcattca accgaaaccg
     1501 tgagcatttc atcagcggtg tggattatgc cgaattaggt gcggacgtaa tacgtacgga
     1561 cctcccggaa ggaacattct ctaaatttgc accatcgggg attgtacttt ttgaatcagg
     1621 ttacctgatg ttgacgaagc cattcaatga cgcccttgcc tggcaggttc agcgcgaact
     1681 ggttaacagc tacttccgca ctcgcacacc gctaacggaa atcgagatga tcgctgcgat
     1741 agccgccgac gctgttcgtc aacagaagcg cctgaatcat gtggaagaac agctcgaaac
     1801 agtcacagaa gcggtggaaa ccatcaaacg cggaaatatg cgcgccggat atgtcggtta
     1861 ccgccaggtg gtagccaaaa gtggaatgag tgacaccaag tgtcggaatc tggtgaacgc
     1921 atatcaaatt cctacagaca cccacgaatt catgactccg gacggtctgt tgtctcgcag
     1981 ggctatcgtc gagcttgagc catttatggc ggcgttccac caaatgatgt cagaggctga
     2041 cccacgcggc acacgctggt atcacccgaa gatggggctt ttccaggtga tcggatggga
     2101 ggataaagca tgatcatcca gtcaaaactt attcgcgccg ctctggtgtg cgctgctaaa
     2161 aacgacgttc gttattacct gaacggtctt cacatcacgc cgaaacatat tgaggcaacc
     2221 aatggttccg tggcactgcg catggctcac ggcatccgga cgaagaaaaa catcattgtc
     2281 cagttcgaag ggggcgtccc ggccaaagcc gaaacgacag agctgatttt tagtaaagag
     2341 ccgatcgctg ttcatcgcga ccagtttcag cgacgactgt ccattaccgg cattaaactg
     2401 gtggacggtt gttttccgga tttggatcgc atcattccga aaaagtttga ccgctgtact
     2461 cacccggtgc tccaggcggg ttacctgagt tatccagaga agatgtttgg tcgtgagcgt
     2521 aaatttattc ccgtccagtt acgtccctcc ggtgacgggc aagcggtcag aattcagttt
     2581 gattccatca tcaactcaat gtatggcaat cctgaatttg ttgtgatgcc ttgtcgtgat
     2641 catggcgatt tcaacgtggc tcaggagcat ccggaatgaa aatcgaatac caggacgccg
     2701 caggaggtga atcaatgagc tggcctgatg caatcgtaac tctggggatg gtattcgcag
     2761 tagcgtttgt tgtgtactcg atttgtcgat ggggataacc acatgttcgc tttgattcaa
     2821 cgcgggcaga tatacacaga cagagccgga tacccagtag aaattactcg cagtactgag
     2881 cactcagtgt tctttcggag gatggacgga cgtaccgggc gagtacgcat cggtgaattc
     2941 agtagcctgt tcgagcatat tgaccacctg gaatatcaca agattctggc tgaaacagaa
     3001 caagaaaagc acctgaagaa attacgcgca atgcaaagga agtaaaaaat gaataaagcg
     3061 tttgagctat gggtccacca gcgttacggc aaccactacg acctgacgcg agatgttgac
     3121 ggtttctact gtcgtgaaat tgtgaaacga atgtttgagg tctggtgcca ctgccgtggg
     3181 ctgagtgttg tgtgaggtaa tgcatgggca atgtgattca actggctccc aatgaatggg
     3241 tttgtgaaag cgttctaatc gcaattaccg ggctcaaacc aggcacaatt cttcggaccc
     3301 ggaaagaatg ctggatggtt ggaagagagt atattcacgt atcaccagac ggtaatccaa
     3361 agcc
//
LOCUS       DAZXUL010000251         3347 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZXUL010000251 DAZXUL010000000
VERSION     DAZXUL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.252"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            18..896
                     /locus_tag="ACH3L1_004366"
     CDS             18..896
                     /locus_tag="ACH3L1_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFT4078823.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            893..2284
                     /locus_tag="ACH3L1_004367"
     CDS             893..2284
                     /locus_tag="ACH3L1_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFT4078824.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2281..2538
                     /locus_tag="ACH3L1_004368"
     CDS             2281..2538
                     /locus_tag="ACH3L1_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078825.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2633..2833
                     /locus_tag="ACH3L1_004369"
     CDS             2633..2833
                     /locus_tag="ACH3L1_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFT4078826.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2826..3206
                     /locus_tag="ACH3L1_004370"
     CDS             2826..3206
                     /locus_tag="ACH3L1_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFT4078827.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          725 c          969 g          768 t
ORIGIN      
        1 attctgaggt gacagcgatg atgacgttta acctgcgtga acaacaaaaa agactacagg
       61 cgcgaatgga tgagttacgg gcagagattg catttgctca gaagggcgaa aagccatggc
      121 cttatcgttc ctgcctgatg cgtgaaggtc gcggatattg cgaaaaacat ggcgaatatc
      181 acacgcatat actggtgtgg agcgatcgta atggcgagga cagagaagaa atttcatgct
      241 gccctgactg cttaatcgct gaggccaacg atttgaccat ggagctgtcg tccatcaagg
      301 cggaagagct gactgataac gccggaattg ccctgcgttt tcgggactgc gagtttgata
      361 attatctgga ggttaatcct ggcgcagcca gaaatcttgc ggcctgtcgc cgctatgcgg
      421 agaactggcc agatatgctg gagaacggta ccagtcttgt tatgaccggc agttgtggta
      481 ccggaaaaaa tcatctggct gtggctatgg caaaacacat catccgtaac tatctggcca
      541 gtgtggagat caccgacgtg atgcgcctta cccgtgctgt gaaaaattgc tggcggaatg
      601 acagcgaaaa aacagcggat gaagttattg agcgttatgc gtcaatggat ttgctgatca
      661 tcgacgaagt tggcgttcag tttggcagcg cggctgaaat ggctattttg caggaaatta
      721 tcaacgccag gtacgaaagt atcttgccca ccattctgat cagtaacctc tcaccggaag
      781 agttgtgggc gttcatcagt ccccgaattg ccgacaggat cacagacggg ggacgcaact
      841 ggttgtcgtt taactggccc agttaccgtt ctcgtatcag aggtgtggct gcatgacaac
      901 tccagtatgg cgtaacgatg accttgaagg cgctgtcatc ggcgcgttct ttctgcgcgg
      961 agcagatcct gaagtgatgg atattctggc cacactgcca gcggacgttt tttctgtacg
     1021 agcgtatcag gatatctata caggcatctg cagacaggcc cgtgtttcag gagtgattga
     1081 ccccgtgctg ttgtgtaatg agatgccgga acttgccccg gtgattactg ataccgggcg
     1141 taaaacctgg gtgaagtctt cactggagca ctatgttgca gcgttgcggc gcaatgccgc
     1201 actgcgcgat gcagaaaaaa cactgaatga ggcgctgcag aaattacgtg atgcgcatac
     1261 ctgtgaagca gctgaagatg ccctgaagga tgcgcagaac atgatggtca cactgtcgac
     1321 aggaaagggc gtcattcagc cggtacatat tgatgatgtg cttccggaag tggttgagcg
     1381 tgttgaatgc cggaatcagg ggctggagaa atccaggacg ttgatgaccg gtattgatga
     1441 actggacgca aaaacaggcg gtatggagcc cggagacctg gtattcattg ccgcccgtcc
     1501 ttcgatgggg aaaaccgaac ttgcgctgga catcatcgac aaggtgactg agcaggggca
     1561 tggtgtgctt ctgttcacca tggagatggc gaacatccag attggtgaac gtatggtgtc
     1621 tgcggctgga gggatgccag tatcacgcct gaaatctgtg gctcactttg aagatgaaga
     1681 ctggacgcgt ttctcacaag gggtggggcg gatgaccggg cgcaatatct ggatggtgga
     1741 ccaggcgaac ctggccattg atgagatatg cgcaacaacg aaacaccacc tgattaaata
     1801 tccggaaacg gcgctggtgg tggttgatta tctcgggctg ataaaaaccc gaaccacggg
     1861 gcgtcatgac cttgccgtgg gtgaaatctc aaaggggctt aaaggcctgg caaaatccgg
     1921 tggttttccg ttgattgcgc tgagccagct ctcccgcggt gtggagtcca gacccaataa
     1981 acgtcccatg aactcagacc tgaaaaattc cggagaaata gaggcggatg cagacatcat
     2041 tctgatgctt tacagggatg aagtgtacaa cccggatacg caggccaggg gcatcgcaga
     2101 aatcaatatc acgaaacaac gtaacggttc tctgggaacg atttaccggc gtttttataa
     2161 cggacatttt ctgcctgtgg accaggaaag tgcacgggtg ctttccactc ccatgaagcc
     2221 cggcaatccg cgcagataca gtaataaacg aactgacagc agtaagatgg agcgtttctt
     2281 ttgaacaacc agacaatgac ttttacccct gaacaattgc gtaaacacgc gcaggaaatg
     2341 ctgcgtcatg ctgaacaact cgaaaaaacg ggcataacaa aagatgctat ccgtaaagat
     2401 atggtacccg cgcttcggga actgatgcag gcgaagcatc gcgcacagaa agcagtagat
     2461 gagctggtgg actgtgtggc agagctggaa accagagtcg gaaagtttga aaaactggtg
     2521 caggaggcgc tgcgctgatg cgccatgagt ttgttttgcc ctggcttgtg cgcgatcatt
     2581 atgtccctgg tggaaagccc gggataaaaa tcaccgaacc ggagagtatt tgatgagcgt
     2641 caaaattcaa acgataccgg aactgcttat ccagacacga ggtaatatga ccgaagtttc
     2701 acgaatgctg aactgtaatc gcgccacggt aagaaaatat gctgaagata aagagggcaa
     2761 agggcacgcc attgttgatg gtgttctgat tgttcatcgt ggatgggaca ggggtaaaga
     2821 cagtgatgcg tgatattcag caggttcttg agcgctgggg cgcgtgggtg gctaataatc
     2881 atgaggatgt gacctggtcg tccattgctg ccggttttaa gggattaatc ccttcaaaag
     2941 taaaatcccg cccgcaatgc tgtgacgatg acgcgatggt tatatgtggg tgtatggccc
     3001 gccttaaccg gaacaacaac gatctacatg acttgctggt tgactactac gtgtttggga
     3061 tgacattcat gacgctggcg cggaagcatg gatgctcaga tggttatata ggaaagaagc
     3121 tacaaaaagc cgagggggtg gtggaaggaa tgttgatgat gttgggtgtc cggttagaaa
     3181 tggacaaata tattgaacat ccctgatggt aaggggcgac tttctagaaa atattttacg
     3241 tacgtaaaaa acgggatatt ctgttaagag tggttgcttc gccacacagc ttaaacccgc
     3301 ctccgggcgg gttttttatg cctgaaaaac ggtacaggac gttaaac
//
LOCUS       DAZXUL010000252         3300 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZXUL010000252 DAZXUL010000000
VERSION     DAZXUL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.253"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(304..447)
                     /locus_tag="ACH3L1_004371"
                     /pseudo
     CDS             complement(304..447)
                     /locus_tag="ACH3L1_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            418..702
                     /locus_tag="ACH3L1_004372"
     CDS             418..702
                     /locus_tag="ACH3L1_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078828.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            complement(704..1276)
                     /locus_tag="ACH3L1_004373"
     CDS             complement(704..1276)
                     /locus_tag="ACH3L1_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078829.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(1362..1568)
                     /locus_tag="ACH3L1_004374"
     CDS             complement(1362..1568)
                     /locus_tag="ACH3L1_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFT4078830.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(1954..2745)
                     /locus_tag="ACH3L1_004375"
     CDS             complement(1954..2745)
                     /locus_tag="ACH3L1_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078831.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
BASE COUNT          914 a          680 c          754 g          952 t
ORIGIN      
        1 ttatatgaat tttatcatct tactctgaat gttcatttca tcagtgtgac attccattgt
       61 ttactgataa agttagccga taaataatat tgttgagatt attgaaacgg cagttttcgc
      121 cgattcagtg caatggaaga gatgttatat catctattta agcaataagt ttaatacaag
      181 tttcagaaga ggtgttggtg agctgatgac tgattttcag agggatatgg aaaagtgtat
      241 agaatcccat aaaaaatcga tattgttcag ttgacgtata tttctggagg atgactccag
      301 tcatcagaac atatgttttt cgatgaatga cccaataatt ttttcgtgaa tttccacagc
      361 acgtgaaaag taaatcgttt ttcggtccag tcgtttgatg cgggaacgga gtgtcagatg
      421 gtggtgctca atacgccggg taaatatcaa tacgatttga ggaaccattt gctggttaat
      481 ctctttccga tagcattgga tgcagaaata actacatcca atgtaaataa gcatcaattg
      541 cacagaagca atatacctgc ggccctctcc ggaaattccg gtatcactga ctacgtaccg
      601 ttttcagcgg aacgaaatgc ggcgatggtc atttggcgga cagccataga tagccgtccg
      661 gctgtgtgta acgatgagga aggaaccccg ggaacgttat gaatcagcag gcgtataaca
      721 ggtactcgtc aatcatcagt tccagctcgc gcttttcccg tttgcgcgcc acagcccagt
      781 tttcaagctc tgcatgtgat ttatgcttta actcttcagt ccagggctgc atggagtggg
      841 cacacatcag atcataccag cacagccata tcagcgtgtg gtagagatca cgatcgtcga
      901 aacgccggag caggctgttc agcctgtttg ccagccggtt ttcacccaga cgatgtagct
      961 cactgagtaa aacgccgccc agattaaaat gtgtaagcag catgatttca tgggtctgaa
     1021 tgctggtcat taacccttcc tgaatggcag gaatgaacgg tggtgttccc gggttgacga
     1081 cctgatagag tccctggggg gccaccgaaa tgacggagcc tttttccggc agaaaaaagt
     1141 gtgccagggt cagacggtga taaccatgaa gatgcggaga ccccattcca tgagcgacga
     1201 taagcctcgt cacgcttgtc atccatttct gatgctggat gagcagcagg tcacacctgt
     1261 tttcaagttg ttgcataata ttctctcctt tagcgcgcag caaaatgcct ccgggggccg
     1321 gatcatgcct ttgctgcggt ggaacggata acggtcagga attacggacg ggactgtgca
     1381 atacgcgcct gcagccaggc ttccacttcg cttttcagcc agcgggagct acggcccagt
     1441 ttgatggggg gcggaaaggc tccatgcttg atgagtttat aaaaccactt atcggtcagg
     1501 ccggtcagct gagtgataaa cgacatgtcg accatctggt catccatcag tgaaactggg
     1561 gtattcatga gaatgttcct ccggatgttg ttaatctcaa ccggcgttgg tgatgaaaac
     1621 gacgcaccac cttcaccggc ataacaaccg gggagaaacg taaaaaatga gtaggggaat
     1681 attcctgcca ggagagaaac tgttgtgtac aggcaataca ggttgtctgt tttgtcagtg
     1741 aatccggtat aaacggaaga tgattttgtg ttgccggaat atgggatggc gttttgccgt
     1801 gacgggatca gtattttcag tgatgattat tactgcacag gggagtatcg gagataaacc
     1861 acgccctgta cctgccagag cggtggtttg tatttactga acggaagata aagcagaggt
     1921 aaacactgtc gcggtgaagg tagaaggctg aaattattct ctgtaattac aaacaacagt
     1981 gatcttatcc ggttttttta ccgttacggg tttcagaggg tcaattttat atacaggggt
     2041 aatgagcacg ccggaattta tggaaaaaag agggatccag caataaacgt ccggatatgt
     2101 ctgtgtctcc ggaatacctt ttgcgccaga gaccatcaga tctgtaacgc tcacaaaaaa
     2161 tgactgccgc aaatcaaaaa agtactcgtt atatatcaca tcctttgcca gcaggatgac
     2221 tgcattacca acagccgctt ttacaggtat tttatcagat ggattatcgt gattgtatat
     2281 tgttctttcg atataaataa aacaggcata tttcgtcttc tccccggata gctgcatctg
     2341 gtattcgcaa atgtctttga tacaccgact ttcaaccctt ccaatcatct tatcccggcg
     2401 atagtgtctt ttatgtttca gcgcgtacag aacaccattt gaactgtcaa actccaggat
     2461 tcttctggtg aaaagtttgt tttcccagat ttccagacaa tacttctctg cactgacgac
     2521 attgcgtttg cgaggaagat cccgttgata cactccattt aataagcgtg actgcagttc
     2581 gcctgcaagt tgataataac agttaatatc attaaggaca ggtgctgtgg cgctgagtaa
     2641 tgcgttgatt accttagagc gcgaaagttt gtgggtatga cagtactcat ccacaattcg
     2701 cagcgtctca tcactggtca tactgaacgt tatacgtgtt gtcatgatga agttttctgt
     2761 taatcatatt ggttgagatg cataacattt cataaaaaaa cctgttaatt catgcaatta
     2821 attttttaat gaattttatc ctcaagtgat ggaaagcagt gttactgcca ctgtactcag
     2881 tataactcta tcacacagaa tcacagaaaa tgaagcattg ctcaggggga cactcatacg
     2941 agttctctga actgtgataa ccagtatttt tcattttggg tgatttgttt atcgttacag
     3001 ctacaggtag ttcatgtacc ataaaaaaat aattaaggtt aattagtatt atttacagaa
     3061 gtatggtcat gtaaatgatc ctgtgattat accttgtaaa tacaccttat aacccaatcc
     3121 tgtagacgga tctctcataa agatatactc atagaagtat ctgtgcagtg atattctctc
     3181 tagtagtact tgtaactctg tccttacccc tctctgctgt aatgtagtcc cgcaacacct
     3241 gaatataaat ctgtaatgta cacactgact gtaatgttct ttgttaagta atgtctgtta
//
LOCUS       DAZXUL010000253         3187 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZXUL010000253 DAZXUL010000000
VERSION     DAZXUL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.254"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            168..1271
                     /gene="proB"
                     /locus_tag="ACH3L1_004376"
     CDS             168..1271
                     /gene="proB"
                     /locus_tag="ACH3L1_004376"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFT4078832.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1283..2536
                     /gene="proA"
                     /locus_tag="ACH3L1_004377"
     CDS             1283..2536
                     /gene="proA"
                     /locus_tag="ACH3L1_004377"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFT4078833.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2651..2726
                     /locus_tag="ACH3L1_004378"
     tRNA            2651..2726
                     /locus_tag="ACH3L1_004378"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2684..2686,aa:Thr,seq:cgt)
     gene            complement(2741..>3187)
                     /locus_tag="ACH3L1_004379"
     CDS             complement(2741..>3187)
                     /locus_tag="ACH3L1_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4078834.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
BASE COUNT          752 a          807 c          877 g          751 t
ORIGIN      
        1 tggtaattta ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc
       61 gcgcaacaaa acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa
      121 aacgttcgtt tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc
      181 agacgctggt ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc
      241 gtgcccatat cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga
      301 ttgttattgt gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac
      361 tgccagcgac tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc
      421 aactgtggga acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc
      481 gtgctgatat ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc
      541 tcgataacaa tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta
      601 aggtcggcga taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac
      661 tgttgctgct gaccgatcaa aaaggtttgt ataccgctga tccgcgcaga aatccgcagg
      721 cagaactgat taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca
      781 gcgtttcagg cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt
      841 gccgtgcggg tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg
      901 tgatggaagg catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc
      961 gtaaacgctg gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa
     1021 ctgccgccat tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg
     1081 gcaatttctc gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc
     1141 acggtgtcag tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag
     1201 aaattgatgc aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga
     1261 ttacccgtta aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc
     1321 gtataaatta gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga
     1381 tgaactggaa gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc
     1441 gcgagccaat ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct
     1501 gaaaggcatt gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt
     1561 aatcgatggc ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg
     1621 ggttattggc gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg
     1681 cctgaaaacc ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc
     1741 ggcaacggtg gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt
     1801 gcaggcgatt gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata
     1861 catcgacatg ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc
     1921 gacaatcccg gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagtgc
     1981 agagatcgct gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg
     2041 taatacggtt gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt
     2101 aagcaaacaa atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca
     2161 gttgcaggca ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt
     2221 tctgtcatta gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat
     2281 tcgtgaacac ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca
     2341 gcgttttgtt aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac
     2401 cgacggcggc cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc
     2461 gcgtggccca atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta
     2521 caccattcgt gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc
     2581 attgacgcat cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg
     2641 tcctttcagg gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt
     2701 aggttcgact cctattatcg gcaccattta aatcaataag ttacacatca ttagtacctt
     2761 ccttatttct tgactgggac aaatttggga ccgatgggtt cagaatcaag tctatttgcc
     2821 gtgcgtgttc ggtaaggtga ttaggtgcga ggtgagcata tcgacgaacc atttcgatag
     2881 actcccagcc tcccatttcc tgtaacactg ataacgggac tccggcttga accagccaac
     2941 ttgcccaggt gtgtctcaag tcgtgaaatc tgaaatcatc aataccagcc cgtctcagcg
     3001 ccgctttcca ggctgtgttt gcgtcatacc gcatcttcct tactgttggc gctttcgttc
     3061 cgtctggttt ggtacagctt tccttgtaca caaataccca acggtgatga tttccgattt
     3121 gttttttcaa tacgcgacat gcagtatcat tcagcgcaac gccaattgcg cggtttgatt
     3181 tactctc
//
LOCUS       DAZXUL010000254         3155 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZXUL010000254 DAZXUL010000000
VERSION     DAZXUL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.255"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..619)
                     /gene="dsbA"
                     /locus_tag="ACH3L1_004380"
     CDS             complement(<1..619)
                     /gene="dsbA"
                     /locus_tag="ACH3L1_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFT4078835.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSE"
     gene            complement(636..1622)
                     /gene="srkA"
                     /locus_tag="ACH3L1_004381"
     CDS             complement(636..1622)
                     /gene="srkA"
                     /locus_tag="ACH3L1_004381"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFT4078836.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1699..1968)
                     /locus_tag="ACH3L1_004382"
     CDS             complement(1699..1968)
                     /locus_tag="ACH3L1_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFT4078837.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2038..2622
                     /gene="mobA"
                     /locus_tag="ACH3L1_004383"
     CDS             2038..2622
                     /gene="mobA"
                     /locus_tag="ACH3L1_004383"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFT4078838.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2604..3131
                     /gene="mobB"
                     /locus_tag="ACH3L1_004384"
     CDS             2604..3131
                     /gene="mobB"
                     /locus_tag="ACH3L1_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFT4078839.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          828 a          770 c          761 g          796 t
ORIGIN      
        1 cctcggacag atatttcacc gtatcagcat actgctgaac aaaaacatcc atattgctgg
       61 tatccatacc ctgcggattc agctgatatt taccgttaac aaacatcgcc ggaacaccac
      121 gcaattgcac atcagctgcg gctttttcct gctgagcgac cagagatttc accacgaagc
      181 tgttccacgc cgcgtcgtac tcttcacctt taatacctgc attgataaat acatcgcgga
      241 tatcagatgc tgaacgaatg gtctgggttt tctgtacgcc ttcaaacagc ggaacagtca
      301 ctttgtcttc cacgcccagc gccatcgcca cagcccatgc ctgagtcaga tctttgccca
      361 ggtcaccacc catgaagttg acgtggtatt tagtcatctt cacgccttcc ggcagttttt
      421 tcttcacatt atcagaaata tgcagaactt cttcaaactg atagcagtgc gggcagaaga
      481 aagagaaaaa ctccagcact tgcggcgcgc cagctaccgg tttttccagg gtagtgtact
      541 gtttaccatc ttcatactgc gccgccgatg cgctaaacgc taaaactaaa ccagccagcg
      601 ccagccaaat ctttttcatg atctactctc tccgattaat acataggtgt taattgcaaa
      661 gggggttctt gtagaacttt tgcctgttct ataaaagtcg ccgtctgtcg tagccagtaa
      721 tcttccccgg ttaaccacgg gaaatttttc gggaacgcgg gatcagccca atgccgcatt
      781 agccaggcaa gataataaac caaacgcatg gcgcgtaaag gttcaatcag tccgatttca
      841 gcggtgtcga actcgctaaa ttcttcataa gcttcaataa tagtttccag ttgcatccgc
      901 tgctgggctt tatcgccatt gagcaacatc cacaaatcct gaatagctgg accattacgt
      961 gcatcatcca gatcaacaaa cattggacca tcgcgccaga gaatattccc ggcgtggcag
     1021 tctccatgta gccgcagaac ggtgaaatct tcccgccagt gtgctgtaac ggcggcgatc
     1081 agctcatctg tcgctttcag aaatgccgct ttcaacccgg aaggtatcag tgtagcgtcc
     1141 tcaaacagct tgcgaggctc aatgagatat tcattcagac cgatggtcgg gcgatgaata
     1201 aaaagctgtt tgcgccccgt ctggtgcata cgccctaaat aacgcccaac cgcttccatc
     1261 tgatcgatat tatcagcttc gaactggcga ccaccgacgc ttggaaaaac agcgaaataa
     1321 aatccctgat gattcaataa agtctgaccg ttaaaggcca caggtgctgc gaccggaact
     1381 tcatcattta ccagctgcaa cgcaaattga tgttcttcga ggatttgatc ggctgtccaa
     1441 cgttcagggc gataaaattt gacgacaaaa cgtcgacgct cttcgtcctg aaattgatag
     1501 acacggtttt catagctgtt aagcggggta agaccggaat ccacccggat cccttgctta
     1561 aacagagcgt ccatgatggt atccgggtgt agtgtctgga aagtaaaagc gctgttgttc
     1621 atcccatcat ccggaaaaca ttacgaatga tgaaggatat catttcacga tgattttggt
     1681 ggcgccgctt acaagctttt actctttaat tacgccgcga gcgcgtaaca gcgccgtttt
     1741 gaagtcttct tcataatctt tctgcaaacc cgggatcacc gcatctttcg cggaatcacg
     1801 cattttcagg tgatagatca gaatgtcatc cgtcaaatcc gccagttcgc cgtcaaaacc
     1861 tgactctttc gccagtttct gcaaaaattg caacaagtta aagtctggct ctttttgcca
     1921 ggctggctgg aggagttcaa taacttcatt cagacgttta catttcatag taatgctcct
     1981 tactcttgag acagacacgt tagcagggtc aatcccacaa taaaagaggc gatatcggtg
     2041 aatctaatga cgacgataac aggcgttgtg ctggcaggcg gtaaagccag acgaatgggc
     2101 ggcgtagata aaggattgct ggacttaaat ggcaaaccgc tgtggcaaca tgtcgctgac
     2161 gcgcttaaga cgcagctctc tcacgtcgtg gttaatgcta atcgtcatca ggaaatctat
     2221 caggcaggcg gtctgaaagt gattgaagat tcactggcgg attacccagg ccctctggca
     2281 ggaatgcttt cagtaatgca gcaggaagct ggtgagtggt ttttgttttg tccgtgcgat
     2341 acgccttaca ttccccatga tttagccgcc cggcttaatc atcagcgcaa agatgcgcct
     2401 gtcgtgtggg tccatgacgg tgaacgcgat cacccgacta ttgctctggt aaaccgcgct
     2461 attgagcctt tattactgga atatctgcaa gcaggagaac gccgggtaat ggcatttatg
     2521 cgtctggctg gcggtcatgc ggttgatttc agcgatcata aagatgcatt tgttaacgtg
     2581 aatacgccag aggagcttgc ccgatggcag gaaaaacgat gataccgtta ctcgcctttg
     2641 ccgcgtggag tggtaccgga aaaactacgc ttttgaaaaa actgatcccg gcattatgcg
     2701 ccagagggat ccgtccgggg ctgattaagc atacgcacca tgatatggat gttgataagc
     2761 caggcaaaga tagctatgag ctgcgcaagg ctggcgcggt acaaaccatc gttgccagcc
     2821 agcaacgatg ggccttgatg acggaaacac cagacgaaga agagctggat ctacattttc
     2881 tcgcaagtcg aatggatacc tcaaagctgg atttgattct ggtcgaaggg tttaagcatg
     2941 aagagatcgc aaagattgtg ctgtttcgcg atggagccgg acatcgacct gaagaattag
     3001 tgatagacag gcatgttatt gctgtagcca gtgatgtgcc gcttaatctt gatgtcgcat
     3061 tactggatat taatgatgtt gaggggctgg ctgattttgt ggtggagtgg atgcaaaagc
     3121 agaacggata agcaagagta ggctggatca gacgc
//
LOCUS       DAZXUL010000255         3112 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZXUL010000255 DAZXUL010000000
VERSION     DAZXUL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.256"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACH3L1_004385"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACH3L1_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFT4078840.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="ACH3L1_004386"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="ACH3L1_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACH3L1_004387"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACH3L1_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFT4078841.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACH3L1_004388"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACH3L1_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFT4078842.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="ACH3L1_004389"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="ACH3L1_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAZXUL010000256         3097 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZXUL010000256 DAZXUL010000000
VERSION     DAZXUL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.257"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(104..1813)
                     /locus_tag="ACH3L1_004390"
     CDS             complement(104..1813)
                     /locus_tag="ACH3L1_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFT4078843.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2245..2964)
                     /locus_tag="ACH3L1_004391"
     CDS             complement(2245..2964)
                     /locus_tag="ACH3L1_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFT4078844.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          953 a          588 c          519 g         1037 t
ORIGIN      
        1 gccaattgag gtggttgcct gaaagttggc agttacccgg tcactacacc ttgttggagt
       61 gaacatatca aaaacaggta attacacaat ttaaattaca gtctcataaa aaatgattaa
      121 atctatttat tatttcaata aacgtttttt tcatcttctt tgaaaattgt tctgctatag
      181 tatccggttc cagattgttc tcttttatta ttctacaata ctctaatatt gtttctttgt
      241 ctctatttac tacagataca aatacaccag gaataccatc tttattagtt gcggacagca
      301 tttcgaccaa tcgtcttgtg gtaaaactat agttttttag aatattacaa aaggcacgta
      361 ttgtttcggt atggccatta taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt
      421 ttatccataa taattcatct atatattcct ggtgaggggg gagtattttt agtatatctc
      481 catacgccat aatggccttt gtgtgcccat tttgaagtgc cataaacaac ccatgcttta
      541 ctttcaataa ttctttaatt tgttcactac taagcatttt cttattaagc agacttccga
      601 acatacgaat actatctgca tgtccattct gcaatgcaaa aaataaccca ggtaaatcat
      661 ggctgtcttt cgcagctaat agatataatc tcttatcgga agttaacggg gttttaaaga
      721 ctatttcccc ataggctttt attgtgtcta catgtccgtt cataaaagac atgcataaac
      781 ccggcacatt gtttgcccct accgcagcaa gcaatgtaag tattgtttct tgactaagat
      841 tgcacatatt taatatctta ccatatactt gaataatgtc tgaatatccg ttttgaagtg
      901 ctatataaaa tgcaggagta ccatcagagc ttctcgtggc cagaaggttc accagtttag
      961 attgagaaat ttcaccattt ttgtattttt ccatcagtat aaataatact tctgtcagat
     1021 tctttgttat cgcatgatat ataactctgg aattgatcaa ttcatctggt aaaagtttca
     1081 ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat tacctgcccg ggttgctcca
     1141 gaccataaat tttccaataa aaatcatcaa aaaaatcctt agcggataat tgactcagtt
     1201 cgaaatggct atcacaagta aatacagtac gtttatgagt aacagttcga ttaggatcga
     1261 aaaactgaac aacccatttt tcactttctt gtacgcgctt tattttcaac cctaacgcca
     1321 tttggtggtt taatgtgcat gctgataatg ttctgacaaa gtctccattt tttttcatgt
     1381 tataaaaaaa attagtaata actgttcccc actttttatt ttccacaaaa taaatataag
     1441 ctggtgattt tgaaaaatct tccgtttttt ccaaacatac attttttgca atagcatcag
     1501 gggtgctaag aagctgatag tttaccttcc catttgtttc cataaaattc atttcccaat
     1561 aggaagctag gtgacggcaa actatttgtc ttccatatat atcttttgcc actccattta
     1621 tatttacctc cacatttctc ttttctgaaa aataaggtgc tgttttattc ttaaactgaa
     1681 ttagttcact ggagtttgag accctcataa tagaacttgt tattgttcta ttatctctgt
     1741 taacagacat atctgttgat aacgaatttt ttgttgaaaa tatactagat gaaaatgttt
     1801 tatttaacgg catagaagac aaccatagag atgttataat actattagat tgcagttact
     1861 aatagtaaaa gcctccttac aaggaggtag cttattcaga atgctcctat aagatagcga
     1921 gctcgtcaac gatacagatg atctgattat attgtgcaaa aggagaacag gaaaataggc
     1981 actggaaaag atgaaggcct gatgggagca ttaactaatg caaaatccga caggttaata
     2041 tcccgaaaag caatcaaacg actcaaacag gagatgcacg ctgaaatggt ggacaactgt
     2101 tcggatttca gatgagtagc tcagcagttt atgagccgaa tgctctgtca cgggcaggaa
     2161 tcaggctgca tacgcagtgc caaatctaat cgtagttatt ctaaccagtt ttcaagctac
     2221 aaggtggtgt agctggcatc ttctctactc tatcatcaaa cgataaaatg gttcctctcg
     2281 taacatttgc tcttgccttt cacttccatc tcgatcgctt cgtagttcat tacgatagct
     2341 gacatatttc cagtcttcag gtctaacatc tgacgccgga tactccccgg gagcgatttt
     2401 gcttcttaat agattagact cgagacacat tataaattgt cgtattttat gaagtaataa
     2461 agcactatat tgcgagcatt cctgatctga ttttacatat agcgtaaact gagctcctat
     2521 ccccacacga gattgctgag aaactcgatt catatcagtt atcttccatt tatctatagg
     2581 actgtcctct gaaaataata aaccagacaa aatttggaat gccagaggaa cttgctccct
     2641 agctatacta atatgaaact tgtccccaac aaaatccccc ttactctgag gattttcacg
     2701 acatgcatga ataaacacat ctccactatg gttcacaatc tggaatcctt ggtacatgac
     2761 agaaaaagat tgacatatat cagagtaagt tggcacgttc gatgctactt tgaaatagta
     2821 cgctgggtat tgccgtattt ggttgtacaa tatattgaag ttatttttca atctttcatt
     2881 cgcagaaagc atctgcggta tttcagacct cacaacattc aaagaatcta aatttagttt
     2941 tagacagggc ttttttatgg gcatagaaaa cgtcctctat aaaatagata tattatctta
     3001 tattgattgt ttatcatctt ttataaaacg atacattata tgtaatgtat gtaagcgtaa
     3061 gggtcctctc cgaacatggt gggaaggctt agaacgc
//
LOCUS       DAZXUL010000257         3069 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZXUL010000257 DAZXUL010000000
VERSION     DAZXUL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.258"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..498
                     /locus_tag="ACH3L1_004392"
     CDS             <1..498
                     /locus_tag="ACH3L1_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4078845.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="ACH3L1_004393"
     CDS             578..1273
                     /locus_tag="ACH3L1_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFT4078846.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="ACH3L1_004394"
     CDS             1302..>3069
                     /locus_tag="ACH3L1_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFT4078847.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAZXUL010000258         3008 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZXUL010000258 DAZXUL010000000
VERSION     DAZXUL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.259"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            110..628
                     /gene="mddA"
                     /locus_tag="ACH3L1_004395"
     CDS             110..628
                     /gene="mddA"
                     /locus_tag="ACH3L1_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFT4078848.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            625..1074
                     /locus_tag="ACH3L1_004396"
     CDS             625..1074
                     /locus_tag="ACH3L1_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFT4078849.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1075..1308)
                     /gene="ydcY"
                     /locus_tag="ACH3L1_004397"
     CDS             complement(1075..1308)
                     /gene="ydcY"
                     /locus_tag="ACH3L1_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFT4078850.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1394..1567)
                     /gene="ortT"
                     /locus_tag="ACH3L1_004398"
     CDS             complement(1394..1567)
                     /gene="ortT"
                     /locus_tag="ACH3L1_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFT4078851.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1762..1857
                     /gene="yncL"
                     /locus_tag="ACH3L1_004399"
     CDS             1762..1857
                     /gene="yncL"
                     /locus_tag="ACH3L1_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFT4078852.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2259..>3008)
                     /locus_tag="ACH3L1_004400"
     CDS             complement(2259..>3008)
                     /locus_tag="ACH3L1_004400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFT4078853.1"
                     /translation="TKKHFEFCMDNKIFLNHKGKKSYKERNLFLFSKGDKITIEDNVI
                     AEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNVKIMGGNHPLNRFTTHMM
                     TYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDVVLKGGIAIGDGAVIAA
                     NSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNYNYSDLPDNNKCDDINY
                     FVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
BASE COUNT          798 a          659 c          647 g          904 t
ORIGIN      
        1 ataagtgata ctggttgtct ggagattcag ggggccagtc tatccgttgc ttcgggctgt
       61 tttcgatagt attgataaca tcatcatcat tattttattg aggccagaca tgtccatccg
      121 ttttgcccgc aaagccgact gtgctgccat tgcggaaatt tataaccacg ccgtgttgta
      181 tacggcggct atctggaatg accaaacggt ggatgcagat aaccgcattg cctggtttga
      241 agcgcggacc atagcaggtt atccggtgct ggtaagtgaa gaagatggcg tagttacggg
      301 atatgcctcg tttggcgact ggcgtagttt cgatggtttt cgccataccg tggaacattc
      361 ggtttatgtc catcccgatc atcagggcaa aggtctgggg cgtaaattgt taagtcgatt
      421 gattgatgaa gcgcgggatt gcgggaagca tgtcatggtc gccgggatcg aatcgcaaaa
      481 tcaggcttcg ctgcatctgc accagtcgct gggatttgtc gtcaccgcac aaatgccgca
      541 ggtaggcact aaatttggtc attggctgga tctgacattt atgcagttgc aactcgacga
      601 gcgcactgaa ccggacgcga ttggatgaat cagtcgctca cccttgcttt tttgattgcc
      661 gccggaattg gtctggtagt ccagaacacg ctgatggcgc gcatcaccca gacctcctct
      721 accattctca tcgccatgtt gctgaactca ctggtgggga ttgtgctctt tgtttctatt
      781 ttatggttta aacaaggcat ggcggggttt ggcgaactgg tgtccagcgt gcgctggtgg
      841 acacttatac ctggcttgct gggttcgttt tttgtcttcg ccagtatcag cggatatcag
      901 aatgtgggag cggcgacaac tattgcggta ctggtcgcca gccagctgat tggcggtctg
      961 gtgctggata tcttccgcag tcatggcgta ccactacggg cgttgttcgg cccaatatgt
     1021 ggcgcaattt tacttgtggt tggcgcctgg ttggtggcca gacgctcatt ttaatcagag
     1081 gatggtgccg cctttggtta actgttcgtg gcgcgcttcc atatcttctt tatgcttgcg
     1141 accgtgatga gcaatagcgg tgcgcaaacg ctgctgctgg gtgtagcgat cttcccgact
     1201 taactccgcg tcatcgctca gggcgattaa taattcgttc atatgggcaa tgacgctctc
     1261 ttcaatcgcg gcatccacgc gcgcgatgac ttcatccaga tgcgacatac tttctcctta
     1321 gccacttgtg aaatttatcg attaatccaa atgatataag tctgcgtggt gccgtcaagc
     1381 cagtgagtaa gatttaaaac agtgaaaaca acagcgccac cgggaaactc atcggccatg
     1441 tggcccccac cagaaaggcg cttaagaaac ggatgcgttt cttatcgtga gaaagaaacc
     1501 aggtgataag aaatgcgatt gctgccataa ccgcataaaa aacaagcata tgttgataga
     1561 gaaacatgta cgggcgtcca gccatatgaa taaaacgcgc gcaaatatgc gccatttttt
     1621 gacgtgcatc aagttttttc accagtttga gaaaaaatca tcaaacagca acttgccctt
     1681 atccatcttc agaatgagta ttattgcgga ttttcttaac ccgtactata cacatagggg
     1741 caatgataaa aggtggcaaa aatgaatgtt tccagtagaa ctgtagtact gataaatgtc
     1801 tttgctgctg ttggtttgtt tactcttatc tctatgagat ttggctggtt tatttgatgt
     1861 agtgattttg cacgcagcac gtactgtcag agaaatcaac tctctgacag tacgcaagat
     1921 cttctcatac tctccaagtt tgcaacatta tccttaatat ttacttcttt ttgctggcat
     1981 ctacattgaa atgagtagaa aacatccccc tactgtcgtc tatacaaaca gcttactgat
     2041 tcaaacggaa tcttgttttc acatttttct atcacgaata ctttatacgc gcgggagtct
     2101 ataaatgctt tatactttca aggaataacg taaatataaa aagatggcgt aaagtatatg
     2161 catggcagga tgtttgcaac ctccttgatg acgctgcccc attgaaattg caaaccccgc
     2221 cctggttatc ttgcccattt atcatgcaaa acagataatt ataggcctct gaatacctca
     2281 gaaagattga attttttata gtccctctct tgaatatttc catttgatat caatcgattc
     2341 atctcttcaa caaaataatt tatatcatca cacttattat tatccggaag atctgagtag
     2401 ttataattcc accattttat tctcaacagt tcatcaataa catttgaatc aaacctaaat
     2461 ctgattattt ttgcaggaac tcctgcaact atggcatatg ggggcacatc tttggtaaca
     2521 acggaattgg cagcaataac agcaccatca cctattgcga tgcctccttt caatacaaca
     2581 tcatttccta tccagacatc atttcctata atcggatttt caggtttggt aatgaatggt
     2641 ttcaatgtcc agctacgttc aaattcactc atagcaaact tatcaaattc accgttataa
     2701 gtcatcatgt gagtagtaaa acgattcaaa ggatgatttc cacccattat ttttacattt
     2761 gatgcaatac tacaaaatcg acctatacgt atattccctg aaaaatggca ggtcggaaat
     2821 gaaaaagcgc cgacactgga gaaatttttc acaggcattg ttgaatattc ttcagcaata
     2881 acattatctt caattgttat cttgtcacct tttgagaaaa gaaataaatt cctttcttta
     2941 tacgacttct tccccttatg atttaaaaag attttattat ccatacaaaa ctcaaagtgt
     3001 tttttcgt
//
LOCUS       DAZXUL010000259         3006 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZXUL010000259 DAZXUL010000000
VERSION     DAZXUL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.260"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACH3L1_004401"
     CDS             complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACH3L1_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFT4078854.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            348..641
                     /locus_tag="ACH3L1_004402"
     CDS             348..641
                     /locus_tag="ACH3L1_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFT4078855.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="ACH3L1_004403"
     CDS             713..1372
                     /locus_tag="ACH3L1_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFT4078856.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="ACH3L1_004404"
     CDS             1464..1910
                     /locus_tag="ACH3L1_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFT4078857.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3006)
                     /gene="hlyE"
                     /locus_tag="ACH3L1_004405"
                     /pseudo
     CDS             complement(2117..3006)
                     /gene="hlyE"
                     /locus_tag="ACH3L1_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT          784 a          649 c          720 g          853 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacg
//
LOCUS       DAZXUL010000260         2977 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZXUL010000260 DAZXUL010000000
VERSION     DAZXUL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.261"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            122..805
                     /locus_tag="ACH3L1_004406"
     CDS             122..805
                     /locus_tag="ACH3L1_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFT4078858.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            806..1027
                     /locus_tag="ACH3L1_004407"
     CDS             806..1027
                     /locus_tag="ACH3L1_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078859.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            957..1475
                     /locus_tag="ACH3L1_004408"
     CDS             957..1475
                     /locus_tag="ACH3L1_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFT4078860.1"
                     /translation="MAPIACSWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAKY
                     PGDTPLMLVIWSPEEIQALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSATAM
                     EIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPECITRRQAVVNQA
                     RILLKNNPHEND"
     gene            1520..2290
                     /locus_tag="ACH3L1_004409"
     CDS             1520..2290
                     /locus_tag="ACH3L1_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078861.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDVSRLATLLISRHDGVLPPVLASAMQKLTGTVAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRDAVRCVSYRVAEDTRRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSVADALAKIRG"
     gene            2703..>2977
                     /locus_tag="ACH3L1_004410"
     CDS             2703..>2977
                     /locus_tag="ACH3L1_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFT4078862.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIIL"
BASE COUNT          611 a          844 c          885 g          637 t
ORIGIN      
        1 gctgcggtat tccacggtcg gcctgacccg ttaccggacg cggtgaacag cccacaggca
       61 gcggggaacg ggcaccacag gggtgccctc ccggtgccct ctgtaagaga aggagttttg
      121 tatgtcccgt tttgtcctcg gtaactgcat cgatgttatg gcccgtattc ctgataacgc
      181 cattgatttc atactgactg acccgccgta cctcgtcggt ttccgtgacc gttccgggcg
      241 caccattgcc ggtgatgtta atgatgactg gctgcaacct gccagcaatg agatgtaccg
      301 cgtactcaaa aaagacgcgc tgatggtcag cttctacggc tggaaccgtg tcgatcgctt
      361 tatggccgcc tggaaaaatg cgggattcag cgtggttggg cacctcgtgt tcaccaaaaa
      421 ctacacatcc aaatctgcgt atgtcgcgta ccgccacgaa tgcgcttaca tcctggcaaa
      481 gggcagtccg gcccggcccc gtaaccctct gccggatgtg ctgggctgga aatattcagg
      541 caaccgccat cacccgacgg aaaagcccgt taccagcctg caaccactga ttgagagctt
      601 cacacatccg aacgcgattg tgctggaccc gtttgcaggc agcggctcaa cctgcgtcgc
      661 tgccctccag tccggacgcc ggtatatcgg tatcgagctg cttgaacagt atcaccgtgc
      721 cggacagcaa cgactggccg ccgtgcaacg ggccatgcag cagggggccg cgaatgataa
      781 ctggtttgaa ccggaggctg cgtaaatgaa ctatgccgga cacgaaaaac ttcgcgccga
      841 cgtggcggag gtggccaaca ccatgtgtga ccttcgggcc aggctgaacg atatggagca
      901 tcgctgtcgc tttgattccg atgtgcttgt tgaacgcctg acacgccaga cgctgtatcg
      961 cgccaatcgc ctgttcatgg aggcgtatac cgaaattctg gaactggatt cctgcttcaa
     1021 agattgagaa ggagacgaga atgtacggaa catgcgaaac gctatgccgg gagctggcag
     1081 caaagtatcc gggagacacg ccactgatgc tggttatctg gtccccggaa gaaattcagg
     1141 ccctcgctga cggaatggat atttccctgt ccgatcatga aatcaggact gtcctggcgc
     1201 gcctggagga catcccggaa gaccagcgga ctgaatccgg tatttcttcc gccactgcga
     1261 tggagattat cagcaacgtg agcgaaaacc gccaggtgac cgtcccggct gaactgctgg
     1321 cgtccctgat tcagaccgcc gaacaggcac tgtggaaacg tgaatgggcc gcccgtgatt
     1381 acggcctcgc cgtcccggaa tgcattaccc gccgtcaggc ggtggttaat caggcccgca
     1441 tcctgctgaa aaacaaccca cacgaaaacg actgatgtta tcgccgcctt cgggcggcga
     1501 ttcagggaga ccattaatca tgaacaaaac gctgaatgca ctggtttgtc gccacgcccg
     1561 taacctgctg ctggcgcagg gctggccgga agagacggat attgaacaac tgaatccgca
     1621 ctatccgggc tggatcagca tttatgtgcg actggatgta tcccggctgg cgacgttact
     1681 tatcagccgc catgatggcg tgctgccgcc ggtcctggcg tctgccatgc aaaaactgac
     1741 cggaaccgtg gcggaacttg tactgtccgg cagccagtgg caggcgctgc cggtacttcc
     1801 ggcagacgga acgcaggtgt ctttcccgta tgccggtgaa tggctgacgg aggaagaaat
     1861 cagggcagtt cttgatgcgg tgcgcgatgc agtacgctgt gtcagttacc gggtggcaga
     1921 agatacgcgg cgtatccggg cggcgctgac cacaaccggt cagacgttac tgacccgcca
     1981 gacgcgccgc tttcgcctgg tcgtgaagga aagcgattac ccctgctggc ttgatgaaga
     2041 tgacgaaaat ctgcccgtgg tgctcgatgc cattctgaac cgtggcgcac gtttttcctc
     2101 tgtggaaatg tacctggtca gtgagtgtgt ggagcatatc ctgtccagtg ggctggtctg
     2161 tgatgtgctg cgtataccgg atgaaccgtc ccgtcgctgg tttgacaggg atattctgcg
     2221 ggaggttgtt ctggaggcac gggatgaaat ccgtagcgtg gcggatgccc tggcaaaaat
     2281 caggggctga tgatggcggg gaaaatcccc gcctttttcc tgccggtccc cggacagagc
     2341 acaaaggaag tcgccgtcgg ctccgcttta cccggccatg caaggcatgg ccttgtggtt
     2401 tttcagttat gtggcctcag cgtcgtgtgc gggctgtgcc gtgcctccat cgtagccggg
     2461 cttctgcggg cgggttgcaa ggtaaggtga accgctgcgg tcacccggtc cctccttccc
     2521 gtctgccgtg attttccggt cgttcacccg ctcagatttc gcggtctcac cctccaactc
     2581 ccgtcagccg gcgcgtcagg ctgcggcttc ccttgcgccc ttgcaaccct gcaggcccgg
     2641 caaagatgga ggcacggcac cgcccggtgt cgcggaatat gtgaacaacg gaggaaaaca
     2701 tcatgcaata tgcgaaacct gtcactctga acgttgaaga gtgcgaccgt ctgtcttttc
     2761 tgccttacct gtttggcctg gattttctgt atgccgaagc gtctgtatac gcgctggcga
     2821 aaaaaatgat gccggaatat gaaggcggat tctggcactt catccgcctg ccggacggtg
     2881 gcggttacat gatgccggat ggcgaccgtt tccacatggt gaacggtgca aactggtttg
     2941 accgtaccgt gagtgctgat gccgcaggca tcatcct
//
LOCUS       DAZXUL010000261         2956 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZXUL010000261 DAZXUL010000000
VERSION     DAZXUL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.262"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(173..1597)
                     /gene="patD"
                     /locus_tag="ACH3L1_004411"
     CDS             complement(173..1597)
                     /gene="patD"
                     /locus_tag="ACH3L1_004411"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFT4078863.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIHRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1619..2413)
                     /locus_tag="ACH3L1_004412"
     CDS             complement(1619..2413)
                     /locus_tag="ACH3L1_004412"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFT4078864.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2403..2750)
                     /locus_tag="ACH3L1_004413"
                     /pseudo
     CDS             complement(2403..2750)
                     /locus_tag="ACH3L1_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT          747 a          865 c          701 g          643 t
ORIGIN      
        1 cgatactcta aagccttatg ctaatcaact gtgacaggat gaaaaatcgc catcaaacat
       61 ttagcctaat ttttacgcca gttattaatg taatcattct aatacttaac cgccattaaa
      121 ctcagaacct aaaacgatag tggccgtcct ataacagcca ctaacatcta acttaatgtt
      181 taaccatgac gtggcggacg acggtgtaat cctccagccc ataaagtgac atatccttgc
      241 cgtaaccaga aagtttctgc ccaccgtgcg gcatttcact taccagcatg aaatgggtat
      301 tgacccaggt acaaccatat tgcagccgtg cgctgacgcg atgcgccctg cccacatctt
      361 tcgtccatac cgaagatgca agtccgtact ggctgtcatt cgcccagttc accacctgtt
      421 cttcgttgtc gaagggcgta acactcacta ctggaccaaa tacctctttt tgcacgatgg
      481 catcgtcctg taatgcgcca gccagcagcg tcggcgcata gtaatagcca ttacccttgc
      541 gcttttcacc gccagtgatc actttgatgt gccctgtcgc tttcgcctct tctactgcct
      601 tgctgacgcg ttcgagatgc gccagcgagc ttaaaggtcc aagctccgta gactcgtcat
      661 ccggcgcacc agattttaac gttgccaccg cagcacccag tttttccacc agcgtatcgt
      721 aaatgccttt ttgcgcgtag atccgacaag ccgcagtaca atcctgtcca gcattgtaat
      781 agccaaatgt acgtacacct tcgaccactg cttcaatatc cgcatcatca aaaacaatca
      841 ctggcgcttt gccaccaagt tccatatgag tacgcttaat ggacgacgcg gtatggctga
      901 tgatgtgctc gccggtggcg atagagcccg tcagcgacac catccgcact ttgggatgac
      961 cggtcagcgg atcacccacc gttttgcctc tgccaaacag tatgttaatc acgcctgccg
     1021 ggaagatatc tttcgccagc tctgccaact tcaacgcggt cagcggggta atttctgatg
     1081 gtttaagcac tacgcagttc cctgccgcca gcgccggagc aagtttccac gcggccatca
     1141 tcagcggata attccacggt gcgatagaag ccacgacccc caacggatcg cgatggatca
     1201 tcgaagtatg accttcaaga tattcacctg ccgccagacc attcagacag cgcgccgcac
     1261 ccgcgaaaaa gcgaaaaaca tcgacaatcg ccgggatttc atcattgaac gcactatgca
     1321 gcggtttgcc acaattacgg gattccagtt cggcaaaaac ctgaccattt tcttcgataa
     1381 catccgccag tttcagcagg cattccgcac gcgctttcgg tgtggtttgc ccccattcag
     1441 caaatgctgc atctgccgca cgcacagcag catcgacctg ctctgcggat acctcggcaa
     1501 tctccagtaa aacgtccccc gttgccggat tatagacagg ctgtttttcc ccttcgccgc
     1561 taacccgttc tccgttaatc agtaacttat gttgcatagc ttattcctgt atcagtggtt
     1621 attgaccatt gtcgccttcg cgggttagcc accaggcccc caggatcggc aaggttgtta
     1681 ccaacataac cagcagtgcc accacgttgg ttaccggtac atcacgcggt cgcccaagct
     1741 gattgagcaa ccacaacggt aacgttcgtt catgacctgc cgtaaaggtc gtaacgatga
     1801 tttcatcgaa cgacaaggca aacgccagca ttcctcctgc cagtaacgcc gaactgagat
     1861 tcggcaacac tacgtagcgg aaggtttgcc agccattggc cccaagatcc attgacgcct
     1921 caaccagact ccacgaggtg cggcgaaaac gggcgatgac attgttaaac accaccacta
     1981 cgcaaaaagt cgcatgaccg accacgatgg tgaaaaatcc cggctccaga ttgatggttt
     2041 taaaggcggt taatagcgcc agaccagtga caatgcccgg cagcgcaatg ggcagcagca
     2101 ataacagcga aatggcgttt ttgccgaaaa agtctcgtct ccacagcgcg gcagctgcca
     2161 gcgtccctaa caccagcgca attaatgtcg ccagcgccgc cactttaagt gacagcgtca
     2221 cggcatcaag aatatcacta cgctgtgctg ccacgctaaa ccagcgcagc gtcaggccct
     2281 gcggtggaaa actaaacgcc gcatcttcag tgttaaacgc ataggcggcg attatcagga
     2341 tgggaaaatg taggaaaaca acgccgcccc aggccgccag tttgaggaaa aacggtgcgc
     2401 gttcagagtg catcgaacgc tcccagacgt ttcacgaacg ccaggtacag tgcgatgaga
     2461 ataatcggca ccagggtgaa tgccgccgcc atcggcatat tgccaatcgc cccctgctgg
     2521 gaataaacca tattgccgat aaaatatcct ggaggaccaa ccagctgcgg gacgataaaa
     2581 tcgcccagtg tgagtgagaa ggtaaagata gagccagcgg caatacccgg gattgccagc
     2641 ggcagcacca catagcgaaa ggtttgtcgt ggacgtgcgc cgagatcagc cgacgcctgc
     2701 aacaatgacg gcggcaaacg ctcaagcgcc gcctgaacgg gcaggatcat cgagtaggca
     2761 gcctgacggc tgcggcttgt catggtctgc aattaccgtt ataaaaacag gcaatgtcat
     2821 tgtctttcag gtggttatat ggcccgttca gctaaacccc gtaaacgtaa acccgcctct
     2881 caaagttcct gagtcgtgac ccgttcgaat gcgtgctgtg tggcggccgg atggtatacc
     2941 gccgggccat tgcggg
//
LOCUS       DAZXUL010000262         2951 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZXUL010000262 DAZXUL010000000
VERSION     DAZXUL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.263"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="ACH3L1_004414"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="ACH3L1_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFT4078865.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="ACH3L1_004415"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="ACH3L1_004415"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFT4078866.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="ACH3L1_004416"
     CDS             2603..>2951
                     /locus_tag="ACH3L1_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFT4078867.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAZXUL010000263         2932 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZXUL010000263 DAZXUL010000000
VERSION     DAZXUL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.264"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACH3L1_004417"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACH3L1_004417"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFT4078868.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACH3L1_004418"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACH3L1_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFT4078869.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACH3L1_004419"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACH3L1_004419"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFT4078870.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2932
                     /locus_tag="ACH3L1_004420"
     CDS             2756..>2932
                     /locus_tag="ACH3L1_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078871.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          660 a          800 c          805 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacacctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DAZXUL010000264         2898 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZXUL010000264 DAZXUL010000000
VERSION     DAZXUL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.265"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..312)
                     /locus_tag="ACH3L1_004421"
     CDS             complement(<1..312)
                     /locus_tag="ACH3L1_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFT4078872.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DI"
     gene            complement(359..1078)
                     /gene="btsR"
                     /locus_tag="ACH3L1_004422"
                     /gene_synonym="yehT"
     CDS             complement(359..1078)
                     /gene="btsR"
                     /locus_tag="ACH3L1_004422"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFT4078873.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="ACH3L1_004423"
     CDS             complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="ACH3L1_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFT4078874.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          750 a          774 c          658 g          716 t
ORIGIN      
        1 aatgtcatcg gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt
       61 aatgcgacgt tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat
      121 caggccattg aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc
      181 ttctttacgt agccagacgg cgatctgttc cgcggtggct tcgacattat ccagcgccag
      241 aatacgcacc aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc
      301 gttacttatc atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt
      361 acaggccaat cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc
      421 cattacgcaa aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg
      481 cgaggttaac cagatactga cgatggcagc gcagtagtgg tgtacgactt tccaaggtac
      541 gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca
      601 tccgactgct gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg
      661 tacaagggat aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc
      721 tgcgctcctg acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca
      781 gcagataatc aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga
      841 gaaaaacaat atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga
      901 tgcgcggcat ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga
      961 tcccttccac ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa
     1021 atatacgcag gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc
     1081 ctcgtccctc catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac
     1141 gcttattcca tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc
     1201 cagcccactg gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat
     1261 caaatgttgc ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga
     1321 tgtcccatgt ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg
     1381 ctgggataat tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt
     1441 ttcaatttgc agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga
     1501 aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg
     1561 ttcgctgtcg cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg
     1621 attcacctgg gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc
     1681 atattgccct gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg
     1741 gtttattgaa ctgaataacc ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg
     1801 ctgattttcg ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa
     1861 agagcaacga taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc
     1921 ttttaaggtg taagtcgaag aaatcggttt gccgggtaaa tggtggtcgt caccaattcc
     1981 ggtaaaggcc agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg
     2041 atacagtacc tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc
     2101 ttccgtcgag gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa
     2161 catcgcgcgt ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac
     2221 catcattggc gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat
     2281 cgcaaggatg atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat
     2341 ggggttaaag actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc
     2401 gagcaatcct tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga
     2461 atatcgatgt aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa
     2521 gccgcccatt accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc
     2581 aaaccaggtg cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg
     2641 cagacgaacc gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc
     2701 aatgactaaa aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat
     2761 acccgcaaac cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct
     2821 ttgaactctt gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa
     2881 aataaaatca ccctatag
//
LOCUS       DAZXUL010000265         2887 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZXUL010000265 DAZXUL010000000
VERSION     DAZXUL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.266"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="ACH3L1_004424"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="ACH3L1_004424"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFT4078875.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="ACH3L1_004425"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="ACH3L1_004425"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFT4078876.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1132..1890
                     /gene="tam"
                     /locus_tag="ACH3L1_004426"
     CDS             1132..1890
                     /gene="tam"
                     /locus_tag="ACH3L1_004426"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFT4078877.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1894..2808)
                     /locus_tag="ACH3L1_004427"
     CDS             complement(1894..2808)
                     /locus_tag="ACH3L1_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFT4078878.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          759 a          667 c          771 g          690 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aatgtctgac
     1141 tggaacccct ctttatatct gcacttttcc gctgaacgat cgcgtccggc ggtggagctg
     1201 cttgccagag tgccgctgga gaatgtcgaa tatgttgccg atcttggctg tggcccaggt
     1261 aacagcaccg ccctgctgca tcaacgttgg cctgcggcca ggataacagg catcgactcg
     1321 tctccggcga tgattgctga agcgcgcagt gctttgccag actgccagtt cgtggaagca
     1381 gacatccgca actggcaacc ggaacaggca ctcgatctga tttttgctaa tgcctcactg
     1441 caatggctgc ccgaccacta cgaattgttt cctcacttgg tttctttact taatccgcag
     1501 ggcgtgctgg cagtacagat gccagataac tggctggagc cgacccatgt gctcatgcgc
     1561 gaagttgctt gggaacaaaa ctacccggat cgcgggcgtg agccgttggc tggcgttcat
     1621 gcttactacg atattttgag cgaagccgga tgtgaggtcg atatctggcg aaccacttac
     1681 tatcaccaga tgccgtcgca ccaggcgatt atcgattggg tgaccgccac tggattacgt
     1741 ccgtggttac aggatctgac cgagagcgaa cagcagcttt ttcttacgcg ctaccatcag
     1801 atgctggaag agcagtatcc actgcaagag aacggacaga tactgctggc atttccgcgt
     1861 ctgtttattg ttgcccgccg tacggagtaa ttatcacgtc agctggtaat gacgatcggg
     1921 aagaattttt gctggaattt ctgcttcatc gttcatctgt aacaggtcaa tttcaatagc
     1981 gttgcagatg gcatccagcg gtaaatcatt gttttcagta ccgaacggat cttccagttc
     2041 ttccgccaga caatccagcg aaataaaagt gtaggaaatc agcacagaga taaaaggcgt
     2101 catgtaatgc aggtccacga ccagcgcgaa cggcagcatg atacaaaaca gataaacggt
     2161 acgatgcaaa atcagggtgt aggcaaaggg aattggcgtg taggcaatgc gctcacagcc
     2221 tgccagaacc gctgaaatat cattaagccg atcgttgagg ctaataaaca ggatatctga
     2281 aagctgtcca ttgcggcgct gaaccgccaa ccattctccc attattaaca agatacggtt
     2341 agctggagag ttcgaagcca gtacacgctg aagatctcca gtcttgagat aatgagccag
     2401 cacttccgcc tgtggctgtt tgcgtaatgt catgcgtaaa cagtgggcga aggcgatttg
     2461 cagcctggca aactccctta cacttgccga atccggcaat gtcgttttta cctctcgcag
     2521 taacgaccgt gaggcaatca tcaactgccc ccaaagtttt cgcgcttcaa cgtaacgggc
     2581 gtacccggca ttattacgaa aaccaagaaa aatggcgatg gcgacaccga gaatgctgaa
     2641 cggtgcgagg gtgaatttga tgcccagatg cgtgtaccag ggcagcatga atataacagc
     2701 aatagaaaag agaaaattga gtagtaagcg cgaggatatc ttggataata ctgagccgtg
     2761 ccagacaaaa atacggcgca gccagtgttg ttgtggacga acaatcatgg ttatcttcag
     2821 gcgtggaaaa gtcgccctat taaacgtgat tacgatcaca ttctcaagac gctacttaca
     2881 aattacc
//
LOCUS       DAZXUL010000266         2855 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZXUL010000266 DAZXUL010000000
VERSION     DAZXUL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.267"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            43..1440
                     /gene="ccp"
                     /locus_tag="ACH3L1_004428"
     CDS             43..1440
                     /gene="ccp"
                     /locus_tag="ACH3L1_004428"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFT4078879.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1651..>2855
                     /locus_tag="ACH3L1_004429"
     CDS             1651..>2855
                     /locus_tag="ACH3L1_004429"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFT4078880.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLR"
BASE COUNT          730 a          652 c          753 g          720 t
ORIGIN      
        1 attttctggg tcacaataac tcaccaccgt gagggaagaa taatgaaaat ggtctcacgt
       61 attaccgcga tcggcctggc tggcgtcgcg atttgctatt tagggttatc tggttatgtg
      121 tggtaccacg ataataaacg cagtaaacag gccgatgttc aggcatctgc tgtcagtgaa
      181 aataataagg ttttaggctt tctccgcgaa aaaggatgcg actattgcca cacgccttcg
      241 gcagaattac ccgcctatta ttatattcct ggcgcgaaac agttgatgga ttacgacatt
      301 aagcttggat ataaatcttt taacctcgag gccgtgcgtg cggcactgct ggctgataaa
      361 cccgtttcgc aaagcgatct gaataagatt gaatgggtga tgcagtatga aactatgcca
      421 ccaacgcgtt ataccgcgct acactgggcg ggtaaggtga gtgatgaaga gcgggcggaa
      481 atactggcct ggattgcaaa acagcgcgcg gaatattacg ccagcaatga tactgctccg
      541 gagcatcgca atgaaccggt gcagcccatc ccgcaaaaac tgcctaccga tgcgcaaaaa
      601 gtggcgttgg gctttgcgct gtatcacgat ccccgtttat cggctgatag caccatttca
      661 tgcgctcatt gccatacgtt gaatgcgggg ggcgtcgatg gcagaaaaac atcgattggt
      721 gttggtggcg cagttgggcc gattaatgcg ccgacggtat ttaactcagt atttaacgtt
      781 gagcagttct gggatggtcg tgcggcaaca ttgcaggatc aggcaggtgg accgccgttg
      841 aacccgattg aaatggcgtc gaaatcctgg gacgaaatta ttgctaagct ggaaaaagat
      901 ccgcagctta aagcgcagtt cctcgaagtt tatccgcaag gtttcagtgg cgaaaatatt
      961 actgatgcca ttgctgaatt tgagaaaaca ttaattacgc cggattcccc atttgataaa
     1021 tggttgcgtg gagatgaaaa tgcgctgacg gcgcaacaga aaaaaggcta tcaattattt
     1081 aaagataata aatgtgcaac ttgtcatggt ggtattattc tcggcggacg ttcctttgaa
     1141 ccgttggggc tgaaaaaaga ctttaacttt ggggaaatta cggcggcgga tattggtcgt
     1201 atgaatgtga ctaaagaaga gcgtgataaa ttgcgtcaga aagtacccgg tttacgtaac
     1261 gttgctttaa cggcaccgta cttccatcgc ggtgacgtgc cgacgctgga cggggcggtg
     1321 aaactgatgc tgcgctatca ggtaggcaaa gagctgccgc aggaggatgt ggatgatatc
     1381 gtagctttcc tgcacagtct gaacggggtg tacacgccgt atatgcagga taaacaataa
     1441 ttaatttgat cgcccgaaca ggaatgtttg ggcgattttt attacgataa taaagtctgt
     1501 ttttaatatt atcatgttaa atgtttatat tataaaaagt cgtttttctg cttaggattt
     1561 tgttatttaa attaagcctg taatgccttg cttccattgc ggataaatcc tactttttta
     1621 ttgccttcaa ataaatttaa ggagttcgaa atggaccaga agctgttaac ggatttccgc
     1681 tcagaactac tcgattcacg ttttggcgca aaggccattt ctactatcgc ggagtcaaaa
     1741 cgatttccgc tgcacgaaat gcgcgatgat gtcgcattcc agattatcaa tgatgaatta
     1801 tatcttgatg gcaacgctcg tcagaaccta gccactttct gccagacctg ggacgacgaa
     1861 aacgtccata aattgatgga tttgtcgatc aataaaaact ggatcgacaa agaagaatat
     1921 ccgcaatccg cagccatcga cctgcgttgc gtaaatatgg ttgccgatct gtggcatgcg
     1981 cctgcgccga aaaatggtca ggccgttggc accaacacca ttggttcttc cgaggcctgt
     2041 atgctcggcg ggatggcgat gaaatggcgt tggcgcaagc gtatggaagc tgcaggcaaa
     2101 ccaacggata aaccaaacct agtgtgcggt ccggtacaaa tctgctggca taaattcgcc
     2161 cgctactggg atgtggagct gcgtgagatc cctatgcgcc ccggtcagtt gtttatggac
     2221 ccgaaacgca tgattgaagc ctgtgacgaa aacaccatcg gcgtggtgcc gactttcggc
     2281 gtgacctaca ccggtaacta tgagttccca caaccgctgc acgatgcgct ggataaattc
     2341 caggccgaca ccggtatcga catcgacatg cacatcgacg ctgccagcgg tggcttcctg
     2401 gcaccgttcg tcgccccgga tatcgtctgg gacttccgcc tgccgcgtgt gaaatcgatc
     2461 agtgcttcag gccataaatt cggtctggct ccgctgggct gcggctgggt tatctggcgt
     2521 gacgaagaag cgctgccgca ggaactggtg ttcaacgttg actacctggg tggtcaaatt
     2581 ggtacttttg ccatcaactt ctcccgcccg gcgggtcagg taattgcaca gtactatgaa
     2641 ttcctgcgcc tcggtcgtga aggctatacc aaagtacaga acgcctctta ccaggttgcc
     2701 gcttatctgg cggatgaaat cgccaaacag gggccgtatg agttcatctg tacgggtcgc
     2761 ccggacgaag gcatcccggc ggtttgcttc aaactgaaag atggtgaaga tccgggatac
     2821 accctgtacg acctctctga acgtctgcgt ctgcg
//
LOCUS       DAZXUL010000267         2849 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZXUL010000267 DAZXUL010000000
VERSION     DAZXUL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.268"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..197
                     /locus_tag="ACH3L1_004430"
     CDS             <1..197
                     /locus_tag="ACH3L1_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFT4078881.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="ACH3L1_004431"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="ACH3L1_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFT4078882.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="ACH3L1_004432"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="ACH3L1_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFT4078883.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="ACH3L1_004433"
     CDS             1821..2576
                     /locus_tag="ACH3L1_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFT4078884.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          723 a          656 c          691 g          779 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta
     2701 attttagata tacatataac ttgttctatt aataaatcac ctcgtattct gactcggccc
     2761 ctggcgcagc aaagtcaggc atttatactc tttttaacac atagaaaatt catatcaata
     2821 taataaaaac ataaaatatt tgcatatac
//
LOCUS       DAZXUL010000268         2807 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZXUL010000268 DAZXUL010000000
VERSION     DAZXUL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.269"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..81
                     /locus_tag="ACH3L1_004434"
     tRNA            6..81
                     /locus_tag="ACH3L1_004434"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACH3L1_004435"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACH3L1_004435"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACH3L1_004436"
     CDS             complement(299..1057)
                     /locus_tag="ACH3L1_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFT4078885.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACH3L1_004437"
     CDS             complement(1065..2168)
                     /locus_tag="ACH3L1_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFT4078886.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="ACH3L1_004438"
     CDS             complement(2178..>2807)
                     /locus_tag="ACH3L1_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFT4078887.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          739 c          675 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAZXUL010000269         2778 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZXUL010000269 DAZXUL010000000
VERSION     DAZXUL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.270"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..967
                     /locus_tag="ACH3L1_004439"
     CDS             <1..967
                     /locus_tag="ACH3L1_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFT4078888.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1548..2771
                     /locus_tag="ACH3L1_004440"
     CDS             1548..2771
                     /locus_tag="ACH3L1_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078889.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT          925 a          544 c          494 g          815 t
ORIGIN      
        1 agagatgttt gtgtttctcg ccgtcgagct ttcgttgctg tttatagtga ttagtgccgg
       61 tgtcagcctg ataagacaaa aggtgccaga ccataaaatc cagcagatga tgggggccag
      121 aaaagggaga ggctatctcc tggctgctct gttgggagcc gttaccccgt tctgtagttg
      181 ctcgacaata cccatgttac gtggattgtt gtcagcgaaa gccgggtttg gtccgactct
      241 cactttttta tttgtttcac cattacttaa tcccattatc gtcggactaa tgtggatgac
      301 ctttggttgg aaagtcacct tgttgtacgc gattatcgca gccggcgtct ccgttcttgc
      361 cagtattatc ctggattctc tggggtttga acgtcatatc attgccagta aaagctcatc
      421 agcaaattgt tgtgctccag caaaaacttc gccgggaacg acatatacgc ccataaaagt
      481 gagttgttgt agtccagcgg ctaaagccat tgagaacccc gtagttacct gttgcagtac
      541 caaagctgta gtaagtatta atcccataaa actagccacc aaagatgcgt tgcaacagtt
      601 taaagatgta ctgccatatc ttttgttagg agtattaata ggatctttta tttatggctt
      661 tattccctca gcgtggattg ccgctcatgc aggagcagat aatcccttcg ccatcccatt
      721 aagcgccgtt gtcggtattc cgctgtatat ccgggctgag gcggttatcc ctctggcatc
      781 tgttctgatg acaaaaggaa tgggtctggg agcattaatg gcattaatca tcggcagtgc
      841 cggcgcaagc ctgacggaag tgatattact taaatcaatg ttcagaatgc cgatgatagc
      901 tgcattcctg acggttatat taggtatggc tatcttgatg ggctatttga ctcaattgct
      961 attttaaata gatgccagca gtaaactata agatgcatag ttagtgccat atccttctaa
     1021 gtagaaaatc atagacagga taatccttaa aaatcacaaa tgatttaact aatgcattga
     1081 gaatgatgac ggctcggtca acaagtcatc aacagacaat aaccacgtaa tcatgcttct
     1141 ctgacatttt atcggagaag caatgactct ttgtctcagt accctcctcc tttttaaaag
     1201 gcagatttca gtcgctaata caacacattt ttaaatacac tttaacctgg ttcattatta
     1261 cacatctaaa acatcacaaa aatattactt cacacactat cggagaacat aataactttt
     1321 taaaatgagc gttttttgat agttgttaac gttttccttt acgatactat cactgccctt
     1381 tttttataca caaatattac cgggtggaaa tgaaagggaa tttaaaggta attatatcag
     1441 gaagatataa cgcattatat ttattgtgtg tagaagaaaa tccattcctg ttctaattat
     1501 ccaatttaaa aacattagca taaaacagat gatgaataag gaatgttatg tcaattgact
     1561 ttaccccagg tataataaat acatatcacg gcgatattta taactgcaca acaaataccg
     1621 ataatgttaa aacgccagac acaccgaagt ggccttgtga taattgggaa gaacaacaac
     1681 ccattaattc cacattctct ggagaaggat acaaccctga acaatttgat ttagcgcaac
     1741 aacaactcca acaaattaat gcatgccaca cgaacaccac atatacaaat gcagactact
     1801 caaaagttgt ggttcaactt gtaagtctta ttaataacat tgaaacgata agttcgacac
     1861 cacttacgca acagacacaa tcaatactta atcagattca taacattaga tacgaaaagg
     1921 ataaggattc tgtatgtcaa atcattgttg tcgttaatcc agaacccaac aatccaatca
     1981 taactaaaat tctcgtagaa gaaggaatac ccgaaagatt cagtgtccaa accgtttctt
     2041 ccgataataa aaattttgct gggcaacgag ctgacttacc aactgatata agagacattc
     2101 aaagcttgta cttaaaaatg gcgaagctgt atattgagca cagtaaaaat gaaaatagga
     2161 tggaagccct tgctggatgc gactttattg atttcaacat gacagggcaa gatatgtcca
     2221 agctagtcct aaccttatca aaattttatt ttgaagatct acttaatata aatttcactg
     2281 acgcaaattt atttaacact attttttcac acaaggaaaa tcccatcccc aaactacata
     2341 aatatgaaca acatcttgac aaacaaatca atggtttgtt ttctacattg ttaactatta
     2401 atgacaactc tttacgcgcg aaagcggaaa ttgcttcgag aataattgat ttcttagagg
     2461 caaaggtagt gaatctttca tttgatgaca tattaaaata taagcaagag ttcaaaaaaa
     2521 tatgttataa acaacttcaa gagtttacaa caccctctct atacaataaa attcaaaaat
     2581 gggcaacaat gtctaaaaat gaattcatag aatttcatta cgagacactt caacccgaaa
     2641 agatttcata ccccttttat ttaaagagag atctgcctaa tgaaaaagat ataaattatg
     2701 gggtggaaat agaaatacca tctggcaaaa gaattcgctt atcaaatcat tatcagaata
     2761 taataccata atattgat
//
LOCUS       DAZXUL010000270         2749 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZXUL010000270 DAZXUL010000000
VERSION     DAZXUL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.271"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            88..153
                     /locus_tag="ACH3L1_004441"
                     /pseudo
     CDS             88..153
                     /locus_tag="ACH3L1_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..629
                     /locus_tag="ACH3L1_004442"
     CDS             381..629
                     /locus_tag="ACH3L1_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFT4078890.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(745..1014)
                     /locus_tag="ACH3L1_004443"
     CDS             complement(745..1014)
                     /locus_tag="ACH3L1_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFT4078891.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1071..1739
                     /locus_tag="ACH3L1_004444"
     CDS             1071..1739
                     /locus_tag="ACH3L1_004444"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFT4078892.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2055..>2749
                     /locus_tag="ACH3L1_004445"
     CDS             2055..>2749
                     /locus_tag="ACH3L1_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFT4078893.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          842 a          596 c          539 g          772 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc
      721 gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc
      781 aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttcttcc aggcttccag
      841 caacgagttt tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat
      901 atgctcactg aatccctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc
      961 ctgctgtatc gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc
     1021 ccttgtaatt catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat
     1081 taatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt
     1141 atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac
     1201 accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa
     1261 ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg
     1321 aagctagcct gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta
     1381 gccatgatgt ttttgaacct tatcctgcgg cgttacatgg tcaatttgat tccatttcca
     1441 tgttttacct tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca
     1501 atgcggcgca ggccttaact gatgatggaa ctctatacgg agccacaatt cttggcgatg
     1561 gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct
     1621 tttcaaacac aaaagattcc gaagaaggct taacacatat actctcagag catttcgaga
     1681 atgttaaaac caaggttcaa ggtactgtag taatgttttc cgcttcaggg aaaaaatagc
     1741 atccaaccgc agcacgttct tgcttaagac gtgctacggt ttcctttacg atttggggtt
     1801 gcgactttac ctctattgat aatgcattcc ggcagaacgt tcaaatatga acgtacgata
     1861 tttaactaac cgaaaaacaa aatatagcac aggcgagaca tttaccatct aagaaaatag
     1921 tatcgttttt ttacagttaa atcaacatca cttccttaaa atgaaaacaa taaatttcaa
     1981 tcctgaaaca ttttatttca gcttattatt ccccagtatc acattttatc aggatatcct
     2041 tctgcgaggt tattatgctt cctgtaaata atccccccct atccactgga aacgtctctt
     2101 tttacagaac tacatcaatc gacaatgttc acaataatta tctctccgaa tgggttgaat
     2161 ggactaaaaa cagcatttcc ggagaaaaca gggaaactgc ttttacccgg ctccaattat
     2221 gtctggagaa cagtgaaaca tcgttggact tatcttgttt aggtctcaga tctctaccac
     2281 gattgcctga caatcttgat gaaattaatg taagcaataa ccaactatca atgctccccg
     2341 agctaccaag ggcattgaaa gagctgaatg caagcagtaa tcaattatct gcacttcctg
     2401 aattaccagt gtcgctggaa tatataaatg tgagtgataa ccatttgttc gcacttcctg
     2461 aattacctgc gtcactagaa tatattaatg taagtgacaa tcacctgtct gtacttccga
     2521 ggttaccaat gtcattggaa ttacttgatg cagccagaaa tgctttggaa gtaataccag
     2581 attttccaga aagagatgat catattataa gaatattctg gcttaatcag aaccggatca
     2641 cggcaattcc ggaaagcata cttggcctca gttctgatag cgttgtcaat cttagagaaa
     2701 atcaactatc tcccagaata atgcaaactt tgttacaaca aaccgccca
//
LOCUS       DAZXUL010000271         2715 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZXUL010000271 DAZXUL010000000
VERSION     DAZXUL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.272"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..224
                     /locus_tag="ACH3L1_004446"
     CDS             6..224
                     /locus_tag="ACH3L1_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFT4078894.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPIFPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="ACH3L1_004447"
     CDS             226..591
                     /locus_tag="ACH3L1_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFT4078895.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="ACH3L1_004448"
     CDS             588..1253
                     /locus_tag="ACH3L1_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078896.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="ACH3L1_004449"
     CDS             1253..1618
                     /locus_tag="ACH3L1_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFT4078897.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2073..2228
                     /gene="hokD"
                     /locus_tag="ACH3L1_004450"
     CDS             2073..2228
                     /gene="hokD"
                     /locus_tag="ACH3L1_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFT4078898.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            2504..>2715
                     /locus_tag="ACH3L1_004451"
     CDS             2504..>2715
                     /locus_tag="ACH3L1_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078899.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETPKSIMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          772 a          556 c          696 g          691 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatt cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     2161 gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg
     2221 aggagtaaga gaccaggcgg gggagaaatc cctcgccacc tctcatgtgt caggcatcct
     2281 caacgcaccc gcactaaacc cgcttcggcg ggttttttgt tgcgtgctga atacgcaggg
     2341 tgaaaaataa ccatatattt gattatatac acaacaaaaa ataaaagtca ttgtacttgc
     2401 acattaaaca atcaaatata cggcgtgaaa taaatattta tcagattaat atttttgtct
     2461 ctatgtggat ataaccgttt gtacttataa acctgggggc atcgtggaaa aaataaagaa
     2521 actatttagt agcaaatacg cagtcatacg tcgtgatgac ctgtcagtta tagtcgaaat
     2581 ggattacttc cctgaaaccc caaaatcaat aatgtatcgt aatggtcgaa aggcaatttt
     2641 tttaccgatg agggtaagtg acattatggg aaatgataaa ctgctggatg aattgcgagt
     2701 cagagcatcc tgtta
//
LOCUS       DAZXUL010000272         2713 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZXUL010000272 DAZXUL010000000
VERSION     DAZXUL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.273"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            50..2572
                     /gene="lpfC-O113"
                     /locus_tag="ACH3L1_004452"
     CDS             50..2572
                     /gene="lpfC-O113"
                     /locus_tag="ACH3L1_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFT4078900.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2583..>2713
                     /locus_tag="ACH3L1_004453"
     CDS             2583..>2713
                     /locus_tag="ACH3L1_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4078901.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     "
BASE COUNT          726 a          585 c          679 g          723 t
ORIGIN      
        1 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
       61 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      121 cgaggtcagt gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat
      181 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      241 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      301 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      361 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      421 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca
      481 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
      541 taatgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
      601 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
      661 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
      721 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
      781 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
      841 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
      901 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc
      961 acaaggtgct tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt
     1021 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1081 gattcttaaa cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1141 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1201 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1261 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1321 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga
     1381 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1441 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     1501 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     1561 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     1621 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     1681 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     1741 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     1801 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     1861 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     1921 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     1981 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2041 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2101 cgccaggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2161 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2221 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2281 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2341 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2401 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     2461 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc
     2521 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     2581 ttatgaacaa gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg
     2641 tagcacttgc agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc
     2701 ttgatttctc ggg
//
LOCUS       DAZXUL010000273         2637 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZXUL010000273 DAZXUL010000000
VERSION     DAZXUL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.274"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..159
                     /locus_tag="ACH3L1_004454"
                     /pseudo
     CDS             1..159
                     /locus_tag="ACH3L1_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003117273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(423..758)
                     /locus_tag="ACH3L1_004455"
     CDS             complement(423..758)
                     /locus_tag="ACH3L1_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078902.1"
                     /translation="MRTITTREQLLVNGKVRERIATHIVTGAHGYETLCTSGYNLQYN
                     KERVLIENCEKVADGELPVTCHTCFSIWQDVHRFKPGDFDTESGKGNFTDTELTKITI
                     GQEKTPNAC"
     gene            1096..2076
                     /locus_tag="ACH3L1_004456"
     CDS             1096..2076
                     /locus_tag="ACH3L1_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFT4078903.1"
                     /translation="MRIFVDDGSTNIKLAWVEDGAVKTLISPNSFKPEWSMTLQGLTD
                     ATVPASANYEIDGEKYSFDQLSPDAVRTTETRYQYSDVNVVAIHHALMQSGIAPQPID
                     IVVTLPLGEYLDENDQPNLANIERKKNNVRRTVTVQGRENFTIRKVSVLPESVPAGFD
                     VLKDLNDLESLLIVDIGGTTLDIAHVRSKMSGFTKTYCDPKTGVSIITEAVKHAMDVN
                     VSTRTSSYFADRLIQGRDDNEFLTRYIPNAEQRERILTVLRDREKKLQHRVADSVGRF
                     AGFTHVMVVGGGASLVAGAVKKATGVGDNRFFVSDNPQFDLVLGMMAMKG"
     gene            2079..2501
                     /locus_tag="ACH3L1_004457"
     CDS             2079..2501
                     /locus_tag="ACH3L1_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFT4078904.1"
                     /translation="MADNRKCSFYIYPERNAADRVADRFLEKLPQKERGRAMRAMMLC
                     GAALMKQDERLPFLIAEFLTDSTSMQDIQRIISSTLPQQENGEVVRLLEAFLQSAGNN
                     AKAILPAVDSATQEISAPVDQNLLETRNNIKNLFPDDE"
     gene            2505..>2637
                     /locus_tag="ACH3L1_004458"
     CDS             2505..>2637
                     /locus_tag="ACH3L1_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078905.1"
                     /translation="MNSVKGRCCVLFSFPTKNVAGLLFLLRRLTRRGRNAACGQCPAS
                     "
BASE COUNT          637 a          564 c          655 g          781 t
ORIGIN      
        1 gcctcgctga tgctcaggaa actcggcagc tacccgcgcc agaacggctt ggccgtcgcg
       61 ctgcgcgagt tgggccgcat cgagcgcacg ctgttcatcc tcgactggct gcaaagcgtc
      121 gagctacgcc gccgcgtgca tgccgggctg aacaaggggg cagtttggat atagagaatt
      181 attgtacggt aagtctgttt tttgaacaga tttacctatc gcgatagggt gtactcccgc
      241 gctatacgct gttttcaggg gatttgaact caatcggata acctgaatgc aataccgcaa
      301 tggcgataat ttaaaagcga tagccgttga cgcaaaatca atccgttacg catctctatt
      361 ggttaaataa tgcgcttaac gtacaaaatt ttcccatatc caaactgacc ccttaaagga
      421 catcaacacg cattcggcgt tttttcttgt ccaattgtga ttttcgtgag ctccgtatca
      481 gtgaaatttc cctttcctga ttctgtgtca aagtcaccag gtttgaaccg gtgtacatcc
      541 tgccatatag agaaacaggt gtggcaggtg acaggcagtt ctccatcagc caccttttcg
      601 cagttttcaa tcagtacccg ttccttattg tattgtaagt tataaccact tgtgcaaagc
      661 gtttcatacc cgtgagcgcc tgtcacgata tgtgtggcaa tccgttccct gacttttccg
      721 ttcactaata actgttcacg tgtggtgatc gttcgcattc tcttcctcat cagctttcct
      781 gtagaagcct cctgactata aggctccgga tatcttaccc gctcattttg gctggaggta
      841 gtggtggcgt cagaggatga attactgaca ttgtatatga agctttgagt tttatctgga
      901 ttttatcagt gtttatcttt atttgttctt gtttgttctc ttttgtgctg ttggagtcgg
      961 tttttgtctg tgtttttctt tatttttcca tttgtggccg atatcaggtc tttaccgata
     1021 cgttgtttta ggtgataatc gtttttgatc tcgtattttt ctaaaaaaat ccattttatt
     1081 tatgtgaggt tctagatgcg tatctttgta gacgacggct ctaccaacat caaactggcc
     1141 tgggttgaag acggtgcagt gaaaacgtta attagtccga atagttttaa gccggaatgg
     1201 tctatgacgc tgcagggact gactgatgcc actgtacccg cttccgcaaa ctatgagatt
     1261 gatggagaaa aatacagctt tgatcaattg agtccggatg cagtgcgcac cacagaaacg
     1321 cgttatcagt acagcgacgt taatgtggtt gctattcatc atgcgttgat gcagtcaggt
     1381 attgctccac aacctattga tattgttgtg actttacctt taggtgagta tctggatgag
     1441 aacgatcagc caaatctggc taatatcgaa cgtaagaaaa ataatgtccg gcgaactgta
     1501 acggttcagg ggcgtgaaaa ctttaccatt cgtaaagtca gtgtcttgcc tgagtccgtg
     1561 cctgccggtt ttgacgtgct gaaagatctg aatgatctgg agtcgctgtt gattgttgat
     1621 atcggtggaa cgacccttga tattgcacat gtccgcagca aaatgtcagg tttcacaaaa
     1681 acctattgtg accccaagac tggcgtctcc atcattactg aagcagttaa gcatgcgatg
     1741 gatgtgaatg tgagtacacg aaccagttct tattttgcag atcgtctaat ccaggggcgg
     1801 gatgacaatg aattcctgac ccgttatatc ccgaatgcag aacagcgtga gcggatcctg
     1861 acagtattac gtgaccggga aaaaaaactg cagcatcgtg tggcggattc agtaggacgc
     1921 tttgctggtt ttactcatgt tatggttgtt ggcggtggtg ccagccttgt ggctggggca
     1981 gtaaaaaaag cgacaggagt gggtgataac cgtttctttg tcagtgacaa tcctcagttc
     2041 gatctggtgt tgggaatgat ggcaatgaag ggatgagtat ggctgataat cgtaagtgct
     2101 ctttttatat ttatccggaa cgaaatgcag ctgatcgggt ggctgacagg tttcttgaaa
     2161 aattgcctca gaaagaacgt ggtcgggcaa tgcgggccat gatgttgtgt ggtgctgcat
     2221 tgatgaagca ggatgaacga ttgccgttcc tgatagctga attcctgaca gacagcacgt
     2281 ctatgcagga tattcaacga attatcagta gtacgctacc acaacaggaa aatggtgaag
     2341 tcgttcgttt gctggaggca tttctgcagt ctgctggtaa taatgcaaaa gcgatcctgc
     2401 ctgcggttga ttctgctacg caagaaatat ctgctccagt cgatcagaat ctcctggaaa
     2461 caagaaacaa cattaagaac ttgtttccgg atgatgaata agaactgaac tctgttaaag
     2521 gacgctgttg cgtccttttt tcttttccga caaagaatgt cgccgggctc ctctttttac
     2581 tgcgccggct gacgcggcgc ggcaggaacg ctgcctgtgg tcagtgtccc gcgtccg
//
LOCUS       DAZXUL010000274         2573 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZXUL010000274 DAZXUL010000000
VERSION     DAZXUL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.275"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            27..278
                     /locus_tag="ACH3L1_004459"
     CDS             27..278
                     /locus_tag="ACH3L1_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFT4078906.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            280..2376
                     /locus_tag="ACH3L1_004460"
     CDS             280..2376
                     /locus_tag="ACH3L1_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFT4078907.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT          706 a          594 c          721 g          552 t
ORIGIN      
        1 gggtaatttt gtaggttaaa ggaaagatga gacaaagaga agatagtgac cgcgattggc
       61 cttcgctgaa gattcagctg gcgctcaaag ataacgggtg gtcttccttc cgggagcttt
      121 cactccatgc tggttatgct caggattcac tgaaagcggt agttcgcaca ccaaacaaaa
      181 aatacgaacg cgtaatttct gacgctatag gtgtaccgcc agaaatcatc tggcccagcc
      241 ggtatcgtaa cgatggattt atcaggaagg cgtcgtgata tgcagtcgta cgttaccgta
      301 aatgacctgc tcggcgtacc aggaatgcca gccaccacaa aaggcatccg acaggcgtta
      361 cagcgttttt ctcgtgatct tggtgacgtt agtcgccgtc gggaaggcac aaaagccatt
      421 gagtaccaca ttgattgtct gcctgaaata acacgaaagg cactgcgtga acgctatgtg
      481 gaacagctgg tggctacaga gaacaatgtt tctgaagtga aagctgttac cagaaaaaca
      541 cgcaatcctg acgctgtcca ggcaatcgaa gcatatcgcg gttctccaca actgatggaa
      601 gaacgcctga atgcgctgac cgaaaaccag cgctgggtat ctgaagcaag agctgcgctg
      661 gtggtggaag tgctgaagct ggaaagcgcc ggtaaccccg ggcgactgaa agccattaac
      721 tttcttgttg aaaaagcccg taaaggtgag ctgccggagc gcctgcaaca ggccgcagtt
      781 aacgccaatg caaaacgtgg cgctaatcgt actatcagcc gcgatccact ttaccagtgg
      841 gttctgaaat acaaccagtc gcagaacgcc gccgaacggc tgctgttact ggcaccggga
      901 aaacgcgacg aaataaaacc ggaagaaatc agctggctgc ctgaatttct ggcgcagtat
      961 cgccaggtga acggcagacc catgtcagaa gcctacgagg attttgtcgc agaatggcag
     1021 cgtcgccatg cggacgaacc gtacatgctg gaggtgatgc cttcctatga tgttgtccgg
     1081 tatgccatga aaaaactgcc ggaagtggtg aaacaaaaag ggcgcgttac cggcagtgaa
     1141 taccgtcagc ttgaaggatt cacccgccgc gactggacag ctatgccggt gaattatgtc
     1201 tggattggtg acggtcacgg tatgaagctg aaatgtgcgc atccgatcca cgggcgtcca
     1261 ttctcaccgg aagtcacgtt tgtgatcgat ggtggaacac ggtttgttgt tggctggagt
     1321 cttgaccttg ccgaaaacgt gttcgccgtt gccggtgcga tacagcacgg cattcgcaac
     1381 cacggaaaac cgttcctgta ttactcggat aacggttccg gtgaaacggc tgacatgctg
     1441 gacaaagagg tcgtgggtat tctgccacgc ctggggatta aacaccctac cggtattgcc
     1501 ggtaatccac agggacgcgg cattattgaa cgcctgaacc ggacactgcc gatgcgtata
     1561 gccaggaggt atcgcaccta ttttggcaag ggtgctgacc gggaatcgtt gcgtgtactt
     1621 aaccgtgatc tgcgttcagc gtttaacgcc ctgcaacagg acaagcctct gaacgaccgc
     1681 cagaaagcgg caatgcgcga gctgccatcg tgggcggagc ttattgaggc tattcgtgaa
     1741 ggcgtggaat ggtacaacaa ccgcccgcat tctgagctgc cgatgaaacc gaacggacgg
     1801 cattacagcc cgacagagtt cagaaaaaaa cgccaggcag aagaagacac cgaaattgag
     1861 tggctgagtg atcttgagct gcgcgacatg ttccgcccaa tggtggaacg accggtaaga
     1921 cgctgcgaaa ttcagtggct gaacaatatt tactacgcgc cggagttgcg tgacgaacac
     1981 ggtcgcaaag tgcttatcag ttatgacatt catgacgccg agcgaattac ggtacgtcgc
     2041 aaggacggca gctttatctg tgaggcgata tggaacggca ataaacgcgc agcctttgct
     2101 gtcagtgctg aatatcacaa acaacagcag cgtattaaag gaatgcgcaa acgcgctgag
     2161 gaaaaaattc gtgatgctga agatgaaggt attcagattc tggaacacaa acaggctgaa
     2221 ccctggctga gtaatgttta tcgcccggtt ggtaatgtgg tggcagttca gcaaccggaa
     2281 tatgaagaag agcatgacga agaatttgaa cgcgatttcc ggctggggat gcaaaaactt
     2341 tttgccatgc aggaagaaga tgatccgctg gcctgaataa aaaaacagtc cgcgggaacg
     2401 gactgttaaa taaaggaaac aaagctgatt tgaaataacc gaggaaatac tatgactgat
     2461 attgatgatg tttgaggtgt actggcaata gcggacacta ccatttgttc tttttttaag
     2521 cagccatctg atgatatttt tccctgaagg ctgccgggga gataggggag ata
//
LOCUS       DAZXUL010000275         2566 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZXUL010000275 DAZXUL010000000
VERSION     DAZXUL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.276"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..489
                     /gene="capU"
                     /locus_tag="ACH3L1_004461"
     CDS             <1..489
                     /gene="capU"
                     /locus_tag="ACH3L1_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFT4078908.1"
                     /translation="EFMLNLLFHLKMNGRQFCWLIVGSGSPELREHLQYQIDSMGMHD
                     DVFIADNVFPAAPVYRVASLVVLPSENESFGMVLAEASAFSVPVVATQIGGIPEVIQN
                     NQTGTLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAK
                     QK"
     gene            494..1444
                     /gene="virK"
                     /locus_tag="ACH3L1_004462"
     CDS             494..1444
                     /gene="virK"
                     /locus_tag="ACH3L1_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFT4078909.1"
                     /translation="MFSTSNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSGYRKRYALLDHIHETTIRSLDAYPVHSEHQNLN"
     gene            1509..2453
                     /gene="lpxM"
                     /locus_tag="ACH3L1_004463"
                     /gene_synonym="msbB"
     CDS             1509..2453
                     /gene="lpxM"
                     /locus_tag="ACH3L1_004463"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFT4078910.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          740 a          490 c          583 g          753 t
ORIGIN      
        1 gaatttatgt tgaatttact gttccattta aaaatgaatg gacgacagtt ctgttggttg
       61 attgtggggt ctggttcacc tgaactgcgg gaacatttac agtatcagat tgacagtatg
      121 ggcatgcatg atgatgtttt tattgctgac aatgtttttc ctgccgcccc cgtatatcgg
      181 gttgccagtc tggtggttct gccttcagaa aacgaatctt ttggtatggt gctggcagaa
      241 gcatcggcat tttctgtgcc tgtagtggcc actcagattg gtggaatccc tgaggttatt
      301 cagaacaacc agaccgggac attgttacca gcaagtaata agcacgcatg gatgtgcgcc
      361 ctgaatgatt tttttaatga ccctgggcgt ttttatcaga tggctcgcct ggcaaaacag
      421 gatatagaag agcggtttga tattaataaa actgcgttaa aaatactcac attagcgaag
      481 caaaagtaac aatatgtttt ctacaagtaa cttatcattt atcggtttcc ttaaaaggat
      541 tattttttcc tcagattcac taccggggaa gtgggaacac agaaaatttc ggttcatgta
      601 cattttgcga tgttctataa atccggttgt cagtattcga tattattatg aactgcgttc
      661 cttgccgtgc attgaggata ttctggcaat acaccccacg ttgccagcaa gaattcatcg
      721 cccttatttg cataaagggg ggcgcgcctg gacccgtgga caatatattc ttgagcatta
      781 tcgctttgtg caaaatttgc cagaaaaata ttctaaattt ttgtttccac aaaaatcggt
      841 atcattagtc caattcatcg ggaaagatgg agagaatttt gatatccagt gttctcccag
      901 tggttttgac cgggaaggag agttgatgtt gtcattgttt tacaataaaa cagttattgc
      961 ccggttaaca ttttcagtca ttctgactca aaatggacac attgctttta ttggcggatt
     1021 acagggagcc cctaaaaata ccggacctga tgttattcgg tgcgcaaccc gggcctgtta
     1081 tgggattttc ccaaaacgga ttatttttga agcattttgt gcgttgatga aagcttgcaa
     1141 tatctctgag tgtctggcag tcagtgagca cagtcatgtt ttccggcaat tgagatactg
     1201 gtatcagaaa cgcaaaacct ttgttgcggt ctatagtgat ttctgggagt ctgtagcagg
     1261 aaaaacctgt ggtgactggt acagattacc aacccaggtg attcgaaagc cgctaagcga
     1321 tatagccagt aaaaaacgct ctgggtaccg aaaacgatac gctttgctgg atcatatcca
     1381 tgaaactact atccgctctt tggatgcata tcctgtacac tcagaacatc agaatttaaa
     1441 ttaaggtttg atgtattctc tgaataaaac agtaatgata attttggtag aggcattcgc
     1501 actaaataat gaaaaaatat aaatctgagt ttattcctga atttaagaaa aattaccttt
     1561 cccctgttta ctggtctaca tggttccttt tgggaatgat tgcaggtatt tcaatgtttc
     1621 ccccttcatt cagagatcct gtcttggcaa aaatagggcg ttgggcgggc agattgagca
     1681 aaaaagctcg tcgcagggcg acgattaatt tatcgctttg tttcccagaa aagagtgata
     1741 cagaacggga aataattgtc gacaaaatgt ttgtcacagc attgcaatcc atagtgatga
     1801 tggcagaact ggcaattcgt ggtccggaaa agttccagaa acgagtgttc tggaaaggcc
     1861 tcgaaattct tgaggaaatt cgacacaata atagaaatgt gatttttctg gttccccatg
     1921 gctggagcgt ggatattcct gcaatgttgc tggcagctca gggaaaaaaa atggccgcca
     1981 tgtttcatca gcaacgaaat ccagtgattg attatgtctg gaattcagta cggcgtaaat
     2041 tcggggggcg cttacatgcc cgggaggatg ggataaaacc atttattcag tcagtacgcc
     2101 agggatactg gggatattac ctcccagatc aggatcatgg tcctgaatac agtgaatttg
     2161 ctgatttttt tgcgacctat aaagcgacat taccaatcat tggacgtctg atgaacatca
     2221 gtcaggctat gattatacca cttttcccgg tttatgatga aaaaaaacat ctactgacta
     2281 ttgaaattcg gccaccaatg gatgcttgca ttgccagcgc ggacaataaa acgattgccc
     2341 gacaaatgaa caaaacagtg gaaattttgg tggggccaca tccggaacag tatgtctggg
     2401 ttttaaaatt gttaaaaaca cgcaaatcaa acgaagcgga cccgtaccct tgaaattata
     2461 aagtacaagt atttccacta gttgtttgta agcgtacagc ctgaaccgtc tggtcagaat
     2521 ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaac
//
LOCUS       DAZXUL010000276         2529 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZXUL010000276 DAZXUL010000000
VERSION     DAZXUL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.277"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(133..453)
                     /gene="psiF"
                     /locus_tag="ACH3L1_004464"
     CDS             complement(133..453)
                     /gene="psiF"
                     /locus_tag="ACH3L1_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFT4078911.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(572..1987)
                     /gene="phoA"
                     /locus_tag="ACH3L1_004465"
     CDS             complement(572..1987)
                     /gene="phoA"
                     /locus_tag="ACH3L1_004465"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFT4078912.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2088..2348)
                     /gene="iraP"
                     /locus_tag="ACH3L1_004466"
     CDS             complement(2088..2348)
                     /gene="iraP"
                     /locus_tag="ACH3L1_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFT4078913.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
BASE COUNT          587 a          648 c          588 g          706 t
ORIGIN      
        1 ggaaaggctg ggccacatcc ctccggcaga agcagaaaaa gcttattatg cttccatcgg
       61 aaacgatgat ctggcagcct gagttcacag ataaaacact ctccaggaaa cccggggcgg
      121 ttcaatcagg tatcaggcgg ctttcttgag gcaggcactc ataaacttat tacgatcatc
      181 acctttcaga gcttgttgcg ttgcttgatt attacattca cgcatctttt gctgctgtgg
      241 cgtcaaactt ttttcaccag gcgcagactt gctgttcttc aggcaatcac tcatgtaggt
      301 cttacgagca tcccctttca acgcctgcgc cgtcgcctgc tgattacagg aggtcatacg
      361 ctgttgttgt ggggttaagg ttctctcggc agcgccgacg gtggttaaaa aaaccagacc
      421 aaaaagcaag gtaaccagta atgttatttt catagcacca tccctcttca tgttttaacc
      481 atgagcgttt gcgcccgtga tctgccatta agtctggttg ctaacagcaa aaaaaccacc
      541 cggcagcgaa aattcactgc cgggcgcagt tttatttcag ccccagagcg gctttcatgg
      601 tgtagaagag atcggtctgg tcggtcagtc caacaacatt ggcggcatgc gggccatacg
      661 ccgcaatacg caactgactg ccggtatgtt cttgtgaatc ctcttcggaa ttcccgtaac
      721 tcatcaccat cactgcgcca tctttagtat ttagcgcctg agtgaggccc ggcgctttgg
      781 tatccggcgc aacaacctgg ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt
      841 taccctcctt tttagcgaat tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct
      901 cgccaatttg cccacaaggg ttcgcagcgt gatcctgttt atcgattgac gcaccttcaa
      961 cttgcaggaa aaatcctttc tcatttttac tcaacaattc aatggctttg tcggtcatct
     1021 gtgccagggt tggtacactg tcattacgtt gcggattagg cgtataggtg actgcgggct
     1081 tatcgatatt gccgtggtac gttgctttcg gccctagcca gcgcactggc atattgccgt
     1141 cagcaaacag gccaagcagg ggtttttgct gattcgcttc cgtcaccgaa ttcagtgagg
     1201 cagcatcgtt caccaactga taaccacgcg cctgtgcctg ttcacgcagc gtttttccct
     1261 gccattcacc agcggttgcc gtttcagcaa aggtttttgc gccgccgcca agcgtaacgt
     1321 cggcacgagc gttaagcagc tgttcggtaa tcgatccttt tccgcctttt tccagagcgt
     1381 tacccggaca tttttcactg gtcgcgctcg gaccgtagca tttgcgcgaa gtcacatgtg
     1441 ccaccagcgc agcgggcgtg gcatcctgca actctgcggt agaaacgtta ccggtcgcca
     1501 gacctgcggc ttttgccatt tccagaatcg ttgggtgatc tttttcgtga atatcgacgc
     1561 ccagcgcgcc gttataggtt ttgacatcgg ttgaccaggc ggttgctgat gcagccgagt
     1621 cggtgacgta gtccggtttg ccagtttttt tattcagcgc atagtgagtg tattgcccgg
     1681 taagcggtaa ggcatcgata cctttaaaaa agccgcccgc accttcggca taattacgtg
     1741 cggcagtaat ttccgaatcc cccatcccat cgccaatcag caaaataata ttttttgcag
     1801 gtttatcgct aagagaatca cgcagagcgg cggtctgatc acccgttaag cggcgagcac
     1861 cgccgggtgc agtaatatcg ccctgagcag cccggttttc cagaacaggc atttctggtg
     1921 tccgggcttt tgtcacaggg gtaaacagta acggtaagag tgccagtgca atagtgcttt
     1981 gtttcacttt attttctcca tgtacaaata cattaaaaaa taaaaacaaa gcgactataa
     2041 gtctcggccg tgacaacttt atgacagctg ttgaaaagat taacttttta ctgacaagga
     2101 tgcttcaata acttctttac gtaatcgcgc agcagctctg tatcgtcgtc aggaatgctg
     2161 gcatcgggct ttacctcgta cagcgccccc tctacctgat caatcaaccg ctgttggtca
     2221 ttttgcgcca tattgcgaag cattgcagtg acgataatct ccaaagcttc tacctgcgca
     2281 cacagttctt tcgactcttc ttctttttgg gcaagcttaa ataacaactc agcaatgaga
     2341 tttttcatgt ctgtatttcc ttatccaaag tatggagaag ttaacattat gtttcacaac
     2401 ttcatagtgt ctggaagtat tatttttatg acaaaggaaa tactttgcgc aaatattttt
     2461 tgtttgattg ccagcgaaac gtttctctta tttaaataat tacccacaca aaatataggc
     2521 ttttaatta
//
LOCUS       DAZXUL010000277         2526 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZXUL010000277 DAZXUL010000000
VERSION     DAZXUL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.278"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            95..1210
                     /gene="adrA"
                     /locus_tag="ACH3L1_004467"
                     /gene_synonym="dgcC"
     CDS             95..1210
                     /gene="adrA"
                     /locus_tag="ACH3L1_004467"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFT4078914.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1227..2036)
                     /gene="proC"
                     /locus_tag="ACH3L1_004468"
     CDS             complement(1227..2036)
                     /gene="proC"
                     /locus_tag="ACH3L1_004468"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFT4078915.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2156..>2526
                     /locus_tag="ACH3L1_004469"
     CDS             2156..>2526
                     /locus_tag="ACH3L1_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFT4078916.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGG"
BASE COUNT          557 a          643 c          710 g          616 t
ORIGIN      
        1 tagtgctaac gggtgagcta cgaaagtggc tcacccgaaa tttcatactt ctgcctttag
       61 ccccgtctct ataatttggg aaaattgttt ctgaatgttc ccaaaaataa tgaatgatga
      121 aaactttttc aaaaaagcgg cggcgcatgg ggaggaacct cctttaactc ctcaaaacga
      181 acatcagcgg tccgggctgc gcttcgcccg tcgcgtcaga ctaccccgtg cggttggcct
      241 ggctggcatg ttcttaccga ttgcttcaac gctggtttcg cacccgccgc cgggctggtg
      301 gtggctggtg ttggtcggct gggcgttcgt ctggccgcat ttagcctggc agatagcgag
      361 cagggccgtc gatccgctta gccgggaaat ttacaactta aaaaccgatg cagtattagc
      421 gggaatgtgg gtaggcgtaa tgggcgtaaa cgtgctgcct tccaccgcta tgttgatgat
      481 tctgtgtctg aatttgatgg gggcaggcgg cccccgtctg tttgtcgcgg gtctggtgtt
      541 gatggtggtt tcctgccttg tcaccctcga gcttacgggc attaccgtgt cgttcaatag
      601 tgctccgctg gaatggtggc tctcccttcc cattattgtc gtttatcctc tgatgtttgg
      661 ctgggtcagc taccagacgg caaccaaact ggcggaacat aaacgcaggt tgcaggtcat
      721 gagtactcgc gacggcatga cgggcgtgta taaccgacgt cattgggaaa ctatgttacg
      781 caatgaattt gataactgtc ggcggcataa tcgcgatgca acgttactga ttatcgatat
      841 cgaccatttc aagagcatca acgatacctg gggtcatgat gtgggcgatg aagcgattgt
      901 ggcgcttacc cgacagttac aaattaccct gcgcggtagc gatgtgattg gtcggtttgg
      961 cggcgatgag tttgcagtaa tcatgtccgg tacgccagct gagagcgcca ttaccgccat
     1021 gttacgggtg cgtgaagggc taaacacgtt acgcttgccg aatacgccac aggtaacttt
     1081 acggattagt gtgggggttg cgccgctgaa cccacaaatg agtcactatc gtgagtggtt
     1141 gaaatcggca gatttggcgc tttacaaagc aaagaaagcc ggacgtaacc gcaccgaagt
     1201 ggcggcctga cgtccggcga aagtcatcag gatttgctga gtttttctga tttttccata
     1261 cacttcgtca tcgcttcgat cactgcggca cggaagcctt tctcttccag tacgcgtacc
     1321 gcttcaatgg tggtgcctcc cggtgagcag accatatctt tcagtgcccc cggatgttct
     1381 cccgtttcca gcaccatttt tgcggaaccc attaccgcct gagcggcaaa tttatacgcc
     1441 tgggcgcgtg gcatcccgcc cagcacggcg gcgtcggcca tcgcttcgat aaacataaat
     1501 acgtaggctg gcgaagaacc gctcacaccg accaccgggt ggatcatcgg ctcagcaatt
     1561 acttccgctt cgccaaagca gcggaaaata ttcagcacat cagcggtatc ttctggggtt
     1621 accagcgcgt ttggcgttac ggaggtcatc ccggcattaa ccagcgcggg agtgttcggc
     1681 atggcgcgga taattttccg gtcatggccc agcgcgcggg caagctggtc gagcgtgaca
     1741 cctgcagcaa tagaaacgac cagagagtct ttattcaggc tggaggtgat ttcgctaagc
     1801 actttaatca tgatgccagg tttaacggca gcaaaaatga tgtcggcgat ttgcgccact
     1861 tcttgcgccg attctgcggc gttgatgccg aactggtcat gcagggcggc gactttatcc
     1921 ggggaggggg tgtataccca gatttgccct ggaagcacct gaccgctggc aatcagaccg
     1981 ccgagaatgg cttttcccat attgccgcag ccaataaaac cgattttctt ttccattgcc
     2041 tcactcctgc cgtgaaattc attgttttga taatcgctgg cagaagcata aacagaacta
     2101 tgccggaagg caaaagcgcg acacaataga ggattaccta acaaaggatg actttatgac
     2161 aatttgggtg gatgccgacg cgtgtcccaa tgtaattaaa gagattttgt atcgcgcggc
     2221 ggaacgtatg cagatgccgc tggtactggt agcaaaccag agtttacgcg tgccgccatc
     2281 gcgatttatt cgtacgctgc gcgtcgcggc aggtttcgac gttgccgata acgaaattgt
     2341 ccggcagtgt gaagcgggcg atttggtgat caccgcagat atacctttgg ctgctgaagc
     2401 catcgagaaa ggcgcagcgg cgcttaattc gcgcggcgaa cgttacacgc cagcgaccat
     2461 tcgtgagcgc ctgacgatgc gcgattttat ggatacctta cgtgccagtg ggatccagac
     2521 cggcgg
//
LOCUS       DAZXUL010000278         2491 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZXUL010000278 DAZXUL010000000
VERSION     DAZXUL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.279"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            262..1215
                     /gene="lsrR"
                     /locus_tag="ACH3L1_004470"
     CDS             262..1215
                     /gene="lsrR"
                     /locus_tag="ACH3L1_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFT4078917.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            1294..2121
                     /locus_tag="ACH3L1_004471"
                     /pseudo
     CDS             1294..2121
                     /locus_tag="ACH3L1_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
BASE COUNT          618 a          569 c          664 g          640 t
ORIGIN      
        1 cactcgtttg catatttccc ccgttcagtt ttgcaggtga gttttgaaca aatgtatttc
       61 tacttttaat ttgttcataa ccttaggtgg acattgcaca tatttccgac gaatagatca
      121 caatttatgc tattttgatt ttcacggttg cgtttgttca tgctcgtaga gtcaaactgt
      181 ggttgccatc acagatataa atgcgcaaga actgaacaat tgcattaaag atttaaatat
      241 gttcaaagtg aagaatgaat tatgacaatc aacgattcgg caatttcaga acagggaatg
      301 tgtgaagaag aacaggtcgc gcggatcgcg tggttttact atcacgacgg gctgacccag
      361 agcgagatca gcgatcgtct cggcctgacc cgtttgaaag tgtcgcgctt gctggagaaa
      421 gggcatcagt ccggcattat tcgcgtacag attaattctc gctttgaagg ctgtctggaa
      481 tatgaaactc aattacgtcg tcagttttcg ctgcaacatg tccgggtgat ccctgggctt
      541 gcggatgctg atgtcggcgg gcgactaggg ataggcgcgg cgcatatgtt gatgagttta
      601 cttcaaccac aacagatgct ggcgattggt tttggcgagg caaccatgaa tacgctgcaa
      661 cgcttaagtg gttttatttc gtcacagcaa attcgcctgg tcacgctctc cggtggcgtc
      721 ggttcttata tgacgggtat cgggcagctt aacgcggcgt gcagtgtgaa tattattccg
      781 gctccgttgc gggcatcctc cgctgacatt gcccgtacgc taaaaaatga aaattgcgtc
      841 aaagatgttc tgctagccgc gcaagcagcg gatgtggcga ttgtcggcat tggagccgtg
      901 agtcaacaag acgacgcgac aatcattcgc tccggttata tcagccaggg cgaacagtta
      961 atgattggcc gaaaaggggc ggttggcgac attttaggct acttttttga tgcaaaaggt
     1021 gacgttgtca cggatatcaa aatacataac gaactgattg gcttaccttt aagcgcgctg
     1081 aagaccatac ccgtccgggt tggcgtggca gggggagaaa ataaagctga agcaattgcc
     1141 gctgcaatga aaggcggtta tatcaacgca ctggttaccg atcaggacac agcagcggcg
     1201 attttacgta gttaaatttg catgacctta ttaccgggaa tgatggagaa cgtatagagg
     1261 aacagacaga acgcataagc cgaggataat ctaatggctc gactctttac cccttcagaa
     1321 tcaaagtact acctgatggc gctggatgca ggcaccggaa gtattcgggc tgtgatattc
     1381 gacctggaag gcaatcaaat agcagtggga caggcggagt ggcggcatct ggcagtaccg
     1441 gacgttcctg gttctatgga atttgatctc aacaaaaact ggcaactggc gtgtgagtgt
     1501 atgcgccagg cgctgcacaa cgccggcata gacccggagt atatcgctgc cgtttcggca
     1561 tgttctatgc gtgaaggcat tgttttatat aacaatgaag gaacaccgat ctgggcctgc
     1621 gccaatgtgg atgccagagc ggcacgcgaa gttagcgaac ttaaagaact gcacaacaat
     1681 acctttgaaa acgaagttta tcgagcgacc ggacaaacac tggctttaag tgccatcccc
     1741 agattacttt ggctggcgca ccatcgttcc gatatttacc gtcaggcatc aaccatcacc
     1801 atgatcagcg actggctggc ctatatgctc agcggcgaac tggcggtgga tccctctaac
     1861 gctggcacca cgggacttct tgatctaacc acccgtgcct ggaaacctgc attgctggat
     1921 atggctggcc tgcgtgccga tattctttct cctgtcaaag aaaccggcac attgctgggc
     1981 gtggtaagtt cacaagcggc ggaactctgc ggtctgaagg cgggcactct ggtggtcgtt
     2041 ggaggaggcg acgtgcagct tggttgcctt gggttaggcg ttgtgcgtcc ggcacaaacc
     2101 gcggttcttg gcggcacgtt cacggataat aagcagtgca cgctcagcga ggcccggcat
     2161 cagttgccga ttactccgtt ccgtatgccg tcatgagtcc catcctttga aagcagtgat
     2221 cgctgtggtg agactgtagc gcggacatgt ctatttagtc agagacggac atctcaattt
     2281 agctactaca atattactac gcataatgta tattatgtta aatcatgtct gagtgatgcg
     2341 aagtgctttt ctggtagtcg ttattcgttc agggttcaag aacttaagcc tctcctcttt
     2401 cataattggc ctgtgcatgg agaaatagcc tggttattcc ctgccccatg ctaagagcgc
     2461 agtgtctgtc gcatagacac tgcatacgaa a
//
LOCUS       DAZXUL010000279         2491 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZXUL010000279 DAZXUL010000000
VERSION     DAZXUL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.280"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>2491
                     /locus_tag="ACH3L1_004472"
     CDS             <1..>2491
                     /locus_tag="ACH3L1_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFT4078918.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQEQ"
BASE COUNT          730 a          578 c          602 g          581 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggagcaa t
//
LOCUS       DAZXUL010000280         2425 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZXUL010000280 DAZXUL010000000
VERSION     DAZXUL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.281"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1041)
                     /gene="ipaH7.8"
                     /locus_tag="ACH3L1_004473"
     CDS             complement(<1..1041)
                     /gene="ipaH7.8"
                     /locus_tag="ACH3L1_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HFT4078919.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGPQIYFSMSDGQQNTLHRPLAD
                     AVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLE
                     KLSASAELRQQSF"
     gene            1370..1549
                     /locus_tag="ACH3L1_004474"
                     /pseudo
     CDS             1370..1549
                     /locus_tag="ACH3L1_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1566..2256)
                     /locus_tag="ACH3L1_004475"
                     /pseudo
     CDS             complement(1566..2256)
                     /locus_tag="ACH3L1_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2335..2425
                     /locus_tag="ACH3L1_004476"
                     /pseudo
     CDS             2335..2425
                     /locus_tag="ACH3L1_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          696 a          457 c          574 g          698 t
ORIGIN      
        1 gaaagactgc tgtcgaagct ccgcagaggc actgagtttt tccagccatg cagcgacctg
       61 ttcacggaat ccggaggtat tgcgtgcaga gacggtatcg gaaaggcggt caaggaacgc
      121 ggaaaaggtg ttggcatgct cttcatgttc aaaagcatgc catatctgtg atacatcaga
      181 ttgtttgttt tccgggaacc atgctgtcac ggcatcagcc agggggcgat ggagtgtatt
      241 ctgttgtccg tcactcatgg agaagtaaat ctgtgggccg tggtagtccg gcgaagaggt
      301 taatctttgc agggattgca gaacgcgagt cgacaatgga ttccctgaaa tattaatcct
      361 gtaacttgaa tctaattgtg aaaaactctc cggaagagtg gttatctgat taaaatgaaa
      421 ataatattcc ttttgtctga tattgtttct atcagaaaat tgaggaaaag taaccagatt
      481 attgcgctga gcacagagaa tttccagttt atccggtaag tcgggtaatg ttgtcagcct
      541 gttataataa acatgtaaaa atttcaattt ttcgggaaga gccggtaagt tatatagcct
      601 gttattttca acaaataatg atgttaaatg gtttggcaaa acaggaaggt tttccagttg
      661 attagaacga acacttaaag ttgttaatga ctcaggaagt tctggtatca cagaaagcct
      721 gttattatca gcattaagtt ctttaagaaa tgcaggcagc tttggcaatg atgttaattt
      781 attattttct acaatcaaag cagaaatatg ctttggtaat tcgggaagag acgtcaaatt
      841 taggtctgat aaatttaata ctgcttcttg attctgaaag cactgggaga gtctgttaaa
      901 agcaatgcct cgctcttccc cgggagaaga gttcttttcc cattcagtca ggattctcag
      961 ataatgttca ttactggttg agtttgagtt aatagagggg gagcaagaaa ctgatgagtg
     1021 tgtattattt acagagaaca taatgctagg tcaaccttat atttgtgaga ataaatattt
     1081 aaaattgtat atttatacaa atttcactta ccatttccga tattaagttt taatagtttc
     1141 cattttacac ataacagaag ttagttgtaa tttatttatg gaggttttct atggaattct
     1201 ttttttattg tggctgcagt caataataac actgaattca acattccagt gagcaaggaa
     1261 atgtaagccg agtaagtata tttgttttac atcatatttt agtcaagctc atttattcct
     1321 attatagaat gacaaattaa aattactgta aaaaaacgat acacatttaa tacgagggag
     1381 gccggcagaa aaacggcttg cggtcaggca ggagaaatgt gtgccgcgaa tgaagtcctt
     1441 gtatgactgg ataaagctcc agaggaaaac tttgtcaaac catgctgaaa tggtaaaggc
     1501 gttcaactat atcctgaatc actggaatgc actgaatgag ttttgccggg tagtacttcc
     1561 aatcattaga atatgtgctt ttcaatgaag gaaccgatga ctttttcgtg gatttccacg
     1621 gaacgcgaga agcagattgt tttacgtgcc agccgcttga tgcgggggcg gagggtcagg
     1681 ttgttacgtt catacgttgc gtgaagattt tgccggtcag gtgtttctcc tccggcactt
     1741 ctctggcgta gctgccccag tcatcgctgg tcaccatgcc gatacagaac ggagtcaaca
     1801 gggctaacag ttcccgacag gtctcatcat tccgtggccc gcaagtgtaa gccaacacac
     1861 cgccagtttt agtgttgtac gcataccagg gccagtgttg ttgcgccttg ctgccaacga
     1921 agcgccactg ctcatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag
     1981 atgttatccg ccggggcgag agctttttaa tgtccgtatg acggtattga tgccgatttt
     2041 cagtgtcctg gcggtatcac ggccccctgc gccgttatgt gccatttcaa cgatctgctc
     2101 cttaacgccg agttttctgg cttcataaga gtaagtgagc tggaaaacac gtttacatga
     2161 gcgacaacgg aaacgctcat gccgggaaca actacggcca tgtcgataaa cttcatcgga
     2221 attacagcgc ggacaatgaa cagtaacggt agtcatggaa aacctcaaat accagtagat
     2281 taccagaaac ttctatcgat tgaagacatt accttttgcc gcaataggtg gggtaacatt
     2341 ggtgaaaacg cattacgccg ggtggcagtt ggaaaaaaat tatctttttt cggctctgat
     2401 cgtggtggag aaaatgcggc aataa
//
LOCUS       DAZXUL010000281         2372 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZXUL010000281 DAZXUL010000000
VERSION     DAZXUL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.282"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..823)
                     /locus_tag="ACH3L1_004477"
     CDS             complement(<1..823)
                     /locus_tag="ACH3L1_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFT4078920.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1423..1671)
                     /locus_tag="ACH3L1_004478"
     CDS             complement(1423..1671)
                     /locus_tag="ACH3L1_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFT4078921.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1934..2029)
                     /locus_tag="ACH3L1_004479"
                     /pseudo
     CDS             complement(1934..2029)
                     /locus_tag="ACH3L1_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2026..>2372)
                     /locus_tag="ACH3L1_004480"
     CDS             complement(2026..>2372)
                     /locus_tag="ACH3L1_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFT4078922.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          611 a          437 c          575 g          749 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggtgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggg caatttttcc ttaaggtatg aaaaaaggag
     1081 ctgaagctat ataatagcta ttaatgccac tggtatcagt gaatcatgta tataaaaaac
     1141 tgtgttaagg aggctgtcca ttagcggttt tttattcttc tggaaccgcg gcatctttgc
     1201 gtatcttttt gtcttctcac cgtctggtcg gtgttttgct gagactgcta atttcctgtc
     1261 tttgctagtg ctgtactggt actgcccaat catgattggc gggggcgggc ggagttgaga
     1321 ccgcagacac gtcgtatgca agaacgtgct gcggctggct ggtgaacttc cgatagtgcg
     1381 agtattgaat gatttccagc cgttactgat tttacgtgct aattagtgaa caaaccactc
     1441 gtcagcagac tcccaggtat ctttcagagt ttcctgaaca aaagttttag ctgaatcttt
     1501 atcggcggtg cgcgtaacag aaaggccatc gttgctggtg gcttttacga tcacctctac
     1561 atcgtcataa cgtttactga tgcgtcgggt taattcttcc tttaacgcat ccacagcacc
     1621 ggttggcatt ttagtcattt tctctttggc tatgcagatt tcaaaacgca taaaaatccc
     1681 tctgcactgt gtttttatac agtattattt ttaactgtat ggataaacag tgtcaagagt
     1741 gagggaaagt gattttttgg ggcatgcatg gggcaaaaat atttgttgtg gggcattttg
     1801 gggcatgatt gggacacttt gttacatatg aactttgccg attttcatat gacatggcaa
     1861 tccaacgctt tgattattct aaagaaaaca catgctcttg ggcgttcttt agtgattttt
     1921 aaaaatcccg tatcacgcag ttaaagtggc gggcatactc ttcaaggctg gtgatcccca
     1981 accgcaccca tttaggatgt gaccattgcg gtagaccgat gtaaatcata acgcgtgaag
     2041 gatctcttcc acgctacgca cacgggcgat gcgcgggtag atatgattaa tgctgttatt
     2101 gtgttgctcg gcgctggcgg cactacaggc gtcttcggca atcaccagat taaagccaag
     2161 ttcccaggca ttgcgggcgg tggattcaac accgatattg gtcgagatcc cacataacac
     2221 tattgtatcg atacctcggc ggcgcagttg caactccaga tccgtaccgt aaaacgcacc
     2281 ccactgacgt ttgatgattt cgatatcact gtcagttgca cctaatgcag ctggatgttg
     2341 ccaccagttt tcgggcaaca ctttagcggg gg
//
LOCUS       DAZXUL010000282         2343 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZXUL010000282 DAZXUL010000000
VERSION     DAZXUL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.284"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..124
                     /locus_tag="ACH3L1_004481"
     CDS             <1..124
                     /locus_tag="ACH3L1_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFT4078923.1"
                     /translation="CFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            complement(273..1727)
                     /gene="ospC2"
                     /locus_tag="ACH3L1_004482"
     CDS             complement(273..1727)
                     /gene="ospC2"
                     /locus_tag="ACH3L1_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFT4078924.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
     gene            2131..>2343
                     /locus_tag="ACH3L1_004483"
     CDS             2131..>2343
                     /locus_tag="ACH3L1_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFT4078925.1"
                     /translation="MSIRCPSCSATEGVVRNGKSTAGHQRNLCSHCRKTWQLQFTYTA
                     SQPGTHQKIIDMAMNGVGCRASARIMG"
BASE COUNT          713 a          440 c          406 g          784 t
ORIGIN      
        1 gtgctttggg cggctgaaag aatatcgccg catcgccacg cgttacgaca aaacggcgag
       61 aaattacctg gcgatggtga aactgggctg catccgactc ttttatcaac gcttacgtaa
      121 ttaagggaca cagcctagtc tccaggattc ccggggcgtt caccacatgc aatgtgttgt
      181 tgcaggggaa cggtctgcac catcccctat atcgatcaat ataacaaaaa tgacaacgtc
      241 agattcaggg cacaacctaa atatatttta ggtcacctcc caaatcgttt gcctgatacc
      301 gctccatttt ttaataaaaa atcaattgtt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt tgctttttga aatcttttgt ttacatcaac caatccatta
      421 ctaataaaat attctagcac tttatagttg gcactatctt cactaagtaa atactcaaca
      481 tcatacagct cacaatacat attgttgttc attttctcca tctctgcgat atcttgtttt
      541 gtaaaattaa atttagatag taaataaagt gccatatcag attttgcatt gcctagagaa
      601 taagcaagag cttgatgagc cattttttta tcagtcacct tattgttaat ttcttcataa
      661 ttggatgctt taatggcatc ctccaaagat atatctctga gtcttatctt tttgaagtta
      721 tctgtgctta gcattcttgg tatatgatac tcaagctgaa acacaaagtt tataattcta
      781 tccagactaa caggatcaat gtttttatta taacaaaact ctcggaatct ttgatcttta
      841 ctctttctta taaaatcaat aagatgcaac ccaattccaa gcctcatatc tttagtatta
      901 aatattggca catcagtttt tccctgatta ccatgcacat acctgctaac ttcatccaca
      961 acctctttaa agttatctaa gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata
     1021 gcattatcaa agtggtcggt taatgtcata tatccatatg gagagtcatg atcaatgata
     1081 tacgcattag caccaaaatc cacagtatga tgtgttttat ttagtgggag tgtttcttga
     1141 tgattggaaa tttcaacccc aaaaaaaaca aaatcgtggt tagctatttc ttcaatatcc
     1201 tcagagaatg tgtttttata tgtttgtata ttctcagact taattttatc aaaggactta
     1261 atattaagag tattatttga caggttagcg ttactttgat gtctgaagtt aaaatcagaa
     1321 ttaataataa tagagagcaa gtgacgttca tccgaagaaa gaggatgagt tttttgtatt
     1381 atttcagaaa gatagtgtct ggacacctta tcgaggtgct caagtgaccg aaatgatgac
     1441 cgtgctccag atggcgcttc aatagaaaga tttaatgatt tgaaactggt cccttgagag
     1501 aacattttac tataactttg cgctgctata gacttcctta aaaagctttt cacagtatca
     1561 gcacagtgat ctaaatgttt ttcacttatg ccagaagagg atgagtttgt tacgcatgat
     1621 atgttttttt gtaatgcttt tgtatctttg ttcggattta tttttccatc aacaagatca
     1681 atattgttct gaacattaat atgatttact gcttcaggta ttttcataaa acatccccca
     1741 ttatgtgcgt ttttatctgc agttatttag aataatatta tctaacttaa tattttcttg
     1801 tatgtattat aacgaacata ttttaatatg ttgtacgatt ttcactctga aatcccctga
     1861 gtttaatcac acttccttta ctgaaaggaa ttattgcaac agtattattt gtttttggat
     1921 atatatcttg cgccttgtta tgtagttaat gctcagaata tgatagataa aatagagacg
     1981 caaaacaggg catgctcata acatgatgat aacataaaaa attagtaaca ataaatgaaa
     2041 gaatcatggg cgctcaacaa aaagagggta atgctgccaa cttactgatt tagtgtatga
     2101 tggtgatttt aaggtgcttg cgtagcttcc atttccatca gatgtccttc ctgctccgct
     2161 actgaaggcg tggtgcgtaa cggcaaaagc actgccggac atcagcgcaa tctctgctct
     2221 cattgccgta aaacatggca actacagttc acttacaccg cctctcagcc cggtacgcac
     2281 cagaaaatca ttgatatggc catgaatggc gtcggatgtc gcgccagtgc acgcattatg
     2341 ggc
//
LOCUS       DAZXUL010000283         2326 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZXUL010000283 DAZXUL010000000
VERSION     DAZXUL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.285"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            61..282
                     /gene="kil"
                     /locus_tag="ACH3L1_004484"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="ACH3L1_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFT4078926.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            282..452
                     /locus_tag="ACH3L1_004485"
     CDS             282..452
                     /locus_tag="ACH3L1_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFT4078927.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="ACH3L1_004486"
     CDS             527..802
                     /locus_tag="ACH3L1_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078928.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..2317
                     /locus_tag="ACH3L1_004487"
                     /pseudo
     CDS             902..2317
                     /locus_tag="ACH3L1_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
BASE COUNT          720 a          531 c          529 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggtag
//
LOCUS       DAZXUL010000284         2285 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZXUL010000284 DAZXUL010000000
VERSION     DAZXUL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.286"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..110
                     /locus_tag="ACH3L1_004488"
     CDS             <1..110
                     /locus_tag="ACH3L1_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFT4078929.1"
                     /translation="PVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(199..711)
                     /locus_tag="ACH3L1_004489"
     CDS             complement(199..711)
                     /locus_tag="ACH3L1_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFT4078930.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            906..1670
                     /locus_tag="ACH3L1_004490"
     CDS             906..1670
                     /locus_tag="ACH3L1_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078931.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1621..>2285
                     /locus_tag="ACH3L1_004491"
     CDS             1621..>2285
                     /locus_tag="ACH3L1_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFT4078932.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          651 a          500 c          578 g          556 t
ORIGIN      
        1 ctccagttgc tggacgaaac gttctcggta tccgggacgg aatcatcagc gaccagacag
       61 cattgagaat gcttcaggag tacatcagga tccaatgcct tggggggtag cggtaatttt
      121 actcattatc cttcaaatca gattctgttg tcagaggaat gggggaagct ggattcgcaa
      181 gagcttttta tgggcagttt agctaaaaaa aggcaacacg aagctaaaac taccaacata
      241 aagtgctgac cagctagcag ctgaaatcag actcactgta tacactttca ctggatgaag
      301 ttgcaacatt cctgccacca aaggagcaat gtaacgcagc acggcgataa agcgcgaagt
      361 gaaaagtatt gacacagagt tattttgcaa ctgaaggcga acccgttcta ttgtattgga
      421 acggctcgat attatacatg caagttgagg gatatgccca attattatac caagatggta
      481 attaacgata gtaccgcacc atgcgccact catgaccgtg attccagctt cccatggtga
      541 aagagtcgtc ttgctgactg taattacagt ggccatcata attgatgctg gtggcaatat
      601 cgccgaaatg agtaaagtgg attttgtgaa cgctattata aaaagaagcc cccataggcg
      661 atatggatga agtacaaaat agttcatcaa agcatttatc cactccatca atgtctttcc
      721 ttctgtgaat ttttctttgc tatttaaccg tgatacgact taatttaggc ttaaacacct
      781 tacatttaca aggtactcct gtggggggcc ctgccacgga gcgtcgcagg agtgagtgat
      841 gaggaagaat taaagcaacc tattgtgaca ggcagcgata acagtaaatc acaggaagca
      901 taatcatggc aaaaccggac tgggaggcca tcgaatcggc ataccgggcc ggagtcctta
      961 gcctccgtga tataggcgag aaatacggcg ttacagaagg ggctatcagg aagagggcca
     1021 aaaagcttga ttgggcacgc agtggcggta cgcaggtttg caaaaatggt acgcaaaaaa
     1081 ggaaagtgcg taccagcaga aagcctgcca ttactggcct tacacaaaaa agtacgcaac
     1141 taaaaacaga atctacaccg gatacgaaac cgatacgcgg aatgcgtacc gatcccccga
     1201 ctaacccatt ccaacccggt aaccagcagg cattaaaaca cggtggttat gcccgtcgcc
     1261 ttctgctcaa agatgaggtg atagaggacg ctaaagcgtt gacgctcgag gacgaattat
     1321 ttcgccttcg tgctaacaac cttgtcgctg cagagaatat tggtcggtgg ttggtgtcgc
     1381 tggaagatgc taatggggac caggaaagga agatgctgat tgaaaatatc agcgccgccg
     1441 agaaagcaat gatgcgcaat acagttcgta ttgagtccat cgttggcacg cttgcgacgg
     1501 taggaaaaat atttgctgat acagcctacc gcaaggccgc cactgataag gtgtctctgg
     1561 aggctgatcg tcttcgccgt gatgcaggta ttgatgatgg caatggagag cgtgacctca
     1621 atgacttcta ctctgacatc caaaccgacg ctgaatccgg ctttacgtag tttctggact
     1681 atgcgggcac gtaacaaagt gctttatggt ggtcggtcat cgtcaaaatc atgggatgcc
     1741 gctggcattg ccatatttct gtcgaataaa tacaccctgc gtttttgttg tgcccgtcag
     1801 atccagaata aaatcgaaga gtcggtgtat accctgctca aaattcagat agacaggttt
     1861 ggtctgcggc accgtttccg tattctgaac aacaaaatca ttaaccgggt tactggctcg
     1921 gaatttgttt tttatggatt atggcgcaac atcgaagaaa ttaagtcact ggaggggatc
     1981 gatatgttgt ggttggaaga agcccacgca ctgacggaat accagtggaa aattctggag
     2041 ccaacgatcc gtaaagaggg ttcggaatgc tggttcatat tcaaccccgg acttgttact
     2101 gatttcgtct ggcgcaactt cgttgttgat ccgcccgaag gcactctcat ccgcaaaatt
     2161 aactatgacg aaaatccgtt tctgtctgac accatgctta aggttatcga cgcggcgcga
     2221 cgccgtgatc cggatggttt taaacatgtg tatgagggcg ttccggagtc tgatgatgat
     2281 gcggc
//
LOCUS       DAZXUL010000285         2281 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZXUL010000285 DAZXUL010000000
VERSION     DAZXUL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.287"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(564..2168)
                     /locus_tag="ACH3L1_004492"
     CDS             complement(564..2168)
                     /locus_tag="ACH3L1_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4078933.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          591 a          553 c          505 g          632 t
ORIGIN      
        1 agttaacttt aagttaagcc cctgtaggga ctagagacca taattaagat gattgtgtct
       61 tattgctatt cacttatcga ctgacccaga gcacacagga agcattcact ttaagtagaa
      121 cgcttggtgt ctgtaattgg tcatcccgcc cctgaaagga tgcgataagt tacggttagt
      181 tgtcctagaa gagaaaactt tttgtgtccc ttctccctat agtgagtcgt attaattatc
      241 cttgacaagt agagaaaacc atgagtcaaa aacatgacca cacaggtctg tagtaagtgt
      301 catcctagaa gagaaaactt tttgtgtccc ttctccctat agtgagtcgt attaattacc
      361 ccttgacaag cagagaaaac tatgagtcaa aaacatgacc gcacaggtct gtagtaagtg
      421 tcatcctaga agagaaaact ttttgtgtcc cttctcccta tagtgagtcg tattaattat
      481 cccttgacaa gtagagaaaa ctatgagtca aaaacatgac cacacaggtc tgtagtaagt
      541 gtcatcctag actatggtct cctctaggtc tccttataaa catcagccat cacattgacc
      601 agcctatcaa cctcgacctc tttagcataa cggtcgtacg taatagaccc tgaactatgc
      661 cccatgatcg cctgtgcata cgccaactcg accccatgtt gttttaactg ggccgcaact
      721 gtatgtctaa acgagtgaaa cgctaagccg ctacggtcta cgaggacttt aggcaacaac
      781 gtccgattaa accatttact cacctgctca ccgtaaccgt tcttcatgag acgcagacca
      841 tcgaacacca tagcgtcatc accgtcagcg cctctacggg tctctacaag cgccataaag
      901 tcagccaata taaaaccata ggccccatcg accagaggca cacagcggtc actatgagcg
      961 ttcttgatgc tcttaccggg tagactacta tcgtcttcat tgatatgaat ataaacggtt
     1021 cccgcctcag tggtcctgac gtctttgacc tgtagttggg ctatttcatt gagtctcgcc
     1081 ccagtgatag cagccaaggc ggtaacataa taatgatacg gcttaccaga ggtcttctga
     1141 gagtatgcct tagctgcgac cagtagttgc cccacctgct ccgtagagaa ggcgttacgg
     1201 gcccctgagg ctttacgctg agggacttta agctccagac cctcagtcat gttcttttta
     1261 atcaagtcat tgcgcacagc ccacctaaag acagcagcca tcttaataag atacttgttg
     1321 ttgatggtaa cgacatccag acaatctgtc ttatcttcac ggctcaacag gtcagcaagt
     1381 gggacatcct tgaagcgctg cttacggttc ctaggtaact gctggagcac atcacggacc
     1441 ctaagcatgt cagccctgtt agcgtctgca ttgagaccca gatggtcgaa tatttcgatt
     1501 aacgcagcgt gtgacgcctt gttttccctt aaggttgcag gcttccagtt ctgatagttc
     1561 tccgcttcgt attgttcaaa gaggctcgct atggtaatgc taggcgtaac ttctggtttt
     1621 acttctggag cattaacaga tagggacaca gagtctgctt ggttatcttg cctatcaata
     1681 tcattaaaaa gatcgacgta atctatcaga ggctgactgt ctcccgcttc gatacgcctc
     1741 atacaggctt ttagcacctt cagggcctca ttgatgtaac gatgctggtc aatgcttagc
     1801 ggctcactag ccagagcatc ggtaaggctc tcaccaagtt ctccgtattg gtcccggtag
     1861 atatccctca tgtcaggctc aaagagatct gaacggccca tagacagttc acactcagcg
     1921 atgtccttaa ggtgttccct catttcctca taggtagcgt tgggtctgtc tgcgtgtatt
     1981 gccttgagtg ctgcctttat gtgccttgag taagccatag cggtgcgtct atccttggtc
     2041 cgtaatgata ttgaggtcat tctatcattg tttgattgtc tgaccctaag acggaagtag
     2101 tagacgccgt tacgctggta gagatagtag actgctggaa gtttgtgact actggatagc
     2161 tgaatcatat gtctgcatgc cctgagaggg ttacacagtg ggttacagta agtaactctg
     2221 caaggaggct taaagccgta tcccttagga ttcagtaggt tggagtactt taagaaaggt
     2281 c
//
LOCUS       DAZXUL010000286         2250 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZXUL010000286 DAZXUL010000000
VERSION     DAZXUL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.288"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..242)
                     /locus_tag="ACH3L1_004493"
     CDS             complement(<1..242)
                     /locus_tag="ACH3L1_004493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078934.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHARPELRA"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="ACH3L1_004494"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="ACH3L1_004494"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFT4078935.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          553 c          666 g          457 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac
//
LOCUS       DAZXUL010000287         2248 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZXUL010000287 DAZXUL010000000
VERSION     DAZXUL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.289"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..134)
                     /locus_tag="ACH3L1_004495"
     CDS             complement(<1..134)
                     /locus_tag="ACH3L1_004495"
                     /inference="COORDINATES: protein motif:HMM:NF024418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFT4078936.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     "
     gene            complement(495..1499)
                     /locus_tag="ACH3L1_004496"
                     /pseudo
     CDS             complement(495..1499)
                     /locus_tag="ACH3L1_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            1484..1861
                     /locus_tag="ACH3L1_004497"
                     /pseudo
     CDS             1484..1861
                     /locus_tag="ACH3L1_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1900..>2248)
                     /locus_tag="ACH3L1_004498"
     CDS             complement(1900..>2248)
                     /locus_tag="ACH3L1_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4078937.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT          539 a          518 c          596 g          595 t
ORIGIN      
        1 tatattgagg ataaaataat accgaggata actttctttc ttgatgtacc gacactaact
       61 ttccgggtca gttcagaaac agcaactaat ccacctgtaa tatgactata tttcagtgaa
      121 taaattttat tcatacgtga actccgatat ttctgagttg tggttatggg atagcattta
      181 attacaacgg ggaactatca gtcatggtac gtaactgata agggtataat gataatcact
      241 gtcagttata tttgtatgac agatgtaaca aaatgaaaaa aaagataagc acaagtgatt
      301 gattggttag atattgatca cttggtgttt gtattttgaa ttttgcgatc aaaatttgat
      361 ctttgttgtt tccggtcaat cctttacagg ttttctgtat tatccggggg agttaagtct
      421 gtcgatgtcc gacagacttt tgatgatgga aacaacatgc agcgataatg tgcatgctgt
      481 tttgcaggtg gaactcagaa ggtgaactgg aactgtgcga ccgcaccgta agtgttgtcc
      541 gtgccgatca ggcctgcaac atcaaggtga accacatcaa acggtgagat ccccaccccg
      601 gccgtgaatg cactgccgtc gttaccggcc agattctgac ggtaaccggc gcgcagctgt
      661 gcccatttcc aggcattaaa ttctgtgcca atggcggcaa actgacggtt gctgtcggag
      721 gtgaaaccgc tggccggggt cagatccaca tcaaatgcag tggtgaacat cgcgttgtgc
      781 caggagacac cggccgtcac ctgcgggcgg atcctgaagg tttccgtgat accgttcact
      841 tcttttgtct caatactacg ggagatcagg ttctgtgcga ccagtcccag cgtccagttg
      901 tcatccaggt ccgtactggc accgatgtcg gcgttgatgc catttttcgt gttgtggtag
      961 cggtcgcctt tgaaggcgct gctgtcataa tttctgacca gcacgttgta gttgaacagg
     1021 tcgacgcgct ggaatttggg ggggaagccc agagaccatt tctgcccggc cgtttccagt
     1081 tccgtagcca gagcaatacc cacatcagtg atcagtgccg tgcgggcaca ggcctgtgat
     1141 gtcaggcggc ttttgtccac ctcctgcccg gtgctgtttg ccacctcttc caggtaagtg
     1201 aggtcagcat cagagacttt accgtcgaca ctgacggtgc cgtgtgattt aaccatcagt
     1261 gcggcagaga ggcgatcacc gggaagggcc gccacagcag aaacgcccag ctgtgcggcg
     1321 gcatgtgtgt tacggaagtc ccggaggtgt tctttcaggc gtgcggcagc ctcaggaaca
     1381 ccgtggttac tttcgatggc gcggtcgaag gccttccact catcactgat ccggtcggcg
     1441 ttgtcggtga tattatccgg attggagagc tgcgcgccga cggacggaag caggatgctg
     1501 ggctcatgcg cgacgcaaaa tccacgatgt atatatcagt actaaaagcg cgacggcgga
     1561 agaagctctg aaactaatcg gtgaactgta cgccatagag cacgatatac gcgggttacc
     1621 ggtgtctgaa cgcctggcgg tcaggcaaat gcagagtaga ccgctcctga cttccctgta
     1681 taagctgatg caggagaaag aacacacgtt atcgaagaaa tgtcgtctgg gagatgcgtt
     1741 ccggtatatc aggaagcact gggctgcgtt gtgcaacttc tgtgatgatg gcctggctga
     1801 ggtagataat aatgcagcag aaagagcgct tcgtgcagtc tgtctcggga agaaaacttt
     1861 ttcgaggatc actcattccg tgtctgactg cttcagggtt taattttgct tctgctgact
     1921 gaatgatgat tgggcagcaa gtgcaatatc ccacataaat ccagtcaact cacgagccac
     1981 cgccgtaatg gctacttgtg attttttccc ttttgctgtc agagttcggt aacgagaaca
     2041 caatcgttgt tgagctttcc atgcaatatc tttggaatat tgtgttacag agtccggtcg
     2101 gtaatatata agccatgctc caacttttgc aggtgtacga taagaccagg cggcttcgta
     2161 aagcagacgt ctcagttcgc tgtttccaac ttttgttatt cctctgggac gaatactgtt
     2221 tccactggaa tattcacctg gcaccagt
//
LOCUS       DAZXUL010000288         2225 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZXUL010000288 DAZXUL010000000
VERSION     DAZXUL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.290"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            249..1286
                     /gene="curA"
                     /locus_tag="ACH3L1_004499"
     CDS             249..1286
                     /gene="curA"
                     /locus_tag="ACH3L1_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFT4078938.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1484..2149
                     /gene="mcbR"
                     /locus_tag="ACH3L1_004500"
     CDS             1484..2149
                     /gene="mcbR"
                     /locus_tag="ACH3L1_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFT4078939.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQSQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          612 a          471 c          598 g          544 t
ORIGIN      
        1 cgttcgagca ttataacgaa tggcatccgc atagtgcgct gggttatcgc tcgccacggg
       61 aatatctgcg gcagcgggct tgtaatgggt taagtgataa cagatgtctg gaaatatagg
      121 ggcaaatcca acggattcct cgatgaaatt attgcgtgtt gcagagggga tgtgacggct
      181 gcaaacaaaa ccaatcccgt gagcctgcta ctattgatgg attcatgcca aaagcacagg
      241 agagcattat ggggcaacaa aagcagcgta atcgacgttg ggttctggcc tcgcgtccac
      301 atggcgcacc ggttccggag aatttccgtc ttgaagaaga tgatgtcgcc acaccgggtg
      361 aaggacaggt gttactgcgc acagtttatt tgtccctgga cccgtatatg cgtggacgta
      421 tgagcgatga gccatcttat tcaccgccgg ttgatattgg cggcgtgatg gtcggcggta
      481 cggtgagccg tgtcgtggag tcgaatcatc ctgattatca gcctggcgac tgggtgctgg
      541 gctacagtgg atggcaggac tatgacatat ccagtggtga tgacctggtg aaacttggcg
      601 atcatccgca aaatccatcg tggtcgctgg gtgtgctggg gatgccaggc tttaccgctt
      661 atatggggct gctggatatc ggtcagccta aagagggcga aacgttggtg gtagctgcgg
      721 cgacagaacc agtgggggcg acggtggggc aaatcggcaa acttaaaggt tgcagagtgg
      781 tgggggtagc cggtggcgcg gaaaaatgcc gccatgctac cgaggtgtta ggcttcgatg
      841 tttgccttga tcaccacgcg gacgattttg ccgaacaact ggcgaaagcg tgcccaaaag
      901 gtattgatat ctattatgaa aacgtgggcg gtaaggtatt tgatgcagtg ctaccattac
      961 ttaatacatc tgcgcgcatt cccgtttgtg gcttagtgag cagctataac gctacagagc
     1021 taccacccgg tccggatcgt ttacccctgt tgatggcaac ggtgctgaaa aaacgtattc
     1081 gcttgcaagg gtttattatc gctcaggatt atggtcaccg catccatgag tttcagaagg
     1141 agatggggca atgggtgaaa gaggataaaa tccactaccg cgaagatatt actgatggtt
     1201 tagagaatgc gccacagacg tttatcggcc tgctgaaggg taaaaacttc ggcaaagtgg
     1261 tgatccgcgt cgcgggtgat gattaaagtt tcaacggcgg cgtaagccgc cataaaaatc
     1321 caggcaaaaa gcttattttc agctttaatt aactaaccac gtcattgttc tgctgctaat
     1381 aacaacaaag gtgggtggtt tatgattatt cactttaata cactaggtga actccttctg
     1441 ccatgcaggc agggttggac agaaaacacg cgaaggaatt aaaatgcctg gaacgggaaa
     1501 aatgaaacat gtcagtttga ctctgcaggt tgagaacgac ctgaaacatc agcttagtat
     1561 tggcgcatta aaacctggcg cacgcctgat tactaaaaat ctggcggagc aattaggtat
     1621 gagtattaca cctgtgcgtg aagcattatt acgtctggtt tcggtgaatg cgctttctgt
     1681 cgcacctgca caagcattta cagtaccgga agtgaggcaa agtcagttag acgaaatcaa
     1741 tcggatccgc tacgaactgg aattaatggc agttgccctg gctgttgaaa acctctcctc
     1801 gcaagacata gcagaacttc aggaattgct cgggaaatta cagcagtcgc aagaaaaggg
     1861 cgacatggaa caaatcatta atgtgaacag gctatttcgc ttagcgattt atcatcgctc
     1921 aaatatgccc atcctgtgtg agatgattga gcaactgtgg atcaggatgg gacctgcttt
     1981 acattatctt tatgaagcga ttaacccagc ggaattacgg gagcgcatag aaaactatca
     2041 tctattactc gctgcgttaa aagcaaaaga caaagaggga tgcaaacatt gtcttgctga
     2101 aattatgcaa caaaatattg ctattttata ccagcaatac aatcgttaaa ttaaatgccc
     2161 ggcaaatacc gggcatcaag taattactca aatcgccacg caatattcat gccgacacca
     2221 taatt
//
LOCUS       DAZXUL010000289         2218 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZXUL010000289 DAZXUL010000000
VERSION     DAZXUL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.291"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            137..>2218
                     /locus_tag="ACH3L1_004501"
     CDS             137..>2218
                     /locus_tag="ACH3L1_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HFT4078940.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIY"
BASE COUNT          779 a          429 c          397 g          613 t
ORIGIN      
        1 cagatttaaa cattatcctt ttccatatag atattggata aaaataggac atctgtttgc
       61 aattactttc acaacaatta aacattttta tgttttcgta tacatcatat tactctacca
      121 ttagaggaac gttattatgt tttctataaa accaggacct ggaaatttac ctatcgacaa
      181 ccccacattg ttatcatgga acattactga tggggatcta aattccaaat ttaatacatt
      241 agaatatcta aactgtataa caaatattat taatgcttgt ggagtttatc ctcaggattt
      301 aaaagacaga gaaattatat caacttttca cgcagaaaaa gtcattaatg atctgttaaa
      361 aaacgattat aaaatttccc tttctccaga tacaacttac cgagagctga ataaagctgc
      421 acagagtagc attacagcgc cagacaggat aggagaaggg aaaacatggg tttatcaacg
      481 agatacaatg gttgaaagag gtgataacag tggtgttcat cagtatggtc cagctgaaca
      541 tttcactcac attatatctg acaaaccgtc cccaaaagat aagtatgttg catatgctat
      601 taacattcct gactatgagc tagcagccga tgtatataat attaacgtga cgtcaccttc
      661 cggacagcaa gaaacattta aaatactaat caatccagaa catctacggc aaacacttga
      721 gcgtaaatct cttactgctg ttcagaaatc acaatgtgaa atcatcaccc ccaaaaaacc
      781 tggcgaagcg attcttcatg cttttaatgc cacctaccag caaatcaggg aaaatatgtc
      841 tgagtttgca cgttcccatt atgggtatat acaaatccct ccagtgacaa ctttccgcgc
      901 cgacggacca gaaactcccg aagaagaaaa aggttactgg tttcacgctt atcaacccga
      961 agatctttgt accatccata atccaatggg ggatttgcag gattttatcg cattggttaa
     1021 agatgctaaa aaatttggta tcgatatcat tcctgattat acctttaact ttatgggaat
     1081 cgggggaagt ggtaaaaatg acctggatta tccctctgct gatatacgag cgaagatcag
     1141 taaagatata gaaagtggta tccctggcta ttggcaaggt caggttttga ttccattcat
     1201 taaagatcca gtaacaaaag aacgtaaaca aatccatcca gaagatatac atctcactgc
     1261 aaaagacttc gaagcaagta aagataacat ctctaaggat gaatggaaaa acctccatgc
     1321 attaaaagaa aagagtttaa atggaatgcc taaaacaacc cccaaaagtg accaggttat
     1381 tatgttgcaa aatcaatacg ttcgtgaaat gcgaaaatat ggcgtacgag gtttacgtta
     1441 tgatgcagca aaacactcaa aacatgaaca aatagaaaga tcaataaccc caccgcttaa
     1501 aaattataat gagcgattac acaatactaa cttatttaac ccaaaatatc atgaaaaaac
     1561 cgttatgaat tacatggaat atctggtaac ttgtcagttg aatgaagaac aaatgtcatc
     1621 gctgctttat gaaagagatg atttaagcgc cattgatttt tcattgctca tgaagacgat
     1681 aaaagccttt tcattcgggg gagatctcct aacccttgca tcaaaaccgg gttccacaat
     1741 ctcaagcatc ccatcagaaa gacggatatt gattaacatt aaccacgatt ttcctaacaa
     1801 tggcaatctt ttcaatgact ttctatttaa ccatcaacaa gatgaacaat tagcaatggc
     1861 atatatggcc gctctcccgt tcagcaggcc tttagtttac tgggatggcc aggtattaaa
     1921 atcaacgact gaaattaaaa attatgatgg gcccacgcgt gtcggtggtg aggcgtggct
     1981 taataaaggt tgctctacct atcagcagct ctacaatgaa ttccacgcat tatatataga
     2041 taaagcagga atatggagcg catttgaggg tgtatttgca actaaaaacg ttctggcctt
     2101 tagtcgtggg gattctgtga acattaatca ctctcctcat gatggactag ttataataaa
     2161 taaaggaaac gaagaagttg aaggtacctg gcctaacaaa ttgcaacctg gaatatat
//
LOCUS       DAZXUL010000290         2218 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZXUL010000290 DAZXUL010000000
VERSION     DAZXUL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.292"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACH3L1_004502"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACH3L1_004502"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFT4078941.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACH3L1_004503"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACH3L1_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFT4078942.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACH3L1_004504"
     CDS             complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACH3L1_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFT4078943.1"
                     /translation="MQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          611 c          555 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DAZXUL010000291         2206 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZXUL010000291 DAZXUL010000000
VERSION     DAZXUL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.293"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..334)
                     /locus_tag="ACH3L1_004505"
     CDS             complement(<1..334)
                     /locus_tag="ACH3L1_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078944.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            496..552
                     /gene="ysgD"
                     /locus_tag="ACH3L1_004506"
     CDS             496..552
                     /gene="ysgD"
                     /locus_tag="ACH3L1_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFT4078945.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            704..1654
                     /gene="corA"
                     /locus_tag="ACH3L1_004507"
     CDS             704..1654
                     /gene="corA"
                     /locus_tag="ACH3L1_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFT4078946.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1695..2075)
                     /locus_tag="ACH3L1_004508"
     CDS             complement(1695..2075)
                     /locus_tag="ACH3L1_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFT4078947.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2089..>2206)
                     /gene="yigG"
                     /locus_tag="ACH3L1_004509"
     CDS             complement(2089..>2206)
                     /gene="yigG"
                     /locus_tag="ACH3L1_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFT4078948.1"
                     /translation="TMIAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
BASE COUNT          570 a          526 c          515 g          595 t
ORIGIN      
        1 ccttctgctg accgttttcg atgtacaaac cgagcggcgc atagctttca tcatagatgc
       61 cgccgttcat cgccatctgc acctgaccct gactattaat atccgccagc agagcatgta
      121 acgttcccca cgcttcgcca ttggcttttt gccagtacat tttcacccgc tctgtttgag
      181 gattaacggt atacgcctgt acggtcagcg tcggatctga gagtgcgcaa tcatcagcgg
      241 caacagcaaa caagggaagt aaggtgaggg tgagaaaaat ccgtttgaga ttcaaggtga
      301 tcattccttt accaatgagt agccgatgcg ccattatagg tcctagatgt gggatttttt
      361 tatcctgtta gcgaccttga cgagtaccaa aaagcgcgaa gttcaactat tgttctgtgg
      421 tgttctgttg cgtgttgacg gcaaaatttt gctggcgtaa catgcgcgca cgatcactct
      481 aagaggacat tcgccttgga cacacccagt agatactggc tcactatcct gtcatccagg
      541 atcaactcct aaggctatcc ctttttgctg atagccttag cggttgtcag cgacctcaat
      601 ttttcccgtc gcgctgagtc aggctgttta atggtctgaa acccaatttg tttctgtgtg
      661 cccaccgaac tgtccgatat tttaagcatt gggagtcccg gtcatgctga gcgcatttca
      721 actggaaaat aaccgactga cccggctgga agtcgaagag tcacaacccc ttgtaaatgc
      781 agtatggatt gatcttgtcg aaccggacga cgacgagcga ctgcgcgtac aatctgaact
      841 tggccagagc ctggcaaccc gcccggaact ggaagacatc gaagcatcgg cacgtttctt
      901 tgaagacgac gacggcctgc atattcactc cttcttcttc tttgaagatg cggaagatca
      961 cgccggtaac tccactgtgg catttaccat ccgtgatggt cgtctgttta ctctgcgtga
     1021 gcgtgaactg cccgcttttc gtctgtatcg tatgcgtgcc cgtagccagt cgatggtaga
     1081 cggtaacgcc tacgagttgc tgctggatct gttcgaaacc aaaatcgaac agttggcaga
     1141 tgaaattgaa aatatctata gcgacctgga gcagttgagc cgggtgatta tggaagggca
     1201 tcagggcgat gagtacgacg aggcgctctc cacgctggcg gaactggaag atatcggctg
     1261 gaaagttcgc ctgtgtctga tggataccca gcgcgctctc aacttcctgg tgcgtaaagc
     1321 gcgtttaccg ggtgggcaac tggagcaggc gcgtgaaatc ctgcgcgata tcgaatccct
     1381 gctgccgcat aacgaatccc tgttccagaa ggtgaacttc ctgatgcagg cggcaatggg
     1441 ttttatcaac atcgagcaga accgcatcat caaaattttc tcggtggtat ccgtcgtgtt
     1501 cctgccgcca acgctggttg cttccagcta cgggatgaac tttgagttta tgccagaact
     1561 gaagtggagt ttcggctacc ctggcgcgat tatctttatg atcctcgcgg gcctggcacc
     1621 gtatctgtac tttaagcgga agaactggtt gtaaaaagaa ggcggtggct tagcctggct
     1681 aagccacctg ttattcaaag gctccaggta tttaaccctt ttacctcttt ctcataaaac
     1741 catttgttcg tgttaacagc aacataggct gctacggcaa ttcccagaat gttaacgcca
     1801 attaatgcac caacaaatag agatagaatg ccaagtaata gaactataat tgcttttttc
     1861 cataagccca gaacaaataa atagatccag gaacagaaga aagcaatgaa attcatttga
     1921 atagttaatc gttgtcttac ttttaatgct ttaaatgctg ctttatattc tggtgttgct
     1981 ccccagaatc caggaaagcc atgttgatca taaaaattaa atcggtattt ccatttttca
     2041 cttaatgaat catcgttcat atattcctta ctcataaata ctccataact attgttttga
     2101 tgaatcagta ggtgcaagca ttagcatact gaaagtggaa aaataacaaa tcaaaaaaat
     2161 catcgaacca ttgcctgaac aggcaaaatc ttcggctatc attgtg
//
LOCUS       DAZXUL010000292         2203 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZXUL010000292 DAZXUL010000000
VERSION     DAZXUL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.294"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..132
                     /locus_tag="ACH3L1_004510"
     CDS             <1..132
                     /locus_tag="ACH3L1_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFT4078949.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
     gene            319..556
                     /locus_tag="ACH3L1_004511"
                     /pseudo
     CDS             319..556
                     /locus_tag="ACH3L1_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            670..928
                     /locus_tag="ACH3L1_004512"
                     /pseudo
     CDS             670..928
                     /locus_tag="ACH3L1_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            977..1555
                     /locus_tag="ACH3L1_004513"
     CDS             977..1555
                     /locus_tag="ACH3L1_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078950.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1664..1922)
                     /locus_tag="ACH3L1_004514"
                     /pseudo
     CDS             complement(1664..1922)
                     /locus_tag="ACH3L1_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1906..2203
                     /locus_tag="ACH3L1_004515"
                     /pseudo
     CDS             1906..2203
                     /locus_tag="ACH3L1_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          691 a          392 c          547 g          573 t
ORIGIN      
        1 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
       61 aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg
      121 tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      181 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      241 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      301 ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat actgacgtca
      361 aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc aacagcgatc
      421 ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg
      481 taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtggaaaa
      541 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
      601 cggtcaaaat gcttctgccg tgaaggaagg agaatgccat ctcatcactt atatctggcg
      661 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      721 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
      781 ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca
      841 tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga
      901 aaatgatagc tggtttctga aaatatagag gaaaatgtga tatatatatc ctgttaatat
      961 ttaaggagaa tataaaatga aagtctcgtt taagtcttta ggctatattt ttcatgatat
     1021 atataataaa aaacacacta tcgatgagtt caacgatgtg gtaagaaaag cagttttatc
     1081 aggtaaaatt aacgagctga atgcatgtca taaagtggca atttttcttg ccgaaaaaga
     1141 caatgagata acaaagaaag acaaagccaa gataatagat acattaactg aaaactattc
     1201 cattgaattt caacaattaa tgaatattag tgagcgcacg ttaaattcat ctttatatat
     1261 tactcctggt gaaagtggtt ttgtcagttt tgttaatcgt gaaggaaaaa tatgtcatac
     1321 tgcatatgta aaatcatcag ataatagcat gacatattac catgcaaatg gtagcagcat
     1381 tgacaaatat attacggata tgtgcggcct tatttgtatg aggcatattg agtctactgg
     1441 cattattttt tatatgcttg atgaaaaagt tttatctgcc attgctgaat ttatgaatga
     1501 aaaaggatgg cgggctgcgt tttgttcagc aaagaactta tataaatgtg tttaatttta
     1561 gaaaaactat ttggtaaaat ccaacaagag tcgagaggtt cgcgttgctt cagcagggcc
     1621 tcagcctgtt caaccagact cagttgcagg tcgtgacgca gatttagtgg tggtggttga
     1681 ttgtcagata ccagccgatg atcttgtagt gcatatcatc agactttgag aagcatatgg
     1741 ttttgcgggt aagccgctta agatgtgtcc gcaggttcag attatgtcgc tctatccgct
     1801 gagtgcattt cttgcttata acatgactgg ttgaggacaa taaagttcgg tataccggct
     1861 caggcatcgg tcatgtagaa ggcaatgcta aactggctca gcaaactaag agcagcgcgc
     1921 atccacttac ccaatgcggt tgagaaaata gccttcgatc gctttcatgt ggcgaaacaa
     1981 ccgggtgagg tggtggataa aacccgccag aatgaacatc cgcatcttcc tgttgaaagt
     2041 cgtcgtcagg caaagggaac tcgcttcctg tggcagcaca gcgataaatg gatgaccgaa
     2101 tccaggcagg aaaagctgat atggctgcgt gcgcagatga agctgacgag cctgtgctgg
     2161 gcgctgaaag aactggcaaa ggatatctgg agcagaccat gga
//
LOCUS       DAZXUL010000293         2198 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZXUL010000293 DAZXUL010000000
VERSION     DAZXUL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.295"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="ACH3L1_004516"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="ACH3L1_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /gene="yniD"
                     /locus_tag="ACH3L1_004517"
     CDS             1128..1235
                     /gene="yniD"
                     /locus_tag="ACH3L1_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFT4078951.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="ACH3L1_004518"
     CDS             complement(1288..2046)
                     /locus_tag="ACH3L1_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFT4078952.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          661 a          485 c          457 g          595 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattagcca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcat
//
LOCUS       DAZXUL010000294         2187 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZXUL010000294 DAZXUL010000000
VERSION     DAZXUL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.296"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..206
                     /locus_tag="ACH3L1_004519"
     CDS             <1..206
                     /locus_tag="ACH3L1_004519"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078953.1"
                     /translation="ARAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVE
                     NVVDVYVTHNPKKTEQKAGVSIH"
     gene            230..697
                     /locus_tag="ACH3L1_004520"
     CDS             230..697
                     /locus_tag="ACH3L1_004520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078954.1"
                     /translation="MYGGSPADIAAAIWRKAPPGIDMNGNTTFTVADKEYDPPYPEYV
                     ISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGR
                     YYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            699..1304
                     /locus_tag="ACH3L1_004521"
     CDS             699..1304
                     /locus_tag="ACH3L1_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFT4078955.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1291..>2187
                     /locus_tag="ACH3L1_004522"
     CDS             1291..>2187
                     /locus_tag="ACH3L1_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFT4078956.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVF"
BASE COUNT          532 a          509 c          610 g          536 t
ORIGIN      
        1 acgcccgtgc cggtgtgccg ggcagcgatg aggaaacccg tgcggacttt gaaaaccgcc
       61 gacgtaattc agttgcccgt aatgcccgga atattctgga agccatccgg ggtgaaatac
      121 tctccacggt agaaaacgtg gtggatgttt acgtcaccca taatccgaaa aaaacagaac
      181 aaaaagccgg agtcagtatc cattaacacc cggttcgctt tatgttggcg tgtacggcgg
      241 cagcccggca gatatcgcgg cggccatctg gcgtaaggct ccgccgggta ttgatatgaa
      301 tggcaacaca acgttcactg ttgcagataa ggagtacgat ccgccgtatc ctgaatacgt
      361 gatcagctgg cagacactca aacccgtcag tctgcatgtc agtgtgacgc tgaaaaaaag
      421 tgactacctg ccttcagata ttacccgaca ggtacagcaa tccgtgctgg acgcatttaa
      481 tggtacagat ggtggtctgc gggcaagagt tgcctctgtt gtctctgccg ggcgctacta
      541 tgccggcatt tacaaaaccg atccggaaca tattgatatt ctgggcctta ctgtgagccg
      601 tgatggttcg tcatggacaa cggctgtcac tttcgggata gatgagattc cggttctgga
      661 tgtgtcggat atcagtgtga aactccagga ggcataacgt gcagaatgtg gctgccactg
      721 tgcttgcgca gtatgctgcc agcccccgac tcaatgccct cattaacagc tttaacgcag
      781 cgctttcccc cgacagtttt atcagtgatt tttatggtct tatctggaac atcgataccg
      841 cagaaaagta tggtcttgat gtctggggaa agattgtggg tgtcagtcgc tggctgacgg
      901 taaaggacga ttttaattac ctgggcttca gcgagtccag gatggacacc ccggtaatgg
      961 atgatccctg tccgtttaat caggcaccgt tttacaacgg aaaatcggat acccggactg
     1021 ttgacctgtc tgatgctgta taccggcggc tgatactgat gaaagccatg tcgaacatta
     1081 ctgactgttc cgttccggat attaacctga tgctgagatt tatgttcgga aaaaaacgcc
     1141 gggcttatgt tctgaataat ggcgggctga ggatgagtta cgtctttgag tccgcgctct
     1201 cgttggcaga actggcgatt atccagtcgt cgggtgcact gccatccccg ccgggtgttt
     1261 atgtttcagt agttttaaag gagtcccgta atgaaggcca gtgataaacc ccgccagctg
     1321 gcggtcccct ttgcgagtac cggagataaa aaccgtatcc cggacaaggc gacacagcag
     1381 accagagaga gcggtaatgc tgcgtatgat tcaggttttc ctccggtgac catgacagcg
     1441 gtctcagcgg gaggtatacc gccacacggc aaggatttta acggtctgat gtacgatatt
     1501 accgcagcaa tacggttcgc ccaggctggc ggtttgtaca cgtataatgc cggttttgcg
     1561 ggggccattg gtggatatgc aaaaggagcc attctcgccg gagtcgcaac aacagcggtc
     1621 tggctgaata ccacggacga taacctgacc gatcctgaag gctccgacag tgcgggctgg
     1681 gtaaatcttc ttgaggatcc gaaaaggata ttcctgcggc agaagaacaa tctgtcagac
     1741 cttcagaata aagggacggc acgggataat cttcaggttt acagtaaaga gcagtcagat
     1801 caacgctatg ttcatcggga aggcgataaa ataaccggag agctgaaaat ccgtggtgtt
     1861 aatgcgctga ggattttcaa cgaagctttt ggcctgattt ttcgtcgttc ggaagagtgc
     1921 ctgcacctta ttcccaccag tgaaggtcag ggggaaaatg gcgatattgg tcccctgcgc
     1981 ccgttcacca ttaatttgcg gacgggtgaa atatccatgt cgcataaagt gtctgttggc
     2041 ggtggttcgc aggtcaatgg tgcgctgggt atcggcgttc agaacgccct gggggggaat
     2101 tcaattgttc ttggtgataa tgacaccgga tttaaacaga atggagacgg tattctggat
     2161 gtttatgcta atagtcagcg tgtattc
//
LOCUS       DAZXUL010000295         2187 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZXUL010000295 DAZXUL010000000
VERSION     DAZXUL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.297"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(220..384)
                     /locus_tag="ACH3L1_004523"
     CDS             complement(220..384)
                     /locus_tag="ACH3L1_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078957.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="ACH3L1_004524"
     CDS             1286..1570
                     /locus_tag="ACH3L1_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFT4078958.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="ACH3L1_004525"
     CDS             1558..2043
                     /locus_tag="ACH3L1_004525"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFT4078959.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAZXUL010000296         2162 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZXUL010000296 DAZXUL010000000
VERSION     DAZXUL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.298"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            311..505
                     /locus_tag="ACH3L1_004526"
     CDS             311..505
                     /locus_tag="ACH3L1_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078960.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            866..994
                     /locus_tag="ACH3L1_004527"
     CDS             866..994
                     /locus_tag="ACH3L1_004527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078961.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1304..1640)
                     /locus_tag="ACH3L1_004528"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="ACH3L1_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          687 a          444 c          451 g          580 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggctttaa
     1801 ttggaataca tatattacaa cgtattatat ataattggta ttctgggaac tatattctca
     1861 aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga
     1921 attgataaaa aaaacgccag caacacatag tgccggcgga gggagtaccc catggagaaa
     1981 atgtgatgcc tagagcatca tgaatatata attaaaaata gttagcgttg tcaatacttt
     2041 cacaaaaata attttcgtag tatagaaaga tatttttatg catgacctac ctgaatttgc
     2101 tccgggtaga ggttataaat aaaaattgaa tcacgacaaa cacaatattc atagtatggc
     2161 ga
//
LOCUS       DAZXUL010000297         2125 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZXUL010000297 DAZXUL010000000
VERSION     DAZXUL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.299"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..128)
                     /locus_tag="ACH3L1_004529"
     CDS             complement(<1..128)
                     /locus_tag="ACH3L1_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFT4078962.1"
                     /translation="MVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQLLDETFSV"
     gene            complement(67..390)
                     /locus_tag="ACH3L1_004530"
                     /pseudo
     CDS             complement(67..390)
                     /locus_tag="ACH3L1_004530"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            complement(387..920)
                     /locus_tag="ACH3L1_004531"
     CDS             complement(387..920)
                     /locus_tag="ACH3L1_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFT4078963.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(986..1615)
                     /locus_tag="ACH3L1_004532"
     CDS             complement(986..1615)
                     /locus_tag="ACH3L1_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078964.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1619..1834)
                     /locus_tag="ACH3L1_004533"
     CDS             complement(1619..1834)
                     /locus_tag="ACH3L1_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFT4078965.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          545 a          564 c          468 g          548 t
ORIGIN      
        1 accgagaacg tttcgtccag caactggaga gagttcgacg gtggcatcat tgcccatgcc
       61 ggaggcgctg gaggtttcgg ctgaggatgg cacagggcat tttcctttga cgagcacccg
      121 accaccatta tcaagcttgc gccgaagagc atcattttca gctttcgcat cagctaactc
      181 cttcgtgtat ttagcatcga gtgcatcagc atcacgctgg cgctgctgca tgtcagtaat
      241 ggttgcgttc gccagcttca gttctccggc gtttttgtcg cgctgctctt tgtaggtaat
      301 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
      361 agcggagata atcgcggtga ctctgctcat tgatctatcc cccaacaggc taatgcgctt
      421 tcctggtcac gacgaataac ctgtccatag cagttatttg aacgtatgcg gcaatcgcgc
      481 ccaacatctt ttatccacca gcgaatcgcc tcgcatgcgc ccttacgatc accggcattc
      541 agccgcttat aaaacgtcga cgggaaacac ttaccggggc caatgttata ggggcaaaat
      601 gacgctatac ccgctttctg gggttcggtc agaggtactt taatattgcg ctccacccat
      661 gccagcgcct tatcacgttc aatagcgtta acctggtcac atttttcctt cgacagcttc
      721 attcccggta tgacaggctt accatccacc attgtggcac cacgacagat ggtccatatg
      781 ccagaaccat cacggtatgc cgttgtgtga ttaccctctt tttcgttcag aaactggtca
      841 agtatctggg gagcagacgc gcctgcggca atcagcgcca gaacagcagc cgacaggccg
      901 tatttgattt ttgcgctcat ggatatttat caggatttat cggcaacaaa taacgagcca
      961 acttatatac gccttttaag ataagtcagc cccggctgga tccagtcatc aggctctttc
     1021 ttaaaggggg agtattgaaa tcacgaagaa gagcctcccg caccgcagca tccatgtctg
     1081 caccactggc aagtgcttca atctctgccg ccacctgcaa ataccccatg caacggccaa
     1141 tgcgtttcat aactctggtg ttttcgtctt tcaggcatct gatagcgaga ttaataattt
     1201 tcaggcagtg atgatgcgat gcccggacag gttttccgtt ctcataaacg acgaactcac
     1261 cctcttcaca ggaaaccctg aaaggccgat ggggttcctg gtctttatcc ggtgcagacg
     1321 caataaaatc acttttatgc tcttcctctc cggcactgtt aaccgtaatg acgtactcag
     1381 gccgcaggta agccgcctct ttttctgaca atgcggccgg taaaaacttt tcctgcaaaa
     1441 acttcataaa agtctcgtga gcgactttgt ggcagtaatc gtaaatcgcc gcataatgct
     1501 catcgcggcg tttgttttca tcttcagaac tcaccaacgc cgacaatttt ttattaagct
     1561 cagtgatttc attttccagg tgattgaatc gctgattcat ttcttcatgc ttcattattt
     1621 actctccccg tgcagcctta cgcttgtctt ctcttatttt gaaatacaga ttcgtcagat
     1681 aagtcagaag ccccagaagc agacttccca gcacaccaat cgcagcccac tgtgatggcc
     1741 tgacctgatc caaccactgc aaaaaccagt agccggcact gccggcggag gtgccgtagg
     1801 caatgcccgt tgaaattttg tccatggatt tcatagcctc acctccgcaa ataacggatg
     1861 gcgtagtttt acactgagaa atgaaaggga tttgaaaaga aaaaaacgca aaagcgggcg
     1921 aaacgatata tacagtaagg aaagcactct atccaacaaa ccacccacag ttaatcggaa
     1981 taaaagcaga gtgcttatga atgatcgcac gcccgaaggt tagtatttct gcacagcaat
     2041 tttgcaaaaa aaggcgatca ttcataactt aaacgtcttt cagtcactcc gggatttccc
     2101 atcatcgcag actgaaagac tctga
//
LOCUS       DAZXUL010000298         2085 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZXUL010000298 DAZXUL010000000
VERSION     DAZXUL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.300"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..797
                     /locus_tag="ACH3L1_004534"
                     /pseudo
     CDS             1..797
                     /locus_tag="ACH3L1_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            912..1253
                     /locus_tag="ACH3L1_004535"
                     /pseudo
     CDS             912..1253
                     /locus_tag="ACH3L1_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1236..2073
                     /locus_tag="ACH3L1_004536"
                     /pseudo
     CDS             1236..2073
                     /locus_tag="ACH3L1_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          504 c          631 g          435 t
ORIGIN      
        1 tgggccttgc ccgtagcagt tatttttatc accgggctgc actgaaagcc ggtgataaat
       61 acgccacgat acgtaagatg ctgacagata tctttaacag taattaccag tgttatggct
      121 atcgtcgcct gcatgcgatg ctcaggcatg agggggggcg gctatcagaa aaggttgtac
      181 gcagacttat ggtggaggaa cagcttgtcg tcagtcgtaa ccgtcgtcgt cgctacagct
      241 catattgcgg agaaatcgga ccggctccgg ataaccttat cgccagagat tttaaggcgg
      301 agcaacctaa tcagaaatag ctgacagata tcacggagtt ccagctccct gcaggtaaag
      361 tctggctgtc accagtggtg gactgcttcg atggaaaagt tgtgagctgg tctctcagta
      421 cacgccctga tgctgaactg gtcaacacta tgctggatag cgctgtcgaa acgttaaatg
      481 ctggcgaacg accggtgata cacagtgaca gaggtgggca ttatcgctgg ccaggctggc
      541 tggaaagagt gaatgcagca ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg
      601 ataatgctgc atgcgaaggc tttttcggca gactgaaaac ggaaatgtat tatgggcgta
      661 aatggtcggg catcacgcca gaaaagttca tgcagcaagt ggatgcttac atcagatggt
      721 ataacgagcg gcgtataaaa ttatcgctgg gtgccgtcag ccccaaaatg taccgccaac
      781 aatgcgggct ggaatgataa agcagtccag gaaatcgtcc gcatcccccc ttcaccattt
      841 ggattgtccg ctccacccaa catgttgttt ccttaaggtt cctacaccac aaaggaaact
      901 ctcatgctgc gcagtggatg gctgacgttg ccgacaggcg ggaacttcgc cagttcaggc
      961 agacaccgga acagcgcttc acgcaggaac aggagcatct gcagccgctg ctgggtacgg
     1021 acttcgatat ccgtcatgtc tcctgggatg gctatatcga ggttggcggg aaccgttaca
     1081 gtgtgccgga aagcctgtgt ggtcagctgg tatcaatacg gatctcgctg gatgatgaac
     1141 tgcggatcta cagtaatgag caacaggtag catcgcaccg tctctgttcg gctgcatacg
     1201 gctggcagac cgtgccggag catcacgctc cactctggca gcaggcttcg gagcgcatgg
     1261 ccgaatggct gggggagata cagaaacggg taatcactgt ctgtgaccgg gaagcggata
     1321 tctggcatta cctgtattat aaagtcagcc acggacagcg tggtgcgtgc tgcacagaat
     1381 cgccggctgg aagaggcacc cggcaagctc ttcgaactgc cggaagtcct ggcaaccgca
     1441 ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgtca ggcccggatg
     1501 ttcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca ggcgctcccg
     1561 ctcacgtatg tctgttgccg ggagcaggca gaggacggtg cctgctggca tctgctgacg
     1621 tcaggaaaag cggcgagtgc cgccgatgca cgacgtattg tcagccatta cgagcgacgc
     1681 tggctgacag aggaatacca caaggcgtgg aaaagtggtg gtacatggaa tcgctgcgaa
     1741 tgcagacccg ggataacctt gagcgcatgg tggttatcct ggcgtttata gcggtacggg
     1801 tgctgggcct gcggcaggga ggtgtcagtg aagaaacaca aaatgacagc tgtgagaaaa
     1861 tactgacgcc gacagagtgg aaactactgt gggttaagct ggaaggaaag ccgcttccgg
     1921 tgcaggcacc gaccctgaaa tgggcctggg gcgatggcat gacagcaaac gcacaggtcg
     1981 tcccggttgg ggcgtcatgt gggatggctg gtcagacttc aggatatggt tgagggttac
     2041 ctggtgatga agtctcttga tcaggagctc tgatcaagag acagc
//
LOCUS       DAZXUL010000299         2077 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZXUL010000299 DAZXUL010000000
VERSION     DAZXUL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.301"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..483
                     /locus_tag="ACH3L1_004537"
     CDS             <1..483
                     /locus_tag="ACH3L1_004537"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFT4078966.1"
                     /translation="SPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVADPNLDEL
                     YQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGKGLAKKLA
                     LMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCEVRMLREL
                     "
     gene            complement(529..945)
                     /gene="rraB"
                     /locus_tag="ACH3L1_004538"
     CDS             complement(529..945)
                     /gene="rraB"
                     /locus_tag="ACH3L1_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFT4078967.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1107..>2077
                     /gene="argF"
                     /locus_tag="ACH3L1_004539"
     CDS             1107..>2077
                     /gene="argF"
                     /locus_tag="ACH3L1_004539"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFT4078968.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHT"
BASE COUNT          523 a          508 c          523 g          523 t
ORIGIN      
        1 tcacctgtaa tgcgtcgcct gacgctgcag gataatcctg ctatcgccag cgtcatccgt
       61 caggtttacg cagagtatgg tcttaccgcc gataaaggct acaccgttgc cgatccgaat
      121 ctcgacgaac tgtatcaagt gtacagccag cctggccatg cttattgggt ggttgaatac
      181 aatggcgaag tggtcggcgg cggcgggatt gcgccattaa caggtagtga agcggacatt
      241 tgcgaactgc agaaaatgta ctttcttccc gctattcgcg gtaaagggct agcgaaaaaa
      301 ctggccttaa tggcgatgga gcaggcacga gagatgggct tcaaacgctg ctatctggaa
      361 acgaccgctt ttttaaagga agccattgcg ctttatgagc atttgggctt tgagcatatc
      421 gactatgcgc ttggctgcac gggccatgtc gattgtgaag tgcggatgct acgcgaactc
      481 taaaaaaatg cccgctagta tgtgctatcg ggcattttaa tcaattaatt agtggcgaac
      541 cccgtcatcg tcttcatcga caaaatcttc atcgtcgcca tcttcgccgt taggatcttc
      601 aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc tccgccagcg tcatcagttg
      661 ttcaacctgg gcatcgatca gatcggcatt caacgcgcac tcgctgagga tgtcgcagca
      721 aatcacgata tcaccatctt caacttccag ctcttctgga tcggtcactt cgtaaccgag
      781 tttaaacgct tcaactgccg ctttttccag agtttccaga tcgtctgcgg aaagatggtg
      841 ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc agtaattctt caataatcaa
      901 acgtgtttct tcacgctgtt cttccagttg ttccgggttt gccatggctc attcctctta
      961 atgtcctgcc gatactctta ttgtcacaca ccactgacat tgcctccacc tttgtgacaa
     1021 agatttatgc tttagacttg caaatgaata ataatacaca taaattgagt tttaattcat
     1081 tgaggcgtta gccacaggag ggatctatgt ccgggtttta tcataagcat ttcctgaaat
     1141 tactcgattt cacgccagct gaactcaaca gcctgctgca gttagccgcg aagctgaaag
     1201 ctgataagaa aagcggtaaa gaagaagcca aactcactgg taaaaacatc gcgctcatct
     1261 tcgaaaaaga ctcgactcgt acccgatgct ctttcgaagt tgccgcatat gaccaggggg
     1321 ctcgcgttac ttatcttggc ccaagcggta gtcagattgg tcataaagag tcgattaaag
     1381 acactgcccg cgtgcttggt cgcatgtatg acggtattca gtatcgcggc tatggtcagg
     1441 agattgtcga aacactggcg gaatacgctg gcgtgccggt atggaacggc ctgaccaatg
     1501 agtttcaccc cacgcagttg ctggcggatc tcctcaccat gcaggagcat ttacccggta
     1561 aagcattcaa cgaaatgacg ctggtctatg caggtgacgc acgtaacaac atgggcaatt
     1621 cgatgctcga agctgcggcg cttaccggtc tggatttgcg tctggtcgcg ccacaggcat
     1681 gctggccaga agctgcgctg gttacggaat gccgcgccct ggcacaacaa aatggcggga
     1741 atattacgct gacggaagat gtagctaaag gagttgaagg tgctgacttt atctataccg
     1801 atgtatgggt gtcgatgggt gaagcaaaag aaaaatgggc tgaacgcatt gcattactgc
     1861 gtgattatca ggtgaacagc aagatgatgc agttgaccgg taacccggag gtcaaattcc
     1921 tccattgcct gcccgcgttt catgacgacc aaacgacgct tggcaaaaaa atggcggaag
     1981 agtttggcct acatggcgga atggaagtga ctgatgaggt cttcgaatct gccgccagca
     2041 ttgtatttga tcaggcggaa aaccgcatgc ataccat
//
LOCUS       DAZXUL010000300         2071 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZXUL010000300 DAZXUL010000000
VERSION     DAZXUL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.302"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..259)
                     /locus_tag="ACH3L1_004540"
     CDS             complement(<1..259)
                     /locus_tag="ACH3L1_004540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078969.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARC"
     gene            complement(1012..1410)
                     /locus_tag="ACH3L1_004541"
                     /pseudo
     CDS             complement(1012..1410)
                     /locus_tag="ACH3L1_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
     gene            complement(1413..1779)
                     /locus_tag="ACH3L1_004542"
                     /pseudo
     CDS             complement(1413..1779)
                     /locus_tag="ACH3L1_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1781..2071)
                     /locus_tag="ACH3L1_004543"
                     /pseudo
     CDS             complement(1781..2071)
                     /locus_tag="ACH3L1_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          472 a          581 c          488 g          530 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat
      421 tgtccttatt ccactaatac aaactttttg taaagtatat taaatatact tcatttcata
      481 ctgaaatccc cggaagtatt caatcaaaca attacaccat aaaaggaaga gggggcccca
      541 attaagttgt caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca
      601 ttggtactct ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca
      661 ggtccacgtt ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc
      721 gcaggcagta aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg
      781 ctttcgacac atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc
      841 tcacgtccga ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg
      901 atggtttggt gcctgcgcct gcacgccgac accaaggggc cttccctcat ctcttgtgct
      961 gcatggctgc acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc
     1021 atagcaacag taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg
     1081 gcctcgctgg atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta
     1141 gcgtttattc agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat
     1201 cacaccgccc tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg
     1261 ttttccacag cagtctcata aaacgtgtat cactgatcct gcggcagacg gctttcatca
     1321 gcagccggtg atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc
     1381 ccgtgtttcc ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg
     1441 ttcgggccac tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt
     1501 gcacggtaac tgatcgggct gaacttctgg gaggcttttg cgctcagttc ctgacggcgc
     1561 agactggctg ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca
     1621 ggcgtggggc accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg
     1681 ctgacggaac tgcggacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc
     1741 gctgcgggcc acccggagta cgcggcacat tgccttgatg acggtttacg cggtcgcctg
     1801 cggcaacggc tttccggctg acggtagtgt ggaccttttt accccggaga acaccggcaa
     1861 gccccataac cgccatgaga cgtgccacag tgcatctggc cactctgata ccttcccgta
     1921 acaactgatg ccagacttta cgcacagcgt atacctgatg attctcatcg tatacgcgca
     1981 gtatctcttt cttcagccag tcatcgcgct gcgcacgggc actgcgttta tcatgatgat
     2041 gtcgctgttg ctgacagtgg taatacgttg a
//
LOCUS       DAZXUL010000301         2043 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZXUL010000301 DAZXUL010000000
VERSION     DAZXUL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.303"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1936
                     /locus_tag="ACH3L1_004544"
     rRNA            <1..1936
                     /locus_tag="ACH3L1_004544"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          527 a          446 c          645 g          425 t
ORIGIN      
        1 gtgaagaggg aaacaaccca gaccgccagc taaggtccca aagtcatggt taagtgggaa
       61 acgatgtggg aaggcccaga cagccaggat gttggcttag aagcagccat catttaaaga
      121 aagcgtaata gctcactggt cgagtcggcc tgcgcggaag atgtaacggg gctaaaccat
      181 gcaccgaagc tgcggcagcg acgcttatgc gttgttgggt aggggagcgt tctgtaagcc
      241 tgtgaaggtg tgctgtgagg tatgctggag gtatcagaag tgcgaatgct gacataagta
      301 acgataaagc gggtgaaaag cccgctcgcc ggaagaccaa gggttcctgt ccaacgttaa
      361 tcggggcagg gtgagtcgac ccctaaggcg aggccgaaag gcgtagtcga tgggaaacag
      421 gttaatattc ctgtacttgg tgttactgcg aaggggggac ggagaaggct atgttggccg
      481 ggcgacggtt gtcccggttt aagcgtgtag gctggttttc caggcaaatc cggaaaatca
      541 aggccgaggc gtgatgacga ggcactacgg tgctgaagca acaaatgccc tgcttccagg
      601 aaaagcctct aagcatcagg taacatcaaa tcgtacccca aaccgacaca ggtggtcagg
      661 tagagaatac caaggcgctt gagagaactc gggtgaagga actaggcaaa atggtgccgt
      721 aacttcggga gaaggcacgc tgatacgtag gtgaagcgac ttgctcgtgg agctgaaatc
      781 agtcgaagat accagctggc tgcaactgtt tattaaaaac acagcactgt gcaaacacga
      841 aagtggacgt atacggtgtg acgcctgccc ggtgccggaa ggttaattga tggggttagc
      901 gcaagcgaag ctcttgatcg aagccccggt aaacggcggc cgtaactata acggtcctaa
      961 ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg aatggcgtaa tgatggccag
     1021 gctgtctcca cccgagactc agtgaaattg aactcgctgt gaagatgcag tgtacccgcg
     1081 gcaagacgga aagaccccgt gaacctttac tatagcttga cactgaacat tgagccttga
     1141 tgtgtaggat aggtgggagg ctttgaagtg tggacgccag tctgcatgga gccgaccttg
     1201 aaataccacc ctttaatgtt tgatgttcta acgtggaccc gtgatccggg ttgcggacag
     1261 tgtctggtgg gtagtttgac tggggcggtc tcctcctaaa gagtaacgga ggagcacgaa
     1321 ggttggctaa tcctggtcgg acatcaggag gttagtgcaa tggcataagc cagcttgact
     1381 gcgagcgtga cggcgcgagc aggtgcgaaa gcaggtcata gtgatccggt ggttctgaat
     1441 ggaagggcca tcgctcaacg gataaaaggt actccgggga taacaggctg ataccgccca
     1501 agagttcata tcgacggcgg tgtttggcac ctcgatgtcg gctcatcaca tcctggggct
     1561 gaagtaggtc ccaagggtat ggctgttcgc catttaaagt ggtacgcgag ctgggtttag
     1621 aacgtcgtga gacagttcgg tccctatctg ccgtgggcgc tggagaactg aggggggctg
     1681 ctcctagtac gagaggaccg gagtggacgc atcactggtg ttcgggttgt catgccaatg
     1741 gcactgcccg gtagctaaat gcggaagaga taagtgctga aagcatctaa gcacgaaact
     1801 tgccccgaga tgagttctcc ctgacccttt aagggtcctg aaggaacgtt gaagacgacg
     1861 acgttgatag gccgggtgtg taagcgcagc gatgcgttga gctaaccggt actaatgaac
     1921 cgtgaggctt aaccttacaa cgccgaagat gttttggcgg attgagagaa gattttcagc
     1981 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaatta gcctggcggc
     2041 cgt
//
LOCUS       DAZXUL010000302         2042 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZXUL010000302 DAZXUL010000000
VERSION     DAZXUL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.304"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..362)
                     /locus_tag="ACH3L1_004545"
     CDS             complement(<1..362)
                     /locus_tag="ACH3L1_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4078970.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACH3L1_004546"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACH3L1_004546"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFT4078971.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          635 a          493 c          416 g          498 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttgaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aatgctgtaa
     2041 tg
//
LOCUS       DAZXUL010000303         2040 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZXUL010000303 DAZXUL010000000
VERSION     DAZXUL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.305"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(13..901)
                     /locus_tag="ACH3L1_004547"
                     /pseudo
     CDS             complement(13..901)
                     /locus_tag="ACH3L1_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          551 a          475 c          474 g          540 t
ORIGIN      
        1 gtattaagac tatcacttaa ataagtgata ctggttgtct ggagattcag ggggccagtc
       61 tagcagctgc tctacggtca tatttttaat tgcgctccgg tttacaagag cccacccctg
      121 tttttccaga taaaactgga aagtatccag ggtacagacc agtgcaccgt caggaacagt
      181 ttcggtgaat ctgatattgc cgtgttcgtc gaagtgcaca accagagtgc gaccgttacc
      241 cggaatcatc ttgtcagcgg acggggtatt attctggtgc agttctgcct ccatgcggtc
      301 gaacatcagc gatgtaggcc tctttgaatg cagcggcttt tttcccagtg aagcccatca
      361 ccaggaaaac gaagccgttt ttggtgattt ggtacattgg gcgtttttcg cctttggtgt
      421 cggtgtaggt gacgtcctca aaattgaggg cgttaaattc aggtgagcat tcagtatttg
      481 cgatggcacg taacacattg tcgtgtcgtt tgtggaagaa ctctgcaacc gcaacagacg
      541 tagtgacagc gcgaccgttt tcgatggtta cgtcagggtg agaaagggca gggatagtag
      601 ccatgatggc atcctcgagt gataagttaa ttaactcacc accgaggttt tccacgacca
      661 taagggtggt gagacgtaca ggggtggaaa taccggtcac tcgagaaccc ggccagtctt
      721 gcgactgccc tgcacatccc accataattt gaatggagct gtgcattacg cataaaaaaa
      781 ccgcttcagc gcggctatgc gctcgagtaa cgttcgggtt tccacgcccg gcccccgttt
      841 tatgaggtgc agatgcacta taattccacc cgttctggtt ttcaatagct acattcaaca
      901 ttttctctta cctttcatca ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag
      961 gtgacgttaa ccagttaaca attaacgccg gatacaggga aacccataac cacccgatac
     1021 gtgggaacta atcgcttttt aactgttccg cgtgcgctca gccgcattca ccgcatcaca
     1081 aaattcactt taaaaagggc ggacatcagc cagcaatgaa actgatgccg ccaaaggtac
     1141 caatcaacat ggagtgttgt ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc
     1201 cggtgtcctc aacggggaag gaataactcc gccatactta ccgccgcgtc atttcgcgtt
     1261 gtgtgcctgc ttttaactac gtcaggcgag gtggttcctg ttattcccca acaacaagaa
     1321 atttgtataa tctgaatacc ccaacaataa gaagagtgag caagtgatgt attagcgatg
     1381 tcagctctca aagagactgc atcaatgtta aaattattca atgaagcaaa aactcaggct
     1441 gaggttgatc gcgctgtgtc tgaaataaac tctaagttgt caagcattca gcttgaatat
     1501 ttgtcatctc attgatgagc tgacaatctc taaaagcaat gaggctgcgc tcaaagcaaa
     1561 actcacggag atagaaaagt ttaagttcca aagtgaagac tgcgaacctc gctaattgat
     1621 tactggaacg attgtgtatg ccagacagtg caatactagc tctggcaaga ttaccatgta
     1681 tttttgtcca gaatgttttg cgaagagagc aatatctata ttgcaacctt tccctatcga
     1741 ggaaggcgat aaatatacga aaactcaatg cccctcctgt aaaaatgagt atctcatgga
     1801 ctacaatttc aataatgatt tttgagtcac ggtattcaga tttttaaaga acatgccgga
     1861 tactcacccg tgtccggcgc acgcactcca cctcacccgt ggagaactcc ttaattacca
     1921 accttggctt cgttggttag ccattaacgc tggtatgtaa tcattctggc aatgcttaat
     1981 ttgaagtggc acactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa
//
LOCUS       DAZXUL010000304         2036 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZXUL010000304 DAZXUL010000000
VERSION     DAZXUL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.306"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..2036
                     /gene="sat"
                     /locus_tag="ACH3L1_004548"
                     /pseudo
     CDS             1..2036
                     /gene="sat"
                     /locus_tag="ACH3L1_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          748 a          357 c          400 g          531 t
ORIGIN      
        1 catacggcat gatgatttat aaaaatgata aaacatttcg taatcttgaa atattcggag
       61 acagcggctc tggagcatac ttatatgata acaaactaga aaaatgggta ttagtcggaa
      121 caacccatgg tattgccagc gttaatggtg accaactgac atggataaca aaatacaatg
      181 ataaactggt tagtgagtta aaagatacct atagtcataa aataaatctg aatggcaata
      241 atgtaaccat taaaaacaca gatataacat tacaccaaaa caatgcagat accactggta
      301 ctcaagaaaa aataactaaa gacaaagata ttgtgttcac aaatggggga aatgttctgt
      361 ttaaggataa tttggatttt ggttgcggtg gtattatctt tgacgaaggc catgaatata
      421 acataaacgg tcagagattt acatttaaag gagcaggaat tgatatcgga aaagaaagca
      481 ttgtaaactg gaatgcattg tattccagtg atgatgtttt acacaaaata ggccccggta
      541 ctctgaatgt tcaaaaaaaa cagggggcaa atataaagat aggtgaagga aatgttattc
      601 ttaatgaaga aggaacattt aacaatatat accttgcaag cggaaatggt aaggtaatac
      661 taaataaaga taattccctt ggcaatgatc aatatgcggg gatatttttt actaaacgtg
      721 gtggcacgct agatttaaat ggacacaatc agacttttac tagaattgcc gccactgacg
      781 atggaacaac aataactaac tcagatacaa agaaagaagc cgttctggca atcaataacg
      841 aagactccta catatatcat gggaacataa atggcaatat aaaactaacg cacaatatta
      901 attctcagga taagaaaact aatgcaaaat taattctgga tggtagtgtc aacacaaaaa
      961 atgatgttga agtcagtaat gccagtctta ccatgtaagg ccatgcaaca gagcatgcaa
     1021 tattcagaag cacagcgagt cattgctccc tggtatttct ttgtggaacg gactgggtca
     1081 ccgttttgaa agaaacagag agttcatata ataaaaagtt caattctgat tacaaaagta
     1141 ataatcagca gacctcattt gatcagcctg actggaaaac cggggtgttt aaatttgata
     1201 cattacacct gaacaatgct gacttttcaa tatcacgcaa tgccaatgtt gaaggaaata
     1261 tatcagcaaa taaatcagct atcacaatcg gcgataaaaa tgcttacatt gataatcttg
     1321 cagggaaaaa tattactaat aatggttttg acttcaaaca aactatcagt actaatctat
     1381 ccataggaga aactaaattt acaggtggca tcactgcaca taacagccaa atagccatag
     1441 gtgatcaagc tgtagttaca cttaatggtg caacctttct gaataatact cctataagta
     1501 tagataaagg agcaaaagtt atagcacaaa attccatgtt cacaacaaaa ggtattgata
     1561 tctccggtga actgactatg atgggaatcc ctgaacagaa tagtaaaact gtaacgccgg
     1621 gtctccacta cgctgctgat ggattcaggc tgagtggtgg aaatgcaaat ttcattgcca
     1681 gaaatatggc atctgtcacc ggaaatattt atgctgatga tgcagcaacc attactctgg
     1741 gacagcctga aactgaaaca ccgactatat cgtctgctta tcaggcatgg gcagagactc
     1801 ttttgtatgg ctttgatacc gcctatcgag gcgcaataac agcccccaaa gctacagtta
     1861 gcatgaataa tgcgatctgg catctaaata gccagtcatc aattaatcgt ctagaaacaa
     1921 aagacagtat ggtgcgtttt actggtgaca atgggaagtt tacaaccctt acagtggaca
     1981 accttactat agatgacagt gcatttgtgc tgcgtgcaaa tctggcccaa gcagat
//
LOCUS       DAZXUL010000305         2031 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZXUL010000305 DAZXUL010000000
VERSION     DAZXUL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.307"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1824)
                     /locus_tag="ACH3L1_004549"
     CDS             complement(<1..1824)
                     /locus_tag="ACH3L1_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFT4078972.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2031)
                     /locus_tag="ACH3L1_004550"
     CDS             complement(1932..>2031)
                     /locus_tag="ACH3L1_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4078973.1"
                     /translation="RWNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          580 c          431 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagcg c
//
LOCUS       DAZXUL010000306         2005 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZXUL010000306 DAZXUL010000000
VERSION     DAZXUL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.308"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            222..881
                     /locus_tag="ACH3L1_004551"
     CDS             222..881
                     /locus_tag="ACH3L1_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFT4078974.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1082..1459)
                     /locus_tag="ACH3L1_004552"
     CDS             complement(1082..1459)
                     /locus_tag="ACH3L1_004552"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFT4078975.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1526..>2005)
                     /locus_tag="ACH3L1_004553"
     CDS             complement(1526..>2005)
                     /locus_tag="ACH3L1_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFT4078976.1"
                     /translation="ASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRR
                     RVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLE
                     RAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          494 a          495 c          518 g          498 t
ORIGIN      
        1 acgcactttg cgccgaataa atacctgtga cggaagatca cttcgcagaa taaataaatc
       61 ctggtgtccc tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg
      121 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
      181 tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
      241 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
      301 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
      361 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
      421 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
      481 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
      541 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
      601 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
      661 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
      721 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
      781 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
      841 aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      901 ggtgccctta aacgcctggt tgctacgcct gaataagtga taataagcgg atgaatggca
      961 gaaattcgaa agcaaattcg acccggtcgt cggttcaggg cagggtcgtt aaatagccgc
     1021 ttatgtctat tgctggttta ccggtttatt gactaccgga agcagtgtga ccgtgtgctt
     1081 ctcaaatgcc tgaggccagt ttgctcaggc tctccccgtg gaggtaataa ttgacgatat
     1141 gatcatttat tctgcctccc agagcctgat aaaaacggtt agcgccgggg ttggattttt
     1201 cagcgttcca gctaaggcta aggcattcct gttcaagcgc aagccgggct ataaagcgca
     1261 ttatcgcttt acccgtgccc ttatttcgat cgcactgaga aacatacagc tctttaatat
     1321 gcagctgacc gctgtatcgg ggcgagggat aaagaatatt gcaacatgcc aggccagtaa
     1381 tgttattgcc gcagcgcgct ctgatcacca gggtaccgga aagccggttg aataactttt
     1441 tacagagata atccttcatc aacgcttcct gaatgatacc ttcgccataa taggggtcgt
     1501 ctcagaaaac ggaaaataaa gcacgctaag ccggttgcag aggccgtagc ggcctgaact
     1561 tccccgcgcc gatcttggcg ctgctgcgcc ataggtaatc accggtcagg ttgatgtgct
     1621 cccagccgag tggcgacagg tactgcaata gcgagtcatc gacggcatga ccattgccgc
     1681 gcaacgcatg cgccgcacgc tccaggtaga ccgtgttcca cagcacgatg gccgccgtca
     1741 ccaggttgag gccgctggcc cggtagcgct gctgctcgaa actgcggtca cggatttcac
     1801 caaggcggtt gaagaacacg gcacgggcca gcgcattgcg cgcctcgccc ttgttcagcc
     1861 cggcatgcac gcggcggcgt agctcgacgc tttgcagcca gtcgaggatg aacagcgtgc
     1921 gctcgatgcg gcccaactcg cgcagcgcga cggccaagcc gttctggcgc gggtagctgc
     1981 cgagtttcct gagcatcagc gaggc
//
LOCUS       DAZXUL010000307         1969 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZXUL010000307 DAZXUL010000000
VERSION     DAZXUL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.309"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            240..509
                     /gene="yacA"
                     /locus_tag="ACH3L1_004554"
                     /pseudo
     CDS             240..509
                     /gene="yacA"
                     /locus_tag="ACH3L1_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
     gene            509..678
                     /gene="yacB"
                     /locus_tag="ACH3L1_004555"
                     /pseudo
     CDS             509..678
                     /gene="yacB"
                     /locus_tag="ACH3L1_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="ACH3L1_004556"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="ACH3L1_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFT4078977.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1708..>1969
                     /locus_tag="ACH3L1_004557"
     CDS             1708..>1969
                     /locus_tag="ACH3L1_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFT4078978.1"
                     /translation="MTEALRLHFEEHLPRVVAGCRLGVPKSTVCGMFVRFRNAGLSWP
                     LPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRR"
BASE COUNT          560 a          432 c          462 g          515 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgtctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggc
//
LOCUS       DAZXUL010000308         1953 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZXUL010000308 DAZXUL010000000
VERSION     DAZXUL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.310"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..85
                     /locus_tag="ACH3L1_004558"
     CDS             <1..85
                     /locus_tag="ACH3L1_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078979.1"
                     /translation="LIALARRRCDVLFAMMRDGTFYTPQGS"
     gene            complement(102..461)
                     /locus_tag="ACH3L1_004559"
                     /pseudo
     CDS             complement(102..461)
                     /locus_tag="ACH3L1_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(461..1381)
                     /locus_tag="ACH3L1_004560"
     CDS             complement(461..1381)
                     /locus_tag="ACH3L1_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFT4078980.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1519..>1953
                     /gene="pgrR"
                     /locus_tag="ACH3L1_004561"
     CDS             1519..>1953
                     /gene="pgrR"
                     /locus_tag="ACH3L1_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFT4078981.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          483 a          483 c          455 g          532 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacgggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca ccccccacac
      121 acacccagca cgccaatgcg ttcagcatca acatatggca gagttactgc ataatcgata
      181 acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccaacgagg ctacccacca
      241 gattctccct ggaaactggc gtcataagcg agaacgacat agcctttttc agccaatgcc
      301 ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg gacgctgata
      361 atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt ggcagcaata
      421 tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt tcctcaaaga
      481 acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa agatccatat
      541 ggtttgcgcc ttcaacaatg tgatagcgtt tatcctggct tgatgcccga tcgtacaggt
      601 cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc ggctgagtca
      661 ggtacacttc cgccatatgg taagcatcat aggtaataat ctggttaagg ctgcgcaaag
      721 tagcgtaacc cggtgctgtt ggatactgcg cacgaggtgt gtggtaatac tcccaggcct
      781 gacgcagttc ttcgttcggc gcatcggcct ccttcattgg agccagtggc ataatggcgt
      841 attcttcgct gctaatatcg ctggttcggg cgtttgagcc agcttcaatg tgtggcagag
      901 catcaataga tttcacattg ttttcccaac cattgcggaa catcgaacca atattgaccg
      961 cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg gctgtatatc
     1021 ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa gaaagcgttg
     1081 tcaggtaatc aatcacggca ctgacgtctt cagtacgaat gtatgggttt tctaactgac
     1141 gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg acaaatccct
     1201 tttccgccag cttttcggca taggttccgg ccgtttgctc tttaacgccc ccacctgggt
     1261 gagataccac aattgccgga tactgacggg tttcatcaaa ttttggaggg aaatagatca
     1321 ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta ttattcatca
     1381 ttcagttcct catggagttg tcggttcgtt ttgacggttg gtgatatcac tatagatatt
     1441 gatcattaag ttgattagac cctaaatcat gattagacct atcatttaaa tgattaatag
     1501 attgtatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc gtttgtcgtc
     1561 gttgcagagg agcgtagctt cactcgcgca gcagcccgcc tgagcatggt gcagtcagct
     1621 ttaagccaga tagtgcgccg tatagaagaa cgattgggat tgcggcttct gacgcgaacc
     1681 acgcgcagcg ttgtacccac tgaagcgggc gagcatcttt tgtctgttct tggccctata
     1741 ttgcatgaca tagattctgc cctgacatcc ctgagcgatc tgcagaaccg cccatccggg
     1801 acaatacgta ttactactgt agaacatgca gcaaaaacga tattgttacc agccatgcgc
     1861 acattcctga aatcgcatcc tgaaattgat attcagctta ccattgatta tggtttgacc
     1921 gatgtcgttt ctgaacgttt tgatgcaggc gtc
//
LOCUS       DAZXUL010000309         1952 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZXUL010000309 DAZXUL010000000
VERSION     DAZXUL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.311"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..203
                     /locus_tag="ACH3L1_004562"
     CDS             <1..203
                     /locus_tag="ACH3L1_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078982.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="ACH3L1_004563"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="ACH3L1_004563"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFT4078983.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          489 a          484 c          449 g          530 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat ta
//
LOCUS       DAZXUL010000310         1944 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZXUL010000310 DAZXUL010000000
VERSION     DAZXUL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.312"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(35..307)
                     /locus_tag="ACH3L1_004564"
     CDS             complement(35..307)
                     /locus_tag="ACH3L1_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4078984.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            579..881
                     /locus_tag="ACH3L1_004565"
     CDS             579..881
                     /locus_tag="ACH3L1_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFT4078985.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            872..1354
                     /locus_tag="ACH3L1_004566"
     CDS             872..1354
                     /locus_tag="ACH3L1_004566"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFT4078986.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            1422..1944
                     /locus_tag="ACH3L1_004567"
                     /pseudo
     CDS             1422..1944
                     /locus_tag="ACH3L1_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSfl11 family transposase"
BASE COUNT          509 a          422 c          494 g          519 t
ORIGIN      
        1 tgtgaacagg cgtttcagcg gtcggggctg aaaatcacca ccacttcggg tcatccgcct
       61 tcatctccgc ttctgtttcg tataaatcaa aacgacggca ggtatggcag aacgtgacgt
      121 attcgtacgg tgaatccggg tacattttgt ggaaggtttc ccagaaacag accgtgcagg
      181 agggatgacc ggtgataaag tctgcaaata cggacttata agggtgatta ttggctctgg
      241 cgacggcttt cagaacctct ttcaccattc tggtgtggac tttctggtgc tccaggttgt
      301 gtgacatggg aactcattct ggatggttac tctgaaagct catattctgc cacaccccga
      361 tttgcagccg ccaggctgcc gtggttcagg catttcatta tcaaaatcac attaaacggc
      421 cgtaatcaga catgatttgt gcgccgacac agatcatcgt cacaatgttc aggtctctgc
      481 cattgttttt atcaggggat cgcttttccc gtaagtgatt ctgtatagcg ttctgttacc
      541 atttgcgata acattaattg catagcaaat tgttaccaat ggaggttttt atgtctacag
      601 ctgcaagcgt ccgtaaaaca ccgcgagaac atcagattaa catccgggca actgatgagg
      661 aacgagccgt tattgattat gctgcgagct tggtcaataa gaaccgtacc gatttcatca
      721 tggaactggc gtaccaggaa gctaaaaaca ttatcctgga tcaacgttta tttgtgctgg
      781 ataacgaacg ttatgattcg tttattacgc aactggaagc tcctgtgcag aatgcagaag
      841 ggcgtgaacg tctgatggcg gtaaagcctg aatggaaata aatgtcaccg cgccagcatt
      901 gttgacggat gagcatatac ttcagccatt tgactgtgga aatgaggtgc taagtaactg
      961 gttacgtggt cgggctatga aaaaccagat gctcaatgct tctcgcacgt tcgttatttg
     1021 cttggaggat actttacgta ttgtgggata ctactcatta gctactggtt ccgtcactca
     1081 tgccgagctt ggtcgcagtt tgcggcataa catgcccaat cctgtccctg ttgttctgtt
     1141 agggcgtctg gctgttgatg tttgtaccca ggggcatggt ttcggtaaat ggctactaag
     1201 tgatgccatt caccgggttg tcaatctggc tgatcaggtt ggcattaagg ctgttatggt
     1261 acatgcaatt gatgatgacg ccagagcatt ttatgagcgt tttggttttg ttcagtcagt
     1321 tgtggcacct aataccttgt tttataaagt ctgagtctga ggtagcctga gtttaacgga
     1381 cactccttcc tgaaatagaa tggcatcaga aggagccaat aatgagcaga aaaacccaac
     1441 gttactctac ggagtttaaa gccgaagccg tcaaaacggt tcctgaaaat cagctttcga
     1501 tcagtgaagg cgcttcccga ttatctgttc ctgaaggcac tttaggacaa tgggttaccg
     1561 ccgcaagaaa agggctcaat acaccaggct cccgcacagt ggctgagctg gaatctgaag
     1621 ttatgcaact gcgtaaggct ttaaatgaag cacgacttga gcgagatata ttaaaaaaag
     1681 caacagcgta ttttgcacag gagtcgctga aaaatacgcg ttagtcgaac aatggcgaca
     1741 acaattccct gttgaagcga tgtgtcgggt acctggtgta tccaggagcg gttattacga
     1801 ccgggtacag catgccccct cagacagaaa acaaagtgat gagcggctaa aactggagat
     1861 taaggtggca catatccgca ctcgcgaaac atatggaacc cggcggctcc agacggagct
     1921 ggcagataac ggaattatcg tcgg
//
LOCUS       DAZXUL010000311         1942 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZXUL010000311 DAZXUL010000000
VERSION     DAZXUL010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.313"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="ACH3L1_004568"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="ACH3L1_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFT4078987.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACH3L1_004569"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACH3L1_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFT4078988.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACH3L1_004570"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACH3L1_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFT4078989.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT          459 a          504 c          497 g          482 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccacgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ct
//
LOCUS       DAZXUL010000312         1927 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZXUL010000312 DAZXUL010000000
VERSION     DAZXUL010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.314"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..424
                     /gene="wzy"
                     /locus_tag="ACH3L1_004571"
     CDS             <1..424
                     /gene="wzy"
                     /locus_tag="ACH3L1_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HFT4078990.1"
                     /translation="MLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLFGRGFGSFILD
                     RLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSLILFFSITVSI
                     TNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            428..802
                     /locus_tag="ACH3L1_004572"
     CDS             428..802
                     /locus_tag="ACH3L1_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFT4078991.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            865..1803
                     /locus_tag="ACH3L1_004573"
                     /pseudo
     CDS             865..1803
                     /locus_tag="ACH3L1_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          565 a          259 c          369 g          734 t
ORIGIN      
        1 tatgctttac atggtcggat cacgcagtga agattctgac tctgttagat ttaatgattt
       61 atatttttat tataaaaatg ttgatttagc gacgttcttg tttggaagag gatttggttc
      121 atttatatta gatcgattaa ggattgaaat agtacctctt gagatacttc agaaaacagg
      181 cgttattggt gtatttatat cattagttcc tatgttgctt atctttttga aaggctattt
      241 tttaaatagt acaaaaacat cattaatgat gtcgttaata ctttttttca gtattaccgt
      301 ttctataact aatccatttc tttttacacc catgggaatt tttattatag gcgttgtagt
      361 tttatgggta ttttctatag aaaatatcca aattagtaat aacctcactt ctggagcaaa
      421 ataaaggatg ttaaaaatag ggaagttatt gaccagttct ttttttagtt attttttaat
      481 tggaattgtc aatactgctt tacattgggg ggtattttat gcgtgttata ataatttagc
      541 ttttggtcaa ggccgaagta acattgttgg ttttatatgt gcagcaacct tttctttttt
      601 tgcaaatgca agatgctcat ttaaagtatc tgcaaccaag gctagatatt ttatatttat
      661 attttttatg ggggcgatga gttatttatt cggcgtttta tttgatctct tagctttatc
      721 tccaatattt acattgttca ccttttctct ttttagtttg gttttaggat attgcgcatc
      781 aaaatatttc atttttcgtt agtaaacgat ttgttaaaaa cacaattact tgcacattag
      841 ctaaatagtt tatatgaggg gcttatgtat cactctttta atatcgctgt aattattcct
      901 tgttataatg agcagaaggc aatagcaaaa gtcataaatg atttcaaaac gaatattcca
      961 actgcttcta tatatgtatt tgataataat tcgactgaca gtacagcaca agtggcagag
     1021 gatgctggag caacagttca ctctgttcct ctgaaaggca aggggaatgt cgtaagacgt
     1081 atgttctcag atgtagatgc ggatatttat ctaatggttg atggagatga cacttatgat
     1141 gcaagttctg ctccagaaat gattaaccat ctcataaaaa atcaattgga tatggttgta
     1201 ggatgtcgtc aagagaatgg agatcaaaat acatacagaa aagggcatcg ctgaggaaat
     1261 aaattgttga ctggtactgt tcagactatc tttaaaggga attttactga tatgttgtcg
     1321 gggtatcgtg tattttccag acgctatgtt aaatcttttc catgtctatc aaggggattt
     1381 gaaatcgaaa ctgagcttac aattcatgct ttagaattac gtatgaagta tggtgaggtt
     1441 aatacaaaat atggcgagcg atctgaaggg agtgtgagca aactatcaac ttggagtgat
     1501 gggttcaaga ttctgaaaac aataattaaa ctttactcgc ttgaacggcc actatacttt
     1561 tttagtatta tcggcgtttt acttgctgct ttgtctataa ttttaggatt gcccatcatt
     1621 gttgattata ttgatacagg tttggtgaga agatttccga ctgccttttt aactgcctcc
     1681 atcatgttat cgtcaataat ggcatttgta tgtggcatta ttttgcattc aaatacaaca
     1741 actagacgtg aaatgaaggc attattttat ctttctgaaa aaaactataa actgataatg
     1801 taaatagagt attttactat gattgaattg ccgttgtatt tgtatgttgt gtattgtttt
     1861 gcttttggaa tgacattttt tgtcattggt aatattgtat tgtcatcttt ttttaaggag
     1921 ggggtgg
//
LOCUS       DAZXUL010000313         1913 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZXUL010000313 DAZXUL010000000
VERSION     DAZXUL010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.315"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(53..1702)
                     /locus_tag="ACH3L1_004574"
     CDS             complement(53..1702)
                     /locus_tag="ACH3L1_004574"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFT4078992.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAZXUL010000314         1893 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZXUL010000314 DAZXUL010000000
VERSION     DAZXUL010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.316"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            236..454
                     /gene="tdcR"
                     /locus_tag="ACH3L1_004575"
     CDS             236..454
                     /gene="tdcR"
                     /locus_tag="ACH3L1_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFT4078993.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            710..1250
                     /gene="yhaB"
                     /locus_tag="ACH3L1_004576"
                     /pseudo
     CDS             710..1250
                     /gene="yhaB"
                     /locus_tag="ACH3L1_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
     gene            1272..1893
                     /locus_tag="ACH3L1_004577"
                     /pseudo
     CDS             1272..1893
                     /locus_tag="ACH3L1_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          643 a          339 c          311 g          600 t
ORIGIN      
        1 attaactttc attactttcc aaggtatcgt tattacaatt aaacatcttt aaatatcaac
       61 aagttaaagc ataaaaatca gcataaaccc tgatttttat caaaaaaaaa tgacggggat
      121 aaccatattt tatggcgata acatcattcg ttatgtggtt aatacaaaaa aggggctgag
      181 accatatttc aagcaattac cggataatta ccaggcaaaa tttgagttaa atttaatgag
      241 caagttctca aattttatta taaataaacc attttcagcg ataaatactg cggcacgtca
      301 cattttttca cgctatttgt tggagaacaa acatttattt tatcaatatt ttaaaatttc
      361 gaatacaggt attgatcatc tcgaacaatt gattaacgtc aactttttct cttctgacag
      421 gacgtcattt tgtgaatgca atcgttttcc ataaattctt ctcccctcat aggcgacgaa
      481 tagcattttg tgttgaggat cacaaaacga ataattgctg gtcgccgcga taaggtcaga
      541 cgaagtcaac aagggggatt ttcactgtgc tttacatcag cgtaggacac agaatgttgc
      601 ttcgtttact gtaacgccgg gtaaatgagc gttttttgat agtgcgtaag tgtcgtgcga
      661 ggaaaataag catctattat tgtttgtcat gacgacaagg caggcatcaa tgaaaggctt
      721 cccaattgca catatttttc acccttcaat cccgccaatg cacgcagtgg ttaacaatca
      781 caatagaaat attgattatt ggacggtaaa aagaaagttt gcagaaattg tctccaccaa
      841 tgacgttaat aaaatttaca gtataagtaa tgaactccgg agagtattat ctgcaattac
      901 tgcattgaat ttctatcaag gcgatgttcc ttctgtcatg attccgaatc caaccggaaa
      961 atatgagtcc attcatcata gatatttcta caggagaaca tgatgattat atcatacaaa
     1021 cattagatgt aggcactttt gcaccttttg gtgaacaatg tacttgctca gccgtcaata
     1081 aaaaagagct ggaatgtatt aaggagacga tttctaagta ttgcgcaaag ttcacccaaa
     1141 aagaagccat cttaacaccc cctaggcact ttaataaaac ctgcattact tcagattgtt
     1201 ggcaaattct ctttttttca cccgatcatt ttaataatga tttttattga ttatttataa
     1261 aggataaaat aatgttccct gtttcatcaa ttggtaatga tattagtagt gatttagtcc
     1321 gtcgaaaaat gaatgacctt cctgaaagcc ccataggaaa taacctcgaa gcactagcac
     1381 caggtataga aaaactaaaa cagacctcta ttcaaatggt cactttactt aacgcgttac
     1441 aaccaagtgg aaaatgcatt atcactggtg attttcaaaa agaattagcg tacttacaaa
     1501 atgtaattct ttataatgac tcgtctcttc gtctggattt tttaggttat aacacccaaa
     1561 ttattcaacg atcggacaat acctgcgaac ttaccattaa tgaaccgtta aaaaactagg
     1621 aaatatctac aggcaatatc aatgttaatt gcccattaaa agatatttac aatgaaatca
     1681 ggaggttgaa cgtaatcttt agttgtggga ctggagatat agttgatcta tcctctctgg
     1741 acttacgtaa tgtcgattta gattattatg atttcacaga taaacatatg gctaatacta
     1801 ttctaaatca ttttaaattg gattcaacaa attttactaa tgccaacatg tttcaggtta
     1861 attttgttag ttcaacacaa aacgccacaa tct
//
LOCUS       DAZXUL010000315         1849 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZXUL010000315 DAZXUL010000000
VERSION     DAZXUL010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.317"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..257)
                     /gene="ydfV"
                     /locus_tag="ACH3L1_004578"
     CDS             complement(<1..257)
                     /gene="ydfV"
                     /locus_tag="ACH3L1_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFT4078994.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            282..521
                     /gene="relB"
                     /locus_tag="ACH3L1_004579"
     CDS             282..521
                     /gene="relB"
                     /locus_tag="ACH3L1_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFT4078995.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            521..808
                     /gene="relE"
                     /locus_tag="ACH3L1_004580"
     CDS             521..808
                     /gene="relE"
                     /locus_tag="ACH3L1_004580"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFT4078996.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            880..1035
                     /gene="hokD"
                     /locus_tag="ACH3L1_004581"
     CDS             880..1035
                     /gene="hokD"
                     /locus_tag="ACH3L1_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFT4078997.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1253..1504
                     /gene="rem"
                     /locus_tag="ACH3L1_004582"
     CDS             1253..1504
                     /gene="rem"
                     /locus_tag="ACH3L1_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFT4078998.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1571..1849
                     /locus_tag="ACH3L1_004583"
                     /pseudo
     CDS             1571..1849
                     /locus_tag="ACH3L1_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          410 c          467 g          465 t
ORIGIN      
        1 gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag cctgaccgtc
       61 acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa aaagagctga
      121 cctgcacaat acaggatgga cttagcaatg gctgctcctg gcacaaagcg gacagtgatc
      181 accgttctta cgactacttt ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc
      241 gatacttgta atgacatttg taattacaag aggtgtaaga catgggtagc attaacctgc
      301 gtattgacga tgaacttaaa gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc
      361 cttctgaagc gcttcgtctc atgctcgagt atatcgctga caatgaacgc ttgccgttca
      421 aacagacact cctgagtgat gaagatgctg aacttgtgga gatagtgaaa gaacggcttc
      481 gtaatcctaa gccagtacgt gtgacgctgg atgaactctg atggcgtatt ttctggattt
      541 tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg acggtacgtg aacagttgaa
      601 aaagaagctg gttgaagtac ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat
      661 gcctgattgt tacaagatta agctccggtc ttcaggctat cgccttgtat accaggttat
      721 agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa agagaacgct cggaagtata
      781 tagcgaggcg gtcaaacgca ttctctgaac caaagcatga catctctgtt tcgcaccgaa
      841 ggtgacactt ctgctttgcg ttgacaggag aagcaggcta tgaagcagca aaaggcgatg
      901 ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga cggcactggt aacgaggaaa
      961 gacctctgcg aggtacgaat ccgaaccggc cagacggagg tcgctgtctt cacagcttac
     1021 gaacctgagg agtaagagac ccggcgagtg agaaatccct cgccacctct gatgtggcag
     1081 gcatcctcaa cgcacccgca cttaacccgc ttcggcggtt ttttgttttt attttcaacg
     1141 catttgaagt tctggatggt gccggaatag aatcaaaaat acttaagtag cgcgcaggga
     1201 taaaagggat ggaccccgaa caggggagtg ctacttatct ggaaggattc tgatgatgaa
     1261 catcgaagaa ctgcgtaaaa ttttttgtga agatggcctc tatgctgtgt gcgttgaaaa
     1321 tggaaatatt gttagtcatt accgcattgt gtgtttgcaa aaaaatgggg ctgcgttaat
     1381 taattttgtg gatgcccgag tgacggacgg atttatcttg cgcgacggtg agtttgtcac
     1441 ttcattacag gcattgaaag agatcggaat aaaagctggc ttttctgctt tttcagaaga
     1501 ataaactcat ctacaatctt gcgcggggct gaactcccgc tgagtaacac cgtgccaccg
     1561 gagaaaaccg atggcacgca acgcaaaata ttacaatcat gataattcga ccgttcttgc
     1621 ccacacgcac gagcggtatt ctcacgcatt taagtcagac tggtaccagc atccctcatg
     1681 cactgaagaa caggccgaat ggcttattca gtgttaccgc aggcgcggat gcgaggttaa
     1741 aaaagccctt agcctcgact accgtcacta gataatctcc gtcaggctcc cttactccga
     1801 acagccaccg cgtccgtccc gcacattcca gcaacggatc tggaggtaa
//
LOCUS       DAZXUL010000316         1847 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZXUL010000316 DAZXUL010000000
VERSION     DAZXUL010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.318"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(229..630)
                     /locus_tag="ACH3L1_004584"
                     /pseudo
     CDS             complement(229..630)
                     /locus_tag="ACH3L1_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1059..1847
                     /gene="virF"
                     /locus_tag="ACH3L1_004585"
     CDS             1059..1847
                     /gene="virF"
                     /locus_tag="ACH3L1_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HFT4078999.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          572 a          331 c          346 g          598 t
ORIGIN      
        1 ttttcttgtt tttgtggaag gcaagggaat atagtttgtg taagcatatt gcttccttat
       61 gttatatttt agtaaagaat ttatttttta cattatagtc ggctaaatga agtttactgt
      121 aaaaaaacga tacttttttc gtaagcgatg agatgtggag ccagaaaaat ggctgcgtta
      181 cgtcattgaa ctgtccttga cccacgatat tcagacagtc gttgtatctt aagttgacat
      241 tacccgctga cgtcgatatt cccttggtga gagataaccc aacgcactat gcggatggtt
      301 ttcattgtaa tgctcgatcg ccactgcaag gttatgcaat gccgttcttg gtttcggcat
      361 gaacgcgatg cagtcttcct tcatcgtttt catgaaccgt tctgctattc cattactctg
      421 agggctactg actgccgtcg tgcagggctc cagattcaac tctctggcga actgccgtgt
      481 ttcatgagcc gtataagccg aaccgttatc ggccactgga ttggccgttc cggtccttta
      541 tcgccatatc gctcctgaca tgacatcctg caccgttgct ttgtcataat ctcccgtgcc
      601 tgctgcccag tctatggctt cacgatcgca atatggaacg taacgctgtc atcgggagaa
      661 tcgtcaatgc ggcattcggt taacgcttac caatatcaac accaaccgga tttgtggagc
      721 ctccagtctg aaggggcttt atgcgttccg tataggatat tatgatgctg gagttttgcg
      781 aagctccact cctttccgaa catttacaca gatattgcct aagaaaagtg aaaccataat
      841 atggttatag tccctttcag tgcaaatact tagcttgttg cacagagaaa tagaagctgc
      901 ataagctctt tcttcaaaaa atgtaaataa agttaaatat aggaaaaatt acttaatcta
      961 tcttaataac ggaaagattt tgtatacaat cactgttaca gaaatttctt agttactctg
     1021 taaacactaa atatagtttg gtatattctg ttgaatttat gatggatatg ggacataaaa
     1081 acaaaataga tataaaggtt cgcttgcata actatattat tttatatgca aaaaggtgtt
     1141 caatgacggt tagctcaggc aatgaaactt tgactatcga tgaagggcaa attgctttta
     1201 tagagcgaaa tatacaaata aacgtctcca taaaaaaatc tgatagcatt aatccatttg
     1261 agattataag ccttgacaga aatttattat taagcattat tagaataatg gaaccaattt
     1321 attcatttca acactcctat tctgaggaga aaagggggtt aaacaaaaaa atattcctcc
     1381 tctctgagga ggaggtttct atcgatttgt tcaaatctat aaaagagatg cctttcggca
     1441 aaagaaagat ctatagttta gcttgccttt tatcagctgt ttctgatgag gaagctttat
     1501 atacttcgat atcgatagct tcttctctta gtttttctga tcagataagg aagattgttg
     1561 aaaaaaacat cgagaagaga tggcgtcttt ctgatatttc aaataacttg aatttatcag
     1621 aaatagctgt tagaaaacga ttggagagtg aaaaattaac atttcaacaa atccttcttg
     1681 atattcgcat gcatcatgca gcaaagcttt tattgaatag tcaaagctat attaatgatg
     1741 tatcaagact tatcggaata tcaagcccat cttattttat aaggaaattt aatgaatatt
     1801 atggtataac tccaaagaaa ttttacttat atcataaaaa attttaa
//
LOCUS       DAZXUL010000317         1832 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZXUL010000317 DAZXUL010000000
VERSION     DAZXUL010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.319"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..180)
                     /locus_tag="ACH3L1_004586"
     CDS             complement(<1..180)
                     /locus_tag="ACH3L1_004586"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079000.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="ACH3L1_004587"
     CDS             complement(321..875)
                     /locus_tag="ACH3L1_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFT4079001.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="ACH3L1_004588"
     CDS             complement(872..1162)
                     /locus_tag="ACH3L1_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFT4079002.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..1761)
                     /locus_tag="ACH3L1_004589"
     CDS             complement(1162..1761)
                     /locus_tag="ACH3L1_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFT4079003.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          496 c          421 g          470 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cgcatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctgcctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcaaaaaa tcgcaactct ccggcgtcgc cgggagaagt aatccggaag aggtttgttt
     1741 gaccagttgt atatgcgcca tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc
     1801 cgttatgtag tataccagaa ttaatgccaa ta
//
LOCUS       DAZXUL010000318         1809 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZXUL010000318 DAZXUL010000000
VERSION     DAZXUL010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.321"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            173..1198
                     /locus_tag="ACH3L1_004590"
     CDS             173..1198
                     /locus_tag="ACH3L1_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFT4079004.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="ACH3L1_004591"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="ACH3L1_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAZXUL010000319         1786 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZXUL010000319 DAZXUL010000000
VERSION     DAZXUL010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.322"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            129..389
                     /locus_tag="ACH3L1_004592"
     CDS             129..389
                     /locus_tag="ACH3L1_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFT4079005.1"
                     /translation="MAWARHYQQIVREEKETELADDLEKGLPQHLFESLCIDHLQRHG
                     ANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            395..1492
                     /locus_tag="ACH3L1_004593"
     CDS             395..1492
                     /locus_tag="ACH3L1_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFT4079006.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
     gene            1567..>1786
                     /locus_tag="ACH3L1_004594"
     CDS             1567..>1786
                     /locus_tag="ACH3L1_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4079007.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRL"
BASE COUNT          527 a          409 c          449 g          401 t
ORIGIN      
        1 tttagagtaa cgttgggttt ttctgctcat tattagctcc ttctgatgcc attctatttc
       61 aggaaggagt gtccgttaaa ctcaggctac ctcagagcgc ttatccgatt tttgatcgta
      121 ttgaagaaat ggcatgggct cgtcactacc agcagatcgt tcgtgaagaa aaagaaacgg
      181 aactggcgga cgacctggaa aaaggtctgc ctcagcacct gtttgaatcg ctctgcatcg
      241 accatttgca acgccacggt gccaataaac aggcaatcag ccacgcattt gatgatgatg
      301 ttgagtttca ggagcgcatg gcagaacaca tccggtacat ggctgaaacc attgcccacc
      361 accaggttga tattgatttg gaggactaaa atctatggcg ctttttcaac gagcaataaa
      421 cacacaggcc tatctaaaag caggaattat ggggtttgct ggcgacggca aaacctacac
      481 agcaagcgaa ctggctattg gtctggtaat gcttatgcgg caacggggaa ttgaagctgg
      541 taaccgcccg gtaatgtttc tggacacaga aacaggctct gactgggtta aaccccgctt
      601 tgatgcggaa aatatcgaat tatttacggc aaaaacccgc tcgtttgttg atctgcttga
      661 agccattaat gaagcggaaa gcagcggttc cgtaatgatc atcgactcca tcagtcactt
      721 ctggaccgga ttatgcgacg agtatgccag acgccgaaac cgtaaacgag gactggaatt
      781 ttcagactgg gcgtggctca aacaggagtg gcgacgcttt accgatcgat ttgttaacag
      841 tcaggcgcac attattatgt gcgggcgtgc aggatatgag tatgactttt tcgaaggtga
      901 tgatggcaaa cgccagttgg caaaaaccgg tataaaaatg aaagcagaaa cagaaaccgg
      961 ttatgaaccc tccatccgga tccagatgga gaagcaaatg aacatagaaa ccggtcaggt
     1021 gtggcgcacc gccagaatac taaaagatcg ctccacccgt attgatggac aggtattttc
     1081 aaacccaaca tttaaaaact ttcttcctca tatcgagtct cttaacctgg gaggtgaaca
     1141 ccccggcatc gatacctcac gggataacag tgaattattt gccaacgacg gtacaccgac
     1201 atggctaaaa gaaaagcgtg caaaagaaat cgcacttgat gaaattatcg agttgctcaa
     1261 caagcaccat gggggcacca gtaacgacgc caaacgcgca aaagctgatt tgctggaaaa
     1321 aacattcacc tcccgctcat gggaaagaat caaaggtatg gactggccga caatcaaagc
     1381 tggtcgtaat gcactatgga ttgaactgga aggcgttgaa tatgcattcc cggatccaca
     1441 aacacaaaac gaaacgcaac aagcaggata cgatgagaat attccggtgt aaaaccaagt
     1501 gcattttggg aatgaaccgc cccgggaatc ctggagacta aacttcctga gaaagaggta
     1561 aacaggatga ctaaaaatac tcgtttttcc cccgaagtcc gtcaacgggc agtccgtatg
     1621 gttctggaaa gtcagggcga atatgactca caatgggcga caatttgttc cattgcccca
     1681 aagattggct gtacgccgga gactctgcgt gtctgggttc gccagcatga gcgggatacc
     1741 gggggcggtg atggagggct caccaccgct gaacgtcagc gtctga
//
LOCUS       DAZXUL010000320         1760 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZXUL010000320 DAZXUL010000000
VERSION     DAZXUL010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.323"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..264)
                     /locus_tag="ACH3L1_004595"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="ACH3L1_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACH3L1_004596"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACH3L1_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFT4079008.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1164..1349)
                     /locus_tag="ACH3L1_004597"
     CDS             complement(1164..1349)
                     /locus_tag="ACH3L1_004597"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFT4079009.1"
                     /translation="MLPLSVNLMSSGASLAARLQQHWLWYSYNTKTGGVLAYTFGPRN
                     DETCRELLVLFTPFCIY"
     gene            complement(1374..1646)
                     /locus_tag="ACH3L1_004598"
     CDS             complement(1374..1646)
                     /locus_tag="ACH3L1_004598"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFT4079010.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
BASE COUNT          502 a          434 c          319 g          505 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag gactaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg aataccagag ccagtgttgt tgcagccttg ctgccaacga agcgccactg
     1321 ctcatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg
     1381 ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt cagtgtcctg
     1441 gcggtatcac ggccccctgc gccgttatgt gccatttcaa cgatctgctc cttaacgccg
     1501 ggttttctgg cttcataaga gtaagtgagc tggaaaacac gtttacatga gcgacaacgg
     1561 aaacgctcat gccgggaaca actacggcca tgtcgataaa cttcatcgga atgacagcgc
     1621 ggacaatgaa cagtaacggt agccatggaa aaccttaaat accagtagat taccagaaac
     1681 ttctgtcgat tgaagacatc acccgaagtt tgaattcatt cgaatacttt ttacgaggtt
     1741 cttttcgcca gtcctgtaat
//
LOCUS       DAZXUL010000321         1750 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZXUL010000321 DAZXUL010000000
VERSION     DAZXUL010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.324"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            455..1303
                     /locus_tag="ACH3L1_004599"
     CDS             455..1303
                     /locus_tag="ACH3L1_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFT4079011.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1320..1601
                     /locus_tag="ACH3L1_004600"
                     /pseudo
     CDS             1320..1601
                     /locus_tag="ACH3L1_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
     gene            1637..>1750
                     /locus_tag="ACH3L1_004601"
     CDS             1637..>1750
                     /locus_tag="ACH3L1_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079012.1"
                     /translation="MAGIRSTLIKEKNMNTNYEATVATTDNIVHEVYLEGKH"
BASE COUNT          500 a          399 c          411 g          440 t
ORIGIN      
        1 cagcattata tagttctact ctggcgagat acgttggcat attcatttcc ttactggttg
       61 tgtgagaact tcagtaaaga taccaccaaa gcccggaagt ggtgaaataa aaccgggcac
      121 aacacgaagg cgcatttccg gtatccataa agagtcggtc ttgtctgtga aatttaaatg
      181 gtgggagtgc gcttccggtt gtaaataacg acattgctgt gtgaagtacc agttggcggc
      241 atcggtttaa ttgctggctg atgtccgccc tttttaaagt gaattttgtg atgcggtgaa
      301 tgcggctatg cgcacgcggc acagttaaaa gtcatgttag tccttattgg tttgggtggg
      361 aaagccaact gtaattgtta actggttaac gtcacctgga ggcaccaggc accgcatcaa
      421 caaagttcac ttcggtgatg aaaggaaaga gaaaatgttg aatgtagcta ttgaaaacca
      481 gaacgggtgg aattatagtg cacctgcacc tcataaaacg ggtgccggga ttgctacccc
      541 gacgatgact actgcgcata accgcgctca ggcggttttt ttatgcgtca agcacagcca
      601 cattcagatt atggtggggc gtgcagggca gccgcaaggc tggccggttt cggtagtcac
      661 cggttgtagc aaccctgtgc gtctcaccac ccatgagatt gctacctctg gtggtgagtc
      721 atttaaactg actatcgagg ctgccatcat ggctactatc cttacccttt ctcaccctga
      781 cgcaaccatc gaaaatggtc gcgctgtcac tacgtctgtt gcggttgcgg agttcttccg
      841 gaaaatgcac aaaaacgtta ttcagaagat cgaaactctc gaatgctcgc cggagtttaa
      901 ccggcttaat tttaagcccg tcacctatac cgacgccaaa ggcgaaaaac gcccaatgta
      961 ccaaatcacc aaaaacggct tcgttttcct ggtgatgggc ttcactggca aaaaagccgc
     1021 cgcattcaag gaagcctaca ttgctgagtt cgaccgcatg gaggccgaac tgcgccagaa
     1081 taacacccca cctgcagaca agatgattcc gggtgatggt cgcactctgg ttgttcactt
     1141 cgacaaattc ggcaatgtcg aattcaccga aaccgttcct gatggcgcac tggtctgtac
     1201 cctggagact ttccggtttt atctggaaaa acagggatgg actcttgtta accggggcgc
     1261 aattaaaaat atgactgtgg aacaattact ttctttaaaa taaaaatgga gctacggata
     1321 tgaaccctat gcttgattat tgttttacct ctgcacctca gcagaccata acacacaccg
     1381 gaatcaaaaa tttgttatgg ataatcaaaa aaaacctcca gtagaaaggt gatcccgatt
     1441 taattccctg tgcttatggc ctgtgtgaca gacaagtaag ccctgataag gcttacactg
     1501 ctgaacttat ttacatgcac ggaaccatga tgaggaaaaa gaaacgcaaa tattgtagtg
     1561 aaatctgcgc cgaaaaagac cagatggcac acgaacttta attaactgac tattcgaaac
     1621 tgaatttatg ccagcaatgg cagggattcg ctcaacctta attaaggaga aaaacatgaa
     1681 caccaattat gaagccactg ttgcaactac tgataacatt gttcacgagg tttacctgga
     1741 aggaaagcat
//
LOCUS       DAZXUL010000322         1719 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZXUL010000322 DAZXUL010000000
VERSION     DAZXUL010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.325"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..378)
                     /gene="istB"
                     /locus_tag="ACH3L1_004602"
     CDS             complement(<1..378)
                     /gene="istB"
                     /locus_tag="ACH3L1_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFT4079013.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYE"
     gene            complement(375..731)
                     /locus_tag="ACH3L1_004603"
                     /pseudo
     CDS             complement(375..731)
                     /locus_tag="ACH3L1_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            811..>1719
                     /locus_tag="ACH3L1_004604"
     CDS             811..>1719
                     /locus_tag="ACH3L1_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFT4079014.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          444 a          396 c          426 g          453 t
ORIGIN      
        1 ttcatagccc atcgctattg ccagatgggt tttccccaca cctgatggcc ccagtaatac
       61 gatattttca ttacgttcta tgaagctgag tgagcgtaac gactggagtt gcttctgcgg
      121 tgctccggtg gcgaatgtga agtcatactc ttcgaacgtt ttcaccgccg ggaaggctgc
      181 cattcgggta tacatcgcct gtttacgttg atgacgtgcc agtttttctt catgaagcag
      241 atgctccagg aagtccatat aactccattc ctggtctact gcctgttgtg acagcgcagg
      301 cgctgcgctt ataaggcttt ccagttgcaa ctgcccggcg agcgccatca gtcgttgatg
      361 ttgcagttcc atcatcacgc cactcctctg cagaatgagt cgtagatgga gagtggatga
      421 tgctgggggg ggttgtcgaa gctcaccaga ttttcaccag gatgcacgtc atactctttt
      481 ttctccggag gcagtgccag catggactgc tgctcttcga gccagcgatc acagggacgg
      541 gcctggattg tttcatgctt tcgttggtta gcgacatcgt gcagccagcg cagaccgtgg
      601 cggttggctg tttcaacatc gacagtgctc cccatcgggc gcaggcgagt cattagtggg
      661 atgtaaaaac tgttacgggt gtactgcacc atccgttcca ccttaccttt agtctgtgcc
      721 ctgaagggct gaggaatccc cagaaaacga gacaaacata gcattatgtg atctactgat
      781 ttgtgccaac aattcaatga gatcactgct atgcctgttc ataaagtatg tcagaatttc
      841 ttccgggatg ccttagcacc atttcatcag taccgacaaa atgccctgat ggatgccaca
      901 atggcgttaa tcaacggagc atctctgacg cagaccagta tcggacgttt tttgccgggt
      961 aatgcacaag ttaagaataa gatcaaacgt attgatcgcc tcatgggtaa tgaggcgctc
     1021 catcgcgata ttccaatgat tttcaggaat atcacttcaa tgctgacacg gcagttatct
     1081 ttatgtgtaa tagccgtaga ctggagtggc tatccctccc aggagcatca tgttcttcgt
     1141 gccagccttc tgtgtgacgg gcgttccatt ccgttattga gtaaggttgt tccttctgaa
     1201 aaacaaaata atccattgat acaacatgat ttccttgatt cccttgctca atccctgccc
     1261 cccgatgcca gggttattat tgtcacggat gctggatttc agagcgcatg gtttcaccat
     1321 attacttctc tgggctggga ttttattggt cggatcagaa ataacgttca gtattgcctc
     1381 gataacgcgc ctgaaagatg gttaaaagtg tcggatagcc ctgaatgtaa aacaccggaa
     1441 tatatggggg ctggaaggct ggttaaagaa aggaaaaaaa gcatccgggg gcatttttac
     1501 acctataaga aatcggcaaa agggcgaaaa aagaaacgtt cgaaaggtca gagtggttta
     1561 aacaaaacag acaaagaaca gagcaaatca gcaaaagaag cctggttgat cttcagcagt
     1621 acaaatgact tcagagcgcg agaaatcatc aagttataca gtcggcgaat gcagatagag
     1681 caaaactttc gtgatgaaaa gaacgggcgc ttcggattt
//
LOCUS       DAZXUL010000323         1718 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZXUL010000323 DAZXUL010000000
VERSION     DAZXUL010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.326"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(110..577)
                     /locus_tag="ACH3L1_004605"
     CDS             complement(110..577)
                     /locus_tag="ACH3L1_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFT4079015.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="ACH3L1_004606"
     CDS             complement(643..882)
                     /locus_tag="ACH3L1_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFT4079016.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="ACH3L1_004607"
     CDS             complement(1017..1622)
                     /locus_tag="ACH3L1_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFT4079017.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          550 a          378 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagcaa
//
LOCUS       DAZXUL010000324         1695 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZXUL010000324 DAZXUL010000000
VERSION     DAZXUL010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.328"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(4..507)
                     /locus_tag="ACH3L1_004608"
     CDS             complement(4..507)
                     /locus_tag="ACH3L1_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFT4079018.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(552..1515)
                     /locus_tag="ACH3L1_004609"
                     /pseudo
     CDS             complement(552..1515)
                     /locus_tag="ACH3L1_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT          484 a          363 c          342 g          506 t
ORIGIN      
        1 ccattaactg gcctcctgcg caggttgttt cagttgttga atgctttgat taataaaaca
       61 caaggcataa aacatcatta ccactccact taatggcaga atgatatata cgtatcccat
      121 cggtatttgc atagcggcag atacctgtga tgttgccagt gttttatcaa ttaatttcag
      181 gccaccagtc acaatgaccg aaccggcaaa acctaaaatg agaacgttaa tgactatact
      241 aagaagtaat tgtttgcgct tattcagggc gagagcaaat aaatcaatag agagatgtcg
      301 ctgcgcgccg acggtatatg cagcacctaa taatcctacc cagatcatca aaaagcgtgc
      361 cagctcatcg gttaatgtac ttggctggtt aagtacgtag cggctaaata cttgccagac
      421 tacgcataca accagagcaa gcattacggc gacagaaaac gtggcaatga cgcggtctac
      481 cgcaagtttc aggcggttaa atatcattgt aagcatcctg gaaaatcagg gccagaggct
      541 ggcccggaat attattgagc agcgttgtcg aatttctcta acaaagcagc ttgttttgga
      601 tcttttttga agtcgtcaaa cagaggttgc acggctttac ggaatggcgc tttatccact
      661 tttacaattt caccccccat agctttcgcc tgagcacgtg tgtcagcgtc aattttttcc
      721 cacaattttt gttgataagc ttcggatttc ttcgccgctg tttcgaggat ttgttgctgt
      781 tctggggtta atttattcca tgttttggtg gagataacca aaaagtcagg aattgaggcg
      841 tgttcatctt cagaaaaaac tttggcaatt tcaatatgtc gagtctgcat ccaggaaggc
      901 acgttatttt ctgcgccatc tactacacct tgctgcatcg cagtataaac ttcgccaaat
      961 gaaattggag tgggtgaacc acccatcagt tcaatcattt tgatggtggt cggacttggc
     1021 tgaacacgaa ttttcagtcc tttaagatct tctggctttg taatcggttt tttcgcatag
     1081 aaactgcgag tacccgctac ataagctgat aaagcaaaaa agcctttgtc ttttgttgag
     1141 tccataattt ctttaccaac ttcaccaaaa acaacttaat aagaacggca aattatagat
     1201 agcataaata ttatcaaaag actccagatc actggcggaa cctttggtca tatccagtgc
     1261 gccattttgc aacaattcca tagtttctcg cgcattaccc atctggctgc tgggataaat
     1321 acggatcacc atattacctt ttgacagctg ttttacctct ttcgccagct cttcaaaact
     1381 ctgatgtaca acatgacttc tttccaggtt gtgtgctaac tttaacgcca ctttttctgc
     1441 ggcgtttacg ccagtgctaa ccagagccag caaggatgcg cagactaatg agcgggtaaa
     1501 tgacctcatt ttcataaaaa aactccctgt tgaaggatgg aaattggttg atatctaatt
     1561 gtgcttatca gtgatttgtg cgacaaattg gttaacctgt ctgacaactt gtctatgttt
     1621 atcacaactt gaaataaagt cagaaattaa atattaaatt tgtgcgccag gttgtacata
     1681 tttataatca atgaa
//
LOCUS       DAZXUL010000325         1680 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZXUL010000325 DAZXUL010000000
VERSION     DAZXUL010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.329"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(72..148)
                     /locus_tag="ACH3L1_004610"
     tRNA            complement(72..148)
                     /locus_tag="ACH3L1_004610"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Arg,seq:tct)
     gene            complement(150..225)
                     /locus_tag="ACH3L1_004611"
     tRNA            complement(150..225)
                     /locus_tag="ACH3L1_004611"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(190..192),aa:Ile,seq:cat)
     gene            complement(402..1091)
                     /locus_tag="ACH3L1_004612"
     CDS             complement(402..1091)
                     /locus_tag="ACH3L1_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFT4079019.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1088..1447)
                     /locus_tag="ACH3L1_004613"
     CDS             complement(1088..1447)
                     /locus_tag="ACH3L1_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFT4079020.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1447..1635)
                     /locus_tag="ACH3L1_004614"
     CDS             complement(1447..1635)
                     /locus_tag="ACH3L1_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079021.1"
                     /translation="MPFYFRKECPLNSGYLNMVPALRELMQAKHRAQKAVDELVDCVA
                     ELETRVGKFEKLVQEVLR"
BASE COUNT          411 a          470 c          356 g          443 t
ORIGIN      
        1 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctctg cctgctgagc
       61 taagtcggcg ctggcccacc accgaggact cgaacctcgg accgtcaact tagaaggttg
      121 atgctctatc cggatgagct agtggtggtt ggtggccctt gctggacttg aaccagcgac
      181 ctggcgatta tgagtcgctc gctctcacca ctgagctaaa gggccggagg cagaataata
      241 accatatgtc atcacatctg caaactcatc tgaccaccag cacgtttaac gtcctgtgcc
      301 atttttcagg cataaaaaaa ccgctcggcg gcgggttttg cttactttac catcgcgtac
      361 aaaatcggca aagtatcaga tttgcatgag atatatgcct tttaatctac ttttgcaata
      421 ctttgctttg aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa caataaagcc
      481 tcactatcca gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt aaatgttttg
      541 gaccagtttt tggttgttac tcccgccagt aatgccagct cctgatattc ataaccttcc
      601 ccaccaaaaa gttctgcttt taccgcctgc gccgccagcc agattaattt tttcaggcgt
      661 tcctgtgttt tctctgcaat tttcctagtg ccagaatgag ccttaaactc ttcccacgcc
      721 cagtgggtta tggtgatctg atactcccag caaatacttc cgctgtaaca ccacaacaac
      781 caggctttat agtgctcttc aagagacaga acagcccgcc gccacgatga tgtcgaaaac
      841 gcaacctgac tgaccagagg aattgacgcc cccttcgcca gtgattgttt tcccgggatt
      901 ggtggattat cccgcgttat catttttccg gtcacctcat cgcggtaccg gattttttta
      961 cgcctgtaac gccctgtatc gaacatggca ttctcctgcc aggcttcaag ctgacctttt
     1021 gtcgccccac tcaaatcagc ggtggcgata atgagctgct cacgaacaaa ctgtaaatac
     1081 tggttattca tgcacactcc agctctgtga tttttatccc caaccgacca ccaggaacag
     1141 gcagtccgcg cacaatattg gtttcatcaa actgctcgtc gtcgataagc aaccccgcat
     1201 gtgtcagtgc atccagtggt gctttcagga tattgtccag gtcacgacgc cgcttatccg
     1261 gtggctctgc aataatcttt atcgccagtc ttccggacag gcttaatttc agtcgctgct
     1321 gacggacaat aagcgccaca tcccggcgat aacgctcacc ggcttttgat acaaaatatg
     1381 tgctgccacg acgtcgccag taagtattca ccgtcggcgg ataaggtaaa ataaactcat
     1441 ggcgcatcag cgcagcacct cctgcaccag cttttcaaac tttccgactc tggtttccag
     1501 ctctgccaca cagtccacca gctcatctac tgctttctgt gcgcgatgct tcgcctgcat
     1561 cagttcccga agcgcgggta ccatattgag gtagcctgag tttaacggac actccttcct
     1621 gaaatagaat ggcatcagaa ggagccaata atgagcagaa aaacccaacg ttactctaaa
//
LOCUS       DAZXUL010000326         1643 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZXUL010000326 DAZXUL010000000
VERSION     DAZXUL010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.331"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..141
                     /locus_tag="ACH3L1_004615"
     CDS             <1..141
                     /locus_tag="ACH3L1_004615"
                     /inference="COORDINATES: protein motif:HMM:NF020026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFT4079022.1"
                     /translation="SPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEK
                     CF"
     gene            167..874
                     /gene="rfaY"
                     /locus_tag="ACH3L1_004616"
     CDS             167..874
                     /gene="rfaY"
                     /locus_tag="ACH3L1_004616"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFT4079023.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            899..>1643
                     /locus_tag="ACH3L1_004617"
     CDS             899..>1643
                     /locus_tag="ACH3L1_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFT4079024.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEE"
BASE COUNT          608 a          211 c          286 g          538 t
ORIGIN      
        1 tctccgtgga gtaaagaaga tttacttaaa cctgtaaata gcaatcagta tcggtattgt
       61 gcaaaacata aatttaaaca aaagcattat atggcaggca tttttaatta tttaaagtat
      121 tataaagaaa aatgcttcta aagatttaag gttaaaataa ttcattatga tatataataa
      181 aacgataaat gggttgaaag ttttcataaa agataatgat cccttttatg agcaagttct
      241 aaatgacttt ttaacttgca gggttaaaac attaaaagtt tttcgttcta ttgatgatac
      301 taaagtcatt cttattgaca ctgctcgtgg acctcttgta cttaaagttt atgcgccaaa
      361 gcataaaatg accgaacgtt tcttaaaatc ttgcataaaa aaagattatt atgaaaattt
      421 aatttatcaa accgatcggg tacgtggtga aggcatccaa tcaattaatg actatttttt
      481 gctagctgaa cgtaaaacat taaatttcgc tcattattat attatgctca ttgagtatat
      541 tgaaggtgtc gggttaaatg agtatttgga aatttctgaa gatctgaagg atcaattatc
      601 agaatcgata aaagagttgc atcagcatgg aatggtgtct ggcgatcctc ataaaggaaa
      661 ttttatagta tccgaaaaag gtttaagact aattgacctt tcaggtaaaa aaacaaccgc
      721 tgttttaaaa gcaaaagatc gcattgatct tgaacggcac tataatataa aaaatgaatt
      781 gaaagacttt ggctatacat atcttatatt caaaaagaaa attaaaaaag taatcaggga
      841 tgtaaaagtc aaactaggat tgaagagcaa ataatgatta tgatgaaggg taattaaatt
      901 ggattttaaa catcttactc aatttaaaga tataattgaa ctggacaagc gccccgttaa
      961 acttgatgaa cgggaaacgt ttaatgtctc atggggtatt gatgagaact accaggttgg
     1021 ggctgcgatt tcaattgctt caattcttga aaagaataaa caaaacaaat ttacctttca
     1081 cataatcgct gattacttag acaaagagta tattgaatta ttatcacaat tagcaacgaa
     1141 gtatcaaaca gtaattaaat tatatcatat tgattctgag ccattgaagg cgctacctca
     1201 atcaaatatc tggccagtat ctatttatta tcgtttgctt tcatttgatt atttttctgc
     1261 gcgattggat tcattattat atcttgatgc tgatatcgtc tgtaagggtt cattgaacga
     1321 gttaatagca ttagagttta aagatgaata tggggcagtg gtaattgatg tagatgctat
     1381 gcaaagtaaa agcgctgagc gtttgtgtaa tgaggatttt aacggtagct attttaactc
     1441 tggtgtaatg tatattaatt tacgggaatg gttaaaacaa agactaacgg aaaaattctt
     1501 tgatctatta tcagatgagt caattataaa aaaattaaag tacccggatc aagatatttt
     1561 aaacttaatg tttctacatc atgctaaaat attaccgaga aaatataatt gtatttatac
     1621 tataaagtca gaatttgaag aaa
//
LOCUS       DAZXUL010000327         1601 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZXUL010000327 DAZXUL010000000
VERSION     DAZXUL010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.332"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(333..479)
                     /locus_tag="ACH3L1_004618"
                     /pseudo
     CDS             complement(333..479)
                     /locus_tag="ACH3L1_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            533..715
                     /locus_tag="ACH3L1_004619"
     CDS             533..715
                     /locus_tag="ACH3L1_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFT4079025.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(816..1145)
                     /locus_tag="ACH3L1_004620"
     CDS             complement(816..1145)
                     /locus_tag="ACH3L1_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFT4079026.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1317..>1601)
                     /locus_tag="ACH3L1_004621"
     CDS             complement(1317..>1601)
                     /locus_tag="ACH3L1_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079027.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          435 a          368 c          377 g          421 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      361 ggcaggagtt cgtcaacacg gttggagggc cattcaggca gtatgctcag aatatggcgc
      421 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc ggcaacaagc
      481 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
      541 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
      601 gaaaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
      661 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
      721 attggttctc agatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag
      781 ggttgccggg caaccctcat tgaataaaac gggaattact tcgcttcgcc gtttttcatt
      841 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
      901 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
      961 tggattgctt caacgctcat ccctggcttg atgtaatcgc tcaggttgtc cagcaacagg
     1021 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     1081 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     1141 tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt
     1201 agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt ttcactatca
     1261 taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt caggaattat
     1321 ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc cgtttccatg
     1381 ttcagccaca cataaccata gattggtgtt tccacgactt tcaggtcatg gtggttatcg
     1441 agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc aaaaaccggc
     1501 tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatgtgc
     1561 tgggtatcac aaagtttttg cgcgttcaat aacacgaaat g
//
LOCUS       DAZXUL010000328         1597 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZXUL010000328 DAZXUL010000000
VERSION     DAZXUL010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.333"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACH3L1_004622"
                     /pseudo
     CDS             complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACH3L1_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACH3L1_004623"
     CDS             complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACH3L1_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFT4079028.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          474 a          337 c          319 g          467 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtgtttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAZXUL010000329         1580 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZXUL010000329 DAZXUL010000000
VERSION     DAZXUL010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.334"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACH3L1_004624"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACH3L1_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFT4079029.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1580)
                     /locus_tag="ACH3L1_004625"
     CDS             complement(1269..>1580)
                     /locus_tag="ACH3L1_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFT4079030.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          481 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac
//
LOCUS       DAZXUL010000330         1561 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZXUL010000330 DAZXUL010000000
VERSION     DAZXUL010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.335"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            400..1149
                     /gene="phoC"
                     /locus_tag="ACH3L1_004626"
     CDS             400..1149
                     /gene="phoC"
                     /locus_tag="ACH3L1_004626"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HFT4079031.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1204..1308)
                     /locus_tag="ACH3L1_004627"
                     /pseudo
     CDS             complement(1204..1308)
                     /locus_tag="ACH3L1_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1319..>1561)
                     /locus_tag="ACH3L1_004628"
     CDS             complement(1319..>1561)
                     /locus_tag="ACH3L1_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4079032.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          426 a          332 c          316 g          487 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc gccaggtaat
     1381 ttctcgccgt tttgtcgtaa cgcgtggcga tgcggcgata ttctttcagc cgcccaaagc
     1441 accgttcaac gacattacga ttgcagtacg catcacgatc aagctgtgca cgtccatccg
     1501 atgccatttt ctcatttgac tttcggggga ttactgcctt tataccgtta tttttcagct
     1561 c
//
LOCUS       DAZXUL010000331         1556 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZXUL010000331 DAZXUL010000000
VERSION     DAZXUL010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.336"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            624..1223
                     /locus_tag="ACH3L1_004629"
     CDS             624..1223
                     /locus_tag="ACH3L1_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFT4079033.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT          408 a          373 c          353 g          422 t
ORIGIN      
        1 tggcttcacc cgtgttaata tcctggataa ctgtgtttga aggatttctg ttaataatct
       61 gctcctgcaa ctcacctgat gagtattagt ggtaatacat aatcttactc gtaaaagagt
      121 ataccttatg tcattaaaca gtcttccggc gtgttgttca atcctgtacc tctgttactt
      181 atctggtacg ttgatatgtg taatgctacg ttaattgcaa tatctggtta tggctttata
      241 ccttcagcat ttaaaatatt ttattaccgg agaaacaggg aagtggaaag actttcggga
      301 taatgtcagg ccattctgac gttaatcaca gtaccaaata acgagagggt aatggaagtt
      361 aaaacctgcg taagttcctc tgttgttcac tggaaaaagt gattgcccac acgcgttata
      421 aaggcctttc cgctgcagcc tggttaacag agcttcaggc gttatatgcg ctgtatggct
      481 gttacgtatg ctgttctttg cctggttctg atacccgtgg aagtcgttac cggaggaaac
      541 acgttcaccc tactacctga gaacatcttc ttcaaaaatt acggcaacgc ttcttcctgt
      601 gttacagaac acgaggaaga aacatgcaaa cttataacac caatcccgct tacgaaatgg
      661 ctccccagct cctggagcac tttaaccagc atctggattc attattcggg gtctattcga
      721 aactgttacc gttcagaatg gattttgctt accgaaaaaa caccctcagc tacagatgtg
      781 cctgcaggta cgccatgtgc gcggagatgc tgcaactgat taacgaggtg ggagagaaac
      841 tggtcgggta tgcctgggtg atggaatata cagaacgtaa agggcttcac attcattttg
      901 tgggctatct gaacggccag agccacagga gttcgtatct ggtatccaga ttgatgggag
      961 atatctggag gcgggttacc gagggtaatg gttactacca ctggtgccgg tttaataaaa
     1021 attatccggt aaacattaac cacgtcattc actattcaga tcacaaagcg gtgaatgatt
     1081 tacgctatgc catcagctac ctggccaaac gggaacagaa agaatgtggc atcatcctga
     1141 aatgcagcgg gcttcctgaa aaaagtaacc ggggtcgacc acgactggac agccccctgc
     1201 cggggatatg tgctctggtg taaacccctg aacgacaagt acgggctggc gctaatcttt
     1261 gctgtcggtt ccggcggcca cttcagcggc actgcgcatt ttttacggcg tggtcattcc
     1321 gtatgaacca tacggagatt taaccatgac acattcttta ccattccttc cggcaaccga
     1381 tacagcacat cccccaatct ccatctctgc ttactgactg ttttttacgt cagtgggcca
     1441 ttgtgtgaca accacagtgc tgtaacagca accagcctgc tttacccaaa ctccagtcca
     1501 gattttaact gaaccgccat tcacaccctg accacgctgc ccgtaccggg acggct
//
LOCUS       DAZXUL010000332         1535 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZXUL010000332 DAZXUL010000000
VERSION     DAZXUL010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.337"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..475
                     /gene="spa40"
                     /locus_tag="ACH3L1_004630"
                     /gene_synonym="spaS"
     CDS             <1..475
                     /gene="spa40"
                     /locus_tag="ACH3L1_004630"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFT4079034.1"
                     /translation="LAFSILVIILDFVIEFILYMKDMMMDKQEIKREYIEQEGHFETK
                     SRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPFISLIETNQCAL
                     AVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVWLEQVENTH"
     gene            497..730
                     /locus_tag="ACH3L1_004631"
     CDS             497..730
                     /locus_tag="ACH3L1_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator YmgB/AriR"
                     /protein_id="HFT4079035.1"
                     /translation="MGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKDIIN
                     VLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(1337..1535)
                     /locus_tag="ACH3L1_004632"
                     /pseudo
     CDS             complement(1337..1535)
                     /locus_tag="ACH3L1_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          485 a          244 c          308 g          498 t
ORIGIN      
        1 cttggcattt tctattcttg ttattattct tgactttgtg attgagttca ttttatacat
       61 gaaagatatg atgatggata aacaggagat aaaaagagaa tatatagagc aagagggaca
      121 ctttgagaca aagtcgagaa ggcgtgagtt gcatatcgag attctttcag agcagactaa
      181 atctgatata cgtaattcaa aattagtggt aatgaacccg actcatattg caattggtat
      241 ttattttaat ccagaaatag cgcctgcacc ttttatttct ctcattgaaa ctaaccagtg
      301 tgccttggct gtcagaaaat atgcaaatga agttggtata ccgactgtgc gtgatgtgaa
      361 attagctaga aaactatata aaacacatac aaaatatagt tttgttgatt ttgaacactt
      421 ggatgaagtc ctacgtctta ttgtttggct tgagcaggtt gaaaacactc attaataagt
      481 aaaggagatg tgttatatgg gagttaattt ctgtaataaa ataggtattg atcagtctga
      541 atttgaaata gaatcttcca tcattaactc cattgctaat gaggtattga acccaatatc
      601 tttccttagc aataaggata taataaatgt tttactcaga aagatttcat ctgaatgtga
      661 cctggttaga aaagacattt atcgttgtgc tctggagttg gtcgttgaaa aaacacctga
      721 tgatttataa tcattcataa taactctttt gctgatataa atatttttgt tgaagatatt
      781 tatgtagtca caggatcaat tgtagaaaat tgttatgttt ttgttaggaa ctgcactaaa
      841 taatatatga taagacagca aaaacgactg accataatcc ttcttttatt aggagttgat
      901 aagagagact attcatcctg taatgttaaa actctgttat atagtattcg cgattatgca
      961 aagtctgtaa atgatcatga gatactaacc gagagtaatc gtttattatc tcattgcatt
     1021 tcagatagta acggagcttt ctttaaaagt agtaaatacg ttccccttaa gtacttacgt
     1081 aaaaggagaa tagcacgaaa gataccaaat gattagcact ttgctagtaa gcttgctggt
     1141 aaactatatt gaccgttggg tttgttttga gactgtaatt taaattgtgt aattgcctgt
     1201 ttttgatatg tttactccaa caacggagac aggtaaatta tggacgaaaa gaagcttaaa
     1261 gcccttgcgg ctgaactggc taaaggcctc aaaactgagg ccgacctcaa tcagttttcc
     1321 cgtatgctga cgaaatatcc cggctcttaa aacgatagtg gctcaggctt tcatttttca
     1381 gcgttcccca gaagatttcc atcggagcgt tgtcgtaaca gttaccttta cgcgacattg
     1441 atgttttcag accagactgc tcctgtatga cccggtaatc gtatgcgcag tactgtgaac
     1501 ctcgagcgga gtggtggatt agcccggcag gtggg
//
LOCUS       DAZXUL010000333         1516 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZXUL010000333 DAZXUL010000000
VERSION     DAZXUL010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.339"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            157..1077
                     /locus_tag="ACH3L1_004633"
     CDS             157..1077
                     /locus_tag="ACH3L1_004633"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4079036.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGFCF"
     gene            1097..>1516
                     /locus_tag="ACH3L1_004634"
     CDS             1097..>1516
                     /locus_tag="ACH3L1_004634"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFT4079037.1"
                     /translation="MLLAGYLSVRLFRNSKPLTNTLFLALTIILLIAYWYVPSGNEYL
                     WLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAGAASGLSGTFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEK"
BASE COUNT          374 a          292 c          348 g          502 t
ORIGIN      
        1 ttctatgatc atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa
       61 ctaatgagtg tgataatgca atactttgag tgattatata atgataagta aacaggtttg
      121 cgacaattgg ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag
      181 gaaattgaaa gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg
      241 gtctttatca gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt
      301 gtgttatccg gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt
      361 tttttatcct atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca
      421 aaagtatttt tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat
      481 attcccggga tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg
      541 atccaaggta tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg
      601 gaaaggatca cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc
      661 cccgttctga ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg
      721 gccgcattta tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt
      781 caggtggaca gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc
      841 gtttcacacc cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc
      901 agaaaacata tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt
      961 tatattctga gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat
     1021 gtcggtggta aggatatggg aaaactggca tcaatggggg gggggttctg tttttgaaat
     1081 cggtggtgtg gctggtatgc ttcttgccgg atacctgtct gtcagactgt tcagaaactc
     1141 aaaaccatta acaaatactt tatttctggc acttaccatt attttattaa tcgcgtactg
     1201 gtacgtgcca tcaggcaatg aatatctctg gctaaactat acaatcctta ttctgttggg
     1261 gctggcagtg tatggtccgg tgatgtttat tggtctttat tcaatggagc ttgttcctaa
     1321 agaagctgcc ggagccgcat cagggctgtc aggcacattt tcctatatct tcggctcaat
     1381 tgttgcgaca ctgggaatgg gacttgttgt cgattatctg ggatgggggg ccacatttat
     1441 cgtactgata ctgtctgctg tttttgccat cattttcaca ctgatgagtc gtgaacgctc
     1501 actggagttc gaaaaa
//
LOCUS       DAZXUL010000334         1484 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZXUL010000334 DAZXUL010000000
VERSION     DAZXUL010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.340"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..649)
                     /locus_tag="ACH3L1_004635"
     CDS             complement(<1..649)
                     /locus_tag="ACH3L1_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFT4079038.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTT"
     gene            complement(813..908)
                     /gene="ymiC"
                     /locus_tag="ACH3L1_004636"
     CDS             complement(813..908)
                     /gene="ymiC"
                     /locus_tag="ACH3L1_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFT4079039.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1022..1189)
                     /gene="yciX"
                     /locus_tag="ACH3L1_004637"
     CDS             complement(1022..1189)
                     /gene="yciX"
                     /locus_tag="ACH3L1_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFT4079040.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1192..1332)
                     /locus_tag="ACH3L1_004638"
     CDS             complement(1192..1332)
                     /locus_tag="ACH3L1_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFT4079041.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          402 a          357 c          340 g          385 t
ORIGIN      
        1 tagtggtgtg cgagtcagta ccaacgagtg tatccggata agcaatccat tcaccgtcct
       61 gcaattcgct ccacactgct ttgccgagat attcgaggtt aacctgatgg caaatgcctg
      121 tgcctggcgg cacgacgcta aaccgactga acgcttgctt tccccatttc aggaacacat
      181 aacgttcgtg gttgcgctcc atttccaggc gtacgttttc ttcaaatgcc tcatcatcac
      241 caaaacgatc gacggtcacc gagtggtcaa tgaccaggtc gaccggtgag agcgggttaa
      301 cctttgcagt atcgccaccg aggcgtttaa ccgcttcgcg cattgccgcc agatcaacaa
      361 cggcaggtac gccggtaaag tcctgcatca gcacccttgc cgggcggtag gcaatttcac
      421 ggtcagcatg ggcatttttc agccatcccg ccagtgcgtg gatatcctct tcggtaactg
      481 agttaccatc ctgccagcgc agcaggtttt cgagcaaaac tttgagtgac ttgggtagac
      541 gggtgatatc gcccagtgat ttagcagcaa gcggcaggct gtagtagtgg taagttttat
      601 ctttggcctg caacgtgtcc ttactggctt ctcgtagggt tgacgacata gctcctcctt
      661 aatgacaggg ttgcgaagcc ctgattctct tcagggtgaa tattaaagat aacacaaaca
      721 tcgcgtaacg atttgataac aacccaaatt gataaaagag gagaaacctt gtggcaaagc
      781 aaatgacaac cccgccgcag cggggtcaag gatcagtgag taatgatcca gaggagttcg
      841 gcccagaaga gcatcgacag agaaaacgcg cccatccagg accaatattt catatttgtg
      901 ttgatcatag gctacaccct taacggttat tattttgtgt tgagataact caagctgtta
      961 ttgctgcggt gtattaaaag ccctttaatt cattaaaaat gggtaataag aatgatgata
     1021 attattgttt cggctgatca tcttcagcaa acagttctat aaaggcttgc tgctgcgatg
     1081 ttagttgcca gtcgtctgga ataacgacgg aacattcccg tttcgtttct ggttcattgt
     1141 cactatgctg gcataatggt gacgactcca gttgctcctg accgaccata gttatcccca
     1201 cactttccaa atcagcagtg cgacaaccac ccagaaaagt gcagaaccaa gaaaaaccgc
     1261 cagccaggct ttacgtttta attcaggatc acggcggggt tcctgatttc cagaaggcat
     1321 tgctaacctc atataatcgc catcacttat cagcaagacg ttgaatgatt ggtgtaatta
     1381 atcatacgat gagacaaatc ctaaagaaac tttagtcaaa aatccagcga atttacgaat
     1441 cgtctccagt gaataattca atcttttgac ctgaaataga tatt
//
LOCUS       DAZXUL010000335         1460 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZXUL010000335 DAZXUL010000000
VERSION     DAZXUL010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.341"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..246)
                     /locus_tag="ACH3L1_004639"
     CDS             complement(<1..246)
                     /locus_tag="ACH3L1_004639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079042.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEK"
     gene            698..982
                     /locus_tag="ACH3L1_004640"
     CDS             698..982
                     /locus_tag="ACH3L1_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFT4079043.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            982..1257
                     /locus_tag="ACH3L1_004641"
     CDS             982..1257
                     /locus_tag="ACH3L1_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFT4079044.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          396 a          366 c          361 g          337 t
ORIGIN      
        1 tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg caaaaacagc
       61 agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa caagtcccat
      121 tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca gccctaacag
      181 aataagttta cgcgtaaatc gccctgaatc cagaagagca ttaagtatca cataaacagg
      241 ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta gtccaccggc
      301 atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact ggtgtgagcg
      361 gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg cccgctaacg
      421 cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc gcgcacagag
      481 gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac tggtgatatt
      541 cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc tacatgtaac
      601 caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg tctgaaagta
      661 atcactatga ttaaatatga ttaacaacta atcgggtatg caaatgaaaa acaataccgc
      721 acaagcaaca aaagtaatta ccgcgcatgt gccattacct atggctgata aagtcgacca
      781 gatggccgcc agactggaac gctcccgggg ctgggttatc aaacaggcgc tttctgcatg
      841 gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa gccctggacg atgtgacatc
      901 cggacaggtt atcgaccatc aggctgtaca gtcctgggcg gacagcctca gtactgacca
      961 tctgttaccg gtgccacgct gatggaactg aagtggacca gtaaggcact ttctgatttg
     1021 gcgcggttat atgattttct ggtgctggcc agtaaacctg ctgccgccag aacggtgcag
     1081 tccctgacac aggcgccggt cattctgtta actcatccac gtatgggaga acagttgttt
     1141 cagtttgaac ccagggaggt cagacggatt tttactggcg agtacgaaat ccgttacgaa
     1201 cttactggcc agactattta tgtattgcgc ctgtggcaca cacgagagaa caggtagatt
     1261 gggtgtggag gcaactttaa catagcctgc aggtattaat tctggagcat tgttatcagg
     1321 ccagatattt tgcattccat taaataatct tgtaggagat aaaataaatt acgatgaaat
     1381 aacgaaaatt accgctcgtg actggcggca atatcgcgct ccgggctggc aaataaccca
     1441 tcagaaacgt tattgccaga
//
LOCUS       DAZXUL010000336         1450 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZXUL010000336 DAZXUL010000000
VERSION     DAZXUL010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.342"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(212..718)
                     /locus_tag="ACH3L1_004642"
     CDS             complement(212..718)
                     /locus_tag="ACH3L1_004642"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079045.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(864..>1450)
                     /locus_tag="ACH3L1_004643"
     CDS             complement(864..>1450)
                     /locus_tag="ACH3L1_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079046.1"
                     /translation="LIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIE
                     FSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLF
                     PQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFT
                     INPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
BASE COUNT          559 a          282 c          210 g          399 t
ORIGIN      
        1 tcctgtggga ttacctcaca ctcgggatta ctaatagtcc aatcactata tcttgctaat
       61 ccaaagacgt caccaattgc catcccttta tagaagtaaa taccatcctc caagcctaat
      121 tggataagat tatctccata taactcacta tattcacttg ctttcttcat cagttcatag
      181 ctatagcgac tttctaagcg tgaattaaaa ttcaagtatt cagaataaaa atatttgaat
      241 tcaaaataca acgatcgccc cgaaaaaaaa taatacacac aaggataaaa tatcgtccca
      301 acttgtaatt tgtaatgttg atatctttta ttaaacttgc aattgagatc gcaacctgca
      361 atactgacaa tgggtaaatg ggtgataaat acctgtcacc ttgcgtaaca gaaaaccaaa
      421 ataagacata tgttaaaaca atcgagaaaa atacccaaag tattttatta ttcctaagtg
      481 taaagacaga cagtatagca taaaaaacaa catcactgcc aactttatta attgcttgtt
      541 gcaaatctaa tgtggcagga cttataccat gtcgagcttg ttctgctgtt tgaacttgta
      601 gatcaatttc attccatagg tagtgaccaa atatatttcc tccagcagga ctaaaaggat
      661 caccagagat ccaaaaacta cggacatacc aataaatgcc accaagaatt gttgccaagc
      721 taaaaataag aaatgtctgt atgtttctct ttttcgctat caatataaca aatatgatgg
      781 ctccatatat ggcaccaaat agttttacgc cacaagcaat tccaataaaa aaaccacata
      841 aaaaaattaa ttcattttgt gttttattgt tttctgagcg aatatatata agaatactgg
      901 ctgcagcaaa acaaagatag cgttaaatca atatatgcat aaccaagatg atctttaagt
      961 ggattgatag taaatagtag gattgtagca ataatgcctg gataataagt tttcgtaatt
     1021 tcccatgaac agccaacaag tccacaggct attacaaaaa aacagatatt tgctaaaaac
     1081 tgagctaacc ttacatctcc taactgtaaa cctaaagcca ttaaaagatc tgcattttgc
     1141 gggaacagag gaaaacgcaa atattgttca acaacaattg catggtgctc aactaatgaa
     1201 cgagccaaag gtaagtgata catagtatca tcccaatcac cgggagctcg cagggacgat
     1261 aacaatgtaa ccacaaaaat caaaacaaga aataaaataa caaacagcgt actgaactca
     1321 atattattta ttgccagcca tctaaattgc tgacgtttaa taatgcttaa aacccaacac
     1381 accgtaaaaa ttaaaaagat agcaatctta ttaaattgcc cgaccatgcc cagaaagtaa
     1441 attatcaaaa
//
LOCUS       DAZXUL010000337         1434 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZXUL010000337 DAZXUL010000000
VERSION     DAZXUL010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.343"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            431..600
                     /locus_tag="ACH3L1_004644"
                     /pseudo
     CDS             431..600
                     /locus_tag="ACH3L1_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1434
                     /locus_tag="ACH3L1_004645"
     CDS             746..>1434
                     /locus_tag="ACH3L1_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFT4079047.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          347 a          331 c          445 g          311 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggt
//
LOCUS       DAZXUL010000338         1421 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZXUL010000338 DAZXUL010000000
VERSION     DAZXUL010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.344"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            41..445
                     /gene="stpA"
                     /locus_tag="ACH3L1_004646"
     CDS             41..445
                     /gene="stpA"
                     /locus_tag="ACH3L1_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFT4079048.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(492..1016)
                     /gene="ygaP"
                     /locus_tag="ACH3L1_004647"
     CDS             complement(492..1016)
                     /gene="ygaP"
                     /locus_tag="ACH3L1_004647"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFT4079049.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1026..1325)
                     /locus_tag="ACH3L1_004648"
     CDS             complement(1026..1325)
                     /locus_tag="ACH3L1_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFT4079050.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          385 a          332 c          336 g          368 t
ORIGIN      
        1 aaatactttt ttgttttggc gttaaaaggt tttctttatt atgtccgtaa tgttacaaag
       61 tttaaataac attcgcaccc tccgtgcgat ggctcgcgaa ttctccattg acgttcttga
      121 agaaatgctc gaaaaattca gggttgtcac taaagaaaga cgtgaagaag aagaacagca
      181 gcagcgtgaa ctggctgagc gtcaggaaaa aattagcacc tggctggagc tgatgaaagc
      241 tgacggaatt aacccggaag agttattggg taatagctct gctgctgcac cacgtgctgg
      301 taaaaaacgc cagccgcgtc cggcgaaata taaattcact gatgttaacg gtgaaactaa
      361 aacctggacc ggtcagggcc gtacaccgaa gccaattgct caggcgctgg cagaaggtaa
      421 atctctcgac gatttcctga tctaattact gaggccggat gtcgctgcta gggcgcgtcc
      481 ggcgtcgctt ctcaagctcg ttggttccac ggcatcttat ctaacaacct tgccattcca
      541 caaaaaccgc tgattcctgc aaacagtaac ccggcaccaa caaagccact taataagaag
      601 aaaccgctat ttacggtata acccagtaca acgccgttta atattaaacc acccgcagcg
      661 atctgcacct ggcgcattaa cggcaagggt tgagatttat ttaccgctac tggcaatcca
      721 gctcttttcc agccatcaat cccatcttcg agtaaaaata tttctgcggg ggcggcaatc
      781 gctgctaatt tatcggcgtt attactggtg cgtttacctg cctggcagtg gaatataatt
      841 tgctcgcgac gtaatttagg tggaagacct gactgttcca gcacggataa tggagccaga
      901 tctgcttcag gaatatgttc acgaagatat tcatcagcgt cacgaatatc gattaacttt
      961 gcgccgcgtg cgattaattc ttgtgcatca tgcggcgaaa tggttgtcaa agccatagtg
     1021 actccttacg gacaatagac atttttcagg gtggcgataa tggcatttac cgcctcattt
     1081 ttaatggaat atagaatgcg ttgggcatcc cgttggctgt tgataagccc ttcgtcccgc
     1141 atacgagcga gatgctgtga tgtcgcagag gcgctcagtc cggtaatgcg cgtcagctct
     1201 cccgcgctgg tgccggggga accgctaagc atgcacagaa tcagcaaccg tttagggtgg
     1261 ctcattgctt tcaataaggc cgctgcctgt tcggtactgg cctgtaattg cgcgagttca
     1321 gtcatattta tttaagtatt ttctaaatta agtaaactct aaactaaaaa tgcaacatat
     1381 accagcctca gcagcgtaaa tgagagtaaa agcgtaagct g
//
LOCUS       DAZXUL010000339         1416 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZXUL010000339 DAZXUL010000000
VERSION     DAZXUL010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.346"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(12..705)
                     /gene="ospZ"
                     /locus_tag="ACH3L1_004649"
                     /pseudo
     CDS             complement(12..705)
                     /gene="ospZ"
                     /locus_tag="ACH3L1_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            761..913
                     /locus_tag="ACH3L1_004650"
                     /pseudo
     CDS             761..913
                     /locus_tag="ACH3L1_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          428 a          277 c          242 g          469 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 ggaagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgccatactt ctggttatac cagagatata cttcttgtca tatatgtttg gtgattcatt
      601 aaagtaacca tgttcaactc taggataaat atttctcact atatattcag tatttgcagt
      661 atttattgga aatacgttct taatattctt gatgggacta atcattttaa tctctatact
      721 caaatataaa cattaccatg aacaatataa ttgttcctaa gtggaatgtc tccacggaga
      781 acactttatc tatcgggaaa tagtgcgggc aacagtgttt aattatatcg agtgcaatta
      841 caatcgctgg cgacggcacc gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca
      901 gaacctcgct taggcctgtg tccatattac gtgggtaggc tcacagagcc tcacttacga
      961 ctgcgtccat attatgtggg taggatcaat aaagacgttt tatatgtgtc tgcttgcagc
     1021 cagaaaattt ataccctgca ggtatcataa acatgcttga taacttaatt ggggctccct
     1081 cttcctttta tggtgtaatt gtttgattga atacttccgg ggatttcagt atgaaatgaa
     1141 gtatatttaa tatactttac aaaaagtttg tattagtgga ataaggacaa tagcttatcc
     1201 aaactgccaa gcctctttat tataagtaag atctggcact ggaatattct caaaaggact
     1261 aacatcatca ccacgacctt caaccaacca ttttattgca tttaaatccc agaatagttc
     1321 agtagtggtc aatttgggaa acgcacaata ttgtacggtt acgttatcgc aatagagtga
     1381 actctcgccc tatacggcgt tttcaaggat tagtcc
//
LOCUS       DAZXUL010000340         1404 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZXUL010000340 DAZXUL010000000
VERSION     DAZXUL010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.347"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..167
                     /locus_tag="ACH3L1_004651"
     CDS             <1..167
                     /locus_tag="ACH3L1_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFT4079051.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="ACH3L1_004652"
     CDS             152..790
                     /locus_tag="ACH3L1_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFT4079052.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGQTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="ACH3L1_004653"
     CDS             1018..>1404
                     /locus_tag="ACH3L1_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079053.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          408 a          257 c          347 g          392 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggcaga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DAZXUL010000341         1385 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZXUL010000341 DAZXUL010000000
VERSION     DAZXUL010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.348"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(87..>1385)
                     /locus_tag="ACH3L1_004654"
     CDS             complement(87..>1385)
                     /locus_tag="ACH3L1_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFT4079054.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLVLM"
BASE COUNT          464 a          324 c          306 g          291 t
ORIGIN      
        1 acaactgcgg cgcaatgggg tgattaagta actcatgtta agtgcctttg ccggataata
       61 taaaaagtta tccggcaaat tattttttac attagcacta gtagctgtgg aatgaacaag
      121 gatatttgcg gaatgtaagt gatcatcatc aacgctacta gcaatgcagc ataaaatagc
      181 agcaatggtt tgattaaatg ttgaatcttc acacccgaga tagaacaacc aacaaatagc
      241 gcactaccca ccggcggtgt taacagccca atacaaaggt tggccaccat cataataccg
      301 aaatgtactg ggtccattcc cagctcttgt gcaatcggca agaagatcgg tgtaaagatt
      361 agtacagccg gtgtcatgtc cataaagatg ccgacaatta ataagacgat attaatcagc
      421 agcaatatga tcagtgggtt atcagaaatc cccatcaatg cgtcgctgat catataagga
      481 atatcagcat tagtcattgc ccaggacatg cccacggaaa aaccgatcaa cagtaataca
      541 atcgaagtgg taacaaccga ctccagaatc agtttgggta aatggcgcca ttttacttcc
      601 cggtaaatca gcaccgagag aataaatgtg tagacaacgg caattgctga cgcttcagtt
      661 gcggtaaaaa tgccgcccag aatgccgccc atcacaatga acaccagaag caaacttggt
      721 aaggcatcca gaaacgcttt acaggcctga gcaaaggttg ctctttctga aagcgggtag
      781 ccacggcgtt tagcaataat gccgcagact atcattaccg caagacccat taagatccct
      841 ggcaaatatc ccgccataaa cagcgatgct acagaaacgc cgccagctgt cagggaaaac
      901 acaattaaca cgtttgaggg cggtatcagt aatccagtaa tacaagatga gacgtttaca
      961 gcggtagaaa atgccggatc ataaccttct ttggtctgaa tgggattcag tgtgccacca
     1021 acggcagcag ctgccgcaac ggctgaacca gaaatggaac caaacatcat attcgccagg
     1081 acgttcacat gaccaagcga accaggaacg cggccaacca ttacctgggc gaggttaatc
     1141 aaacgtatcg caataccgcc gctgttcatc agtgttccgg caaagatgaa aaaggggatt
     1201 gccaacaggg caaagttatc cagtccatta gccagtcgtt gcgaaacagt gattgccgcg
     1261 atatcccagg gaaacatcaa aagcattgaa gctacagtgg caataccgat tgagaacgaa
     1321 atgggcacac caataaagac cagcacaaaa aaacagccaa acagtgtaat cgcgatataa
     1381 ctatc
//
LOCUS       DAZXUL010000342         1381 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZXUL010000342 DAZXUL010000000
VERSION     DAZXUL010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.349"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..89)
                     /locus_tag="ACH3L1_004655"
     CDS             complement(<1..89)
                     /locus_tag="ACH3L1_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4079055.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEG"
     gene            complement(427..>1381)
                     /gene="ospC4"
                     /locus_tag="ACH3L1_004656"
     CDS             complement(427..>1381)
                     /gene="ospC4"
                     /locus_tag="ACH3L1_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HFT4079056.1"
                     /translation="QTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDF
                     GANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQG
                     KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEY
                     HIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADI
                     ALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVN
                     KKFQKVNSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          405 a          268 c          228 g          480 t
ORIGIN      
        1 ccttcactga tcgaaagctg attttcagga accgttttga cggcttcggc tttaaactcc
       61 gtagagtaac gttgggtttt tctgctcatt attggctcct tctgatgcca ttctatttca
      121 ggaaggagtg tccgttaaac tcaggctacc tcactcccgt aaagacagga gtcaagttcc
      181 cgctccgaca taactgcgtg caatggattt tgaaggaacc gtcagcccat tccccggagg
      241 caaacatggg ctggcaaaag tgtagcggta ttaggcacag ctatttagga aagagggacc
      301 atctccatct cattaaatgg accacgccag acaattcact gtcatgctga tgctgcactg
      361 tggggacaga atcactcatg atgacttcga taatcgacga cattattatt tggccgagct
      421 tttaggtcat atctcaaatc gtttgcctaa tattgctcca ttttttaata aaaaatcaat
      481 catttttgaa tctttgcttt tcattgcatt gtcaagcata gtgtccccac tatttacttt
      541 ttgaaatttt ttgtttacat caaccaatcc attattgata aaatattcta gcactttata
      601 gttggcacca tctttactaa gtaagtattc aacatcatac agattacaat atctattgtt
      661 tttcattttc tccatctctg cgacatcttg ttttgtaaaa ttaaatttag atagtaaata
      721 aagtgctata tcagcttttt tattgcctaa agaataagca agggcctgat gagccatttt
      781 tttatcagtc actttattgt taatctcttc ataattagat gctttaatcg catcctccaa
      841 agatatgtct ctgagtttta tttttttgaa gttatctgta cttagcattc ttggtatatg
      901 atactcaagc tgaaacacaa agtttataat tctatccaga ctaacaggat caatattttt
      961 gttataacaa aactctctga atccttgatc tttactcttt cttataaaat caataagatg
     1021 caacccaatt ccaagcctca tatctttagt gttaaatatt ggaacatcag ttttcccctg
     1081 attaccatgc acatacctgc taacttcatc cacaacctct ttaaagttat ctaagaaaaa
     1141 tgattggtgc tcatggtaaa aaacaggtgg aatagcatta tcaaagtgat cggttaatgt
     1201 catatatcca tatggagagt catgatcaat gatatacgca ttggcgccaa aatctacagt
     1261 atgatgtgtt ttatttagtg ggagtttttc ttgatgattg gaaatttcga ccccaaaaaa
     1321 aacaaaatcg tggttagaga tttctttaat atcctcagag tatgtattct tatgtgtttg
     1381 t
//
LOCUS       DAZXUL010000343         1355 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZXUL010000343 DAZXUL010000000
VERSION     DAZXUL010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.350"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            78..398
                     /locus_tag="ACH3L1_004657"
                     /pseudo
     CDS             78..398
                     /locus_tag="ACH3L1_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
     gene            complement(445..786)
                     /gene="symE"
                     /locus_tag="ACH3L1_004658"
     CDS             complement(445..786)
                     /gene="symE"
                     /locus_tag="ACH3L1_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFT4079057.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(1007..>1355)
                     /locus_tag="ACH3L1_004659"
     CDS             complement(1007..>1355)
                     /locus_tag="ACH3L1_004659"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFT4079058.1"
                     /translation="PSLSKVFLAEYNGLCSADMYPLDCYINPNYLLKYILSIPFLMQV
                     KKAENRIKMPKLNSDSFYNIIVAIPPYNEQQAIFDKINSIEAVCNGLISYIGIYHKTQ
                     LHLADALTDAAIN"
BASE COUNT          373 a          257 c          322 g          403 t
ORIGIN      
        1 tttttctgct cattattagc tccttctgat gccattctat ttcaggaagg agtgtccgtt
       61 aaactcaggc tacctcagtg ttattcgatc atcttcgcga tgaagttatg cgcctggacg
      121 cagggatcac gcaggaagta ttgaagctat atattgcgtt taaagcggaa acgaattttg
      181 ttgatgttgt gccgcaaaaa agccgactgc gattgtcgct taatatgcag tttcatgaac
      241 tggtcgatcc gaaaggtatt gccaaagatg tgacaaatgt tgggcgctgg ggtaatggcg
      301 atgtagaaat tggtttcagc gacctcgcac aacttcctta cattatggga ttaattcgtc
      361 aggcatttga aaaacagatg gagagcgcgt tggtataacg cggcgaataa tacccggtag
      421 cactggcgct accgggtcaa tgtatcaggc gactttctgt ttacctgtaa ttaccccaat
      481 aaattcctgt acctgccttt gtttacgcgc cgacagctta cacaccttgc gtagtgactg
      541 catcagttcg ctctcttcgg cggcggcggg ttgggcggtg aggacaatac agccttccat
      601 cactttgaca tctaccgccg tgccggtggc aaaaccggcg gcttccagcc actgaccttt
      661 cagggtgatg gcgggaatac ggctgtaatc cgggtaacga ctcgcataac tcacggttaa
      721 ttgacgatta tttgccgggg agacttctgc ttctaacggt tgtgcaatag aatgcgtgtc
      781 agtcatgatt actattctct ataaatagtg attgtgatta gcggtgcgag tgtgttggcg
      841 cacatccgca ccgtgctaaa tacctggata catcatcagt aaatacaggg aaagtccagc
      901 taaaaattaa tgaaaataag atgaaaatta gcgtgaaata taaattttgg tgatgtgaat
      961 aaagcggcta tttagccgct ttattggttt tgtgagggcg agggaattag tttatcgctg
     1021 cgtcagtgag tgcgtctgca agatgcagtt gggttttatg ataaatccca atataagaaa
     1081 taagaccatt gcatactgct tcaatagaat ttatcttatc aaatattgct tgttgttcat
     1141 tatagggggg aatcgcaaca ataatattgt aaaaactgtc agaattaagt tttggcattt
     1201 ttattctgtt ctctgctttt ttgacttgca taagaaatgg aatggaaagg atatatttca
     1261 ataagtaatt tggattaatg taacagtcta atggatacat atcagcactg caaagaccat
     1321 tatactctgc taaaaaaact ttggataatg acggg
//
LOCUS       DAZXUL010000344         1353 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZXUL010000344 DAZXUL010000000
VERSION     DAZXUL010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.351"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..141
                     /locus_tag="ACH3L1_004660"
     CDS             <1..141
                     /locus_tag="ACH3L1_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4079059.1"
                     /translation="RNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRL
                     RN"
     gene            complement(178..309)
                     /locus_tag="ACH3L1_004661"
     CDS             complement(178..309)
                     /locus_tag="ACH3L1_004661"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079060.1"
                     /translation="MARSAKSRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFD"
     gene            391..584
                     /locus_tag="ACH3L1_004662"
                     /pseudo
     CDS             391..584
                     /locus_tag="ACH3L1_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            727..948
                     /locus_tag="ACH3L1_004663"
                     /pseudo
     CDS             727..948
                     /locus_tag="ACH3L1_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(969..1031)
                     /locus_tag="ACH3L1_004664"
                     /pseudo
     CDS             complement(969..1031)
                     /locus_tag="ACH3L1_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          414 a          304 c          303 g          332 t
ORIGIN      
        1 cgtaatgtcg ttgaacggtg ctttgggcgg ctgaaagaat atcgccgcat cgccacgcgt
       61 tacgacaaaa cggcgagaaa ttacctggcg atggtgaaac tgggctgcat ccgactcttt
      121 tatcaacgct tacgtaatta agggacacag cctagtctcc aggattcccg gggcggttca
      181 atcaaagcgc agaacttctg cgtcttcttc gtcaaaaaaa tcatcttcgt gaagcttcac
      241 gacatagcgg ggaagtttgc ttctttgaga ggcgggttta cgtttacggg atttagctga
      301 acgggccata taaccacctg aaagacaatg acattgcctg tttttataac ggtaattgca
      361 gaccatgaca aaccgcagcc gtcaggctgc ctactcgagt cacaaggctc tttgtgtccg
      421 atatgccggc agcgcattac ccatgaaact ggctggaacg tacaccataa ggtgaagaaa
      481 gttatggggg cggtgaagaa ttatctaacc ttgtcttgtt acatccaaat tgccatcgac
      541 agttacacag ttgtgaagcc ggctctctca aaaagggctt ataaaggctt gagcggtgta
      601 ccgggaaacc ggtatgcacc gttcttaggg gagggcagtc cagcaatgaa ctgtccttac
      661 ccgacaaaca tccaaaacga acggaacgtg ctggaatccg catataaccc gttgtaaagt
      721 gaggaaatgg acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg
      781 caacaggaca ctatccacca gatggctgaa tacaaccgcc tgctctcaca gcggatggcg
      841 gcttatgctt ccgaaatcaa ccgactgaag gcgctggttg cgaaactgca gcgtatgcag
      901 ttcggtaaaa gctcagaaaa acttcgcgca aaaaccgaac ggcagattcc attttcacgg
      961 gcgatctacg ccacacaggc tctgggttgc agtgactgct ccattaaggc gattttgaac
     1021 tcataaggga agtaacaaaa aaactgcaaa agtacaataa ttatatatgg ccaaactaac
     1081 ctctattaaa aaacatcagt ttttcacact tagttgagct tcatgaaaaa atcatcgagg
     1141 atttcattgc agaatacaca ttccactaac tggaagcact ccataagagg aatcagagaa
     1201 agtatcgttt ttttatagta aaattcattg ctgttcaata aaattgatat agttattaaa
     1261 ttatgaaaat aatttcacaa aaaagattaa cttgagttct taagcatcgg attttagata
     1321 ttttgaaata tctggcatca cataacatca aaa
//
LOCUS       DAZXUL010000345         1336 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZXUL010000345 DAZXUL010000000
VERSION     DAZXUL010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.352"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACH3L1_004665"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACH3L1_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFT4079061.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="ACH3L1_004666"
     CDS             complement(590..1255)
                     /locus_tag="ACH3L1_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFT4079062.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          314 g          349 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgtgtga ctgctc
//
LOCUS       DAZXUL010000346         1330 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZXUL010000346 DAZXUL010000000
VERSION     DAZXUL010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.353"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(41..100)
                     /locus_tag="ACH3L1_004667"
     CDS             complement(41..100)
                     /locus_tag="ACH3L1_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFT4079063.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1265)
                     /locus_tag="ACH3L1_004668"
                     /pseudo
     CDS             complement(339..1265)
                     /locus_tag="ACH3L1_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
BASE COUNT          266 a          354 c          321 g          389 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca
//
LOCUS       DAZXUL010000347         1307 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZXUL010000347 DAZXUL010000000
VERSION     DAZXUL010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.354"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..119
                     /gene="ospE1"
                     /locus_tag="ACH3L1_004669"
     CDS             <1..119
                     /gene="ospE1"
                     /locus_tag="ACH3L1_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HFT4079064.1"
                     /translation="PAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            509..>1307
                     /gene="ipaH1.4"
                     /locus_tag="ACH3L1_004670"
     CDS             509..>1307
                     /gene="ipaH1.4"
                     /locus_tag="ACH3L1_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HFT4079065.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGP"
BASE COUNT          454 a          263 c          224 g          366 t
ORIGIN      
        1 tacctgctgc cagccggacg aaaacgttga aattcactgc tacagaaatg caatggttga
       61 caaagataaa tgaagccgga attgatgaaa aacaaagcca acggtattct gatttctgaa
      121 gttaatttca gactcgttta agctccattt tcaaaaaaaa tatatcaggg tttgttacta
      181 ataacttctg aaaacagaaa aaaatagagc agtaattcta tttcagagag gagtaactcc
      241 atctatcctc gatacgtact gaacgttatc gtgatatatt catcatgctt tcttcaccat
      301 tattcgagta tagggagaga cgcaatgacc ttaaatacct ttttatggct gaattaccat
      361 tctcaggaat aacagcagac tccttcccat agacggctta gggggaaagc aatacccgga
      421 gaaagggtac aaagcaaact gttttaaaat taataagcca tattctattt aactagaatt
      481 cttatcgttg ataagaattc tagttataat gattaaatca accaatatac aggcaatcgg
      541 ttctggcatt atgcatcaaa taaacaatgt atactcgtta actccattat ctttacctat
      601 ggaactgact ccatcttgta atgaatttta tttaaaaacc tggagcgaat gggaaaagaa
      661 cggtacccca ggcgagcaac gcaatatcgc cttcaatagg ctgaaaatat gtttacaaaa
      721 tcaagaggca gaattaaatt tatctgagtt agatttaaaa acattaccag atttaccgcc
      781 tcagataaca acactggaaa taagaaaaaa cctattaaca catctccctg atttaccacc
      841 aatgcttaag gtaatacatg ctcaatttaa tcaactggaa agcttacctg ccttacccga
      901 gacgttagaa gagcttaatg cgggtgataa caagataaaa gaattaccat ttcttcctga
      961 aaatctaact catttacggg ttcataataa ccgattgcat attctgccac tattgccacc
     1021 ggaactaaaa ttactggtag tttctggaaa cagattagac agcattcccc cctttccaga
     1081 taagcttgaa gggctggctc tggctaataa ttttatagaa caactaccgg aattaccttt
     1141 tagtatgaac agggctgtgc taatgaataa taatctgaca acacttccgg aaagtgtcct
     1201 gagattagct cagaatgcct tcgtaaatgt tgcaggtaat ccattgtctg gccataccat
     1261 gcgtacacta caacaaataa ccaccggacc agattattct ggtcctc
//
LOCUS       DAZXUL010000348         1293 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZXUL010000348 DAZXUL010000000
VERSION     DAZXUL010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.355"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..109)
                     /locus_tag="ACH3L1_004671"
     CDS             complement(<1..109)
                     /locus_tag="ACH3L1_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFT4079066.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVD"
     gene            complement(102..992)
                     /locus_tag="ACH3L1_004672"
     CDS             complement(102..992)
                     /locus_tag="ACH3L1_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HFT4079067.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(1022..>1293)
                     /locus_tag="ACH3L1_004673"
     CDS             complement(1022..>1293)
                     /locus_tag="ACH3L1_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFT4079068.1"
                     /translation="SFNILNVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVP
                     YLALLYFMVEKYGLLGAAISWSIRVILDALLLQWLYTRMCSVYEN"
BASE COUNT          431 a          245 c          180 g          437 t
ORIGIN      
        1 tgtctacatc tttttgttca ataatacttt ttaattgttc ctcaatccag cattcgccat
       61 tatacgtagc cataagtaca gctactcttt tttgatcaga actatgcatt ataacgaccg
      121 cccccagtaa ttcctcttat tccatccagc gtagccaaaa tatgtttttt catatattca
      181 attttatttt tttgagttgc gataattaaa gtcgtatgaa ttaaagaaaa aacaacttcc
      241 ctaatagaga gtaaaagagg cacatgattt tttctaagca aaagaaatgc atttcttact
      301 cgataataac gacgtactgg cgagtgaatt ggtatgttaa tcccacagat ttttactgac
      361 ttatccccaa tagcatgaac catttctata tcaggaatga tatgaactaa tatgccatag
      421 tttaagcatc taagacacca ttctgtatcg acataatcaa taaaaagttc ctcatccatc
      481 attccaacaa tctcaaaaac ttcttttgaa accatggttc cggatgagat tgttactgaa
      541 cttttaaacg gtgtctgtcc ttcagtgaca tgaattttct ctcgaagacc attttttttg
      601 atattacaaa taggataata atgtgactta tctctatcaa taaaaactgg gccagctatt
      661 tttgcatttt cattatccat atagcaggaa agttttttga taaactcatt gcgaatacgg
      721 ctatcttgat caaaaaaaat tatcttctct gccccttttt caagagaata tcgaatccca
      781 atattttgtg cttttgctat accttcattc ctaccaagac aaattaaatg aactttttca
      841 atgtctgcta atttatcttt caaggtgaaa ttagtcctat tatcgactac tacaacgtaa
      901 caattttgct cctttagttc agtgatcagt gcattcagat ttttaagttc gggattatat
      961 gtcacaatga cagcgtaaat attactattc attatccaag tgactcagta attggttaat
     1021 tttagttttc atatacagaa cacattctag tataaagcca ttgtaataac agagcatcta
     1081 aaattacacg tatgctccag gatattgcgg cacccagtaa cccatatttt tcgaccataa
     1141 aatacaaaag ggctaaataa ggaaccaatt cagcacaatg aatcaaagca gtaattttag
     1201 attttcctaa tgcctggatt gcagagaatg gtatcattgc cagcgcattg aagaaaaaac
     1261 cgatcaataa cacatttaat atattaaagg aat
//
LOCUS       DAZXUL010000349         1277 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZXUL010000349 DAZXUL010000000
VERSION     DAZXUL010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.356"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACH3L1_004674"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACH3L1_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFT4079069.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          229 c          286 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAZXUL010000350         1246 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZXUL010000350 DAZXUL010000000
VERSION     DAZXUL010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.358"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..241
                     /locus_tag="ACH3L1_004675"
                     /pseudo
     CDS             1..241
                     /locus_tag="ACH3L1_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            258..776
                     /locus_tag="ACH3L1_004676"
                     /pseudo
     CDS             258..776
                     /locus_tag="ACH3L1_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            1143..>1246
                     /locus_tag="ACH3L1_004677"
     CDS             1143..>1246
                     /locus_tag="ACH3L1_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079070.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQK"
BASE COUNT          374 a          285 c          276 g          311 t
ORIGIN      
        1 ggtgtatatc cgcactcgcg aaacatatgg aacccggcgg ctccagacgg agctggcaga
       61 taacggaatt atcgtcggtc gtgacagact ggcaggtctt cgtaaggagc tgaggctgca
      121 ctgtaagcag aaacgcaagt tcagggcgac cacaaactct gaccacaatc tgccggttac
      181 gccgaatctg ctgaatcaga actttacgcc cacagcacct aatcaggtct gggtcgcaga
      241 ttcagttgtc caggccttcc gaaatcaacc gactgaaggc gctggtcgcg aaactgcagc
      301 gtatgcagtt cggtaaaagc tcagaaaaac ttcgcgcaaa aaccgaacgg tggatacagg
      361 aagcacagga gcgaatcagc gcacttcagg aggaaatggc ggaaacactg ggtgagcaat
      421 atgacccggt actgccatct tccctgcgcc agtcttcagc ccgtaaaccg ttaccagcct
      481 cacttccccg tgccccccgg gtcatccggc cggaagagga atgctgtccg gcctgtggtg
      541 gtgaactcag tccactggga tgtgatgtgt cagagcagct ggagcttatc agcagcgcct
      601 ttaaggttat cgaaaaacaa cgccctaaac tggcctgtcg ccggtgcgac catatcgtgc
      661 aggcaccggt gccttcaaaa cccattgcac gcagttatgc cggagcgggg cttctggccc
      721 atgttgttac cggaaagtat gcagaccatc tgccattata ccgccaaagt gatctactat
      781 tccacacagc tatttagctc tctttttgtt gagctcccat gattctttca tttattgtta
      841 ctaattattt tatgttatca tgatgttaga acacgctctg ctctgtgtct ctattttatc
      901 tatcatattc tgaggattaa ctacataaca aggcgcaaga tatatataca aaaacaaata
      961 ataccgttgc aataattcct tttagtaaag gaggtgtgat taatttcagg ggatttcaga
     1021 gtaaaaattg tacaacatat taaaatatgt tcatttcgtt ataatacaca caaggaaata
     1081 ttaagttaga taatatattc taaataacca tagataaaaa cgcacataat ggggaatatt
     1141 ttatgaaaat acctgaagca gtaaatcata ttaatgttca gaataatatt gatcttgttg
     1201 atggaaaaac gaatccgaat aaagcaacaa aagcattaca aaaaat
//
LOCUS       DAZXUL010000351         1233 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZXUL010000351 DAZXUL010000000
VERSION     DAZXUL010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.359"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACH3L1_004678"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACH3L1_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFT4079071.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          365 a          206 c          294 g          368 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gtt
//
LOCUS       DAZXUL010000352         1223 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZXUL010000352 DAZXUL010000000
VERSION     DAZXUL010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.360"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            90..>1223
                     /gene="pdeF"
                     /locus_tag="ACH3L1_004679"
     CDS             90..>1223
                     /gene="pdeF"
                     /locus_tag="ACH3L1_004679"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFT4079072.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT          273 a          273 c          278 g          399 t
ORIGIN      
        1 atagtgatga ataatcatca ttcgaagtca ggtgggatgc ctgtctgaat acacctcctt
       61 caggatgtgg gggattctgc tgagcatcta tgaaactgaa tgcaacttat ataaaaatac
      121 gtgataaatg gtgggggctt ccgctgttcc tgccttcttt aatcttgccc attttcgccc
      181 acattaatac tttcgcgcat atttcttccg gtgaggtttt tctcttttat ctgcctttgg
      241 cactgatgat cagcatgatg atgtttttca gctgggcggc attgccaggg atcaccttag
      301 ggatttttgt ccgcaaatat gcagagctgg gtttttacga aacgctctca ttaacggcta
      361 attttattat cattatcatt ctctgttggg gcggttacag ggtctttact ccccggcgta
      421 acaacgtttc acatggtgat acccgtttaa tttcccagcg tatattctgg cagatagtgt
      481 ttcctgcaac gctgtttctg atacttttcc agtttgctgc atttgtagga ttactggcga
      541 gcagagaaaa tctggtcggt gtcatgcctt ttaacctcgg gactttaatc aattatcagg
      601 ccttactggt gggtaatctg atcggtgtcc cgctgtgcta cttcatcatt cgggtagtgc
      661 gaaatccatt ttatttacgt agctattatt cgcaattaaa acagcaggtt gatgccaaag
      721 tcaccaaaaa agagttcgcg ctctggctac tggcattagg tgctttattg ttgctgttat
      781 gcatgccgtt aaatgaaaaa agcacaattt ttagcaccaa ttataccttg tcattattgc
      841 tgcccctgat gatgtgggga gcaatgcgct atggttataa gctgatttcg ctgctctggg
      901 cggtcgtgtt gatgatcagc atccacagct atcaaaatta cattcccatt tatcctggct
      961 ataccacgca gctgaccata acctcctcca gttatctggt attctctttt attgtcaatt
     1021 atatggctgt actggcaact cgtcagcgag cggtagtcag acgcattcag cggcttgcgt
     1081 atgtggaccc ggtggttcat ctgccaaatg ttcgcgccct gaatcgcgcg ttgcgtgatg
     1141 ccccctggtc tgcgctttgt tatttacgca tccctggcat ggaaatgctg gttaagaact
     1201 atggcatcat gctgcggatt caa
//
LOCUS       DAZXUL010000353         1220 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZXUL010000353 DAZXUL010000000
VERSION     DAZXUL010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.361"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            175..>1220
                     /locus_tag="ACH3L1_004680"
     CDS             175..>1220
                     /locus_tag="ACH3L1_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFT4079073.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          398 a          278 c          193 g          351 t
ORIGIN      
        1 atatgtaaaa catcaaaaaa agatgaacca aatatacgaa aaaagcaaat atacttacta
       61 gggtagaaag catgatataa cactcattcc cttatggcga atattctgaa acaccctcct
      121 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
      181 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      241 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      301 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      361 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      421 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      481 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      541 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      601 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      661 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      721 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      781 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      841 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      901 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      961 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
     1021 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
     1081 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1141 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1201 gaggagaatt cccgggcatt
//
LOCUS       DAZXUL010000354         1210 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZXUL010000354 DAZXUL010000000
VERSION     DAZXUL010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.362"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..507
                     /locus_tag="ACH3L1_004681"
     CDS             <1..507
                     /locus_tag="ACH3L1_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFT4079074.1"
                     /translation="SAGYLHASTNAPGSDDLNGINVKYRYEFTDTLGLVTSFSYANAE
                     DEQKTHYSDTRWHEDSVRNRWFSVMAGLSVRVNEWFSAYAMAGVAYSRVSTFSGDYLR
                     VTDNKGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGF
                     IVGVGYKF"
     gene            571..>1210
                     /locus_tag="ACH3L1_004682"
     CDS             571..>1210
                     /locus_tag="ACH3L1_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFT4079075.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSE"
BASE COUNT          285 a          287 c          394 g          244 t
ORIGIN      
        1 agcgcggggt atcttcatgc ctcgacgaac gctcccggca gtgatgatct gaacgggatt
       61 aacgtgaaat accgttatga gtttacggac acgctggggc tggtgacgtc attcagctat
      121 gccaacgctg aagatgagca aaaaacgcat tacagtgata cccgctggca tgaggattcc
      181 gtgcgtaacc gctggttcag cgtgatggcg gggctgtctg tgcgcgtgaa tgaatggttc
      241 agcgcgtatg cgatggcggg cgtggcttac agccgtgtgt cgaccttctc cggggattat
      301 ctccgcgtaa ctgacaacaa ggggaaaacg cacgatgtgc tgaccgggag tgatgacaac
      361 cgccacagca acacgtctct ggcgtggggg gctggcgtgc agtttaaccc gaccgaatcc
      421 gtggccattg accttgctta tgaaggttcc ggcagtggcg actggcgcac tgacggtttc
      481 atcgtgggtg tcggttataa gttctgatta gccaggtaac acagtgttat gacagcccgc
      541 tggttcaggc gggctttttg tggggtgaat atggcagtaa agatttcagg tgtactgaaa
      601 gatggggcgg gtaaacctgt cgtaaattgt gcgattgaac tgcgggccag aagaaccagt
      661 ccgaccgttg tggcacacgt tgttgccact tgcgtgacgg acaataacgg tgcttatgtg
      721 attgaggctg agccggggta ttacgaggtt gcgcttcact gtaacggctg gcagccaacc
      781 cgtgtcgggg atattgatgt ggcaccgact gatgcaccgg ggacactgaa cgcgtttctg
      841 aatgcaccaa aggatggtga tttacgtccg gaggtgatga agcgctttga ggaaatggtg
      901 gcgcaggcgc agcagagtgc cggggctgca gccggaaacg cacagcagac ggcgcaggat
      961 gtggcggcag ccgcaacggc ccgtgatgat gcacaacgtt ttgcggagaa agcccgacag
     1021 gatgcaaccg tcacagctga ggacagaaag gccactgcgg aagatgtgac aagcacagga
     1081 gcaaatgcag ccgcagccgg acagagcgca caggatgccg caggttatgc ccgcgcagca
     1141 gaacaggcca aaaatgacat tgatgctgcg ctgaccggca ctctgaaaat ggctaaccat
     1201 ctgtcagaaa
//
LOCUS       DAZXUL010000355         1183 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZXUL010000355 DAZXUL010000000
VERSION     DAZXUL010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.363"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1183
                     /locus_tag="ACH3L1_004683"
                     /pseudo
     CDS             1..1183
                     /locus_tag="ACH3L1_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          349 a          248 c          290 g          296 t
ORIGIN      
        1 ccttgaagtc gtgaatcact acttcactac tgatgatggt tacaggatca tctcggcacg
       61 ttttggtgtc ccccgaaccc aggttaggac atgggttgcc ctctatgaaa aacatggaga
      121 aaaaggttta attcccaaac ctaaaggcgt tagtgctgat ccagagttgc gtattaaggt
      181 cgtgaaagct gtgatcgagc agcacatgtc ccttaatcag gctgctgctc actttatgct
      241 tgctggtagt ggttctgtag ccaggtggct gaaggtctat gaagagcgcg gagaagctgg
      301 tttacgcgcg ctcaagatta gcaccaaaag aaacattgca atatcagttg atccagaaaa
      361 agcggcatca gcattggagc tgtcaaaaga ccgacgcatt gaggatcttg aaaggcaagt
      421 tcgatttctt gaaacgcggc ttatgtatct aaaaaagctg aaagccttag ctcatcccac
      481 gaaaaagtga aagtactcaa cgagctaagg cagttttatc ctcttgatga gcttctcagg
      541 gctgcggaga taccgcgcag tacgttttat taccatctaa aggctctcag caagcctgac
      601 aagtatgcgg acgttaaaaa gcgtattggt gagatttatc acgagaatag aggccgatac
      661 ggataccgta gggtaacgct gtctcttcat cgagaaggga aacagattaa ccataaagct
      721 gttcagcgcc tgatgggaac cctctcactt aaagcagcga ttaaggtcaa gcgataccgc
      781 tcttacagag gagaggtagg gcaaaccgcc cctaatgttc tccaaagaga tttcaaggct
      841 acgcggccaa acgagaagtg ggttaccgat gttactgaat ttgcagtcaa tgggcgcaag
      901 ctgtatttgt ctccagtaat agatctcttc aacaacgaag ttatttctta cagcctttcg
      961 gaaagaccag tgatgaacat ggttgagaat atgctcgatc aggcattcaa aaagcttaat
     1021 cctcacgagc atcctgttct gcactctgac cagggatggc agtatcgtat gagaagatat
     1081 caaaatatcc ttaaagaaca tggtattaaa caaagcatgt ccagaaaagg caattgtctg
     1141 gataatgctg tggtggagtg tttctttgga accttaaagt cgg
//
LOCUS       DAZXUL010000356         1160 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZXUL010000356 DAZXUL010000000
VERSION     DAZXUL010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.364"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            116..>1160
                     /gene="cadB"
                     /locus_tag="ACH3L1_004684"
     CDS             116..>1160
                     /gene="cadB"
                     /locus_tag="ACH3L1_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFT4079076.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          256 c          298 g          360 t
ORIGIN      
        1 ctttaggatt aatccttttt tcgtgagtaa tcttatcgcc agtttggtct ggtcaggaaa
       61 tagttataca tcatgacccg gactccaaat tcaaaaatga aattaggaga agagcatgag
      121 ttctgccaag aagatcgggc tatttgcctg taccggtgtt gttgccggta atatgatggg
      181 gagcggtatt gcattattac ctgcgaacct agcaagtatc ggtggtattg ctatctgggg
      241 ttggattatc tctattattg gtgcaatgtc gctggcgtat gtatatgccc gactggcaac
      301 aaaaaacccg caacaaggtg gcccaattgc ttatgccgga gaaatttccc ctgcatttgg
      361 ttttcagaca ggtgttcttt attaccatgc taactggatt ggtaacctgg cgattggtat
      421 taccgctgta tcttatcttt ccaccttctt cccagtatta aatgatcctg ttccggcggg
      481 tatcgcctgt attgctatcg tctgggtatt tacctttgta aatatgctcg gcggtacctg
      541 ggtaagccgt ttaaccacta ttggtctggt gctggttctt attcctgtgg tgatgactgc
      601 tattgttggc tggcattggt ttgatgcggc aacttatgca gctaactgga atactgcgga
      661 taccactgat ggtcatgcga tcattaaaag tattctgctc tgcctgtggg ccttcgtggg
      721 tgttgaatcc gcagcagtaa gtactggtat ggttaaaaac ccgaaacgta ccgttccgct
      781 ggcaaccatg ctgggtactg gtttagcagg tattgtttat atcgctgcga ctcaggtgct
      841 ttccggtatg tatccgtctt ctgtaatggc ggcttccggt gctccgtttg caatcagtgc
      901 ttcaactatc ctcggtaact gggctgcgcc gctggtttct gcattcaccg cctttgcgtg
      961 cctgacttct ctgggctcct ggatgatgtt ggtaggccag gcaggtgtac gtgccgctaa
     1021 cgacggtaac ttcccgaaag tttatggtga agtcgacagc aacggtattc cgaaaaaagg
     1081 tctgctgctg gctgcagtga aaatgactgc cctgatgatc ctcatcactc tgatgaactc
     1141 tgccggtggt aaagcatctg
//
LOCUS       DAZXUL010000357         1140 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZXUL010000357 DAZXUL010000000
VERSION     DAZXUL010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.365"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..730
                     /locus_tag="ACH3L1_004685"
     CDS             <1..730
                     /locus_tag="ACH3L1_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFT4079077.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="ACH3L1_004686"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="ACH3L1_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DAZXUL010000358         1133 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZXUL010000358 DAZXUL010000000
VERSION     DAZXUL010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.366"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            21..>1133
                     /locus_tag="ACH3L1_004687"
     CDS             21..>1133
                     /locus_tag="ACH3L1_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HFT4079078.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTD"
BASE COUNT          383 a          148 c          232 g          370 t
ORIGIN      
        1 aaattttata gacgctatat atgggtagta atatacatgg aattagttgc actgcaaata
       61 attatttgaa acaggcctgg aacgatataa aaaatgagta cgaaaaaaat caaacatatt
      121 caatcacgct ttttgaaaac acactggtgt gttttatgcg gttgtacaat gaactcagac
      181 gtaaagtaaa tgaagaggat actccatgtc tggaatgtga atcactagaa aaagaatttg
      241 aggaaatgca gaatgataat gatctatcat tatttatgag aacattgcgt actaatgata
      301 cacaaattta ttcaggggtt tcaggaggta ttacatatac tatacaatat gttcaagatg
      361 ttgatattgt tagagtgtcc ttgccgggca gaggttcaga gtctatcaca gattttaaag
      421 gttattattg gtatggcttt atggaatata ttgaaaacat taatgcgtgt gatgatgttt
      481 tttctgagta ttgtttagat aatgaaaata tgagtatcca gccagaacag ataaatatgc
      541 cgggaatatc tgatttagat actggtattg atttgtctgg catatctttt attcagagtg
      601 aaattaataa aacatatgga ttaaaatacg ctccagtaga tggcgatgga tattgtctgt
      661 taagagccat actggtttta aaagaacatg agtattcatg ggcgctgggc agccataaga
      721 cgcaaaagca agtatacgaa gagtttatta aaattgttga taaacaaacg atcgaggctc
      781 ttgttgatac ggcattctat aatctcaggg aagatgtaaa gacattattt ggcgttgatc
      841 tgcaatctga caacaaaatt cagggtgtgg gtagttttat gtcatggagc tttctgtttt
      901 ttaaaaaaca attcattgat agttgcttga atgacaaaaa atgcatactg catttacccg
      961 agtttatatt taatgataac aagaatttgc ttgctttaga taccgacacg tcggatagga
     1021 ttaaagcggt gaagaatttt cttgctgttc tttcagatag catttgctca ttatttattg
     1081 ttaatagtaa tgtggcatca atctccttgg gaaatgaatc cttttcaaca gat
//
LOCUS       DAZXUL010000359         1110 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZXUL010000359 DAZXUL010000000
VERSION     DAZXUL010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.367"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1019
                     /locus_tag="ACH3L1_004688"
                     /pseudo
     CDS             1..1019
                     /locus_tag="ACH3L1_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1110
                     /locus_tag="ACH3L1_004689"
     CDS             992..>1110
                     /locus_tag="ACH3L1_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079079.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          312 a          271 c          259 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag
//
LOCUS       DAZXUL010000360         1095 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZXUL010000360 DAZXUL010000000
VERSION     DAZXUL010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.368"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(24..827)
                     /gene="dkgB"
                     /locus_tag="ACH3L1_004690"
     CDS             complement(24..827)
                     /gene="dkgB"
                     /locus_tag="ACH3L1_004690"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFT4079080.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(990..1066)
                     /locus_tag="ACH3L1_004691"
     tRNA            complement(990..1066)
                     /locus_tag="ACH3L1_004691"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1030..1032),aa:Asp,seq:gtc)
BASE COUNT          285 a          258 c          249 g          303 t
ORIGIN      
        1 cccggaggag ctgtcagaga ggcttaatcc cattcaggag ccagaccttc cgggctaacc
       61 aggcggtcgt tgcaatccag tgcggcgatc gcttttttat cttcggcatc aagctgtaaa
      121 ttttgtgcct taagattact ttccaggttt ttacgtttag tagaagaagg aattactgag
      181 taaccttccc ccatagccca cgccagaatc acttgtgccg gaatcgcatt gtgtttagct
      241 gcaatacgag caataacctc atctttcagt gccttaccat aagccagcgt catataggaa
      301 gtgatatgaa taccgtgctg tttagcccag gcaaccactt tacggttttg cagataagga
      361 gagagttcaa tctggttggt agcgatgttt tcagcgccaa cagcagcaat cactttttcc
      421 atcaatggga tcgtgaagtt ggaaataccg atctcacgcg tcagcccttg ttttttggct
      481 tccagcagcg cctgcataaa ctcttcaaca gagacttcat cgtttggtga cggccagtgg
      541 attagcgtca gatcaacata atcggtacgc aatttttgca ggctctcttt cagactcggg
      601 atcaatttgt ctttgctgag attttcaatc cagattttag tggtgatgta gagttcatga
      661 cgtggcacgc cactttctgc aatcgcctga cctactgcgg cttcgttatc atagatttgt
      721 gcggtatcaa ttgcgcgata accaagttca agcgccgttt tcacagatga aataacaacg
      781 tcgtctttca gacggaaagt acctaaacca aatgcaggga tagccataat atgcctcttt
      841 taatgctatt ttcgttaact gaggtgatta tgggcttcag actgttgcgg gaaaagagcg
      901 taaaacgcag aggatttttg cgattctagc aataatagat atccagacgc aaaaaaacat
      961 cgagcgttaa ctcgaggctt cttaataagt ggcggaacgg acgggactcg aacccgcgac
     1021 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1081 caaccaccgg tttta
//
LOCUS       DAZXUL010000361         1094 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZXUL010000361 DAZXUL010000000
VERSION     DAZXUL010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.369"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            296..>1094
                     /locus_tag="ACH3L1_004692"
     CDS             296..>1094
                     /locus_tag="ACH3L1_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFT4079081.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLSETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGP"
BASE COUNT          355 a          238 c          211 g          290 t
ORIGIN      
        1 gagcaggtac cggcatccac gttacagacg gtcccggcaa aagtgcctga cctgacccct
       61 attagcggtt accagcgccc caccagaccc ggtgacatca ccccccggat catggacgac
      121 tgggggcagt aatcatgccg ggtctggctg gctaacaccc cattataagg ggtacgaata
      181 aagtaacggg ggaagcaata cctggagaaa gagtacaaag caaactgtat taaacttaat
      241 aagttatatt gtatttcact agagttctta tcgttgataa gaaattctgg ttataatgat
      301 taaatcaacg aatatacagg taatcggttc tggtattatg catcaaataa acaacataca
      361 ctcgttaact ctattttctt tgcctgtgag tttgagtcca tcttgtaatg aatattattt
      421 aaaagtctgg agtgaatggg aaaagaacgg taccccaggc gagcaacgca atatcgcctt
      481 caataggcta aaaatatgtt tgcaaaatca aggggcggaa ttaaatctat ctgagttaga
      541 tttaaaaaca ttacctgatt taccgcctca gataacaaca ctggaaataa gaaaaaacct
      601 attaacatat ctccctgatt tcccaccaat gcttaaggta atacatgctc aatttaatca
      661 actggaaagc ttacctgcct tacccgagac gttagaagag cttaatgtgg gtgataacaa
      721 gataaaagaa ttaccatatc tttctgaaac tctaactcat ttacggatcc ataataaccg
      781 attgcatatt ctgccactat tgccaccgga gctaaaatta ctgatagttt ctggaaacag
      841 attagacagc atccccccct ttccagataa gcttgaaggg ctggctctgg ctaataattt
      901 tatagaacaa ctaccggaat taccttttag tatgaacagg gctgtgctaa tgaataataa
      961 tctgacaaca cttccggaaa gtgtcctgag attagctcag aatgccttcg taaatgttgc
     1021 aggtaatcca ttgtctggcc ataccatgcg tacactacaa caaataacca ccggaccaga
     1081 ttattctggt cctc
//
LOCUS       DAZXUL010000362         1071 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZXUL010000362 DAZXUL010000000
VERSION     DAZXUL010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.371"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..563
                     /locus_tag="ACH3L1_004693"
     CDS             <1..563
                     /locus_tag="ACH3L1_004693"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFT4079082.1"
                     /translation="NTKGDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTD
                     NYQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAK
                     SGLTGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVM
                     VNEVETALFNARALALKIYSMSGWNL"
     gene            790..>1071
                     /locus_tag="ACH3L1_004694"
     CDS             790..>1071
                     /locus_tag="ACH3L1_004694"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HFT4079083.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          316 a          202 c          257 g          296 t
ORIGIN      
        1 ataacaccaa aggcgatgcc atgattggcg tcaagcagcc tttaccaaaa gcagttttaa
       61 gaactcagca tgacaaaaat aaagaagcaa taagtatcct ggattttggt gttattgatg
      121 atggtgtgac agataattac caggcaatac aaaatgctat agatgccgtt gcttcactac
      181 cctccggcgg ggagctgttt atccctgcga gcaaccaagc ggtggggtat attgttggat
      241 ccactttgct tattcctggc ggtgttaaca tcagaggagt tggtaaggca tcgcaactcc
      301 gagcaaaaag cggacttaca ggatctgtgt taaggctgtc ttatgattca gacactatcg
      361 gccgttatct gagaaatata cgagtaactg gtaataacac ctgcaatggt attgacacaa
      421 acattacagc agaagactct gtcatcagac aggtttatgg ctgggtattt gataatgtaa
      481 tggtgaatga agttgaaacc gcattattta atgcaagggc tctggcactc aaaatttata
      541 gcatgtcagg ctggaacctg tagagtcggt cttcactttt taggccagtg cgtaagtgtt
      601 agtgtcagct cctgccattt cagcagagga aattattctg ctgatgaaag ctttggcatc
      661 aggattcagc ctcaaacgta tgcgtggtcg tcagaggcag ttaggtcaga agcaataatt
      721 ttagacagtg agaccatgtg cattggtttt aaaaatgccg tctatgttca tgattgcctt
      781 gatttgcata tggaacaact ggatttagat tattgcggct caacaggcgt ggttatagag
      841 aatgtaaacg gaggattttc tttctcaaac tcatggatag cagcagatgc cgatggcact
      901 gaacaattta cggggatata ttttagaacg ccggcctcaa cgcagtcaca taaaattgtc
      961 agtggtgttc atatcaacac tgcaaacaaa aacacggctg caaacaatca aagtatagcg
     1021 atagaacagt cggcgatctt cgtctttgta agtggttgta cgttaactgg t
//
LOCUS       DAZXUL010000363         1070 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZXUL010000363 DAZXUL010000000
VERSION     DAZXUL010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.372"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(228..653)
                     /locus_tag="ACH3L1_004695"
     CDS             complement(228..653)
                     /locus_tag="ACH3L1_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFT4079084.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(650..1033)
                     /locus_tag="ACH3L1_004696"
     CDS             complement(650..1033)
                     /locus_tag="ACH3L1_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFT4079085.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          308 c          265 g          244 t
ORIGIN      
        1 tctcgcgtat ggttgattga caccacagtg cgaattcggc gaatgcgaga tcacggtacg
       61 attccgcacc acactaccaa tcaatacccc gggttttatt caggtaagca ggactgcgga
      121 tatccggtgt cgcgcctttg tgtcacgaac ggggtgggtg cgaaacacca gataaaatac
      181 aggttggcaa tacctctgaa cgccctgagc aggtcgggca tttcggatta agtactcgca
      241 cctccgcagt cctcaaacaa gtctggctgg tagctgtaaa cagacttcgt acataccgct
      301 ctgcaataaa tccccgtacc acaggcctca cagaacttct tcccatgaaa aaactgctca
      361 cacatcacac aatgccactc cagcacgacc ggtaatggcg ataaaaacat cgccatatcc
      421 tcaatgtaag ggtgggactt ctccggattc agcaccacac agtccgcctt ctgttgctca
      481 ctcagggcat gtaaatcatg ctcaaaccac gccccctgag catctgtctg cagaatcaac
      541 cggccatggc aggaaaggca gaaacactgc cggatatttc tgctgagcgc agagtcggca
      601 ctgataatgt gctcacccgg cgtgatcccc agccccgttt ttataccgtt cattctgctc
      661 ccccctcctc actgaagcgc ccggtctggc agtgtgtgca gtactgctcc ccataataat
      721 cgtggtgaca ttgcctgcag tgccagctgg ctttacgtac tacgggtaag gtatccggga
      781 cgaattgctg cagacgttta atcaaccgta tttctctgcg ttccggcctg acataagggc
      841 actcatgacc gtgctccgtc agcccttcgt cagcgtgttc aaaccagggc cgttcagtgt
      901 cgtattgcgg gtggtatctg agtgcactgc cgcaaaggtg gcaggtgtag ctatcgtaag
      961 gtgcagtctg tgcggtacgg gcaccggtca gacgtccgtt gccatcaagt gcgagaaaag
     1021 attttgcgta catagtatat gttcctcagc cgccagacga cacgcaggca
//
LOCUS       DAZXUL010000364         1066 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZXUL010000364 DAZXUL010000000
VERSION     DAZXUL010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.373"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(570..749)
                     /locus_tag="ACH3L1_004697"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="ACH3L1_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="ACH3L1_004698"
     CDS             843..>1066
                     /locus_tag="ACH3L1_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4079086.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAZXUL010000365         1065 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZXUL010000365 DAZXUL010000000
VERSION     DAZXUL010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.374"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACH3L1_004699"
     CDS             complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACH3L1_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFT4079087.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          300 a          322 c          248 g          195 t
ORIGIN      
        1 tttaatccta tctgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa
       61 gccactggcg gagggagcgc gcagggggca gccagtcgcc gccgccccct gcacccccgg
      121 gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggcaaccgg
      181 gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat gtgggctgcc
      241 ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc ggtttgtaat
      301 gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatca cgcttttcca
      361 gcagagtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
      421 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
      481 ggttaacaga agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac
      541 caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg tggatcacta
      601 acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
      661 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
      721 ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg
      781 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
      841 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc atcgtcagaa
      901 cggtcaaacc aaatgccaac gccacgccgg caaaaccaat gcctaattcc gggaagcctg
      961 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
     1021 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accct
//
LOCUS       DAZXUL010000366         1038 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZXUL010000366 DAZXUL010000000
VERSION     DAZXUL010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.375"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..104)
                     /locus_tag="ACH3L1_004700"
     CDS             complement(<1..104)
                     /locus_tag="ACH3L1_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HFT4079088.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="ACH3L1_004701"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="ACH3L1_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFT4079089.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
BASE COUNT          287 a          241 c          183 g          327 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaactgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcctg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tattttttat
     1021 atgtattatt agtaagtt
//
LOCUS       DAZXUL010000367         1021 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZXUL010000367 DAZXUL010000000
VERSION     DAZXUL010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.376"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(50..190)
                     /gene="yqcG"
                     /locus_tag="ACH3L1_004702"
     CDS             complement(50..190)
                     /gene="yqcG"
                     /locus_tag="ACH3L1_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFT4079090.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="ACH3L1_004703"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="ACH3L1_004703"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFT4079091.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          205 c          237 g          283 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt tcccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtta
     1021 t
//
LOCUS       DAZXUL010000368         1014 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZXUL010000368 DAZXUL010000000
VERSION     DAZXUL010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.377"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>1014
                     /gene="gadC"
                     /locus_tag="ACH3L1_004704"
     CDS             <1..>1014
                     /gene="gadC"
                     /locus_tag="ACH3L1_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFT4079092.1"
                     /translation="VAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIP
                     YFPTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQV
                     SKWCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFL
                     AYAGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQS
                     LLVAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQ
                     KTNRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASA
                     IYLR"
BASE COUNT          240 a          184 c          234 g          356 t
ORIGIN      
        1 gttgcctttg ttgcggctga gctagctaca acatatccac aaaaaggggg ggcatttcga
       61 tggataggag aagcatttgg ggctcgctgg ggttttgtag gaatgcttat gacctggcta
      121 caagtcatcc cctactttcc aacagtactg acatttggtg cagtctcggt tgcatttatt
      181 gatgttgata tgggggtggc agaatcaatt gccagtaata aatggtatat tttatttttc
      241 gtactatttg tttactgggg agctatttgt gttgcattac gtggcgttgg tattttctcg
      301 caggtatcta agtggtgcgg tattataggg acaattattc cagctattat ccttgttatt
      361 cttggtttct cctatctgtg gttcagcggt aaaccagcgg tgatcccatt aggttggggt
      421 gatcttttcc ctaatatagc taacttccag aatattgttc ttgccgccag catatttctt
      481 gcttatgcag ggatggaaat gaatgcggtc catgttaacg atttagataa tccaactaaa
      541 aaatacccaa ttgcaattac catcgcttct ataggtacta ttgcaatatt cctgctcagt
      601 actttagggg tcgcctttat tattcctaca gataaaatta gtctaactca aagtttgttg
      661 gttgcctacg atatgctgtt cgaatgggct ggagtgccat ggcttggctc tgtgatggca
      721 tttatgctag caattggcgt actgggtggg gtggttactt ggattgcagg gcctaataca
      781 ggtgtattag cgatcgcgaa agctggttat ttacctaagt ttttccaaaa aaccaatcgt
      841 catggaatgg ggcaccatct aatgtttgta caaggtataa ttgtctcagt tctttctgtt
      901 acttttgtga taatgccttc agttcaagca gcttttcaga ttttatcaca gttaacagtt
      961 atgctctatt tagttatgta tatgctcatg ttcgctagtg ctatctatct acga
//
LOCUS       DAZXUL010000369          995 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZXUL010000369 DAZXUL010000000
VERSION     DAZXUL010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.378"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..887)
                     /locus_tag="ACH3L1_004705"
                     /pseudo
     CDS             complement(1..887)
                     /locus_tag="ACH3L1_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            895..>995
                     /locus_tag="ACH3L1_004706"
     CDS             895..>995
                     /locus_tag="ACH3L1_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFT4079093.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRR"
BASE COUNT          218 a          249 c          241 g          287 t
ORIGIN      
        1 tttaaacctg attttgcccg gacattgcgc cccggctggc tgaccgtacc cgctgctgac
       61 tttgacatgt tgctgacctt caaatcctca atgactatca ttgcgtggtt tttgctgacg
      121 gccgttgtga ctttgtgaag gtagtccctg cggatatttg cgatacagga atgcagtcgc
      181 tgtattttgc gtttctgctt ctgccagttg ttgctgaatt tgaccttgcg gcttaactgt
      241 cgctgaagtc tcgccagcgt cttctggttt ttctgaaaac tgtttacagg ctcaaagact
      301 gtgccatctg acagcgtggc gagtttagcc acgccagcat ccagcccgac cattgatgct
      361 gaagggtgag ccggagtgga tacttcactt tctgtctgaa tactgatgta ccacgtaccg
      421 caggactggc tgacagtgac atttttcaca acacccgtga cctgacggct attccggtag
      481 cgcatccagc ccagtttcgg cagaaaaata cggctgtttt cctgatcgag cttaacgccc
      541 tgcgggtagc ggaatgcatc attctgtcac cgctttttga atcggggaaa agcagcccgc
      601 ttccggaaga agtttttgta agcccgctca aggtctttca gtgactgttg caatggctgt
      661 gagggagaat ctttaagcca ttgcgtttca gtggcatttt tccactcaac cagccaggaa
      721 gccattttcc cgtaagggat gtatttatta ccggccttat gattctcatt ctgacgtgcc
      781 agagcacgat tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc acactcctgt
      841 tgaccacctg gtcttaactg gaatttaaat gcctgtaatc gtttcacagg tcaaatgata
      901 ctttggtctg tgaaaaaaga aaccgatatc cggcgtggaa ggcattgtgt tttcctgatg
      961 catgttcacc tagtctttgt caccagatac cgacg
//
LOCUS       DAZXUL010000370          936 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZXUL010000370 DAZXUL010000000
VERSION     DAZXUL010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.379"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            144..641
                     /locus_tag="ACH3L1_004707"
                     /pseudo
     CDS             144..641
                     /locus_tag="ACH3L1_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            682..873
                     /locus_tag="ACH3L1_004708"
                     /pseudo
     CDS             682..873
                     /locus_tag="ACH3L1_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          244 a          232 c          269 g          191 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcaaagcgcc agccccgtcc cgcccgatag agcgcggcat
      121 agcgggcccc ggattacttg cgtgcgacgg cagaagaagc cctgaagcga atcagtgaac
      181 tgtacgccat cgaggatgaa atacggggat taccagagtc agagtgtctt gcagtcaggc
      241 agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag aagaatggca
      301 cgctgtcgaa aaaatccaga ctgggcgaag cattcagcta tgtactgaat cagtgggacg
      361 ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca gcggaaagag
      421 cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc gatcacggcg
      481 gcgagcgtgg tgcactgctg tacgggctga tcggcacctg ccgtctgaac ggtatcgatc
      541 cggaagcgta tctgcgccat attctgagcg tactgccgga atggccttcc aaccgagttg
      601 acgacctcct gccatggaag gtggtgctac catcaggata atcgtcaata cggtgttcgg
      661 tgaacgttta cttggagcat catgcttcat gaagaaaaac tggcacgtca tcaacgtaaa
      721 caggcgatgt atacccgaat ggcagccttc ccggcggtga aaatgttcga agagtatgac
      781 ttcacgttcg ccaccggagc accgcagaag cagctccagt cgttacgctc actcagcttc
      841 atagagcgca atgaaaacat agtcttactg gggacatctg acatcacaaa tccccgggtg
      901 gggatttgtg tataagagac agcccatgga ggaagg
//
LOCUS       DAZXUL010000371          932 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZXUL010000371 DAZXUL010000000
VERSION     DAZXUL010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.380"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(55..243)
                     /locus_tag="ACH3L1_004709"
     CDS             complement(55..243)
                     /locus_tag="ACH3L1_004709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079094.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            complement(540..778)
                     /locus_tag="ACH3L1_004710"
                     /pseudo
     CDS             complement(540..778)
                     /locus_tag="ACH3L1_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          245 a          181 c          216 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aa
//
LOCUS       DAZXUL010000372          926 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZXUL010000372 DAZXUL010000000
VERSION     DAZXUL010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.381"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(273..755)
                     /gene="sseA"
                     /locus_tag="ACH3L1_004711"
                     /pseudo
     CDS             complement(273..755)
                     /gene="sseA"
                     /locus_tag="ACH3L1_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT          219 a          248 c          220 g          239 t
ORIGIN      
        1 gcaggatttg tttatatttg tctgaattcg gactaataag ccaggttatt ttattaccga
       61 acacggcgga aagggccggt gtcgataata cgctaaataa acaataatta ctctcttttg
      121 cttgacaaaa agagagttac tggtgagtat tgttttgctg tgaagcacct gcgttgctca
      181 tgtaacacaa gtcaacctgc tggaaaggcc gcgaaccaga ccagcaataa aaaaacgcca
      241 aattcggcgg ttttttattg cctgtaatat acttatttca ctggctcaac cggtaaatct
      301 gcccgcgcgc cccattcact ccatgcgccg tcgtacagtt tcacgtttgg cacatccagc
      361 gtcgcgagtg ctaacaaaac cacggctgcc gttacaccag agccgcagct gacgataatc
      421 ggtttgtcat agctgacgcc gcgaccaaaa aatatcgcat ccagttcatc ggtcgttttt
      481 agttcgcctt cgcgcaccag ttccgtccac ggaacgttca atgcaccggg aatatgtccg
      541 cgacgtaaac ctgggcgagg ttcatcaact tctgcgttaa aacgtgcagc cgggcgggca
      601 tcaataattt gcgccgtatt ttcatggctt gccaataaca catcggttac tttcaccact
      661 gcttcaggat taaacgcggc gttaaactct ccttccggca gctctactgc accttcttct
      721 aatagcaggt catcgcgctg ccagcctgca agtccccgtt actttattcg taccccttat
      781 aatggggtgt tagccagcca gacccggcat gattactgcc cccagtcgtc catgatccgg
      841 ggggggatgt caccgggtct ggtggggcgc tggtaaccgc taataggggt caggtcaggc
      901 acttttgccg ggaccgtctg taacgt
//
LOCUS       DAZXUL010000373          923 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZXUL010000373 DAZXUL010000000
VERSION     DAZXUL010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.382"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..200
                     /locus_tag="ACH3L1_004712"
                     /pseudo
     CDS             1..200
                     /locus_tag="ACH3L1_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..>923
                     /locus_tag="ACH3L1_004713"
     CDS             300..>923
                     /locus_tag="ACH3L1_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFT4079095.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          293 a          217 c          205 g          208 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatctgaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aag
//
LOCUS       DAZXUL010000374          907 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZXUL010000374 DAZXUL010000000
VERSION     DAZXUL010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.383"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            487..>907
                     /gene="ospC4"
                     /locus_tag="ACH3L1_004714"
     CDS             487..>907
                     /gene="ospC4"
                     /locus_tag="ACH3L1_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HFT4079096.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSLEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFN"
BASE COUNT          315 a          170 c          170 g          252 t
ORIGIN      
        1 atcccgtact ctctggccag ttgggccacg caggccccta gctggtaaga tttttcaata
       61 agatcaagct taaactccat agagtcatag agtaagaggc acgaatcctg cgggggcgaa
      121 gagaatctga cattgataga gtccattaac gttataagag gactctatca tcgccaatca
      181 tcagacattg aaatagacga cactgagatg acgcttacaa ttcttttggt ttctgtaata
      241 agtaatctga catcccccgt gcccagaaga ggagagggat atcaagccca agggttaata
      301 attgatacta cggttaaaat tgccttcagt aaaggaaacg tgattaaatt caggggattt
      361 cagagtgaaa accatacaac atattaaaat ctgttcattt agctataata catacaagaa
      421 agtgtccagt tagataatgt tatctaaata accacagata aaaacgcaca taattggggg
      481 ggggggatga aaatacctga agcagtaaat catattaatg ttcagaataa tattgatctt
      541 gttgatggaa aaacgaatcc gaataaagca acaaaagcat tacaaaaaaa tgtattacgc
      601 gtaacaaact catcttcttc tggcataagt gaaaaacatt tagatcactg tgctaatact
      661 gtgaaaaact ttttaaggaa gtctatagca gcgcaaagtt atagcaaaat gttttctcaa
      721 gggaccagtt tcaaatcact aaatctttct cttgaagcgc catctggagc acggtcatca
      781 tttcggtcac ttgagcatct cgataaggtg tccaggcact atatctctga gataatacaa
      841 aaagttcatc ctctttcttc agacgaacgt cacttacttt ctattattat taattctaat
      901 tttaact
//
LOCUS       DAZXUL010000375          893 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZXUL010000375 DAZXUL010000000
VERSION     DAZXUL010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.384"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..143
                     /locus_tag="ACH3L1_004715"
     CDS             <1..143
                     /locus_tag="ACH3L1_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFT4079097.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="ACH3L1_004716"
     CDS             127..486
                     /locus_tag="ACH3L1_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079098.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /gene="yfdT"
                     /locus_tag="ACH3L1_004717"
     CDS             486..791
                     /gene="yfdT"
                     /locus_tag="ACH3L1_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFT4079099.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            707..>893
                     /locus_tag="ACH3L1_004718"
     CDS             707..>893
                     /locus_tag="ACH3L1_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4079100.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQAT"
BASE COUNT          280 a          194 c          227 g          192 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cac
//
LOCUS       DAZXUL010000376          887 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZXUL010000376 DAZXUL010000000
VERSION     DAZXUL010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.385"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            270..743
                     /locus_tag="ACH3L1_004719"
     CDS             270..743
                     /locus_tag="ACH3L1_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4079101.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          270 a          186 c          197 g          234 t
ORIGIN      
        1 aatcgctgga ttcggtcatt ctgtctccct tgtatatggg ttaaccagat aaccacgggg
       61 agcaggtacc ggcatccacg ttacagacgg tcccggcaaa agtgcctgac ctgaccccta
      121 ttagcggtta ccagcgcccc accagacccg gtgacatcac cccccggatc atggacgact
      181 gggggcagta atcatgccgg gtctggctgg ctaacacccc attataaggg gtacgaataa
      241 agtaacgggg gggagatgaa ccctatgcaa tgtcctggtt aatatctcag tgtgctcatc
      301 agtgcactga taataaaaag acggagacag atgccattta cgataaagtt cggagttctt
      361 atttgctttc atgtattctg aaaaaaaaca aaaatgttgg gcttattctt catgcaccat
      421 catttgtttc tgtgtctgaa aaaatcgcca gaattgtgat ggcaaattac tcccgtaact
      481 ggagtaattc cgagttggcc agtgcggttt taatgagtga atcttcttta aaaagaagaa
      541 tgtacaaaga ggtcggttct ataagtacat ttgtacataa aataaagctt acagaagcta
      601 tcaggaaatt acgaaggaca aatactccca tttctgtcat atcatctgag ttaggttaca
      661 gttcaccatc gtatttttct aaagtcttct ttaaatattt aaagacatac cctcaaaaca
      721 taaggaagaa aaatggtcgg taatgtcacc aattaggagg acttgcagag ccgctttttt
      781 acgatactga gatcaaacag acataatcta tgcggagatt tacagcaggg catggttcac
      841 aaatctgaca gcgatgaatt atcggcactg cgggcagaaa atgctcg
//
LOCUS       DAZXUL010000377          870 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZXUL010000377 DAZXUL010000000
VERSION     DAZXUL010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.386"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>870
                     /locus_tag="ACH3L1_004720"
     CDS             <1..>870
                     /locus_tag="ACH3L1_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFT4079102.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAZXUL010000378          851 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZXUL010000378 DAZXUL010000000
VERSION     DAZXUL010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.387"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..162
                     /locus_tag="ACH3L1_004721"
     CDS             <1..162
                     /locus_tag="ACH3L1_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFT4079103.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
     gene            complement(270..569)
                     /gene="pliG"
                     /locus_tag="ACH3L1_004722"
                     /pseudo
     CDS             complement(270..569)
                     /gene="pliG"
                     /locus_tag="ACH3L1_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
     gene            633..>851
                     /locus_tag="ACH3L1_004723"
     CDS             633..>851
                     /locus_tag="ACH3L1_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFT4079104.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARL"
BASE COUNT          232 a          178 c          196 g          245 t
ORIGIN      
        1 ttacaaggct acgatcccag cgaatttaca ttcgctaacg gtgtattttg cgatgttaaa
       61 gaaaaattat gtcgtgatga tcgttatttt ggtgtagatg gtaagcggag tggaaaaatc
      121 aatcaaacca ccacaaagat gttatttatg tgtcgtgagt gatgccttgt ttacattatg
      181 ttctctgcta ttcgtcatta acctcttgag cgacagagag gagtggggcg ttaacgtcta
      241 tgacggggat agtccccgtc ggttgacatt tacttaattt gaatatcgac gttatatttt
      301 ttagttttgt ttttacgggc gtcgtttcga gtttgcagca ctcgcagctc atatttgcca
      361 gaagcaggga gtgagtattg tccgtgacta tcgagttctg gggaatatct ggataaatca
      421 acagaatcat cgattcctgg cccgaagaga taagtatcag cgccttcatt agagatactc
      481 acatgcactt tctgaccttt tttggcatag aaggtatatg tatcgtaatc gtatcccttt
      541 atttcgccgg agtactgagc gctgctatga tcttacccag caatagtgga cacgcggcta
      601 agtgagtaaa ctctcagtca gaggtgactt acatgacaaa aacagtatca accagtaaaa
      661 aaccccgtaa acagcattcg cctgaatttc gcagtgaagc cctgaagctt gctgaacgca
      721 tcggtgttac tgccgcagcc cgtgaactca gcctgtatga atcacagctc tacaactggc
      781 gcagtaaaca gcaaaatcag cagacgtctt ctgaacgtga actggagatg tctaccgaga
      841 ttgcacgtct c
//
LOCUS       DAZXUL010000379          842 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZXUL010000379 DAZXUL010000000
VERSION     DAZXUL010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.388"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            59..775
                     /locus_tag="ACH3L1_004724"
     CDS             59..775
                     /locus_tag="ACH3L1_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFT4079105.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          284 a          140 c          173 g          245 t
ORIGIN      
        1 ttaacacatg ttaacgattt accagtaatg taaataaatt ttcgaggaga tcattccagt
       61 gggacgtaaa tgggccaata ttgttgctaa aaaaacggct aaagacggtg caacgtctaa
      121 aatttatgca aaattcggtg tagaaatcta tgctgctgcc aaacaaggtg aacccgatcc
      181 agaattaaac acatctttaa aattcgttat tgaacgtgca aagcaggcac aagttccaaa
      241 gcacgttatt gataaagcaa ttgataaagc caaaggcggc ggagatgaaa cgttcgtaca
      301 gggacgttat gaaggctttg gtcctaatgg ctcaatgatt attgctgaga cattgacttc
      361 aaatgttaac cgtacgattg ctaacgttcg cacaattttc aataaaaaag gcggcaatat
      421 cggagcggca ggttctgtca gctatatgtt tgacaatacg ggtgtgattg tatttaaagg
      481 aacagaccct gaccatattt ttgaaatttt gcttgaagct gaagttgatg ttcgtgatgt
      541 aactgaagaa gaaggtaata ttgttattta tactgaacct gctgaccttc ataaaggaat
      601 cgcggcgcta aaagcagctg gaatcactga gttctcaaca acagaattag aaatgattgc
      661 tcaatctgaa gttgagcttt ccccagaaga tttagaaatc tttgaagggc ttgttgatgc
      721 ccttgaagat gacgacgatg tacaaaaagt ttatcataac gtcgcaaatc tctaattata
      781 ttttaaagaa atctgtcttt atggcagatt tctttaatct catataattc ttataaaaaa
      841 ta
//
LOCUS       DAZXUL010000380          832 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZXUL010000380 DAZXUL010000000
VERSION     DAZXUL010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.389"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..327
                     /locus_tag="ACH3L1_004725"
     CDS             <1..327
                     /locus_tag="ACH3L1_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFT4079106.1"
                     /translation="VSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNG
                     LLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVI
                     LKITYN"
     gene            complement(370..570)
                     /gene="glgS"
                     /locus_tag="ACH3L1_004726"
     CDS             complement(370..570)
                     /gene="glgS"
                     /locus_tag="ACH3L1_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFT4079107.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          155 g          241 t
ORIGIN      
        1 gttagtgaga cgtttagtgt agtcatgaca aaagatcctg gtgcggcctg tactgagcaa
       61 ttcaatattt tagggagctt tttcactacg gatattttga gtgattatag ccatctggat
      121 atgggtaatg gactgctatt gaagatattt cataaagatg gaacagcaac ggaatttaac
      181 cgcttctcac aatttgcttc tttttcatcg tctagtgcac cttcggtcac cgcaccattc
      241 agggcagaac ttagtgcgaa cccggcagag actgttgttg agggaccgtt tagtaaagac
      301 gtaatcctca aaatcaccta taactagcat ctaatacaaa cactaaaacg ggccatcagg
      361 cccgttatat cagtgctcta actccagctc tcttgcctgc atcatctgtt ggcaatacca
      421 ggcataacgt gcgcaaaacc aagttctatg ttcttcatcc atgttaccag taacagccag
      481 aatatcgacc gggcgacctt ctgcgtgcat tctggcaaaa ctacgcgcca ggaaatcgaa
      541 attattcaaa gaattaagac tatgctccat aagcacatcc tccggttatc gcataccttt
      601 taaggccata tgctttttct gatttcagta ttccccaatc agtgtagtgc ctttaaacat
      661 aacgtgcgta aatatattgt ccccgatcac actttttagt gaaaaataaa aaaccatatt
      721 aaaatacttt ctcttccaga acttctctaa catgtgttat tattaacaaa gacaaatgta
      781 agcagggaaa ctattccaag gtaaatattg cgtcactaaa tggacattgg ag
//
LOCUS       DAZXUL010000381          825 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZXUL010000381 DAZXUL010000000
VERSION     DAZXUL010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.390"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(42..710)
                     /locus_tag="ACH3L1_004727"
                     /pseudo
     CDS             complement(42..710)
                     /locus_tag="ACH3L1_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            complement(700..>825)
                     /locus_tag="ACH3L1_004728"
     CDS             complement(700..>825)
                     /locus_tag="ACH3L1_004728"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFT4079108.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          267 a          213 c          185 g          160 t
ORIGIN      
        1 aaagaacaaa tggtagtgtc cgctattgcc agtacacctc aatatggcag tgctattttg
       61 attcgacgtt gatcaagttt ctgataattc caacgacggt cgacgggcac catccagtca
      121 atcaacatac ctaccgccag ccctgtgatg acatcaataa aatgatgctg ccatgtcgtc
      181 agcgtcgaaa tggcgatgag taaaaaccat ccgccacaga ctttacgcca cctcacagcc
      241 agatgctgac gaaagtgacg ccaaagtagc cagcagagaa taatatgcag cgaaggagac
      301 tggttataag gcagatcaaa cagttcaagt tgcgaaaata gccatcctgt caccccactc
      361 acttcaggac ggataaaact aaacttcagc gggtagagaa aaaaaccgca gcaggccatt
      421 accgttgcca gaataagccg gtggacaagt cggcgttgtt cgaatgtcga gctacaaacg
      481 aacagcgaaa atccatataa aagatccaga ctccagtaag gaacaatagt ccagggaaga
      541 aaagggatcg ccgtttccca accgaatacc tgactgggga tatcatggct gttaaggtcc
      601 tgaaccgcgg tgaactgatt aagagatcca taggtgaaga agaaaaacgg ggccagcaac
      661 aataaccagc cagcgccttg tagcaatacg ttccgacgtt cagttatcac gacgcaccgc
      721 catcgaaacc gtaaaaatgc cccacacatc aatccgttga gtgcatttat caaatccggc
      781 attacgcacg agcgaatcca tctccccttg cgaacgcacg cgcat
//
LOCUS       DAZXUL010000382          819 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZXUL010000382 DAZXUL010000000
VERSION     DAZXUL010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.392"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            10..640
                     /locus_tag="ACH3L1_004729"
                     /pseudo
     CDS             10..640
                     /locus_tag="ACH3L1_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816255.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
BASE COUNT          253 a          133 c          169 g          264 t
ORIGIN      
        1 gcgttcatta tgaaaaataa atcaggtaat tcttgttata gagaagcagt cgatattcat
       61 agctcacttg agctttcatt tatattgcat aaatatcatt tgcgtgtaga tgagaagaaa
      121 tctcgaaatg tattcagcgc catacgtgat gagctcatcc ttgatgggga ttcacgacaa
      181 aacttagcta ctttctgtca aacatggctt gatgatgaaa tccgtgagtt gcttaattta
      241 gctattgata aaaacatgat ttataaagat gaatatccac aaatggcgga acctgaaggt
      301 cgctgtgtgc gtatgctttc cgattcgtgg agtttccccg attcgcgaca caccttaggt
      361 tgctcaacca ttggttcatc tgaagctgct atgctcggcc gcttggcgtt aaaatggcag
      421 tggtgtaaaa aacgcgaagt acaaggtaaa tcaaccgaac ctgatttgcg atcctgtgca
      481 aatatgctgg cataaattct gccgttactg tgatgtgaaa ttgtgtgaaa ttccacttga
      541 tgatcatcac ctgataatga atgctaaaga ggttttgaag catgtcgatg aaaacactat
      601 tggtgtcgtt acgactttag gtattactta tttttgtaaa gcagattgcg ttagtgtttt
      661 taagataagt gttaaagtta atattagttt ttatatttac atcgtaatta tatgtttcta
      721 ttttaaaaat gttcgagagt ataaaaatgt ctaatgctaa tatcgcaccc aaaagtaaga
      781 aaatgacagt ggggacactc gctatcatga atataactg
//
LOCUS       DAZXUL010000383          810 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZXUL010000383 DAZXUL010000000
VERSION     DAZXUL010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.393"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..264
                     /locus_tag="ACH3L1_004730"
     CDS             <1..264
                     /locus_tag="ACH3L1_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFT4079109.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>810
                     /locus_tag="ACH3L1_004731"
     CDS             288..>810
                     /locus_tag="ACH3L1_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFT4079110.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMAG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAA"
BASE COUNT          220 a          188 c          237 g          165 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat attattctga
      361 aatcccgggt aaacaccccg gaagtggtga tgaacactgt tgctgatgtg atggcaggaa
      421 acgatggtga atactgtttc catgcgcgga ctggaaaata tggtgtgtat ctgaaacagg
      481 actggcgcaa cgagtacaac gttggcgaca ttgctgtata tgaggactca aagcccggca
      541 cgctgaatga ctttctgatt gctcctgatg agggcgacct gaaaccggat gtcgtcaaac
      601 gctttgagga aatggtggcg caggcgcagc agagcgccgg ggccgcagcc ggaaacgcac
      661 agcagacggc gcaggatgtg gcggcagccg caacggcccg tgatgatgca caacgttttg
      721 cggagaaagc ccgacaggat gcaaccgtca cagctgagga cagaaaggcc actgcggaag
      781 atgtgacaag cacaggagca aatgcagccg
//
LOCUS       DAZXUL010000384          803 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZXUL010000384 DAZXUL010000000
VERSION     DAZXUL010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.394"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            46..803
                     /locus_tag="ACH3L1_004732"
                     /pseudo
     CDS             46..803
                     /locus_tag="ACH3L1_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          234 a          169 c          198 g          202 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa
      541 gccttagctc atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc
      601 ttgatgagct tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg
      661 ctctcagcaa gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg
      721 agaatagagg ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac
      781 agattaacca taaagctgtt cac
//
LOCUS       DAZXUL010000385          798 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZXUL010000385 DAZXUL010000000
VERSION     DAZXUL010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.395"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(135..265)
                     /locus_tag="ACH3L1_004733"
                     /pseudo
     CDS             complement(135..265)
                     /locus_tag="ACH3L1_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            263..346
                     /gene="tnpB"
                     /locus_tag="ACH3L1_004734"
                     /pseudo
     CDS             263..346
                     /gene="tnpB"
                     /locus_tag="ACH3L1_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          226 a          148 c          192 g          232 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatatta cttccttatg ttatattttg gtaaaaaatt
       61 tattttttta cattatagtc ggctaaatga agtttactgt aaaaaaacga tactttttcg
      121 ggtggcgctt ccaatcagta gaatatgtgt ttttcaatga aggagccgat gacttttcgt
      181 ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc
      241 ggagggtcag gttgttacgt tcaagatata gcatgtgctg gatgaagatc ccttttctgg
      301 tcacctgttt atctttcgtg gccgacgggg tgacgctgtt aaaatgtaat gaacgcatcc
      361 cacacaatta aacacggagt agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
      421 ctcgcagctt tccggcatca tactgcccaa tacgggatgc ataaagcgtc ctcagactgg
      481 agactccaca aatgaaatat acaccggttg gcgttgatat ggcaaaacat gtcattcaga
      541 ttcacttcat caatgagcac atgtcagcgc cagtgatata agacggtaat ggggatgcgg
      601 acgatttcct ggactctcag aagatgaaac atgtttactg aagaacaaaa aattcgcgct
      661 attgagcttt atttcaaata tggtaagaag cttgaggtgt actggcaata gcggacacca
      721 ccatttgttc tttttctaag cagtcatctg ataatatttt atcctgaagg ctgccgggga
      781 gatattcccc agacgaga
//
LOCUS       DAZXUL010000386          784 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZXUL010000386 DAZXUL010000000
VERSION     DAZXUL010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.396"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(3..76)
                     /locus_tag="ACH3L1_004735"
     tRNA            complement(3..76)
                     /locus_tag="ACH3L1_004735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Arg,seq:tct)
     gene            complement(78..154)
                     /locus_tag="ACH3L1_004736"
     tRNA            complement(78..154)
                     /locus_tag="ACH3L1_004736"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(118..120),aa:Ile,seq:cat)
     gene            complement(344..>784)
                     /locus_tag="ACH3L1_004737"
     CDS             complement(344..>784)
                     /locus_tag="ACH3L1_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFT4079111.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          197 a          218 c          152 g          217 t
ORIGIN      
        1 ggcccaccac cgaggactcg aacctcgcac cgtcaactta gaaggttgat gctctatccg
       61 gatgagctag tggtggttgg tggcccttgc tggacttgaa ccagcgacct ggcgattatg
      121 agtcgctcgc tctgaccaac tgagctaaag ggccggaggc agaataataa ccatatgtca
      181 tcacatctgc aaactcatct gatcaccagc acgtttaacg tcctgtgccg tttttcaggt
      241 ataaaaaaaa ccgcataaag cgggctcttt caaatgtcca tgtctgctat tcgcctcgcg
      301 gtacagcttt gcgaagcgta caagaattga agcagttttt acgtcaaaaa gcaataactt
      361 ttttctctat accaaaagcc ataaccattg gtttgtacaa aataaattct gccaccttta
      421 gccaatgctc aatgcgtctt tcacaggttc ttaaactcca tttcgggtgt gcatcattca
      481 gcagttcagc cattttgcat ttggtcatcc cccgcccctc ataccgttgc cgaaggacgc
      541 taatcaatcc tggatgctct gccagcacct cacttatgac ccgatcaata cataacgcct
      601 ctgcatcagt acaatgcgcc agccagctct tttgcttacc gttaatcata tcccgcaaaa
      661 aagcctcaag ttcagacttg ttcagacctg cttttttcat cctccggagc gcctcgttaa
      721 ttgccgtttt tgtcagcttt ttagaggtca acatcaggtt aaacatattc cccgtcttac
      781 cgcc
//
LOCUS       DAZXUL010000387          762 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZXUL010000387 DAZXUL010000000
VERSION     DAZXUL010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.397"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(34..>762)
                     /gene="blaOXA"
                     /locus_tag="ACH3L1_004738"
     CDS             complement(34..>762)
                     /gene="blaOXA"
                     /locus_tag="ACH3L1_004738"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HFT4079112.1"
                     /translation="PLFEGTEGCFLLYDASTNAEIAQFNKAKCATQMAPDSTFKIALS
                     LMAFDAEIIDQKTIFKWDKTPKGMEIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIK
                     NYLKDFDYGNQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNLPVKNSAI
                     ENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVSALTGN
                     LGSNLTSSIKAKKNAITILNTLNL"
BASE COUNT          191 a          130 c          137 g          304 t
ORIGIN      
        1 ttgaagggtt gggcgatttt gccattagat tttttataaa tttagtgtgt ttagaatggt
       61 gatcgcattt ttcttggctt ttatgcttga tgttaaattc gaccccaagt ttcctgtaag
      121 tgcggacaca aaaacatatt tatgtcctga tttgcttata ataaaccctt caaaccatcc
      181 gttttgtaag gttctatttg ctgtgaatcc tgcaccagtt ttcccataca gttttgtact
      241 attatccaga tcttgtagat acatgttctc tatggtgttt tctatggctg agtttttaac
      301 tgggagattg tgattaataa ttttacgcag gaattgaatt tgttcttctg gtgaaatttt
      361 taagctactt tcgagccatg cttctgttaa tccgttgttt ctttctttat ctccagagaa
      421 gtcttgattt ccataatcaa aatctttgag ataattcttg attttattta atccaatttt
      481 ttgggttatt tcttgcgaaa cccaaacaac agaaaattgc atccacgtct ttggtgtatg
      541 attgctgttc cagatctcca ttcctttggg ggttttatcc catttgaata tggttttctg
      601 atctattatt tccgcatcaa atgccataag tgataatgcg atcttgaaag ttgaatctgg
      661 tgccatttgc gttgcacact ttgctttatt gaattgagca atttcagcgt ttgtggatgc
      721 atcgtaaagt aaaaaacaac cttcagttcc ttcaaataat gg
//
LOCUS       DAZXUL010000388          760 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZXUL010000388 DAZXUL010000000
VERSION     DAZXUL010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.398"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>760
                     /locus_tag="ACH3L1_004739"
     CDS             <1..>760
                     /locus_tag="ACH3L1_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFT4079113.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          185 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagcag
//
LOCUS       DAZXUL010000389          717 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZXUL010000389 DAZXUL010000000
VERSION     DAZXUL010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.399"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(54..717)
                     /locus_tag="ACH3L1_004740"
                     /pseudo
     CDS             complement(54..717)
                     /locus_tag="ACH3L1_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          207 a          147 c          138 g          225 t
ORIGIN      
        1 cagtgggtat atctgttcag aatatttcac gtagcacacg gatatataag aaatcattct
       61 tcctctggat agcttgaact taagagcata tcctgcgtga attggtcatt agctgattga
      121 ttgaagctaa agaaagatgg tgcatccggt ctaacagttg cttcaccagt gatattaact
      181 gataaaggct tcttagtttc tgctgttaaa gcgtctacct ttcttcctaa ctctgataca
      241 gccatagcta attgagcatt gctattaacc atgtcacctg tgatagctga tggagttgat
      301 tgtgtgttat ttacgaagtt attcatcggt tcttggaagt tagcaatttt attagttgca
      361 ccattaaacc agtcagcgat agaactattg gcttagatga atcacctttg tattcagggt
      421 tgaagtttgt ttctgtgcca atacctgatt gatgtaagtt cggattctta gctgcttcag
      481 ggtttaaatt atcaagagca aaatttacca actcagcata aaggacaact ctaccgactt
      541 tatttagcgc accacctgaa gaaccagcct tgccagcttt agctttatta acagcgtcac
      601 cgaatgaagt aacagcgttt gcaaggttta acaagcctcc tgctacattt gcagcctttt
      661 gagcagtaac agccacagca agcatcttca gaccatcaat taacccatca gataaac
//
LOCUS       DAZXUL010000390          709 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZXUL010000390 DAZXUL010000000
VERSION     DAZXUL010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.400"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            45..506
                     /locus_tag="ACH3L1_004741"
     CDS             45..506
                     /locus_tag="ACH3L1_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFT4079114.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(547..>709)
                     /locus_tag="ACH3L1_004742"
     CDS             complement(547..>709)
                     /locus_tag="ACH3L1_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFT4079115.1"
                     /translation="ILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGL
                     AARQHVKKS"
BASE COUNT          208 a          158 c          161 g          182 t
ORIGIN      
        1 agcattaccc accagtttct tgattaaata aaatggagtt ttacatgaag gctttcaata
       61 agctgttttc cctcgttgtt gcatctgttc tggttttctc tcttgctggc tgcggtgaca
      121 aagaagaatc gaagaaattc agcgccaatc tgaacggcac tgaaattgcc attacctatg
      181 tctacaaagg tgacaaggtg cttaagcaat cttctgaaac caaaattcaa tttgcctcca
      241 ttggtgcaac caccaaagaa gatgctgcca agacacttga gccgttaagc gccaaataca
      301 aaaacatcgc gggtgttgaa gaaaaatcaa cctataccga tacctacgcg caggaaaacg
      361 tgactatcga tatggaaaaa gtggatttta aagccctgca gggtatttca ggaatcaacg
      421 tttctgctga agatgccaaa aaaggtatca ctatggcgca aatggaactg gtgatgaaag
      481 ccgctggttt taaagaagtg aaataatctg tcggcggcca tgtttcgcat ggccgccata
      541 cccgtcttag cttttcttca catgctggcg cgctgccagt ccacgcagaa aataacgtaa
      601 aaattgatcg ccgcattcgc ggaagttttt atgatccggt gcgcgcatca tcgctgtaat
      661 ttccggcatc gaaacgcgga actgctgttc ggtgaggata gccagaatg
//
LOCUS       DAZXUL010000391          705 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZXUL010000391 DAZXUL010000000
VERSION     DAZXUL010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.401"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..443)
                     /locus_tag="ACH3L1_004743"
     CDS             complement(<1..443)
                     /locus_tag="ACH3L1_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFT4079116.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          215 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttcaa
//
LOCUS       DAZXUL010000392          702 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZXUL010000392 DAZXUL010000000
VERSION     DAZXUL010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.402"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>702
                     /locus_tag="ACH3L1_004744"
     CDS             <1..>702
                     /locus_tag="ACH3L1_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFT4079117.1"
                     /translation="ICERFFFWQLRSFKLDVRGYARHFKVLNSCLFALPPIAEQERIV
                     EKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQNTAELAENWARISEHFDTL
                     FTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKP
                     LPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYLNLYQVAPDIIEKGTGRVIS
                     KRTVKESGVKGPNSRF"
BASE COUNT          234 a          140 c          159 g          169 t
ORIGIN      
        1 atttgtgagc gtttcttttt ttggcagtta cgatcattta aattagatgt aagaggatac
       61 gcgagacatt ttaaagtatt aaatagctgc ttatttgcat taccccccat tgcagaacaa
      121 gagcgaatag ttgaaaaagt tagctctttg atgtccctct gcgaccaact agaacagcaa
      181 tccctgacca gtctggacgc acatcagcaa ctggttgaaa ccctgttggg aacgcttaca
      241 gacagccaaa acaccgcgga actggctgaa aactgggcgc gtattagcga gcatttcgac
      301 accctattta ccaccgaagc cagcgtggat gcgttaaaac agaccattct gcaactggcc
      361 gtaatgggca aacttgtgcc gcaggatccg aatgacgaac ccgcctctga actgctcaaa
      421 cgtattgcgc aggaaaaagc tcaattggtg aaagaaggga aaataaaaaa acaaaaacca
      481 ctgccgccaa ttagcgatga ggaaaaaccg tttgaacttc cggaagggtg ggaatggtgt
      541 cgtattggta atatagttaa tattaaatct gaattagtat caccaaaaga ttatttaaat
      601 ttatatcaag tagcacctga tattattgaa aagggcactg ggagagtgat atcaaagaga
      661 acagtgaaag aatcaggagt caaagggcca aatagtcgct tt
//
LOCUS       DAZXUL010000393          698 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZXUL010000393 DAZXUL010000000
VERSION     DAZXUL010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.403"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          278 a          165 c           89 g          166 t
ORIGIN      
        1 aacagtcaat tattcatctc cactggcgag cacggtcagc acacaaacaa agttagtcct
       61 cctgcttaat cattgcgtat cagaaactaa aaaagaaaaa attctgcaga caacttaaca
      121 atcttcatca catttcctga agacagacat atccacaaac ggtagtcagc gttgaattaa
      181 caagtcttac agagctatac gtagcttata aagccactca atgttcatca tcacccgact
      241 acttatctgg aataccacca gtataaactc atcaataaaa acacctgttg ttaaaataag
      301 aatactgtca gcagtaaatt attaaaacaa catctgtcca cactatctgt tcttttaacg
      361 ccaacggcag cgaaaatagt gcatcacagc cttctttttt ctttcgccac cactcagaca
      421 aacacaacac tcacacccac accacaaaac agaatcaaaa cataaaatta acaataacag
      481 tcaaaaggaa aacacagaat attgaggtgg actgtcacca ggacagcgca tacgacgcat
      541 gtgatcagca catcccgttc ttcgctggca tccccaaaat gcagaacaaa caatcataac
      601 attatgaata taaaagaaaa tatgattttt caccatgaaa aaaacatcaa tgtatctctg
      661 tataaaaata atgataacat gacataaaca tttaaaaa
//
LOCUS       DAZXUL010000394          693 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZXUL010000394 DAZXUL010000000
VERSION     DAZXUL010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.404"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..525
                     /locus_tag="ACH3L1_004745"
                     /pseudo
     CDS             1..525
                     /locus_tag="ACH3L1_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>693
                     /locus_tag="ACH3L1_004746"
     CDS             589..>693
                     /locus_tag="ACH3L1_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFT4079118.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDIT"
BASE COUNT          193 a          143 c          166 g          191 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tctcgatatc acc
//
LOCUS       DAZXUL010000395          690 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZXUL010000395 DAZXUL010000000
VERSION     DAZXUL010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.405"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            2..>690
                     /locus_tag="ACH3L1_004747"
     rRNA            2..>690
                     /locus_tag="ACH3L1_004747"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          200 a          147 c          210 g          133 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga
      301 agcgtctgga aaggcgtgcg atacagggtg acagccccgt acacaaaaat gcacatactg
      361 tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct
      421 ccaaggctaa atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa
      481 agaaccccgg cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag
      541 cacgcttagg cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta
      601 gcaaggttaa ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg
      661 cagggtatag acccgaaacc cggtgatcta
//
LOCUS       DAZXUL010000396          685 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZXUL010000396 DAZXUL010000000
VERSION     DAZXUL010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.406"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            52..685
                     /locus_tag="ACH3L1_004748"
                     /pseudo
     CDS             52..685
                     /locus_tag="ACH3L1_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DAZXUL010000397          682 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZXUL010000397 DAZXUL010000000
VERSION     DAZXUL010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.407"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..204
                     /locus_tag="ACH3L1_004749"
                     /pseudo
     CDS             1..204
                     /locus_tag="ACH3L1_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(270..635)
                     /gene="istB"
                     /locus_tag="ACH3L1_004750"
                     /pseudo
     CDS             complement(270..635)
                     /gene="istB"
                     /locus_tag="ACH3L1_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
BASE COUNT          172 a          156 c          170 g          184 t
ORIGIN      
        1 atgacaggcg aaaatcgtgg ccggggaagg ccgcgaaaga cagaaagtac ctatgcagat
       61 actcgtaatg atctgatccg cagtggcctt gaactgctga cgcagaatgg ttttcttgcg
      121 acgggagtcg atgccattgt taaaaatgcc aatgtcccta aaggctcatt ttattactac
      181 cttaagagta aagaggatta tgctgtcaac gacggatgaa aagtgatcca cttatatctc
      241 caccaacggc ccaatattga tccaccgttt tactcaggat tagcttctgc tataaccccg
      301 gcctttcgtt tctgtctgag tcgatagctt tctcctttga tttgaacgat atgtgagtgg
      361 tgtaagatac ggtccagcat cgctgaggtc agtgctgcat caccggcgaa cgtttgatcc
      421 cactgcccga acggcagatt ggatgtcagg atcattgcgc tcttttcgta acgtttagcg
      481 atgacctgga agaacagctt tgcttcttcc tgactgaacg gcagatagcc tatttcatca
      541 atgatgagca ggcggggggc cattactcca cgctgaagcg tcgttttata acggccctga
      601 cgttgtgccg tagataactg aagtaacaga tctgcgtaag cgtcaacgga gcaccgtatt
      661 gacgcttatt tattggtgag ta
//
LOCUS       DAZXUL010000398          679 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZXUL010000398 DAZXUL010000000
VERSION     DAZXUL010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.408"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..251)
                     /locus_tag="ACH3L1_004751"
     CDS             complement(<1..251)
                     /locus_tag="ACH3L1_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFT4079119.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>679)
                     /locus_tag="ACH3L1_004752"
     CDS             complement(272..>679)
                     /locus_tag="ACH3L1_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4079120.1"
                     /translation="HRALSEHKTLSVSWSHYRSTVHKCTFVFSPQVIKQRQFSGPHYK
                     VDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNV
                     YGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
BASE COUNT          155 a          156 c          152 g          216 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gtacttcggt aatgactcca acttactgat agtgttttat
      661 gttcagataa tgcccgatg
//
LOCUS       DAZXUL010000399          668 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZXUL010000399 DAZXUL010000000
VERSION     DAZXUL010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.409"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>668
                     /locus_tag="ACH3L1_004753"
     CDS             <1..>668
                     /locus_tag="ACH3L1_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFT4079121.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAZXUL010000400          660 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZXUL010000400 DAZXUL010000000
VERSION     DAZXUL010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.410"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..131
                     /locus_tag="ACH3L1_004754"
     CDS             <1..131
                     /locus_tag="ACH3L1_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFT4079122.1"
                     /translation="ERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            246..>660
                     /locus_tag="ACH3L1_004755"
     CDS             246..>660
                     /locus_tag="ACH3L1_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFT4079123.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPS"
BASE COUNT          168 a          177 c          171 g          144 t
ORIGIN      
        1 ttgaacggtg ctttgggcgg ctgaaagaat atcgccgcat cgccacgcgt tacgacaaaa
       61 cggcgagaaa ttacctggcg atggtgaaac tgggctgcat ccgactcttt tatcaacgct
      121 tacgtaatta agggacacag cctaaatagc tgcgcggaat agtagatccc tttgagggaa
      181 cttagcccgg attgtgcgat ctgatcaatc gccaaatcaa aacaaatcac caaccggact
      241 gagcaatgcc gatcatagca ccaatttccc gtgacgaacg acgcctgatg cagaaagcca
      301 tccataaaac acacgataaa aattatgccc gcagactgac tgccatgctg atgctgcacc
      361 ggggcgaccg tgtcagcgac gttgccagaa cgctctgctg cgcccgttcc tctgttggac
      421 gctggattaa ctggttcacg cagtcgggtg ttgagggact gaaatcatta cctgccgggc
      481 gtgcccgtcg ctggccgttt gagcatatct gcacactgtt acgtgagctg gtaaaacatt
      541 ctcccggcga ctttggctac cagcgttcac gctggagtac agaactgctg gcaataaaaa
      601 tcaatgagat aaccggctgc cagttaaatg ccggaaccgt tcgccgctgg ttgccgtctg
//
LOCUS       DAZXUL010000401          659 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZXUL010000401 DAZXUL010000000
VERSION     DAZXUL010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.411"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            303..378
                     /locus_tag="ACH3L1_004756"
     tRNA            303..378
                     /locus_tag="ACH3L1_004756"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:336..338,aa:Ile,seq:cat)
BASE COUNT          195 a          116 c          154 g          194 t
ORIGIN      
        1 agtgaaaagt aacagatatc ataatgatgc gaaaactctt cagctaatgt aataaagtta
       61 tattattaga tacaaaatca ttacaaagta tcattgaatc gatgataact atagatagtg
      121 ctaattatta acatttgctt gttgactgcg agtatcgact ttgcgaatgg aggcggtgtc
      181 gggttttatg tcgcgtgtaa tcacgaccca aggtgaatgg gaacgcataa aaatctgtta
      241 tttacatcat aagatcatca tgtttattgc gtggtttata agtattgtta ttgcatactt
      301 aaggcccttt agctcagtgg ttagagcagg cgactcataa tcgcttggtc gctggttcaa
      361 gtccagcaag ggccaccaaa tcatcattca tgtatcagga agagttaagg gtacaagggg
      421 tactcgatgg cggtctatat ttcatgacta acgccatttc aaggaagaag tgcggtgggt
      481 ggtttcgaat gagggctggt gttttggtac gtggcacgcc actgcaatgt atttattcat
      541 cagtactcat tcaaaagtac ctggaaaaaa tgcccgctat gccaagcggg caaattgatc
      601 ctacccacgt aatatggaca caggcctaag cgaggttctg gttttcaaat tgttccgga
//
LOCUS       DAZXUL010000402          656 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZXUL010000402 DAZXUL010000000
VERSION     DAZXUL010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.412"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(151..>656)
                     /locus_tag="ACH3L1_004757"
     CDS             complement(151..>656)
                     /locus_tag="ACH3L1_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFT4079124.1"
                     /translation="PVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDL
                     KELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAZXUL010000403          640 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZXUL010000403 DAZXUL010000000
VERSION     DAZXUL010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.414"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..142)
                     /gene="csgC"
                     /locus_tag="ACH3L1_004758"
     CDS             complement(<1..142)
                     /gene="csgC"
                     /locus_tag="ACH3L1_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HFT4079125.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLR"
     gene            complement(201..566)
                     /gene="csgA"
                     /locus_tag="ACH3L1_004759"
     CDS             complement(201..566)
                     /gene="csgA"
                     /locus_tag="ACH3L1_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFT4079126.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT          166 a          146 c          155 g          173 t
ORIGIN      
        1 cgcgcaggga caatatttgt actctgcaca gacaagattg agtaagagta acttcaggaa
       61 taatggtgta catatcccct tgctgggtcg tattaaaggt tatctggctg gaaagtgccg
      121 caaggagtaa taacgcattc atattcttct cccgaaaaaa aacagggctt gcgccctgtt
      181 tctgtaatac aaatgatgta ttaatactga tgagcggtcg cgttgttacc aaagccaacc
      241 tgagtcacgt tgacggagga gttcgatgca gtctggtcaa ctgcagcacc gttgccacca
      301 ccgaactgtt taacagtcat ttcagaattt ttgccgttcc actgatcaag agtggcgctg
      361 ttaccgaagc cacgttgggt cagatcgatt gagctgtcat ctgagccctg accaacatct
      421 gcaccattac caccgccatg ctgggtaata gtcaagtcag agttacgggc atcagtttgc
      481 agagcaagtg cagagttacc gccaccgtac tggtaaatgt tcagctctga atttgggccg
      541 ctattattac tgaggtgtac tggcaatagc ggacactacc atttgttctt ttttaagcag
      601 ccatctgatg atatttttcc ctgaaggctg ccggggagat
//
LOCUS       DAZXUL010000404          618 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZXUL010000404 DAZXUL010000000
VERSION     DAZXUL010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.415"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>618)
                     /gene="rfbG"
                     /locus_tag="ACH3L1_004760"
     CDS             complement(<1..>618)
                     /gene="rfbG"
                     /locus_tag="ACH3L1_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HFT4079127.1"
                     /translation="YICFSDQDDIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRK
                     NLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYS
                     FARTRNYSWYIDRKPTMLYRQHENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANA
                     LADDSFVNNQLGKGYIGNLILALSFWKLRRKKADKIYILLMLILNI"
BASE COUNT          197 a          123 c           73 g          225 t
ORIGIN      
        1 tatgtttaaa atcaacatta ataaaatata aattttatca gcctttttcc gccttaactt
       61 ccaaaaactt aaagccaaga tcaaattgcc tatataacct ttacccaatt gattattcac
      121 aaagctatca tcagctaagg cattagctat tttagttact tcttttcgat accacttgtt
      181 tctcaccaaa ccaagccgtt tgtattttgc tttaaaacta atgtttgctc ccacttggtt
      241 gttttcatgc tgtctgtaca gcattgttgg ttttctatca atataccacg aataatttct
      301 cgtccgcgca aatgaatata aaaaccaatc atgcagacag atatcttgtg cagcattttt
      361 attatttatg atgaacttct taaattcaat aagcgtttct ttttttataa catatgtaca
      421 ccccggacct gctgcttcaa aaaaataatc gaattgagtt tgcgactgag ccttatcaac
      481 taaatttttt cgccctgatg gataatatgc aattacattg ctggaataac aattcgcatt
      541 attaaatacc aagcagtcaa ttgcattttt tattttatct ttataccaaa tatcgtcctg
      601 atcactaaaa cagatata
//
LOCUS       DAZXUL010000405          596 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZXUL010000405 DAZXUL010000000
VERSION     DAZXUL010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.416"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            12..>596
                     /locus_tag="ACH3L1_004761"
     CDS             12..>596
                     /locus_tag="ACH3L1_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFT4079128.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          130 a          159 c          172 g          135 t
ORIGIN      
        1 ccgcccttct gatgctcccc cgttttgccg atatttttca gcagggaaac cgctggctta
       61 actggctgga gaaacaaccg gaaggggcag tccgtccggt ggtaattgaa tcagtgacaa
      121 aaatcatggc ctgcgggacc acactgatgg gctacacgca gtggtgttgt tcatcaccgg
      181 actgttgcca tacaaaaaag gtctgcttcc ggtgtaaaag ccgctcctgc ccgcactgcg
      241 gggtgaaggc tggcgcacag tggatacagt atctgctgag cctggtcccc tactgcccgt
      301 ggcagcatat tgtgttcaca cttccctgcc agtactggtc cctggtgttc cacaaccggt
      361 ggttactggc agagatgagc cgcattgcag cggatgtgat actggaaatc tgccatcagg
      421 cagatgtgga gccggggata ttcacggtga tccacacatg ggggcgtgac cagcagtggc
      481 atccgcatat ccatttatcg acaactgccg gtggtgtgac gtcgggccac acctggaaaa
      541 atcttcattt ttacgcccgt aaggtgatga gcatgtggcg ctaccggata acacgg
//
LOCUS       DAZXUL010000406          577 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZXUL010000406 DAZXUL010000000
VERSION     DAZXUL010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.417"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..98
                     /locus_tag="ACH3L1_004762"
     CDS             <1..98
                     /locus_tag="ACH3L1_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFT4079129.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="ACH3L1_004763"
     CDS             156..512
                     /locus_tag="ACH3L1_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079130.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          183 a          110 c          149 g          135 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatca
//
LOCUS       DAZXUL010000407          557 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZXUL010000407 DAZXUL010000000
VERSION     DAZXUL010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.418"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>557
                     /locus_tag="ACH3L1_004764"
     CDS             <1..>557
                     /locus_tag="ACH3L1_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFT4079131.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVS"
BASE COUNT          164 a          137 c          148 g          108 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc gtcagcc
//
LOCUS       DAZXUL010000408          555 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZXUL010000408 DAZXUL010000000
VERSION     DAZXUL010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.419"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>555
                     /gene="ycjM"
                     /locus_tag="ACH3L1_004765"
     CDS             <1..>555
                     /gene="ycjM"
                     /locus_tag="ACH3L1_004765"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFT4079132.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLR"
BASE COUNT          170 a          105 c          124 g          156 t
ORIGIN      
        1 tagtaaactg gaaaaataat cccgacggca cgcgcagtcc ctatgagatg aatgtcacct
       61 atatgaatgc gttaagccgc cgcgagagta gcgatgaaga acgttgcacc aggtttatcc
      121 ttgcccatgc gattttgtta agtttccccg gtgtacccgc gatatatatt caaagtattc
      181 ttggctcgcg taatgattac gcaggtgtcg aaaaactcgg atataaccgt gcgattaacc
      241 gtaaaaaata tcacagtgaa gagataacac gagaactgaa cgatgaagct acattaaggc
      301 atgcggtata tcatgagttg tcgcgtttaa ttacacttcg tcgcagccat aacgagtttc
      361 atccggataa taattttacc atcgatacgg ttaattcatc cgtaatgcgt attcaaagaa
      421 gtaacgctga tggtaattgt ctgactggat tgtttaatgt cagtaaaaat attcagcatg
      481 taaatattac tgatctgcat ggtcgggatc tgattagtga agttgatata ttgggtaatg
      541 aaataacgct gcgcc
//
LOCUS       DAZXUL010000409          552 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZXUL010000409 DAZXUL010000000
VERSION     DAZXUL010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.420"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>552)
                     /locus_tag="ACH3L1_004766"
     CDS             complement(<1..>552)
                     /locus_tag="ACH3L1_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HFT4079133.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIK"
BASE COUNT          134 a          117 c          135 g          166 t
ORIGIN      
        1 atttaatatc aatctgttga aggtgtatta ttacatcagt agcatattcc agactgcctt
       61 cctggacgat ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa
      121 gtgccgtgag tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct
      181 gactgatcgc ttgggcgacg gtgttttgcc attctaaaga acaggtgctg aagtcaaatg
      241 tggctgtttt attatcccgt tgcgacacgc tacaggctaa gcgacgtaat tgggtcaatt
      301 tgtctgcttg ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa
      361 cttcgggcgg aaaaacgaca gcaatatctt tcgccagcca aggtaaaaca gcatctgtat
      421 ttttgtcagc cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata
      481 agtccgctac cttgagagag tccgttaccg tttgggaagg ttgcaaagaa ttatctttcg
      541 cttgctccac at
//
LOCUS       DAZXUL010000410          544 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZXUL010000410 DAZXUL010000000
VERSION     DAZXUL010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.421"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            155..340
                     /locus_tag="ACH3L1_004767"
                     /pseudo
     CDS             155..340
                     /locus_tag="ACH3L1_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(440..>544)
                     /locus_tag="ACH3L1_004768"
     CDS             complement(440..>544)
                     /locus_tag="ACH3L1_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFT4079134.1"
                     /translation="AFQLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
BASE COUNT          155 a          131 c          136 g          122 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaaggg gatatatcgc
      421 agctctgacg actcactcct tatttacccg ggaaatactt ctccagcaat gcatacagga
      481 tggcagcggt ttcagcatcc attatgccgt cataattttt ctgccggaaa tgaagctgaa
      541 aagc
//
LOCUS       DAZXUL010000411          537 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZXUL010000411 DAZXUL010000000
VERSION     DAZXUL010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.422"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..264)
                     /locus_tag="ACH3L1_004769"
     CDS             complement(<1..264)
                     /locus_tag="ACH3L1_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFT4079135.1"
                     /translation="MRDVQWFCPLTPVHTGTEIKPVEMPVTTITPVSDAGGLQDFIYW
                     RPDADRAGMEPVYVILNDPLDSGRFTRKQLNKNILNMQVILVFS"
BASE COUNT          143 a          131 c          119 g          144 t
ORIGIN      
        1 cgagaatacc aaaattactt gcatgtttaa gatattttta tttagttgtt tacgcgtaaa
       61 tcgcccggaa tccagaggat cattaagtat cacataaaca ggttccatac cagccctgtc
      121 cgcatcagga cgccagtaaa taaaatcttg tagtccaccg gcatcagaga caggagtaat
      181 cgtcgttacc ggcatttcga ctggttttat ttccgtacct gtatgtactg gtgtgagcgg
      241 acaaaaccac tgtacgtctc tcatacacgc tcgtaagcgg ccctcacggc ccgctaacgc
      301 ggagatacgc cccgacttcg ggtaaaaccc tcgtcgggac cactccgacc gcgcacaaaa
      361 gcattttcat cgccgatagc gggtatggct tggcagggcc gggatgggta taggtaattt
      421 ggctatcgta aaaaaggtgg ttaaaaatta caacctgcaa tgtaaattgg ctagttaatt
      481 tttggctatt taaattgact tcttaacggc gtttactgtt ccattgctcc ccaaccc
//
LOCUS       DAZXUL010000412          537 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZXUL010000412 DAZXUL010000000
VERSION     DAZXUL010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.423"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>537)
                     /locus_tag="ACH3L1_004770"
     CDS             complement(<1..>537)
                     /locus_tag="ACH3L1_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFT4079136.1"
                     /translation="NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQF
                     STNEIAAVVAERDIEWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEM
                     LRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFI
                     HYVGPTKPWHEWADYPVSR"
BASE COUNT          171 a          101 c           82 g          183 t
ORIGIN      
        1 cgtgaaaccg gataatcagc ccactcatgc cagggttttg taggtcccac atagtgtata
       61 aacacggtgt catcattgac tggattatca actttgtctt ttaattcata gttaatacta
      121 tatcgggtat tatatttctc cgaaataaac ttcactttac cattcaataa tacattcagt
      181 acatcttgat caaggtgggt gattttactt acccaatctg ggtcacgcaa catttcaata
      241 gcttttgacg aaatgttatt tagattccac tcatcaatat ttatcagtaa aaaaccagca
      301 ttgaaatatc cagaagctaa ctgtggtgta gttaataccg aggctcgatt ttgccaccat
      361 tcaatatctc tttcagctac aactgcggca atttcattag tagaaaattg ataatctaag
      421 agttctttaa tactaccctt gcaagcaata tctgcatcaa gatatagtat tttttcatgt
      481 ttatgataga aataatctgc aattataaat cgaaaatatg ttgcgtaagt ccagttt
//
LOCUS       DAZXUL010000413          511 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZXUL010000413 DAZXUL010000000
VERSION     DAZXUL010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.424"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..511
                     /locus_tag="ACH3L1_004771"
                     /pseudo
     CDS             1..511
                     /locus_tag="ACH3L1_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAZXUL010000414          493 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZXUL010000414 DAZXUL010000000
VERSION     DAZXUL010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.425"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>493
                     /locus_tag="ACH3L1_004772"
     CDS             <1..>493
                     /locus_tag="ACH3L1_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFT4079137.1"
                     /translation="RTRETYGTRRLQTELADNGIIVGRDRLARLRKELRLHCKQKRKF
                     RATTNSDHNLPVTPNLLNQNFTPTAPNQVWVADITYVATREGWLYLAGVKDVYTCEIV
                     GYAMGERMMKELTGKALFMALRSQRLPVGLIHHTDRGSQYCAYDYRVIQEQSGLKTSM
                     SRKG"
BASE COUNT          129 a          127 c          137 g          100 t
ORIGIN      
        1 tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca gataacggaa
       61 ttatcgtcgg tcgtgacaga ctggcacgtc ttcgtaagga gctgaggctg cactgtaagc
      121 agaaacgcaa gttcagggcg accacaaact ctgaccacaa tctgccggtt acgccgaatc
      181 tgctgaatca gaactttacg cccacagcac ctaatcaggt ctgggtcgca gatataacgt
      241 atgttgccac ccgggaagga tggctgtatc tcgccggcgt caaagatgtt tatacgtgcg
      301 aaattgtcgg ttacgccatg ggagagcgca tgatgaaaga gttgacgggt aaagccctgt
      361 ttatggcgct caggagccag cgcctacctg tcgggctaat ccaccacacc gatcgaggtt
      421 cacagtactg cgcatacgat tacagggtca tacaggagca gtctggtctg aaaacatcaa
      481 tgtcgcgtaa agg
//
LOCUS       DAZXUL010000415          488 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZXUL010000415 DAZXUL010000000
VERSION     DAZXUL010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.426"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..488
                     /locus_tag="ACH3L1_004773"
                     /pseudo
     CDS             1..488
                     /locus_tag="ACH3L1_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          106 a          120 c          152 g          110 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgctggactg
      481 ctgtgatc
//
LOCUS       DAZXUL010000416          483 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZXUL010000416 DAZXUL010000000
VERSION     DAZXUL010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.427"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..483
                     /locus_tag="ACH3L1_004774"
                     /pseudo
     CDS             1..483
                     /locus_tag="ACH3L1_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          177 a           81 c           95 g          130 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aaagtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaa
//
LOCUS       DAZXUL010000417          472 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZXUL010000417 DAZXUL010000000
VERSION     DAZXUL010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.428"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            3..>472
                     /locus_tag="ACH3L1_004775"
     CDS             3..>472
                     /locus_tag="ACH3L1_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFT4079138.1"
                     /translation="MDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAZXUL010000418          471 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZXUL010000418 DAZXUL010000000
VERSION     DAZXUL010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.429"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..361
                     /locus_tag="ACH3L1_004776"
     CDS             <1..361
                     /locus_tag="ACH3L1_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFT4079139.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT          128 a           99 c          161 g           83 t
ORIGIN      
        1 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
       61 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
      121 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataaacc
      181 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
      241 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccatcg cgggactgaa
      301 tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
      361 acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg ttgcggtata
      421 agaatcgacc atgcagagag agaaaacacc ggagtggagg gaaaagcaga a
//
LOCUS       DAZXUL010000419          468 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZXUL010000419 DAZXUL010000000
VERSION     DAZXUL010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.430"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>468
                     /locus_tag="ACH3L1_004777"
     CDS             <1..>468
                     /locus_tag="ACH3L1_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFT4079140.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASAN"
BASE COUNT          148 a           96 c          103 g          121 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agctaatg
//
LOCUS       DAZXUL010000420          460 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZXUL010000420 DAZXUL010000000
VERSION     DAZXUL010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.431"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>460)
                     /locus_tag="ACH3L1_004778"
     CDS             complement(<1..>460)
                     /locus_tag="ACH3L1_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFT4079141.1"
                     /translation="ILSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLL
                     MFNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVR
                     EKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          147 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaat
//
LOCUS       DAZXUL010000421          448 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZXUL010000421 DAZXUL010000000
VERSION     DAZXUL010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.432"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..382)
                     /locus_tag="ACH3L1_004779"
     CDS             complement(<1..382)
                     /locus_tag="ACH3L1_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079142.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLL"
BASE COUNT          135 a          106 c           96 g          111 t
ORIGIN      
        1 ttaacagact tgattcatcc tcacaaaata taagtttttc ctggaatgcc catgtctcat
       61 tgaggttcca gatatcaaat tcgaagtcgt gataacgaaa acggatccca ccaaacttat
      121 taaaacgcag ttgtttgacg ggcagttcag ccaatgtctg gaagagttcc tcacgggagc
      181 gaccgatgac gatatcaagg tctgaagaga atccatcgac gccatacaag cctaagtcac
      241 gcaccatccc gccaaaaatc ctggccggac aggaacaggc cagagtatca agtaatactc
      301 tggctttctt atgctcatca ctaagcgtaa aaaaatcggc gatacgctgt tcaagcagtt
      361 gggtgtttga gaccacattc atacagtgat tttagctcat cgtgaaggcg cattaaataa
      421 ctatccgtca tgcctgagat tgcgtcgg
//
LOCUS       DAZXUL010000422          444 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZXUL010000422 DAZXUL010000000
VERSION     DAZXUL010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.433"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            16..444
                     /locus_tag="ACH3L1_004780"
                     /pseudo
     CDS             16..444
                     /locus_tag="ACH3L1_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
BASE COUNT           96 a          125 c          122 g          101 t
ORIGIN      
        1 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
       61 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
      121 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
      181 atcattgata tggccatgaa tggcgttgga tgccgggcaa ccgcccgcat tatgggcgtt
      241 ggcctcaaca cgattttccg ccatttaaaa aactcaggcc gcagtcggta acctcgcgca
      301 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggatacgtcg
      361 gggctaaatc gcgccagcgc tggctgtttt acgcgtatga caggctccgg aagacggttg
      421 ttgcgcacgt attcggtgaa cgca
//
LOCUS       DAZXUL010000423          443 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZXUL010000423 DAZXUL010000000
VERSION     DAZXUL010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.434"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            213..>443
                     /locus_tag="ACH3L1_004781"
     CDS             213..>443
                     /locus_tag="ACH3L1_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFT4079143.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAZXUL010000424          438 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZXUL010000424 DAZXUL010000000
VERSION     DAZXUL010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.435"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>438
                     /locus_tag="ACH3L1_004782"
     CDS             <1..>438
                     /locus_tag="ACH3L1_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFT4079144.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYET"
BASE COUNT          113 a          103 c          129 g           93 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacg
//
LOCUS       DAZXUL010000425          434 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZXUL010000425 DAZXUL010000000
VERSION     DAZXUL010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.436"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            35..434
                     /locus_tag="ACH3L1_004783"
                     /pseudo
     CDS             35..434
                     /locus_tag="ACH3L1_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAZXUL010000426          433 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZXUL010000426 DAZXUL010000000
VERSION     DAZXUL010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.437"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..269
                     /locus_tag="ACH3L1_004784"
     CDS             <1..269
                     /locus_tag="ACH3L1_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFT4079145.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>433
                     /gene="tnpB"
                     /locus_tag="ACH3L1_004785"
     CDS             266..>433
                     /gene="tnpB"
                     /locus_tag="ACH3L1_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFT4079146.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKI"
BASE COUNT          109 a          113 c          122 g           89 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa ata
//
LOCUS       DAZXUL010000427          393 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZXUL010000427 DAZXUL010000000
VERSION     DAZXUL010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.439"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>393
                     /locus_tag="ACH3L1_004786"
     CDS             <1..>393
                     /locus_tag="ACH3L1_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFT4079147.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQT"
BASE COUNT           87 a          130 c          107 g           69 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatgagctt gctgagcaac ttatagaaca aac
//
LOCUS       DAZXUL010000428          392 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZXUL010000428 DAZXUL010000000
VERSION     DAZXUL010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.440"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..369
                     /locus_tag="ACH3L1_004787"
     rRNA            <1..369
                     /locus_tag="ACH3L1_004787"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          101 a           93 c          115 g           83 t
ORIGIN      
        1 ggaggaaggt ggggatgacg tcaagtcatc atggccctta cgaccagggc tacacacgtg
       61 ctacaatggc gcatacaaag agaagcgacc tcgcgagagc aagcggacct cataaagtgc
      121 gtcgtagtcc ggattggagt ctgcaactcg actccatgaa gtcggaatcg ctagtaatcg
      181 tggatcagaa tgccacggtg aatacgttcc cgggccttgt acacaccgcc cgtcacacca
      241 tgggagtggg ttgcaaaaga agtaggtagc ttaaccttcg ggagggcgct taccactttg
      301 tgattcatga ctggggtgaa gtcgtaacaa ggtaaccgta ggggaacctg cggttggatc
      361 acctccttac cttaaagaag cgttctttgt ag
//
LOCUS       DAZXUL010000429          388 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZXUL010000429 DAZXUL010000000
VERSION     DAZXUL010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.441"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(40..>388)
                     /locus_tag="ACH3L1_004788"
     CDS             complement(40..>388)
                     /locus_tag="ACH3L1_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFT4079148.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           89 c           87 g          120 t
ORIGIN      
        1 ttcgtgaagc tgttgaaggg tggattctta acggtggcgt taatcagaaa tgctcctgat
       61 gtgccagaac aatttatcag cgtgaagctc atacgctttg cgctgagcct ctatccagtc
      121 gtgtttattg tatacggaca ttatcccgcc aagttcatga ccgagcattc gctcagtaac
      181 atgaggctcg acattcatct cggacagccg tgtaacaagc gttcttctga aatcatgtgt
      241 tcgccagtaa cctagatcca tccctcccct tattctattt acgaatcggt tggctgcagc
      301 gatgctgatc ggtttgttta tgtcttctcc agggaaaaga acatcattgt acattgtcat
      361 ggccttttcg agaaaaggct taatttgt
//
LOCUS       DAZXUL010000430          382 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZXUL010000430 DAZXUL010000000
VERSION     DAZXUL010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.442"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..250)
                     /locus_tag="ACH3L1_004789"
                     /pseudo
     CDS             complement(1..250)
                     /locus_tag="ACH3L1_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           88 a          122 c           86 g           86 t
ORIGIN      
        1 cactgtctcc gttatggcgt acaccacatc ttccagtaat cgccgcttca ccggactcag
       61 gaccccgtat caccatccgg aaccccttat ccgccacatg ccaggagaac ctttccataa
      121 actcgtcgcc actcatcaca aggttttctt ctcgttttgt ccggtggctg ttgtaacgca
      181 ggccaatttc atcctgaccg gcataatgtt ccaggcggct catctgcacc ggctgcttct
      241 ccagccagtt aagccatcgg tttccctgct gaaaaatatc ggcaaaacgg ggaagcatca
      301 gaaaggcgga ataacgccat ccggccagtg aaccgtaccg cattcagggc aatacatgcc
      361 gtcggcgttg ataccggcag ga
//
LOCUS       DAZXUL010000431          375 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZXUL010000431 DAZXUL010000000
VERSION     DAZXUL010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.443"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            260..318
                     /locus_tag="ACH3L1_004790"
                     /pseudo
     CDS             260..318
                     /locus_tag="ACH3L1_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           99 a           83 c          105 g           88 t
ORIGIN      
        1 aaaaaaaatc agcgtcaaga aggtttcatt aacacatatc cctggcccaa aaagccgggg
       61 attttttata tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc
      121 gtgaccgctc ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc
      181 atcaacaatc ggggagtcag caaggggctg aaacgggaaa gcccctcccg aggaaggggc
      241 cttatagaag gaaagggtta tgatgaagtt cgtcatcata ctggttggtt gttactcgta
      301 agtttcgcag cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgtgc
      361 ctttacttta ggttg
//
LOCUS       DAZXUL010000432          375 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZXUL010000432 DAZXUL010000000
VERSION     DAZXUL010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.444"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>375
                     /locus_tag="ACH3L1_004791"
     CDS             <1..>375
                     /locus_tag="ACH3L1_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFT4079149.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAZXUL010000433          374 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZXUL010000433 DAZXUL010000000
VERSION     DAZXUL010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.445"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>374
                     /locus_tag="ACH3L1_004792"
     CDS             <1..>374
                     /locus_tag="ACH3L1_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFT4079150.1"
                     /translation="DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRS
                     LKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT           89 a           91 c          121 g           73 t
ORIGIN      
        1 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
       61 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
      121 aaagcgctgg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
      181 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
      241 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc
      301 aggagtctga agaacgaatg gatgccggtg gtgggttacg taagcttcag cgaggcagct
      361 cacgccataa cgga
//
LOCUS       DAZXUL010000434          372 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZXUL010000434 DAZXUL010000000
VERSION     DAZXUL010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.446"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..311)
                     /locus_tag="ACH3L1_004793"
     CDS             complement(<1..311)
                     /locus_tag="ACH3L1_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFT4079151.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAZXUL010000435          367 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZXUL010000435 DAZXUL010000000
VERSION     DAZXUL010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.447"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            166..367
                     /locus_tag="ACH3L1_004794"
                     /pseudo
     CDS             166..367
                     /locus_tag="ACH3L1_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          104 a           84 c           63 g          116 t
ORIGIN      
        1 tctaaatgag agtttttgat atataactat agacagctac tataactttc atctatttat
       61 tcacagcgcg aatgaaaaat aaaacactct gctttacagt gtttacttta taattaacag
      121 tcttatctca ctgttcaccc tgtaacgttc agagagcgtc ttcagatgtc tatttataaa
      181 attcctcttc cgctcaatat tctggaagct gcacgagagc gtattaccta gacactcaac
      241 accctacccc gcgtttgtgt ctcattttct ggcggcaaag actctggttt aatgctgcat
      301 ctgacagccg aacttgcccg acaaatgggc aaaaaaatct gcgttttgtt tatcaactgg
      361 gaggcgc
//
LOCUS       DAZXUL010000436          357 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZXUL010000436 DAZXUL010000000
VERSION     DAZXUL010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.448"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            39..>357
                     /locus_tag="ACH3L1_004795"
     CDS             39..>357
                     /locus_tag="ACH3L1_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFT4079152.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          112 a           86 c           91 g           68 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacc
//
LOCUS       DAZXUL010000437          344 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZXUL010000437 DAZXUL010000000
VERSION     DAZXUL010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.449"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>344
                     /locus_tag="ACH3L1_004796"
     CDS             <1..>344
                     /locus_tag="ACH3L1_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFT4079153.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAZXUL010000438          343 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZXUL010000438 DAZXUL010000000
VERSION     DAZXUL010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.450"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            51..>343
                     /locus_tag="ACH3L1_004797"
     CDS             51..>343
                     /locus_tag="ACH3L1_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFT4079154.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWS"
BASE COUNT           84 a           73 c          100 g           86 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg accttatctg gtc
//
LOCUS       DAZXUL010000439          341 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZXUL010000439 DAZXUL010000000
VERSION     DAZXUL010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.451"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..171
                     /gene="xisR"
                     /locus_tag="ACH3L1_004798"
     CDS             <1..171
                     /gene="xisR"
                     /locus_tag="ACH3L1_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFT4079155.1"
                     /translation="LMLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWY
                     NYPKINQFIKDS"
     gene            173..>341
                     /locus_tag="ACH3L1_004799"
     CDS             173..>341
                     /locus_tag="ACH3L1_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFT4079156.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           64 c           83 g           86 t
ORIGIN      
        1 ctgatgcttc gcactggtct gggtgccaga caaattgaag cataccgcca gaactgttgg
       61 gtggaaggct tccacttcaa acgagtatct cctttaggga agccagacag taagcgaggg
      121 attatctggt acaactatcc gaagataaat cagtttatca aagactcatg atatgtctaa
      181 attaccaaca ggtgtcgaga ttcgaggtaa atacattcgc atctggttca tgtttcgagg
      241 aaaacgatgt cgggaaacat tgaaaggctg ggagattact aacagtaaca ttaaaaaggc
      301 cgggaattta agagcgttga tagttcatca aatcagttcc g
//
LOCUS       DAZXUL010000440          338 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZXUL010000440 DAZXUL010000000
VERSION     DAZXUL010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.452"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>338
                     /locus_tag="ACH3L1_004800"
     CDS             <1..>338
                     /locus_tag="ACH3L1_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFT4079157.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLAD"
BASE COUNT           93 a           79 c           78 g           88 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcggatt
//
LOCUS       DAZXUL010000441          334 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZXUL010000441 DAZXUL010000000
VERSION     DAZXUL010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.453"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>334)
                     /locus_tag="ACH3L1_004801"
     CDS             complement(<1..>334)
                     /locus_tag="ACH3L1_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFT4079158.1"
                     /translation="RDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVI
                     RFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRAK
                     SLTFYGING"
BASE COUNT           75 a           95 c           87 g           77 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgg
//
LOCUS       DAZXUL010000442          333 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZXUL010000442 DAZXUL010000000
VERSION     DAZXUL010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.454"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(43..330)
                     /locus_tag="ACH3L1_004802"
     CDS             complement(43..330)
                     /locus_tag="ACH3L1_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079159.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           91 a           85 c           67 g           90 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgt
//
LOCUS       DAZXUL010000443          325 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZXUL010000443 DAZXUL010000000
VERSION     DAZXUL010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.denovo.455"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            103..>325
                     /locus_tag="ACH3L1_004803"
     CDS             103..>325
                     /locus_tag="ACH3L1_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFT4079160.1"
                     /translation="MIYSNQLRYTGKDGVYFHLQGQPDNFLSLSATENMEFDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTISISGVVVVG"
BASE COUNT          110 a           56 c           61 g           98 t
ORIGIN      
        1 aatttacgta gctcacgctt aacagagaaa atacttatat atcaaaggct tggttaaaaa
       61 tgattaaaat gcgctaagtc tttgaaatca aaagagaatt taatgattta ctctaatcag
      121 cttcgttaca ctggcaaaga tggtgtttac ttccatctac aaggccaacc agataatttc
      181 ttgtcactat cagcaactga aaacatggaa tttgatagcc ctatgatggt taccactcaa
      241 aatatgcaat cagggcagac tgtaacagat aatgtaatgc gttcacctag aactatttca
      301 atcagtggtg tggttgtggt tggtg
//
LOCUS       DAZXUL010000444         6391 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.guided.184, whole genome shotgun sequence.
ACCESSION   DAZXUL010000444 DAZXUL010000000
VERSION     DAZXUL010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.guided.184"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            510..749
                     /locus_tag="ACH3L1_004804"
     CDS             510..749
                     /locus_tag="ACH3L1_004804"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFT4079161.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(937..1914)
                     /gene="intI2"
                     /locus_tag="ACH3L1_004805"
                     /pseudo
     CDS             complement(937..1914)
                     /gene="intI2"
                     /locus_tag="ACH3L1_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2246..2719
                     /gene="dfrA1"
                     /locus_tag="ACH3L1_004806"
     CDS             2246..2719
                     /gene="dfrA1"
                     /locus_tag="ACH3L1_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFT4079162.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2814..3338
                     /gene="sat2"
                     /locus_tag="ACH3L1_004807"
     CDS             2814..3338
                     /gene="sat2"
                     /locus_tag="ACH3L1_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFT4079163.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3396..4231
                     /gene="aadA1"
                     /locus_tag="ACH3L1_004808"
                     /pseudo
     CDS             3396..4231
                     /gene="aadA1"
                     /locus_tag="ACH3L1_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            4260..4757
                     /locus_tag="ACH3L1_004809"
     CDS             4260..4757
                     /locus_tag="ACH3L1_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079164.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4818..5189
                     /locus_tag="ACH3L1_004810"
     CDS             4818..5189
                     /locus_tag="ACH3L1_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079165.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            5599..5976
                     /locus_tag="ACH3L1_004811"
     CDS             5599..5976
                     /locus_tag="ACH3L1_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079166.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5999..6151
                     /locus_tag="ACH3L1_004812"
                     /pseudo
     CDS             5999..6151
                     /locus_tag="ACH3L1_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(6207..>6391)
                     /locus_tag="ACH3L1_004813"
     CDS             complement(6207..>6391)
                     /locus_tag="ACH3L1_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFT4079167.1"
                     /translation="KIRRGVVKSSLNWPNSLFDQLYGQDGHRGVNHPKGLGELQVSRE
                     DMEGWAERVVREQFTH"
BASE COUNT         1758 a         1459 c         1499 g         1675 t
ORIGIN      
        1 tgtgggcgga caaaatagtt gggaactggg aggggtggaa atggagtttt taaggattat
       61 ttagggaaga gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct aatacgaaaa
      121 ttaaaaatga caaaatagtt tggaactaga tttcacttat ctggttaact aatctagcta
      181 acctgccgct cagcttagta cgatcctata tccaactgat cataatcctt tcatcagtat
      241 cccaaattaa atccccctcc tagactgcct caccaacact ttacagtgat cccgtatttc
      301 cggacaggtg attgattttt ttcgcttgta caggtgttaa gccacagtta tcttgatgtg
      361 gacattatgc attgtaatag cccattaaca tgcgctaaga gcataaatta atatacagta
      421 acaagcatta cgaggtgcta atgtttggaa agcagcctct tccaactaca ataataagtt
      481 aactatcctc cacccacaaa taattacaaa tgaccccttt ccgctataac tccgatctca
      541 ccagcggctc gctacaaacc cgagagtgtc gcattattac tggcttgtta ctccaagagc
      601 ttgatgaagc tgcctgggat aaagccatgt ataaggaaaa tgtgcttcag aaacgcacgc
      661 aatctacggt aagacgtatt tcttcagcgc ttagaaaacg cctagaacac ctaagctctg
      721 acttttgggc ttttgcgttt ttatgttagg cattgtctgg caactggagg aagtgtaatg
      781 accatgaatg gtcactgtga ttgacagtat cttgcttaaa cttatctgaa caatggtaga
      841 actggctcat atttatcccc tctatgattg tttatctttt tcaccataaa aagtatcagg
      901 ggctactcag ggctacataa agatatttat ctacttttac tgattgataa gtagcatcag
      961 tccatccgca ggactggtgg tgccggcaaa atgctgaccc aacacatgcg tatagatttg
     1021 cgtggtctta acatcgttat gccctaagag ttcttgcaca gtgcgaatat cacgccccgc
     1081 ttgtaataga tgcgtagcaa acgagtgacg aaatgtatga caagtgacac gcttgctaac
     1141 gatgcctgct ttttgtacgg ctgccttcaa tgcctttcgc gcaacggagt catgcagatg
     1201 atggcggcat aatttgccgt tatacgggtg gttgcagagc gtgctggagg gaaagacaaa
     1261 catccacgcc gcttgtcgat aagcagaagg gtatttgtga tctaaagcaa aaggcagcga
     1321 tggccctacg ccttgtaagt tgtcgtcttg ctgaataagc cgcgcttgct caatgagtta
     1381 ttttattgct gggattaggc gcgtgggcag taggctgttt ctgcttttcc cacccttacc
     1441 gtcatgcaca gtgatgcagc cattatcaaa atcaaaatct ttaacccgca aacgcaagca
     1501 ttcattaatg cgcaaacctg caccatacag cagcgtaaaa ataacttggt tgcgagtatc
     1561 cataacctgc aaaatgcgtt gcacttcatt tgcagagata acagagggta gccgtctagg
     1621 cttgcttgca gggatataat caatatcgcc caacggctgt tgtaaaaacc tgttgtacaa
     1681 aaaagctagg gcatttaaag cgattttctg cgtgtttatg gctacatgtc tgctgtttgc
     1741 taagctggat aaaaacagcc tgacctcttc actgcccatg gtctgaggat gacgtttttt
     1801 gtgaaacaga ataaaacgct taatccagtg caggtaagtt ttttcagttt tcagcgcata
     1861 acctttttgt cgcatatccg tgcgtataga atttaaaaat ggactgttag acataaaacg
     1921 ctccttgtct tgcaactgtc tgcctataca gcctattcta gctgggattt aaaaaagtgc
     1981 ctgtttttta cgcctagaga tgcttgttta ccggtagagt tttaatttaa tgctaaataa
     2041 attaaaatgt tatgagttct ttgggtgaga taatgtgcat cgtgcaagca ggatagacgg
     2101 catgcacgat ttgtaataac agagtgtctt gtatttttaa agaaagtcta tttaatacaa
     2161 gtgattatat taattaacgg taagcatcag cgggtgacaa aacgagcatg cttactaata
     2221 aaatgttaac ctctgaggaa gaattgtgaa actatcacta atggtagcta tatcgaagaa
     2281 tggagttatc gggaatggcc ctgatattcc atggagtgcc aaaggtgaac agctcctgtt
     2341 taaagctatt acctataacc aatggctgtt ggttggacgc aagacttttg aatcaatggg
     2401 agcattaccc aaccgaaagt atgcggtcgt aacacgttca agttttacat ctgacaatga
     2461 gaacgtattg atctttccat caattaaaga tgctttaacc aacctaaaga aaataacgga
     2521 tcatgtcatt gtttcaggtg gtggggagat atacaaaagc ctgatcgatc aagtagatac
     2581 actacatata tctacaatag acatcgagcc ggaaggtgat gtttactttc ctgaaatccc
     2641 cagcaatttt aggccagttt ttacccaaga cttcgcctct aacataaatt atagttacca
     2701 aatctggcaa aagggttaac aagtggcagc aacggattcg caaacctgtc acgccttttg
     2761 taccaaaagc cgcgccaggt ttgcgatccg ctgtgccagg cgttaggcgt catatgaaga
     2821 tttcggtgat ccctgagcag gtggcggaaa cattggatgc tgagaaccat ttcattgttc
     2881 gtgaagtgtt cgatgtgcac ctatccgacc aaggctttga actatctacc agaagtgtga
     2941 gcccctaccg gaaggattac atctcggatg atgactctga tgaagactct gcttgctatg
     3001 gcgcattcat cgaccaagag cttgtcggga agattgaact caactcaaca tggaacgatc
     3061 tagcctctat cgaacacatt gttgtgtcgc acacgcaccg aggcaaagga gtcgcgcaca
     3121 gtctcatcga atttgcgaaa aagtgggcac taagcagaca gctccttggc atacgattag
     3181 agacacaaac gaacaatgta cctgcctgca atttgtacgc aaaatgtggc tttactctcg
     3241 gcggcattga cctgttcacg tataaaacta gacctcaagt ctcgaacgaa acagcgatgt
     3301 actggtactg gttctcggga gcacaggatg acgcctaaca attcattcaa gccgacaccg
     3361 cttcgcggcg cggcttaatt caggagttaa acatcatgag ggaagcggtg atcgccgaag
     3421 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     3481 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     3541 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     3601 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     3661 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     3721 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     3781 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     3841 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     3901 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     3961 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     4021 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctaggc
     4081 aggcttatct tggacaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     4141 ttcactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta acaattcgtt
     4201 caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagagagctg gggaagacta
     4261 tgcgcgatct gttgaaggtg gttctaagcc tcgtacttgc gatggcatcg gggcaggcac
     4321 ttgctgacct gccaattgtt ttagtggatg aagctcgtct tccctatgac tactccccat
     4381 ccaactacga catttctcca agcaactacg acaactccat aagcaattac gacaatagtc
     4441 catcaaatta cgacaactct gagagcaact acgataatag ttcatccaat tacgacaata
     4501 gtcgcaacgg aaatcgtagg cttatatata gcgcaaatgg gtctcgcact ttcgccggct
     4561 actacgtcat tgccaacaat gggacaacga acttcttttc cacatctggc aaaaggatgt
     4621 tctacacccc aaaagggggg cgcggcgtct atggcggcaa agatgggagc ttctgcgggg
     4681 cattggtcgt cataaatggc caattttcgc ttgccctgac agataacggc ctgaagatca
     4741 tgtatctaag caactagcct gctctctaat aaaatgttag gcctcaacat ctagtcgcaa
     4801 gctgagggga accactagtg tcatacgaac ctccaagaga cggttacaca aacgggtaca
     4861 ttgttgatgt catgtatgac aatcgcccaa gtaagtatcc agctgtgttc agaacgtacg
     4921 tccgaattcc agactatgtc gaactagata acgcggagga gctggccgac gcaatcattc
     4981 gagaggttga gttaaaggag gcatggctca caagggtctt ggggaactca gccgatcctg
     5041 acgcttctgt gggcaagtgg gaccggcgcc tagcgcagtg cggtggtatt gatgagatgt
     5101 caagtggggt gcaggcaaca tacttcagtc caaacggtca gctggtcggc ttagtggcat
     5161 acattgatgc cgaatggatg gatgaatagc tctgacgtta cgattcaggt gagacaacca
     5221 gatgggcatt gcgtacaagc tcagttgcca ccgatgtggc tacgagtctg atcttcttta
     5281 cctaggccaa ggtatggcga tgcttcctga gcaaatagtc ggaacgtgca catgctgcga
     5341 caatctaacg acgatttcta taaatgaaag ctacggagta tgctcacttt gtggggctaa
     5401 ggatcgtgtg atatttacaa gctatcagca tgaggcaagg ccaagaaatc cctgctatct
     5461 agagagaagt ttcaaatatc aatgcccaag atgccatgcc ttcagtatgg agcctccgga
     5521 tctaccggag atgctttggg actaatgctc cgctttgcct cgggtatccg ttcgacctat
     5581 gtttaagagg aaacgcacgt gtctgccgcc aaagctcttg cctatgcact ttgtgacaag
     5641 ttggaaagca aaaaagttga tgaagtcgta cgaaatcttg tttctagcgg ctggaatatt
     5701 agggaagtgg cctgggaagc ttcgtccttt gatcatgaaa tgccctactt tgacacgggg
     5761 cgggacttcg atattggatt tcctgacacg ctgtcatcca agattgagtt tttgaccgcg
     5821 aatggatcaa tcgtccaagc acgtgctcgc ctgcacttca agcaggctct aatttttgcc
     5881 aaggcgtcca aacatgtcgc cgcgctcaag aactcattgg attactacta tggatcgtct
     5941 gtaatccccc ctcaaaacca cggaggctat ttgtagaaaa ttccattaat atgaactcaa
     6001 ggtgttccct acggacgaat cagcgatgaa ggtggtgtac ctggctatcc aggcggcgac
     6061 caagaaatgg acgatgccga tccgcaactg gaagccggcc atgaaccgtt ttatgatcga
     6121 atttggtgac cgtttaaatg gccaccttta actgaccggc aaaagcactt acacaaaagg
     6181 gtttacaggc tctttcagtc attcctttaa tgcgtaaatt gctctctaac cactctctca
     6241 gcccaccctt ccatatcctc tctcgatact tgcagctccc ccaacccctt tggatgattc
     6301 acccctctat gcccgtcttg tccatataat tgatcaaaca aactatttgg ccaattcaat
     6361 gagctcttca ccactccacg ccgtatcttt t
//
LOCUS       DAZXUL010000445         2352 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.guided.283, whole genome shotgun sequence.
ACCESSION   DAZXUL010000445 DAZXUL010000000
VERSION     DAZXUL010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.guided.283"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            817..1632
                     /gene="sul2"
                     /locus_tag="ACH3L1_004814"
     CDS             817..1632
                     /gene="sul2"
                     /locus_tag="ACH3L1_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFT4079168.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1719..2021
                     /locus_tag="ACH3L1_004815"
     CDS             1719..2021
                     /locus_tag="ACH3L1_004815"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079169.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
BASE COUNT          547 a          639 c          596 g          570 t
ORIGIN      
        1 atctcctccg ctcaccatag taagcggagg attcaggatt tcaatccatc accagaggta
       61 caataaatgt gtattagtag cccggaaaca aactcatggt ctgttattta tcgtaaaaat
      121 tccggagaag atatcaatat cacttccctg acgtttaaaa atagtcttct ggcagcccgg
      181 acactgatgg tgcccgaaaa ctatatgata tgcatactca gaaacgggga gagggtcaga
      241 cgatgggaca gagaaatact tgcaggctca aacagatggt acaagtgttc tcccgataat
      301 ttcgaaatac tgggtaagct ccccataata aataaagtca caaaactgat aaaatcataa
      361 tctacggatt ttaatcagtt aagctaaatc aggatgaacg ctttgattat gaattataca
      421 tgaaaacaaa tatgggcagc acaaaaaatg gtgccagggt tccccacgtt cgtaatagat
      481 tcacttctat ggatattggt atgaattccc agatcccact ctattgtaaa caagacattt
      541 ttatctttta tattcaatgg cttattttcc tgctaattgg taataccatg aaaaatacca
      601 tgctcagaaa aggcttaaca atattttgaa aaattgccta ctgagcgctg ccgcacagct
      661 ccataggccg ctttcctggc tttgcttcca gatgtatgct attctgctcc tgcagctaat
      721 ggatcaccgc aaacaggtta ctcgcctggg gattcccttt cgacccgagc atccgtatga
      781 gactcatgct cgattattat tattatagaa gcccccatga ataaatcgct catcattttc
      841 ggcatcgtca acataacctc ggacagtttc tccgatggag gccggtatct ggcgccagac
      901 gcagccattg cgcaggcgcg taagctgatg gccgaggggg cagatgtgat cgacctcggt
      961 ccggcatcca gcaatcccga cgccgcgcct gtttcgtccg acacagaaat cgcgcgtatc
     1021 gcgccggtgc tggacgcgct caaggcagat ggcattcccg tctcgctcga cagttatcaa
     1081 cccgcgacgc aagcctatgc cttgtcgcgt ggtgtggcct atctcaatga tattcgcggt
     1141 tttccagacg ctgcgttcta tccgcaattg gcgaaatcat ctgccaaact cgtcgttatg
     1201 cattcggtgc aagacgggca ggcagatcgg cgcgaggcac ccgctggcga catcatggat
     1261 cacattgcgg cgttctttga cgcgcgcatc gcggcgctga cgggtgccgg tatcaaacgc
     1321 aaccgccttg tccttgatcc cggcatgggg ttttttctgg gggctgctcc cgaaacctcg
     1381 ctctcggtgc tggcgcggtt cgatgaattg cggctgcgct tcgatttgcc ggtgcttctg
     1441 tctgtttcgc gcaaatcctt tctgcgcgcg ctcacaggcc gtggtccggg ggatgtcggg
     1501 gccgcgacac tcgctgcaga gcttgccgcc gccgcaggtg gagctgactt catccgcaca
     1561 cacgagccgc gccccttgcg cgacgggctg gcggtattgg cggcgctgaa agaaaccgca
     1621 agaattcgtt aactgcacat tcgggatatt tctctatatt cgcggttcag caggcatgtc
     1681 ccctttgagg gcgacccgac gacaggataa tcgaccttat ggtgcgcaaa tatttcggca
     1741 cagacggtat tcgtggcaaa gccaacgaag gcgcgatgac ggcggaaacc gccttgcgcg
     1801 tcggcatggc ggctggccgt gtctttcgtc gcggtgacca ccgccatcgt gtcgtgatcg
     1861 gcaaggatac gcgcctgtcg ggctatatgc ttgaacccgc gctcacagcc ggtttcacct
     1921 cgatgggcat ggacgtattc ctttttggcc cgctgccgac aacgtatagg aagaataaac
     1981 gcccttttca cccaagtcca acagctttgg accgcagttg actctttcga cacccctgcg
     2041 atgcaaccca atccggctga cggggagcca gcaacgctga aaatttaccc tcctctttcc
     2101 cactagcggc tccttttccg acaaccagca cggcggatcc ctgccgcggc gctgtgaacg
     2161 cagcattttg attggtatcg ttggccttca ggctcgtcag tcaaacagac ccaggagcag
     2221 ctcagccggt ggcgcccggc tttcgggtaa cgccctggtc ccgctggttt cggctttgtg
     2281 cttcaggtga tcaaggatct gcttgatcac tatgtgggcg gacaataaag tcttaaactg
     2341 aacaaaatag at
//
LOCUS       DAZXUL010000446         1820 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.guided.320, whole genome shotgun sequence.
ACCESSION   DAZXUL010000446 DAZXUL010000000
VERSION     DAZXUL010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.guided.320"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            68..772
                     /locus_tag="ACH3L1_004816"
     CDS             68..772
                     /locus_tag="ACH3L1_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFT4079170.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYISSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            894..1799
                     /locus_tag="ACH3L1_004817"
     CDS             894..1799
                     /locus_tag="ACH3L1_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFT4079171.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT          379 a          528 c          541 g          372 t
ORIGIN      
        1 gatgggcact gttgcaaata gtcggtggtg ataaacttat catccccttt tgctgatgga
       61 gctgcacatg aacccattca aaggccggca ttttcagcgt gacatcattc tgtgggccgt
      121 acgctggtac tgcaaatacg gcatcagtta ccgtgagctg caggagatgc tggctgaacg
      181 cggagtgaat gtcgatcact ccacgattta ccgctgggtt cagcgttatg cgcctgaaat
      241 ggaaaaacgg ctgcgctggt actggcgtaa cccttccgat ctttgcccgt ggcacatgga
      301 tgaaacctac gtgaaggtca atggccgctg ggcgtatctg taccgggccg tcgacagccg
      361 gggccgcact gtcgattttt atatctcctc ccgtcgtaac agcaaagctg cataccggtt
      421 tctgggtaaa atcctcaaca acgtgaagaa gtggcagatc ccgcgattca tcaacacgga
      481 taaagcgccc gcctatggtc gcgcgcttgc tctgctcaaa cgcgaaggcc ggtgcccgtc
      541 tgacgttgaa caccgacaga ttaagtaccg gaacaacgtg attgaatgcg atcatggcaa
      601 actgaaacgg ataatcggcg ccacgctggg atttaaatcc atgaagacgg cttacgccac
      661 catcaaaggt attgaggtga tgcgtgcact acgcaaaggc caggcctcag cattttatta
      721 tggtgatccc ctgggcgaaa tgcgcctggt aagcagagtt tttgaaatgt aaggcctttg
      781 aataagacaa aaggctgcct catcgctaac tttgcaacag tgccggattg aatataaccg
      841 acgtgactgt tatatttagg tggctaaacc cgtcaagccc tcaggagtga atcatgaccg
      901 tagtcacgac cgccgatacc tcccaactgt acgcacttgc agcccgacat gggctcaagc
      961 tccatggccc gctgactgtc aatgagcttg ggctcgacta taggatcgtg atcgccaccg
     1021 tcgacgatgg acgtcggtgg gtgctgcgca tcccgcgccg agccgaggta agcgcgaagg
     1081 tcgaaccaga ggcgcgggtg ctggcaatgc tcaagaatcg cctgccgttc gcggtgccgg
     1141 actggcgcgt ggccaacgcc gagctcgttg cctatcccat gctcgaagac tcgactgcga
     1201 tggtcatcca gcctggttcg tccacgcccg actgggtcgt gccgcaggac tcggaggtct
     1261 tcgcggagag cttcgcgacc gcgctcgccg ccctgcatgc cgtccccatt tccgccgccg
     1321 tggatgcggg gatgctcatc cgtacaccga cgcaggcccg tcagaaggtg gccgacgacg
     1381 ttgaccgcgt ccgacgcgag ttcgtggtga acgacaagcg cctccaccgg tggcagcgct
     1441 ggctcgacga cgattcgtcg tggccagatt tctccgtggt ggtgcatggc gatctctacg
     1501 tgggccatgt gctcatcgac aacacggagc gcgtcagcgg gatgatcgac tggagcgagg
     1561 cccgcgttga tgaccctgcc atcgacatgg ccgcgcacct tatggtcttt ggtgaagagg
     1621 ggctcgcgaa gctcctcctc acgtatgaag cggccggtgg ccgggtgtgg ccgcggctcg
     1681 cccaccacat cgcggagcgc cttgcgttcg gggcggtcac ctacgcactc ttcgccctcg
     1741 actcgggtaa cgaagagtac ctcgctgcgg cgaaggcgca gctcgccgca gcggaatgag
     1801 cgaacgtcga tatagcccgc
//
LOCUS       DAZXUL010000447         1695 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.guided.327, whole genome shotgun sequence.
ACCESSION   DAZXUL010000447 DAZXUL010000000
VERSION     DAZXUL010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.guided.327"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            23..862
                     /gene="sul1"
                     /locus_tag="ACH3L1_004818"
     CDS             23..862
                     /gene="sul1"
                     /locus_tag="ACH3L1_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFT4079172.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            990..1193
                     /locus_tag="ACH3L1_004819"
     CDS             990..1193
                     /locus_tag="ACH3L1_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079173.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            1349..>1695
                     /locus_tag="ACH3L1_004820"
     CDS             1349..>1695
                     /locus_tag="ACH3L1_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFT4079174.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQG"
BASE COUNT          318 a          498 c          512 g          367 t
ORIGIN      
        1 agtcgctgcg gaggccgacg ccatggtgac ggtgttcggc attctgaatc tcaccgagga
       61 ctccttcttc gatgagagcc ggcggctaga ccccgccggc gctgtcaccg cggcgatcga
      121 aatgctgcga gtcggatcag acgtcgtgga tgtcggaccg gccgccagcc atccggacgc
      181 gaggcctgta tcgccggccg atgagatcag acgtattgcg ccgctcttag acgccctgtc
      241 cgatcagatg caccgtgttt caatcgacag cttccaaccg gaaacccagc gctatgcgct
      301 caagcgcggc gtgggctacc tgaacgatat ccaaggattt cctgaccctg cgctctatcc
      361 cgatattgct gaggcggact gcaggctggt ggttatgcac tcagcgcagc gggatggcat
      421 cgccacccgc accggtcacc ttcgacccga agacgcgctc gacgagattg tgcggttctt
      481 cgaggcgcgg gtttccgcct tgcgacggag cggggtcgct gccgaccggc tcatcctcga
      541 tccggggatg ggatttttct tgagccccgc accggaaaca tcgctgcacg tgctgtcgaa
      601 ccttcaaaag ctgaagtcgg cgttggggct tccgctattg gtctcggtgt cgcggaaatc
      661 cttcttgggc gccaccgttg gccttcctgt aaaggatctg ggtccagcga gccttgcggc
      721 ggaacttcac gcgatcggca atggcgctga ctacgtccgc acccacgcgc ctggagatct
      781 gcgaagcgca atcaccttct cggaaaccct cgcgaaattt cgcagtcgcg acgccagaga
      841 ccgagggtta gatcatgcct agcattcacc ttccggccgc ccgctagcgg accctggtca
      901 ggttccgcga aggtgggcgc agacatgctg ggctcgtcag gatcaaactg cactatgagg
      961 cggcggttca taccgcgcca ggggagcgaa tggacagcga ggagcctccg aacgttcggg
     1021 tcgcctgctc gggtgatatc gacgaggttg tgcggctgat gcacgacgct gcggcgtgga
     1081 tgtccgccaa gggaacgccc gcctgggacg tcgcgcggat cgaccggaca ttcgcggaga
     1141 ccttcgtcct gagatccgag ctcctaggga tcgcctcaga aaacggaaaa taaagcacgc
     1201 taagccgtaa gtaagcgtgc tcctgtgaaa gccacagcta aaactgcgta gtacacatag
     1261 aggtattttt ttctagattt atatcgaatt tcgttatcga atttcgctta ttattgatca
     1321 actcgttgca gatcatgaag gcgaaagtat gacgaataac cctaccgatg acagcagacc
     1381 atggtcggtc tttcttattt ttctgcggct tggattgaca tcttttggcg gccccattgc
     1441 gcacttgggc tacttccgcg ccgaatttgt cacacggcgg cgctggctct ccgaacggag
     1501 ctatgctgac ttggtcgcgc tttgtcagtt cttgccaggg cctgcaagca gccaggtcgg
     1561 catagcggta ggactgtctc gggctggata cagcggggcg ctggctgctt gggctggctt
     1621 cacgctgccg tctgccatag ccttgatcct ttttgcgctc ggcatctcca gctatggcga
     1681 ttacgtctcg caggg
//
LOCUS       DAZXUL010000448         1658 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.guided.330, whole genome shotgun sequence.
ACCESSION   DAZXUL010000448 DAZXUL010000000
VERSION     DAZXUL010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.guided.330"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..649)
                     /locus_tag="ACH3L1_004821"
     CDS             complement(<1..649)
                     /locus_tag="ACH3L1_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFT4079175.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHS"
     gene            911..1369
                     /gene="gpt"
                     /locus_tag="ACH3L1_004822"
     CDS             911..1369
                     /gene="gpt"
                     /locus_tag="ACH3L1_004822"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFT4079176.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1461..>1658
                     /locus_tag="ACH3L1_004823"
     CDS             1461..>1658
                     /locus_tag="ACH3L1_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFT4079177.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREI"
BASE COUNT          408 a          426 c          406 g          418 t
ORIGIN      
        1 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
       61 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt
      121 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
      181 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
      241 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc
      301 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
      361 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
      421 ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca ggttgacgaa
      481 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
      541 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
      601 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
      661 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
      721 acaagacagg cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg
      781 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
      841 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
      901 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
      961 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     1021 tggcggtctg gtaccggggg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     1081 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     1141 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     1201 tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     1261 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca
     1321 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     1381 cctggcattg ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa
     1441 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     1501 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     1561 ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     1621 tgatgtggat ctggcgcggt attgacccac gtgaaatc
//
LOCUS       DAZXUL010000449         1528 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.guided.338, whole genome shotgun sequence.
ACCESSION   DAZXUL010000449 DAZXUL010000000
VERSION     DAZXUL010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.guided.338"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            111..1316
                     /gene="tet(B)"
                     /locus_tag="ACH3L1_004824"
     CDS             111..1316
                     /gene="tet(B)"
                     /locus_tag="ACH3L1_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFT4079178.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1429..>1528)
                     /locus_tag="ACH3L1_004825"
     CDS             complement(1429..>1528)
                     /locus_tag="ACH3L1_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFT4079179.1"
                     /translation="IAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          374 a          283 c          342 g          529 t
ORIGIN      
        1 atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt gacactctat
       61 cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa atgaatagtt
      121 cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc cttatcatgc
      181 cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct aaccactttg
      241 gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg cttggaaaaa
      301 tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc gcatcgctgg
      361 attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc cgtttgcttt
      421 cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat accacctcag
      481 cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt ggtttaatag
      541 cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc ttttttatcg
      601 ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt gaaaccaaaa
      661 atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat tcggtataca
      721 tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg caattgatag
      781 gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga tggaatagca
      841 tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc caagcctttg
      901 tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc ggatttattg
      961 cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta gttttccctg
     1021 ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga gtgatgtcta
     1081 tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc cttaccaatg
     1141 caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca ctaccaattt
     1201 gggatggctg gatttggatt attggtttag cgttttactg tattattatc ctgctatcga
     1261 tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt gcttagttat
     1321 ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa cccaagaggg
     1381 cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt atttatcttt
     1441 caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat cggcaagctc
     1501 ttttaggttt ttcgcatgta ttgcgata
//
LOCUS       DAZXUL010000450         1419 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.guided.345, whole genome shotgun sequence.
ACCESSION   DAZXUL010000450 DAZXUL010000000
VERSION     DAZXUL010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.guided.345"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..1208
                     /locus_tag="ACH3L1_004826"
     CDS             75..1208
                     /locus_tag="ACH3L1_004826"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFT4079180.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1297..>1419
                     /locus_tag="ACH3L1_004827"
     CDS             1297..>1419
                     /locus_tag="ACH3L1_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFT4079181.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLG"
BASE COUNT          363 a          372 c          344 g          340 t
ORIGIN      
        1 gttacaatct aacgcatcgc caatgtaaat ccggcccgcc tatggcgggc cgttttgtat
       61 ggaaaccaga ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttact
      121 ccacatttgc tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta
      181 tagagcaaca aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt
      241 actttacctg gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt
      301 ttgagttagg ttcggtcagc aaaacattta cgggcgtgct tggtggcgac gctattgctc
      361 gaggggaaat caagttaagc gatcccgcaa caaaatactg gcctgaactt accgctaaac
      421 agtggaatgg gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc
      481 aggtgccgga tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc
      541 ctgcatgggc tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg
      601 cactggctgt gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc
      661 agccactcaa actcaaccat acgtggatta atgtacctcc cccagaagaa aagaattacg
      721 cctggggata tcgcgaaggt aaggcagtgc atgtttcgcc aggggcgtta gatgctgaag
      781 cttatggtgt gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttacccc
      841 cccttgatat caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact
      901 ggcaaaccgg cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtcaatc
      961 ctgacagcat cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag
     1021 cgattaagcc cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg
     1081 gcggatttgg tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg
     1141 ctaacaaaaa ctatcccaat ccagcgagag tcgccgccgc ctgtcagatt ctcaacgctc
     1201 tacagtaaaa tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc
     1261 atctacactt agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc
     1321 gcagcgacag ctgcatttct ggtcgttgcc tgtagttttc ctacgccgcc gcgtggcgtg
     1381 accgtagtga ataatttcga cgccaaacgt tatctcggt
//
LOCUS       DAZXUL010000451         1269 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.guided.357, whole genome shotgun sequence.
ACCESSION   DAZXUL010000451 DAZXUL010000000
VERSION     DAZXUL010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.guided.357"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            88..378
                     /locus_tag="ACH3L1_004828"
     CDS             88..378
                     /locus_tag="ACH3L1_004828"
                     /inference="COORDINATES: protein motif:HMM:NF017990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1010 domain-containing protein"
                     /protein_id="HFT4079182.1"
                     /translation="MFIQTAFSFSGVIQCLFCLFSGLRLHGLRRFSVFLASSPCVASA
                     SSYRFCSAVPPRWRSVFSRLAPVAKFKLSVLASGSNISVKPTRILRSAYLAR"
     gene            384..1222
                     /locus_tag="ACH3L1_004829"
                     /pseudo
     CDS             384..1222
                     /locus_tag="ACH3L1_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
BASE COUNT          279 a          332 c          330 g          328 t
ORIGIN      
        1 atgctgcgca ttttggttcc ctccgctgcg ctccggctct cgttacgtcc aacgttagca
       61 ccactgaaac ccagctttat ttagctcatg tttattcaaa cggcatttag cttttcaggc
      121 gttattcagt gcctgttttg ccttttttcc gggcttcgcc tgcatgggct gcgcaggttt
      181 tcagtctttt tggcctctag cccttgcgta gcaagcgcaa gcagctatcg tttttgcagt
      241 gctgtgccgc ctcggtggcg cagcgttttt tcacggttag cgcccgtcgc caaattcaag
      301 ttatccgttt tggcttctgg ttctaacatt tcggtcaagc cgacccgcat tctgcggtcg
      361 gcttacctcg cccgttagac atcatgaggg aagcggtgac catcgaaatt tcgaaccaac
      421 tatcagaggt gctaagcgtc attgagcgcc atctggaatc aacgttgctg gccgtgcatt
      481 tgtacggctc cgcagtggat ggcggcctga agccatacag cgatattgat ttgttggtta
      541 ctgtggccgt aaagcttgat gaaacgacgc ggcgagcatt gctcaatgat cttatggagg
      601 cttcggcttt ccctggcgag agcgagacgc tccgcgctat agaagtcacc cttgtcgtgc
      661 atgacgacat catcccgtgg cgttatccgg ctaagcgcga gctgcaattt ggagaatggc
      721 agcgcaatga cattcttgcg ggtatcttcg agccagccat gatcgacatt gatctagcta
      781 tcctgcttac aaaagcaaga gaacatagcg ttgccttggt aggtccggca gcggaggaat
      841 tctttgaccc ggttcctgaa caggatctat tcgaggcgct gagggaaacc ttgaagctat
      901 ggaactcgca gcccgactgg gccggcgatg agcgaaatgt agtgcttacg ttgtcccgca
      961 tttggtacag cgcaataacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     1021 taaaacgcct acctgcccag tatcagcccg tcttacttga agctaagcaa gcttatctgg
     1081 gacaaaaaga agatcacttg gcctcacgcg cagatcactt ggaagaattt attcgctttg
     1141 tgaaaggcga gatcatcaag tcagttggta aatgatgtct aacaattcgt tcaagccgac
     1201 cgcgctacgc gcggcggctt aactccggcg ttagatgcac taagcacata attgctcaca
     1261 gccaaacta
//
LOCUS       DAZXUL010000452         1087 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.guided.370, whole genome shotgun sequence.
ACCESSION   DAZXUL010000452 DAZXUL010000000
VERSION     DAZXUL010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.guided.370"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            133..792
                     /locus_tag="ACH3L1_004830"
     CDS             133..792
                     /locus_tag="ACH3L1_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFT4079183.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          289 a          230 c          257 g          311 t
ORIGIN      
        1 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
       61 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
      121 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      181 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      241 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      301 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
      361 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      421 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      481 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      541 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      601 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      661 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
      721 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
      781 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc
      841 tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc gacccggtcg
      901 tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt accggtttat
      961 tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag tttgctcagg
     1021 ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc cagagcctga
     1081 taaaaac
//
LOCUS       DAZXUL010000453          824 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.guided.391, whole genome shotgun sequence.
ACCESSION   DAZXUL010000453 DAZXUL010000000
VERSION     DAZXUL010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.guided.391"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..750
                     /gene="blaOXA"
                     /locus_tag="ACH3L1_004831"
     CDS             <1..750
                     /gene="blaOXA"
                     /locus_tag="ACH3L1_004831"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-1 family class D beta-lactamase"
                     /protein_id="HFT4079184.1"
                     /translation="DISTVASPLFEGTEGCFLLYDASTNAEIAQFNKAKCATQMAPDS
                     TFKIALSLMAFDAEIIDQKTIFKWDKTPKGMEIWNSNHTPKTWMQFSVVWVSQEITQK
                     IGLNKIKNYLKDFDYGNQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNL
                     PVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVF
                     VSALTGNLGSNLTSSIKAKKNAITILNTLNL"
BASE COUNT          316 a          147 c          145 g          216 t
ORIGIN      
        1 gatatctcta ctgttgcatc tccattattt gaaggaactg aaggttgttt tttactttac
       61 gatgcatcca caaacgctga aattgctcaa ttcaataaag caaagtgtgc aacgcaaatg
      121 gcaccagatt caactttcaa gatcgcatta tcacttatgg catttgatgc ggaaataata
      181 gatcagaaaa ccatattcaa atgggataaa acccccaaag gaatggagat ctggaacagc
      241 aatcatacac caaagacgtg gatgcaattt tctgttgttt gggtttcgca agaaataacc
      301 caaaaaattg gattaaataa aatcaagaat tatctcaaag attttgatta tggaaatcaa
      361 gacttctctg gagataaaga aagaaacaac ggattaacag aagcatggct cgaaagtagc
      421 ttaaaaattt caccagaaga acaaattcaa ttcctgcgta aaattattaa tcacaatctc
      481 ccagttaaaa actcagccat agaaaacacc atagagaaca tgtatctaca agatctggat
      541 aatagtacaa aactgtatgg gaaaactggt gcaggattca cagcaaatag aaccttacaa
      601 aacggatggt ttgaagggtt tattataagc aaatcaggac ataaatatgt ttttgtgtcc
      661 gcacttacag gaaacttggg gtcgaattta acatcaagca taaaagccaa gaaaaatgcg
      721 atcaccattc taaacacact aaatttataa aaaatctaat ggcaaaatcg cccaaccctt
      781 caatggtgat gctgccaact tactgattta gtgtatgatg gtgt
//
LOCUS       DAZXUL010000454          643 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.guided.413, whole genome shotgun sequence.
ACCESSION   DAZXUL010000454 DAZXUL010000000
VERSION     DAZXUL010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.guided.413"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            123..620
                     /gene="dfrA12"
                     /locus_tag="ACH3L1_004832"
     CDS             123..620
                     /gene="dfrA12"
                     /locus_tag="ACH3L1_004832"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /protein_id="HFT4079185.1"
                     /translation="MNSESVRIYLVAAMGANRVIGNGPNIPWKIPGEQKIFRRLTEGK
                     VVVMGRKTFESIGKPLPNRHTLVISRQANYRATGCVVVSTLSHAIALASELGNELYVA
                     GGAEIYTLALPHAHGVFLSEVHQTFEGDAFFPMLNETEFELVSTETIQAVIPYTHSVY
                     ARRNG"
BASE COUNT          159 a          177 c          157 g          150 t
ORIGIN      
        1 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
       61 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttagcc
      121 atatgaactc ggaatcagta cgcatttatc tcgttgctgc gatgggagcc aatcgggtta
      181 ttggcaatgg tcctaatatc ccctggaaaa ttccgggtga gcagaagatt tttcgcagac
      241 tcactgaggg aaaagtcgtt gtcatggggc gaaagacctt tgagtctatc ggcaagcctc
      301 taccgaaccg tcacacattg gtaatctcac gccaagctaa ctaccgcgcc actggctgcg
      361 tagttgtttc aacgctgtcg cacgctatcg ctttggcatc cgaactcggc aatgaactct
      421 acgtcgcggg cggagctgag atatacactc tggcactacc tcacgcccac ggcgtgtttc
      481 tatctgaggt acatcaaacc ttcgagggtg acgccttctt cccaatgctc aacgaaacag
      541 aattcgagct tgtctcaacc gaaaccattc aagctgtaat tccgtacacc cactccgttt
      601 atgcgcgtcg aaacggctaa ccattccgtc aacgggacgc caa
//
LOCUS       DAZXUL010000455          427 bp    DNA     linear   BCT 28-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 16-00PHL-SF13
            SAMN39618784-rid23472993.guided.438, whole genome shotgun sequence.
ACCESSION   DAZXUL010000455 DAZXUL010000000
VERSION     DAZXUL010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618784
            Sequence Read Archive: SRR27734387
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394699.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394699.1
            Genome Coverage       :: 49x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:43:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,248
            CDSs (with protein)               :: 4,248
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 492
            CDSs (without protein)            :: 492
            Pseudo Genes (ambiguous residues) :: 0 of 492
            Pseudo Genes (frameshifted)       :: 224 of 492
            Pseudo Genes (incomplete)         :: 176 of 492
            Pseudo Genes (internal stop)      :: 164 of 492
            Pseudo Genes (multiple problems)  :: 67 of 492
            Pseudo Genes (short protein)      :: 1 of 492
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618784-rid23472993.guided.438"
                     /isolate="16-00PHL-SF13"
                     /isolation_source="Isolate - Feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            69..416
                     /locus_tag="ACH3L1_004833"
     CDS             69..416
                     /locus_tag="ACH3L1_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFT4079186.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
BASE COUNT           85 a           98 c          108 g          136 t
ORIGIN      
        1 caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga
       61 gatatatcat gaaaggctgg ctttttcttg ttatcgcaat agttggcgaa gtaatcgcaa
      121 catccgcatt aaaatctagc gagggcttta ctaagcttgc cccttccgcc gttgtcataa
      181 tcggttatgg catcgcattt tattttcttt ctctggttct gaaatccatc cctgtcggtg
      241 ttgcttatgc agtctggtcg ggactcggcg tcgtcataat tacagccatt gcctggttgc
      301 ttcatgggca aaagcttgat gcgtggggct ttgtaggtat ggggctcata attgctgcct
      361 ttttgctcgc ccgatcccca tcgtggaagt cgctgcggag gccgacgcca tggtgacggt
      421 gttcggc
//
